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From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sat, 1 Jan 2005 08:29:37 -0600
Subject: [Microscopy] Administrivia: December Archives now on-line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Happy New Year Colleagues!

The Archives for all of 2004 are now on-line at:

http://www.microscopy.com/MicroscopyListserver


Cheers
Nestor
Your Friendly Neighborhood SysOp



From MicroscopyL-request-at-ns.microscopy.com Sun Jan 2 08:48:43 2005



From: innap-at-savion.cc.huji.ac.il (by way of MicroscopyListserver)
Date: Sun, 2 Jan 2005 08:54:26 -0600
Subject: [Microscopy] viaWWW: imaging of microemulsions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (innap-at-savion.huji.ac.il) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Sunday, January 2, 2005 at 06:56:05
---------------------------------------------------------------------------

Email: innap-at-savion.huji.ac.il
Name: Inna Popov

Organization: The Unit for Nanoscopic Characterization, The Hebrew University of Jerusalem

Title-Subject: [Microscopy] [Filtered] MListserver: Need help in imaging of microemulsions

Question: Dear Listers,
I need your help in imaging microemulsions of types oil-in-water/water-in-oil with oil drops of 10 to 50 nm size.
Do you have any experience in SEM/ESEM/CryoSEM imaging of such objects?

Thank you in advance,
Inna

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sun Jan 2 13:07:07 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 03 Jan 2005 08:12:28 +1300
Subject: [Microscopy] 840 CL alignment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year

Many thanks to those who replied to my earlier posting regarding CL alignment on a
JEOL 840, but please note that it's the CL alignment specifically (the bit you have to do
after tearing down the column) that I'm having problems with, not the objective aperture
or stigmator adjustment.

I'm still seeking understanding of that part (ie the CL alignment), if anyone has that one
sorted.

thanks and all the best for 2005

cheers

rtch



--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 3 08:19:47 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 3 Jan 2005 08:24:40 -0600
Subject: [Microscopy] viaWWW: imaging of microemulsions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Inna,

If you have access to an SEM with a liquid nitrogen-cooled cryostage and
a way of plunge freezing samples and transferring them frozen into the
specimen chamber, this would be a good way to do it. I have imaged
foamy liquids with great success like this, and the process was very
simple.

Briefly, the liquid to be imaged was shaken violently to form the
bubbles, then a drop of the foam was put on the specimen holder and
plunged into liquid nitrogen. I was using an EMITech cryopreparation
unit, so I took the sample out of the LN2 bath under vacuum and
transferred it to the fracture and coating stage of the unit. Using a
built-in pick, I fractured off a piece of the frozen foam, then coated
the sample with gold before transferring it under vacuum to the SEM
stage. Micrographs were taken with no problems.

This was actually one of the easiest cryo specimens I ever worked with,
and I expect yours would work much the same way. My only concern might
be the size of the droplets you want to image, since cryoSEM, in my
experience, doesn't have the resolution of "normal" SEM work. If you
have any questions, please let me know.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
} From: by way of MicroscopyListserver [mailto:innap-at-savion.cc.huji.ac.il]

Sent: Sunday, January 02, 2005 8:54 AM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (innap-at-savion.huji.ac.il) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Sunday,
January 2, 2005 at 06:56:05
------------------------------------------------------------------------
---

Email: innap-at-savion.huji.ac.il
Name: Inna Popov

Organization: The Unit for Nanoscopic Characterization, The Hebrew
University of Jerusalem

Title-Subject: [Microscopy] [Filtered] MListserver: Need help in imaging
of microemulsions

Question: Dear Listers,
I need your help in imaging microemulsions of types
oil-in-water/water-in-oil with oil drops of 10 to 50 nm size.
Do you have any experience in SEM/ESEM/CryoSEM imaging of such objects?

Thank you in advance,
Inna

------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Mon Jan 3 15:36:23 2005



From: Alberto Diaspro :      diaspro-at-fisica.unige.it
Date: Mon, 3 Jan 2005 22:40:04 +0100
Subject: [Microscopy] Fwd: Content Alert: Microscopy Research and Technique 65, 4-5

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Inizio del messaggio inoltrato:

} Da: WileyInterScienceAlerts-at-wiley.com
} Data: 03 gennaio 2005 21:25:06 CET
} A: (alert recipient)
} Oggetto: Content Alert: Microscopy Research and Technique 65, 4-5
}
} Microscopy Research and Technique
} Volume 65, Issue 4-5, 2004.
}
} Online ISSN: 1097-0029
} Print ISSN: 1059-910X
} (Special Issue: A Nanoworld Under the Microscope - From Cell
} Trafficking to Molecular Machines. Issue Edited by Alberto Diaspro.)
}
}
} Copyright © 2004 Wiley-Liss, Inc., A Wiley Company
}
} -----------------------------------------------------------------------
} ----
}
} Pages: 167-168
} Introduction: A nanoworld under the microscope - From cell trafficking
} to molecular machines
} Alberto Diaspro
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861800/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20137
}
} Pages: 169-179
} Ensemble and single particle photophysical properties (two-photon
} excitation, anisotropy, FRET, lifetime, spectral conversion) of
} commercial quantum dots in solution and in live cells
} H.E. Grecco, K.A. Lidke, R. Heintzmann, D.S. Lidke, C. Spagnuolo, O.E.
} Martinez, E.A. Jares-Erijman, T.M. Jovin
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861786/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20129
}
} Pages: 180-185
} Super-resolution bright-field optical microscopy based on nanometer
} topographic contrast
} Shu-Wei Huang, Hong-Yao Mong, Chau-Hwang Lee
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861787/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20091
}
} Pages: 186-193
} Single molecule spectroscopic characterization of GFP-mut2 mutant for
} two-photon microscopy applications
} Fabio Cannone, Michele Caccia, Sara Bologna, Alberto Diaspro, Giuseppe
} Chirico
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861788/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20125
}
} Pages: 194-204
} Exploring molecular motors and switches at the single-molecule level
} M. Capitanio, F. Vanzi, C. Broggio, R. Cicchi, D. Normanno, G. Romano,
} L. Sacconi, F.S. Pavone
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861789/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20126
}
} Pages: 205-217
} Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes
} Maria Luisa Barcellona, Seth Gammon, Theodore Hazlett, Michelle A.
} Digman, Enrico Gratton
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861801/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20121
}
} Pages: 218-225
} Diffusion of microspheres in sealed and open microarrays
} B. Rieger, H.R.C. Dietrich, L.R. Van Den Doel, L.J. Van Vliet
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861802/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20128
}
} Pages: 226-234
} Chemical and thermal denaturation of crystalline bacterial S-layer
} proteins: An atomic force microscopy study
} José L. Toca-Herrera, Susana Moreno-Flores, Jacqueline Friedmann,
} Dietmar Pum, Uwe B. Sleytr
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861803/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20127
}
} Pages: 235-245
} Single molecule studies of RNA secondary structure: AFM of TYMV viral
} RNA
} Andrea Giro, Anna Bergia, Giampaolo Zuccheri, Hugo H.J. Bink, Cornelis
} W.A. Pleij, Bruno Samorì
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861804/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20123
}
} Pages: 246-251
} Monitoring of glass derivatization with pulsed force mode atomic force
} microscopy
} Andreas Ebner, Ferry Kienberger, Cordula M. Stroh, Hermann J. Gruber,
} Peter Hinterdorfer
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861805/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20124
}
} Pages: 252-262
} Microscopy of biological sample through advanced diffractive optics
} from visible to x-ray wavelength regime
} Enzo Di Fabrizio, Dan Cojoc, Valentina Emiliani, Stefano Cabrini,
} Maite Coppey-Moisan, Enrico Ferrari, Valeria Garbin, Matteo Altissimo
} http://www3.interscience.wiley.com/cgi-bin/abstract/109861806/ABSTRACT
}
} Published Online: 3 Jan 2005
} DOI: 10.1002/jemt.20122
}
}
}
} -----------------------------------------------------------------------
} ----
} Copyright (c) 1999-2004 by John Wiley & Sons, Inc. All rights reserved.
}
}
}
}
------------------------------------------------------------------------
---------------------------
Alberto Diaspro, Department of Physics, University of Genoa
Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309
URL: http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html
------------------------------------------------------------------------
--------------------------




From MicroscopyL-request-at-ns.microscopy.com Mon Jan 3 18:40:56 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Tue, 04 Jan 2005 18:45:34 -0600
Subject: [Microscopy] Re: RE: viaWWW: imaging of microemulsions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Inna

The new CytoViva system which debuted last month at Cell Bio, would also give you an interesting approach, with much less sample prep.

The system provides both contrast and high resolution. It retrofits easily to existing light microscopes (research stands) and I've seen it work quite well with colloids on the order of 50nm. The neat thing for emulsions is that you can observe them in real time.

If you are interested, take a look at their website : www.CytoViva.com. Although the applications shown there are biological, I've worked with emulsions before and the imaging principles are very similar. I'd also recommend your contacting Dr. Tom Hasling at Aetos (the manufacturer) and ask him to run a sample for you (+1 334-749-0134). If you can't send him something directly, perhaps you can suggest an emulsion system which is similar to yours.

Hope this is helpful.

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.

Caveat: MME was part of the initial launch team for this product.



At 08:24 AM 1/3/2005, Tindall, Randy D. wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Mon Jan 3 18:42:58 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Tue, 04 Jan 2005 18:47:38 -0600
Subject: [Microscopy] Re: RE: viaWWW: imaging of microemulsions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Inna

The new CytoViva system which debuted last month at Cell Bio, would also give you an interesting approach, with much less sample prep.

The system provides both contrast and high resolution. It retrofits easily to existing light microscopes (research stands) and I've seen it work quite well with colloids on the order of 50nm. The neat thing for emulsions is that you can observe them in real time.

If you are interested, take a look at their website : www.CytoViva.com. Although the applications shown there are biological, I've worked with emulsions before and the imaging principles are very similar. I'd also recommend your contacting Dr. Tom Hasling at Aetos (the manufacturer) and ask him to run a sample for you (+1 334-749-0134). If you can't send him something directly, perhaps you can suggest an emulsion system which is similar to yours.

Hope this is helpful.

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.

Caveat: MME was part of the initial launch team for this product.



At 08:24 AM 1/3/2005, Tindall, Randy D. wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Mon Jan 3 22:38:47 2005



From: George Theodossiou :      George.Theodossiou-at-amcor.com.au
Date: Tue, 4 Jan 2005 15:44:40 +1100
Subject: [Microscopy] Etch Solution for Ni

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

Happy New Year...Hope you are all well and still on holidays instead of
being back at work. I wish I was.

Now to my question....This is probably a silly question but its better to
ask. Can anyone recommend an etch solution to reveal the grain structure of
electro plated Ni. I know of Kalling's No. 2 and Acetic Glyceregia for Ni
based alloys. I expect them to work on Ni but just wanted to be sure and
find out if anyone can recommend at better solution.

Regards
George

George Theodossiou
Physicist / Microscopist
Microscopy and Microanalysis Laboratory

AMCOR Research and Technology
Ph: +61 3 9490 6135
Fax: +61 3 9499 4295
Mobile: 0409 568 840
email: George.Theodossiou-at-amcor.com.au


************************************************************************
CAUTION - This message may contain privileged and confidential
information intended only for the use of the addressee named above.
If you are not the intended recipient of this message you are hereby
notified that any use, dissemination, distribution or reproduction of
this message is prohibited. If you have received this message in error
please notify AMCOR immediately.
Any views expressed in this message are those of the individual sender
and may not necessarily reflect the views of AMCOR.
************************************************************************



From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 00:47:11 2005



From: hanke :      hanke-at-mee-inc.com
Date: Tue, 04 Jan 2005 02:08:36 -0500
Subject: [Microscopy] Re: Etch Solution for Ni

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The etchant that I prefer for electoplated nickel is a 1:1 mixture of acetic
and nitric acids.

George Theodossiou writes:

} Can anyone recommend an etch solution to reveal the grain structure of
} electro plated Ni. I know of Kalling's No. 2 and Acetic Glyceregia for Ni
} based alloys. I expect them to work on Ni but just wanted to be sure and
} find out if anyone can recommend at better solution.
}
} Regards
} George
}
} George Theodossiou
} Physicist / Microscopist
} Microscopy and Microanalysis Laboratory
}



--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 02:33:17 2005



From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Tue, 04 Jan 2005 09:38:29 +0100
Subject: [Microscopy] Re: Etch Solution for Ni

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} From Etchants Database, http://www.kaker.com/Etch/Etch.html :

Material: Nickel and Nickel Alloys
Type: Polishing
Method: Electropolishing
Etchant (Electrolyte): 37 ml H3PO4 (conc.), 56 ml glycerol, 7 ml water.
Procedure: 9-10 A/in2, Pt cathode, water cooled.
Remarks: Nickel 200.
Reference: Metallography, Structures and Phase Diagrams, Metals Handbook, 8th
Edition, Vol. 8, ASM (American Society for Metals), Metals Park, Ohio 44079, USA,
1973, p. 137.

Material: Nickel and Nickel Alloys
Type: Macro
Method: Chemical etching
Etchant (Electrolyte): 25 ml nitric acid (conc.), 75 ml hydrofluoric acid (conc.).
Procedure: Etching time is 3-10 min. Use fume cupboard.
Remarks: Ni-Cr and Ni-Cr-Fe alloys.
Reference: H. Modin and S. Modin, Metallurgical Microscopy, Butterworths, London,
1973., p. 390.

Material: Nickel and Nickel Alloys
Type: Polishing
Method: Electropolishing
Etchant (Electrolyte): 37 ml H3PO4 (conc.), 56 ml glycerol, 7 ml water.
Procedure: 8-10 A/in2, Pt cathode, water cooled.
Remarks: Duranickel 301.
Reference: Metallography, Structures and Phase Diagrams, Metals Handbook, 8th
Edition, Vol. 8, ASM (American Society for Metals), Metals Park, Ohio 44079, USA,
1973, p. 137.

Material: Nickel Specimens and Nickel Alloys
Type: Micro
Method: Electro polihsing
Etchant (electrolyte): A.) For Ni: 50 % hydrochloric aicd, 25 % water. B.) For Ni
alloys: 75 % hydrochloric acid, 25 % water.
Procedure: No data.
Remarks: Jet electro polishing for discs.
Reference: S.Mader, et.al., J.App.Phys., Vol.34, 1963, p.3376.

Henrik Kaker


George Theodossiou wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi All,
}
} Happy New Year...Hope you are all well and still on holidays instead of
} being back at work. I wish I was.
}
} Now to my question....This is probably a silly question but its better to
} ask. Can anyone recommend an etch solution to reveal the grain structure of
} electro plated Ni. I know of Kalling's No. 2 and Acetic Glyceregia for Ni
} based alloys. I expect them to work on Ni but just wanted to be sure and
} find out if anyone can recommend at better solution.
}
} Regards
} George
}
} George Theodossiou
} Physicist / Microscopist
} Microscopy and Microanalysis Laboratory
}
} AMCOR Research and Technology
} Ph: +61 3 9490 6135
} Fax: +61 3 9499 4295
} Mobile: 0409 568 840
} email: George.Theodossiou-at-amcor.com.au
}
} ************************************************************************
} CAUTION - This message may contain privileged and confidential
} information intended only for the use of the addressee named above.
} If you are not the intended recipient of this message you are hereby
} notified that any use, dissemination, distribution or reproduction of
} this message is prohibited. If you have received this message in error
} please notify AMCOR immediately.
} Any views expressed in this message are those of the individual sender
} and may not necessarily reflect the views of AMCOR.
} ************************************************************************

--
Dr. Henrik Kaker
Metal Ravne
SEM-EDS Laboratory
Koroska cesta 14
SI-2390 Ravne
Slovenia
Phone: +386 2 870 7076
Fax: +386 2 870 7022
GSM: +386 31 380 875




From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 05:20:02 2005



From: Gerd Leitinger :      gerd.leitinger-at-meduni-graz.at
Date: Tue, 04 Jan 2005 12:23:00 +0100
Subject: [Microscopy] LM prepared slides of animal nervous system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

I am wondering whether anybody can help me obtaining microscopical
slides of the nervous system of classical experimental animals.

I will be teaching students in a course on histology of the nervous
system and am supposed to compare specimens of the human
nervous system with the nervous system of classical experimental
animals. We have many slides of the human nervous system here at
this department, but are lacking slides of other animals.
I myself work on insects, so it will be easy for me to prepare slides with
ganglia of insects, but for comparison, I shall need slides of other
invertebrates (squid, Aplysia, Lymnaea... ?).
Does anybody know a source of spare, prepared slides of parts of the
nervous system of such invertebrates?
Or, does anybody know a source of Aplysia or Lymnaea and have
dissection istructions, so that I can attempt to dissect them myself?

thank you

Gerd Leitinger

Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at




From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 06:40:49 2005



From: neil-at-young8696.freeserve.co.uk (by way of MicroscopyListserver)
Date: Tue, 4 Jan 2005 06:46:16 -0600
Subject: [Microscopy] viaWWW: TEM School, Antwerp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (neil-at-young8696.freeserve.co.uk) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, January 4, 2005 at 05:55:02
---------------------------------------------------------------------------

Email: neil-at-young8696.freeserve.co.uk
Name: Neil

Organization: University of Birmingham

Title-Subject: [Microscopy] [Filtered] TEM School, Antwerp

Question: Hi, just thought I'd see if anyone is attending the winter school at Antwerp this month?

Neil Young
Nanophysics Research Laboratory
University of Birmingham

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 16:03:59 2005



From: jfrancis-at-powell.k12.ky.us (by way of Ask-A-Microscopist)
Date: Tue, 4 Jan 2005 16:09:37 -0600
Subject: [Microscopy] AskAMicroscopist: microscope that we can view on a tv or

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jfrancis-at-powell.k12.ky.us) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 4, 2005 at 11:37:24
---------------------------------------------------------------------------

Email: jfrancis-at-powell.k12.ky.us
Name: Jennifer Francis

Organization: Powell County Schools

Education: 6-8th Grade Middle School

Location: Stanton,KY, USA

Question: I am formerly an elementary school teacher, now working with middle school students. I would like to invest in a microscope that we can view on a tv or computer monitor. Can you tell me what equipment I might need to do that? I am not sure what the differences are between a digital microscope, a flex cam, etc. The digital microscopes seem to be less expensive, and I wondering if I connect that to a computer/laptop if I could use a digital projector (already own) to project from there and if that is better than something like the flex cam? Would you have any ideas? Thanks!

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 16:08:17 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Tue, 4 Jan 2005 16:14:19 -0600
Subject: [Microscopy] Administrivia: Final Notice to All Subscribers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


==============================================
Thrid and Final Notice of Microscopy Listserver DataBase Update:
==============================================
Jan 4, 2005

Colleagues, if you have subscribed/resubscribed since Dec. 21st
You may ignore the remainder of this message you will already
be in the new database. The Listserver will begin using the new database
records next week.

=======================================
Second Notice of Microscopy Listserver DataBase Update:
=======================================
Dec. 28, 2004
=======================================
First Notice of Microscopy Listserver DataBase Update:
=======================================
Dec. 21, 2004

Colleagues....

After nearly a dozen years of operation, the master database of the
Microscopy Listserver needs a serious reworking and purging. I have done
the hard work of restructuring the software model but now the
database needs to be "repopulated" with updated/new user information.

The easiest way for me to accomplish this is to simply purge the entire database,
and have each of you resubscribe as if you are a new user.

As a result, I'm asking EACH and EVERY ONE of you to visit the following WWW site
and fill out the new subscription form. Here is the URL:

http://www.microscopy.com/MicroscopyListserver/SubscribeMicroscopy.html

You can do this beginning immediately, the changes will be stored and
I will implement the new system on or about the second week of Jan. 2005.
If all goes well, resubscribing should only take a few minutes of your time,
so please do it as soon as possible.

For the balance of 2004 the old database will remain in use, but
if you have not resubscribed by ~ the first week of Jan 2005,
you will no longer receive Listserver Email as I will cease using the
old database and only forward Listserver Email to those individuals who have
subscribed and are recorded in the new system.

I'll post this message every Monday through Jan 3, as a reminder and shortly thereafter will
transition away from the old database to the new one.

Thanks in advance for your patience and cooperation.

Nestor
Your Friendly Neighborhood SysOp.


From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 16:57:04 2005



From: Stacey.Andringa-at-uc.edu (by way of MicroscopyListserver)
Date: Tue, 4 Jan 2005 17:02:44 -0600
Subject: [Microscopy] viaWWW: database of TEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, January 4, 2005 at 08:24:11
---------------------------------------------------------------------------

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Microscopy] [Filtered] image database

Question: My co-worker has a proposal for a massive database of
TEM images. Here it is.

I am interested in finding (or creating) an easily accesible
transmission electron microscopic image database which will
have thousands of images available to preview....without
comments or arrows or descriptions on the actual images....
but with the option to retrieve the article or a description
of the treatment/species/etc on a separate link. There
could be an image preview mode, and a high resolution link
and from there a link to the specifics of species and
treatment etc and/or an article on PubMed or Medline. The
database should be open to the public, and have a mechanism
for adding images to the database (in a particular format)
through fields just like filling out a form. The sorting of
images could be by mesh headings. The databases which I
have been able to access do not appear in this format, and
there is too much in the way of "other links and
descriptors" to allow for a quick visual scan of the images.
Does anyone out there have an interest in such a database...
and would anyone out there be willing to be co-investigator
on a grant to prepare and maintain such a database.
Thanks for your responses.

marian.miller-at-uc.edu




---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 18:04:57 2005



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Tue, 4 Jan 2005 18:10:07 -0600
Subject: [Microscopy] Database for TEM Images etc....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stacey

There are alot of possible image databases out there both commerical and public-domain.
None that I know of do exactly what you ask, but then that is why you asked for input isn't it?

I'll point you to the public-domain TPM Electronic Notebook at :

http://tpm.amc.anl.gov

As an electronic notebook, it does alot of what you asked for and technically it is simply
a database with a WWW based GUI front end. As a Notebook it handles not only text but
also images. It is searchable and can be customized with a modicum of effort to incorporate nearly
everything you asked about.

If your interested in fiddling, go to the WWW site, then select ENotebook Button, then choose the
PUBLIC Notebook and "Enter the ENotebook. Both public and private ENotebooks
are available, the difference being simply login requirements.

Each "record" in the Notebook is a seperate page, but the pages can be of unlimited length and data can
be added to any individual pages at any time (dated and time stamped of course).

A customizable thumbnail page can be made to show summary images (Table of Contents). Which will give
you a preview of multiple pages.

There are some example images in the public notebook. Comment fields, form based input,
various file formats supported (JPG/TIF/GIF/BMP/PNG/QT/MOV/MPG/AVI/PDF/DOC/XLS/PPT/ASCII/Binary )
thumbnails and full view images. Links and articles describing the images are appendable to the
page, or can be embedded on the page itself.

The ENotebook is (obviously) WWW based and currently runs on a Linux Server. This is part of the
TelePresence Microscopy Collaboratory Project at Argonne National Lab. A single server
can support multiple private and/or public ENotebooks. In principle the only limitation
to the number of images is the size of your Disk Drive.

Rumor has it I know the developer, so get back to me if you have any questions.

Nestor
Your Friendly Neighborhood SysOp


}
} Email: Stacey.Andringa-at-uc.edu
} Name: Stacey Andringa
}
} Organization: University of Cincinnati
}
} Title-Subject: [Microscopy] [Filtered] image database
}
} Question: My co-worker has a proposal for a massive database of
} TEM images. Here it is.
}
} I am interested in finding (or creating) an easily accesible
} transmission electron microscopic image database which will
} have thousands of images available to preview....without
} comments or arrows or descriptions on the actual images....
} but with the option to retrieve the article or a description
} of the treatment/species/etc on a separate link. There
} could be an image preview mode, and a high resolution link
} and from there a link to the specifics of species and
} treatment etc and/or an article on PubMed or Medline. The
} database should be open to the public, and have a mechanism
} for adding images to the database (in a particular format)
} through fields just like filling out a form. The sorting of
} images could be by mesh headings. The databases which I
} have been able to access do not appear in this format, and
} there is too much in the way of "other links and
} descriptors" to allow for a quick visual scan of the images.
} Does anyone out there have an interest in such a database...
} and would anyone out there be willing to be co-investigator
} on a grant to prepare and maintain such a database.
} Thanks for your responses.
}
} marian.miller-at-uc.edu
}
}
}
}
} ---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 18:17:31 2005



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 04 Jan 2005 19:22:42 -0500
Subject: [Microscopy] Olympus BX61 computer control question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have an Olympus BX61 that we are using alternating control of from one
software package to another. One of the software packages is proprietary
and must have the dip switches on the microscope communications hardware
set a certain way.

Does anybody know what these switch setting mean so that we can attempt to
connect with the other software which may be flexible?

I posted images of the problem at
http://www.aecom.yu.edu/aif/temp/sky/index2.htm

Any help would be greatly appreciated.

Thank you.

-Michael
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**



From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 18:53:24 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 4 Jan 2005 17:11:06 -0800
Subject: [Microscopy] Re: AskAMicroscopist: microscope that we can view on a tv or computer monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 4, 2005, at 2:09 PM, by way of Ask-A-Microscopist wrote:

} Question: I am formerly an elementary school teacher, now working with
} middle school students. I would like to invest in a microscope that
} we can view on a tv or computer monitor. Can you tell me what
} equipment I might need to do that? I am not sure what the differences
} are between a digital microscope, a flex cam, etc. The digital
} microscopes seem to be less expensive, and I wondering if I connect
} that to a computer/laptop if I could use a digital projector (already
} own) to project from there and if that is better than something like
} the flex cam? Would you have any ideas? Thanks!
}
Dear Jennifer,
Caroline Schooley is an expert on this; her contact info and the web
site for Project MICRO are:

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates:
http://www.fortbragg.k12.ca.us/AG/marinelab.html

Project MICRO's purpose is to enhance pre-college education, as stated
on the web page.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Jan 4 20:22:03 2005



From: Elaine Humphrey :      ech-at-interchange.ubc.ca
Date: Tue, 04 Jan 2005 18:27:15 -0800
Subject: [Microscopy] Re: viaWWW: database of TEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stacey
We have such a data base at UBC. Dr. Lacey Samuels instigated it as a
resource for a Bio200 cell biology course and Joseph Dietz designed
it. It just grew from there. Anyone can add images to it, and we
encourage users of this facility to "add a useful image." You can
access it from my website http://www.emlab.ubc.ca On the left hand
side is the link to the BioMedia Database. There is a very easy
search engine.

We have the policy that if the images are to be used for educational
purposes and non-profit then you can download them for free. If they
are to be used for profit, then the copyright stays with the author.

We are at present setting up the link to the protocols pages on the
emlab website for the images in the database.

Let me know if you would like to add images to it and need more information.
Elaine



} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca


From MicroscopyL-request-at-ns.microscopy.com Wed Jan 5 07:44:54 2005



From: Richard Edelmann :      edelmare-at-MUOhio.edu
Date: Wed, 5 Jan 2005 08:49:08 -0500
Subject: [Microscopy] Re: Olympus BX61 computer control question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Michael:

Seeing that you are in the Bronx, why don't you just call out to Melville and
ask them? They are very freindly and should be able to fax you off the dip
switch info.

Corporate Headquarters:
Olympus America Inc.
2 Corporate Center Drive P.O. Box 9058
Melville, NY 11747-9058
1-800-645-8160


}
} We have an Olympus BX61 that we are using alternating control of from one
} software package to another. One of the software packages is proprietary
} and must have the dip switches on the microscope communications hardware
} set a certain way.
}
} Does anybody know what these switch setting mean so that we can attempt to
} connect with the other software which may be flexible?
}
} I posted images of the problem at
} http://www.aecom.yu.edu/aif/temp/sky/index2.htm
}
} Any help would be greatly appreciated.
}
} Thank you.
}
} -Michael
} ____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
} (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
} **This electronic transmission contains information that is privileged.**
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From MicroscopyL-request-at-ns.microscopy.com Wed Jan 5 11:29:49 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 05 Jan 2005 11:34:09 -0600
Subject: [Microscopy] Re: AskAMicroscopist: microscope that we can

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Jennifer,

You can actually turn nearly any microscope into a digital microscope by attaching a camera to it. Several companies which come to mind which have cameras that can slip right into the place where a normal eyepiece goes are VideoLab and Ken-a-Vision. I'd also suggest seeing if Swift Instruments has anything.

Several years ago, we taught a week-long course for jr. high school and high school teachers (ironically, chemistry and physics) at Miami University (Oxford, OH). Early in the program, we put these small, inexpensive cameras (I think, at that time, that the VideoLab camera only cost about $258 for a direct feed into a video monitor... maybe another $125 for a card to go into a computer, if you wanted to capture images) on the normal, high-school level microscopes and ran experiments on polarized light, diatoms, and contrast techniques (ex: salt crystals under Darkfield and a technique popular in the 1860's called Rheinberg), as well as calibration experiments and measurements. The teachers were incredibly impressed with the ability to transfer the image from the microscope to the video monitor. (Actually, they all instantly reverted from being mature, in-control adults to enthusiastic 7 year-olds! ... and I mean that in the most complimentary sense).

As for equipment:
For the simplest connection, just the camera and a video monitor. the cameras typically come with some sort of clamping device. Just remove the eyepiece and replace with the camera and adapter.

For the next level up, you can get an analog-to-digital (ADC) video capture card that goes into the computer. The camera then hooks into the back of the card and the card, in conjunction with some special software, captures the images and makes them available for storage and manipulation in the computer.

Although I've not researched the market lately, I think that all of the inexpensive cameras (like the Flexcam), are analog. They require both the electronic interface and software to communicate with a computer and produce digital images.

Once you have the images in digital format, you can use your computer and digital projector to project the still images in the classroom. Alternatively, using the simple set up described above, you can project live moving objects, like pond critters and crystal growth.

I think you will find a web search and the camera manufacturers a wealth of info, but contact me off-line if you have further questions.

Good hunting! .... and have fun!!!

Best regards,
Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
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Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.



At 04:09 PM 1/4/2005, jfrancis-at-powell.k12.ky.us wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Wed Jan 5 12:02:31 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 5 Jan 2005 10:06:52 -0800
Subject: [Microscopy] AskAMicroscopist: microscope that we can view on a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jennifer;
A number of years ago, Intel and Mattel teamed up to produce a
very inexpensive microscope that interfaced to a computer.
Unfortunately, they ceased production, although they can be found on
Ebay. (I just checked-there are 16 on Ebay at this moment, with the
highest price being $40) I regret that I never bought one. Everyone I
know who bought one for their kids was delighted. BTW, I do work for
Intel, but have I no connection at all to this microscope, and as I
said, it is out of production.

John Mardinly

-----Original Message-----
} From: by way of Ask-A-Microscopist [mailto:jfrancis-at-powell.k12.ky.us]
Sent: Tuesday, January 04, 2005 2:10 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jfrancis-at-powell.k12.ky.us) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, January 4, 2005 at 11:37:24
------------------------------------------------------------------------
---

Email: jfrancis-at-powell.k12.ky.us
Name: Jennifer Francis

Organization: Powell County Schools

Education: 6-8th Grade Middle School

Location: Stanton,KY, USA

Question: I am formerly an elementary school teacher, now working with
middle school students. I would like to invest in a microscope that we
can view on a tv or computer monitor. Can you tell me what equipment I
might need to do that? I am not sure what the differences are between a
digital microscope, a flex cam, etc. The digital microscopes seem to be
less expensive, and I wondering if I connect that to a computer/laptop
if I could use a digital projector (already own) to project from there
and if that is better than something like the flex cam? Would you have
any ideas? Thanks!

------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Wed Jan 5 12:31:52 2005



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 5 Jan 2005 10:00:14 -0800
Subject: [Microscopy] Re: AskAMicroscopist: microscope that we can view

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Jennifer -

The answer to your question has to start with the politics of
education. Digital microscopes with a computer feed qualify for
"technology" funds, which makes them easier to purchase in many
school districts. Your classroom computer must be new enough to have
a USB port. Yes, the projector will work fine. Flex cams are
available with either analog (TV) or digital (PC) feed, and are a
good choice if you already have a microscope; they start at around
$300. An advantage of the flex cam approach is that it will work
with both compound and dissecting scopes, and many of the things that
middle school students want to observe (bugs, rocks, flowers, etc.)
are best observed with a dissecting scope. A basic monocular
dissecting scope costs $75.

That said, I urge you to think about what your students will see with
that digital projector; another image on a monitor, passively. From
the Project MICRO point of view, I'd like THEM to be the observers!
At the very least, buy a dozen or so "flashlight" style 30x
microscopes (~$10 each) as a supplement, so that each student can
learn to observe. You may find the advice on buying school
microscopes that is part of the MICRO web page helpful (URL below).
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/


From MicroscopyL-request-at-ns.microscopy.com Wed Jan 5 15:17:28 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Wed, 05 Jan 2005 15:22:31 -0600
Subject: [Microscopy] RE: AskAMicroscopist: microscope that we can view on a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

This microscope is still available, now from Digital Blue:
http://www.playdigitalblue.com/products/
There's the old QX3, and a new "improved" QX5. The quote marks are
because the QX5 no longer supports TWAIN, so it can't be run through,
say, Photoshop, like the QX3 can be -- the 5 only runs with the
proprietary software. Pity, that.

Phil

} Jennifer;
} A number of years ago, Intel and Mattel teamed up to produce a
} very inexpensive microscope that interfaced to a computer.
} Unfortunately, they ceased production, although they can be found on
} Ebay. (I just checked-there are 16 on Ebay at this moment, with the
} highest price being $40) I regret that I never bought one. Everyone I
} know who bought one for their kids was delighted. BTW, I do work for
} Intel, but have I no connection at all to this microscope, and as I
} said, it is out of production.
}
} John Mardinly
}
} -----Original Message-----
} } From: by way of Ask-A-Microscopist [mailto:jfrancis-at-powell.k12.ky.us]
} Sent: Tuesday, January 04, 2005 2:10 PM
} To: microscopy-at-microscopy.com
} Subject: [Microscopy] AskAMicroscopist: microscope that we can view on a
} tv or computer monitor
}
}
}
} ------------------------------------------------------------------------
} ------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From MicroscopyL-request-at-ns.microscopy.com Wed Jan 5 16:05:21 2005



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Wed, 05 Jan 2005 17:37:46 -0500
Subject: [Microscopy] Re: RE: AskAMicroscopist: microscope that we

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I second this recommendation for the Intel QX3, and if you are a mac
user there is a great freeware app called Mixscope that will capture
jpegs, tiffs, and timelapse quicktime movies from the QX3 on OS X.

http://homepage.mac.com/aireck/qx3/

best regards,
kevin

----- Original Message -----
} From: "Mardinly, John" {john.mardinly-at-intel.com}

I have one of these Intel 'scopes and am very unhappy with it. We never
use it. A cheap video camera and a monitor is so much better.

Here's a really simple and cheap approach (certainly easier than the BX61
we're trying to set up!):
http://www.aecom.yu.edu/aif/gallery/cheapscope/index.htm
which also shot the pictures, but with a 3 megapixel camera, at
http://www.flushart.com/gla/livinglake/index.htm

Regardless, based on my experience having done a project with two second
grade classes in September, the kids really need to look into the barrel of
the microscope themselves to get excited.

-Michael C.


} Jennifer;
} A number of years ago, Intel and Mattel teamed up to produce a
} very inexpensive microscope that interfaced to a computer.
} Unfortunately, they ceased production, although they can be found on
} Ebay. (I just checked-there are 16 on Ebay at this moment, with the
} highest price being $40) I regret that I never bought one. Everyone I
} know who bought one for their kids was delighted. BTW, I do work for
} Intel, but have I no connection at all to this microscope, and as I
} said, it is out of production.
}
} John Mardinly

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 5 16:44:01 2005



From: Cliff Glier :      cglier-at-opelco.com
Date: Wed, 5 Jan 2005 17:49:23 -0500
Subject: [Microscopy] Sales Positions Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

} Optical Elements Corporation (OPELCO) has current openings for a Light Microscope and a Confocal Microscope Sales Specialist in the Washington, DC region. If interested, please click on the following link or contact me directly.
} http://www.opelco.com/employmentcontact.htm
}
} Best Regards,
} Cliff Glier
} COO
} OPELCO
} 105 Executive Drive Suite 100
} Dulles, VA 20166
} 703.471.0080 x230
} 703.904.9432 (fax)
} cglier-at-opelco.com
} www.opelco.com




From MicroscopyL-request-at-ns.microscopy.com Wed Jan 5 16:44:22 2005



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 5 Jan 2005 14:47:17 -0800
Subject: [Microscopy] Looking for CdTe nannoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

I need some expert advice so I can help a user in my lab. He wants to see
some nannoparticles he has synthesized by the following procedure:

} The sample is CdTe, a highly fluorescent NP with a shell of either
} thioglycolic acid or 2-mercaptoethylamine. In theory they should be about
} 2-5nm in diameter, but they are synthesized in aqueous solution, and in
} order to properly seperate the particles I perform a ligand exchange and
} redissolve into organic solvents(ie toluene).

So, he shows up and I put his solution onto a carbon coated formvar grid. I
look around. I don't see much, some junk, but nothing like nannoparticles.
He is disappointed.

I am scratching my head. Is there something there and I can't see it? Would
I see it if it were there?

Maybe you have some ideas?

Would raw CdTe particles at 2 nm size have enough contrast to show up?

Could the solution be so concentrated that it looks like a solid field
rather than separate particles?

The solution he gave me didn't really dry on the grid like I thought it
would. How fast does toluene evaporate and could it mangle the formvar?

Any other helpful hints to get some results?

Thanks


Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Jan 5 21:26:44 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 5 Jan 2005 19:44:26 -0800
Subject: [Microscopy] Re: Looking for CdTe nannoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 5, 2005, at 2:47 PM, Jon Krupp wrote:

} I need some expert advice so I can help a user in my lab. He wants to
} see
} some nannoparticles he has synthesized by the following procedure:
}
} } The sample is CdTe, a highly fluorescent NP with a shell of either
} } thioglycolic acid or 2-mercaptoethylamine. In theory they should be
} } about
} } 2-5nm in diameter, but they are synthesized in aqueous solution, and
} } in
} } order to properly seperate the particles I perform a ligand exchange
} } and
} } redissolve into organic solvents(ie toluene).
}
} So, he shows up and I put his solution onto a carbon coated formvar
} grid. I
} look around. I don't see much, some junk, but nothing like
} nannoparticles.
} He is disappointed.
}
} I am scratching my head. Is there something there and I can't see it?
} Would
} I see it if it were there?
}
} Maybe you have some ideas?
}
} Would raw CdTe particles at 2 nm size have enough contrast to show up?
}
} Could the solution be so concentrated that it looks like a solid field
} rather than separate particles?
}
} The solution he gave me didn't really dry on the grid like I thought it
} would. How fast does toluene evaporate and could it mangle the formvar?
}
} Any other helpful hints to get some results?
}
Dear Jon,
I have had a few experiences looking at quantum dots and zeolite
precursors for some of the materials scientists and chemical engineers
here, and I may be able to answer some of your questions. First of
all, these objects are small and inherently hard to see, so it would be
easy to go to a relatively high mag, scan across a grid square or two
and not see much. I used cryofixation and looked at frozen-hydrated
material, which is generally lower contrast than particles on thin
carbon; however, the uniformity of the background could be better for
ice than for a carbon coat, and this would render the particles more
visible. CdTe certainly has more contrast than what I was looking at,
so that is not the problem. It is very unlikely that the material is
too concentrated to see. Especially since the evaporation of the
toluene was not as expected, the particles are much more likely to
aggregate than to form a uniform layer. If you have an oven or even a
warm room, you might try drying the toluene at a somewhat elevated
temperature. If the toluene mangled the formvar, I'd expect to see
prominent, irregular features (as happens when one gets poor formvar
removal using chloroform). If the particles as prepared are
well-dispersed in the aqueous solution, and if you have access to
cryopreparation equipment, you could try looking at plunge-frozen
specimens. Measuring the fluorescence of the solution should allow you
to calculate the number of particles per microliter, so you could
determine roughly how many particles you would expect to see in a field
at the magnification you use. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 06:49:39 2005



From: Thomas, Frank :      FThomas-at-nrcan.gc.ca
Date: Thu, 6 Jan 2005 08:55:03 -0400
Subject: [Microscopy] Sputter-coater recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers -

Does anyone have a recommendation for what make/model of sputter
coater to buy, if one was going to buy one? Currently our SEM stub coating
needs are met by an ancient Edwards evaporator (which is still working, by
the way). I know there's a number of other things one can do with an
evaporator that can't really be done with a sputter coater, but we don't do
those things - we really just need something to coat the occasional SEM
stub, in case the old Edwards unit finally buys the farm.
So if I was to have a small amount of capital dumped on me for such
a purchase, how much would I have to spend for a reasonably reliable little
sputter coater, and which one(s) should I consider?

Frank

F.C. Thomas
FThomas-at-NRCan.gc.ca, 902-426-4635, facsimile 902-426-6152
GSC Atlantic
Natural Resources Canada, Bedford Institute of Oceanography,
P.O. Box 1006, Dartmouth, Nova Scotia B2Y 4A2
Ressources naturelles Canada, l'Institut Oceanographique du Bedford, B.P.
1006, Dartmouth, (Nouvelle-Ecosse)
B2Y 4A2
Government of Canada/Gouvernement du Canada



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 08:29:24 2005



From: sbledsoe-at-iupui.edu (by way of MicroscopyListserver)
Date: Thu, 6 Jan 2005 08:34:37 -0600
Subject: [Microscopy] viaWWW: Image Database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sbledsoe-at-iupui.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, January 6, 2005 at 07:23:28
---------------------------------------------------------------------------

Email: sbledsoe-at-iupui.edu
Name: Sharon B Bledsoe

Organization: Indiana University, School of Medicine

Title-Subject: [Microscopy] [Filtered] MListserver: Image Database

Question: For a nice image database try Improvison's Volocity.

Volocity has a LE version that is free.

http://www.improvison.com

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 08:41:58 2005



From: Karen Bovard :      kbovard-at-creighton.edu
Date: Thu, 06 Jan 2005 08:44:43 -0600
Subject: [Microscopy] Image grabbing systems for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a JEOL 840A SEM and am interested in upgrading it to digital
photograpy capabilities.

I am aware of the Orion, SIS ADDA II, and the JEOL Orion systems.

Are there any different options (preferably cheaper) to consider?

Karen Bovard
EM Lab
Pathology
Creighton University
Omaha, NE



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 09:07:14 2005



From: Mary Ellen Pease :      MPEASE-at-jhmi.edu
Date: Thu, 06 Jan 2005 10:38:37 -0500
Subject: [Microscopy] tissue processing of cryosections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jonathon

I know that individual 5nm gold spheres start to become difficult to
see in a real sample on carbon/formvar grids. This is not so much size
as the density you will get against a relatively thick background
coating (} 50nm). Cd and Te are both lighter than Au and if the
particles are not too symmetrical then I would have thought that they
would be practically invisible.

It sounds like one of those classical e.m. problems where the
resolution of the microscope is not the issue but the resolution and
contrast of the sample may be. I suppose you could play around with the
voltage and aperture size to enhance contrast a bit, as well. Someone
has already mentioned shadowing but I wonder if a simple negative stain
might help - I really have no idea. But I'd be interested to hear how
you get on.

It would certainly be worth trying with the bare particles if you can.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk



----- Original Message -----
} From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)

Hi all,

I am looking for a protocol for epoxy processing of 8-10um previously
sectioned cryoembedded tissue. I last did this about 10 years ago and
can not locate my notes. I recall stepping the slide through the
processing solutions with shortened times in a covered coplin jar until
the final infiltration steps when the plastic was pipetted directly onto
the slide's surface over the section and placed under vacuum. I also
remember embedding by inverting the slide over an over filled beam
capsule, polymerizing, then separating the two by using LN2 to pop the
slide away from the plastic block.

If you have recommendations for the duration of the processing steps,
I'd appreciate hearing them.

Thank you,
Mary Ellen Pease

Mary Ellen Pease, M.S.
Laboratory Manager
Glaucoma Research Lab & Microscopy and Imaging Core Facility
Wilmer Eye Institute, Johns Hopkins Hospital
175 Woods Research
600 N. Wolfe Street
Baltimore, MD 21287
410-955-3337 (phone/voicemail)
443-287-2711 (fax)
mpease-at-jhmi.edu

WARNING: Email sent over the Internet is not secure. Information sent
by email may not remain confidential.
DISCLAIMER: This email is intended only for the individual to whom it
is addressed. It may be used only in accordance with applicable laws.
If you receive this email by mistake, notify the sender and destroy the
email.


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 09:54:29 2005



From: Gerroir, Paul :      paul.gerroir-at-xrcc.xeroxlabs.com
Date: Thu, 6 Jan 2005 10:57:27 -0500
Subject: [Microscopy] Sputter-coater recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank,
Although I may not be able to make an ultimate recommendation for
purchase I would suggest you avoid Cressington. We have their 208HR
model sputter coater with mtm20 thickness controller and have been
generally pleased with the operation of the unit. We use either Pt/Pd or
Cr targets to coat polymeric materials. What has been especially
unsatisfactory about the unit is its 'voracious appetite' for targets.
Typically our Pt/Pd targets (57mm dia. x 0.1mm thick) last 2 to 4 months
and our usage is not heavy. The unit's power is concentrated in a
central ring of about 20mm and when the targets fail a small
crescent-shaped hole remains where the Pt/Pd has been etched away. I
made some measurements of the actual consumption of Pt/Pd upon failure
and found it to be only 8 to 12%. When you are spending approx. $500 US
per target and you are left with about $450 US of waste Pt/Pd you can
see that it is a very inefficient use of your money. Apparently
Cressington have been developing a smaller magnetron head which would
accept a smaller diameter target and hence reduce the amount of waste
target material. One other dislike of the unit in its present
configuration is the inability to maintain vacuum in the chamber once
power is switched off. I am unaware if their newer models reflect
improvements in these two areas.

Good luck in your search.

Regards,
Paul


Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: 905-823-7091, ext.216
FAX: 905-822-7022
e-mail: paul.gerroir-at-xrcc.xeroxlabs.com

-----Original Message-----
} From: Thomas, Frank [mailto:FThomas-at-nrcan.gc.ca]
Sent: Thursday, January 06, 2005 7:55 AM
To: microscopy-at-microscopy.com

Listers -

Does anyone have a recommendation for what make/model of sputter
coater to buy, if one was going to buy one? Currently our SEM stub
coating needs are met by an ancient Edwards evaporator (which is still
working, by the way). I know there's a number of other things one can do
with an evaporator that can't really be done with a sputter coater, but
we don't do those things - we really just need something to coat the
occasional SEM stub, in case the old Edwards unit finally buys the farm.

So if I was to have a small amount of capital dumped on me for
such a purchase, how much would I have to spend for a reasonably
reliable little
sputter coater, and which one(s) should I consider?

Frank

F.C. Thomas
FThomas-at-NRCan.gc.ca, 902-426-4635, facsimile 902-426-6152 GSC Atlantic
Natural Resources Canada, Bedford Institute of Oceanography, P.O. Box
1006, Dartmouth, Nova Scotia B2Y 4A2 Ressources naturelles Canada,
l'Institut Oceanographique du Bedford, B.P.
1006, Dartmouth, (Nouvelle-Ecosse)
B2Y 4A2
Government of Canada/Gouvernement du Canada





From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 11:12:32 2005



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 06 Jan 2005 09:45:27 -0800
Subject: [Microscopy] new sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


----- Original Message -----
} From: "Mary Mager" {mager-at-interchange.ubc.ca}
To: "Karen Bovard" {kbovard-at-creighton.edu}
Sent: Thursday, January 06, 2005 9:15 AM

Dear Paul,
One solution to your problem is to contact Abe Dayani (tel. 702-368-0579) at
Refining Systems Inc. (http://www.refiningsystems.com/) for new sputtering
targets and a credit on the unused materials in your spent targets. He
prices his targets on the precious metal content and they are usually about
half the price of the targets from the manufacturer.
No financial interest, just a satisfied customer.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "Gerroir, Paul" {paul.gerroir-at-xrcc.xeroxlabs.com}
To: "Thomas, Frank" {FThomas-at-nrcan.gc.ca} ; {microscopy-at-microscopy.com}
Sent: Thursday, January 06, 2005 7:57 AM

Dear Frank,
I received a used Denton DeskII when I bought a used SEM and it has been a
good, solid performer. It is not fancy, but it is inexpensive, easy to
service and reliable.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 12:00:44 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Thu, 06 Jan 2005 12:05:59 -0600
Subject: [Microscopy] Re: Looking for CdTe nannoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

SEM or TEM? For either, we just deposit the particles on Formvar
coated grids, with or without C, directly from the water solution.
Just as long as their are no salts or the like to precipitate. This
works with colloidal particles down to 3 - 5 nm, and composed of Au,
Pt, Pd, Ag, Fe, and combinations and alloys of these.
The particles separate fine without doing anything else. This is also
true for particles conjugated to proteins like antibodies.
I did try to look at some home-made Cd/Se quantum dots that had been
synthesized in toluene (and a couple of other organic solvents, but I
forget which), and it was a no-go. The toluene attacked and either
dissolved or "wadded up" the formvar film. This may be what happened
to your samples.

Phil

} Hi:
}
} I need some expert advice so I can help a user in my lab. He wants to see
} some nannoparticles he has synthesized by the following procedure:
}
} } The sample is CdTe, a highly fluorescent NP with a shell of either
} } thioglycolic acid or 2-mercaptoethylamine. In theory they should be about
} } 2-5nm in diameter, but they are synthesized in aqueous solution, and in
} } order to properly seperate the particles I perform a ligand exchange and
} } redissolve into organic solvents(ie toluene).
}
} So, he shows up and I put his solution onto a carbon coated formvar grid. I
} look around. I don't see much, some junk, but nothing like nannoparticles.
} He is disappointed.
}
} I am scratching my head. Is there something there and I can't see it? Would
} I see it if it were there?
}
} Maybe you have some ideas?
}
} Would raw CdTe particles at 2 nm size have enough contrast to show up?
}
} Could the solution be so concentrated that it looks like a solid field
} rather than separate particles?
}
} The solution he gave me didn't really dry on the grid like I thought it
} would. How fast does toluene evaporate and could it mangle the formvar?
}
} Any other helpful hints to get some results?
}
} Thanks
}
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 14:04:49 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 06 Jan 2005 15:10:08 -0500
Subject: [Microscopy] JB-4 resin stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
I have a user who wants to stain lignin in JB-4 resin-embedded corn
stalks. He tried Phloroglucinol which works fine in paraffin embedded
samples since the paraffin is removed. However, this stain requires an HCl
treatment to reveal the desired color. The acid treatment interacts
adversely with the JB-4 resin.

The resin embedding gives much better resolution than the paraffin so it is
not an option to go back to the paraffin-embedded tissue.

Does anyone have an alternative stain that will work with this resin?

Thanks in advance,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy




From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 14:42:37 2005



From: Miller, F. Scott :      smiller-at-umr.edu
Date: Thu, 6 Jan 2005 14:46:54 -0600
Subject: [Microscopy] RE: Image grabbing systems for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Karen,

I am currently using the 4pi Revolution system to capture digital images on my JEOL 840 (and two other SEMs as well). You can find the information on the system at:

http://www.4pi.com/

I have been very pleased with the system for a number of years and also would point out that 4pi offers integrated EDS or WDS analysis with the imaging system.

Scott Miller
(No financial interest in 4pi, just a very satisfied customer)


F. Scott Miller, Ph.D.
Advanced Materials Characterization Lab
University of Missouri - Rolla
223 McNutt Hall
Rolla, MO 65409 USA
fax: 573 341 6934
voice: 573 341 4727


} ----------
} From: Karen Bovard
} Sent: Thursday, January 6, 2005 8:44 AM
} To: microscopy-at-msa.microscopy.com
} Subject: [Microscopy] Image grabbing systems for SEM
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} I have a JEOL 840A SEM and am interested in upgrading it to digital
} photograpy capabilities.
}
} I am aware of the Orion, SIS ADDA II, and the JEOL Orion systems.
}
} Are there any different options (preferably cheaper) to consider?
}
} Karen Bovard
} EM Lab
} Pathology
} Creighton University
} Omaha, NE
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 14:54:02 2005



From: Richard Harris :      rjharris-at-uwo.ca
Date: Thu, 6 Jan 2005 15:57:46 -0500
Subject: [Microscopy] Imapro QCRZi 35mm Film Recorder for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Imapro QCRZi 35mm Film Recorder. Comes with 35mm module & instruction
manuals. Utilizes GPIB interface. Has cables, software and instruction
manuals and PCI card from National Instruments. Running on Mac OS 9.2, the
RIP software is
Graphx RasterPlus includes all manuals. Includes Power Mac 8500 computer
and 19" Hitachi monitor.
In excellent working condition $500 USD or best offer.
Please contact Ian Craig directly at:
Ian Craig
Media Specialist
Faculty of Science
The University of Western Ontario
591-661-2111 ext.86778
icraig-at-uwo.ca


Richard Harris
Laboratory Supervisor
Department of Biology
University of Western Ontario
London Ontario CANADA
N6A 5B7
Ph. 519-661-2111 ext. 86780
Fax 519-661-3935



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 15:33:05 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 06 Jan 2005 13:38:10 -0800
Subject: [Microscopy] Re: Re: Looking for CdTe nannoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think formvar support film is not good for such type of experiment. You
need thin carbon film to reduce scatter from background and enhance
signal-to-noise ratio. I had difficulties to see nanogold particles (about
2 nm in diameter) in bright but dark field. In dark field on 1.8 nm carbon
they are perfectly visible. Your particles has lover density, so it would
be even trickier to see them than gold. Again, sometime it's easier to see
the particles on the film rather on the screen (so you focus on some junk
in hope to have your object nearby). Anyway, I think it's not such easy to
see nanosize object on the any support film. Negative staining would just
complicate the situation because UA staining for instance generated
approximately 0.8 nm granularity of background. I don't think you could
resolve 2 nm object well with 0.8 nm probe at least at the negative
staining condition. Sergey

At 07:12 AM 1/6/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Thu Jan 6 16:15:22 2005



From: hkonishi-at-indiana.edu
Date: Thu, 6 Jan 2005 16:57:07 -0500
Subject: [Microscopy] Gatan G1/ Epo-tek Epoxy 353ND

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello

I bought Epo-tek Epoxy 353ND since I heard Gatan G1 and 353ND are the same.
However, no instruction came with the epoxy. On the website I found that Cure
Time: 1 min.at 150°C or 5 min. at 100°C. For embedding materials, what
temperature and time is the best? Please advise.

Thank you,
Hiromi Konishi
Indiana University


From MicroscopyL-request-at-ns.microscopy.com Fri Jan 7 03:09:05 2005



From: Frank Eggert :      eggert-at-mikroanalytik.de
Date: Fri, 07 Jan 2005 10:14:44 +0100
Subject: [Microscopy] Re: Image grabbing systems for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Karen,

the best choice is an active system (with scan generator). You are going
to upgrade the electron microscope system to a digital SEM with all
benefits.

From my point of view one of the best systems (regarding the
performance and the costs) is:

http://www.pointelectronic.de/english/diss5e.htm
http://www.pointelectronic.de/english/links/links_sales.htm

.. I have no commercial interests or benefits with this product, only
detailed knowledge about the functionality (from my jobs in former times).

Best regards

Frank



Karen Bovard wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Jan 7 06:01:47 2005



From: Aarti Harle :      aarti_harle-at-yahoo.co.in
Date: Fri, 7 Jan 2005 04:06:48 -0800 (PST)
Subject: [Microscopy] Re: Re: Re: Looking for CdTe nannoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Its quit difficult to view the 2nm particles, as such
being very tiny and thin they will have a very little
contrast and it further depend on the atomic weight.

We have successfully viewd 2-3nm CdS particles on
formar film.
Yes you have to play with the aperature and
accelarting voltages and spot size.
According to my personal experience, though at 200kv
you will get the better resolution but for such kind I
maily use the accelarating voltage of 120kv which
gives better contrast.
Moreover the alignment should be perfect.
We use to align the Gun everytime and correct the
astigmata for such kind of samles
moreover one need absolute concentration and patience
for such kind of samples.
Many times I too use to get frusated with such kind of
samples but then slowly i started getting the good
results.
Pl do not hesistate to contact if u need any further
information
Goodluck

Regards
Arti


--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} I think formvar support film is not good for such
} type of experiment. You
} need thin carbon film to reduce scatter from
} background and enhance
} signal-to-noise ratio. I had difficulties to see
} nanogold particles (about
} 2 nm in diameter) in bright but dark field. In dark
} field on 1.8 nm carbon
} they are perfectly visible. Your particles has
} lover density, so it would
} be even trickier to see them than gold. Again,
} sometime it's easier to see
} the particles on the film rather on the screen (so
} you focus on some junk
} in hope to have your object nearby). Anyway, I think
} it's not such easy to
} see nanosize object on the any support film.
} Negative staining would just
} complicate the situation because UA staining for
} instance generated
} approximately 0.8 nm granularity of background. I
} don't think you could
} resolve 2 nm object well with 0.8 nm probe at least
} at the negative
} staining condition. Sergey
}
} At 07:12 AM 1/6/2005, you wrote:
}
}
}
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.msa.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -------------------------------------------------------------------------------
} }
} } Jonathon
} }
} } I know that individual 5nm gold spheres start to
} become difficult to
} } see in a real sample on carbon/formvar grids. This
} is not so much size
} } as the density you will get against a relatively
} thick background
} } coating (} 50nm). Cd and Te are both lighter than Au
} and if the
} } particles are not too symmetrical then I would have
} thought that they
} } would be practically invisible.
} }
} } It sounds like one of those classical e.m. problems
} where the
} } resolution of the microscope is not the issue but
} the resolution and
} } contrast of the sample may be. I suppose you could
} play around with the
} } voltage and aperture size to enhance contrast a
} bit, as well. Someone
} } has already mentioned shadowing but I wonder if a
} simple negative stain
} } might help - I really have no idea. But I'd be
} interested to hear how
} } you get on.
} }
} } It would certainly be worth trying with the bare
} particles if you can.
} }
} } Malcolm
} }
} } Malcolm Haswell
} } e.m. unit
} } School of Health, Natural and Social Sciences
} } University of Sunderland
} } Tyne & Wear
} } SR1 3SD
} } UK
} } e-mail: malcolm.haswell-at-sunderland.ac.uk
} }
} }
} }
} } ----- Original Message -----
} } } From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
} } Date: Wednesday, January 5, 2005 10:47 pm
} } Subject: [Microscopy] Looking for CdTe
} nannoparticles
} }
} } }
} } }
} } }
}
-------------------------------------------------------------------
} } } -----------
} } } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of
} } } AmericaTo Subscribe/Unsubscribe --
} } }
}
http://www.msa.microscopy.com/MicroscopyListserverOn-Line
} Help
} } }
}
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html--------
} } }
}
-------------------------------------------------------------------
} } } ----
} } }
} } } Hi:
} } }
} } } I need some expert advice so I can help a user
} in my lab. He wants
} } } to see
} } } some nannoparticles he has synthesized by the
} following procedure:
} } }
} } } } The sample is CdTe, a highly fluorescent NP
} with a shell of either
} } } } thioglycolic acid or 2-mercaptoethylamine. In
} theory they should
} } } be about
} } } } 2-5nm in diameter, but they are synthesized in
} aqueous solution,
} } } and in
} } } } order to properly seperate the particles I
} perform a ligand
} } } exchange and
} } } } redissolve into organic solvents(ie toluene).
} } }
} } } So, he shows up and I put his solution onto a
} carbon coated
} } } formvar grid. I
} } } look around. I don't see much, some junk, but
} nothing like
} } } nannoparticles.He is disappointed.
} } }
} } } I am scratching my head. Is there something
} there and I can't see
} } } it? Would
} } } I see it if it were there?
} } }
} } } Maybe you have some ideas?
} } }
} } } Would raw CdTe particles at 2 nm size have
} enough contrast to show up?
} } }
} } } Could the solution be so concentrated that it
} looks like a solid field
} } } rather than separate particles?
} } }
} } } The solution he gave me didn't really dry on the
} grid like I
} } } thought it
} } } would. How fast does toluene evaporate and could
} it mangle the
} } } formvar?
} } } Any other helpful hints to get some results?
} } }
} } } Thanks
} } }
} } }
} } } Jonathan Krupp
} } } Microscopy & Imaging Lab
} } } University of California
} } } Santa Cruz, CA 95064
} } } (831) 459-2477
} } } jmkrupp-at-cats.ucsc.edu
} } }
} } }
} } }
} } }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} 10833 Le Conte Ave, Room 33-080
} Los Angeles, CA 90095
}
} Phone: (310) 825-1144 (office)
} (310) 206-1029 (Lab)
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}
}


=====
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REGIONAL SOPHISTICATED INSTURMENTATION CENTER
INDIAN INSTITUTE OF TECHNOLOGY-BOMBAY
POWAI, MUMBAI - 400076. INDIA
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From MicroscopyL-request-at-ns.microscopy.com Fri Jan 7 12:00:25 2005



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 7 Jan 2005 12:44:33 -0500
Subject: [Microscopy] Gatan G1/ Epo-tek Epoxy 353ND

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The lower the temp, the longer the time. It will not cure at room temperature. However, if you go too hot, the stuff fumes a bit, so be careful. I use a hot plate at 120C, but I can't tell you the time since I use a small vise and it takes time for the vise to get up to temperature. There is a color change with this epoxy. I use a small dollop on a piece of Teflon that I can take off to tell me when it is done. The color goes from a clearish yellow to a deep brownish red. I would test it with your process. Use a sharp Exacta blade or a razor blade and poke it when you think that it looks done. When it is done, it will be hard.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)


-----Original Message-----
} From: hkonishi-at-indiana.edu [mailto:hkonishi-at-indiana.edu]
Sent: Thursday, January 06, 2005 4:57 PM
To: message to: MSA list

Hello

I bought Epo-tek Epoxy 353ND since I heard Gatan G1 and 353ND are the same.
However, no instruction came with the epoxy. On the website I found that Cure
Time: 1 min.at 150°C or 5 min. at 100°C. For embedding materials, what
temperature and time is the best? Please advise.

Thank you,
Hiromi Konishi
Indiana University



From MicroscopyL-request-at-ns.microscopy.com Fri Jan 7 12:24:18 2005



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 07 Jan 2005 12:29:30 -0600
Subject: [Microscopy] Medal analysis in NY area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I worked with a client earlier this week using SEM/EDS to characterize some
WW-II medals. The client wanted to determine the nature of authentic medals
so that forgeries might be easily detected.

I thought the session to be fairly straightforward. We looked at both the
paint and the metals involved and were able to clearly show a few things
that had never been seen before. That is, it appears that nobody had ever
looked at such items by SEM and EDS before. That seems strange to me, but I
guess there are still a lot of things that have not been examined.

Well, now I have been contacted by another collector from the NY area who
is wondering if labs are available there to do similar work. I have
forwarded his request below so that interested parties may contact him
directly. I suppose you may also contact me for more details about the work.

Warren

} } From popserve Wed Jan 5 13:39:02 2005
} Date: Wed, 5 Jan 2005 11:32:21 -0800 (PST)
} From: Marc Garlasco {marc-at-garlasco.com}
} Subject: [Microscopy] Tom Hansen
} To: wesaia-at-iastate.edu
} X-PMX-Version: 4.7.0.111621, Antispam-Engine: 2.0.0.0, Antispam-Data:
} 2005.1.5.1
} X-Perlmx-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0,
} __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_VERSION 0, __SANE_MSGID 0'
}
} Mr. Straszheim,
}
} I am a friend of Tom's and I am amazed at the
} cutting-edge work you guys did on the crosses
} yesterday! I would like to enlarge the pool of data
} by getting my crosses analyzed here in the NY area.
} Can you suggest good departments to contact?
}
} Regards,
} Marc Garlasco

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From MicroscopyL-request-at-ns.microscopy.com Fri Jan 7 12:47:20 2005



From: Ron Anderson :      randerson20-at-tampabay.rr.com
Date: Fri, 7 Jan 2005 13:52:25 -0500
Subject: [Microscopy] Microscopy Today January 2005 Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the January 2005 Microscopy Today table of contents. I will close
the subscription list for this issue on Tuesday 11 January 2005.

THIS ISSUE CONTAINS THE MT SALARY SURVEY RESULTS

Microscopists in North America and MSA members anywhere can subscribe for
free. Anyone else may subscribe for US$35 per year (to PARTIALLY cover
postage). All subscriptions at http://www.microscopy-today.com Thank you.

Unfolding and Folding Proteins
Stephen W. Carmichael, Mayo Clinic

Spiral Powder Overlays
P. Fraundorf and Shuhan Lin

TEM Morphometry Reveals Membrane Deficits in Parietal Cells Lacking Specific
Ion Transporters
Miller ML, Gawenis LR, Andringa A, Shull GE

Color Matching to Ink Jet Printers from a Computer Screen, Part 2
Jerry Sedgewick

Electron Tomography in the Study of Bacterial Structure and Function
Kenneth H. Downing,* Haixin Sui,* Luis R. Comolli* and Hoi-Ying Holman

Having Your Cake and Eating It Too: A Procedure for Obtaining Plan View and
Cross Section TEM Images from the Same Site
R.B. Irwin, A. Anciso, P.J. Jones, and C. Patton

Confocal Scanning Laser Holography: A Tool for Non-Invasive Internal
Measurement
RA McLeod, P Jacquemin, S Lai, RA Herring

Dehydration and Rehydration Issues in Biological Tissue Processing for
Electron Microscopy
Christian T. K.-H. Stadtländer

Microscopy Today 2004 Salary Survey Results
Ron Anderson and Barbara Foster

Funding Opportunities for Acquiring Major Equipment from Federal Granting
Agencies M&M 2004 Core Facility Management – Part I: NIH
Organizer: Debby Sherman

Specimen Capsules For Critical-Point Drying
Sol Sepsenwol

Industry News

NetNotes

Ron Anderson, Editor
Microscopy Today





From MicroscopyL-request-at-ns.microscopy.com Fri Jan 7 12:53:54 2005



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Fri, 7 Jan 2005 14:00:57 -0800
Subject: [Microscopy] Fwd: Looking for CdTe nannoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jon-
We have successfully imaged 1 nm particles under even more adverse
conditions. That is, in 70-nm thick sections of epoxy resin.

One has to collect two digital images and subtract them. The one is
run through a 3x3 kernel to smooth it, and the 1-nm gold image drops
out of that one.

I don't know whether you have digital image collection and
processing, but if you have, this would be an easy way to solve your
problem.


} X-Authentication-Warning: ns.microscopy.com: mail set sender to
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} Date: Wed, 5 Jan 2005 14:47:17 -0800
} To: microscopy-at-microscopy.com
} From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
} Subject: [Microscopy] Looking for CdTe nannoparticles
} X-UCSC-CATS-MailScanner: Found to be clean
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--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu
http://www.bgsu.edu/departments/biology/facilities/MnM
___________________________________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Fri Jan 7 15:37:29 2005



From: rebecca.burgin-at-onsemi.com (by way of MicroscopyListserver)
Date: Sat, 8 Jan 2005 07:36:54 -0600
Subject: [Microscopy] viaWWW:looking for a used SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hiromi,

As my experience shows it may be additional benefits in terms of strength is
you can increase a little bit humidity during the curing. Water vapour, for
example, placing an open bottle filled with water at some place closer to
your bonding. Hope it will help.

Victoria


----- Original Message -----
} From: "Walck, Scott D." {walck-at-ppg.com}
To: {hkonishi-at-indiana.edu}
Cc: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com}
Sent: Friday, January 07, 2005 9:44 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rebecca.burgin-at-onsemi.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, January 7, 2005 at 11:34:19
---------------------------------------------------------------------------

Email: rebecca.burgin-at-onsemi.com
Name: Rebecca Burgin

Organization: ON Semiconductor

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: We are in the market for a used Hitachi S4800/S4700 SEM (or comparable). Please contact me at 602-244-5775 (phone) or rebecca.burgin-at-onsemi.com (email)if you have one available or have any leads. Thanks in advance.



Rebecca

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Jan 8 08:58:25 2005



From: George Langford, Sc.D. :      amenex-at-amenex.com (by way of
Date: Sat, 8 Jan 2005 09:03:46 -0600
Subject: [Microscopy] How do I calculate a tubelength compensating lens ? LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Microscopists !

A while back I tried to locate a 21X BF/DF objective for an antique Bausch
& Lomb Research Metallograph that I am bringing back to life. So far, the
solution hasn't come into coincidence with my limited budget. However, I
am getting "close enough" by adapting a short-barrel Carl Zeiss 21X objective
which gives excellent images on the Kodak MDS 100 digital camera that I've
adapted to the metallograph.

However, the Zeiss objective was designed for a tube length of 190 mm, but
the metallograph uses a tube length of 215 mm. The images are good
enough that I really ought to accept the present situation. However, I am
nevertheless trying to do it right by adding a compensating lens in the light
path. However, I have not yet found out how to do the math. I understand
that a negative lens is needed to stretch the image distance from 190mm to
215mm. I even have the Bausch & Lomb compensating lens that comes with
their vertical-illuminator attachment for transmitted-light microscopes, but
that lens is designed to shift the image distance by 55mm, i.e., the extra
optical path length introduced by the vertical illuminator.

Can anyone steer me to a suitable on-line or library reference that shows
how to do the calculation ?


From MicroscopyL-request-at-ns.microscopy.com Sat Jan 8 19:38:41 2005



From: Bob Sunley :      rosunley-at-shaw.ca
Date: Sat, 08 Jan 2005 19:46:23 -0600
Subject: [Microscopy] Re: How do I calculate a tubelength compensating lens ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On 8 Jan 2005, at 9:03, George Langford, Sc.D. wrote:
}
} Hello Microscopists !
}
} A while back I tried to locate a 21X BF/DF objective for an antique Bausch &
} Lomb Research Metallograph that I am bringing back to life. So far, the
} solution hasn't come into coincidence with my limited budget. However, I am
} getting "close enough" by adapting a short-barrel Carl Zeiss 21X objective
} which gives excellent images on the Kodak MDS 100 digital camera that I've
} adapted to the metallograph.
}
} However, the Zeiss objective was designed for a tube length of 190 mm, but the
} metallograph uses a tube length of 215 mm. The images are good enough that I
} really ought to accept the present situation. However, I am nevertheless
} trying to do it right by adding a compensating lens in the light path.
} However, I have not yet found out how to do the math. I understand that a
} negative lens is needed to stretch the image distance from 190mm to 215mm. I
} even have the Bausch & Lomb compensating lens that comes with their
} vertical-illuminator attachment for transmitted-light microscopes, but that
} lens is designed to shift the image distance by 55mm, i.e., the extra optical
} path length introduced by the vertical illuminator.
}
} Can anyone steer me to a suitable on-line or library reference that shows
} how to do the calculation ?

Sorry, can't help you with the math, but the following link will show the tolerance of
dry objectives to changes in tube length based on the n.a of the lens.

http://f5.grp.yahoofs.com/v1/EILgQXu2eDH9tzkIFZ29JBzTdWL8PPKzbGdV05LJ_UN
VpTr3-X8gRRhMZAwxybzrOrHorfSEvpxBT-
63eDW6F4wGig/Microscope%20Theory%20and%20Figures/Tube%20length%20devi
ation.JPG

or go to http://groups.yahoo.com/group/Microscope, on the left side of the page,
click on Files, scroll down to folder named "Microscope Theory and Figures"
click and then click on Tube length deviation.jpg

Unless your objective has an n.a. } 0.5 you won't introduce any measurable
distortion in the image.
You might have to piece the long link back together in your browser.

Bob Sunley


From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 03:56:31 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 10 Jan 2005 05:04:46 -0500
Subject: [Microscopy] SEM: Digital Image System for SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Karen Bovard wrote:
=======================================================================
I have a JEOL 840A SEM and am interested in upgrading it to digital
photograpy capabilities.

I am aware of the Orion, SIS ADDA II, and the JEOL Orion systems.

Are there any different options (preferably cheaper) to consider?

Karen Bovard
EM Lab
Pathology
Creighton University
Omaha, NE
==========================================================================
The "cheapest" option, which was offered by SPI Supplies for many years was
Spectrum Mono (previously known in some parts of the world as Image Slave).
However it is no longer being offered by the manufacturer.

We are now offering the ORION™ Digital Image System for SEMs, or in simple
words, the Orion "frame grabber". See URL
http://www.2spi.
com/catalog/instruments/ORION_Digital_Image_Acquisition_System.html
Various optional modules are also available to extanding the software's
capabilities.

It is a passive image capture system, is easy to operate and easy to install
We use it all the time in our own laboratory and have found it to be
quite easy to learn to use as well. An active image capture system it is
not, but then again, there are many who do not need the benefits of an
active capture system (which is much more expensive as well).

This system should not be confused with the JEOL Orion system. It is
unfortunate that two firms are using the same trade name since it is bound
to cause confusion in the marketplace. These are most certainly not the
same product.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
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From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 08:08:16 2005



From: diller-at-stefan-diller.com (by way of MicroscopyListserver)
Date: Mon, 10 Jan 2005 08:16:53 -0600
Subject: [Microscopy] viaWWW: SEM on lubricates or grease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-stefan-diller.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, January 10, 2005 at 03:22:34
---------------------------------------------------------------------------

Email: diller-at-stefan-diller.com
Name: Stefan Diller

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello,
does anyone out there have experience in SEM on lubricates or grease?
I need to do SEM on nanoparticles in lubricates as well as various states of lubricates growing old...
My first idea is using a coolstage, but is there any possiblity (without a low vac SEM) to work around this not having one?

Thanks for your help.
Stefan Diller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 10:12:09 2005



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 10 Jan 2005 16:20:51 +0000 (GMT Standard Time)
Subject: [Microscopy] Re: viaWWW: SEM on lubricates or grease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stefan,

Try www.quantomix.com

} From their Web site:
'QuantomiX develops and commercializes breakthrough
solutions based on its proprietary wetSEM™ technology which
enables direct scanning electron microscopy (SEM) of wet
samples.'

I have no experience of using the holders or any connection
with the company. I've just seen them advertising.

Ron

On Mon, 10 Jan 2005 08:16:53 -0600 by way of
MicroscopyListserver {diller-at-stefan-diller.com} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
Society of America } To Subscribe/Unsubscribe --
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}
} Below is the result of your feedback form
(NJZFM-ultra-55). It was submitted by
(diller-at-stefan-diller.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html
on Monday, January 10, 2005 at 03:22:34 }
---------------------------------------------------------------------------
}
} Email: diller-at-stefan-diller.com } Name: Stefan Diller } }
Title-Subject: [Microscopy] [Filtered] MListserver: } }
Question: Hello, } does anyone out there have experience in
SEM on lubricates or grease? } I need to do SEM on
nanoparticles in lubricates as well as various states of
lubricates growing old... } My first idea is using a
coolstage, but is there any possiblity (without a low vac
SEM) to work around this not having one? } } Thanks for
your help. } Stefan Diller } }
---------------------------------------------------------------------------
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk
http://www-em.materials.ox.ac.uk/
*********************************





From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 11:50:28 2005



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 10 Jan 2005 09:58:12 -0800 (PST)
Subject: [Microscopy] Re: viaWWW: SEM on lubricates or grease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think you may need to do frozen imaging or cryoSEM. It might work doing
a freeze-fracture-etch and then image with a high resolution coating.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Mon, 10 Jan 2005, by way of MicroscopyListserver wrote:

}
}
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} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-stefan-diller.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, January 10, 2005 at 03:22:34
} ---------------------------------------------------------------------------
}
} Email: diller-at-stefan-diller.com
} Name: Stefan Diller
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: Hello,
} does anyone out there have experience in SEM on lubricates or grease?
} I need to do SEM on nanoparticles in lubricates as well as various states of lubricates growing old...
} My first idea is using a coolstage, but is there any possiblity (without a low vac SEM) to work around this not having one?
}
} Thanks for your help.
} Stefan Diller
}
} ---------------------------------------------------------------------------
}


From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 12:18:15 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Mon, 10 Jan 2005 10:26:05 -0800 (PST)
Subject: [Microscopy] Re: SEM on lubricants or grease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stefan:

I have experience looking at greases and lubricants in
the SEM (JEOL JSM-5800LV). One such request had
nanoparticles in the grease. Unfortunately, my
results aren't promising.

I don't really see how a lubricant can be imaged
without using an environmental chamber SEM. I had
limited success when the lubricant thinned out enough
to where I can begin to see the nanoparticles. It
also helped that the particles were tungsten-based for
backscatter image contrast. Resolution was poor. EDS
analysis was somewhat doable.

Can you perhaps separate or concentrate the particles
by using solvent and centrifuge techniques before
imaging? This may help.

Stu Smalinskas, P.E.
SKF USA
Plymouth, Michigan
(734) 414-6862

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Stefan wrote:

Question: Hello,
does anyone out there have experience in SEM on
lubricates or grease?
I need to do SEM on nanoparticles in lubricates as
well as various states of lubricates growing old...
My first idea is using a coolstage, but is there any
possiblity (without a low vac SEM) to work around this
not having one?

Thanks for your help.
Stefan Diller

Email: diller-at-stefan-diller.com
Name: Stefan Diller



__________________________________
Do you Yahoo!?
Yahoo! Mail - Find what you need with new enhanced search.
http://info.mail.yahoo.com/mail_250


From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 12:41:44 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Mon, 10 Jan 2005 12:23:26 -0800
Subject: [Microscopy] viaWWW: SEM on lubricates or grease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stefan

In do have a cheap and cheerful way to overcome your problem. One feature
however may make my explanation null and void! You need a microscope that
has a manifold directly pumped by a diffusion or turbo pump, not a manifold
that bleeds the specimen vacuum to the gun? You also need a backscattered
electron detector. In this procedure we are taking advantage of a poor
vacuum bleeding away surface charge and reducing media evaporation from the
specimen.

If you have a manifold system and a backscattered detector the following
works very well for up to 20 minute working periods. Take a rubber
bung/stopper, that will fit into the pumping line at the rear of the
specimen chamber, freeze it in liquid nitrogen and drill a 0.5mm hole in the
bung/stopper. Prepare your specimen and place it in to the microscope at
the same time fitting the bung/stopper in to the pumping line. Pump down
and switch off the SE detector bringing the BSE detector into play. Use the
BSE detector to observe the specimen in the "poor vacuum" environment that
you have created. I have used this technique many times, the only drawback
is that the many manufacturers took us away from a decent vacuum system when
they decided to pump the column through the specimen chamber, rather than
pumping the microscope through a manifold.

So users of older microscopes have the cheapest possible "VP System", but
those caught in the middle era in SEM development miss out on this one I am
afraid!

Good luck

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
www.emcourses.com tel +44 1280 816512 fax +44 1280 814007

PS I probably should not point out that we once used this system to watch
paint dry!


----- Original Message -----
} From: "by way of MicroscopyListserver" {diller-at-stefan-diller.com}
To: {microscopy-at-microscopy.com}
Sent: Monday, January 10, 2005 2:16 PM

One of my colleagues many years ago at Lockheed, George Hopple, had
excellent success critical point drying greases. He was then able to
image the thickener and the oil, and the quite striking differences in
the greases were successfully tied to bearing failures in gyroscopes. I
don't have any contact information for George at this time, as he left
Lockheed over a decade ago, but maybe somebody out there can find him.

John Mardinly
Intel


-----Original Message-----
} From: by way of MicroscopyListserver [mailto:diller-at-stefan-diller.com]
Sent: Monday, January 10, 2005 6:17 AM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (diller-at-stefan-diller.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
January 10, 2005 at 03:22:34
------------------------------------------------------------------------
---

Email: diller-at-stefan-diller.com
Name: Stefan Diller

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello,
does anyone out there have experience in SEM on lubricates or grease?
I need to do SEM on nanoparticles in lubricates as well as various
states of lubricates growing old...
My first idea is using a coolstage, but is there any possiblity (without
a low vac SEM) to work around this not having one?

Thanks for your help.
Stefan Diller

------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 18:02:54 2005



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 10 Jan 2005 16:11:14 -0800 (PST)
Subject: [Microscopy] inverted microscope imaging algae

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I was reading over some things about diatoms and wanted to get a good
light microscope image of some organisms from a biofilm/water. I was
wondering what is a good way of imaging live microbes with an inverted
light microscope? Just use a coverslip, slide and place it upside down on
the inverted stage? I read about using modified petrie dishes too.
Any advice appreciated.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793


From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 20:22:07 2005



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 10 Jan 2005 16:11:14 -0800 (PST)
Subject: [Microscopy] inverted microscope imaging algae

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
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Hello,
I was reading over some things about diatoms and wanted to get a good
light microscope image of some organisms from a biofilm/water. I was
wondering what is a good way of imaging live microbes with an inverted
light microscope? Just use a coverslip, slide and place it upside down on
the inverted stage? I read about using modified petrie dishes too.
Any advice appreciated.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 20:38:34 2005



From: diller-at-stefan-diller.com (by way of MicroscopyListserver)
Date: Mon, 10 Jan 2005 08:16:53 -0600
Subject: [Microscopy] viaWWW: SEM on lubricates or grease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-stefan-diller.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, January 10, 2005 at 03:22:34
---------------------------------------------------------------------------

Email: diller-at-stefan-diller.com
Name: Stefan Diller

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello,
does anyone out there have experience in SEM on lubricates or grease?
I need to do SEM on nanoparticles in lubricates as well as various states of lubricates growing old...
My first idea is using a coolstage, but is there any possiblity (without a low vac SEM) to work around this not having one?

Thanks for your help.
Stefan Diller

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 20:39:09 2005



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 10 Jan 2005 09:58:12 -0800 (PST)
Subject: [Microscopy] Re: viaWWW: SEM on lubricates or grease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
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I think you may need to do frozen imaging or cryoSEM. It might work doing
a freeze-fracture-etch and then image with a high resolution coating.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Mon, 10 Jan 2005, by way of MicroscopyListserver wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-stefan-diller.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, January 10, 2005 at 03:22:34
} ---------------------------------------------------------------------------
}
} Email: diller-at-stefan-diller.com
} Name: Stefan Diller
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: Hello,
} does anyone out there have experience in SEM on lubricates or grease?
} I need to do SEM on nanoparticles in lubricates as well as various states of lubricates growing old...
} My first idea is using a coolstage, but is there any possiblity (without a low vac SEM) to work around this not having one?
}
} Thanks for your help.
} Stefan Diller
}
} ---------------------------------------------------------------------------
}



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 20:40:41 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 10 Jan 2005 05:04:46 -0500
Subject: [Microscopy] SEM: Digital Image System for SEMs

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------------------------------------------------------------------------------
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Karen Bovard wrote:
=======================================================================
I have a JEOL 840A SEM and am interested in upgrading it to digital
photograpy capabilities.

I am aware of the Orion, SIS ADDA II, and the JEOL Orion systems.

Are there any different options (preferably cheaper) to consider?

Karen Bovard
EM Lab
Pathology
Creighton University
Omaha, NE
==========================================================================
The "cheapest" option, which was offered by SPI Supplies for many years was
Spectrum Mono (previously known in some parts of the world as Image Slave).
However it is no longer being offered by the manufacturer.

We are now offering the ORION™ Digital Image System for SEMs, or in simple
words, the Orion "frame grabber". See URL
http://www.2spi.
com/catalog/instruments/ORION_Digital_Image_Acquisition_System.html
Various optional modules are also available to extanding the software's
capabilities.

It is a passive image capture system, is easy to operate and easy to install
We use it all the time in our own laboratory and have found it to be
quite easy to learn to use as well. An active image capture system it is
not, but then again, there are many who do not need the benefits of an
active capture system (which is much more expensive as well).

This system should not be confused with the JEOL Orion system. It is
unfortunate that two firms are using the same trade name since it is bound
to cause confusion in the marketplace. These are most certainly not the
same product.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================






From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 20:46:08 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Mon, 10 Jan 2005 10:26:05 -0800 (PST)
Subject: [Microscopy] Re: SEM on lubricants or grease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Stefan:

I have experience looking at greases and lubricants in
the SEM (JEOL JSM-5800LV). One such request had
nanoparticles in the grease. Unfortunately, my
results aren't promising.

I don't really see how a lubricant can be imaged
without using an environmental chamber SEM. I had
limited success when the lubricant thinned out enough
to where I can begin to see the nanoparticles. It
also helped that the particles were tungsten-based for
backscatter image contrast. Resolution was poor. EDS
analysis was somewhat doable.

Can you perhaps separate or concentrate the particles
by using solvent and centrifuge techniques before
imaging? This may help.

Stu Smalinskas, P.E.
SKF USA
Plymouth, Michigan
(734) 414-6862

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Stefan wrote:

Question: Hello,
does anyone out there have experience in SEM on
lubricates or grease?
I need to do SEM on nanoparticles in lubricates as
well as various states of lubricates growing old...
My first idea is using a coolstage, but is there any
possiblity (without a low vac SEM) to work around this
not having one?

Thanks for your help.
Stefan Diller

Email: diller-at-stefan-diller.com
Name: Stefan Diller



__________________________________
Do you Yahoo!?
Yahoo! Mail - Find what you need with new enhanced search.
http://info.mail.yahoo.com/mail_250



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 10 20:46:35 2005



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 10 Jan 2005 16:20:51 +0000 (GMT Standard Time)
Subject: [Microscopy] Re: viaWWW: SEM on lubricates or grease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



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Stefan,

Try www.quantomix.com

} From their Web site:
'QuantomiX develops and commercializes breakthrough
solutions based on its proprietary wetSEM™ technology which
enables direct scanning electron microscopy (SEM) of wet
samples.'

I have no experience of using the holders or any connection
with the company. I've just seen them advertising.

Ron

On Mon, 10 Jan 2005 08:16:53 -0600 by way of
MicroscopyListserver {diller-at-stefan-diller.com} wrote:

}
}
}
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Society of America } To Subscribe/Unsubscribe --
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On-Line Help
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}
-------------------------------------------------------------------------------
}
} Below is the result of your feedback form
(NJZFM-ultra-55). It was submitted by
(diller-at-stefan-diller.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html
on Monday, January 10, 2005 at 03:22:34 }
---------------------------------------------------------------------------
}
} Email: diller-at-stefan-diller.com } Name: Stefan Diller } }
Title-Subject: [Microscopy] [Filtered] MListserver: } }
Question: Hello, } does anyone out there have experience in
SEM on lubricates or grease? } I need to do SEM on
nanoparticles in lubricates as well as various states of
lubricates growing old... } My first idea is using a
coolstage, but is there any possiblity (without a low vac
SEM) to work around this not having one? } } Thanks for
your help. } Stefan Diller } }
---------------------------------------------------------------------------
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk
http://www-em.materials.ox.ac.uk/
*********************************






From MicroscopyL-request-at-ns.microscopy.com Tue Jan 11 08:35:17 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Tue, 11 Jan 2005 08:47:20 -0600
Subject: [Microscopy] Administrivia: Nestor is testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Testing the server links. The access file is no longer writable.

Please ignore this message

Nestor


From MicroscopyL-request-at-ns.microscopy.com Tue Jan 11 08:50:48 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Tue, 11 Jan 2005 08:59:44 -0600
Subject: [Microscopy] Administrivia: Possible duplicate Emails

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues...

Some of you may be experiencing duplicate Email deliveries.
It is not obvious why this is happening. I am looking into the problem.


Nestor
Your Friendly Neighborhood SysOp.



From MicroscopyL-request-at-ns.microscopy.com Tue Jan 11 15:12:22 2005



From: Lehman, Ann R :      Ann.Lehman-at-trincoll.edu
Date: Tue, 11 Jan 2005 16:13:01 -0500
Subject: [Microscopy] EM400 OL current

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

A colleague has a Philips EM400T TEM that is exhibiting odd behavior as
the user mags up and down. At certain mags, the OL current goes way out,
but to a reproducible figure, as follows:

At 100kV with Z-axis corrected, the OL current at true focus reads 6.66.
When tracking from the upper mags toward the lower, at the transition
between 130kX to 100kX, the OL current jumps to 6.44. If the user
corrects back to true focus (again with an OL reading of 6.66 or 6.67),
then tracking down to the next mag step causes the OL to jump again back
to 6.44. This jump proceeds at every mag until the user reaches 2800X,
when the OL goes to (or stays at) 6.66.

Also confusing, this sequence doesn't happen 100% of the time, and not
always at every mag within the mag range described, but it does so more
often than not. My guess is that a certain board that defines lens
correlations in the middle-mag range has a fluky relay. Does anyone have
an idea which board(s) we should be looking at to test this theory? Or
does anyone have a better idea on how to address this issue?

We do have access to another EM400 that we can use for parts. Any help
on how to proceed would be very helpful, and gratefully acknowledged!

Thanks all,

Ann Hein Lehman
Assistant Director, Electron Microscopy Facility
Trinity College
300 Summit Street
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman





From MicroscopyL-request-at-ns.microscopy.com Tue Jan 11 16:07:05 2005



From: K.N. Bozhilov :      bozhilov-at-ucr.edu
Date: Tue, 11 Jan 2005 16:42:37 -0800
Subject: [Microscopy] Depth of focus

Contents Retrieved from Microscopy Listserver Archives
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Hello Karen,

Just a piece of information to help with your decision (hopefully not to
confuse you):

A PASSIVE system such as the one Mary mentions essentially takes the data
generated by the SEM and digitizes them. Basically, you work with your SEM
and when you see an image that you want to record, you push a button and the
PC records the image. This also means, that you are limited to what the SEM
can provide. If the SEM scan generator can only provide a 1000 line image,
the highest resolution will be something like 1300x1000. On the other hand,
passive systems can be a bit less expensive.

An ACTIVE system basically replaces the scan generator with one that is
controllable by the PC. Again, you would work with your SEM normally until
you see an image that you want to record. You then push a button, and the PC
records an image. Different from the passive system, though, the scanning is
now controlled by the PC and you can acquire the images at a higher
resolution (up to 4ooox4ooo in case of our ADDA). An active system also
provides control over dwell-time (noise reduction) and synchronization with
60Hz noise signals, generally resulting in better images. Furthermore, with
additional hardware you can also easily acquire elemental distribution maps.
An active system is usually a bit more expensive.

Disclaimer: We produce and sell the ADDA II system mentioned in Karen's
original email, which is both passive and active.


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Thursday, January 06, 2005 10:18
To: Microscopy


----- Original Message -----
} From: "Mary Mager" {mager-at-interchange.ubc.ca}
To: "Karen Bovard" {kbovard-at-creighton.edu}
Sent: Thursday, January 06, 2005 9:15 AM

According to the established TEM literature the depth of focus is:

Dfocus = Dfield x Magnification x Magnification.

With depth of field around 50 nm, which is typical imaging condition,
according to the above relation the depth of focus should be about 500
meters at magnification of 100,000.

We have a CCD camera and a TV camera mounted beneath the CCD on one of
our TEMs. Well, the image focused on the TV is not in perfect focus on
the CCD and vice versa at magnifications in the range of 100,000 and
above.

What is wrong or is there something I am missing in the Depth of focus
equations?

Krassimir N. Bozhilov



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 12 02:55:16 2005



From: Michel Ribardiere :      m.ribardiere-at-jeol.fr
Date: Wed, 12 Jan 2005 10:00:32 +0100
Subject: [Microscopy] RE: Depth of focus

Contents Retrieved from Microscopy Listserver Archives
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dear Krassimir,

I feel that one of the 2 cameras is not well focused
Find the focus of the image on fluorescent screen and compare with both
cameras; you will find the one in fault
Usually the CCDs cameras have a focus adjustment. Refer to your provider for
the procedure
The focus should be same on all cameras and fluo screen. the depth is always
enough to get good focus on axial cameras.

The only cameras which usually needs to change focus are those mounted after
an EELS system.
best regards
Mic





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--
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

According to the established TEM literature the depth of focus is:

Dfocus = Dfield x Magnification x Magnification.

With depth of field around 50 nm, which is typical imaging condition,
according to the above relation the depth of focus should be about 500
meters at magnification of 100,000.

We have a CCD camera and a TV camera mounted beneath the CCD on one of
our TEMs. Well, the image focused on the TV is not in perfect focus on
the CCD and vice versa at magnifications in the range of 100,000 and
above.

What is wrong or is there something I am missing in the Depth of focus
equations?

Krassimir N. Bozhilov



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 12 09:22:05 2005



From: Rhonda Stroud :      stroud-at-nrl.navy.mil
Date: Wed, 12 Jan 2005 10:21:35 -0500
Subject: [Microscopy] TEM: ion mill comparisons

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm looking for user feedback on low angle ion mills.
Please email me directly with your experience on
reliability and relative advantages of the different
vendors' models.

Thanks,

Rhonda




From MicroscopyL-request-at-ns.microscopy.com Wed Jan 12 09:39:40 2005



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Wed, 12 Jan 2005 11:39:14 -0400
Subject: [Microscopy] Etec Autoscan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I'm looking at a paper that did some work with and Etec Autoscan. I'm not
very familiar with the instrument, and no model number is given. Can anybody
fill me a bit on the history of this SEM? What is it's vintage, W, Lab6
or FE, etc.?
Does the company exist under another name? All the images and info I can
find
on the web look to be a 70s to 80s type instrument, but...

Gee, a "History of Microscope Manufacturers: Intrigue, Bankruptcy and
Hostile Takeovers"
web site would be handy...

As usual, thanks in advance!

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 12 14:59:33 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Wed, 12 Jan 2005 15:59:08 -0500
Subject: [Microscopy] Replacement lamp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for a source for replacement lamps for an ancient Leitz
Orthoplan microscope (ca. 1965 I think). I would appreciate any hints as
to where I might purchase such.
Thanks, Greg


Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 12 16:05:23 2005



From: White, Woody N. :      nwwhite-at-bwxt.com
Date: Wed, 12 Jan 2005 15:56:11 -0600
Subject: [Microscopy] Replacement lamp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greg,

I don't know the bulb number, but you might try:
www.bulbdirect.com

At least they carry some Leitz bulbs...

Woody



-----Original Message-----
} From: Greg Erdos [mailto:gwe-at-ufl.edu]
Sent: Wednesday, January 12, 2005 3:59 PM
To: Microscopy-at-MSA.Microscopy.Com

I am looking for a source for replacement lamps for an ancient Leitz
Orthoplan microscope (ca. 1965 I think). I would appreciate any hints as
to where I might purchase such.
Thanks, Greg


Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 12 16:51:29 2005



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Wed, 12 Jan 2005 22:29:15 +0000
Subject: [Microscopy] Re: Depth of focus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Can you actually focus the image on both cameras?

If either or both of the cameras is lens coupled, then there may be a
problem with the focusing of the camera itself on the scintillator.

Having said that, yes, I think that there can often be small focus
differences between cameras and phosphors at different levels.,
especially between a wide-filed CCD mounted above the viewing chamber
and a high mag CCD beneath the viewing chamber.
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 03:07:54 2005



From: Gordon Couger :      gcc-at-couger.com
Date: Thu, 13 Jan 2005 03:07:33 -0600
Subject: [Microscopy] Help on Goerz 3D Condenser

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I have a C. P. Goerz 3D Condenser for a microscope labeled Goerz
MOM Hungary in a box marked Goerz American Optical Company, New
York and a tag Universal had written and stuck on the box with a
hand written number 1060 on it.

I can find no reference at all to it on the Internet. It appears
to be manufactured in the Post war years from the look of the
labels, box, materials and workmanship. So I suspect it was made
in the 50's or 60's in Hungary and imported by American Optical.

It is fully working and in fine working condition and I would
like to know any thing you have about it. Of course a copy of
operating manual would be my ultimate objective.

Thanks
Gordon
Gordon Couger

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 08:24:07 2005



From: mingram-at-rodel.com (by way of MicroscopyListserver)
Date: Thu, 13 Jan 2005 08:24:03 -0600
Subject: [Microscopy] viaWWW: Third Party Support for LEICA Ergoplan microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mingram-at-rodel.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, January 13, 2005 at 07:18:54
---------------------------------------------------------------------------

Email: mingram-at-rodel.com
Name: Michael Ingram

Organization: Rohm and Haas Electonic Materials

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: All,

I have two LEICA Ergoplan defect review microscopes configured for 200 mm wafers. I am looking for third party service and support. The OEM charges are too high.

Both systems are running VisconNT defect review software. One is a manual load system and the second has a wafer handling robot attached. The manual load systems is having some startup errors, which might be eprom errors. The auto load systems has just been moved to my lab, and I am looking for someone to come in to install and check out the system. I can supply pictures for both tools.

Does any one know of third party support for these type of tools.



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 08:34:54 2005



From: jwilkinson-at-seton.org (by way of Ask-A-Microscopist)
Date: Thu, 13 Jan 2005 08:34:34 -0600
Subject: [Microscopy] AskAMicroscopist: Leica specimen trimmer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jwilkinson-at-seton.org) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, January 12, 2005 at 09:45:18
---------------------------------------------------------------------------

Email: jwilkinson-at-seton.org
Name: Joyce Wilkinson

Organization: Brackenridge Hospital

Education: Graduate College

Location: Austin, Texas USA

Question: The EM lab at Brackenridge in Austin Texas is considering a purchase of Leica specimen trimmer. I would appreciate your opinion of the valus of the trimmer. I have hear Pro's and con's and would value your experience.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 11:03:30 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Thu, 13 Jan 2005 12:03:04 -0500
Subject: [Microscopy] Leitz bulb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to every one who responded to my query about a replacement bulb. I
found one at donsbulbs.com. I am going to check out all of the other
suggested sources as Don does not accept credit cards.

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 11:32:41 2005



From: Peter Ingram :      p.ingram-at-cellbio.duke.edu
Date: Thu, 13 Jan 2005 13:46:27 -0500
Subject: [Microscopy] Re: Etec Autoscan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear James,
I operated an ETEC Autoscan (Manufacturer: "ETEC", model: "Autoscan") that
was purchased in 1973, before I started here. It was renowned for beautiful
images, very low mag capability and was a favourite SEM of its time. I
believe many of David Scharf's brilliant poster images are recorded on an
modified ETEC Autoscan. The Perkin-Elmer company bought ETEC up in the early
'80s for their electron-beam lithography system and shut down the SEM
division. There are still some running, including my old one that I sold to
the military. To answer your questions: it was a conventional W filament
SEM, mag. 5 to 200,000X. No FE, no VP-SEM, no computer, etc., just a good,
solid SEM with top resolution about 6.0 nm. They were good for their day,
but a small company that maybe couldn't keep up when more companies started
to manufacture SEMs.
I'm sure others know more, but no one seemed to be answering.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca

----- Original Message -----
} From: "James M. Ehrman" {jehrman-at-mta.ca}
To: "Microscopy Listserv" {Microscopy-at-MSA.Microscopy.com}
Sent: Wednesday, January 12, 2005 7:39 AM

Hey Jim!

Yes. I have owned directly or indirectly about 4 of these
instruments over the past 30+ years. They were conceived in about
1969 by a company in California (ETEC Corp) and were the first
serious challenge to the Cambridge Stereoscan and JEOL U2. They were
considered the "Rolls Royce" of SEMs in their day until Jim Dow (I
think that was his name) sold the company in the early 1980s. I
believe Perkin-Elmer purchased it in order to make the first electron
beam lithography devices The company (ETEC) still exists today as
far as I am aware for this purpose. They no longer make SEMs.

It was innovative for its time in that it was of a completely
modular design where whole functionalities could be exhanged
overnight as nim-bin modules. As an SEM it only used W filaments.

Also, as far as I know, there are still a fair number of
operating models out there. All mine I am afraid have been replaced
and/or donated but one I gave to Puerto Rico was working (with a lot
of TLC) until about 2 years ago. FYI, there is a person who has a
company who deals in spares and rebuilds ETECs - his name is Gary
Easton and his company is "Scanners Company" in Maryland somewhere
(Google to find out!) Also another fellow is Hank Bebe who works for
the Rich Lee Group who knows as much as anyone about the Autoscan.

I haven't checked Google to verify my memory of all the above
but I don't believe I am far off!

Please feel free to give me a call if you would like to
discuss more. There are some fun anecdotes concerning this
instrument and the ETEC company!

Cheers etc

Peter



} -
}
} Hi all,
}
} I'm looking at a paper that did some work with and Etec Autoscan. I'm not
} very familiar with the instrument, and no model number is given. Can anybody
} fill me a bit on the history of this SEM? What is it's vintage, W,
} Lab6 or FE, etc.?
} Does the company exist under another name? All the images and info I can find
} on the web look to be a 70s to 80s type instrument, but...
}
} Gee, a "History of Microscope Manufacturers: Intrigue, Bankruptcy
} and Hostile Takeovers"
} web site would be handy...
}
} As usual, thanks in advance!
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/dmf


--
Peter Ingram
Sr. Physicist
Adj. Professor of Pathology,
Duke University Medical Center
Box 90319
LaSalle Street Extension
DURHAM NC USA 27708-0319

Tel: (919) 660-2695
Fax: (919) 660-2671
e-mail: p.ingram-at-cellbio.duke.edu
http://152.3.54.136/AEM_LAB.html


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 13:15:50 2005



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Thu, 13 Jan 2005 15:15:21 -0400
Subject: [Microscopy] Etec Autoscan - thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Thanks to all who sent the many detailed descriptions of the Etec
Autoscan. I have more
than enough information for what I was curious about. But it's always
good to read about
scopes that people have known and loved. This looks to be true for the
Autoscan. I think the
subject has come up before, but it would be nice to have a repository of
images and specifications
for the various instruments through the ages. Oops! Sounds like I just
volunteered. But if those
interested would like to forward me info about their favorite (or most
dreaded) scope and interesting
anecdotes, horror stories, etc. I'll see what I can do about putting a
"Rogue's Gallery" on my
website. Any interest out there?

Thanks again,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 13:24:17 2005



From: Ephram Shizgal :      shizgal-at-lv-em.com
Date: Thu, 13 Jan 2005 14:21:45 -0500
Subject: [Microscopy] LKB Ultramicrotome III

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Would anyone know around when the LKB Ultramicrotome III was introduced ?

Thanks,


Ephram Shizgal
LVEM Technology Team

Delong Instruments/Delong America
In USA: 1-866-DELONGUSA (1-866-335-6648)
International: +514-904-1202
www.lv-em.com
info-at-lv-em.com




From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 14:09:18 2005



From: Fred A Hayes :      fahayes-at-comcast.net
Date: Thu, 13 Jan 2005 15:08:48 -0500
Subject: [Microscopy] used microtome and knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking to buy a used cryo ultramicrotome and glass knife maker that
work. Please respond off line.

Fred A Hayes
Ann Arbor MI
734-996-2012



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 14:19:15 2005



From: Allen Sampson :      ars-at-sem.com
Date: Thu, 13 Jan 2005 14:20:03 -0600
Subject: [Microscopy] RE: Etec Autoscan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The website idea isn't bad, but it would be very frustrating to put
together, much less maintain. I'd host it, though, if anyone wants to
help.

ETEC is a company that grew in the 70's, started by some engineers from MAC
(you can still see some MAC microprobes around). They were very successful
and made some solid, well engineered SEMs. One of the more interesting
aspects is the wide range of accessories they had available. These
instruments were extreme research machines and anything they could think of
making for it, they did. There was another model, the Omniscan, that had
some cute ideas but ended up being a maintenance nightmare. For the most
part, they used tungsten cathode, although LaB6 became available around the
end (the first to offer it that I know, the tips didn't last long, though).
They were also developing a variable pressure SEM that never made it to
market.

The end, by the way, was around 1983. ETEC had been developing Electron
Beam Lithography (EBL) for years. In 1979, they were bought by
Perkin-Elmer, who wanted the EBL component, but didn't care about the
laboratory instruments. They simply let the SEM manufacturing die a slow
death, selling off existing inventory, without ever really letting
customers know.

The ETEC EBL was a raster-based device. An elaborate table supported by
air bearings held the wafer, which was stepped around to allow the beam to
expose each small square area of the wafer. Since it had to store the
pattern for an entire wafer at a resolution of less than 100nm, large
amounts of memory were needed. Shortly after being bought by Perkin-Elmer,
some Japanese manufacturer's came in with vector based EBL systems and
pretty well trounced ETEC.

ETEC lives on today as an independent (I think) manufacturer of EBL.

I left ETEC in 1982. It was becoming obvious that they were winding down
the SEM business and trying to pressure me into the EBL lines. They
virtually sold you a service engineer along with the instrument - he'd move
where the instrument went and be on 24/7 call. Now, some SEM customers can
be rather demanding, but a semiconductor manufacturer loosing millions of
dollars every hour an instrument is down can be a real pain. Didn't sound
like too much fun to me.

Allen R. Sampson
Advanced Research Systems
317 North 4th. Street
St. Charles, Illinois 60174

phone (630) 513-7093 fax (630) 513-7092 http://www.sem.com


On Wednesday, January 12, 2005 9:39 AM, James M. Ehrman
[SMTP:jehrman-at-mta.ca] wrote:
}
}
}
------------------------------------------------------------------------
------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
-------
}
} Hi all,
}
} I'm looking at a paper that did some work with and Etec Autoscan. I'm not
} very familiar with the instrument, and no model number is given. Can
anybody
} fill me a bit on the history of this SEM? What is it's vintage, W, Lab6
} or FE, etc.?
} Does the company exist under another name? All the images and info I can
} find
} on the web look to be a 70s to 80s type instrument, but...
}
} Gee, a "History of Microscope Manufacturers: Intrigue, Bankruptcy and
} Hostile Takeovers"
} web site would be handy...
}
} As usual, thanks in advance!
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/dmf
}


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 13 17:20:59 2005



From: Peter McSwiggen :      PMcS-at-McSwiggenAssoc.com
Date: Thu, 13 Jan 2005 17:20:35 -0600
Subject: [Microscopy] Job posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all.

This position has come to my attention, and I thought it might be of
interest to the broader SEM community.

Regards,

Peter

Peter McSwiggen
McSwiggen & Associates, P.A.
2855 Anthony Lane South, Suite B1
St. Anthony, MN 55418
phone: 612.781.2282
fax: 612.781.7540
e-mail: PMcS-at-McSwiggenAssoc.com



_________________________
Guidant is seeking a SEM/EDS technician to operate a Scanning Electron
Microscope (SEM). This is a full time, 1st shift position in Guidant's
Corporate laboratory. Minimum requirements include: A two year
technical degree and 2 - 4 years of experience in Scanning Electron
Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS). Ability to
work independently and in a team oriented environment. Good writing
skills with an ability to independently write reports using standard
and custom software. Preferred qualifications include experience with
surface and failure analysis of electronic components in a FDA
regulated environment. Knowledge of material properties and
characteristics is a plus.

For more information or to apply contact:

Bruce Peacock
Sr. Scientist, Corporate Lab
Guidant Corporation
4100 Hamline Ave N.
St. Paul, MN 55112
Telephone: 651.582.2075
bruce.peacock-at-guidant.com



From MicroscopyL-request-at-ns.microscopy.com Fri Jan 14 03:55:37 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 06 Jan 2005 15:10:08 -0500
Subject: [Microscopy] JB-4 resin stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hi all,
I have a user who wants to stain lignin in JB-4 resin-embedded corn
stalks. He tried Phloroglucinol which works fine in paraffin embedded
samples since the paraffin is removed. However, this stain requires an HCl
treatment to reveal the desired color. The acid treatment interacts
adversely with the JB-4 resin.

The resin embedding gives much better resolution than the paraffin so it is
not an option to go back to the paraffin-embedded tissue.

Does anyone have an alternative stain that will work with this resin?

Thanks in advance,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy





From MicroscopyL-request-at-ns.microscopy.com Fri Jan 14 04:05:19 2005



From: Gareth Morgan :      Gareth.Morgan-at-labmed.ki.se
Date: Fri, 14 Jan 2005 15:26:36 +0100
Subject: [Microscopy] Re: Re: LKB Ultramicrotome III

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ephram

I thought that it was about mid 60's and Norma Reid's text on
ultramicrotomy cofirms that it was 1965. It was an improvement on
the 'Ultratome I' because it had finer manual cutting range and
extended cutting stroke as well as upgraded optics and a few other
things. There was never an 'Ultratome II' because this was the
designation for the upgrade kit for the I which turned it into
something like a III. We still occasionally use a I with a part II kit
modification.

Ref
Ultramicrotomy, Norma Reid (1975) (Part II of Vol 3 Practical Methods
in Electron Microscopy - Ed Audrey M. Glauert).

Hope this answers your question without giving you too much
information.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
} From: Ephram Shizgal {shizgal-at-lv-em.com}

Hi

Interesting about the Ultratome III coming out in '65. We bought one around
1978 I think and then after I had changed jobs, a IV in about 1984. So the
III was around for a long time.

Gareth



At 11:05 2005-01-14, Malcolm Haswell wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


With best wishes,

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.




From MicroscopyL-request-at-ns.microscopy.com Fri Jan 14 08:47:53 2005



From: lbalakrishnan-at-medicine.nodak.edu (by way of MicroscopyListserver)
Date: Fri, 14 Jan 2005 08:47:43 -0600
Subject: [Microscopy] [Filtered] AskAMicroscopist: visualize SV40 chromatin using TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (lbalakrishnan-at-medicine.nodak.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, January 13, 2005 at 11:03:29
---------------------------------------------------------------------------

Email: lbalakrishnan-at-medicine.nodak.edu
Name: Lata Balakrishnan

Organization: University of North Dakota

Education: Graduate College

Location: Grand Forks, North Dakota, USA

Question: Hi,
I have been trying to visualize SV40 chromatin using TEM. Our department does not have a glow discharge apparatus. So I am unable to do a glow discharge before applying my samples to the carbon coated formavar grid. Is there any other method that alternates the glow discharge so that the chromatin will adhere to the grid. At this point I am unable to pick up any chromatin just by rotary shadowing and tungsten coating. Also during tungsten coating sometimes the grid fries up and is compeletely broken. Any suggestions?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jan 14 08:48:52 2005



From: fahayes-at-comcast.net (by way of MicroscopyListserver)
Date: Fri, 14 Jan 2005 08:48:45 -0600
Subject: [Microscopy] viaWWW: used cryo ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (fahayes-at-comcast.net) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, January 13, 2005 at 12:50:22
---------------------------------------------------------------------------

Email: fahayes-at-comcast.net
Name: Fred Hayes

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I am interested in buying a used cryo ultramicrotome and glass knife maker that work. Please respond off line to fahayes-at-comcast.net

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jan 14 08:49:28 2005



From: derek.dunfield-at-gmail.com (by way of MicroscopyListserver)
Date: Fri, 14 Jan 2005 08:49:14 -0600
Subject: [Microscopy] viaWWW: Long Distance DIC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (derek.dunfield-at-gmail.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, January 13, 2005 at 19:28:08
---------------------------------------------------------------------------

Email: derek.dunfield-at-gmail.com
Name: Derek Dunfield

Organization: Brain Research Centre - UBC

Title-Subject: [Microscopy] [Filtered] MListserver: Long Distance DIC?

Question: Hello all,
Currently my lab is looking for a long distance (around 13mm or more depending on the shape of the objective - the longer the better) DIC or phase lens with high magnification (50X - 100X, again bigger is better). The idea is to use this lens with electrophysiology tools -hence the need for the long working distance. Do they exist? Any help would be most appreciated!

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jan 14 15:38:20 2005



From: David Hall :      hall-at-aecom.yu.edu
Date: Fri, 14 Jan 2005 16:37:38 -0500
Subject: [Microscopy] archive quality DVDs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are buying DVDs from a "namebrand" manufacturer for storing copies
of our digital data. I know that this discussion has gone around
before, but I am still curious.

I can buy "4X" speed DVD-Rs for about $1 each. The same namebrand
offers "8X" speed DVD-Rs for half the price, which presumably would
allow us to write or read the data twice as fast. And of course
off-brand DVDs are sold for much less.

I want to write data safely and reliably, so I'm paying for the
namebrand. Is it logical to also buy the slower access time DVD-Rs
at the higher price? I have heard horror stories about losing data
on cheaper media, or on media with the wrong style of writing
implement used to mark the contents.

Is 8X a safe bet, or should I stay with slower media?

Have a good weekend.
--
David H. Hall, Ph.D.
Center for C. elegans Anatomy
Department of Neuroscience
Albert Einstein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461

www.wormatlas.org
www.aecom.yu.edu/wormem

phone 718 430-2195
fax 718 430-2514


From MicroscopyL-request-at-ns.microscopy.com Fri Jan 14 16:44:29 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Sat, 15 Jan 2005 09:22:19 -0330
Subject: [Microscopy] RE: archive quality DVDs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

The act of having an image in focus in the TEM is when the objective lens
places the image on the exact plane being viewed by the diffraction lens.
Once these two lenses have been brought to this value the image will stay in
focus no matter how many lenses we place between this point, known as the
first image
plane, and any viewing device. Possible solutions are outlined below

1) I suggest that you set focus on your viewing screen (which matches the
two lenses as described above) and then adjust the other devices to bring
them into focus. I am not sure how difficult this may be but the problem is
not an instrument (microscope) focus problem.

2) The viewing device with the shorter specimen to device distance provides
you
with a lower magnification image than the device further away from the
specimen. As this is the case the lower device would be more critical of
the focus setting and maybe it is this that goes some way to that device
appearing out of focus. If you focus the image on the screen at say
200,000X with a 10X binocular and then display it upon the TV camera at
100,000X does the more critical image focus solve the problem? This assumes
that there is not a diffraction lens change between these two
magnifications. Such a change would change the desired objective lens
setting.

Good luck

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
www.emcourses.com tel +44 1280 816512 fax +44 1280 814007


----- Original Message -----
} From: "K.N. Bozhilov" {bozhilov-at-ucr.edu}
To: {microscopy-at-microscopy.com}
Sent: Wednesday, January 12, 2005 12:42 AM

David Hall writes ...

} I can buy "4X" speed DVD-Rs for about $1 each. The same namebrand
} offers "8X" speed DVD-Rs for half the price, which presumably would
} allow us to write or read the data twice as fast. And of course
} off-brand DVDs are sold for much less.
}
} I want to write data safely and reliably, so I'm paying for the
} namebrand. Is it logical to also buy the slower access time DVD-Rs
} at the higher price? I have heard horror stories about losing data
} on cheaper media, or on media with the wrong style of writing
} implement used to mark the contents.

You are correct regarding "cheap" media, and you are somewhat safe buying
name brands, but not entirely, because you can buy the same name brand one
month and realize it is made in Japan, and buy the same again the next month
and realize after it is made by a different process in Taiwan.

Many will also imply the safest write speed is the slowest, ... e.g., to
purchase any reputable media and always write at 2.4x ... with patience, and
enable verification after the write. It's your data!

Here is a reputable and informative website:

http://www.videohelp.com

hth & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Sun Jan 16 18:22:28 2005



From: Sally Stowe :      Sally.Stowe-at-anu.edu.au
Date: Mon, 17 Jan 2005 11:24:55 +1100
Subject: [Microscopy] Position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
We have a position available shortly in our unit - see details below. The
selection criteria etc should be available at the given web address next
week, or email Alanah.McCann-at-anu.edu.au
Briefly, we are a campus-wide unit doing approximately 60% materials work
and 40% biological, 3 TEMs, 4 SEMs, a FIB/SEM, 3 EDXAs and light
microscopes, cryo-gear. The work will involve user support, instrument
maintenance and development.

Regards
Sally Stowe

Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
The Australian National University
www.anu.edu.au/EMU/index.htm
sally.stowe-at-anu.edu.au
GPO Box 475,Canberra ACT 2601 Australia
ANU CRICOS#00120C


//////////////////////////////////////////////////////////

ANU Electron Microscopy Unit
Technical Officer

ANU OFFICER GRADE 4/5

Salary Package: $39,341 - $47,861 pa plus 17% Super

We are seeking a highly motivated person to join the staff team in a
cross-disciplinary microscopy facility. Knowledge and expertise in fields
relevant to scientific instrumentation such as electronic or mechanical
engineering, computing and image analysis would be an advantage, as would
direct experience with electron microscopy.
The web address for the unit is www.anu.edu.au/EMU/index.htm
Selection Criteria:
http://info.anu.edu.au/hr/Jobs/General_Positions/_PDF/QESS .pdf or
email: Alanah.McCann-at-anu.edu.au, phone 6125 4138.

Further Enquiries: Sally.stowe-at-anu.edu.au

Closing date: 18 February 2005

___________________________________________________________________________


Information to applicants available from
http://info.anu.edu.au/hr/Jobs/How_To_Apply/_Academic_Info.pdf.

Please complete the compulsory cover sheet and attach it to your application
- http://info.anu.edu.au/policies/Forms/Human_Resources/HR86.asp




From MicroscopyL-request-at-ns.microscopy.com Sun Jan 16 21:42:55 2005



From: ronpeters-at-integraonline.com (by way of Ask-A-Microscopist)
Date: Sun, 16 Jan 2005 21:45:42 -0600
Subject: [Microscopy] [Filtered] AskAMicroscopist: Oil vs Homogenous Immersion Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ronpeters-at-integraonline.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Sunday, January 16, 2005 at 16:01:22
---------------------------------------------------------------------------

Email: ronpeters-at-integraonline.com
Name: Ron Peters

Education: Graduate College

Location: Prior Lake, MN, USA

Question: I am confused about the exact difference between objectives
marked "Oil" immersion and those marked Homogenous Immersion ("HI").
What exactly are the differences in applying these two different
objectives? Does an HI objective use a cover slip? Does an HI
objective use the same immersion oil as an "Oil" objective?

Thanks for your help.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sun Jan 16 21:44:47 2005



From: ronpeters-at-integraonline.com (by way of Ask-A-Microscopist)
Date: Sun, 16 Jan 2005 21:47:13 -0600
Subject: [Microscopy] [Filtered] AskAMicroscopist: Oil vs Homogenous Immersion Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ronpeters-at-integraonline.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Sunday, January 16, 2005 at 16:01:22
---------------------------------------------------------------------------

Email: ronpeters-at-integraonline.com
Name: Ron Peters

Education: Graduate College

Location: Prior Lake, MN, USA

Question: I am confused about the exact difference between objectives
marked "Oil" immersion and those marked Homogenous Immersion ("HI").
What exactly are the differences in applying these two different
objectives? Does an HI objective use a cover slip? Does an HI
objective use the same immersion oil as an "Oil" objective?

Thanks for your help.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sun Jan 16 22:18:51 2005



From: Joe Kulik :      juk12-at-psu.edu
Date: Tue, 18 Jan 2005 16:46:38 -0500
Subject: [Microscopy] TEM -- Job Opening for Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I found from experience that the side port (35mm port) on most TEMs is not
parfocal with the plate camera film or the bottom port. Some TEMs are close
enough, others are not. There are a few exceptions, most notably the
JEOL-100C (and 200C), which have side ports that are dead-on parfocal with
the focusing screen, plate camera film, and bottom port.

The whole issue arises in the following cases:

1) A side mounted CCD camera (instead of binoculars and focusing screen) is
used to focus for a film plate camera (a bad idea unless the camera is low
view angle and side port is parfocal with the plate film).

2) A TEM focusing screen with binoculars is used to focus for a side mounted
slow scan camera that has no live focus capability.

3) A bottom mounted TV camera is used as a focusing aid for a side mounted
slow scan CCD. Such configuration is not common.

Of course, cases (2) and (3) are no problem for a 1K x 1K or fewer pixel
count side mounted CCD camera with a wide viewing angle, but a side mounted
camera of more than 2 megapixels had better have a live focus capability.
Otherwise, some focus correction will probably be required compared to the
focusing screen or bottom mounted TV camera. The higher the pixel density
is, the more noticeable a focus difference becomes.

I know that the entire space below the final projector lens is considered to
be parfocal (including several floors below the TEM :-). In practice,
however, this is not always the case. I should confess that I didn't study
this condition beyond accumulating TEM brand/model- specific statistics.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane
Duluth, GA 30096
Tel. (770)232-7785
Fax (770)232-1791
Mobile (678)467-0012
www.sia-cam.com
----- Original Message -----
} From: "K.N. Bozhilov" {bozhilov-at-ucr.edu}
To: {microscopy-at-microscopy.com}
Sent: Tuesday, January 11, 2005 7:42 PM


Electron Microscopy Technician

The Materials Characterization Laboratory, part of Penn State's
Materials Research Institute, has an immediate opening for an
experienced electron microscopy technician. Responsibilities will
include overseeing the maintenance on three transmission electron
microscopes (TEM), one focused ion beam (FIB) and a variety of support
equipment. Applicants should have hands-on experience operating and
repairing electron columns, vacuum systems and electronics. Other
responsibilities will include maintaining sample preparation equipment
such as diamond saws, ion thinners, polishing equipment as well as
training users in the operation of that equipment.

MINIMUM QUALIFICATIONS:
Associate's degree in a technical or administrative program, or
equivalent knowledge, plus 1 year related work experience.

Send resume and cover letter (by e-mail, USPS, or courier) to:
Joe Kulik
The Pennsylvania State University
194 Materials Research Institute Building
University Park, PA 16802-7003

e-mail: juk12-at-psu.edu



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 19 04:36:10 2005



From: Beaurega :      beaurega-at-westol.com
Date: Tue, 18 Jan 2005 14:11:16 -0500
Subject: [Microscopy] Re: RE: RE: RE: SEM: gun saturation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Michael,

Did anyone ever answer your question?
You asked, "Why don't we see the monotonic rise as described by
"self-biasing", but instead hills and valleys?"

I would be interested in reading the answers you received to this question.

Since we can't post attachments, I looked for some slides on the internet
for me to discuss how this circuit works (between looking for a position in
microscopy). I was pleasantly surprised to find a PDF file at my old alma
mater, Ohio State University, in the geology department no less. See pages
17 and 18 below.
http://www.geology.ohio-state.edu/~bhattiprolu/GS675/notes/lecture1.pdf

This PDF file shows a general characteristic curve shape on page 18.

My background in electronics, as related to microscopy and other areas, is
below.

Paul Beauregard
Chemist (Ohio State) & Microscopist.
Electronics, University of Akron, Summa Cum Laude.
FCC licensed commercial radiotelephone license holder.
FCC extra class license holder, station call sign is KC8O /3.
• — • — • • • • — • —





From MicroscopyL-request-at-ns.microscopy.com Wed Jan 19 10:01:44 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Wed, 19 Jan 2005 10:04:52 -0600
Subject: [Microscopy] MM2005 Submission Deadline - Feb 15th 2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Just a friendly reminder that the deadline for submission of Manuscripts
for Microscopy & Microanalysis 2005 if Feb 15th, just under a month away.


On behalf of the MM2005 Program Committee

Nestor
Your Friendly Neighborhood SysOp.



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 19 10:48:58 2005



From: Franklin Bailey :      jfb-at-uidaho.edu
Date: Wed, 19 Jan 2005 08:57:44 -0800
Subject: [Microscopy] Re: RE: RE: RE: SEM: gun saturation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't know how much stock I would place in the Ohio State set of notes
since they name wavelength as the chief factor in SEM resolution instead of
probe size.

Franklin Bailey
University of Idaho
Electron Microscopy Center


-----Original Message-----
} From: Beaurega [mailto:beaurega-at-westol.com]
Sent: Tuesday, January 18, 2005 11:11 AM
To: michael shaffer; MSA listserver

Dear Michael,

Did anyone ever answer your question?
You asked, "Why don't we see the monotonic rise as described by
"self-biasing", but instead hills and valleys?"

I would be interested in reading the answers you received to this question.

Since we can't post attachments, I looked for some slides on the internet
for me to discuss how this circuit works (between looking for a position in
microscopy). I was pleasantly surprised to find a PDF file at my old alma
mater, Ohio State University, in the geology department no less. See pages
17 and 18 below.
http://www.geology.ohio-state.edu/~bhattiprolu/GS675/notes/lecture1.pdf

This PDF file shows a general characteristic curve shape on page 18.

My background in electronics, as related to microscopy and other areas, is
below.

Paul Beauregard
Chemist (Ohio State) & Microscopist.
Electronics, University of Akron, Summa Cum Laude.
FCC licensed commercial radiotelephone license holder.
FCC extra class license holder, station call sign is KC8O /3.
• — • — • • • • — • —






From MicroscopyL-request-at-ns.microscopy.com Wed Jan 19 11:35:44 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Wed, 19 Jan 2005 14:07:04 -0330
Subject: [Microscopy] RE: Re: RE: RE: RE: SEM: gun saturation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Franklin Bailey writes ...

} I don't know how much stock I would place in the Ohio State set of notes
} since they name wavelength as the chief factor in SEM resolution
} instead of probe size.

One of the primary factors which minimize the spot size is "diffraction
aberration", a function of wavelength ... is it not(?)

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
{www.micro-investigations.com}

} -----Original Message-----
} From: Beaurega [mailto:beaurega-at-westol.com]
} Sent: Tuesday, January 18, 2005 11:11 AM
} To: michael shaffer; MSA listserver
} Subject: [Microscopy] Re: RE: RE: RE: SEM: gun saturation
}
}
}
}
} ------------------------------------------------------------------
} ----------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.msa.microscopy.com/MicroscopyListserver
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------
} ----------
} ---
}
} Dear Michael,
}
} Did anyone ever answer your question?
} You asked, "Why don't we see the monotonic rise as described by
} "self-biasing", but instead hills and valleys?"
}
} I would be interested in reading the answers you received to this
} question.
}
} Since we can't post attachments, I looked for some slides on the internet
} for me to discuss how this circuit works (between looking for a
} position in
} microscopy). I was pleasantly surprised to find a PDF file at my old alma
} mater, Ohio State University, in the geology department no less.
} See pages
} 17 and 18 below.
} http://www.geology.ohio-state.edu/~bhattiprolu/GS675/notes/lecture1.pdf
}
} This PDF file shows a general characteristic curve shape on page 18.
}
} My background in electronics, as related to microscopy and other areas, is
} below.
}
} Paul Beauregard
} Chemist (Ohio State) & Microscopist.
} Electronics, University of Akron, Summa Cum Laude.
} FCC licensed commercial radiotelephone license holder.
} FCC extra class license holder, station call sign is KC8O /3.
} • — • — • • • • — • —
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 19 19:20:43 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 19 Jan 2005 17:23:05 -0800
Subject: [Microscopy] Holey film references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleague
I am working on article regarding holey film preparation technique I
succesfully use for two decades. I want to present historical data on this
issue and sort of summary on different techniques used in EM.
Unfortunately, most of the work on holey film preparation done in 50-60es
and is not indexed in modern databases and my personal archive was lost
when I moved to US. So, I could not restore some important references. I
would greatly appreciate your help in pointing on old references/articles
on holey film preparation I could cited/used in my work. I would be happy
to share the information with EM community. Thanks for your help in
advance, Sergey

P.S. Any suggestions where I could publish such work (with detailed
instruction how to make holey films) would be greatly appreciated also.
Have a great day.

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Thu Jan 20 17:12:49 2005



From: John Shields :      jpshield-at-uga.edu
Date: Thu, 20 Jan 2005 18:15:19 -0500
Subject: [Microscopy] tired of re: re:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm not even opening those...
John P. Shields
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602

706-542-4080


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 20 21:31:05 2005



From: kssim-at-mmu.edu.my (by way of MicroscopyListserver)
Date: Thu, 20 Jan 2005 21:33:54 -0600
Subject: [Microscopy] viaWWW: petrographic microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kssim-at-mmu.edu.my) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, January 20, 2005 at 20:31:36
---------------------------------------------------------------------------

Email: kssim-at-mmu.edu.my
Name: kssim

Organization: mmu

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear All,

I have a feew question regarding petrographic microscope. does anyone know the process of preparing petrographic sample for imaging ? Please kindly share with me your valubale knowledge.

Thanks
Dr. Ks Sim


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 20 21:54:07 2005



From: vleppert-at-ucmerced.edu
Date: Fri, 21 Jan 2005 12:56:47 +0900
Subject: [Microscopy] Facility Manager Position - UC Merced

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We are hiring a facility manager for the Imaging and Microscopy Facility at UC Merced (primarily for electron microscopy). UC Merced is the newest campus in the University of California system, located approximately 2 hours SE of the San Francisco Bay Area, and will open to 1,000 students in the Fall of 2005. Eventual enrollment is expected to be 25,000 students. Please see the job posting below for more details.

http://jobs.ucmerced.edu/view_staff_position.faces?positionId=52

--------------------------------------------------------
Valerie J. Leppert, Assistant Professor
University of California, Merced
School of Engineering

Mailing Address:
P.O. Box 2039
Merced, CA 95344

Physical Address (for couriers/parcels):
4225 N. Hospital Road, Bldg 1200
Atwater, CA 95301

tel: (209) 724-4365
fax: (209) 724-2912
em: vleppert-at-ucmerced.edu



From MicroscopyL-request-at-ns.microscopy.com Fri Jan 21 08:34:15 2005



From: Phaedra McGuinness :      scanning-at-fams.org
Date: Fri, 21 Jan 2005 09:36:34 -0500
Subject: [Microscopy] SCANNING 2005 Call for Papers Invitation and Meeting Information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Friends:

***Mark your calendar now and plan to attend SCANNING 2005 in beautiful
Monterey, California, Tuesday April 5 through Thursday April 7.***
The following information is available on the SCANNING website at
www.scanning.org.

• 2nd Call for Papers and Abstract Preparation Details
• The Preliminary Program for SCANNING 2005 including speakers and
abstract titles
• Complete Short Course Descriptions
• Official Registration Form
• 3 Days of Forensics including An Evening with Skip Palenik
• Brand New Sessions!
• Schedule of 10 MUST-SEE Vendor Tutorials at SCANNING 2005

Schedule of TUTORIALS
Vendor Date Time
CamScan USA, Inc. Tuesday, April 5 10:00am-10:30am
Oxford Instruments Tuesday, April 5 12:30pm-1:30pm
Piezosystem Jena, Inc. Tuesday, April 5 3:00pm-3:30pm
BioForce Nanosciences Inc. Wednesday, April 6 10:00am-10:30am
Carl Zeiss SMT, Inc. Wednesday, April 6 12:30pm-1:30pm
EDAX/TSL Wednesday, April 6 3:00pm-3:30pm
IXRF Wednesday, April 6 5:00pm–6:00pm
PI Thursday, April 7 10:00am-10:30am
Soft Imaging System Thursday, April 7 12:30pm-1:30pm
xk, Inc. Thursday, April 7 3:00pm-5:00.pm (in forensics meeting
room)

• Hotel and Airline Accommodations Available (Official carrier offering
domestic and international discounts is United Airlines)
• Book and Software Exhibit Application
• Monterey Bay Aquarium Outing and Other Social Events
• Listing of SCANNING 2005 Sponsors and Contact Information

We thank you all for your past support of the SCANNING meetings and
look forward to greeting you in Monterey. Please don't hesitate to
contact us direct with queries or to request additional information.


Best regards,


Phaedra McGuinness
Managing Editor
SCANNING, The Journal of Scanning Microscopies
P.O. Box 485
Mahwah, NJ 07430
Tel: (201) 818-1010 * Fax: (201) 818-0086 *email: scanning-at-fams.org *
Web: www.scanning.org




From MicroscopyL-request-at-ns.microscopy.com Fri Jan 21 09:34:39 2005



From: Michal Jarnik :      M_Jarnik-at-fccc.edu
Date: Fri, 21 Jan 2005 10:37:06 -0500
Subject: [Microscopy] Neurons on membranes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We need to Epon embed and section neurons growing attached to a
membrane. In the past (relatively long ago), we used to use millipore
membrane inserts treated with poly-Lys. The neurons stuck very well to
the membrane and more over, the membrane "curled" nicely during the
processing giving us effectively larger surface cut on each section and
so better chance to find what we needed. Currently, our neurons refuse
to stay on the membrane and in addition, the membrane stays flat. I
remember a discussion about the topic some time ago, but have problems
to find it in the archives. Does anybody have a good idea?

Thanks,

Michael Jarnik,
FCCC, Philadelphia



From MicroscopyL-request-at-ns.microscopy.com Fri Jan 21 11:31:07 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Sat, 22 Jan 2005 11:32:25 -0600
Subject: [Microscopy] Re: viaWWW: petrographic microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

The simple answer is that, typically, a slab of rock is cut off with a diamond saw, glued to a petrographic slide, then ground and polished to an appropriate thickness (ex: 30microns). If it is a mineral that contains quartz, the 30micron thickness is evaluated by its color between crossed polars (146nm retardation). For more complete information and tips regarding specific minerals, I'd recommend your contacting
Buehler, Struers, Logitech, or Mark V Labs. They all have the necessary equipment, supplies and expertise.

One other note: petrographic slides are not the same size as normal 1"x3" microscope slides.

Hope this was helpful,

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.



At 09:33 PM 1/20/2005, kssim-at-mmu.edu.my wrote:


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From MicroscopyL-request-at-ns.microscopy.com Fri Jan 21 20:16:25 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Fri, 21 Jan 2005 18:18:23 -0800
Subject: [Microscopy] Re: Holey film references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Sergey,
Do you have access to Desmond Kay's Techniques for Electron Microscopy,
2nd edition, 1965?
If so, Chapter 3 was written by D. E. Bradley , pg 58-74, on The
Preparation of Specimen Support Films and has early references for it.
It covers several of the early techniques.

Kay, Desmomd (Ed), 1965, Techniques for Electron Microscopy, F. A.
Davis, Co., Philadelphia, PA

If you do not have access to it, I can scan in the references for you
and send them to you off line.

If it is a new technique for making holey grids, then perhaps the
Microscopy and Microanalysis Journal which is the official journal of
the Microscopy Society of America (see microscopy.com and follow
information to the journal). Also, you might consider Journal of
Microscopy Research and Techniques. If it is just a short article, you
might consider Microscopy Today (information also available at
microscopy.com).

Best of Luck,
Judy Murphy
Stockton, CA


Sergey Ryazantsev wrote:

}
}
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}
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} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Dear Colleague
} I am working on article regarding holey film preparation technique I
} succesfully use for two decades. I want to present historical data on
} this issue and sort of summary on different techniques used in EM.
} Unfortunately, most of the work on holey film preparation done in
} 50-60es and is not indexed in modern databases and my personal
} archive was lost when I moved to US. So, I could not restore some
} important references. I would greatly appreciate your help in pointing
} on old references/articles on holey film preparation I could
} cited/used in my work. I would be happy to share the information with
} EM community. Thanks for your help in advance, Sergey
}
} P.S. Any suggestions where I could publish such work (with detailed
} instruction how to make holey films) would be greatly appreciated
} also. Have a great day.
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} 10833 Le Conte Ave, Room 33-080
} Los Angeles, CA 90095
}
} Phone: (310) 825-1144 (office)
} (310) 206-1029 (Lab)
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Sat Jan 22 03:33:22 2005



From: Elisabeth Albert :      elisabethalbert32-at-plugin.com.br
Date: Sat, 22 Jan 2005 10:18:01 +0000
Subject: [Microscopy] Tadalafil Soft Tabs - Great results!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

We have a new product that we offer to you, C_I_A_L_I_S soft tabs,

Cialis Soft Tabs is the new impotence treatment drug that everyone is talking
about.Soft Tabs acts up to 36 hours, compare this to only two or three hours
of Viagra action! The active ingredient is Tadalafil, same as in brand Cialis.

Simply disolve half a pill under your tongue, 10 min before sex, for the best
erections you've ever had!

Soft Tabs also have less sidebacks (you can drive or mix alcohol drinks with them).

You can get it at: http://onlinegenericshop.com/soft/





No thanks: http://onlinegenericshop.com/rr.php



From MicroscopyL-request-at-ns.microscopy.com Sat Jan 22 11:48:15 2005



From: germaine_g_boucher-at-groton.pfizer.com (by way of MicroscopyListserver)
Date: Sat, 22 Jan 2005 11:50:44 -0600
Subject: [Microscopy] viaWWW: reprocessing specimens from paraffin blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (germaine_g_boucher-at-groton.pfizer.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, January 21, 2005 at 13:07:37
---------------------------------------------------------------------------

Email: germaine_g_boucher-at-groton.pfizer.com
Name: Germaine Boucher

Organization: Pfizer

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Does anyone out there have a protocol for reprocessing specimens from paraffin blocks for TEM?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Jan 22 17:51:37 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Sat, 22 Jan 2005 15:53:16 -0800
Subject: [Microscopy] Re: viaWWW: reprocessing specimens from paraffin blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Reprocessing paraffin blocks

Hi Germaine,
Normally we dealt with sections of paraffin blocks as we wanted a
specific area.

For fiber and mineral content:
1. We used a conventional 6 micron section from a paraffin block.
2. We mounted it on a glass slide treated it with xylene and alcohol to
remove the wax.

For fiber and mineral content:
If we were doing fiber analysis in the sample, we allowed it to dry,
ashed it in a muffle furnace at 450 degrees C until the tissue was
completely ashed.
3. Added polyvinyl alcohol (PVA) solution to the specimen area, and
allowed it to dry.
4. Peeled off the hardened film.
5. Placed hardened film upside down on a slide.
6. Evaporated carbon on upper face of hardened film.
7. Floated carbonized specimen onto the surface of hot water. This
dissolves the water-soluble plastic and leaves the carbon film and the
contained ashed tissue floating on the surface.
8. Gently, broke up the film into 3 mm squared pieces.
9. Picked up pieces on EM grid and examined

This was used way back in the seventies by F.C.Pooley and details
published by
Langer, A.M. et al, 1972. Chemical Characterization of Uncoated
Asbesots Fibers from the lungs of Asbestos Workers by Electron
Microproble Analysis. J. Histochem. Cytochem 20:735.

To re-embed for TEM:
After 1 and 2 above
3. Once in alcohol, changed 2 or 3 times to be sure it was dehydrated
in 100% EtOH.
4. For Epon Resin Mixture: further dehydrated in propylene oxide, 2
times 10 min each on the slide.
5. Added graded series of PO:Epon replacement mix(2:1; 1:1; 1:2) for 30
min each
6. Changed to pure epon mix with 2 changes for 30 min each, on the slide
7. Took off excess resin from slide with sample, and put a full
capsule (full of epon mix) over the slide.
8. Put in oven (60C) and hardened it.
9. After hardening, can pop it off by putting in liquid nitrogen
quickly and one gets the section at the tip of the capsule.
10. We developed a slide holder (sold yrs ago by Polysciences) to hold
the slide while it was easily popped off, but anything will do just so
it is held so the pressure point is at the capsule/slide interface.

NOTES:
A. If LRWhite is used, the propylene oxide was not used and we
prevented air from getting to the mixture when being polymerized. Have
occasionally used propylene oxide by mistake over the yrs and that seems
to work as well with LR White but generally do not use it with LR
White. With LR White, we hardened at 52C if we were not in a hurry or 1
hr at 90C, if we had to do the same day.
B. Most EM Supply companies sell Epon substitute (Ted Pella, EMS,
Fullam, SPI, etc.) We used Glauerts medium mix for hardness. All Epon
mixes had the catalyst in them when used.
C. In later yrs , we used the microwave for the later stages after the
paraffin was gone. Paraffin is transparent to microwaves so doesn't
melt in the microwave, just like a dry ice cube doesn't melt because it
also is transparent to the microwaves! (As an aside, that is a great
bar trick! - before you bet, just be sure the ice cube is dry of
moisture which acts as a catalyst for melting). If you use a microwave,
use one with a cooled specimen stage. The microwave speeds up the
process tremendously and it is done in no time and one can section and
do the analysis the same day easily with time to spare.

I have not tried the entire paraffin block as usually I had specific
areas I was looking for so had to section the paraffin block to find out
where I wanted to look. It would seem that the same process would work,
however I would think the times would have to be increased somewhat. Of
course, as you know, section or block, the ultrastructure is only as
good as the fixation and preparation was initially when the sample was
fresh!!!

Hope that is helpful,
Judy

Judy Murphy, PhD
Microscopy & Imaging Consultant
Stockton, CA
murphyjudy-at-comcast.net



by way of MicroscopyListserver wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Sat Jan 22 19:30:06 2005



From: pilttdownman-at-wmconnect.com (by way of Ask-A-Microscopist)
Date: Sat, 22 Jan 2005 19:32:37 -0600
Subject: [Microscopy] AskAMicroscopist: cardioid condensor vs star

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pilttdownman-at-wmconnect.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, January 22, 2005 at 18:06:32
---------------------------------------------------------------------------

Email: pilttdownman-at-wmconnect.com
Name: Ron Joyner

Organization: Mississippi Gulfcoast community college

Education: Undergraduate College

Location: Gautier, Mississippi

Question: what advantage does a cardioid condensor have over a star diaphragm?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Jan 22 20:41:38 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Sat, 22 Jan 2005 18:43:16 -0800
Subject: [Microscopy] Re: Re: viaWWW: reprocessing specimens from paraffin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
This is a post script for the procedure below for deparaffinization of
materials.

If one is doing morphology the below procedure which is listed below
will work however for quantitative numbers of fibers which must be
identified particularly by diffraction, plasma ashing should be used to
prevent the asbestos from changing its crystallinity structure which of
course prevents a correct diffraction pattern.

It is good to have good friends that help point out missing information
especially important missing information. Thanks Chuck,

Judy Murphy

Judy Murphy wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Reprocessing paraffin blocks
}
} Hi Germaine,
} Normally we dealt with sections of paraffin blocks as we wanted a
} specific area.
}
} For fiber and mineral content:
} 1. We used a conventional 6 micron section from a paraffin block.
} 2. We mounted it on a glass slide treated it with xylene and alcohol
} to remove the wax.
}
} For fiber and mineral content:
} If we were doing fiber analysis in the sample, we allowed it to dry,
} ashed it in a muffle furnace at 450 degrees C until the tissue was
} completely ashed.
} 3. Added polyvinyl alcohol (PVA) solution to the specimen area, and
} allowed it to dry.
} 4. Peeled off the hardened film.
} 5. Placed hardened film upside down on a slide.
} 6. Evaporated carbon on upper face of hardened film.
} 7. Floated carbonized specimen onto the surface of hot water. This
} dissolves the water-soluble plastic and leaves the carbon film and the
} contained ashed tissue floating on the surface.
} 8. Gently, broke up the film into 3 mm squared pieces.
} 9. Picked up pieces on EM grid and examined
}
} This was used way back in the seventies by F.C.Pooley and details
} published by
} Langer, A.M. et al, 1972. Chemical Characterization of Uncoated
} Asbesots Fibers from the lungs of Asbestos Workers by Electron
} Microproble Analysis. J. Histochem. Cytochem 20:735.
}
} To re-embed for TEM:
} After 1 and 2 above
} 3. Once in alcohol, changed 2 or 3 times to be sure it was dehydrated
} in 100% EtOH.
} 4. For Epon Resin Mixture: further dehydrated in propylene oxide, 2
} times 10 min each on the slide.
} 5. Added graded series of PO:Epon replacement mix(2:1; 1:1; 1:2) for
} 30 min each
} 6. Changed to pure epon mix with 2 changes for 30 min each, on the slide
} 7. Took off excess resin from slide with sample, and put a full
} capsule (full of epon mix) over the slide.
} 8. Put in oven (60C) and hardened it.
} 9. After hardening, can pop it off by putting in liquid nitrogen
} quickly and one gets the section at the tip of the capsule.
} 10. We developed a slide holder (sold yrs ago by Polysciences) to
} hold the slide while it was easily popped off, but anything will do
} just so it is held so the pressure point is at the capsule/slide
} interface.
}
} NOTES:
} A. If LRWhite is used, the propylene oxide was not used and we
} prevented air from getting to the mixture when being polymerized.
} Have occasionally used propylene oxide by mistake over the yrs and
} that seems to work as well with LR White but generally do not use it
} with LR White. With LR White, we hardened at 52C if we were not in a
} hurry or 1 hr at 90C, if we had to do the same day.
} B. Most EM Supply companies sell Epon substitute (Ted Pella, EMS,
} Fullam, SPI, etc.) We used Glauerts medium mix for hardness. All
} Epon mixes had the catalyst in them when used.
} C. In later yrs , we used the microwave for the later stages after
} the paraffin was gone. Paraffin is transparent to microwaves so
} doesn't melt in the microwave, just like a dry ice cube doesn't melt
} because it also is transparent to the microwaves! (As an aside, that
} is a great bar trick! - before you bet, just be sure the ice cube is
} dry of moisture which acts as a catalyst for melting). If you use a
} microwave, use one with a cooled specimen stage. The microwave speeds
} up the process tremendously and it is done in no time and one can
} section and do the analysis the same day easily with time to spare.
}
} I have not tried the entire paraffin block as usually I had specific
} areas I was looking for so had to section the paraffin block to find
} out where I wanted to look. It would seem that the same process would
} work, however I would think the times would have to be increased
} somewhat. Of course, as you know, section or block, the
} ultrastructure is only as good as the fixation and preparation was
} initially when the sample was fresh!!!
}
} Hope that is helpful,
} Judy
}
} Judy Murphy, PhD
} Microscopy & Imaging Consultant
} Stockton, CA
} murphyjudy-at-comcast.net
}
}
}
} by way of MicroscopyListserver wrote:
}
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } submitted by (germaine_g_boucher-at-groton.pfizer.com) from
} } http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
} } January 21, 2005 at 13:07:37
} } ---------------------------------------------------------------------------
} }
} }
} } Email: germaine_g_boucher-at-groton.pfizer.com Name: Germaine Boucher
} }
} } Organization: Pfizer
} }
} } Title-Subject: [Microscopy] [Filtered] MListserver:
} }
} } Question: Does anyone out there have a protocol for reprocessing
} } specimens from paraffin blocks for TEM?
} }
} } ---------------------------------------------------------------------------
} }
} }
} }
} }
} }
}
}


From MicroscopyL-request-at-ns.microscopy.com Sun Jan 23 08:45:24 2005



From: PETER HEIMANN :      peter.heimann-at-uni-bielefeld.de
Date: Mon, 24 Jan 2005 11:48:10 +0100
Subject: [Microscopy] formula/composition for Ilford PERCEPTOL B/W developer ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

Thanks for clarify my about the petrographic microscope. It is valuable
information.

Again thanks for helping me
Ks
----- Original Message -----
} From: "Barbara Foster" {bfoster-at-mme1.com}
To: "by way of MicroscopyListserver" {kssim-at-mmu.edu.my} ;
{microscopy-at-microscopy.com}
Sent: Sunday, January 23, 2005 1:32 AM

Colleagues,
can anybody provide the
formula/composition for Ilford PERCEPTOL B/W developer ?
Obviously the photochemical department of ILFORD (UK) has filed
{se?lp=ende&p=/Mn4k.&search=file} a {se?lp=ende&p=/Mn4k.&search=a}
petition {se?lp=ende&p=/Mn4k.&search=petition} in
{se?lp=ende&p=/Mn4k.&search=in} bankruptcy and I can't get / order the
above cited developer anymore here in Germany.
{se?lp=ende&p=/Mn4k.&search=bankruptcy}
I need this developer for developing ("tenderize") the B/W ortho-films I
use with my ZEISS EM109 TEM.
Thanks for any advice!
Peter Heimann



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 24 08:29:34 2005



From: ecd10-at-psu.edu (by way of MicroscopyListserver)
Date: Mon, 24 Jan 2005 08:35:20 -0600
Subject: [Microscopy] viaWWW: POST-DOCTORAL POSITION in AEM at PSU

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ecd10-at-psu.edu) from http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html on Monday, January 24, 2005 at 08:15:03
---------------------------------------------------------------------------

Email: ecd10-at-psu.edu
Name: Elizabeth Dickey

Organization: Penn State

Title-Subject: [Microscopy] [Filtered] MListserver: Post-doc Opening at Penn State

Question:
POST-DOCTORAL POSITION in Analytical Transmission Electron Microscopy
at The Pennsylvania State University



A postdoctoral position is available in the area of analytical transmission electron microscopy beginning March 1, 2005. The research project focuses on understanding structure and chemistry of amorphous metal oxides for capacitor and IR sensor applications. Through a variety of electron imaging, spectroscopy and diffraction (e.g. fluctuation EM) techniques we aim to quantify structure and chemistry of amorphous metal-oxide layers to help establish processing/structure/property relationships for this class of materials. Furthermore, we aim to understand the microstructural and microchemical evolution under applied bias. Most of the research will be conducted on a JEOL 2010F Field Emission TEM/STEM outfitted with an EDAX energy dispersive x-ray spectrometer (EDS), Gatan Enfina electron energy loss spectrometer (EELS), high-angle annular dark field STEM detector, and acquisition hardware and software for spectrum imaging. The ideal candidate for this position will have experience in HREM, STEM, EDS and EELS. The salary will be commensurate with qualifications and experience. Exceptionally qualified candidates may be considered at the Research Associate level.

Please forward questions or send applications to:

Professor Elizabeth Dickey
Department of Materials Science and Engineering
The Pennsylvania State University
223 Materials Research Building
University Park, PA 16802
USA

tel: (814) 865-9067
FAX: (814) 865-2326
email: ecd10-at-psu.edu



---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 24 09:09:33 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 24 Jan 2005 10:13:56 -0800
Subject: [Microscopy] Re: formula/composition for Ilford PERCEPTOL B/W

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There was a well-known British photographer who died suddenly last year
at a relatively young age, worked mostly (exclusively?) in B&W, can't
remember his name, Barry something or other? I have his book at home, I
think he used his own version of Perceptol, I will check at home
tonight. I did not find Perceptol in Steve Anchell's books.

Geoff

PETER HEIMANN wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Colleagues,
} can anybody provide the
} formula/composition for Ilford PERCEPTOL B/W developer ?
} Obviously the photochemical department of ILFORD (UK) has filed
} {se?lp=ende&p=/Mn4k.&search=file} a {se?lp=ende&p=/Mn4k.&search=a}
} petition {se?lp=ende&p=/Mn4k.&search=petition} in
} {se?lp=ende&p=/Mn4k.&search=in} bankruptcy and I can't get / order the
} above cited developer anymore here in Germany.
} {se?lp=ende&p=/Mn4k.&search=bankruptcy}
} I need this developer for developing ("tenderize") the B/W ortho-films
} I use with my ZEISS EM109 TEM.
} Thanks for any advice!
} Peter Heimann
}
}

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From MicroscopyL-request-at-ns.microscopy.com Mon Jan 24 09:44:11 2005



From: :      mcmullen1218-at-sbcglobal.net (by way of Ask-A-Microscopist)
Date: Mon, 24 Jan 2005 09:49:41 -0600
Subject: [Microscopy] AskAMicroscopist : scanning electron microscopy of Protein

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My name is Erin Wagner. I am currently a graduate student at the University of the Pacific in Stockton, Ca. Recently, I have been reviewing the literature for my graduate project and have found some difficulty in retrieving the information I am looking for. While researching on-line I ran across your website. I was wondering if you would be willing to help me find the information I have been looking for.

For my project I am studying a protein that may be involved in cross-linkage and we would like to confirm this using SEM. However, the protein is embeded in a fiber and the only way to expose the protein is to solubilize the fiber in guanine-HCL. The fiber can be transferred to a solution less harsh such as 50 mM Tris-HCL by dialysis but the fiber is not soluble in water. I was wondering if SEM is possible under such conditions and if so whether you might know a protocol or where I could find a protocol to conduct the experiment. All the papers I have found discuss growing tissue on a slide, since my sample is a protein on a fiber that can only be exposed in guanine-HCL I can not grow it on a slide. Also, I think I would need to bind the primary antibody (which is a polyclonal antibody) in the tris-Hcl then bind a secondary antibody with the gold, maybe after fixation. I am unsure which fixation technique would be best and what size of gold to use.

Any help you could give me would be greatly appreciated.
Thank you,
Erin Wagner
{mailto:e_wagner-at-pacific.edu} e_wagner-at-pacific.edu


From MicroscopyL-request-at-ns.microscopy.com Mon Jan 24 11:01:40 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 24 Jan 2005 11:07:05 -0600
Subject: [Microscopy] Perceptol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Go to the following URL for a discussion of Perceptol. One nugget is
that it is apparently very similar to Kodak D-23. Good luck.

http://largeformatphotography.info/lfforum/topic/500271.html

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








From MicroscopyL-request-at-ns.microscopy.com Mon Jan 24 18:11:23 2005



From: Delilah F. Wood :      wood-at-pw.usda.gov
Date: Mon, 24 Jan 2005 16:16:45 -0800
Subject: [Microscopy] MT-2 microtome service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anyone have any idea who might service my 1963 Sorvall Porter-Blum MT-2
microtome in the Berkeley-San Francisco area?

Thanks

Delilah Wood
USDA - ARS - WRRC
800 Buchanan St.
Albany, CA 94710




From MicroscopyL-request-at-ns.microscopy.com Mon Jan 24 19:09:15 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 24 Jan 2005 17:14:41 -0800
Subject: [Microscopy] Re: AskAMicroscopist : scanning electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Erin
It would be much easier to answer your questions if will be more specific:
what is the protein, which fibril - how your protein "embedded" into
fibril? Does fibril formed from the protein?

Basically, I think the best technique in your case would be TEM with
negative staining. Antibodies (AB) will not interact with specific antigen
in guanidine-HCl - this chemical is strong denaturing agent, it'll equally
denature (read deactivate) most of the ABs. If your protein somehow
present on fibril or part of the fibril- then ABs will react and you may
visualize the complex by negative staining. You may also enhance the
signal using gold-conjugated secondary ABs (10 nm would be OK). Basically,
SEM may be useless based on information you provided.

Have a good day, Sergey

At 07:49 AM 1/24/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Mon Jan 24 21:47:05 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 24 Jan 2005 19:52:05 -0800
Subject: [Microscopy] Re: Perceptol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Many years ago, this was treated as Kodak
Microdol-X. I used either one interchangeably.
They were pretty much the same to me. Temperature
and freshness were critical.

I figure that all b/w developers (other than
Microdol, et. al.) are variances of the basic
chemistry of b/w developers. Check out the
following links...

http://www.photococan.com/itm00487.htm


http://www.ilford.com/html/us_english/pdf/powder_dev.pdf

Ilford b/w film seemed to turn out better with
Microdol for either FP4+ or HP4+. But the difference
for results was the Zone treatment. In this case,
over exposure and underdevelopment resulted in
astonishing results. There is a huge amount of
discussion about this that lingers to this day.
The stated ISO of these films were not what I used.

If you want to see what Ilford FP4+ and HP5 do,
relative to fine art, navigate your way through

http://www.photoweb.net

You will find a large set of photo images that are
b/w and all shot using Ilford.

Why not underexpose and over develop? Well, if the
info is not on/in the neg, over developing is not going
to bring it out. This brings out the S curve about
which Ansel Adams made history. For SEM, this is
pre-collection LUT/gamma. No info at collection cannot
be made up later on. And blown out highlights cannot
be reduced to fact later on.

I guess that film is not dead yet. But the principles
slowly migrate to digital.

gary g.



At 09:07 AM 1/24/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Tue Jan 25 02:56:58 2005



From: vleppert-at-ucmerced.edu
Date: Tue, 25 Jan 2005 18:02:32 +0900
Subject: [Microscopy] SEM hard-soft specimen preparation question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues,

I am passing along a question regarding SEM sample preparation of a hard-soft specimen that I am hoping to get advice on. A colleague has implanted a collagen/titanium sample into bone and now removed it, and wishes to observe the interface of the bone with collagen, collagen with titanium, titanium with skin in the SEM. Any suggestions on sample preparation methods would be greatly appreciated. Further details of the sample are below.

It is 5 cm wide by 2 cm deep and the length is as follows:

/bone/collagen/titanium/skin
{2 cm} / {5cm} / {4cm} (titanium and skin)

Thank you,
Valerie
--------------------------------------------------------
Valerie J. Leppert, Assistant Professor
University of California, Merced
School of Engineering

Mailing Address:
P.O. Box 2039
Merced, CA 95344

Physical Address (for couriers/parcels):
4225 N. Hospital Road, Bldg 1200
Atwater, CA 95301

tel: (209) 724-4365
fax: (209) 724-2912
em: vleppert-at-ucmerced.edu



From MicroscopyL-request-at-ns.microscopy.com Tue Jan 25 03:02:12 2005



From: Delilah F. Wood :      wood-at-pw.usda.gov
Date: Mon, 24 Jan 2005 16:16:45 -0800
Subject: [Microscopy] MT-2 microtome service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Anyone have any idea who might service my 1963 Sorvall Porter-Blum MT-2
microtome in the Berkeley-San Francisco area?

Thanks

Delilah Wood
USDA - ARS - WRRC
800 Buchanan St.
Albany, CA 94710





From MicroscopyL-request-at-ns.microscopy.com Tue Jan 25 03:02:23 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 24 Jan 2005 19:52:05 -0800
Subject: [Microscopy] Re: Perceptol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Many years ago, this was treated as Kodak
Microdol-X. I used either one interchangeably.
They were pretty much the same to me. Temperature
and freshness were critical.

I figure that all b/w developers (other than
Microdol, et. al.) are variances of the basic
chemistry of b/w developers. Check out the
following links...

http://www.photococan.com/itm00487.htm


http://www.ilford.com/html/us_english/pdf/powder_dev.pdf

Ilford b/w film seemed to turn out better with
Microdol for either FP4+ or HP4+. But the difference
for results was the Zone treatment. In this case,
over exposure and underdevelopment resulted in
astonishing results. There is a huge amount of
discussion about this that lingers to this day.
The stated ISO of these films were not what I used.

If you want to see what Ilford FP4+ and HP5 do,
relative to fine art, navigate your way through

http://www.photoweb.net

You will find a large set of photo images that are
b/w and all shot using Ilford.

Why not underexpose and over develop? Well, if the
info is not on/in the neg, over developing is not going
to bring it out. This brings out the S curve about
which Ansel Adams made history. For SEM, this is
pre-collection LUT/gamma. No info at collection cannot
be made up later on. And blown out highlights cannot
be reduced to fact later on.

I guess that film is not dead yet. But the principles
slowly migrate to digital.

gary g.



At 09:07 AM 1/24/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Jan 25 03:03:17 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 24 Jan 2005 17:14:41 -0800
Subject: [Microscopy] Re: AskAMicroscopist : scanning electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Erin
It would be much easier to answer your questions if will be more specific:
what is the protein, which fibril - how your protein "embedded" into
fibril? Does fibril formed from the protein?

Basically, I think the best technique in your case would be TEM with
negative staining. Antibodies (AB) will not interact with specific antigen
in guanidine-HCl - this chemical is strong denaturing agent, it'll equally
denature (read deactivate) most of the ABs. If your protein somehow
present on fibril or part of the fibril- then ABs will react and you may
visualize the complex by negative staining. You may also enhance the
signal using gold-conjugated secondary ABs (10 nm would be OK). Basically,
SEM may be useless based on information you provided.

Have a good day, Sergey

At 07:49 AM 1/24/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu






From MicroscopyL-request-at-ns.microscopy.com Tue Jan 25 07:18:46 2005



From: germaine_g_boucher-at-groton.pfizer.com (by way of MicroscopyListserver)
Date: Tue, 25 Jan 2005 07:24:15 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Thanks for help on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (germaine_g_boucher-at-groton.pfizer.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, January 25, 2005 at 06:38:57
---------------------------------------------------------------------------

Email: germaine_g_boucher-at-groton.pfizer.com
Name: Germaine Boucher

Organization: Pfizer

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Thank you to everyone who responded to my question regarding reprocessing paraffin blocks for TEM. Your comments and suggestions have been helpful and enlightening.

Germaine Boucher
TEM lab
Pfizer Global Research and Development
(860)715-2708

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Jan 25 09:05:33 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Tue, 25 Jan 2005 09:12:06 -0600
Subject: [Microscopy] Re: AskAMicroscopist : scanning electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am not sure I fully understand your experimental design but I differ with
some of the comments that Sergey made. If you solublilize the protein in
guanine-HCl, then allow it to dry down on a substrate, you could gently was
with buffer, apply your primary antibody and follow that with a 5-10 nm
colloidal gold conjugated secondary antibody. Smaller gold colloids react
with higher efficiency than larger one (} 10 nm) so it is better to use a
smaller label and then enhance the gold size so that it was easier to see
in the SEM. I prefer gold enhanced gold (e.g., Nanoprobes kit) but silver
enhanced gold is also ok. We do this for tissues in the SEM all the time
with great results. Look at the gold with backscattered electron (BSE)
detectors and the surface topography with secondary electron detectors. I
am not sure why you need a fixation for an isolated fiber unless it was to
bind it to the substrate; this would increase retention but lower
immunoreactivity. Guanine-HCl treatment may destroy the reactivity of the
epitope with the antibody but, on the other hand, it may increase
reactivity similar to many antigen retrieval protocols.

At 09:49 AM 01/24/05, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Jan 25 14:45:32 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Tue, 25 Jan 2005 12:50:24 -0800
Subject: [Microscopy] Re: SEM hard-soft specimen preparation question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Valerie (Happy New Year)

For your colleague

First, how was the sample stabilized i.e. in what solution, when it was
removed and how is it being stored?
Also, is there still a lot of soft tissue remaining?

Next do you have access to an environmental SEM or critical point dryer?

If the tissue is already dried, then rehydrating it in a fixative and
standard biological SEM prep (given below), might help however there are
certain interfaces that were likely damaged if air drying already
occurred especially in the skin, as the pressure would be significant
from the drying.

SO, if the sample were already dried, I likely would try the following:

1. Examine the sample carefully in the stereo microscope and get
digital images and look for any possible drying artifacts (i.e. collapse
between the epidermal cells and the Ti. Check carefully also the Ti and
collagen. The collagen is fairly robust likely but the interface might
have pulled away.

2. If you have an environmental, or low pressure SEM, then examine it
directly in the SEM. This would be the easiest thing to do if the
instrument is available.

If no ESEM or low pressure SEM is available

A. Fix the sample in 3% glutaraldehyde (buffered with cacodylate at pH
7.2), 1 hr. Agitate in a rotator during fixation. If the skin is
thick, put in a vacuum chamber at room temp (i.e. a vacuum desiccator or
vacuum oven). Usually 20 mm Hg pressure is enough. Pull the vacuum
until all bubbles are gone from the vial, then turn off vac and let the
sample fix.
NOTE: Cacodylate (sodium salt) is usually a better buffer choice than
phosphate for SEM as phosphates often precipitate.

B. Wash sample in buffer, 2 or 3 times

C. Fix in 2% aq Osmium tetroxide (in hood) for 1 hr, RT

D. Dehydrate in 50, 75, 95% EtOH, 2-10 min changes and 2-30 min
changes in 100% EtOH

NOTE: If you have an industrial microwave, these times can be
considerably shortened.

E. Critical point dry (CPD) sample using carbon dioxide.

F. Coat with conductive material e.g. AuPd while rotating the sample
if possible.

If no CPD, then you could try putting it in xylene after the alcohol and
air dry or dry it directly after the alcohol. The surface tension will
be reduced somewhat when dried from an organic solvent, but of course
there will be some shrinkage. In reality even CPD gives shrinkage, but
usually less than drying from a solvent.

There is a method that uses HMDS (a silane), but don't think it is any
more commercially available. People used the drying from a solvent and
HMDS when they didn't have a CPD.

If the sample is already dried and you don't have a ESEM, then you may
want to simply coat it and look at it. It somewhat depends on the
number of samples you have. Once you coat it with a conductive coat, it
will become more difficult to fix, however it still could be fixed from
the bottom side if it isn't damaged from how it was mounted on the stub
for viewing.

If you have a current field emission gun SEM, then you may also be able
to use a low KV and get away with looking at it without coating. Many
of the new SEMs have a low pressure mode, which could be used to view
the sample also without coating.

The choice will be dependent on exactly what state the sample is in now,
i.e. wet, or already dried.

Let me know if you need more details.

Good Luck,
Judy


Judy Murphy, PhD
Microscopy & Imaging Consultant
Stockton, CA
murphyjudy-at-comcast.net




vleppert-at-ucmerced.edu wrote:

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From MicroscopyL-request-at-ns.microscopy.com Tue Jan 25 18:07:35 2005



From: psneeley-at-xmission.com (by way of MicroscopyListserver)
Date: Tue, 25 Jan 2005 18:13:18 -0600
Subject: [Microscopy] viaWWW: Manual for AO Series 2 & 4 Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (psneeley-at-xmission.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, January 25, 2005 at 08:19:18
---------------------------------------------------------------------------

Email: psneeley-at-xmission.com
Name: P.S. Neeley

Organization: None

Title-Subject: [Microscopy] [Filtered] Looking for an AO Series 2 & 4 Microscope Reference Manual

Question: use an American Optical Series 2 scope (160mm tube length) and have been unable to find a copy of the reference (user) manual. These scopes (Series 2 and 4) were manufactured in the 1950s after the heyday of the ëblackí AO models, and before the advent of the infinity corrected AO models (Series 10, 20, 110, etc.). It is possible that you may still have some of these scopes, and their manuals, kicking around the lab or classroom someplace.


I would like to obtain the reference manual if possible.


Ultimately, I would scan the reference manual and place it with the other AO manuals and catalogs available at: http://www.xmission.com/~psneeley/Personal/Microscope.htm for anyone to access and use.


Thank you for any information,


P.S. Neeley


---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 03:23:47 2005



From: lu kang :      luchunwang-at-hotmail.com
Date: Wed, 26 Jan 2005 16:07:56 +0000
Subject: [Microscopy] Brightness of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To everyone who replied about this question -

Thank you very much for the response. After careful consideration, my colleague, Dr. Hossein Hosseinkhani at ICYS, NIMS, Japan, tried the method suggested by Judy Murphy. He reports that it appears to have worked well and wanted me to convey his thanks for the suggestions.

Cheers,
Valerie

--------------------------------------------------------
Valerie J. Leppert, Assistant Professor
University of California, Merced
School of Engineering

Mailing Address:
P.O. Box 2039
Merced, CA 95344

Physical Address (for couriers/parcels):
4225 N. Hospital Road, Bldg 1200
Atwater, CA 95301

tel: (209) 724-4365
fax: (209) 724-2912
em: vleppert-at-ucmerced.edu

----- Original Message -----
} From: Judy Murphy {murphyjudy-at-comcast.net}

Microscopists,

Could anyone tell me how to measure and calculate the brightness and dose on
the fluorescent screen of a transmission electron microscope? We have the
Tecnai TEM.

Many thanks,

Lu




From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 12:02:42 2005



From: David Joswiak :      joswiak-at-astro.washington.edu
Date: Wed, 26 Jan 2005 10:07:52 -0800 (PST)
Subject: [Microscopy] staining of micromed sections to see organic carbon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is a question from a colleague (who is not on the list) regarding
staining of organic carbon.

Dave Joswiak
University of Washington


Hi,

I would be interested in any information regarding staining of organic
carbon prior to observing the sample in a TEM. I work with meteorites
and I am trying to distinguish the organic carbonaceous phases from the
not-organic carbon present in the samples.
I wonder if there exists any staining method to distinguish the organic
carbon from the rest(it does not need to be specific to a type of organic
carbon such as proteins, etc.). And I also wonder if there is a
way/procedure of staining directly the TEM grids containing microtomed
sections on them.

Thanks in advance.

--
Graciela Matrajt
Dept. of Astronomy







From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 14:03:15 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Wed, 26 Jan 2005 12:07:45 -0800
Subject: [Microscopy] Re: staining of micromed sections to see organic carbon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I do not know of a stain that would differentiate organic carbon from
not-organic carbon. But am responding to the comment

"And I also wonder if there is a
way/procedure of staining directly the TEM grids containing microtomed
sections on them."

Most of the standard TEM section stains attach to proteins, nucleic acids, or unsaturated lipids or to the Os which reduced those unsaturated bonds. There are several specialty stains as well which can attach to for instance, certain sugars.

However the standard staining method for TEM sections is usually the Reynolds uranyl acetate, lead citrate stain or some derivative of that. Lead citrate can be made from components (lead nitrate, sodium citrate) or using the compound lead citrate directly (e.g. Venables instant lead citrate stain). Generally I have found that making lead citrate from its components is more stable for a longer amount of time than using the "instant" lead citrate stains, however I have used both successfully. When I use the "instant" lead citrate, I usually do so by making it up fresh each time.

Reynolds, E.S., 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy, J. Cell Biol. 17, 208.
Venable, J. H. and Coggeshall, R., 1965. A simplified lead citrate stain for use in electron microscopy, J. Cell Biol 25, 407.
General reference
Lewis, P.R., and Knight, D.P., 1977. Staining methods for sectioned material, in "Practical Methods in Electron Microscopy", Volume 5, Part I,Audrey Glauert (Ed), Elsevier/North-Holland Press.

Below is one way to do the Reynolds uranyl acetate, lead citrate stain. It is likely that the formatting will not be retained in the text mode so in addition I am sending you off line a word document as an attachment which will retain the format. The below method indicates keeping the stains in syringes in a charged desiccator however you can also keep the stains in the refrigerator, just so they are sealed in something that keeps especially the lead citrate away from air and UA away from light.

Especially if you are interested in trying to find something that will differentiate a very minute difference, it is suggested to use deionized, glass distilled water. I have found when working with a variety os polymer staining methods that water that is purified by a resin column method (e.g. millipore) tends to bring along some of the resin on occasion which also nicely stains.

If you find such a stain that can differentiate what you are looking for, would appreciate knowing about it.

Thanks
Judy

Judy Murphy, PhD
Microscopy, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net

Uranyl Acetate and Reynolds Lead Citrate Staining
of Thin Sections for TEM:
Preparation and Use
Judy Murphy 050124

Staining of TEM thin sections involves a two step process, first staining with uranyl acetate and then with lead citrate. During the lead citrate staining, it is imperative to minimize the exposure of lead citrate with air to prevent precipitation on the sections of lead carbonate. To this end, NaOH pellets are used which removes the CO2 from the air. The more care that is taken to do the staining, the cleaner the sections will look.

NOTES:
• Preparation of Reynolds Lead Citrate stain takes approximately 30-40 minutes.
• Uranyl Acetate stain should be stored in a brown bottle to prevent reaction with light.
• Both stock stains are stored in the refrigerator at 4OC.
• Stains MUST be Millipore filtered just before use.
• Small amounts of the stains may be stored in 3cc syringes with the filters attached and placed in a charged desiccator.

1. Boil deionized, distilled water as follow:
NOTE: Freshly boiled water must be used to make the 0.02 N NaOH (if none available), water wash in wash bottle after staining, and to fill 10 ml water beakers at least 2 times. If new Lead Citrate stain needs to be made then it also needs to be made with freshly boiled water.
A. Prepare Beaker as follows:
a. Procure a clean 400-500 ml glass beaker.
CAUTION: Use a beaker 2-3 times larger than the volume of water to be boiled
b. Rinse the clean beaker with a small amount of deionized, distilled water.
B. Fill the rinsed beaker half full with deionized, distilled water.
C. Place beaker in middle of microwave and close the door.
E. Microwave water for 10 mins. Exact time depends on the microwave.
NOTE: Water should be allowed to boil about 5 minutes after it comes to a boil, which is about 10 min. in the microwave oven.
CAUTION: Do not leave boiling water unattended as water may boil over.
F. Using hot pad to protect skin, remove the beaker from the microwave.
G. Cover the beaker with half of a petri dish.
H. Allow the water to cool to room temperature.
NOTE: It is important that as little CO2 which is found in the air be allowed to enter back into the water. For that reason, water should be boiled shortly before use, leaving enough time only to cool the water.

2. Assemble stains and staining materials while waiting for water:
A. Freshly boiled and cooled deionized, distilled water (See Step 1 for preparation).
B. 2% Uranyl Acetate (aqueous) stain in labeled, clean syringe with FRESH Millipore filter attached (See Step 5A for preparation). This is stored in a charged desiccator specifically for the stains.
C. Reynold's Lead Citrate stain in labeled, clean syringe with FRESH Millipore filter attached (See Step 5B for preparation). This is stored in a charged desiccator specifically for the stains.
D. NaOH pellets in sealed container.
E. Wash bottle containing 0.02M NaOH (for rinse after Lead Citrate staining)
a. Measure out 180ml of the freshly boiled and cooled deionized, distilled water.
b. Pour water into a clean and rinsed wash bottle.
c. Add 2 NaOH pellets.
NOTE: Each pellet weighs approximately 0.07g
d. Mix solution well but do not cause CO2 to enter.
F. Wash bottle containing freshly cooled boiled distilled water.
G. Assemble the following necessary materials on a lined tray:
a. Three large glass Petri dishes (tops and bottoms). Line one Petri dish with a piece of filter paper indicated below.
b. One small Petri dish.
c. Three 10ml beakers.
d. Two waste beakers, one for the 0.02 N NaOH rinse waste and one for the water rinse waste.
e. Two squares of Parafilm cut to fit into one of the large Petri dish.
f. 2 pieces of filter paper, one for lining the Petri dish.
g. Filter paper arrows (fairly small arrows).
h. Clean EM tweezers, preferably locking tweezers, if available (may use small o-ring to lock regular tweezers).
i. Grid box containing sections to be stained with proper identification sheets.

3. Uranyl Acetate Staining:
A. Put grids that are to be stained within 20 min. in a covered Petri dish lined with a piece of filter paper.
B. Heat grids by putting them in the 52°C over for 15 minutes.
A. Fill the 10ml beakers 3/4 full with boiled deionized, distilled water.
B. Prepare Staining Petri dish as follows:
a. Place a square of parafilm in the bottom half of a glass petri dish.
CAUTION: Be sure that parafilm is flat.
b. Place drops of 2% UA on the paraflim layer in the petri dish, one per grid to be stained.
NOTE: Drops should be just larger than the grids.
C. Float each of the grids, singly, section-side down on a separate drop of stain and cover the Petri Dish.
D. Allow grids to stain for approximately 25-30 minutes.
E. Remove the grids one at a time and immediately rinse by quickly dipping 20 times into each of the three10ml beakers of fresh deionized, distilled water.
NOTE: Use a straight up and down motion.
F. Holding the grid in the tweezers, remove the water retained on the grid by touching filter paper arrows to the edge of the grid.
G. Place the grid on filter paper in a Petri dish.
H. Clean up of UA:
a. Dispose of stain in appropriate waste bottle under the fume hood.
b. Dispose of solid waste (filter paper arrows, parafilm etc.) in appropriate bio-hazardous waste container.

4. Lead Citrate Staining:
A. Fill the 10ml beakers approximately to 3 mm below the top, with freshly boiled and cooled deionized, distilled water.
B. Prepare Petri dishes as follows:
NOTE: Use a double Petri dish system i.e. small Petri dish top inside top and bottom of larger Petri dish.
a. Place a square of Parafilm in the bottom half of a glass Petri dish.
CAUTION: Be sure that parafilm is flat.
b. Place 4 NaOH pellets close to but NOT touching where the Lead Citrate droplets will be placed.
c. Place drops of Lead Citrate stain on the Parafilm layer in an area that can be covered by the small Petri dish, one per grid to be stained, near where the NaOH pellets are but NOT touching the pellets.
NOTE: Drops should be just larger than the grids.
d. Immediately put the small Petri dish top over the Parafilm area which has the Lead Citrate droplets and the NaOH pellets. Cover also the large Petri dish.
e. Allow it to sit about 5 min. to remove CO2 from the atmosphere surrounding the stain.
C. When ready to stain, quickly open the Petri dishes and float each of the grids, singly, section-side down on a separate drop of stain and quickly cover the Petri Dish.
NOTE: Open the Petri dishes only as far as necessary to minimize the amount of air (which contains CO2) that gets into the Petri dishes.
D. Allow grids to stain for appropriate time. This can vary between 5 to 20 min.
NOTE: Stain time will vary. Perform test for type of tissue and stain.
Staining Time Test: Start with 3 grids that all contain sections of the same thickness. These should be silver when viewed in the ultramicrotome. Stain one grid for 5 min, one for 10 min, and one for 15 min. with Lead Citrate. Uranyl acetate stain can all be 30 min. each.
E. Rinse the grids one at a time as follows:
a. Pick up the first grid and holding the grid with clean tweezers, gently run a stream of 0.02M NaOH (in wash bottle) over the grid and into a waste beaker.
NOTE: Start the stream of NaOH first and then bring the grid into the stream so it doesn't put extensive washing force on the grid.
b. Quickly, rinse the grid in the same manner with freshly boiled deionized, distilled water in a wash bottle.
c. Immediately rinse the grid by quickly dipping at least 20 times into each of the three 10ml beakers of fresh deionized, distilled distilled water.
NOTE: Use a straight up and down motion.
F. Remove liquid on tweezers and grid as follows:]
a. Holding the grid in the tweezers, remove the water retained in the tweezers by gently putting a paper arrow in between the tweezers coming from the non-pointed end downward.
NOTE: Anti-capillary tweezers do not require this step.
b. Still holding the grid in the tweezers, remove the water retained on the grid by carefully touching filter paper arrows to the edge of the grid.
G. Place the grid on filter paper in a covered Petri dish to dry. After a few minutes, the grid can be placed in a grid box indicating proper labeling information on the Grid Box Information Sheet.
H. Be sure to clean tweezers with clean water before picking up the next grid to be stained or before putting the tweezers away.
I. Clean up Lead Citrate waste as follows:
a. Dispose of used stain in appropriate waste bottle under the fume hood.
b. Dispose of solid waste (filter paper arrows, Parafilm etc.) in appropriate biohazardous waste container.

5. Preparation of Stains:
CAUTION: Wear Gloves when preparing stains.
A. 2% aqueous Uranyl Acetate Stain:
a. Measure 48ml deionized, distilled water into a clean100ml beaker.
b. Weigh out 1 gram Uranyl Acetate.
c. Add measured Uranyl Acetate to the deionized, distilled water in the beaker.
d. Bring volume up to 50ml with deionized, distilled water.
e. Stir solution on the magnetic stirrer to dissolve crystals.
f. Store stock stain in properly labeled brown bottle.
g. Prepare 3cc syringe of UA as follows:
i. Using a 3cc syringe, draw about 2ml of UA.
ii. Remove air from the syringe by holding the syringe straight up (needle on top) and slowly push on the plunger until the air is removed.
iii. Place a clean Sweeney filter holder loaded with a NEW Millipore filter on the end of the syringe.
iv. Label the syringe: "2% UA, Date and initials ".
v. Wrap the syringe with tin foil to protect the stain from light.
B. Reynold's Lead Citrate
CAUTION: Lead stains precipitate as white opaque carbonate grains on grids if any contact with C02 is made. Be careful not to breathe on stain drops or on grids while rinsing.
NOTE: Concentrated NaOH solutions should NOT be stored in glass-stoppered bottles or the stoppers will "freeze" and not be able to be removed.
a. Pour 30ml of the boiled and cooled deionized, distilled water into a 50ml volumetric flask.
b. Weigh out the following:
i. Lead Nitrate crystals: 1.33 g
ii. Sodium Citrate crystals: 1.76 g
c. Add the Lead Nitrate and Sodium Citrate crystals to the 30ml of water in the volumetric flask.
d. Mix the solution as follows:
i. Tightly seal the top of the volumetric flask with parafilm.
ii. Shake vigorously for 1 minute.
NOTE: Solution should be milky white.
iii. Allow to stand with occasional mixing for 30 minutes.
e. Add 1 N NaOH until cloudiness disappears as follows:
i. Add one drop of 1 N NaOH.
ii. Swirl solution until mixed.
iii. Repeat until cloudiness disappears.
g. pH solution to pH 12 using 10 N NaOH and bring the volume up to 50 ml with freshly boiled and cooled deionized, distilled water.
CAUTION: pH must be maintained above PH 12 or carbonate precipitate will form.
h. Store stain is stored in a tightly stoppered bottle.
i. Prepare 3cc syringe of Lead Citrate as follows:
i. Using a 3cc syringe, draw about 2ml of Lead Citrate.
ii. Remove air from the syringe by holding the syringe straight up (needle on top) and slowly push on the plunger until the air is removed.
iii. Place a clean Sweeney filter holder loaded with a NEW Millipore filter on the end of the syringe.
iv. Label the syringe: "Reynolds Lead Citrate, Date and initials ".
v. Place the loaded syringe in the charged desiccator.









David Joswiak wrote:

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From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 16:55:28 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Wed, 26 Jan 2005 14:59:54 -0800
Subject: [Microscopy] Re: Re: staining of micromed sections to see organic

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After receiving several off line comments from my friends about the fact
that Graciela Matrajt was working with meteorites not biological samples
and the staining methods I suggested wouldn't work, just want to add
that I responded to what I thought was a general question i.e. "And I
also wonder if there is a
way/procedure of staining directly the TEM grids containing microtomed
sections"
In retrospect, he perhaps meant microtomed meteorite samples and not
just any sample, in which case my response should be ignored. Sorry for
the wasted words on the listserver. It is hard to get good help anymore :)

General thoughts on meteorite sections and staining:
I have cut all sorts of materials i.e. metals, ceramics, ICs, polymers,
catalysts as well as biological samples, but never meteorites. Not
withstanding, if thin sections could be cut of meteorites, they may have
enough different inclusions etc. to form their own contrast by atomic
number and/or density difference. If it were more of a homogeneous
material, then perhaps the same stains we use for polymers might work
e.g. ruthenium or osmium tetroxide.
Linda Sawyer published a book on Microscopy of Polymers which was
updated in recent years.
I would think that getting nice thin sections of meteorites might be
more of a challenge than staining them.

Cheers
Judy Murphy

Judy Murphy wrote:

}
}
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}
} Hi,
} I do not know of a stain that would differentiate organic carbon from
} not-organic carbon. But am responding to the comment
}
} "And I also wonder if there is a
} way/procedure of staining directly the TEM grids containing microtomed
} sections on them."
}
} Most of the standard TEM section stains attach to proteins, nucleic
} acids, or unsaturated lipids or to the Os which reduced those
} unsaturated bonds. There are several specialty stains as well which
} can attach to for instance, certain sugars.
} However the standard staining method for TEM sections is usually the
} Reynolds uranyl acetate, lead citrate stain or some derivative of
} that. Lead citrate can be made from components (lead nitrate, sodium
} citrate) or using the compound lead citrate directly (e.g. Venables
} instant lead citrate stain). Generally I have found that making lead
} citrate from its components is more stable for a longer amount of time
} than using the "instant" lead citrate stains, however I have used both
} successfully. When I use the "instant" lead citrate, I usually do so
} by making it up fresh each time.
}
} Reynolds, E.S., 1963. The use of lead citrate at high pH as an
} electron-opaque stain in electron microscopy, J. Cell Biol. 17, 208.
} Venable, J. H. and Coggeshall, R., 1965. A simplified lead citrate
} stain for use in electron microscopy, J. Cell Biol 25, 407.
} General reference
} Lewis, P.R., and Knight, D.P., 1977. Staining methods for sectioned
} material, in "Practical Methods in Electron Microscopy", Volume 5,
} Part I,Audrey Glauert (Ed), Elsevier/North-Holland Press.
}
} Below is one way to do the Reynolds uranyl acetate, lead citrate
} stain. It is likely that the formatting will not be retained in the
} text mode so in addition I am sending you off line a word document as
} an attachment which will retain the format. The below method
} indicates keeping the stains in syringes in a charged desiccator
} however you can also keep the stains in the refrigerator, just so they
} are sealed in something that keeps especially the lead citrate away
} from air and UA away from light.
}
} Especially if you are interested in trying to find something that will
} differentiate a very minute difference, it is suggested to use
} deionized, glass distilled water. I have found when working with a
} variety os polymer staining methods that water that is purified by a
} resin column method (e.g. millipore) tends to bring along some of the
} resin on occasion which also nicely stains.
}
} If you find such a stain that can differentiate what you are looking
} for, would appreciate knowing about it.
}
} Thanks
} Judy
}
} Judy Murphy, PhD
} Microscopy, Imaging & Lab Design Consultant
} Stockton, CA 95219
} murphyjudy-at-comcast.net
}
} Uranyl Acetate and Reynolds Lead Citrate Staining of Thin Sections for
} TEM:
} Preparation and Use
} Judy Murphy 050124
}
} Staining of TEM thin sections involves a two step process, first
} staining with uranyl acetate and then with lead citrate. During the
} lead citrate staining, it is imperative to minimize the exposure of
} lead citrate with air to prevent precipitation on the sections of lead
} carbonate. To this end, NaOH pellets are used which removes the CO2
} from the air. The more care that is taken to do the staining, the
} cleaner the sections will look.
} NOTES: • Preparation of Reynolds Lead Citrate stain takes
} approximately 30-40 minutes. • Uranyl Acetate stain should be
} stored in a brown bottle to prevent reaction with light. • Both
} stock stains are stored in the refrigerator at 4OC. • Stains MUST
} be Millipore filtered just before use.
} • Small amounts of the stains may be stored in 3cc syringes with
} the filters attached and placed in a charged desiccator.
} 1. Boil deionized, distilled water as follow:
} NOTE: Freshly boiled water must be used to make the 0.02 N NaOH
} (if none available), water wash in wash bottle after staining, and to
} fill 10 ml water beakers at least 2 times. If new Lead Citrate stain
} needs to be made then it also needs to be made with freshly boiled water.
} A. Prepare Beaker as follows:
} a. Procure a clean 400-500 ml glass beaker.
} CAUTION: Use a beaker 2-3 times larger than the volume of
} water to be boiled
} b. Rinse the clean beaker with a small amount of deionized,
} distilled water.
} B. Fill the rinsed beaker half full with deionized, distilled
} water.
} C. Place beaker in middle of microwave and close the door.
} E. Microwave water for 10 mins. Exact time depends on the
} microwave. NOTE: Water should be allowed to boil about 5
} minutes after it comes to a boil, which is about 10 min. in the
} microwave oven.
} CAUTION: Do not leave boiling water unattended as water may
} boil over.
} F. Using hot pad to protect skin, remove the beaker from the
} microwave.
} G. Cover the beaker with half of a petri dish.
} H. Allow the water to cool to room temperature.
} NOTE: It is important that as little CO2 which is found in
} the air be allowed to enter back into the water. For that reason,
} water should be boiled shortly before use, leaving enough time only to
} cool the water.
}
} 2. Assemble stains and staining materials while waiting for water:
} A. Freshly boiled and cooled deionized, distilled water (See
} Step 1 for preparation).
} B. 2% Uranyl Acetate (aqueous) stain in labeled, clean syringe
} with FRESH Millipore filter attached (See Step 5A for preparation).
} This is stored in a charged desiccator specifically for the stains.
} C. Reynold's Lead Citrate stain in labeled, clean syringe with
} FRESH Millipore filter attached (See Step 5B for preparation). This is
} stored in a charged desiccator specifically for the stains.
} D. NaOH pellets in sealed container.
} E. Wash bottle containing 0.02M NaOH (for rinse after Lead
} Citrate staining)
} a. Measure out 180ml of the freshly boiled and cooled
} deionized, distilled water.
} b. Pour water into a clean and rinsed wash bottle.
} c. Add 2 NaOH pellets.
} NOTE: Each pellet weighs approximately 0.07g
} d. Mix solution well but do not cause CO2 to enter.
} F. Wash bottle containing freshly cooled boiled distilled water.
} G. Assemble the following necessary materials on a lined tray:
} a. Three large glass Petri dishes (tops and bottoms). Line
} one Petri dish with a piece of filter paper indicated below.
} b. One small Petri dish.
} c. Three 10ml beakers.
} d. Two waste beakers, one for the 0.02 N NaOH rinse waste
} and one for the water rinse waste.
} e. Two squares of Parafilm cut to fit into one of the large
} Petri dish.
} f. 2 pieces of filter paper, one for lining the Petri dish.
} g. Filter paper arrows (fairly small arrows).
} h. Clean EM tweezers, preferably locking tweezers, if
} available (may use small o-ring to lock regular tweezers).
} i. Grid box containing sections to be stained with proper
} identification sheets.
}
} 3. Uranyl Acetate Staining:
} A. Put grids that are to be stained within 20 min. in a covered
} Petri dish lined with a piece of filter paper.
} B. Heat grids by putting them in the 52°C over for 15 minutes.
} A. Fill the 10ml beakers 3/4 full with boiled deionized,
} distilled water.
} B. Prepare Staining Petri dish as follows:
} a. Place a square of parafilm in the bottom half of a glass
} petri dish.
} CAUTION: Be sure that parafilm is flat.
} b. Place drops of 2% UA on the paraflim layer in the petri
} dish, one per grid to be stained.
} NOTE: Drops should be just larger than the grids.
} C. Float each of the grids, singly, section-side down on a
} separate drop of stain and cover the Petri Dish.
} D. Allow grids to stain for approximately 25-30 minutes.
} E. Remove the grids one at a time and immediately rinse by quickly
} dipping 20 times into each of the three10ml beakers of fresh
} deionized, distilled water. NOTE: Use a straight up and down
} motion.
} F. Holding the grid in the tweezers, remove the water retained on
} the grid by touching filter paper arrows to the edge of the grid.
} G. Place the grid on filter paper in a Petri dish.
} H. Clean up of UA:
} a. Dispose of stain in appropriate waste bottle under the
} fume hood.
} b. Dispose of solid waste (filter paper arrows, parafilm
} etc.) in appropriate bio-hazardous waste container.
}
} 4. Lead Citrate Staining:
} A. Fill the 10ml beakers approximately to 3 mm below the top,
} with freshly boiled and cooled deionized, distilled water.
} B. Prepare Petri dishes as follows:
} NOTE: Use a double Petri dish system i.e. small Petri dish
} top inside top and bottom of larger Petri dish.
} a. Place a square of Parafilm in the bottom half of a glass
} Petri dish.
} CAUTION: Be sure that parafilm is flat.
} b. Place 4 NaOH pellets close to but NOT touching where the
} Lead Citrate droplets will be placed.
} c. Place drops of Lead Citrate stain on the Parafilm layer
} in an area that can be covered by the small Petri dish, one per grid
} to be stained, near where the NaOH pellets are but NOT touching the
} pellets.
} NOTE: Drops should be just larger than the grids.
} d. Immediately put the small Petri dish top over the
} Parafilm area which has the Lead Citrate droplets and the NaOH
} pellets. Cover also the large Petri dish. e. Allow it to
} sit about 5 min. to remove CO2 from the atmosphere surrounding the stain.
} C. When ready to stain, quickly open the Petri dishes and float
} each of the grids, singly, section-side down on a separate drop of
} stain and quickly cover the Petri Dish.
} NOTE: Open the Petri dishes only as far as necessary to
} minimize the amount of air (which contains CO2) that gets into the
} Petri dishes.
} D. Allow grids to stain for appropriate time. This can vary
} between 5 to 20 min.
} NOTE: Stain time will vary. Perform test for type of tissue
} and stain.
} Staining Time Test: Start with 3 grids that all contain
} sections of the same thickness. These should be silver when viewed in
} the ultramicrotome. Stain one grid for 5 min, one for 10 min, and one
} for 15 min. with Lead Citrate. Uranyl acetate stain can all be 30
} min. each. E. Rinse the grids one at a time as follows:
} a. Pick up the first grid and holding the grid with clean
} tweezers, gently run a stream of 0.02M NaOH (in wash bottle) over the
} grid and into a waste beaker.
} NOTE: Start the stream of NaOH first and then bring the
} grid into the stream so it doesn't put extensive washing force on the
} grid.
} b. Quickly, rinse the grid in the same manner with freshly
} boiled deionized, distilled water in a wash bottle.
} c. Immediately rinse the grid by quickly dipping at least
} 20 times into each of the three 10ml beakers of fresh deionized,
} distilled distilled water.
} NOTE: Use a straight up and down motion.
} F. Remove liquid on tweezers and grid as follows:]
} a. Holding the grid in the tweezers, remove the water
} retained in the tweezers by gently putting a paper arrow in between
} the tweezers coming from the non-pointed end downward.
} NOTE: Anti-capillary tweezers do not require this step.
} b. Still holding the grid in the tweezers, remove the water
} retained on the grid by carefully touching filter paper arrows to the
} edge of the grid.
} G. Place the grid on filter paper in a covered Petri dish to
} dry. After a few minutes, the grid can be placed in a grid box
} indicating proper labeling information on the Grid Box Information Sheet.
} H. Be sure to clean tweezers with clean water before picking up
} the next grid to be stained or before putting the tweezers away.
} I. Clean up Lead Citrate waste as follows:
} a. Dispose of used stain in appropriate waste bottle under
} the fume hood.
} b. Dispose of solid waste (filter paper arrows, Parafilm
} etc.) in appropriate biohazardous waste container.
}
} 5. Preparation of Stains:
} CAUTION: Wear Gloves when preparing stains.
} A. 2% aqueous Uranyl Acetate Stain:
} a. Measure 48ml deionized, distilled water into a
} clean100ml beaker.
} b. Weigh out 1 gram Uranyl Acetate.
} c. Add measured Uranyl Acetate to the deionized, distilled
} water in the beaker.
} d. Bring volume up to 50ml with deionized, distilled water.
} e. Stir solution on the magnetic stirrer to dissolve crystals.
} f. Store stock stain in properly labeled brown bottle.
} g. Prepare 3cc syringe of UA as follows:
} i. Using a 3cc syringe, draw about 2ml of UA.
} ii. Remove air from the syringe by holding the syringe
} straight up (needle on top) and slowly push on the plunger until the
} air is removed.
} iii. Place a clean Sweeney filter holder loaded with a NEW
} Millipore filter on the end of the syringe.
} iv. Label the syringe: "2% UA, Date and initials ".
} v. Wrap the syringe with tin foil to protect the stain from
} light.
} B. Reynold's Lead Citrate
} CAUTION: Lead stains precipitate as white opaque carbonate
} grains on grids if any contact with C02 is made. Be careful not to
} breathe on stain drops or on grids while rinsing.
} NOTE: Concentrated NaOH solutions should NOT be stored in
} glass-stoppered bottles or the stoppers will "freeze" and not be able
} to be removed.
} a. Pour 30ml of the boiled and cooled deionized, distilled
} water into a 50ml volumetric flask.
} b. Weigh out the following:
} i. Lead Nitrate crystals: 1.33 g
} ii. Sodium Citrate crystals: 1.76 g
} c. Add the Lead Nitrate and Sodium Citrate crystals to the
} 30ml of water in the volumetric flask.
} d. Mix the solution as follows:
} i. Tightly seal the top of the volumetric flask with
} parafilm.
} ii. Shake vigorously for 1 minute.
} NOTE: Solution should be milky white.
} iii. Allow to stand with occasional mixing for 30 minutes.
} e. Add 1 N NaOH until cloudiness disappears as follows:
} i. Add one drop of 1 N NaOH.
} ii. Swirl solution until mixed.
} iii. Repeat until cloudiness disappears.
} g. pH solution to pH 12 using 10 N NaOH and bring the
} volume up to 50 ml with freshly boiled and cooled deionized, distilled
} water.
} CAUTION: pH must be maintained above PH 12 or carbonate
} precipitate will form.
} h. Store stain is stored in a tightly stoppered bottle.
} i. Prepare 3cc syringe of Lead Citrate as follows:
} i. Using a 3cc syringe, draw about 2ml of Lead Citrate.
} ii. Remove air from the syringe by holding the syringe
} straight up (needle on top) and slowly push on the plunger until the
} air is removed.
} iii. Place a clean Sweeney filter holder loaded with a NEW
} Millipore filter on the end of the syringe.
} iv. Label the syringe: "Reynolds Lead Citrate, Date and
} initials ".
} v. Place the loaded syringe in the charged desiccator.
}
}
}
}
}
}
}
}
}
} David Joswiak wrote:
}
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
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} } -------------------------------------------------------------------------------
} }
} }
} } Below is a question from a colleague (who is not on the list) regarding
} } staining of organic carbon.
} }
} } Dave Joswiak
} } University of Washington
} }
} }
} } Hi,
} }
} } I would be interested in any information regarding staining of organic
} } carbon prior to observing the sample in a TEM. I work with meteorites
} } and I am trying to distinguish the organic carbonaceous phases from the
} } not-organic carbon present in the samples.
} } I wonder if there exists any staining method to distinguish the organic
} } carbon from the rest(it does not need to be specific to a type of
} } organic
} } carbon such as proteins, etc.). And I also wonder if there is a
} } way/procedure of staining directly the TEM grids containing microtomed
} } sections on them.
} }
} } Thanks in advance.
} }
} }
} }
}
}


From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 17:34:35 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 26 Jan 2005 15:53:03 -0800
Subject: [Microscopy] Re: Brightness of TEM

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On Jan 26, 2005, at 8:07 AM, lu kang wrote:

} Could anyone tell me how to measure and calculate the brightness and
} dose on the fluorescent screen of a transmission electron microscope?
} We have the Tecnai TEM.
}
Dear Lu,
There are several ways to do this, but for the most accurate results,
the screen should be calibrated with a suitable Faraday cup. When we
first measured screen current with the utility in the low dose set-up,
it did not agree with the measurement from the screen current
utility--you may or may not have access to that utility, but your FEI
service person does. Subsequently, we calibrated both measurements,
and we now can be confident that either will give us a true reading.
We have continued to check the CCD sensitivity, and, since that has not
changed measurably, we can now use the CCD to determine the desired
dose by setting the beam parameters to give us the number of counts per
channel that corresponds to the number of electrons per nm^2.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 18:36:54 2005



From: armstrong30-at-llnl.gov (by way of MicroscopyListserver)
Date: Wed, 26 Jan 2005 18:42:35 -0600
Subject: [Microscopy] viaWWW: EM cooling stage questions

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (armstrong30-at-llnl.gov) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, January 26, 2005 at 13:27:33
---------------------------------------------------------------------------

Email: armstrong30-at-llnl.gov
Name: Mike Armstrong

Organization: Lawrence Livermore Nat'l Labs

Title-Subject: [Microscopy] [Filtered] EM cooling stage questions and shopping (buyer)

Question: Hi!

I am interested in doing TEM on frozen (but not necessarily cooled to liquid nitrogen temperatures) samples in a JEOL 2000FX TEM. I have two sets of questions:

1) Does anybody out there have a cooling stage for this microscope that I could beg/borrow/rent/buy? Even if you have a lead that I could check out, it would be great if you could let me know.

2) Does anyone know of a Peltier cooled stage for TEM (for this microscope) that's a reasonable price? How hard do you think it would be to build such a thing? I only need to go to sub-freezing, not super cold, and it seems like this might be a cheaper alternative.

I'm new to EM, so if you have any other suggestions, they would be helpful. I'm kind of scraping to get this for as little as possible. If anybody can help me, I'd really appreciate it. This is for a high risk/payoff experiment and you will certainly get a mention (at least) in some acknowledgements if you can help me out.

Cheerios,

Mike

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 18:44:06 2005



From: sschul-at-vet.ksu.edu (by way of MicroscopyListserver)
Date: Wed, 26 Jan 2005 18:49:31 -0600
Subject: [Microscopy] viaWWW: help on SEM/TEM of TICKS

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sschul-at-vet.ksu.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, January 26, 2005 at 15:01:11
---------------------------------------------------------------------------

Email: sschul-at-vet.ksu.edu
Name: Sarah

Organization: Kansas State University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I am going to do a project that involves ticks. The ticks will feed on the dogs and then drop off, once they drop off and mature to adults I would like to do SEM and TEM on them to determine the location of the bacteria with in the tick itself. I believe that I will do critical point dry. I was wondering if you could help me out with a protocol for how to specifically fix the ticks to ensure that they are being processed correctly. Thanks

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From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 18:50:41 2005



From: James Pawley :      jbpawley-at-wisc.edu
Date: Wed, 26 Jan 2005 18:56:06 -0600
Subject: [Microscopy] First AND ONLY announcement: Tenth International Live-cell Course.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

Hard to believe we are at Year 10 already. Thank you all for your
support over the years.

As the list is now enforcing the One-Announcement-per-Course rule,
don't expect further announcements.

Up-to-date information can always be found at the Course WWW site:
www.3dcourse.ubc.ca

Cheers,

Jim P.


_______________________________

Tenth Annual INTERNATIONAL 12-Day Short Course on 3D Microscopy of
Living Cells, June 11 - 23, 2005 (Pre-course: June 11)

Ninth, Post-course Workshop on 3D Image Processing,
June 25 -27, 2005

Organized by Prof. James Pawley, (University of Wisconsin-Madison)

in association with the Departments of Pharmacology and Physiology
and the Brain Research Centre,
University of British Columbia, Vancouver, BC, Canada

DATES

Applications must be received by Tuesday, March 15, 2005
Deposit due Friday,
April 15, 2005
Registration 5:00 - 7:00 PM Saturday, June 11, 2005
First Lecture 7:30 PM Saturday, June 11, 2005
Live-cell Course ends, noon Thursday, June 23, 2005
3D Image Processing Course, Saturday, June 25 -
Monday, 27, 2005


APPLICATIONS DUE BY MARCH 15, 2005

APPLICATIONS
Applicants must complete a questionnaire on the web to assess
knowledge level, field of interest and proposed personal, live-cell,
project. Enrollment will be limited to about 32 participants (exact
number depends on number of 3D Systems available). Selection will be
made on the basis of background and perceived need. Those without
previous LM experience will be provided with access to basic texts to
read before the course begins. Application forms requesting
information on field of interest and level of experience may be
down-loaded from the WWW site at

www.3dcourse.ubc.ca/application.htm , or obtained from:

Prof. James B. Pawley, Ph.
608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
250 N. Mills Street, Madison, WI, 53706 JBPAWLEY-at-WISC.EDU

Additional information is available from:
www.3dcourse.ubc.ca/brochure.htm and links.

We expect to have at least 11, 3D microscope workstations for student
use and there will be an international faculty of 20.

Application deadlines:

Application forms must be received for screening by March 15, 2005.
Successful applicants will be notified by April 1, and a deposit of
50% must be received by April 15, 2005. In general, refunds of the
deposit will only be possible if someone on the waiting list can take
the place but this has not been a problem in previous years. The
remaining balance is due before Registration.


Pre-Course Tuition (1/2 day Basic Optical principles)
$100 (US)
3D Live-cell Course Tuition (includes lunches, snacks, 3 dinners):
$2,650 (US)
Workshop Tuition (includes lunches and snacks):
$1,000 (US)

Room/board about $40/day (US) depending on room type.

I hope that this includes all of the information that you need, but
if not, please get back to me.

--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU
"A scientist is not one who can answer questions but one who can
question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 19:24:11 2005



From: sdublin-at-emory.edu (by way of Ask-A-Microscopist)
Date: Wed, 26 Jan 2005 19:29:52 -0600
Subject: [Microscopy] AskAMicroscopist: Trying to make my own carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sdublin-at-emory.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, January 26, 2005 at 15:57:22
---------------------------------------------------------------------------

Email: sdublin-at-emory.edu
Name: Steven Dublin

Organization: Emory University

Education: Graduate College

Location: Atlanta, GA

Question: I am a grad student trying to make my own carbon coated grids. I have no problem depositing a carbon film on a glass slide and floating the film on water. Next,I drop the grids on the film. My problem occurs when I swipe the grids off the surface of the water with a glass slide. The residual water on the slide wrinkles and tears the film, so I am left with an uneven, broken film across the surface of the grid.

Does anyone have advice on how to get the grids out of the water without tearing the film? What about grid glue? Is this required?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 20:19:23 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 26 Jan 2005 18:37:54 -0800
Subject: [Microscopy] Re: viaWWW: EM cooling stage questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 26, 2005, at 4:42 PM, by way of MicroscopyListserver wrote:

} I am interested in doing TEM on frozen (but not necessarily cooled to
} liquid nitrogen temperatures) samples in a JEOL 2000FX TEM. I have two
} sets of questions:
}
} 1) Does anybody out there have a cooling stage for this microscope
} that I could beg/borrow/rent/buy? Even if you have a lead that I could
} check out, it would be great if you could let me know.
}
} 2) Does anyone know of a Peltier cooled stage for TEM (for this
} microscope) that's a reasonable price? How hard do you think it would
} be to build such a thing? I only need to go to sub-freezing, not super
} cold, and it seems like this might be a cheaper alternative.
}
} I'm new to EM, so if you have any other suggestions, they would be
} helpful. I'm kind of scraping to get this for as little as possible.
} If anybody can help me, I'd really appreciate it. This is for a high
} risk/payoff experiment and you will certainly get a mention (at least)
} in some acknowledgements if you can help me out.
}
Dear Mike,
I can't help you regarding the JEOL 2000FX, but an additional
possibility for a cooled stage is to beg/borrow/rent/buy a LN2 stage
and fill it with a dry ice-ethanol slurry (which a JEOL rep can tell
you if this mixture could cause damage). Ice-salt-water can also be
used for barely sub-freezing temps, but I would rinse thoroughly
afterward. If you want to build a Peltier-cooled stage, you just need
the appropriate thermal contact to which one can attach the Peltiers;
however, that might be easier said than machined. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 20:48:30 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Wed, 26 Jan 2005 18:53:21 -0800
Subject: [Microscopy] Re: AskAMicroscopist: Trying to make my own carbon

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steven,

Instead of picking up the carbon from the water surface with a glass
slide all at once, it may be possible to break up the carbon on the
water surface slightly and pick up a small 3 mm piece individually with
each grid from underneath.

Usually for carboned only grids for virus examination, we would
evaporate carbon onto freshly cleaved mica pieces (available from EM
suppliers). We then floated the carbon films onto the surface of the
water. Using freshly acetone cleaned grids, we put one grid at a time
underneath the pieces of carbon and picked it up. Freshly cleaning
them, would lower the surface tension when the grid went into the
water. We generally used 400 mesh Cu grids to support the films.

"Does anyone have advice on how to get the grids out of the water without tearing the film? What about grid glue? Is this required? "


I have used grid glue routinely for picking up sections on naked grids
but have never found it necessary for carbon only grids.

Good luck,
Judy


Judy Murphy, PhD
Microscopy, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net










by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 21:20:57 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 26 Jan 2005 19:26:13 -0800
Subject: [Microscopy] Re: AskAMicroscopist: Trying to make my own

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Steven
Try to use piece of Parafilm (instead glass) to pick up the
grids. Parafilm should be larger than carbon film. You need completely
immerse Parafilm into the water and than remove it quickly. Grids should be
clean to stick to the carbon. Depends from carbon thickness, you need to
use grids of corresponding mesh: As thinner carbon, higher mesh should
be. Carbon, which is slightly brownish (so you could see carbon by naked
eye) may be mounted on 400-mesh grids. The alternative (there are so many
ways) way is to put plastic film on the grids (which is easy) first, then
evaporate carbon and then dissolve plastic (it would "glue" carbon to the
grid). You may also leave plastic on the grid. Generally, carbon do not
stick well to the naked grids, so people used plastic support film or
"holey film". You may also "pre-treat" grids with plastic (a drop of very
diluted plastic solution per grid, dry before use) before mounting the
carbon. I hope it helps. Have a great day, Sergey

At 05:29 PM 1/26/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Wed Jan 26 23:00:33 2005



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Thu, 27 Jan 2005 15:50:22 +0100
Subject: [Microscopy] Calibration of objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It has been a long time since I've made carbon coated grids, but one
of the approaches we used was to wrap a piece of coarse copper mesh
around some wire (coat hanger would do) to create a support that we
could place under water. We then dunked the grids through the water
onto this support, and then floated off the carbon film onto the
surface of the water. We were then able to manipulate the grid
holder under the carbon film, and pick up the whole thing through the
water.

The simple method, of course, was to make thin formvar coated grids,
and evaporate some carbon on to them. Does your work preclude this
approach?

Joel


Date sent: Wed, 26 Jan 2005 18:53:21 -0800
} From: Judy Murphy {murphyjudy-at-comcast.net}
To: by way of Ask-A-Microscopist {sdublin-at-emory.edu}
Copies to: microscopy-at-microscopy.com

Hi all,

I've been trying to calibrate the objectives of a brightfield microscope
from 2,5x to 100x to its best, but still I have an average deviation of
3,9% if I compare the same object measured on all magnifications. So if
I measure something with the 2,5x, it appears longer then with the 40x
objective.
Is 3,9% acceptable as deviation and if not, what is? Is there a general
rule or a paper about this?
Thanks in advance,

Sven Terclavers



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 10:01:57 2005



From: Dale Batchelor :      dale_batchelor-at-ncsu.edu
Date: Thu, 27 Jan 2005 11:10:19 -0500
Subject: [Microscopy] AFM Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Announcing a 5 day intensive AFM short course entitled "AFM and Other
Scanned Probe Microscopies". It is being taught at N.C. State
University in
Raleigh, NC by Prof. Phil Russell, Dr. Joe Griffith and others from June
13 -17, 2005.

This one-week short course on atomic force microscopy has evolved from
the
numerous Scanned Probe Microscopy courses developed and taught by Prof.
Russell over the past 2 decades. It is designed for technicians,
scientists,
engineers and researchers. The course includes lectures and laboratories
with hands-on time using a variety of scanning probe microscope (SPM)
systems.

For more information go to www.ncsu.edu/aif/afmcourse

Dale Batchelor, Ph.D.
Course Coordinator
N.C. State University
Analytical Instrumentation Facility
EGRC room 318C
Campus Box 7531
Raleigh, NC 27695
Office 919-515-3841
FAX 919-515-6965
E-Mail dale_batchelor-at-ncsu.edu
Website www.ncsu.edu/aif



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 15:41:07 2005



From: James Mohr :      james.mohr-at-onsemi.com
Date: Thu, 27 Jan 2005 14:47:42 -0700
Subject: [Microscopy] SEM FIB - Technician Job Opportunity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ON Semiconductor has an immediate technician job opening in the Phoenix,
AZ, Failure Analysis Lab for an SEM / FIB / metallographic prep
Technician.

Please see Job Posting 688AZ at www.onsemi.com for a full description.

You can apply at the website
http://www.careerexchange.com/cejobs/DetailOnsemi.asp?onsemi688AZ

or contact me for more information.
Thank you,
James Mohr
James.mohr-at-onsemi.com
602-244-3483




From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 16:12:24 2005



From: George_Munzing-at-engelhard.com
Date: Thu, 27 Jan 2005 17:20:02 -0500
Subject: [Microscopy] Hair Analysis References

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

I am looking to obtain some references pertaining to the analysis of human
hair, both morphologic and spectroscopic. I'm trying to get my feet wet and
need a place to start. Does anyone have particular recommendations?

Any help is greatly appreciated.

Thanks in advance!

George R. Munzing Jr.
Engelhard Corporation
Strategic Technologies Group
Iselin, NJ 08830
TEL: 732-205-7030
FAX: 732-494-3283
e-mail: george.munzing-at-engelhard.com



From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 17:32:53 2005



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Thu, 27 Jan 2005 17:37:37 -0600
Subject: [Microscopy] Hair Analysis References

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A spectroscopic reference to a study of Beethoven's hair (finding
evidence of lead poisoning):

http://www.aps.anl.gov/News/APS_News/2000/20001017.htm

Jeffrey A. Fortner, Ph.D.
Chemical Engineering Division
Argonne National Laboratory
Argonne, IL 60439
fortner(at)cmt.anl.gov



-----Original Message-----
} From: George_Munzing-at-Engelhard.com [mailto:George_Munzing-at-Engelhard.com]

Sent: Thursday, January 27, 2005 4:20 PM
To: Microscopy-at-MSA.Microscopy.Com

Hello All,

I am looking to obtain some references pertaining to the analysis of
human hair, both morphologic and spectroscopic. I'm trying to get my
feet wet and need a place to start. Does anyone have particular
recommendations?

Any help is greatly appreciated.

Thanks in advance!

George R. Munzing Jr.
Engelhard Corporation
Strategic Technologies Group
Iselin, NJ 08830
TEL: 732-205-7030
FAX: 732-494-3283
e-mail: george.munzing-at-engelhard.com





From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 18:01:07 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Thu, 27 Jan 2005 16:05:48 -0800
Subject: [Microscopy] Re: Hair Analysis References

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi George,

Been dabbling in forensic microscopy for the last 5 yrs with a high
school forensics program and have been using the following for
microscopy of human hair

Atlas of Human Hair: Microscopic Characteristics
ISBN: 0-8493-8134-7

take care,
Judy

Judy Murphy, PhD
Microscopy, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net




George_Munzing-at-engelhard.com wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 19:21:21 2005



From: gunkelrl-at-slu.edu (by way of MicroscopyListserver)
Date: Thu, 27 Jan 2005 19:26:47 -0600
Subject: [Microscopy] viaWWW: manual for Cryo-cut cryostat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (gunkelrl-at-slu.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, January 27, 2005 at 12:06:03
---------------------------------------------------------------------------

Email: gunkelrl-at-slu.edu
Name: Rebecca

Organization: St. Louis University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Does anyone have a operators manual to an old (very old) Cryo-cut cryostat?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 19:21:50 2005



From: kaprelyants-at-yahoo.com (by way of MicroscopyListserver)
Date: Thu, 27 Jan 2005 19:27:30 -0600
Subject: [Microscopy] viaWWW: looking for info on Leo Scopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kaprelyants-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, January 27, 2005 at 15:24:07
---------------------------------------------------------------------------

Email: kaprelyants-at-yahoo.com
Name: Alex Kaprelyants, Ph.D.

Organization: Childrens Hospital, Columbus

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I am looking for info about:

LEO 920A TEM
LEO SM940 SEM.

Thank you.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 20:09:31 2005



From: Elaine Humphrey :      ech-at-interchange.ubc.ca
Date: Thu, 27 Jan 2005 18:14:52 -0800
Subject: [Microscopy] Re: Hair Analysis References

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hey Judy
I have this book. I think George should be warned though that while
the book is useful, it is only about a quarter of an inch thick and
small, and cost about $107 Canadian for 83 pages. It was a shock when
I got it.. I expected at least a tome of some sort.
Elaine

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca


From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 20:33:32 2005



From: :      Colin.Veitch-at-csiro.au
Date: Fri, 28 Jan 2005 12:05:08 +1100
Subject: [Microscopy] RE: Hair Analysis References

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi George,

A couple to start you off with.... There may be more in a day or so
when I get a chance to go through some more....

Jones LN. Clinics in Dermatology, 2001;19:95-103.

Rogers GE. Electron microscope studies of hair and wool, Ann NY Acad Sci
1959;83:469-485.

Hallegot P, Corcuff P. High-spatial-resolution maps of sulphur from
human hair sections: An EELS study. J. Micrsc 1993;172:131-136.

Jones LN, Cholewa M, Kaplin IJ, et al. Elemental distributions in
keratin/follicle sections. Proc Eighth Int Wool Textile Conf.
Christchurch NZ 1990;1:246-255.

Rogers GE, Reis PL, et al., editors. The biology of wool and hair.
London: Chapam and Hall, 1989.

Cheers

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.




From MicroscopyL-request-at-ns.microscopy.com Thu Jan 27 22:18:57 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Thu, 27 Jan 2005 20:23:53 -0800 (PST)
Subject: [Microscopy] Re: Hair Analysis References

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

is this evian or naive of me, but have you tried med
line? maybe pubmed.
john
--- George_Munzing-at-engelhard.com wrote:

}
}
}
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} Microscopy Society of America
} To Subscribe/Unsubscribe --
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} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Hello All,
}
} I am looking to obtain some references pertaining to
} the analysis of human
} hair, both morphologic and spectroscopic. I'm trying
} to get my feet wet and
} need a place to start. Does anyone have particular
} recommendations?
}
} Any help is greatly appreciated.
}
} Thanks in advance!
}
} George R. Munzing Jr.
} Engelhard Corporation
} Strategic Technologies Group
} Iselin, NJ 08830
} TEL: 732-205-7030
} FAX: 732-494-3283
} e-mail: george.munzing-at-engelhard.com
}
}
}




__________________________________
Do you Yahoo!?
All your favorites on one personal page – Try My Yahoo!
http://my.yahoo.com


From MicroscopyL-request-at-ns.microscopy.com Fri Jan 28 01:38:02 2005



From: Gordon Couger :      gcc-at-couger.com
Date: Fri, 28 Jan 2005 01:42:17 -0600
Subject: [Microscopy] Re: Re: Hair Analysis References

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


George,

Variations on a goggle search www.google.com
"human hair" preparation microscope
look promising try replacing perpareion with other terms like
microtome, embedding, spurs, resin, etc and you should find a
lot of things.

On McCrone's Modern Microscopy publication
http://www.modernmicroscopy.com/main.asp
A Microscopical Study of Exotic Animal Hair: Part 1
by Kristen D. Skraba, McCrone Associates, Westmont, IL.
Might be of interstest even thought it is about animal hair it
shows a few tecniques and methods. The biligoriphy should help
you some.

Check your local library if it is not a major reserch universty
it is unlikely they will have anything but you can search the
Libary of Congress at:
http://catalog.loc.gov/cgi-bin/Pwebrecon.cgi?DB=local&PAGE=First
and use inter library loan to get anything that intrests you.
It's not the instant access that the web give but it a geat
service partiulary for obscure papers from all over the world.

Good luck
Gordon
Gordon Couger

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org






} } Hello All,
} }
} } I am looking to obtain some references pertaining to
} } the analysis of human
} } hair, both morphologic and spectroscopic. I'm trying
} } to get my feet wet and
} } need a place to start. Does anyone have particular
} } recommendations?
} }
} } Any help is greatly appreciated.
} }
} } Thanks in advance!
} }
} } George R. Munzing Jr.
} } Engelhard Corporation
} } Strategic Technologies Group
} } Iselin, NJ 08830
} } TEL: 732-205-7030
} } FAX: 732-494-3283
} } e-mail: george.munzing-at-engelhard.com
} }
} }
} }
}
}
}
}
}
} __________________________________
} Do you Yahoo!?
} All your favorites on one personal page – Try My Yahoo!
} http://my.yahoo.com
}
}





From MicroscopyL-request-at-ns.microscopy.com Fri Jan 28 07:21:32 2005



From: spagosullamiavaligia-at-libero.it (by way of Ask-A-Microscopist)
Date: Fri, 28 Jan 2005 07:27:13 -0600
Subject: [Microscopy] AskAMicroscopist: microscope incubation systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (spagosullamiavaligia-at-libero.it) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, January 28, 2005 at 05:35:21
---------------------------------------------------------------------------

Email: spagosullamiavaligia-at-libero.it
Name: Marco Cattaneo

Organization: University of Cosenza

Education: Graduate College

Location: Cosenza, Italy

Question: Dear Sir/Madam,
i need to keep cells alive under the microscope for several hours. I realize that exist two microscope incubation systems: the box surrounding the microscope and the chamber fitting on the microscope stage. Could you please tell me which are the meaningful differences between these two microscope incubation methods? Is it true that the chamber fitting on the stage causes a focus drift? Which are the most reliable brand for these systems (obviously with the best quality/price ratio)?

Thanks in advance

Best regards,
Marco

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jan 28 09:09:04 2005



From: jerry.w.ball-at-exxonmobil.com
Date: Fri, 28 Jan 2005 09:13:58 -0600
Subject: [Microscopy] Cryotomy- Automated Sample Facing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is there an automated cryotome that can face multiple samples with little
human intervention?

Jerry W. Ball
Advanced Characterization
Product Technology
BTEC West Complex
ExxonMobil Chemical Company
5200 Bayway Dr. 77520-2101
Phone: 281-834-1718
Fax: 281-834-1793
E-Mail: jerry.w.ball-at-exxonmobil.com



From MicroscopyL-request-at-ns.microscopy.com Fri Jan 28 11:29:44 2005



From: Richard Harris :      rjharris-at-uwo.ca
Date: Fri, 28 Jan 2005 12:33:31 -0500
Subject: [Microscopy] Sectioning woody plant stems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers
I have a researcher who would like to create cross sections of 2 - 3 year
old pine sapling stems.
The sections do not need to be terribly thin (50 - 100 micrometers) but he
would like to have the surface as smooth as possible without embedding the
tissue (he is a physiologist interested in chloroplasts). My background is
with animal tissue so I'm at a bit of a loss in how to advise him. Any
suggestions would be gratefully accepted.
Thanks in advance...

Richard Harris
Laboratory Supervisor
Microscopy, Imaging and Analysis
Department of Biology
University of Western Ontario
London Ontario CANADA
N6A 5B7
Ph. 519-661-2111 ext. 86780
Fax 519-661-3935



From MicroscopyL-request-at-ns.microscopy.com Fri Jan 28 14:07:23 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 28 Jan 2005 15:12:16 -0500
Subject: [Microscopy] Re: Sectioning woody plant stems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard,
If you have access to a vibratome than use that to cut the sections. Also,
with some practice you may be able to cut free-hand sections that are thin
enough for your purposes.

Alternatively you can buy little hand microtomes from major science or
school supply companies. These cost about $100 I think and have a sample
chuck that is raised by a little micrometer screw assembly through a flat
platform. You insert your stem and then gradually raise it in height. Then
you slide a long razor blade across the piece using the platform as a
support. It is really a simple but very useful device.

If we have a tissue that is too soft or small to hold well in the chuck
then we just hollow out a piece of carrot and place the twig, etc inside.
This will clamp well and hold the tissue well to get minimal crushing and
distortion during cutting.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 1/28/05 12:33 PM, "Richard Harris" {rjharris-at-uwo.ca} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Dear Listers
} I have a researcher who would like to create cross sections of 2 - 3 year
} old pine sapling stems.
} The sections do not need to be terribly thin (50 - 100 micrometers) but he
} would like to have the surface as smooth as possible without embedding the
} tissue (he is a physiologist interested in chloroplasts). My background is
} with animal tissue so I'm at a bit of a loss in how to advise him. Any
} suggestions would be gratefully accepted.
} Thanks in advance...
}
} Richard Harris
} Laboratory Supervisor
} Microscopy, Imaging and Analysis
} Department of Biology
} University of Western Ontario
} London Ontario CANADA
} N6A 5B7
} Ph. 519-661-2111 ext. 86780
} Fax 519-661-3935
}
}




From MicroscopyL-request-at-ns.microscopy.com Fri Jan 28 17:10:31 2005



From: Kim Rensing :      krensing-at-interchange.ubc.ca
Date: Fri, 28 Jan 2005 15:17:24 -0800
Subject: [Microscopy] Re: Sectioning woody plant stems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would recommend a sliding microtome (aka sledge microtome) if you can
find one. The other possibilities are a cryostat or hand sections made
with a double-edged razor blade. The hand sections are OK if he doesn't
need consistency.
Kim

{} {} {} {} {} {} {}
Kim Rensing PhD
Bio-Imaging Facility, UBC
6270 University Boulevard
Vancouver, BC V6T 1Z4
{} {} {} {} {} {} {}

On Friday, January 28, 2005, at 09:33 AM, Richard Harris wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Dear Listers
} I have a researcher who would like to create cross sections of 2 - 3
} year
} old pine sapling stems.
} The sections do not need to be terribly thin (50 - 100 micrometers)
} but he
} would like to have the surface as smooth as possible without embedding
} the
} tissue (he is a physiologist interested in chloroplasts). My
} background is
} with animal tissue so I'm at a bit of a loss in how to advise him. Any
} suggestions would be gratefully accepted.
} Thanks in advance...
}
} Richard Harris
}



From MicroscopyL-request-at-ns.microscopy.com Fri Jan 28 21:59:09 2005



From: svanhorn-at-notes.cc.sunysb.edu (by way of MicroscopyListserver)
Date: Fri, 28 Jan 2005 22:04:49 -0600
Subject: [Microscopy] viaWWW: to cut adipose tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (svanhorn-at-notes.cc.sunysb.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, January 28, 2005 at 14:23:09
---------------------------------------------------------------------------

Email: svanhorn-at-notes.cc.sunysb.edu
Name: Sue Van Horn

Organization: SUNY-at-StonyBrook

Title-Subject: [Microscopy] [Filtered] Adipose tissue

Question: I am trying to cut adipose tissue for lightlevel.am having a horrible time trying cryostat sections..any suggestions???
thanks
sue

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Jan 29 14:44:16 2005



From: Gordon Couger :      gcc-at-couger.com
Date: Sat, 29 Jan 2005 14:48:45 -0600
Subject: [Microscopy] Re: Re: Sectioning woody plant stems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A good sharp wood plane iron or wide wood chisel sharpened as
you would a microtome knife are much better than the straight
razor that usually comes with them. Cementing a glass microscope
slide on each side of the hole in the face of the microtome will
help too. Trim the glass slides so they don't cause a danger you
the operator and use cutting edges made from good steel not the
imported stuff. You may have to find and old chisel or plane to
get a good piece of steel but the good modern brands should be
good enough if you don't have and old on laying around.

Razor blades and straight razors are not stiff enough to good
sections from soft tissue let alone hard stuff.

Try cutting with a slicing motion instead of with the blade at
90 degrees to the direction of travel.

Good luck
Gordon
Gordon Couger

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org

Debby Sherman wrote:
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Richard,
} If you have access to a vibratome than use that to cut the sections. Also,
} with some practice you may be able to cut free-hand sections that are thin
} enough for your purposes.
}
} Alternatively you can buy little hand microtomes from major science or
} school supply companies. These cost about $100 I think and have a sample
} chuck that is raised by a little micrometer screw assembly through a flat
} platform. You insert your stem and then gradually raise it in height. Then
} you slide a long razor blade across the piece using the platform as a
} support. It is really a simple but very useful device.
}
} If we have a tissue that is too soft or small to hold well in the chuck
} then we just hollow out a piece of carrot and place the twig, etc inside.
} This will clamp well and hold the tissue well to get minimal crushing and
} distortion during cutting.
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
} On 1/28/05 12:33 PM, "Richard Harris" {rjharris-at-uwo.ca} wrote:
}
}
} }
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Sat Jan 29 16:08:46 2005



From: Damian Neuberger :      neuberger1234-at-comcast.net
Date: Sat, 29 Jan 2005 16:14:07 -0600
Subject: [Microscopy] RE: Re: Re: Sectioning woody plant stems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard,

Having cut a lot of wood and especially pine, maybe I can add a comment or
two here. The requirement "smooth as possible" is a little vague; it would
help to know how these sections are going to be examined i.e. optical, SEM,
AFM?

1. From my experience, I've found the best way to section this material is
using a regular microtome knife; disposable microtome blades just don't hold
up well enough. A good well sharpened microtome knife will do a great job
and stand up to the hard xylem elements.

2. Depending on the diameter of the sapling stems, they may have to be
surrounded (not embedded) with something to make them more rigid. Options
range from Paraplast to Epoxy depending on how hard the stem material is.

3. When sectioning, it helps to soak the block in ice water (or warm water
depending on the material used in #2) to soften the woody portions of the
stem.

4. Fixing Pinus tissue is a whole other problem!

Good luck on what can be a challenging project

Damian Neuberger



} Dear Listers
} I have a researcher who would like to create cross sections of 2 - 3 year
} old pine sapling stems.
} The sections do not need to be terribly thin (50 - 100 micrometers) but he
} would like to have the surface as smooth as possible without embedding the
} tissue (he is a physiologist interested in chloroplasts). My background is
} with animal tissue so I'm at a bit of a loss in how to advise him. Any
} suggestions would be gratefully accepted.
} Thanks in advance...
}
} Richard Harris
} Laboratory Supervisor
} Microscopy, Imaging and Analysis
} Department of Biology
} University of Western Ontario
} London Ontario CANADA
} N6A 5B7
} Ph. 519-661-2111 ext. 86780
} Fax 519-661-3935





From MicroscopyL-request-at-ns.microscopy.com Sun Jan 30 16:00:29 2005



From: Rosemary White :      Rosemary.White-at-csiro.au
Date: Mon, 31 Jan 2005 09:09:20 +1100
Subject: [Microscopy] Re: Sectioning woody plant stems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Richard,

If your researcher wants to use fresh wood sections, the sliding or sledge
microtome suggested by Kim Rensing will do a great job, as long as your
knives are sharp. We found that the disposable blades are not stiff enough
for doing this on the sledge microtome, you need to use the large metal
blades. A colleague recently sectioned 2-year old poplar stems this way.
We're lucky that a local histologist will sharpen the blades for us.

If you use a hand microtome, a non-flexible blade, something like a
cut-throat razor, is probably necessary to get reasonably consistent
sections. With hard tissue, the flexible blades chatter on the sledge
microtome (on our one, anyway), and tend to flex and scoop out the middle of
the tissue on a hand microtome.

For support material, we use high density foam, the type used for insulating
refrigerators. We have a large slab of this and just cut a small piece
approximately to shape to fit around the tissue, in the same way we get
students to shape carrot pieces around tissue for hand sectioning.
Everything from shoot apical meristems to cores of 50-year old trees have
been sectioned this way on the sledge microtome.

Good luck!
cheers,
Rosemary



Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia

} From: Richard Harris {rjharris-at-uwo.ca}
} Date: Fri, 28 Jan 2005 12:33:31 -0500
} To: MSA Listserver {microscopy-at-MSA.microscopy.com}
} Subject: [Microscopy] Sectioning woody plant stems
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Dear Listers
} I have a researcher who would like to create cross sections of 2 - 3 year
} old pine sapling stems.
} The sections do not need to be terribly thin (50 - 100 micrometers) but he
} would like to have the surface as smooth as possible without embedding the
} tissue (he is a physiologist interested in chloroplasts). My background is
} with animal tissue so I'm at a bit of a loss in how to advise him. Any
} suggestions would be gratefully accepted.
} Thanks in advance...
}
} Richard Harris
} Laboratory Supervisor
} Microscopy, Imaging and Analysis
} Department of Biology
} University of Western Ontario
} London Ontario CANADA
} N6A 5B7
} Ph. 519-661-2111 ext. 86780
} Fax 519-661-3935
}
}


From MicroscopyL-request-at-ns.microscopy.com Sun Jan 30 16:28:12 2005



From: Jacob.blumenthal-at-weizmann.ac.il (by way of MicroscopyListserver)
Date: Sun, 30 Jan 2005 16:36:34 -0600
Subject: [Microscopy] viaWWW: negative staining of mRNA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Jacob.blumenthal-at-weizmann.ac.il) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Sunday, January 30, 2005 at 04:03:29
---------------------------------------------------------------------------

Email: Jacob.blumenthal-at-weizmann.ac.il
Name: Jacob Blumenthal

Organization: weizmann institute of science

Title-Subject: [Microscopy] [Filtered] MListserver: negative staining of mRNA

Question: Hello, I would like to know if it possible to see mRNA molecule after the ribosomes came off of it using negative staining with uranyl acetate? It appears as a long thin string (not a straight one).
Another question is what is diameter of a single ribosome as it apperas in em using negative staining? And if you can always see the forming peptides coming out from each ribosome?
I am working on eukaryotic cells.
Thanks,

Jacob Blumenthal

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Jan 31 10:25:53 2005



From: George_Munzing-at-engelhard.com
Date: Mon, 31 Jan 2005 11:36:31 -0500
Subject: [Microscopy] Hair analysis references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you all for your replies and suggestions. I think I have enough to
keep me busy for quite a while.

Regards

George R. Munzing Jr.
Engelhard Corporation
Strategic Technologies Group
Iselin, NJ 08830
TEL: 732-205-7030
FAX: 732-494-3283
e-mail: george.munzing-at-engelhard.com



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 31 14:05:10 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 01 Feb 2005 09:13:29 +1300
Subject: [Microscopy] Need replacement for dead Coolpix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year

For the last year or so we have been using a Coolpix 4500 interfaced to a petrographic
microscope, yielding considerable user satisfaction.

We have the WPI Adaptor Kit, P/N 501384, which has a 28mm male thread that screws
directly into the filter thread on the end of the lens of the Coolpix. Although the
interfaceability of the Coolpix is a bit primitive, as all images are stored only on the card
memory and have to be transferred to a computer either via the USB cable (slow) or by
removing the card (fiddly), the mechanical coupling is firm and good, the optics work
well.

The camera has now died, and I have to plan its replacement in case it turns out to be
not repairable.

The Coolpix 4500 has been discontinued, and my local Nikon agents advise that there
is no comparable Nikon replacement. As I understand it, the reasons for the
widespread use of the 4500 for this application include the fact that it has a thread on
the end of the lens for easy attachment of the adaptor, and that the end of the lens
doesn't rotate or otherwise move as the camera changes its focus or zoom.

The WPI kit includes adaptors to step up to match camera female threads of 37mm and
43mm, although I can see that the more adaptors that are used, the less rigid the whole
setup may become.

What alternative cameras are currently being successfully used out there?

Preferably with filter threads of 28, 37, or 43mm so that I can continue to use my WPI
kit.


cheers and tia

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 31 14:28:31 2005



From: pgrover :      pgrover-at-bilbo.bio.purdue.edu
Date: Mon, 31 Jan 2005 15:37:02 -0500
Subject: [Microscopy] how to do a survey; Sorvall TC-2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone have a manual for a Sorvall TC-2 tissue sectioner they could
copy & send me?

On a completely different note, does anyone know how one could go about
conducting a very brief (two or three multiple choice questions),
non-commercial survey of career scientists? I'd like to make it so the
responses are anonymous, thereby increasing likelihood of candid answers.

Paul :0)

----------------------------------------------------------------------
"Keep your eyes slightly wide and blank. Show no interest or excitement."
- Dr. Miles Bennell
Invasion of the Body Snatchers (1956)






From MicroscopyL-request-at-ns.microscopy.com Mon Jan 31 15:59:26 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 31 Jan 2005 14:07:53 -0800
Subject: [Microscopy] Re: viaWWW: negative staining of mRNA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Jackob
It's possible to see the poly-ribosomes, when many individual ribosomes
attached to single mRNA molecule. You may find many of articles already
published on this matter. I don't remember did they use negative staining
or shadowing, I would think: both. As far as I remember, the ribosomes
looks like spherical particles without any details. The single ribosome
visualization is another story. Yes, you could see easily individual
ribosome by negative staining and size for 70S E.coli ribosome is about 2.6
nm (approx. diameter). Eucariotic ribosome may be a little bigger. If you
are talking about mRNA attached to the ribosome - it's completely different
story. You need to keep in mind that in order to function, ribosome needs
at least two molecules of tRNA, mRNA and a bunch of "factors" - all this
stuff attached to the ribosome making the picture very complicated. In
addition, mRNA will never be like "linear structure" under physiological
conditions: it would form compact structure, which is very difficult to
made "straight"... synthesized peptide would act in similar way - you
never will see peptide as a linear structure: it is immediately folded at
the exit (even before). So, finally, you have the ribosomal particle
surrounded with bunch of other molecules. All of them are sort of
globular and interact with each other... There are million articles
published on this matter. You may take a look on works by Joahim (spell?)
Frank - he did 3D reconstruction of bacterial (?) ribosome with different
factors, mRNA etc... Do google search on "ribosome structure Frank"... Good
luck! Sergey

At 04:36 PM 1/30/2005 -0600, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Jan 31 17:10:55 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Mon, 31 Jan 2005 17:17:57 -0600
Subject: [Microscopy] Re: how to do a survey; Sorvall TC-2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Paul,

I can't help on the microtome, but MME has been conducting surveys for 15+ years. Contact me off-line and we'll see what we can do.

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.



At 02:37 PM 1/31/2005, pgrover wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Mon Jan 31 18:30:39 2005



From: hgabrisc-at-uno.edu (by way of MicroscopyListserver)
Date: Mon, 31 Jan 2005 18:38:59 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: post doctoral position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (hgabrisc-at-uno.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, January 31, 2005 at 17:06:42
---------------------------------------------------------------------------

Email: hgabrisc-at-uno.edu
Name: Heike Gabrisch

Organization: Universit of New Orleans

Title-Subject: [Microscopy] [Filtered] MListserver: post doctoral position

Question: We have an immediate opening for a post-doctoral scholar. Her/his duties include the characterization of transition metal compounds by electron diffraction techniques. Transition metal compounds are used as intercalation compounds in rechargeable Li-ion batteries. The position requires hands-on experience in transmission microscopy - a background in crystallography is a plus. Contact : Heike Gabrisch (hgabrisc-at-uno.edu)

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Jan 31 19:49:25 2005



From: Gordon Couger :      gcc-at-couger.com
Date: Mon, 31 Jan 2005 19:57:08 -0600
Subject: [Microscopy] Re: Need replacement for dead Coolpix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Richie,

This has been a hot topic on the Yahoo microscope list.
http://groups.yahoo.com/group/Microscope/

I have been told by a reliable source a CoolPix 5000 and even
the 5400 can be use with UR-E6 for the 5000 and ER-U11 for the
5400 a part that cost less than 20 dollars in place of the CP
600-4500.

There is no other cameras that will work the optics the CoolPix
600-4500 I now of with out custom parts.

If the 4500 was satisfactory just buy a couple of used ones from
some one with good reputation on ebay. They are pretty sturdy
cameras. If you have problems with artifacts
http://www.couger.com/microscope/shootout/shootout.html
when you use the zoom function on the camera you might want to
consider buying a CoolPix 990 or go the the 5,000 that doesn't
have this problems.


Make sure if you buy a new camera you buy with the right to
return it if doesn't work to suit you.

Gordon
Gordon Couger

I collect links on information related to light microscopes.
http://www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org


Ritchie Sims wrote:
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Happy New Year
}
} For the last year or so we have been using a Coolpix 4500 interfaced to a petrographic
} microscope, yielding considerable user satisfaction.
}
} We have the WPI Adaptor Kit, P/N 501384, which has a 28mm male thread that screws
} directly into the filter thread on the end of the lens of the Coolpix. Although the
} interfaceability of the Coolpix is a bit primitive, as all images are stored only on the card
} memory and have to be transferred to a computer either via the USB cable (slow) or by
} removing the card (fiddly), the mechanical coupling is firm and good, the optics work
} well.
}
} The camera has now died, and I have to plan its replacement in case it turns out to be
} not repairable.
}
} The Coolpix 4500 has been discontinued, and my local Nikon agents advise that there
} is no comparable Nikon replacement. As I understand it, the reasons for the
} widespread use of the 4500 for this application include the fact that it has a thread on
} the end of the lens for easy attachment of the adaptor, and that the end of the lens
} doesn't rotate or otherwise move as the camera changes its focus or zoom.
}
} The WPI kit includes adaptors to step up to match camera female threads of 37mm and
} 43mm, although I can see that the more adaptors that are used, the less rigid the whole
} setup may become.
}
} What alternative cameras are currently being successfully used out there?
}
} Preferably with filter threads of 28, 37, or 43mm so that I can continue to use my WPI
} kit.
}
}
} cheers and tia
}
} rtch
}
} --
} Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
} Microanalyst Fax : 64 9 3737435
} Department of Geology email : r.sims-at-auckland.ac.nz
} The University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 01:11:15 2005



From: reinhard rachel :      reinhard.rachel-at-biologie.uni-regensburg.de
Date: Tue, 1 Feb 2005 08:19:06 +0100 (MET)
Subject: [Microscopy] TEM - negative staining of mRNA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

BTW: the ribosome is about 25 to 30 nm in diameter,
depending on the projection, but not 2.6 nm.
Just a typing error, I assume.
Kind regards,
Reinhard
-----------------
PD Dr. Reinhard Rachel
Universitaet Regensburg
Microbiology
Universitaetsstr. 31
D-93053 Regensburg - Germany




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 08:04:32 2005



From: Somayyeh_kheiri-at-yahoo.com (by way of MicroscopyListserver)
Date: Tue, 1 Feb 2005 08:13:09 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: clearing method for leaves

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Somayyeh_kheiri-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, February 1, 2005 at 06:45:57
---------------------------------------------------------------------------

Email: Somayyeh_kheiri-at-yahoo.com
Name: Somayyeh

Title-Subject: [Microscopy] [Filtered] about clearing method

Question: Hello Dear All

I am researching anatomical aspects of the Verbascum.

I was trying to provide freehand sections of the leaves
unfortunately the sections were thick and did not have
good focus.

So I heard of clearing method .
Can the clearing method be used after sectioning the samples by hand to give good focus?

I appreciate your kind reply


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 09:48:13 2005



From: McFaddin, Wade :      Wade.McFaddin-at-nextekinc.com (by way of
Date: Tue, 1 Feb 2005 09:46:51 -0600
Subject: [Microscopy] viaWWW: Position Open: Senior Analytical Engineer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nextek Inc. has an immediate opening for the following failure/materials
analysis engineer position. If interested, please fill out an application
and send your resume to our human resources department (see our web page
link at the end of the job posting).

Feel free to contact me if you have any questions.
Thanks,
Wade McFaddin
Nextek Inc.
256-772-1995 ext.1064

JOB: Senior Analytical Engineer SHIFT: 1st
Job No: AS001-04

Responsibilities:

Report directly to the Manager of Analytical Service or Vice President of
Product Assurance.

Perform material failure analyses such as FTIR analysis, thermal analysis,
scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS)
analysis, C-mode scanning acoustic microscopy analysis, cross section
evaluation of printed circuit boards (PCB) and printed
circuit board assemblies (PCBA). Support in-house PCBA manufacturing
process and external customer requirements.

Evaluate final results of analysis, prepare and present reports outlining
the outcome of analysis, and make recommendations for actions necessary to
achieve desired results.

Job Requirements:
Communication, presentation, and technical writing skills to effectively
communicate to and from a diverse group such as the customer, Nextek
employees and suppliers. Electronic manufacturing experience required.

Experience/Education/Training Requirements:
B.S. degree in Engineering, Materials Science or Science or equivalent
5 - 10 years of electronic material laboratory experience

If you meet the requirements for this job and would like to be considered,
please complete the

Nextek Employment Application on our website at www.nextekinc.com and submit
to Human Resources.



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 13:58:53 2005



From: David_Bell-at-Millipore.com
Date: Tue, 1 Feb 2005 14:56:23 -0500
Subject: [Microscopy] SEM Pictures of Mycoplasm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

I have a colleague who is interested in finding SEM micrographs of
mycoplasm (note: not mycoplasma!). We are trying to take pictures of it,
but find ourselves confounded by apparent detritus in the growth media,
such that images of the "fresh" media alone look very similar to the
organisms that have been cultivated. If anyone has done work with this
organism, and could provide us with one or two pictures to use as a
reality check, it would be greatly appreciated!

Sincerely,

David




David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 14:29:47 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 01 Feb 2005 12:28:24 -0800
Subject: [Microscopy] Re: TEM - negative staining of mRNA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Yes, I am sorry, it was a typo: 70S E.coli ribosome is about 26 nm in
} coronal projection. Thanks Reinhard for correction!

} Sergey
}
} BTW: the ribosome is about 25 to 30 nm in diameter,
} depending on the projection, but not 2.6 nm.
} Just a typing error, I assume.
} Kind regards,
} Reinhard
} -----------------
} PD Dr. Reinhard Rachel
} Universitaet Regensburg
} Microbiology
} Universitaetsstr. 31
} D-93053 Regensburg - Germany



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 18:22:44 2005



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Wed, 2 Feb 2005 11:20:39 +1100
Subject: [Microscopy] embedding tiny specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I've been given some vanishingly small specimens to embed for TEM.
They're peels from the surface of a retina - at most several cells
thick and usually less than 1x2 mm in area. They are also made mostly
of basement membrane, with a few whole cells and some cell fragments,
so not only are they almost invisible to start with, but they remain
invisible even after osmium. Pinning them down won't work - they're too
small. I've embedded some once using a dissecting microscope, but it
was a lot of work and even so I lost 2 of the 6. I thought of embedding
first in agar, but then I'll end up with an invisible specimen
somewhere in the resin, which will take longer find by serial
sectioning than I'm prepared to spend.

Any ideas would be most welcome!

Diana



Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 20:17:55 2005



From: Paul Webster :      pwebster-at-hei.org
Date: Tue, 01 Feb 2005 18:14:46 -0800
Subject: [Microscopy] Re: embedding tiny specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I once had specimens so small the final pellet had an area of only one or
two grid holes (cryosectioned too!) so I think I may be able to help here.

After lots of trials I eventually put the specimens in a thin polypropylene
centrifuge tube (about 0.5ml total volume), re-suspended them in 10% gelatin
(warm) and pelleted them down to the bottom of the tube. At the time the
tubes had sharp pointy ends so the pellet of cell fragments went to the very
tip if I used a horizontal rotor. I am not sure that these tubes are
available any more.

Anyway, after the gelatin had gelled, I cut off the tip of the tube, which
is fairly easy if the tube is made of polypropylene, and embedded it using a
routine protocol. The gelatin turned dark and the tip was easily visible so
I was able to orientate the block to cut the tip without problem.

Since then, I have been working with slightly larger amounts of cell
fragments and have found that if you centrifuge the specimens down in warm
gelatin in a regular Eppendorf tube, the pellet always deposits at the same
place. I orientate myself in a fixed angle rotor (45 degrees) by putting the
lid hinge outwards. I look for the pellet at the bottom of the tube under
the hinge. Always wait for the gelatin to gel and cut out the pellet.
Sometimes you cut where you think the pellet is. The smaller the gelatin
block the easier you will be able to section.

If you were to be doing this for immunolocalization then agarose (2%) may be
better. You can visualize the block after embedding by adding some color
(alcian blue, dextran blue etc) to make it visible in the polymerized resin.

I know that some people say you shouldn't use gelatin because of its high
affinity for water, but embedding protocols using gelatin have worked for me
for many years.

Regards,

Paul Webster.



Paul Webster, Ph.D.
Director
Ahmanson Advanced EM and Imaging Center
House Ear Institute
2100 West Third Street
Los Angeles
CA 90057-1922
Phone: 213 273 8026
Fax: 213 13 739
E-mail: pwebster-at-hei.org




On 2/1/05 4:20 PM, "Diana van Driel" {dianavd-at-eye.usyd.edu.au} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Hi all,
}
} I've been given some vanishingly small specimens to embed for TEM.
} They're peels from the surface of a retina - at most several cells
} thick and usually less than 1x2 mm in area. They are also made mostly
} of basement membrane, with a few whole cells and some cell fragments,
} so not only are they almost invisible to start with, but they remain
} invisible even after osmium. Pinning them down won't work - they're too
} small. I've embedded some once using a dissecting microscope, but it
} was a lot of work and even so I lost 2 of the 6. I thought of embedding
} first in agar, but then I'll end up with an invisible specimen
} somewhere in the resin, which will take longer find by serial
} sectioning than I'm prepared to spend.
}
} Any ideas would be most welcome!
}
} Diana
}
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney, NSW
} AUSTRALIA 2001
}
} Phone 61 2 93827278
} Mobile 0423 151614
} FAX 61 2 93827318
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 20:41:08 2005



From: subramss-at-email.uc.edu (by way of MicroscopyListserver)
Date: Tue, 1 Feb 2005 20:39:47 -0600
Subject: [Microscopy] viaWWW: Phosphorus contamination in the ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (subramss-at-email.uc.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, February 1, 2005 at 10:54:28
---------------------------------------------------------------------------

Email: subramss-at-email.uc.edu
Name: Jimble

Organization: University of Cincinnati

Title-Subject: [Microscopy] [Filtered] Phosphorus contamination in the ESEM

Question: Dear friends and elders,

I was wondering if someone would be able to help me track down a phosphorus contamination issue which I am observing in my ESEM when I use a hotstage. I am giving details of the instrument and conditions below. The materials I am observing are high purity oxides and should have but trace if any contamination of phosphorus.

Instrument: FEI XL-30 ESEM FEG with Diffusion pump backed a Mechanical pump.
The system does not have any in-line filters or residual gas analyzers.
Oils: Edwards Ultra 19 (Mechanical)
Santovac 5 (Diffusion pump)

Mode of operation : Gaseous mode 2 Torr H20 chamber pressure.

Phosphorus (POX)only appears on the MSDS of the Edwards oil as a combustion product but, vacuum gurus from Varian think that in order for mechanical pump backstreaming to occur pressures well below (100 millitorr) need to be present for pressure equilibriation and subsequent backstreaming. Even then with a 2 torr H2O pressure I expect the P traces should still be minimal preventing the contaminant from completely overtaking the process.

I would be greatful for an input as I am currently at a loss. I have attempted numerous methods (EDS, EBSD, XRD, XPS, TEM) in trying to get a clue to this problem and am yet to come up with any real conclusions.




---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 20:42:16 2005



From: Palash.Gangopadhyay-at-fys.kuleuven.ac.be (by way of Ask-A-Microscopist)
Date: Tue, 1 Feb 2005 20:40:39 -0600
Subject: [Microscopy] AskAMicroscopist: online AFM / MFM / STM course?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Palash.Gangopadhyay-at-fys.kuleuven.ac.be) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, February 1, 2005 at 12:27:56
---------------------------------------------------------------------------

Email: Palash.Gangopadhyay-at-fys.kuleuven.ac.be
Name: Dr. Palash Gangopadhyay

Organization: Molecular and Nano Materials, K U Leuven

Education: Graduate College

Location: Leuven, Belgium

Question: Dear Microscopists

I will appreciate very much if somebody can direct me to an online AFM / MFM / STM course immediately available.

Thanks in Advance
Palash Gangopadhyay

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 1 20:55:11 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Tue, 01 Feb 2005 18:52:44 -0800
Subject: [Microscopy] Re: embedding tiny specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Diana,
Many years ago we often embedded spermatozoa which were also invisible.
We got around it by staining them in methylene blue which gave them some
color so we could find them in the capsule. As you did, we embedded
them in a minimum of 2% agar which held them together while they were
processed. Don't know if the MB would stain what you are looking at,
but it might be worth a try or even perhaps some stain that might stick
to what is in your sample if MB doesn't work.

Good Luck,

Judy

Judy Murphy, PhD
Microscopy, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net


Diana van Driel wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Hi all,
}
} I've been given some vanishingly small specimens to embed for TEM.
} They're peels from the surface of a retina - at most several cells
} thick and usually less than 1x2 mm in area. They are also made mostly
} of basement membrane, with a few whole cells and some cell fragments,
} so not only are they almost invisible to start with, but they remain
} invisible even after osmium. Pinning them down won't work - they're
} too small. I've embedded some once using a dissecting microscope, but
} it was a lot of work and even so I lost 2 of the 6. I thought of
} embedding first in agar, but then I'll end up with an invisible
} specimen somewhere in the resin, which will take longer find by serial
} sectioning than I'm prepared to spend.
}
} Any ideas would be most welcome!
}
} Diana
}
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney, NSW
} AUSTRALIA 2001
}
} Phone 61 2 93827278
} Mobile 0423 151614
} FAX 61 2 93827318
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 2 08:27:30 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 02 Feb 2005 09:24:50 -0500
Subject: [Microscopy] Re: embedding tiny specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Diana,
Try putting the ideas from Paul Webster and Judy Murphy together...if
you've got mostly basement membrane, then a cationic dye, like alcian
blue or ruthenium red will take quite nicely. You may need to play a
bit with concentrations...both can get a little murky if you use too
much. Years ago, I did some studies that looked at the proteoglycans
in the heart using a variety of cationic dyes, as I recall we used
concentrations that were on the order of 0.05-0.1% dye (wt-vol).
Good luck,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 2 09:07:23 2005



From: Patricia Scallion :      PSCALLIO-at-DAL.CA
Date: Wed, 2 Feb 2005 11:05:16 -0400
Subject: [Microscopy] SEM: using a hot glue gun to fix samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I wanted to know if anyone has much success with the hot glue gun sold by
Cedarlane Labs. They cite a paper in the Proceedings of the 50th Annual Meeting
of the EMSA/MAS/MSC.SMC in 1992, pages 410-411, to describe its merits for fast
fixing of samples for SEM.

Thanks,
Pat
Research Technician
SEM-FIB Facility
Institute for Research in Materials
Dalhousie University
(902) 494-1258


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 2 11:32:09 2005



From: Paul Webster :      pwebster-at-hei.org
Date: Wed, 02 Feb 2005 09:28:14 -0800
Subject: [Microscopy] Re: Re: embedding tiny specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello again,

In my original message I gave a brief overview of how I handle small
specimens. It is obvious that I missed out many details (as pointed out
off-line) so here is a slightly more detailed description of how I do it.

First, the cell fragments/specimen/cells/junk etc is fixed in aldehyde. It
is then pelleted down and washed with buffer containing glycine. The glycine
reacts with the aldehyde and stops it cross-linking the gelatin.

I then mix the pellet with a small amount of 10% gelatin (for those in the
US, Knox gelatin works really well, for the rest of the world, try food
quality gelatin it doesn't precipitate phosphates). The gelatin is warm (37
degrees) and should remain warm while the specimen is mixed with it and
centrifuged down. The pellet can be centrifuged into a small tube with a
pointed base, or placed using a fixed angle rotor. Cooling the gelatin will
make it gel (I know it is obvious but apparently it needed to be stated).

Once the gelatin has set, the pellet is cut out and fixed again in aldehyde.
It can then be treated as a piece of tissue and processed in any way you
like. If it is to be embedded in epoxy resin, then osmium treatment it
recommended. This will turn the gelatin brown and make it easy to see in the
polymerized plastic.

If Lowicryl or LR White embedding is required, then I would suggest using
agarose embedding as an alternative. However, gelatin is liquid at 37
degrees but agarose has to be heated to 60 degrees, which may affect
antigenicity. To make the block visible in the resin I add color to the
agarose or gelatin. I do try to avoid staining the specimen.

An alternative approach to handling really small specimens is to mix them
with something that bulks up the pellet but is easily identified in the
pellet. RBC membranes make a good padding for nucleated cells and there is
no way you can mix one up for another.

You could also mix your small sample with fibrin and polymerize it using
fibrinogen (see Acta Histochem. 1998 Jul;100(3):309-13. Processing of free
cells for electron microscopy using a fibrin clot. Raska I, Pliss A, Mandys
V, Risueno MC, Lojda Z. for details of this). The clot is easy to handle and
bulks up the pellet sufficiently to see it when it is embedded.

Finally, if you pellet the specimen, embed it in gelatin as I described
above, you can take out the pellet and place it onto a glass slide covered
with Parafilm. Pour warm gelatin over the pellet and press a second
Parafilm-coated slide over the first. Use spaces to create a space between
the two slides so that when you take the slides apart after the gelatin has
gelled, you will have your specimens embedded in a thin film of gelatin. You
may be surprised by how easily the specimen can be seen in this thin film.
Cutting out the specimen is very easy, but remember to keep the gelatin cool
or it will become liquid.

Good luck,

Paul Webster.



On 2/2/05 6:24 AM, "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Diana,
} Try putting the ideas from Paul Webster and Judy Murphy together...if
} you've got mostly basement membrane, then a cationic dye, like alcian
} blue or ruthenium red will take quite nicely. You may need to play a
} bit with concentrations...both can get a little murky if you use too
} much. Years ago, I did some studies that looked at the proteoglycans
} in the heart using a variety of cationic dyes, as I recall we used
} concentrations that were on the order of 0.05-0.1% dye (wt-vol).
} Good luck,
} Lee



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 2 14:48:20 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 02 Feb 2005 15:46:01 -0500
Subject: [Microscopy] Re: Re: Re: embedding tiny specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sigma makes low-temperature gelling agarose. Type VII has a gel point of
about 30oC. We use this routinely for all samples...even those for ICC. We
just dissolve the agarose (usually a 1.5% solution) and then cool it to
about 40oC before adding it to samples. This is well below the temperature
that should affect antigenicity but is still high enough to keep the agarose
fluid while you are pelleting. I try to put the tubes in warm water when
possible(depending on centrifuge) while spinning to also slow down the
gelling.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

On 2/2/05 12:28 PM, "Paul Webster" {pwebster-at-hei.org} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Hello again,
}
} In my original message I gave a brief overview of how I handle small
} specimens. It is obvious that I missed out many details (as pointed out
} off-line) so here is a slightly more detailed description of how I do it.
}
} First, the cell fragments/specimen/cells/junk etc is fixed in aldehyde. It
} is then pelleted down and washed with buffer containing glycine. The glycine
} reacts with the aldehyde and stops it cross-linking the gelatin.
}
} I then mix the pellet with a small amount of 10% gelatin (for those in the
} US, Knox gelatin works really well, for the rest of the world, try food
} quality gelatin it doesn't precipitate phosphates). The gelatin is warm (37
} degrees) and should remain warm while the specimen is mixed with it and
} centrifuged down. The pellet can be centrifuged into a small tube with a
} pointed base, or placed using a fixed angle rotor. Cooling the gelatin will
} make it gel (I know it is obvious but apparently it needed to be stated).
}
} Once the gelatin has set, the pellet is cut out and fixed again in aldehyde.
} It can then be treated as a piece of tissue and processed in any way you
} like. If it is to be embedded in epoxy resin, then osmium treatment it
} recommended. This will turn the gelatin brown and make it easy to see in the
} polymerized plastic.
}
} If Lowicryl or LR White embedding is required, then I would suggest using
} agarose embedding as an alternative. However, gelatin is liquid at 37
} degrees but agarose has to be heated to 60 degrees, which may affect
} antigenicity. To make the block visible in the resin I add color to the
} agarose or gelatin. I do try to avoid staining the specimen.
}
} An alternative approach to handling really small specimens is to mix them
} with something that bulks up the pellet but is easily identified in the
} pellet. RBC membranes make a good padding for nucleated cells and there is
} no way you can mix one up for another.
}
} You could also mix your small sample with fibrin and polymerize it using
} fibrinogen (see Acta Histochem. 1998 Jul;100(3):309-13. Processing of free
} cells for electron microscopy using a fibrin clot. Raska I, Pliss A, Mandys
} V, Risueno MC, Lojda Z. for details of this). The clot is easy to handle and
} bulks up the pellet sufficiently to see it when it is embedded.
}
} Finally, if you pellet the specimen, embed it in gelatin as I described
} above, you can take out the pellet and place it onto a glass slide covered
} with Parafilm. Pour warm gelatin over the pellet and press a second
} Parafilm-coated slide over the first. Use spaces to create a space between
} the two slides so that when you take the slides apart after the gelatin has
} gelled, you will have your specimens embedded in a thin film of gelatin. You
} may be surprised by how easily the specimen can be seen in this thin film.
} Cutting out the specimen is very easy, but remember to keep the gelatin cool
} or it will become liquid.
}
} Good luck,
}
} Paul Webster.
}
}
}
} On 2/2/05 6:24 AM, "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} wrote:
}
} }
} }
} }
-----------------------------------------------------------------------------} }
-
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
------------------------------------------------------------------------------}
}
} -
} }
} } Diana,
} } Try putting the ideas from Paul Webster and Judy Murphy together...if
} } you've got mostly basement membrane, then a cationic dye, like alcian
} } blue or ruthenium red will take quite nicely. You may need to play a
} } bit with concentrations...both can get a little murky if you use too
} } much. Years ago, I did some studies that looked at the proteoglycans
} } in the heart using a variety of cationic dyes, as I recall we used
} } concentrations that were on the order of 0.05-0.1% dye (wt-vol).
} } Good luck,
} } Lee
}
}




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 2 15:57:10 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 02 Feb 2005 15:54:23 -0600
Subject: [Microscopy] Re: AskAMicroscopist: online AFM / MFM / STM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Palash,

The new Nanotech America site just launched this morning at www.nanotech-america.com. If you look under "How SPMs work", you will find a animations of all the major imaging and measurement modalities. Also, there is a textbook which can be downloaded under The Library.

Hope this is helpful.

Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
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Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.



At 08:40 PM 2/1/2005, Palash.Gangopadhyay-at-fys.kuleuven.ac.be wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 2 18:07:45 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Wed, 2 Feb 2005 22:32:57 +0100
Subject: [Microscopy] Re: embedding tiny specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Patricia,
I don't know about the hot glue gun sold by Cedarlane, but I have been using
a household-type hot glue gun to fix samples on an SEM stub for twenty
years. (I've been through several guns and learned to get the more expensive
ones.) I find it is good for heavier or odd-shaped samples and gives a more
secure hold with no creep than the sticky tabs, but it is not conductive, so
you have to make a path to ground over the glue after it is hard. I use a
stripe of carbon paint. What is your problem with it?
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "Patricia Scallion" {PSCALLIO-at-DAL.CA}
To: {Microscopy-at-MSA.Microscopy.com}
Sent: Wednesday, February 02, 2005 7:05 AM

(Sorry about all of the } } } , please ignore them. )
} } } Diana,
} } } This sounds like a job for the Formvar sandwich. Here is a
} } } description I wrote as applied to the tiny arabidopsis root tip, but
} } } the problem is the same. If you have further questions, please email.
} } } Note that Formvar stands up just fine to acetone and epoxies but
} } } propylene oxide will dissolve it. Good luck. TB.
} } }
} } }
} } } The small size of arabidopsis roots (ca 0.15 mm diameter) makes them
} } } easy to lose while changing solutions. To retain the roots, I use a
} } } method that is not only convenient but also turns out to be
} } } beneficial for sample preservation. Originally, I encased each root
} } } in a small droplet of low-gelling-temperature agarose, but this is
} } } messy and exposes the sample to heat, albeit briefly. Then, I
} } } modified a method from cryofixation where samples are mounted on a
} } } Formvar film. A chemically fixed root tip is placed on a
} } } Formvar-coated wire loop, a second Formvar film secures the root tip
} } } on the loop. The Formvar films are readily permeated by solvents and
} } } small molecules. Between Formvar films, the thin arabidopsis root tip
} } } is prevented from bending or twisting. I call this "mechanical
} } } fixation" and beyond being convenient, it seems to enhance sample
} } } preservation.
} } } Loops are made in advance and coated by casting Formvar
} } } rectangles (measuring a little more than the loop diameter on one
} } } side and a little more than twice the loop diameter on the other) and
} } } plunging the loop into the water over the rectangle so that the plane
} } } of the loop bisects the long axis of the rectangle. The Formvar
} } } rectangle wraps around the wire loop and the coated loop is removed
} } } at once from the water. Such loops remain stable for months. To
} } } secure a sample, the procedure is repeated: After the sample has been
} } } fixed and rinsed, a loop (already Formvar coated) is placed
} } } horizontally on a drop of water (or buffer) and the sample placed on
} } } the Formvar. Excess sample is trimmed if needed, and the loop (with
} } } sample) is plunged onto a new Formvar rectangle, thus encasing the
} } } sample between Formvar layers, held by the loop. Several loops can be
} } } placed in a vial and solutions exchanged without losing the sample.
} } } The loop is embedded with the sample, and removed during trimming. I
} } } use fine copper wire (36 gauge), which can be trimmed along with the
} } } block.
} } }
} } }
} } } }
} } } } Hi all,
} } } }
} } } } I've been given some vanishingly small specimens to embed for TEM.
} } } } They're peels from the surface of a retina - at most several cells
} } } } thick and usually less than 1x2 mm in area. They are also made
} } } } mostly of basement membrane, with a few whole cells and some cell
} } } } fragments, so not only are they almost invisible to start with, but
} } } } they remain invisible even after osmium. Pinning them down won't
} } } } work - they're too small. I've embedded some once using a dissecting
} } } } microscope, but it was a lot of work and even so I lost 2 of the 6.
} } } } I thought of embedding first in agar, but then I'll end up with an
} } } } invisible specimen somewhere in the resin, which will take longer
} } } } find by serial sectioning than I'm prepared to spend.
} } } }
} } } } Any ideas would be most welcome!
} } } }
} } } } Diana
} } } }
} } } }
} } } }
} } } } Diana van Driel
} } } } Dept Ophthalmology
} } } } Sydney University
} } } } GPO Box 4337
} } } } Sydney, NSW
} } } } AUSTRALIA 2001
} } } }
} } } } Phone 61 2 93827278
} } } } Mobile 0423 151614
} } } } FAX 61 2 93827318
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \
University of Massachusetts
/ / / \ \ \
Amherst, MA, 01003
/ / ___ / \ \__/ \ ____ Voice: 413 - 545 - 1533
Fax: 413 - 545 - 3243
http://www.bio.umass.edu/biology/baskin/
--


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 00:50:33 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 3 Feb 2005 00:49:31 -0600
Subject: [Microscopy] Looking For a Copy of 1994 Microscopy Today Issue#2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Does anyone have in their archives a complete copy of
the 1994 Microscopy Today Magazine Issue#2?

If someone has a copy can you please contact me off
line so that I can arrange to borrow it to digitize/scan it
for the MSA Archives?

It is the only missing issue from the Microscopy Today Archives
which MSA/MT is assembling.

Thanks.

Nestor

Your FriendlyNeighborhood SysOp


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 11:06:22 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 3 Feb 2005 11:05:18 -0600
Subject: [Microscopy] Administrivia: Jan 2005 Archives now on-line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

The Jan 2005 Archives of the Microscopy Listserver are now on-line.

http://www.microscopy.com/MicroscopyListserver


Cheers

Nestor
Your Friendly Neighborhood SysOp



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 11:05:38 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Thu, 03 Feb 2005 11:01:33 -0600
Subject: [Microscopy] Automated in situ and ab staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our campus-wide, multi-user microscopy imaging facility is considering the
purchase of an instrument that would process slides for in situ
hybridization or antibody staining (e.g., Intavis InsituPro VS). I am
skeptical on the usefulness of these type of instruments in a multi-user
environment where the reagents and staining conditions change on a daily
basis. Are there any core facilities out there that are successfully using
one? Do you recover the costs of consumables and make enough to cover the
service contract and staff time? Your public or private responses would be
welcome. Thanks, Tom Phillips

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 12:26:09 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Thu, 3 Feb 2005 10:37:44 -0800
Subject: [Microscopy] Lattice constant of gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,
I am trying to find the best value for the lattice constant of gold at
82 K. I have only found the values for room temp and above. Does
anyone have a reference for this value at low temperature? TIA.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 12:31:25 2005



From: Jan Factor :      jfactor-at-ns.purchase.edu
Date: Thu, 03 Feb 2005 13:30:47 -0500
Subject: [Microscopy] Request about Orion 6 digital system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues: I am considering the Orion 6 system for passive digital
image collection from my old analog SEM. Does anyone have personal
experience (positive or negative) with the Orion system they would care
to share? I would be happy to have your candid comments off-list. Thank you.
--Jan Factor

---------------------------------------2/3/05
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College
State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 13:28:49 2005



From: Richard Harris :      rjharris-at-uwo.ca
Date: Thu, 03 Feb 2005 14:25:19 -0500
Subject: [Microscopy] Re: Embedding woody specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers
Thank you for you many(!) helpful and useful responses. Our researcher is
overwhelmed by your generous help.

Richard Harris
Laboratory Supervisor
Department of Biology
University of Western Ontario
London Ontario CANADA
N6A 5B7
Ph. 519-661-2111 ext. 86780
Fax 519-661-3935



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 13:53:02 2005



From: Johnson, Teri :      TJJ-at-Stowers-Institute.org
Date: Thu, 3 Feb 2005 13:51:19 -0600
Subject: [Microscopy] Automated in situ and ab staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom,

I would be most hesitant to get one of these instruments and put it in a
multi-user environment. We have the InSituPro unit (previous
generation) for whole mount staining. We do all of the staining with
this and charge back what it costs to do the service. We do not charge
for staff time. One of the PIs here also has a unit, and they have one
dedicated operator in their lab. The post-docs will bring their samples
and probes, she will load them and do the runs, and then give them back
to the post-doc for the chromogen step.

I caution against getting the VS for staining on slides as this
application is still undergoing development. Personally I would prefer
to wait until it has finished development and undergone field testing
for a bit before committing $$$s to the instrumentation. I think the
technology has promise and was very much encouraged by what I saw at the
demo.

In my opinion, the best results from equipment such as this require
dedicated operators. Yes, many people can be trained on using the
instrument because it's not rocket science. But too many cooks that
have their hands in the pot (so to speak), is a recipe for disaster. One
day someone will come in and someone previously has toyed with the
settings just enough that their run will be ruined. Or you'll have all
your samples ready to run, only to find out the machine is not working
properly, won't initialize, whatever, and have no indication from the
previous user there was a problem.

But then again, maybe I'm just a control freak. LOL

Best of luck to you,

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
tjj-at-stowers-institute.org




-----Original Message-----
} From: Tom Phillips [mailto:phillipst-at-missouri.edu]
Sent: Thursday, February 03, 2005 11:02 AM
To: Microscopy-at-msa.microscopy.com

Our campus-wide, multi-user microscopy imaging facility is considering
the
purchase of an instrument that would process slides for in situ
hybridization or antibody staining (e.g., Intavis InsituPro VS). I am
skeptical on the usefulness of these type of instruments in a multi-user

environment where the reagents and staining conditions change on a daily

basis. Are there any core facilities out there that are successfully
using
one? Do you recover the costs of consumables and make enough to cover
the
service contract and staff time? Your public or private responses would
be
welcome. Thanks, Tom Phillips

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu






From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 16:06:18 2005



From: Rosemary White :      Rosemary.White-at-csiro.au
Date: Fri, 04 Feb 2005 09:04:32 +1100
Subject: [Microscopy] FITC as a stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

Apologies in advance for double post to confocal and microscopy list.

The group studying flour and dough structure here wants to use FITC to stain
the dough protein so they can get an idea of the macrostructure of the
dough. The protocol has been published in a couple of papers from another
group, and after staining the blob of dough they looked at it under confocal
to see changes in fluorescence pattern after various amounts of mixing, and
assumed that any fluorescence came from protein aggregates. This worries
me, as I wasn't aware that FITC per se was such a specific stain for
protein, and the published protocol says nothing about washing out excess
free dye. The alternative I thought of was to use fluorescamine, which is
only fluorescent when bound to protein, so you could put your sample into
the dye and observe without rinsing. (Needs UV, though.)

Does anyone have experience with this sort of material, comments about FITC
as a protein stain?

TIA,
Rosemray

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 16:13:10 2005



From: Doug Cromey :      dcromey-at-email.arizona.edu
Date: Thu, 03 Feb 2005 15:11:02 -0700
Subject: [Microscopy] Re: RE: Automated in situ and ab staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is the Intavis InsituPro VS similar to the automated immunohistochemistry
and In Situ units sold by Ventana Medical Systems? The Ventana systems
were developed at the University of Arizona and they seem to work well
if you are doing the same sort of staining runs on histologic samples
(paraffin or frozen sections) over and over again. I gather that there's a
bit of a learning curve to know how to program in a new procedure.

Doug

Caveat: I have no financial interest in VMS, but I do know the founders
and have several friends who work for the company.
....................................................................
Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy
Research Specialist, Principal University of Arizona
(office: AHSC 4212A) P.O. Box 245044
(voice: 520-626-2824) Tucson, AZ 85724-5044 USA
(FAX: 520-626-2097) (email: Cromey-at-Arizona.edu)
....................................................................
http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 17:07:56 2005



From: Connie McManus :      conniemoss-at-relia.net
Date: Thu, 3 Feb 2005 16:06:09 -0700 (MST)
Subject: [Microscopy] Re: automated in situ and ab staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dr. Phillips,
I have 30 years of experience in histology and close to 6 years of
experience doing antibody staining. I don't know what the environment of
your lab is, but in any lab where someone is doing a LOT of IHC (antibody
staining) or ish (in situ hybridization) staining, automation is the way
to go. The technology of these stainers has become really good and many of
the bugs in the older instruments that you may be thinking of have pretty
much been worked out.
I'm not familiar with the stainer you mentioned, but I am familiar with
Ventana Medical Systems and DAKOCytomation. IMO, DAKO makes one of the
best and also happens to be the least costly because you can use whatever
reagents you choose. Ventana makes a fabulous machine, but the one I used
in my former job was tied in to the use of their reagents only. Their
detection kits run over 2000USD per kit and each kit performs only 250
tests. I think Ventana now makes another stainer that is more reagent
flexible. THey're a great company, just really expensive. I cannot advise
you on whether it is economical for your lab to invest in this as I don't
know how much work you're doing and what you charge per slide vs your
overhead in salaries, etc. You can figure that out easily enough.

If there are to be mutliple users of these instruments, that is usually no
problem as the manufacterer usually provides a training session for all
employees. When you get your stainer, I would mandate that all techs who
will use the instrument must be trained first. In most facilities,
training is done not only when a new instrument arrives, but also a
periodic check with each tech to ensure they are doing things right.

I hope this is helpful

Connie McManus
Mt Ogden Scientific Services
950 W Kershaw, Suite E
Ogden UT 84401
tel: 866/933-6677
conniemoss-at-relia.net
www.mtogdensci.com (not yet available)



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 20:28:41 2005



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 4 Feb 2005 13:26:33 +1100
Subject: [Microscopy] Embedding tiny specimen - lost Email and thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to everyone. I now have a collection of ideas to try! Could the
person who suggested blue material and who had the PowerPoint
presentation please resend me the Email and I would love the
presentation as well; somehow I managed to delete your message.

Diana


Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 22:56:54 2005



From: anith nelleri :      anith_n-at-yahoo.com
Date: Thu, 3 Feb 2005 20:55:14 -0800 (PST)
Subject: [Microscopy] regarding simulated specimens for microscopy experiments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

I'm a graduate student from India. I'm working on
digital holographic imaging. I am intereted to extend
it for microscopic imaging . Recently I came to know
that, there are simulated specimens (both amplitude
and phase objects- phantoms-synthetic) available for
microscopy experiments. Are there any suppliers where
I can place orders for such objects according to my
requirements or may be for the readily available
samples.

Hoping your replies
Sincerely,
Anith.N




__________________________________
Do you Yahoo!?
All your favorites on one personal page – Try My Yahoo!
http://my.yahoo.com


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 3 23:56:27 2005



From: George Theodossiou :      George.Theodossiou-at-amcor.com.au
Date: Fri, 4 Feb 2005 16:55:17 +1100
Subject: [Microscopy] ISIS Spectrum Export

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

Last year I posed a question on whether anyone had a method or software that
could read and batch export ISIS maps and images from a job rather than
doing it individually. I got great support from several individuals and now
have a some great pieces of software that I use regularly.
As a follow on I now need to do the same with the ISIS spectra. Is there
anyone out there who has a method or software for reading the ISIS spectra
and batch saving the files as txt, tif or jpg.

Any help would be greatly appreciated.

Regards
George


George Theodossiou
Physicist / Microscopist
Microscopy and Microanalysis Laboratory

AMCOR Research and Technology
Ph: +61 3 9490 6135
Fax: +61 3 9499 4295
Mobile: 0409 568 840
email: George.Theodossiou-at-amcor.com.au


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please notify AMCOR immediately.
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From MicroscopyL-request-at-ns.microscopy.com Fri Feb 4 07:23:13 2005



From: Mr Brian Kirkmeyer :      kirk3377-at-yahoo.com
Date: Fri, 4 Feb 2005 05:21:33 -0800 (PST)
Subject: [Microscopy] LM Staining reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I come from an EM background and have recently assumed
responsibility for LM evaluations. However, I am in
need of a reference detailing what stains what, and
for which types of microscopy. Our current microscope
is equipped for UV/fluorescence (among other things),
and this is of primary interest to me at this point.

So if anyone can point me toward an LM staining
reference, I would greatly appreciate it. Thanks!

Kirk

Brian (Kirk) Kirkmeyer, Ph.D.
Research Scientist, Microscopy
International Flavors and Fragrances
1515 State Highway 36
Union Beach, NJ 07735-3542
732-335-2426 / 732-335-2350 FAX
brian.kirkmeyer-at-iff.com



__________________________________
Do you Yahoo!?
Yahoo! Mail - 250MB free storage. Do more. Manage less.
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From MicroscopyL-request-at-ns.microscopy.com Fri Feb 4 08:26:06 2005



From: Richard Fiore :      rfiore-at-juno.com
Date: Fri, 4 Feb 2005 09:22:49 -0500
Subject: [Microscopy] Re: AskAMicroscopist: online AFM / MFM / STM course?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Though not an on-line course, you should consider the AFM short course
(one week) at NC State University. Check it out at
http://www.ncsu.edu/aif/afmcourse

Lehigh U also has a course but they cancelled it last year so I am not
sure if it will be held this year or not.

On Tue, 1 Feb 2005 20:40:39 -0600 Palash.Gangopadhyay-at-fys.kuleuven.ac.be
(by way of Ask-A-Microscopist) writes:
}
}
}
-------------------------------------------------------------------------
-----
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------
------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (Palash.Gangopadhyay-at-fys.kuleuven.ac.be) from
} http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on
} Tuesday, February 1, 2005 at 12:27:56
}
-------------------------------------------------------------------------
--
}
} Email: Palash.Gangopadhyay-at-fys.kuleuven.ac.be
} Name: Dr. Palash Gangopadhyay
}
} Organization: Molecular and Nano Materials, K U Leuven
}
} Education: Graduate College
}
} Location: Leuven, Belgium
}
} Question: Dear Microscopists
}
} I will appreciate very much if somebody can direct me to an online
} AFM / MFM / STM course immediately available.
}
} Thanks in Advance
} Palash Gangopadhyay
}
}
-------------------------------------------------------------------------
--
}
}
}


Richard Fiore, Southeast Sales
Carl Zeiss SMT (formerly LEO Electron Microscopy)
5127 Orange Grove Rd., Hillsborough, NC 27278
Tel: 919-643-2234, Cell: 919-593-1960, Fax: 919-643-2667
Email: rfiore-at-juno.com or fiore-at-smt.zeiss.com, URL:
www.smt.zeiss.com/nts


From MicroscopyL-request-at-ns.microscopy.com Fri Feb 4 08:43:34 2005



From: Johnson, Teri :      TJJ-at-Stowers-Institute.org
Date: Fri, 4 Feb 2005 08:41:52 -0600
Subject: [Microscopy] Re: automated in situ and ab staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ditto what Connie said about the Dako instrument...it seems to be the
"industry standard" for automated IHC. However, it does not have
capability for doing in situ hybridization. We currently have the
Ventana Discovery to do the automated ISH on slides, in addition to the
InSituPro for doing automated ISH on whole mounts. What makes the new
InSituPro VS unit so compelling is that you can do either whole mounts
or slide-mounted tissues on it on any given run.

To further clarify my position on multiple users, in our lab all
technicians are trained on every instrument. My initial response to Dr.
Phillips about having dedicated users was with regard to not making it
available for post-docs and other researchers to just come in and run
the thing, which is a common practice in core facilities.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
tjj-at-stowers-institute.org




-----Original Message-----
} From: Connie McManus [mailto:conniemoss-at-relia.net]
Sent: Thursday, February 03, 2005 5:06 PM
To: microscopy-at-msa.microscopy.com

Dr. Phillips,
I have 30 years of experience in histology and close to 6 years of
experience doing antibody staining. I don't know what the environment of
your lab is, but in any lab where someone is doing a LOT of IHC
(antibody
staining) or ish (in situ hybridization) staining, automation is the way
to go. The technology of these stainers has become really good and many
of the bugs in the older instruments that you may be thinking of have
pretty much been worked out. I'm not familiar with the stainer you
mentioned, but I am familiar with
Ventana Medical Systems and DAKOCytomation. IMO, DAKO makes one of the
best and also happens to be the least costly because you can use
whatever reagents you choose. Ventana makes a fabulous machine, but the
one I used in my former job was tied in to the use of their reagents
only. Their detection kits run over 2000USD per kit and each kit
performs only 250 tests. I think Ventana now makes another stainer that
is more reagent flexible. THey're a great company, just really
expensive. I cannot advise you on whether it is economical for your lab
to invest in this as I don't know how much work you're doing and what
you charge per slide vs your overhead in salaries, etc. You can figure
that out easily enough.

If there are to be mutliple users of these instruments, that is usually
no problem as the manufacterer usually provides a training session for
all employees. When you get your stainer, I would mandate that all
techs who will use the instrument must be trained first. In most
facilities, training is done not only when a new instrument arrives, but
also a periodic check with each tech to ensure they are doing things
right.

I hope this is helpful

Connie McManus
Mt Ogden Scientific Services
950 W Kershaw, Suite E
Ogden UT 84401
tel: 866/933-6677
conniemoss-at-relia.net
www.mtogdensci.com (not yet available)





From MicroscopyL-request-at-ns.microscopy.com Fri Feb 4 10:57:23 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Fri, 04 Feb 2005 10:54:44 -0600
Subject: [Microscopy] Re: LM Staining reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Kirk,

If fluorescence is your primary interest I would strongly recommend that you visit websites for the following companies:
Molecular Probes - they make the broadest range of fluorescence probes and have very rich content as well as gorgeoous images
Quantum Dots - a new approach to fluorescent staining. Instead of being based on organic molecules, the qDots are derived from more inert, semiconductor based materials.

The two primary filter manufacturers in our industry are Omega Filters (www.omegafilters.com) and Chroma. Both have, again, very informative websites.

Re: learning more about fluorescence - I would encourage you to visit the Nikon mini-university, Olympus' site, and (at the risk of being a bit self-serving), getting a copy of Optimizing Light Microscopy (visit www.microscopyeducation for details).

Hope this was helpful
Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.

Caveat: MME has no financial involvement in any of the companies mentioned above.


At 07:21 AM 2/4/2005, Mr Brian Kirkmeyer wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Fri Feb 4 11:42:56 2005



From: Connie McManus :      conniemoss-at-relia.net
Date: Fri, 4 Feb 2005 10:41:00 -0700 (MST)
Subject: [Microscopy] Re: LM staining reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kirk,

I assume you want to stain something with a fluorescent antibody of some
kind. This is not in my area of expertise, but from what I understand,
before you do that, you need to have some kind of stained section to get a
fix on where you are in the tissue. Also, it is important to know what
kind of tissue prep ... FFPE (formalin fixed paraffin embedded) or are
the tissues processed in plastic? Are these sectioned at 3-5 um? more or
less that that? Do you want special stains ((i.e. stains for connective
tissues, glycogen, fungi, bacteria, mast cells ... ad infinitum) or do you
just want a plain, simple stain that will show the basic cell arrangements
in the tissue ???? What do you want to see? There are some excellent
texts out there. Perhaps the best one for starters is Frieda Carson's
book on Histotechnology. Can be purchased from Sigma, Amazon.com, Barns
Noble, etc. and it's fairly inexpensive. There are tons and tons of
really good histology books out there. Also, it doesn't hurt to have a
sit down chat with your technicians and find out from them what they can
do and what they would recommend.

I'm sorry not to be of more help. Please feel free to contact me
privately if you need help of any kind.

Connie McManus
Mt Ogden Scientific Services
950 W Kershaw, Suite E
tel: 866/933-6677
fax: 435/514-1781
conniemoss-at-relia.net
www.mtogdensci.com


------------------------------------------
} } Kirk wrote: { {
I come from an EM background and have recently assumed
responsibility for LM evaluations. However, I am in
need of a reference detailing what stains what, and
for which types of microscopy. Our current microscope
is equipped for UV/fluorescence (among other things),
and this is of primary interest to me at this point.

So if anyone can point me toward an LM staining
reference, I would greatly appreciate it. Thanks!

Kirk




From MicroscopyL-request-at-ns.microscopy.com Fri Feb 4 16:35:09 2005



From: David Vowles :      djv23-at-msm.cam.ac.uk
Date: Fri, 4 Feb 2005 22:33:07 +0000 (GMT)
Subject: [Microscopy] Re: ISIS Spectrum Export

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


George Theodossiou {George.Theodossiou-at-amcor.com.au} wrote:
}
} ...Is there anyone out there who has a method or software for reading
} the ISIS spectra and batch saving the files as txt, tif or jpg.
}
I have written some software that will convert ISIS spectra to JPEG, BMP
or text format. You can convert files singly or as a batch. The programme
also reads, displays and converts other Link/Oxford formats, as well as
Noran, Edax and EMSA formats.If you can give me your mailing address I
will send you a copy.

David Vowles
Electron Microscope Unit
Dept of Materials Science and Metallurgy
University of Cambridge
Pembroke St Cambridge
UK CB2 3QZ
Tel: +44 (0)1223 334325
Fax: +44 (0)1223 334567
Email: djv23-at-cam.ac.uk



From MicroscopyL-request-at-ns.microscopy.com Fri Feb 4 17:54:10 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Fri, 04 Feb 2005 17:51:51 -0600
Subject: [Microscopy] Re: Re: LM Staining reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Several of you have written re: problems geting to www.microscopyeducation.com. Much to my chagrin, the links mentioned in my earlier posting got crossed with those of our sister group. I've spoken to the webmaster and he will have it fixed over the weekend. Please try again. ... Follow the Nav buttons in The Library to link to Optimizing Light Microscopy.

Thanks :-)
Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.


At 10:54 AM 2/4/2005, Barbara Foster wrote:




} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Fri Feb 4 19:20:51 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 04 Feb 2005 17:19:14 -0800
Subject: [Microscopy] Zeiss/LEO Supra topics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Listers:

Is there a separate formal list for Zeiss/LEO
Supra users? If so, I'd appreciate knowing
about it. If not, I would like to communicate
with others about discoveries, disappointments, observations,
history, experiences, lessons learned (easy and
hard way) and other topics relative thereto.

We can keep this off of the MSA list and I would
forward all relevant material to those who are
interested.

This is for Supra and SupraVP.

gary g.



From MicroscopyL-request-at-ns.microscopy.com Sat Feb 5 06:29:34 2005



From: Alberto Diaspro :      diaspro-at-fisica.unige.it
Date: Sat, 5 Feb 2005 13:27:33 +0100
Subject: [Microscopy] fib

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear friends,
I am looking for infos for setting up a FIB, dual scan, system.
All my best
AD



------------------------------------------------------------------------
------------------------------------------------------------------------
--------------
[...] Dance with wolves and count the stars, including the
unseen...(L.Ferlinghetti, Challenges to young poet, 2001)

------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------------
Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department
of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309; URL:
http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html

------------------------------------------------------------------------
------------------------------------------------------------------------
------------------------------------------------------------



------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------------
[...] Dance with wolves and count the stars, including the
unseen...(L.Ferlinghetti, Challenges to young poet, 2001)

------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------------
Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department
of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309; URL:
http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html

------------------------------------------------------------------------
------------------------------------------------------------------------
------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Sat Feb 5 08:52:12 2005



From: Valery Ray :      vray-at-partbeamsystech.com
Date: Sat, 5 Feb 2005 06:49:47 -0800 (PST)
Subject: [Microscopy] Re: fib

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Alberto,

What information exactly are you looking for? If you
have specific questions I may be able to help you out.

Disclaimer: PBS&T provides support for charged
particle beam instruments commercially.

Cheers,
=================
Valery Ray
www.partbeamsystech.com

--- Alberto Diaspro {diaspro-at-fisica.unige.it} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Dear friends,
} I am looking for infos for setting up a FIB, dual
} scan, system.
} All my best
} AD
}
}
}
}
------------------------------------------------------------------------
}
}
------------------------------------------------------------------------
}
} --------------
} [...] Dance with wolves and count the stars,
} including the
} unseen...(L.Ferlinghetti, Challenges to young poet,
} 2001)
}
}
------------------------------------------------------------------------
}
}
------------------------------------------------------------------------
}
}
-----------------------------------------------------------
} Alberto Diaspro, MicroScoBIO Research Center,
} LAMBS-IFOM, Department
} of Physics, University of Genoa, Via Dodecaneso 33,
} 16146 Genova, Italy
} facsimile +39-010314218 - voice
} +39-0103536426/480/309; URL:
} http://www.lambs.it
}
}
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html
}
}
------------------------------------------------------------------------
}
}
------------------------------------------------------------------------
}
}
------------------------------------------------------------
}
}
}
}
------------------------------------------------------------------------
}
}
------------------------------------------------------------------------
}
}
-----------------------------------------------------------
} [...] Dance with wolves and count the stars,
} including the
} unseen...(L.Ferlinghetti, Challenges to young poet,
} 2001)
}
}
------------------------------------------------------------------------
}
}
------------------------------------------------------------------------
}
}
-----------------------------------------------------------
} Alberto Diaspro, MicroScoBIO Research Center,
} LAMBS-IFOM, Department
} of Physics, University of Genoa, Via Dodecaneso 33,
} 16146 Genova, Italy
} facsimile +39-010314218 - voice
} +39-0103536426/480/309; URL:
} http://www.lambs.it
}
}
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html
}
}
------------------------------------------------------------------------
}
}
------------------------------------------------------------------------
}
}
------------------------------------------------------------
}
}
}


=====
Valery Ray

Particle Beam Systems
& Technology
www.partbeamsystech.com


From MicroscopyL-request-at-ns.microscopy.com Sat Feb 5 11:25:30 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 05 Feb 2005 09:23:52 -0800
Subject: [Microscopy] Re: Zeiss/LEO Supra topics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As was pointed out to me, the Gemini column
is also on the LEO 1550. So these would apply
as well. I suppose the only main differentiating
factor is plinth and uni-plinth. But the column
seems to be the same.

gary g.


At 05:19 PM 2/4/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Sat Feb 5 19:16:24 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 05 Feb 2005 17:14:41 -0800
Subject: [Microscopy] [MICROSCOPY] Osmium coating resistance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Listers:

Has anyone done any measurements on the resistivity
of Os coatings? Sheet rho vs. thickness, etc.?

If a charging specimen was imaged at 20-25KV, what
Os coating thickness would eliminate the charge?
Then, given that thickness, what would the inherent
sheet resistance be?

gary g.



From MicroscopyL-request-at-ns.microscopy.com Sun Feb 6 08:45:14 2005



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Sun, 6 Feb 2005 15:42:32 +0100
Subject: [Microscopy] Calibration of objectives: thanks!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To all of you who informed me about your experiences, tips & tricks etc.
concerning calibration of objectives, thanks a lot for the massive
input! This states again that this listserver is not just 'a bunch of
people', but a nice collection of pro's with some pro's-to-become, just
as myself (hopefully), who can learn a lot from them!

I calibrated every objective individually with a stage micrometer, but
still noticed about + or - 4% deviation between the 2,5x and 40x
objective. Thanks to your input I now know that it is not so bad, and,
I recalibrated the objectives, but more accurate, and now have about
2,1% difference. Not too bad I think, though it took some time!

Thanks again for your answers, you were of great help!

Sven Terclavers

"A microscopist observes things
which nature hides for the majority,
always feel honoured for that!"



From MicroscopyL-request-at-ns.microscopy.com Sun Feb 6 14:52:40 2005



From: Bobby Hooghan :      hooghan-at-grandecom.net
Date: Sun, 6 Feb 2005 14:49:59 -0600
Subject: [Microscopy] fib

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Alberto,
What is it that you are looking for exactly? Is it for setting up different
scans etc for an existing FIB ? or something else. You can reply on line or
offline,
Best regards,
Bobby Hooghan
Hooghan-at-grandecom.net

-----Original Message-----
} From: Alberto Diaspro [mailto:diaspro-at-fisica.unige.it]
Sent: Saturday, February 05, 2005 6:28 AM
To: MSA listserver

Dear friends,
I am looking for infos for setting up a FIB, dual scan, system.
All my best
AD



------------------------------------------------------------------------
------------------------------------------------------------------------
--------------
[...] Dance with wolves and count the stars, including the
unseen...(L.Ferlinghetti, Challenges to young poet, 2001)

------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------------
Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department
of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309; URL:
http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html

------------------------------------------------------------------------
------------------------------------------------------------------------
------------------------------------------------------------



------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------------
[...] Dance with wolves and count the stars, including the
unseen...(L.Ferlinghetti, Challenges to young poet, 2001)

------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------------
Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department
of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309; URL:
http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html

------------------------------------------------------------------------
------------------------------------------------------------------------
------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Sun Feb 6 19:36:18 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 06 Feb 2005 17:34:35 -0800
Subject: [Microscopy] [MICROSCOPY] Zeiss/LEO Gemini user group

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Listers:

There seems to be some interest in a focused group
for Zeiss/LEO Gemini systems. I have formed a
user group on Yahoo that is open to all Gemini
column system users (if you don't have a Gemini
column system, you likely won't be interested in
this topic/group).

The Gemini systems include all LEO 1500 and Supra
models which are high vacuum and/or variable pressure
(VP). The purpose of the group is to exchange
experiences and info on these systems. Contact
info, service experience, system nuances, macro
editing and creation, etc. are key topics. The group
is not moderated. I started the group but I do not
moderate or edit it. All that is required is vetting--
you need to apply for group membership and then you
are in.

Group Settings:
-listed in directory (does not work at this time--no idea why not)
-membership requires approval
-messages do not require approval
-all members may post messages
-message archive viewable by members only
-e-mail attachments are not permitted

Hopefully this is useful for Gemini users. As a
newbee, I hope to gain from others' experiences.
Perhaps, others can gain from my experiences. A
goal is to not clutter up the MSA list with system-specific
threads. But of course, you can post this topic
if you so desire. General EM topics still should go
to the MSA list. The gemini list should be specific
to this Gemini group of systems (no EVSEM, etc.).

Subscribe: geminisem-subscribe-at-yahoogroups.com
Post: geminisem-at-yahoogroups.com
Unsubscribe: geminisem-unsubscribe-at-yahoogroups.com

If the group does not pan out, I'll cancel it or
turn it over to someone else if there is a desire
to take it on.

gary g.



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 07:42:43 2005



From: Mr Brian Kirkmeyer :      kirk3377-at-yahoo.com
Date: Mon, 7 Feb 2005 05:41:05 -0800 (PST)
Subject: [Microscopy] Re: Re: LM staining reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} From the responses I have received so far, it seems
that I should clarify what I'm looking for a little
bit more. I seek a reference that describes
staining/dyeing/tagging methods for non-histological
samples, as I work with flavor and fragrance chemical
compounds. So recipes for tissue stains or tags,
unless related to a specific chemistry, unfortunately
don't do me a lot of good. If anyone knows of such a
non-histological reference, I would love to hear of
it.

While on the subject, I am also interested in knowing
what stains/dyes/tags are safe for contact with human
skin. Thanks!

Kirk

Brian (Kirk) Kirkmeyer, Ph.D.
Research Scientist, Microscopy
International Flavors and Fragrances
1515 State Highway 36
Union Beach, NJ 07735-3542
732-335-2426 / 732-335-2350 FAX
brian.kirkmeyer-at-iff.com

--- Connie McManus {conniemoss-at-relia.net} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Kirk,
}
} I assume you want to stain something with a
} fluorescent antibody of some
} kind. This is not in my area of expertise, but from
} what I understand,
} before you do that, you need to have some kind of
} stained section to get a
} fix on where you are in the tissue. Also, it is
} important to know what
} kind of tissue prep ... FFPE (formalin fixed
} paraffin embedded) or are
} the tissues processed in plastic? Are these
} sectioned at 3-5 um? more or
} less that that? Do you want special stains ((i.e.
} stains for connective
} tissues, glycogen, fungi, bacteria, mast cells ...
} ad infinitum) or do you
} just want a plain, simple stain that will show the
} basic cell arrangements
} in the tissue ???? What do you want to see? There
} are some excellent
} texts out there. Perhaps the best one for starters
} is Frieda Carson's
} book on Histotechnology. Can be purchased from
} Sigma, Amazon.com, Barns
} Noble, etc. and it's fairly inexpensive. There are
} tons and tons of
} really good histology books out there. Also, it
} doesn't hurt to have a
} sit down chat with your technicians and find out
} from them what they can
} do and what they would recommend.
}
} I'm sorry not to be of more help. Please feel free
} to contact me
} privately if you need help of any kind.
}
} Connie McManus
} Mt Ogden Scientific Services
} 950 W Kershaw, Suite E
} tel: 866/933-6677
} fax: 435/514-1781
} conniemoss-at-relia.net
} www.mtogdensci.com
}
}
} ------------------------------------------
} } } Kirk wrote: { {
} I come from an EM background and have recently
} assumed
} responsibility for LM evaluations. However, I am in
} need of a reference detailing what stains what, and
} for which types of microscopy. Our current
} microscope
} is equipped for UV/fluorescence (among other
} things),
} and this is of primary interest to me at this point.
}
} So if anyone can point me toward an LM staining
} reference, I would greatly appreciate it. Thanks!
}
} Kirk
}
}
}
}





__________________________________
Do you Yahoo!?
Yahoo! Mail - You care about security. So do we.
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From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 09:01:57 2005



From: spradhan-at-siu.edu (by way of MicroscopyListserver)
Date: Mon, 7 Feb 2005 09:00:12 -0600
Subject: [Microscopy] viaWWW: Hitachi S2460 N stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (spradhan-at-siu.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Sunday, February 6, 2005 at 21:39:34
---------------------------------------------------------------------------

Email: spradhan-at-siu.edu
Name: Sailesh Pradhan

Organization: Southern Illinois University, Cabondale

Title-Subject: [Microscopy] [Filtered] MListserver:Hitachi S2460 N stage

Question: Hello list!
I have been looking around, pretty much in vain, for a spare door and stage assembly for a Hitachi S2460N SEM for quite sometime now...We wanted to have it so that we could modify it to suit our purpose for conducting some experiments at our facility here...However, the situation looks very grim to me since i have almost exhausted all possible resources i have know of...could you suggest some more places i could look for it? Or better still, do you think it would be possible to modify such assemblies of other hitachi models to fit our machine?
i shall look forward to your suggestions and advice..thanks a lot in advance...
regards,
sailesh.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 09:02:16 2005



From: cvierret-at-umr.edu (by way of MicroscopyListserver)
Date: Mon, 7 Feb 2005 09:00:47 -0600
Subject: [Microscopy] viaWWW: Ge single crystal for TEM examinatio

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cvierret-at-umr.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, February 7, 2005 at 08:43:52
---------------------------------------------------------------------------

Email: cvierret-at-umr.edu
Name: Clarissa

Organization: University of Missouri/Rolla

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello,

I am looking for information on sample preparation of a Ge single crystal for TEM examination. I need to know how to cut the sample, thin the sample and any other information that would make this job simpler or at least less frustrating.

Thanks for your help in advance.

Clarissa Wisner
UMR-AMCL
Rolla, MO

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 09:12:45 2005



From: medunn-at-mail.uri.edu (by way of MicroscopyListserver)
Date: Mon, 7 Feb 2005 09:11:14 -0600
Subject: [Microscopy] [Filtered] AskAMicroscopist: how to quanitfy the number of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (medunn-at-mail.uri.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, February 7, 2005 at 08:39:20
---------------------------------------------------------------------------

Email: medunn-at-mail.uri.edu
Name: Michael Dunn

Organization: Uinversity of Rhode Island

Education: Graduate College

Location: Kingston, RI

Question: 1)Is there a way to quanitfy the number of cells contained in a tissue sample that is being analyzed on the electron microscope through the use of a software program like NIH?

2)What is the correlation, if any, with the mitochondrial density of a section and the cell count of that same section?

Thanks.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 09:56:09 2005



From: Jan Factor :      jfactor-at-ns.purchase.edu
Date: Mon, 07 Feb 2005 10:55:52 -0500
Subject: [Microscopy] To Tom Phillips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom: Please re-send the email you sent to me over the weekend. (Between
home and work, it ended up on the wrong computer. - Sorry to use the
list for this.)
--Thanks, Jan

---------------------------------------2/7/05
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College, State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 10:57:01 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Mon, 07 Feb 2005 10:54:21 -0600
Subject: [Microscopy] Re: [MICROSCOPY] Osmium coating resistance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Gary,

I'd suggest that you contact Chuck Garber at SPI (www.2SPI.com). He is a wealth of info in this area.

Good hunting!
Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.

Caveat: MME has no financial interest in this product.


At 07:14 PM 2/5/2005, Gary Gaugler wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 11:03:35 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Mon, 07 Feb 2005 11:00:44 -0600
Subject: [Microscopy] Re: Re: Re: LM staining reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, again, Brian

Again, I would really recommend that you investigate fluorescence. The
chemistry is very specific.

As for safety, again, the probe companies have all of that information.

Good hunting,
B

At 07:41 AM 2/7/2005, Mr Brian Kirkmeyer wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 14:45:37 2005



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 7 Feb 2005 15:43:21 -0500
Subject: [Microscopy] viaWWW: Ge single crystal for TEM examinatio

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am not speaking from direct experience for doing Ge, but offhand, Ge should not be more difficult than silicon. If it is a single crystal of Ge, you should know the crystallographic directions of the sample. Of the following, the two least expensive and easy methods would be chemical polishing and small angle cleavage technique.

I would look at several standard techniques to start.

1) if you are doing plan view samples, chemical polishing should work nicely. I don't know the chemical polish for Ge, but I am sure that it is in the literature. Peter Goodhew has a simple chemical polisher apparatus in his book, Thin Foil Preparation for Electron Microscopy (Practical Methods in Electron Microscopy, Vol 11). Basically, it is a tilted rotating cup with a platform in the center where the chemical drips from a burret until the material is perforated. South Bay Technology sells a chemical polisher that can be set up specifically for accurate and reproducible termination, especially if Bernie Kestel's instructions are followed.

2) mechanical polishing, dimpling, and ion milling should work nicely. See the equipment brochures from South Bay Technology, E. A. Fischione, Baltec, and Gatan

3) Ge cleaves. For cross sections and bulk crystal the small angle cleavage technique (microcleave technique) would work very well on this. This gives the best samples known to man (and women). I gave a little short course at UMR a few years ago on SACT. Talk to Lou Ross about it and see South Bay Technology's web site on microcleave for information on this.

4) Tripod Polishing -again see South Bay Technology web site.

5) FIB -Pay some money and have no trouble at all.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)


-----Original Message-----
} From: by way of MicroscopyListserver [mailto:cvierret-at-umr.edu]
Sent: Monday, February 07, 2005 10:01 AM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cvierret-at-umr.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, February 7, 2005 at 08:43:52
---------------------------------------------------------------------------

Email: cvierret-at-umr.edu
Name: Clarissa

Organization: University of Missouri/Rolla

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello,

I am looking for information on sample preparation of a Ge single crystal for TEM examination. I need to know how to cut the sample, thin the sample and any other information that would make this job simpler or at least less frustrating.

Thanks for your help in advance.

Clarissa Wisner
UMR-AMCL
Rolla, MO

---------------------------------------------------------------------------




From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 16:03:06 2005



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 7 Feb 2005 17:00:50 -0500
Subject: [Microscopy] [MICROSCOPY] Osmium coating resistance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,
Just want to clarify something that others might be confused about.

The inherent property to the material that is deposited is its resistivity, not sheet resistance. Sheet resistance is measured usually with a four point probe and has units of ohms per square. It is dependent on the thickness of the material. If you have a square of any size, it will have the same resistance. (It is amazing to see a large 4 foot by 4 foot piece of glass with a conductive coating on it measure the same resistance as a one inch by one inch piece.) If you multiply the sheet resistance by the thickness, you get the resistivity with units ohm-cm.

This all assumes that the layer is uniform across the surface. For very light layers, that may not be the case. For charge drainage, it also assumes that the charge can come to the surface and be drained off. If the conductivity of the sample material is so low that this can't happen, then the relatively high voltage that you are using would put charge deep into the sample. There your charging affects would be voltage dependent. There is an article on that in the latest Microscopy Today issue, isn't there?

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)


-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Saturday, February 05, 2005 8:15 PM
To: MSA listserver

Greetings Listers:

Has anyone done any measurements on the resistivity
of Os coatings? Sheet rho vs. thickness, etc.?

If a charging specimen was imaged at 20-25KV, what
Os coating thickness would eliminate the charge?
Then, given that thickness, what would the inherent
sheet resistance be?

gary g.





From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 16:06:02 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 08 Feb 2005 11:04:15 +1300
Subject: [Microscopy] Coolpix 5700, Lumenera Infinity 3

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Anybody got any experience to share, either good or bad, with the
Lumenera Infinity 3?

Also, does anyone know a source of microscope adaptors for the Nikon
Coolpix 5700?
Presumably they involve also the Nikon UR-E8 adaptor.

cheers and tia

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599
ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 16:07:30 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 07 Feb 2005 17:05:20 -0500
Subject: [Microscopy] processing loose balls of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,
I have just received a request to process balls of what had been
mouse stem cells which have now been differentiated into
cardiomyocytes. They are loose, spongy balls of cells that fall
apart when prodded. The PI would like them embedded in paraffin and
sectioned for light microscopy. If these were for EM, I'd be
perfectly comfortable with the whole thing, but I am a bit boggled
about how to handle infiltration and embedding in wax...they are
essentially transparent and will disappear.
I know that there was a thread about a similar topic a short time
ago, where is was suggested that the samples in question be stained
before embedding. The problem here is that the PI would like to be
able to do a variety of staining protocols on the resultant slides.
Ideas?
thanks,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 7 23:40:14 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Mon, 7 Feb 2005 23:39:12 -0600
Subject: [Microscopy] MM2005 Abstract Deadline Feb 15

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues...

The deadline for receipt of manuscripts for Microscopy & Microanalysis 2005
is 17:00 PST Feb 15, 2005.

Information concerning the program, registration, travel, student
and professional
staff awards, as well as electronic submission is available at
the meeting WWW site.

http://mm2005.microscopy.org

There are a number of new entries for student awards as well as
opportunities
for student discount lodging. These new opportunites have been
established since the original
call for papers the information for which is only available on the
meeting WWW site.

Regards

Nestor
Your Friendly Neighborhood SysOp



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 00:53:08 2005



From: George Theodossiou :      George.Theodossiou-at-amcor.com.au
Date: Tue, 8 Feb 2005 17:51:47 +1100
Subject: [Microscopy] Imaging Starch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

A very broad question to draw out as many thoughts and ideas as possible.
We are at it again in the box factory trying to image starch in paper and
board and I am seeking every scrap of information I can lay my hands on. It
seems we visit this issue every year because we can never seem to find a
satisfactory solution. There are several things we would like to achieve:

1. To image the starch optically and look at its distribution
2. To detect the starch spectroscopically
3. To find a way of differentiating between various starches (wheat, tapioca
and potato)
4. Quantify the starch

We have tried the following in house and using external service providers:
Iodine staining (but it also stains the cellulose, therefore differentiating
starch and fibre is difficult)

Fluorescence microscopy using the stains Calcofluor White (cellulose) and
Con A-FTIC (Starch). Too much background fluorescence from starch.

TEM but getting sections is difficult. We were able to image the cellulose
fibers using the CBH gold conjugate but not the starch using the Con A-gold
conjugate.

Osmium Tetroxide staining, saw some faint staining across the samples but
nothing that would be indicative of the starch distribution

Ideas that I have brainstormed thus far (not all of them necessarily
useful):
EDXS, XPS, XRF, Tof SIMS, RBS, TEM, EELS, Fluorescent staining, X-ray
absorption/Diffraction/Tomography, Osmium staining with SEM/TEM (again),
Confocal, IR, E-SEM, Chemical etching of the fibres, gold labelling, NMR,
Raman, Conductivity (through two plates??? Different hydration??),
Ultrasound, FRET, Multi Photon Microscopy.

If you have any thoughts, ideas, comments, techniques, papers, texts on the
subject I would very much like to hear from you. I'll even take a wizened,
gnarled old microscopist, as long as they can image starch.

Thank you for all your help.

George


George Theodossiou
Physicist / Microscopist
Microscopy and Microanalysis Laboratory

AMCOR Research and Technology
Ph: +61 3 9490 6135
Fax: +61 3 9499 4295
Mobile: 0409 568 840
email: George.Theodossiou-at-amcor.com.au


************************************************************************
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From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 02:55:50 2005



From: Klughammer :      opto-at-klughammer.de
Date: Tue, 8 Feb 2005 09:52:22 +0100
Subject: [Microscopy] Re: Coolpix 5700, Lumenera Infinity 3

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ritchie,

we offer adapters for all kind of cameras. Let me know
whether you would like to receive a quotation for an adapter
for your Nikon 5400 camera.

You can connect a digital consumer camera from

Agfa, Canon, Casio, Epson, Fuji, Hewlett-Packard,
JVC, Kodak, Leica, Minolta, Nikon,
Olympus, Pentax, Sharp, Sony, Toshiba

SLR cameras from
Canon, Fuji, Kodak, Konica, Minolta, Nikon, Olympus,
Pentax

Camcorders from
Canon, JVC, Panasonic,Sony
or threads with 30 mm, 30.5 m, 37 mm, 40.5 mm, 43 mm, 46
mm, 49 mm, 52 mm, 55 mm, 58 mm, 62 mm

to your microscope. The adapter fits to a c-mount or to a 23
mm or 30 mm eyepiece.

Our adapters are stable. Vignetting is eliminated with most
cameras because of the variation in length which can be
adjusted.

We have a list of the cameras we support - let me know when
you would like to receive it.

mfg / regards

Anneliese Schmaus
Sales Manager

klughammer gmbh
Strassbach 9
85229 Markt Indersdorf
Germany
Tel. +49 08136 6011
Fax +49 08136 7098
www.klughammer.de



RS} ------------------------------------------------------------------------------
RS} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
RS} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
RS} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
RS} -------------------------------------------------------------------------------

RS} Hi

RS} Anybody got any experience to share, either good or bad, with the
RS} Lumenera Infinity 3?

RS} Also, does anyone know a source of microscope adaptors for the Nikon
RS} Coolpix 5700?
RS} Presumably they involve also the Nikon UR-E8 adaptor.

RS} cheers and tia

RS} rtch

RS} --
RS} Ritchie Sims Ph D Phone : 64 9 3737599
RS} ext 87713
RS} Microanalyst Fax : 64 9 3737435
RS} Department of Geology email :
RS} r.sims-at-auckland.ac.nz
RS} The University of Auckland
RS} Private Bag 92019
RS} Auckland
RS} New Zealand



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 03:15:19 2005



From: gillian.2.brown-at-gsk.com
Date: Tue, 8 Feb 2005 09:12:35 +0000
Subject: [Microscopy] Re: processing loose balls of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Leona,
we have just started using very successfully a Thermo Electron
Corporation, Shandon Cytoblock Kit (REF 74010150) for applications such as
you describe.
You do need a Shandon Cytospin. The cells etc are gelled together and end
up concentrated and encased in a small disc which when removed from the
cassette can be embedded on edge in the wax mould.
I too do EM and am thinking of trying for that as it is so easy.

their web site: www.thermo.com/shandon

Cheers

Gill Brown


Histopathology Group
Asthma and Allergy Disease Biology
ri- CEDD.
GlaxoSmithKline Medicines Research Centre,
Gunnelswood Road,
STEVENAGE,
Hertfordshire.
SG1 2NY
tel. +44 (0)1438 764119
fax. +44 (0)1438 764782
email. gillian.2.brown-at-gsk.com





From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 04:29:07 2005



From: Gerd Leitinger :      gerd.leitinger-at-meduni-graz.at
Date: Tue, 08 Feb 2005 11:24:43 +0100
Subject: [Microscopy] Golgi-stained specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

Does anybody know a source for prepared slides with Golgi-stained
neurons?

I am putting together a course for students on the histology of the
nervous system, and would like to demonstrate the benefits of the
Golgi method over more classical histological methods, but I do not
have the time or know-now to prepare Golgi specimens myself.
Ideally, the slides should show neurons of the vertebrate cortex, but
could also be of other parts of the nervous system or of other animals.
I know of two companies that sell prepared slides, but one of them
(Fisher Scientific) only sells sets of slides, many of which I will not
need, and the other (Home Training Tools) does not ship outside the
US.

I would be VERY grateful for any suggestions

thank you

Gerd


Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 04:30:31 2005



From: Gareth Morgan :      Gareth.Morgan-at-labmed.ki.se
Date: Tue, 08 Feb 2005 14:01:33 +0100
Subject: [Microscopy] Re: Imaging Starch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

George

I don't know if you have tried this, but could you not experiment with
amylase digestion and either look before and after or even at what's
made soluble? I've never tried this so I'm sorry if I've stated the
obvious/impossible.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK

e-mail: malcolm.haswell-at-sunderland.ac.uk





----- Original Message -----
} From: George Theodossiou {George.Theodossiou-at-amcor.com.au}

Hi

The first thing that comes to mind is PAS with and without amylase/diastase
treatment. It will be the same as the iodine staining but the amylase
treated section will lack the starch as it is labile to the enzyme. (PAS
with/without amylase is standard for identifying glycogen in animal tissues).

The other thought is polarised light. Starch will give a Maltese Cross
birefringence. So will talc but they are morphologically different and the
clincher would be to combine PAS and polarised light.

Hope this helps

All the best

Gareth


} 1. To image the starch optically and look at its distribution
} 2. To detect the starch spectroscopically
} 3. To find a way of differentiating between various starches (wheat, tapioca
} and potato)
} 4. Quantify the starch
}
} We have tried the following in house and using external service providers:
} Iodine staining (but it also stains the cellulose, therefore differentiating
} starch and fibre is difficult)
}
} Fluorescence microscopy using the stains Calcofluor White (cellulose) and
} Con A-FTIC (Starch). Too much background fluorescence from starch.
}
} TEM but getting sections is difficult. We were able to image the cellulose
} fibers using the CBH gold conjugate but not the starch using the Con A-gold
} conjugate.
}
} Osmium Tetroxide staining, saw some faint staining across the samples but
} nothing that would be indicative of the starch distribution
}
} Ideas that I have brainstormed thus far (not all of them necessarily
} useful):
} EDXS, XPS, XRF, Tof SIMS, RBS, TEM, EELS, Fluorescent staining, X-ray
} absorption/Diffraction/Tomography, Osmium staining with SEM/TEM (again),
} Confocal, IR, E-SEM, Chemical etching of the fibres, gold labelling, NMR,
} Raman, Conductivity (through two plates??? Different hydration??),
} Ultrasound, FRET, Multi Photon Microscopy.
}
} If you have any thoughts, ideas, comments, techniques, papers, texts on the
} subject I would very much like to hear from you. I'll even take a wizened,
} gnarled old microscopist, as long as they can image starch.
}
} Thank you for all your help.
}
} George
}
}
} George Theodossiou
} Physicist / Microscopist
} Microscopy and Microanalysis Laboratory
}
} AMCOR Research and Technology
} Ph: +61 3 9490 6135
} Fax: +61 3 9499 4295
} Mobile: 0409 568 840
} email: George.Theodossiou-at-amcor.com.au
}
}
} ************************************************************************
} CAUTION - This message may contain privileged and confidential
} information intended only for the use of the addressee named above.
} If you are not the intended recipient of this message you are hereby
} notified that any use, dissemination, distribution or reproduction of
} this message is prohibited. If you have received this message in error
} please notify AMCOR immediately.
} Any views expressed in this message are those of the individual sender
} and may not necessarily reflect the views of AMCOR.
} ************************************************************************


With best wishes,

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Histo/cytopathology, Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 07:56:58 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Tue, 08 Feb 2005 08:54:29 -0500
Subject: [Microscopy] Re: processing loose balls of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Surround them with low-gelling agarose and embed a black thread in the
agarose before it gels.

At 05:05 PM 2/7/2005 -0500, Leona Cohen-Gould wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 08:02:20 2005



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Tue, 8 Feb 2005 08:00:26 -0600
Subject: [Microscopy] starch Id.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

George:
I have identified starch particles (offset powders) on our
lithographed cards and polarized microscopy revealed them the best , they
show nice German crosses (X) under crossed polars, SEM can also be used but
that's over kill . I use 3m sticky tape to remove the starch from the cards
then identify and count them if necessary . If you have a metallurgical
microscope you can also see the starch particle on the card stock but since
I have to turn the cards upside down and move it around you loose some
particles. McCrone has some nice pictures and dimensions of the different
starches in their PAE2 Particle Atlas.
I have also collected some known offset powders ( starches) from the
suppliers and used them to identify the different starches. Some starches
are easy to identify and others are difficult but it can be done from the
shapes and size ranges of the different starches.

Terry Ellis
Hallmark Cards Inc.
816-545-6573



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 08:09:50 2005



From: davebernh-at-golden.net (by way of MicroscopyListserver)
Date: Tue, 8 Feb 2005 08:08:17 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Nikon Apophot Questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (davebernh-at-golden.net) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, February 8, 2005 at 01:42:32
---------------------------------------------------------------------------

Email: davebernh-at-golden.net
Name: Dave Bernhardt

Organization: NA

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello All,
My love of fine optics has garnered me a Nikon Apophot microscope and a lot of questions. Could (would) anyone
be able to assist in the "specs" for this microscope?
The "net" searches have but ever so fleeting referece to this unit and Nikon seems to have no available info at all.
All I have been able to find out is that this microscope was manufactured in the late 60's, approx 1968! Any input on or about this microscope would be appreciated.

PS: photos are available

Thank You
Dave Bernhardt
davebernh at golden dot net

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 08:11:02 2005



From: kinder-at-cableone.net (by way of MicroscopyListserver)
Date: Tue, 8 Feb 2005 08:09:28 -0600
Subject: [Microscopy] AskAMicroscopist: help with focusing a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kinder-at-cableone.net) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, February 8, 2005 at 07:46:37
---------------------------------------------------------------------------

Email: kinder-at-cableone.net
Name: Nanette

Organization: Homeschool

Education: Undergraduate College

Location: grenada, ms

Question: Where can I find help with focusing a microscope? This is a Bristoline microscope that was purchased on Ebay. I can't seem to focus in on anything. Any help would be appreciated.
Thanks,
Nanette

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 08:18:16 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Tue, 8 Feb 2005 09:14:49 -0500
Subject: [Microscopy] Re: Imaging Starch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

George,
In your long list of every method under the lens, I was
surprised not to see polarized light. Starch is birefringent and very
lovely in polarized light. Different species grains are
distinguishable too. Of course, the cellulose is birefringent too but
it should not be too hard to distinguish long flat ribbons from beach
balls. I don't have references to hand but I bet you could get
started in Fry-Wyssling's book Submicroscopic Morphology (a gnarled
old microscopist's book for you).

Hope this helps,
Tobias
}
} Hi All,
}
} A very broad question to draw out as many thoughts and ideas as possible.
} We are at it again in the box factory trying to image starch in paper and
} board and I am seeking every scrap of information I can lay my hands on. It
} seems we visit this issue every year because we can never seem to find a
} satisfactory solution. There are several things we would like to achieve:
}
} 1. To image the starch optically and look at its distribution
} 2. To detect the starch spectroscopically
} 3. To find a way of differentiating between various starches (wheat, tapioca
} and potato)
} 4. Quantify the starch
}
} We have tried the following in house and using external service providers:
} Iodine staining (but it also stains the cellulose, therefore differentiating
} starch and fibre is difficult)
}
} Fluorescence microscopy using the stains Calcofluor White (cellulose) and
} Con A-FTIC (Starch). Too much background fluorescence from starch.
}
} TEM but getting sections is difficult. We were able to image the cellulose
} fibers using the CBH gold conjugate but not the starch using the Con A-gold
} conjugate.
}
} Osmium Tetroxide staining, saw some faint staining across the samples but
} nothing that would be indicative of the starch distribution
}
} Ideas that I have brainstormed thus far (not all of them necessarily
} useful):
} EDXS, XPS, XRF, Tof SIMS, RBS, TEM, EELS, Fluorescent staining, X-ray
} absorption/Diffraction/Tomography, Osmium staining with SEM/TEM (again),
} Confocal, IR, E-SEM, Chemical etching of the fibres, gold labelling, NMR,
} Raman, Conductivity (through two plates??? Different hydration??),
} Ultrasound, FRET, Multi Photon Microscopy.
}
} If you have any thoughts, ideas, comments, techniques, papers, texts on the
} subject I would very much like to hear from you. I'll even take a wizened,
} gnarled old microscopist, as long as they can image starch.
}
} Thank you for all your help.
}
} George
}
}
} George Theodossiou
} Physicist / Microscopist
} Microscopy and Microanalysis Laboratory
}
} AMCOR Research and Technology
} Ph: +61 3 9490 6135
} Fax: +61 3 9499 4295
} Mobile: 0409 568 840
} email: George.Theodossiou-at-amcor.com.au
}
}
} ************************************************************************
} CAUTION - This message may contain privileged and confidential
} information intended only for the use of the addressee named above.
} If you are not the intended recipient of this message you are hereby
} notified that any use, dissemination, distribution or reproduction of
} this message is prohibited. If you have received this message in error
} please notify AMCOR immediately.
} Any views expressed in this message are those of the individual sender
} and may not necessarily reflect the views of AMCOR.
} ************************************************************************


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 08:52:09 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 8 Feb 2005 08:49:42 -0600
Subject: [Microscopy] Imaging Starch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

George,

I have never tried to image starch in paper or board, and I'm sceptical
that intact starch grains would survive the manufacturing process in any
quantity (but I don't know that for sure).

Starch grains are often optically visualized using a standard optical
microscope and are differentiated by size, shape, melting point,
solubility in various reagents, and properties of the "Maltese Cross".
The latter is visible using crossed polarizers. For more information on
starch identification methods and keys, check out "Reichert, E.T. 1913.
The Differentiation and Specificity of Starches in Relation to Genera,
Species, Etc. Washington. Carnegie Institute", if you can find a copy
anywhere. It's old, but it's almost unique and represents a HUGE amount
of basic work, much of which is still useful and relevant. Obviously it
doesn't cover newer technologies.

Also, for a wealth of references and basic info on starch research and
identification go to http://www.siu.edu/~ebl/amylose.htm.

Hope this gets you started.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








-----Original Message-----
} From: George Theodossiou [mailto:George.Theodossiou-at-amcor.com.au]
Sent: Tuesday, February 08, 2005 12:52 AM
To: 'Microscopy-at-msa.microscopy.com'

Hi All,

A very broad question to draw out as many thoughts and ideas as
possible.
We are at it again in the box factory trying to image starch in paper
and board and I am seeking every scrap of information I can lay my hands
on. It seems we visit this issue every year because we can never seem
to find a satisfactory solution. There are several things we would like
to achieve:

1. To image the starch optically and look at its distribution 2. To
detect the starch spectroscopically 3. To find a way of differentiating
between various starches (wheat, tapioca and potato) 4. Quantify the
starch

We have tried the following in house and using external service
providers:
Iodine staining (but it also stains the cellulose, therefore
differentiating starch and fibre is difficult)

Fluorescence microscopy using the stains Calcofluor White (cellulose)
and Con A-FTIC (Starch). Too much background fluorescence from starch.


TEM but getting sections is difficult. We were able to image the
cellulose fibers using the CBH gold conjugate but not the starch using
the Con A-gold conjugate.

Osmium Tetroxide staining, saw some faint staining across the samples
but nothing that would be indicative of the starch distribution

Ideas that I have brainstormed thus far (not all of them necessarily
useful):
EDXS, XPS, XRF, Tof SIMS, RBS, TEM, EELS, Fluorescent staining, X-ray
absorption/Diffraction/Tomography, Osmium staining with SEM/TEM (again),
Confocal, IR, E-SEM, Chemical etching of the fibres, gold labelling,
NMR, Raman, Conductivity (through two plates??? Different hydration??),
Ultrasound, FRET, Multi Photon Microscopy.

If you have any thoughts, ideas, comments, techniques, papers, texts on
the subject I would very much like to hear from you. I'll even take a
wizened, gnarled old microscopist, as long as they can image starch.

Thank you for all your help.

George


George Theodossiou
Physicist / Microscopist
Microscopy and Microanalysis Laboratory

AMCOR Research and Technology
Ph: +61 3 9490 6135
Fax: +61 3 9499 4295
Mobile: 0409 568 840
email: George.Theodossiou-at-amcor.com.au


************************************************************************
CAUTION - This message may contain privileged and confidential
information intended only for the use of the addressee named above.
If you are not the intended recipient of this message you are hereby
notified that any use, dissemination, distribution or reproduction of
this message is prohibited. If you have received this message in error
please notify AMCOR immediately.
Any views expressed in this message are those of the individual sender
and may not necessarily reflect the views of AMCOR.
************************************************************************





From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 09:47:35 2005



From: Jan Factor :      jfactor-at-ns.purchase.edu
Date: Tue, 08 Feb 2005 10:47:21 -0500
Subject: [Microscopy] Re: Re: processing loose balls of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was going to make the same kind of suggestion, but using a strip of
thin paper on either side of the ball of cells so you can locate them in
the block. The paper can either be trimmed away or sectioned through.
--Jan Factor

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College, State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



Greg Erdos wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Surround them with low-gelling agarose and embed a black thread in the
} agarose before it gels.
}
} At 05:05 PM 2/7/2005 -0500, Leona Cohen-Gould wrote:
}
}
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} }
} } Hi All,
} } I have just received a request to process balls of what had been
} } mouse stem cells which have now been differentiated into
} } cardiomyocytes. They are loose, spongy balls of cells that fall
} } apart when prodded. The PI would like them embedded in paraffin and
} } sectioned for light microscopy. If these were for EM, I'd be
} } perfectly comfortable with the whole thing, but I am a bit boggled
} } about how to handle infiltration and embedding in wax...they are
} } essentially transparent and will disappear.
} } I know that there was a thread about a similar topic a short time
} } ago, where is was suggested that the samples in question be stained
} } before embedding. The problem here is that the PI would like to be
} } able to do a variety of staining protocols on the resultant slides.
} } Ideas?
} } thanks,
} } Lee
} } --
} } Leona Cohen-Gould, M.S.
} } Sr. Staff Associate
} } Director, Electron Microscopy & Histology Core Facility
} } Manager, Optical Microscopy Core Facility
} } Joan & Sanford I. Weill Medical College
} } of Cornell University
} } voice (212)746-6146
} } fax (212)746-8175
}
}
} Gregory W. Erdos Ph.D.
} Assistant Director, Biotechnology Program
} Scientific Director, Electron Microscopy
} P.O. Box 118525
} 217 Carr Hall
} University of Florida
} Gainesville, FL 32611
} gwe-at-ufl.edu
} 352-392-1295
} fax- 352-846-0251
}


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 11:23:16 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 8 Feb 2005 09:34:53 -0800
Subject: [Microscopy] Lattice constant of gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,
Thanks to all who replied. I was able to find the appropriate
reference.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 11:35:02 2005



From: Barbara :      bfoster-at-mme1.com
Date: Tue, 08 Feb 2005 11:31:45 -0600
Subject: [Microscopy] Re: Imaging Starch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear George,

Nothing fancy, but have you tried Polarized light? Starch exhibits a unique "Maltese Cross" pattern on a bright white background. While you will also get a Pol response from the cellulose, it will be dramatically different. You should be able to segment (for your quantification) either on the pattern or on the bright white areas of a specific size (depending on which software you have, you can use one of the "Fill Hole" algorithms to eliminate the areas of the cross)

As for distinguishing between different starches, I would think that simple FT-IR would be the key. I suspect there are slightly different IR signatures. The easiest implementation would be to add SensIR's IlluminatIR to an upright microscope that you already have. Also, if you'd like to learn more about this technique, I have a PDF of an article I wrote on IlluminatIR several years ago. Just contact me off-line.

If you call Greg Durdock or Dave Johnson at SensIR (203-207-9708), they should be able to arrange to have some samples run for you to tell you whether or not this approach will work.

Hope this is helpful.

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.

Caveat: MME helped to launch IlluminatIR but has no financial interest in this product.

At 12:51 AM 2/8/2005, George Theodossiou wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 11:36:28 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Tue, 8 Feb 2005 11:34:10 -0600
Subject: [Microscopy] MT-5000 Ultramicrotome Value

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone in this group know what the market value of a used MT-5000
ultramicrotome is worth?



Garry Burgess
Charge Technologist
Department of Electron Microscopy
Health Sciences Center
Winnipeg, Canada

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 11:53:30 2005



From: Barbara :      bfoster-at-mme1.com
Date: Tue, 08 Feb 2005 11:30:50 -0600
Subject: [Microscopy] Re: Imaging Starch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear George,

Nothing fancy, but have you tried Polarized light? Starch exhibits a unique "Maltese Cross" pattern on a bright white background. While you will also get a Pol response from the cellulose, it will be dramatically different. You should be able to segment (for your quantification) either on the pattern or on the bright white areas of a specific size (depending on which software you have, you can use one of the "Fill Hole" algorithms to eliminate the areas of the cross)

As for distinguishing between different starches, I would think that simple FT-IR would be the key. I suspect there are slightly different IR signatures. The easiest implementation would be to add SensIR's IlluminatIR to an upright microscope that you already have. Also, if you'd like to learn more about this technique, I have a PDF of an article I wrote on IlluminatIR several years ago. Just contact me off-line.

If you call Greg Durdock or Dave Johnson at SensIR (203-207-9708), they should be able to arrange to have some samples run for you to tell you whether or not this approach will work.

Hope this is helpful.

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.

Caveat: MME helped to launch IlluminatIR but has no financial interest in this product.

At 12:51 AM 2/8/2005, George Theodossiou wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 11:56:37 2005



From: Barbara :      bfoster-at-mme1.com
Date: Tue, 08 Feb 2005 11:53:20 -0600
Subject: [Microscopy] Re: AskAMicroscopist: help with focusing a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Nanette

Several thoughts come to mind:
The first is that there is a prism mis-aligned. I'm not familiar with this line of microscope: does it have an adjustable tube lens that raises the eyepiece up and down a substantial amount? If so, you may need to experiment to find the best setting.

The second thought is that you are mis-aligned (no disrespect intended). Are you sitting really close to the microscope? If so, try sitting close then slowly moving you head back from the eyepieces.

Let us know if either of these adjustments work.

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.





At 08:09 AM 2/8/2005, kinder-at-cableone.net wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 11:59:11 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 08 Feb 2005 12:58:13 -0800
Subject: [Microscopy] Re: processing loose balls of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To find the ball of cells you could:
1. put some eosin in the dehydrating alcohols so you can see the cells.
Osmium might also work.
2. put the balls in gelatin or agar inside a tube you can see, a cross
section of aorta or small intestine might be worth a try.

Geoff

Leona Cohen-Gould wrote:

} Hi All,
} I have just received a request to process balls of what had been mouse
} stem cells which have now been differentiated into cardiomyocytes.
} They are loose, spongy balls of cells that fall apart when prodded.
} The PI would like them embedded in paraffin and sectioned for light
} microscopy. If these were for EM, I'd be perfectly comfortable with
} the whole thing, but I am a bit boggled about how to handle
} infiltration and embedding in wax...they are essentially transparent
} and will disappear.
} I know that there was a thread about a similar topic a short time ago,
} where is was suggested that the samples in question be stained before
} embedding. The problem here is that the PI would like to be able to
} do a variety of staining protocols on the resultant slides.
} Ideas?
} thanks,
} Lee


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 12:51:52 2005



From: Anjeanette Ormonde :      Anjeanette.Ormonde-at-unilever.com
Date: Tue, 8 Feb 2005 13:49:38 -0500
Subject: [Microscopy] Cryo-EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Our lab has begun exploring cryo-SEM to look at the microstructure of
surfactant systems and other similar types of samples. However, as I have no
prior experience I'm never sure exactly what I'm looking at and what methods of
sample prep are best. It has been suggested that I form an outside
collaboration that can help us improve our cryo-capabilities and provide a much
needed "professional opinion". So, I'm looking for recommendations.
This person would probably have to give a talk to a small group of scientists
at some point but would primarily be working one-on-one with me. It isn't
exactly clear at this point how the relationship would work out but I'm
thinking a visit to our lab for 1 or 2 days then more frequent e-mail
conversations. If all goes well there may be an annual visit of about a day.
With the collaborative relationships I've seen in the past, the outside
parties are typically in a university setting. If you, or anyone you know,
might be interested in such a collaboration please let me know offline. The
only requirements are that they are recognized as an expert in the field and
have surfactant experience. I would need a CV showing any publications and
references would be nice.

Thanks,
Angie




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 12:55:29 2005



From: Tamara Howard :      thoward-at-unm.edu
Date: Tue, 8 Feb 2005 11:53:13 -0700 (MST)
Subject: [Microscopy] Re: processing loose balls of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lee - Could you make a giant Formvar sandwich with them? I'm worried that
they might fall apart when adding to agarose; does the PI need them as
intact balls?

Sounds like fun (picture me rolling my eyes).

Tamara

On Mon, 7 Feb 2005, Leona Cohen-Gould wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi All,
} I have just received a request to process balls of what had been mouse stem
} cells which have now been differentiated into cardiomyocytes. They are
} loose, spongy balls of cells that fall apart when prodded. The PI would like
} them embedded in paraffin and sectioned for light microscopy. If these were
} for EM, I'd be perfectly comfortable with the whole thing, but I am a bit
} boggled about how to handle infiltration and embedding in wax...they are
} essentially transparent and will disappear.
} I know that there was a thread about a similar topic a short time ago, where
} is was suggested that the samples in question be stained before embedding.
} The problem here is that the PI would like to be able to do a variety of
} staining protocols on the resultant slides.
} Ideas?
} thanks,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy & Histology Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 13:16:35 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 08 Feb 2005 14:14:01 -0500
Subject: [Microscopy] Re: processing loose balls of cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tamara,
Yes, he'd like them intact. Luckily, he has dozens of them to play
with, so I will try a variety of things. I'm hoping that they have a
little structural integrity once they are fixed in pfa. It'll be
interesting.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 13:24:38 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 08 Feb 2005 14:22:33 -0500
Subject: [Microscopy] cell balls...thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you all for your helpful ideas. The leading candidates to try are:

1. encapsulating the cells in gelatin (staining either the cells or
the gelatin)
2. eosin staining the free-floating balls of cells during the last
dehyration steps so that they can be seen in the wax
3. putting the cell balls into either a "tea bag" within an embedding
cassette, or using the very fine mesh, divided cassettes and
processing as usual.

Luckily for me the PI has many, many of these little things, and is
willing to let me try a number of things.

As always, "the List" has come through again.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 13:26:35 2005



From: Terry E Ellis :      tellis2-at-hallmark.com
Date: Tue, 8 Feb 2005 13:24:45 -0600
Subject: [Microscopy] reference for starch in paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

George:
In case you are concerned about the starch in the paper (internal
size, surface size, adhesive in pigment coating ) the book Analysis of
Paper by
B.L. Browning describes several wet chemistry methods to do so. For a more
modern approach see Surface Analysis of Paper by Terrance E. Conners and
Suji Banerjee, I think this book is less useful than the one by B. L.
Browning but it is interesting and has useful info on other components on
and in paper.
Terry E Ellis
Hallmark Cards Inc.
816-545-6573



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 13:26:51 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 08 Feb 2005 14:24:01 -0500
Subject: [Microscopy] Re: MT-5000 Ultramicrotome Value

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
}
} Does anyone in this group know what the market value of a used MT-5000
ultramicrotome is worth?

It makes a very effective paperweight. Remember, that model is what
put Dupont out of the microtome business.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 15:10:03 2005



From: Phaedra McGuinness :      scanning-at-fams.org
Date: Tue, 8 Feb 2005 16:25:07 -0500
Subject: [Microscopy] SCANNING 2005 Abstract Deadline Extended

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well...Lee has a more environmentally friendly idea...mine was it also makes
a great boat anchor ;-)



Al Coritz
EMS/Diatome USA

----- Original Message -----
} From: "Garry Burgess" {GBurgess-at-exchange.hsc.mb.ca}
To: "MSA listserver" {Microscopy-at-msa.microscopy.com}
Sent: Tuesday, February 08, 2005 12:34 PM

Dear Listers:

Re: SCANNING 2005, April 5-7 in Monterey, California

**Please note the abstract deadline for submissions has been extended
to February 28. **

Please visit www.scanning.org for complete meeting details and the
updated preliminary program.

See you in beautiful Monterey!


Best regards,


Phaedra McGuinness
Managing Editor
SCANNING, The Journal of Scanning Microscopies
P.O. Box 485
Mahwah, NJ 07430
Tel: (201) 818-1010 * Fax: (201) 818-0086 *email: scanning-at-fams.org *
Web: www.scanning.org



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 16:39:28 2005



From: George Theodossiou :      George.Theodossiou-at-amcor.com.au
Date: Wed, 9 Feb 2005 11:43:26 +1100
Subject: [Microscopy] Imaging Starch Part 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would not be so harsh. I´m doing service on DuPont / RMC in Germany and my
experience had been that changing the lubricants and putting a day of work
into the microtome (older types from MT-5000 to MT-7000) would give some
more years of working time...
Sure: there are some microtomes not worth repairing...
Selling it depends on how reliable it cuts, how even the stages are working,
how equal the sections are in thickness... But I think you know this...

Best regards,
Stefan Diller



----- Original Message -----
} From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu}
To: "Garry Burgess" {GBurgess-at-exchange.hsc.mb.ca} ; "MSA listserver"
{Microscopy-at-msa.microscopy.com}
Sent: Tuesday, February 08, 2005 8:24 PM

Hi,

Thank you for all the responses. I didn't provide enough background
information in my original email so here is a bit more.

Optical microscopy using polarised light and exploiting the Maltese Cross
birefringence is great for 'uncooked' starch ie granules, but in paper and
cardboard the starch is 'cooked' and the granules which can be thought of as
a ball of string unwinds. The end result is a carbohydrate chain that glues
the cellulose fibres together and contributes to the strength and rigidity.


In electron microscopy we have the same issue. The paper and cardboard can
be sectioned by appropriate means and coated but cooked starch is nigh on
impossible to see amongst the cellulose fibres, fillers (CaCO3 and Clay),
optical brightners (TiO2) etc without tagging it in some way.

Amylase and diastase are enzymes that are used to chop up the carbohydrate
chains. The trick is to chop the starch up to lengths that give good
strength. If it continues to far and chops the chain down to its sugar
units, then we lose viscocity and the strength of the final product is
compromised.

The goal for us is to image the carbohydrate chains (cooked starch) in the
finished product (paper, cardboard, corrugated cardboard) to meet our four
points below. (for rolls 3-7 m wide and several kilometers long, but that's
a sampling problem)
1. To image the starch optically and look at its distribution
2. To detect the starch spectroscopically
3. To find a way of differentiating between various starches (wheat, tapioca
and potato)
4. Quantify the starch

Thanks again for all your responses, I eagerly await the next round.

Regards
George

PS Philip, in melbourne on tha banks of the Yarra river. Great native
parklands nearby, excellent for running after work. Great city, with best
little Italian café...Pellegrinis. ;-)


George Theodossiou
Physicist / Microscopist
Microscopy and Microanalysis Laboratory

AMCOR Research and Technology
Ph: +61 3 9490 6135
Fax: +61 3 9499 4295
Mobile: 0409 568 840
email: George.Theodossiou-at-amcor.com.au


************************************************************************
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information intended only for the use of the addressee named above.
If you are not the intended recipient of this message you are hereby
notified that any use, dissemination, distribution or reproduction of
this message is prohibited. If you have received this message in error
please notify AMCOR immediately.
Any views expressed in this message are those of the individual sender
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From MicroscopyL-request-at-ns.microscopy.com Tue Feb 8 20:34:25 2005



From: Damian Neuberger :      neuberger1234-at-comcast.net
Date: Tue, 8 Feb 2005 20:32:02 -0600
Subject: [Microscopy] RE: Imaging Starch & Part 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

George,

When you mention using iodine, I assume you are using a weak solution of
iodine-potassium iodide. (0.3g iodine, 1.5g potassium iodide, 100cc water.
The starch grains (depending on how modified they are from the
paper/cardboard mfg) will stain dark blue to black and the color will
disappear upon heating and reappear upon cooling. I wonder if you stain
with Safranin O first to get the cellulose in the "fibers" and then use the
Isub2KI for the solubilized starch.

Now reading your Imaging Starch Part 2, I see that you really want to see if
the starch has been digested too far, losing viscosity and strength. Are
you suggesting that you want to literally look at the dispersed cellulose
chain fragments amongst the plant fibers? That sounds like TEM method and
if you are looking at 30m wide rolls that are several km long your
statistics becomes an issue. Getting wisdom from a real old wizened and
yellowed Plant Microtechnique textbook by Donald Alexander Johansen (McGraw
Hill) and long out of print (1940). There is a Chapter on Microchemical
Methods - Carbohydrates - Sugars. It has tests for Sucrose, fructose,
maltose, amylodextrin and glucose. I wonder if you could run samples
testing for these components to see if the quantity of these other sugars
would give you an idea of how much the starch has been digested. I
understand that hydrolyzing the cellulose in the fibers, tracheids, and
vessel elements (the latter if only you are using angiosperm woods in the
mfg process) would presumably require 70-75% sulfuric acid or HCl (specific
gravity ???). Are you able to "macerate" the samples and separate the
fibers from the other particles?

I assume you have already obtained some of the three starch grains used in
the mfg process and have done a partial digestion and characterized those
first as well as determine whether or not you can also see the digestion
products.

If you want to check out the microchemical stains, safranin stains, I can
scan the pages for you and email them. Also a good reference for paper
fibers and starch grains is the McCrone Atlas and I have the entire series
on CD.

Damian Neuberger, Ph.D. (botany :-)
Microscopy and Digital Photography Consultant
2416 Covert Rd
Glenview IL 60025
Tel: (847) 998-8574
email: neuberger1234-at-comcast.net {mailto:neuberger1234-at-comcast.net}


Hi All,

A very broad question to draw out as many thoughts and ideas as possible.
We are at it again in the box factory trying to image starch in paper and
board and I am seeking every scrap of information I can lay my hands on. It
seems we visit this issue every year because we can never seem to find a
satisfactory solution. There are several things we would like to achieve:

1. To image the starch optically and look at its distribution
2. To detect the starch spectroscopically
3. To find a way of differentiating between various starches (wheat, tapioca
and potato)
4. Quantify the starch

We have tried the following in house and using external service providers:
Iodine staining (but it also stains the cellulose, therefore differentiating
starch and fibre is difficult)

Fluorescence microscopy using the stains Calcofluor White (cellulose) and
Con A-FTIC (Starch). Too much background fluorescence from starch.

TEM but getting sections is difficult. We were able to image the cellulose
fibers using the CBH gold conjugate but not the starch using the Con A-gold
conjugate.

Osmium Tetroxide staining, saw some faint staining across the samples but
nothing that would be indicative of the starch distribution

Ideas that I have brainstormed thus far (not all of them necessarily
useful):
EDXS, XPS, XRF, Tof SIMS, RBS, TEM, EELS, Fluorescent staining, X-ray
absorption/Diffraction/Tomography, Osmium staining with SEM/TEM (again),
Confocal, IR, E-SEM, Chemical etching of the fibres, gold labelling, NMR,
Raman, Conductivity (through two plates??? Different hydration??),
Ultrasound, FRET, Multi Photon Microscopy.

If you have any thoughts, ideas, comments, techniques, papers, texts on the
subject I would very much like to hear from you. I'll even take a wizened,
gnarled old microscopist, as long as they can image starch.

Thank you for all your help.

George





From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 07:11:25 2005



From: Miller, Shea :      MILLERS-at-agr.gc.ca
Date: Wed, 9 Feb 2005 08:12:06 -0500
Subject: [Microscopy] LM - GFP in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all:

I have a user who is trying to look at GFP expression in plant leaves. We are having a hard time with the fluorescence from the chlorophyll masking everything else. Is there some way to quench the chlorophyll, without killing the GFP?



thanks in advance

shea


Dr. S. Shea Miller
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1760
Facsimile/Télécopieur: 613-759-1701
Rm 2068 K.W. Neatby Bldg
960 Carling Ave.
Central Experimental Farm
Ottawa, ON
K1A 0C6
millers-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 08:40:18 2005



From: R. Howard Berg :      rhberg-at-danforthcenter.org
Date: Wed, 9 Feb 2005 08:40:37 -0600
Subject: [Microscopy] Re: LM - GFP in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Shea,


Try keeping the excitation power as low as possible. When we image GFP
in leaves the chlorophyll starts getting in the way when it is emitting
enough light that this emission spills into the GFP channel.

Howard


On Feb 9, 2005, at 7:12 AM, Miller, Shea wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Hi all:
}
} I have a user who is trying to look at GFP expression in plant
} leaves. We are having a hard time with the fluorescence from the
} chlorophyll masking everything else. Is there some way to quench the
} chlorophyll, without killing the GFP?
}
}
}
} thanks in advance
}
} shea
}
}
} Dr. S. Shea Miller
} Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
} Telephone/Téléphone: 613-759-1760
} Facsimile/Télécopieur: 613-759-1701
} Rm 2068 K.W. Neatby Bldg
} 960 Carling Ave.
} Central Experimental Farm
} Ottawa, ON
} K1A 0C6
} millers-at-agr.gc.ca
}
} Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire
} Canada
}
}
}
}
R. Howard Berg, Ph.D.
Director, Integrated Microscopy Facility
Danforth Plant Science Center
975 N. Warson Rd.
St. Louis, MO 63132

ph 314-587-1261 fx 314-587-1361 cell 314-378-2409
rhberg-at-danforthcenter.org www.danforthcenter.org
visit this educational resource: http://www.danforthcenter.org/Cells/




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 08:41:44 2005



From: dave.piston-at-Vanderbilt.Edu (by way of MicroscopyListserver)
Date: Wed, 9 Feb 2005 08:43:06 -0600
Subject: [Microscopy] viaWWW: 6th International Weber Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dave.piston-at-Vanderbilt.Edu)
from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, February 9, 2005 at 08:38:07
---------------------------------------------------------------------------

Email: dave.piston-at-Vanderbilt.Edu
Name: Dave Piston

Organization: Vanderbilt

Title-Subject: [Microscopy] [Filtered] MListserver: 6th International Weber Symposium

Question:
Announcement and Call for Papers: 6th International Weber Symposium on
Innovative Fluorescence Methodologies in Biochemistry and Medicine; July
22-28, 2005, Kauai, Hawaii. Check out the www site:
http://lfd.uiuc.edu/weber/

This symposium series honors the fundamental and far-reaching
contributions of Professor Gregorio Weber (1916-1997). It provides a
current overview of modern fluorescence methodologies and applications
in the biological and medical sciences. As in the past six symposia,
speakers will describe state-of-the-art and emerging technologies used
in their research programs. Topics addressed will include multiphoton
fluorescence microscopy, confocal microscopy, time-resolved fluorescence
instrumentation, single molecule studies, fluctuation correlation
spectroscopy, fluorescence imaging spectroscopy, fluorescence resonance
energy transfer, macromolecular (nucleic acids and proteins)
interactions, membrane dynamics, and quantitative cellular dynamics.
The symposium will close the evening of Wednesday, July 27th, with a
spectacular Hawaiian Luau, specifically designed for attendees of the
Weber Symposium.

REGISTRATION FEES: Professional = $400, Student/Guest = $200

Meeting will be held at the Kauai Marriott Resort and Beach Club,
located on Kalapaki Beach, Lihue, Hawaii, on the Island of Kauai
(1-800-220-2925). We have negotiated a room rate of $195 (plus 11.416%
tax) per night, applicable for the dates of the symposium, plus 3 days
before and after. Please identify yourself as a participant of the "6th
Innovative Fluorescence Methodologies in Biochemistry & Medicine
Symposium" in order to receive the special symposium rate.

Further information can be obtained from any of the organizers: David M.
Jameson, (djameson-at-hawaii.edu) or Enrico Gratton (lfd-at-uiuc.edu) or the
Conference Secretary, Julia Wright (wrightj-at-uiuc.edu).

Aloha!
---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 09:01:39 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 09 Feb 2005 10:02:05 -0500
Subject: [Microscopy] Re: LM - GFP in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Shea,
The "plant people" may have an answer for you about quenching he
chlorophyll. My suggestion, from the hardware end, would be to find
out if anyone around you has a confocal microscope that can do
spectral separation ( eg: either the Zeiss Meta or the Leica). These
instruments can discriminate bands as narrow as 10nm and so can
separate out fluorescent signals that have similar spectra.
Lee

no commercial interest in either Zeiss or Leica...I'm just a happy
microscopist.
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 09:50:31 2005



From: Nunnemacher, Gretl :      Gretl.Nunnemacher-at-bhs.org
Date: Wed, 9 Feb 2005 10:51:10 -0500
Subject: [Microscopy] digital imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are a clinical electron microscopy laboratory that needs to start using digital imaging. We currently use a JEOL 1210 microscope. It has an older, side mounted CCD camera. The images were unsatisfactory and the process cumbersome, so we are still using 4489 EM film and an Ilford print processor. I would like to inquire about a good negative scanner and/or a better digital imaging system. Can anyone give me some advise? I woud appreciate any help I can get. Thanks, Gretl Nunnemacher


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From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 09:52:51 2005



From: James Chalcroft :      jchalcro-at-neuro.mpg.de
Date: Wed, 9 Feb 2005 16:53:57 +0100
Subject: [Microscopy] LM - GFP in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Shea,

Are you sure that the problem is coming from the chlorophyll?
Chlorophyll is supposed to fluoresce around 665nm whereas your GFP should emit with a peak just over 500nm. Any reasonable suppression filter should be able to deal with that!
Perhaps you have an autofluorescence problem here?
If chlorophyll is really the problem, how about looking at etiolated leaf tissue?
Best wishes,

Jim

-----Original Message-----
} From: Miller, Shea [mailto:MILLERS-at-agr.gc.ca]
Sent: Wednesday, February 09, 2005 2:12 PM
To: microscopy-at-microscopy.com

Hi all:

I have a user who is trying to look at GFP expression in plant leaves. We are having a hard time with the fluorescence from the chlorophyll masking everything else. Is there some way to quench the chlorophyll, without killing the GFP?



thanks in advance

shea


Dr. S. Shea Miller
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1760
Facsimile/Télécopieur: 613-759-1701
Rm 2068 K.W. Neatby Bldg
960 Carling Ave.
Central Experimental Farm
Ottawa, ON
K1A 0C6
millers-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada






From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 10:22:15 2005



From: JLaGoy :      Jane.LaGoy-at-bodycote.com
Date: Wed, 9 Feb 2005 11:36:01 -0500
Subject: [Microscopy] refurbishing Nikon microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello listers,

We have a Nikon Optiphot light microscope, still in good shape except for
spots that are either somewhere on some unreachable lens or are defects
(pits?) in a lens or mirror. The last time it was routinely cleaned by a
professional nothing they did could eliminate the fuzzy spots in pictures.
We also have a Nikon Epiphot with similar problems but not as bad. My
question is, can anyone recommend a Nikon microscope repair shop, or someone
that refurbishes these microscopes that would have parts for them? We have
some stereology work coming up that I won't be able to do accurately if I
can't get rid of the spots!

Thank you,

Jane

Jane L. LaGoy
R&D Engineer
Bodycote HIP
155 River Street
Andover, MA 01810
978-470-1620 x429
FAX 978-475-2951
jane.lagoy-at-bodycote.com



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 10:50:04 2005



From: Mark Grimson :      mark.grimson-at-ttu.edu
Date: Wed, 09 Feb 2005 10:45:01 -0600
Subject: [Microscopy] Kleinschmidt DNA spreading protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello, I was looking for a detailed protocol of the Kleinschmidt DNA
spreading technique. I have never done this technique before, so any tips
or tricks would be appreciated as well. Thanks in advance, Mark




Mark J. Grimson Ph.D
Department of Biological Sciences
Texas Tech University
Lubbock, TX 79409-3131

806-742-2704 (phone)
806-742-2963 (fax)
mark.grimson-at-ttu.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 11:14:53 2005



From: Daniel Shaw Porter :      danielshawporter-at-uky.edu
Date: Wed, 09 Feb 2005 12:14:05 -0500
Subject: [Microscopy] Embedding bone procedure for SEM and TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
Does anyone know how to embed and stain rat bone for SEM and TEM use? What is the procedure? Also I would like if possible to keep the bone calcified.
Thanks
Daniel S. Porter
Grad Student at the
University of Kentucky





From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 12:17:37 2005



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 9 Feb 2005 10:18:05 -0800 (PST)
Subject: [Microscopy] Re: digital imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A good scanner is an Epson Perfection 4870 photo. Great for negatives,
but you may need to build a holder out of posterboard for TEM negatives.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Wed, 9 Feb 2005, Nunnemacher, Gretl wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} We are a clinical electron microscopy laboratory that needs to start
} using digital imaging. We currently use a JEOL 1210 microscope. It has
} an older, side mounted CCD camera. The images were unsatisfactory and
} the process cumbersome, so we are still using 4489 EM film and an Ilford
} print processor. I would like to inquire about a good negative scanner
} and/or a better digital imaging system. Can anyone give me some advise?
} I woud appreciate any help I can get. Thanks, Gretl Nunnemacher
}
}
} -----------------------------------------
} CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at (413) 794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health System's privacy policy, please visit our Internet web site at http://www.baystatehealth.com.
}
}


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 12:43:38 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Wed, 09 Feb 2005 13:53:28 -0500
Subject: [Microscopy] MSA Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You could try contacting Phil Hutcheson at Arc Micro Optics,
arcmicro-at-mikrotec.com, 859-498-1345.

Jeff Stewart
Metallographic Laboratory Manager
Stern-Leach Company
49 Pearl Street
Attleboro, MA 02703
508-222-7400 x1329
----- Original Message -----
} From: "JLaGoy" {Jane.LaGoy-at-bodycote.com}
To: "Microscopy Listserver (E-mail)" {Microscopy-at-MSA.Microscopy.com}
Sent: Wednesday, February 09, 2005 11:36 AM

We have reduced the prices on many of the MSA videos
(DVDs). These are the older ones that may be out of date to one degree or
another. Most, however, still contain valuable information, especially for
beginners. We hope that these lower prices will allow more students to
take advantage of our archives. The full listing of DVD's and the new
price schedule can be found at the following URL:
http://www.biotech.ufl.edu/sems/newtape00.htm

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 13:21:09 2005



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 9 Feb 2005 11:18:27 -0800
Subject: [Microscopy] Ultramicrotome drive belt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

I just sat down to do some sectioning with our antique ulstramicrotome and
it was a no go.

I took the cover off and found the 'drive belt' broken. It looks like a
simple O-ring to me and I was just going to find one around here, but I
thought I would check the group for advice.

This is an AO Ultracut, circa 1981. Getting the old, broken belt off was
easy, putting a new one on might be a little more challenging. Any
experience out there?

Also, when I went to the catalog to size and price a replacement, I was
knocked out by the number of choices, both size and material. I can get the
size figured out, but any advice on material? Buna-N, Viton, Silicone and
EPDM seem to be the main choices.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 14:01:10 2005



From: David Knecht :      david.knecht-at-uconn.edu
Date: Wed, 9 Feb 2005 14:45:07 -0500
Subject: [Microscopy] Re: LM - GFP in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I haven't done this myself, but it may qualify for a relatively low
tech solution. If you also acquire images in a non-GFP emission
wavelength channel, you should simply be able to subtract out the
chlorophyll background since it is likely much broader in emission
profile than GFP. Once you know the % crosstalk of the probe under
your conditions, this should be fairly straightforward to do for all
images. Dave


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd. U-3125
Storrs, CT 06269-3125
knecht-at-uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 14:17:03 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Wed, 09 Feb 2005 12:16:58 -0800
Subject: [Microscopy] Re: Re: digital imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Epson 4870 scanner comes with a 4 x 5 holder which holds two of
them. Turn the 3 1/4" x 4" TEM negs sideways and they fit perfectly.
If you are super critical, one can cut a piece of exposed TEM film to
fit the little extra that the TEM film doesn't cover. Works great.
Have used it for years with the duo Agfa 2500 and now the Epson 4870.

Judy

Judy Murphy, PhD
Microscopy, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net

Gordon Vrololjak wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} A good scanner is an Epson Perfection 4870 photo. Great for
} negatives, but you may need to build a holder out of posterboard for
} TEM negatives.
} Gordon
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
}
} Gordon Ante Vrdoljak Electron
} Microscope Lab
} AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini
} Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA
} 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
} On Wed, 9 Feb 2005, Nunnemacher, Gretl wrote:
}
} }
} }
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} }
} } We are a clinical electron microscopy laboratory that needs to start
} } using digital imaging. We currently use a JEOL 1210 microscope. It
} } has an older, side mounted CCD camera. The images were
} } unsatisfactory and the process cumbersome, so we are still using 4489
} } EM film and an Ilford print processor. I would like to inquire about
} } a good negative scanner and/or a better digital imaging system. Can
} } anyone give me some advise? I woud appreciate any help I can get.
} } Thanks, Gretl Nunnemacher
} }
} }
} } -----------------------------------------
} } CONFIDENTIALITY NOTICE: This email communication and any attachments
} } may contain confidential and privileged information for the use of
} } the designated recipients named above. If you are not the intended
} } recipient, you are hereby notified that you have received this
} } communication in error and that any review, disclosure,
} } dissemination, distribution or copying of it or its contents is
} } prohibited. If you have received this communication in error, please
} } reply to the sender immediately or by telephone at (413) 794-0000 and
} } destroy all copies of this communication and any attachments. For
} } further information regarding Baystate Health System's privacy
} } policy, please visit our Internet web site at
} } http://www.baystatehealth.com.
} }
} }
}
}


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 16:13:20 2005



From: slc6-at-lehigh.edu (by way of MicroscopyListserver)
Date: Wed, 9 Feb 2005 16:14:49 -0600
Subject: [Microscopy] viaWWW: SPM Course at Lehigh U

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (slc6-at-lehigh.edu) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, February 9, 2005 at 14:55:19
---------------------------------------------------------------------------

Email: slc6-at-lehigh.edu
Name: Sharon Coe

Organization: Lehigh University

Title-Subject: [Microscopy] [Filtered] MListserver: SPM Course at Lehigh U

Question: SCANNING PROBE MICROSCOPY: FROM FUNDAMENTALS TO ADVANCED
APPLICATIONS
June 6-9, 2005 Lehigh University, Bethlehem, PA
www.lehigh.edu/microscopy

This course introduces the concepts, instrumentation, and
applications of the rapidly expanding field of scanning probe
microscopy (SPM) for beginners and advanced users. The course will
include the practical and theoretical basis of AFM and STM operation.
Instruction and hands-on labs will span the range of basic operation
to advanced techniques, even presenting some of the newest 'custom
designed' approaches that will be the future of the field.
Participants will take away the necessary background to utilize the
full potential of SPM for semiconductor, MEMS, data storage, and
biological applications.

This course is presented by instructors with distinguished
records in inventing advanced SPM probes, producing SPM based
textbooks and instructional materials, and leading the implementation
of SPM in electronics, nanotechnology, and bio-related fields.

Prof. Dawn Bonnell (University of Pennsylvania),
Dr. Joe Griffith (Lucent Technologies, Bell Labs - Retired),
Prof. Richard Vinci (Lehigh University),
Prof. Brian Huey (University of Connecticut) and
Dr. Sergei Kalinin (Oak Ridge National Lab)

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 9 23:21:02 2005



From: Rosemary White :      Rosemary.White-at-csiro.au
Date: Thu, 10 Feb 2005 16:22:24 +1100
Subject: [Microscopy] Re: LM - GFP in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Shea,

On a confocal, you can collect chlorophyll autofluorescence above about 650
nm and if you collect GFP in a narrow band in the green, you should be able
to detect the "pure" green from GFP separately from any chlorophyll
autofluorescence. You could also subtract the red from green, or use
whatever dye separation package is available on the microscope. While
chlorophyll is not supposed to autofluoresce in the green, we routinely see
some fluorescence there. If your chloroplasts are "suffering" you'll get
more autofluroescence in the green and yellow. Treating the tissue with
DCMU should reduce chlorophyll fluorescence, it's a herbicide that attacks
one of the quinones (can't remember which) in the photosynthesis electron
transport pathway. Not sure if it has other effects in the cell. In
contrast (if I remember correctly), PCMBS will enhance chlorophyll
fluorescence.

If you have narrow-band emission filters on a regular fluorescence
microscope, you could collect chlorophyll autofluorescence using a Texas Red
filter, and GFP with a narrow-band green emission filter, and overlay them.
If GFP is there, you should see it.

good luck,
cheers,
Rsoemary

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia

} From: "Miller, Shea" {MILLERS-at-agr.gc.ca}
} Date: Wed, 9 Feb 2005 08:12:06 -0500
} To: {microscopy-at-microscopy.com}
} Subject: [Microscopy] LM - GFP in plants
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Hi all:
}
} I have a user who is trying to look at GFP expression in plant leaves. We
} are having a hard time with the fluorescence from the chlorophyll masking
} everything else. Is there some way to quench the chlorophyll, without killing
} the GFP?
}
}
}
} thanks in advance
}
} shea
}
}
} Dr. S. Shea Miller
} Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
} Telephone/Téléphone: 613-759-1760
} Facsimile/Télécopieur: 613-759-1701
} Rm 2068 K.W. Neatby Bldg
} 960 Carling Ave.
} Central Experimental Farm
} Ottawa, ON
} K1A 0C6
} millers-at-agr.gc.ca
}
} Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 10 01:45:20 2005



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.ac.be
Date: Thu, 10 Feb 2005 08:46:02 +0100
Subject: [Microscopy] RE: Re: LM - GFP in plants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Shea,

If available of course, spectral unmixing, as possible with the Zeiss
META detector on a confocal microscope, you should be perfectly able to
split the GFP from autofluorescence! Otherwise, as stated before,
subtraction of autofluorescence in another channel (650nm) from the by a
narrow GFP-filter obtained image might already help you much further!

Sven


On Feb 9, 2005, at 7:12 AM, Miller, Shea wrote:

}
}
} ----------------------------------------------------------------------
} -
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------
} --------
}
} Hi all:
}
} I have a user who is trying to look at GFP expression in plant
} leaves. We are having a hard time with the fluorescence from the
} chlorophyll masking everything else. Is there some way to quench the

} chlorophyll, without killing the GFP?
}
}
}
} thanks in advance
}
} shea
}
}
} Dr. S. Shea Miller
} Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
} Telephone/Téléphone: 613-759-1760
} Facsimile/Télécopieur: 613-759-1701
} Rm 2068 K.W. Neatby Bldg
} 960 Carling Ave.
} Central Experimental Farm
} Ottawa, ON
} K1A 0C6
} millers-at-agr.gc.ca
}
} Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire
} Canada
}
}
}
}
R. Howard Berg, Ph.D.
Director, Integrated Microscopy Facility
Danforth Plant Science Center
975 N. Warson Rd.
St. Louis, MO 63132

ph 314-587-1261 fx 314-587-1361 cell 314-378-2409
rhberg-at-danforthcenter.org www.danforthcenter.org
visit this educational resource: http://www.danforthcenter.org/Cells/






From MicroscopyL-request-at-ns.microscopy.com Thu Feb 10 13:50:28 2005



From: Lett, Jaclynn :      LettJ-at-ent.wustl.edu
Date: Thu, 10 Feb 2005 13:51:04 -0600
Subject: [Microscopy] TEM: JEOL 100S

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are planning to sell a 27-year old JEOL 100S TEM (with water chiller
and vacuum pumps; uses 3x4-inch plate film). If any of you have
purchased or sold an old scope recently, I would like to know the price
paid.

Thank you so much for your help,

Jaclynn Lett
Senior Research Technician
ENT Research Center
Washington University School of Medicine
Department of Otolaryngology
660 S. Euclid Avenue, Campus Box 8115
St. Louis, MO 63110

lettj-at-ent.wustl.edu

Voice: 314-747-7257
Fax: 314-747-7230




From MicroscopyL-request-at-ns.microscopy.com Fri Feb 11 03:15:41 2005



From: Ursula Potter :      U.J.Potter-at-bath.ac.uk
Date: Fri, 11 Feb 2005 09:16:56 +0000
Subject: [Microscopy] TEM of phage in section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

Has anyone ever looked at bacteriophage in sectioned material. I have been
asked if this is possible and to embed and section a bacterium with phage
either on the outside or inside. Apart from immunocytochemical labelling
and negative staining of extracted phage I am pretty sure that its not
possible to distinguish phage in sections. However, I would be grateful for
any comments on the problem.

Thanks & Regards
Ursula
-----------------

Ursula J. Potter
Centre for Electron Optical Studies
The University of Bath
Claverton Down
Bath BA2 7AY
UK
Tel: 01225 385651
E-mail: U.J.Potter-at-bath.ac.uk


From MicroscopyL-request-at-ns.microscopy.com Fri Feb 11 05:10:29 2005



From: Reinhard Rachel :      reinhard.rachel-at-biologie.uni-regensburg.de
Date: Fri, 11 Feb 2005 12:11:24 +0100
Subject: [Microscopy] TEM of phage in sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just found a very recent article, covering this topic. I'm sure there

are earlier papers as well.

Kind regards,

Reinhard Rachel

Sandra Chibani-Chennoufi et al (2004) J. Bacteriol. 2004 November;
186(21): 7069 7083.


-------------------------------
PD Dr.Reinhard Rachel
Universität Regensburg
Lehrstuhl für Mikrobiologie
Universitaetsstr. 31
D-93053 Regensburg
tel.: +49 941 943 4534
fax.: +49 941 943 1824




From MicroscopyL-request-at-ns.microscopy.com Fri Feb 11 06:28:44 2005



From: Philip Koeck :      Philip.Koeck-at-biosci.ki.se
Date: Fri, 11 Feb 2005 14:46:14 +0100
Subject: [Microscopy] TEM: problems with NMR

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ursula,

I have and you can see some detail (not as much as in negative stain).
You can make out tail and head quite clearly. The only thing is you
need to look at quite a few cells to get the perfect picture with the
phage in section attached to the surface of the bacterium. I think I
could even see the difference between phage containing DNA and empty
ones still attached. You can of course see the forming phage inside the
bug, as well.

If you want, I can see if I can rustle up a couple of pictures - off
the newsgroup of course.


Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk



----- Original Message -----
} From: Ursula Potter {U.J.Potter-at-bath.ac.uk}

Hi,

Does anybody know of any problems with an NMR unit
about 6 meters away from a 200 kV FEGTEM used for
imaging up to about 0.3 nm resolution?

Yours,

Philip




From MicroscopyL-request-at-ns.microscopy.com Fri Feb 11 10:44:23 2005



From: Christopher Gilpin :      christopher.gilpin-at-utsouthwestern.edu
Date: Fri, 11 Feb 2005 11:05:24 -0600
Subject: [Microscopy] TEM: problems with NMR

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Philip

I don't know which TEM and NMR you mean, but I do know of some problems
with a Hitachi S2300 SEM. The NMR was a 15 year old JEOL with a
superconducting magnet (LN2 and He cooled) and it was between 5-10
metres from the SEM.

The operator of the SEM reckoned that he had to adjust his stigmators
quite a bit more but could still achieve reasonable results because the
field didn't change much. Of course the 2300 is thermionic tungsten SEM
and not very high resolution so you might expect more problems with a
field emission gun TEM maybe at lower voltages.

The NMR manufacturer should have information about the intensity and
shape of the magnet's field and you should be able to compare that with
the sensitivity of magnetic field that affects the TEM. A site survey
should be carried out if one or other of the instruments is about to be
installed anyway.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk



----- Original Message -----
} From: Philip Koeck {Philip.Koeck-at-biosci.ki.se}

Philip,
We have an NMR close to our 200Kv FEG. Not as close as yours though! - about
50 feet diagonally up 2 floors.
I asked many questions prior to our installation (the NMR was there first).
The result of the questions is that the field near the microscope is static
DC around 5m Gauss with fluctuations well below the requirements of the
microscope. The only time when there could be a problem is when the magnets
are run up and down. Our NMR is a twin 14.4T system.

We had an additional problem however. The external doors in our building
are made from metal for fire precautions. Each time a door is opened the
"static" DC field changes! This was identified at site survey time and was
amusingly confirmed by me running up and down stairs opening doors whilst
recording field changes at the proposed microscope location. We decided to
install a field cancelling system and this takes care of the problem.

Hope this helps

Chris


Christopher J. Gilpin Ph.D.
Assistant Professor
Director, Molecular and Cellular Imaging Facility
K1.A04
Department of Cell Biology
University of Texas Southwestern Medical Center
5323 Harry Hines Boulevard
Dallas, Texas 75390-9039
+1 214 648 2827 Phone
+1 214 648 6408 Fax
christopher.gilpin-at-utsouthwestern.edu


-----Original Message-----
} From: Philip Koeck [mailto:Philip.Koeck-at-biosci.ki.se]
Sent: Friday, February 11, 2005 7:46 AM
To: microscopy-at-msa.microscopy.com

Hi,

Does anybody know of any problems with an NMR unit about 6 meters away from
a 200 kV FEGTEM used for imaging up to about 0.3 nm resolution?

Yours,

Philip





From MicroscopyL-request-at-ns.microscopy.com Fri Feb 11 14:21:21 2005



From: Eric Anderson :      andersone1-at-southernct.edu
Date: Fri, 11 Feb 2005 15:21:45 -0500
Subject: [Microscopy] Philips EM400 Leak Tip?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings all,

We very suddenly developed a significant vacuum leak somewhere in the
viewing chamber or plate camera of our EM400, as PV3 won't come down any
lower than to about 30 microamps. Before I start checking every last
joint, are there any very typical seals or valves that fail in this
portion of the scope?

Thanks so much for any tips!
Best regards,
~Eric

Eric Anderson
Southern Connecticut State University
Physics Department - JE108B
501 Crescent Street
New Haven, CT 06515
203-392-6468
fax 203-392-6466




From MicroscopyL-request-at-ns.microscopy.com Sat Feb 12 03:35:07 2005



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Sat, 12 Feb 2005 09:35:31 +0000
Subject: [Microscopy] Re: Philips EM400 Leak Tip?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

The large O-ring that seals the lid. You need to lift it out and
clean the lid face, O-ring itself and the seating.

Next try the drive mechanism which moves the plates in and out. If
you swing out the rear unit, the drive mechanism is fairly easy to
get at. If I remember correctly, 3 bolts hold it in place. Remove
these and the whole mechnism comes out (you'll have to power down the
microscope and depressurise the pneumatics so you can disconnect the
pneumatic hoses from the piston).

The drive rod often dries out or gets dirt on it or, occassionally,
scratched. You'll need to check the sliding seal between the
pneumatic piston and the camera chamber.

The pointer seal is also worth checking.

These three are my first choice because they're either frequently
broken or they're sliding seals. If you have a CCD camera in the 35
mm port, I think I'd go for that first. Remove it completely and
replace the original blaning plates.
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)


From MicroscopyL-request-at-ns.microscopy.com Sat Feb 12 14:41:57 2005



From: staylor-at-fit.edu (by way of Ask-A-Microscopist)
Date: Sun, 13 Feb 2005 07:43:25 +1100
Subject: [Microscopy] AskAMicroscopist: LKB 7800 glass knifemaker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (staylor-at-fit.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Micro-Form.html
on Friday, February 11, 2005 at 09:33:25
---------------------------------------------------------------------------

Email: staylor-at-fit.edu
Name: Scott Taylor

School: Fl. Inst. of Technology

State: FL

Zip: 32901

Question: Does anyone know where I can purchase replacement scoring
wheels for the LKB 7800 glass knifemaker?

Thank you!

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Feb 12 21:26:15 2005



From: jdatkins-at-ualr.edu (by way of Ask-A-Microscopist)
Date: Sun, 13 Feb 2005 14:27:42 +1100
Subject: [Microscopy] AskAMicroscopist: cyanobacteria-anabaena cell

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jdatkins-at-ualr.edu) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Saturday, February 12, 2005 at 15:46:24
---------------------------------------------------------------------------

Email: jdatkins-at-ualr.edu
Name: Jennifer Wells

Organization: UALR(University of Arkansas at Little Rock

Education: Undergraduate College

Location: Little Rock, Arkansas

Question: How do I measure the photosynthetic and heterocysts of the
cyanobacteria-anabaena cell with the base pointer of the microscope?
I know that the width of the base is 15mm. I also know that most
photosynthetic cells are 5 micrometers and most heterocysts cells are
larger, about 9 to 10 micrometers. How would I get a filament value
for how many of each would fit inside the 15mm base?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sun Feb 13 10:53:13 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Sun, 13 Feb 2005 11:53:36 -0500
Subject: [Microscopy] Re: TEM of phage in section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ursula,
Check out the references below for cyanobacteria infected with phage.
They are from back in the late 60's but to my knowledge the quality has not
been surpassed since. One of the authors later became my husband so I knew
the work quite well. We have used this fixation (PAF-Acrolein) periodically
through the years with excellent results each time.

Safferman, R.S., Morris, M.E., Sherman, L.A. and Haselkorn, R. 1969.
Serological and electron microscopic characterization of a new group of
blue-green alga viruses (LPP-2). Virology 39:755-780.

Sherman, L.A. and Haselkorn, R. 1970. LPP-1 infection of the blue-green
alga Plectonema boryanum. I. Electron Microscopy J. Virol. 6:820-833.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 2/11/05 4:16 AM, "Ursula Potter" {U.J.Potter-at-bath.ac.uk} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Dear all,
}
} Has anyone ever looked at bacteriophage in sectioned material. I have been
} asked if this is possible and to embed and section a bacterium with phage
} either on the outside or inside. Apart from immunocytochemical labelling
} and negative staining of extracted phage I am pretty sure that its not
} possible to distinguish phage in sections. However, I would be grateful for
} any comments on the problem.
}
} Thanks & Regards
} Ursula
} -----------------
}
} Ursula J. Potter
} Centre for Electron Optical Studies
} The University of Bath
} Claverton Down
} Bath BA2 7AY
} UK
} Tel: 01225 385651
} E-mail: U.J.Potter-at-bath.ac.uk
}




From MicroscopyL-request-at-ns.microscopy.com Mon Feb 14 04:10:12 2005



From: Massimo :      andromeda_tm-at-libero.it
Date: Mon, 14 Feb 2005 11:11:25 +0100
Subject: [Microscopy] Microphotos and reticule eyepiece...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Torino 14 February 2005
(ITALY)

Hi everyone,

I was wondering if anyone is familiar with a Lomo MFN-11 head and its Zenit 122 camera, placed on his microscope.
I want to do some photos and I’d like to talk about the adjustment of the focus on the film plane through the eyepiece reticule.
I do not understand how I can focus the reticule. Have I to keep the alone eyepiece and, seeing through it, to turn the frontal lens up to I see the reticule on focus, then place it in the eyepiece place.
But in such way I do not understand what’s the matter with focusing the image on the film plane.
Thank you in advance for your assistance.
Best Regards,

Massimo




____________________________________________________________
Navighi a 2 MEGA e i primi 3 mesi sono GRATIS.
Scegli Libero Adsl Flat senza limiti su http://www.libero.it





From MicroscopyL-request-at-ns.microscopy.com Mon Feb 14 11:02:32 2005



From: carl :      cboswell-at-email.arizona.edu
Date: Mon, 14 Feb 2005 10:04:18 -0700
Subject: [Microscopy] axioplan parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,
I'm looking for the Fluorescent attachment, whole or in part, for an older
Zeiss Axioplan. Pictures of the the scope and the rear attachemnt site can
be found at: http://www.mcb.arizona.edu/ipc/cb/axioplan.htm. Fluorescent
cubes/slider would also be good.

Any information would be greatly appreciated.

Thanks very much,
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-626-8469
FAX 520-621-3709



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 14 14:10:09 2005



From: engstler-at-lmu.de (by way of MicroscopyListserver)
Date: Tue, 15 Feb 2005 07:11:22 +1100
Subject: [Microscopy] viaWWW: best way to remove azide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (engstler-at-lmu.de) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Monday, February 14, 2005 at 10:40:09
---------------------------------------------------------------------------

Email: engstler-at-lmu.de
Name: Markus Engstler

Organization: University of Munich

Title-Subject: [Microscopy] [Filtered] MListserver: azide

Question: Hi - I 'd like to do some work on fluid phase endocytosis
of (BSA)gold-conjugates (5nm). Most preparations contain azide, which
will kill my cells. What is the best way to remove azide from the
gold-conjugate without diluting/harming the preparation. Any advice
is appreciated, Markus

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 14 17:18:38 2005



From: Jerry Kudenov :      afjdk-at-uaa.alaska.edu
Date: Mon, 14 Feb 2005 14:19:51 -0900
Subject: [Microscopy] Need LM Recipe for Muller's Fluid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

A graduate student here is looking for a recipe for Mullers fluid. I have it
someplace but am unable to lay my hands on it. It is a hardening solution
based on potassium dichromate, sodium sulfate and water. What are the
suggested/recommended proportions. Thank you in advance!

Sincerely,

Jerry

-------------------------------------
Jerry D. Kudenov
Dept Biological Sciences
Univ. Alaska Anchorage
3211 Providence Dr
Anchorage, AK 99508

Office: 907-786-1769
Fax???? 907-786-4607
Email: afjdk-at-uaa.alaska.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 15 08:20:29 2005



From: Ruth Kramer :      ruthie-at-mtu.edu
Date: Tue, 15 Feb 2005 09:20:40 -0500
Subject: [Microscopy] Fwd: RE: weekend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Hi David,

Hope the rest of your weekend went well. Betsy
never made it up because she had the flu, but
here is the message she sent me. It won't hurt to
connect with her.

Hope to see you on March 19th at Flo's for Byrd's 60th birthday. Take care.

Ruthie

} Subject: [Microscopy] RE: weekend
} Date: Mon, 14 Feb 2005 14:38:26 -0600
} Thread-Topic: weekend
} Thread-Index: AcUSviUD5CIoEvaRTiCR2nIXMgdjFgAFug8w
} From: "Schultz, Elizabeth \(STP\)" {elizabeth.schultz-at-guidant.com}
} To: "Ruth Kramer" {ruthie-at-mtu.edu}
} X-OriginalArrivalTime: 14 Feb 2005 20:38:27.0052
} (UTC) FILETIME=[22C3D2C0:01C512D5]
} X-PMX-Version: 4.7.0.111621, Antispam-Engine:
} 2.0.2.0, Antispam-Data: 2005.2.14.12
} X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%,
} Report='__C230066_P5 0, __CT 0, __CTE 0,
} __CTYPE_CHARSET_QUOTED 0, __CT_TEXT_PLAIN 0,
} __HAS_MSGID 0, __HIGHBITS 0, __IMS_MSGID 0,
} __MIME_VERSION 0, __SANE_MSGID 0'
}
} Ruthie,
}
} Glad to hear your trip well well. I feel bad
} missing David-feel free to give him my email
} address in case he has any concerns. He can
} also email me his resume. Whatever works!
}
} B
}
} -----Original Message-----
} From: Ruth Kramer [mailto:ruthie-at-mtu.edu]
} Sent: Monday, February 14, 2005 11:52 AM
} To: Schultz, Elizabeth (STP)
} Subject: [Microscopy] Re: weekend
}
}
} Hi,
}
} Yes, we are still planning on coming. I haven't
} made a motel reservation yet, but will probably
} do that today or tomorrow. We hope to stay at the
} one motel in Cambridge, and it is just off 35 -
} easy to find. I'm sure your cousins will be
} delighted to see you, also!
}
} Sorry to hear you weren't feeling well. I was
} afraid you didn't come because I was gone. If
} there is any consolation, the warm weather made
} this one of the worst seasons for statues we have
} ever had.
}
} I'm so glad I went to Aunt Mary's funeral. About
} 22 out of the remaining 43 cousins were there. I
} put on 1300 miles between Thursday and Sunday and
} was exhausted by the time I got home yesterday.
} Wish your Dad could have made it.
}
} I'll get the details of the weekend to you later
} this week. Angela's family will be staying at the
} same motel so I think it makes sense to do
} everything there. We will probably just go out
} for a meal rather than anyone trying to fix &
} bring. there is a pool and stuff, so we can just
} relax there.
}
} Love,
} R.
}
}
} --
} §§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§
}
} Ruth I. Schultz Kramer
} Scientist, Dept. of Materials Science and Engineering
} Michigan Technological University
} Houghton, MI 49931
} 906-487-3375


--
§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§

Ruth I. Schultz Kramer
Scientist, Dept. of Materials Science and Engineering
Michigan Technological University
Houghton, MI 49931
906-487-3375



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 15 11:10:09 2005



From: psconnel-at-sas.upenn.edu
Date: Tue, 15 Feb 2005 12:10:27 -0500
Subject: [Microscopy] Re: LM Recipe for Muller's Fluid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Quoting Jerry Kudenov {afjdk-at-uaa.alaska.edu} :
} A graduate student here is looking for a recipe for Mullers fluid. I have it
} someplace but am unable to lay my hands on it. It is a hardening solution
} based on potassium dichromate, sodium sulfate and water. What are the
} suggested/recommended proportions. Thank you in advance!
}
} Sincerely,

} Jerry D. Kudenov
} Dept Biological Sciences
} Univ. Alaska Anchorage
} 3211 Providence Dr
} Anchorage, AK 99508
======================
Jerry,

Muller's Fluid is as follows:
Potassium bichromate 2.5 Gm.
Sodium sulfate crystals 1.0 Gm.
Distilled water 100 cc.

The reference is in Histopathologic Technic and Practical Histochemistry
by R. D. Lillie 1954 on page 332. The coppyright is The Blakiston Company, Inc.
but printed by the Country Life Press Corp., Garden City, NY

Muller's Fluid was used as a Fixative and a Mordant by Pal and by Wolters for
Myelin for 2 - 3 weeks each step.

I knew this old book would have a use!

Pat Connelly
Dept. of Biology
Univ. of Pennsylvania
Philadelphia, PA 19104-6018
psconnel-at-sas.upenn.edu



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 15 13:50:50 2005



From: amal1966_2-at-yahoo.com (by way of Ask-A-Microscopist)
Date: Wed, 16 Feb 2005 07:07:06 +1100
Subject: [Microscopy] AskAMicroscopist: Bone samles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hey

Flo didn't invite me.

Am I on the outer, or what?


rtch


Date sent: Tue, 15 Feb 2005 09:20:40 -0500
To: Microscopy-at-microscopy.com
} From: Ruth Kramer {ruthie-at-mtu.edu}

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (amal1966_2-at-yahoo.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, February 15, 2005 at 02:36:45
---------------------------------------------------------------------------

Email: amal1966_2-at-yahoo.com
Name: Amal El Shikh

Education: Graduate College

Location: Saudi Arabia

Question: How I can prepare Bone samles For Electron microscopy

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 15 15:05:30 2005



From: Ken Converse :      kenconverse-at-qualityimages.biz
Date: Tue, 15 Feb 2005 16:04:45 -0500
Subject: [Microscopy] Stigmator, aperture, and lens adjustment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ritchie,
Somehow I didn't make the shift to the new system, but now I'm back on
(thanks, Nestor). I had held this message from you and checked the January
archives and didn't see the reply you were looking for, so here's the simple
explanation of what they're trying to do:

When you flip the objective lens polarity back and forth, the images flips
about 180 degrees (or perhaps something less), but that point of rotation
isn't necessarily in the center of the CRT. The idea is to try and get some
identifiable point on your sample moved to the center of rotation (i.e.
about half way between the 2 places that it shows up when you flip the
switch).

Once your identifiable point no longer moves (or moves very far), you then
use the adjustment handles for the condenser lens to move that point to the
center of the CRT. Now, if you flip the lens polarity, the image should
rotate around the center of the CRT. It is important to use the CLEAR OL
button (I always do both lenses at the same time, and hold them down for not
less than 1 second, 2 may be better, but consistency is also important) to
find the true center of rotation.

I think one of the reasons their directions are so confusing is because most
of the time you are going to need to make corrections on 2 axes to get your
point to the point of rotation and they don’t explain that that is what they
are doing.

Hope this helps.

Ken Converse

owner 
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
} From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz]
Sent: Wednesday, December 29, 2004 6:55 PM
To: microscopy-at-microscopy.com


Thanks, Randy and shAf, for your contributions re stigmator and aperture
adjustment.

I've never been able to make much sense of the JEOL (840) instructions for
condenser
lens alignment. It seems they take me into a loop from which there is no
escape other
than giving up (yet again) in frustration.

Do you guys, or does anyone, have either a lucid step-by-step procedure for
this, or an
explanation of what is aiming to be achieved that I can translate into
specifics for the
840?

I use the thing only as a microprobe, so the spot tightness isn't a real
issue, but it would
be nice to be able to do the sort of imaging that I know the 840 is capable
of, and I
suspect that beam current stability is improved by good alignment.

Happy New Year

rtch



--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand






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From MicroscopyL-request-at-ns.microscopy.com Tue Feb 15 15:21:22 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 16 Feb 2005 10:21:57 +1300
Subject: [Microscopy] apology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I apologise to you, Ruth, and to the list, for being so mean-spirited and grumpy and
reforwarding the personal email which Ruth accidentally sent to the list.

I haven't yet made that simple mistake, but maybe I will in the future.

sorry

cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 15 16:34:33 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Tue, 15 Feb 2005 14:34:26 -0800 (PST)
Subject: [Microscopy] Re: Re: Fwd: RE: weekend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i didn't get an invite either.
now i am depressed.
--- Ritchie Sims {r.sims-at-auckland.ac.nz} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
}
} Hey
}
} Flo didn't invite me.
}
} Am I on the outer, or what?
}
}
} rtch
}
}
} Date sent: Tue, 15 Feb 2005 09:20:40 -0500
} To: Microscopy-at-microscopy.com
} } From: Ruth Kramer {ruthie-at-mtu.edu}
} Subject: [Microscopy] Fwd: RE: weekend
}
} }
} }
} }
}
----------------------------------------------------------------------
} } -------- The Microscopy ListServer -- Sponsor:
} The Microscopy Society
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------
} } ---------
} }
} } } Hi David,
} }
} } Hope the rest of your weekend went well. Betsy
} } never made it up because she had the flu, but
} } here is the message she sent me. It won't hurt to
} } connect with her.
} }
} } Hope to see you on March 19th at Flo's for Byrd's
} 60th birthday. Take
} } care.
} }
} } Ruthie
} }
} } } Subject: [Microscopy] RE: weekend
} } } Date: Mon, 14 Feb 2005 14:38:26 -0600
} } } Thread-Topic: weekend
} } } Thread-Index:
} AcUSviUD5CIoEvaRTiCR2nIXMgdjFgAFug8w
} } } From: "Schultz, Elizabeth \(STP\)"
} {elizabeth.schultz-at-guidant.com}
} } } To: "Ruth Kramer" {ruthie-at-mtu.edu}
} X-OriginalArrivalTime: 14 Feb 2005
} } } 20:38:27.0052 (UTC) FILETIME=[22C3D2C0:01C512D5]
} X-PMX-Version:
} } } 4.7.0.111621, Antispam-Engine: 2.0.2.0,
} Antispam-Data: 2005.2.14.12
} } } X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%,
} Report='__C230066_P5 0,
} } } __CT 0, __CTE 0, __CTYPE_CHARSET_QUOTED 0,
} __CT_TEXT_PLAIN 0,
} } } __HAS_MSGID 0, __HIGHBITS 0, __IMS_MSGID 0,
} __MIME_VERSION 0,
} } } __SANE_MSGID 0'
} } }
} } } Ruthie,
} } }
} } } Glad to hear your trip well well. I feel bad
} } } missing David-feel free to give him my email
} } } address in case he has any concerns. He can
} } } also email me his resume. Whatever works!
} } }
} } } B
} } }
} } } -----Original Message-----
} } } From: Ruth Kramer [mailto:ruthie-at-mtu.edu]
} } } Sent: Monday, February 14, 2005 11:52 AM
} } } To: Schultz, Elizabeth (STP)
} } } Subject: [Microscopy] Re: weekend
} } }
} } }
} } } Hi,
} } }
} } } Yes, we are still planning on coming. I haven't
} } } made a motel reservation yet, but will probably
} } } do that today or tomorrow. We hope to stay at the
} } } one motel in Cambridge, and it is just off 35 -
} } } easy to find. I'm sure your cousins will be
} } } delighted to see you, also!
} } }
} } } Sorry to hear you weren't feeling well. I was
} } } afraid you didn't come because I was gone. If
} } } there is any consolation, the warm weather made
} } } this one of the worst seasons for statues we have
} } } ever had.
} } }
} } } I'm so glad I went to Aunt Mary's funeral. About
} } } 22 out of the remaining 43 cousins were there. I
} } } put on 1300 miles between Thursday and Sunday and
} } } was exhausted by the time I got home yesterday.
} } } Wish your Dad could have made it.
} } }
} } } I'll get the details of the weekend to you later
} } } this week. Angela's family will be staying at the
} } } same motel so I think it makes sense to do
} } } everything there. We will probably just go out
} } } for a meal rather than anyone trying to fix &
} } } bring. there is a pool and stuff, so we can just
} } } relax there.
} } }
} } } Love,
} } } R.
} } }
} } }
} } } --
} }
}
} §§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§
} } }
} } } Ruth I. Schultz Kramer
} } } Scientist, Dept. of Materials Science and
} Engineering
} } } Michigan Technological University
} } } Houghton, MI 49931
} } } 906-487-3375
} }
} }
} } --
} }
}
§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§§
} }
} } Ruth I. Schultz Kramer
} } Scientist, Dept. of Materials Science and
} Engineering
} } Michigan Technological University
} } Houghton, MI 49931
} } 906-487-3375
} }
} }
}
}
}
}
}




__________________________________
Do you Yahoo!?
Take Yahoo! Mail with you! Get it on your mobile phone.
http://mobile.yahoo.com/maildemo


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 15 16:43:11 2005



From: BETTY FABER :      bfaber-at-lsc.org
Date: Tue, 15 Feb 2005 17:44:04 -0500
Subject: [Microscopy] Microscope suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are expanding and improving our exhibit floors at Liberty Science
Center. We do have some experience using microscopes on the exhibit
floors (for use by school children and other members of the general
public) of a science center, but are quite open to suggestions and
recommendations.


We have used Wentzscopes, but would like to consider other methods.
What other recommendations does the list have for viewing objects the
size of red blood cells?

We have used student grade dissection scopes to view opaque objects.
These tend to have gears stripped and worse within 6 months. Is there
an industrial grade of dissection scope that is bullet proof?

We are leery of digital scopes. Should we be?

What we really want is microscopes that are very durable and easy to
use. Microscopes that allow the viewer to see the kinds of images that
inspire them to think seriously about the sciences.

I really appreciate your thoughts and time.


Betty Faber, Ph.D.
Leader Program Development
Liberty Science Center
201-451-0006X260
Bfaber-at-lsc.org




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 15 17:16:23 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 15 Feb 2005 15:17:50 -0800
Subject: [Microscopy] Re: viaWWW: best way to remove azide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

dialysis

At 12:11 PM 2/14/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Tue Feb 15 17:51:24 2005



From: John Brealey :      john.brealey-at-imvs.sa.gov.au
Date: Wed, 16 Feb 2005 10:20:46 +1030
Subject: [Microscopy] Transport of a TEM - advice needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have a few questions regarding the transport of a Hitachi H-600.
I am currently seeking to have the TEM dismantled and moved from Melbourne
to Adelaide (800km) by a removalist company specialising in sensitive
freight.
Should the HV cable be removed from the gun and HV tank or can it be left
attached at one or both ends?
If it can be left attached at one end, which end is best to leave attached?
If the HV cable is removed from the HV tank, should the tank be drained of
oil or left full and covered by the blanking plate?
How can the HV cable best be wrapped to minimise damage?
How long would it take to dismantle the TEM and what is the minimum number
of component parts that the TEM can be broken down to? For example, is left
console, right console, camera console, column, HV cable, HV tank and
electronics box a reasonable breakdown of components (this is excluding
rotary pumps, compressor and desiccator)?

Any advice or personal experiences on this issue welcome.

Thanks,

____________________________
John Brealey
Medical Scientist
Electron Microscopy Unit
The Queen Elizabeth Hospital
IMVS - TQEH Pathology
Woodville, 5011
Adelaide
South Australia
(08) 82226612



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 02:42:49 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 16 Feb 2005 00:44:54 -0800
Subject: [Microscopy] Re: Microscope suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Betty
You may check Russian microscopes made by LOMO. I did not see their latest
models, but those we used in the school were bullet/student proof,
really... not only bullets, they perhaps will survive in direct tank
attack... I think, there is some company in US who distribute LOMO
products overseas. You may check google. Microscopes, by the way, made
using classical Zeiss technology, quite good for the price. No any
interest in LOMO rather than nostalgia for LM-2 - 100% quartz-optic
microscope... Sergey

At 05:44 PM 2/15/2005 -0500, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 02:54:27 2005



From: James Chalcroft :      jchalcro-at-neuro.mpg.de
Date: Wed, 16 Feb 2005 09:54:36 +0100
Subject: [Microscopy] Transport of a TEM - advice needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear John,

Sorry that I cannot help you with your Hitachi H-600 removal problem,
nevertheless I would also be most grateful to have help from the list
regarding the dismantling, packing and transport of a Zeiss 902 TEM (for
road transport within Germany). There seem to be a lot of major
components which will have to be separated before it hits the road!
Perhaps someone out there has done just this and could perhaps help us
avoid unnecessary woes during this major exercise.
TIA and best wishes,

Jim



-----Original Message-----
} From: John Brealey [mailto:john.brealey-at-imvs.sa.gov.au]
Sent: Wednesday, February 16, 2005 12:51 AM
To: Listserver

Hi all,

I have a few questions regarding the transport of a Hitachi H-600.
I am currently seeking to have the TEM dismantled and moved from
Melbourne
to Adelaide (800km) by a removalist company specialising in sensitive
freight.
Should the HV cable be removed from the gun and HV tank or can it be
left
attached at one or both ends?
If it can be left attached at one end, which end is best to leave
attached?
If the HV cable is removed from the HV tank, should the tank be drained
of
oil or left full and covered by the blanking plate?
How can the HV cable best be wrapped to minimise damage?
How long would it take to dismantle the TEM and what is the minimum
number
of component parts that the TEM can be broken down to? For example, is
left
console, right console, camera console, column, HV cable, HV tank and
electronics box a reasonable breakdown of components (this is excluding
rotary pumps, compressor and desiccator)?

Any advice or personal experiences on this issue welcome.

Thanks,

____________________________
John Brealey
Medical Scientist
Electron Microscopy Unit
The Queen Elizabeth Hospital
IMVS - TQEH Pathology
Woodville, 5011
Adelaide
South Australia
(08) 82226612





From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 06:14:49 2005



From: =?ISO-8859-1?Q?G=F6ran_Axelsson?= :      axelsson-at-acc.umu.se
Date: Wed, 16 Feb 2005 13:14:51 +0100
Subject: [Microscopy] Transport of a JEOL JEM 100CX TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I changed the subject for the sake of the archives. Old subject was
" Transport of a Zeiss CEM 902 TEM - advice requested"

It isn't the same maker or model but at least it is a description about
how we moved a Jeol 100CX TEM two years ago.
http://www.home.neab.net/gandalf/EM-lab/TEM100CX/index.htm

There are a lot of pictures of the dismantling in the raw picture archive.
A small warning first, the raw pictures are direct from the camera and is on
average 600k so they are slow to load.

We have now dismantled and moved two 100CX TEM:s. The first took
five days to pack and move on five pallets. The second one went a lot
faster and it only took eight hours for two people to put it on three
pallets.
Then it took us six hours to move it to the loading bay and to pack all the
small bits in two cars.
On both moves we left the oil in and only put the cap on the HT tank
and the HT cable was transported in the car.

If you only take it slowly, use a digital camera and takes a huge amount
of pictures (I took 600 pictures on the first microscope) and mark every
cable you should have no problem.
When i put it back together I only needed to look at two pictures to get
it right.

Two good things to have when packing a TEM is plastic film, the type
you use to cover leftovers, to wrap cables and hold things in place.
You use it instead of tape, it doesn't leave glue when taken off and is
easy to put on. In the end we even wrapped the main console from top
to bottom, it keeps dust out of it.
The second thing is aluminium foil to cover any open vacuum connection.

Well, except for that I could only wish you luck.

Göran

James Chalcroft wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 08:01:17 2005



From: monica.iliescu-at-polymtl.ca (by way of MicroscopyListserver)
Date: Thu, 17 Feb 2005 01:02:37 +1100
Subject: [Microscopy] viaWWW: particles in liquid solution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (monica.iliescu-at-polymtl.ca) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, February 15, 2005 at 15:30:22
---------------------------------------------------------------------------

Email: monica.iliescu-at-polymtl.ca
Name: Monica ILIESCU

Organization: Ecole Polytechnique

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello,

I would like to ask is someone of you imaged in ESEM or
SEM-HiVAc,(micro- or nano) particles in liquid solution. If yes, what
the best preparation method is?
The using of a negatively charged mica substrates that catch
(attract) positive charged particles could be a valuable way for High
Vacuum imaging?

Thank you,
Monica

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 09:58:57 2005



From: John Shields :      jpshield-at-uga.edu
Date: Wed, 16 Feb 2005 11:00:01 -0500
Subject: [Microscopy] volunteers for M&M2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,
If you are planning to attend M&M 2005 in Hawaii this summer
and wish to volunteer to assist in various ways
(e.g. "projectionist", booths, helping in any way) then
please contact me at jshields-at-cb.uga.edu with the subject
line "volunteer 2005".
I will contact you with more information, requirements, and
answer any questions I have answers for.
There will *not* be money for travel or room and board.
There will be inexpensive housing and other members have
already provided alternative housing ideas (e.g. hostels).
Students will be able to take advantage of reduced rates at
specific hotels - information is available when you contact
me.
Thanks in advance for your help.
John
} --
} John Shields
} E.M. Lab, 151 Barrow Hall
} University of Georgia
} Athens, GA 30602
} 706-542-4080


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 10:35:14 2005



From: Andrea Brothers :      Andrea.Brothers-at-biovail-btl.com
Date: Wed, 16 Feb 2005 11:35:34 -0500
Subject: [Microscopy] Re: Microscope suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

http://www.lomoplc.com/SF100.htm

Website for LOMO FYI.
AB

Andrea Blake Brothers
Sr. Research Microscopist
andrea.brothers-at-biovail-btl.com

(703) 480-5879 office
(703) 480-5943 fax

Biovail Technologies Ltd.
3701 Concorde Parkway
Chantilly, VA 20151

The information contained in this e-mail message may be privileged
information and is intended only for the use of the individual and/or entity
identified in the address of this message. If the reader of this message is
not the intended recipient, or an employee or agent responsible to deliver
it to the intended recipient, you are hereby requested not to distribute or
copy this communication. If you have received this communication in error,
please notify us immediately by calling us collect at (703) 480-6000, or by
so advising us by return e-mail. In this circumstance, we request that you
delete the original message from your system.


-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Wednesday, February 16, 2005 3:45 AM
To: Microscopy-at-microscopy.com

Betty
You may check Russian microscopes made by LOMO. I did not see their latest
models, but those we used in the school were bullet/student proof,
really... not only bullets, they perhaps will survive in direct tank
attack... I think, there is some company in US who distribute LOMO
products overseas. You may check google. Microscopes, by the way, made
using classical Zeiss technology, quite good for the price. No any
interest in LOMO rather than nostalgia for LM-2 - 100% quartz-optic
microscope... Sergey

At 05:44 PM 2/15/2005 -0500, you wrote:


} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 11:19:33 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 16 Feb 2005 11:19:53 -0600
Subject: [Microscopy] viaWWW: particles in liquid solution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Monica,

One of the easiest ways is to take ordinary cover slips and cover them
with large drops of 1 mg/ml poly-l-lysine. Leave the coverslips in a
humidity chamber (such as a petri dish with a rolled-up soaked Kimwipe
or something similar) for about an hour, then rinse them gently in
distilled water. These coated coverslips will remain useful for a month
or so after drying.

Take some of your particles in liquid and put them on the coated
coverslips and let the particles settle onto the surface, where they
should adhere to the coating. After a half hour or so, gently rinse the
coverslips to remove the excess solution, then proceed with your normal
fixation and/or coating routine. You should be able to view your
particles in high vacuum conditions with little difficulty.

One problem is occasionally that the coverslips are difficult to ground
and can create charging artifacts in the SEM. Usually all you need to
do is be extra careful about mounting the coverslips to your SEM stub.
We usually use carbon adhesive tabs, then metal tape to bridge the top
of the coverslips to the stub by folding the tape over the edges in a
couple of places. Following this, we sputter coat normally, starting
with a light coat, then adding more as necessary until the charging is
under control.

Hope this helps.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu









-----Original Message-----
} From: by way of MicroscopyListserver [mailto:monica.iliescu-at-polymtl.ca]
Sent: Wednesday, February 16, 2005 8:03 AM
To: microscopy-at-ns.microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (monica.iliescu-at-polymtl.ca) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday,
February 15, 2005 at 15:30:22
------------------------------------------------------------------------
---

Email: monica.iliescu-at-polymtl.ca
Name: Monica ILIESCU

Organization: Ecole Polytechnique

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello,

I would like to ask is someone of you imaged in ESEM or
SEM-HiVAc,(micro- or nano) particles in liquid solution. If yes, what
the best preparation method is?
The using of a negatively charged mica substrates that catch
(attract) positive charged particles could be a valuable way for High
Vacuum imaging?

Thank you,
Monica

------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 11:52:02 2005



From: Michael Dunlap :      mrdunlap-at-ucdavis.edu
Date: Wed, 16 Feb 2005 09:52:54 -0800
Subject: [Microscopy] Re: Transport of a TEM - advice needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi John,

I have moved our H-600 several times. The instrument can be broken
down into the individual sections as you described. I did find that
the HV cable did not separate from the gun on our instrument. We had
a 'can' we found on the side of the column and the right console that
held the insulator on the HV cable that was in the HT tank and the
lid of the 'can' sealed the HT to help prevent leaks.

We had to remove the gun and gun jack to get the microscope out the door.

One thing to note, there is a small part (sorry the name escapes me)
that comes loose in the column just above the sample holder. Lift
the gun and condenser lens and it should be right in that space.

Mike

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
===================================================================================
Michael Dunlap
office (530) 752-0284
University of California lab (530) 752-5489
Chemical Engineering & Material Science Fax (530) 752-9554
110A Kemper Hall mrdunlap-at-ucdavis.edu
One Shields Ave.
http://www.chms.ucdavis.edu/
Davis CA, 95616
===================================================================================


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 12:19:04 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 16 Feb 2005 13:19:49 -0500
Subject: [Microscopy] Housing for M&M2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I did a quick check of Expedia and Travelocity. Both have
airfare/hotel packages that offer substantial savings over staying at
the 3 hotels listed in the meeting brochure. I don't know how these
various hotels rate wrt convenience to the convention center, etc.,
but if you've got a tight budget they may make the difference between
going and staying home.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 13:37:11 2005



From: William Stratton :      wgstratton-at-wisc.edu
Date: Wed, 16 Feb 2005 13:36:46 -0600
Subject: [Microscopy] Selective Alumina Etch

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

Can anyone recommend selective alumina etch, specifically one that won't
etch aluminum. I'm looking for a way to clean the alumina from my
aluminum specimens.

Thanks in advance for your help.

William Stratton


-------------------
William G. Stratton
Research Assistant
University of Wisconsin - Madison

1509 University Avenue
Madison, WI 53706
Office: 608-265-6391
Fax: 608-262-8353
wgstratton-at-wisc.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 13:37:31 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Wed, 16 Feb 2005 13:38:25 -0600
Subject: [Microscopy] Re: viaWWW: particles in liquid solution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Monica,

For high-vacuum SEM, we just put a drop of the particle solution on a
glass coverslip or formvar coated TEM grid, blot a little (not to
dryness), cover with something like an inverted petri dish to keep
the sample clean, and allow to air dry.
Your thought of charges and mica should also work fine.

Phil

} Email: monica.iliescu-at-polymtl.ca
} Name: Monica ILIESCU
}
} Organization: Ecole Polytechnique
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: Hello,
}
} I would like to ask is someone of you imaged in ESEM or
} SEM-HiVAc,(micro- or nano) particles in liquid solution. If yes,
} what the best preparation method is?
} The using of a negatively charged mica substrates that catch
} (attract) positive charged particles could be a valuable way for
} High Vacuum imaging?
}
} Thank you,
} Monica
}
} ---------------------------------------------------------------------------

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From MicroscopyL-request-at-ns.microscopy.com Wed Feb 16 18:16:35 2005



From: John Brealey :      john.brealey-at-imvs.sa.gov.au
Date: Thu, 17 Feb 2005 10:45:58 +1030
Subject: [Microscopy] Moving a TEM - thankyou

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi again,

Thanks for the quick replies.
You have provided me with a lot of good advice which I am still digesting.

Regards,

____________________________
John Brealey
Medical Scientist
Electron Microscopy Unit
The Queen Elizabeth Hospital
IMVS - TQEH Pathology
Woodville, 5011
South Australia
(08) 82226612


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 17 06:22:54 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Thu, 17 Feb 2005 08:54:03 -0330
Subject: [Microscopy] x-y-r stage transformations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

hello all :o)

I need to transform x-y coordinates determined from one stage to that of
another. The cartesian origin (0,0) will not be coincident, and rotation
needs to be taken into account. Rather than wrap my brain around the
required analytical geometry gained 30 years ago, I was hoping someone had
already created the spreadsheet. That is, given 2 reference coordinates
(x', y' and x", y") for the original stage, and the new coordinates for the
same on the new stage, transform a list of original coordinates to new
coordinates.

tia & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
{www.micro-investigations.com}



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 17 10:15:09 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 17 Feb 2005 11:17:13 -0800
Subject: [Microscopy] Re: Housing for M&M2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List:

In my recent experience most hotels, airlines and car rental
companies will give you a better deal than Expedia or Travelocity will.
Use Expedia or Travelocity to find a hotel (car, airline), then go to
the hotel's (car agency's, airline's) own website and compare prices..
The added benefit is that if there are billing problems you won't have
to go through a third party. I would be out several hundred dollars if I
had to rely on Expedia to straighten out a billing dispute. Also, if you
use a third party (Expedia, Travelocity) to do the booking your credit
card company may force you to deal with that booking agency to resolve
the dispute. :-(

Geoff

Leona Cohen-Gould wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} I did a quick check of Expedia and Travelocity. Both have
} airfare/hotel packages that offer substantial savings over staying at
} the 3 hotels listed in the meeting brochure. I don't know how these
} various hotels rate wrt convenience to the convention center, etc.,
} but if you've got a tight budget they may make the difference between
} going and staying home.
} Lee


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From MicroscopyL-request-at-ns.microscopy.com Thu Feb 17 13:11:33 2005



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Thu, 17 Feb 2005 13:12:30 -0600
Subject: [Microscopy] Position Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Position Announcement: Visiting Light Microscopist
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign

The Imaging Technology Group (ITG) at the Beckman Institute for Advanced
Science and Technology, UIUC provides facilities for both microscopy and
visualization to campus researchers from a broad spectrum of
disciplines. The ITG Microscopy Suite provides a wide range of
instrumentation, training and user support for electron, scanning probe
and optical microscopy. Optical microscopy capabilities include laser
scanning confocal and multiphoton microscopy, widefield transmitted and
fluorescence microscopy, stereomicroscopy, computer-assisted stereology,
near-field scanning microscopy, reflected/transmitted light
micro-spectroscopy and near IR imaging. More extensive information
about our facilities is available on our web site at
http://www.itg.uiuc.edu.

The ITG is presently conducting a search for a microscopist to serve as
a primary contact for user training, trouble-shooting, and quality
assurance for a Leica SP-2 confocal microscope. In addition to managing
one of the confocal microscopes, the successful applicant will also help
to answer research questions using all of the microscopy techniques
available in the ITG as well as participate in the development of
advanced imaging technologies. A more detailed version of the
announcement is available at:

http://www.itg.uiuc.edu/announcements/lightmicro.htm

This is a 12-month, full-time visiting academic professional position
with university benefits and the possibility of becoming a permanent
position. Salary is commensurate with experience. For full
consideration, applications should be received by March 1st. To apply
please send a letter of interest, CV, and the contact information of
three references to:

Lori Heil
Beckman Institute for Advanced Science and Technology Group
405 North Mathews Avenue
Urbana, IL 61801
Phone: (217) 244-0170
Fax: (217) 244-6219
E-mail: lheil-at-uiuc.edu

Informal questions can be directed to Glenn Fried, ITG Co-director
(gfried-at-itg.uiuc.edu; phone: 333-5493), or myself (contact information
below). Thanks.

Best Regards,
Karl

--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Office: B650J
Phone: 217.244.6292
Fax: 217.244.6219
Mobile: 217.390.1874
www.itg.uiuc.edu



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 17 15:01:59 2005



From: frank.karl-at-degussa.com
Date: Thu, 17 Feb 2005 16:02:39 -0500
Subject: [Microscopy] Chilling Water the TEM Blues

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Hello everyone,

I got a problem that I hope the collective you can help me solve. My
Phillips 420 TEM is suffering from poor circulation. The recirculating
cooling water comes in to the scope and is split, one part to the diffusion
pump and one to the electronics. Both sides have adjustable flow meters
and should have 1.5 L per min rates. Recently the electronic side dropped
to about 400ml per minute and the scope shut off.

Previous experience indicated I needed to flush the cooling water with a
dilute phosphoric acid based detergent. Have done this I can get the DP
side up to 2.5 liters/min if I wanted, but the electronic side stays at 800
ml/min no matter how I adjust the flow meters. The cleaning solution
pulled all the discoloration from sight glass on the flow meter, so I
believe I have the gunk flushed, but I know if I leave it at 800 ml/min
it's only a matter of time before I have to re-flush the cooling lines.

Where else could I have an obstruction and how can I get rid of it?

Any advice and thoughts would be welcome.

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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From MicroscopyL-request-at-ns.microscopy.com Thu Feb 17 17:40:18 2005



From: John V Nailon :      J.Nailon-at-uq.edu.au
Date: Fri, 18 Feb 2005 09:41:14 +1000
Subject: [Microscopy] Re: Chilling Water the TEM Blues

Contents Retrieved from Microscopy Listserver Archives
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G'day Frank,

I've had similar problems on microscopes, usually it is because the
cooling water flows through alloy heat sinks, the alloy corrodes and
occludes the channel through it. On an Electroscan Esem we had, we
reamed out the cooling line through the alloy heat sink and slid copper
tube through, well lubricated with vacuum grease as a heat sink agent.
Hope this helps.

Regards
JVN


frank.karl-at-degussa.com wrote:

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--
John V Nailon
Executive Officer and Operations Manager
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The University of Queensland
St.Lucia QLD 4072 Australia
Phone: 617 3365 4214
Fax: 617 3365 4422
Mobile: 0423 020 680




From MicroscopyL-request-at-ns.microscopy.com Thu Feb 17 18:41:04 2005



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Thu, 17 Feb 2005 19:40:48 -0500
Subject: [Microscopy] Re: Chilling Water the TEM Blues

Contents Retrieved from Microscopy Listserver Archives
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Hi Frank,

I'm not sure if the EM420 used them, but it could be a problem with the
"Watts" regulator for the lenses. It might need to be replaced. We've had
some fail.

For flushing the cooling lines, our service people have been really
enthusiastic about something called "CLR" cleaner (Calcium, Lime,
Rust). If I remember correctly, sulfamic, glycolic, and citric acids are
the active ingredients. I picked it up at our local hardware store
("cleaners" department rather than "plumbing"). It did a good job of
cleaning up the flow in our CM200 and caused no noticeable damage.

You also might try running the cleaner through the system overnight and
throttling back the flow through the DP to increase the pressure through
the lens section.

Cheers,
Henk

At 04:02 PM 2/17/2005, frank.karl-at-degussa.com wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
OSU Campus Electron Optics Facility www.ceof.ohio-state.edu
040 Fontana Labs, 116 W. 19th Ave



From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 10:12:38 2005



From: Gib Ahlstrand :      ahlst007-at-tc.umn.edu
Date: Fri, 18 Feb 2005 10:19:13 -0600
Subject: [Microscopy] Re:Chilling Water the TEM Blues

Contents Retrieved from Microscopy Listserver Archives
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Frank,

Here's a write-up describing what I did some years ago to clear SEM cooling
lines. Just hit upon this idea as I had some lab water pumps lying around.
Maybe this will give you something to try.

But... a cautionary note: our CM-12 TEM has similar split in lines with
adjustable flow valves & floating flowrate indicators as you describe and
I'm not sure if this method would damage those, depending on how they are
built, so take a look at that before trying this. Our SEM cooling lines
didn't have such a fancy control system, water just came in through a fine
wire mesh final filter and into the lines.

Good luck shakin' off them blues! Gib
------
Gib Ahlstrand, Scientist
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-2785 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/

"You can learn a lot by observation - just by lookin'!" - Yogi Berra
-----
Here are my notes, with a few updates, to show you how we cleaned our SEM's
cooling lines some years ago. You can take what might be helpful from this
to apply to your situation there.

Symptom: Intermittent SEM shutdown, due to insufficient cooling water
supply.

BEFORE cleaning, measure the output from your cooler unit, which would be
the input to your machine. Then measure the output from your machine, by
collecting output in a can per minute, measure with graduated beaker. This
will allow you to determine if the cleaning of the cooling lines through
your machine actually achieved any increase in coolant flow-through rate.

Example, on my SEM: Before cleaning, output was 1,050 ml per minute. After
cleaning, rate was 1,800 ml per minute, a 71% increase, and overheating of
SEM problem went away.

1. I used a small lab water circulating pump, a "Little Giant", to pull a
weak 409 detergent cleaner solution through the scope lines, after
disconnecting the scope input lines from the cooling supply. Mix up a gallon
or two of dilute 409 solution, put in large dishpan and stick input hose
from machine into solution. Hook up pump to output end of cooling line,
collect output of pump in another dishpan, so you can inspect it for junk
that might some out of the lines.

2. Another thing we did, was to pump the lines for a few seconds to fill
the lines full of detergent solution, as above, then shut off pump and
removed it from output end of machine. Then at input to machine, we hooked
up a lab vacuum pump which could be run in "reverse" to act as an air
pressurizing device. So then I'd hold my finger over the output end for a
few seconds when this pressurizing pump was turned on, to build up a little
bit of pressure inside the lines, then released my finger to "blast" out any
algae/junk which had built up in the lines. Repeated this a few times, until
that charge of cleaning solution had been expelled. Filled lines and
repeated this a few times. It seemed to help flush the junk out of the
lines. Just don't want to put too much pressure in there or internal seals
could blow out, so just a little bit of finger pressure was all we did.
Could run this set-up in either direction to get a bach & forth effect, may
help to loosen up or free blockages.

3. When lines have been cleaned out, pump some clean water through to flush
out all the 409 detergent.

4. Connect up input lines from your chiller unit, turn on and measure the
hopefully greater output flow from the output of the machine. If satisfied,
hookup the output end of the machine to the return line for your cooling
unit.

Hope this helps! Let me know what results you have with whatever you come up
with.

Gib Ahlstrand

} Hello everyone,
}
} I got a problem that I hope the collective you can help me solve. My
} Phillips 420 TEM is suffering from poor circulation. The recirculating
} cooling water comes in to the scope and is split, one part to the diffusion
} pump and one to the electronics. Both sides have adjustable flow meters
} and should have 1.5 L per min rates. Recently the electronic side dropped
} to about 400ml per minute and the scope shut off.
}
} Previous experience indicated I needed to flush the cooling water with a
} dilute phosphoric acid based detergent. Have done this I can get the DP
} side up to 2.5 liters/min if I wanted, but the electronic side stays at 800
} ml/min no matter how I adjust the flow meters. The cleaning solution
} pulled all the discoloration from sight glass on the flow meter, so I
} believe I have the gunk flushed, but I know if I leave it at 800 ml/min
} it's only a matter of time before I have to re-flush the cooling lines.
}
} Where else could I have an obstruction and how can I get rid of it?
}
} Any advice and thoughts would be welcome.
}
} Frank Karl
} Degussa Corporation
} Akron Technical Center
} 3500 Embassy Parkway
} Suite 100
} Akron, Ohio 44333
} 330-668-2235 Ext. 238



From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 13:43:20 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Fri, 18 Feb 2005 13:44:12 -0600
Subject: [Microscopy] LaB6 filament use

Contents Retrieved from Microscopy Listserver Archives
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I would like to switch our JEOL 1010 microscope to LaB6 filaments for
biological applications and I was wondering what other people felt about
LaB6 filaments, and how much they liked them. Any comments on the matter
would be welcome because I have no experience with these filaments.

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 15:43:45 2005



From: :      Colin.Veitch-at-csiro.au
Date: Sat, 19 Feb 2005 08:44:34 +1100
Subject: [Microscopy] Screens on a Hitachi S4100 FESEM

Contents Retrieved from Microscopy Listserver Archives
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Hi,

We have a Hitachi S4100 FESEM which has been our workhorse for 12 years or so. Gradually over time the CRT screens have been getting duller and fuzzier to the point now that focussing is becoming a little difficult. Given that the machine has been used for virtually every workday since it was installed this isn't too bad.

What I'd like to know is what is the best solution to the problem? The screens are embedded in tbe console, so do we have to replace them with similar screens or can we somehow take the signal out and use an external monitor or possibly even an LCD screen? There are BNC outputs on the back so I'm guessing that it could be done.

Thank you very much for any help with this.

Colin Veitch

CSIRO Textile and Fibre Technology
Belmont Vic Australia



From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 16:16:23 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 18 Feb 2005 14:30:43 -0800
Subject: [Microscopy] Re: LaB6 filament use

Contents Retrieved from Microscopy Listserver Archives
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On Feb 18, 2005, at 11:44 AM, Garry Burgess wrote:

} I would like to switch our JEOL 1010 microscope to LaB6 filaments for
} biological applications and I was wondering what other people felt
} about
} LaB6 filaments, and how much they liked them. Any comments on the
} matter
} would be welcome because I have no experience with these filaments.
}
Dear Garry,
We have a LaB6 on our T12, and, in general, the increased brightness,
coherence, and lifetime with respect to a W filament makes it worth the
increase in cost. We have had some uneven lifetime issues with some of
the filaments we've installed, so I recommend researching which brand
of filament works best in the 1010.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 17:01:05 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 18 Feb 2005 15:01:40 -0800
Subject: [Microscopy] Specimen Preparation Technician Job Opening at Intel

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Specimen Preparation Technician Job Opening at Intel

Responsibilities:

California Materials Technology Labs (CA MATTEC), part of CTM Quality and Reliability, is providing a challenging opportunity for a Transmission Electron Microscopy (TEM) technician. As a TEM Technician, you will be responsible for carrying out TEM sample preparation of materials related to CTM/D2 development and manufacturing. Sample prep requires a high degree of manual skill, judgment and dexterity. Sample prep tools which must be operated include precision mechanical sectioning and polishing equipment, Dual Beam FIBs, Argon ion mills, and various wet etches. Additional technical duties for this position include, but are not limited to, development, evaluation and improvement of instruments and preparation techniques; minor maintenance of instruments and labs; and writing brief summary reports.

Qualifications:

This position requires an individual who can work with minimal supervision and is able to make sound independent judgments with regard to routine instrument problems, lab or analysis related issues. Excellent interpersonal and communication skills are required for interfacing with requesters, vendors, lab staff and peers. Requires an AA/AS degree with at least 2 years of lab experience. Familiarity with device fabrication and structure is regarded as a real asset for this position; previous mechanical polishing and FIB experience is also highly desirable.

Please submit any resume as a text message pasted into an e-mail, rather than as an attachment to an email.

Hiring Manager:  David W. Susnitzky
david.susnitzky-at-intel.com
Phone: 408-765-2026




From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 17:06:55 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sat, 19 Feb 2005 10:07:42 +1100
Subject: [Microscopy] Re: Chilling Water the TEM Blues

Contents Retrieved from Microscopy Listserver Archives
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Frank

Have you removed and cleaned the in-line filter on the system. ?
I've had this problem many times with 420 electronics and cleaning this
usually cures the issue.

On my 420, the inline filter is hidden in a small brass "y shaped"
connection on the inside of the right hand
side of the electronics rack. Take off the side panel, swing open the
electronics
and look in the rear lower corner. Some 400 series instruments have this
filter mounted on the lower back panel (much easier to get at).

One leg of the Y fittinghas a cap that unscrews and a metal screen
filter can be withdrawn
cleaned and then re-inserted. You'll need a pan to catch the drainage, while
your cleaning the filter. What I do is put a low profile pan underneath
unscrew the cover, remove the filter and quickly screw the cover back
on to stop a large loss of water. Clean the filter and then replace.

BTW, getting the cover off isn't always easy as it is in an awkward
location and getting a wrench on it to get leverage is inconvenient
to say the least.

Nestor
Your Friendly Neighborhood SysOp



At 4:02 PM -0500 2/17/05, frank.karl-at-degussa.com wrote:
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From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 17:17:00 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sat, 19 Feb 2005 10:18:05 +1100
Subject: [Microscopy] Re: Screens on a Hitachi S4100 FESEM

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Colin

On ANL's on the S4700 the BNC output is NTSC Video and only provides
the SEM image from the framestore. It does not provide the other
screen information.

This may be different on the 4100 series, but I tend to doubt that, as I've
seen earlier models (4500's) that use the same architecture and would
guess that the 4100 is similiar.

The important thing is that it is likely NTSC and not the PAL standard
used by most video monitors used downunder. You should however be
able use an external
monitor to focus since the output is from the framestore which has a seperate
line out.

Remember the location of the monitor is important from the ergonomics point
of view. I'm very sensitivite to neck problems when too many monitors are
positioned at the wrong viewing location/angle.

Given that this is your workhorse I'd go for replacement monitors from Hitachi.


Nestor
Your Friendly Neighborhood SysOp
(who is still in Oz)



At 8:44 AM +1100 2/19/05, {Colin.Veitch-at-csiro.au} wrote:
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From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 17:56:33 2005



From: laloggia.brigette-at-mbco.com (by way of MicroscopyListserver)
Date: Sat, 19 Feb 2005 10:58:00 +1100
Subject: [Microscopy] viaWWW: Wanted Fluorescence Microscopy short course

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Below is the result of your feedback form
(NJZFM-ultra-55). It was submitted by
(laloggia.brigette-at-mbco.com) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
on Thursday, February 17, 2005 at 12:11:08
---------------------------------------------------------------------------

Email: laloggia.brigette-at-mbco.com
Name: Brigette La Loggia

Organization: Miller Brewing

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I am a new microbiologist at Miller and
my department heads want me to take a basic
Fluorescence Microscopy short course but also by
the end of March. I was registered with McCrone
Reaseach Institute in Chicago for their Course
1210 class but due to low enrollment was
cancelled. I was hoping that you could give me
some leads on other courses. McCroneís course
consisted of introduction to theory and practice;
introductory fluorescence investigations;
fluorescence microscopes; filter sets and
cleaning; intrinsic, general, and specific
fluorescence probes; viability probes; sample
preparation; dye loading; indirect
immunolocalization; problem solving; resources,
materials and probes; and where to purchase
filters probes, and antibodies. The senior
microbiologist said that I donít want anything
that gets into image analysis. Thank you so much
for your help.

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 17:57:56 2005



From: fleyte-at-imp.mx (by way of MicroscopyListserver)
Date: Sat, 19 Feb 2005 10:59:15 +1100
Subject: [Microscopy] viaWWW: seeking microscope vibration isolation company

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form
(NJZFM-ultra-55). It was submitted by
(fleyte-at-imp.mx) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html
on Thursday, February 17, 2005 at 12:55:16
---------------------------------------------------------------------------

Email: fleyte-at-imp.mx
Name: Florentino

Organization: IMP

Title-Subject: [Microscopy] [Filtered] seeking microscope vibration isolation company

Question: Hello everyone,

I am looking for a reliable company to help us on
a high resolution microscope vibration isolation
situaciÛn we have.

The microscope we are dealing with is a high
resolution TEMF30ST from FEI, the operation
voltaje is 300 KV, it has several signal
detectors like EDS EELS HAADF and it is capable
to produce tomography images from the sample, it
has also a STEM unit and a FIELD EMISSION GUN. It
requires an ultra high vacuum system and a
cooling system.

The mic¥s own isolation system has a 2.7 Hertz
natural frequency and the concrete pad under it
has 3.1 Hertz, so we have amplification problems.
If you need more info or If you know or belong to
a company that may have low natural frequency
vibration isolation systems suitable for these
mics please conctact me at fleyte-at-imp.mx

Thank you

Florentino Leyte

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 17:58:34 2005



From: neily-at-nprl.ph.bham.ac.uk (by way of MicroscopyListserver)
Date: Sat, 19 Feb 2005 10:59:52 +1100
Subject: [Microscopy] viaWWW: STEM probe size measurement

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form
(NJZFM-ultra-55). It was submitted by
(neily-at-nprl.ph.bham.ac.uk) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html
on Friday, February 18, 2005 at 06:31:40
---------------------------------------------------------------------------

Email: neily-at-nprl.ph.bham.ac.uk
Name: Neil Young

Organization: Birmingham University UK

Title-Subject: [Microscopy] [Filtered] STEM probe measurement

Question: Dear all,

I'm looking for methods to measure the diameter
of my STEM probe at different spot sizes.Ý The
machine is an FEI tecnai F20 TEM/STEM.Ý I am
considering scanning across MgO cubes and taking
the HAADF intensity profile acoss edges of MgO
cubes.Ý Could anyone comment on this or give a
better method?Ý

regards

Neil

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 21:54:33 2005



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Fri, 18 Feb 2005 22:52:32 -0500
Subject: [Microscopy] Re: viaWWW: seeking microscope vibration isolation company

Contents Retrieved from Microscopy Listserver Archives
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Florentino,

Here are 2 companies that have active vibration isolation systems. Others
on the list may know of more vendors. I have not used either system, so I
can not make any recommendations as to which is better. For your
frequencies, passive vibration isolation will probably not give you
sufficient attenuation, I think you will need an active vibration
compensation system.

Halcyonics
http://www.halcyonics.com/
TMC
http://www.techmfg.com/

Good luck,
Henk Colijn

At 06:59 PM 2/18/2005, fleyte-at-imp.mx wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
OSU Campus Electron Optics Facility www.ceof.ohio-state.edu
040 Fontana Labs, 116 W. 19th Ave




From MicroscopyL-request-at-ns.microscopy.com Fri Feb 18 23:41:56 2005



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 18 Feb 2005 19:43:01 -1000 (HST)
Subject: [Microscopy] MM2005 - Interisland airfare deals

Contents Retrieved from Microscopy Listserver Archives
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Hi, All-

There is a sudden and limited-time-only airfare war and drop in prices
between islands on Hawaiian Airlines and Aloha Airlines. This would be
your best opportunity to book flights to the neighbor islands before or
after Microscopy & Microanalysis 2005 in Honolulu this summer. Fares are
as low as $69 each way, and we haven't seen anything like that in quite
awhile

Both airlines also have decent fares from selected cities, mostly on the
West Coast. I suspect Hawaii will have a banner tourist season this year,
so I recommend looking at fights and hotel packages sooner rather than
later.

More tips as I hear about them!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From MicroscopyL-request-at-ns.microscopy.com Sat Feb 19 01:52:11 2005



From: Allen R. Sampson :      ars-at-sem.com
Date: Sat, 19 Feb 2005 01:51:38 -0600
Subject: [Microscopy] RE: Transport of a TEM - advice needed

Contents Retrieved from Microscopy Listserver Archives
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I'm getting in on this a little late, due to hardware failures here that
prevented my getting this message till now. But, I hope that I may be able
to offer some general guidelines that will help you and others.

Any electron microscope is composed of two or more basic cabinets or
assemblies. For an SEM it might be the electron optics console and the
electronics console and may include a separate power console. A TEM may
split into the electron optics, right, left and camera assemblies and will
probably have a separate power cabinet. In either case, the manufacturer
has to design them that way in order to ship them to customers and get them
through standard doors.

The trick is to take a practical, kind of engineered, view of the required
move. If the instrument is merely being moved to a new location down the
hall, it may be practical to leave the various consoles connected, if they
can fit through the doors, and you have a number of people to push and
control the various components. This isn't as simple as it may seem - a
first step would be to provide a flexible control between components so
that they can't be separated enough to stress the electrical and other
connections between them. A rope or other means to prevent that and some
means to prevent the cables and tubing from dragging on the floor.

You would also have to take a close look at the path you will take. The
casters on instruments, or hand trucks used to transport them, are
generally small. Any bump or gap in the floor can provide a major
impediment to these. Two things have to be remembered here - these
instruments have considerable mass and the electron optics column can have
a considerable moment arm. If you try to forcibly push it over a small
bump or depression it will result in a considerable vertical and rotational
momentum. The electron optics console usually has a very high center of
gravity and such movement can easily cause a tipping motion.

That brings us to one very basic consideration. When originally shipped,
every instrument has some column clamp down mechanism. For vibration
isolation, electron optic columns are usually isolated by some form of
elastomeric mounting. That usually takes the form of the entire, and very
heavy, electron optics column, sample chamber and vacuum system being
suspended from a single plane around table height. The manufacturer
probably shipped the instrument with a number of heavy bolts that were used
to clamp the column to the table at that plane so that the column moves
with the supporting table. Without those clamps, the column can, with it's
momentum, exaggerate any rotation force and, with its high center of
gravity, cause a tip over or sufficient movement to cause damage.

If a more complicated move is required, the various cabinets, consoles or
assemblies will have to be separated. First rule here is to document every
disconnect as well as possible. Second rule, make every disconnect at the
easiest possible site. Your question regarding the high voltage cable is
quite simple in this regard. The high voltage tank connection is normally
a custom plug and connector that can be easily removed. If it results in
an opening to the tank, cover it up - heavy aluminum foil can work well
here. Given the mass and size of the tank, and the cabinet it is in, it is
unlikely that they will be subjected to any great tilting that may cause a
spill - just ensure that no contaminants will fall in. Make sure to
protect the HV cable connector, this will be a high expense part - bubble
wrap usually works good.

One thing that I pay particular attention to when I wrap up an instrument
to be transported by others is the human factor. Whether the instrument
will be secured on pallets or moved by hand, you have to pay attention to
what may be construed as a handle. Table tops should be removed - they are
usually particle board or thin metal and easily damaged. When removed,
structural members will be exposed that are suitable and obvious as
handles. Anything sticking out from the column such as aperture
micrometers, detectors and sample exchange mechanisms should be removed or
covered (a thick bubble wrap works well here) and marked to prevent anyone
from thinking that it may offer a solid handle. Basically, if someone
grabs a soft and giving surface, like an aperture micrometer wrapped with a
few layers of bubble wrap, they won't think it a suitable handle. But if
they see and can grab a structural steel tube that's solidly attached,
they'll use it.

That brings us to a real move - one where every contingency must be
covered. This is one you don't want to do yourself. If the instrument is
being transported by unknown means, without direct control and oversight,
you should dismantle the electron optics column completely and assure the
individual components are packaged to prevent damage. The problem here is
that, not only do you have to know what you are doing in disassembly, but
you have to be able to re-assemble and align the components on
installation.

Just a quick response, but hopefully one that will equip anyone planning a
move with a start on the things that should be considered.


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
Saint Charles, Illinois 60174
phone (630) 513-7093 fax (630) 513-7092
email mailto:ars-at-sem.com web www.sem.com


On Tuesday, February 15, 2005 5:51 PM, John Brealey
[SMTP:john.brealey-at-imvs.sa.gov.au] wrote:
}
}
}
------------------------------------------------------------------------
------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
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}
------------------------------------------------------------------------
-------
}
} Hi all,
}
} I have a few questions regarding the transport of a Hitachi H-600.
} I am currently seeking to have the TEM dismantled and moved from
Melbourne
} to Adelaide (800km) by a removalist company specialising in sensitive
} freight.
} Should the HV cable be removed from the gun and HV tank or can it be left
} attached at one or both ends?
} If it can be left attached at one end, which end is best to leave
attached?
} If the HV cable is removed from the HV tank, should the tank be drained
of
} oil or left full and covered by the blanking plate?
} How can the HV cable best be wrapped to minimise damage?
} How long would it take to dismantle the TEM and what is the minimum
number
} of component parts that the TEM can be broken down to? For example, is
left
} console, right console, camera console, column, HV cable, HV tank and
} electronics box a reasonable breakdown of components (this is excluding
} rotary pumps, compressor and desiccator)?
}
} Any advice or personal experiences on this issue welcome.
}
} Thanks,
}
} ____________________________
} John Brealey
} Medical Scientist
} Electron Microscopy Unit
} The Queen Elizabeth Hospital
} IMVS - TQEH Pathology
} Woodville, 5011
} Adelaide
} South Australia
} (08) 82226612
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Sat Feb 19 06:33:44 2005



From: Reinhard Rachel :      reinhard.rachel-at-biologie.uni-regensburg.de
Date: Sat, 19 Feb 2005 13:34:23 +0100
Subject: [Microscopy] TEM - LaB6 filament use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Garry,
As long as you have got the money for the LaB6 filament,
as long as the vacuum is good in the column and in
particular in the gun area (keep the oil diff or turbo pump and ion
getter pump running all the time, overnight and over weekend; always
use LN2 trap),
as long as you have well trained users,
there are a number of advantages, as Bill said:
increased brightness,
increased coherence,
increased lifetime (in our CM12, usually around 3 years; exclusively in
TEM low dose imaging modus; no EDX or EELS).

'Disadvantages':
- a good vacuum is a prerequisite
- needs careful, slow heating
- some of the users will never see how such a filament looks like (so,
keep an old one, for demo), and how to change it.

yours,
Reinhard

-------------------------------
PD Dr.Reinhard Rachel
Universität Regensburg
Lehrstuhl für Mikrobiologie
Universitaetsstr. 31
D-93053 Regensburg
tel.: +49 941 943 4534
fax.: +49 941 943 1824




From MicroscopyL-request-at-ns.microscopy.com Sat Feb 19 16:40:56 2005



From: S. Kelly Sears :      sksears-at-eps.mcgill.ca
Date: Sat, 19 Feb 2005 17:42:03 -0500
Subject: [Microscopy] Help with a JEOL-JEM 2011 w/FasTEM]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a JEOL JEM-2011 w/ FasTEM. I was wondering if anyone has created
a help file, or even better, a reference manual for using the FasTEM? I
always have the feeling that I'm not using this program to its full
capability. Many thanks.

-- Kelly

--
S. Kelly Sears, Ph.D., B.F.A.
Facility for Electron Microscopy Research
McGill University




From MicroscopyL-request-at-ns.microscopy.com Sat Feb 19 20:15:40 2005



From: Chaoying Ni :      cni-at-UDel.Edu
Date: Sat, 19 Feb 2005 21:16:16 -0500 (EST)
Subject: [Microscopy] Re: Help with a JEOL-JEM 2011 w/FasTEM]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kelly,

I'm very interested in this too. We have a FasTEM 2010f with a remote
5-axis stage control. The remote tilts have only been used once or twice
with marginal success since 2001. I probably have to disable the remote
tilts to enable motorized apertures.

A bit frustrated,
-Chao


On Sat, 19 Feb 2005, S. Kelly Sears wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} We have a JEOL JEM-2011 w/ FasTEM. I was wondering if anyone has created a
} help file, or even better, a reference manual for using the FasTEM? I always
} have the feeling that I'm not using this program to its full capability. Many
} thanks.
}
} -- Kelly
}
} --
} S. Kelly Sears, Ph.D., B.F.A.
} Facility for Electron Microscopy Research
} McGill University
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Sun Feb 20 17:09:55 2005



From: Hong Yi :      hyi-at-emory.edu
Date: Sun, 20 Feb 2005 18:14:06 -0500
Subject: [Microscopy] (Microscopy) Carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Everyone;

           We have been using Formvar/carbon coated grids for spreading
magnetic nano-particles in water and for negative staining. But as you
know aqueous solution does not spread well on grids because of the
charge characteristics on film surface. The only way I know of for
making the film surface more hydrophilic is to do glow discharge on a
sputter coater, but we do not have such a device. I heard treating
coated grids with EtOH vapor works too but did not have a very good
luck with it when I tried. Does anyone out there have any other tricks
or suggestions? Does anyone out there want to get rid of an old sputter
coater?

           Thank you in advance.

 
Hong

Emory EM




From MicroscopyL-request-at-ns.microscopy.com Sun Feb 20 18:34:17 2005



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 20 Feb 2005 16:43:02 -0800
Subject: [Microscopy] Re: (Microscopy) Carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hong -

Do you have a vacuum evaporator? It's simple to rig it for glow
discharge with an inexpensive Tesla coil. A plastic vacuum
dessicator, the same Tesla coil, and a rough vacuum source will do
the job also.

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html


From MicroscopyL-request-at-ns.microscopy.com Sun Feb 20 19:00:05 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sun, 20 Feb 2005 20:04:18 -0500
Subject: [Microscopy] TEM: Carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hong Yi wrote:
================================================================
We have been using Formvar/carbon coated grids for spreading magnetic nano-
particles in water and for negative staining. But as you know aqueous
solution does not spread well on grids because of the charge
characteristics on film surface. The only way I know of for making the film
surface more hydrophilic is to do glow discharge on a sputter coater, but
we do not have such a device. I heard treating coated grids with EtOH vapor
works too but did not have a very good luck with it when I tried. Does
anyone out there have any other tricks or suggestions? Does anyone out
there want to get rid of an old sputter coater?
================================================================
I don't think that a sputter coater, old or new, is going to solve your
problem. I have not heard of EtOH vapors making a carbon grid more
hydrophilic.

Carbon coated grids lose their hydrophilic nature as they age and they
become more hydrophobic. The process can be "reversed" by a) exposure to an
RF "air" plasma in a small plasma etcher such as the SPI Plasma Prep™ II
unit (effect will last 60-90 days) or b) a thin evaporation of Victawet®
onto the grids, see URL
http://www.2spi.com/catalog/spec_prep/evapor_3c.shtml Our own studies
would suggest that Victawet can keep the grids highly hydrophilic
essentially forever (e.g. more than one year). Just remember that it is a
phosphate based surfactant so if you are doing elemental analysis work, you
might not want to have P showing up in your data. But if you have an
ordinary vacuum evaporator and tungsten baskets, and don't have a plasma
etcher, you can solve your problem with Victawet.

The best bet for having carbon coated grids with the greatest hydrophilic
characteristics is to make or purchase your carbon coated grids always
"fresh". If the grids are purchased, and their age is uncertain, contact
the manufacturer of the carbon coated grids, give them the lot number and
then you will know.

Disclaimer: SPI Supplies manufactures the SPI Plasma Prep II Plasma Etcher,
has been a distributor of Victawet for electron microscopy applications, and
we are a manufacturer of carbon coated grids.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Sun Feb 20 23:56:10 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 20 Feb 2005 22:00:25 -0800
Subject: [Microscopy] EBSD Os coated

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Has anyone done EBSD of Os coated specimens
for EBSD? Considering the EBSD interactive
volume is about 30-50nm, the coating must be
very thin. Has anyone had success with this
Os and care to share with us about this?

Right now, I tend to stick to VP. If the
resistance of the Os was high, that would
help. SPI is of no help in this regard.
They have not done this sort of work before.

New ground for new inputs.

gary g.



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 04:18:15 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Mon, 21 Feb 2005 06:51:43 -0330
Subject: [Microscopy] RE: EBSD Os coated

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary Gaugler writes ...

} Has anyone done EBSD of Os coated specimens
} for EBSD? Considering the EBSD interactive
} volume is about 30-50nm, the coating must be
} very thin. Has anyone had success with this
} Os and care to share with us about this?

Maybe we should begin with the "benefits" for EBSD?
I would think a high-Z coating would decrease the
contrast of the EBSP(?)

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 07:45:40 2005



From: James Chalcroft :      jchalcro-at-neuro.mpg.de
Date: Mon, 21 Feb 2005 14:49:08 +0100
Subject: [Microscopy] (Microscopy) Carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Hong,

Another method occasionally used to make C-coated grids hydrophilic is to expose them for some minutes to the direct illumination of a UV lamp.
This is done at normal atmospheric pressure, so needs no vacuum technology.
Perhaps this method is applicable in your case?
Best wishes and good luck,

Jim


-----Original Message-----
} From: Hong Yi [mailto:hyi-at-emory.edu]
Sent: Monday, February 21, 2005 12:14 AM
To: Microscopy-at-microscopy.com

Dear Everyone;

           We have been using Formvar/carbon coated grids for spreading
magnetic nano-particles in water and for negative staining. But as you
know aqueous solution does not spread well on grids because of the
charge characteristics on film surface. The only way I know of for
making the film surface more hydrophilic is to do glow discharge on a
sputter coater, but we do not have such a device. I heard treating
coated grids with EtOH vapor works too but did not have a very good
luck with it when I tried. Does anyone out there have any other tricks
or suggestions? Does anyone out there want to get rid of an old sputter
coater?

           Thank you in advance.

 
Hong

Emory EM






From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 09:55:33 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 21 Feb 2005 07:59:18 -0800
Subject: [Microscopy] Re: EBSD Os coated

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The specimens are Cu IC runners. Current is passing
through them, so any sort of good coating scheme is
not workable. I don't have C coating and even so, it
would mess up the specimens. Au/Pd is too conductive
and also does indeed kill patterns.

The runners are between 0.15u and 0.25u wide and between
4-10u long. Usually, I don't look at the whole length of
the runner--just the ends and a bit beyond. Forward scatter
detector is good for imaging at lower mag, but at 100KX,
it is tricky to optimize WD, KV, probe current, etc. for good
patterns while obtaining good image resolution. Generally,
if I don't have good image resolution, I don't get good
pattern quality.

If I use Robinson BSE, it too tends to work better at short
WD and high mag rather than longer WD at high mag. My other
option is VP. But I have not sorted this feature out well
enough to use it all the time.

I was wondering what the film characteristics are like of
Os coating. Being high Z, it would degrade patterns. But,
if the coating were thin, would it reduce charge, keep patterns,
and not dramatically interfere with isolation between adjacent
runners?

gary g.


At 03:40 AM 2/21/2005, you wrote:
} Dear Gary,
}
} I'd avoid all metal coatings as they generally kill the EBSPs.
} Can you do Carbon coating with Carbon string (rather than rods) so that
} you get a very thin layer?
}
} What is the specimen?
} If it has lots of cracks/porosity (that trap charge), then think about
} gold coating and then polishing away most of the gold to leave a smooth
} surface (with a network of conductive tracks for the charge). Some
} Geologists use this trick.
} Sometimes the charging isn't really as bad as it looks in the SE image -
} try the backscatter detector (SE electrons are easily deflected); you can
} also try lower kV, e.g. 10-15, and smaller probe currents. It all depends
} on how small the features are in your specimen and what you want to
} measure, but I've looked at uncoated polycrystalline alumina and
} quartz+pyrite specimens in high vacuum.
}
} Good luck and I'll be interested to hear how things go,
}
} Austin
}
} P.S. You've probably already guessed, but HKL is a commercial EBSD company.
}
} Dr. Austin Day
} Research Manager
} HKL Technology Aps
} Majsmarken 1
} 9500 Hobro
} Denmark
} tel: +45 96 57 26 00
} fax: +45 96 57 26 09
} www.hkltechnology.com
}
}
} -----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: 21 February 2005 07:00
} To: MSA listserver
} Subject: [Microscopy] EBSD Os coated
}
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 10:27:21 2005



From: Stefan Gunnarsson :      Stefan.Gunnarsson-at-ebc.uu.se
Date: Mon, 21 Feb 2005 17:30:17 +0100
Subject: [Microscopy] SEM - Price for XL30

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

I am wondering about a suitable price for a used Philips XL30 SEM. I
have looked around on the web for used SEMs but found nothing that
would suit this.
The SEM is from 1993, has been serviced regularly every year and is in
good condition. It was upgraded to the NT-version of the software a few
years ago. The price should be 'as is', i.e. disregarding costs for
moving and re-installation.

Thanks,

Stefan

+++++++++++++++++++++++++++++++++++++++++++++++++++++++
Stefan Gunnarsson
Uppsala universitet Uppsala University
Evolutionsbiologiskt centrum Evolutionary Biology Centre
Enheten för biologisk strukturanalys Microscopy and Imaging Unit
Norbyvägen 18A
SE75236 Uppsala, Sweden Tel & Fax: +46 - 18 471 2638
+++++++++++++++++++++++++++++++++++++++++++++++++++++++



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 10:51:33 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Mon, 21 Feb 2005 10:54:13 -0600
Subject: [Microscopy] Re: RE: (Microscopy) Carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

hong

sorry i did not get to this yesterday. there are several additional
solutions to those mentioned so far. first, unless my memory is wrong,
dogma is that grids need to be 'freshly glow discharged' to reduce
hydrophobicity. i do not remember what the definition of fresh was.
i'm hope someone on the list will be able to enlighten us on that.
also, the hydrophobicity of carbon-plastic films decreases over time.
again, my memory is that the time frame is about 2 weeks - meaning that
if the grids are more than 2 weeks old most hydrophobicity is gone.

because of the way we do preparations here, the issue of hydrophobicity
is not major, our samples usually have soluble protein present and we
centrifuge directly to the grid. these factors overcome pretty well all
hydrophobicity. if you want a poor man's solution, in place of UV
treatment you can pretreat the grids by floating them on a drop of 0.1%
polylysine or 5% alcian blue for 30 seconds to 5 minutes. polylysine is
supposed to create a positive charge on the surface of the grid. i do
not know what the mechanism of alcian blue is supposed to be. we never
have seen a difference, but then our preparation methods usually will
overcome most hydrophobicity issues anyway.

oh yeah, UV - you should give 30 minutes exposure to the light. i think
that may not have been mentioned.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Work Phone: 204-789-3313
Pager: 204-931-954
Home Phone: 204-489-6924
Cell: 204-781-1502
Fax: 204-789-3926/204-489-6924




From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 12:26:52 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Mon, 21 Feb 2005 11:13:04 -0800 (PST)
Subject: [Microscopy] Re: (Microscopy) Carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Colin, there are number of possible solutions:

1) Best solution- buy replacement CRTs from your Hitachi service rep.

2) Second best- buy replacement CRTs from Richardson Electronics
http://www.rell.com/ . Click "Catalog"; enter CRT as keyword, click "CRTs
monochrome". Most likely you will have to call Richardson directly, in order
to identify your pic. tubes. So, have the following information handy: all
information from your CRTs labels, diagonal size of the screen, neck
diameter, and number of pins. Richardson can do 2 things for you. One- to
give you brand new CRTs. The inconvenience might be that this type of CRT is
mass-produced with only short decay time phosphor. Still works, but picture
in TV mode looks a bit noisier. The other thing Richardson can do, is to
repair your existing CRTs (either replace the neck assembly, or re-coat the
phosphor, or both).

3) The cheapest solution is- to increase cathode heater voltage of your CRTs
by up to 20%. This is a gamble. Might work forever, might work for only a
few months.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane
Duluth, GA 30096
Tel. (770)232-7785
Fax (770)232-1791
Mobile (678)467-0012
www.sia-cam.com
----- Original Message -----
} From: {Colin.Veitch-at-csiro.au}
To: {Microscopy-at-msa.microscopy.com}
Sent: Friday, February 18, 2005 4:44 PM

you should try poly-L-lysine i forget the
concentration since i have retired from EM. there must
be someone out there that knows the concentration.
john

--- Hong Yi {hyi-at-emory.edu} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Dear Everyone;
}
}            We have been using Formvar/carbon coated
} grids for spreading
} magnetic nano-particles in water and for negative
} staining. But as you
} know aqueous solution does not spread well on grids
} because of the
} charge characteristics on film surface. The only way
} I know of for
} making the film surface more hydrophilic is to do
} glow discharge on a
} sputter coater, but we do not have such a device. I
} heard treating
} coated grids with EtOH vapor works too but did not
} have a very good
} luck with it when I tried. Does anyone out there
} have any other tricks
} or suggestions? Does anyone out there want to get
} rid of an old sputter
} coater?
}
}            Thank you in advance.
}
}  
} Hong
}
} Emory EM
}
}
}
}




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From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 14:18:55 2005



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 21 Feb 2005 14:22:58 -0600
Subject: [Microscopy] Re: Re: EBSD Os coated

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Your work is a bit foreign to me, and maybe for that reason I am confused.
Do I understand you are trying to put down a conductive layer, but not too
conductive a layer? Is that possible? You want enough conductivity to
prevent charging in the SEM but you want to preserve the working nature of
the IC. I would not think you can have it both ways. Would not the
conductivity needed for EM render the insulating parts of the IC
ineffective? Maybe someone more familiar with this application can speak to
this.

I think I missed the reason for doing EBSD on this sample in the first
place. Could you share it with us? It sounds like you application is rather
demanding - trying to get enough current for EBSD while maintaining
resolution. I would think that you would get enough scattering in VP mode
that you would get significant contribution from areas outside your runner.
It seems that some conductive layer would be necessary.

Now curious,
Warren

At 09:59 AM 02/21/05, you wrote:
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From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 14:31:21 2005



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 21 Feb 2005 14:35:13 -0600
Subject: [Microscopy] Kodak 4489 Suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are looking for good prices on Kodak 4489 film (2K to 4K sheets
needed) and are inquiring as to where microscopists are buying this
film these days.

Thank you.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 16:23:59 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 21 Feb 2005 14:27:43 -0800
Subject: [Microscopy] Re: Re: EBSD Os coated

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ah...you hit on the dilemma. Good conductivity
to prevent charging but not such that EBSD patterns
are diminished or eliminated and not to deter IC
operation. The insulating layers are not at issue
in this analysis. They are actually the cause of the
charging!

The reason for doing EBSD on these runners of
off-the-shelf ICs is to attempt to determine the
wearout lifetime based on electromigration of the
Cu runners.

Here is my generic abstract for the work at issue:

This IC evaluation work aims to provide a quantitative method of predicting
the lifetime of COTS ICs when used in other than commercial
applications--especially when used in military applications. Of the
numerous IC failure mechanisms, this work focuses on electromigration and
metal interconnect degradation through the use of electron backscatter
diffraction (EBSD) in the scanning electron microscope (SEM). Through the
use of EBSD analysis, the effects of accelerated stressing of interconnects
can be measured and quantified as a basis for lifetime prediction.

Since the runners are very narrow (but could be long), high
mag and high rez is needed. This follows through for 25C,
100C and 150C temperature analysis using EBSD. So far, I ignore
any charging. My TSL/EDAX EBSD does not seem to care about
charging. However, I am concerned that this is an unbounded
variable and may influence the final data. Not sure about this.

So, I'm trying to make sure that I have eliminated all possible
significant variables in this research.

gary g.




At 12:22 PM 2/21/2005, you wrote:


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From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 16:49:22 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Mon, 21 Feb 2005 14:52:39 -0800 (PST)
Subject: [Microscopy] Re: Kodak 4489 Suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

electron microscopy supplies (EMS) has always been the
best if not the cheapest source for film.
john
--- "John J. Bozzola" {bozzola-at-siu.edu} wrote:

}
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} Microscopy Society of America
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}
} We are looking for good prices on Kodak 4489 film
} (2K to 4K sheets
} needed) and are inquiring as to where microscopists
} are buying this
} film these days.
}
} Thank you.
} --
}
##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics
} Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
}
##############################################################
}
}





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From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 19:21:10 2005



From: rcmoretz-at-att.net
Date: Tue, 22 Feb 2005 01:24:16 +0000
Subject: [Microscopy] Re: Kodak 4489 Suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In addition to EMS, I have obtained my film from Ladd Research, National Graphic Supply (Albany, NY) and directly from VWR (my preferred corporate supplier).

Roger Moretz, Ph.D.
Dept. of Toxicology
Boehringer Ingelheim Pharmaceuticlas, Inc
Ridgefield, CT

--
Where the world is only slightly
less weird than it actually is.

-------------- Original message ----------------------
} From: "John J. Bozzola" {bozzola-at-siu.edu}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} We are looking for good prices on Kodak 4489 film (2K to 4K sheets
} needed) and are inquiring as to where microscopists are buying this
} film these days.
}
} Thank you.
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}




From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 20:05:13 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 21 Feb 2005 18:09:47 -0800
Subject: [Microscopy] Re: Re: (Microscopy) Carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I personally don't like glow discharge at all: it's very difficult to
reproduce (from Lab to the Lab and from hand to another hand), it depends
from the equipment and there is no control to "amount" of discharge. I
agree with John that poly-lysine treatment may help. I am using 0.5-1%
poly-lysine from any EM suppliers (don't try to make solution by himself -
I don't remember, but there is some trick how to do so). So, place EM grid
on 10 ul poly-lysine drop for 5-10 min, wash on a few drops of deionized
water, air dry - good for a month (at least, did not try for
longer). Alcyan Blue works in the similar way with similar result. As far
as I remember, results with alcyan blue depend from the batch and
manufacturer (some particular is better than another - I don't remember
which one). Of coarse, it would work for positively charged molecules
only. I hope it helps, Sergey

At 11:13 AM 2/21/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Mon Feb 21 23:13:58 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 22 Feb 2005 00:17:58 -0500
Subject: [Microscopy] TEM film vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

John J. Bozzola wrote:
===========================================================
} We are looking for good prices on Kodak 4489 film (2K to 4K sheets
} needed) and are inquiring as to where microscopists are buying this
} film these days.
===========================================================
All of the main sellers of microscopy supplies and consumables, including
SPI Supplies, Pella, Ladd and EMS in the USA and Agar in the UK and PLANO in
Germany are Kodak distributors. It has been our belief, because of the
rapid and high turnovers, that film that comes from one of the "EM
distributors" is generally more fresh than film that sat for a while on the
shelf of a (lower volume in EM film) general photographic products
distributor.

But if saving money is an objective for asking the question, SPI (and some
of the above mentioned distributors) have been offering the MACO line of
films for electron microscopy, the essentially plug-in compatible film for
4489 is MACO ES film and for SO-163, is MACO EM film. Forgive me if this
sounds too commercial, but the selling prices of the MACO film are roughly
35-40% less than the selling prices of the alternative film in the yellow
box. See URL
http://www.2spi.com/catalog/photo/maco-TEM-film1.shtml

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 02:11:46 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 22 Feb 2005 03:15:45 -0500
Subject: [Microscopy] osmium metal characteristics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Gary Gaugler wrote:
=============================================================
Has anyone done EBSD of Os coated specimens for EBSD? Considering the EBSD
interactive volume is about 30-50nm, the coating must be very thin. Has
anyone had success with this Os and care to share with us about this?

Right now, I tend to stick to VP. If the resistance of the Os was high,
that would help. SPI is of no help in this regard. They have not done this
sort of work before.


and in another message:
--------------------------------
........... Good conductivity to prevent charging but not such that EBSD
patterns are diminished or eliminated and not to deter IC operation. .......
...
=============================================================
I am not aware of anyone who would have osmium metal resistance information
of the type you are requesting.

Osmium coating is still quite new and there is not as much known about the
osmium metal coatings as for coatings of other metals. But the point is,
the layer becomes conductive at a far thinner point than for metals that are
sputtered including chromium. Typically, the coatings for high resolution
SEM work are on the order of 1 nm thick. No one to my knowledge has ever
imaged a grain size in an osmium coated sample in the OPC osmium coaters.

I can direct you to some data on URL
http://www.2spi.com/catalog/osmium-plasma-coater-demonstration.html
which was on gold surface-tagged cells viewed by BSE imaging. Now everyone
tells me that this truly goes against conventional wisdom, considering how
high Z is osmium. But as you can see, despite the high Z, the thickness is
so thin that it does not interfere with the taking of the data at all.

I don't know as much about EBSD as I should, so I feel a bit insecure
suggesting this, but if it is that thin and has such an imperceptible effect
on the BSE image, could the same be expected for the EBSD image? Isn't
there similar physics going on here?

Certainly we could do a test run for you Gary, and to set that up, you
should contact Gene Rodek, E-mail: erodek-at-2spi.com. He is our laboratory
manager and he coordinates all lab demos.

Chuck
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 03:07:06 2005



From: Niko Hellsten :      niko.hellsten-at-gmail.com
Date: Tue, 22 Feb 2005 10:11:09 +0100
Subject: [Microscopy] TEM cross-section sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello to everyone!

I have a basic question concerning sample preparation for TEM.

I've recently started working on a physics and materials lab doing TEM
(microscopy and sample preparation) and it's all new to me. Obviously
we do a lot cross-section samples of multi-thin layers.

My colleague showed me a preparation technique which includes cleaving
and polishing of the sample and finally ion milling it to transparency
(making a hole in the centre with the area surrounding the whole being
transparent because of the angle of milling).

Does anyone have any tips or otherwise helpful "basic" information
about preparing samples this way or other techniques I should
consider. The sample becomes very fragile while thinning it and
cleaning it is very difficult.

Thanks to everyone who comments.

- Niko Hellstén, engineer
Laboratoire des Matériaux et Génie du Physique
Grenoble



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 04:53:53 2005



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Tue, 22 Feb 2005 13:02:35 +0200
Subject: [Microscopy] TEM cross-section sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I do not know what you are working on but the following might help.

1) Ion thin from both sides for a short while at a low angle to get a clean sample
2) carefully remove the sample and glue one side to a slotted grid.

3) Ion mill only from the top till you get a hole.

This worked lovely for some samples I have done. Difficult to get it glued down right without contaminating it, but worth it in the end.


Since some mail do get Lost, Bounces, etc Please send a duplicate/copy of all urgent mail to:

coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gaborone
Botswana
Phone : +267 355 2462/5222
Mobile : +267 718 36547
Fax : +267 318 5097
e-mail : coetzees-at-mopipi.ub.bw


-----Original Message-----
} From: Niko Hellsten [mailto:niko.hellsten-at-gmail.com]
Sent: Tuesday, February 22, 2005 11:11 AM
To: Microscopy

Hello to everyone!

I have a basic question concerning sample preparation for TEM.

I've recently started working on a physics and materials lab doing TEM
(microscopy and sample preparation) and it's all new to me. Obviously
we do a lot cross-section samples of multi-thin layers.

My colleague showed me a preparation technique which includes cleaving
and polishing of the sample and finally ion milling it to transparency
(making a hole in the centre with the area surrounding the whole being
transparent because of the angle of milling).

Does anyone have any tips or otherwise helpful "basic" information
about preparing samples this way or other techniques I should
consider. The sample becomes very fragile while thinning it and
cleaning it is very difficult.

Thanks to everyone who comments.

- Niko Hellstén, engineer
Laboratoire des Matériaux et Génie du Physique
Grenoble





From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 05:11:16 2005



From: Bobby Hooghan :      hooghan-at-grandecom.net
Date: Tue, 22 Feb 2005 05:14:51 -0600
Subject: [Microscopy] TEM cross-section sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Niko,
Have you tried using a Focused Ion Beam system in order to prepare your
TEM/SEM cross-sectional samples? Looks like that would be the way to go in
your case.
You can contact me at Hooghan-at-grandecom.net for specific issues,
Good luck,
Bobby Hooghan

-----Original Message-----
} From: Niko Hellsten [mailto:niko.hellsten-at-gmail.com]
Sent: Tuesday, February 22, 2005 3:11 AM
To: Microscopy

Hello to everyone!

I have a basic question concerning sample preparation for TEM.

I've recently started working on a physics and materials lab doing TEM
(microscopy and sample preparation) and it's all new to me. Obviously
we do a lot cross-section samples of multi-thin layers.

My colleague showed me a preparation technique which includes cleaving
and polishing of the sample and finally ion milling it to transparency
(making a hole in the centre with the area surrounding the whole being
transparent because of the angle of milling).

Does anyone have any tips or otherwise helpful "basic" information
about preparing samples this way or other techniques I should
consider. The sample becomes very fragile while thinning it and
cleaning it is very difficult.

Thanks to everyone who comments.

- Niko Hellstén, engineer
Laboratoire des Matériaux et Génie du Physique
Grenoble






From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 07:00:26 2005



From: Chaoying Ni :      cni-at-udel.edu
Date: Tue, 22 Feb 2005 08:08:44 -0500
Subject: [Microscopy] TEM cross-section sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Best to check with the King of cross-sampling:
Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)


-Chao



-----Original Message-----
} From: Niko Hellsten [mailto:niko.hellsten-at-gmail.com]
Sent: Tuesday, February 22, 2005 4:11 AM
To: Microscopy

Hello to everyone!

I have a basic question concerning sample preparation for TEM.

I've recently started working on a physics and materials lab doing TEM
(microscopy and sample preparation) and it's all new to me. Obviously
we do a lot cross-section samples of multi-thin layers.

My colleague showed me a preparation technique which includes cleaving
and polishing of the sample and finally ion milling it to transparency
(making a hole in the centre with the area surrounding the whole being
transparent because of the angle of milling).

Does anyone have any tips or otherwise helpful "basic" information
about preparing samples this way or other techniques I should
consider. The sample becomes very fragile while thinning it and
cleaning it is very difficult.

Thanks to everyone who comments.

- Niko Hellstén, engineer
Laboratoire des Matériaux et Génie du Physique
Grenoble





From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 07:18:52 2005



From: Aghajanian,John :      aghajanian-at-nso1.uchc.edu
Date: Tue, 22 Feb 2005 08:22:20 -0500
Subject: [Microscopy] RE: Kodak 4489 Suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi John,

We purchase our 4489 from Fisher Scientific who gives us a fairly good
discount. It is not listed in their catalog or web site. If you call them, they
will get it for you.

John Aghajanian
CEMF
UConn Health Center

-----Original Message-----
From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Mon 2/21/2005 3:35 PM
To: Microscopy-at-msa.microscopy.com
Cc:
Subject: [Microscopy] Kodak 4489 Suppliers





------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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-------------------------------------------------------------------------------

We are looking for good prices on Kodak 4489 film (2K to 4K sheets
needed) and are inquiring as to where microscopists are buying this
film these days.

Thank you.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################






From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 07:32:07 2005



From: Peter Van Osta :      pvosta-at-maia-scientific.com
Date: Tue, 22 Feb 2005 14:35:31 +0100
Subject: [Microscopy] Resolution and GPCR pits and vesicles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I am looking at images from GPCR activity in cells detected by studying
GFP-barrestin in cells (http://www.xsira.com/Transfluor/science_tf.htm).

From looking at the images it seems to me that the larger "vesicles"
can easily be detected by dry objectives. The smaller "pits" however
require a high-NA immersion oil objective to be detected?

What type of objectives are people using for detecting those "pits" and
"vesicles" ?

Regards,

Peter Van Osta

----------------------------------------------
Peter Van Osta, MD

Director Imaging
MAIA SCIENTIFIC
Cipalstraat 3
B-2440 Geel, Belgium
Tel.: +32 (0)14 570 620
Mobile: +32 (0)497 228 725
Fax.: +32 (0)14 570 621
Email: pvosta-at-maia-scientific.com
Website: www.maia-scientific.com
A Harvard Bioscience Company
----------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 08:48:30 2005



From: hkonishi-at-indiana.edu
Date: Tue, 22 Feb 2005 09:52:31 -0500
Subject: [Microscopy] Reference for TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am looking for a paper, book chapter, website, or personal pdf/powerpoint
file that show sample preparation method of solid. I need it for a graduate
student who has never seen TEM instrument. Unfortunately, I am not around him,
so I cannot demonstrate sample preparation for him. I have found a protocol on
Internet, but there are no pictures. He will crash sample with
acetone/alcohol/water, ultrasonicate, put a lacy carbon in the bottle, and
then air-dry. If you know a good reference with pictures, please advise. Also,
I would appreciate it if you can provide your personal file for your lecture.

Thank you,
Hiromi Konishi
IU&JHU


From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 09:41:59 2005



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 22 Feb 2005 10:45:22 -0500
Subject: [Microscopy] TEM cross-section sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There are some good references out there for you for sample prep. I would try to get a copy of the four books from MRS Proceedings on "Specimen Preparation for Transmission Electron Microscopy of Materials". I remember three of the four volume numbers: 115, 254, 480. Although, I am biased to the fourth one in the series being the best, you should definitely try to get a hold of the first and third.

Another very good source for a collection of literature on sample prep is the South Bay Technology website.

There is also a paperback book by Peter Goodhew on sample prep that has some good pointers.


Enjoy your new profession.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)


-----Original Message-----
} From: Niko Hellsten [mailto:niko.hellsten-at-gmail.com]
Sent: Tuesday, February 22, 2005 4:11 AM
To: Microscopy

Hello to everyone!

I have a basic question concerning sample preparation for TEM.

I've recently started working on a physics and materials lab doing TEM
(microscopy and sample preparation) and it's all new to me. Obviously
we do a lot cross-section samples of multi-thin layers.

My colleague showed me a preparation technique which includes cleaving
and polishing of the sample and finally ion milling it to transparency
(making a hole in the centre with the area surrounding the whole being
transparent because of the angle of milling).

Does anyone have any tips or otherwise helpful "basic" information
about preparing samples this way or other techniques I should
consider. The sample becomes very fragile while thinning it and
cleaning it is very difficult.

Thanks to everyone who comments.

- Niko Hellstén, engineer
Laboratoire des Matériaux et Génie du Physique
Grenoble





From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 13:25:03 2005



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 22 Feb 2005 13:28:48 -0600
Subject: [Microscopy] Opinion on MACO TEM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I recently heard of the MACO film as a possible substitute for the
Kodak 4489 TEM film.

I would like to hear from researchers who are using the MACO film
(good and bad points).

Thank you, again, MSA Listers.

John
--
##############################################################
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I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
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Email: bozzola-at-siu.edu
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From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 16:30:48 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Tue, 22 Feb 2005 14:34:06 -0800 (PST)
Subject: [Microscopy] Re: Opinion on MACO TEM film

Contents Retrieved from Microscopy Listserver Archives
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i have never heard of the film but this web site maybe
useful:
http://www.2spi.com/catalog/photo/maco-TEM-film1.shtml.
exhaushtive testing i would think would be in order or
just do what should be the next step in EM go digital.
john
--- "John J. Bozzola" {bozzola-at-siu.edu} wrote:

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}
} I recently heard of the MACO film as a possible
} substitute for the
} Kodak 4489 TEM film.
}
} I would like to hear from researchers who are using
} the MACO film
} (good and bad points).
}
} Thank you, again, MSA Listers.
}
} John
} --
}
##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics
} Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
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From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 18:32:25 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 22 Feb 2005 16:22:18 -0800
Subject: [Microscopy] Re: Re: EBSD Os coated

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Thanks for the response and the feedback on my website.
It has evolved over quite a few years.

In response to your response and to those of several others,
I will fill in some data areas.

While drift is typically an issue, my system (Zeiss Supra55VP)
has drift correction for EBSD and EDS. So when collecting
patterns or spectra (maps), drift is not a problem. I've
run up to 100KX and not had any drift problems seen. A major
problem is trying to find the same runner area that was
analyzed previously! Big problem. Current solution is to
burn the runner at 30KV, 120u aperture, high current to form
an index marker.

The Cu runners are sitting on either SiO2 or organic ILD.
The issue is how to ensure that charging of the ILD (either type)
does not affect the EBSD data. If phase data is being collected,
that affects the EDS detector as well. To add insult to
injury, the specimens will be at between -25C up to 150C.
Temperature shift causes image shift. But again, this can
be removed.

As I understand EBSD, the patterns are generated from within
30-40nm of the surface. This is quite different from much
greater volumetric interaction using EDS. Consequently, if
an Os coating was about 10-20nm thick, it probably would negate
EBSD patterns. Bummer.

At 15KV, 60u high current, VP works OK at 20Pa. There is
still some charging but not too much. The issue of runner-to-runner
shorting is an issue. Thus, the coating can't be to aggressively
conductive (thick) to remove charging or it affects isolation
of adjacent runners but also affects EBSD patterns. If there was
a way to coat with MWCNTs, that might work...maybe. other metal
coatings have to be thick to withstand a 15KV, 10nA probe spot.
That thickness kills the EBSD patterns.

What I am doing now may be optimum, given the circumstances.
But I'd rather not waste a lot of time doing something one way
that would be better done some other way.

gary g.





At 04:55 AM 2/22/2005, you wrote:
} Gary,
}
} I looked at your web page. Nice job and neat photographs.
}
} I have two thoughts for you. Could you lay down something like SiO2/SiO as
} an insulator and then Au-Pd? I don't think this will work for you. Comments?
}
} I think this might partially work 'both ways' for you. If you have a
} sputter coater, do you know what the size the Au-Pd nanoparticles are that
} it puts down on your samples? Mine were 4 nms spheres using a Hummer 7.
} The size and distribution of the particles were determined by viewing a
} tilted thin section of the nanoparticles in a TEM on a polymer surface.
} Suppose yours sizes are the same. Set the coating for 2 nms. That will
} create an anti-stat coating with about 50% coverage of the surface. It
} should cut down the charging on the insulating area and be chemically
} inert. Will it cut down the charging enough? I don't know. Will Au-Pd
} nanoparticles interfer with your analysis? I don't know.
}
} I do not know what you are doing either. You said, "so any sort of good
} coating scheme is not workable." I just hope this means you are worried
} about shorting the traces with a coating and not a diffraction interference
} from a coating. Both?
}
} Let me know if this anti-stat coating technique works.
}
} Hint: If you don't know the coating size of your Au-Pd particles, start at
} 1 nm, see what happens, then put down another 1 nm, and see what happens.
} I think this anti-stat should work for you without causing a short.
}
} Paul
}
} Dr. Joy, "Don't turn your sample into a piece of jewlery." (or a busbar)
}
} At 07:59 AM 2/21/05 -0800, you wrote:
} }
} }
} } ---------------------------------------------------------------------------
} ---
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} }
} } The specimens are Cu IC runners. Current is passing
} } through them, so any sort of good coating scheme is
} } not workable. I don't have C coating and even so, it
} } would mess up the specimens. Au/Pd is too conductive
} } and also does indeed kill patterns.
} }
} } The runners are between 0.15u and 0.25u wide and between
} } 4-10u long. Usually, I don't look at the whole length of
} } the runner--just the ends and a bit beyond. Forward scatter
} } detector is good for imaging at lower mag, but at 100KX,
} } it is tricky to optimize WD, KV, probe current, etc. for good
} } patterns while obtaining good image resolution. Generally,
} } if I don't have good image resolution, I don't get good
} } pattern quality.
} }
} } If I use Robinson BSE, it too tends to work better at short
} } WD and high mag rather than longer WD at high mag. My other
} } option is VP. But I have not sorted this feature out well
} } enough to use it all the time.
} }
} } I was wondering what the film characteristics are like of
} } Os coating. Being high Z, it would degrade patterns. But,
} } if the coating were thin, would it reduce charge, keep patterns,
} } and not dramatically interfere with isolation between adjacent
} } runners?
} }
} } gary g.
} }
} }
} } At 03:40 AM 2/21/2005, you wrote:
} } } Dear Gary,
} } }
} } } I'd avoid all metal coatings as they generally kill the EBSPs.
} } } Can you do Carbon coating with Carbon string (rather than rods) so that
} } } you get a very thin layer?
} } }
} } } What is the specimen?
} } } If it has lots of cracks/porosity (that trap charge), then think about
} } } gold coating and then polishing away most of the gold to leave a smooth
} } } surface (with a network of conductive tracks for the charge). Some
} } } Geologists use this trick.
} } } Sometimes the charging isn't really as bad as it looks in the SE image -
} } } try the backscatter detector (SE electrons are easily deflected); you can
} } } also try lower kV, e.g. 10-15, and smaller probe currents. It all depends
} } } on how small the features are in your specimen and what you want to
} } } measure, but I've looked at uncoated polycrystalline alumina and
} } } quartz+pyrite specimens in high vacuum.
} } }
} } } Good luck and I'll be interested to hear how things go,
} } }
} } } Austin
} } }
} } } P.S. You've probably already guessed, but HKL is a commercial EBSD company.
} } }
} } } Dr. Austin Day
} } } Research Manager
} } } HKL Technology Aps
} } } Majsmarken 1
} } } 9500 Hobro
} } } Denmark
} } } tel: +45 96 57 26 00
} } } fax: +45 96 57 26 09
} } } www.hkltechnology.com
} } }
} } }
} } } -----Original Message-----
} } } From: Gary Gaugler [mailto:gary-at-gaugler.com]
} } } Sent: 21 February 2005 07:00
} } } To: MSA listserver
} } } Subject: [Microscopy] EBSD Os coated
} } }
} } }
} } }
} } }
} } } --------------------------------------------------------------------------
} ----
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} } }
} } } Has anyone done EBSD of Os coated specimens
} } } for EBSD? Considering the EBSD interactive
} } } volume is about 30-50nm, the coating must be
} } } very thin. Has anyone had success with this
} } } Os and care to share with us about this?
} } }
} } } Right now, I tend to stick to VP. If the
} } } resistance of the Os was high, that would
} } } help. SPI is of no help in this regard.
} } } They have not done this sort of work before.
} } }
} } } New ground for new inputs.
} } }
} } } gary g.
} } }
} } }
} } } This mail has been scanned for virus by Sybari Antigen (HKL-DK).
} }
} }
} }
} }
} }



From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 18:32:25 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 22 Feb 2005 16:36:05 -0800
Subject: [Microscopy] RE: Re: Re: EBSD Os coated

Contents Retrieved from Microscopy Listserver Archives
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I'm getting about 10fps and doing data collection
for about 1 hour using .01-.05u step size. For smaller
runners, I use 10nm step size. These go quicker since
the runner length being analyzed is shorter. This is
at 15KV, 60u, high current, SE detector, 15mm WD,
pretty much for all data collection. If I use 120u, high
current, I suspect that resolution suffers and that
negatively affects the patterns.

As posted in another msg, drift is not a problem.
Forward scatter detector use is also possibly a benefit.
I would ordinarily use Robinson BSE but I'm worried about
how the close 150C specimen might affect the plastic
scintillator piece.

Hum, you should not get hats at all. I assume you mean
that the patterns are trapezoidal. This means that your
geometry is off. If the data scan at high tilt is a square,
the polymerized area at zero degrees should also be a square.
If not, collection tilt or SEM tilt correction is off. This is
a tricky area. If you take a flat piece of paper and draw
a square on it, then tilt it to say 65 degrees, the shape is
a rectangle. The net result is that analysis results are wrong.
Grain size and diameter/area will be incorrect.

gary g.


At 01:52 AM 2/22/2005, you wrote:
} Dear Gary,
}
} We've looked at similar specimens, see
} http://www.hkltechnology.com/data/0-Cu-lines.pdf. We'd normally try to go
} for very short working distance ~5mm, relatively low probe current ~1nA
} and fast mapping ~50/s to minimise charging/drift/contamination. Specimen
} contamination (build up under the beam) can also cause drift and will
} lead to a degradation of the EBSPs, which is why you need to go fast.
} Charging can affect the EBSPs, but if you map fast enough, it shouldn't
} be a big problem.
} VP will drop your spatial resolution and you'll need a higher probe
} current to get good quality EBSPs (which also has an effect on spatial
} resolution), so I don't think it will help. For mineral phases, we find
} that 10-20 Pa will eliminate most charging effects but that we need to go
} to a larger aperture, sometimes even the 120 µm, to get reasonable EBSPs.
}
} I don't think that a metallic coating over the whole specimen
} will help. However, you could try painting an (earthed) block of silver
} dag close to the area you want to look at - but it's not easy controlling
} this and the dag could make the contamination worse; maybe gold coating
} with part of the specimen masked would be simpler.
}
} Good luck,
}
} Austin
}
} P.S. One other thing on contamination and small step size. Ian Brough
} (UMIST) showed me some beautiful witches' hats (contamination cones) on an
} Al specimen. We found that if you map upwards (i.e. against gravity), then
} the hats tend to fall downwards and away from the area you're about to look at.
}
}
} -----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: 21 February 2005 23:28
} To: Warren E Straszheim
} Cc: MSA listserver
} Subject: [Microscopy] Re: Re: EBSD Os coated
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Feb 22 22:07:42 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 22 Feb 2005 20:11:26 -0800
Subject: [Microscopy] Re: Re: EBSD Os coated

Contents Retrieved from Microscopy Listserver Archives
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Yes...I have tried that. It does seem that Os
is not going to work. So I am left with regular
imaging and with VP. Thus, the challenge is to
sort out which is best. At 50KX-100KX, this is not
all that simple.

gary g.


At 07:12 PM 2/22/2005, you wrote:
} Gary,
}
} You might try some calculations with a Monte Carlo program (Casino or
} WinXRay). They allow a layer on substrate geometry. This should give you
} some idea of the BSE depth for your situation. Depth is ~100 nm for Al,
} so I would be surprised if you get anything with any sort of Os
} layer. VPSEM sounds like the best option if available.
}
} Good luck!
} Henk Colijn
}
} At 07:22 PM 2/22/2005, you wrote:
}
}
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 09:02:10 2005



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Wed, 23 Feb 2005 09:06:07 -0600
Subject: [Microscopy] Re: Position Announcement

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Due to new regulations concieved by the newly formed Office of Equal
Opportunity at the Beckman Institute, the official search announcement
for the position below will be delayed for a period of time (probably
about two weeks). Therefore, the previously posted announcement must be
considered unofficial until further notice, and the start and end dates
of the official search will have to be adjusted accordingly.
Regards,
Karl

Karl Garsha wrote:

}
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}
} Position Announcement: Visiting Light Microscopist
} Beckman Institute for Advanced Science and Technology
} University of Illinois at Urbana-Champaign
}
} The Imaging Technology Group (ITG) at the Beckman Institute for
} Advanced Science and Technology, UIUC provides facilities for both
} microscopy and visualization to campus researchers from a broad
} spectrum of disciplines. The ITG Microscopy Suite provides a wide
} range of instrumentation, training and user support for electron,
} scanning probe and optical microscopy. Optical microscopy
} capabilities include laser scanning confocal and multiphoton
} microscopy, widefield transmitted and fluorescence microscopy,
} stereomicroscopy, computer-assisted stereology, near-field scanning
} microscopy, reflected/transmitted light micro-spectroscopy and near IR
} imaging. More extensive information about our facilities is available
} on our web site at http://www.itg.uiuc.edu.
}
} The ITG is presently conducting a search for a microscopist to serve
} as a primary contact for user training, trouble-shooting, and quality
} assurance for a Leica SP-2 confocal microscope. In addition to
} managing one of the confocal microscopes, the successful applicant
} will also help to answer research questions using all of the
} microscopy techniques available in the ITG as well as participate in
} the development of advanced imaging technologies. A more detailed
} version of the announcement is available at:
}
} http://www.itg.uiuc.edu/announcements/lightmicro.htm
}
} This is a 12-month, full-time visiting academic professional position
} with university benefits and the possibility of becoming a permanent
} position. Salary is commensurate with experience. For full
} consideration, applications should be received by March 1st. To apply
} please send a letter of interest, CV, and the contact information of
} three references to:
}
} Lori Heil
} Beckman Institute for Advanced Science and Technology Group
} 405 North Mathews Avenue
} Urbana, IL 61801
} Phone: (217) 244-0170
} Fax: (217) 244-6219
} E-mail: lheil-at-uiuc.edu
}
} Informal questions can be directed to Glenn Fried, ITG Co-director
} (gfried-at-itg.uiuc.edu; phone: 333-5493), or myself (contact information
} below). Thanks.
}
} Best Regards,
} Karl
}

--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Office: B650J
Phone: 217.244.6292
Fax: 217.244.6219
Mobile: 217.390.1874
www.itg.uiuc.edu



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 10:20:10 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 23 Feb 2005 08:23:46 -0800
Subject: [Microscopy] RE: Re: Re: EBSD Os coated

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Interesting. I don't seem to have these, or at least,
don't see them. Since the specimen is in the chamber
for a long time (several weeks), perhaps this aids?
For the first scans, the specimen is rather newly
placed in the SEM. Scans are done and then the specimen
is raised to 150C for several days. Then same scans
run again at 150C. Then cool down, stabilize for two
days and run scans again at 25C.

The ID problem (Edward Principe) is trying to find each small cluster of
runners on the die. Scans are typically 3x10x.05u
and .6x3x.01u, depending on the set of runners being
scanned. Since the die piece is about 3mmx1mm, finding
my set of five to seven runners in a sea of 90,000 runners
is not easy. Usually, I have a circle inscribed around
the sets with a wide FIB beam. Also, the FIB can be used
to quickly sweep the die to clean off any crud and ensure
any dielectric is removed from the top of the runners.

The setup you showed is great for short WD. I've "discovered"
this trick too when using the multi-stub specimen holder.

Indexing maybe seems to get worse with time per run. Not sure.
Will have to check this out. But specimen drift should not
be an issue with drift correction turned on. In the EDAX/TSL
system, drift correction is done by the OIM data collection
app. It corrects the x&y scan values to compensate for a
drifting image. I have it set to update every second.
This seems to work OK. When imaging directly in the SEM,
then it has its own drift correction feature.

Have you compared fps between the XL-30 and Supra? In the
XL-30 at 15KV, spot 5, aperture 5 15mm WD, I got about 60fps.
In the Supra at 15KV, 60u aperture, high current, 15mm WD
I get about 7fps. I figure that this difference is mostly
operator error on my part. But I don't know what that error
might be. If rates are really different, then that would be
good to know and just move on. In the XL-30, the specimen
(un-heated) was mounted on a pin stub and the stage was
tilted 70 degrees. In the Supra, I use a 45 degree pre-tilt
adapter and put the specimen on a Deben temperature stage stub
for use in the XP temperature stage. Doing this, one is limited
to longer WD since the whole stage affair blocks closer access
to the pole piece. However, having the pre-tilt makes it
more convenient in x movement--less chance of bashing the
pole piece.

I used to get postings from XL-30 list but that seems to have gone
away. I would have asked there. What is your experience with
fps between systems?

I got quite a bit of feedback from the geminisem group regarding my
failed Haskris chiller. That has been about all the action, so far.
Hopefully there will be more than just this. However, it was
very educational and helpful.

gary g.



At 01:23 AM 2/23/2005, you wrote:
} Dear Gary,
}
} Re. Hats. I really do mean contamination build up and not scan
} distortion.
} If you imagine keeping the beam on one place for a long time, say 1
} second, than in most SEMs, a cone of contamination will build up (size
} depends on time, how clean your specimen & SEM are, probe current...).
} When you scan slowly, each point builds up a small cone, if your step size
} is too small or the cones fall over, then they can affect the next row of
} EBSP measurements. In some cases faster is better, but we also lower kV
} (as you do) to get better spatial resolution and test different probe currents.
}
} I've attached an image to show the hats, etc.
}
} Best wishes,
}
} Austin
}
} P.S. The GeminiSEM user group is a great idea.



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 10:28:41 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 23 Feb 2005 08:31:57 -0800
Subject: [Microscopy] Re: Re: Re: EBSD Os coated

Contents Retrieved from Microscopy Listserver Archives
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Thanks for the feedback. Alumina does not seem to
be a problem specimen. The grains are much larger
than Cu runners and mag is way lower. I scan alumina
and sapphire at 15KV-20KV using 20Pa VP. No big
problem.

For specimens that have grains that would fit between
Au webs, that would probably be OK. for normal coating
when not doing EBSD, I use Au/Pd or Pt. These definitely
kill EBSD.

gary g.


At 02:41 AM 2/23/2005, you wrote:

} Dear Gary,
}
} Perhaps it is worth trying to apply a very thin gold coating, ~10
} Angstrom. Gold tends to form islands and not a continuous layer. These
} islands provide enough paths to remove the charge, while the uncoated
} areas in between allow transmission of the diffracted electrons. I have
} had good experience with gold (better than using carbon) on alumina samples.
}
} Rene de kloe
}
}



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 16:31:15 2005



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 23 Feb 2005 16:34:12 -0600
Subject: [Microscopy] Re: Re: viaWWW: seeking microscope vibration isolation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Florentino: I have a Stacis 2000 Active Vibration Canceling System from
TMC and it works great. My building vibrates at 15 Hz and this was
getting coupled into my SEM stage and amplified. I couldn't get a
decent image over 30KX. Now I can get images around 150KX. The system
is kinda pricey but well worth it if it makes your scope usable.

Hendrik O. Colijn wrote:

}
}
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} -------------------------------------------------------------------------------
}
}
} Florentino,
}
} Here are 2 companies that have active vibration isolation systems.
} Others on the list may know of more vendors. I have not used either
} system, so I can not make any recommendations as to which is better.
} For your frequencies, passive vibration isolation will probably not
} give you sufficient attenuation, I think you will need an active
} vibration compensation system.
}
} Halcyonics
} http://www.halcyonics.com/
} TMC
} http://www.techmfg.com/
}
} Good luck,
} Henk Colijn
}
} At 06:59 PM 2/18/2005, fleyte-at-imp.mx wrote:
}
}
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } submitted by (fleyte-at-imp.mx) from
} } http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
} } Thursday, February 17, 2005 at 12:55:16
} } ---------------------------------------------------------------------------
} }
} }
} } Email: fleyte-at-imp.mx
} } Name: Florentino
} }
} } Organization: IMP
} }
} } Title-Subject: [Microscopy] [Filtered] seeking microscope vibration
} } isolation company
} }
} } Question: Hello everyone,
} }
} } I am looking for a reliable company to help us on a high resolution
} } microscope vibration isolation situaciÛn we have.
} }
} } The microscope we are dealing with is a high resolution TEMF30ST from
} } FEI, the operation voltaje is 300 KV, it has several signal
} } detectors like EDS EELS HAADF and it is capable to produce
} } tomography images from the sample, it has also a STEM unit and a
} } FIELD EMISSION GUN. It requires an ultra high vacuum system and a
} } cooling system.
} }
} } The mic¥s own isolation system has a 2.7 Hertz natural frequency and
} } the concrete pad under it has 3.1 Hertz, so we have amplification
} } problems. If you need more info or If you know or belong to a company
} } that may have low natural frequency vibration isolation systems
} } suitable for these mics please conctact me at fleyte-at-imp.mx
} }
} } Thank you
} }
} } Florentino Leyte
} }
} } ---------------------------------------------------------------------------
} }
} }
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} OSU Campus Electron Optics Facility www.ceof.ohio-state.edu
} 040 Fontana Labs, 116 W. 19th Ave
}
}
}

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 16:52:01 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Wed, 23 Feb 2005 17:55:58 -0500
Subject: [Microscopy] Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For the second time in 2 years, we are having serious
problems with our new batches of uranyl acetate. Solutions
appear very clear and give very little contrast. Increasing
the concentration (up to 5 times!) seem to solve that
problem, but gives increased precipitation. This is
extremely frustrating, especially since the company that
sells us the product is not very responsive and claims
that they are the only source of uranyl acetate around
the US. I would appreciate any advise from other EM
people who have experienced the same problem, and
information on other sources for the chemical.
Thank you

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 18:31:29 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 23 Feb 2005 16:49:30 -0800
Subject: [Microscopy] Re: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Feb 23, 2005, at 2:55 PM, Marc Pypaert wrote:

} This is
} extremely frustrating, especially since the company that
} sells us the product is not very responsive and claims
} that they are the only source of uranyl acetate around
} the US.

Dear Marc,
I don't know whom you are dealing with, but each of the first three
companies in my list of EM supply bookmarks sells UAc. I'd suggest
checking out all the web sites and ordering from another company.
There are so many responsive EM supply companies that it is not worth
dealing with one that is not.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 20:34:09 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 23 Feb 2005 21:38:15 -0500
Subject: [Microscopy] Uranyl acetate availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Marc Payert wrote:
============================================================
For the second time in 2 years, we are having serious problems with our new
batches of uranyl acetate. Solutions appear very clear and give very little
contrast. Increasing the concentration (up to 5 times!) seem to solve that
problem, but gives increased precipitation. This is extremely frustrating,
especially since the company that sells us the product is not very
responsive and claims that they are the only source of uranyl acetate around
the US. I would appreciate any advise from other EM people who have
experienced the same problem, and information on other sources for the
chemical. Thank you
============================================================
I always get an uneasy feeling when I read something like this because it
casts a pall over all of us working hard to provide high quality chemicals
and products for microscopy and at the same time, to deal honestly with our
customers. But knowing my competitors pretty well, it would be my guess
that this was an innocent mistake, perhaps an incompletely trained employee
and it should not negatively reflect on the overall thinking of that
particular supplier.

Here are just **some** of the sources for UA in the USA:

SPI Supplies
http://www.2spi.com/catalog/chem/Uranyl_Acetate.shtml

Ladd Research
http://www.laddresearch.
com/General_Catalog/Chapter_2/ElectronStains/electronstains.html

Ted Pella, Inc.
http://www.tedpella.com/chemical_html/chem3.htm#anchor440662

EMS
http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx#22400


Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 21:19:32 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 23 Feb 2005 19:24:04 -0800
Subject: [Microscopy] Re: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marc
Give us the name of company, so we'll not repeat your "mistake". I think,
it's important for EM community to share information which may help others.
By the way, I was just thinking to order UA, so from which company I SHOULD
NOT order? I very sure your answer on this message will help to resolve
the problem with company. Good luck! Sergey

At 02:55 PM 2/23/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 22:45:44 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 23 Feb 2005 20:50:19 -0800
Subject: [Microscopy] Re: Uranyl acetate availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Charles
I think, it's quite difficult to damage a really good reputation. Everyone
has some problems with vendors. It's normal. What abnormal is when the
problem repeatedly appears with the same vendor in a limited period of
time. Then, ListServer is a good place to share this "experience", so
others will have chance to avoid troubles. If vendor is smart enough, they
will fix the problem and "thank you" this forum for pointing out their
mistake. I think, it's normal to share different experience in EM
community. Have a great night, Sergey.

At 06:38 PM 2/23/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Wed Feb 23 22:46:51 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 23 Feb 2005 20:41:01 -0800
Subject: [Microscopy] Re: Re: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bill
Do you aware that each bottle of UA is about $100+? So, your suggestion is
that someone should give up with company and spoil $400 ($200 is
replacement from another company) on it... Ok, I think, you are quite rich
person. I could not afford such "deal"... I strongly believe that every
manufacturer should be responsible for its mistakes. They should
return money with great "thank you" that Marc is not me and did not post
the company's name. If it would be me - I'll post immediately and many
manufacturers know that, so I don't have any problems with UA...
Sergey

At 04:49 PM 2/23/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 02:50:55 2005



From: Niko Hellsten :      niko.hellsten-at-gmail.com
Date: Thu, 24 Feb 2005 09:54:50 +0100
Subject: [Microscopy] TEM cross-section preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everybody!

Many thanks to everybody who answered to my question. You gave me lots
of information and ideas. And thanks for the procedures also.

To be more precise about what I'm doing and how:

Sample of 750 µm thick Si wafer on top of which are thin layers. Area
of interest is about 250 µm x 950 µm. First I've cleaved and then used
a diamond saw (blade thickness 300 µm) to cut a piece that fits into
the supporting grid (1 mm x 1,6 mm) where I make a sandwitch.

After resin fixation I polish mecanically up to 50 µm thickness. And
finally when the sample is about 50 µm thick, I move to ion thinning
to make a hole in the centre.

Anyway, thanks again to everybody

- Niko Hellstén, engineer
LMGP
Grenoble



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 07:00:18 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Thu, 24 Feb 2005 08:03:59 +0100
Subject: [Microscopy] Re: Uranyl acetate availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chuck et al.
The force of your comment is well taken. And the real
question here is the source of the UAc. Lots of folks can package and
label small amounts of a powder but where do they get it? It would
not surprise me to hear that there was only one place in the country
(world?) where hunks of uranium ore and vinegar were reacted together
to give UAc crystals which were then milled to a reasonable powder.
This stuff would be sold to SPI, Ladd, Pella, EMS, etc for resale. In
that case, if the source company switched from say cider to wine
vinegar, and the product suffered, it wouldn't matter which reseller
you tried. Now, as this message makes clear, I don't know a thing how
UAc is made. Perhaps it is easy to make and the suppliers really do
have distinct products. Do you know?

As ever,
Tobias


}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Marc Payert wrote:
} ============================================================
} For the second time in 2 years, we are having serious problems with our new
} batches of uranyl acetate. Solutions appear very clear and give very little
} contrast. Increasing the concentration (up to 5 times!) seem to solve that
} problem, but gives increased precipitation. This is extremely frustrating,
} especially since the company that sells us the product is not very
} responsive and claims that they are the only source of uranyl acetate around
} the US. I would appreciate any advise from other EM people who have
} experienced the same problem, and information on other sources for the
} chemical. Thank you
} ============================================================
} I always get an uneasy feeling when I read something like this because it
} casts a pall over all of us working hard to provide high quality chemicals
} and products for microscopy and at the same time, to deal honestly with our
} customers. But knowing my competitors pretty well, it would be my guess
} that this was an innocent mistake, perhaps an incompletely trained employee
} and it should not negatively reflect on the overall thinking of that
} particular supplier.
}
} Here are just **some** of the sources for UA in the USA:
}
} SPI Supplies
} http://www.2spi.com/catalog/chem/Uranyl_Acetate.shtml
}
} Ladd Research
} http://www.laddresearch.
} com/General_Catalog/Chapter_2/ElectronStains/electronstains.html
}
} Ted Pella, Inc.
} http://www.tedpella.com/chemical_html/chem3.htm#anchor440662
}
} EMS
} http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx#22400
}
}
} Chuck
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \
University of Massachusetts
/ / / \ \ \
Amherst, MA, 01003
/ / ___ / \ \__/ \ ____ Voice: 413 - 545 - 1533
Fax: 413 - 545 - 3243
http://www.bio.umass.edu/biology/baskin/


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 09:47:16 2005



From: Stefan Gunnarsson :      Stefan.Gunnarsson-at-ebc.uu.se
Date: Thu, 24 Feb 2005 16:50:14 +0100
Subject: [Microscopy] STEM - How to......

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

I am sitting with an FEGSEM with, along all the normal SE and BSE
detectors, a STEM detector, which in our world here is primarily meant
to be used for biological TEM samples. This actually works quite
nicely, but I am aware that the STEM detector (it is of type with one
level for darkfield in four quadrants and one level for brigthfield)
could also be useful for metallurgical and other material samples. As I
am more or less totally ignorant of STEM work in that context, I would
be very glad if someone could point to good sources of information
(preferably web-sites) on the what's, how's, and why's....

thanks in advance
Stefan


+++++++++++++++++++++++++++++++++++++++++++++++++++++++
Stefan Gunnarsson
Uppsala universitet Uppsala University
Evolutionsbiologiskt centrum Evolutionary Biology Centre
Enheten för biologisk strukturanalys Microscopy and Imaging Unit
Norbyvägen 18A
SE75236 Uppsala, Sweden Tel & Fax: +46 - 18 471 2638
+++++++++++++++++++++++++++++++++++++++++++++++++++++++



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 10:16:58 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Thu, 24 Feb 2005 08:20:21 -0800 (PST)
Subject: [Microscopy] Re: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i have never had the problem you report. i have always
gotten my UA from EMS. have always made mine up in a
4% in 50%EToH. it always worked well but did counter
stain with Pb citrate.
john
--- Marc Pypaert {marc.pypaert-at-yale.edu} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} For the second time in 2 years, we are having
} serious
} problems with our new batches of uranyl acetate.
} Solutions
} appear very clear and give very little contrast.
} Increasing
} the concentration (up to 5 times!) seem to solve
} that
} problem, but gives increased precipitation. This is
} extremely frustrating, especially since the company
} that
} sells us the product is not very responsive and
} claims
} that they are the only source of uranyl acetate
} around
} the US. I would appreciate any advise from other EM
} people who have experienced the same problem, and
} information on other sources for the chemical.
} Thank you
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
}


__________________________________________________
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From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 10:24:08 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Thu, 24 Feb 2005 08:27:08 -0800 (PST)
Subject: [Microscopy] Re: Re: Uranyl acetate availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i think we are jumping the gun based on one person
experience. we are not even sure if the problem is a
vendor or with the person using the UA. i have know
experienced techs make mistakes like not aging the UA
before use and could not figure out the problem. i
think before we accuse a vendor we need more info.
just a suggestion.
john
--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Charles
} I think, it's quite difficult to damage a really
} good reputation. Everyone
} has some problems with vendors. It's normal. What
} abnormal is when the
} problem repeatedly appears with the same vendor in a
} limited period of
} time. Then, ListServer is a good place to share this
} "experience", so
} others will have chance to avoid troubles. If
} vendor is smart enough, they
} will fix the problem and "thank you" this forum for
} pointing out their
} mistake. I think, it's normal to share different
} experience in EM
} community. Have a great night, Sergey.
}
} At 06:38 PM 2/23/2005, you wrote:
}
}
}
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} -------------------------------------------------------------------------------
} }
} } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} }
} } Marc Payert wrote:
}
} ============================================================
} } For the second time in 2 years, we are having
} serious problems with our new
} } batches of uranyl acetate. Solutions appear very
} clear and give very little
} } contrast. Increasing the concentration (up to 5
} times!) seem to solve that
} } problem, but gives increased precipitation. This is
} extremely frustrating,
} } especially since the company that sells us the
} product is not very
} } responsive and claims that they are the only source
} of uranyl acetate around
} } the US. I would appreciate any advise from other EM
} people who have
} } experienced the same problem, and information on
} other sources for the
} } chemical. Thank you
}
} ============================================================
} } I always get an uneasy feeling when I read
} something like this because it
} } casts a pall over all of us working hard to provide
} high quality chemicals
} } and products for microscopy and at the same time,
} to deal honestly with our
} } customers. But knowing my competitors pretty
} well, it would be my guess
} } that this was an innocent mistake, perhaps an
} incompletely trained employee
} } and it should not negatively reflect on the overall
} thinking of that
} } particular supplier.
} }
} } Here are just **some** of the sources for UA in
} the USA:
} }
} } SPI Supplies
}
} http://www.2spi.com/catalog/chem/Uranyl_Acetate.shtml
} }
} } Ladd Research
} } http://www.laddresearch.
}
} com/General_Catalog/Chapter_2/ElectronStains/electronstains.html
} }
} } Ted Pella, Inc.
}
} http://www.tedpella.com/chemical_html/chem3.htm#anchor440662
} }
} } EMS
}
} http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx#22400
} }
} }
} } Chuck
} }
} } ===================================================
} } Charles A. Garber, Ph. D. Ph:
} 1-(610)-436-5400
} } President
} } SPI SUPPLIES FAX:
} 1-(610)-436-5755
} } PO BOX 656
} e-mail: cgarber-at-2spi.com
} } West Chester, PA 19381-0656 USA Cust. Service:
} spi2spi-at-2spi.com
} }
} }
} } Look for us!
} } ############################
} } WWW: http://www.2spi.com
} } ############################
} } ==================================================
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} 10833 Le Conte Ave, Room 33-080
} Los Angeles, CA 90095
}
} Phone: (310) 825-1144 (office)
} (310) 206-1029 (Lab)
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}
}





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From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 10:24:35 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Thu, 24 Feb 2005 08:27:33 -0800 (PST)
Subject: [Microscopy] Re: Re: Uranyl acetate availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i think we are jumping the gun based on one person
experience. we are not even sure if the problem is a
vendor or with the person using the UA. i have know
experienced techs make mistakes like not aging the UA
before use and could not figure out the problem. i
think before we accuse a vendor we need more info.
just a suggestion.
john
ps forgive any typos.
--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Charles
} I think, it's quite difficult to damage a really
} good reputation. Everyone
} has some problems with vendors. It's normal. What
} abnormal is when the
} problem repeatedly appears with the same vendor in a
} limited period of
} time. Then, ListServer is a good place to share this
} "experience", so
} others will have chance to avoid troubles. If
} vendor is smart enough, they
} will fix the problem and "thank you" this forum for
} pointing out their
} mistake. I think, it's normal to share different
} experience in EM
} community. Have a great night, Sergey.
}
} At 06:38 PM 2/23/2005, you wrote:
}
}
}
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} -------------------------------------------------------------------------------
} }
} } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} }
} } Marc Payert wrote:
}
} ============================================================
} } For the second time in 2 years, we are having
} serious problems with our new
} } batches of uranyl acetate. Solutions appear very
} clear and give very little
} } contrast. Increasing the concentration (up to 5
} times!) seem to solve that
} } problem, but gives increased precipitation. This is
} extremely frustrating,
} } especially since the company that sells us the
} product is not very
} } responsive and claims that they are the only source
} of uranyl acetate around
} } the US. I would appreciate any advise from other EM
} people who have
} } experienced the same problem, and information on
} other sources for the
} } chemical. Thank you
}
} ============================================================
} } I always get an uneasy feeling when I read
} something like this because it
} } casts a pall over all of us working hard to provide
} high quality chemicals
} } and products for microscopy and at the same time,
} to deal honestly with our
} } customers. But knowing my competitors pretty
} well, it would be my guess
} } that this was an innocent mistake, perhaps an
} incompletely trained employee
} } and it should not negatively reflect on the overall
} thinking of that
} } particular supplier.
} }
} } Here are just **some** of the sources for UA in
} the USA:
} }
} } SPI Supplies
}
} http://www.2spi.com/catalog/chem/Uranyl_Acetate.shtml
} }
} } Ladd Research
} } http://www.laddresearch.
}
} com/General_Catalog/Chapter_2/ElectronStains/electronstains.html
} }
} } Ted Pella, Inc.
}
} http://www.tedpella.com/chemical_html/chem3.htm#anchor440662
} }
} } EMS
}
} http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx#22400
} }
} }
} } Chuck
} }
} } ===================================================
} } Charles A. Garber, Ph. D. Ph:
} 1-(610)-436-5400
} } President
} } SPI SUPPLIES FAX:
} 1-(610)-436-5755
} } PO BOX 656
} e-mail: cgarber-at-2spi.com
} } West Chester, PA 19381-0656 USA Cust. Service:
} spi2spi-at-2spi.com
} }
} }
} } Look for us!
} } ############################
} } WWW: http://www.2spi.com
} } ############################
} } ==================================================
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} 10833 Le Conte Ave, Room 33-080
} Los Angeles, CA 90095
}
} Phone: (310) 825-1144 (office)
} (310) 206-1029 (Lab)
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}
}




__________________________________
Do you Yahoo!?
Yahoo! Sports - Sign up for Fantasy Baseball.
http://baseball.fantasysports.yahoo.com/


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 11:16:41 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 24 Feb 2005 12:19:35 -0500
Subject: [Microscopy] Re: Uranyl acetate availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Chuck,

I agree with you that companies try their very best
to sell good quality products to their customers. My
intention was not to cause our supplier any harm
(hence I did not name them!). But from the replies
I am getting I can see we are not the only ones
struggling with our UA supplies. Having done EM
for 20 years, in 2 continents and 5 different countries,
this is the first time I am experiencing this kind of
problems. Our supplier will replace batches for free
when we complain, but at the same time do not
warn us in advance when they are aware of
problems with their supplies, and as a result we
lose many hours (days) of work and hundreds of $!
Can't you understand my frustration?

As Sergey puts it very well, the use of this listserver
allows us to share our good and bad experiences,
and allows us to save a lot of time for not having
to repeat other people's mistakes. Sorry if this makes
you feel uneasy. My intention was not to hurt anyone,
but simply to find a solution to our serious (and
urgent) problem. I believe this is one of the main
goals of this listserver.

Best wishes

Marc

On Wednesday, February 23, 2005, at 09:38 PM, Garber, Charles A. wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Marc Payert wrote:
} ============================================================
} For the second time in 2 years, we are having serious problems with
} our new
} batches of uranyl acetate. Solutions appear very clear and give very
} little
} contrast. Increasing the concentration (up to 5 times!) seem to solve
} that
} problem, but gives increased precipitation. This is extremely
} frustrating,
} especially since the company that sells us the product is not very
} responsive and claims that they are the only source of uranyl acetate
} around
} the US. I would appreciate any advise from other EM people who have
} experienced the same problem, and information on other sources for the
} chemical. Thank you
} ============================================================
} I always get an uneasy feeling when I read something like this because
} it
} casts a pall over all of us working hard to provide high quality
} chemicals
} and products for microscopy and at the same time, to deal honestly
} with our
} customers. But knowing my competitors pretty well, it would be my
} guess
} that this was an innocent mistake, perhaps an incompletely trained
} employee
} and it should not negatively reflect on the overall thinking of that
} particular supplier.
}
} Here are just **some** of the sources for UA in the USA:
}
} SPI Supplies
} http://www.2spi.com/catalog/chem/Uranyl_Acetate.shtml
}
} Ladd Research
} http://www.laddresearch.
} com/General_Catalog/Chapter_2/ElectronStains/electronstains.html
}
} Ted Pella, Inc.
} http://www.tedpella.com/chemical_html/chem3.htm#anchor440662
}
} EMS
} http://www.emsdiasum.com/microscopy/products/chemicals/
} tannic.aspx#22400
}
}
} Chuck
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 11:44:02 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Thu, 24 Feb 2005 10:01:53 -0800
Subject: [Microscopy] Re: Re: Re: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Feb 23, 2005, at 8:41 PM, Sergey Ryazantsev wrote:

} Do you aware that each bottle of UA is about $100+? So, your
} suggestion is that someone should give up with company and spoil
} $400 ($200 is replacement from another company) on it... Ok, I think,
} you are quite rich person. I could not afford such "deal"... I
} strongly believe that every manufacturer should be responsible for its
} mistakes. They should return money with great "thank you" that Marc
} is not me and did not post the company's name. If it would be me -
} I'll post immediately and many manufacturers know that, so I don't
} have any problems with UA...

Dear Sergey,
Actually, some companies offer smaller amounts of UAc for about $25,
so one doesn't have to be too rich to try them out. As far as spoiling
$400 is concerned, if one has a bad batch of UA, one can attempt to
recover the cost from the vendor quite apart from ordering a new
supply, and one will have to order a new bottle from somebody in order
to get well-stained specimens. I agree that the original company
should, at the very least, offer to send a new bottle of UAc to replace
the bad one, and pay for shipping the old one back (if they are to find
out why that batch was bad). If, as Marc wrote, the company was
unresponsive, then one can either swallow the loss of the money for the
UAc or go to court. I expect that the prospect of negative publicity
from being named on this list would be enough to get the company to
rectify the problem, so I would contact the president first and see if
that solves the problem before posting the company name.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 11:50:48 2005



From: S. Kuehner :      kuehner-at-u.washington.edu
Date: Thu, 24 Feb 2005 09:54:26 -0800 (PST)
Subject: [Microscopy] particle analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello-

I'm looking for information on how to make matrix corrections to WDS
analysis of {20um grains of iron oxide embedded in silcate glass. The
problem is that the totals are always 1-3% low (after correcting for Fe3+
and beam overlap into the silicate matrix following the procedure
described by Warren in an LPSC abstract). It is clearly a grain size
effect and we suspect the low totals are due to unaccounted continuum
flourescence in the particles compared to the standards. I've found a
number of articles describing methods for analyzing particles ON a
substrate but have not been able to locate info on particles embedded IN a
substrate. I suspect that I'm not the first to worry about this so any
help would be great. Thanks, sk

************************************************
....amphiboles do violence to history...
T. Feininger, 2001. (taken out of context)
****************************

Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Earth and Space Sciences ph.206-543-8393
Mail Stop 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 12:58:40 2005



From: Joe Kulik :      juk12-at-psu.edu
Date: Thu, 24 Feb 2005 14:03:22 -0500
Subject: [Microscopy] TEM: Stability of amorphous Ge test sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I plan to use an amorphous Ge sample to characterize the contrast
transfer function in our TEM.

Does anyone have a comment about the stability of a thin amorphous Ge
sample after prolonged exposure to atmosphere? Must the sample be kept
under vacuum (or in an inert atmosphere)?

-- Joe Kulik

_________________________________

Joseph Kulik
Research Associate
Materials Research Institute
The Pennsylvania State University
194 MRI Bldg
University Park, PA 16802

Telephone: 814-865-0344
Fax: 814-863-8561
e-mail: juk12-at-psu.edu
_________________________________




From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 13:09:53 2005



From: Barbara Maloney :      maloneyb-at-fiu.edu
Date: Thu, 24 Feb 2005 14:11:57 -0500
Subject: [Microscopy] tetramethlysilane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Group - can you reuse this after you you only use it once on same
type of samples?
Thanks
Barbara


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 13:41:50 2005



From: John Knowles :      john-at-microvisionlabs.com
Date: Thu, 24 Feb 2005 14:41:35 -0500
Subject: [Microscopy] SEM: Critical-Point Dryer Info. needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have recently bought a used critical-point dryer (CPD) from a second
party vendor that had no manual for the unit. The model of CPD I have
is a Bomar SPC-1500. Since The Bomar Company seems to no longer exist
(all of my internet searches have failed to turn them up so far), I am
looking for anyone that may know something about this model or even have
a manual I could copy (or pay to have copied). If you know anything
about the SPC-1500 or know anyone that has one could you please let me
know? From my initial inspection of the unit everything appears to be
in good shape, but I wound rather not risk a test run without a more
detailed inspection and knowledge of the exact start up and run
procedures. Thanks for any information any of you can give me on the
SPC-1500 or even what happened to The Bomar Company.

John Knowles
MicroVision Labs, Inc.
Burlington, MA



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 14:15:25 2005



From: Paul Voyles :      voyles-at-engr.wisc.edu
Date: Thu, 24 Feb 2005 14:17:41 -0600
Subject: [Microscopy] Re: TEM: Stability of amorphous Ge test sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

At 01:03 PM 2/24/2005, you wrote:
} I plan to use an amorphous Ge sample to characterize the contrast
} transfer function in our TEM.
}
} Does anyone have a comment about the stability of a thin amorphous Ge
} sample after prolonged exposure to atmosphere? Must the sample be kept
} under vacuum (or in an inert atmosphere)?

Ge does oxidize, and I believe the oxide is water-soluble. In my
experience, Ge thin films on Cu grids also slowly, over a period of months,
crystallize when stored at room temperature in air. I don't know if
crystallization can be slowed or avoided by storing samples at low
temperature or in an inert atmosphere.


Best wishes,
Paul Voyles

Paul Voyles
Assistant Professor
Materials Science and Engineering Department
University of Wisconsin - Madison
1509 University Ave.
Madison, WI 53706-1595
Voice: (608) 265-6740
Fax: (608) 262-8353
voyles-at-engr.wisc.edu
www.engr.wisc.edu/mse/faculty/voyles_paul.html



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 14:16:33 2005



From: azel-at-azeronline.com (by way of MicroscopyListserver)
Date: Fri, 25 Feb 2005 07:20:34 +1100
Subject: [Microscopy] viaWWW: SEM advice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (azel-at-azeronline.com) from
http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html on
Thursday, February 24, 2005 at 09:11:13
---------------------------------------------------------------------------

Email: azel-at-azeronline.com
Name: Muradov

Organization: Baku State University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: We want to get scanning electronic microscope for research
semi-conductor thin films and nanoparticles. What could you advise?
What guiding price of such devices?

Bests,
Dr.M.Muradov

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 14:16:08 2005



From: g.chau-at-ucl.ac.uk (by way of MicroscopyListserver)
Date: Fri, 25 Feb 2005 07:20:03 +1100
Subject: [Microscopy] viaWWW: SEM of Hollow Biological Sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (g.chau-at-ucl.ac.uk) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Thursday, February 24, 2005 at 06:30:16
---------------------------------------------------------------------------

Email: g.chau-at-ucl.ac.uk
Name: Garr Chau

Organization: University College London

Title-Subject: [Microscopy] [Filtered] SEM of Hollow Biological Sample

Question: Hi all,

Being a novice at SEM, I'm wondering what would be the best method of
sampling a hollow-centred tube of polymer with cells mixed within? I
want to look at the homogeneity of spread of cells throughout the
polymer tube i.e. surface morphology of cross-sections.

The tube is formed within a glass capillary, but it is unfortunately
rather flimsy and I originally thought of backing it with a molten
material, letting it set (i.e. for mechanical support) and removing
the tube from the glass before slicing cross-sections, coating in
gold and scanning using SEM.

However I was unaware of the water content removal needed. I am
aware of alternatives including SEM with a cryostage and ofcourse
environmental EM.

The problems include: removing of the tube from the glass capillary,
and slicing the tube without smearing.

What is everyone's recommended approach?

Garr

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 14:17:28 2005



From: liu_zhongyi-at-yahoo.com (by way of MicroscopyListserver)
Date: Fri, 25 Feb 2005 07:21:28 +1100
Subject: [Microscopy] viaWWW: Script for importing a binary image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (liu_zhongyi-at-yahoo.com) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Thursday, February 24, 2005 at 11:43:14
---------------------------------------------------------------------------

Email: liu_zhongyi-at-yahoo.com
Name: Zhongyi Liu

Organization: Argonne National Laboratory

Title-Subject: [Microscopy] [Filtered] MListserver: Script for importing a binary image

Question: Question: I have images acquired using TIA on Tecnai 20F
microscope and exported as binary images (.bin). For data process,
I want to import these binary images to DigitalMicrograph (DM) and
save them in the format of .dm3. Does anyone have such script at
hand? (More accurately, my real question is: does anyone know how to
use DM script to open a binary image?)
Thanks in advance.

Zhongyi Liu

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 14:17:23 2005



From: lethomps-at-us.ibm.com (by way of MicroscopyListserver)
Date: Fri, 25 Feb 2005 07:20:56 +1100
Subject: [Microscopy] viaWWW: Conductive adhesive for ion milling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (lethomps-at-us.ibm.com) from on Thursday, February 24,
2005 at 10:37:15
---------------------------------------------------------------------------

Email: lethomps-at-us.ibm.com
Name: Leslie Thompson

Organization: IBM Almaden Research

Title-Subject: [Microscopy] [Filtered] Conductive adhesive for ion milling

Question: Hello Listers-

I am in need of a conductive adhesive to attach glass substrate
samples to a stub for ion milling in the Gatan PIPS. In a perfect
world, I could buy conductive crystalbond, but I am not aware of a
product like that. I have been using conductive graphite in IPA by
applying 2 drops as small as possible (ideally just the edge of a 3
mm sample), and quickly placing the sample before it dries, which
doesn't always work. The milling is greatly improved, but the
application method and cleaning after milling leaves much to be
desired. Any suggestions?

Leslie Thompson
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099
(408) 927-3856

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 14:24:58 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 24 Feb 2005 15:23:31 -0500
Subject: [Microscopy] Re: Re: Re: Uranyl acetate availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear John,

The problem is definitely not with the
users in this case! I supervise the
work of my technicians very closely,
and would have noticed changes in the
procedures. Plus the company
has acknowledged problems with the
supplies. My question is really - are
others experiencing the same problems,
and what are they doing to solve them?
I'm not trying to accuse anybody, just
want to find a solution to a serious and
costly problem.
Best

Marc

On Thursday, February 24, 2005, at 11:27 AM, john hoffpauir wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} i think we are jumping the gun based on one person
} experience. we are not even sure if the problem is a
} vendor or with the person using the UA. i have know
} experienced techs make mistakes like not aging the UA
} before use and could not figure out the problem. i
} think before we accuse a vendor we need more info.
} just a suggestion.
} john
} --- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:
}
} }
} }
} }
} -----------------------------------------------------------------------
} -------
} } The Microscopy ListServer -- Sponsor: The
} } Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------
} --------
} }
} } Charles
} } I think, it's quite difficult to damage a really
} } good reputation. Everyone
} } has some problems with vendors. It's normal. What
} } abnormal is when the
} } problem repeatedly appears with the same vendor in a
} } limited period of
} } time. Then, ListServer is a good place to share this
} } "experience", so
} } others will have chance to avoid troubles. If
} } vendor is smart enough, they
} } will fix the problem and "thank you" this forum for
} } pointing out their
} } mistake. I think, it's normal to share different
} } experience in EM
} } community. Have a great night, Sergey.
} }
} } At 06:38 PM 2/23/2005, you wrote:
} }
} }
} }
} } ----------------------------------------------------------------------
} } --------
} } } The Microscopy ListServer -- Sponsor: The
} } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} } ----------------------------------------------------------------------
} } ---------
} } }
} } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} } }
} } } Marc Payert wrote:
} }
} } ============================================================
} } } For the second time in 2 years, we are having
} } serious problems with our new
} } } batches of uranyl acetate. Solutions appear very
} } clear and give very little
} } } contrast. Increasing the concentration (up to 5
} } times!) seem to solve that
} } } problem, but gives increased precipitation. This is
} } extremely frustrating,
} } } especially since the company that sells us the
} } product is not very
} } } responsive and claims that they are the only source
} } of uranyl acetate around
} } } the US. I would appreciate any advise from other EM
} } people who have
} } } experienced the same problem, and information on
} } other sources for the
} } } chemical. Thank you
} }
} } ============================================================
} } } I always get an uneasy feeling when I read
} } something like this because it
} } } casts a pall over all of us working hard to provide
} } high quality chemicals
} } } and products for microscopy and at the same time,
} } to deal honestly with our
} } } customers. But knowing my competitors pretty
} } well, it would be my guess
} } } that this was an innocent mistake, perhaps an
} } incompletely trained employee
} } } and it should not negatively reflect on the overall
} } thinking of that
} } } particular supplier.
} } }
} } } Here are just **some** of the sources for UA in
} } the USA:
} } }
} } } SPI Supplies
} }
} } http://www.2spi.com/catalog/chem/Uranyl_Acetate.shtml
} } }
} } } Ladd Research
} } } http://www.laddresearch.
} }
} } com/General_Catalog/Chapter_2/ElectronStains/electronstains.html
} } }
} } } Ted Pella, Inc.
} }
} } http://www.tedpella.com/chemical_html/chem3.htm#anchor440662
} } }
} } } EMS
} }
} } http://www.emsdiasum.com/microscopy/products/chemicals/
} } tannic.aspx#22400
} } }
} } }
} } } Chuck
} } }
} } } ===================================================
} } } Charles A. Garber, Ph. D. Ph:
} } 1-(610)-436-5400
} } } President
} } } SPI SUPPLIES FAX:
} } 1-(610)-436-5755
} } } PO BOX 656
} } e-mail: cgarber-at-2spi.com
} } } West Chester, PA 19381-0656 USA Cust. Service:
} } spi2spi-at-2spi.com
} } }
} } }
} } } Look for us!
} } } ############################
} } } WWW: http://www.2spi.com
} } } ############################
} } } ==================================================
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } 10833 Le Conte Ave, Room 33-080
} } Los Angeles, CA 90095
} }
} } Phone: (310) 825-1144 (office)
} } (310) 206-1029 (Lab)
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }
} }
}
}
}
}
}
} __________________________________
} Do you Yahoo!?
} Yahoo! Mail - You care about security. So do we.
} http://promotions.yahoo.com/new_mail
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 14:40:25 2005



From: Leslie E Thompson :      lethomps-at-us.ibm.com
Date: Thu, 24 Feb 2005 12:43:56 -0800
Subject: [Microscopy] Conductive adhesive for ion milling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers-

I am in need of a conductive adhesive to attach glass substrate samples to
a stub for ion milling in the Gatan PIPS. In a perfect world, I could buy
conductive crystalbond, but I am not aware of a product like that. I have
been using conductive graphite in IPA by applying 2 drops as small as
possible, and quickly placing the sample before it dries, which doesn't
always work. The milling is greatly improved, but the application method
and cleaning after milling leaves much to be desired. Any suggestions?

Thanks,
Leslie


Leslie Thompson
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099
(408) 927-3856


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 14:58:48 2005



From: Edward Calomeni :      calomeni-1-at-medctr.osu.edu
Date: Thu, 24 Feb 2005 16:02:10 -0500
Subject: [Microscopy] LM embedding resins for Bone marrow

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

Have a colleague that wants everything possible done on a sample.
Wants to rapid process clinical bone marrow biopsies in a plastic resin
(GMA?) and do routine LM (no problem), FISH (probably no problem) and
also remove the resin and extract the DNA/RNA for genetic analysis.
They are currently doing the first two on rapid EDTA decal procedures
out of paraffin embedded stuff. However the EDTA chews up the DNA into
unrecognizable pieces, rendering it useless for genetic analysis. My
question is: Is there a resin that can be completely removed (for the
DNA stuff), is rapidly processed and does not interfere with any FISH
procedures.

Thanks for your help.

Ed

Edward Calomeni
Director EM Lab
Ohio State University - Pathology
M018 Starling Loving Hall
320 W. 10th Ave.
Columbus, OH 43210-1240
614-293-5580 (office)
614-293-8806 (lab)
calomeni-1-at-medctr.osu.edu


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 15:23:18 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 24 Feb 2005 16:26:24 -0500
Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,
Is your Bomar purple? I had a purple Bomar in my lab for a while...I
don't recall the model number. It was a "hand-me-down" from another
lab here that had closed up shop. It was pretty reliable and easy to
use. One feature that I liked was that the chamber was horizontal.
It worked well for the type of specimens I was running at the time.
I never had a manual either, the original user gave me a walk
through when he brought it to me. The unit I had did have a
pressure-release safety valve. I just did everything in the logical
order for a CPD run and it worked well until the temperature gauge
gave out and since the chamber didn't have a window, I could not
longer tell when I'd crossed the critical point.
Its been a few years now since I trashed it, so the details are
fuzzy. If you have any specific questions, ask them off list. Maybe
your questions will jog my memeory.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 16:22:12 2005



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Thu, 24 Feb 2005 15:26:03 -0700
Subject: [Microscopy] Microscope Contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to know if there are any university EM laboratories that have
TEMs and or SEMs that are eight or less years old and do not have
maintenance contracts. I especially would like to hear from those people
who have Tecnai, XL-30 and 2010 instruments or equivalents and do not
carry maintenance contracts for them. If so, how are repairs and
maintenance costs paid? Are all maintenance functions performed in-house
or are all maintenance functions performed by the OEM maintenance
engineers? Is there a combination of in-house and OEM maintenance? Is
there a significant subsidy from the university for maintenance costs? As
we work toward a business model in our lab, we find that $25.00/hr internal
rate charge and a 12% subsity from the Dean for maintenance contracts is
probably too little to cover all necessary costs. I realize that
periodically the question is asked about microscope charges. However, I
would like to know what the internal charge rate is for those labs where
there is no EM maintenance contract and no subsidy.

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 16:48:05 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Thu, 24 Feb 2005 14:51:28 -0800 (PST)
Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

you might check with this person. he had a post in the
MSA achives a few years ago and mentions having one,
if he his still in the buisnes: Gib Ahlstrand :
giba-at-puccini.crl.umn.edu

--- John Knowles {john-at-microvisionlabs.com} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
}
} I have recently bought a used critical-point dryer
} (CPD) from a second
} party vendor that had no manual for the unit. The
} model of CPD I have
} is a Bomar SPC-1500. Since The Bomar Company seems
} to no longer exist
} (all of my internet searches have failed to turn
} them up so far), I am
} looking for anyone that may know something about
} this model or even have
} a manual I could copy (or pay to have copied). If
} you know anything
} about the SPC-1500 or know anyone that has one could
} you please let me
} know? From my initial inspection of the unit
} everything appears to be
} in good shape, but I wound rather not risk a test
} run without a more
} detailed inspection and knowledge of the exact start
} up and run
} procedures. Thanks for any information any of you
} can give me on the
} SPC-1500 or even what happened to The Bomar Company.
}
} John Knowles
} MicroVision Labs, Inc.
} Burlington, MA
}
}
}




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From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 16:51:30 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Thu, 24 Feb 2005 14:55:07 -0800 (PST)
Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i have found through google a refence that used the
bomar cpd. ladd sold it years back.
john
--- John Knowles {john-at-microvisionlabs.com} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
}
} I have recently bought a used critical-point dryer
} (CPD) from a second
} party vendor that had no manual for the unit. The
} model of CPD I have
} is a Bomar SPC-1500. Since The Bomar Company seems
} to no longer exist
} (all of my internet searches have failed to turn
} them up so far), I am
} looking for anyone that may know something about
} this model or even have
} a manual I could copy (or pay to have copied). If
} you know anything
} about the SPC-1500 or know anyone that has one could
} you please let me
} know? From my initial inspection of the unit
} everything appears to be
} in good shape, but I wound rather not risk a test
} run without a more
} detailed inspection and knowledge of the exact start
} up and run
} procedures. Thanks for any information any of you
} can give me on the
} SPC-1500 or even what happened to The Bomar Company.
}
} John Knowles
} MicroVision Labs, Inc.
} Burlington, MA
}
}
}




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From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 16:59:33 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Thu, 24 Feb 2005 17:03:34 -0600
Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Garr,

Fix in glutaraldehyde and dehydrate as usual for critical point
drying, but! after the final 100% EtOH, throw the tubes in liquid
nitrogen and snap them. The EtOH freezes vitreously, and you'll get
nice breaks with no smearing.
I do this for biofilm studies in catheters. Your biggest problem will
be finding the bits after breaking -- capillary tubing is pretty
small. Try putting black paper over the aluminum (oop -- "aluminium")
foil that you use the line the styrofoam container holding the LN2,
and do the breaking in small petri dishes in the LN2 bath.
Bring back into 100% EtOH and critical point dry as usual.

Phil

} Email: g.chau-at-ucl.ac.uk
} Name: Garr Chau
}
} Organization: University College London
}
} Title-Subject: [Microscopy] [Filtered] SEM of Hollow Biological Sample
}
} Question: Hi all,
}
} Being a novice at SEM, I'm wondering what would be the best method
} of sampling a hollow-centred tube of polymer with cells mixed
} within? I want to look at the homogeneity of spread of cells
} throughout the polymer tube i.e. surface morphology of
} cross-sections.
}
} The tube is formed within a glass capillary, but it is unfortunately
} rather flimsy and I originally thought of backing it with a molten
} material, letting it set (i.e. for mechanical support) and removing
} the tube from the glass before slicing cross-sections, coating in
} gold and scanning using SEM.
}
} However I was unaware of the water content removal needed. I am
} aware of alternatives including SEM with a cryostage and ofcourse
} environmental EM.
}
} The problems include: removing of the tube from the glass capillary,
} and slicing the tube without smearing.
}
} What is everyone's recommended approach?
}
} Garr
}
} ---------------------------------------------------------------------------

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 17:27:02 2005



From: John Knowles :      john-at-microvisionlabs.com
Date: Thu, 24 Feb 2005 18:26:26 -0500
Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yes, someone just emailed me and said that The Bomar Company went under
about 20 years ago. I talked to Ernest Fullem Co. and they said they
had also carried the Bomar CPDs back in the day but never had any at
their location. They would just have them sent to the customer when one
was ordered. So they have no manuals or such. That is likely the same
with Ladd as well.
Looks like there are a few manuals out there from the emails I have been
getting and I should be ok on that front. Thanks for everyone's
responses. You have been very helpful to me.

John Knowles
MicroVision Labs, Inc.


-----Original Message-----
} From: john hoffpauir [mailto:hoffpajo-at-yahoo.com]
Sent: Thursday, February 24, 2005 5:55 PM
To: John Knowles; Microscopy-at-microscopy.com

i have found through google a refence that used the
bomar cpd. ladd sold it years back.
john
--- John Knowles {john-at-microvisionlabs.com} wrote:

}
}
}
------------------------------------------------------------------------
------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
-------
}
}
} I have recently bought a used critical-point dryer
} (CPD) from a second
} party vendor that had no manual for the unit. The
} model of CPD I have
} is a Bomar SPC-1500. Since The Bomar Company seems
} to no longer exist
} (all of my internet searches have failed to turn
} them up so far), I am
} looking for anyone that may know something about
} this model or even have
} a manual I could copy (or pay to have copied). If
} you know anything
} about the SPC-1500 or know anyone that has one could
} you please let me
} know? From my initial inspection of the unit
} everything appears to be
} in good shape, but I wound rather not risk a test
} run without a more
} detailed inspection and knowledge of the exact start
} up and run
} procedures. Thanks for any information any of you
} can give me on the
} SPC-1500 or even what happened to The Bomar Company.
}
} John Knowles
} MicroVision Labs, Inc.
} Burlington, MA
}
}
}




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From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 18:20:09 2005



From: Jeffrey Farrer :      jeff_farrer-at-byu.edu
Date: Thu, 24 Feb 2005 17:24:13 -0700
Subject: [Microscopy] Visible-light Microscopes for Honduras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Looking for surplus compound or stereo visible-light microscopes for
the University of Honduras.

I received a request from a humanitarian group that has been doing a
lot of work in the Honduras, for light microscopes to be used in
setting up a microbiology department at the University of Honduras.
Other microbiology-type equipment was also requested, but I'll stick to
the microscopes for this forum.

The group that contacted me started out building homes in Honduras,
then they collaborated with another group (Academy of LDS Dentists) and
set up a Dental clinic with Dentists from the U.S. Now they are
working with the University of Honduras to expand their educational
offerings. A group of professors from North American Universities will
be going down at the end of March along with a container of donated lab
equipment. If you have any surplus visible-light microscopes (or other
microbiology equip) that you would like to donate, or if you have any
questions about this, please send an email to:

helpforhonduras-at-beprepared.com

All donated equipment is going to a 501c3 (non-profit) organization.
The group said they could pay for shipping and would provide the info
for U.S. charitable tax deductions.

Thanks

Jeff Farrer



From MicroscopyL-request-at-ns.microscopy.com Thu Feb 24 22:41:10 2005



From: James Pawley :      jbpawley-at-wisc.edu
Date: Thu, 24 Feb 2005 22:45:17 -0600
Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We had a Bomar years ago and it was famous for using freon rather than CO2.

They may have made a later model for CO2 after freon got expensive
(and now illegal) but you should check that yours is actually
designed for CO2.

Not only are the seals different but freon went through its critical
point at a much lower pressure and so it was cheaper to make the
chamber.

Cheers,

Jim Pawley

}
} i have found through google a refence that used the
} bomar cpd. ladd sold it years back.
} john
} --- John Knowles {john-at-microvisionlabs.com} wrote:
}
} }
} } I have recently bought a used critical-point dryer
} } (CPD) from a second
} } party vendor that had no manual for the unit. The
} } model of CPD I have
} } is a Bomar SPC-1500. Since The Bomar Company seems
} } to no longer exist
} } (all of my internet searches have failed to turn
} } them up so far), I am
} } looking for anyone that may know something about
} } this model or even have
} } a manual I could copy (or pay to have copied). If
} } you know anything
} } about the SPC-1500 or know anyone that has one could

--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 11-23, 2005, UBC, Vancouver Canada
Info: www.3dcourse.ubc.ca/ Applications due by March 15, 2005


From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 02:35:17 2005



From: Neil P. Young :      neil-at-young8696.freeserve.co.uk
Date: Fri, 25 Feb 2005 09:38:42 +0100 (CET)
Subject: [Microscopy] Re: STEM - How to......

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Stefan,
Have a look for the book: Characterization of Nanophase Materials, published by
Wiley, edited by Z.L. Wang. (2000). Has a nice section on STEM. Also have a look
for the papers from S. Pennycook / P.Nellist for theory on HAADF and applications.

Neil


} Message date : Feb 24 2005, 08:52 PM
} From : "Stefan Gunnarsson" {Stefan.Gunnarsson-at-ebc.uu.se}
} To : Microscopy-at-microscopy.com
} Copy to :
} Subject : STEM - How to......
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Dear listers,
}
} I am sitting with an FEGSEM with, along all the normal SE and BSE
} detectors, a STEM detector, which in our world here is primarily meant
} to be used for biological TEM samples. This actually works quite
} nicely, but I am aware that the STEM detector (it is of type with one
} level for darkfield in four quadrants and one level for brigthfield)
} could also be useful for metallurgical and other material samples. As I
} am more or less totally ignorant of STEM work in that context, I would
} be very glad if someone could point to good sources of information
} (preferably web-sites) on the what's, how's, and why's....
}
} thanks in advance
} Stefan
}
}
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
} Stefan Gunnarsson
} Uppsala universitet Uppsala University
} Evolutionsbiologiskt centrum Evolutionary Biology Centre
} Enheten för biologisk strukturanalys Microscopy and Imaging Unit
} Norbyvägen 18A
} SE75236 Uppsala, Sweden Tel & Fax: +46 - 18 471 2638
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
}
}
}


Neil Young


--

Whatever you Wanadoo:
http://www.wanadoo.co.uk/time/

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From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 02:55:41 2005



From: gillian.2.brown-at-gsk.com
Date: Fri, 25 Feb 2005 08:58:16 +0000
Subject: [Microscopy] Re:Re: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I had an interesting conversation with another experienced Manager of an
EM facility here in the UK regarding recent problems she had with UA
precipitating and actually darkening in colour in the stock bottle.
After a lot of 'research' it turned out not to be a supplier or operator
problem.
They had recently had the fluorescent tubes changed in the over head
lighting system which caused some weird photochemical effect.
They now have ensure all UA is kept in the dark and actually do the UA
staining in the dark too.

Gill Brown
GSK
Stevenage
UK





"Marc Pypaert" {marc.pypaert-at-yale.edu}
23-Feb-2005 22:55

To
Microscopy {Microscopy-at-microscopy.com}
cc

Subject
Uranyl Acetate








------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

For the second time in 2 years, we are having serious
problems with our new batches of uranyl acetate. Solutions
appear very clear and give very little contrast. Increasing
the concentration (up to 5 times!) seem to solve that
problem, but gives increased precipitation. This is
extremely frustrating, especially since the company that
sells us the product is not very responsive and claims
that they are the only source of uranyl acetate around
the US. I would appreciate any advise from other EM
people who have experienced the same problem, and
information on other sources for the chemical.
Thank you

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446








From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 07:14:51 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 25 Feb 2005 08:18:51 -0500
Subject: [Microscopy] Embedding of cells in a tube

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --


Garr Chau wrote:
===========================================================
Being a novice at SEM, I'm wondering what would be the best method of
sampling a hollow-centred tube of polymer with cells mixed within? I want
to look at the homogeneity of spread of cells throughout the polymer tube i
e. surface morphology of cross-sections.

The tube is formed within a glass capillary, but it is unfortunately rather
flimsy and I originally thought of backing it with a molten material,
letting it set (i.e. for mechanical support) and removing the tube from the
glass before slicing cross-sections, coating in gold and scanning using SEM

From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 09:58:45 2005



From: Gib Ahlstrand :      ahlst007-at-tc.umn.edu
Date: Fri, 25 Feb 2005 10:08:36 -0600
Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John Knowles,

With respect to:

} you might check with this person. he had a post in the
} MSA achives a few years ago and mentions having one,
} if he his still in the buisnes: Gib Ahlstrand :
} giba-at-puccini.crl.umn.edu

Yep, I am still in the biz, but my e-mail address has changed to:

ahlst007-at-tc.umn.edu

I did have a Bomar CPD a long time ago, the SPC-50. It was a manual unit,
and you had to plumb in the cooling and heating water lines. I still have a
sales brochure but the 1500 is not mentioned in it, only the SPC-50 series
and the SPC-900 series, that's how old the brochure is. But it is not an
operating manual. The company went out of business a long time ago.

If you've operated a CPD before, you should have no trouble operating the
1500. I don't know if the 1500 has an automatic or programed mode, but I'd
suggest you run it manually at first to check it out. Carefully inspect the
chamber cover: if has a window, make sure you put it on right side facing
out, as its likely designed to support the pressure only going one way. Most
units have a fool-proof mount so that it can only be put on correctly, but
my old Bomar did not, as I recall.

PLease contact me off line if you want more specific info.

Gib
--
Gib Ahlstrand, Scientist
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-2785 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/

"You can learn a lot by observation - just by lookin'!" - Yogi Berra
----------------------------------------------------------------------------

} } I have recently bought a used critical-point dryer
} } (CPD) from a second
} } party vendor that had no manual for the unit. The
} } model of CPD I have
} } is a Bomar SPC-1500. Since The Bomar Company seems
} } to no longer exist
} } (all of my internet searches have failed to turn
} } them up so far), I am
} } looking for anyone that may know something about
} } this model or even have
} } a manual I could copy (or pay to have copied). If
} } you know anything
} } about the SPC-1500 or know anyone that has one could
} } you please let me
} } know? From my initial inspection of the unit
} } everything appears to be
} } in good shape, but I wound rather not risk a test
} } run without a more
} } detailed inspection and knowledge of the exact start
} } up and run
} } procedures. Thanks for any information any of you
} } can give me on the
} } SPC-1500 or even what happened to The Bomar Company.
} }
} } John Knowles
} } MicroVision Labs, Inc.
} } Burlington, MA



From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 10:12:09 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Fri, 25 Feb 2005 08:14:58 -0800 (PST)
Subject: [Microscopy] Re: Re:Re: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

UA precipitation is almost always linked to light. UA
should always be stored in a dark bottle, filtered
before staining and stained in the dark if staining
longer than a few minutes. when working in a collagen
lab had to stain for over 20 minutes, then the
staining had to be carried out in the dark.
john
--- gillian.2.brown-at-gsk.com wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Hi,
} I had an interesting conversation with another
} experienced Manager of an
} EM facility here in the UK regarding recent problems
} she had with UA
} precipitating and actually darkening in colour in
} the stock bottle.
} After a lot of 'research' it turned out not to be a
} supplier or operator
} problem.
} They had recently had the fluorescent tubes changed
} in the over head
} lighting system which caused some weird
} photochemical effect.
} They now have ensure all UA is kept in the dark and
} actually do the UA
} staining in the dark too.
}
} Gill Brown
} GSK
} Stevenage
} UK
}
}
}
}
}
} "Marc Pypaert" {marc.pypaert-at-yale.edu}
} 23-Feb-2005 22:55
}
} To
} Microscopy {Microscopy-at-microscopy.com}
} cc
}
} Subject
} Uranyl Acetate
}
}
}
}
}
}
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} For the second time in 2 years, we are having
} serious
} problems with our new batches of uranyl acetate.
} Solutions
} appear very clear and give very little contrast.
} Increasing
} the concentration (up to 5 times!) seem to solve
} that
} problem, but gives increased precipitation. This is
} extremely frustrating, especially since the company
} that
} sells us the product is not very responsive and
} claims
} that they are the only source of uranyl acetate
} around
} the US. I would appreciate any advise from other EM
} people who have experienced the same problem, and
} information on other sources for the chemical.
} Thank you
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
}
}
}
}
}
}




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From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 10:13:28 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 25 Feb 2005 10:17:25 -0600
Subject: [Microscopy] TEM--Ultramicrotomy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

We have someone wanting to study bacterial attachment to sand grains.
Does anyone have any hints on ultramicrotomy of sand? This will be a
first for us.

We're going to start with negative staining, hoping to avoid cutting
thins altogether, but if that doesn't work we thought we'd try adhering
to poly-lysine coated Thermonox cover slips or embedding in agar before
resin embedding.

Thanks!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 11:29:06 2005



From: Roger Baker :      rcbaker-at-eden.infohwy.com
Date: Fri, 25 Feb 2005 11:29:39 -0600
Subject: [Microscopy] Re: TEM--Ultramicrotomy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am a hands-on amateur, interested in microscopy for decades, and new
to this list.

I have been studying ultramicrotome and embedding technique in some
detail recently. It would be my guess that as you approach the hard
grain it the bacterial layer might section normally, but its an obvious
way to screw up a good diamond knife.

So I would imagine that you would fix and embed whole sand grains as if
they were a piece of tissue. And then mechanically pry away the sand
grain to separate it from the embedding plastic, leaving the bacterial
layer stuck in the exposed plastic surface, sort of like
freeze-fracture. And then you would cure a drop of fresh embedding
plastic onto this exposed surface and then slice it. -- Roger, Austin
Tx


On Feb 25, 2005, at 10:17 AM, Tindall, Randy D. wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Hi all,
}
} We have someone wanting to study bacterial attachment to sand grains.
} Does anyone have any hints on ultramicrotomy of sand? This will be a
} first for us.
}
} We're going to start with negative staining, hoping to avoid cutting
} thins altogether, but if that doesn't work we thought we'd try adhering
} to poly-lysine coated Thermonox cover slips or embedding in agar before
} resin embedding.
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 12:10:44 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Fri, 25 Feb 2005 12:58:22 -0600
Subject: [Microscopy] Re: TEM--Ultramicrotomy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr. Muradov

We are consultants in electron microscopy and one of the services that we
provide is advice on the purchase of electron microscopes and energy
dispersive analysis systems.

Our services are available in three styles which you may use as parts or as
a complete purchasing service.

1) We will discuss your requirements and finances and then survey the
current instruments that would be suitable for your establishment . We will
prepare a report with our suggestions as to the most suitable instruments.
2) Further to the above to prepare suitable samples of your material and
visit the microscope manufacturers to determine the most suitable
instruments for the tasks you wish to perform. We may carry out this
section for you and prepare a report or you may join us for this evaluation.
3) We will study the results obtained from the instruments with you and
determine which has performed in the most satisfactory manner therefore
which instrument should be purchased.

Further information on our techniques of instrument evaluation are available
on request.

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
www.emcourses.com tel +44 1280 816512 fax +44 1280 814007
Mobil +44 07711 606967

----- Original Message -----
} From: "by way of MicroscopyListserver" {azel-at-azeronline.com}
To: {microscopy-at-ns.microscopy.com}
Sent: Thursday, February 24, 2005 8:20 PM

Randy,

If you have the equipment, the best way to do this is freeze-fixing
-- plunge into slush or HPF -- then cryoSEM of the frozen sample.
Next best is freeze-fixing followed by proper freeze-drying (if you
don't have a real freeze-dryer, the kind that starts at -90 then goes
to -60, etc., you can put the samples in a vacuum desiccator with
molecular sieve, pull a good vacuum, and stick them in a -80 freezer.
The desiccant acts as a cryopump.
Next best after that is routine fix/deH2O/CPD. That won't have any
more artifacts than sectioning.
The negative staining would be useful if you get it to work.
Have fun!

Phil

} Hi all,
}
} We have someone wanting to study bacterial attachment to sand grains.
} Does anyone have any hints on ultramicrotomy of sand? This will be a
} first for us.
}
} We're going to start with negative staining, hoping to avoid cutting
} thins altogether, but if that doesn't work we thought we'd try adhering
} to poly-lysine coated Thermonox cover slips or embedding in agar before
} resin embedding.
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 13:39:24 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Fri, 25 Feb 2005 11:42:42 -0800 (PST)
Subject: [Microscopy] Re: TEM--Ultramicrotomy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

agar may not be needed if you have a large enough
number of bacteria, just spin them down in the
fixative, should get a pellet.as for the sand, well
never have cut sand not even certain it is cutable.
--- "Tindall, Randy D." {TindallR-at-missouri.edu} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Hi all,
}
} We have someone wanting to study bacterial
} attachment to sand grains.
} Does anyone have any hints on ultramicrotomy of
} sand? This will be a
} first for us.
}
} We're going to start with negative staining, hoping
} to avoid cutting
} thins altogether, but if that doesn't work we
} thought we'd try adhering
} to poly-lysine coated Thermonox cover slips or
} embedding in agar before
} resin embedding.
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small
} Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
}




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From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 13:50:21 2005



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 25 Feb 2005 14:53:43 -0500
Subject: [Microscopy] Conductive adhesive for ion milling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Are you making cross sections or plan view samples?

Regardless, I don't think that you need conductive glue for glass samples. We have been making cross sections and plan sections on glass for a number of years.

Check you alignment of the guns in the PIPS. If you use float glass (normal window glass) you can see the sample fluoresce when the beam hits it. In the limited number of glass samples that I have prepared in a PIPS (about 12 total), I have found that the focus drifted more than I liked on the machine that I was using. In addition, the PIPS guns are too hot for glass and you have to de-tune them to conditions where the rates may be a little slow, i.e. Low kV and low current. It also helps to use the lowest practical milling angle to minimize heating. (However, heating helps the conductivity of glass.) It may be that the sample holder

The best results that I get for cross sections is using a blank piece of silicon on top. (I think that you might be able to scrounge up a loose piece or two. :) ) The Si helps several ways: it helps in the mechanical thinning process to gauge the thickness of the dimple with glass -just use the color changes that John McCaffrey described and calibrated a number of years ago, it helps dissipate the heat in the ion mill, it helps with charging in the microscope, and it helps align the cross section with the surface parallel to the beam by using the [011] zone axis of Si by tilting in the TEM.

I hope this helps.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)


-----Original Message-----
} From: Leslie E Thompson [mailto:lethomps-at-us.ibm.com]
Sent: Thursday, February 24, 2005 3:44 PM
To: microscopy-at-microscopy.com

Hello Listers-

I am in need of a conductive adhesive to attach glass substrate samples to
a stub for ion milling in the Gatan PIPS. In a perfect world, I could buy
conductive crystalbond, but I am not aware of a product like that. I have
been using conductive graphite in IPA by applying 2 drops as small as
possible, and quickly placing the sample before it dries, which doesn't
always work. The milling is greatly improved, but the application method
and cleaning after milling leaves much to be desired. Any suggestions?

Thanks,
Leslie


Leslie Thompson
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099
(408) 927-3856





From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 15:21:28 2005



From: bplowman-at-pacific.edu (by way of MicroscopyListserver)
Date: Sat, 26 Feb 2005 08:25:30 +1100
Subject: [Microscopy] viaWWW: Intel Play QXP3 digital microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (bplowman-at-pacific.edu) from
http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html on
Friday, February 25, 2005 at 14:20:17
---------------------------------------------------------------------------

Email: bplowman-at-pacific.edu
Name: Barbara Plowman

Title-Subject: [Microscopy] [Filtered] MListserver:Intel Play QXP3 digital microscope

Question: Does anyone have a QXP3Plus.exe file on disk they could
send to me? I have a CD for the Windows 98 version. The Plus CD is
for XP. There is a QXP3Plus.exe file one can download from the
website, but the download keeps interrupting and I can't get the
complete file finished even with download managers. I will be happy
to reimburse anyone who can send me the complete QXP3Plus.exe file
for Windows XP. Thank you...this is for fun!

Barbara Plowman
Univ of the Pacific
Electron Microscope Lab
2155 Webster
San Francisco, CA 94115
email:bplowman-at-pacific.edu
ph: 415-929-6692

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 15:43:29 2005



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 25 Feb 2005 15:47:08 -0600
Subject: [Microscopy] RE: ultramicrotomy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

This is a real nitty gritty ultramicrotomy problem. The diamond knive
people are cringing, I'm sure.

How about treating the sand/bacteria with HF to soften the sand prior
to sectioning? It may even be possible to totally dissolve the sand
prior to embedding. Of course, then you won't be able to see how the
bacteria are attached to the sand. I asssume they are wanting to see
the "strands" or cement that the bacteria use to adhere.

Better yet, try to convince the people to culture the bacteria on
something like silica gel or even a glass side which would be easier
to deal with.

Good luck, Rando.

JB


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################


From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 19:06:20 2005



From: James Pawley :      jbpawley-at-wisc.edu
Date: Fri, 25 Feb 2005 19:10:27 -0600
Subject: [Microscopy] RE: ultramicrotomy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don't fight Mother Nature:

Better plan is to freeze dry and look at in the SEM. Alan Boyde made
spectacular stereo images of the bacterial jungle on the surface of
teeth in about 1973. Should be repeated more.

Also don't forget Confocal. There will be a whole chapter on Biofilms
in the new edition of The Handbook (Coming soon, to a theatre near
you!). You can even watch them grow, something you won't get in an EM.

Cheers,

Jim P.

}
} This is a real nitty gritty ultramicrotomy problem. The diamond
} knive people are cringing, I'm sure.
}
} How about treating the sand/bacteria with HF to soften the sand
} prior to sectioning? It may even be possible to totally dissolve the
} sand prior to embedding. Of course, then you won't be able to see
} how the bacteria are attached to the sand. I asssume they are
} wanting to see the "strands" or cement that the bacteria use to
} adhere.
}
} Better yet, try to convince the people to culture the bacteria on
} something like silica gel or even a glass side which would be easier
} to deal with.
}
Good luck, Rando.
--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 11-23, 2005, UBC, Vancouver Canada
Info: htt/www.3dcourse.ubc.ca/ Applications due by March 15, 2005


From MicroscopyL-request-at-ns.microscopy.com Fri Feb 25 19:15:32 2005



From: James Pawley :      jbpawley-at-wisc.edu
Date: Fri, 25 Feb 2005 19:19:35 -0600
Subject: [Microscopy] Re: TEM--Ultramicrotomy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,

If you don't have a good SEM, you might try to look at the bacterial
film as a whole mount. Even at 100 kV you can see through a lot of
tissue once the water is gone. Just put some sand on the grid and
grow the bacteria on it, then remove, fix, CPD (perhaps a thin
coating of carbon or metal, very thin) and view in the TEM. If you
are careful, you should have clumps sticking off the side of the sand
grains and some of these may show what you need. ANd if yo have a
TEM/SEM, you can have the best of both worlds.

Cheers,

Jim P.
}
} }
} } We have someone wanting to study bacterial attachment to sand grains.
} } Does anyone have any hints on ultramicrotomy of sand? This will
} be a first for us.
} }
} } We're going to start with negative staining, hoping to avoid
} cutting thins altogether, but if that doesn't work we thought we'd
} try adhering to poly-lysine coated Thermonox cover slips or
} embedding in agar before resin embedding.
} }
} } Thanks!
} }
} } Randy

--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 11-23, 2005, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2005


From MicroscopyL-request-at-ns.microscopy.com Sat Feb 26 10:39:49 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Sat, 26 Feb 2005 08:43:26 -0800 (PST)
Subject: [Microscopy] Re: Re: TEM--Ultramicrotomy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ok i think we need to know more about what you are
trying to do? are you attempting to see how the the
sand is attached to the bacteria? if that is the case
only thin sections will work. it is not impossible, we
cut collodial gold all the time with very few
problems, the issue is the size of the sand grains.
most sand grains are many times larger than bacteria.
the question is what are you trying to do. you could
for that matter if you are just attemping to provide a
substrat for the bacteria try polystyrene spheres.
they can be embedded and cut for TEM.
john
--- James Pawley {jbpawley-at-wisc.edu} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Hi Randy,
}
} If you don't have a good SEM, you might try to look
} at the bacterial
} film as a whole mount. Even at 100 kV you can see
} through a lot of
} tissue once the water is gone. Just put some sand on
} the grid and
} grow the bacteria on it, then remove, fix, CPD
} (perhaps a thin
} coating of carbon or metal, very thin) and view in
} the TEM. If you
} are careful, you should have clumps sticking off the
} side of the sand
} grains and some of these may show what you need. ANd
} if yo have a
} TEM/SEM, you can have the best of both worlds.
}
} Cheers,
}
} Jim P.
} }
} } }
} } } We have someone wanting to study bacterial
} attachment to sand grains.
} } } Does anyone have any hints on ultramicrotomy of
} sand? This will
} } be a first for us.
} } }
} } } We're going to start with negative staining,
} hoping to avoid
} } cutting thins altogether, but if that doesn't work
} we thought we'd
} } try adhering to poly-lysine coated Thermonox cover
} slips or
} } embedding in agar before resin embedding.
} } }
} } } Thanks!
} } }
} } } Randy
}
} --
}
} **********************************************
} Prof. James B. Pawley,
} Ph. 608-263-3147
} Room 223, Zoology Research Building,
} FAX 608-265-5315
} 1117 Johnson Ave., Madison, WI, 53706
} JBPAWLEY-at-WISC.EDU
} 3D Microscopy of Living Cells Course, June 11-23,
} 2005, UBC, Vancouver Canada
} Info: http://www.3dcourse.ubc.ca/ Applications
} due by March 15, 2005
}
}




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From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 08:46:16 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 28 Feb 2005 08:53:23 -0600
Subject: [Microscopy] Ultramicrotomy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Many thanks to everyone who has replied to my query on ultramicrotomy to
study bacterial attachment to sand grains. Lots of good ideas here. I
will go through all the replies in detail shortly and post a summary to
the list-server, if no one objects. We'll discuss these various
approaches with our customer and work this thing out.

Thanks again. You folks are great.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 10:47:10 2005



From: Roger Baker :      rcbaker-at-eden.infohwy.com
Date: Mon, 28 Feb 2005 10:51:28 -0600
Subject: [Microscopy] LED illumination for photomicroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I recently purchased a Chinese trinocular biological microscope for
general use. It was an overall good deal with practical design, good
optics, and flat fields and sharp focus out to the edge, although they
cut some corners on sturdyness of construction.

I also bought an inexpensive ($ 300) Chinese microscopy camera called
the Moticam 1000 with which I am not so happy because of a low
sensitivity CD chip and the fact that there is solarizing at high
illumination levels and the image pixelates when you use the full one
mega-pixel resolution. But I try to live with that. Considerably better
pictures are made by simply holding my 4 megapixel Canon Powershot A80
up to the eyepiece and shooting.

The high numerical aperture objectives means that the depth of focus is
small; this scope is solely designed to study flat transparent sections
mounted on slides. The built-in halogen illumination presents few
design problems because few photons are lost along the light path. One
bright white LED inserted in the same light path can nicely substitute
for their halogen bulb illuminator.

But I want to do reflectance illumination photography of flat-ground
sections of carbonate micro-fossils stained with gentian violet. Then I
put on a drop of glycerine and top with a coverslip. This makes the
denser structure of micro fossil details show up nicely against a deep
purple matrix, but it takes strong reflective illumination, especially
with my rather insensitive CCD camera.

I think the standard solution is an illuminator with a halogen light in
a box and two fiber optic bundles on stalks that can be arranged to
apply light to two sides of the field. This however costs as about as
much as my $750 microscope, so I have been investigating LEDS as an
inexpensive alternative.

What I have found is that you can use a ring of four white LEDs
(superbrightleds.com in Saint Louis) ) mounted on a fixture that clamps
on the objective. This works pretty nicely for visual work, since the
eye is quite sensitive at low light levels. And you can easily power
this gadget with 5 volts DC; each LED should its own 100 ohm current
limiting resistor.

But this is still not bright enough for my CCD cameras.

The best fix I have found for the sensitivity problem is to use a ring
of bright green LEDs.
I have four mounted now, but a ring of seven would still fit and would
be better. Each has to be independently adjusted by bending its leads
to cast its light onto the same small area under the objective. Since
the purple stain gives a monochromatic image anyhow, the resulting
image still shows up nicely under both visual examination and for
photography by my hand held digital camera, and even marginally for my
Moticam 1000.

Green LEDs are a definite improvement over white LEDs where my CCDs are
concerned.
Has LED illumination been discussed on this list before?

Are there any microscope cameras that can compete in both cost and
image quality with a consumer camera held up to the eyepiece? -- Roger,
Austin Tx



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 11:01:52 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Mon, 28 Feb 2005 12:09:39 -0500
Subject: [Microscopy] : New England Society for Microscopy (NESM) March Me

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The March meeting of the New England Society for Microscopy (NESM) will be held
on Tuesday, March 8th at the ATMC Conference Center, UMASS Dartmouth in Fall
River, MA. The meeting begins at 5pm with registration and a tour of the
MicroMagnetic Labs.

A buffet dinner follows at 6pm. At 6:45pm, the technicial presentations begin.
The first talk is "Electron Microscopy of Transparent Conducting Oxides" by
Prof. David Paine of Brown University and the second talk is "Using Inverse
Light Microscopy to Monitor Quantal Neurotransmitter Release in Real Time: The
End of a Neuroscience Dogma" by Dr. Emmanuel N. Pothos of Tufts University
School of Medicine.

Please contact Paul Bain, NESM Treasurer, by phone 617-432-3236 or by email:
paul_bain-at-hms.harvard.edu by Friday, March 4th to indicate if you are coming to
the meeting and dinner. (A head count is needed for the dinner). Payment will
be made at the door. Registration for members is $10.00 and $25.00 for
non-members. (Note: $15.00 for non-members goes towards a one-year membership
in NESM).

(Directions to UMASS Dartmouth can be found on NESM's (new) website:
http://nesm.cims.harvard.edu under Future Meetings.

We hope to see you there!

Peggy Sherwood
Corresponding Secretary, NESM









Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 11:20:22 2005



From: Ursula Potter :      U.J.Potter-at-bath.ac.uk
Date: Mon, 28 Feb 2005 17:27:27 +0000
Subject: [Microscopy] TEM section stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

Has anyone ever used Potassium Permanganate as a section stain? Apparently
it has been shown to improve the contrast in certain types of cell in the
artery wall and I wondered what might be going on in these cells that their
contrast should be so improved with this stain. Has anyone a method for the
staining?

Many thanks
Ursula
----------------

Ursula J. Potter
Centre for Electron Optical Studies (CEOS)
Building 3 West 2.15
The University of Bath
Claverton Down
Bath BA2 7AY
UK
Tel: 01225 385651
Email: U.J.Potter-at-bath.ac.uk


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 13:01:40 2005



From: John Raffensperger :      chiphead-at-sbcglobal.net
Date: Mon, 28 Feb 2005 11:08:08 -0800 (PST)
Subject: [Microscopy] Re: LED illumination for photomicroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roger;

You might find some past discussions, or care to raise
this issue again in the Yahoo Microscope group. Both
issues (LED Illumination and inexpensive camera
options) have been discussed there in the past. That
group is more focused (no pun intended) on LM. It is
also more of an amateur orientated group, although
many of the prime contributors are professionals (or
retirees). As a result of the audiance, a number of
low cost but "workable" alternatives are discussed.

The latest option for cameras seems to be using "Web
Cams", either out of the box (with standard occulars),
or by removing the lens assemblies and using a Photo
or Projection eyepiece.

I've played with LEDs myself, and have experienced
results similar to what you describe (OK, but not
spectacular). For me, it is more a matter of a light
source that can be used "in the field".

John Raffensperger, Jr.
Beaver Dam, Wisconsin

--- Roger Baker {rcbaker-at-eden.infohwy.com} wrote:
} Green LEDs are a definite improvement over white
} LEDs where my CCDs are
} concerned.
} Has LED illumination been discussed on this list
} before?
}
} Are there any microscope cameras that can compete in
} both cost and
} image quality with a consumer camera held up to the
} eyepiece? -- Roger,
} Austin Tx



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 15:02:54 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 28 Feb 2005 13:12:19 -0800
Subject: [Microscopy] Re: TEM section stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ursula
Potassium permanganate works in the similar way as OsO4 - it stains
membranes as far as know. For some unknown to me reason, it delivers very
good results with yeasts cells (used instead OsO4). I don't think it may
work on the sections because you basically don't have intact membranes (if
not fixed before embedding) after standard plastic embedding
procedure. From another hand, KMnO4 is quite strong oxidizer, so it may
"improve" section's staining by discoloring (oxidizing) something on the
surface of the section, so some particular structures may become visible
better. Have a great day, Sergey

At 05:27 PM 2/28/2005 +0000, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 16:08:38 2005



From: sghoshro-at-NMSU.Edu
Date: Mon, 28 Feb 2005 15:15:37 -0700 (MST)
Subject: [Microscopy] Asbestos analysis info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Everyone,

I am interested to know how and where to obtain training to perform TEM,
SEM, and light microscopic analysis of asbestos. Are there short courses
or certifications available to perform asbestos analysis ? Do we need any
acreditation to perform this sort of analysis ? If any one knows of good
references on asbestos analysis, books, protocols etc. then please let us
know. We will really appreciate your help.

Thanks in advance.

Soumitra

*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/


From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 18:07:26 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Mon, 28 Feb 2005 18:14:06 -0600
Subject: [Microscopy] Re: Asbestos analysis info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Soumitra,

I'd recommend that you contact John Reffner, currently at SensIR Technologies (JReffner-at-sensir.com). He is one of the founding fathers of the New Eng Association of Forensic Sciences (NEAFS) and, at one time, ran an asbestos analysis business with his wife, Sally. His other "partner in crime" is Tom Kubik, who ran a company I think was called "TAKA" (no further info). Both of them are experts in this field.

Also, there are special courses offered through the McCrone Institute in Chicago (www.mcri.org)

Hope this was helpful.

Best regards,
Barbara

Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
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Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). Visit www.MicroscopyEducation.com for details.

At 04:15 PM 2/28/2005, sghoshro-at-NMSU.Edu wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 19:16:14 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Mon, 28 Feb 2005 17:22:56 -0800 (PST)
Subject: [Microscopy] Re: TEM section stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i have used Potassium Permanganate as a TEM section
stain in conjuction with UA on corneas where we could
not use Pb citrate. it added some contrast to the
collagen. used a 1% solution and stained for 20
minutes at room temp. i can't give you any chemistry
of the stain. sorry about that.
john
--- Ursula Potter {U.J.Potter-at-bath.ac.uk} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Dear all,
}
} Has anyone ever used Potassium Permanganate as a
} section stain? Apparently
} it has been shown to improve the contrast in certain
} types of cell in the
} artery wall and I wondered what might be going on in
} these cells that their
} contrast should be so improved with this stain. Has
} anyone a method for the
} staining?
}
} Many thanks
} Ursula
} ----------------
}
} Ursula J. Potter
} Centre for Electron Optical Studies (CEOS)
} Building 3 West 2.15
} The University of Bath
} Claverton Down
} Bath BA2 7AY
} UK
} Tel: 01225 385651
} Email: U.J.Potter-at-bath.ac.uk
}
}


__________________________________________________
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Tired of spam? Yahoo! Mail has the best spam protection around
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From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 21:14:07 2005



From: WWAHL2-at-aol.com
Date: Mon, 28 Feb 2005 22:21:08 -0500
Subject: [Microscopy] superfine Sediment consolidate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello
Maybe somewhere out there can help with this.
What is the standard consolidate for finegrained sandstones in preparation before SEM so the sample won't leave any residue. I imagined a super fine cyanoacyrlate to consoildate but not coat.

--
Thanks, Bill Wahl

Winter
(785)628-4593 home... such as it is
(785)628-5715 office (machine)
Dept. of Geosciences
Fort Hays State University
Hays. Kans, 67601.

Summer
307-864-2997-or 2979
Wyoming Dinosaur Center
110 Carter Ranch Road
Thermopolis, Wyoming
82443

"a bowl of oatmeal tried to stare me down... and won."
John Prine

































































































































From MicroscopyL-request-at-ns.microscopy.com Mon Feb 28 23:51:53 2005



From: Rosemary White :      Rosemary.White-at-csiro.au
Date: Tue, 01 Mar 2005 16:59:58 +1100
Subject: [Microscopy] Re: Re: TEM section stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

FYI, making up stains in 9% isobutanol seems to improve contrast -
presumably it allows greater stain penetration. Seems to work for both
light and electron microscopy. Seemed also to improve immunogold label
without increasing background.

Roberts, IM (2002) Iso-butanol saturated water: a simple procedure for
increasing staining intensity of resin sections for light and electron
microscopy. J. Microscopy 207:97-107.

We're about to try this....
cheers,
Rosemary

} From: john hoffpauir {hoffpajo-at-yahoo.com}
} Date: Mon, 28 Feb 2005 17:22:56 -0800 (PST)
} To: Ursula Potter {U.J.Potter-at-bath.ac.uk} , Microscopy-at-microscopy.com
} Subject: [Microscopy] Re: TEM section stain
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} i have used Potassium Permanganate as a TEM section
} stain in conjuction with UA on corneas where we could
} not use Pb citrate. it added some contrast to the
} collagen. used a 1% solution and stained for 20
} minutes at room temp. i can't give you any chemistry
} of the stain. sorry about that.
} john
} --- Ursula Potter {U.J.Potter-at-bath.ac.uk} wrote:
}
} }
} }
} }
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} } Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
------------------------------------------------------------------------------}
-
} }
} } Dear all,
} }
} } Has anyone ever used Potassium Permanganate as a
} } section stain? Apparently
} } it has been shown to improve the contrast in certain
} } types of cell in the
} } artery wall and I wondered what might be going on in
} } these cells that their
} } contrast should be so improved with this stain. Has
} } anyone a method for the
} } staining?
} }
} } Many thanks
} } Ursula
} } ----------------
} }
} } Ursula J. Potter
} } Centre for Electron Optical Studies (CEOS)
} } Building 3 West 2.15
} } The University of Bath
} } Claverton Down
} } Bath BA2 7AY
} } UK
} } Tel: 01225 385651
} } Email: U.J.Potter-at-bath.ac.uk
} }
} }
}
}
} __________________________________________________
} Do You Yahoo!?
} Tired of spam? Yahoo! Mail has the best spam protection around
} http://mail.yahoo.com
}


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 07:06:19 2005



From: Richard Edelmann :      edelmare-at-MUOhio.edu
Date: Tue, 1 Mar 2005 08:12:22 -0500
Subject: [Microscopy] Re: TEM section stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ursula:


Several years ago I compared potassium permanganate post-section
staingin with barium post-section staining, I found barium staing to be much
cleaner to use and did an excellent job (superior to potassium permanganate
in my hands) on plant and fungal walls and protein/carbohydate matrices. I
can't put my hands on the procedure immediately but here the reference:


Hoch, H. C. 1977. Use of permanganate of increase the electron opacity

of fungal walls. Mycologia 69:1209-2113.


**************************************************************************

Harvey C. Hoch

Department of Plant Pathology

Barton Laboratory

Cornell University, NYSAES

Geneva, NY 14456-0462




{color} {param} 0100,0100,0100 {/param} { SEQ CHAPTER \h \r 1} {/color}

} Dear all,

{color} {param} 7F00,0000,0000 {/param} }

} Has anyone ever used Potassium Permanganate as a section stain? Apparently it

} has been shown to improve the contrast in certain types of cell in the artery

} wall and I wondered what might be going on in these cells that their contrast

} should be so improved with this stain. Has anyone a method for the staining?

}

} Many thanks

} Ursula

} ----------------

}

} Ursula J. Potter

} Centre for Electron Optical Studies (CEOS)

} Building 3 West 2.15

} The University of Bath

} Claverton Down

} Bath BA2 7AY

} UK

} Tel: 01225 385651

} Email: U.J.Potter-at-bath.ac.uk

}



{nofill}
Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 08:20:14 2005



From: Elaine F. Schumacher :      eschumacher-at-mccrone.com
Date: Tue, 1 Mar 2005 08:26:41 -0600
Subject: [Microscopy] Asbestos analysis info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As a follow-up to Barbara's reply, contact info for Thom Kubik is:

Thomas A. Kubik & Assoc. (TAKA)
P.O. Box 208
Greenlawn, NY 11704
Phone: 516-261-2117

Another contact is Peter M. Cooke:

Microscopy Instruction, Consultation & Analysis (MICA)
5807 N. Maplewood
Chicago, IL 60659
Phone: 773-334-2240
pmcooke-at-xsite.net

Regards,

Elaine

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************

-----Original Message-----
} From: sghoshro-at-NMSU.Edu [mailto:sghoshro-at-NMSU.Edu]
Sent: Monday, February 28, 2005 4:16 PM
To: MSA Listserve

Dear Everyone,

I am interested to know how and where to obtain training to perform TEM,

SEM, and light microscopic analysis of asbestos. Are there short courses

or certifications available to perform asbestos analysis ? Do we need
any
acreditation to perform this sort of analysis ? If any one knows of good

references on asbestos analysis, books, protocols etc. then please let
us
know. We will really appreciate your help.

Thanks in advance.

Soumitra

*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 09:16:42 2005



From: Randy Boltin :      rboltin-at-mvainc.com
Date: Tue, 01 Mar 2005 10:24:55 -0500
Subject: [Microscopy] Re: Asbestos analysis info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Soumitra:

McCrone Research Institute in Chicago (URL: www.mcri.org) and MICA (E-mail:
PMCOOKE-at-EARTHLINK.NET) are good sources for beginning courses in asbestos analysis
by polarized light microscopy (PLM) for bulk samples and by phase contrast
microscopy (PCM) for air samples by the NIOSH 7400 method. MVA Scientific
Consultants (the company I work with) offers a "Fundamentals of Asbestos Analysis
by Transmission Electron Microscopy" course at our facility in the Atlanta area.
You can check our website (www.mvainc.com) or call us at 770-662-8509 for more
information.

As for accreditation, the National Voluntary Laboratory Accreditation Program
(NVLAP) administered by the National Institute of Standards and Technology (NIST)
offers program-specific accreditations for asbestos analysis by PLM and by TEM
using the approved EPA methods. The NVLAP program for asbestos was mandated by the
Asbestos Hazards Emergency Response Act of 1986 for laboratories conducting
analysis for asbestos materials in school buildings. Lab accreditation is also
available through the American Association for Laboratory Accreditation (A2LA).
Both NVLAP and A2LA provide accreditation according to ISO17025 criteria. You may
want to note that much contract work these days requires that a lab be accredited.

Good reference material on the principles of bulk asbestos analysis by PLM is
available through MRI. EPA Test Method EPA/600/R-93/116: Method for the
Determination in Bulk Building Materials, is available from EPA. The TEM protocol
for analysis of asbestos in air samples is located in 40 CFR Part 763
(Asbestos-Containing Materials in Schools; Final Rule and Notice) Appendix A to
Subpart E.

I hope this helps. Good luck.

Best Regards,
Randy Boltin

sghoshro-at-NMSU.Edu wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Dear Everyone,
}
} I am interested to know how and where to obtain training to perform TEM,
} SEM, and light microscopic analysis of asbestos. Are there short courses
} or certifications available to perform asbestos analysis ? Do we need any
} acreditation to perform this sort of analysis ? If any one knows of good
} references on asbestos analysis, books, protocols etc. then please let us
} know. We will really appreciate your help.
}
} Thanks in advance.
}
} Soumitra
}
} *************************************************************
} Soumitra Ghoshroy
} College Associate Professor, Biology
} Director, Electron Microscopy Lab
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3268 (office), 646-3283 (lab)
} Fax: 505-646-3282
} e-mail:sghoshro-at-nmsu.edu
} http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
} http://emldata.nmsu.edu/eml/

--
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

The information in this email is confidential and may be legally privileged.
It is intended solely for the addressee. If you are not the intended recipient,
please delete the email and notify MVA Scientific Consultants of the transmission
error.
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From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 09:54:54 2005



From: David Patton :      David.Patton-at-uwe.ac.uk
Date: Tue, 01 Mar 2005 16:01:13 +0000
Subject: [Microscopy] TEM section stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When I started here in 1989 potassium permanganate was employed instead
of UA for safety reasons. I formed the impression from the literature
that UA would suit us better and discontinued use of permanganate.

We used it as either:
1% (aq) or 1% in phosphate buffer pH 6.5

It is supposed to be good for membranes but I did not do a rigourous
comparison.

Hayat (1989) (3rd Ed.) Principles and Techniques of Electron Microscopy.
Biological Applications covers it (pp280-281). Hayat describes using it
as part of a triple stain with permanganate and lead citrate.

Dave


-----Original Message-----
} From: Ursula Potter [mailto:U.J.Potter-at-bath.ac.uk]
Sent: 28 February 2005 17:27
To: Microscopy-at-microscopy.com

Dear all,

Has anyone ever used Potassium Permanganate as a section stain?
Apparently
it has been shown to improve the contrast in certain types of cell in
the
artery wall and I wondered what might be going on in these cells that
their
contrast should be so improved with this stain. Has anyone a method for
the
staining?

Many thanks
Ursula
----------------

Ursula J. Potter
Centre for Electron Optical Studies (CEOS)
Building 3 West 2.15
The University of Bath
Claverton Down
Bath BA2 7AY
UK
Tel: 01225 385651
Email: U.J.Potter-at-bath.ac.uk



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From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 09:57:37 2005



From: James Pawley :      jbpawley-at-wisc.edu
Date: Tue, 01 Mar 2005 10:04:29 -0600
Subject: [Microscopy] Re: LED illumination for photomicroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hi all,

I hope that it is not too commercial to tell you that, in the next
edition of The Handbook, there will be a fairly complete discussion
of how LEDs might be used in microscopy in the chapter on Non-laser
Light Sources by Andreas Nolte from Zeiss. At least it shows that the
idea has promise.

And on the cheap CCD front, I have had fair success hooking the
iSight camera from apple (~$140, 640x480, Firewire) to a Zeiss scope
by the simple expedient of removing the rubber "eye-glasses"
protector gasket on the normal 10x high-eyepoint occular and holding
the camera right in front of it with a plastic collar. It covers a
little more than the whole field of view (some black corners).
Although I haven't used it, I am told that one can use $30
"surveillance" software to record time-lapse sequences on a Mac using
this camera. Might be neat for non-microscope uses too.

Cheers,

Jim P.
--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 11-23, 2005, UBC, Vancouver Canada
Info: http:// www.3dcourse.ubc.ca/ Applications due by March 15, 2005


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 10:35:23 2005



From: Aleksandr Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Tue, 01 Mar 2005 16:45:14 +0000
Subject: [Microscopy] TEM: EM Atlas of tissues and organs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear collegues,

I need EM atlas of mammalian tissues and organs. What can you recommend?

--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 11:51:33 2005



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 01 Mar 2005 12:58:26 -0500
Subject: [Microscopy] Re: cheap CCD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A few months ago we posted, in response to a similar question, a link to
our CheapScopeTM at http://www.aecom.yu.edu/aif/gallery/cheapscope/index.htm

Here's an update:
http://cammer.net/blog/snowflake03.htm

-Michael


} And on the cheap CCD front, I have had fair success hooking the iSight
} camera from apple (~$140, 640x480, Firewire) to a Zeiss scope by the
} simple expedient of removing the rubber "eye-glasses" protector gasket on
} the normal 10x high-eyepoint occular and holding the camera right in
} front of it with a plastic collar.

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 11:53:34 2005



From: Mary Albrecht :      malbrecht-at-SSCI-INC.com
Date: Tue, 1 Mar 2005 13:00:47 -0500
Subject: [Microscopy] cGMP microscopy labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello

Are there any contract labs out there that analyse under cGMP conditions?
I'm specifically looking for analysing some pharmaceutical powders by SEM.

Thanks

Mary Albrecht
SSCI, Inc.


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 12:58:09 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Tue, 1 Mar 2005 11:04:46 -0800 (PST)
Subject: [Microscopy] Re: TEM: EM Atlas of tissues and organs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

fawcett had one years ago, not certain it is in print,
but should be in your library.
john

--- Aleksandr Mironov
{Aleksandr.Mironov-at-manchester.ac.uk} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Dear collegues,
}
} I need EM atlas of mammalian tissues and organs.
} What can you recommend?
}
} --
} Aleksandr Mironov
} Experimental Officer
} G450A, Stopford Building
} EM Unit, Faculty of Life Sciences
} University of Manchester
} Oxford Road
} Manchester
} M13 9PT
} UK
}
} Tel. +44-(0)161-275-7395
} Fax. +44-(0)161-275-5171
} E-mail: Aleksandr.Mironov-at-manchester.ac.uk
} MSN: amironov-at-hotmail.co.uk
} Web: http://www.empgu.man.ac.uk
}
}
}
}


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 13:37:19 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Tue, 01 Mar 2005 13:45:40 -0600
Subject: [Microscopy] Re: TEM: EM Atlas of tissues and organs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don Fawcett's "The Cell" has the finest TEM views of organelles and
membrane specializations. But is not really an atlas of tissues and
organs. Kessel and Kardon is the best SEM atlas I know.


At 10:45 AM 03/01/05, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 15:55:07 2005



From: William P. Sharp :      wsharp-at-asu.edu
Date: Tue, 01 Mar 2005 15:01:55 -0700
Subject: [Microscopy] Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Microscopy Listers -

Our perennial lament - does anyone out there know of a University or
Institute (non-profit) that operates an EM facility or a Bioimaging
facility that takes in enough money through charge back of users to cover
most or all of the costs of lab operation, to include service contracts on
major instruments? If so, may we know the rates charged? Are there a
relatively large number of outside users that are billed at a much higher
rate than inside users?

We at ASU are in the second year of ramping up the rates charged for zero
to an average charge rate over four years and are feeling some pressure to
charge enough to pay for all operations of our bioimaging facilities, EM
and light as soon as possible. Those of us who are responsible for the
facilities are concerned about pricing ourselves out of the market and we
need some idea of whether it can be done without a total upheaval of
service or the loss of our core facilities.

Thanks for any information or advice you may have for us -

Bill Sharp

William P. Sharp
Arizona State University
School of Life Sciences, box 4501
Tempe, AZ 85287-4501
Phone - (480)-965-3210
Fax - (480)-965-6899



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 16:02:17 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 01 Mar 2005 17:08:40 -0500
Subject: [Microscopy] Re: TEM: EM Atlas of tissues and organs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My all-time favorite for this is Johannes A G Rhodin's "Histology:A
Text and Atlas" copyright 1974 Oxford Univ. Press.
It may be out of print, but your library should have it. You may be
able to scare one up on Amazon.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 20:13:59 2005



From: Anthony Garratt-Reed :      tonygr-at-MIT.EDU
Date: Tue, 01 Mar 2005 21:19:31 -0500
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bill-

We cover 82%. We are principally a Materials facility, and are a lot more
than imaging (you can see what we have at http://prism.mit.edu).

The trick is that you have to pare down on the service you provide your
users, and you can't be paranoid about them making mistakes. Virtually all
use is "self use" where the users are operating the instruments.

As one example, we operate 4 200KV TEM's (one with EDX), a VG STEM, and 3
SEM's of varying capability (all with EDX, one with EBSD and ESEM),
together with 2 ion mills, a cryomicrotome and a host of other ancilliary
equipment, with 2.4 FTE's. All EM's except the VG are under manufacturer's
contract.

The staff give users very basic operating instructions, then let them
go. We spend most of our time fixing mistakes, telling users that the
instrument won't work because they haven't turned it on, and in similar
pursuits, and we rely on our service contracts to bring in technicians to
fix broken things. You'd be surprised how often we have to explain that an
image is not in focus if it doesn't look sharp!

There are downsides to this philosophy, of course. However, it has a
number of major benefits: the users are forced to learn (and hopefully
understand) the techniques they are employing (after all, we are a
University!); surprisingly, productivity seems to be much higher than when
we were more circumspect; and, of course, we can operate within a
manageable budget (our fiscal director would like the subsidy to be zero,
but we seem to have come to a workable equilibrium - at least for the
present - with the 18%).

Users do break things, but we find that we lose more productivity by
limiting their access than we do by fixing the mistakes. Since our raison
d'etre is to generate results, we opt for the productivity.

One perspective. I hope it helps.

Tony Garratt-Reed.


At 03:01 PM 3/1/2005 -0700, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*********************************************
Anthony J. Garratt-Reed, M.A., D.Phil
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
*********************************************
Phone: (617) 253-4622
Fax: (617) 258-6479
*********************************************



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 1 20:48:10 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 01 Mar 2005 18:55:52 -0800
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

William
In my opinion, it's impossible to make EM facility profitable or
self-sustained. It was discussed at ListServer many times and the
conclusion was that most EM facilities need external funding to survive.
What we usually recover from the users is 30-50% at the best. At least,
this is my experience and opinion. Sergey

At 02:01 PM 3/1/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 04:56:13 2005



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 2 Mar 2005 11:59:45 +0100
Subject: [Microscopy] Goldstein "Scanning elctron microscopy" Quest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

Having the 1980th edition of Godstein et al. Scanning electron microscopy
book, I wanted to buy the new one, and I am waiting since mid November.
The bookseller says me the book would be in reprint. But on the other
side, people from Springeronline saiy they have in on stock (first print
?)... With the confidence we can have on web site informations (amzon or
editors).

I asked me if someone has soon seen the 2005 reprint of that book, or if
it is still being printed.

Thanks

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 05:11:30 2005



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 2 Mar 2005 12:14:30 +0100
Subject: [Microscopy] Re: LED illumination for photomicroscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Have a look at

http://www.microscopy-uk.org.uk/mag/artaug03/jwled.html

Some informations about white LED illumination. From my experience, it's
not easy to focus a lot of LEDs on the same place. But it's very
interseting for field instruments, working with NiCd cells.


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Mon, 28 Feb 2005, Roger Baker wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} I recently purchased a Chinese trinocular biological microscope for
} general use. It was an overall good deal with practical design, good
} optics, and flat fields and sharp focus out to the edge, although they
} cut some corners on sturdyness of construction.
}
} I also bought an inexpensive ($ 300) Chinese microscopy camera called
} the Moticam 1000 with which I am not so happy because of a low
} sensitivity CD chip and the fact that there is solarizing at high
} illumination levels and the image pixelates when you use the full one
} mega-pixel resolution. But I try to live with that. Considerably better
} pictures are made by simply holding my 4 megapixel Canon Powershot A80
} up to the eyepiece and shooting.
}
} The high numerical aperture objectives means that the depth of focus is
} small; this scope is solely designed to study flat transparent sections
} mounted on slides. The built-in halogen illumination presents few
} design problems because few photons are lost along the light path. One
} bright white LED inserted in the same light path can nicely substitute
} for their halogen bulb illuminator.
}
} But I want to do reflectance illumination photography of flat-ground
} sections of carbonate micro-fossils stained with gentian violet. Then I
} put on a drop of glycerine and top with a coverslip. This makes the
} denser structure of micro fossil details show up nicely against a deep
} purple matrix, but it takes strong reflective illumination, especially
} with my rather insensitive CCD camera.
}
} I think the standard solution is an illuminator with a halogen light in
} a box and two fiber optic bundles on stalks that can be arranged to
} apply light to two sides of the field. This however costs as about as
} much as my $750 microscope, so I have been investigating LEDS as an
} inexpensive alternative.
}
} What I have found is that you can use a ring of four white LEDs
} (superbrightleds.com in Saint Louis) ) mounted on a fixture that clamps
} on the objective. This works pretty nicely for visual work, since the
} eye is quite sensitive at low light levels. And you can easily power
} this gadget with 5 volts DC; each LED should its own 100 ohm current
} limiting resistor.
}
} But this is still not bright enough for my CCD cameras.
}
} The best fix I have found for the sensitivity problem is to use a ring
} of bright green LEDs.
} I have four mounted now, but a ring of seven would still fit and would
} be better. Each has to be independently adjusted by bending its leads
} to cast its light onto the same small area under the objective. Since
} the purple stain gives a monochromatic image anyhow, the resulting
} image still shows up nicely under both visual examination and for
} photography by my hand held digital camera, and even marginally for my
} Moticam 1000.
}
} Green LEDs are a definite improvement over white LEDs where my CCDs are
} concerned.
} Has LED illumination been discussed on this list before?
}
} Are there any microscope cameras that can compete in both cost and
} image quality with a consumer camera held up to the eyepiece? -- Roger,
} Austin Tx
}
}




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 06:33:22 2005



From: Basil Julies :      bjulies-at-uwc.ac.za
Date: Wed, 02 Mar 2005 14:42:17 +0200
Subject: [Microscopy] Hitachi 650 SEM and 800 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everybody

Do you know of anyone who has a broken Hitachi 650 SEM or 800 TEM? I'm
contacting you all the way from South Africa. We cannot afford new
instruments and desperately need to keep the existing ones alive by
using spare parts from other similar obsolete instruments. These
instruments are more than 20 years old and some of the electronic
components are no longer available.
This is a desperate plea for help. Even though money is very scarce on
our side, we are nevertheless willing to pay something towards these
spare parts.

Urgently awaiting a reply

Yours sincerely

Basil Julies





Dr. Basil Julies
Director
Electron Microscope Unit
Physics Department
University of the Western Cape
Private Bag X17
Bellville 7535 South Africa
Tel : (27)(21) 959 2327 or 959 3458
Fax : (27)(21) 959 1335 or 959 3474


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 09:45:42 2005



From: Anita Garg :      Anita.Garg-at-grc.nasa.gov
Date: Wed, 02 Mar 2005 10:52:23 -0500
Subject: [Microscopy] Etching NiTiPt alloys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All
Does anybody have experience in etching NiTiPt alloys (~ 25at.%Pt)to reveal
martensite and/or grain boundaries? Any help would be highly appreciated.
TIA
Anita



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 10:14:43 2005



From: Evgenia Pekarskaya :      pekarskaya-at-mail.pse.umass.edu
Date: Wed, 2 Mar 2005 11:21:40 -0500
Subject: [Microscopy] Goldstein "Scanning elctron microscopy" Quest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jacques,

I have the third edition of "Scanning Electron Microscopy and X-ray
Microanalysis" by Goldstein et al. published in 2003 by Kluwer Academic
(ISBN: 0-306-47292-9). I believe that this is the latest edition.

Evgenia Pekarskaya

========================
Evgenia Pekarskaya
Keck Electron Microscopy Laboratory
Polymer Science & Engineering
UMass, Amherst; Conte A229
Tel. 413 545 2261
E-fax 325 202 7338
http://www.umassmicroscopy.com/
========================

-----Original Message-----
} From: Faerber Jacques [mailto:Jacques.Faerber-at-ipcms.u-strasbg.fr]
Sent: Wednesday, March 02, 2005 6:00 AM
To: Microscopy Society of America

Hi all

Having the 1980th edition of Godstein et al. Scanning electron microscopy
book, I wanted to buy the new one, and I am waiting since mid November.
The bookseller says me the book would be in reprint. But on the other
side, people from Springeronline saiy they have in on stock (first print
?)... With the confidence we can have on web site informations (amzon or
editors).

I asked me if someone has soon seen the 2005 reprint of that book, or if
it is still being printed.

Thanks

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr








From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 10:37:56 2005



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Wed, 02 Mar 2005 11:44:45 -0500
Subject: [Microscopy] re: cheap ccd

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For those of you who aren't completely sick of this discussion...

SPIE oemagazine March 2005 p. 28 is an article by Lionel Baker
lionelbaker-at-ntlworld.com who gives technical details on mounting one ocular
of binoculars to a consumer digital camera to produce 24X telescopic
optical zoom.

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 10:50:21 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Wed, 02 Mar 2005 11:51:18 -0500
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Bill,

We are all struggling with the same problem!
We have had to raise charges here too, yet are
only recovering about 60-70% of our cost at the
moment. And that does not include some salaries
that are covered by external grants. If these are
put into the equation, then we are recovering less
than 50%.
Doubling the charges is not an option, as we are
already losing some users due to our current charges.
Only 3 solutions that I can see:
- Boost productivity, but that requires some investment
in new, more sophisticated instrumentation (such as
new CCD camera for the TEM, etc);
- Rely more on NIH, PPG grants, etc, to cover facility
staff salaries;
- Some forms of subsidies from the university. An
important argument is that a good imaging facility
greatly enhances the ability of a university to attract
top faculty, and help faculty get more external
funding, a good portion of which goes back to the
university in the form of overheads! So investing in
a facility should make sense, although I realize that
your typical administrative office might be quite
unmoved by that king of logic!

Good luck

Marc


On Tuesday, March 1, 2005, at 05:01 PM, William P. Sharp wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Microscopy Listers -
}
} Our perennial lament - does anyone out there know of a University or
} Institute (non-profit) that operates an EM facility or a Bioimaging
} facility that takes in enough money through charge back of users to
} cover most or all of the costs of lab operation, to include service
} contracts on major instruments? If so, may we know the rates charged?
} Are there a relatively large number of outside users that are billed
} at a much higher rate than inside users?
}
} We at ASU are in the second year of ramping up the rates charged for
} zero to an average charge rate over four years and are feeling some
} pressure to charge enough to pay for all operations of our bioimaging
} facilities, EM and light as soon as possible. Those of us who are
} responsible for the facilities are concerned about pricing ourselves
} out of the market and we need some idea of whether it can be done
} without a total upheaval of service or the loss of our core } facilities.
}
} Thanks for any information or advice you may have for us -
}
} Bill Sharp
}
} William P. Sharp
} Arizona State University
} School of Life Sciences, box 4501
} Tempe, AZ 85287-4501
} Phone - (480)-965-3210
} Fax - (480)-965-6899
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 14:24:50 2005



From: sghoshro-at-NMSU.Edu
Date: Wed, 2 Mar 2005 13:31:45 -0700 (MST)
Subject: [Microscopy] Thank you for asbestos info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all of you who responded to my asbestos query. It was indeed a
big help and very informative. Thanks for taking the time to respond and
we will get in touch with some people as suggested by some of you.

This group is just wonderful.

Best wishes,

Soumitra


*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 14:48:22 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 02 Mar 2005 15:54:59 -0500
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bill,
We have run into the same problem. After years of having EVERYTHING
covered we are under increased pressure to cover everything. This has
gotten worse as University budgets have shrunk. We cover service contracts
for 3 microscopes (~$60,000) and additional for a full service and
multi-user facility with TEM, SEM, and LM. We still fall about 15-20% short
of covering everything (including data and telephone lines, postage, all
consumables, etc).

The administration's response is to increase rates. I expect increasing
rates will result in decreased user hours and no gain in revenue...until you
get to the point where users drop off and revenue decreases. Unfortunately
non-microscopists do not understand that this is not a speed discipline. It
takes time to adequately align a scope and thoroughly examine samples. If a
user is concerned about watching the clock than quality of research is sure
to suffer. Aren't the facilities here to assist in generating good
research? Or maybe I have been mistaken about this all these years. With
hundreds of thousands of dollars invested in instrumentation, you would
think that universities could find 10-20 thousand each year to help keep
them accessible to the maximum number of researchers.

Debby

P.S. I am saving responses to Bill's E-mail and intend to give them all to
my administrators and advisory committee. I think they can do a lot to help
support my arguments so keep them coming!

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 3/1/05 5:01 PM, "William P. Sharp" {wsharp-at-asu.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Microscopy Listers -
}
} Our perennial lament - does anyone out there know of a University or
} Institute (non-profit) that operates an EM facility or a Bioimaging
} facility that takes in enough money through charge back of users to cover
} most or all of the costs of lab operation, to include service contracts on
} major instruments? If so, may we know the rates charged? Are there a
} relatively large number of outside users that are billed at a much higher
} rate than inside users?
}
} We at ASU are in the second year of ramping up the rates charged for zero
} to an average charge rate over four years and are feeling some pressure to
} charge enough to pay for all operations of our bioimaging facilities, EM
} and light as soon as possible. Those of us who are responsible for the
} facilities are concerned about pricing ourselves out of the market and we
} need some idea of whether it can be done without a total upheaval of
} service or the loss of our core facilities.
}
} Thanks for any information or advice you may have for us -
}
} Bill Sharp
}
} William P. Sharp
} Arizona State University
} School of Life Sciences, box 4501
} Tempe, AZ 85287-4501
} Phone - (480)-965-3210
} Fax - (480)-965-6899
}
}




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 16:47:42 2005



From: John Knowles :      john-at-microvisionlabs.com
Date: Wed, 2 Mar 2005 17:49:38 -0500
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bill et al,

I own and operate a small analytical microscopy lab and like everyone
else in the dreaded private sector, I have to make a profit every month
or else I do not get paid. Because of this my focus is naturally on
serving the client's microscopy needs and not so much on research.

I was always under the impression that Universities were mainly focused
on research. When part of my tax dollars goes to pay for new equipment
for University microscopy labs I am ok with it as long as I believe that
it is going to be used for education and/or research projects that the
University is doing. When I hear that this equipment is being used to
set up microscopy service labs that are competing against me then I am
definitely not ok with it. Why should I have to pay for all of my
equipment, come in on weekends and stay late into the night doing my own
equipment maintenance and service, and pay 100% of my costs when the
government is using my tax money to help my competition?

There are a number of local Universities in the Boston area that are
happy to accept government grants for new equipment and then use it to
take business away from privately owned labs like mine. I have talked
to many companies that have told me that they go to a University
Microscopy lab to get there work done instead of a lab like mine because
the Universities rates are so low. If I only had to cover 80% (or less)
of my costs and got grants for my equipment I could drop my rates as
well. MIT is one of the few Universities that play by the rules. They
do not take business away from privately own labs like mine. In fact
they send private companies away and recommend labs like mine to them.
And for that I am grateful.

I do not think Universities should compete against private labs when
they are getting government money (just in case you have not figured
that out by now 8^). If they must then at least raise your rates to the
typical rates in your area. That would make it a fair playing field.

Another thought I had, do your administrators factor in any of the
students tuition when calculating your profitability? There are many
students that pick which college they are going to attend based on, at
least in part, what the facilities are at that location. A microscopy
department is a big asset to a school that is trying to attract students
interested in the sciences (e.g. biology and materials). When you look
at a University Microscopy Dept. as a part of the whole education system
then it is silly to think it has to act like a business. The campus
grounds need to be kept up as well in order for a university to attract
and keep students. Does your administration ask the grounds crew to be
profitable as well? Maybe compete with locate landscaping companies
mowing lawns and trimming bushes. I bet not.

John Knowles
President
MicroVision Laboratories, Inc.


-----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: Wednesday, March 02, 2005 3:55 PM
To: William P. Sharp; Microscopy-at-microscopy.com

Bill,
We have run into the same problem. After years of having EVERYTHING
covered we are under increased pressure to cover everything. This has
gotten worse as University budgets have shrunk. We cover service
contracts
for 3 microscopes (~$60,000) and additional for a full service and
multi-user facility with TEM, SEM, and LM. We still fall about 15-20%
short
of covering everything (including data and telephone lines, postage, all
consumables, etc).

The administration's response is to increase rates. I expect
increasing
rates will result in decreased user hours and no gain in revenue...until
you
get to the point where users drop off and revenue decreases.
Unfortunately
non-microscopists do not understand that this is not a speed discipline.
It
takes time to adequately align a scope and thoroughly examine samples.
If a
user is concerned about watching the clock than quality of research is
sure
to suffer. Aren't the facilities here to assist in generating good
research? Or maybe I have been mistaken about this all these years.
With
hundreds of thousands of dollars invested in instrumentation, you would
think that universities could find 10-20 thousand each year to help keep
them accessible to the maximum number of researchers.

Debby

P.S. I am saving responses to Bill's E-mail and intend to give them all
to
my administrators and advisory committee. I think they can do a lot to
help
support my arguments so keep them coming!

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 3/1/05 5:01 PM, "William P. Sharp" {wsharp-at-asu.edu} wrote:

}
}
}
------------------------------------------------------------------------
------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
------}
-
}
} Microscopy Listers -
}
} Our perennial lament - does anyone out there know of a University or
} Institute (non-profit) that operates an EM facility or a Bioimaging
} facility that takes in enough money through charge back of users to
cover
} most or all of the costs of lab operation, to include service
contracts on
} major instruments? If so, may we know the rates charged? Are there a
} relatively large number of outside users that are billed at a much
higher
} rate than inside users?
}
} We at ASU are in the second year of ramping up the rates charged for
zero
} to an average charge rate over four years and are feeling some
pressure to
} charge enough to pay for all operations of our bioimaging facilities,
EM
} and light as soon as possible. Those of us who are responsible for the
} facilities are concerned about pricing ourselves out of the market and
we
} need some idea of whether it can be done without a total upheaval of
} service or the loss of our core facilities.
}
} Thanks for any information or advice you may have for us -
}
} Bill Sharp
}
} William P. Sharp
} Arizona State University
} School of Life Sciences, box 4501
} Tempe, AZ 85287-4501
} Phone - (480)-965-3210
} Fax - (480)-965-6899
}
}




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 17:10:34 2005



From: George Langford, Sc.D. :      amenex-at-amenex.com
Date: Wed, 2 Mar 2005 18:17:33 -0500 (EST)
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Microscopists !

It used to be that my hackles would rise whenever this sort of topic
started. However, in the present instance the discussion has been
healthy, albeit depressing. When this starts at the research facilities
of major companies, one knows that big layoffs are certain to follow,
because the lab is now considered expendable ...

While my company (Amenex Associates) was going strong, three of
us managed to keep an ETEC Autoprobe busy, along with associated
metallographic imaging and specimen preparation facilities, located
in about 3000 square feet of office space. We had enough consulting
business to make living wages for about fifteen years. Then the
landlord wanted more money, a longer lease, and separate utilities
billing. That was it. Now my business partner and I work out of our
homes. We send our lab work to a small private lab which seems also
to be hanging on with even a little left over to buy new equipment.

The real problem is with rising inefficiency at the management level,
the same force that's causing college tuition to rise astronomically.
I remember the ridiculous overhead rates - even overhead on tuition
payments for my graduate students - such that an umpteen dollar
research contract yielded mebbe a thousand dollars a year for new
equipment or consumables.

George Langford, Sc.D.
Principal Consultant
Amenex Associates, Inc.
amenex-at-amenex.com
http://www.amenex.com/


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 18:27:46 2005



From: Alan Stone :      as-at-astonmet.com
Date: Wed, 02 Mar 2005 18:34:18 -0600
Subject: [Microscopy] Re: RE: Re: Cost Recovery in Imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

I also have my own small consulting lab and face the same issues. There
are others in the group who know about this, but I believe that NSF grants
include a clause taking in outside work not be at the expense of local
commercial facilities. We are a small community and we should expect our
academic peers to honor the terms of their grant money.

I would suggest that you ensure that the local academics are fully aware of
your capabilities so they have the excuse that they are unaware of your
existence is not valid.

Alan Stone
ASTON Metallurgical Services Co., Inc.


At 04:49 PM 3/2/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 18:50:08 2005



From: Bryony James :      b.james-at-auckland.ac.nz
Date: Thu, 03 Mar 2005 13:57:02 +1300
Subject: [Microscopy] Re: Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This debate seems to crop up with a worrying increase in frequency. The
argument that good research and good researchers require access to
state-of-the-art facilities goes without saying. The only people who
can't seem to see this are University administrators and those who reply
"surely good research brings in money to pay user charges?" (where do
you even start with that question?).

In my case I run a facility that is tiny by US standards but fully
tooled up by the standards of New Zealand (1 FEG-SEM +EDS +cryo, 1
FEG-ESEM +EDS +EBSD, 1 imaging XPS and 1 AFM). I think we are almost
unique in that the University expects us to cover not only salaries,
consumables and maintenance costs but all our depreciation as well.
Each year I have come up with a new financial "model" that includes
direct contributions from the Faculties in order to break even. This
year I have been told that is not acceptable and user charges have to
cover everything. It's going to be an interesting year, anyone want to
buy a kidney?

I would like to ask the listserv has anyone ever done (or seen done) the
financial analyis of what one published paper is worth to a tertiary
institution? Obviously the answer will change with the funding
situation in each country but it would be an interesting comparison.

Cheers
Bryony James

Research Centre for Surface and Materials Science
University of Auckland
New Zealand


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 19:01:49 2005



From: Hicks, Aaron :      A.W.Hicks-at-massey.ac.nz
Date: Thu, 3 Mar 2005 14:08:40 +1300
Subject: [Microscopy] Re: RE: Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I've been following this thread with some interest.

Is the situation similar for other facilities in other countries?

Aaron Hicks
Electron Microscopy Preparation Technician

Comparative Physiology and Anatomy
Institute of Veterinary, Animal, and Biomedical Sciences
Massey University

PN-412
Private Bag 11 222
Palmerston North
New Zealand

Phone +64 06 350 4874


-----Original Message-----
} From: Alan Stone [mailto:as-at-astonmet.com]
Sent: Thursday, 3 March 2005 1:34 p.m.
To: John Knowles
Cc: microscopy-at-microscopy.com


John,

I also have my own small consulting lab and face the same issues. There

are others in the group who know about this, but I believe that NSF
grants
include a clause taking in outside work not be at the expense of local
commercial facilities. We are a small community and we should expect our

academic peers to honor the terms of their grant money.

I would suggest that you ensure that the local academics are fully aware
of
your capabilities so they have the excuse that they are unaware of your
existence is not valid.

Alan Stone
ASTON Metallurgical Services Co., Inc.


At 04:49 PM 3/2/2005, you wrote:


} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} or else I do not get paid. Because of this my focus is naturally on
} serving the client's microscopy needs and not so much on research.
}
} I was always under the impression that Universities were mainly focused

} on research. When part of my tax dollars goes to pay for new equipment

} for University microscopy labs I am ok with it as long as I believe
} that it is going to be used for education and/or research projects that

} the University is doing. When I hear that this equipment is being used

} to set up microscopy service labs that are competing against me then I
} am definitely not ok with it. Why should I have to pay for all of my
} equipment, come in on weekends and stay late into the night doing my
} own equipment maintenance and service, and pay 100% of my costs when
} the government is using my tax money to help my competition?
}
} There are a number of local Universities in the Boston area that are
} happy to accept government grants for new equipment and then use it to
} take business away from privately owned labs like mine. I have talked
} to many companies that have told me that they go to a University
} Microscopy lab to get there work done instead of a lab like mine
} because the Universities rates are so low. If I only had to cover 80%
} (or less) of my costs and got grants for my equipment I could drop my
} rates as well. MIT is one of the few Universities that play by the
} rules. They do not take business away from privately own labs like
} mine. In fact they send private companies away and recommend labs like

} mine to them. And for that I am grateful.
}
} I do not think Universities should compete against private labs when
} they are getting government money (just in case you have not figured
} that out by now 8^). If they must then at least raise your rates to
} the typical rates in your area. That would make it a fair playing
} field.
}
} Another thought I had, do your administrators factor in any of the
} students tuition when calculating your profitability? There are many
} students that pick which college they are going to attend based on, at
} least in part, what the facilities are at that location. A microscopy
} department is a big asset to a school that is trying to attract
} students interested in the sciences (e.g. biology and materials). When

} you look at a University Microscopy Dept. as a part of the whole
} education system then it is silly to think it has to act like a
} business. The campus grounds need to be kept up as well in order for a

} university to attract and keep students. Does your administration ask
} the grounds crew to be profitable as well? Maybe compete with locate
} landscaping companies mowing lawns and trimming bushes. I bet not.
}
} John Knowles
} President
} MicroVision Laboratories, Inc.
}
}
} -----Original Message-----
} } From: Debby Sherman [mailto:dsherman-at-purdue.edu]
} Sent: Wednesday, March 02, 2005 3:55 PM
} To: William P. Sharp; Microscopy-at-microscopy.com
} Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities
}
}
}
} -----------------------------------------------------------------------
} -
} ------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} This has gotten worse as University budgets have shrunk. We cover
} service contracts for 3 microscopes (~$60,000) and additional for a
} full service and multi-user facility with TEM, SEM, and LM. We still
} fall about 15-20% short
} of covering everything (including data and telephone lines, postage,
all
} consumables, etc).
}
} The administration's response is to increase rates. I expect
} increasing rates will result in decreased user hours and no gain in
} revenue...until you
} get to the point where users drop off and revenue decreases.
} Unfortunately
} non-microscopists do not understand that this is not a speed
discipline.
} It
} takes time to adequately align a scope and thoroughly examine samples.
} If a
} user is concerned about watching the clock than quality of research is
} sure
} to suffer. Aren't the facilities here to assist in generating good
} research? Or maybe I have been mistaken about this all these years.
} With
} hundreds of thousands of dollars invested in instrumentation, you would
} think that universities could find 10-20 thousand each year to help
keep
} them accessible to the maximum number of researchers.
}
} Debby
}
} P.S. I am saving responses to Bill's E-mail and intend to give them
} all to my administrators and advisory committee. I think they can do a

} lot to help
} support my arguments so keep them coming!
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
}
}
} On 3/1/05 5:01 PM, "William P. Sharp" {wsharp-at-asu.edu} wrote:
}
} }
} }
} }
} -----------------------------------------------------------------------
} -
} ------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------
} -
} ------}
} -
} }
} } Microscopy Listers -
} }
} } Our perennial lament - does anyone out there know of a University or

} } Institute (non-profit) that operates an EM facility or a Bioimaging
} } facility that takes in enough money through charge back of users to
} cover
} } most or all of the costs of lab operation, to include service
} contracts on
} } major instruments? If so, may we know the rates charged? Are there a

} } relatively large number of outside users that are billed at a much
} higher
} } rate than inside users?
} }
} } We at ASU are in the second year of ramping up the rates charged for
} zero
} } to an average charge rate over four years and are feeling some
} pressure to
} } charge enough to pay for all operations of our bioimaging
} } facilities,
} EM
} } and light as soon as possible. Those of us who are responsible for
} } the facilities are concerned about pricing ourselves out of the
} } market and
} we
} } need some idea of whether it can be done without a total upheaval of

} } service or the loss of our core facilities.
} }
} } Thanks for any information or advice you may have for us -
} }
} } Bill Sharp
} }
} } William P. Sharp
} } Arizona State University
} } School of Life Sciences, box 4501
} } Tempe, AZ 85287-4501
} } Phone - (480)-965-3210
} } Fax - (480)-965-6899
} }
} }





From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 19:13:57 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Wed, 02 Mar 2005 19:26:17 -0600
Subject: [Microscopy] Re: Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

debbie

if it will help, i have been filing these notes for the past several
years. it won't be fancy, but i can share them all. also, i believe
phil oshel did something on this as a survey 2-3 years ago.

paul



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 19:38:36 2005



From: cosandey-at-scils.rutgers.edu (by way of MicroscopyListserver)
Date: Wed, 2 Mar 2005 19:45:57 -0600
Subject: [Microscopy] viaWWW: Research Associate Position in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cosandey-at-scils.rutgers.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, March 2, 2005 at 18:59:31
---------------------------------------------------------------------------

Email: cosandey-at-scils.rutgers.edu
Name: Fred Cosandey

Organization: Rutgers University

Title-Subject: [Microscopy] [Filtered] MListserver: Research Associate Position in TEM

Question: Research Associate Position
Transmission Electron Microscopy

The Department of Ceramic and Materials Engineering at Rutgers University is currently seeking a candidate for a Research Associate position in Transmission Electron Microscopy.
The successful candidate should have a PhD degree in Materials Science or related discipline and have extensive experience in the use of transmission electron microscope including HAADF, GIF and EELS spectroscopy techniques. Prior experience with JEOL 2010F STEM would be preferable.
Specific projects involve EELS analysis of nanostructured electrode materials used in Li-Ion batteries, including EELS determination of valence state and, structural and chemical analysis of electrodes during Li-induced phase transformations. Also, chemical and electronic structure determination of interfaces in oxides and metal-oxides systems using HAADF STEM and EELS.

The appointment will be for one year and may be renewable for subsequent years. Interested candidate should send their CV with three references to:

Professor Frederic Cosandey
Department of Ceramic and Materials Engineering
Rutgers University
607 Taylor Rd.
Piscataway, NJ 08854-8065,
cosandey-at-rscils.rutgers.edu, 732-445-4942 (phone), 732-445-5977 (fax)

Rutgers University is an equal opportunity/affirmative action employer.


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 2 21:41:20 2005



From: Rosemary White :      Rosemary.White-at-csiro.au
Date: Thu, 03 Mar 2005 14:48:57 +1100
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

Ah yes, the same old chestnut keeps coming from administrators.
My response to this was as follows.

Because we're a small unit (TEM, SEM, confocal, other LMs, 2 staff) I can
keep pretty good track of all the work going through. I keep very good
track of the publications that come out using work done in Microscopy.

Now, our Division of CSIRO - Plant Industry - has an income of about $70
million per year (about 60% federal, 40% industry). The cost of maintaining
Microscopy is about $370,000 per year (I'm expected to recover two salaries,
depreciation on equipment - which is about half the total, consumables,
rental on the building, all utilities, etc. - the lot). Of the publications
from the Division of Plant Industry, at least 10% use Microscopy to some
degree. I worked out that, on average, Microscopy's contribution to these
publications was about 2% per year, minimum - between 5% and 80% of each of
those 10% of publications. Now 2% of $70 million is about $1.4 million that
Microscopy might expect as input to support this output - i.e. just under 4
times our actual cost.

QED - we are way overperforming, so how can it be argued that we cost too
much? (Yes, it's pretty rough, and of course, I haven't included the salary
time others have spent in Microscopy using the equipment....) In our case,
it's an accounting exercise - our costs should be associated with a project
number rather being "general costs", but the problem is, if we increase our
charges to internal users, our external, publicly-funded collaborators will
find us way too expensive to work with.

I suspect that the need to account for an expense against some specific
account number may underlie some of these demands for full cost recovery.
Our accountants hate having expenses that they can't allocate to a
particular grant or project number. Because we (Microscopy) recover only
about 50% of costs directly from users, it means that the accountants have
to somehow spread the rest of the costs across all the other numbers they've
got, and they don't like doing that (although they're quite happy to do it
for adminstration costs!). However, since I pointed out our productivity
vs. input as above (and a few other things), I've had no more hassles from
admin....yet.

It may not work in all cases, and for large units may not be possible (too
many users to keep track of), but it might be worth pointing out how much of
the research output that contributes to your institution's reputation goes
through the microscopy unit.

good luck,
Rosemary

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia


} From: Debby Sherman {dsherman-at-purdue.edu}
} Date: Wed, 02 Mar 2005 15:54:59 -0500
} To: "William P. Sharp" {wsharp-at-asu.edu} , {Microscopy-at-microscopy.com}
} Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Bill,
} We have run into the same problem. After years of having EVERYTHING
} covered we are under increased pressure to cover everything. This has
} gotten worse as University budgets have shrunk. We cover service contracts
} for 3 microscopes (~$60,000) and additional for a full service and
} multi-user facility with TEM, SEM, and LM. We still fall about 15-20% short
} of covering everything (including data and telephone lines, postage, all
} consumables, etc).
}
} The administration's response is to increase rates. I expect increasing
} rates will result in decreased user hours and no gain in revenue...until you
} get to the point where users drop off and revenue decreases. Unfortunately
} non-microscopists do not understand that this is not a speed discipline. It
} takes time to adequately align a scope and thoroughly examine samples. If a
} user is concerned about watching the clock than quality of research is sure
} to suffer. Aren't the facilities here to assist in generating good
} research? Or maybe I have been mistaken about this all these years. With
} hundreds of thousands of dollars invested in instrumentation, you would
} think that universities could find 10-20 thousand each year to help keep
} them accessible to the maximum number of researchers.
}
} Debby
}
} P.S. I am saving responses to Bill's E-mail and intend to give them all to
} my administrators and advisory committee. I think they can do a lot to help
} support my arguments so keep them coming!
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
} On 3/1/05 5:01 PM, "William P. Sharp" {wsharp-at-asu.edu} wrote:
}
} }
} }
} }
-----------------------------------------------------------------------------} }
-
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
------------------------------------------------------------------------------}
}
} -
} }
} } Microscopy Listers -
} }
} } Our perennial lament - does anyone out there know of a University or
} } Institute (non-profit) that operates an EM facility or a Bioimaging
} } facility that takes in enough money through charge back of users to cover
} } most or all of the costs of lab operation, to include service contracts on
} } major instruments? If so, may we know the rates charged? Are there a
} } relatively large number of outside users that are billed at a much higher
} } rate than inside users?
} }
} } We at ASU are in the second year of ramping up the rates charged for zero
} } to an average charge rate over four years and are feeling some pressure to
} } charge enough to pay for all operations of our bioimaging facilities, EM
} } and light as soon as possible. Those of us who are responsible for the
} } facilities are concerned about pricing ourselves out of the market and we
} } need some idea of whether it can be done without a total upheaval of
} } service or the loss of our core facilities.
} }
} } Thanks for any information or advice you may have for us -
} }
} } Bill Sharp
} }
} } William P. Sharp
} } Arizona State University
} } School of Life Sciences, box 4501
} } Tempe, AZ 85287-4501
} } Phone - (480)-965-3210
} } Fax - (480)-965-6899
} }
} }
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 03:27:43 2005



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Thu, 3 Mar 2005 09:34:49 -0000
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just thought I'd say that this kind of problem is not unique to the academic sector, and I have a lot of sympathy for anyone trying to run an independent lab and cover all their costs. When I first started here 10 years ago, we were subject to the same accounting rules as the rest of the business, and had to recover all our costs through user charges - although much of this was in 'wooden dollars'. After a few years, some divisions of the company were spun off into start-ups or moved to other sites, and we started to really struggle to fill in the time sheets every week. We ended up taking problems from engineers inside the company, using our expertise to get some good information, and going back to their manager trying to sell the report. Eventually, after a lot of pushing, we were turned into an overhead for the whole site. The result - a lot more work, more freedom to work on the issues important to key projects and overall a far better contribution to the business. I try to keep a list of the money-burning problems we have helped to solve, just one or two big ones can cover our costs for the year.
The argument that seems to work is "how much money would it cost if we were not here", and although this is intangible, accountants seem much happier with this kind of calculation. Perhaps the same argument can be used in the academic sector - take each paper published which uses microscopy off the list and work out how your institution would then fare in a research assessment exercise, translate that into fewer grants, fewer students, etc. Or the cost of having that work done in a commercial lab. I would think that even being quite cautious with the assumptions you should be able to cover your costs several times over.

Good luck

Richard
________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


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From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 03:57:07 2005



From: Robert H. Olley :      hinmeigeng-at-hotmail.com
Date: Thu, 03 Mar 2005 10:02:59 +0000
Subject: [Microscopy] RE: TEM section stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have played a lot with potassium permanaganate in various conditions, and
most likely when used a a stain one is getting a precipitate of Manganese
Dioxide (Mn02) formed round the most reactice and reducing components of the
cell. I'm not a bio man myself, but I would suggest that the main thing to
play around with is the pH of your staining solution, perhaps using
appropriate buffers. What buffers do you normally use? Citrate, for
example, would be easily oxidised by the permanganate, which would shed Mn02
everywhere.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------


>Has anyone ever used Potassium Permanganate as a section stain?
>Apparently it has been shown to improve the contrast in certain
>types of cell in the artery wall and I wondered what might be going
>on in these cells that their contrast should be so improved with
>this stain. Has anyone a method for the staining?

>Ursula J. Potter
>Centre for Electron Optical Studies (CEOS)
>Building 3 West 2.15
>The University of Bath
>Claverton Down
>Bath BA2 7AY
>UK
>Tel: 01225 385651
>Email: U.J.Potter-at-bath.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 08:22:13 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 03 Mar 2005 09:27:24 -0500
Subject: [Microscopy] RE: Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In response to John's comments about companies using university
facilities because of their low rates...
Our rates are low with respect to commercial firms, BUT that is only
for our "in house" personnel. I am under orders from my dean to bill
ALL outside users (including academics) my usual fees PLUS 70% (the
rate that NIH pays my institution for indirect costs). I believe
that that extra charge brings my rates up to somewhere around what
commercial labs charge. I know of other university facilities that
double their normal rates for all "off campus" users.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 08:38:32 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 03 Mar 2005 09:43:30 -0500
Subject: [Microscopy] Re: RE: Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi John,

These are very good points that you are raising.
However, I don't know how widespread a problem
it is. I have only handled projects for outsiders twice
in the last 6 years, and in both cases it was small
local companies that had been set up by ex-
postdocs from my department. I have to admit that
I have been thinking about advertising our services
to the outside as a way to boost our revenues, but
I gave up on the idea not because I wanted to remain
fair to the competition, but mostly because I want
to remain dedicated to the research community in
my university, even at the risk of running deficits. And
to be fair to our administration, they have allowed us
to run deficits without putting too much pressure on
us, and they have never suggested or encouraged us
to seek business from the outside. Actually, I think
they would be against the idea.
Best

Marc

On Wednesday, March 2, 2005, at 05:49 PM, John Knowles wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Bill et al,
}
} I own and operate a small analytical microscopy lab and like everyone
} else in the dreaded private sector, I have to make a profit every month
} or else I do not get paid. Because of this my focus is naturally on
} serving the client's microscopy needs and not so much on research.
}
} I was always under the impression that Universities were mainly focused
} on research. When part of my tax dollars goes to pay for new equipment
} for University microscopy labs I am ok with it as long as I believe
} that
} it is going to be used for education and/or research projects that the
} University is doing. When I hear that this equipment is being used to
} set up microscopy service labs that are competing against me then I am
} definitely not ok with it. Why should I have to pay for all of my
} equipment, come in on weekends and stay late into the night doing my
} own
} equipment maintenance and service, and pay 100% of my costs when the
} government is using my tax money to help my competition?
}
} There are a number of local Universities in the Boston area that are
} happy to accept government grants for new equipment and then use it to
} take business away from privately owned labs like mine. I have talked
} to many companies that have told me that they go to a University
} Microscopy lab to get there work done instead of a lab like mine
} because
} the Universities rates are so low. If I only had to cover 80% (or
} less)
} of my costs and got grants for my equipment I could drop my rates as
} well. MIT is one of the few Universities that play by the rules. They
} do not take business away from privately own labs like mine. In fact
} they send private companies away and recommend labs like mine to them.
} And for that I am grateful.
}
} I do not think Universities should compete against private labs when
} they are getting government money (just in case you have not figured
} that out by now 8^). If they must then at least raise your rates to
} the
} typical rates in your area. That would make it a fair playing field.
}
} Another thought I had, do your administrators factor in any of the
} students tuition when calculating your profitability? There are many
} students that pick which college they are going to attend based on, at
} least in part, what the facilities are at that location. A microscopy
} department is a big asset to a school that is trying to attract
} students
} interested in the sciences (e.g. biology and materials). When you look
} at a University Microscopy Dept. as a part of the whole education
} system
} then it is silly to think it has to act like a business. The campus
} grounds need to be kept up as well in order for a university to attract
} and keep students. Does your administration ask the grounds crew to be
} profitable as well? Maybe compete with locate landscaping companies
} mowing lawns and trimming bushes. I bet not.
}
} John Knowles
} President
} MicroVision Laboratories, Inc.
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 08:54:15 2005



From: Elaine F. Schumacher :      eschumacher-at-mccrone.com
Date: Thu, 3 Mar 2005 09:00:59 -0600
Subject: [Microscopy] Announcing New Short Courses in Light and Electron Microscopy in 2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Located in Westmont, Illinois, the McCrone College of Microscopy is an
institution for special instruction and specialized education, whose
goal
is to advance the knowledge and understanding of light and electron
microscopy for materials analysis. It is dedicated to quality hands-on
teaching of micro-analytical principles, techniques and applications,
using state-of-the-art light and electron microscopes and accessories.

The following short courses will be offered in 2005:

MCM200: Scanning Electron Microscopy
Dates: April 4-8, 2005

MCM430: Microscopical Identification of White-Powder Unknowns (Part 1)
Dates: April 18-22, 2005

MCM300: Microscopic Particle Handling: Particle Isolation, Manipulation
and Mounting
Dates: May 9-13, 2005

MCM430: Microscopical Identification of White-Powder Unknowns (Part 1)
Dates: June 6-10, 2005

MCM410: Microscopical Identification of Pharmaceutical Materials and
Contaminants
Dates: July 11-15, 2005

MCM140: Microscopical Particle Analysis
Dates: August 8-12, 2005

MCM400: Microscopical Examination of Forensic Trace Evidence
Particles (Part 1)
Dates: September 12-16, 2005

MCM420: Microscopical Identification of Pigments for Art Conservation
and Architectural Restoration Professionals
Dates: September 19-23, 2005

MCM300: Microscopic Particle Handling: Particle Isolation, Manipulation
and Mounting
Dates: October 3-7, 2005

MCM200: Scanning Electron Microscopy
Dates: October 17-21, 2005

MCM100: Polarized Light and Chemical Microscopy
Dates: November 14-18, 2005

These courses are presented by instructors with distinguished records,
experience and expertise in polarized light microscopy and scanning
electron microscopy. For instructor biographies, course details and
other information, go to www.mccrone.com. Inquiries can be directed to
courses-at-mcccrone.com.

Thank you.

Charles A. Zona
Vice President
Director of Education
McCrone College of Microscopy
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559
Telephone 630-887-7100
Email: czona-at-mccrone.com
Website: www.mccrone.com




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 10:14:33 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 3 Mar 2005 10:21:37 -0600
Subject: [Microscopy] Cost recovery at imaging facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have also been dealing with this issue for some time, and although we
are under pressure to increase revenues and it is made clear that the
ideal is to recover all costs, the pressure is not unduly severe. Part
of the reason, I think, is that we have been aggressive in recent years
in trying to increase our revenue flow and user base (as I'm sure many
labs have been), and we have quadrupled our usage since 2000.

Currently we recover all but about 30% of direct expenses, as close as I
can tell. This includes service contracts, consumables, and partial
salaries, although not building overhead and equipment replacement,
which of course would take our percentage way down if we included them
as direct costs.

But it is important to note that we have an educational component to our
facility's mission, as well as a research one. We run two courses out
of our lab and assist with lab facilities for an outside class. We
also provide tours, lectures, and workshops, usually at no charge, for
courses, local schools, and other universities. We run fee-based
workshops and short courses on occasion on a break-even basis (if we're
lucky!), and we quite often are used as part of the itinerary for
recruitment of prospective faculty and sometimes graduate students. All
of this represents a lot of time and effort taken away from just serving
our clients' research needs and from new protocol development. How do
we measure the financial value of this to our university?

I think it's safe to say that this issue will never go away, especially
in these precarious budgeting times, but as the replies to this question
are showing, there are many arguments that can be put forward that
research imaging facilities generate great benefits for their
institutions that are not always easy to quantify in dollar terms. That
doesn't mean they don't exist and that doesn't mean we should apologize
for somehow being inadequate in the eyes of the accountants. So far,
I've never been charged a fee for using our university library and I
expect their cost-recovery is....ahem....somewhat lower than ours.

My two coppers worth.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 10:58:24 2005



From: Carla M Conway :      cmconway-at-usgs.gov
Date: Thu, 3 Mar 2005 09:04:58 -0800
Subject: [Microscopy] permeabilization needed in IHC of viral protein?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






Hello,

I am interested in using IHC to detect nucleocapsid (N) protein within
rhabdovirus. I have had success in detecting the glycoprotein on the
envelope surface, however the N protein is located within the virion.
Sections (FFPE) will be enzymatically digested with 0.05% protease XIV in
TBS according to our standard protocol. Would a permeabilization agent
increase access to the interior proteins? Any suggestions/comments will be
greatly appreciated.


Thank you,

Carla Conway
Western Fisheries Research Center
6505 NE 65th St
Seattle, WA 98115
ph: 206-526-6282 ext. 242
fax: 206-526-6654





From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 10:59:08 2005



From: sghoshro-at-NMSU.Edu
Date: Thu, 3 Mar 2005 10:05:24 -0700 (MST)
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The timing of Bill's e-mail to the list serve couldn't be better. Last
week my university (a relatively small institution) administrators brought
a proposal on the table that the EM Lab is too expensive and costing too
much to the university. Being in nowhereland, we hardly get outside users
and we have a fairly small user base. However, it is the one and only EM
facility on campus that serves both materials and biological users. The
administrators did agree that this facility helps them to sell the
university for prospective students and faculty. However, as many people
mentioned that shortsightedness in their part is playing a big role here.

So far we have generated a huge support against this move and I hope it
will help to maintain the lab. We generate a small amount of revenues and
we have one TEM and one SEM(with EDS) and an upright fluorescence scope.
The lab has two staff (one full time, one part time) members and their
salaries come from the college Arts & Sciences and Biology department. The
revenues are mostly used for buying all lab supplies, computer upgrades,
small instrument repairs and occasionally to pay for a student assistant.

We offer a grad level EM course every fall thrpough Biology and is quite
popular among grad students. The course costs $1000.00 every year for
Biology and eight students (max) can enroll due to limited resources.
Department chair mentioned to me that this course is too expensive since
only eight students can take it.

Therefore, my question is to the people who are running small university
facilities if they are facing similar problems from their administrators.

Thanks a lot,

Soumitra


*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/

On Tue, 1 Mar 2005, William P. Sharp wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Microscopy Listers -
}
} Our perennial lament - does anyone out there know of a University or
} Institute (non-profit) that operates an EM facility or a Bioimaging facility
} that takes in enough money through charge back of users to cover most or all
} of the costs of lab operation, to include service contracts on major
} instruments? If so, may we know the rates charged? Are there a relatively
} large number of outside users that are billed at a much higher rate than
} inside users?
}
} We at ASU are in the second year of ramping up the rates charged for zero to
} an average charge rate over four years and are feeling some pressure to
} charge enough to pay for all operations of our bioimaging facilities, EM and
} light as soon as possible. Those of us who are responsible for the facilities
} are concerned about pricing ourselves out of the market and we need some idea
} of whether it can be done without a total upheaval of service or the loss of
} our core facilities.
}
} Thanks for any information or advice you may have for us -
}
} Bill Sharp
}
} William P. Sharp
} Arizona State University
} School of Life Sciences, box 4501
} Tempe, AZ 85287-4501
} Phone - (480)-965-3210
} Fax - (480)-965-6899
}
}


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 11:00:19 2005



From: Hong Yi :      hyi-at-emory.edu
Date: Thu, 3 Mar 2005 12:07:24 -0500
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Everyone:

My facility has been doing relatively well in being self-sufficient
for the last 5 years. So I was surprised when a friend of mine in
Florida told me that her facility is 100% self-sustained. Even more
stunning to me was that her rate in general was lower than mine. She
had to recover everything including salaries, supplies, service
contracts etc. She told me her trick was to charge for everything.
She even charged for time she spent to email users. She also told me
that she does not have any complain from users about her rate.

I joked to her that she is someone I do not want my dean to know
about. ;-).

HOng
Emory EM



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 11:02:02 2005



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Thu, 03 Mar 2005 11:20:03 -0600
Subject: [Microscopy] MMMS Meeting March 24th, 2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} From a fair competition point of view, I am glad to hear that most if
not all university facilities increase their fees to outside users. And
70% to 100% increases in your standard rates sounds like a lot. But
when the standard in house rates are on the order of $40 to $50 dollars
per hour for TEM or SEM/EDS (those are university in house rates I have
heard about in the Boston area) then the outside user rates still end up
being about half of what the norm for private microscopy labs is in my
area. And that could tempt even the most loyal of our clients.

But as someone else noted, all is not lost for those of us with the
private labs since we can compete in other areas (service, turn around
times, quality, advertising, location, etc.). And frankly right now
there is more work out there, if you know where to look, then the
university labs can handle, much more. And I do not think doing outside
work is what the staff at university labs want to do all day anyway. So
the overall impact to private sector labs, like mine, is likely much
less we often think.

Sorry if I sounded hostile in my last post. It is just that you guys
get so many nice toys to play with, and for free. But then again I do
not have to deal with any administration types or write grant proposals.
Guess the grass is always greener...

John Knowles
MicroVision Laboratories, Inc.



-----Original Message-----
} From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
Sent: Thursday, March 03, 2005 9:27 AM
To: John Knowles; 'Debby Sherman'; 'William P. Sharp';
Microscopy-at-microscopy.com

Local MSA, MAS affiliate meeting announcement

Imaging Biomolecular Interactions
Midwest Microscopy and Microanalysis Society
Co-sponsor: Biological Imaging Facility, Northwestern University

Thursday, March 24, 2005

Northwestern University
Pancoe Life Sciences Building
Pancoe/ENH Auditorium
Evanston, IL 60208

Meeting Schedule
Morning Session
9:00 Registration
9:45 Welcome and introduction
10:00 Victoria Froelich, University of Texas Health Sciences Center San
Antonio: FRET, dectection of molecular interactions.
11:00 Kanya Rajangam, Northwestern University: Cells in self-assembling gels.
11:30 Carina Holmberg and Heather Brignull, Northwestern University:
Imaging-based characterization of aggregates in neurodegenerative disease
models.
12:00-12:45 Lunch
12:45 Business Meeting

Afternoon session
1:00 H. Peter Lu, Paci. c Northwest National Laboratory: Single-molecule
biophysics.
2:00 Debbie Klos, Northwestern University: Recognition of membrane protein
cargo by the Rsp5 ubiquitin ligase.
2:30 Victoria Wu, Northwestern University: The expansive tube, the role of
the septate junction in Drosophila tracheal development.
3:00 Reception and Student Poster Competition Exhibit and Judging.

RSVP Required
Please send RSVP via email, phone, or fax to:
Arvid Casler, MMMS Program Coordinator
c/o MAI
P. O. Box 394
Mundelein, IL 60060
phone/FAX: (847) 566-7716
email: arvid_casler-at-fmo.com


Admission: Free to MMMS Members
MMMS Membership: $10.00
MMMS membership available at registration

Driving directions to Northwestern University Evanston Campus available on
the Web.
From the north or northwest, via Interstate 88, Interstate 90 or
Interstate 190
(Note: these are the directions to follow if traveling from O'Hare
International Airport)
http://www.northwestern.edu/campus/directions/evanston-north-northwest.html
From the west, via Interstate 94
http://www.northwestern.edu/campus/directions/evanston-west.html
From the west or southwest, via Interstate 55 or Interstate 80
http://www.northwestern.edu/campus/directions/evanston-west-southwest.html
From the south or southeast, via Interstate 94, Interstate 90, Interstate
80 or Interstate 57
http://www.northwestern.edu/campus/directions/evanston-south-southeast.html

PARKING
Parking permits are $4 and are nonrefundable.
Parking can be challenging on the NU Evanston Campus especially after 9AM.
Spaces in selected lots are available on a first-come-first-served basis.



Alan W Nicholls, PhD
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 11:24:13 2005



From: Aleksandr Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Thu, 03 Mar 2005 17:34:01 +0000
Subject: [Microscopy] Re: TEM EM Atlas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you everybody for the information about EM Atlas!

It seems that the most popular EM atlases are the ones by Fawcett and by
Johannes A G Rhodin.

I will try to find them.

--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 11:28:23 2005



From: Aleksandr Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Thu, 03 Mar 2005 17:38:12 +0000
Subject: [Microscopy] TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear collegues!

Just recently I found the structure, which I cannot identify. It has
regular hexagonal pattern and localized in the cytosol of the cells in
embryonic tendons. It is not membrabous, some kind of cytoskeletal?

Could you, please, take a look and hint what it could be?
The address for pictures is (structure is identified by arrows):
http://sysoj.spymac.net/image.jpg
http://sysoj.spymac.net/image2.jpg

Regards,
--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 11:47:08 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Thu, 3 Mar 2005 09:53:16 -0800
Subject: [Microscopy] RE: Re: RE: Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John;
You are right to be dismayed. Companies are so cost obsessed
that they will do anything to save a buck, and that includes getting as
much for nearly free as they can. My own company, a $30 billion Fortune
500 company, pushed for using university labs for years, just to avoid
the cost of purchasing, housing, staffing, and maintaining a TEM,
something they could easily afford. Some people just have no shame.

John Mardinly

} Bill et al,
}
} I own and operate a small analytical microscopy lab and like everyone
} else in the dreaded private sector, I have to make a profit every
month
} or else I do not get paid. Because of this my focus is naturally on
} serving the client's microscopy needs and not so much on research.
}
} I was always under the impression that Universities were mainly
focused
} on research. When part of my tax dollars goes to pay for new
equipment
} for University microscopy labs I am ok with it as long as I believe
} that
} it is going to be used for education and/or research projects that the
} University is doing. When I hear that this equipment is being used to
} set up microscopy service labs that are competing against me then I am
} definitely not ok with it. Why should I have to pay for all of my
} equipment, come in on weekends and stay late into the night doing my
} own
} equipment maintenance and service, and pay 100% of my costs when the
} government is using my tax money to help my competition?
}
} There are a number of local Universities in the Boston area that are
} happy to accept government grants for new equipment and then use it to
} take business away from privately owned labs like mine. I have talked
} to many companies that have told me that they go to a University
} Microscopy lab to get there work done instead of a lab like mine
} because
} the Universities rates are so low. If I only had to cover 80% (or
} less)
} of my costs and got grants for my equipment I could drop my rates as
} well. MIT is one of the few Universities that play by the rules.
They
} do not take business away from privately own labs like mine. In fact
} they send private companies away and recommend labs like mine to them.
} And for that I am grateful.
}
} I do not think Universities should compete against private labs when
} they are getting government money (just in case you have not figured
} that out by now 8^). If they must then at least raise your rates to
} the
} typical rates in your area. That would make it a fair playing field.
}
} Another thought I had, do your administrators factor in any of the
} students tuition when calculating your profitability? There are many
} students that pick which college they are going to attend based on, at
} least in part, what the facilities are at that location. A microscopy
} department is a big asset to a school that is trying to attract
} students
} interested in the sciences (e.g. biology and materials). When you
look
} at a University Microscopy Dept. as a part of the whole education
} system
} then it is silly to think it has to act like a business. The campus
} grounds need to be kept up as well in order for a university to
attract
} and keep students. Does your administration ask the grounds crew to
be
} profitable as well? Maybe compete with locate landscaping companies
} mowing lawns and trimming bushes. I bet not.
}
} John Knowles
} President
} MicroVision Laboratories, Inc.
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446





From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 13:02:38 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 03 Mar 2005 14:09:02 -0500
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} She sounds like a lawyer....



} Dear Everyone:
}
} My facility has been doing relatively well in being
} self-sufficient for the last 5 years. So I was surprised when a
} friend of mine in Florida told me that her facility is 100%
} self-sustained. Even more stunning to me was that her rate in
} general was lower than mine. She had to recover everything
} including salaries, supplies, service contracts etc. She told me
} her trick was to charge for everything. She even charged for time
} she spent to email users. She also told me that she does not have
} any complain from users about her rate.
}
} I joked to her that she is someone I do not want my dean to
} know about. ;-).
}
} HOng
} Emory EM


--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 13:06:26 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 03 Mar 2005 14:12:52 -0500
Subject: [Microscopy] Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Aleksandr...
your dissection was a little mis-directed. Those are thin and thick
muscle filaments in cross section. Your sample must have come from
the point of muscle-tendon junction.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 14:07:57 2005



From: Jay Campbell :      microtomy-at-gmail.com
Date: Thu, 3 Mar 2005 14:14:11 -0600
Subject: [Microscopy] LASIK correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello microscopists,
A quick check of the archives indicates that it has been almost 4
years since there was much discussion about this topic on list. Given
the pace of technology and the popularity of the proceedure, it seems
fitting to visit the topic again. I have recently been evaluated and
declared an excellent candidate for LASIK correction. I was hoping
that others who've opted for this surgery could share their
experience, positive or negative, to help me make my decision.

Thanks much and I will be happy to post a compilation of the responses,
Jay


Jay Campbell
Research Specialist
University of Wisconsin
Laboratory of Molecular Biology
R.M. Bock Labs
1525 Linden Drive
Madison, WI 53706
jmcampbe-at-wisc.edu
608 263 8481


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 14:48:14 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 03 Mar 2005 15:54:46 -0500
Subject: [Microscopy] Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'll take a stab at it and say it looks like virus in a crystalline array
although size appears a bit small.

Is there any signs of fibers in longitudinal orientation? If it is made up
of cytoskeletal elements, i.e. fibers of different diameters, than I would
expect to see some in longitudinal as well as x-sectional view.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 3/3/05 12:38 PM, "Aleksandr Mironov" {Aleksandr.Mironov-at-manchester.ac.uk}
wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Dear collegues!
}
} Just recently I found the structure, which I cannot identify. It has
} regular hexagonal pattern and localized in the cytosol of the cells in
} embryonic tendons. It is not membrabous, some kind of cytoskeletal?
}
} Could you, please, take a look and hint what it could be?
} The address for pictures is (structure is identified by arrows):
} http://sysoj.spymac.net/image.jpg
} http://sysoj.spymac.net/image2.jpg
}
} Regards,




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 15:06:28 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Thu, 03 Mar 2005 15:19:14 -0600
Subject: [Microscopy] Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

aleksandr

it is not the best way to look at these things, all i have is a picture,
no size bars, etc. also, are the cells tissue culture, are they an
explant, or are they harvested tissue which you have embedded?

having said those things, looking at the paracrystaline nature makes me
think virus. have you considered lysing the cells and looking at the
gemisch by negative stain? that would confirm or reject the possibility
of virus.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-954
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 15:58:28 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 03 Mar 2005 14:08:06 -0800
Subject: [Microscopy] Re: RE: Re: Cost Recovery in Imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John
Very good point. I think, the University's facilities (including EM)
should serve scientific community and stay away from the business (I meant
business of "generating" money). In my prospective, "business" orientation
of the University Labs seriously affected the quality of research: they
tends to use "standard" simple protocols and users don't want to pay for
extra hour to develop better protocol for their particular case. I think,
most of us here at ListServer do understand this perfectly. The problem is
that Universities are struggled from money deficit and we are a good
targets to blame: we are using too much money... not "profitable"...
etc; how you could measure "profit" in science?). To be truthful, I have
to admit that I don't feel such pressure at UCLA and I met full
understanding that I am serving Science. Therefore my facility is heavily
subsidized. I also have rates for outsiders 6x higher than for insiders -
mostly to keep them away, so I have more time for students etc. Rates for
insiders are diversified, so many users use instruments for free. I clearly
understand that my "good life" may ended any moment and I'll join "the
club" switching from doing science to searching for the money... Have a
great day, Sergey

At 05:49 PM 3/2/2005 -0500, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 16:04:47 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Thu, 03 Mar 2005 16:17:33 -0600
Subject: [Microscopy] Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

aleksandr

another possibility is that you have actin/myosin filaments in muscle
fibres in cross section. it has been a long time since i've looked at
smooth muscle but i don't think it would match for that. it would not
fit for the standard cross section of a skeletal muscle cell, i would
expect more ordered fibres. but i do not know what the fibres would
look like in a myocyte at the edge of an embryonic tendon. again,
problem is lack of size markers makes it difficult

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-954
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 16:39:53 2005



From: stuart McKernan :      stuartm-at-umn.edu
Date: Thu, 3 Mar 2005 16:46:52 -0600
Subject: [Microscopy] LAS meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Minnesota Microscopy Society will be co-hosting the 3rd Annual
Minnesota Joint Technical Symposium
(MinnTS 2005) on Thursday, March 17, 2005. Any interested
microscopists will be welcome to attend. This year the topic is
Biomaterials and Biotechnology, and we will have two speakers:

Vincent Chow , Optobionics, www.optobionics.com
"Artificial Retina"
and
Arthur J. Coury, Genzyme Corporation,
"Therapeutic Strategies, from Replacement Parts to Regenerative
Medicine: Challenges and Opportunities"

The Schedule for the afternoon is as follows
Schedule of Events
4:30 - 5:30 Registration, tours, and social
5:30 - 6:40 Dinner
6:45 - 7:00 Welcome message
7:00 - 7:50 Vincent Chow
7:50 - 8:00 Break
8:05 - 8:55 Arthur Coury

The cost for the event will be $25 including dinner. Reservations
should be addressed to: Bede Willenbring, (651) 236-5430 or
Bede.Willenbring-at-HBFuller.com by March 11, 2005.

Please include your name and company, email address and phone number
with your registration, as well as whether you are a member of any of
the sponsoring societies:
American Chemical Society, Minnesota chapter (ACS)
American Institute of Chemical Engineers, Upper Midwest Section
(AIChE)
American Society for Materials, Minnesota chapter (ASM)
American Vacuum Society, Minnesota Chapter (AVS)
Minnesota Microscopy Society (MMS)
Society for Applied Spectroscopy, Minnesota chapter (SAS)
SEMI-MN
Society for Information Display, Midwest chapter (SID)

More details can be found on our website http://www.MNmicroscopy.org


Prof. Stuart McKernan
IT Characterization Facility, University of Minnesota            
E-mail :  stuartm-at-umn.edu
12 Shepherd Labs,                                                    
                 Office:         (612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455                    
Lab:            (612) 626-7594





From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 17:38:44 2005



From: Schmitz, Bob :      rschmitz-at-uwsp.edu
Date: Thu, 3 Mar 2005 17:45:41 -0600
Subject: [Microscopy] Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Since you indicate that this is embryonic tendon, is there any chance that these cells are not fibroblast like but muscle like? Those hexagonal arrays look similar to the cross-sections of skeletal muscle myofibrils where the thick and thin filaments overlap.

Bob
Robert J. Schmitz
Department of Biology
University of Wisconsin Stevens Point
Stevens Point, WI 54481
715-346-2420
Email: rschmitz-at-uwsp.edu
http://biology.uwsp.edu/faculty/RSchmitz/Default.htm



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 18:11:27 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 03 Mar 2005 19:17:59 -0500
Subject: [Microscopy] Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Surely these must be myofibrils, in cross section.
A cell differentiating into a myocyte maybe,
which could be expected in embryonic tissue.
Or a smooth muscle cell?
I would opt for the former personally.

Best wishes

Marc

On Thursday, March 3, 2005, at 12:38 PM, Aleksandr Mironov wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Dear collegues!
}
} Just recently I found the structure, which I cannot identify. It has
} regular hexagonal pattern and localized in the cytosol of the cells in
} embryonic tendons. It is not membrabous, some kind of cytoskeletal?
}
} Could you, please, take a look and hint what it could be?
} The address for pictures is (structure is identified by arrows):
} http://sysoj.spymac.net/image.jpg
} http://sysoj.spymac.net/image2.jpg
}
} Regards,
} --
} Aleksandr Mironov
} Experimental Officer
} G450A, Stopford Building
} EM Unit, Faculty of Life Sciences
} University of Manchester
} Oxford Road
} Manchester
} M13 9PT
} UK
}
} Tel. +44-(0)161-275-7395
} Fax. +44-(0)161-275-5171
} E-mail: Aleksandr.Mironov-at-manchester.ac.uk
} MSN: amironov-at-hotmail.co.uk
} Web: http://www.empgu.man.ac.uk
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 3 21:50:20 2005



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 03 Mar 2005 19:56:54 -0800
Subject: [Microscopy] RE: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HAMLET Do you see yonder cloud that's almost in shape of a camel?
POLONIUS By th'mass, and 'tis: like camel, indeed.
HAMLET Methinks it is like a weasel.
POLONIUS It is backed like a weasel.
HAMLET Or like a whale.
POLONIUS Very like a whale.
William Shakespeare, Hamlet, c. 1600 [Act 3, scene 2]

I must chuckle. Have you looked at other cells and found these structures
in different planes of view, hence allowing better differentiation?

The n of 1 makes for a tough puzzle.

:-) Ken

_______________________________________
Kenneth L. Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N Willamette Blvd.
Portland, OR 97203 USA

Tel.: 503.493.8861






From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 01:39:26 2005



From: Gareth Morgan :      Gareth.Morgan-at-labmed.ki.se
Date: Fri, 04 Mar 2005 08:46:46 +0100
Subject: [Microscopy] Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Look like rhabdomyocyte/blast actin/myosin contractile fibres to me - would
be seen in that typical array. I don't think virus (but then I am not a
virologist :-)).

A scale would be nice but I can see the problem really - there are enough
identifiable structures to be able to 'size' the array. Might be different
if I was going to court about it!

Good luck with your hunt for Bloom and for Rhodin - I still have my old
copies - wonderful books.


Gareth


With best wishes,

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Histo/cytopathology, Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 02:06:51 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 4 Mar 2005 00:12:40 -0800
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Soumitra;
Remind your administrators about the super conducting
supercollider. It was cancelled, and now virtually all of the high
energy physics research (and all the physicists) is done in Europe!

John Mardinly
Intel

-----Original Message-----
} From: sghoshro-at-NMSU.Edu [mailto:sghoshro-at-NMSU.Edu]
Sent: Thursday, March 03, 2005 9:05 AM
To: William P. Sharp
Cc: Microscopy-at-microscopy.com

The timing of Bill's e-mail to the list serve couldn't be better. Last
week my university (a relatively small institution) administrators
brought
a proposal on the table that the EM Lab is too expensive and costing too

much to the university. Being in nowhereland, we hardly get outside
users
and we have a fairly small user base. However, it is the one and only EM

facility on campus that serves both materials and biological users. The
administrators did agree that this facility helps them to sell the
university for prospective students and faculty. However, as many people

mentioned that shortsightedness in their part is playing a big role
here.

So far we have generated a huge support against this move and I hope it
will help to maintain the lab. We generate a small amount of revenues
and
we have one TEM and one SEM(with EDS) and an upright fluorescence scope.

The lab has two staff (one full time, one part time) members and their
salaries come from the college Arts & Sciences and Biology department.
The
revenues are mostly used for buying all lab supplies, computer upgrades,

small instrument repairs and occasionally to pay for a student
assistant.

We offer a grad level EM course every fall thrpough Biology and is quite

popular among grad students. The course costs $1000.00 every year for
Biology and eight students (max) can enroll due to limited resources.
Department chair mentioned to me that this course is too expensive since

only eight students can take it.

Therefore, my question is to the people who are running small university

facilities if they are facing similar problems from their
administrators.

Thanks a lot,

Soumitra


*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/

On Tue, 1 Mar 2005, William P. Sharp wrote:

}
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}
} Microscopy Listers -
}
} Our perennial lament - does anyone out there know of a University or
} Institute (non-profit) that operates an EM facility or a Bioimaging
facility
} that takes in enough money through charge back of users to cover most
or all
} of the costs of lab operation, to include service contracts on major
} instruments? If so, may we know the rates charged? Are there a
relatively
} large number of outside users that are billed at a much higher rate
than
} inside users?
}
} We at ASU are in the second year of ramping up the rates charged for
zero to
} an average charge rate over four years and are feeling some pressure
to
} charge enough to pay for all operations of our bioimaging facilities,
EM and
} light as soon as possible. Those of us who are responsible for the
facilities
} are concerned about pricing ourselves out of the market and we need
some idea
} of whether it can be done without a total upheaval of service or the
loss of
} our core facilities.
}
} Thanks for any information or advice you may have for us -
}
} Bill Sharp
}
} William P. Sharp
} Arizona State University
} School of Life Sciences, box 4501
} Tempe, AZ 85287-4501
} Phone - (480)-965-3210
} Fax - (480)-965-6899
}
}




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 03:16:10 2005



From: Gareth Morgan :      Gareth.Morgan-at-labmed.ki.se
Date: Fri, 04 Mar 2005 10:23:45 +0100
Subject: [Microscopy] Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

'can' in my previous mail should of course be 'can't' - corrected version below


Hi

Look like rhabdomyocyte/blast contractile fibres to me - would be seen in
that typical array. I don't think virus (but then I am not a virologist :-)).

A scale would be nice but I can't see the problem really - there are enough
identifiable structures to be able to 'size' the array. Might be different
if I was going to court about it.

Good luck with your hunt for Bloom and for Rhodin - I still have my old
copies - wonderful books.

Gareth

With best wishes,

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Histo/cytopathology, Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 04:13:48 2005



From: Aleksandr Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Fri, 04 Mar 2005 10:23:15 +0000
Subject: [Microscopy] Re: Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you a lot for your advices!

Sorry, that I do not have more decent image to show and that I forgot to
put a scale bar.

Two suggestions were made:
1) It is a virus
2) It is a forming myofibrilles in embryonic myocyte

We have checked serial sections from the sample (tail from mouse embryo)
and it seems that this structure goes through several of them. The cell
containing the strucutre is located just nearby the tendon. So, the most
probable variant is that these are myofibrilles.

Thank you very much again!
Certainly I need to find some good EM Atlas to educate myself better :-)

--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 04:37:37 2005



From: shashi singh :      shashis_99-at-yahoo.com
Date: Fri, 4 Mar 2005 02:44:16 -0800 (PST)
Subject: [Microscopy] internal structure by TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I feel they may be ribosomes. This would be a period
of high protein synthesis and ribosomes can form such
arrays in many tissues.
ANY prizes for correct guessing!

Shashi Singh
CCMB
Hyderabad
INDIA

=====
Shashi Singh
Scientist
Centre for Cellular and Molecular Biology
Hyderabad-500 007
INDIA
PH-91-40-7192575,7192761,7192615
FAX-91-40-7160591, 7160311




__________________________________
Celebrate Yahoo!'s 10th Birthday!
Yahoo! Netrospective: 100 Moments of the Web
http://birthday.yahoo.com/netrospective/


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 10:06:15 2005



From: Franklin Bailey :      jfb-at-uidaho.edu
Date: Fri, 04 Mar 2005 08:20:27 -0800
Subject: [Microscopy] LASIK correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jay,
I had the correction operation in 2001 and would do it again in a heartbeat.
Now the only correction I need is the typical old folks reading glasses.
Since I was wearing bifocals before the procedure, reading glasses were a
given. It's great to be able to look outside and see the horizon in clear
and sharp focus.

Franklin Bailey
Electron Microscopy Center
University of Idaho
Moscow, ID 83844-2204

-----Original Message-----
} From: Jay Campbell [mailto:microtomy-at-gmail.com]
Sent: Thursday, March 03, 2005 12:14 PM
To: Microscopy-at-microscopy.com

Hello microscopists,
A quick check of the archives indicates that it has been almost 4
years since there was much discussion about this topic on list. Given
the pace of technology and the popularity of the proceedure, it seems
fitting to visit the topic again. I have recently been evaluated and
declared an excellent candidate for LASIK correction. I was hoping
that others who've opted for this surgery could share their
experience, positive or negative, to help me make my decision.

Thanks much and I will be happy to post a compilation of the responses,
Jay


Jay Campbell
Research Specialist
University of Wisconsin
Laboratory of Molecular Biology
R.M. Bock Labs
1525 Linden Drive
Madison, WI 53706
jmcampbe-at-wisc.edu
608 263 8481



From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 10:15:42 2005



From: Franklin Bailey :      jfb-at-uidaho.edu
Date: Fri, 04 Mar 2005 08:30:03 -0800
Subject: [Microscopy] TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Many years ago when I was at the University of Texas Medical Branch in
Galveston working for Dr. Ward Kischer, we did extensive research on scar
tissue and the granulation tissue of burned skin. Based on the experience
of looking at thousands of sections of connective tissue, I believe the
cells to actually be fibroblasts and the particles to be cross sections of
collagen fibrils, which can range anywhere from 10 to over 200 nm in
diameter, and are produced by the fibroblasts.

Franklin Bailey
Electron Microscopy Center
University of Idaho
Moscow, ID 83844-2204

-----Original Message-----
} From: Aleksandr Mironov [mailto:Aleksandr.Mironov-at-manchester.ac.uk]
Sent: Thursday, March 03, 2005 9:38 AM
To: Microscopy-at-microscopy.com

Dear collegues!

Just recently I found the structure, which I cannot identify. It has
regular hexagonal pattern and localized in the cytosol of the cells in
embryonic tendons. It is not membrabous, some kind of cytoskeletal?

Could you, please, take a look and hint what it could be?
The address for pictures is (structure is identified by arrows):
http://sysoj.spymac.net/image.jpg
http://sysoj.spymac.net/image2.jpg

Regards,
--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk





From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 10:19:01 2005



From: Evren Cubukcu :      ecubukcu-at-hacettepe.edu.tr
Date: Fri, 04 Mar 2005 18:25:54 +0200
Subject: [Microscopy] SEM - LEO or JEOL?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi there;
We have been trying to decide which SEM is worth buying.
JEOL JSM-6380 LV (with PGT Avalon AV 8000 - Sahara EDS system)
or
CARL ZEISS (LEO) EVO 40 XVP (with Quantax, QX2 EDS system)
The instrument will be used for geological purposes and most of the
specimens will be rocks.
We think that EVO 40 XVP is better with motorized 5 axis, no water
cooling, turbomolecular pump... And Quantax EDS (Rontec x-flash
dedector) is amazing being standartless and LN free and over 400,000
cps.
I really need to hear comments on both instruments from users.

Thank You

Evren Cubukcu
Hacettepe University
Dept. Geol. Eng.
SEMProbe Lab.
Ankara
Turkey




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 11:25:07 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Fri, 4 Mar 2005 14:02:05 -0330
Subject: [Microscopy] automatic dessicating cabinets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is anyone familiar with the automatic dessicating cabinets available ...
e.g., "SECADOR" cabinets which are available from Electron Microscopy
Sciences. How do they work? More importantly, how well do they work?

tia & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
{www.micro-investigations.com}



From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 11:50:15 2005



From: White, Woody N. :      NWWhite-at-bwxt.com
Date: Fri, 4 Mar 2005 12:55:39 -0500
Subject: [Microscopy] SEM - LEO or JEOL?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't have experience with either system so have only one comment.
For geological work, I would expect light element capability would be
important to you. If so, be sure to carefully evaluate the low energy
sensitivity and resolution of LN2 free detectors.

Woody



-----Original Message-----
} From: ecubukcu-at-hacettepe.edu.tr [mailto:ecubukcu-at-hacettepe.edu.tr]
Sent: Friday, March 04, 2005 11:26 AM
To: Microscopy-at-microscopy.com

Hi there;
We have been trying to decide which SEM is worth buying.
JEOL JSM-6380 LV (with PGT Avalon AV 8000 - Sahara EDS system)
or
CARL ZEISS (LEO) EVO 40 XVP (with Quantax, QX2 EDS system)
The instrument will be used for geological purposes and most of the
specimens will be rocks.
We think that EVO 40 XVP is better with motorized 5 axis, no water
cooling, turbomolecular pump... And Quantax EDS (Rontec x-flash
dedector) is amazing being standartless and LN free and over 400,000
cps.
I really need to hear comments on both instruments from users.

Thank You

Evren Cubukcu
Hacettepe University
Dept. Geol. Eng.
SEMProbe Lab.
Ankara
Turkey






From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 12:46:09 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Fri, 4 Mar 2005 10:52:40 -0800 (PST)
Subject: [Microscopy] Re: LASIK correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i personaly prefer contacts over a laser in the eye.
better than a sharp stick i guess.
--- Jay Campbell {microtomy-at-gmail.com} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Hello microscopists,
} A quick check of the archives indicates that it has
} been almost 4
} years since there was much discussion about this
} topic on list. Given
} the pace of technology and the popularity of the
} proceedure, it seems
} fitting to visit the topic again. I have recently
} been evaluated and
} declared an excellent candidate for LASIK
} correction. I was hoping
} that others who've opted for this surgery could
} share their
} experience, positive or negative, to help me make my
} decision.
}
} Thanks much and I will be happy to post a
} compilation of the responses,
} Jay
}
}
} Jay Campbell
} Research Specialist
} University of Wisconsin
} Laboratory of Molecular Biology
} R.M. Bock Labs
} 1525 Linden Drive
} Madison, WI 53706
} jmcampbe-at-wisc.edu
} 608 263 8481
}
}




__________________________________
Celebrate Yahoo!'s 10th Birthday!
Yahoo! Netrospective: 100 Moments of the Web
http://birthday.yahoo.com/netrospective/


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 13:42:08 2005



From: Daniel Geiger :      geiger-at-vetigastropoda.com
Date: Fri, 4 Mar 2005 11:48:32 -0800
Subject: [Microscopy] Re: SEM - LEO or JEOL?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Evren,

we just installed in January an Zeiss EVO 40 XVP and went through he
same evaluation process. We use it mainly for biological samples
many of them uncoated, but no EDS or similar application.

The main advantages I see for the EVO are:
- No need for water cooling
- True continuous zoom control of magnification (not stepped: has
driven me crazy on other instruments).
- Most other adjustments are also truly continuous (e.g., EHT, spot
size/probe current, detector bias, VP), not stepped.
- large image files (3200 x 2300 pixels, 6.7 MP)
- Signal mixing (e.g., continuous mixing of SE/VPSE signal with
selected quadrants of QBSD detector): that is REALLY helpful
particularly with uncoated specimen.

Low mag is a bit limited for VP mode/fixed aperture in place with
about 10-15 mm specimen being the largest to be imaged, depending on
WD and ETH. In HiVac, though you can get up to about 25 mm.

I have used a Hitachi 3000N with ESED detector, and the Zeiss VPSE
detector is by far better. If I recall correctly, JEOL does not
produces a variable pressure/environmental SE detector. The number of
options for noise reduction and dealing with charging are very nice.

Re motorized stage, the five axes are great, though the tilt lever is
set-up backwards: the + tilt is pushing to the right, the computer
screen icon tilts to the right, but the stage actually tilts to the
left. So when you visualize the stage tilt using the IR scope (which
is at the front of the chamber, so you get a non-inverted image of
the chamber) you get a bit cross-eyed. I trust Zeiss can fix that on
your instrument.

Get the extended desktop option, so you can use one mouse to go to
two screens. There is also an option for having two live images (say
SE and BSD). The last cool thing is the "$7000 keyboard". It is a
custom keyboard with the main controls as knobs (mag, focus,
stigmation, gun tilt, gun shift, image rotate, scan speed, reduce
raster, brightness contrast) The actual keyboard is like that of a
laptop computer, i.e. without numerical keypad. It is a great time
saver and you may avoid repetitive stress problems when using a mouse
for a long time. I've done some student training, and I think the
keyboard is a bit more intuitive than screen controls so it speeds
up the learning process.

Last but not least, the simplicity of the instrument is awe
inspiring. There are just 5 computer boards in the machine, and very
few cables running around the back. I think my desktop computer with
printer and network connection has more cables than the SEM!


Obviously, I am a happy user. Just for the record, I have no
interests in the Carl Zeiss company other then being a heavy user of
their products (photo, LM, SEM).

Best wishes Daniel


At 6:25 PM +0200 3/4/05, Evren Cubukcu wrote:
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
***************************************************************************************

Daniel L. Geiger, Ph.D.
Invertebrate Zoology
Santa Barbara Museum of Natural History
2559 Puesta del Sol Road
Santa Barbara, CA 93105

voice (805) 682 4711 x152

e-mail geiger-at-vetigastropoda.com
www http://www.vetigastropoda.com


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 14:06:13 2005



From: Anjeanette Ormonde :      Anjeanette.Ormonde-at-unilever.com
Date: Fri, 4 Mar 2005 15:12:52 -0500
Subject: [Microscopy] Metal Corrosion Reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Can anybody recommend a good reference that will help me figure out what
different types of corrosion look like? In the past I've been given samples of
steel containers where corrosion was suspected and was able to identify it
using EDS. This time there is a layer of steel coated with a thin layer of
tin. After storage with a solution in it a portion of the can is slightly
discolored. I was asked if I could determine if the discoloration is due to
detinning. I can't polish the samples flat but I did get a strong signal for
Tin using EDS so I'm convinced the layer is still there. But, I realize that I
do not know what to look for to determine if it is some sort of tin corrosion.
Any advice you can give on this problem would be greatly appreciated.
In addition, I would like to get a good reference so the next time I'm
presented with a new type of corrosion I can use it to help me figure out what
is going on.

Thanks,
Angie


Anjeanette Ormonde
Sr. Project Scientist - Unilever HPC
Rolling Meadows, IL 60008
Ph: 847-734-3539


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 4 14:22:29 2005



From: gay.ribisi-at-ns.microscopy.com (by way of MicroscopyListserver)
Date: Fri, 4 Mar 2005 18:09:14 -0600
Subject: [Microscopy] viaWWW: microscope with camera attachment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jay,

I also had the surgery in 2001 and would do it all again too. Money well
spent!

Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com



----- Original Message -----
} From: "Franklin Bailey" {jfb-at-uidaho.edu}
To: "Jay Campbell" {microtomy-at-gmail.com} ; {Microscopy-at-microscopy.com}
Sent: Friday, March 04, 2005 11:20 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (gay ribisi) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, March 4, 2005 at 13:42:01
---------------------------------------------------------------------------

Email: gay ribisi
Name: Gay Ribisi

Organization: artist

Title-Subject: [Microscopy] [Filtered] MListserver: microscope with camera attachment

Question:
I am trying to find a low cost dissecting stereomicroscope with a camera port to attach my Nikon f100 or Nikon D100. Any suggestions?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Mar 5 09:51:22 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Sat, 5 Mar 2005 07:58:29 -0800 (PST)
Subject: [Microscopy] Re: Metal Corrosion Reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I can't think of a reference that would help you in
this case, but I do have a few thoughts I'd like to
pass on.

Firstly, by using EDS methods you can determine if
there is a general loss of Tin on the surface by
looking at the Iron peak. Using a control sample
(either a new container, or better yet, an intact
surface from the same container), and assuming the tin
layer is thin enough that you can pick up iron from
underneath the tin (you may need to go to max kV to
accomplish this), you may see a significant difference
in Iron peak height which would correspond to a
difference in tin layer thickness. Naturally, you'll
want to use the smallest magnification to sample the
largest area for good statistical sampling.

Don't you see any other peaks in the stained area,
when compared with an unstained area (such as Oxygen
or Sulfur)? These other peaks should give you an idea
of what is happening to the stained area.

Secondly, if you have access to an X-ray
diffractometer, you may be able to use powder
diffraction techniques to identify the compounds on
the stained surface. Again, you should run a control
sample (unstained area) for comparison.

Lastly, the stained area can be characterized in a
cross section perpendicular to the surface by using
metallographic methods. To examine thin layers on the
surface, it is common to first electrolytically coat
the sample with nickel to avoid the edge rounding
effect that is common in metallographic samples.
Polishing by conventional metallographic methods would
then give a relatively true and flat view of the tin
layer. The methods would need to be somwhat modified
to prevent corrosion or staining of the cross section
- methods such as avoiding water after 600-grit
polish. Struers has a nice writeup on looking at zinc
layers on steel which would apply in your situation.

Regards,

Stu Smalinskas, P.E.
Sr. Metallurgist
SKF
Plymouth, Michigan
734-414-6862

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Angie wrote:

Can anybody recommend a good reference that will help
me figure out what different types of corrosion look
like? In the past I've been given samples of steel
containers where corrosion was suspected and was able
to identify it using EDS. This time there is a layer
of steel coated with a thin layer of tin. After
storage with a solution in it a portion of the can is
slightly discolored. I was asked if I could determine
if the discoloration is due to detinning. I can't
polish the samples flat but I did get a strong
signal for Tin using EDS so I'm convinced the layer is
still there. But, I realize that I do not know what
to look for to determine if it is some sort of tin
corrosion.

Any advice you can give on this problem would be
greatly appreciated.

In addition, I would like to get a good reference so
the next time I'm presented with a new type of
corrosion I can use it to help me figure out what
is going on.

Thanks,
Angie


Anjeanette Ormonde
Sr. Project Scientist - Unilever HPC
Rolling Meadows, IL 60008
Ph: 847-734-3539




__________________________________
Celebrate Yahoo!'s 10th Birthday!
Yahoo! Netrospective: 100 Moments of the Web
http://birthday.yahoo.com/netrospective/


From MicroscopyL-request-at-ns.microscopy.com Sat Mar 5 10:04:25 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 05 Mar 2005 11:11:25 -0500
Subject: [Microscopy] Secador Desiccator Cabinets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Michael Shaffer has written:
===================================================================
Is anyone familiar with the automatic dessicating cabinets available ... e.g
, "SECADOR" cabinets which are available from Electron Microscopy Sciences.
How do they work? More importantly, how well do they work?
===================================================================
The Secador™ family of desiccator cabinets are explained in detail (as well
as their operation) on the SPI Supplies website at URL
http://www.2spi.com/catalog/supp/desiccator-cabinets-secador.html

To put it simply, the cabinets have an automatic desiccant regenerating
station. It goes through an automatic cycle where by every so often (about
twenty minutes), the regenerating station is closed off from the volume of
the cabinet, the desiccant is heated, and the moisture is "blown off" to the
outside. After the heat cycle, the doors to the outside are closed, and the
doors to the inside of the chamber are opened, and the process of cabinet
desiccation started all over again.

SPI Supplies has sold these cabinets to a number of customers working not
only in microscopy laboratories but into other settings where one would
prefer to not have to handle particle-generating desiccants.

How well do they work? They work as as well as any other desiccator
cabinet but they work "better" if you factor in the fact that one does not
have to rely on remembering to change desiccant to keep the contents of the
chamber dry.

Disclaimer: SPI Supplies is a distributor for the Secador line of
desiccating cabinets.

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask
that any reply to this message be by way of the "reply" feature on your
software, so that the entire string of correspondence can come back to us
and all be in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Sat Mar 5 11:50:51 2005



From: Jian-Guo Zheng :      j-zheng3-at-northwestern.edu
Date: Sat, 5 Mar 2005 11:57:59 -0600
Subject: [Microscopy] Pros and cons of in-column and post-column energy filter in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
We know some pros and cons of in-column and post-column energy filters
in TEM. To summarize them, I would like to hear from you who have
experiences with the filters.
Thanks, Jian



From MicroscopyL-request-at-ns.microscopy.com Sat Mar 5 12:05:08 2005



From: Ronald Anderson :      randerson20-at-tampabay.rr.com
Date: Sat, 05 Mar 2005 13:11:56 -0500
Subject: [Microscopy] Microscopy Today March 2005 Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the March 2005 Microscopy Today table of contents. I will close
the subscription list for this issue on Thursday March 10, 2005.

Microscopists in North America and MSA members anywhere can subscribe
for free. Anyone else may subscribe for US$35 per year (to PARTIALLY
cover postage). All subscriptions at http://www.microscopy-today.com
Thank you.


Turning Blood Cells into Oyster Shells
Stephen W. Carmichael, Mayo Clinic

New Developments in GEMINI® FESEM Technology
H. Jaksch, J-P Vermeulen, Carl Zeiss SMT Oberkochen, Germany

Crystal Scanner for Nano-Metrology Applications
Paul West, Zhiqiang Peng, Natalia Starostina, Pacific Nanotechnology, Inc.

Towards Optimal Imaging and Microanalysis in Variable Pressure and Low
Voltage SEM
Brendan J Griffin, The University of Western Australia

Effects of Abbe Condenser Spherical Aberration on Image Quality
Theodore M. Clarke, Metallurgical Failure Analysis Consultant

Experience with a Dicing Saw for Rapid Pre-FIB TEM Sample Preparation
Jim Conner*, James Beck*, and Bryan Tracy,** *Freescale Semiconductor,
Crolles, France and Austin, TX, **Spansion, Sunnyvale, CA

Digital Scanning, Archiving, and Transmitting Electron Micrographs
Joiner Cartwright, Jr., Baylor College of Medicine

What TEM Camera Should I Choose?
Michael Bode, Michael Wibbelt,* and Christoph Huelk*
Soft Imaging system Corp. *Soft Imaging System GmbH

There is Art in Science and Science in Art
Thomas H. Saunders, Amateur Microscopist

Sample Preparation for Textile Nanofiber Composites
R. Garcia, N. Fedorova, V. Knowlton, C. Oldham, B. Pourdeyhimi, NC State
University, Raleigh

Funding Opportunities for Acquiring Major Equipment from Federal
Granting Agencies M&M 2004 Core Facility Management – Part II: NSF
Organizer: Debby Sherman, Purdue University

New Resin for Repair of Bell Jar Chips
Owen P. Mills and Matt Huuki,* Michigan Tech. Univ., * Matt’s Auto
Glass, Houghton, MI

Inexpensive Digitization of an SEM
Henry C. Aldrich and Donna S. Williams, Univ. of Florida, Gainesville

Industry News

NetNotes



Ron Anderson, Editor
Microscopy Today



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 02:34:19 2005



From: Evren Cubukcu :      ecubukcu-at-hacettepe.edu.tr
Date: Mon, 07 Mar 2005 10:44:23 +0200
Subject: [Microscopy] RE: SEM - LEO or JEOL?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you very much for such valuable comments and suggestions.
I have been in SEM field for 2 years now and your contributions are really
amazing.

Evren




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 10:41:47 2005



From: Ian MacLaren :      i.maclaren-at-physics.gla.ac.uk
Date: Mon, 07 Mar 2005 16:50:28 +0000
Subject: [Microscopy] Re: Pros and cons of in-column and post-column energy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,
I'm sure there are better experts than me out there, but I just have a
few comments from my own experience.

Post column:
Pros:
can be attached to almost any TEM
Can be fairly easy to operate with proprietary software from manufacturer
Con:
Can have large post-column mag
- restricts field of view, cannot do low mag work (but see next two
points)
- partially fixed in newer models
- can be corrected by demagnifying image in projection lenses of
microscope, when this is set up correctly, easier on some microscopes
than others
Integration in/with microscope software - may vary with microscope
manufacturer - relies on good relations between the two manufacturers
Cannot use with film, must use CCD

In-column
Pros:
Can photograph image to film or CCD as you choose
What you see is what you get from fluorescent screen to film/CCD
No extra magnification - can do high or low mag, as you will (easier
then for energy filtered diffraction)
Should integrate perfectly into microscope software since microscope and
filter from same manufacturer
Cons:
Only available from some manufacturers (mainly LEO and JEOL)
Can be rather expensive
Limited choice of high voltages
Depends if you like the manufacturers proprietary software

Best wishes

--
Ian MacLaren
Department of Physics and Astronomy
University of Glasgow
Glasgow G12 8QQ
Scotland
http://www.ssp.gla.ac.uk/

****************************************************
FERROELECTRICS UK 2005: 26-27th April 2005
http://www.paisley.ac.uk/computing/ferroelectrics/
****************************************************



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 12:19:48 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 7 Mar 2005 10:44:09 -0800
Subject: [Microscopy] Re: Pros and cons of in-column and post-column energy filter in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 5, 2005, at 9:57 AM, Jian-Guo Zheng wrote:

} We know some pros and cons of in-column and post-column energy filters
} in TEM. To summarize them, I would like to hear from you who have
} experiences with the filters.
}
Dear Jian,
We have a Gatan post-column imaging energy filter, which we use
exclusively for imaging of biological cryo-specimens. The images
obtained on this system are of very high quality. I have no experience
using the system for EELS or element mapping, nor do I have any
experience with in-column filters.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 12:31:02 2005



From: Mike Zemyan :      mzemyan-at-schaferlabs.com
Date: Mon, 7 Mar 2005 10:41:06 -0800
Subject: [Microscopy] RE: Secador Desiccator Cabinets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In reading the description of these automatic dessicators, I notice that
they have 2 fans, one of which continually circulates air inside the
chamber. Does anyone know, are the fan bearings oil-lubricated? Is this
potentially a source of oil vapor contamination? We use dessicators (not
the automatic kind) to store cleaned vacuum parts, and so oil is a concern.

Michael Zemyan
Schafer Corporation

} -----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
} Sent: Saturday, March 05, 2005 8:11 AM
} To: MICROSCOPY BB
} Subject: [Microscopy] Secador Desiccator Cabinets
}
}
}
} --------------------------------------------------------------
} ----------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} -----------------
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Michael Shaffer has written:
} ===================================================================
} Is anyone familiar with the automatic dessicating cabinets
} available ... e.g , "SECADOR" cabinets which are available
} from Electron Microscopy Sciences.
} How do they work? More importantly, how well do they work?
} ===================================================================
} The SecadorT family of desiccator cabinets are explained in
} detail (as well as their operation) on the SPI Supplies
} website at URL
} http://www.2spi.com/catalog/supp/desiccator-cabinets-secador.html
}
} To put it simply, the cabinets have an automatic desiccant
} regenerating station. It goes through an automatic cycle
} where by every so often (about twenty minutes), the
} regenerating station is closed off from the volume of the
} cabinet, the desiccant is heated, and the moisture is "blown
} off" to the outside. After the heat cycle, the doors to the
} outside are closed, and the doors to the inside of the
} chamber are opened, and the process of cabinet desiccation
} started all over again.
}
} SPI Supplies has sold these cabinets to a number of customers
} working not only in microscopy laboratories but into other
} settings where one would
} prefer to not have to handle particle-generating desiccants.
}
} How well do they work? They work as as well as any other
} desiccator cabinet but they work "better" if you factor in
} the fact that one does not have to rely on remembering to
} change desiccant to keep the contents of the chamber dry.
}
} Disclaimer: SPI Supplies is a distributor for the Secador
} line of desiccating cabinets.
}
} Chuck
}
} PS: Please remember that we are nearly 100% paperless and
} we would ask
} that any reply to this message be by way of the "reply"
} feature on your software, so that the entire string of
} correspondence can come back to us and all be in one place.
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================
}
}



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 12:50:33 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Mon, 7 Mar 2005 10:59:45 -0800 (PST)
Subject: [Microscopy] Re: Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i suspect those are intracelluar viruse particles.
more info on the tissue ie. is it a path speicimen
would be helpful.
john
--- Debby Sherman {dsherman-at-purdue.edu} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} I'll take a stab at it and say it looks like virus
} in a crystalline array
} although size appears a bit small.
}
} Is there any signs of fibers in longitudinal
} orientation? If it is made up
} of cytoskeletal elements, i.e. fibers of different
} diameters, than I would
} expect to see some in longitudinal as well as
} x-sectional view.
}
} Debby
}
} Debby Sherman, Manager Phone:
} 765-494-6666
} Life Science Microscopy Facility FAX:
} 765-494-5896
} Purdue University E-mail:
} dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
} On 3/3/05 12:38 PM, "Aleksandr Mironov"
} {Aleksandr.Mironov-at-manchester.ac.uk}
} wrote:
}
} }
} }
} }
}
------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
------------------------------------------------------------------------------}
} -
} }
} } Dear collegues!
} }
} } Just recently I found the structure, which I
} cannot identify. It has
} } regular hexagonal pattern and localized in the
} cytosol of the cells in
} } embryonic tendons. It is not membrabous, some kind
} of cytoskeletal?
} }
} } Could you, please, take a look and hint what it
} could be?
} } The address for pictures is (structure is
} identified by arrows):
} } http://sysoj.spymac.net/image.jpg
} } http://sysoj.spymac.net/image2.jpg
} }
} } Regards,
}
}
}
}

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 14:25:31 2005



From: David Hall :      hall-at-aecom.yu.edu
Date: Mon, 7 Mar 2005 15:35:22 -0500
Subject: [Microscopy] Re: TEM Identifying intracellular structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would vote for these to be an actin/myosin myofilament lattice.
The smaller actin fibrils surround each myosin rod in typical
fashion. The only surprising aspect is that the lattice is seen in
perfect cross-section and each separate portion of lattice seems to
be co-linear, suggesting that the whole cell is already polarized and
becoming anchored.

We've recently put up some images of nematode muscle that show these
features in comparison to vertebrate muscle: see Fig's 11, 15, 35
and 36 at the following two webpages:
www.wormatlas.org/handbook/mesodermal.htm/musclepartI.htm
www.wormatlas.org/handbook/mesodermal.htm/musclepartII.htm
--
David H. Hall, Ph.D.
Center for C. elegans Anatomy
Department of Neuroscience
Albert Einstein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461

www.wormatlas.org
www.aecom.yu.edu/wormem

phone 718 430-2195
fax 718 430-2514


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 14:26:23 2005



From: Chaoying Ni :      cni-at-udel.edu
Date: Mon, 7 Mar 2005 15:41:02 -0500
Subject: [Microscopy] Re: Pros and cons of in-column and post-column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Then Bill, in your case, GIF = CCD with a limited magnification range
and other inconveniences.

-Chao


-----Original Message-----
} From: Bill Tivol [mailto:tivol-at-caltech.edu]
Sent: Monday, March 07, 2005 1:44 PM
To: microscopy-at-msa.microscopy.com

On Mar 5, 2005, at 9:57 AM, Jian-Guo Zheng wrote:

} We know some pros and cons of in-column and post-column energy filters
} in TEM. To summarize them, I would like to hear from you who have
} experiences with the filters.
}
Dear Jian,
We have a Gatan post-column imaging energy filter, which we use
exclusively for imaging of biological cryo-specimens. The images
obtained on this system are of very high quality. I have no experience
using the system for EELS or element mapping, nor do I have any
experience with in-column filters.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 14:33:01 2005



From: Chaoying Ni :      cni-at-udel.edu
Date: Mon, 7 Mar 2005 15:47:03 -0500
Subject: [Microscopy] Re: Pros and cons of in-column and post-column

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jian,

Ian made quite nice points. Is it also true that post-filter is better
for spectroscopy while in-column filter is better for energy-filtered
imaging? But I'm not so sure.
-Chao

-----Original Message-----
} From: Ian MacLaren [mailto:i.maclaren-at-physics.gla.ac.uk]
Sent: Monday, March 07, 2005 11:50 AM
To: Jian-Guo Zheng
Cc: Microscopy-at-microscopy.com

Dear all,
I'm sure there are better experts than me out there, but I just have a
few comments from my own experience.

Post column:
Pros:
can be attached to almost any TEM
Can be fairly easy to operate with proprietary software from
manufacturer
Con:
Can have large post-column mag
- restricts field of view, cannot do low mag work (but see next two
points)
- partially fixed in newer models
- can be corrected by demagnifying image in projection lenses of
microscope, when this is set up correctly, easier on some microscopes
than others
Integration in/with microscope software - may vary with microscope
manufacturer - relies on good relations between the two manufacturers
Cannot use with film, must use CCD

In-column
Pros:
Can photograph image to film or CCD as you choose
What you see is what you get from fluorescent screen to film/CCD
No extra magnification - can do high or low mag, as you will (easier
then for energy filtered diffraction)
Should integrate perfectly into microscope software since microscope and

filter from same manufacturer
Cons:
Only available from some manufacturers (mainly LEO and JEOL)
Can be rather expensive
Limited choice of high voltages
Depends if you like the manufacturers proprietary software

Best wishes

--
Ian MacLaren
Department of Physics and Astronomy
University of Glasgow
Glasgow G12 8QQ
Scotland
http://www.ssp.gla.ac.uk/

****************************************************
FERROELECTRICS UK 2005: 26-27th April 2005
http://www.paisley.ac.uk/computing/ferroelectrics/
****************************************************





From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 14:36:21 2005



From: Leslie Cummins :      gunther-at-aecom.yu.edu
Date: Mon, 07 Mar 2005 15:46:16 -0500
Subject: [Microscopy] Transfer of fungal cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers

We have a user that has some fungi attached to stone chips from Italian
monuments. He needs to get the fungi off the chips and onto a slide, so he
can do some fluorescence.

We have a few ideas to transfer the cells. First, to physically scrape
them off onto the slide. Second, transfer them using some sort of tape,
like plain old Scotch. But then, how do we get them to stick to the
slide. We are concerned that poly-lysine might not hold them down through
all his changes. How do we get them off the tape and onto the slide. How
do we try to keep the morphology as intact as possible?

If anyone has any ideas, we would love to hear your input.

Thanks in advance.
Leslie

Leslie Cummins www.aecom.yu.edu/aif
Analytical Imaging Facility
1300 Morris Park Ave
Forchheimer 639
Bronx, NY 10461
718-430-3547



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 14:39:34 2005



From: Becky Holdford :      r-holdford-at-ti.com
Date: Mon, 07 Mar 2005 14:48:46 -0600
Subject: [Microscopy] Re: Metal Corrosion Reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anjeanette: this might be a good reference from ASM:
http://www.asminternational.org/Template.cfm?Section=BrowsebyTopic&template=Ecommerce/ProductDisplay.cfm&ProductID=10488
This is the ASM Handbook Volume 13: Corrosion.
Another good one might be "Corrosion: Understanding the Basics" from the
same place.

Anjeanette Ormonde wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 16:44:52 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 07 Mar 2005 14:54:33 -0800
Subject: [Microscopy] RE: SEM - LEO or JEOL?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


First of all, what method did you use to arrive
at the selection of an EVO SEM? Why not high vacuum?
I think that this needs to be factored if you're not sure.
What type of final specimen are you going to be analyzing?
If the soil is embedded, polished low mag imaged, you don't
necessarily need an ESEM. That said, if this is mostly all you
will be looking at, you can suffer the loss of resolution
of an ESEM. That you prefer not needing a chiller
is important. However, there are many advantages
in SEM systems that are only achieved by chiller-based
SEMs. You need to sort out all of the variables
for a SEM system first, I think, then select an
appropriate EDS system. In both cases, availability
of service resources is critical. If you don't have
a chiller, what cools the diffusion pump or turbo?
Some turbos can be air cooled.

If you are going to do an ESEM, it may likely
have a W filament or LaB6 at best. Thus, how
are you going to achieve 400K cps much less than
actually processing them? Furthermore, regardless
of the maximum cps stated, what filter time is this based on?
Short times can handle large cps at low resolution
and low fidelity. As filter time increases, dead time
increases and the existence of many counts becomes
irrellevant if not counterproductive. Most (my guess)
systems run about 2000 cps at 30% DT at 102uS time
constant. If you are concerned about resolution and
sensitivity, you should focus on obtaining long filter
time and { 35% DT. A system can certainly handle
hundreds of thousands cps but not likely at long filter
times. In this case, short filter times don't produce
good quant results. However, short times are valuable for
mapping. So, keep this in mind based on your application.
If you seek high resolution, you need a very good detector
and long filter times. Additionally, it seems out of
character to me that a W system would produce very high
counts compared to a LaB6 or especially an FESEM. But
I've only done EDS on FESEMs.

The other issue to keep in mind is that if you want to
do quant EDS, you will need an EDS package that allows
for high pressure quant. I use EDAX Genesis VIP Quant
for variable pressure SEM (usually 5-50Pa). It is selectable
for VP or high vacuum. Furthermore, it provides for
ZAF, PhiZAF and PhiRhoZAF quant in VP or HV modes.
The user interface is very nice, IMO. I have used the
Rontec as well and got good results in a high vacuum
system. I don't know if they can handle high pressure
situations.

There have been discussions about standardless quant
on this list before. The method I use is what EDAX calls
SEC--a standards correction factor. This is where the
quant value is typically value*SEC where SEC=1. However,
the SEC value can be changed to a value that makes the
quant result match a standard. Thus, quant results are
done routinely in a standardless manner but are traced
back to a standard. This SEC check should be done on
a routine basis (I do it every quarter). The other thing
to check is that the peak for each detected element lines
up with its respective eV for whichever shell is being
examined. This value can shift slightly. Sometimes,
an element's eV value might be high or low by a small
amount and confuse a convolution (peak pileup).

As far as the detector being LN2-free, I agree very much.
The tradeoffs for this option are cost and performance.
Without getting at or close to LN2 temperature, detector
performance will suffer. I use the EDAX Cryospec LN2-free
detector. This detector produces the same performance
as an LN2 detector. Currently, I am getting 131.5eV
resolution and can detect down to and including Be.
But of course, YMMV.

gary g.

At 12:44 AM 3/7/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 17:06:07 2005



From: Tamara Howard :      thoward-at-unm.edu
Date: Mon, 7 Mar 2005 16:15:54 -0700 (MST)
Subject: [Microscopy] Re: Transfer of fungal cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Double-sided scotch tape?

On Mon, 7 Mar 2005, Leslie Cummins wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hello Listers
}
} We have a user that has some fungi attached to stone chips from Italian
} monuments. He needs to get the fungi off the chips and onto a slide, so he
} can do some fluorescence.
}
} We have a few ideas to transfer the cells. First, to physically scrape them
} off onto the slide. Second, transfer them using some sort of tape, like
} plain old Scotch. But then, how do we get them to stick to the slide. We
} are concerned that poly-lysine might not hold them down through all his
} changes. How do we get them off the tape and onto the slide. How do we try
} to keep the morphology as intact as possible?
}
} If anyone has any ideas, we would love to hear your input.
}
} Thanks in advance.
} Leslie
}
} Leslie Cummins www.aecom.yu.edu/aif
} Analytical Imaging Facility
} 1300 Morris Park Ave
} Forchheimer 639
} Bronx, NY 10461
} 718-430-3547
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 17:28:42 2005



From: kak-at-medicine.wisc.edu (by way of Ask-A-Microscopist)
Date: Mon, 7 Mar 2005 17:39:09 -0600
Subject: [Microscopy] viaWWW: binocular compound microscope as a hobbyist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kak-at-medicine.wisc.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, March 7, 2005 at 15:52:14
---------------------------------------------------------------------------

Email: kak-at-medicine.wisc.edu
Name: Kip Kircher

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I am researching the purchase of a binocular compound microscope as a hobbyist. The LOMO BMH4-BF seems the best deal at $445. I find it ugly however and am suspicious of the quality. Is this the best under $500 deal available?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 17:51:48 2005



From: Angela V. Klaus :      avklaus-at-amnh.org
Date: Mon, 7 Mar 2005 19:00:29 -0500 (EST)
Subject: [Microscopy] LN2-free x-ray detectors

Contents Retrieved from Microscopy Listserver Archives
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Hi Gary,

I'm thinking about switching to an LN2-free EDS detector. It sounds like
you're fairly happy with yours, but I was wondering what "YMMV" stands for
in the below portion of your note:

} As far as the detector being LN2-free, I agree very much.
} The tradeoffs for this option are cost and performance.
} Without getting at or close to LN2 temperature, detector
} performance will suffer. I use the EDAX Cryospec LN2-free
} detector. This detector produces the same performance
} as an LN2 detector. Currently, I am getting 131.5eV
} resolution and can detect down to and including Be.
} But of course, YMMV.

I'd be really interested to hear what other experiences people have had
with liquid nitrogen-free EDS detectors.

All best,

Angela

--
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-796-5977
Fax: 212-496-3480

}
}
} ------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America








From MicroscopyL-request-at-ns.microscopy.com Mon Mar 7 20:27:19 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 07 Mar 2005 18:37:32 -0800
Subject: [Microscopy] Re: LN2-free x-ray detectors

Contents Retrieved from Microscopy Listserver Archives
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Reply made off-list.

gary g.



At 04:00 PM 3/7/2005, you wrote:


} ------------------------------------------------------------------------------
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From MicroscopyL-request-at-ns.microscopy.com Tue Mar 8 09:20:35 2005



From: Peter Van Osta :      pvosta-at-maia-scientific.com
Date: Tue, 08 Mar 2005 16:30:02 +0100
Subject: [Microscopy] Filter for DRAQ5 and GFP

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Which filterset (Omega, ...) and dichroic is mostly used for dual label
experiments with the nuclear probe DRAQ5 and a GFP label ?

Regards,

Peter Van Osta

----------------------------------------------
Peter Van Osta, MD

Director Imaging
MAIA SCIENTIFIC
Cipalstraat 3
B-2440 Geel, Belgium
Tel.: +32 (0)14 570 620
Mobile: +32 (0)497 228 725
Fax.: +32 (0)14 570 621
Email: pvosta-at-maia-scientific.com
Website: www.maia-scientific.com
A Harvard Bioscience Company
----------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 8 10:19:14 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 08 Mar 2005 08:28:42 -0800
Subject: [Microscopy] Re: LN2-free x-ray detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, I suppose for hassle reduction purposes.
It seems that whenever products are discussed
rather than procedures, the thread can turn into
a contrarian epic. I'm just trying to answer the
question based on what I have, without speculation
about what something else might do.

Here is the response:

/# begin #/
I'm fairly happy with mine. This is the third
unit I have had of the Cryospec. The system
performs extraordinarily well when all is stable
and nice. What the system hates is being turned off
for awhile and then turned back on. In this case,
it may not achieve low enough temperature for the
HV to turn on--dead system.

The Cryospec uses essentially the same basic Si(Li)
Moxtec detector as any EDAX (or other) LN2 detector.
The difference is that there is a cryo pump which
is a separate box with light weight hoses to a
screwed-on unit for the detector that cools the
detector. The cryo pump is made by MMR and does a
very good job when stable. It is consistent and
dependable. The resolution of the Cryospec detector
is excellent and does this without LN2. It just sits
and runs. I really like the EDAX Genesis software
package. Easy to use and their HPD feature is great
for identifying wrong element peaks.

If you get the mapping option and image collection
option, you will then have a digital image capture
SEM. Plus, you can do maps based on detected Zs or
whatever you want. And there are many other features
of the Genesis package. I also have a remote for my
separate PC and notebook PC so I can analyze EDS
spectra separate from the SEM system. Very handy.
/# end #/

The cooling versus non-cooling was intended to
address SEM units only. Cooled versus non-cooled can
have a big impact on what is sitting in your room (foot print)
and what image results and performance you obtain.
Specs are one thing but actually achieving them is
another. An un-coated specimen imaged at 100V, 4mm WD
and 150KX would, I think, be rather difficult without
a cooled SEM. And the design of the SEM would
have to be quite different from traditional designs.

Zeiss achieves this in high vacuum mode on Supra and
Ultra FESEMs. I don't know about their EVSEM performance.
That was the crux of the suggestion of matching a SEM to
requirements rather than stating the SEM type up front.

gary g.


At 05:18 AM 3/8/2005, you wrote:
} Gary,
}
} Just curious as to why you chose not to share the reply. I am also
} interested in learning pros and cons about N2-free detectors.
} In your E-mail below you seem to indicate that not cooling may have negative
} affects on performance as well as increased costs. Then you state that your
} N2-free detector works equivalent to those requiring cooling.
}
} Could you explain this apparent contradiction?
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
} On 3/7/05 9:37 PM, "Gary Gaugler" {gary-at-gaugler.com} wrote:
}
} }
} }
} }
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ------------------------------------------------------------------------------}
} -
} }
} } Reply made off-list.
} }
} } gary g.
} }
} }
} }
} } At 04:00 PM 3/7/2005, you wrote:
} }
} }
} } }
} -----------------------------------------------------------------------------} }
} -
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------------
} } } --
} } }
} } } Hi Gary,
} } }
} } } I'm thinking about switching to an LN2-free EDS detector. It sounds like
} } } you're fairly happy with yours, but I was wondering what "YMMV" stands for
} } } in the below portion of your note:
} } }
} } } } As far as the detector being LN2-free, I agree very much.
} } } } The tradeoffs for this option are cost and performance.
} } } } Without getting at or close to LN2 temperature, detector
} } } } performance will suffer. I use the EDAX Cryospec LN2-free
} } } } detector. This detector produces the same performance
} } } } as an LN2 detector. Currently, I am getting 131.5eV
} } } } resolution and can detect down to and including Be.
} } } } But of course, YMMV.
} } }
} } } I'd be really interested to hear what other experiences people have had
} } } with liquid nitrogen-free EDS detectors.
} } }
} } } All best,
} } }
} } } Angela
} } }
} } } --
} } } Angela V. Klaus, Ph.D.
} } } Director, Microscopy and Imaging Facility
} } } American Museum of Natural History
} } } Central Park West and 79th Street
} } } New York, NY 10024 USA
} } } Email: avklaus-at-amnh.org
} } } Tel: 212-796-5977
} } } Fax: 212-496-3480
} } }
} } } }
} } } }
} } } }
} } }
} -----------------------------------------------------------------------------} }
} -
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------------
} } } --
} } } }
} } } }
} } } } First of all, what method did you use to arrive
} } } } at the selection of an EVO SEM? Why not high vacuum?
} } } } I think that this needs to be factored if you're not sure.
} } } } What type of final specimen are you going to be analyzing?
} } } } If the soil is embedded, polished low mag imaged, you don't
} } } } necessarily need an ESEM. That said, if this is mostly all you
} } } } will be looking at, you can suffer the loss of resolution
} } } } of an ESEM. That you prefer not needing a chiller
} } } } is important. However, there are many advantages
} } } } in SEM systems that are only achieved by chiller-based
} } } } SEMs. You need to sort out all of the variables
} } } } for a SEM system first, I think, then select an
} } } } appropriate EDS system. In both cases, availability
} } } } of service resources is critical. If you don't have
} } } } a chiller, what cools the diffusion pump or turbo?
} } } } Some turbos can be air cooled.
} } } }
} } } } If you are going to do an ESEM, it may likely
} } } } have a W filament or LaB6 at best. Thus, how
} } } } are you going to achieve 400K cps much less than
} } } } actually processing them? Furthermore, regardless
} } } } of the maximum cps stated, what filter time is this based on?
} } } } Short times can handle large cps at low resolution
} } } } and low fidelity. As filter time increases, dead time
} } } } increases and the existence of many counts becomes
} } } } irrellevant if not counterproductive. Most (my guess)
} } } } systems run about 2000 cps at 30% DT at 102uS time
} } } } constant. If you are concerned about resolution and
} } } } sensitivity, you should focus on obtaining long filter
} } } } time and { 35% DT. A system can certainly handle
} } } } hundreds of thousands cps but not likely at long filter
} } } } times. In this case, short filter times don't produce
} } } } good quant results. However, short times are valuable for
} } } } mapping. So, keep this in mind based on your application.
} } } } If you seek high resolution, you need a very good detector
} } } } and long filter times. Additionally, it seems out of
} } } } character to me that a W system would produce very high
} } } } counts compared to a LaB6 or especially an FESEM. But
} } } } I've only done EDS on FESEMs.
} } } }
} } } } The other issue to keep in mind is that if you want to
} } } } do quant EDS, you will need an EDS package that allows
} } } } for high pressure quant. I use EDAX Genesis VIP Quant
} } } } for variable pressure SEM (usually 5-50Pa). It is selectable
} } } } for VP or high vacuum. Furthermore, it provides for
} } } } ZAF, PhiZAF and PhiRhoZAF quant in VP or HV modes.
} } } } The user interface is very nice, IMO. I have used the
} } } } Rontec as well and got good results in a high vacuum
} } } } system. I don't know if they can handle high pressure
} } } } situations.
} } } }
} } } } There have been discussions about standardless quant
} } } } on this list before. The method I use is what EDAX calls
} } } } SEC--a standards correction factor. This is where the
} } } } quant value is typically value*SEC where SEC=1. However,
} } } } the SEC value can be changed to a value that makes the
} } } } quant result match a standard. Thus, quant results are
} } } } done routinely in a standardless manner but are traced
} } } } back to a standard. This SEC check should be done on
} } } } a routine basis (I do it every quarter). The other thing
} } } } to check is that the peak for each detected element lines
} } } } up with its respective eV for whichever shell is being
} } } } examined. This value can shift slightly. Sometimes,
} } } } an element's eV value might be high or low by a small
} } } } amount and confuse a convolution (peak pileup).
} } } }
} } } } As far as the detector being LN2-free, I agree very much.
} } } } The tradeoffs for this option are cost and performance.
} } } } Without getting at or close to LN2 temperature, detector
} } } } performance will suffer. I use the EDAX Cryospec LN2-free
} } } } detector. This detector produces the same performance
} } } } as an LN2 detector. Currently, I am getting 131.5eV
} } } } resolution and can detect down to and including Be.
} } } } But of course, YMMV.
} } } }
} } } } gary g.
} } } }
} } } } At 12:44 AM 3/7/2005, you wrote:
} } } }
} } } }
} } } } } ------------------------------------------------------------------------
} } } ------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } } } http://www.microscopy.com/MicroscopyListserver
} } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} -----------------------------------------------------------------------------
} } } --
} } } Thank you very much for such valuable comments and suggestions.
} } } } } I have been in SEM field for 2 years now and your contributions are
} } } } } really
} } } } } amazing.
} } } } } Evren
} } } }
} } } }
} } } }
} }
} }



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 8 15:19:17 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 08 Mar 2005 13:28:55 -0800
Subject: [Microscopy] Re: RE: SEM - LEO or JEOL?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks. Yes. The point is that being able to handle
many counts only is relevant if the system will produce
them. With a W emitter, one would need high KV, big
aperture and relaxed condenser to get high counts.
Then, the result is poor resolution. My FESEM will
do up to at least 60K cps depending on KV, gun aperture
size, high or low current mode and Z of specimen. but
at high cps, in order to keep DT { 35%, filter time
is short. I'd rather have good resolution and just
take a little longer to get good quant data.

The EDAX Genesis has what they call VIP Quant for doing
variable pressure quant. It does like you guessed. One
takes a reading at one pressure then another at a different
pressure. It then extrapolates and produces the same type
of output as it does at high vacuum (HV or VP is selected
by a click box in the app).

when quant is produced, there is quant data along with
a separate listing of intensities for each element and
the intensity ratio error. If the ratio error is less
than about 10% or so, the result is considered OK. If
around 20% or greater, the result is bad--likely, wrong
element was tagged. So, go back and find out what the
correct element is close to the bad Z. this usually
does not happen since the HPD (halographic peak deconvolution)
shows if the user correctly identified elements. It also
shows if the user missed an element in a pile up of Zs.

This all works in high vacuum and VP modes. I'm running
between 20-50Pa...just enough to get rid of charging
with insulating specimens. Each one is slightly different.
If I recall correctly, VIP Quant also works for EVSEM (higher pressures),
not sure about this.

The way I do phase very nicely is with EDS coupled with EBSD.
Even so, with just EDS, Genesis will do whole scan measurements
or spot or ROI (option). This way, find an area and analyze it.
Then, do quant. If you want to know where stuff is, do a map
based on the collected Z list. But the best way is EDS+EBSD.

gary g.



At 10:13 AM 3/8/2005, you wrote:
} Nice comments about resolution and count rate. I don't think I've ever
} gotten more than 20 kcps out of our tungsten gun. FE would certainly make
} a difference.
}
} I am curious about your comments about high-pressure quant. Does EDAX have
} a special package for that? What does it do?
}
} I know that we get contributions from the neighboring area and have to be
} very careful when trying to do quant at high pressures (20-100 Pa). I was
} looking at gold-bearing minerals for a fellow last week. The gangue
} mineral was silicious so everything had silicon in it. Likewise, I get
} bogus Al-Cu analyses when I have a small Cu-rich phase dispersed in an
} Al-rich matrix. I can't quite imagine how they would handle the problem
} without a lot of knowledge about the specimen. Do they perform analyses
} under multiple pressures and extrapolate back to zero pressure?
}
} Curious Warren
}
} At 04:54 PM 03/07/05, you wrote:
}
} } First of all, what method did you use to arrive
} } at the selection of an EVO SEM? Why not high vacuum?
} } I think that this needs to be factored if you're not sure.
} } What type of final specimen are you going to be analyzing?
} } If the soil is embedded, polished low mag imaged, you don't
} } necessarily need an ESEM. That said, if this is mostly all you
} } will be looking at, you can suffer the loss of resolution
} } of an ESEM. That you prefer not needing a chiller
} } is important. However, there are many advantages
} } in SEM systems that are only achieved by chiller-based
} } SEMs. You need to sort out all of the variables
} } for a SEM system first, I think, then select an
} } appropriate EDS system. In both cases, availability
} } of service resources is critical. If you don't have
} } a chiller, what cools the diffusion pump or turbo?
} } Some turbos can be air cooled.
} }
} } If you are going to do an ESEM, it may likely
} } have a W filament or LaB6 at best. Thus, how
} } are you going to achieve 400K cps much less than
} } actually processing them? Furthermore, regardless
} } of the maximum cps stated, what filter time is this based on?
} } Short times can handle large cps at low resolution
} } and low fidelity. As filter time increases, dead time
} } increases and the existence of many counts becomes
} } irrellevant if not counterproductive. Most (my guess)
} } systems run about 2000 cps at 30% DT at 102uS time
} } constant. If you are concerned about resolution and
} } sensitivity, you should focus on obtaining long filter
} } time and { 35% DT. A system can certainly handle
} } hundreds of thousands cps but not likely at long filter
} } times. In this case, short filter times don't produce
} } good quant results. However, short times are valuable for
} } mapping. So, keep this in mind based on your application.
} } If you seek high resolution, you need a very good detector
} } and long filter times. Additionally, it seems out of
} } character to me that a W system would produce very high
} } counts compared to a LaB6 or especially an FESEM. But
} } I've only done EDS on FESEMs.
} }
} } The other issue to keep in mind is that if you want to
} } do quant EDS, you will need an EDS package that allows
} } for high pressure quant. I use EDAX Genesis VIP Quant
} } for variable pressure SEM (usually 5-50Pa). It is selectable
} } for VP or high vacuum. Furthermore, it provides for
} } ZAF, PhiZAF and PhiRhoZAF quant in VP or HV modes.
} } The user interface is very nice, IMO. I have used the
} } Rontec as well and got good results in a high vacuum
} } system. I don't know if they can handle high pressure
} } situations.
} }
} } There have been discussions about standardless quant
} } on this list before. The method I use is what EDAX calls
} } SEC--a standards correction factor. This is where the
} } quant value is typically value*SEC where SEC=1. However,
} } the SEC value can be changed to a value that makes the
} } quant result match a standard. Thus, quant results are
} } done routinely in a standardless manner but are traced
} } back to a standard. This SEC check should be done on
} } a routine basis (I do it every quarter). The other thing
} } to check is that the peak for each detected element lines
} } up with its respective eV for whichever shell is being
} } examined. This value can shift slightly. Sometimes,
} } an element's eV value might be high or low by a small
} } amount and confuse a convolution (peak pileup).
} }
} } As far as the detector being LN2-free, I agree very much.
} } The tradeoffs for this option are cost and performance.
} } Without getting at or close to LN2 temperature, detector
} } performance will suffer. I use the EDAX Cryospec LN2-free
} } detector. This detector produces the same performance
} } as an LN2 detector. Currently, I am getting 131.5eV
} } resolution and can detect down to and including Be.
} } But of course, YMMV.
} }
} } gary g.
}



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 8 16:17:29 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 08 Mar 2005 17:27:43 -0500
Subject: [Microscopy] Spelling question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Is there any opinion as to the preferred way of spelling:

Bremmstrahlung (948 hits)

or

Bremsstrahlung (317,000 hits)

radiation?

If one does Google searches on each of the two spelllings, there is indeed
indicated a preference of one over the other, as indicated above. Does that
mean the second spelling is the correct one?

Chuck


============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================





From MicroscopyL-request-at-ns.microscopy.com Tue Mar 8 18:06:09 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Tue, 8 Mar 2005 17:14:04 -0700
Subject: [Microscopy] Spelling question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Chuck,

the word is German and comes from "bremsen" (to break, slow down) and
"Strahlung" (radiation). The correct spelling would be "Bremsstrahlung".

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: Tuesday, March 08, 2005 15:28
To: MICROSCOPY BB

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Is there any opinion as to the preferred way of spelling:

Bremmstrahlung (948 hits)

or

Bremsstrahlung (317,000 hits)

radiation?

If one does Google searches on each of the two spelllings, there is indeed
indicated a preference of one over the other, as indicated above. Does that
mean the second spelling is the correct one?

Chuck


============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================






From MicroscopyL-request-at-ns.microscopy.com Tue Mar 8 18:25:35 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 8 Mar 2005 16:50:03 -0800
Subject: [Microscopy] Re: Spelling question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 8, 2005, at 2:27 PM, Garber, Charles A. wrote:

} Bremsstrahlung (317,000 hits)
}
Dear Chuck,
This spelling is correct; my limited memory of German is that "brems"
is from "braking", "strahl" means "radiate" or "stream" (the verb), and
the suffix "ung" makes the word a noun (like "ing"). Those on the list
who know more German than I can correct me.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 8 18:33:19 2005



From: Becky Holdford :      r-holdford-at-ti.com
Date: Tue, 08 Mar 2005 18:43:29 -0600
Subject: [Microscopy] Texas Society for Microscopy 40th Anniversary meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to take this opportunity to invite you to the 40th anniversary
celebration of the Texas Society for Microscopy. The spring meeting will
be held April 14-16 in Las Colinas, TX at the Dallas Marriott and marks a
milestone for our society. Below are some of the highlights for this meeting:

+ Parallel sessions for the biological sciences and materials sciences
+ Special Friday afternoon session to celebrate our history
+ Workshop " Low Voltage Variable Pressure SEM for Nanotechnology"
presented by Steve Joens at Hitachi
+ Guest speakers
- "Failure Analysis of Non Visual Fails on Integrated Circuits
Using Nano Probing in an SEM" presented by Dr. Richard Stallcup III, Zyvex
Corporation
- "Optical Coherence Microscopy, A Technology for Rapid, In Vivo,
Nondestructive Visualization of Plants and Plants Cells" presented by Dr.
June Medford, Biology Dept, Colorado State University
+ Vendor exhibits
+ Platform presentations and poster session
+ Photo contest

Additional information can be found at http://www.texasmicroscopy.org/

Hope to see you in April.

Regards,
Texas Society for Microscopy



--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 8 18:55:21 2005



From: Ken Converse :      kenconverse-at-qualityimages.biz
Date: Tue, 8 Mar 2005 20:04:58 -0500
Subject: [Microscopy] RE: SEM - LEO or JEOL?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,
I believe the countrates are correct. If you check
http://www.rontecusa.com/products.htm
I think you'll find they have a new detector design. It's lN2 free and
operates very fast. The resolution is not quite as good as their lN2 cooled
detectors, but the speeds are real.

I have no financial interest but did hear a talk on them in Woods Hole, MA
with NESM last May.

Ken Converse

owner 
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Monday, March 07, 2005 5:55 PM
To: Evren Cubukcu
Cc: MSA listserver


First of all, what method did you use to arrive
at the selection of an EVO SEM? Why not high vacuum?
I think that this needs to be factored if you're not sure.
What type of final specimen are you going to be analyzing?
If the soil is embedded, polished low mag imaged, you don't
necessarily need an ESEM. That said, if this is mostly all you
will be looking at, you can suffer the loss of resolution
of an ESEM. That you prefer not needing a chiller
is important. However, there are many advantages
in SEM systems that are only achieved by chiller-based
SEMs. You need to sort out all of the variables
for a SEM system first, I think, then select an
appropriate EDS system. In both cases, availability
of service resources is critical. If you don't have
a chiller, what cools the diffusion pump or turbo?
Some turbos can be air cooled.

If you are going to do an ESEM, it may likely
have a W filament or LaB6 at best. Thus, how
are you going to achieve 400K cps much less than
actually processing them? Furthermore, regardless
of the maximum cps stated, what filter time is this based on?
Short times can handle large cps at low resolution
and low fidelity. As filter time increases, dead time
increases and the existence of many counts becomes
irrellevant if not counterproductive. Most (my guess)
systems run about 2000 cps at 30% DT at 102uS time
constant. If you are concerned about resolution and
sensitivity, you should focus on obtaining long filter
time and { 35% DT. A system can certainly handle
hundreds of thousands cps but not likely at long filter
times. In this case, short filter times don't produce
good quant results. However, short times are valuable for
mapping. So, keep this in mind based on your application.
If you seek high resolution, you need a very good detector
and long filter times. Additionally, it seems out of
character to me that a W system would produce very high
counts compared to a LaB6 or especially an FESEM. But
I've only done EDS on FESEMs.

The other issue to keep in mind is that if you want to
do quant EDS, you will need an EDS package that allows
for high pressure quant. I use EDAX Genesis VIP Quant
for variable pressure SEM (usually 5-50Pa). It is selectable
for VP or high vacuum. Furthermore, it provides for
ZAF, PhiZAF and PhiRhoZAF quant in VP or HV modes.
The user interface is very nice, IMO. I have used the
Rontec as well and got good results in a high vacuum
system. I don't know if they can handle high pressure
situations.

There have been discussions about standardless quant
on this list before. The method I use is what EDAX calls
SEC--a standards correction factor. This is where the
quant value is typically value*SEC where SEC=1. However,
the SEC value can be changed to a value that makes the
quant result match a standard. Thus, quant results are
done routinely in a standardless manner but are traced
back to a standard. This SEC check should be done on
a routine basis (I do it every quarter). The other thing
to check is that the peak for each detected element lines
up with its respective eV for whichever shell is being
examined. This value can shift slightly. Sometimes,
an element's eV value might be high or low by a small
amount and confuse a convolution (peak pileup).

As far as the detector being LN2-free, I agree very much.
The tradeoffs for this option are cost and performance.
Without getting at or close to LN2 temperature, detector
performance will suffer. I use the EDAX Cryospec LN2-free
detector. This detector produces the same performance
as an LN2 detector. Currently, I am getting 131.5eV
resolution and can detect down to and including Be.
But of course, YMMV.

gary g.

At 12:44 AM 3/7/2005, you wrote:


} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America








From MicroscopyL-request-at-ns.microscopy.com Tue Mar 8 19:08:05 2005



From: Ellery Frahm :      frah0010-at-tc.umn.edu
Date: Tue, 8 Mar 2005 19:18:09 -0600
Subject: [Microscopy] Re: Spelling question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Based on the German words for "braking" (bremsen) and "radiation"
(strahlung), the spelling "bremsstrahlung" seems correct, as the
internet masses would seem to suggest. I also cannot find the spelling
"bremmstrahlung" in the books I have at hand. I would suggest sticking
with "bremsstrahlung" as the proper spelling. However, if any German
speakers care to differ with me, I'll happily stand corrected.

Best,
Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu



On Mar 8, 2005, at 4:27 PM, Garber, Charles A. wrote:

} Is there any opinion as to the preferred way of spelling:
}
} Bremmstrahlung (948 hits)
}
} or
}
} Bremsstrahlung (317,000 hits)
}
} radiation?
}
} If one does Google searches on each of the two spelllings, there is
} indeed
} indicated a preference of one over the other, as indicated above.
} Does that
} mean the second spelling is the correct one?
}
} Chuck
}
} ============================================
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 00:59:09 2005



From: randy-nessler-at-uiowa.edu (by way of MicroscopyListserver)
Date: Wed, 9 Mar 2005 01:09:12 -0600
Subject: [Microscopy] viaWWW: Insurance Service Provider Vs OEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (randy-nessler-at-uiowa.edu) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, March 8, 2005 at 15:32:26
---------------------------------------------------------------------------

Email: randy-nessler-at-uiowa.edu
Name: Randy Nessler

Organization: University of Iowa

Title-Subject: [Microscopy] [Filtered] MListserver:Insurance Service Provider Vs OEM

Question: Here we go again. I realize that this has been discussed
here before but we are faced with this decision again. I have been
lead to believe that a Big Ten Consortium has been formed between an
insurance provider and the Universities that are members of the Big
Ten. Our purchasing department has been involved in our discussions
with the insurance company (to the point of being a proponent?). I
would like to hear from any Big Ten University EM labs (or any
EM/confocal labs for that matter) that have evaluated this service
contract option recently. Anybody out there????
Thanks,
Randy Nessler
Phone 319-335-8142
randy-nessler-at-uiowa.edu

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 00:59:31 2005



From: mitrah-at-uci.edu (by way of MicroscopyListserver)
Date: Wed, 9 Mar 2005 01:09:39 -0600
Subject: [Microscopy] viaWWW: Myelin Fixation for EM Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mitrah-at-uci.edu) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, March 8, 2005 at 17:46:59
---------------------------------------------------------------------------

Email: mitrah-at-uci.edu
Name: Mitra Hooshmand

Organization: UC Irvine

Title-Subject: [Microscopy] [Filtered] MListserver: Myelin Fixation for EM Analysis

Question: I have been unsuccessfully attempting at obtaining optimal
myelin morphology for EM analysis. My ultimate goal is to do
immuno-EM on the spinal cord. However, I have not been able to get
myelin ultrastructure preservation even in doing EM alone.

If you have any recommendations/suggestions or a protocol that may
guide me, I would be most grateful. I am on an unforgiving deadline
and in dire need for some guidance. Thank you, immensely, in advance.

Mitra Hooshmand
Graduate Student Researcher
UC Irvine
mitrah-at-uci.edu

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 03:08:01 2005



From: Reinhard Rachel :      reinhard.rachel-at-biologie.uni-regensburg.de
Date: Wed, 09 Mar 2005 10:17:23 +0100
Subject: [Microscopy] Spelling question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Concerning the spelling and meaning of "Bremsstrahlung":

the comments given by Mike Bode and Bill Tivol are absolutely correct.

kind regards from Germany,

Reinhard

-------------------------------
PD Dr.Reinhard Rachel
Universität Regensburg
Lehrstuhl für Mikrobiologie
Universitaetsstr. 31
D-93053 Regensburg
tel.: +49 941 943 4534
fax.: +49 941 943 1824




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 03:44:57 2005



From: Gerd Leitinger :      gerd.leitinger-at-meduni-graz.at
Date: Wed, 09 Mar 2005 10:55:56 +0100
Subject: [Microscopy] Re: Re: Spelling question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Von: Ellery Frahm {frah0010-at-tc.umn.edu}
Betreff: Re: Spelling question
Datum: Tue, 8 Mar 2005 19:18:09 -0600
An: MICROSCOPY BB
{Microscopy-at-MSA.Microscopy.com}


"Bremsstrahlung" is the correct spelling.
As others have mentioned, it is derived from "bremsen (braking)" and
"Strahlung (radiation)".

The first s is pronounced like an "s", the second one like a "sh".
We have recently had a spelling reform wich changed the spelling of
several words, especially words containing sharp "ß"'s and double
"ss"`s, but to my knowledge the word "bremsen" has not been affected.

best wishes

Gerd

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Based on the German words for "braking" (bremsen) and "radiation"
} (strahlung), the spelling "bremsstrahlung" seems correct, as the
} internet masses would seem to suggest. I also cannot find the
spelling
} "bremmstrahlung" in the books I have at hand. I would suggest
sticking
} with "bremsstrahlung" as the proper spelling. However, if any
German
} speakers care to differ with me, I'll happily stand corrected.
}
} Best,
} Ellery
}
} --------------------
} Ellery E. Frahm
} Research Fellow & Manager
} Electron Microprobe Laboratory
} University of Minnesota - Twin Cities
} Department of Geology & Geophysics
} Lab Website: http://probelab.geo.umn.edu
}
}
}
} On Mar 8, 2005, at 4:27 PM, Garber, Charles A. wrote:
}
} } Is there any opinion as to the preferred way of spelling:
} }
} } Bremmstrahlung (948 hits)
} }
} } or
} }
} } Bremsstrahlung (317,000 hits)
} }
} } radiation?
} }
} } If one does Google searches on each of the two spelllings, there is
} } indeed
} } indicated a preference of one over the other, as indicated above.
} } Does that
} } mean the second spelling is the correct one?
} }
} } Chuck
} }
} } ============================================
} } Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} } President 1-800-2424-SPI
} } SPI SUPPLIES FAX: 1-610-436-5755
} } PO BOX 656 e-mail:cgarber-at-2spi.com
} } West Chester, PA 19381-0656 USA
}
}
}




Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 04:41:23 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Wed, 9 Mar 2005 07:20:21 -0330
Subject: [Microscopy] RE: RE: RE: SEM - LEO or JEOL?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ken Converse writes ...

} Gary,
} I believe the countrates are correct. If you check
} http://www.rontecusa.com/products.htm
} I think you'll find they have a new detector design.
} It's lN2 free and operates very fast. The resolution is
} not quite as good as their lN2 cooled detectors,
} but the speeds are real.

Our research group chose the Roentec for image analysis applications, but
the data for the 3rd generation Roentec XFlash SDD implies its energy
resolution is better than conventional Si(Li) beginning at input countrates
~80-100kcps. This comparison should be verified against the most recent
Si(Li) electronics announced this last summer. I'll be able to confirm and
provide more details after ours is installed.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



} -----Original Message-----
} } From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: Monday, March 07, 2005 5:55 PM
} To: Evren Cubukcu
} Cc: MSA listserver
} Subject: [Microscopy] RE: SEM - LEO or JEOL?
}
}
}
} ------------------------------------------------------------------
} ----------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------
} ----------
} ---
}
}
} First of all, what method did you use to arrive
} at the selection of an EVO SEM? Why not high vacuum?
} I think that this needs to be factored if you're not sure.
} What type of final specimen are you going to be analyzing?
} If the soil is embedded, polished low mag imaged, you don't
} necessarily need an ESEM. That said, if this is mostly all you
} will be looking at, you can suffer the loss of resolution
} of an ESEM. That you prefer not needing a chiller
} is important. However, there are many advantages
} in SEM systems that are only achieved by chiller-based
} SEMs. You need to sort out all of the variables
} for a SEM system first, I think, then select an
} appropriate EDS system. In both cases, availability
} of service resources is critical. If you don't have
} a chiller, what cools the diffusion pump or turbo?
} Some turbos can be air cooled.
}
} If you are going to do an ESEM, it may likely
} have a W filament or LaB6 at best. Thus, how
} are you going to achieve 400K cps much less than
} actually processing them? Furthermore, regardless
} of the maximum cps stated, what filter time is this based on?
} Short times can handle large cps at low resolution
} and low fidelity. As filter time increases, dead time
} increases and the existence of many counts becomes
} irrellevant if not counterproductive. Most (my guess)
} systems run about 2000 cps at 30% DT at 102uS time
} constant. If you are concerned about resolution and
} sensitivity, you should focus on obtaining long filter
} time and { 35% DT. A system can certainly handle
} hundreds of thousands cps but not likely at long filter
} times. In this case, short filter times don't produce
} good quant results. However, short times are valuable for
} mapping. So, keep this in mind based on your application.
} If you seek high resolution, you need a very good detector
} and long filter times. Additionally, it seems out of
} character to me that a W system would produce very high
} counts compared to a LaB6 or especially an FESEM. But
} I've only done EDS on FESEMs.
}
} The other issue to keep in mind is that if you want to
} do quant EDS, you will need an EDS package that allows
} for high pressure quant. I use EDAX Genesis VIP Quant
} for variable pressure SEM (usually 5-50Pa). It is selectable
} for VP or high vacuum. Furthermore, it provides for
} ZAF, PhiZAF and PhiRhoZAF quant in VP or HV modes.
} The user interface is very nice, IMO. I have used the
} Rontec as well and got good results in a high vacuum
} system. I don't know if they can handle high pressure
} situations.
}
} There have been discussions about standardless quant
} on this list before. The method I use is what EDAX calls
} SEC--a standards correction factor. This is where the
} quant value is typically value*SEC where SEC=1. However,
} the SEC value can be changed to a value that makes the
} quant result match a standard. Thus, quant results are
} done routinely in a standardless manner but are traced
} back to a standard. This SEC check should be done on
} a routine basis (I do it every quarter). The other thing
} to check is that the peak for each detected element lines
} up with its respective eV for whichever shell is being
} examined. This value can shift slightly. Sometimes,
} an element's eV value might be high or low by a small
} amount and confuse a convolution (peak pileup).
}
} As far as the detector being LN2-free, I agree very much.
} The tradeoffs for this option are cost and performance.
} Without getting at or close to LN2 temperature, detector
} performance will suffer. I use the EDAX Cryospec LN2-free
} detector. This detector produces the same performance
} as an LN2 detector. Currently, I am getting 131.5eV
} resolution and can detect down to and including Be.
} But of course, YMMV.
}
} gary g.
}
} At 12:44 AM 3/7/2005, you wrote:
}
}
} } -----------------------------------------------------------------
} ----------
} ---
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------
} ----------
} ----
} }
} } Thank you very much for such valuable comments and suggestions.
} } I have been in SEM field for 2 years now and your contributions
} are really
} } amazing.
} }
} } Evren
}
}
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 04:50:53 2005



From: Ken Converse :      kenconverse-at-qualityimages.biz
Date: Wed, 9 Mar 2005 06:00:17 -0500
Subject: [Microscopy] Re: SEM - LEO or JEOL?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,
Rontec also makes an lN2 series of detectors with resolution as good as 129
ev. Different people have different needs. Less resolution isn't
necessarily "junk" and high resolution without properly accounting for a lot
of variables will give you very precise "junk". Besides, if resolution is
so critical, why would you use EDS in the first place? You should be using
WDS on a probe with limited stage motion and light optics.

I have no financial interest. Just service the instruments these things get
bolted to.

Ken Converse

owner 
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Tuesday, March 08, 2005 10:26 PM
To: Ken Converse







From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 10:50:18 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 09 Mar 2005 11:56:16 -0800
Subject: [Microscopy] Re: viaWWW: Myelin Fixation for EM Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Find a paper, a review article or a book with EM's that you would
like to emulate. Then try their protocol. Without knowing something
about your experimental conditions, protocol and the problems you are
encountering it is difficult to make specific recommendations. Are you
fixing by immersion, perfusion, composition of buffer and fix,
post-fixation, dehydration, etc. etc? Also, keep in mind that optimal
preservation of immuno may not provide optimal perservation for morphology.

Geoff

by way of MicroscopyListserver wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (mitrah-at-uci.edu) from
} http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
} Tuesday, March 8, 2005 at 17:46:59
} ---------------------------------------------------------------------------
}
}
} Email: mitrah-at-uci.edu
} Name: Mitra Hooshmand
}
} Organization: UC Irvine
}
} Title-Subject: [Microscopy] [Filtered] MListserver: Myelin Fixation
} for EM Analysis
}
} Question: I have been unsuccessfully attempting at obtaining optimal
} myelin morphology for EM analysis. My ultimate goal is to do
} immuno-EM on the spinal cord. However, I have not been able to get
} myelin ultrastructure preservation even in doing EM alone.
}
} If you have any recommendations/suggestions or a protocol that may
} guide me, I would be most grateful. I am on an unforgiving deadline
} and in dire need for some guidance. Thank you, immensely, in advance.
}
} Mitra Hooshmand
} Graduate Student Researcher
} UC Irvine
} mitrah-at-uci.edu
}
} ---------------------------------------------------------------------------
}
}
}

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 13:17:04 2005



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Wed, 9 Mar 2005 19:26:42 +0000 (GMT)
Subject: [Microscopy] Literature on Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello listers,
While following the recent useful thread about costs
of running imaging facilities, I wondered what had
been written about setting up a microscopy imaging
facility.

The most recent reference that I can find is:
DeMaggio, Susan (2002) Running and Setting up a
Confocal Microscope Core Facility. Chapter 14 in:
Methods in Cell Biology 70: 475-485

I've trawled the archives of both listservers, and
maybe I've missed something. Perhaps this stuff isn't
written down. This particular reference seems to have
been written by a successful cytometrist.

Have any of you microscopists out there written
anything, or know of anything, that can be referred to
or cited?

Regards,
Jeremy

Send instant messages to your online friends http://uk.messenger.yahoo.com


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 14:19:03 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 10 Mar 2005 09:29:08 +1300
Subject: [Microscopy] Point Counter Supplier(s)?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Does anyone know who, these days, supplies microscope stage point counter
attachments for petrographic use?

We have an old one made by Swift, of England, but my Google searching has not
turned up any current suppliers or manufacturers.

tia

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 14:51:36 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 09 Mar 2005 16:00:35 -0500
Subject: [Microscopy] Re: Literature on Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jeremy,
The only comprehensive source I know of is 30 years old...Its Volume
4 in the "Practical Methods in Electron Microscopy" series edited by
Audrey Glauert: "Design of the Electron Microscope Laboratory" by
Ronald H. Alderson (1975) North-Holland.
I am in the process of planning a relocation of my facility, and this
book is still extremely helpful.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 15:40:57 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 09 Mar 2005 16:49:48 -0500
Subject: [Microscopy] Re: Literature on Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jeremy,
Requirements for imaging facilities vary depending on the type of equipment
it will contain. Also you are often constrained by the location of the
facility.

For example, vibration problems for LM can often be overcome by using
weighted tables. High resolution SEM or TEM often requires very stable
sub-basements or intricate vibration mounts.

One presentation, and related discussion, at the Core Facility Management
session at M&M 2005 will be on setting up an appropriate environment for
high-resolution TEM taking into consideration vibration, electric fields,
temperature shifts, and acoustic problems.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 3/9/05 2:26 PM, "Jeremy Sanderson" {jb_sanderson-at-yahoo.com} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Hello listers,
} While following the recent useful thread about costs
} of running imaging facilities, I wondered what had
} been written about setting up a microscopy imaging
} facility.
}
} The most recent reference that I can find is:
} DeMaggio, Susan (2002) Running and Setting up a
} Confocal Microscope Core Facility. Chapter 14 in:
} Methods in Cell Biology 70: 475-485
}
} I've trawled the archives of both listservers, and
} maybe I've missed something. Perhaps this stuff isn't
} written down. This particular reference seems to have
} been written by a successful cytometrist.
}
} Have any of you microscopists out there written
} anything, or know of anything, that can be referred to
} or cited?
}
} Regards,
} Jeremy
}
} Send instant messages to your online friends http://uk.messenger.yahoo.com
}




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 9 19:13:23 2005



From: moss-at-relia.net (by way of MicroscopyListserver)
Date: Wed, 9 Mar 2005 19:23:29 -0600
Subject: [Microscopy] viaWWW: Zeiss 902 manuals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (moss-at-relia.net) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, March 9, 2005 at 13:20:41
---------------------------------------------------------------------------

Email: moss-at-relia.net
Name: William McManus

Organization: Mt Ogden Scientific Services

Title-Subject: [Microscopy] [Filtered] Zeiss 902 manuals

Question:
I am working on a Zeiss 902, and need to find a set of tech and
service manuals in english. If anyone has access to any please
contact me.
Bill

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 01:57:31 2005



From: Gareth Morgan :      Gareth.Morgan-at-labmed.ki.se
Date: Thu, 10 Mar 2005 09:07:39 +0100
Subject: [Microscopy] Re: Literature on Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I would also be interested in other peoples experience in this. I run a
LASER dissection plus an image analysis core facility here and for me it is
just a matter of trial and error (maybe mostly error!?) as a
'sideline/spare time' activity alongside other (significant) teaching
commitments.

See
http://labmed.ki.se/avdelningar/patol/core_facility/corefacility
This is not an advertisement - I cannot imagine that any of you would want
to use us.

All the best

Gareth


With best wishes,

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Histo/cytopathology, Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 09:38:38 2005



From: Fred Pearson :      eoptics-at-mcmaster.ca
Date: Thu, 10 Mar 2005 10:49:57 -0500 (Eastern Standard Time)
Subject: [Microscopy] Announcement of Microscopical Society of Canada (MSC) Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To the Microscopy Community:

I would like to draw your attention to the microscopy meeting of the
MSC to be held at McMaster University in Hamilton Ontario Canada in May
2005. The conference will held on May 18-20. Workshops will be on
May 16-17.

We have prepared a superb program covering themes such as:

synchrotron-base microscopy,
electron microscopy (TEM and low voltage/environmental SEM),
tomography of biological and materials structures,
scanning probe techniques,
spectroscopy in the TEM,
focused ion beam microscopy of bio/materials etc.
advances in instrumentation

Prior to the meeting, there will be a series of worskhops jointly held
with the 4th Annual Brockhouse Institute for Materials Research meeting.
The Workshops will include:

cryomicroscopy,
confocal and fluorescence microscopy,
image analysis,
scanning probe methods
EBSD
spectromicroscopy
microtomy
energy loss spectroscopy and energy filtered imaging
high-resolution electron microscopy (quantitative measurement of
strains at defects)
and many more.

Please see
http://www.brockhouse.mcmaster.ca/MSC-SMC2005/
for a full program, speakers, registrations etc.

There will be exhibits from manufacturers in microscopy-related tools
from May 18 to the 20. This is the perfect occasion to hear about
the latest developments and see demonstrations.

There will demos on:
FIB ( with environmental SEM), FE-SEM, EBSD.

The Science Advisor to the Prime Minister of Canada, Art Carty, will be at
the welcoming mixer on Tuesday (joint event with the Brockhouse Institute
Annual workshop mixer).

Please pass this on to your research group, post the announcement in
your laboratories and encourage your group to attend. This is going to
be a really excellent meeting and you should not miss this opportunity
to participate to this event.

Note the Abstract submission deadline 15th March, Registration deadline
15 April. Register early to keep a space in the workshops.
Kind regards to all of you. Hope to see you in May.


Gianluigi Botton, (on behalf of the organising committee)




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 10:28:17 2005



From: Aleksandr Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Thu, 10 Mar 2005 17:37:17 +0000
Subject: [Microscopy] Soda lime glass coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,
I'm forwarding this email that I received in the hope that our vast pool of
knowledge, info and contacts might be put to use again. I would have
started this person out with Peter Stoltzenberg, but as many of you realize,
he is sadly out of the service business. Is there anyone else who is
familiar with the Siemens 1A, preferably in the South? Please respond
directly to Onesimus Otieno at ootieno-at-oaks1.oakwood.edu . Thanks in
advance.

Ken Converse

owner 
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
} From: ootieno-at-oaks1.oakwood.edu [mailto:ootieno-at-oaks1.oakwood.edu]
Sent: Wednesday, March 09, 2005 2:47 PM
To: kenconverse-at-qualityimages.biz

Dear listers,

I desperately need soda lime glass coverslips. I've tried to search the
suppliers on the Interent but without any success. I cannot use the
usual borosilicate coverslips as they seemingly inhibit the gold
enhancement reaction. I cannot use soda lime glass slides because they
are too thick for my purposes.
I will greatly appreciate any advice.

--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 11:36:31 2005



From: K.N. Bozhilov :      bozhilov-at-ucr.edu
Date: Thu, 10 Mar 2005 09:46:26 -0800
Subject: [Microscopy] Defocus spread

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am wondering whether there is alternative to determining the defocus
spread on a HR TEM without an energy filtering capabilities in addition
to the Young's fringe method of Frank and the elaborate measurements of
actual voltage, beam current, and lens current fluctuations.

_____________________________________________
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel 951 827 2998
fax 951 827 2489
________________________________________________



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 15:07:41 2005



From: Hong Yi :      hyi-at-emory.edu
Date: Thu, 10 Mar 2005 16:17:41 -0500
Subject: [Microscopy] (Microscopy)Re: CCD camera on TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All:

           We had a Gatan BioScan CCD camera installed on our TEM about
6 years ago when we bought the scope, but for some reason we have not
used the camera much. Now our situation has changed that we will
benefit from using a CCD camera so we want to get it running
again. However, we need to switch our computer from a Mac to a PC and
upgrade the software for the camera. I got a price quotation from the
company already. But my concern is that the model has been around for
so long and technology probably has improved a lot since, so is it
worth it for us to upgrade only software? I would like to hear opinions
from people who have experience with Gatan BioScan and other CCD. We
are a multi-user facility, so the samples we look at on the scope can
be anything from nano magnetic particles to rat brain. 

           Thank you in advance. 

Hong
Emory EM



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 16:47:08 2005



From: Gordon Couger :      gcc-at-couger.com
Date: Thu, 10 Mar 2005 16:56:41 -0600
Subject: [Microscopy] Re: Soda lime glass coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Will plastic cover slips work?

Do your have to equipment to make thin sections for geology
While they would be rather expensive you should be able to make
soda lime cover slips from soda lime slides by grinding them
down and polishing them.

If you have sputtering equipment you might be able to sputter a
coating on regular cover slips that does not inhibit your reaction.

Good luck
Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org

Aleksandr Mironov wrote:
}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Dear listers,
}
} I desperately need soda lime glass coverslips. I've tried to search the
} suppliers on the Interent but without any success. I cannot use the
} usual borosilicate coverslips as they seemingly inhibit the gold
} enhancement reaction. I cannot use soda lime glass slides because they
} are too thick for my purposes.
} I will greatly appreciate any advice.
}



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 16:51:27 2005



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 10 Mar 2005 18:01:04 -0500
Subject: [Microscopy] Soda lime glass coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't think that I am aware of any soda lime coverslips. If you find them, I would be interested in them for purposes of my own.

I don't know what " the gold enhancement reaction" is, but I do have a couple of suggestions.

1) You might consider dipping your borosilicate coverslips slowly into a diluted sodium silicate solution (also known as water glass) and then heating them up afterward ( I would suggest 500 -600C). This might put enough Na into the glass to give you similar properties as what you want. You might even be able to use the water glass directly dipping it onto other substrates or a glass slide.

2) You can use some of the standard mechanical thinning processes to thin float glass to about 100 um thick. Talk to any of the TEM prep guys for the physical sciences, e.g South Bay Technology, Gatan, E. A Fischione, and several others. There are devices that will allow you to make parallel sided samples to either the Sn side or air side of the glass. (In the dark, the Sn side will fluoresce with UVB illumination.) By using standard polishing techniques, you can get your polished side to be very smooth without much difficulty. Talk to some people in the physical sciences there that are doing TEM. I'm sure that they can help you out.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)


-----Original Message-----
} From: Aleksandr Mironov [mailto:Aleksandr.Mironov-at-manchester.ac.uk]
Sent: Thursday, March 10, 2005 12:37 PM
To: Microscopy-at-microscopy.com

Dear listers,

I desperately need soda lime glass coverslips. I've tried to search the
suppliers on the Interent but without any success. I cannot use the
usual borosilicate coverslips as they seemingly inhibit the gold
enhancement reaction. I cannot use soda lime glass slides because they
are too thick for my purposes.
I will greatly appreciate any advice.

--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk






From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 17:00:14 2005



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Thu, 10 Mar 2005 15:09:34 -0800
Subject: [Microscopy] (Microscopy)Re: CCD camera on TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Hong,
We have a Gatan's 1x1K CCD about the same age as yours that is being used heavily (on a daily basis). We are still running the old Mac-based Digital Micrograph for image acquisition, but we do most of the image analyses on a newer PC DM version. Likewise, we image both biological and material science samples, and the camera has proven itself to be a great workhorse piece of equipment.

Best regards,
Alice.


Alice Dohnalkova
Environmental Microbiology
Pacific Northwest National Laboratory
Richland, WA 99352
(509) 372-0692 office
(509) 376-3654 TEM lab

-----Original Message-----
} From: Hong Yi [mailto:hyi-at-emory.edu]
Sent: Thursday, March 10, 2005 1:18 PM
To: Microscopy-at-microscopy.com

Dear All:

           We had a Gatan BioScan CCD camera installed on our TEM about
6 years ago when we bought the scope, but for some reason we have not
used the camera much. Now our situation has changed that we will
benefit from using a CCD camera so we want to get it running
again. However, we need to switch our computer from a Mac to a PC and
upgrade the software for the camera. I got a price quotation from the
company already. But my concern is that the model has been around for
so long and technology probably has improved a lot since, so is it
worth it for us to upgrade only software? I would like to hear opinions
from people who have experience with Gatan BioScan and other CCD. We
are a multi-user facility, so the samples we look at on the scope can
be anything from nano magnetic particles to rat brain. 

           Thank you in advance. 

Hong
Emory EM






From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 18:33:16 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 10 Mar 2005 16:46:33 -0800
Subject: [Microscopy] Re: (Microscopy)Re: CCD camera on TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Hong
We have Gatan's 600W BioScan camera for about 3 years and very happy. This
is very good camera for most applications. Because it's top-mount camera,
you could not expect "atomic" resolution on this camera. From another hand
you have bigger area of view, which sometime is very useful especially in
biological applications. For really fine works (material science mostly)
you need bottom-mount camera like UltraScan. Basically, (if you have
enough money and want "go digital") you need to have both: bottom and top
mounted cameras. Both would be operated from the same software
(DigitalMicrograph), which is quite convenient. I do find
DigitalMicrograph (DM) software quite good and easy to manipulate. I don't
like the way, how you export images into the TIFF with DM - a lot of mouse
clicks and I was not able to create workable macros to automate the job
(16-bit images). You also lost scale bar... Finally I gave up and export
only for publications (which I believe was intention of DM
creators). Anyway, I am quite happy user and have no interest in Gatan
unless I'll find money for UltraScan.
Sergey

At 04:17 PM 3/10/2005 -0500, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 20:01:55 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 11 Mar 2005 15:12:05 +1300
Subject: [Microscopy] Point Counters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

In case anyone else needs to know, I am told that Swift doesn't make point counters
any longer, but that Leica does.


cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 10 21:58:28 2005



From: Gazda, Jerzy :      jerzy.gazda-at-ceriumlabs.com
Date: Thu, 10 Mar 2005 22:07:49 -0600
Subject: [Microscopy] (Microscopy)Re: CCD camera on TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hong,
We have two microscopes equipped with Gatan MSC794 CCD cameras, one of them had a PC interface for the last six years, the second one came originally on MAC running DM2.5. Two years ago we switched to IEEE1394 Firewire control and modern PC operations on both of these cameras. The hardware cost and software cost was in my opinion minimal as compared to purchases of brand new cameras and that was not even considered.

Having said that, going form Mac to PC had required learning new habits and dealing with few bugs in scripts and DM software. Overall the transition was painless and benefits of having modern computers and an upgrade path for both hardware and software, priceless. We do not use film routinely and on one of the microscopes never had.

Regards,

Jerzy
******************************************************
Jerzy Gazda, Ph.D. CeriumLaboratories L.L.C.
Supervising Engineer 5204 E. Ben White Blvd. - MS 512
Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453
jerzy.gazda-at-ceriumlabs.com
******************************************************




-----Original Message-----
} From: Hong Yi [mailto:hyi-at-emory.edu]
Sent: Thursday, March 10, 2005 3:18 PM
To: Microscopy-at-microscopy.com

Dear All:

           We had a Gatan BioScan CCD camera installed on our TEM about
6 years ago when we bought the scope, but for some reason we have not used the camera much. Now our situation has changed that we will benefit from using a CCD camera so we want to get it running again. However, we need to switch our computer from a Mac to a PC and upgrade the software for the camera. I got a price quotation from the company already. But my concern is that the model has been around for so long and technology probably has improved a lot since, so is it worth it for us to upgrade only software? I would like to hear opinions from people who have experience with Gatan BioScan and other CCD. We are a multi-user facility, so the samples we look at on the scope can be anything from nano magnetic particles to rat brain. 

           Thank you in advance. 

Hong
Emory EM







From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 00:46:15 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Thu, 10 Mar 2005 22:55:36 -0800
Subject: [Microscopy] Re: (Microscopy)Re: CCD camera on TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sergey;
Gatan has a plug-in that will convert images to any format you
want. Two mouse clicks will convert as many images as you can fit in a
folder. Sound good? You will love it. Also, DM 3.9 has the converter
built-in.

John Mardinly
Intel

-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Thursday, March 10, 2005 4:47 PM
To: Microscopy-at-microscopy.com

Dear Hong
We have Gatan's 600W BioScan camera for about 3 years and very happy.
This
is very good camera for most applications. Because it's top-mount
camera,
you could not expect "atomic" resolution on this camera. From another
hand
you have bigger area of view, which sometime is very useful especially
in
biological applications. For really fine works (material science mostly)

you need bottom-mount camera like UltraScan. Basically, (if you have
enough money and want "go digital") you need to have both: bottom and
top
mounted cameras. Both would be operated from the same software
(DigitalMicrograph), which is quite convenient. I do find
DigitalMicrograph (DM) software quite good and easy to manipulate. I
don't
like the way, how you export images into the TIFF with DM - a lot of
mouse
clicks and I was not able to create workable macros to automate the job
(16-bit images). You also lost scale bar... Finally I gave up and export

only for publications (which I believe was intention of DM
creators). Anyway, I am quite happy user and have no interest in Gatan
unless I'll find money for UltraScan.
Sergey

At 04:17 PM 3/10/2005 -0500, you wrote:


} -----------------------------------------------------------------------
-------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} the camera. I got a price quotation from the company already. But my
} concern is that the model has been around for so long and technology
} probably has improved a lot since, so is it worth it for us to upgrade

} only software? I would like to hear opinions from people who have
} experience with Gatan BioScan and other CCD. We are a multi-user
} facility, so the samples we look at on the scope can be anything from
} nano magnetic particles to rat brain.
}
} Thank you in advance.
}
} Hong
} Emory EM
}





From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 01:25:43 2005



From: akoorts-at-medic.up.ac.za (by way of Ask-A-Microscopist)
Date: Fri, 11 Mar 2005 01:35:51 -0600
Subject: [Microscopy] AskAMicroscopist: Prussian Blue iron stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (akoorts-at-medic.up.ac.za) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Friday, March 11, 2005 at 00:03:53
---------------------------------------------------------------------------

Email: akoorts-at-medic.up.ac.za
Name: Alida Koorts

Organization: University of Pretoria

Education: Graduate College

Location: Pretoria, South Africa

Question: Is it possible to perform a Prussian Blue iron stain on LR
White sections for light microscopy. If so I would very much
appreciate a detailed protocol.
Best regards
Alida

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 07:16:12 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Fri, 11 Mar 2005 09:56:19 -0330
Subject: [Microscopy] printing round labels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I hope this doesn't deviate too far from the scope of this list ... but I am
having problems finding what appears to be a unique label printer. As part
of a high-throughput sample preparation workflow, we need to print
individual 1" round labels. What methods are others using?

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
{www.micro-investigations.com}



From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 09:10:47 2005



From: Ellery Frahm :      frah0010-at-tc.umn.edu
Date: Fri, 11 Mar 2005 09:49:58 -0600
Subject: [Microscopy] magnetic lenses; classroom example

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Michael
Avery produce round labels for laser and inkjet printing, and the software
for printing them using various wordprocessors
using standard printers
see e.g.

http://www.avery.com/us/Main?action=product.HierarchyList&node=10211270&catalogcode=WEB01

Chris

----- Original Message -----
} From: "michael shaffer" {michael-at-shaffer.net}
To: "Microscopy list" {Microscopy-at-MSA.Microscopy.Com}
Sent: Friday, March 11, 2005 1:26 PM

Microscopy folks,

I am always looking for interesting visual aids for the course I teach
on scanning electron microscopy and X-ray microanalysis. For instance,
I have an old WD spectrometer that I bring to lecture, let the student
turn the screw that moves the dispersing crystal and detector, etc.

For lectures on magnetic lenses, I'd like to use either unwound
windings from an old magnetic lens or just an equivalent length and
gauge of copper wire. Unfortunately, it is difficult to find online
suppliers of old or dead electron microscopy components (not much
demand, I suspect), and I cannot find any references (or even rough
estimates) to the number of windings in, say, a typical condenser lens
or what the gauge of that wire would be.

Any help with either of these endeavors would be much appreciated.

Thanks,
Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu



From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 10:56:41 2005



From: Michael O'Keefe :      MAOK-at-lbl.gov
Date: Fri, 11 Mar 2005 09:06:26 -0800
Subject: [Microscopy] [Fwd: Defocus spread]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Michael O'Keefe wrote:

} Krassimir,
}
} You mention two methods of determining defocus spread. Like you, I'd also be
} interested to know of any third way.
}
} 1. Defocus spread is a function of beam energy spread plus variations
} (instabilities) in lens current and high voltage times the coefficient of chromatic
} aberration -- so you can measure all these and compute the defocus spread.
} Problems include getting access to the lens current and measuring fluctuations of
} the order of one part per million, measuring beam energy spread (with a filter you
} can measure the energy spread from the tip with the combined HT instabilities by
} integrating the zero-loss peak over a typical image collection time -- this is
} safer than trying to get access to the HT (preferably at the gun) and measuring
} parts per million at several hundred kV).
}
} 2. Microscope information limit (where the Young's fringes die out) depends on
} defocus spread -- so you can get an experimental measurement (estimate?) of the
} defocus spread by measuring the extent of the fringes. Problems include the
} specimen (very thin, amorphous, good scatterer) and exact magnification
} calibration. Also, Young's fringes from a thicker specimen can theoretically
} extend beyond the information limit.
}
} Mike
}
} ------------------------
} Dr. Michael A. O’Keefe
} Director, Microscopy Society of America
} Lawrence Berkeley National Laboratory
} Materials Sciences Division
} 1 Cyclotron Road
} Berkeley, CA 94720
} 510-486-4610
} 510-486-5530 fax
} maok-at-lbl.gov
}
} "K.N. Bozhilov" wrote:
}
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} } I am wondering whether there is alternative to determining the defocus
} } spread on a HR TEM without an energy filtering capabilities in addition
} } to the Young's fringe method of Frank and the elaborate measurements of
} } actual voltage, beam current, and lens current fluctuations.
} }
} } _____________________________________________
} } Krassimir N. Bozhilov
} } Central Facility for Advanced Microscopy and Microanalysis
} } University of California
} } Riverside, CA 92521
} }
} } tel 951 827 2998
} } fax 951 827 2489
} } ________________________________________________



From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 12:43:25 2005



From: pgrover :      pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 11 Mar 2005 13:53:38 -0500
Subject: [Microscopy] re: magnetic lenses; classroom example

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The EM center in agriculture at Purdue had a very nicely made bisected
column showing the condensor lens windings in cross section, truly inspiring
to look at. I remember counting wires in a given area under a stereoscope
and calculating the length of the coil. Debby Sherman probably could tell
you more. I have a couple of old columns I've salvaged and would someday
like to do likewise, but it probably would not be easy, requiring a good
metal cutting bandsaw, then potting the exposed lens in epoxy, then surface
polishing etc. But I guess it'd be about as easy to make two or four
hemi-columns as it would be to make one. I'd be happy to send one of them
to a good home.

Paul Grover
-------------------------------------------------------------------------
"'Why listen, Lady,' he said with a grin of delight 'the monks of old
slept in their coffins!' 'They wasn't as advanced as we are.' the old
woman said." - The Life You Save May be Your Own


----------------------------------------------------------------------------
--
The Microscopy ListServer -- Sponsor: The Microscopy Society of America To
Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver

Microscopy folks,

I am always looking for interesting visual aids for the course I teach
on scanning electron microscopy and X-ray microanalysis. For instance,
I have an old WD spectrometer that I bring to lecture, let the student
turn the screw that moves the dispersing crystal and detector, etc.

For lectures on magnetic lenses, I'd like to use either unwound
windings from an old magnetic lens or just an equivalent length and
gauge of copper wire. Unfortunately, it is difficult to find online
suppliers of old or dead electron microscopy components (not much
demand, I suspect), and I cannot find any references (or even rough
estimates) to the number of windings in, say, a typical condenser lens
or what the gauge of that wire would be.

Any help with either of these endeavors would be much appreciated.

Thanks,
Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu





From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 12:51:04 2005



From: Quinn, Tim L. :      quinntl-at-umkc.edu
Date: Fri, 11 Mar 2005 12:57:39 -0600
Subject: [Microscopy] mouse serum blocking solution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi gang!

I need to make up a batch of mouse serum blocking buffer to block free biotinylation used on biotintylated mouse Ab on rat tissue.

Does anyone have a recipe mouse serum blocking buffer?

Cheers,

Timothy L. Quinn
Senior Lab Technician
UMKC Medical School
Med Lab
Laboratory of Pulmonary &
Infectious Disease
M3-202
2411 Holmes St
Kansas City, MO 64108-2792

Phone: 816-235-1904
Fax: 816-235-5194
E-mail: quinntl-at-umkc.edu



From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 14:28:14 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 11 Mar 2005 15:40:32 -0800
Subject: [Microscopy] Re: AskAMicroscopist: Prussian Blue iron stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Alida:

Since immunostaining reagents can penetrate LR White, I would think that
the reagents for Prussian blue would as well. This is a very simple
"stain" (a reaction really), any histotechnique text or website will
have it. It is VERY important the the HCl-potassium ferrocyanide
solution be mixed immediately before use.

Geoff

by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (akoorts-at-medic.up.ac.za) from
} http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
} on Friday, March 11, 2005 at 00:03:53
} ---------------------------------------------------------------------------
}
}
} Email: akoorts-at-medic.up.ac.za
} Name: Alida Koorts
}
} Organization: University of Pretoria
}
} Education: Graduate College
}
} Location: Pretoria, South Africa
}
} Question: Is it possible to perform a Prussian Blue iron stain on LR
} White sections for light microscopy. If so I would very much
} appreciate a detailed protocol.
} Best regards
} Alida
}
} ---------------------------------------------------------------------------
}
}
}

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 18:35:52 2005



From: Jan Leunissen :      leunissen-at-aurion.nl
Date: Sat, 12 Mar 2005 13:45:15 +1200
Subject: [Microscopy] Immunoreagents penetrating into LR White?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kia Ora (albeit with a Dutch accent),

Has anyone observed that immunoreagents actually penetrate into
LR-white sections? It seems so unlikely to me. Antibodies being 8-12nm
in size, not even taking into account the size increase caused by any
marker attached....... Wouldn't an ultrathin section, that is anything
between let's say 40 and 70 nm thick, look like a slice of Gouda Cheese
instead of a smooth and even layer? Depending on fixation and maybe
other treatments penetration into even fully hydrated ultrathin
cryosections is usually limited to antibodies and the very small gold
particle immunoconjugates, larger ones not getting deeper than the
surface. York-Dieter Stierhof from Tübingen, Germany, did some
beautiful work on this topic.
I am really interested in this and would appreciate to hear of anyone's
experience.

BTW, I have no commercial interest in Gouda Cheese...

Jan Leunissen
Aurion Present Address:
Costerweg 5 EM-Unit
6702 A Wageningen Otago School of Medicine
The Netherlands Dunedin, New Zealand
phone 31-317-497676 phone 64-3-4797109
fax 31-317-415955 fax 64-3-4797254
http://www.aurion.nl http://ocem.otago.ac.nz
------------------------------------------------------------------------
--------
"Light Microscopy? That is for people on a diet ". . . Eva Leunissen
(12)


On Mar 12, 2005, at 11:40 am, Geoff McAuliffe wrote:

} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Greetings Alida:
}
} Since immunostaining reagents can penetrate LR White, I would think
} that the reagents for Prussian blue would as well. This is a very
} simple "stain" (a reaction really), any histotechnique text or website
} will have it. It is VERY important the the HCl-potassium ferrocyanide
} solution be mixed immediately before use.
}
} Geoff
}
} by way of Ask-A-Microscopist wrote:
}
} } ----------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ---------
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } submitted by (akoorts-at-medic.up.ac.za) from
} } http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-
} } Microscopist.html on Friday, March 11, 2005 at 00:03:53
} } ----------------------------------------------------------------------
} } -----
} }
} } Email: akoorts-at-medic.up.ac.za
} } Name: Alida Koorts
} }
} } Organization: University of Pretoria
} }
} } Education: Graduate College
} }
} } Location: Pretoria, South Africa
} }
} } Question: Is it possible to perform a Prussian Blue iron stain on LR
} } White sections for light microscopy. If so I would very much
} } appreciate a detailed protocol.
} } Best regards
} } Alida
} }
} } ----------------------------------------------------------------------
} } -----
} }
} }
}
} --
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu
} **********************************************
}
}
}
}
}
}




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 22:51:32 2005



From: kellymariegreen-at-hotmail.com (by way of Ask-A-Microscopist)
Date: Fri, 11 Mar 2005 23:01:43 -0600
Subject: [Microscopy] AskAMicroscopist: lectures on electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (kellymariegreen-at-hotmail.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Friday, March 11, 2005 at 08:27:35
---------------------------------------------------------------------------

Email: kellymariegreen-at-hotmail.com
Name: Kelly Green

Organization: UWE

Education: Graduate College

Location: Bristol, UK

Question: After several lectures on electron microscopy (including
sem, tem and edx) i am still very confused. Are there any good
reviews to start me off with the methods and biomedical diagnostic
applications?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 11 22:53:13 2005



From: echeung-at-eyetech.com (by way of MicroscopyListserver)
Date: Fri, 11 Mar 2005 23:03:03 -0600
Subject: [Microscopy] viaWWW: removing glass coverslips from cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (echeung-at-eyetech.com) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, March 11, 2005 at 17:29:24
---------------------------------------------------------------------------

Email: echeung-at-eyetech.com
Name: Eunice Cheung

Organization: eyetech

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi.
I was wondering if there were any available step by step protocols on
removing glass coverslips from cells grown on them. I have been
trying to study the en face structure of cells. 1) growing them on
coverslips 2)Processing them with Spurrs for ultramicronomy 3) using
BEEM capsules filled with Spurrs resin and inverting them in cotact
with the glass coverslips 4) After polymerizing, using heat to melt
and remove the coverslip. 5) Section and view them on TEM. So far,
the surface of the cells have been stuck to the coverslip after
removal or glass pieces stuck to the sample.
I am open to suggestions for any other strategy.
Thanks.
Eunice


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Mar 12 08:30:23 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Sat, 12 Mar 2005 10:44:00 -0600
Subject: [Microscopy] Fwd: viaWWW: removing glass coverslips from cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You might try hydrofluoric acid. I have used it to remove coverslips
from cell cultures embedded in epoxy resin. As always, use extreme
caution when using acids/bases.
Randy

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:echeung-at-eyetech.com]
Sent: Friday, March 11, 2005 11:03 PM
To: microscopy-at-ns.microscopy.com

I do this all the time with an epoxy (EmBed812) resin. after a short
polymerization (about 7-8 hrs) at 60 C, I slowly immerse the coverslip into
liquid nitrogen and the resin pops off. if i polymerize longer, the bond
between glass and plastic is sometimes too strong and the glass fractures
such that it sticks with the block and prevents sectioning. After
detaching the coverslip, I return the block to the oven for further
polymerization. I recommend you stay away from HF; it is nasty stuff and
should not be needed in this application. good luck.



} ----------
}
} Email: echeung-at-eyetech.com
} Name: Eunice Cheung
}
} Organization: eyetech
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: Hi.
} I was wondering if there were any available step by step protocols on
} removing glass coverslips from cells grown on them. I have been trying to
} study the en face structure of cells. 1) growing them on coverslips
} 2)Processing them with Spurrs for ultramicronomy 3) using BEEM capsules
} filled with Spurrs resin and inverting them in cotact with the glass
} coverslips 4) After polymerizing, using heat to melt and remove the
} coverslip. 5) Section and view them on TEM. So far, the surface of the
} cells have been stuck to the coverslip after removal or glass pieces stuck
} to the sample.
} I am open to suggestions for any other strategy.
} Thanks.
} Eunice
}
}
} ---------------------------------------------------------------------------

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Sat Mar 12 19:32:23 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Sat, 12 Mar 2005 17:41:32 -0800
Subject: [Microscopy] Re: Literature on Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If you are looking for articles on designing/setting up microscopy
facilities, I wrote one a couple of years ago for Microscopy Today.
Previous to that one of my articles also appears in Protocols for
Electron Microscopy by John Wiley.

If you cannot find it in the archives for Microscopy Today, let me know.

Murphy, Judy A., 2002. Designing A Microscopy/Analytical
Instrumentation Facility: Step By Step Procedure, Microscopy Today,
Nov., 2002.

Murphy, Judy A., 1993. Designing A Microscopy Facility: Step by Step
Procedure. Procedures in Electron Microscopy 7:5.1-7:5.31, John Wiley
and Sons, New York.


along the same thread
Murphy, Judy A., 2001. Image Management For A Multi-Instrument,
Multi-Platform Microscopy Facility, Scanning, May,2001.

There are several others I wrote, but these should get you started.

Cheers,
Judy



Judy Murphy, PhD
Microscopy, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net







Jeremy Sanderson wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Sun Mar 13 10:54:16 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Sun, 13 Mar 2005 09:03:38 -0800 (PST)
Subject: [Microscopy] Re: viaWWW: removing glass coverslips from cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

well you pretty much have it figured out. the only
suggestion i have is to use araldite(sp?). it is
slightly softer and the coverslips are more easily
removed.
good luck
john
--- by way of MicroscopyListserver
{echeung-at-eyetech.com} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Below is the result of your feedback form
} (NJZFM-ultra-55). It was
} submitted by (echeung-at-eyetech.com) from
}
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html
} on
} Friday, March 11, 2005 at 17:29:24
}
---------------------------------------------------------------------------
}
} Email: echeung-at-eyetech.com
} Name: Eunice Cheung
}
} Organization: eyetech
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: Hi.
} I was wondering if there were any available step by
} step protocols on
} removing glass coverslips from cells grown on them.
} I have been
} trying to study the en face structure of cells. 1)
} growing them on
} coverslips 2)Processing them with Spurrs for
} ultramicronomy 3) using
} BEEM capsules filled with Spurrs resin and inverting
} them in cotact
} with the glass coverslips 4) After polymerizing,
} using heat to melt
} and remove the coverslip. 5) Section and view them
} on TEM. So far,
} the surface of the cells have been stuck to the
} coverslip after
} removal or glass pieces stuck to the sample.
} I am open to suggestions for any other strategy.
} Thanks.
} Eunice
}
}
}
---------------------------------------------------------------------------
}
}



__________________________________
Do you Yahoo!?
Yahoo! Small Business - Try our new resources site!
http://smallbusiness.yahoo.com/resources/


From MicroscopyL-request-at-ns.microscopy.com Sun Mar 13 14:26:06 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 14 Mar 2005 09:36:15 +1300
Subject: [Microscopy] more on point counters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

in the interests of completeness, and in case there are others interested........

My attention has been drawn also to www.petrog.com, a site which advertises not only a
point-counting stage, but a digital image-analysing suite for optical-microscopical
petrography.

While I appreciate the replies I have had on this subject off-list, I do wish that replies to
postings in general would be made on-list, so that everyone can read the answers as
well as the questions.

I know that advertising is banned, but when a lister asks if a such-and-such is available,
surely it is reasonable enough, and useful, for a supplier to reply ON-LIST to say that
the such-and-such is available from them, isn't it?

cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 08:40:53 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Mon, 14 Mar 2005 09:51:22 -0500
Subject: [Microscopy] viaWWW: removing glass coverslips from cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have always used liquid nitrogen to remove the cover glass. I drop the whole
capsule (with cover glass) into liquid nitrgon, wait a few seconds, remove (with
tongs) and the cover glass ususally just pops off, the cells remain on the
capsule.



Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: echeung-at-eyetech.com [mailto:echeung-at-eyetech.com]
Sent: Saturday, March 12, 2005 12:03 AM
To: microscopy-at-ns.microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (echeung-at-eyetech.com) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, March 11, 2005 at 17:29:24
---------------------------------------------------------------------------

Email: echeung-at-eyetech.com
Name: Eunice Cheung

Organization: eyetech

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi.
I was wondering if there were any available step by step protocols on
removing glass coverslips from cells grown on them. I have been
trying to study the en face structure of cells. 1) growing them on
coverslips 2)Processing them with Spurrs for ultramicronomy 3) using
BEEM capsules filled with Spurrs resin and inverting them in cotact
with the glass coverslips 4) After polymerizing, using heat to melt
and remove the coverslip. 5) Section and view them on TEM. So far,
the surface of the cells have been stuck to the coverslip after
removal or glass pieces stuck to the sample.
I am open to suggestions for any other strategy.
Thanks.
Eunice


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 08:47:42 2005



From: Ken Converse :      kenconverse-at-qualityimages.biz
Date: Mon, 14 Mar 2005 09:57:15 -0500
Subject: [Microscopy] magnetic lenses; classroom example

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ellery,
Probably the reason you can't find info on the windings is because the
critical term (after the lens design itself) is ampere-turns. If you look
at some of the very old EMs that used vacuum tubes, the lens supplies were
likely to operate at voltages in excess of 100VDC but currents in the mA
range. They used thousands of turns of very fine wire. Solid state EMs
tend to operate at much lower voltages (24-48VDC) and considerably higher
currents (several amps) so they have far fewer turns of heavier gauge wire.
In other words 10,000 turns at 100 mA will generate roughly the same
magnetic field as 300 turns at 3.3 A. In each case you have 1000
ampere-turns. The wire gauge is determined primarily by how small a
diameter you can get away with given the heat generation at the given
current and what your space constraints are. Some lenses are water or oil
cooled so that finer wire can be used due to space limitations.

It sounds like Paul Grover is offering a complete column, which I can't do
at the present time. If the column falls through, I might be able to offer
an objective or condenser lens.

Some years ago I read someone's story on cross-sectioning an EM column, but
I don't recall where I read it. The main point was that they had completely
embedded the column in epoxy (or other clear liquid material), THEN
sectioned and polished. It's a major project requiring a serious powerfeed
bandsaw and a lot of polishing, but the results would be stunning.

Good luck and post some images if you do it!

Ken Converse

owner 
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
} From: Ellery Frahm [mailto:frah0010-at-tc.umn.edu]
Sent: Friday, March 11, 2005 10:50 AM
To: message to:
Cc: Ellery Frahm

Microscopy folks,

I am always looking for interesting visual aids for the course I teach
on scanning electron microscopy and X-ray microanalysis. For instance,
I have an old WD spectrometer that I bring to lecture, let the student
turn the screw that moves the dispersing crystal and detector, etc.

For lectures on magnetic lenses, I'd like to use either unwound
windings from an old magnetic lens or just an equivalent length and
gauge of copper wire. Unfortunately, it is difficult to find online
suppliers of old or dead electron microscopy components (not much
demand, I suspect), and I cannot find any references (or even rough
estimates) to the number of windings in, say, a typical condenser lens
or what the gauge of that wire would be.

Any help with either of these endeavors would be much appreciated.

Thanks,
Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu







___________________________________________________________
$0 Web Hosting with up to 120MB web space, 1000 MB Transfer
10 Personalized POP and Web E-mail Accounts, and much more.
Signup at www.doteasy.com




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 09:26:43 2005



From: Hong Yi :      hyi-at-emory.edu
Date: Mon, 14 Mar 2005 10:36:37 -0500
Subject: [Microscopy] (Microscopy) Victawet grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you, everyone, for replying to my posting on CCD camera for TEM.
I now have a totally unrelated question.

I got several replies to my previous posting on carbon coated grids.
One of them was about treating the grids with Victawet. I would like
to try this method but the cost is somewhat high if we order from a
vender. So I would like to know more before spending money. I would
very much appreciate it if I can hear the experience from those who
have used this type of grids. Thank you.

HOng
Emory EM



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 09:42:58 2005



From: willis.robert-at-epamail.epa.gov
Date: Mon, 14 Mar 2005 10:51:39 -0500
Subject: [Microscopy] SEM: fiber analysis question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am analyzing dust samples for a specific fiber which has been
suggested as a marker for a particular source of contamination.
Ultimately, I want to relate the concentration of the marker fiber to
the amount of contamination of the dust sample (in weight percent). The
marker fibers vary enormously in length from { 10 microns to } 600
microns, and thus span a huge range in mass. My question is: what
measure of fiber concentration would make the most sense in trying to
correlate fiber concentration with the level of contamination? E.g.,
number concentration (per gram of dust), total fiber length per gram of
dust, total area, or total fiber mass per gram of dust? Fiber
concentration on a mass basis is enormously skewed by a small number of
very long fibers, so I'm thinking that number concentration or total
fiber length may be more appropriate.

Thank you for your suggestions.

******************************************************
Robert Willis, Ph.D.
Science Advisor
Alion Science and Technology
c/o U.S. EPA
Mail Drop E-205-06
Research Triangle Park, NC 27711
Tel: 919-541-2809 Fax: 919-541-3566
willis.robert-at-epa.gov
******************************************************




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 11:09:02 2005



From: Battjes, Kevin :      battjes-at-impactanalytical.com
Date: Mon, 14 Mar 2005 12:18:40 -0500
Subject: [Microscopy] MMM Spring Meeting - Call for Papers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Announcement and Call for Papers
Michigan Microscopy &Microanalysis Society Spring Meeting in 2005

Comfort Inn/University Park Conference Center
Mt. Pleasant, MI
May 20th, 2005

Abstract Deadline: April 22nd, 2005

The Spring Meeting of the Michigan Microscopy and Microanalysis Society will
be held on May 20th, 2005 at the Comfort Inn/University Park Conference
Center in Mt. Pleasant, Michigan. In this one-day conference, there will be
two sessions. One is a platform session having approximately 8-10 speakers
representing industry, academia, and research laboratories. The other is a
poster session. In addition to the speakers and posters, vendors will
exhibit a wide range of products and services of interest to the microscopy
community. Presentations are being solicited from researchers in the
Physical and Biological Sciences, including one vendor presentation and an
invited speaker. Student participation is particularly encouraged. Also,
vendors are encouraged to contact the below address to reserve space for
product display.

Abstract Submission
Please submit a 300 to 350 word abstract by April 22nd indicating which
session you prefer (poster or presentation) to:

Geoff Williams
217 Brooks Hall
Biology Department
Central Michigan University
Mt. Pleasant, MI 48859
Ph 989 774 3576
Fax 989 774 3462
Email: ge.willi-at-cmich.edu {mailto:ge.willi-at-cmich.edu}




Kevin Battjes
Impact Analytical Voice 989-832-5555, ext 556
Michigan Molecular Institute Fax 989-832-5560
1910 W. St Andrews Road e-mail: battjes-at-mmi.org
Midland MI 48640 battjes-at-impactanalytical.com




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 12:28:42 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 14 Mar 2005 12:37:50 -0600
Subject: [Microscopy] viaWWW: removing glass coverslips from cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Eunice,

Another alternative, if someone hasn't already mentioned it, is to grow
the cells on Thermonox plastic coverslips and embed the coverslips
directly into your resin. You can section these like any other sample
and avoid having to remove a glass cover slip from resin. Thermonox
cover slips are designed for cell culture, should be readily available
the various scientific and EM supply houses and come in a variety of
sizes and shapes.

The drawback is that you will most likely have to section the
coverslips, and thus the cells, in cross-section, rather than having the
nice top-down (or bottoms-up, rather) view of the cell. If that's not a
problem, Thermonox is a great help.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu









-----Original Message-----
} From: by way of MicroscopyListserver [mailto:echeung-at-eyetech.com]
Sent: Friday, March 11, 2005 11:03 PM
To: microscopy-at-ns.microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (echeung-at-eyetech.com) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, March 11, 2005 at 17:29:24
------------------------------------------------------------------------
---

Email: echeung-at-eyetech.com
Name: Eunice Cheung

Organization: eyetech

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi.
I was wondering if there were any available step by step protocols on
removing glass coverslips from cells grown on them. I have been trying
to study the en face structure of cells. 1) growing them on coverslips
2)Processing them with Spurrs for ultramicronomy 3) using BEEM capsules
filled with Spurrs resin and inverting them in cotact with the glass
coverslips 4) After polymerizing, using heat to melt and remove the
coverslip. 5) Section and view them on TEM. So far, the surface of the
cells have been stuck to the coverslip after removal or glass pieces
stuck to the sample.
I am open to suggestions for any other strategy.
Thanks.
Eunice


------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 13:24:52 2005



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Mon, 14 Mar 2005 15:30:37 ADT
Subject: [Microscopy] TEM: Spurr and methanol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everybody,
Is there a reason why methanol should not be used as a
dehydrating agent for samples to be embedded in Spurr resin?
Thanks
Dorota


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 13:27:29 2005



From: JoAnn Buchanan :      redhair-at-stanford.edu
Date: Mon, 14 Mar 2005 11:36:28 -0800
Subject: [Microscopy] removing glass coverslips from cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Eunice. In our lab we routinely process hippocampal neurons grown on
glass coverslips for TEM. Since I often have to relocate an individual
neuron that has been imaged by LM, I need to have the entire coverslip
intact so that I can find the cells. I embed in a Chang monolayer mold
using Embed 812 resin. When the coverslips are polymerized, I file down the
edges of the embedded coverslips with a metal file and immerse them in
hydrofluoric acid. The acid is in small plastic beakers kept under the fume
hood.(always wear nitrile gloves) After the coverslips are dissolved
(takes about 20-30 minutes). Remove the the plastic wafer with plastic
forceps and rinse well in running water. You can dry the wafers in the
embedding oven. I then stain them with toludine blue to see the cells in
the light microscope(unless I have done immuno-staining, then I leave them
unstained) to locate the cell of interest. Mark it with a marking pin that
in attached to a microscope objective. You can then punch out the cell
with a leather hole punch and reattach to a blank stub using resin. Trim
down to region of interest.
This way you can get many blocks from 1 coverslip and get a particular cell
if you need to.
If you work under the hood and wear gloves and use plastic containers, you
won't be exposed to the acid. The hydrofluoric acid is available in a
special container equipped with a safety pouring spout (Fisher).This is
more reliable than the popping off method that can leave glass fragments
which are bad for you knife.
Good luck, JoAnn

Department of Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 14:07:28 2005



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 14 Mar 2005 14:17:26 -0600
Subject: [Microscopy] Bone sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

I have a problem sectioning non-demineralized bone. I pick sections on a
carbon coated formvar film, and I am getting multiple wrinkles on areas
with
mineral: http://www.umkc.edu/dentistry/microscopy/index-f.htm
Areas with soft tissue (on the right of the second picture) are good,
no wrinkles.

Any comments?

Thank you,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 14:22:39 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Mon, 14 Mar 2005 15:32:32 -0500
Subject: [Microscopy] viaWWW: removing glass coverslips from cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

That's OK except that some cells will not attach to plastic and need to be grown
on glass (and vice verse).

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Monday, March 14, 2005 1:38 PM
To: by way of MicroscopyListserver
Cc: microscopy-at-microscopy.com

Eunice,

Another alternative, if someone hasn't already mentioned it, is to grow
the cells on Thermonox plastic coverslips and embed the coverslips
directly into your resin. You can section these like any other sample
and avoid having to remove a glass cover slip from resin. Thermonox
cover slips are designed for cell culture, should be readily available
the various scientific and EM supply houses and come in a variety of
sizes and shapes.

The drawback is that you will most likely have to section the
coverslips, and thus the cells, in cross-section, rather than having the
nice top-down (or bottoms-up, rather) view of the cell. If that's not a
problem, Thermonox is a great help.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu









-----Original Message-----
} From: by way of MicroscopyListserver [mailto:echeung-at-eyetech.com]
Sent: Friday, March 11, 2005 11:03 PM
To: microscopy-at-ns.microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (echeung-at-eyetech.com) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, March 11, 2005 at 17:29:24
------------------------------------------------------------------------
---

Email: echeung-at-eyetech.com
Name: Eunice Cheung

Organization: eyetech

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi.
I was wondering if there were any available step by step protocols on
removing glass coverslips from cells grown on them. I have been trying
to study the en face structure of cells. 1) growing them on coverslips
2)Processing them with Spurrs for ultramicronomy 3) using BEEM capsules
filled with Spurrs resin and inverting them in cotact with the glass
coverslips 4) After polymerizing, using heat to melt and remove the
coverslip. 5) Section and view them on TEM. So far, the surface of the
cells have been stuck to the coverslip after removal or glass pieces
stuck to the sample.
I am open to suggestions for any other strategy.
Thanks.
Eunice


------------------------------------------------------------------------
---





From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 21:05:48 2005



From: mikeraj-at-streamyx.com (by way of MicroscopyListserver)
Date: Mon, 14 Mar 2005 21:15:49 -0600
Subject: [Microscopy] viaWWW: dislocation loop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mikeraj-at-streamyx.com) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Sunday, March 13, 2005 at 11:48:59
---------------------------------------------------------------------------

Email: mikeraj-at-streamyx.com
Name: Mike Marks

Organization: Cornell University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi, I understand what a dislocation loop is, but what is a
prismatic dislocation loop ? Thanks !

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 14 21:06:01 2005



From: tbhatt-at-uic.edu (by way of MicroscopyListserver)
Date: Mon, 14 Mar 2005 21:16:11 -0600
Subject: [Microscopy] viaWWW: JB4 microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tbhatt-at-uic.edu) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Monday, March 14, 2005 at 10:48:17
---------------------------------------------------------------------------

Email: tbhatt-at-uic.edu
Name: T. Bhattacharyya

Organization: OTO-HNS

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I am trying to get some info and preliminary training for
using JB4 microtome for plastic resin histology. Within the Chicago
area is there any such laboratory that may be helpful ?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 15 07:52:47 2005



From: John Shields :      jpshield-at-uga.edu
Date: Tue, 15 Mar 2005 09:02:47 -0500
Subject: [Microscopy] SEMS at Florida

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,
The Southeastern Microscopy Society (a LAS of MSA) will be
having its annual meeting May 18-20, 2005 at Pensacola
Beach, Florida.
Invited Speakers include Dr. Mark Farmer from the NSF on NSF
funding opportunities for Instrumentation, Dr. Sara Miller
from Duke Univ. on Lab Preparation for Bioterrorism
Surveillance, Dr. Sandeep Shah of NASA on Microscopy
Techniques in the Investigation of the Space Shuttle
Columbia Accident, and Dr. Thom Hopen of the ATFE on
Forensic Microscopy.

Also included are Tutorials and demonstrations by Microscopy-
related Corporate members such as AETOS, Nikon, Skyscan
MicroCT and Hitachi.

SEMS meetings are always informative, instructive, and
relaxed for a society of its size. We encourage student
participation through the Ruska competition which offers a
monetary award and waivers of registration, banquet fees and
provides rooms for the
participating students.

More information on SEMS, the meeting this May, the Ruska
competition and our newsletter are available at
www.semicroscopy.org

Looking forward to seeing you there!
John Shields
--
John Shields
E.M. Lab, 151 Barrow Hall
University of Georgia
Athens, GA 30602
706-542-4080

John P. Shields
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602

706-542-4080


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 15 12:54:21 2005



From: Jon Mulholland :      jwm-at-stanford.edu
Date: Tue, 15 Mar 2005 11:04:11 -0800
Subject: [Microscopy] LSM510 IRM contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
We would like to use our LSM510 confocal to do reflection
interference contrasting (which we have no experience with). However, we
are not sure about the equipment we would need for this imaging technique
and if it can be applied to the confocal. As I understand it, the
technique normally requires, when using Hg illumination, an illumination
source polarizer, antiflex objective (which would have the quarter-wave
retardation plate mounted on the lens) and also an analyzer. We would be
using laser excitation and so it will already be polarized and we can
obviously setup the scope for reflective microscopy, we also have a Zeiss
63X antiflex lens.
What we don't have is the analyzer and an understanding of where
it would be placed in the 510 light path so it could work. Does anyone have
experience using the technique on a confocal, is it possible?

Jon Mulholland
Cell Sciences Imaging Facility
Beckman Center, B050
Stanford University School of Medicine
Stanford, CA 94305-5301

voice 650-725-7532
fax 650-725-4951

http://taltos.stanford.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 15 13:01:52 2005



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Tue, 15 Mar 2005 14:11:40 -0500
Subject: [Microscopy] Dye for Acrylic Adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

List:

I am trying to locate a dye that will allow me to detect adhesive creep in
an acrylic adhesive (pressure type) label system. I know that metal stains,
OsO4 & RuO4, can be used on acrylic adhesives, however, we are looking for
other approaches to advance our research. If you have any thoughts or
suggestions I would appreciate the assistance. Thank you, jr

John A. Robson





From MicroscopyL-request-at-ns.microscopy.com Tue Mar 15 13:55:52 2005



From: Alan Nicholls :      nicholls-at-uic.edu
Date: Tue, 15 Mar 2005 14:08:18 -0600
Subject: [Microscopy] MMMS Meeting March 24th, 2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Local MSA, MAS affiliate meeting announcement

For further information and abstracts for the student poster competition go
to:-
http://www.northwestern.edu/bioimaging/MMMS%20Website/meetings.htm

Imaging Biomolecular Interactions
Midwest Microscopy and Microanalysis Society
Co-sponsor: Biological Imaging Facility, Northwestern University

Thursday, March 24, 2005

Northwestern University
Pancoe Life Sciences Building
Pancoe/ENH Auditorium
Evanston, IL 60208

Meeting Schedule
Morning Session
9:00 Registration
9:45 Welcome and introduction
10:00 Victoria Froelich, University of Texas Health Sciences Center San
Antonio: FRET, dectection of molecular interactions.
11:00 Kanya Rajangam, Northwestern University: Cells in self-assembling gels.
11:30 Carina Holmberg and Heather Brignull, Northwestern University:
Imaging-based characterization of aggregates in neurodegenerative disease
models.
12:00-12:45 Lunch
12:45 Business Meeting

Afternoon session
1:00 H. Peter Lu, Paci. c Northwest National Laboratory: Single-molecule
biophysics.
2:00 Debbie Klos, Northwestern University: Recognition of membrane protein
cargo by the Rsp5 ubiquitin ligase.
2:30 Victoria Wu, Northwestern University: The expansive tube, the role of
the septate junction in Drosophila tracheal development.
3:00 Reception and Student Poster Competition Exhibit and Judging.

RSVP Required
Please send RSVP via email, phone, or fax to:
Arvid Casler, MMMS Program Coordinator
c/o MAI
P. O. Box 394
Mundelein, IL 60060
phone/FAX: (847) 566-7716
email: arvid_casler-at-fmo.com


Admission: Free to MMMS Members
MMMS Membership: $10.00
MMMS membership available at registration

Driving directions to Northwestern University Evanston Campus available on
the Web.
From the north or northwest, via Interstate 88, Interstate 90 or
Interstate 190
(Note: these are the directions to follow if traveling from O'Hare
International Airport)
http://www.northwestern.edu/campus/directions/evanston-north-northwest.html
From the west, via Interstate 94
http://www.northwestern.edu/campus/directions/evanston-west.html
From the west or southwest, via Interstate 55 or Interstate 80
http://www.northwestern.edu/campus/directions/evanston-west-southwest.html
From the south or southeast, via Interstate 94, Interstate 90, Interstate
80 or Interstate 57
http://www.northwestern.edu/campus/directions/evanston-south-southeast.html

PARKING
Parking permits are $4 and are nonrefundable.
Parking can be challenging on the NU Evanston Campus especially after 9AM.
Spaces in selected lots are available on a first-come-first-served basis.



Alan W Nicholls, PhD
Newsletter Editor - M3S
Research Resources Centre - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago IL 60607-7058

Office: Room 110
Tel: 312 996 1227
Fax: 312 996 8091
nicholls-at-uic.edu
http://www.northwestern.edu/bioimaging/MMMS%20Website/index.htm



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 15 15:11:44 2005



From: Elaine F. Schumacher :      eschumacher-at-mccrone.com
Date: Tue, 15 Mar 2005 15:19:06 -0600
Subject: [Microscopy] Dye for Acrylic Adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

Would a fluorescent dye that's soluble in your adhesive, and
fluorescence microscopy be of help to you? We've had success with
fluorescent dyes to track components such as fillers and adhesives in
several projects here.

Regards,

Elaine

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************

-----Original Message-----
} From: jrobson-at-rdg.boehringer-ingelheim.com
[mailto:jrobson-at-rdg.boehringer-ingelheim.com]
Sent: Tuesday, March 15, 2005 1:12 PM
To: Microscopy-at-MSA.Microscopy.Com

List:

I am trying to locate a dye that will allow me to detect adhesive creep
in
an acrylic adhesive (pressure type) label system. I know that metal
stains,
OsO4 & RuO4, can be used on acrylic adhesives, however, we are looking
for
other approaches to advance our research. If you have any thoughts or
suggestions I would appreciate the assistance. Thank you, jr

John A. Robson







From MicroscopyL-request-at-ns.microscopy.com Wed Mar 16 10:55:35 2005



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Wed, 16 Mar 2005 11:04:54 -0600
Subject: [Microscopy] Position Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,
The ITG microscopist search has officially opened. The announcement is
posted at http://www.itg.uiuc.edu/announcements/VisitMicro.htm. Glen
Fried (gfried-at-itg.uiuc.edu; Office Phone: (217) 333-5493 ) or myself
(contact info. below) can be contacted if more information is desired.
I've appended the details below:

Applicants are being sought for the position of Visiting Microscopist in
the Imaging Technology Group (ITG) at the Beckman Institute at the
University of Illinois at Urbana-Champaign. The ITG provides both a
visualization facility and microscopy facility for campus researchers
with interests ranging from physics to biology and the arts. The
Microscopy Suite provides a wide selection of electron, scanning probe,
and optical imaging capabilities. Optical microscopy capabilities
include laser scanning confocal microscopy, multiphoton microscopy,
widefield transmitted and fluorescence microscopy, stereomicroscopy,
computer-assisted stereology, near-field scanning microscopy,
reflected/transmitted light micro-spectroscopy, and near IR imaging.
Further information can be found at
http://www.itg.uiuc.edu/ms/equipment/. The responsibilities of the
Visiting Microscopist include, but are not limited to:**

* Training new users, and assisting existing users with confocal
microscopy.
* Maintaining and benchmarking the performance of the confocal
microscope and following up on service calls.
* Training and assisting users with fluorescence, stereology, and
bright field imaging.
* Acting as a secondary resource for training and assisting users on
the electron and scanning probe microscopes.
* Develop advanced imaging technologies utilizing the capabilities
present in the ITG.
* Assisting in the evaluation, procurement, installation, and
maintenance of new instruments.
* Trouble shooting and user assistance on ancillary equipment.
* Maintaining facility equipment.
* Other duties as assigned.

Qualifications: Bachelor’s degree, two-years experience in optical
microscopy, and excellent verbal and written communications skills are
required. A strong background in biology and/or materials
characterization, experience in a microscopy user facility, knowledge
and/or experience in electron and scanning-probe microscopy, and an
advanced degree are preferred.

This is a full-time, visiting academic professional position with
university benefits and with the possibility of transitioning to a
permanent position. The start date is as soon as possible following the
close of the search. Salary is commensurate with qualifications and
experience. In order to ensure full consideration, applications must be
received by April 22, 2005. Applicants may be interviewed before the
closing date; however, no hiring decisions will be made until after that
date. To apply please send a letter of interest, resume, three letters
of reference, and contact information to:

Lori Heil
Beckman Institute
405 North Mathews Avenue
Urbana, IL 61801
Phone: 217-244-0170
Fax: 217-244-6219
E-mail: lheil-at-uiuc.edu

*/ The University of Illinois is an Affirmative Action, Equal
Opportunity Employer./*



--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Office: B650J
Phone: 217.244.6292
Fax: 217.244.6219
Mobile: 217.390.1874
www.itg.uiuc.edu



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 16 12:06:19 2005



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 16 Mar 2005 13:15:25 -0500
Subject: [Microscopy] Agfa DuoScan 2500 bulb Source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

After about 4 years of solid use our scanner bulb seems to be failing.
 However I’m coming up empty on replacement sourcing.  And there is still a
small bit of doubt if it is the transmitted lamp at all anyway.
 
I’ve disassembled it enough to evaluate that it is not a trivial matter to
replace the bulb but that it should be possible.
 
My question to the group is: has anyone replaced a transmitted lamp on this
scanner (Agfa DuoScan T2500)?
And (as it appears) assuming this is as unavailable as it appears, what is
the current scanner on the market that is able to generate equivalent TEM
negative scans?
 
I know scanners have been discussed ad-nauseum, but the requirements I’m
looking for are: available and current, (and hopefully less expensive than
the Agfa).  Is the front runner still the Epson Perfection 4870?
 
Thanks!


Geoff Williams
Microscopy Facility Supervisor
 
Central Michigan University Biology Department Microscopy Facility
http://www.cst.cmich.edu/centers/microscopy/





From MicroscopyL-request-at-ns.microscopy.com Wed Mar 16 14:18:27 2005



From: Nancy Smythe :      smythen-at-musc.edu
Date: Wed, 16 Mar 2005 15:27:45 -0500
Subject: [Microscopy] Re: scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been using the Epson Perfection 4870 for almost a year now and am very happy with the results... Much less expensive than the Agfa and faster. It has served us well.



Nancy Smythe
Department of Otolaryngology
Head and Neck Surgery
Medical University of South Carolina
843-792-8835
843-792-0368 Fax




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 16 14:56:23 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Wed, 16 Mar 2005 16:06:09 -0500
Subject: [Microscopy] Histology/sections not adhering

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A colleague of mine was asked to section and stain Axolotl testis
(amphibian testis can be cut such that all stages of spermatogenesis
are present in the section). She was given 2 sets of tissue: ones
fixed in Bouin's (Formalin, glacial acetic acid and picric acid),
the others were fixed in Flemmings solution ( chromic acid, glacial
acetic acid and osmium tetroxide). The sections from the Bouin's
fixed tissue cut nicely but no matter what she has tried, they slip
off of the slides either during the de-paraffinization or
re-hydration steps. She has tried super-frost Plus slides,
gelatin-chrom alum, and even gelatin-chrome alum plus poly-l-lysine.
She lets the sections dry on the slide warming tray for a minimum of
24 hours. These slides are desired for the medical students here,
so she needs to cut and stain 120-150 slides. Very hard to do if you
can't get the sections to stick! She is about to try the
Flemmings-fixed tissue, but we just can't figure out what's going on
with the Bouins-fixed stuff.

Any ideas?

TIA,
Lee
--
Lee Cohen-Gould
Electron & Optical Microscopy Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 09:53:03 2005



From: Jan Factor :      jfactor-at-ns.purchase.edu
Date: Wed, 16 Mar 2005 16:06:09 -0500
Subject: [Microscopy] Histology/sections not adhering

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Years ago I did extensive serial sectioning of Bouin's-fixed,
paraffin-embedded tissues. I had very good results (i.e., sections did
not fall off slides during processing) using a light coating of albumen
fixative on plain, alcohol-cleaned microslides. Good luck.
--Jan

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College
State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------


-------- Original Message --------

A colleague of mine was asked to section and stain Axolotl testis
(amphibian testis can be cut such that all stages of spermatogenesis
are present in the section). She was given 2 sets of tissue: ones
fixed in Bouin's (Formalin, glacial acetic acid and picric acid),
the others were fixed in Flemmings solution ( chromic acid, glacial
acetic acid and osmium tetroxide). The sections from the Bouin's
fixed tissue cut nicely but no matter what she has tried, they slip
off of the slides either during the de-paraffinization or
re-hydration steps. She has tried super-frost Plus slides,
gelatin-chrom alum, and even gelatin-chrome alum plus poly-l-lysine.
She lets the sections dry on the slide warming tray for a minimum of
24 hours. These slides are desired for the medical students here,
so she needs to cut and stain 120-150 slides. Very hard to do if you
can't get the sections to stick! She is about to try the
Flemmings-fixed tissue, but we just can't figure out what's going on
with the Bouins-fixed stuff.

Any ideas?

TIA,
Lee
--
Lee Cohen-Gould
Electron & Optical Microscopy Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207


--




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 10:37:27 2005



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Thu, 17 Mar 2005 11:34:28 -0500
Subject: [Microscopy] Re: Histology/sections not adhering

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} A colleague of mine was asked to section and stain Axolotl testis
} (amphibian testis can be cut such that all stages of spermatogenesis
} are present in the section). She was given 2 sets of tissue: ones
} fixed in Bouin's (Formalin, glacial acetic acid and picric acid),
} the others were fixed in Flemmings solution ( chromic acid, glacial
} acetic acid and osmium tetroxide). The sections from the Bouin's
} fixed tissue cut nicely but no matter what she has tried, they slip
} off of the slides either during the de-paraffinization or
} re-hydration steps. She has tried super-frost Plus slides,
} gelatin-chrom alum, and even gelatin-chrome alum plus poly-l-lysine.
} She lets the sections dry on the slide warming tray for a minimum of
} 24 hours. These slides are desired for the medical students here,
} so she needs to cut and stain 120-150 slides. Very hard to do if
} you can't get the sections to stick! She is about to try the
} Flemmings-fixed tissue, but we just can't figure out
Lee Cohen-Gould lcgould-at-med.cornell.edu
====================
Lee,
Bouin's fixation makes the tissue very hard, if I remember correctly.
This could make a difference in the tissue's adherence to the slides.

As to making the tissue stick - I have a technique that I learned
many years back when
I was attempting to make LM serial sections of lung tissue for a
reconstruction study.
I had tried every subbing solution and slide cleaning technique that
I could find in all
the histology books that I had. I also tried slides from 3 or 4
different companies.
Many sections would disappear at different times during the staining procedure.

Then I remembered Dorothy, a very experienced histology tech who
worked in the same lab
as I had several years before. I had never seen loose a paraffin
section! I went to her lab to observe just what she did differently
than I did from the time she put her coat on the hook
until she took a pinch of gelatin out of a rather large brown bottle
and she just sprinkled it
on top of the cold water that was in the water bath that would be
used to float the sections
when it had warmed to the proper temperature.
That was it! A pinch of gelatin - no subbing - no washing of the slides -
and I did not loose another section.

I asked Dorothy where she had learned the trick but she could not remember.
She thought that everyone knew about the gelatin in the water bath.
I guess that it is similar to learning electron microscopy techniques
from a book
without spending some time watching or talking with someone who has
things working.

Hoping it works for those in need!
Pat Connelly psconnel-at-sas.upenn.edu
The University of Pennsylvania
Department of Biology
Philadelphia, PA 19104-6018
215-898-7145


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 12:23:34 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 17 Mar 2005 13:34:06 -0800
Subject: [Microscopy] Re: Histology/sections not adhering

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Lee:

I suspect that his/her slides were not clean before the adhesive was
applied. In my many years of experience, slides out of the box are not
really clean, they have a very thin film of ?? on them. This film can be
removed by soapy water or alcohol. When such 'unclean' slides are coated
with your favorite adhesive, the adhesive lies on the film. When the
slides hit alcohols, the film, the adhesive and the section all come
off. When I started in microtechnique slides were obviously 'unclean'
so we always cleaned them with alcohol before applying egg albumin. Now
I wash my slides (in a slide staining rack) in hot, soapy water, rinse
well, rinse in distilled and dry in a clean (not used for wax) oven .
Then I coat with subbing soln, poly-L-lysine or 'silane' as needed. I
almost never lose sections.
Also, I always allow the water to drain from under the section by
proping the slide up (almost vertical) against the side of the warming
plate for a minute or two before laying the slide flat. Sections are
much more likely to lie flat after that. Sections that are flat look
translucent, sections with air under them look opaque and will come off.
Other than the above I know of no good reason for Bouin-fixed
material to not stick to a slide. If the Bouin material won't stick I
suspect the Flemming material won't either.

Geoff

Leona Cohen-Gould wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} A colleague of mine was asked to section and stain Axolotl testis
} (amphibian testis can be cut such that all stages of spermatogenesis
} are present in the section). She was given 2 sets of tissue: ones
} fixed in Bouin's (Formalin, glacial acetic acid and picric acid), the
} others were fixed in Flemmings solution ( chromic acid, glacial acetic
} acid and osmium tetroxide). The sections from the Bouin's fixed
} tissue cut nicely but no matter what she has tried, they slip off of
} the slides either during the de-paraffinization or re-hydration
} steps. She has tried super-frost Plus slides, gelatin-chrom alum, and
} even gelatin-chrome alum plus poly-l-lysine. She lets the sections dry
} on the slide warming tray for a minimum of 24 hours. These slides
} are desired for the medical students here, so she needs to cut and
} stain 120-150 slides. Very hard to do if you can't get the sections
} to stick! She is about to try the Flemmings-fixed tissue, but we just
} can't figure out what's going on with the Bouins-fixed stuff.
}
} Any ideas?
}
} TIA,
} Lee


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 13:41:31 2005



From: kssim-at-mmu.edu.my (by way of MicroscopyListserver)
Date: Thu, 17 Mar 2005 13:51:44 -0600
Subject: [Microscopy] viaWWW: donate one SEM to my lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kssim-at-mmu.edu.my) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, March 15, 2005 at 09:39:40
---------------------------------------------------------------------------

Email: kssim-at-mmu.edu.my
Name: kssim

Organization: mmu

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear All,

I am looking for someone who can donate one SEM to my lab at Malaysia. Please kindly let me know if you have the workable SEM that you wish to donate.

Thank you
Kok Swee, Sim


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 13:40:57 2005



From: Austin.Elliott-at-manchester.ac.uk (by way of MicroscopyListserver)
Date: Thu, 17 Mar 2005 13:51:07 -0600
Subject: [Microscopy] viaWWW: Low temperature stages/chambers for light microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Austin.Elliott-at-manchester.ac.uk) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, March 15, 2005 at 06:15:58
---------------------------------------------------------------------------

Email: Austin.Elliott-at-manchester.ac.uk
Name: Austin Elliott

Organization: University of Manchester UK

Title-Subject: [Microscopy] [Filtered] Low temperature stages/chambers for light microscopy

Question: My first encouter with this forum...

Does anyone know any good COMMERCIALLY-AVAILABLE low-temperature stages or (perhaps better) "environmental chambers" to do low temperature light microscopy on inverted microscopes?

Brief B/G - I have been working for many years with live-cell fluorescence/light microscopy/imaging on inverted 'scopes - all at room temperature! I have been reading a lot of fascinating stuff about cell freezing/ cryopreservation. Microscopy has been used to investigate this area, and a bunch of systems to produce a temp-controlled cryochamber for cells on the stage have been described, but all are basically one-off designs requiring a fair bit of engineering. I was wondering if there was anything commercially available.

Thanks

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 13:41:44 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 17 Mar 2005 14:51:34 -0500
Subject: [Microscopy] Re: Histology/sections not adhering

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks again to everyone who responded. The gist of the advice is to
clean your slides (we did), and try one of the following adhesives
until you find one that works:
-good old fashioned egg albumin
-gel-chrom alum
-0.1% aqueous polyethyleneimine
-poly-l-lysine
-silane

thanks for the suggestion of trying different cleaning methods
(alcohol, acid or bleach) for different results.
I'll pass all of this along to my colleague and will be glad that I'm
not the one who has to try the various permutations of all of this
(this time).

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 13:42:50 2005



From: Pietra-philipp-at-cooperhealth.edu (by way of MicroscopyListserver)
Date: Thu, 17 Mar 2005 13:52:47 -0600
Subject: [Microscopy] viaWWW: Kodak technical pan film 6415 discontinued

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Pietra-philipp-at-cooperhealth.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, March 16, 2005 at 11:13:36
---------------------------------------------------------------------------

Email: Pietra-philipp-at-cooperhealth.edu
Name: Philipp Pietra

Organization: Cooper university hospital department of Pathology

Title-Subject: [Microscopy] [Filtered] MListserver:Kodak technical pan film 6415 discontinued

Question: Does any one have any recommendations for substitute film since Kodak has discontinued their technical pan film 6415. I am using Zeiss TEM109 with transfiberoptic imaging camera using the 120mm film size. Any one else with this set up your input is valuable to me. EMS and Kodak have recommended the T-max 100 as a replacement, but for all my playing around I can't get the contrast to what I need. I am doing routine pathology and routinely there is no money so a new scope with a fancy digital camera is out of the question.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 13:42:19 2005



From: lilia.zhahalyak-at-trincoll.edu (by way of MicroscopyListserver)
Date: Thu, 17 Mar 2005 13:52:17 -0600
Subject: [Microscopy] viaWWW: TEM lab manual for cell (ventral mesencephanol) monolayer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (lilia.zhahalyak-at-trincoll.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, March 15, 2005 at 12:34:53
---------------------------------------------------------------------------

Email: lilia.zhahalyak-at-trincoll.edu
Name: lilia zhahalyak

Organization: Trinity college, hartford, ct

Title-Subject: [Microscopy] [Filtered] MListserver: TEM lab manual for cell (ventral mesencephanol) monolayer

Question: I am looking for a scientific reference ie lab manuals for TEM primary cell culture with detailed steps of the procedure. I have Elaine Hunter "Practical electron Microscopy" 2nd edition, but it is not enough material for monolayer, and the procedure is rather vague. I even contucted M.A. Hayat, who wrote several books on the microscopy methods, but I do not think that he has time to unswer me. Do you have any suggestions?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 13:43:14 2005



From: jcai-at-nanostellar.com (by way of MicroscopyListserver)
Date: Thu, 17 Mar 2005 13:53:27 -0600
Subject: [Microscopy] viaWWW: electron diffraction pattern to study nanoparticle size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jcai-at-nanostellar.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, March 16, 2005 at 16:35:23
---------------------------------------------------------------------------

Email: jcai-at-nanostellar.com
Name: Juan

Title-Subject: [Microscopy] [Filtered] electron diffraction pattern to study nanoparticle size

Question: Dear listers:



I am wondering is there a well-established method to analyze nano-particle size from the electron diffraction pattern. For example, in the polycrystalline materials, can the full width of the half maximum of a diffraction spot be converted to the particle size? Is the Sherrer equation applicable in the electron diffraction analysis?



Any information is appreciated. Thanks.



Juan


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From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 13:43:50 2005



From: Stacey.Andringa-at-uc.edu (by way of MicroscopyListserver)
Date: Thu, 17 Mar 2005 13:54:02 -0600
Subject: [Microscopy] viaWWW: ISEL technique to assay for apoptosis-related DNA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, March 17, 2005 at 06:31:11
---------------------------------------------------------------------------

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: A colleague of mine has a question about the ISEL technique to assay for apoptosis-related DNA fragmentation. He is unable to find any "kits" for this technique. He is interested in the ISEL procedure because he was told that it is better than TUNEL when assaying epithelial tissue/cells. The tissues being analyzed are stratified squamous epithelia.

Is it indeed true that ISEL is preferable to TUNEL and
are there validated protocols out there for mouse back-skin and forestomach?

Thanks for any help.

Stacey Andringa






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From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 13:44:23 2005



From: kweidenh-at-montefiore.org (by way of MicroscopyListserver)
Date: Thu, 17 Mar 2005 13:54:34 -0600
Subject: [Microscopy] viaWWW: uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kweidenh-at-montefiore.org) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, March 17, 2005 at 12:12:00
---------------------------------------------------------------------------

Email: kweidenh-at-montefiore.org
Name: Karen Weidenheim

Organization: Montefiore Medical Center/Albert Einstein Coll Med

Title-Subject: [Microscopy] [Filtered] "new" uranyl acetate problem

Question: Dear Colleagues, since 11/04, upon receiving a new lot of UA, we have not been able to obtain usable contrast on diagnostic tissue specimens. We had been using standard UA/lead citrate staining with no problems for the 7 years I have been running the lab. Since 11/04, we have tried multiple permutations of ethanol/water solutions, changed the water we use several times, changed staining times (several times) all to no avail. WE are working with EM sciences; while the situation is murky, it sounds like the formulation of the UA salt has been changed without asking persons that actually use it, at least for diagnostic purposes.
I can no longer do diagnostic em work on anything other than low power kidney bx because there is insufficient contrast to delineate the subcellular organelle structures that I need to see, i.e. mitochondria, thick and thin filaments, etc. I cannot even find the area of the grid that may be pathologic based on my review of the screening sections. I get eyestrain on the scope because I cannot see.
Does anyone else have the same problem with this "new" reagent? and if so what have you done about it.


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From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 14:59:54 2005



From: Karl Hagglund :      hagglundk1-at-nku.edu
Date: Thu, 17 Mar 2005 16:09:48 -0500
Subject: [Microscopy] Hitachi s-570 maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues,

I am in the process of cleaning the column and apertures on an Hitachi
S-570 SEM. I have found most of the necessary tools, but cannot find
something called the fixed aperture take off tool. I have checked my
manual, and it lists two parts as aperture tools (tool for obj. aperture
exchange, and tool for obj. aperture set). I have photos of these, and
have both in my tool kit. Unfortunately, neither appears to be what is
pictured in the manual.

I have posted the diagram of the tool and its use at the following URL:

http://semlab.nku.edu/aperturetool.html

Does anyone out there have one of these that might be available?
Otherwise, if someone could tell us what it is called (or even a part
number). I cannot find anything called the fixed aperture take-off tool
in my parts list or manual. I am down to the last aperture on the
system, and have quite a stigmation problem that I am thinking is a
result of this one. I would love to get this microscope up and running
at its original potential again.

Any tips on how to rig something that would work in lieu of the tool
would be appreciated as well.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 17:50:39 2005



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 17 Mar 2005 17:59:21 -0600
Subject: [Microscopy] RE: Hitachi s-570 maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Look instead for a tube with a rod through the center from one end and a
fine internal thread on the other end. That final aperture is threaded on
the outside, so the tube is placed up against it, threaded on and pulled
down to release the holder. When putting it on, thread it on the tube,
push it in place and use the rod to apply pressure on it while unthreading
the tube.

The holder is just held in place by a compression ring and can easily be
removed without a tool. Strong fingernails can do it, or the wooden end of
a swab can be carefully placed in the opening, pushed sideways so that you
get some friction in the hole and then pulled down.

I haven't seen the tool you had a diagram of before. I've worked on a
number of 570s and they have always had the tube and rod tool I've tried to
describe.

Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
Saint Charles, Illinois 60174
phone (630) 513-7093 fax (630) 513-7092
email mailto:ars-at-sem.com web www.sem.com


On Thursday, March 17, 2005 3:10 PM, Karl Hagglund
[SMTP:hagglundk1-at-nku.edu] wrote:
}
}
}
------------------------------------------------------------------------
------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
-------
}
} Colleagues,
}
} I am in the process of cleaning the column and apertures on an Hitachi
} S-570 SEM. I have found most of the necessary tools, but cannot find
} something called the fixed aperture take off tool. I have checked my
} manual, and it lists two parts as aperture tools (tool for obj. aperture
} exchange, and tool for obj. aperture set). I have photos of these, and
} have both in my tool kit. Unfortunately, neither appears to be what is
} pictured in the manual.
}
} I have posted the diagram of the tool and its use at the following URL:
}
} http://semlab.nku.edu/aperturetool.html
}
} Does anyone out there have one of these that might be available?
} Otherwise, if someone could tell us what it is called (or even a part
} number). I cannot find anything called the fixed aperture take-off tool
} in my parts list or manual. I am down to the last aperture on the
} system, and have quite a stigmation problem that I am thinking is a
} result of this one. I would love to get this microscope up and running
} at its original potential again.
}
} Any tips on how to rig something that would work in lieu of the tool
} would be appreciated as well.
}
} Karl Hagglund
} Biological Sciences, SC-102B
} Northern Kentucky University, Nunn Drive
} Highland Heights, KY 41099
} 859-572-5238
} http://semlab.nku.edu
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 17 18:13:08 2005



From: Lehman, Ann R :      Ann.Lehman-at-trincoll.edu
Date: Fri, 18 Mar 2005 09:30:00 -0500
Subject: [Microscopy] __ viaWWW: TEM lab manual for cell (ventral

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,
Here is my reply to Karl. I have removed the attachment from the Microscopy
Listserver copy.
MM
----- Original Message -----
} From: "Mary Mager" {mager-at-interchange.ubc.ca}
To: "Karl Hagglund" {hagglundk1-at-nku.edu}
Sent: Thursday, March 17, 2005 4:19 PM

Dear Lilia,

Why don't you drop by the EM Library (or see
http://www.trincoll.edu/~alehman/EM_Library.htm) where you could check
out the reference sources Trinity has on-site?? As I've told you, I
would be glad to help if we plan to set up some time together.

In summary, there are several widely-used routine techniques for dealing
with cultured cells. You need to be specific about whether you must
maintain a certain orientation. For example, do you want to examine the
points of attachment of the cells to their substrate, or would a random
sampling of cell orientations suffice? Also, what specifically do you
wish to study? Certain fixation protocols may be used to optimize
traditional morphology, others optimize preservation of immunologically
active sites, while still others can be used to track progressive
changes in cell constituents over time. As an alternative to standard
chemical-based protocols, you can use cryopreservation and/or
cryoultramicrotomy.

Good luck in your search. I don't doubt that someone on this List can
help you with your specific query. Otherwise, I am available during the
Spring Break next week, and my schedule opens up wide thereafter.

Prof L

Ann Hein Lehman
Assistant Director, Electron Microscopy Facility
Trinity College
300 Summit Street
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman


-----Original Message-----
} From: by way of MicroscopyListserver
[mailto:lilia.zhahalyak-at-trincoll.edu]
Sent: Thursday, March 17, 2005 2:52 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (lilia.zhahalyak-at-trincoll.edu) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, March 15, 2005 at 12:34:53
------------------------------------------------------------------------
---

Email: lilia.zhahalyak-at-trincoll.edu
Name: lilia zhahalyak

Organization: Trinity college, hartford, ct

Title-Subject: [Microscopy] [Filtered] MListserver: TEM lab manual for
cell (ventral mesencephanol) monolayer

Question: I am looking for a scientific reference ie lab manuals for TEM
primary cell culture with detailed steps of the procedure. I have
Elaine Hunter "Practical electron Microscopy" 2nd edition, but it is not
enough material for monolayer, and the procedure is rather vague. I even
contucted M.A. Hayat, who wrote several books on the microscopy methods,
but I do not think that he has time to unswer me. Do you have any
suggestions?

------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 18 08:59:20 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Fri, 18 Mar 2005 10:09:41 -0500
Subject: [Microscopy] Re: Histology/sections not adhering

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

At one time, I used to use a product, Histostik, which was sold by Accurate
Chemical & Scientific Corp. (Westbury, NY). The procedure was as follows:

1. Agitate slides in 95% alcohol, then either wipe dry or drain dry.
2. Prepare Histostik solution as follows:
a. Add 6 drops of Histostik to 200ml of deionized or distilled water.
b. Shake and store in a brown bottle at 4 degrees C.
3. Immerse slide(s) into a staining dish containing Histostik solution for
3-5 seconds. Remove and allow slides to drain
dry.
4. Store coated Histostik slides in a dust free plastic slide box.

The procedure worked very well. Bottom line for any procedure: you need to
start with really clean slides.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: Leona Cohen-Gould [mailto:lcgould-at-med.cornell.edu]
Sent: Thursday, March 17, 2005 2:52 PM
To: microscopy-at-msa.microscopy.com

Thanks again to everyone who responded. The gist of the advice is to
clean your slides (we did), and try one of the following adhesives
until you find one that works:
-good old fashioned egg albumin
-gel-chrom alum
-0.1% aqueous polyethyleneimine
-poly-l-lysine
-silane

thanks for the suggestion of trying different cleaning methods
(alcohol, acid or bleach) for different results.
I'll pass all of this along to my colleague and will be glad that I'm
not the one who has to try the various permutations of all of this
(this time).

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 18 09:46:18 2005



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 18 Mar 2005 10:56:08 -0500
Subject: [Microscopy] viaWWW: electron diffraction pattern to study

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In my opinion, there are too many microscope parameters that will affect the width of the diffraction spots such as exposure, convergence angle, intermediate lens setting, condenser aperture for you to be able to do this with electron diffraction. However, the image mode of the TEM is available and it can be calibrated easily enough. (I recommend the Mag-I-Cal sample.) Why not use that. You do have to develop a method to properly disperse your samples on a grid.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
P. O. Box 11472 (letters)
Guys Run Rd. (packages)
Pittsburgh, PA 15238-0472
Walck-at-PPG.com
(412) 820-8651 (office)
(412) 820-8515 (fax)


-----Original Message-----
} From: by way of MicroscopyListserver [mailto:jcai-at-nanostellar.com]
Sent: Thursday, March 17, 2005 2:53 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jcai-at-nanostellar.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, March 16, 2005 at 16:35:23
---------------------------------------------------------------------------

Email: jcai-at-nanostellar.com
Name: Juan

Title-Subject: [Microscopy] [Filtered] electron diffraction pattern to study nanoparticle size

Question: Dear listers:



I am wondering is there a well-established method to analyze nano-particle size from the electron diffraction pattern. For example, in the polycrystalline materials, can the full width of the half maximum of a diffraction spot be converted to the particle size? Is the Sherrer equation applicable in the electron diffraction analysis?



Any information is appreciated. Thanks.



Juan


---------------------------------------------------------------------------




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 18 10:25:54 2005



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Fri, 18 Mar 2005 11:34:35 -0500
Subject: [Microscopy] Re: viaWWW: uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Karen,
We have experienced the same understaining as you. We also obtain our UA
from EMS. After several unsuccessful attempts to get better staining by
increasing UA concentration, temperature, time etc..., we called Stacey
Kirsch. She told us that the UA was further depleted to be a safer product
for users. We discovered the new product was not staining as well as the
old. Stacey sent us two test samples that were dried differently to
increase staining ability (she also sent these test samples to other labs
for comparison). We found both were better, but preferred one over the
other. We are now using the new UA at an 8% aqueous solution for 20
minutes at room temperature followed by 3 minutes in lead citrate. Our old
staining protol called for 5% UA in 50% ethanol for 20 minutes at room
temperature. Stacey told me that she has been supplying this revised
formulation since February 1. It should be the same UA that you are
using. If you wish, we can give you some UA from our stock.

Recently someone posted a staining protocol on the listserver using
iso-butanol saturated water. It smells awful, but shows promise for
increased staining in our preliminary tests. You may want to try this:
I.M. Roberts, Journal of Microscopy
207:97 (2002).

I hope this helps,
Frank



} Email: kweidenh-at-montefiore.org
} Name: Karen Weidenheim
}
} Organization: Montefiore Medical Center/Albert Einstein Coll Med
}
} Title-Subject: [Microscopy] [Filtered] "new" uranyl acetate problem
}
} Question: Dear Colleagues, since 11/04, upon receiving a new lot of UA, we
} have not been able to obtain usable contrast on diagnostic tissue
} specimens. We had been using standard UA/lead citrate staining with no
} problems for the 7 years I have been running the lab. Since 11/04, we
} have tried multiple permutations of ethanol/water solutions, changed the
} water we use several times, changed staining times (several times) all to
} no avail. WE are working with EM sciences; while the situation is murky,
} it sounds like the formulation of the UA salt has been changed without
} asking persons that actually use it, at least for diagnostic purposes.
} I can no longer do diagnostic em work on anything other than low power
} kidney bx because there is insufficient contrast to delineate the
} subcellular organelle structures that I need to see, i.e. mitochondria,
} thick and thin filaments, etc. I cannot even find the area of the grid
} that may be pathologic based on my review of the screening sections. I
} get eyestrain on the scope because I cannot see.
} Does anyone else have the same problem with this "new" reagent? and if so
} what have you done about it.
}
}
} ---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Fri Mar 18 12:46:10 2005



From: Fred Pearson :      eoptics-at-mcmaster.ca
Date: Fri, 18 Mar 2005 13:57:14 -0500 (Eastern Standard Time)
Subject: [Microscopy] New Deadline for MSC2005 abstract submissions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear MSC-SCM members,

Please note that the date for abstract submissions to the May conference has been extended.
Please make sure you submit your abstracts as soon as possible but no later than
the 8th of April.

Please visit the conference website for submission details and to view the conference program.
We have planned a superb scientific program and several workshops. Make sure you do not miss
this opportunity to learn about new microscopy methods.

http://www.brockhouse.mcmaster.ca/MSC-SMC2005/

Looking forward to your conference contributions.

Marcia Reid (on behalf of organizing committee)


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 18 16:49:04 2005



From: pwebster-at-hei.org (by way of MicroscopyListserver)
Date: Fri, 18 Mar 2005 16:59:16 -0600
Subject: [Microscopy] viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pwebster-at-hei.org) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, March 18, 2005 at 12:02:22
---------------------------------------------------------------------------

Email: pwebster-at-hei.org
Name: Paul Webster

Title-Subject: [Microscopy] [Filtered] Uranyl acetate problem

Question: To continue this interesting subject, which curiously relates to the subject Marc Pypaert brough up recently concerning uranyl acetate quality control:

When uranyl acetate was supplied in its undepleted form a saturated aqueous solution was around a 3% (w/v) concentration. More recently, in our lab we have only been able to get staining in some applications using a 5% solution. Now I read that 8% solutions are being recommended for usable staining.

I wonder what is being taken out of the uranyl acetate when it is being depleted?

On a related subject, the message I get from all the safety seminars I have to attend, is that the real poblem with uranyl acetate is not radioactivity but that it is toxic when ingested or inhaled. No amount of depleting will ever protect us from eating or inhaling the stuff.

Uranyl acetate disposal protocols take into account the toxic nature of the substance but becasue it is a natural isotope, there are no special protocols for disposing it as a radioactive substance.

Deep down I feel we were better off with the "old" radioactive uranyl acetate? Anyone know where I could buy a jar for myself? Maybe the "old" stuff is still being sold in less regulated parts of the world?

I welcome comments.

Paul Webster.



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 18 16:48:32 2005



From: david.audette-at-sylvania.com (by way of MicroscopyListserver)
Date: Fri, 18 Mar 2005 16:58:45 -0600
Subject: [Microscopy] viaWWW: ImageSlave board Manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (david.audette-at-sylvania.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, March 18, 2005 at 09:06:57
---------------------------------------------------------------------------

Email: david.audette-at-sylvania.com
Name: David Audette

Organization: OSRAM Sylvania

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello,

I seemed to have recently acquired the last ImageSlave board in the market and was not lucky enough to also get a manual with it. I do have a some setup pages but the directions refer to an elusive userís manual. If anyone can help me locate this manual, I would appreciate that. I am trying to capture images from an AMRAY 1845FE.

TIA

Dave Audette
OSRAM SYLVANIA
71 Cherry Hill Drive
Beverly, MA 01915
david.audette-at-sylvania.com


---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Sat Mar 19 14:37:26 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 19 Mar 2005 15:47:21 -0500
Subject: [Microscopy] Swift microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ritchie Sims wrote:
==============================================================
Does anyone know who, these days, supplies microscope stage point counter
attachments for petrographic use?

We have an old one made by Swift, of England, but my Google searching has
not turned up any current suppliers or manufacturers.
================================================================
There were two Swift microscope companies: one in the USA that is still
going and one in the Uk that was taken over by Prior Scientific some time
ago.

Most of the old Swift products are no longer available but you could try
contacting Prior to see of they can help.

E-mail: uksales-at-prior.com

Disclaimer: SPI Supplies has no commerical relationships with Swift or
Prior.

Chuck
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Sun Mar 20 05:20:56 2005



From: Barrie Wells :      Barrie.Wells-at-conwyvalley.com
Date: Sun, 20 Mar 2005 11:37:21 -0000
Subject: [Microscopy] Re: point Counter Supplier

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ritchie Sims enquired about a point counter to fit a petrographic microscope
(i.e. a rotating stage).

Charles Garber suggested trying Prior instruments (who purchased Swift and
hence took over production of the J-0415C electro-mechanical stage).

I think that my company (Conwy Valley Systems) makes the only device that
fits this description. If you know of any other, I would be interested to
hear of it.

When Prior withdrew from this market in 2002, my company faced a dilemma,
because we have a software product (www.petrog.com) which depends on
existence of a stepping stage. We tried to persuade Prior to continue
manufacture but they were effectively defeated by the high quality of their
product: it was so reliable, they rarely sold replacements, and the market
was not growing sufficiently to justify continuing manufacture.
We therefore commissioned the electronics department at our local university
to design an updated stepper, fully automated in both x and y, which we now
manufacture and sell (in very small quantities).
If you are interested, you can find out more at www.SteppingStage.com
We are sure that this is the only device of its kind in the world; we would
not have gone to all the trouble and expense of creating it if there had
been any alternative.
If you have any suggestions for new markets for this device, we would be
very pleased to hear from you (and I apologise if that is a commercial
plug).

Regards,

Barrie Wells
Conwy Valley Systems Limited



From MicroscopyL-request-at-ns.microscopy.com Sun Mar 20 20:00:54 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 20 Mar 2005 18:18:00 -0800
Subject: [Microscopy] Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul
According Merck Index, UA (chemical salt, not unknown stuff) is dissolvable
in the water up to 10% (I don't remember the temperature, but believe it's
RT). As I mentioned before, from my prospective UA is uranyl-acetate:
acetic salt of uranium. Every NORMAL chemical company will indicate the
quality of the product: analytical grade etc. It also normally indicates
how much impurities present. For instance, 98% pure (something like
laboratory grade). All EM suppliers just redistribute UA (correct me if I
am wrong) and somehow (magically) keep forgetting to indicate how pure
their material is. If you want "good stuff", you need order it
from chemical company, not re-distributor. Merck is a great company and
perhaps best for inorganic compounds (no interest but happy user for
decades when was in Europe). The problem, how you bring stuff in US? I
also think that every EM supplier should have responsibility for the
product quality. Regarding "depleted" - uranium is uranium and 0.7%
presence (or absence in depleted) of 235U does not matter. You also right
that radioactivity of UA in not an issue: Americans distributed 120 metric
tons of depleted uranium (yes) over Kosovo (Yugoslavia) grounds during the
invasion. Depleted uranium is a component of bullets and artillery
rounds. There is about few tons (if I remember correctly) of depleted
uranium in every Boeing plane (civilian). Uranium present danger as any
(and every) heavy metal. It's extremely unhealthy to breath UA
powder. Personally, I am using UA from Ted Pella without any problem (no
interest, happy user for many years). Last time I purpose a few years ago,
so things may changed. Normally, I am using 3% stock solution. If there
is a problem with dissolving occurred, one should test quality of water -
slightly basic water (an indication of bacterial contamination) will reduce
solubility of UA. Aqueous UA solutions are stable at pH 5-5.4. Sergey


At 04:59 PM 3/18/2005 -0600, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 10:10:14 2005



From: Richard Edelmann :      edelmare-at-MUOhio.edu
Date: Mon, 21 Mar 2005 11:21:57 -0500
Subject: [Microscopy] LR White Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

O.k., folks here is a weird one: I have a user who routinely uses LR
White, with good sucess, for emmbedding Arabidopsis (hypocotels).
However last week during an over night infiltration (as normal) 1-part ethanol
to 1-part LR White, the samples partially polymerized in the vials on a rotator.

Two questions:

(1) The LR White sample mix presently is a very viscous gel - i.e. not
completely polymerized. Any ideas for freeing/recovering the samples from
the gel? There are multiple samples in each vial and samples need to be flat
embedded for orientation.

(2) Any ideas what happened? I am leaning towards partially polymerized LR
White to start with - but maybe not. The LR White used was from a new bottle
well within expiration (Bad transportation or storage perhpas). However, the
LR White out of the bottle had normal viscosity (i.e. did not seem to be
partially polymerized before use). And how could it have polymerized 1:1 with
Ethanol, in sealed vials (tightly capped, wrapped in 3 layers of seal view & 3
layers of parafilm - perhaps overkill but it makes them happy i.e. the Ethanol
could not have evaporated).

The lab space is kept at 20-22C and certainly did not get over 30C. There
are no UV Lights in the hoods or room, and no windows (beside done over
night). No, likely polymerization source.


(No suppliers are being implicated here, and in fact it should be noted that
the supplier tech-support was very friendly to the user. We're just trying to
prevent a repeat, and having to start the samples all over again).


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 10:40:53 2005



From: Elaine Humphrey :      ech-at-interchange.ubc.ca
Date: Mon, 21 Mar 2005 08:53:27 -0800
Subject: [Microscopy] Re: Cost Recovery in Imaging Facilities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debby
As another response from Canada I would like to corroborate that what
you suggest is exactly right.

This is a facility where the only hard money coming in from the
University was my wages and everything else had to be cost recovery.
But a large number of the faculty are finding that their research
budgets are getting cut and are asking for help for the cost of
research internally. The University has responded by allocating some
indirect costs. It is still only soft money but it sure makes a
difference. It means that I can help keep the cost of research to the
individual PI at a reasonable level. Some of the faculty are also
helping write the federal grants for service contracts and personel.

We have done a good job in showing how easy it is to get a good TEM,
SEM and confocal image. Hence the graduate students own network plays
a part and they push to get the training. By making this facility
easy to use and welcoming, last year we had over 730 users from 258
account holders and 68 departments and groups. I have three full time
technicians now and a half time office person to help me and they are
all worth their weight in whatever metal is the most expensive at the
moment! But the key is the faculty. Without the need of the
research faculty there would be no need for this facility. As it is,
the need is still growing not diminishing around here. It is largely
up to the faculty to put the case to the administrators.
Administrators listen to faculty where they don't have to listen to
managers.

Elaine



} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 12:16:08 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 21 Mar 2005 12:28:37 -0600
Subject: [Microscopy] LR White Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Richard,

The exact same thing happened to me a couple years back, turning the LRW
into something resembling cottage cheese. Dr. Tom Phillips here at UM
correctly identified the source of the problem as too much residual
osmium in the tissue. We don't normally osmicate tissues destined for
immuno work, but a client had brought the tissue in already fixed, but
apparently not washed enough to get rid of all the excess osmium. The
sample was trashed. We never did figure out how to rescue it.

Hope this helps. If your tissue was not exposed to osmium, I'd love to
hear what the problem ended up being with it.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.edu]
Sent: Monday, March 21, 2005 10:22 AM
To: microscopy-at-MSA.Microscopy.com

O.k., folks here is a weird one: I have a user who routinely
uses LR White, with good sucess, for emmbedding Arabidopsis
(hypocotels).
However last week during an over night infiltration (as normal) 1-part
ethanol to 1-part LR White, the samples partially polymerized in the
vials on a rotator.

Two questions:

(1) The LR White sample mix presently is a very viscous gel - i.e. not
completely polymerized. Any ideas for freeing/recovering the samples
from the gel? There are multiple samples in each vial and samples need
to be flat embedded for orientation.

(2) Any ideas what happened? I am leaning towards partially polymerized
LR White to start with - but maybe not. The LR White used was from a
new bottle well within expiration (Bad transportation or storage
perhpas). However, the LR White out of the bottle had normal viscosity
(i.e. did not seem to be partially polymerized before use). And how
could it have polymerized 1:1 with Ethanol, in sealed vials (tightly
capped, wrapped in 3 layers of seal view & 3 layers of parafilm -
perhaps overkill but it makes them happy i.e. the Ethanol could not have
evaporated).

The lab space is kept at 20-22C and certainly did not get over
30C. There are no UV Lights in the hoods or room, and no windows
(beside done over night). No, likely polymerization source.


(No suppliers are being implicated here, and in fact it should
be noted that the supplier tech-support was very friendly to the user.
We're just trying to prevent a repeat, and having to start the samples
all over again).


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 14:18:15 2005



From: Yang, Ann-Fook :      YANGA-at-agr.gc.ca
Date: Mon, 21 Mar 2005 15:24:35 -0500
Subject: [Microscopy] LR White Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Richard,

I had similar experience. There was nothing wrong when I was doing immunogold fixation and embedding but it gave me results as you described when I was embedding osmium-fixed tissue. I had been unable to rescue any of those tissue stuck in gooey LRW.

It happened to osmium fixed tissue and older LR White. The shelf-life of LR White is not long if it is catalyzed by the manufacturer or vendor. Your new bottle might have been sitting in a cold room for a while and I bet you were embedding osmium-fixed tissue.

Nowadays, I purchase uncatalyzed LR White. I mix half a bottle with half of catalyst a day or two just before I need it and use a smaller bottle to keep the other half to minimize oxidation.
After mixing, I further divide them into aliquots so that I warm up small quantity each time. The results have been satisfactory.
I hope this helps.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.edu]
Sent: Monday, March 21, 2005 11:22 AM
To: microscopy-at-MSA.Microscopy.com

O.k., folks here is a weird one: I have a user who routinely uses LR
White, with good sucess, for emmbedding Arabidopsis (hypocotels).
However last week during an over night infiltration (as normal) 1-part ethanol
to 1-part LR White, the samples partially polymerized in the vials on a rotator.

Two questions:

(1) The LR White sample mix presently is a very viscous gel - i.e. not
completely polymerized. Any ideas for freeing/recovering the samples from
the gel? There are multiple samples in each vial and samples need to be flat
embedded for orientation.

(2) Any ideas what happened? I am leaning towards partially polymerized LR
White to start with - but maybe not. The LR White used was from a new bottle
well within expiration (Bad transportation or storage perhpas). However, the
LR White out of the bottle had normal viscosity (i.e. did not seem to be
partially polymerized before use). And how could it have polymerized 1:1 with
Ethanol, in sealed vials (tightly capped, wrapped in 3 layers of seal view & 3
layers of parafilm - perhaps overkill but it makes them happy i.e. the Ethanol
could not have evaporated).

The lab space is kept at 20-22C and certainly did not get over 30C. There
are no UV Lights in the hoods or room, and no windows (beside done over
night). No, likely polymerization source.


(No suppliers are being implicated here, and in fact it should be noted that
the supplier tech-support was very friendly to the user. We're just trying to
prevent a repeat, and having to start the samples all over again).


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 15:37:50 2005



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Mon, 21 Mar 2005 16:49:47 -0500
Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
} ----- Original Message -----
} From: "Ladd Research" {ladres-at-worldnet.att.net}
} To: "Microscopy Listserver" {Microscopy-at-microscopy.com}
} Cc: {john-at-microvisionlabs.com}
} Sent: Friday, February 25, 2005 4:44 PM
} Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed
}
}
} } The Bomar CPD was the forerunner of the Ladd CPD #28000 which was first
} } manufactured around 1970. The 1500 and the 28000 had a number of
} } similarities. There are still quite a few 28000s functioning so I'd
expect
} } the 1500 might be okay. They were well made. E-mail me your address and
} } we'll see if we have a manual for either CPD.
} }
} } John Arnott
} }
} } Ladd Research
} } 83 Holly Court
} } Williston, VT 05495
} }
} } On-line Catalog: http://www.laddresearch.com
} }
} } tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
} } fax: 1-802-660-8859
} } e-mail: ladres-at-att.net
} }
} }
}
} --------------------------------------------------------------------------
} --
} } --
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------------------
} --
} } ---
} }
} } Yes, someone just emailed me and said that The Bomar Company went under
} } about 20 years ago. I talked to Ernest Fullem Co. and they said they
} } had also carried the Bomar CPDs back in the day but never had any at
} } their location. They would just have them sent to the customer when one
} } was ordered. So they have no manuals or such. That is likely the same
} } with Ladd as well.
} } Looks like there are a few manuals out there from the emails I have been
} } getting and I should be ok on that front. Thanks for everyone's
} } responses. You have been very helpful to me.
} }
} } John Knowles
} } MicroVision Labs, Inc.
} }
} }
} } -----Original Message-----
} } } From: john hoffpauir [mailto:hoffpajo-at-yahoo.com]
} } Sent: Thursday, February 24, 2005 5:55 PM
} } To: John Knowles; Microscopy-at-microscopy.com
} } Subject: [Microscopy] Re: SEM: Critical-Point Dryer Info. needed
} }
} }
} }
} } ------------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ------------------------------------------------------------------------
} } -------
} }
} } i have found through google a refence that used the
} } bomar cpd. ladd sold it years back.
} } john
} } --- John Knowles {john-at-microvisionlabs.com} wrote:
} }
} } }
} } }
} } }
} } ------------------------------------------------------------------------
} } ------
} } } The Microscopy ListServer -- Sponsor: The
} } } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } }

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: ladres-at-att.net





From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 15:48:04 2005



From: Ian Hallett :      IHallett-at-hortresearch.co.nz
Date: Tue, 22 Mar 2005 10:02:43 +1200
Subject: [Microscopy] Re: LR White Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard

This issue has cropped up on occasion in the past. Premature polymerisation is not necessarily related to osmication of the tissue but is often related to older resin or resin that has had poor storage, a frequent problem was overheating during transport and also to some components in the tissue or extraneous media. Like many people we have for many years bought the uncatalysed resin and made up a bottle only when we need it. We have had no problem in the 10 or so years we have done this. I dredged
through the archives for a comment I made a number of years ago (1997 to be exact) and append a quote from Roy Gillett of London Resin.

"The reason this pre-polymerisation occurs only with tissue must be
something to do with a tissue constituent catalysing polymerisation.
Older resin is much more susceptible to this than fresh monomer because
of the significant polymer growth that will inevitably have occurred
in the monomer. The most likely 'endogenous catalyst' from
previous experience is likely to be an amine or peroxide moiety in
the tissue"

Ian

Ian Hallett
HortResearch
Mt Albert Research Centre, Private Bag 92 169
Auckland, New Zealand
Fax +64 9 815 4201
Telephone +64 9 815 4200
EMail ihallett-at-hortresearch.co.nz


} } } "Richard Edelmann" {edelmare-at-MUOhio.edu} 22/03/2005 4:21:57 a.m. } } }


------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

O.k., folks here is a weird one: I have a user who routinely uses LR
White, with good sucess, for emmbedding Arabidopsis (hypocotels).
However last week during an over night infiltration (as normal) 1-part ethanol
to 1-part LR White, the samples partially polymerized in the vials on a rotator.

Two questions:

(1) The LR White sample mix presently is a very viscous gel - i.e. not
completely polymerized. Any ideas for freeing/recovering the samples from
the gel? There are multiple samples in each vial and samples need to be flat
embedded for orientation.

(2) Any ideas what happened? I am leaning towards partially polymerized LR
White to start with - but maybe not. The LR White used was from a new bottle
well within expiration (Bad transportation or storage perhpas). However, the
LR White out of the bottle had normal viscosity (i.e. did not seem to be
partially polymerized before use). And how could it have polymerized 1:1 with
Ethanol, in sealed vials (tightly capped, wrapped in 3 layers of seal view & 3
layers of parafilm - perhaps overkill but it makes them happy i.e. the Ethanol
could not have evaporated).

The lab space is kept at 20-22C and certainly did not get over 30C. There
are no UV Lights in the hoods or room, and no windows (beside done over
night). No, likely polymerization source.


(No suppliers are being implicated here, and in fact it should be noted that
the supplier tech-support was very friendly to the user. We're just trying to
prevent a repeat, and having to start the samples all over again).


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


______________________________________________________

The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 17:26:02 2005



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Mon, 21 Mar 2005 15:38:57 -0800
Subject: [Microscopy] Re: LR White Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Richard,

Having used LRW from over 20 years, I have found there are two conditions,
which result in a partially polymerized gel: 1) older LWR; 2) older LRW
mixture 1:1 with EtOH creates enough heat to cause premature polymerization.

I have taken similar tissue as yours, simply cut away as much of the gel
material as is convenient, placed in 100% LRW in the refrigerator overnight
as an additional precaution to thoroughly infiltrate the gel + tissue with
the new LRW: then the next day, embed as usual and polymerize.

I have actually kept a bottle of older LWR medium grade around in the
refrigerator so I can utilize this odd behavior of partially polymerization.
In the case of small specimens for which I want a vertical orientation in
the gel capsules, I use the following: simple place the specimen in gel
capsule, allow partial polymerization at room temperature, removed and trim
'block' in the correct orientation, re-infiltrate with fresh LRW, embed and
polymerize in the oven. May not be conventional, but it has been a great
solution of specimens like nematodes.

As Ann-Fook Yang suggested, purchase uncatalyzed LR White. I mix it up all
at once and kept it in the refrigerator. I also refrigerate my EtOH series.

Regards,
Ken
_______________________________________
Kenneth L. Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N Willamette Blvd.
Portland, OR 97203 USA

Tel.: 503.493.8861



On 3/21/05 8:21 AM, "Richard Edelmann" {edelmare-at-MUOhio.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} O.k., folks here is a weird one: I have a user who routinely uses LR
} White, with good sucess, for emmbedding Arabidopsis (hypocotels).
} However last week during an over night infiltration (as normal) 1-part ethanol
} to 1-part LR White, the samples partially polymerized in the vials on a
} rotator.
}
} Two questions:
}
} (1) The LR White sample mix presently is a very viscous gel - i.e. not
} completely polymerized. Any ideas for freeing/recovering the samples from
} the gel? There are multiple samples in each vial and samples need to be flat
} embedded for orientation.
}
} (2) Any ideas what happened? I am leaning towards partially polymerized LR
} White to start with - but maybe not. The LR White used was from a new bottle
} well within expiration (Bad transportation or storage perhpas). However, the
} LR White out of the bottle had normal viscosity (i.e. did not seem to be
} partially polymerized before use). And how could it have polymerized 1:1 with
} Ethanol, in sealed vials (tightly capped, wrapped in 3 layers of seal view & 3
} layers of parafilm - perhaps overkill but it makes them happy i.e. the Ethanol
} could not have evaporated).
}
} The lab space is kept at 20-22C and certainly did not get over 30C. There
} are no UV Lights in the hoods or room, and no windows (beside done over
} night). No, likely polymerization source.
}
}
} (No suppliers are being implicated here, and in fact it should be noted that
} the supplier tech-support was very friendly to the user. We're just trying
} to
} prevent a repeat, and having to start the samples all over again).
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 350 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 18:10:30 2005



From: moss-at-relia.net
Date: Mon, 21 Mar 2005 17:22:52 -0700 (MST)
Subject: [Microscopy] Re: LR White Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} I have had this problem in the past. In my case it was the
microcetrfuge tubes that we used. The colored tubes would do this
consistantly, whereas the clear (not white) were fine. Something in the
coloring agent was acting to catalyse the LR White.

Bill McManus
Mt Ogden Scientific Services
moss-at-relia.net
801/334-6677
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} O.k., folks here is a weird one: I have a user who routinely uses LR
} White, with good sucess, for emmbedding Arabidopsis (hypocotels).
} However last week during an over night infiltration (as normal) 1-part
} ethanol
} to 1-part LR White, the samples partially polymerized in the vials on a
} rotator.
}
} Two questions:
}
} (1) The LR White sample mix presently is a very viscous gel - i.e. not
} completely polymerized. Any ideas for freeing/recovering the samples
} from
} the gel? There are multiple samples in each vial and samples need to be
} flat
} embedded for orientation.
}
} (2) Any ideas what happened? I am leaning towards partially polymerized
} LR
} White to start with - but maybe not. The LR White used was from a new
} bottle
} well within expiration (Bad transportation or storage perhpas). However,
} the
} LR White out of the bottle had normal viscosity (i.e. did not seem to be
} partially polymerized before use). And how could it have polymerized 1:1
} with
} Ethanol, in sealed vials (tightly capped, wrapped in 3 layers of seal view
} & 3
} layers of parafilm - perhaps overkill but it makes them happy i.e. the
} Ethanol
} could not have evaporated).
}
} The lab space is kept at 20-22C and certainly did not get over 30C.
} There
} are no UV Lights in the hoods or room, and no windows (beside done over
} night). No, likely polymerization source.
}
}
} (No suppliers are being implicated here, and in fact it should be noted
} that
} the supplier tech-support was very friendly to the user. We're just
} trying to
} prevent a repeat, and having to start the samples all over again).
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 350 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 19:11:19 2005



From: Beaurega :      beaurega-at-westol.com
Date: Mon, 21 Mar 2005 20:24:05 -0500
Subject: [Microscopy] Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Paul,

I was a wet analytical research chemist for many years before being called
back into microscopy to restart a 'mothballed' TEM lab. Now it's gone and
I was forced to retire early. Anyway, I agree with all the comments of
Sergey.

A rose is a rose is a rose. Depleted uranium is something like 8% U235 and
depleted it is 4-6% U235. It is still quite radioactive. In either case,
the radioactivity doesn't change the chemical reactions of DU.
If you hold your 'pancake' geiger counter or equivalent near DU, it will
not make single clicks like background cosmic rays do. It will emit a
continuous tone on the times one scale even through a glass bottle and its
surrounding metal can. I had access to pounds of the stuff gathered over
the years by a chemist that I worked with in that wet lab. The gamma
radiation was detectable 12 feet away through walls and locked metal
cabinets. So inhalling DU or UA is bad.

Unfortunately, depleted uranium carries with it the implication it is 100%
depleted. It is only 40-60% depleted.
For example, a depleted silver mine seems to indicate no silver is left in
the mine.

If there is a microscopy problem with UA being used as a chemical reagent,
then it is with the purity, contamination, assay, or some other similar
problem.

A possible solution:
It is well known that before atomic absorption (atomic spectroscopy) that
the determinations of sodium were done using uranyl acetate as a raw
material. The UA was converted to zinc UA. The sodium was determined
gravimetrically in a platinum dish as the sodium salt. So, you might find
some 'old' UA available in a lab where it is almost impossible to dispose
of any unused UA cheaply today. These AA or wet labs might have pound or a
quarter-pound bottle of UA 'stored' somewhere in their labs. The
manufacturers / sellers, as I recall, were Fisher Chemical, B&A, and
Mallinkrodt.

Walk through a wet lab and see if your geiger counter goes off. There is
no need to open cabinets or metal containers. If you get a hit, the
original container should be a thin-walled (rusted?) steel capped and taped
can with a shrink wrapped plastic seal on the DUA bottle cap.

Good hunting (I hope),

Paul

Sergy,

There is a lot of unused older DUA in the US. It's not all on tanks and
ammo as U°. I found out the hard way that it is a almost impossible to
cheaply dispose of or sell UNUSED UA. Legal issues came up, shipping
issues were raised, people ran for cover, and risks over transportation
accidents were raised. I was told nobody, not even the US government,
wanted the stuff. I feel this 'old sodium UA reagent' could and should be
used in small quantities for research or by hospitals to help people like
Paul.


------------------------
At 04:59 PM 3/18/05 -0600, pwebster-at-hei.org wrote:
}
}
} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 20:02:45 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 21 Mar 2005 18:30:34 -0800
Subject: [Microscopy] Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 18, 2005, at 2:59 PM, by way of MicroscopyListserver wrote:

} I wonder what is being taken out of the uranyl acetate when it is
} being depleted?
}
} On a related subject, the message I get from all the safety seminars I
} have to attend, is that the real poblem with uranyl acetate is not
} radioactivity but that it is toxic when ingested or inhaled. No amount
} of depleting will ever protect us from eating or inhaling the stuff.
}
} Uranyl acetate disposal protocols take into account the toxic nature
} of the substance but becasue it is a natural isotope, there are no
} special protocols for disposing it as a radioactive substance.
}
Dear Paul,
The fissionable isotope U-235 is removed from depleted U, and, since
this is commonly done by gas centrifugation of UF6, it is hard for me
to see how the act of depletion could change the staining properties of
UAc. My best guess is that there is still some UFx and UFxO mixed in
with the UO2, but, since I don't know how the UF6 is treated after the
centrifugation process, this is strictly a wild guess. It is certainly
true that U-238, being an alpha-particle emitter, poses no threat from
the radiation unless it is inhaled or ingested. I have not looked into
the chemical toxicity, but I expect it is not good for you, as are a
lot of heavy metals. I know that Ra and, especially, Pu are toxic. An
inhaled U-containing particle that is retained in the lung can emit
alpha-particles that will be close enough to a living cell to cause a
lot of ionization within that cell, so inhaling a particle in the
sub-um size range will potentially expose a cell to a massive amount of
damage and can lead to cancer, and the same applies to an ingested
particle if the U finds its way into the tissue (rather than simply
being eliminated). Finally, whether there are or are not special
protocols for disposing of U depends on the state you are living in.
Some states require very expensive procedures for disposal, and in
others one can simply wash U down the sink with large amounts of water,
so everyone should check with the appropriate safety office (there
could also be applicable local ordinances).
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 21:18:40 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 21 Mar 2005 19:46:30 -0800
Subject: [Microscopy] Re: Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 21, 2005, at 5:24 PM, Beaurega wrote:

} If you hold your 'pancake' geiger counter or equivalent near DU, it
} will
} not make single clicks like background cosmic rays do. It will emit a
} continuous tone on the times one scale even through a glass bottle and
} its
} surrounding metal can. I had access to pounds of the stuff gathered
} over
} the years by a chemist that I worked with in that wet lab. The gamma
} radiation was detectable 12 feet away through walls and locked metal
} cabinets. So inhalling DU or UA is bad.
}
Dear Beauriga,
What one reads from UAc at the distances you mention is, indeed, gamma
radiation, which is emitted from some of the isotopes in the U decay
chains. There will be a (nearly) steady state established where each
isotope in each decay chain (different for U-238 and U-235) has the
appropriate number of atoms such that the activities of each isotope
are the same; i.e., for each isotope, one atom is produced by the
parent isotope(s) for every one that decays. It is the gammas that can
cause damage at long distances, but each alpha which enters a
cell--thus acting over distances shorter that its very short range,
less than the thickness of the dead layer of the skin--does much more
damage that the gammas.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 21 23:29:05 2005



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Tue, 22 Mar 2005 07:46:44 +0200
Subject: [Microscopy] Re: LR White Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Richard

We had a similar problem.
We went the other way.
Opened another bottle of LR white and ditch that bottle of ethanol to general Lab use. The samples were lost. Forced it to polymerise but they went brittle. For us the amount of LR white in the bottle left and a unhappy user the cost of a smiley user was worth more. The EMU staff did the next run as a service (but also to verify that the user did it all correct.)
Best of luck ;)

We purchase only unanalysed LR White and we store all our LR white in the fridge. The lab does get occasionally very hot when the air-conditioning fails. Above 30 degree is not uncommon. On the batch that did not polymerise we had a few concerns:

1) The catalyser reacted with the packaging and had a brownish colour, but 90% of the bottle was used without problems.

2) The bottle might have got a bit old, 6 months after the catalyser were added.

3) The 99.999% ethanol might have absorbed to much water from the atmosphere.

We have resulted in Ethanol from glass containers only. Never let a bottle get past 6 months and never use a catalyst if it looks suspicious. The problem did not reoccur yet. Touch wood, ouch...



Since some mail do get Lost, Bounces, etc Please send a duplicate/copy of all urgent mail to:

coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gaborone
Botswana
Phone : +267 355 2462/5222
Mobile : +267 718 36547
Fax : +267 318 5097
e-mail : coetzees-at-mopipi.ub.bw

-----Original Message-----
} From: moss-at-relia.net [mailto:moss-at-relia.net]
Sent: Tuesday, March 22, 2005 2:23 AM
To: edelmare-at-MUOhio.edu
Cc: microscopy-at-msa.microscopy.com

} I have had this problem in the past. In my case it was the
microcetrfuge tubes that we used. The colored tubes would do this
consistantly, whereas the clear (not white) were fine. Something in the
coloring agent was acting to catalyse the LR White.

Bill McManus
Mt Ogden Scientific Services
moss-at-relia.net
801/334-6677
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} O.k., folks here is a weird one: I have a user who routinely uses LR
} White, with good sucess, for emmbedding Arabidopsis (hypocotels).
} However last week during an over night infiltration (as normal) 1-part
} ethanol
} to 1-part LR White, the samples partially polymerized in the vials on a
} rotator.
}
} Two questions:
}
} (1) The LR White sample mix presently is a very viscous gel - i.e. not
} completely polymerized. Any ideas for freeing/recovering the samples
} from
} the gel? There are multiple samples in each vial and samples need to be
} flat
} embedded for orientation.
}
} (2) Any ideas what happened? I am leaning towards partially polymerized
} LR
} White to start with - but maybe not. The LR White used was from a new
} bottle
} well within expiration (Bad transportation or storage perhpas). However,
} the
} LR White out of the bottle had normal viscosity (i.e. did not seem to be
} partially polymerized before use). And how could it have polymerized 1:1
} with
} Ethanol, in sealed vials (tightly capped, wrapped in 3 layers of seal view
} & 3
} layers of parafilm - perhaps overkill but it makes them happy i.e. the
} Ethanol
} could not have evaporated).
}
} The lab space is kept at 20-22C and certainly did not get over 30C.
} There
} are no UV Lights in the hoods or room, and no windows (beside done over
} night). No, likely polymerization source.
}
}
} (No suppliers are being implicated here, and in fact it should be noted
} that
} the supplier tech-support was very friendly to the user. We're just
} trying to
} prevent a repeat, and having to start the samples all over again).
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 350 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}





From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 06:07:55 2005



From: j.bilde-at-risoe.dk
Date: Tue, 22 Mar 2005 13:20:09 +0100
Subject: [Microscopy] viaWWW: dislocation loop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Mike,

If you know what a dislocation loop is, you will probably also know the definition of the Burgers vector of a dislocation. A prismatic dislocation loop is a loop whose Burgers vector does not lie in the plane of the loop.

A dislocation loop whose Burgers vector does lie in the plane of the loop can glide in this plane. A prismatic loop, on the other hand, can only move in the plane of the loop by climb. It may, however, be able to glide along a prism defined by the Burgers vector and the line vectors of the loop.

Best regards,
Jorgen.

{:::} --- {:::} --- {:::} --- {:::} --- {:::} --- {:::}

Joergen B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 4677 5802 (direct)
phone: +45 4677 4677 (switchboard)
fax: +45 4677 5758
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm



-----Original Message-----
} From: by way of MicroscopyListserver [mailto:mikeraj-at-streamyx.com]
Sent: 15. marts 2005 04:16
To: microscopy-at-ns.microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mikeraj-at-streamyx.com) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Sunday, March 13, 2005 at 11:48:59
---------------------------------------------------------------------------

Email: mikeraj-at-streamyx.com
Name: Mike Marks

Organization: Cornell University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi, I understand what a dislocation loop is, but what is a
prismatic dislocation loop ? Thanks !

---------------------------------------------------------------------------




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 08:07:46 2005



From: dmphase-at-udc.ernet.in (by way of MicroscopyListserver)
Date: Tue, 22 Mar 2005 08:20:43 -0600
Subject: [Microscopy] viaWWW: Jeol JSM 5600 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dmphase-at-udc.ernet.in) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, March 21, 2005 at 22:41:06
---------------------------------------------------------------------------

Email: dmphase-at-udc.ernet.in
Name: D.M.Phase

Organization: Inter University Consortium, India

Title-Subject: [Microscopy] [Filtered] Jeol JSM 5600 SEM

Question: Whether anybody knows website on which service mannual for above SEM machine is available online?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 09:48:42 2005



From: Frank Eggert :      eggert-at-mikroanalytik.de
Date: Tue, 22 Mar 2005 17:04:16 +0100
Subject: [Microscopy] X-ray lines and data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For all who have interest in EDX and X-ray data:

The new version of MA-Table software (X-ray line table, spectra
simulation, calculation of overlap situations and detection limits in
EPMA, ...) is ready for WEB-download. A display of of mass absorption
coefficient curves for any element and a calculation of absorption for
given energy and material thicknesses are new.

See the other new features:
http://microanalyst.net/manual.html#link3
Go to download:
http://microanalyst.net/registr.phtml

Best regards

Frank Eggert

======================
www.microanalyst.net
======================



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 11:18:43 2005



From: Richard =?iso-8859-1?Q?L=E9veill=E9?= :      leveille.richard-at-uqam.ca
Date: Tue, 22 Mar 2005 12:23:25 -0500
Subject: [Microscopy] VP-SEM/ESEM (non-cryo) observations of ice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear microscopists,
We would like to be able to observe minerals and microorganisms in situ in
natural ice samples (permafrost, ground ice, sea ice) without destroying,
drying or modifying the sample.
We have an Hitachi FE-VP-SEM equipped with a Peltier-type cold stage (-30
deg C) and have tried looking at a few samples without much success in
observing micron-sized bacteria either using the ESED detector or
backscattered e-detector. We can however make general observations of the
ice over varying periods of time depending on sample type and size, as well
as observing conditions (temp, pressure, AV...).

All the literature I have found on ice observations is done with cryo-SEM
at much lower temperatures. Could this be done with a variable pressure
SEM or ESEM with a cold stage? Obviously sublimation is an issue and will
likely negatively impact the imaging capabilities, but can we expect a good
enough signal to see bacterial cells (roughly 1-2 microns)?
Any ideas or suggestions would be appreciated.
Cheers,
-Richard

Richard Léveillé
Postdoctoral Fellow-Stagiaire Postdoctoral
Centre Geotop-UQAM-McGill
Université du Québec à Montréal
C.P. 8888, Succ. Centre-Ville
Montréal, QC H3C 3P8
514-987-3000 5662# ***New/Nouveau***
514-987-4080
514-987-3635 (fax)
{http://www.geotop.uqam.ca/index.php?option=content&task=view&id=86&Itemid=79} W {http://www.geotop.uqam.ca/index.php?option=content&task=view&id=86&Itemid=79} ebpage





From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 13:56:06 2005



From: Lehman, Ann R :      Ann.Lehman-at-trincoll.edu
Date: Tue, 22 Mar 2005 15:10:16 -0500
Subject: [Microscopy] old uranyl acetate available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

I have some old U.A. from Fisher (#U-4) and Baker (#4192) free to a good
home, if I can figure out the correct way to ship it. Ideally the taker
would be someone local, and can come pick it up.

I also have uranyl nitrate (Fisher U-7, Baker #4196, and Mallinckrodt
#8640), as well as zinc uranyl acetate (Fisher #U-11). Also some thorium
compounds. Most of these reagents are in unopened brown bottles
apparently kept in the dark (in tins, etc). I would dearly love to
unload it all!

I acquired this material from a local lab that was shutting down, and I
carried various boxes and gadgets and some very nice pieces back to my
lab without realizing how much UA (and variants) was in there.

If anyone is interested, just let me know.

cheers,
Ann

Ann Lehman
Assistant Director
Electron Microscopy Facility
Mailstop: LSC314
Trinity College
300 Summit Street
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 15:06:56 2005



From: Salamon, Daniel :      Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Tue, 22 Mar 2005 16:20:00 -0500
Subject: [Microscopy] Sputtering system usage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Our facility recently bought a Gatan PECS 682 Ion Beam Sputtering system. Currently, we are using it for high-resolution coating of samples for our field-emission SEM. It is not currently under heavy use, but accounting keeps asking me to come up with a cost structure for next year.

Can you offer any information on how you set up your charging scheme (included in SEM, per sample, per unit time, per target material)?

Also, I would really appreciate it if anybody can give me an idea about how long different targets last before needing replacement. I understand this depends on system usage, but any information would help. Are there any other operating expenses?


Thanks,

Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-081 ECERF Bldg
9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 17:46:54 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Tue, 22 Mar 2005 15:58:25 -0800
Subject: [Microscopy] Re: Re: LR White Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We had the problem of LR White partial polymerization only when there
was EtOH in the mixture. In our case, the fixative wasn't an issue. We
never had it with our researchers but started to see the problem with
students. More than 20 yrs ago, we started with using an infiltration
series with EtOH like one does with Epoxy and propylene oxide i.e. (2
parts EtOH:1 part LRW; 1part EtOH:1 part LRW; 1 part EtOH: 2 parts LRW).
This was followed by 2 changes in pure LRW. The researchers didn't have
the problem, but a few of the students did. I came to believe that it
was because some of the EtOH was left in the mixture i.e. the students
weren't as careful as the researchers. At that point, we stopped using
an infiltration series and went from 100% EtOH to 3 changes in pure
LRW. We never saw the problem again.

Partially polymerized blocks:
We tried desperately to save a couple of the partially polymerized
blocks. First of all, they NEVER polymerized even after 2 yrs in the
oven. We did however try to reclaim some of them. We cut the tissues
out of the partially polymerized blocks and put them first in propylene
oxide and let them soak and then in epoxy. We did this with vacuum
infiltration of the epoxy. We could cut them, but they weren't the
best. These were hard to get samples however so we felt lucky to get
anything.

Good Luck
Judy


Judy Murphy, PhD
Microscopy, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net

moss-at-relia.net wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 17:49:38 2005



From: tttan-at-simtech.a-star.edu.sg (by way of MicroscopyListserver)
Date: Tue, 22 Mar 2005 18:02:41 -0600
Subject: [Microscopy] viaWWW: High voltage feedthrough for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tttan-at-simtech.a-star.edu.sg) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, March 22, 2005 at 10:42:51
---------------------------------------------------------------------------

Email: tttan-at-simtech.a-star.edu.sg
Name: TT Tan

Organization: SIMTech

Title-Subject: [Microscopy] [Filtered] High voltage feedthrough for TEM

Question: Hello all. May seems a little strange but i would like to know who are the manufacturers that produce such high voltage feedthrough for TEM. I would like to get one for experimental purposes. Thanks in advance

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 18:57:47 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 22 Mar 2005 17:11:48 -0800
Subject: [Microscopy] Re: Re: Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Geiger counter normally detects only beta-emission. Alpha-emission may not
be detected other than by scintillation counter. Gamma-irradiation (and
some beta) in uranium is mostly due the quick slowing down the
beta-particles in the material, I forgot German word for that. It's also
called the "secondary" radiation. Uranium itself do not emit gamma. There
is some accumulation of U byproduct as correctly mentioned Bill Tivol,
which may emit alpha/beta or gamma particles (if gamma-photon is considered
to be a "particle"). From the "radiation" point of view - both 235U and
238U are both radioactive (no gamma). Alpha particles could travel just a
part of millimeter in the air. Piece of paper will stop most of them. So,
they are not dangerous to human being unless somehow you get them inside
your body. Beta particles also do not travel much - 1/2 in of plexiglass
(of glass bottle) effectively cut them out. Gamma is naturally present
everywhere: X-ray, travel by airplane, CAT scan (a lot!) etc. Amount of
gamma emitted by U is just negligible in compare how much charcoal power
plant emits into the atmosphere every second. If you happens to live in
the luxurious house with granite facade (even counter top on the kitchen
will count) - you will have noticeable higher radioactive background
around. Granite happens to release tiny amount of radon-gas, which is
highly radioactive and really dangerous. Some particular fillers for
concrete do the same. So, gamma is everywhere. In laboratory practice most
common and dangerous is beta-emission. You need to understand that
exposure-dose rapidly decreased with the distance (by power of two), so you
need to keep space between you and radioactive source: tweezers, glovers
etc. Water effectively adsorbs beta/alpha particles, so aqueous solutions
are less dangerous than solids .... and so on. If one wants to see how
"dangerous" the common laboratory environment is - take the jar with KCl
from your laboratory shelf, pour about 50 grams of KCl into Petri Dish and
measure radioactivity by Geiger counter... you will detect noticeable
radioactivity of some natural potassium isotopes... By the way, to measure
gamma - you need to use special badges from Radiation Safety Office or
special attachments to your Geiger counter (you will know if you have it,
because it' a few $K). Normal/standard Geiger counter DO NOT detect
gamma! Sergey

At 07:46 PM 3/21/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Tue Mar 22 19:28:27 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Wed, 23 Mar 2005 07:18:42 -0330
Subject: [Microscopy] choosing a digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How high are you talking about for the voltage?
And what is it for/from?

cheers

rtch


Date sent: Tue, 22 Mar 2005 18:02:41 -0600
To: microscopy-at-microscopy.com
} From: tttan-at-simtech.a-star.edu.sg (by way of MicroscopyListserver)

Hello Ritchie,

I am looking at 120kV. Basically its for feeding High Voltage into high
vacuum environment.

Regards
Thiam Teck
Research Engineer
Precision Measurement Group
SIMTech


-----Original Message-----
} From: Ritchie Sims [mailto:r.sims-at-auckland.ac.nz]
Sent: Wednesday, 23 March, 2005 9:41 AM
To: Tan Thiam Teck, Dr; microscopy-at-microscopy.com

It would appear that modern digital cameras dedicated to photomicroscopy
have balanced dynamic observation with pixel count. However, our
applications for a new optical workstations do not anticipate dynamic
observation (e.r., moving critters). Have I missed a camera manufacturer
that does offer a high resolution model, which does not provide a high frame
rate? For example, for real time observation and digital display, I'd
anticipate we'd only require 8fps (approximately 1024x768)... but would like
to capture imagery at high resolution, e.g., 5Mp.

Testamonials and sales representatives should contact me off list ...

tia & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 05:05:28 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Wed, 23 Mar 2005 07:48:32 -0330
Subject: [Microscopy] SEM: EMF radiation from mass spectrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to best predict how a mass spectrometer will affect an SEM,
both of which to be installed in the near future. The MS manufacturer
responds with several measurements near the front of the instrument and
magnet that are near 0.1 - 0.5 milliTestlas (measured at ~10cm) ... and that
around the sides and rear, that measuremnts fall off to earth's natural
levels( ~0.05mT). The space for the SEM, 15M distant, will be evaluated for
EMF at approximately 0.5milliGauss (10,000mT = 1mG), however at specific
frequencies.

Should I not be concerned with the emission from MS instrument because it is
static?

tia & cheerios ... shAf (a MS newbie) :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 07:13:01 2005



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 23 Mar 2005 14:21:39 +0100
Subject: [Microscopy] Re: viaWWW: High voltage feedthrough for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I don't know if TEM manufacturer buy they insulator by these companies,
but here are a few in that domain which have some choice. You can contact
too some vaccum component manufacturer like Huntington, Thermo-VG or KJ
Lesker. They sell such feedthrough.

The following are companies which manufacter themself.

In north america, and more or less elsewere
Ceramaseal, a company from Ceram Tech
ISI, distributed by MDC vacuum components

In europe
Frialit (formerly Degussa)
Desmarquet


Hope it helps

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Tue, 22 Mar 2005, by way of MicroscopyListserver wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tttan-at-simtech.a-star.edu.sg) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, March 22, 2005 at 10:42:51
} ---------------------------------------------------------------------------
}
} Email: tttan-at-simtech.a-star.edu.sg
} Name: TT Tan
}
} Organization: SIMTech
}
} Title-Subject: [Microscopy] [Filtered] High voltage feedthrough for TEM
}
} Question: Hello all. May seems a little strange but i would like to know who are the manufacturers that produce such high voltage feedthrough for TEM. I would like to get one for experimental purposes. Thanks in advance
}
} ---------------------------------------------------------------------------
}




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 07:31:20 2005



From: Peter Van Osta :      pvosta-at-maia-scientific.com
Date: Wed, 23 Mar 2005 14:39:11 +0100
Subject: [Microscopy] Re: choosing a digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

When choosing a camera there are two main basic principles to take into
account. The first is the spatial dimensions of the chip and the second
is its dynamic range.

The size of the chip and the size of its pixels will determine its
spatial qualities (simplified). The larger the chip size the larger its
field of view can be. The smaller the individual pixel size the better
its spatial resolution at a given resolution of the objective
(http://ourworld.compuserve.com/homepages/pvosta/pcrnyq.htm). With small
pixel sizes you do not need to add intermediate magnification steps to
capture the full resolution of the objective.

However as a general rule of thumb, the dynamic range of the camera
decreases with decreasing pixel size. Cooling a CCD wil decrease the
contribution of its own "thermal noise" and increases its usefull
dynaimc range.

The more pixels the more data you need to transfer to a PC. For consumer
grade cameras, USB and even FireWire will do. For more bandwidth a
CameraLink camera is better, but as USB and FireWire now come with every
computer this is of course easier.

Regards,

Peter

----------------------------------------------
Peter Van Osta, MD

Director Imaging
MAIA SCIENTIFIC
Cipalstraat 3
B-2440 Geel, Belgium
Tel.: +32 (0)14 570 620
Mobile: +32 (0)497 228 725
Fax.: +32 (0)14 570 621
Email: pvosta-at-maia-scientific.com
Website: www.maia-scientific.com
A Harvard Bioscience Company
----------------------------------------------

============================================================
michael shaffer wrote:
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} It would appear that modern digital cameras dedicated to photomicroscopy
} have balanced dynamic observation with pixel count. However, our
} applications for a new optical workstations do not anticipate dynamic
} observation (e.r., moving critters). Have I missed a camera manufacturer
} that does offer a high resolution model, which does not provide a high frame
} rate? For example, for real time observation and digital display, I'd
} anticipate we'd only require 8fps (approximately 1024x768)... but would like
} to capture imagery at high resolution, e.g., 5Mp.
}
} Testamonials and sales representatives should contact me off list ...
}
} tia & cheerios ... shAf :o)
} Avalon Peninsula, Newfoundland
} www.micro-investigations.com (in progress)




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 07:51:53 2005



From: Karl Hagglund :      hagglundk1-at-nku.edu
Date: Wed, 23 Mar 2005 09:05:06 -0500
Subject: [Microscopy] Thanks S-570 maintenance questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all on the list who responded to my questions about the S-570
objective aperture. I received answers and the correct illustration for
my manual within an hour or so of posting my note, and I am thankful for
each and every response.

Hitachi's service people were also in touch with me shortly after my
posting and I would like to especially thank them for helping me out
with a 20 year old instrument that is no longer under service contract.
Their knowledge and experience with the instrument helped us to
troubleshoot the ultimate source of the problem and obtain the proper
part numbers to fix it.


Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 08:54:39 2005



From: Will Zhou/FTTCSF :      WillZ-at-ftpc.fpcusa.com
Date: Wed, 23 Mar 2005 09:04:32 -0600
Subject: [Microscopy] Light Microscope -- Leitz Laborlux 12...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All:

We just donated a light microscope (Leitz Laborlux 12 Pol S) to a local high school, and I helped setting it up. The teacher is quite happy about it. However, unfortunately we can't locate the manual and she would like to have one. A Google search on Leitz microscope did not turn up manufacturer's website. I wonder if anybody can help us to locate the manufacturer or find a manual of Leitz Laborlux 12.

Cheers,


Will Zhou

Formosa Plastics
201 Formosa Drive
Point Comfort, TX 77978

Phone: 361-987-8341
Fax: 361-987-7487



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 10:30:38 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Wed, 23 Mar 2005 09:41:51 -0700
Subject: [Microscopy] choosing a digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Michael,

your observation is correct in the sense that many camera manufacturers have
placed a high priority on "live" imaging, i.e., imaging with a frame rate
that allows to change microscope parameters in real time (focus, position,
etc.). This is very important if the parameters need to be changed while
observing on the computer screen. My own experience is that this becomes
almost impossible if the frame rate drops below something like 10 fps. It
just becomes very frustrating because you have to change parameters too
slowly.
On the other hand, there is of course a drive to more and more pixels.
Regardless of how big the pixels are or how big the chip is, the more pixels
you have, the more information you have to transfer to the computer, and the
slower the frame rate gets.
Now, to answer your question: There are a number of cameras out there with 5
MPixel or more. **Commercial on** For example, our ColorView III camera. **
Commercial off* The amount of data that we collect allows us to reach a
frame rate of about 2-3 fps at this resolution. To get a higher frame rate,
the camera can be binned (i.e., we reduce the resolution by combining 4 or 8
pixels into one before transmitting the image) or a sub-area can be read
out. That way we can trade off resolution for speed. So you could use this
camera at 5 MPixel for the type of imaging you describe, or use one of the
other modes to get a higher frame rate (in this case about 8 fps with a 2x
binning). What I am describing is valid for many cameras, not only ours, by
the way.
Another possibility to play this game is to use a "pixel-shift" camera.
These camera usually have a smaller chip (for example 1300x1024) attached to
a piezo. They then take consecutive images while slightly (fractions of a
pixel) moving the chip. The multiple images are then assembled into a
resulting image with larger resolution. Obviously, this will not really
allow you live imaging in this mode, as you have to take consecutive images
during which the object cannot move. You can check out the Olympus DP-70 as
an example, but there are many of those cameras on the market.
Contact me off-line if you want more information.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: michael shaffer [mailto:michael-at-shaffer.net]
Sent: Wednesday, March 23, 2005 03:49
To: MSA listserver

It would appear that modern digital cameras dedicated to photomicroscopy
have balanced dynamic observation with pixel count. However, our
applications for a new optical workstations do not anticipate dynamic
observation (e.r., moving critters). Have I missed a camera manufacturer
that does offer a high resolution model, which does not provide a high frame
rate? For example, for real time observation and digital display, I'd
anticipate we'd only require 8fps (approximately 1024x768)... but would like
to capture imagery at high resolution, e.g., 5Mp.

Testamonials and sales representatives should contact me off list ...

tia & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 12:15:38 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Wed, 23 Mar 2005 10:28:08 -0800 (PST)
Subject: [Microscopy] Re: Re: Re: Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i never knew my granite counter tops were dangerous
other than when i hit my head on them.
--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Geiger counter normally detects only beta-emission.
} Alpha-emission may not
} be detected other than by scintillation counter.
} Gamma-irradiation (and
} some beta) in uranium is mostly due the quick
} slowing down the
} beta-particles in the material, I forgot German word
} for that. It's also
} called the "secondary" radiation. Uranium itself do
} not emit gamma. There
} is some accumulation of U byproduct as correctly
} mentioned Bill Tivol,
} which may emit alpha/beta or gamma particles (if
} gamma-photon is considered
} to be a "particle"). From the "radiation" point of
} view - both 235U and
} 238U are both radioactive (no gamma). Alpha
} particles could travel just a
} part of millimeter in the air. Piece of paper will
} stop most of them. So,
} they are not dangerous to human being unless somehow
} you get them inside
} your body. Beta particles also do not travel much -
} 1/2 in of plexiglass
} (of glass bottle) effectively cut them out. Gamma is
} naturally present
} everywhere: X-ray, travel by airplane, CAT scan (a
} lot!) etc. Amount of
} gamma emitted by U is just negligible in compare how
} much charcoal power
} plant emits into the atmosphere every second. If
} you happens to live in
} the luxurious house with granite facade (even
} counter top on the kitchen
} will count) - you will have noticeable higher
} radioactive background
} around. Granite happens to release tiny amount of
} radon-gas, which is
} highly radioactive and really dangerous. Some
} particular fillers for
} concrete do the same. So, gamma is everywhere. In
} laboratory practice most
} common and dangerous is beta-emission. You need to
} understand that
} exposure-dose rapidly decreased with the distance
} (by power of two), so you
} need to keep space between you and radioactive
} source: tweezers, glovers
} etc. Water effectively adsorbs beta/alpha particles,
} so aqueous solutions
} are less dangerous than solids .... and so on. If
} one wants to see how
} "dangerous" the common laboratory environment is -
} take the jar with KCl
} from your laboratory shelf, pour about 50 grams of
} KCl into Petri Dish and
} measure radioactivity by Geiger counter... you will
} detect noticeable
} radioactivity of some natural potassium isotopes...
} By the way, to measure
} gamma - you need to use special badges from
} Radiation Safety Office or
} special attachments to your Geiger counter (you will
} know if you have it,
} because it' a few $K). Normal/standard Geiger
} counter DO NOT detect
} gamma! Sergey
}
} At 07:46 PM 3/21/2005, you wrote:
}
}
}
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} -------------------------------------------------------------------------------
} }
} }
} } On Mar 21, 2005, at 5:24 PM, Beaurega wrote:
} }
} } } If you hold your 'pancake' geiger counter or
} equivalent near DU, it will
} } } not make single clicks like background cosmic rays
} do. It will emit a
} } } continuous tone on the times one scale even
} through a glass bottle and its
} } } surrounding metal can. I had access to pounds of
} the stuff gathered over
} } } the years by a chemist that I worked with in that
} wet lab. The gamma
} } } radiation was detectable 12 feet away through
} walls and locked metal
} } } cabinets. So inhalling DU or UA is bad.
} } Dear Beauriga,
} } What one reads from UAc at the distances
} you mention is, indeed,
} } gamma radiation, which is emitted from some of the
} isotopes in the U
} } decay chains. There will be a (nearly) steady
} state established where
} } each isotope in each decay chain (different for
} U-238 and U-235) has the
} } appropriate number of atoms such that the
} activities of each isotope are
} } the same; i.e., for each isotope, one atom is
} produced by the parent
} } isotope(s) for every one that decays. It is the
} gammas that can cause
} } damage at long distances, but each alpha which
} enters a cell--thus acting
} } over distances shorter that its very short range,
} less than the thickness
} } of the dead layer of the skin--does much more
} damage that the gammas.
} } Yours,
} } Bill Tivol, PhD
} } EM Scientist and Manager
} } Cryo-Electron Microscopy Facility
} } Broad Center, Mail Code 114-96
} } California Institute of Technology
} } Pasadena CA 91125
} } (626) 395-8833
} } tivol-at-caltech.edu
} }
} }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} 10833 Le Conte Ave, Room 33-080
} Los Angeles, CA 90095
}
} Phone: (310) 825-1144 (office)
} (310) 206-1029 (Lab)
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}
}




__________________________________
Do you Yahoo!?
Yahoo! Small Business - Try our new resources site!
http://smallbusiness.yahoo.com/resources/


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 13:21:23 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 23 Mar 2005 11:49:11 -0800
Subject: [Microscopy] Re: SEM: EMF radiation from mass spectrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 23, 2005, at 3:18 AM, michael shaffer wrote:

} I would like to best predict how a mass spectrometer will affect an
} SEM,
} both of which to be installed in the near future. The MS manufacturer
} responds with several measurements near the front of the instrument and
} magnet that are near 0.1 - 0.5 milliTestlas (measured at ~10cm) ...
} and that
} around the sides and rear, that measuremnts fall off to earth's natural
} levels( ~0.05mT). The space for the SEM, 15M distant, will be
} evaluated for
} EMF at approximately 0.5milliGauss (10,000mT = 1mG), however at
} specific
} frequencies.
}
} Should I not be concerned with the emission from MS instrument because
} it is
} static?
}
Dear ShAf,
0.1 milliTesla at 10 cm will be an unmeasurable field (unless there
are some ultra-sensitive detectors such as SQUIDs) at 15 m. The fields
fall off with a power of more than two. The transformers from the SEM
power supply are likely to be sources of larger fields than the MS.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 13:23:39 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 23 Mar 2005 11:51:28 -0800
Subject: [Microscopy] Re: Re: Re: Re: Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 23, 2005, at 10:28 AM, john hoffpauir wrote:

} i never knew my granite counter tops were dangerous
} other than when i hit my head on them.
}
Dear John,
Only if you eat them.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 14:23:37 2005



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 23 Mar 2005 14:36:33 -0600
Subject: [Microscopy] Texas Society for Microscopy 40th Anniversary Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Pre-registration deadline has been extended until Tuesday March 29 for
the Texas Society for Microscopy (TSM) Spring Meeting April 14 - 16, in Las
Colinas Texas. The deadline for the Photo Contest has been extended for the
same time. Maps and directions to the hotel and the workshop are also out
on the web site

The Spring 2005 40th Anniversary meeting of the Texas Society for
Microscopy (TSM) is quickly approaching. The meeting will be held April 14
- 16, 2005 in Las Colinas Texas. You can go to the TSM website for program
and registration information, web address provided below.

TSM web site http://www.texasmicroscopy.org/

Early registration is strongly encouraged in order for the society to have
an accurate head count for the meeting. This will allow better utilization
of TSM funds.

This will be our big 40th anniversary meeting. We will be providing
parallel sessions for biological sciences and material sciences. This
meeting offers an excellent opportunity to network, talk with vendors about
the latest technology and obtain information about the latest hot topics in
your field. There is information valuable to everyone. There will also be
special sessions held on Friday afternoon April 15 celebrating the history
of the TSM.



--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 16:03:45 2005



From: Tamara Howard :      thoward-at-unm.edu
Date: Wed, 23 Mar 2005 15:16:53 -0700 (MST)
Subject: [Microscopy] Hitachi H-7500 TEM question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all - we're just curious: Who else out there in EM-land has this
microscope?

Thanks!

Tamara

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 17:45:02 2005



From: hastingscl-at-kidneybx.com (by way of MicroscopyListserver)
Date: Wed, 23 Mar 2005 17:58:20 -0600
Subject: [Microscopy] viaWWW: Codonics 1660 dye sub printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (hastingscl-at-kidneybx.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, March 23, 2005 at 15:33:09
---------------------------------------------------------------------------

Email: hastingscl-at-kidneybx.com
Name: Cindy Hastings Smith

Organization: Nephropath

Title-Subject: [Microscopy] [Filtered] MListserver:Codonics 1660 dye sub printer

Question: Greetings to all--
We have a Codonics NP 1660 due sublimation printer available for sale. It has had limited usage and now that we rarely print images--we were hoping to find it a new home. Please contact me off-line if interested.

Thanks
Cindy Hastings Smith
Nephropath
10810 Executive Center Drive
Danville Building Suite 100
Little Rock, AR 72211
501-604-2695
hastingscl-at-kidneybx.com

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 23 17:45:57 2005



From: dg38-at-drexel.edu (by way of MicroscopyListserver)
Date: Wed, 23 Mar 2005 17:59:03 -0600
Subject: [Microscopy] viaWWW: lift out station for FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dg38-at-drexel.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, March 23, 2005 at 15:14:14
---------------------------------------------------------------------------

Email: dg38-at-drexel.edu
Name: Daibin Ge

Organization: Drexel Univ.

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi, all:

I am interesting in purchasing an ex-situ lift out station for FIB prepared TEM samples. Could you please provide some information about the possible vendors?

thanks a lot.

Daibin

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 24 00:49:59 2005



From: moss-at-relia.net
Date: Thu, 24 Mar 2005 00:02:18 -0700 (MST)
Subject: [Microscopy] Re: viaWWW: Codonics 1660 dye sub printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} What are you asking for the printer?

Bill McManus
Mt Ogden Scientific Services
www.mtogdensci.com
moss-at-relia.net
801/334-6677
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (hastingscl-at-kidneybx.com) from
} http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
} Wednesday, March 23, 2005 at 15:33:09
} ---------------------------------------------------------------------------
}
} Email: hastingscl-at-kidneybx.com
} Name: Cindy Hastings Smith
}
} Organization: Nephropath
}
} Title-Subject: [Microscopy] [Filtered] MListserver:Codonics 1660 dye sub
} printer
}
} Question: Greetings to all--
} We have a Codonics NP 1660 due sublimation printer available for sale.
} It has had limited usage and now that we rarely print images--we were
} hoping to find it a new home. Please contact me off-line if interested.
}
} Thanks
} Cindy Hastings Smith
} Nephropath
} 10810 Executive Center Drive
} Danville Building Suite 100
} Little Rock, AR 72211
} 501-604-2695
} hastingscl-at-kidneybx.com
}
} ---------------------------------------------------------------------------
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 24 02:23:44 2005



From: gillian.2.brown-at-gsk.com
Date: Thu, 24 Mar 2005 08:35:21 +0000
Subject: [Microscopy] Re: Hitachi H-7500 TEM question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tamara,
I have one here at GSK, R&D at Stevenage, UK (first one in the UK) and
when colleagues at our Toxicology site at Ware, UK needed a new one in
2002 they replaced a Phillips CM10 with the H7500.
I am very happy with ours and have not plate developed since it's
acquisition in 2000.

Regards

Gillian Brown

Histopathology Group
Asthma and Allergy Disease Biology
ri- CEDD.
GlaxoSmithKline Medicines Research Centre,
Gunnelswood Road,
STEVENAGE,
Hertfordshire.
SG1 2NY
tel. +44 (0)1438 764119
fax. +44 (0)1438 764782
email. gillian.2.brown-at-gsk.com





"Tamara Howard" {thoward-at-unm.edu}
23-Mar-2005 22:16

To
"Microscopy Server" {microscopy-at-microscopy.com}
cc

Subject
Hitachi H-7500 TEM question








------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi, all - we're just curious: Who else out there in EM-land has this
microscope?

Thanks!

Tamara

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|







From MicroscopyL-request-at-ns.microscopy.com Thu Mar 24 04:38:23 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Thu, 24 Mar 2005 07:20:45 -0330
Subject: [Microscopy] RE: SEM: EMF radiation from mass spectrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bill Tivol writes ...

} 0.1 milliTesla at 10 cm will be an unmeasurable field (unless there
} are some ultra-sensitive detectors such as SQUIDs) at 15 m. The fields
} fall off with a power of more than two. The transformers from the SEM
} power supply are likely to be sources of larger fields than the MS.

I am hoping my misleading mT/mG conversion did not affect your answer.
That is, I stated 10,000milliTeslas = 1milliGauss. The opposite is actually
true, i.e., 1mT = 10,000 mG.

cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 24 06:13:47 2005



From: mdelann1-at-jhmi.edu (by way of Ask-A-Microscopist)
Date: Thu, 24 Mar 2005 06:26:50 -0600
Subject: [Microscopy] AskAMicroscopist: Does malachite green aggregate lipids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mdelann1-at-jhmi.edu) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, March 23, 2005 at 14:20:37
---------------------------------------------------------------------------

Email: mdelann1-at-jhmi.edu
Name: Michael Delannoy

Organization: Johns Hopkins School of Medicine

Education: Graduate College

Location: Baltimore, MD

Question: Does malachite green aggregate lipids from membranes
if used in combo with GA as a primary fix.
If we see large lipid bodies can this be a post
fix artifact (ie does it pool lipid from membranes
into a lipid body)?

thanks
mike d

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 24 09:15:45 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Thu, 24 Mar 2005 10:29:48 -0800
Subject: [Microscopy] Re: AskAMicroscopist: Does malachite green aggregate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A lot of work on malachite green + glutaraldehyde fixative for lipids
was done by Teichman et al. in the 1970s. I don't remember the specifics
but they did do some chromatography to back up the results they saw in
the EM.

Geoff

by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From MicroscopyL-request-at-ns.microscopy.com Thu Mar 24 09:29:33 2005



From: tiaross-at-comcast.net (by way of Ask-A-Microscopist)
Date: Thu, 24 Mar 2005 09:42:49 -0600
Subject: [Microscopy] AskAMicroscopist: paint analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tiaross-at-comcast.net) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, March 24, 2005 at 07:53:42
---------------------------------------------------------------------------

Email: tiaross-at-comcast.net
Name: Tia Ross

Organization: Savannah College of Art & Design

Education: Graduate College

Location: Savannah, GA

Question: I'm conducting paint analysis on a mid-19th century building for my MFA thesis, and need to test paint samples for white lead. Is there a chemical spot test I can do on cross-sections cast in resin?

Thank you!

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 24 18:31:41 2005



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Thu, 24 Mar 2005 21:28:13 -0500
Subject: [Microscopy] Self Upgrading an XL30

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On the macro scale, application of a dilute sodium sulfide (Na2S) solution makes lead-
based white paint turn black.

I don't know if it would work on mounted cross-sections, but worth a try.

cheers

rtch


Date sent: Thu, 24 Mar 2005 09:42:49 -0600
To: microscopy-at-microscopy.com
} From: tiaross-at-comcast.net (by way of Ask-A-Microscopist)

} OK, our XL30 FEG is a workhorse, but the computer is now so old and
} slow it seems to take a week to do anything.
} I have heard that people have done their own upgrades to these
} computers and was wondering if anyone would share their experiences
} (off line and if I get enough me-toos I'll summarize to the list). I
} would be very grateful.
} We have the NT based standalone system with a PII and 64 Meg of RAM
} with a Coreco MX card.
} I was thinking of building a new computer with my spare MX card but if
} anyone has an in house conversion to an FX card I'd love to hear about
} it.


John Mansfield PhD CPhys CSci MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143 USA
Phone: (734) 936-3352
FAX (734) 763-2282
Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"
AIM: thejfmjfm
Yahoo: thejfmjfm
Skype: thejfmjfm, (preferred)




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 24 22:00:48 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Thu, 24 Mar 2005 22:11:39 -0600
Subject: [Microscopy] Re: Re: AskAMicroscopist: paint analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Tia

Just a reminder that the best way to look at paint is in darkfield. You
will remove obscuring glare and get much better color and edge discrimination.

Hope this is helpful,

Best regards,
Barbara Foster
Microscopy/Microscopy Education

We've moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website
to learn more about "Optimizing LIght Microscopy". Copies still available
through MME... even for class-room lots ... and we give quantity discounts.


At 06:44 PM 3/24/2005, Ritchie Sims wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 14:42:45 2005



From: john hoffpauir :      hoffpajo-at-yahoo.com
Date: Fri, 25 Mar 2005 12:55:10 -0800 (PST)
Subject: [Microscopy] Re: Re: Re: Re: Re: Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

well i have never tried to eat it have eaten off it.
my teeth wouldn't stand up to it. lol
--- Bill Tivol {tivol-at-caltech.edu} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
}
} On Mar 23, 2005, at 10:28 AM, john hoffpauir wrote:
}
} } i never knew my granite counter tops were
} dangerous
} } other than when i hit my head on them.
} }
} Dear John,
} Only if you eat them.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}
}



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From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 16:09:47 2005



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 25 Mar 2005 16:22:26 -0600
Subject: [Microscopy] printing directory list from CD ROM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have a large number of CD ROMS and need to generate a printout of
the files on the disks.

How can we do this on a Mac running OSX 10.3?

Thank you very much.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 16:34:38 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 25 Mar 2005 16:47:34 -0600
Subject: [Microscopy] Sputter coating question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are doing LOTS of sputter coating of various metals these days for a
group of electrical engineering department clients. Up until recently
the platinum and titanium coatings that have been applied have been
shiny and mirror-like, as one would expect. Just recently, however, the
target has been showing a decidedly scaled, rough surface and the
coating on the silicon substrate shows microcracks. We are using a
target that is 0.5mm (0.02") thick, which is thicker than the normal
target for this machine, but it worked fine when installed.

The coater is being run very hard----90mA for repeated runs of 4 minutes
each, often many times a day. It is Peltier-cooled, but the target area
and photoresist on the sample is heating up, although the heat build-up
has not been measured. The coater is turbo-pumped.

My questions are: what could be causing the scaling on the target itself
and the microcracks on the surface being coated, and will a sputter
coating designed for general EM lab use stand up to this kind of hard
use over time?

Thanks for any thoughts.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 17:56:43 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 25 Mar 2005 16:09:19 -0800
Subject: [Microscopy] Re: Sputter coating question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm using a Denton Desk II coater with Au/Pd or Pt.
This is for coating semiconductors and other specimens.
I coat at 20mA for between 30 seconds and 50 seconds
depending on intended SEM KV. 90mA seems rather high
to me. Poor vacuum (} 125mTorr) and high current will
produce bad coating. It is best, IMO to go for longer
time at low current and have a good vacuum (85-95mTorr).

This works in my SEM from 100V to 8KV at 200pA probe
current or less without problem.

I don't see why the coater needs to run for 4 minutes.
What kind is it.

gary g.


At 02:47 PM 3/25/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 19:15:59 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 25 Mar 2005 17:28:32 -0800
Subject: [Microscopy] EDAX Cryospec problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Are others having problems with the EDAX Cryospec
LN2-free detector unit? Mine is continually having
problems of refrigerant noise affecting high mag
imaging. Additionally, the MMR compressor cooling
unit flakes out and raises detector temperature such
that HV ON is OK but all peaks are warped out. As
a result, the system is unusable. This is very bad.
A lot of work is being deferred because of this.

LN2-free was/is a very desirable option. However, it
appears to be unreal. The EDAX Genesis app is very
nice. Thus, I am forced to deal with a standard
LN2 detector. This is a huge pain, as you all know.

Are there others out there with similar experiences?
Any solutions?

Are there other suppliers of LN2-free detectors that
actually work well by themselves and do not negatively
affect SEM imaging? Please let me know.

gary g.



From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 19:21:56 2005



From: mtabanpour-at-wisc.edu (by way of MicroscopyListserver)
Date: Fri, 25 Mar 2005 19:34:58 -0600
Subject: [Microscopy] AskAMicroscopist: microscopy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mtabanpour-at-wisc.edu) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Friday, March 25, 2005 at 11:47:49
---------------------------------------------------------------------------

Email: mtabanpour-at-wisc.edu
Name: Menachem Tabanpour

Organization: University of Wisconsin-Madison

Education: Undergraduate College

Location: Madison, WI, USA

Question: I am currently working on a project studying sands under a
binocular microscope and I am having trouble mounting the sands on
slides; my poblem is that the epoxy I am using has too many bubbles
in it and it's too defficults to handle. Can you please suggest what
I can use instead and/or how I can mount slides without bubbles in
between the particles?

Thanks,

MT

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 20:34:37 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 25 Mar 2005 21:47:37 -0500
Subject: [Microscopy] Mounting sand grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

MT wrote:
=====================================================
Question: I am currently working on a project studying sands under a
binocular microscope and I am having trouble mounting the sands on slides;
my poblem is that the epoxy I am using has too many bubbles in it and it's
too defficults to handle. Can you please suggest what I can use instead
and/or how I can mount slides without bubbles in between the particles?
=====================================================
The Tacky Dot® Slides manufactured by SPI Supplies might be just what you
need. I would suggest the 300 um "dots" for sand, assuming it is of the
size of typical "beach" sand. See URL
http://www.2spi.com/catalog/new/tacky.shtml

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 20:38:15 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 25 Mar 2005 21:51:14 -0500
Subject: [Microscopy] Imaging Quantum dots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I would appreciate tips on imaging 2-5 nm quantum dots as we have not done
this in the past. We will do the imaging using a Philips CM-100 TEM
configured for high resolution. I am assuming that a carbon film support
grid would be preferable but what about contrasting?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy





From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 20:45:16 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Fri, 25 Mar 2005 21:57:29 -0500
Subject: [Microscopy] Re: printing directory list from CD ROM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

We normally just do a screen capture of the opened CD-R (shift + apple
+ 4. Draw box with the curser that appears and it will appear as a picture
on the desktop.
Print this out and tape a copy to the disk case.

If you need to generate a list in WORD than you can transfer the picture
into OCR software and process.

I would appreciate hearing if you find a simpler way.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



On 3/25/05 5:22 PM, "John J. Bozzola" {bozzola-at-siu.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} We have a large number of CD ROMS and need to generate a printout of
} the files on the disks.
}
} How can we do this on a Mac running OSX 10.3?
}
} Thank you very much.




From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 20:58:10 2005



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 25 Mar 2005 19:13:52 -0800
Subject: [Microscopy] Re: AskAMicroscopist: microscopy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (mtabanpour-at-wisc.edu) from
} http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
} on Friday, March 25, 2005 at 11:47:49
} ---------------------------------------------------------------------------
}
} Email: mtabanpour-at-wisc.edu
} Name: Menachem Tabanpour
}
} Organization: University of Wisconsin-Madison
} Education: Undergraduate College
} Location: Madison, WI, USA
}
} Question: I am currently working on a project studying sands under a
} binocular microscope and I am having trouble mounting the sands on
} slides; my poblem is that the epoxy I am using has too many bubbles
} in it and it's too defficults to handle. Can you please suggest what
} I can use instead and/or how I can mount slides without bubbles in
} between the particles?
}
} MT

This may sound silly, but it works - it's a Project MICRO approach to
sand. Use a hole punch to make a hole in a piece of card. Cover the
hole with a piece of clear (not "Magic") tape. Scatter the sand on
the tape. The "slides" are quite durable.
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/


From MicroscopyL-request-at-ns.microscopy.com Fri Mar 25 23:32:03 2005



From: Tay Yee Yan :      one_twinklestar-at-yahoo.com.sg
Date: Sat, 26 Mar 2005 13:44:04 +0800 (CST)
Subject: [Microscopy] Re: Re: Sputter coating question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gary:

How do you decide which current of the sputter is
needed for certain SEM KeV? Does it apply the same to
FESEM? Pardon me for my ignorant because I just
started to use SEM.

Another question is, some recommend to use carbon as
coating but recently when i used it as a coating, i
found that my sample is coated with "furry stuff"
which I believe it should be due to carbon, under
FESEM. If I really need to use carbon coating, what
can I do to reduce such "furry stuff"?

Thanks a lot!

Regards,
Yee Yan
Nanyang Technological University
--- Gary Gaugler {gary-at-gaugler.com} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} I'm using a Denton Desk II coater with Au/Pd or Pt.
} This is for coating semiconductors and other
} specimens.
} I coat at 20mA for between 30 seconds and 50 seconds
} depending on intended SEM KV. 90mA seems rather
} high
} to me. Poor vacuum (} 125mTorr) and high current
} will
} produce bad coating. It is best, IMO to go for
} longer
} time at low current and have a good vacuum
} (85-95mTorr).
}
} This works in my SEM from 100V to 8KV at 200pA probe
} current or less without problem.
}
} I don't see why the coater needs to run for 4
} minutes.
} What kind is it.
}
} gary g.
}
}
} At 02:47 PM 3/25/2005, you wrote:
}
}
}
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} -------------------------------------------------------------------------------
} }
} } We are doing LOTS of sputter coating of various
} metals these days for a
} } group of electrical engineering department clients.
} Up until recently
} } the platinum and titanium coatings that have been
} applied have been
} } shiny and mirror-like, as one would expect. Just
} recently, however, the
} } target has been showing a decidedly scaled, rough
} surface and the
} } coating on the silicon substrate shows microcracks.
} We are using a
} } target that is 0.5mm (0.02") thick, which is
} thicker than the normal
} } target for this machine, but it worked fine when
} installed.
} }
} } The coater is being run very hard----90mA for
} repeated runs of 4 minutes
} } each, often many times a day. It is
} Peltier-cooled, but the target area
} } and photoresist on the sample is heating up,
} although the heat build-up
} } has not been measured. The coater is turbo-pumped.
} }
} } My questions are: what could be causing the scaling
} on the target itself
} } and the microcracks on the surface being coated,
} and will a sputter
} } coating designed for general EM lab use stand up to
} this kind of hard
} } use over time?
} }
} } Thanks for any thoughts.
} }
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility---We Do Small
} Well!
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-5414
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} }
} }
} }
} }
}
}
}

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Log on to Messenger with your mobile phone!
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From MicroscopyL-request-at-ns.microscopy.com Sat Mar 26 02:02:13 2005



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Sat, 26 Mar 2005 10:19:45 +0200
Subject: [Microscopy] RE: Mounting sand grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Charles.
We have done some work lately that did require some work on san grains as well. For stereo viewing followed by EM investigation the grains we placed carefully with Teflon coated forceps on an SPI conductive sticky tab on a Aluminium pin type stub. We did one slide for transmitted light microscope viewing and that was done on a cavity slide embedded in standard Araldite mixture. Worked well. If bubbles is a problem (assuming the grains are porous?) Follow the vacuum infiltration for biological samples. Done that on a geological Calcite sample. All voids were infiltrated.
Hope this helps

Here is some references of the work done:


· "Geomorphological and geochemical evidence for the palaeo-environmental evolution of the northern Makgadikgadi sub-basin, Botswana.", Susan Ringrosea,, Philippa Huntsman-Mapilaa, Ali Basira Kampunzub, William Downeyc, Stephan H Coetzeec, Bernard Vinkb, W. Mathesond, C. Vanderposte., Palaeogeography, Palaeloclimatology, Palaeoecology. 217/3-4 pp 265-287

· "Degradation of linear dunes in north west Ngamiland, Botswana and the implications for luminescence dating of periods of aridity" M.J. McFarlane, F.D. Eckardt, S. Ringrose, S.H. Coetzee and J.R. Kuhn Quaternary International, in press

· "The origin of the 'basal sandstone' of the Kalahari Sediments in North West Ngamiland, Botswana - implications regarding identification of the Kalahari Sediments"".,McFarlane, M.J., Coetzee, S.H., (submitted Palaeogeography, Palaeoclimatology, Palaeoecology)

} -----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
} Sent: Saturday, March 26, 2005 4:48 AM
} To: MICROSCOPY BB
} Subject: [Microscopy] Mounting sand grains
}
}
}
}
} --------------------------------------------------------------
} ----------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} -----------------
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} MT wrote:
} =====================================================
} Question: I am currently working on a project studying sands under a
} binocular microscope and I am having trouble mounting the
} sands on slides;
} my poblem is that the epoxy I am using has too many bubbles
} in it and it's
} too defficults to handle. Can you please suggest what I can
} use instead
} and/or how I can mount slides without bubbles in between the
} particles?
} =====================================================
} The Tacky Dot® Slides manufactured by SPI Supplies might be
} just what you
} need. I would suggest the 300 um "dots" for sand, assuming
} it is of the
} size of typical "beach" sand. See URL
} http://www.2spi.com/catalog/new/tacky.shtml
}
} Chuck
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
} ==================================================
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Sat Mar 26 16:17:07 2005



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 26 Mar 2005 14:33:36 -0800
Subject: [Microscopy] Project MICRO news

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Project MICRO is MSA's outreach program; you can read about it at the
URL below. Nestor has just posted several revisions of the MICRO web
page. There are a few new books and websites, new links on the
classroom activities page, and a new microscope design in the "Buying
school microscopes" section.

A MICRO informational brochure is now available. It's designed to
recruit both teachers and microscopist-volunteers. Want copies?
I've printed 500. I'll be traveling in April, so get your request to
me next week, or wait till May.

I've been trying to send this information to MSA's local societies,
but the listing on the MSA website is outdated; I haven't managed to
reach these: Alabama, Connecticut, Delta College, Metropolitan, and
Philadelphia. If you're a member of one of those groups, will you
please forward this Email to a society officer?

Caroline

--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/


From MicroscopyL-request-at-ns.microscopy.com Sun Mar 27 21:57:29 2005



From: Barbara :      bfoster-at-mme1.com
Date: Sun, 27 Mar 2005 21:57:10 -0600
Subject: [Microscopy] Re: AskAMicroscopist: microscopy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear MT,

Have you tried double-sided tape? It should do a good job for you.

Good hunting!
Barbara Foster
Microscopy/Microscopy Education

We've moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website
to learn more about "Optimizing LIght Microscopy". Copies still available
through MME... even for class-room lots ... and we give quantity discounts.

;

At 07:34 PM 3/25/2005, mtabanpour-at-wisc.edu wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 00:36:03 2005



From: Alon Sabban :      alon-at-quantomix.com
Date: Mon, 28 Mar 2005 08:51:08 +0200
Subject: [Microscopy] WETSEM workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear members,

We would like to inform you of a joint workshop of JEOL and QuantomiX
introducing the WETSEM technology.

The workshop will take place on the 4th of May, 2005, at JEOL's U.S.
headquarters at Peabody, MA. We will cover Life and Material science
applications with presentations and live demonstrations. Attendees are
encouraged to send their samples.

For more details and to register, please refer to :

http://www.jeol.com/sem/docs/Quantomix%20Workshop%201.pdf

or contact:

Ms. Donna Guarrera

Phone: 978 536 2224
email: guarrera-at-jeol.com

Mr. Alon Sabban
Phone: 617 522 1789
Email: alon-at-quantomix.com





From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 06:57:44 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Mon, 28 Mar 2005 09:11:21 -0500
Subject: [Microscopy] Fwd: DNA Structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy

Do you have an application for a sputter coater or a laboratory heater?

I am sure you know that most SEM sputter coaters are made to run in the 5 to
30mA range for normal coating, but super fine grain the Chromium coaters may
use in excess of 100mA. The crunch is that the latter technique only
requires coating for just a few seconds.

In the development of sputter coaters many of us played around with trying
to reduce the heating effect of the system on the specimen; we melted some
plastics when trying too hard! In the time periods and currents that you
that you are using there must be a good deal of heat being placed on the
specimen and target heating must also be taking place. Thus transformations
related to specimen and to target should not be unexpected. The thicker
target, provided it is making good contact, should not made any difference.

Sounds as if you are having fun, hope the above eases your mind?

Regards

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
www.emcourses.com tel +44 1280 816512 fax +44 1280 814007

----- Original Message -----
} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
To: {microscopy-at-microscopy.com}
Sent: Friday, March 25, 2005 11:47 PM

Hi

The main reasons for changing sputter coating parameters will relate to the
specimen form and to the resolution required.

Should the specimen surface be extremely complex (hills valleys and holes)
multiple coats, and even changing the angle of the specimen in relation to
the target, may be the only methods to ensure a good coating for say 15kV.
However if you are coating to obtain the highest resolution images greater
care must be taken no to overcoat and multiple coatings should not be used.
Remember multiple coating does make the metal structure more pronounced!

Some of our ideas on coating will be found under Hints and Tips on
www.emcourses.com.

Hope this helps?

Regards

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
www.emcourses.com tel +44 1280 816512 fax +44 1280 814007

----- Original Message -----
} From: "Tay Yee Yan" {one_twinklestar-at-yahoo.com.sg}
To: "Gary Gaugler" {gary-at-gaugler.com} ; "Tindall, Randy D."
{TindallR-at-missouri.edu}
Cc: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com}
Sent: Saturday, March 26, 2005 6:44 AM


} Date: Thu, 24 Mar 2005 09:26:32 -0500
} To: micro
} From: Greg Erdos {gwe-at-.ufl.edu}
} Subject: [Microscopy] DNA Structure
}
} Dear Colleagues,
} I have a user who wants to do the following. I would appreciate
} comments on AFM vs Low angle rotary shadowing}
}
}
} She'll be separating genomic DNA prepared under conditions that retain 3-D
} structures, such as hairpins, Holliday junctions, etc. by a 2-D gel
} approach. A multigene family we're studying is organized as
} quasipalindromic pairs with overlapping 5' UTRs. We want to ask whether
} the 3-D structure of the actively transcribed gene pair differs from that
} of transcriptionally silent gene pairs. We hypothesize that the
} intergenic regions of silent pairs are often in cruciform-like structures,
} making them good targets to donate sequences during gene conversion of the
} actively transcribed pair. She's been making artificial constructs that
} can reasonably be expected to form cruciforms, to act as controls. We'd
} like to substantiate this by EM as well as the 2-D gel system, then apply
} the same procedures to genomic fragments if we can feasibly isolate some
} to look at. It seems to me that low-angle rotary shadowing would yield
} comparable results to AFM for this application,
}
} Gregory W. Erdos Ph.D.
} Assistant Director, Biotechnology Program
} Scientific Director, Electron Microscopy
} P.O. Box 118525
} 217 Carr Hall
} University of Florida
} Gainesville, FL 32611
} gwe-at-ufl.edu
} 352-392-1295
} fax- 352-846-0251

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 08:19:54 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 28 Mar 2005 08:32:36 -0600
Subject: [Microscopy] Re: Sputter Coating Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

Many thanks for the replies to my original question on the scaling Pt
target and microcracks in the specimen coating. The consensus seems to
be that overheating is the problem.

I wasn't clear in my original posting about why we are doing this.
These specimens are not being coated for SEM viewing, but because we
have the only sputter coater on campus and the particular lab doing all
this work requires an extremely heavy layer of whatever metal they are
using---which may be Ni, Pt, or Ta. They are the ones driving the 90 mA
and repeated 4 minute cycles (because 4 minutes is the longest time we
can program into the coater and there is no manual on/off, if we want a
16 minute coat, we have to do 4 runs). This is very heavy use for a
coater designed for general EM use, rather than industrial strength use,
and I am concerned that it's beyond the design parameters of the
instrument and may leave us with a crippled coater (and a crippled lab).
We don't have the funds readily available to replace this machine if it
dies. Normally we run this instrument between 10 and 20 mA for 15
seconds to 3 minutes for SEM coating.

In terms of purging the chamber with argon, etc., this turbo pumped
EmiTech K575X runs on a programmed, automated cycle. The user's role is
to punch in the parameters and push the start button. It has been a
very reliable instrument after some initial setup problems were
corrected. We hope it remains so!

Thanks again, everybody.

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 08:32:59 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 28 Mar 2005 08:45:21 -0600
Subject: [Microscopy] Re: Re: Sputter coating question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Yee Tan,

Normally, according to my information, the lower the coating current,
the finer-grained the coating should be. If this is correct, then if
you need a rather thick, but fine-grained coating for
high-magnification, you should use a low current (5-10 mA) for a long
time (several minutes?). If you are only looking at low magnification,
than a higher current and shorter time should work. Generally speaking,
a lower SEM accelerating voltage should theoretically require a thinner
coating, since it is less likely to cause charging. In my experience,
however, this is not always as straightforward as one would expect and
requires experimentation with each sample. For critical samples, I
always start with the thinnest coating I think might do the job, and
then add more coating as necessary. You can always add more, but
removing it once it is applied is another story.

Coating current is also determined by the metal being used for coating,
since each one has different properties relative to the sputtering
process. Unfortunately, I don't know of a good source where you can
fine this information in one place, but it must exist somewhere.

Regarding the "furry" carbon coating, this sounds like something is
going wrong. I have never seen this, although sometimes when
evaporating carbon with the current too high you can get "chunks" of
carbon flying off the braid or rod and showing up on the sample. The
"furriness" is something else, though. Not sure about that one.

I hope this helps.

Randy
Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








-----Original Message-----
} From: Tay Yee Yan [mailto:one_twinklestar-at-yahoo.com.sg]
Sent: Friday, March 25, 2005 11:44 PM
To: Gary Gaugler; Tindall, Randy D.
Cc: MSA listserver

Hi Gary:

How do you decide which current of the sputter is needed for certain SEM
KeV? Does it apply the same to FESEM? Pardon me for my ignorant because
I just started to use SEM.

Another question is, some recommend to use carbon as coating but
recently when i used it as a coating, i found that my sample is coated
with "furry stuff"
which I believe it should be due to carbon, under FESEM. If I really
need to use carbon coating, what can I do to reduce such "furry stuff"?

Thanks a lot!

Regards,
Yee Yan
Nanyang Technological University
--- Gary Gaugler {gary-at-gaugler.com} wrote:
}
}
}
------------------------------------------------------------------------
------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
-------
}
} I'm using a Denton Desk II coater with Au/Pd or Pt.
} This is for coating semiconductors and other specimens.
} I coat at 20mA for between 30 seconds and 50 seconds depending on
} intended SEM KV. 90mA seems rather high to me. Poor vacuum (}
} 125mTorr) and high current will produce bad coating. It is best, IMO
} to go for longer time at low current and have a good vacuum
} (85-95mTorr).
}
} This works in my SEM from 100V to 8KV at 200pA probe current or less
} without problem.
}
} I don't see why the coater needs to run for 4 minutes.
} What kind is it.
}
} gary g.
}
}
} At 02:47 PM 3/25/2005, you wrote:
}
}
}
} -----------------------------------------------------------------------
} -------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------
} --------
} }
} } We are doing LOTS of sputter coating of various
} metals these days for a
} } group of electrical engineering department clients.
} Up until recently
} } the platinum and titanium coatings that have been
} applied have been
} } shiny and mirror-like, as one would expect. Just
} recently, however, the
} } target has been showing a decidedly scaled, rough
} surface and the
} } coating on the silicon substrate shows microcracks.
} We are using a
} } target that is 0.5mm (0.02") thick, which is
} thicker than the normal
} } target for this machine, but it worked fine when
} installed.
} }
} } The coater is being run very hard----90mA for
} repeated runs of 4 minutes
} } each, often many times a day. It is
} Peltier-cooled, but the target area
} } and photoresist on the sample is heating up,
} although the heat build-up
} } has not been measured. The coater is turbo-pumped.
} }
} } My questions are: what could be causing the scaling
} on the target itself
} } and the microcracks on the surface being coated,
} and will a sputter
} } coating designed for general EM lab use stand up to
} this kind of hard
} } use over time?
} }
} } Thanks for any thoughts.
} }
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility---We Do Small
} Well!
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-5414
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} }
} }
} }
} }
}
}
}

__________________________________________________
Do You Yahoo!?
Log on to Messenger with your mobile phone!
http://sg.messenger.yahoo.com




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 09:35:06 2005



From: Jay Campbell :      microtomy-at-gmail.com
Date: Mon, 28 Mar 2005 09:47:53 -0600
Subject: [Microscopy] LASIK correction- Follow up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello list,
I post this for the many people who asked that I share a summary of
the responses regarding laser vision correction. I would have liked
to have heard from more people that had the procedure (n=6) but those
that did respond were positive without exception. Actual quotes from
those that wrote me included, "The best money I ever spent", "I would
do it again in a heartbeat" and "it changed my life".

Having had the surgery myself last Thursday, I'm inclined to agree.
My vision was 20/200, now corrected to 20/15. There was discomfort
on the first day but by Friday I felt fine. The advice that I would
offer to anyone considering this procedure is to research the doctors
and clinics in your area, a good reputation is generally well
deserved. Also, resist any urge to bargain shop for your eyesight.
The most expensive place isn't always the best, but the cheapest one
is rarely the best....

To the person who questioned whether I was rational to consider
surgery on an otherwise healthy eye "for reasons of vanity" all I can
say is that if you've ever had 20/200 vision, you would know that
there are larger issues than vanity involved.

Thanks to all who responded and big thanks to Nestor for doing the
work that makes this resource possible.
Jay


Jay Campbell
Research Specialist
University of Wisconsin
Laboratory of Molecular Biology
R.M. Bock Labs
1525 Linden Drive
Madison, WI 53706
jmcampbe-at-wisc.edu
608 263 8481


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 10:05:23 2005



From: Alan Stone :      as-at-astonmet.com
Date: Mon, 28 Mar 2005 10:18:15 -0600
Subject: [Microscopy] John Russ Where Are You?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for John Russ for some off-line information. I do not have a
current telephone number.

Alan Stone
ASTON





Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 10:06:28 2005



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Mon, 28 Mar 2005 11:18:33 -0800
Subject: [Microscopy] Re: Fwd: DNA Structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greg-
The low-angle method was developed by Kleinschmidt, and so it is
called after him. It is often used in studies like the one you
describe. There is a protocol posted on our web site. If you look
at the URL at the end of my signature line, you will find it under
TEM protocols.
Carol



} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu
http://www.bgsu.edu/departments/biology/facilities/MnM
___________________________________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 10:16:24 2005



From: Bern.Amy-at-epamail.epa.gov
Date: Mon, 28 Mar 2005 09:22:00 -0700
Subject: [Microscopy] Quantitative Image Analysis for X-ray maps (SEM/EDS)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello list,

I am looking for a quantitative way to analyze X-ray maps for area
percentage of particles of a particlular composition. I have overlayed
X-ray maps, in this case Ca and S, to identify the composition of
interest and I have a backscattered image of the total particles in the
field of view. I want to know what area percent of the particles are
the composition of interest.

I have started looking into LISPIX and Scion Image, but am not familiar
enough with the programs to know what each function is actually doing.
I'm also aware that some EDS software has the capability to do this, but
not all packages do. It would be ideal to use a program that is readily
available to multiple labs, regardless of the system used.

Any help would be appreciated.

Amy

^v^
Amy M. Bern
Chemist
EPA, NEIC, Bldg. 25
P.O. Box 25227
Denver, CO 80225
Phone: 303-462-9128



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 12:11:43 2005



From: AtcSEM :      atcsem-at-sbcglobal.net
Date: Mon, 28 Mar 2005 13:23:18 -0500
Subject: [Microscopy] RE: Quantitative Image Analysis for X-ray maps (SEM/EDS)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

PGT has a system called Spirit that has that capability.
Also if you need to analyze only images you could use ImageJ(free software).
I use ImageJ quite often for image analysis of gamma-prime.


Regards,
Pavel Lozovyy
ATC SEM Lab
(216)692-6637
http://www.atclabs.com/SEM.htm
 
 
 
 


| -----Original Message-----
| From: Bern.Amy-at-epamail.epa.gov [mailto:Bern.Amy-at-epamail.epa.gov]
| Sent: Monday, March 28, 2005 11:22 AM
| To: Microscopy-at-microscopy.com
| Subject: [Microscopy] Quantitative Image Analysis for X-ray maps (SEM/EDS)
|
|
|
| --------------------------------------------------------------------------
| ----
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe --
| http://www.microscopy.com/MicroscopyListserver
| On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
| --------------------------------------------------------------------------
| -----
|
| Hello list,
|
| I am looking for a quantitative way to analyze X-ray maps for area
| percentage of particles of a particlular composition. I have overlayed
| X-ray maps, in this case Ca and S, to identify the composition of
| interest and I have a backscattered image of the total particles in the
| field of view. I want to know what area percent of the particles are
| the composition of interest.
|
| I have started looking into LISPIX and Scion Image, but am not familiar
| enough with the programs to know what each function is actually doing.
| I'm also aware that some EDS software has the capability to do this, but
| not all packages do. It would be ideal to use a program that is readily
| available to multiple labs, regardless of the system used.
|
| Any help would be appreciated.
|
| Amy
|
| ^v^
| Amy M. Bern
| Chemist
| EPA, NEIC, Bldg. 25
| P.O. Box 25227
| Denver, CO 80225
| Phone: 303-462-9128





From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 12:18:52 2005



From: DrJohnRuss-at-aol.com
Date: Mon, 28 Mar 2005 13:30:42 EST
Subject: [Microscopy] Re: Quantitative Image Analysis for X-ray maps (SEM/EDS)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 3/28/05 12:20:55 PM, Bern.Amy-at-epamail.epa.gov writes:

} I am looking for a quantitative way to analyze X-ray maps for area
} percentage of particles of a particlular composition. I have overlayed
} X-ray maps, in this case Ca and S, to identify the composition of
} interest and I have a backscattered image of the total particles in the
} field of view. I want to know what area percent of the particles are
} the composition of interest.
}
} I have started looking into LISPIX and Scion Image, but am not familiar
} enough with the programs to know what each function is actually doing.
} I'm also aware that some EDS software has the capability to do this, but
} not all packages do. It would be ideal to use a program that is readily
} available to multiple labs, regardless of the system used.

You can do that with Photoshop, which is probably already present in most
labs. the procedure is simple:
1. threshold each elemental map to produce a black and white result
delineating where the high/low concentration regions are. If the original image is
pretty noisy you may need to apply a Gaussian blur first. If the area of interest
is white, invert the image so that it is black.
2. copy one image any paste it on top of the other (it will be a layer)
3. set the opacity of the top layer to about 50%. The overlapped area will be
dark.
4.Display the histogram in expanded mode and place your cursor on the line
corresponding to the black pixels. The percentage area that is black, hence
common to the two maps, will be displayed.

For more options and ways to do additional kinds of measurement, check out
the Fovea Pro plugins for Photoshop (www.ReindeerGraphics.com) With these you
can also combine the x-ray maps with the particle image so that the feature
dimensions are taken from the higher resolution SEM image but the composition
information used to select them comes from any Boolean combination of the X-ray
signals.

John Russ




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 12:29:33 2005



From: Long Li :      longli_tem-at-hotmail.com
Date: Mon, 28 Mar 2005 13:41:13 -0500
Subject: [Microscopy] Seeking a post-doctoral research associate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Department of Materials Science and Engineering at the University of
Pittsburgh (UPitt) seeks a post-doctoral research associate for electron
microscopy studies of supported nanoparticles used in heterogeneous
catalysis. Primary research responsibilities include application and
development of scanning transmission electron microscope methods, such as
Z-contrast and in situ, for the structural determination of supported
nanoparticles. The successful candidate will be expected to contribute to
several funded research projects in catalysis, and actively participate in a
stimulating group of faculty, research associates and students.

Heterogeneous catalysis is used widely for chemical production and
environmental protection. Yet, the topology of these nano-sized particles
is quite challenging to determine, but critical to ascertain since catalysis
is a surface reaction. The objective of this research program is the
robust determination of the structural habits of very small nanoparticles
and their energetic landscapes via a coordinated experimental effort, with
novel synthesis (University of Illinois at Urbana-Champaign (UIUC)),
development of (S)TEM (UPitt) and synchrotron X-ray diffraction (Brookhaven
National Laboratory (BNL)) characterization methods, and theoretical and
simulations effort (UIUC). The experimentalists and theorists will interact
closely. Part of the experimental research will be carried out at the
Materials Research Laboratory at UIUC.

A Ph.D. in Materials Science and Engineering, Physics, or related field is
necessary. Required experience includes transmission electron microscopy,
such as Z-contrast, EELS, and HREM. This post-doctoral position is available
starting immediately. The appointment is initially for one year with
possible extension up to 3 years.

To apply for this position, please send a resume with the names and contact
information for three references, or to obtain more information, please
contact:

Professor Judith Yang
848 Benedum Hall
Materials Science & Engineering Dept.
University of Pittsburgh
Pittsburgh, PA 15261

jyang-at-engr.pitt.edu
(412) 624-8613
}


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 12:48:08 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 28 Mar 2005 11:15:59 -0800
Subject: [Microscopy] Re: AskAMicroscopist: microscopy of sand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 25, 2005, at 5:34 PM, by way of MicroscopyListserver wrote:

} Question: I am currently working on a project studying sands under a
} binocular microscope and I am having trouble mounting the sands on
} slides; my poblem is that the epoxy I am using has too many bubbles in
} it and it's too defficults to handle. Can you please suggest what I
} can use instead and/or how I can mount slides without bubbles in
} between the particles?
}
Dear Menachim,
In addition to the other suggestions, perhaps putting a little
super-glue on the glass slide then sprinkling the sand over that would
also work. Since super-glue dries pretty rapidly, it might be much
easier than epoxy to deal with.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 13:08:34 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Mon, 28 Mar 2005 14:21:14 -0500
Subject: [Microscopy] Antibody

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I need to purchase some unconjugated rabbit anti-goat IgG
for immnocytochemistry, and was wondering if anyone on
the list has previous experience with this kind of antibody
and can recommend a source for it. Thanks in advance.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 13:18:19 2005



From: Alan Stone :      as-at-astonmet.com
Date: Mon, 28 Mar 2005 13:31:10 -0600
Subject: [Microscopy] John Russ Inquiry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A blanket thank you to everyone who replied to John's contact information.

Al Stone
ASTON




Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 13:31:06 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Mon, 28 Mar 2005 12:42:16 -0700
Subject: [Microscopy] Quantitative Image Analysis for X-ray maps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Amy,

the task that you are asking for is not quite as simple as it sounds. First
of all you have to identify the particles. That's usually done through some
thresholding and segmentation. Than you have to identify the elements
contained in each particle, which typically requires some morphological
operations and a combination of the EDS and BS data. And finally you have to
calculate the percentage of those particles that are of interest.
We have some add-ins for our software that can do this kind of analysis, but
it probably requires a bit of trial and error to get everything right. If
you'd be interested, we could try to do a sample analysis for you and see if
it fits your needs. Contact be via email or give me a call.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Bern.Amy-at-epamail.epa.gov [mailto:Bern.Amy-at-epamail.epa.gov]
Sent: Monday, March 28, 2005 09:22
To: Microscopy-at-microscopy.com

Hello list,

I am looking for a quantitative way to analyze X-ray maps for area
percentage of particles of a particlular composition. I have overlayed
X-ray maps, in this case Ca and S, to identify the composition of
interest and I have a backscattered image of the total particles in the
field of view. I want to know what area percent of the particles are
the composition of interest.

I have started looking into LISPIX and Scion Image, but am not familiar
enough with the programs to know what each function is actually doing.
I'm also aware that some EDS software has the capability to do this, but
not all packages do. It would be ideal to use a program that is readily
available to multiple labs, regardless of the system used.

Any help would be appreciated.

Amy

^v^
Amy M. Bern
Chemist
EPA, NEIC, Bldg. 25
P.O. Box 25227
Denver, CO 80225
Phone: 303-462-9128




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 14:02:14 2005



From: Long Li :      longli_tem-at-hotmail.com
Date: Mon, 28 Mar 2005 15:15:17 -0500
Subject: [Microscopy] A postdoc position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Department of Materials Science and Engineering at the University of
Pittsburgh (UPitt) seeks a post-doctoral research associate for electron
microscopy studies of supported nanoparticles used in heterogeneous
catalysis. Primary research responsibilities include application and
development of scanning transmission electron microscope methods, such as
Z-contrast and in situ, for the structural determination of supported
nanoparticles. The successful candidate will be expected to contribute to
several funded research projects in catalysis, and actively participate in a
stimulating group of faculty, research associates and students.

Heterogeneous catalysis is used widely for chemical production and
environmental protection. Yet, the topology of these nano-sized particles
is quite challenging to determine, but critical to ascertain since catalysis
is a surface reaction. The objective of this research program is the
robust determination of the structural habits of very small nanoparticles
and their energetic landscapes via a coordinated experimental effort, with
novel synthesis (University of Illinois at Urbana-Champaign (UIUC)),
development of (S)TEM (UPitt) and synchrotron X-ray diffraction (Brookhaven
National Laboratory (BNL)) characterization methods, and theoretical and
simulations effort (UIUC). The experimentalists and theorists will interact
closely. Part of the experimental research will be carried out at the
Materials Research Laboratory at UIUC.

A Ph.D. in Materials Science and Engineering, Physics, or related field is
necessary. Required experience includes transmission electron microscopy,
such as Z-contrast, EELS, and HREM. This post-doctoral position is available
starting immediately. The appointment is initially for one year with
possible extension up to 3 years.

To apply for this position, please send a resume with the names and contact
information for three references, or to obtain more information, please
contact:

Professor Judith Yang
848 Benedum Hall
Materials Science & Engineering Dept.
University of Pittsburgh
Pittsburgh, PA 15261

jyang-at-engr.pitt.edu
(412) 624-8613


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 14:17:58 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 28 Mar 2005 12:45:52 -0800
Subject: [Microscopy] Re: Imaging Quantum dots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 25, 2005, at 6:51 PM, Debby Sherman wrote:

} I would appreciate tips on imaging 2-5 nm quantum dots as we have not
} done
} this in the past. We will do the imaging using a Philips CM-100 TEM
} configured for high resolution. I am assuming that a carbon film
} support
} grid would be preferable but what about contrasting?
}
Dear Debby,
We have done some imaging of quantum dots in this size range on our
T12, which is similar enough to your CM-100. First, make sure you have
good coverage, since the QDs are so small that they do not show up at
lower mags where you have a wide field of view. You will only be able
to scan your grid at high mag, and, even there, the QDs are fairly low
contrast, so they are hard to distinguish unless you are pretty near
focus--just slightly underfocus works best--and this requires that you
have a pretty flat grid. If these conditions are met, the rest is
perseverance. You are correct that carbon support film is good, and
the thinner and more uniform it is the better. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 15:18:58 2005



From: Damian Neuberger :      neuberger1234-at-comcast.net
Date: Mon, 28 Mar 2005 17:26:18 -0600
Subject: [Microscopy] 18% gray by digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I think the Moran Scientific 'Chemical Imaging' x-ray mapping software may do what you
want (http://www.goulburn.net.au/~kmoran/ ), but I don't know whether it would be
possible to purchase only that part of their software suite.

It might be worthwhile asking them, though (kmoran-at-goulburn.net.au).

I have no connection with Moran Scientific other than being a satisfied customer.

cheers

rtch



Date sent: Mon, 28 Mar 2005 09:22:00 -0700
} From: Bern.Amy-at-epamail.epa.gov

Can anyone tell me if digital camera light meters work in a manner similar
to those in older film cameras (with built-in meters) and handheld light
meters?

As I recall, film cameras with built in light meters and handheld light
meters determined the proper exposure as if the scene were 18% gray. That
is why I would set the exposure using an 18% gray card for subjects that
were difficult to determine the exposure. Now I wonder if digital SLR and
consumer cameras also do the same thing, i.e. do they determine the exposure
as if the scene or object were equivalent to an 18% gray. Does it make any
difference whether one is using spot, average, center weighted metering
modes, given that all areas of the scene or object are relatively similar
and that there are no huge differences from one portion of the field of view
and another? Yes, I know that if I have an dark object of interest against
a white background, it is preferable to use spot metering.

TIA

Damian Neuberger




From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 17:46:04 2005



From: Vlad Speransky :      vladislav_speransky-at-nih.gov
Date: Mon, 28 Mar 2005 18:57:58 -0500
Subject: [Microscopy] Re: printing directory list from CD ROM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

A free program called TextWrangler
http://www.barebones.com/products/textwrangler/index.shtml
seems the easiest solution. You launch TextWrangler and simply drag and
drop the CD icon from your Desktop (or any other folder) into the new
document window, and that's it. All files listed, with all subfolder
hierarchy shown by multilevel indentation. You can keep dragging more
CD listings into the same window, of course. Save as default, and it
will open and look nice in any word processor, including MS Word, in
case you need that.

Vlad Speransky, Staff Scientist
Supramolecular Structure and Function Resource
Division of Bioengineering and Physical Science
ORS, National Institutes of Health
13 South Dr, Rm. 3N17
Bethesda, MD 20892-5766
301 496-3989
vladislav_speransky-at-nih.gov



} We have a large number of CD ROMS and need to generate a printout of
} the files on the disks.
}
} How can we do this on a Mac running OSX 10.3?
}
} Thank you very much.
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}



From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 19:52:25 2005



From: avander-at-uoguelph.ca (by way of MicroscopyListserver)
Date: Tue, 29 Mar 2005 12:00:55 +1000
Subject: [Microscopy] viaWWW: fluorescence in situ hybridization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (avander-at-uoguelph.ca) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
March 28, 2005 at 10:42:02
---------------------------------------------------------------------------

Email: avander-at-uoguelph.ca
Name: Amanda van der Vinne

Organization: University of Guelph

Title-Subject: [Microscopy] [Filtered] Autofluorescence in bacteria

Question: Hi,

I am trying to perform fluorescence in situ hybridization with
bacterial cells. Currently I am working with E. coli and S.
typhimurium. I am seeing extremely high levels of autofluorescence
in both cell types no matter what I have used to fix the cells. I
have tried fixing the cells with 4% paraformaldehyde, ethanol,
methanol, a methanol/acetic acid mix as well as 1M HCl. It will be
impossible to detect the fluorescence of a probe, with the level of
autofluorescence that I am seeing. Has anybody seen this before?
Does anybody have any ideas of how to decrease this autofluorescence?

Thanks for your help!

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 19:52:15 2005



From: dcrippen-at-buckinstitute.org (by way of MicroscopyListserver)
Date: Tue, 29 Mar 2005 12:03:16 +1000
Subject: [Microscopy] viaWWW: Ti:sapphire Coherent or Spectrum Physics Lasers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dcrippen-at-buckinstitute.org) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Monday, March 28, 2005 at 14:18:40
---------------------------------------------------------------------------

Email: dcrippen-at-buckinstitute.org
Name: Danielle Crippen

Organization: Buck Institute for Age Research

Title-Subject: [Microscopy] [Filtered] MListserver: Ti:sapphire Coherent or Spectrum Physics

Question: Dear all,

We are planning to purchase a Zeiss 510 NLO for our Core facility and
are having trouble distinguishing and deciding between the Coherent
and Spectrum Physics lasers. There was a deep discussion in this
forum on this topic in 1998, but we assume (perhaps naively) the
lasers have changed/advanced since then.

Keeping in mind we are a multi-user facility, our main considerations
are: 1.) ease of use, 2.) stability, and 3.) robustness.

Any and all experience and expertise is welcome!

Many thanks in advance,

Danielle Crippen

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Mar 28 22:28:49 2005



From: narasimhanpotti-at-hotmail.com (by way of MicroscopyListserver)
Date: Tue, 29 Mar 2005 14:41:36 +1000
Subject: [Microscopy] viaWWW: LigninSuberin autofluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (narasimhanpotti-at-hotmail.com) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Monday, March 28, 2005 at 20:11:16
---------------------------------------------------------------------------

Email: narasimhanpotti-at-hotmail.com
Name: Narasimhan

Organization: University of Kerala

Title-Subject: [Microscopy] [Filtered] LigninSuberin autofluorescence

Question: Dear All,

How one can specifically detect autofluorescence of lignin and
suberin in plant cells? What is its colour of fluorescence and wha is
the emission maxima and asborption maxima for each

Thanks
Narasimhan

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 00:06:22 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 28 Mar 2005 22:20:19 -0800
Subject: [Microscopy] Re: 18% gray by digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Damian
As far as I could understand, modern SLR cameras (film) measure lighting in
very complicated manner: they measured in many areas and then applied some
"weighting" rules. In simplest case, the gaussian distribution applied, so
central area have more weight than periphery (differ from
spot-metering). But again - it's much more complicated and they have
different modes (for portrait or landscape etc). I would imagine modern
sophisticated digital cameras do approximately the same. From another
hand, the dynamic range of CCD chips is much wider than film, so you
basically don't need to do precise exposure to have decent results. I
would imagine that in cheap consumer cameras they have two or three
"exposure diapason" and ruffly determine in which "diapason" lighting
condition fitted - keep in mind, most of them used flash automatically.
When picture is taken they just simply normalized it to some average
numbers ( like 18% gray rule). This is just my speculation. Sergey

At 03:26 PM 3/28/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 00:55:33 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 28 Mar 2005 23:09:33 -0800
Subject: [Microscopy] Re: Re: Fwd: DNA Structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gregory

The most trickiest part in any DNA visualization is a sample preparation.
DNA tends do not spread well, so you may have a huge difficulties to
recognize specific DNA structures like hairpins, etc. Kleinschmidt
technique may destroy 3D structure because based on "spreading" approach -
"spreading" forces will "spread" some of your 3D structure as well. As
soon as you established good sample-preparation technique, shadowing and
AFM both will produce a quite decent results. You need to keep in mind
that in AFM, the probe could move the DNA, so DNA should be anchored to the
surface pretty well to avoid artefacts. From my point of view, shadowing
is better if you need to do some statistics - you may collect hundreds of
images quite easily. Shadowing also resolves the DNA overlapping like knots
etc. AFM is not good for this. Basically, I do believe that the
combination of both would be very beneficial.

Traditionally, people do Pt (or Pt alloys) shadowing, which delivers 6 nm
resolution. You could not see well the structure of 3 nm DNA using Pt.
Kleinschmidt technique is even worse: it adds a thick layer of protein, so
you basically see something, which supposed to be a "DNA", 10-15 nm
thick... If you need relatively good resolution, you need to adsorb DNA
directly on mica (there are bunch of ways how to do so has been already
published) and shadow with tungsten. Tungsten-shadowing delivers about
0.7-1 nm resolution, so you could see a real structure, not a sausage 3-4x
thicker than actual structure.

With support from NSF I happens to build special vacuum evaporator for
high-resolution low-angle tungsten shadowing. It was cost to US taxpayers
$383K. It permits to shadow at 0.5-90 deg with increment 0.5 deg,
two-directional shadowing, rotary shadowing, freeze-drying etc. The vacuum
is 5x10-7 torr in 20 min from air (10-8 after a few hour). It has two
home-made Electron guns to evaporate two materials (carbon and W normally).
It also has thickness Monitor for precise monitoring the thickness of
evaporated material (0.01 nm -yes 0.01, believe or not). Normally I do
"indirect replica" when DNA adsorbed on mica, then shadowed with W (1-1.5
nm thickness) at 5 deg (rotary or two-directional). On top of W I evaporate
1.5-2 nm of carbon at 70 deg (rotary). Then I removed carbon layer (with
buried in it W "replica") from mica (on the water) and mount it on the EM
grids with holey film on it. The disadvantage of W shadowing is that the
image-contrast degraded in couple of hours, so you need to take pictures
ASAP. You also need to have some special equipment. If you have some
particular questions, you may contact off line. Sergey

At 11:18 AM 3/28/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 04:29:49 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Tue, 29 Mar 2005 07:11:57 -0330
Subject: [Microscopy] RE: 18% gray by digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Damian Neuberger writes ...

} Can anyone tell me if digital camera light meters work
} in a manner similar to those in older film cameras (with
} built-in meters) and handheld light meters?
}
} As I recall, film cameras with built in light meters and
} handheld light meters determined the proper exposure as if
} the scene were 18% gray. ...

Like the other post, I cannot speak as to the specific technique for
digital evaluating the exposure ... and it's likely somewhat different,
manufacturer to manufacturer. That said, one thing that is assured is that
digital cameras do NOT have to employ the same or similar method. The 18%
gray card, which would also include the technique incident light meters use,
were all aimed at the capabilities of film, and for a given film
sensitivity. CCD and CMOS sensors offer much different capabilities, and in
many cases a greater dynamic range. Illumination algorithms therefore only
need keep all pixels values within the hardware's dynamic range (e.g., 8bit,
10, bit, 12bit, 14bit), for a desired level of noise (the d-cam's pseudo-ASA
setting).

hth & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 08:20:32 2005



From: Long Li :      longli_tem-at-hotmail.com
Date: Tue, 29 Mar 2005 09:33:36 -0500
Subject: [Microscopy] A Postdoc Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Department of Materials Science and Engineering at the University of
Pittsburgh (UPitt) seeks a post-doctoral research associate for electron
microscopy studies of supported nanoparticles used in heterogeneous
catalysis. Primary research responsibilities include application and
development of scanning transmission electron microscope methods, such as
Z-contrast and in situ, for the structural determination of supported
nanoparticles. The successful candidate will be expected to contribute to
several funded research projects in catalysis, and actively participate in a
stimulating group of faculty, research associates and students.

Heterogeneous catalysis is used widely for chemical production and
environmental protection. Yet, the topology of these nano-sized particles
is quite challenging to determine, but critical to ascertain since catalysis
is a surface reaction. The objective of this research program is the
robust determination of the structural habits of very small nanoparticles
and their energetic landscapes via a coordinated experimental effort, with
novel synthesis (University of Illinois at Urbana-Champaign (UIUC)),
development of (S)TEM (UPitt) and synchrotron X-ray diffraction (Brookhaven
National Laboratory (BNL)) characterization methods, and theoretical and
simulations effort (UIUC). The experimentalists and theorists will interact
closely. Part of the experimental research will be carried out at the
Materials Research Laboratory at UIUC.

A Ph.D. in Materials Science and Engineering, Physics, or related field is
necessary. Required experience includes transmission electron microscopy,
such as Z-contrast, EELS, and HREM. This post-doctoral position is available
starting immediately. The appointment is initially for one year with
possible extension up to 3 years.



To apply for this position, please send a resume with the names and contact
information for three references, or to obtain more information, please
contact:



Professor Judith Yang
848 Benedum Hall
Materials Science & Engineering Dept.
University of Pittsburgh
Pittsburgh, PA 15261

jyang-at-engr.pitt.edu
(412) 624-8613




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 08:20:32 2005



From: Long Li :      longli_tem-at-hotmail.com
Date: Tue, 29 Mar 2005 09:33:36 -0500
Subject: [Microscopy] A Postdoc Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Department of Materials Science and Engineering at the University of
Pittsburgh (UPitt) seeks a post-doctoral research associate for electron
microscopy studies of supported nanoparticles used in heterogeneous
catalysis. Primary research responsibilities include application and
development of scanning transmission electron microscope methods, such as
Z-contrast and in situ, for the structural determination of supported
nanoparticles. The successful candidate will be expected to contribute to
several funded research projects in catalysis, and actively participate in a
stimulating group of faculty, research associates and students.

Heterogeneous catalysis is used widely for chemical production and
environmental protection. Yet, the topology of these nano-sized particles
is quite challenging to determine, but critical to ascertain since catalysis
is a surface reaction. The objective of this research program is the
robust determination of the structural habits of very small nanoparticles
and their energetic landscapes via a coordinated experimental effort, with
novel synthesis (University of Illinois at Urbana-Champaign (UIUC)),
development of (S)TEM (UPitt) and synchrotron X-ray diffraction (Brookhaven
National Laboratory (BNL)) characterization methods, and theoretical and
simulations effort (UIUC). The experimentalists and theorists will interact
closely. Part of the experimental research will be carried out at the
Materials Research Laboratory at UIUC.

A Ph.D. in Materials Science and Engineering, Physics, or related field is
necessary. Required experience includes transmission electron microscopy,
such as Z-contrast, EELS, and HREM. This post-doctoral position is available
starting immediately. The appointment is initially for one year with
possible extension up to 3 years.



To apply for this position, please send a resume with the names and contact
information for three references, or to obtain more information, please
contact:



Professor Judith Yang
848 Benedum Hall
Materials Science & Engineering Dept.
University of Pittsburgh
Pittsburgh, PA 15261

jyang-at-engr.pitt.edu
(412) 624-8613




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 08:58:44 2005



From: Michal Jarnik :      M_Jarnik-at-fccc.edu
Date: Tue, 29 Mar 2005 10:11:32 -0500
Subject: [Microscopy] Psoralen crosslinking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I would need to (probably psoralen/UV) crosslink in vitro replication
products for further spreading/shadowing. There is quite a lot of
literature available about the method, but they mostly deal with
crosslinking in vivo and in any case, first hand experience is always
helpful. I would appreciate ideas for (if possible) something not very
equipment demanding (I do not have any special UV lamp, so methods using
e.g. the crosslinking oven used for hybridization membranes would be
great).

Thanks,

Michal Jarnik,
EM Facility
Fox Chase Cancer Center
Phila., PA



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 11:09:05 2005



From: R. Howard Berg :      rhberg-at-danforthcenter.org
Date: Tue, 29 Mar 2005 11:21:38 -0600
Subject: [Microscopy] Re: viaWWW: LigninSuberin autofluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Narasimhan,

These polymers are excited by blue wavelengths and, with two photon 790
nm excitation of Arabidopsis tissues they have similar, broad
emissions when measured on our Zeiss META spectral detector. As shown
in the paper cited below, the emission for
lignin/suberin/cutin/sporopollenin has a broad peak around 480-510 nm.

Berg, R.H. (2004) Evaluation of spectral imaging for plant cell
analysis. J Microscopy 214: 174-181

Howard

On Mar 28, 2005, at 10:41 PM, by way of MicroscopyListserver wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
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}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (narasimhanpotti-at-hotmail.com) from
} http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
} Monday, March 28, 2005 at 20:11:16
} -----------------------------------------------------------------------
} ----
}
} Email: narasimhanpotti-at-hotmail.com
} Name: Narasimhan
}
} Organization: University of Kerala
}
} Title-Subject: [Microscopy] [Filtered] LigninSuberin autofluorescence
}
} Question: Dear All,
}
} How one can specifically detect autofluorescence of lignin and suberin
} in plant cells? What is its colour of fluorescence and wha is the
} emission maxima and asborption maxima for each
}
} Thanks
} Narasimhan
}
} -----------------------------------------------------------------------
} ----
}
}
R. Howard Berg, Ph.D.
Director, Integrated Microscopy Facility
Danforth Plant Science Center
975 N. Warson Rd.
St. Louis, MO 63132

ph 314-587-1261 fx 314-587-1361 cell 314-378-2409
rhberg-at-danforthcenter.org www.danforthcenter.org
visit this educational resource: http://www.danforthcenter.org/Cells/



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 13:16:17 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 30 Mar 2005 10:42:56 +1200
Subject: [Microscopy] EDS repump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sorry for spaming. Since it didn't show up first, but all showed up same
time





Long Li
_________________________________________________________
Materials Science and Engineering Department
University of Pittsburgh
3700 O'Hara St., 848 Benedum Hall
Pittsburgh, PA 15261


----- Original Message -----
} From: "Long Li" {longli_tem-at-hotmail.com}
To: {microscopy-at-ns.microscopy.com} ; "Microscopy-at-msa. microscopy. com"
{Microscopy-at-msa.microscopy.com}
Sent: Tuesday, March 29, 2005 9:33 AM

Sorry for spaming. Since it didn't show up first, but all showed up same
time





Long Li
_________________________________________________________
Materials Science and Engineering Department
University of Pittsburgh
3700 O'Hara St., 848 Benedum Hall
Pittsburgh, PA 15261


----- Original Message -----
} From: "Long Li" {longli_tem-at-hotmail.com}
To: {microscopy-at-ns.microscopy.com} ; "Microscopy-at-msa. microscopy. com"
{Microscopy-at-msa.microscopy.com}
Sent: Tuesday, March 29, 2005 9:33 AM


Is this truly so?

That CCDs have a wider dynamic range than film?

My amateurish and limited experience had led me to the conclusion that, with digital
images, dimming overexposed images, or brightening underexposed ones, doesn't
reveal any hoped-for detail, unlike film.

But maybe this is somehow a consequence of the capture and subsequent processing
that goes on between the CCD and my monitor screen.

cheers

rtch



Date sent: Mon, 28 Mar 2005 22:20:19 -0800
To: Microscopy-at-microscopy.com
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}

Hi

Having just found out the hard way that my EDS detector wouldn't make it through a 3-
day weekend without a LN2 topup, I guess it's now my turn to learn how to repump a
detector.

Electrically it's still fine, resolution what it was last week, but the LN2 now is boiling
gently but continuously, so I suppose the getter has released whatever it had got,
further degrading the dewar vacuum, which had been on a downhill path since it's last
factory pump 'n' bake a couple of years ago.

As my dealings with the factory have always been pretty labored and unsatisfactory (it
should have lasted more than a couple of years after a factory pump 'n' bake, in my
opinion), this seems like the ideal time to use the vacuum port adaptor which I bought
from them.

Does anyone know what I need to get the dewar vacuum down (or up) to for adequate
performance?

I plan to use the SEM vacuum, via an adaptor I've had made for the sample instertion
port, but of course the adaptors, valves and tubing do compromise the vacuum
somewhat.

I am toying with the idea of putting a high-quality shutoff valve at the detector instead of
the factory port, with a tube permanently leading to the SEM chamber, so that I can re-
evacuate the dewar at will by simply opening the valve. Any comments on that idea?

For you lucky people in Europe or the USA it's probably no big deal to send an EDS
detector off to a factory service centre for a quick pump 'n' bake, but if you look at a
map or globe, you can see what a major logistical exercise it is for me.

Helpful tips and comments solicited.

cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 17:34:34 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 29 Mar 2005 16:02:27 -0800
Subject: [Microscopy] Re: Re: Re: 18% gray by digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 29, 2005, at 12:53 PM, Ritchie Sims wrote:

} Is this truly so?
}
} That CCDs have a wider dynamic range than film?
}
} My amateurish and limited experience had led me to the conclusion
} that, with digital
} images, dimming overexposed images, or brightening underexposed ones,
} doesn't
} reveal any hoped-for detail, unlike film.
}
} But maybe this is somehow a consequence of the capture and subsequent
} processing
} that goes on between the CCD and my monitor screen.
}
Dear Ritchie,
CCds do, indeed, have a wider dynamic range and better linearity than
film. Our CCD maxes out at 16k counts with a lower limit--as
determined from the standard deviation of a dark reference--about 1000
times less. While it is true that film can be calibrated so that
quantitative information can be obtained from its non-linear response
region, that information will not have the resolution that is obtained
from the linear region. I.e., for ODs of ~0.1 to ~2 (depending on the
film) the number of electrons per pixel can be determined to greater
precision than for ODs of ~2 to ~4 (which is the highest OD I have been
able to record, so one can only say that more than X electrons were
incident on that pixel). Depending on your film scanner, you may or
may not be able to extract all the information from the film, so by
working in the darkroom you may be able to reveal hitherto hidden
detail that you cannot see in the scanned image. The better linearity
of the CCD will reveal the detail initially, if you choose the optimal
settings for contrast and brightness (or, better still, gamma), so you
will not usually be able to extract more detail, just make it more
apparent. Some of those on the list who are more knowledgeable than I
might be able to correct any mistakes I've made here.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 18:47:17 2005



From: Philip Flaitz :      flaitz-at-us.ibm.com
Date: Tue, 29 Mar 2005 19:59:11 -0500
Subject: [Microscopy] Metropolitan Microscopy Society (NY/NJ) Spring Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







Metropolitan Microscopy Society Spring Meeting 2005
=======================================================

Date: Friday, April 22, 2005
Time: 8:00 am (registration begins)
Place: Carl Zeiss SMT, One Zeiss Drive, Thornwood, NY
914-747-7700 (Directions available on request)

Registration: $20 (PRE-REGISTRATION REQUIRED by April 18)
-------------------------


· 8:00 – 9:15 Registration and Coffee

· 9:15 – 9:30 Introduction
Phil Flaitz, President Metropolitan Microscopy Society

· 9:30 – 10:15 Application of FIB and EBSD to the analysis of fine
grained materials
Joe Michael, MAS Sponsored Tour Lecturer, Sandia Nat’l Lab

· 10:15 – 11:00 ASTM Standards in the SEM.
John Friel, Princeton Gamma Tech Instruments, Rocky Hill,
NJ

· 11:00 – 11:15 Break

· 11:15 – 12:00 3D Reconstruction and End Point Detection: Advantages
of Real Time Imaging in a Crossbeam
Ed Principe, Zeiss SMT, Thornwood, NY


· 12:00 – 1:00 Lunch (included with registration –
please pre-register!)


· 1:00 - 1:30 Zeiss Facility Tour

· 1:30 – 2:15 Backscattered Electron Imaging of Subsurface Cu
Interconnect
Structures
Lynne Gignac, IBM Research Division, Yorktown Heights, NY

· 2:15 - 3:00 Backscatter Electron (BSE) Imaging in the SEM using an
Electron Backscattering Pattern (EBSP) Detector Array
Oliver Wells, IBM Research Division, Yorktown Heights, NY


==========================================================

For registration contact:
Evan Slow --- (201) 760-2524
eslow-at-angstrom.us

==========================================================




Philip L. Flaitz
IBM Microelectronics, Hopewell Junction, NY
Ph.......(845) 892-3094, FAX -6256
pager - 800-352-4732, PIN# 1121
flaitz-at-us.ibm.com



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 19:00:35 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 29 Mar 2005 17:12:45 -0800
Subject: [Microscopy] Re: 18% gray by digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A short excursion into the Zone System might clear
up some of these issues. However, dynamic range
is it seems, a different factor.

With a digicam, the main variables are ISO (A/D
gain and binning) and resolution (total pixel count).
As ISO increases, so does noise...irrespective of
bit width. Consequently, film with blown out highlights
is not going to produce any information in the blown out
areas. No data, no results. Dimming these are not going
to produce valid data. If the exposure is not within
the linear region of the medium, then all information
outside of this will be lost (or very difficult to retrieve,
if at all). The factor here is D, or density. This is
usually a big deal issue with scanners. One would like the
highest Dmax possible. It is a log function. Dmax of
4 is quite good. Cheap scanners are 3.2 or thereabout at
best.

Bad digital pix are bad either due to over or under-exposure.
Same for film. The difference is that most users seem to
use 8-bit images. Some even cling to JPEG! The best, IMO,
way to do this is to deal only in 16-bit TIFF images as a
final result. For intermediary files, use RAW (like Nikon NEF).
Then, use Optipix or Bibble to expand these to full value.
Once optimized, they can be reduced to 8-bits. This also holds
true for SEM capture. 16-bit is best. Then process and finally
reduce to 8-bits for public consumption. This of course assumes
that your data capture hardware does more than 8-bits native.
If not, you have only 256 shades of grey to work with. Not good.
Your highlights will be gone and there will be no detail in the
shadows (dark areas).

gary g.



At 12:53 PM 3/29/2005, you wrote:

} Is this truly so?
}
} That CCDs have a wider dynamic range than film?
}
} My amateurish and limited experience had led me to the conclusion that,
} with digital
} images, dimming overexposed images, or brightening underexposed ones, doesn't
} reveal any hoped-for detail, unlike film.
}
} But maybe this is somehow a consequence of the capture and subsequent
} processing
} that goes on between the CCD and my monitor screen.
}
} cheers
}
} rtch
}
}
}
} Date sent: Mon, 28 Mar 2005 22:20:19 -0800
} To: Microscopy-at-microscopy.com
} } From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: [Microscopy] Re: 18% gray by digital cameras
}
} }
} }
} } ----------------------------------------------------------------------
} } -------- The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help



From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 19:13:30 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 29 Mar 2005 17:41:24 -0800
Subject: [Microscopy] Re: EDS repump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 29, 2005, at 2:42 PM, Ritchie Sims wrote:

} Having just found out the hard way that my EDS detector wouldn't make
} it through a 3-
} day weekend without a LN2 topup, I guess it's now my turn to learn how
} to repump a
} detector.
}
} Electrically it's still fine, resolution what it was last week, but
} the LN2 now is boiling
} gently but continuously, so I suppose the getter has released whatever
} it had got,
} further degrading the dewar vacuum, which had been on a downhill path
} since it's last
} factory pump 'n' bake a couple of years ago.
}
} As my dealings with the factory have always been pretty labored and
} unsatisfactory (it
} should have lasted more than a couple of years after a factory pump
} 'n' bake, in my
} opinion), this seems like the ideal time to use the vacuum port
} adaptor which I bought
} from them.
}
} Does anyone know what I need to get the dewar vacuum down (or up) to
} for adequate
} performance?
}
} I plan to use the SEM vacuum, via an adaptor I've had made for the
} sample instertion
} port, but of course the adaptors, valves and tubing do compromise the
} vacuum
} somewhat.
}
} I am toying with the idea of putting a high-quality shutoff valve at
} the detector instead of
} the factory port, with a tube permanently leading to the SEM chamber,
} so that I can re-
} evacuate the dewar at will by simply opening the valve. Any comments
} on that idea?
}
} For you lucky people in Europe or the USA it's probably no big deal to
} send an EDS
} detector off to a factory service centre for a quick pump 'n' bake,
} but if you look at a
} map or globe, you can see what a major logistical exercise it is for
} me.
}
} Helpful tips and comments solicited.
}
Dear Ritchie,
When I repumped the detector on a TEM, I replaced the back panel that
came with the detector with one that had a high quality shutoff valve,
and I created a branch line off the column vacuum line. The column
vacuum was ~10^-6 torr at the ion gauge, but I didn't measure it nearer
the detector. This worked for many years to restore the resolution of
the detector to its specified value. I had to remove the LN2 from the
dewar, and, of course, make sure that the bias stayed off while the
detector was warm. For a more complete regeneration, I might have
tried heating the dewar by filling it with boiling water, but this
would have been awkward, and, since I got good resolution without it, I
never heated the dewar beyond room temp. I should also say that this
was a SiLi with 148 eV specified resolution, so my method might not be
satisfactory for a newer type of detector. BTW, even in the USA it was
a big deal to dismount and ship the detector for service, and one time
the shipping company damaged the detector on its return voyage.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 19:51:58 2005



From: Andrew Buechele :      andrewb-at-vsl.cua.edu
Date: Tue, 29 Mar 2005 21:03:36 -0500
Subject: [Microscopy] Re: EDS repump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On 30 Mar 2005 at 10:42, Ritchie Sims wrote:

} Does anyone know what I need to get the dewar vacuum down (or
up) to for
} adequate performance?

} I have done this on two detectors. I used a small vacuum system
with an air cooled diffusion pump and a simple LN2 cold trap. The
dessicant in the dewar is usually located around the neck, so I
applied a heat gun to that area while I was pumping. Be sure the
dewar is empty and has warmed thoroughly before you begin, or
you will be fighting a losing battle. Also, BE SURE that the outside of
the detector window is at atmospheric pressure before you start the
warmup or there is a danger of rupturing the window as the
condensed matter in the dewar vaporizes; the windows can't take
much reverse pressure. (UTW'S are probably the only concern
here, Be-windows are probably not going to be ruptured by any
internal pressure likely to be attained during warmup even if looking
at column vacuum.)

} I plan to use the SEM vacuum, via an adaptor I've had made for
the sample
} instertion port, but of course the adaptors, valves and tubing do
} compromise the vacuum somewhat.
}
It will should work, but remember that whatever you pull out of the
detector dewar (mostly water) is going to wind up in your
microscope vacuum system. Putting a cold trap in your pumping line
would take care of that, and improve the pumping.

} I am toying with the idea of putting a high-quality shutoff valve at
the
} detector instead of the factory port, with a tube permanently
leading to
} the SEM chamber, so that I can re- evacuate the dewar at will by
simply
} opening the valve. Any comments on that idea?

Again, it should work, but note the caveats above.

All the best,

Andy Buechele, Washington, D.C.,
U.S.A.




From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 20:53:15 2005



From: Larry Allard :      allardlfjr-at-ornl.gov
Date: Tue, 29 Mar 2005 22:07:41 -0500
Subject: [Microscopy] interesting specimen prep problem...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers:

I'll buy lunch in Hawaii at M&M this summer for whomever contributes
the best solution to this problem:

I have a 360 micron diameter silica fiber, 75 mm long, with a 50
micron diameter channel down the center of the fiber. The "stuff"
in the channel is of interest, so we'd like to find the best way to
get into the central channel for SEM or microprobe imaging/analysis
of the morphology and elemental distribution of the stuff in the
channel. Mounting and grinding is considered out of the question, as
it would be impossible to avoid debris from the grinding process
getting into the channel. FIB milling and ion milling also don't
seem to be useful solutions. My best solution so far is to give up
on the longitudinal section idea, and go for fracture cross-sections,
which we think we can do by positioning the fiber in a jig (say a
disk with a hole slightly larger than the fiber drilled through it),
and simply breaking off a protruding tip and then looking at the
cross-section in the SEM or probe. This would probably produce a
clean section, and we could fracture off maybe 1mm lengths at a time
and look at the cross sections with 1mm resolution down the length of
the fiber. A bit of a hassle, but doable, I think. But I don't want
to give up on the longitudinal section idea, so any tips or
suggestions from the field would be very welcome. Pls reply
off-line; I'll be glad to post any solution that ends up working.

advTHANKSance...

Larry :-)
--
Dr. Lawrence F. Allard
Distinguished Research Staff Member
High Temperature Materials Laboratory
Microscopy, Microanalysis, Microstructures Group
Metals and Ceramics Division
Oak Ridge National Laboratory
1 Bethel Valley Road
PO Box 2008
Oak Ridge, TN 37831-6064
(note: the last 4 lines are sufficient for mailing or overnight
courier service)
865-574-4981
865-576-5413 Fax
http://www.ms.ornl.gov/htmlhome/mauc


From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 22:12:38 2005



From: Damian Neuberger :      neuberger1234-at-comcast.net
Date: Tue, 29 Mar 2005 22:24:16 -0600
Subject: [Microscopy] RE: Re: 18% gray by digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My thanks to the several replies to this thread. The additional
consideration of scanning EM negatives was not initially a consideration but
worth thinking about. Originally, it is the participation in a
multigenerational scientific project using digital cameras (yes, it's that
big and will take that long) when I took on the responsibility of
calibrating the monitors and printers when I realized that the people (3 so
far) involved in this will be using their own cameras. So I had to think
about calibration of the cameras to create ICC profiles, and so on. That is
where I began to wonder if digital cameras determined the exposure the same
way for CCD chips as they do for film considering the wider dynamic range
sensitivities and probably other factors. I know that each manufacturer
will have their own algorithms for post processing which is probably even a
greater issue, even between different models from the same mfg. which I have
now seen, this especially true for the "matrix" type of metering which gives
different areas of the image different weights.

I wanted to have everyone use the RAW image format which does the least or
no in-camera processing, but mine is the only one that will allow that.
BTW, the Nikon NEF is 16 bit per channel (on a D1X) and can be opened in
Photoshop CS by using the OPEN AS command and selecting the correct format,
doing as much of the processing as can be done before converting to 8-bit.
I guess that in one respect film is the same as digital in that if there is
no data, there is nothing you can do about it. Well, maybe with film you
can if you know you under or overexposed you can try to adjust development
time and temp. I'm not sure there is anything you can do with the output of
a CCD chip.

Thanks again for the information,

Damian Neuberger





A short excursion into the Zone System might clear
up some of these issues. However, dynamic range
is it seems, a different factor.

With a digicam, the main variables are ISO (A/D
gain and binning) and resolution (total pixel count).
As ISO increases, so does noise...irrespective of
bit width. Consequently, film with blown out highlights
is not going to produce any information in the blown out
areas. No data, no results. Dimming these are not going
to produce valid data. If the exposure is not within
the linear region of the medium, then all information
outside of this will be lost (or very difficult to retrieve,
if at all). The factor here is D, or density. This is
usually a big deal issue with scanners. One would like the
highest Dmax possible. It is a log function. Dmax of
4 is quite good. Cheap scanners are 3.2 or thereabout at
best.

Bad digital pix are bad either due to over or under-exposure.
Same for film. The difference is that most users seem to
use 8-bit images. Some even cling to JPEG! The best, IMO,
way to do this is to deal only in 16-bit TIFF images as a
final result. For intermediary files, use RAW (like Nikon NEF).
Then, use Optipix or Bibble to expand these to full value.
Once optimized, they can be reduced to 8-bits. This also holds
true for SEM capture. 16-bit is best. Then process and finally
reduce to 8-bits for public consumption. This of course assumes
that your data capture hardware does more than 8-bits native.
If not, you have only 256 shades of grey to work with. Not good.
Your highlights will be gone and there will be no detail in the
shadows (dark areas).

gary g.



At 12:53 PM 3/29/2005, you wrote:

} Is this truly so?
}
} That CCDs have a wider dynamic range than film?
}
} My amateurish and limited experience had led me to the conclusion that,
} with digital
} images, dimming overexposed images, or brightening underexposed ones,
doesn't
} reveal any hoped-for detail, unlike film.
}
} But maybe this is somehow a consequence of the capture and subsequent
} processing
} that goes on between the CCD and my monitor screen.
}
} cheers
}
} rtch
}
}
}
} Date sent: Mon, 28 Mar 2005 22:20:19 -0800
} To: Microscopy-at-microscopy.com
} } From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: [Microscopy] Re: 18% gray by digital cameras
}
} }
} }
} } ----------------------------------------------------------------------
} } -------- The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help






From MicroscopyL-request-at-ns.microscopy.com Tue Mar 29 22:55:11 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 29 Mar 2005 21:07:40 -0800
Subject: [Microscopy] RE: Re: 18% gray by digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

At 08:24 PM 3/29/2005, you wrote:
} [snip]
} I wanted to have everyone use the RAW image format which does the least or
} no in-camera processing, but mine is the only one that will allow that.
} BTW, the Nikon NEF is 16 bit per channel (on a D1X) and can be opened in
} Photoshop CS by using the OPEN AS command and selecting the correct format,
} doing as much of the processing as can be done before converting to 8-bit.
} I guess that in one respect film is the same as digital in that if there is
} no data, there is nothing you can do about it. Well, maybe with film you
} can if you know you under or overexposed you can try to adjust development
} time and temp. I'm not sure there is anything you can do with the output of
} a CCD chip.

Yes....you can with RAW. Take the image and look at it visually
and histogram. If it is not a good image, fix the situation
and re-shoot. If in doubt, underexpose by 1/3 to 2/3 stop.
Fix the image after the fact in Photoshop using Optipix
or Fovea or Bibble. Definitely do not over expose.

This is the dilemma--no information, no results. What
this means is that if you have a highlight, if it is overexposed,
it will be blown out--all white--255 (8-bits). The problem
is that there is very little wiggle room with 8-bits.
Thus, use 16-bits (12- or 14-bits actual). Then adjust
downwards after touching up the picture. This also applies
to the dark areas. So Dmax plays into this. Back to the
Zone System.

Using the D1x, the options are Bibble, Nikon Capture and
Optipix. They all work but specially in their own
respects. The D1x at 6M pixels becomes 10.5M pixels in
Bibble, et. al. Much (some) of the CCD is discarded
for normal output. This lost area is recovered by
Bibble, et. al.

gary g.



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 02:48:05 2005



From: Lyn Waterhouse :      lyn.waterhouse-at-adelaide.edu.au
Date: Wed, 30 Mar 2005 18:30:49 +0930
Subject: [Microscopy] ruthenium tetroxide waste

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
Does anyone know if ruthenium tetroxide waste can be neutralised in the
same way as osmium tetroxide - ie, with vegetable oil? I would have
thought this would be the right way, as do others I've spoken to, but
no-one has known for sure. I noticed that the info with the Ru says that
it reacts violently with ethanol, whereas Os doesn't, and I wondered if
there are other differences in how it should be handled to make it safe.
Thanks.
Regards,
Lyn

Lyn Waterhouse
Electron Microscopist
Adelaide Microscopy
University of Adelaide
Adelaide SA 5005
Ph: (08) 8303 4074 or 8303 5855
Fax: (08) 8303 4356
http://www.adelaide.edu.au/microscopy


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 08:32:09 2005



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 30 Mar 2005 15:41:18 +0100
Subject: [Microscopy] JEOL840 - EMP quest.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

As I was trying to explain a collegue some points of the SEM's optic,
I stumbled over the EMP mode avaible on the JSM 840, and recommended to
aligne the gun.

If I have good understood, the signal obtained is the variation in
intensity of the beam emitted by the filament-wehnelt in the different
directions. The (upper ?) alignement coils scan to explore these emission
directions, the normal scanning coils being off, and the sample being only
a "SE/BE emitter". But the shift and tilt functions are the result of a
combination of the upper and lower alignement coils. So, how can I work
with, and together use them to aligne themself !

Does someone know more, how does this work ? How are the alignements
coils conected in normal observation mode, and in EMP mode ?

By the way, I never used this mode to aligne the beam. I've always done
that on the image, looking for the maximum brightness. And the settings
were always different (and as good or better) from these with the EMP mode
!

But now, (not too late), I want to understand...

Thanks

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 08:38:33 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 30 Mar 2005 09:52:10 -0500
Subject: [Microscopy] ruthenium tetroxide waste

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lyn,

What is this about osmium tetroxide being neutralized with vegetable oil?! I've
never heard that...could you elaborate? I'm intrigued.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: Lyn Waterhouse [mailto:lyn.waterhouse-at-adelaide.edu.au]
Sent: Wednesday, March 30, 2005 4:01 AM
To: Microscopy-at-microscopy.com

Hi all,
Does anyone know if ruthenium tetroxide waste can be neutralised in the
same way as osmium tetroxide - ie, with vegetable oil? I would have
thought this would be the right way, as do others I've spoken to, but
no-one has known for sure. I noticed that the info with the Ru says that
it reacts violently with ethanol, whereas Os doesn't, and I wondered if
there are other differences in how it should be handled to make it safe.
Thanks.
Regards,
Lyn

Lyn Waterhouse
Electron Microscopist
Adelaide Microscopy
University of Adelaide
Adelaide SA 5005
Ph: (08) 8303 4074 or 8303 5855
Fax: (08) 8303 4356
http://www.adelaide.edu.au/microscopy




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 08:39:02 2005



From: fulton.2-at-osu.edu
Date: Wed, 30 Mar 2005 09:51:36 -0500
Subject: [Microscopy] Brightness problem with Hitachi H-7500 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To any labs out there using a Hitachi H-7500 TEM on biological samples:

We use an H-7500 primarily to image things like organelles and /or viruses.
We installed a Lab6 filament several months ago to overcome
inadequate brightness at mags of ~ 100KX.
There was a minor improvement in brightness, but not nearly enough.
We have an old Philips EM201 TEM which greatly outperforms the
Hitachi in terms of contrast and brightness at high mag.
Has anyone had similar problems?
Were you able to correct this situation?
If so we would greatly appreciate your suggestions.


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 14:32:48 2005



From: David Burk :      dburk-at-lsu.edu
Date: Wed, 30 Mar 2005 14:45:37 -0600
Subject: [Microscopy] Help with pollen image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear microscopy/image analysis experts,
 
I was approached today by a student who needs to count pollen grains on
slides.  The pollen has been stained with fuschin and is quite easy to see. 
The problem is that she needs to count all the grains on over 100 slides (2
samples/slide).  There must be some way to automate the counting procedure –
please let there be a way.  I have, at my disposal, ImageJ and Image Pro
Plus.  I have tried to manipulate the images so that the standard “count
particles” would do exactly that – count the particles.  Unfortunately, the
grains tend to clump together and this gives the software (so far) fits in
that it can’t discern the edges of the pollen grains.  I would really like
to help this person out as I can remember how much I enjoyed counting
trichome branch points for my own work on many many leaves.  If anyone out
there has any previous experience in manipulating images for use in any of
the mentioned programs, I would greatly appreciate your input.  I have
placed a sample image (1MB) on our website if you would like to see what I
have to work with.  I’d also like to point out that what she needs is a
“semi-accurate” count.  Missing 1% of the grains (or adding phantom grains)
won’t be considered bad.  I will personally advise the student to
acknowledge the person (or persons) who help us in this matter AND I promise
that I will toast your name at the next social gathering I attend and dance
a jig in your honor.
 
The sample image can be found at this address:
 
http://www.biology.lsu.edu/facilities/micro_fac/downloads.htm
 
Thanks for your help!
 
David H. Burk
Socolofsky Microscopy Center
Department of Biological Sciences
Louisiana State University





From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 16:38:53 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Wed, 30 Mar 2005 15:50:14 -0700
Subject: [Microscopy] Help with pollen image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello David,

there is a solution that might work for you. I can only speak for our own
software, but perhaps there are similar functions in the software that you
have. In our software, analySIS, we have modules for grain boundary
reconstruction. This typically works by using an algorithm to reconstruct
grain boundaries. Of course, to the software it doesn't matter if it is
grains in metals or pollen, so you can apply the module to separate the
touching pollen. After that it is a pretty simple application of basic image
analysis to threshold the pollen and count and measure them. You could then
further refine the measurement by looking only for circular "particles".

To show you how that looks, I have taken the image you put on your web site,
and applied the technique. I put the result on our ftp site
(ftp.soft-imaging.com) (go to the pub/outgoing folder). It is called
"pollentest_analySIS.tif".

Again, I don't know how you would do this in the software that you have, but
perhaps one of the users of that software could help you translate the
process.

since this is only "semi-help" as I can't tell you how to do this with the
software you have, it's OK if you don't do the jig ;-)

Mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: David Burk [mailto:dburk-at-lsu.edu]
Sent: Wednesday, March 30, 2005 13:46
To: microscopy-at-msa.microscopy.com

Dear microscopy/image analysis experts,
 
I was approached today by a student who needs to count pollen grains on
slides.  The pollen has been stained with fuschin and is quite easy to see. 
The problem is that she needs to count all the grains on over 100 slides (2
samples/slide).  There must be some way to automate the counting procedure -
please let there be a way.  I have, at my disposal, ImageJ and Image Pro
Plus.  I have tried to manipulate the images so that the standard "count
particles" would do exactly that - count the particles.  Unfortunately, the
grains tend to clump together and this gives the software (so far) fits in
that it can't discern the edges of the pollen grains.  I would really like
to help this person out as I can remember how much I enjoyed counting
trichome branch points for my own work on many many leaves.  If anyone out
there has any previous experience in manipulating images for use in any of
the mentioned programs, I would greatly appreciate your input.  I have
placed a sample image (1MB) on our website if you would like to see what I
have to work with.  I'd also like to point out that what she needs is a
"semi-accurate" count.  Missing 1% of the grains (or adding phantom grains)
won't be considered bad.  I will personally advise the student to
acknowledge the person (or persons) who help us in this matter AND I promise
that I will toast your name at the next social gathering I attend and dance
a jig in your honor.
 
The sample image can be found at this address:
 
http://www.biology.lsu.edu/facilities/micro_fac/downloads.htm
 
Thanks for your help!
 
David H. Burk
Socolofsky Microscopy Center
Department of Biological Sciences
Louisiana State University







From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 16:49:17 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Wed, 30 Mar 2005 18:01:02 +0200
Subject: [Microscopy] Re: Help with pollen image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

David,
In your image, the grains look to be a
uniform size. So if you can threshold
successfully to separate grains from everything
else, you can measure the total area occupied by
grains (clustered or otherwise). THen divide by
the average area per grain.

Tobias



} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \
University of Massachusetts
/ / / \ \ \
Amherst, MA, 01003
/ / ___ / \ \__/ \ ____ Voice: 413 - 545 - 1533
Fax: 413 - 545 - 3243
http://www.bio.umass.edu/biology/baskin/



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 16:49:07 2005



From: dwa1-at-lehigh.edu (by way of MicroscopyListserver)
Date: Thu, 31 Mar 2005 09:01:55 +1000
Subject: [Microscopy] viaWWW: Coates and Welter SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (dwa1-at-lehigh.edu) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, March 30, 2005 at 13:58:39
---------------------------------------------------------------------------

Email: dwa1-at-lehigh.edu
Name: Dave Ackland

Organization: lehigh university

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I have been asked to find out information on a scanning
electron microscope made by Coates and Welter.
Does anyone have any information on Coates and Welter cwik-scan
elctron microscopes.
Manuals, circuit diagrams, instruction manuals ECT.
any information would be of great help.

Thanks Dave Ackland



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 18:15:32 2005



From: SGKCCK-at-aol.com (by way of MicroscopyListserver)
Date: Thu, 31 Mar 2005 10:05:49 +1000
Subject: [Microscopy] viaWWW: Sample Preparation Workshops

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
} Diatome U.S., Electron Microscopy Sciences, and Leica Microsystems is
} pleased to announce two new Sample Preparation Workshops:
}
} 1. Materials Science
} 2. Cryo-Techniques/ImmunoGold Labeling
}
} The Materials Science workshop which takes place on April 28th- May 1, 2005
} will take you from Specimen Preparation and Ultramicrotomy through Imaging.
} You will have the opportunity to learn the theories and practice materials
} microscopy and ultramicrotomy.
}
} The curriculum will cover a multitude of samples(Hard as well as Polymers)
} and will include sample preparation, polymer staining, embedding,
} ultramicrotomy, Sample surfacing for SEM, AFM and others as well as
} much more.
} Participants will be allowed to bring there own samples and there
} will be adequate
} time for hands on experience.
}
} The Faculty will include renowned men in the Materials field including Dr.
} Tom Malis from Canada, Helmut Gnaegi from Switzerland, and Bob
} Vastenhout from
} the Netherlands.
}
} The Cryo-Technique/ImmunoLabeling workshop which takes place on May 1- May
} 4th, 2005 will cover theory and practice of cryo-techniques for biological
} sample preparation and immunogold labeling techniques. The curriculum will
} cover the theories underlying immunogold labeling protocols,
} specimen preparation
} techniques and sectioning, silver enhancement of gold particles and much
} more. Participants will be given adequate time for hands on practice.
}
} The Faculty will include Peter van de Plas from the Netherlands, Helmut
} Gnaegi from Switzerland, and Dr. Paul Verkade from Germany.
}
} Both courses are limited to 15 participants and there is only a few
} openings left which are going fast. If you are interested please
} Call or Email us
} at the below address or visit our web site and click the downloadable PDF
} application for the workshop found on the EMS Home page.
}
} Stacie Kirsch
} Electron Microscopy Sciences/Diatome U.S.
}
} Ann Korsen
} Leica Microsystems
}
} For more information:
}
} Tel: 215-412-8402
} Fax: 215-412-8452
}
} E-Mail: _sgkcck-at-aol.com_
} Web Site: _www.emsdiasum.com_
}


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 18:27:43 2005



From: DrJohnRuss-at-aol.com
Date: Wed, 30 Mar 2005 19:39:56 EST
Subject: [Microscopy] Re: Help with pollen image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There are a couple of ways to skin this cat. With Image Pro Plus you could go
the "real counting" route and threshold the image and then separate the
features with a watershed and then count them. An even more accurate way would be
to cross correlate the image with a dark circle about the size of a grain and
count the spikes in the resulting image, which will be completely separated and
easy to threshold. But for a VERY fast semiquantitative way to get the number
of grains, why not select a small but representative number (say 10-20) of
individual grains and carefully measure the average size of a single grain. Then
just take the total thresholded area of all grains, divide by the mean single
grain size, and use that as the number of grains per slide.

John Russ
=======
In a message dated 3/30/05 4:40:14 PM, dburk-at-lsu.edu writes:

} I was approached today by a student who needs to count pollen grains on
}
} slides.  The pollen has been stained with fuschin and is quite easy to
} see. 
}
} The problem is that she needs to count all the grains on over 100 slides
} (2
}
} samples/slide).  There must be some way to automate the counting procedure
} –
}
} please let there be a way.  I have, at my disposal, ImageJ and Image Pro
}
} Plus.  I have tried to manipulate the images so that the standard “count
}
} particles†would do exactly that – count the particles.  Unfortunately,
} the
}
} grains tend to clump together and this gives the software (so far) fits
} in
}
} that it can’t discern the edges of the pollen grains.  I would really like
}
} to help this person out as I can remember how much I enjoyed counting
}
} trichome branch points for my own work on many many leaves.  If anyone
} out
}
} there has any previous experience in manipulating images for use in any
} of
}
} the mentioned programs, I would greatly appreciate your input.  I have
}
} placed a sample image (1MB) on our website if you would like to see what
} I
}
} have to work with.  I’d also like to point out that what she needs is a
}
} “semi-accurate†count.  Missing 1% of the grains (or adding phantom grains)
}
} won’t be considered bad.  I will personally advise the student to
}
} acknowledge the person (or persons) who help us in this matter AND I promise
}
} that I will toast your name at the next social gathering I attend and dance
}
} a jig in your honor.



From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 19:05:01 2005



From: vfink-at-shaw.ca (by way of MicroscopyListserver)
Date: Thu, 31 Mar 2005 11:17:46 +1000
Subject: [Microscopy] viaWWW: Used SEM/TEM/XRD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (vfink-at-shaw.ca) from
http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, March 30, 2005 at 18:36:07
---------------------------------------------------------------------------

Email: vfink-at-shaw.ca
Name: Victoria

Title-Subject: [Microscopy] [Filtered] MListserver: Looking for used SEM and TEM

Question: Dear All,

We are looking for used, and functioning SEM, TEM, and XRD for the
research center. Any information, or suggestions are highly
appreciated.
Can you recommend us a good source of used/ with reduced prices
equipment for the starting up microscopy Lab?

Victoria
Hydrenx, Inc.
IL, Chicago



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 19:57:16 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 30 Mar 2005 18:25:11 -0800
Subject: [Microscopy] Re: RE: ruthenium tetroxide waste

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 30, 2005, at 6:52 AM, Sherwood, Margaret wrote:

} What is this about osmium tetroxide being neutralized with vegetable
} oil?! I've
} never heard that...could you elaborate? I'm intrigued.
}
Dear Peggy,
OsO4 adds across C-C double bonds (perhaps I should have written C=C),
so unsaturated fats, such as corn oil, will react covalently with OsO4.
After that reaction, no OsO4 vapor will be released into the lab.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Mar 30 21:48:39 2005



From: Larry Allard :      allardlfjr-at-ornl.gov
Date: Wed, 30 Mar 2005 23:03:19 -0500
Subject: [Microscopy] Update on "interesting specimen prep..."

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers:

There have obviously been a lot of replies, both on and off line, to
my query; thanks again to all. I've replied to everyone (I hope),
and as soon as my carpal tunnel syndrome is cured, I'll get busy and
try some of the tricks! ;-)
This clearly is a good example of one of the most valuable aspects of
the microscopy listserver, and I appreciate all the input...

Larry
--
Dr. Lawrence F. Allard
Distinguished Research Staff Member
High Temperature Materials Laboratory
Microscopy, Microanalysis, Microstructures Group
Metals and Ceramics Division
Oak Ridge National Laboratory
1 Bethel Valley Road
PO Box 2008
Oak Ridge, TN 37831-6064
(note: the last 4 lines are sufficient for mailing or overnight
courier service)
865-574-4981
865-576-5413 Fax
http://www.ms.ornl.gov/htmlhome/mauc


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 02:50:01 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 31 Mar 2005 04:02:59 -0500
Subject: [Microscopy] osmium tetroxide "neutralization"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Bill Tivol wrote:
===================================================
OsO4 adds across C-C double bonds (perhaps I should have written C=C), so
unsaturated fats, such as corn oil, will react covalently with OsO4.
After that reaction, no OsO4 vapor will be released into the lab.
====================================================
We have been working on the development of an osmium recycling kit. We
have some out on beta test presently. Unfortunately, it is not something
that will be commercialized in the immediate future.

When osmium tetroxide is mixed with any kind of oil, corn or otherwise, the
osmium metal for all practical purposes, at least with today's technology,
is rendered economically non-recyclable.

But the other methods that have been published for reducing any existing
tetroxide down to the dioxide leave the reduced osmium in a form that under
certain conditions, assuming large enough volumes, can be economically
recycled. Getting rid of the arsenic in the spent buffers is one of the
stumbling blocks.

Since osmium is a non-renewable resource, this kind of thinking might be
worthy of consideration. It might also be worth keeping not one but two
bottles of "osmium for recycling", one for the spent buffers without arsenic
and the other for spent buffer with arsenic. The day might come where the
one could be recycled much more easily than the other.

Chuck
===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 09:57:13 2005



From: Alexander Solodukhin :      as4j-at-virginia.edu
Date: Thu, 31 Mar 2005 11:09:52 -0500
Subject: [Microscopy] Postdoc_Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Department of Anesthesiology, University of Virginia. A Research Associate
position is available with Drs. Sando, Solodukhin & Kretsinger for analysis
of structure and activation of protein kinase C (PKC) isozymes at a membrane
surface.

The candidate should have a Ph.D. with experience in protein purification,
enzyme assays and protein structure or membrane biophysics. Experience in
the PKC field and/or in analysis of 2D crystals is an advantage.

Send CV and three letters of recommendation to

Dr. J. Sando
Department of Anesthesiology
University of Virginia Health System
P.O. Box 800710, Charlottesville
VA 22908-0710
jjs-at-virginia.edu.

Position open until filled. The University of Virginia is an Equal
Opportunity/Affirmative Action Employer.




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 09:58:53 2005



From: Jan Factor :      jfactor-at-ns.purchase.edu
Date: Thu, 31 Mar 2005 11:14:25 -0500
Subject: [Microscopy] Re: Re: Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Once again, I have found the discussion on the list interesting and
informative.
Getting past the technical details of isotope decay, etc., I am
interested in (concerned about) care required in handling and using
uranyl acetate in the EM lab. We dispose of used uranyl acetate
separately from other hazardous waste, and it goes out as radioactive
waste. In the lab, we take the same routine precautions as with other
toxins (gloves, etc.), but our radiation safety officer has said that no
special shielding of the stock or disposal bottles is needed, nor
extraordinary precautions.
I wonder if one of the knowledgeable correspondents to this
discussion could share the latest thinking about safety and handling
precautions in the lab.
--Jan Factor

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College, State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



Bill Tivol wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
}
} On Mar 21, 2005, at 5:24 PM, Beaurega wrote:
}
} } If you hold your 'pancake' geiger counter or equivalent near DU, it will
} } not make single clicks like background cosmic rays do. It will emit a
} } continuous tone on the times one scale even through a glass bottle
} } and its
} } surrounding metal can. I had access to pounds of the stuff gathered
} } over
} } the years by a chemist that I worked with in that wet lab. The gamma
} } radiation was detectable 12 feet away through walls and locked metal
} } cabinets. So inhalling DU or UA is bad.
} }
} Dear Beauriga,
} What one reads from UAc at the distances you mention is, indeed,
} gamma radiation, which is emitted from some of the isotopes in the U
} decay chains. There will be a (nearly) steady state established where
} each isotope in each decay chain (different for U-238 and U-235) has
} the appropriate number of atoms such that the activities of each
} isotope are the same; i.e., for each isotope, one atom is produced by
} the parent isotope(s) for every one that decays. It is the gammas
} that can cause damage at long distances, but each alpha which enters a
} cell--thus acting over distances shorter that its very short range,
} less than the thickness of the dead layer of the skin--does much more
} damage that the gammas.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 10:37:40 2005



From: Kevin Ryan :      kevin-at-mediacy.com
Date: Thu, 31 Mar 2005 11:49:22 -0500
Subject: [Microscopy] RE: Help with pollen image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here's how to use Image-Pro (that's what I'm familiar with, as I write
it) to obtain a fairly accurate count;

- Set your intensity threshold to separate the grains from the
background. Using a grayscale version of your image (to avoid color
info, as the image doesn't have much color) I set this to the range
0-93.
- Set up Count/Size to measure "Area" and "Cluster".
- Count the objects, run a watershed split to separate those that can be
split.
- Examine the statistics to see the sum value of "Cluster", which uses
area measurements to estimate clumps of objects. This method takes the
histogram of object areas, finds the first peak, and uses that as an
estimate of the mean size of a singleton object. Larger objects are then
measured and their cluster value set to the integer scale of their area
to the singleton area.

I get a value in the 450's on this image, using the filtering in the
next paragraph. Your milage may vary, but that's probably a reasonable
estimate. I'm afraid I haven't hand-counted to compare this with a
visual result. That's highly recommended for checking - run a visual
count on a representative image or two, compare with the automated
result described above, and you can estimate a bias correction factor.

I would suggest setting some filters on minimum size and possibly on
mean intensity. Eliminating objects less than half the area of your
grains will give a better estimate of the mean size of single grains,
and there is a large dark area on the bottom with a mean of 38, as
opposed to the the grains which have a mean of ~80. So, setting the
filters to an area of 20-1000000, and mean density of 50-255, I obtained
a cluster sum of 456 grains.

I hope that's helpful!

-- Kevin Ryan
kevin-at-mediacy.com


} -----Original Message-----
} From: David Burk [mailto:dburk-at-lsu.edu]
} Sent: Wednesday, March 30, 2005 3:46 PM
} To: microscopy-at-msa.microscopy.com
} Subject: [Microscopy] Help with pollen image analysis
}
}
}
}
} --------------------------------------------------------------
} ----------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} -----------------
}
} Dear microscopy/image analysis experts,
}  
} I was approached today by a student who needs to count pollen
} grains on slides.  The pollen has been stained with fuschin
} and is quite easy to see. 
} The problem is that she needs to count all the grains on over
} 100 slides (2 samples/slide).  There must be some way to
} automate the counting procedure – please let there be a way. 
} I have, at my disposal, ImageJ and Image Pro Plus.  I have
} tried to manipulate the images so that the standard “count
} particles” would do exactly that – count the particles. 
} Unfortunately, the grains tend to clump together and this
} gives the software (so far) fits in that it can’t discern the
} edges of the pollen grains.  I would really like to help this
} person out as I can remember how much I enjoyed counting
} trichome branch points for my own work on many many leaves. 
} If anyone out there has any previous experience in
} manipulating images for use in any of the mentioned programs,
} I would greatly appreciate your input.  I have placed a
} sample image (1MB) on our website if you would like to see
} what I have to work with.  I’d also like to point out that
} what she needs is a “semi-accurate” count.  Missing 1% of the
} grains (or adding phantom grains) won’t be considered bad.  I
} will personally advise the student to acknowledge the person
} (or persons) who help us in this matter AND I promise that I
} will toast your name at the next social gathering I attend
} and dance a jig in your honor.
}  
} The sample image can be found at this address:
}  
} http://www.biology.lsu.edu/facilities/micro_fac/downloads.htm
}  
} Thanks for your help!
}  
} David H. Burk
} Socolofsky Microscopy Center
} Department of Biological Sciences
} Louisiana State University
}
}
}
}




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 14:05:06 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 31 Mar 2005 15:17:57 -0500
Subject: [Microscopy] Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have had a request for information on women who were on the forefront
during the early days of development of electron microscopy. Of most
interest would be those who were involved in Biological imaging. I drew a
blank as all the early pioneers that came to mind were men.

Help me out here!

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 14:24:20 2005



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 31 Mar 2005 12:31:58 -0800
Subject: [Microscopy] nanowires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

Any advice regarding looking at 'nanowires'?

Someone asked me to do this, and I don't think it is working too well, but
they are determined to forge ahead and keep looking, even though I don't
see much.

I am using our good old conventional SEM that works fine for our usual
applications, coated biological samples.

These wires are said to be made of silicon, are very small, and reside on
little circuits that may not be coated.

I think this customer should look elsewhere for an FESEM and look at the
uncoated samples at low KV, or cough up a few bucks so we can buy one.

Have you tried to look at nanowires and what was the best stragtegy?

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 15:09:02 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Thu, 31 Mar 2005 13:36:52 -0800
Subject: [Microscopy] Re: Re: Re: viaWWW: Uranyl acetate problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mar 31, 2005, at 8:14 AM, Jan Factor wrote:

} Getting past the technical details of isotope decay, etc., I am
} interested in (concerned about) care required in handling and using
} uranyl acetate in the EM lab. We dispose of used uranyl acetate
} separately from other hazardous waste, and it goes out as radioactive
} waste. In the lab, we take the same routine precautions as with other
} toxins (gloves, etc.), but our radiation safety officer has said that
} no special shielding of the stock or disposal bottles is needed, nor
} extraordinary precautions.
} I wonder if one of the knowledgeable correspondents to this
} discussion could share the latest thinking about safety and handling
} precautions in the lab.
}
Dear Jan,
Those are the procedures we follow also, with the additional step that
all UAc is used and disposed of in a specified area of a hood. We also
wash our hands after finishing staining and cleanup as an extra
precaution against ingestion.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 15:43:11 2005



From: Libby Shaw :      elshaw-at-MIT.EDU
Date: Thu, 31 Mar 2005 16:54:56 -0500
Subject: [Microscopy] Re: Update on "interesting specimen prep..."

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Larry:

I haven't seen any answers to your query on the listserver. Could you
share the responses? (When the CTS permits, of course) We've got someone
here with a very similar sample prep problem.

Thanks,

Libby Shaw

} Date: Wed, 30 Mar 2005 23:03:19 -0500
} From: Larry Allard {allardlfjr-at-ornl.gov}
} Subject: [Microscopy] Update on "interesting specimen prep..."
} To: microscopy-at-msa.microscopy.com
}
} Listers:
}
} There have obviously been a lot of replies, both on and off line, to
} my query; thanks again to all. I've replied to everyone (I hope),
} and as soon as my carpal tunnel syndrome is cured, I'll get busy and
} try some of the tricks! ;-)
} This clearly is a good example of one of the most valuable aspects of
} the microscopy listserver, and I appreciate all the input...
}
} Larry
} --
} Dr. Lawrence F. Allard
} Distinguished Research Staff Member
} High Temperature Materials Laboratory
} Microscopy, Microanalysis, Microstructures Group
} Metals and Ceramics Division
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} PO Box 2008
} Oak Ridge, TN 37831-6064
} (note: the last 4 lines are sufficient for mailing or overnight
} courier service)
} 865-574-4981
} 865-576-5413 Fax
} http://www.ms.ornl.gov/htmlhome/mauc
}
****************************************************************
Elisabeth L. Shaw, Facility Coordinator
Surface and Spectroscopy Labs
Analytical Shared Experimental Facilities
MIT Center for Materials Science and Engineering

Address: MIT Room 13-4149 Tel: 617-253-5045
77 Massachusetts Avenue Email: elshaw-at-mit.edu
Cambridge, MA 02139 Fax: 617-258-6478
http://web.mit.edu/cmse/www/
****************************************************************


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 15:50:24 2005



From: David Burk :      dburk-at-lsu.edu
Date: Thu, 31 Mar 2005 16:03:07 -0600
Subject: [Microscopy] Help with pollen grains -- THANKS!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

I just wanted to thank everyone (if I haven't already) for taking the time
to offer advice, suggestions, and even do some work for me in solving the
"pollen count dilemma". I will try out your suggestions just as soon as I
get some time to do so! I think the simplest solution involves thresholding
the image and simply dividing the total area by the area of a single grain
while the most "creative" involved hiring an undergraduate for $5.15/hour to
count by hand.

I really appreciate your help and I hope the student appreciates it as much
as me.

Again, thanks to all of you for your help!

David H. Burk
Socolofsky Microscopy Center
Department of Biological Sciences
Louisiana State University



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 16:11:21 2005



From: Paul Webster :      pwebster-at-hei.org
Date: Thu, 31 Mar 2005 14:21:58 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debbie,

Don't forget Elizabeth Luduc, who worked with Bernard trying to cut frozen
sections in a cold room in Paris (1960's I think). As far as I know she is
working in Mew England somewhere.

Their contribution to the development of cryosectioning is very significant.
They inpired Professor Christensen to develop the first cryo-chamber for an
ultramicrotome.

Regards,

Paul Webster.


On 3/31/05 12:17 PM, "Debby Sherman" {dsherman-at-purdue.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} I have had a request for information on women who were on the forefront
} during the early days of development of electron microscopy. Of most
} interest would be those who were involved in Biological imaging. I drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 16:29:50 2005



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 31 Mar 2005 14:43:02 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

How early is early? 1950s? The wonderful protozoologist who gave me
my first EM job at U.C. Berkeley in '53, Dorothy Pitelka. I watched
her make glass knives by throwing a piece of plate glass on the floor
& picking thru the shards. And certainly Audrey Glauert, who is
still with us; ask her for more names: amg44-at-cam.ac.uk

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 16:32:36 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 31 Mar 2005 17:44:16 -0500
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How about Marilyn Farquhar, who used to work
here at Yale with another great pioneer of
cell biology and EM - George Palade. She
published some influential EM papers
in the 50's already.

Marc

On Thursday, March 31, 2005, at 03:17 PM, Debby Sherman wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} I have had a request for information on women who were on the forefront
} during the early days of development of electron microscopy. Of most
} interest would be those who were involved in Biological imaging. I
} drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 17:44:40 2005



From: Rosemary White :      Rosemary.White-at-csiro.au
Date: Fri, 01 Apr 2005 09:58:05 +1000
Subject: [Microscopy] Re: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If you only want info on the engineering side of EM development, I'd guess
they'll all(?) be blokes, but there were definitely some top female
biologists who used EM in the early days - Katherine Esau and Irene Manton
immediately come to mind.
And Jean Hanson (E.J. Hanson) who first showed the structure of actin
microfilaments.
cheers,
Rosemary


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 18:00:16 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Thu, 31 Mar 2005 16:12:33 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debby,

Also Norma Reid
bob.norma-at-bopis.co.nz

and
Chris Pella for contacts of who was there
contact at Ted Pella Inc.

Good Luck
There were actually quite a few women involved.

Judy

Debby Sherman wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 19:05:07 2005



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 31 Mar 2005 17:22:47 -0800
Subject: [Microscopy] Re: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Not so. Gertrude (Trudy) Rempfer was a major designer (electrostatic
TEMs), but she always worked in industry & couldn't publish. See
MSA's tape (now DVD) #149, made when she won MSA's Distinguished
Scientist award.

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 20:44:00 2005



From: Angela V. Klaus :      avklaus-at-amnh.org
Date: Thu, 31 Mar 2005 21:55:48 -0500 (EST)
Subject: [Microscopy] Re: Re: Re: Women pioneers in electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gertrude Rempfer is still working in EM. She's Emerita at Portland State
University (Physics Dept) and a recent co-investigator on an NSF
instrument development award for continuing work on photoelectron
microscopy (PEM).


}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-796-5977
Fax: 212-496-3480



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 21:08:47 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Thu, 31 Mar 2005 21:28:55 -0600
Subject: [Microscopy] Re: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

debby et al

from the biology side, it is good to see names such as glauert. but
have we already forgotten june almeida, francis doane and nan anderson.
they certainly did their bit for microbiology, especially virology.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 21:36:14 2005



From: Rosemary White :      Rosemary.White-at-csiro.au
Date: Fri, 01 Apr 2005 13:49:19 +1000
Subject: [Microscopy] Re: Re: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm happy to stand corrected!

} From: Caroline Schooley {schooley-at-mcn.org}
} Date: Thu, 31 Mar 2005 17:22:47 -0800
} To: Rosemary White {Rosemary.White-at-csiro.au}
} Cc: Microscopy-at-MSA.Microscopy.Com
} Subject: [Microscopy] Re: Re: Women pioneers in electron microscopy
}
} }
-----------------------------------------------------------------------------} }
-
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------------
} } --
} }
} } If you only want info on the engineering side of EM development, I'd guess
} } they'll all(?) be blokes,
}
} Not so. Gertrude (Trudy) Rempfer was a major designer (electrostatic
} TEMs), but she always worked in industry & couldn't publish. See
} MSA's tape (now DVD) #149, made when she won MSA's Distinguished
} Scientist award.
}
} Caroline
} --
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
} Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html
}


From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 22:33:35 2005



From: alicia thompson :      athompso-at-usc.edu
Date: Thu, 31 Mar 2005 20:45:17 -0800
Subject: [Microscopy] Re: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How about Miriam Salpeter at Cornell University. She was the first with fine grain EM autoradiography in the mid 60s.
Alicia Thompson



From MicroscopyL-request-at-ns.microscopy.com Thu Mar 31 23:27:01 2005



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Fri, 1 Apr 2005 07:45:10 +0200
Subject: [Microscopy] [ Microscopy] EMU design lessons learned from the past

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All
I just love this list. We are having a workshop on Laboratory infrastructure and M {management.
Most EM Units (like ours) I know was shoved into a existing building. Some even of 2nd and 3rd floors.
I will appreciate it if people can share there experience with relationship to performance problems observed, instrument involved (W, LaB6 or FEG, SEM, ESEM, Duelbeem TEM etc.) How it was solved and a few nice and humorous examples will be great. I hope to get the point across that an EM unit is a important part of a University, and if possible must be taken into consideration during building design and ultimately must play a part in the location decision of a University.

Thanks


Since some mail do get Lost, Bounces, etc Please send a duplicate/copy of all urgent mail to:

coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}

Mr S. H. Coetzee
Electron Microscope Unit
University of Botswana
Private Bag 0022
Gaborone
Botswana
Phone : +267 355 2462/5222
Mobile : +267 718 36547
Fax : +267 318 5097
e-mail : coetzees-at-mopipi.ub.bw



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 08:52:04 2005



From: Beth Richardson :      beth-at-plantbio.uga.edu
Date: Fri, 1 Apr 2005 10:04:06 -0500
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On my bulletin board I have a wonder photo of Katherine Esau - she is
standing next to a TEM (column) and the caption says "Modern American
Gothic".

On Thursday, March 31, 2005, at 03:17 PM, Debby Sherman wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} I have had a request for information on women who were on the forefront
} during the early days of development of electron microscopy. Of most
} interest would be those who were involved in Biological imaging. I
} drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
}
**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 09:38:40 2005



From: Scheltens Frank J Contr AFRL/MLLN :      Frank.Scheltens-at-wpafb.af.mil
Date: Fri, 1 Apr 2005 10:28:19 -0500
Subject: [Microscopy] Electron Microscopy Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopy Technician


UES Incorporated, a high-tech R&D company in Dayton, Ohio and operator of the AFRL Materials Characterization Facility (MCF), has an immediate need for a full time electron microcopy research technician. The candidate must have a minimum of three to five years experience, a High School degree (Associates degree preferred) and be capable of providing on-site support at the MCF located within WPAFB AFRL/Materials and Manufacturing Directorate. The ideal candidate will be experienced in the operation and maintenance of TEM, SEM and associated support equipment as well as the preparation of materials for examination by these techniques. Experience with the electron microscopy of high temperature ceramic, intermetallic, metallic, metal matrix composite and ceramic matrix composite materials, as well as their preparation by metallographic, electropolishing and ion milling techniques is highly desirable. Knowledge of polymer, Carbon / Carbon composite and semi-conductor electron m!
icroscopy and the preparation of these materials, including experience in microtomy of materials, is preferred. The technician must also be capable of training and assisting individuals in the execution of all of the above techniques. Additionally, they will be responsible for the maintenance of the electron microscopes, which are all under service contract, and the maintenance of associated support equipment such as sample preparation facilities, as well as other general laboratory duties. U.S. Citizenship is required.


UES, Inc. Attn: Debbie Yount 4401 Dayton-Xenia Road Dayton, OH 45432 Fax: (937) 426-0533; E-mail: humanresources-at-ues.com; AA/EEO Employer





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 10:49:25 2005



From: Ronald Smith :      rsmith-at-uwo.ca
Date: Fri, 01 Apr 2005 12:05:46 -0500
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debby,
Just a couple of Canadians that come to mind are Dr.Bessie Borwein,
Dept. of Anatomy University of Western Ontario, Dr Elaine Humphrey
University of British Columbia, vancouver B.C. Dr. Margaret McCully,
Carlton University, Ottawa.

Ron.
Debby Sherman wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Ronald J. Smith
Department of Biology
Room 235, Biological & Geological Sciences Bldg.
U.W.O., London, Ontario
N6A 5B7
(519) 661-2111 ext.86486




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 11:10:44 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Fri, 01 Apr 2005 12:19:51 -0500
Subject: [Microscopy] Re: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This would make a great dissertation topic for a History of Science
student. MSA should consider funding such a student to go around and
interview these women who are still alive and close associates of those who
have gone on to the great EM lab in the sky.

At 10:04 AM 4/1/2005, Beth Richardson wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 11:19:14 2005



From: Salamon, Daniel :      Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Fri, 1 Apr 2005 12:31:59 -0500
Subject: [Microscopy] checking for sample contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

It seems that people are putting all the wrong samples into our field emission SEM and it's causing contamination problems. We decided to ask all people to fill in forms with detailed information about their samples, but it's hard to predict how all samples will behave in vacuum.

1. Can you suggest a way to check that samples are vacuum-friendly before loading them into the SEM?

2. Is there any good way to periodically clean the specimen chamber from contamination?

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-081 ECERF Bldg
9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 11:43:46 2005



From: BETTY FABER :      bfaber-at-lsc.org
Date: Fri, 01 Apr 2005 12:54:46 -0500
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Eleanor H. Slifer

She did insect chemoreceptors.

Academy of Natural Sciences in Philadelphia


cheers

Betty Faber
Liberty Science center

} } } Beth Richardson {beth-at-plantbio.uga.edu} - 4/1/05 10:04 AM } } }


------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

On my bulletin board I have a wonder photo of Katherine Esau - she is

standing next to a TEM (column) and the caption says "Modern American

Gothic".

On Thursday, March 31, 2005, at 03:17 PM, Debby Sherman wrote:

}
}
}
-----------------------------------------------------------------------

} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------

} --------
}
} I have had a request for information on women who were on the
forefront
} during the early days of development of electron microscopy. Of
most
} interest would be those who were involved in Biological imaging. I
} drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
}
**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************

***





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 13:20:30 2005



From: Ted Pella :      ted_pella-at-tedpella.com
Date: Fri, 1 Apr 2005 11:33:32 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Debbie and Caroline,

I can't remember the lady who, in the early days, worked at Rockefeller
and married Dr. George Palade. It seemed to me that she was very active
in the early research days. She finished her career at the Pathology
Department at the Univ. of Calif. Medical School in San Francisco. I'll
try to recall that name.

I agree that Audrey Glauert is a terrific choice.

Ted Pella



-----Original Message-----
} From: Caroline Schooley [mailto:schooley-at-mcn.org]
Sent: Thursday, March 31, 2005 2:43 PM
To: Debby Sherman
Cc: Microscopy-at-MSA.Microscopy.Com

} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} during the early days of development of electron microscopy. Of most
} interest would be those who were involved in Biological imaging. I drew

} a blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby -

How early is early? 1950s? The wonderful protozoologist who gave me
my first EM job at U.C. Berkeley in '53, Dorothy Pitelka. I watched
her make glass knives by throwing a piece of plate glass on the floor
& picking thru the shards. And certainly Audrey Glauert, who is
still with us; ask her for more names: amg44-at-cam.ac.uk

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates:
http://www.fortbragg.k12.ca.us/AG/marinelab.html






From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 13:22:33 2005



From: Ted Pella :      ted_pella-at-tedpella.com
Date: Fri, 1 Apr 2005 11:35:38 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How could I forget!! Talk about lapses.

Chris and I visited with Norma Reid (who wrote "Sectioning" in the
Glauert series)in February, in New Zealand - and with her husband Bob
Hudson.

As far as I am concerned, Judy and Caroline are qualified for the
incredible teaching, scrimping and saving under tough budgeting
conditions while teaching a large number of individuals over years.
And, in article submissions.




-----Original Message-----
} From: Judy Murphy [mailto:murphyjudy-at-comcast.net]
Sent: Thursday, March 31, 2005 4:13 PM
To: Debby Sherman; microscopy-at-msa.microscopy.com

Hi Debby,

Also Norma Reid
bob.norma-at-bopis.co.nz

and
Chris Pella for contacts of who was there
contact at Ted Pella Inc.

Good Luck
There were actually quite a few women involved.

Judy

Debby Sherman wrote:

} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} during the early days of development of electron microscopy. Of most
} interest would be those who were involved in Biological imaging. I drew

} a blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
}
}
}
}
}
}






From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 14:02:34 2005



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 1 Apr 2005 10:14:47 -1000 (HST)
Subject: [Microscopy] Re: checking for sample contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi-

I just dealt with this, probably as you were hitting the "send" button.
Someone came in with charcoal that they said probably had 20% humidity. We
have a Hitachi FESEM with a pre-pump chamber/airlock and if something
takes longer than 30 sec to pump, it's too wet or gassy. So I pumped it in
the sputter coater to 30 mTorr for a few minutes, then put it in the
pre-pump chamber for a bit, then there was no problem.

For contamination, I question people closely about their samples. Covered
in oil? No way. Some things I'll sneak a quick peek at to see what
happens. Stuff embedded in epoxy and then polished, I'll warn them not to
let the beam touch the epoxy. Preview anything suspicious and ban it if
it's going to muck up the column!

} It seems that people are putting all the wrong samples into our field emission SEM and it's causing contamination problems. We decided to ask all people to fill in forms with detailed information about their samples, but it's hard to predict how all samples will behave in vacuum.
}
} 1. Can you suggest a way to check that samples are vacuum-friendly before loading them into the SEM?
}
} 2. Is there any good way to periodically clean the specimen chamber from contamination?

Good luck,
Tina


****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 15:35:33 2005



From: Ted Pella :      ted_pella-at-tedpella.com
Date: Fri, 1 Apr 2005 13:48:41 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yes Debbie, Marilyn Farquhar was the woman I was thinking of - married
to Dr. Geo. Palade, Rockefeller-Yale-UCSFMedSch

Ted

-----Original Message-----
} From: Marc Pypaert [mailto:marc.pypaert-at-yale.edu]
Sent: Thursday, March 31, 2005 2:44 PM
To: Debby Sherman
Cc: message to: MSA list

How about Marilyn Farquhar, who used to work
here at Yale with another great pioneer of
cell biology and EM - George Palade. She
published some influential EM papers
in the 50's already.

Marc

On Thursday, March 31, 2005, at 03:17 PM, Debby Sherman wrote:

}
}
} ----------------------------------------------------------------------
} -
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------
} --------
}
} I have had a request for information on women who were on the
} forefront during the early days of development of electron microscopy.

} Of most interest would be those who were involved in Biological
imaging. I
} drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446







From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 16:19:15 2005



From: Sandra Masur :      sandra.masur-at-mssm.edu
Date: Fri, 01 Apr 2005 17:28:12 -0500
Subject: [Microscopy] Re: RE: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marilyn Farquhar
and she is still active at UCSD - her career is not finished!

On Friday, April 1, 2005, at 02:33 PM, Ted Pella wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Greetings Debbie and Caroline,
}
} I can't remember the lady who, in the early days, worked at Rockefeller
} and married Dr. George Palade. It seemed to me that she was very
} active
} in the early research days. She finished her career at the Pathology
} Department at the Univ. of Calif. Medical School in San Francisco.
} I'll
} try to recall that name.
}
} I agree that Audrey Glauert is a terrific choice.
}
} Ted Pella
}
}
}
} -----Original Message-----
} } From: Caroline Schooley [mailto:schooley-at-mcn.org]
} Sent: Thursday, March 31, 2005 2:43 PM
} To: Debby Sherman
} Cc: Microscopy-at-MSA.Microscopy.Com
} Subject: [Microscopy] Re: Women pioneers in electron microscopy
}
}
}
}
} -----------------------------------------------------------------------
} -
} ------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} -
} -------
}
} } ----------------------------------------------------------------------
} } -
} } -------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } -
} --------
} }
} } I have had a request for information on women who were on the
} } forefront
}
} } during the early days of development of electron microscopy. Of most
} } interest would be those who were involved in Biological imaging. I
} } drew
}
} } a blank as all the early pioneers that came to mind were men.
} }
} } Help me out here!
} }
} } Debby -
}
} How early is early? 1950s? The wonderful protozoologist who gave me
} my first EM job at U.C. Berkeley in '53, Dorothy Pitelka. I watched
} her make glass knives by throwing a piece of plate glass on the floor
} & picking thru the shards. And certainly Audrey Glauert, who is
} still with us; ask her for more names: amg44-at-cam.ac.uk
}
} Caroline
} --
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
} Intertidal invertebrates:
} http://www.fortbragg.k12.ca.us/AG/marinelab.html
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 17:01:38 2005



From: Webster, Paul :      PWebster-at-hei.org
Date: Fri, 1 Apr 2005 15:12:55 -0800
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ted,

A small correction is needed here. Professor Farquar is still based in San Diego (UCSD), where she moved to when she left Yale.

No-one has mentioned Betty Hay yet. She ran the Anatomy Department at Harvard Medical School for many years. This department was very influential in sponsoring electron microscopy for biologists. I can think of a few names that have links with this department (Fawcett, Ito, Karnovski). I am sure there are more in this list. I think I once saw a photo of a young Ted Pella looking down an AEI microscope that may have been in Harvard.

Is it true that the early TEMs used rubber mallets to align the apertures?

Paul Webster.

-----Original Message-----
} From: Ted Pella [mailto:ted_pella-at-tedpella.com]
Sent: Fri 4/1/2005 1:48 PM
To: 'Marc Pypaert'; 'Debby Sherman'
Cc: 'message to: MSA list'

Yes Debbie, Marilyn Farquhar was the woman I was thinking of - married
to Dr. Geo. Palade, Rockefeller-Yale-UCSFMedSch

Ted

-----Original Message-----
} From: Marc Pypaert [mailto:marc.pypaert-at-yale.edu]
Sent: Thursday, March 31, 2005 2:44 PM
To: Debby Sherman
Cc: message to: MSA list

How about Marilyn Farquhar, who used to work
here at Yale with another great pioneer of
cell biology and EM - George Palade. She
published some influential EM papers
in the 50's already.

Marc

On Thursday, March 31, 2005, at 03:17 PM, Debby Sherman wrote:

}
}
} ----------------------------------------------------------------------
} -
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------
} --------
}
} I have had a request for information on women who were on the
} forefront during the early days of development of electron microscopy.

} Of most interest would be those who were involved in Biological
imaging. I
} drew a
} blank as all the early pioneers that came to mind were men.
}
} Help me out here!
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446












From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 18:35:18 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Fri, 01 Apr 2005 18:47:40 -0600
Subject: [Microscopy] Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Karnovsky was in the Pathology dept not Anatomy. I once asked Sandford
Palay (Anatomy at HMS) in the late 80's about using TEM's in the "old" days
and he told me how they used rubber mallets to align the apertures. they
just tapped on the column until they fell into the correct location. I am
not sure but I think it was Fawcett who hired Ito and Hay and then Betty
Hay went on to assume Fawcett's position when he retired.





At 05:12 PM 04/01/05, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 18:42:51 2005



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 1 Apr 2005 17:00:01 -0800
Subject: [Microscopy] RE: RE: Re: Women pioneers in electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Paul -

I guess I AM a pioneer, because I've seen this. Dorothy Pitelka used
Tom Hayes' RCA EMU 2 at what is now Lawrence Berkeley Lab in the mid
50s. That scope had a "fixed" aperture & owners loosened the
clamping screws. Tom used a ball peen hammer - gently.

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 19:37:51 2005



From: Narasimhan S :      narasimhanpotti-at-hotmail.com
Date: Sat, 02 Apr 2005 01:50:36 +0000
Subject: [Microscopy] Star Anaphase & Chromosome stickiness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Can anybody tell me what is chromosome stickiness and star anaphase ?
How do they occur ?

thanks all,
Narasimhan

_________________________________________________________________
The MSN Survey!
http://www.cross-tab.com/surveys/run/test.asp?sid=2026&respid=1 Help us help
you better!



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 19:51:06 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Fri, 01 Apr 2005 18:02:52 -0800
Subject: [Microscopy] Re: RE: RE: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We stigmated (objective lens) our RCA 2A and 2E with a rubber mallet for
yrs - very gently!!! It moved the aperture around enough to affect the
astigmatism.

We had the 2nd Cambridge SEM in the country Mark I and did several
things with that with a rubber mallet!!!!!

Judy Murphy



Caroline Schooley wrote:

}
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} }
} } Is it true that the early TEMs used rubber mallets to align the
} } apertures?
} }
} } Paul Webster.
}
}
} Paul -
}
} I guess I AM a pioneer, because I've seen this. Dorothy Pitelka used
} Tom Hayes' RCA EMU 2 at what is now Lawrence Berkeley Lab in the mid
} 50s. That scope had a "fixed" aperture & owners loosened the clamping
} screws. Tom used a ball peen hammer - gently.
}
} Caroline



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 1 21:57:59 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Fri, 01 Apr 2005 22:16:54 -0600
Subject: [Microscopy] Re: RE: RE: Re: Women pioneers in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
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paul

unlike carolyn, my first experience was with an old Siemens, they used a
soft brass mallet, not a ball peen hammer. i say they - a young
technician trainee would never have been allowed to touch the beast.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Sat Apr 2 00:50:13 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Fri, 01 Apr 2005 23:02:27 -0800
Subject: [Microscopy] Re: Re: RE: RE: Re: Women pioneers in electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Siemens I: Like Paul's lab, most weren't allowed to tinker with the
Siemens I as it was the "research microscope" in the early 60's at the
Bevier Hall Center for Microscopy at Univ of Illinois (Urbana). ALL of
us users however were many times tempted as The bathroom was a couple
doors away down the hall. EVERYTIME someone flushed the toilet, the
Siemens "red barred" which meant it got a signal for low water pressure,
and went through its vacuum sequencing and one had to manually valve it
back to high vacuum which took a long time. It wasn't very conducive
for "research microscopy" or any kind of microscopy on busy lab days
(i.e. when lots of folks used the bathroom).

Can't resist sharing this story also about the Siemens: apologize for
the aside ahead of time.
The Siemens was in a room (column only, power supplies in separate room)
about 4 ft x 5 ft or very small. The Electronics Engineer in the lab
decided one morning to bring his son's 10 ft boa constrictor into lab
(haven't a clue why, but anyway he did) and decided he wanted it
contained in a small room i.e. the Siemens room (smallest room in the
set of labs). He had come in at 5:30am and put it in the room. The
snake of course liked the warmth near the diffusion pump at the front
bottom of the column. I had the Siemens that day from 6am to 6pm. Got
the sample loaded, and turned out the lights (the snake being out of
sight underneath). Well you can imagine my surprise when something
crawled around my legs which were under the scope. After running out of
the room, and likely screaming at the top of my voice, the Siemens took
on a whole different meaning besides microscopy!!!!! Later, much
later,it was determined that the engineer knew I had the scope at 6, and
thus planned to have it in there when I arrived. ALSO, I didn't
appreciate the humor in it all until a GREAT deal of time passed! AND
yeah, before anyone asks, after that I didn't like snakes much,
especially crawling on me.

Judy



Judy Murphy, PhD
Microscopy Training, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net


paul r hazelton wrote:

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From MicroscopyL-request-at-ns.microscopy.com Sat Apr 2 19:24:26 2005



From: Corvos-at-aol.com
Date: Sun, 3 Apr 2005 17:51:27 EDT
Subject: [Microscopy] Vacuum Pump service Houston TX area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, I guess I have to add the name of Elizabeth MacLean, who was my
guide in a course in Optical Methods in Biology at Brandeis during
1963. I remember not only the optical theory, but also the aroma of
floating colloidion films out of amyl acetate.

Send reply to: {ted_pella-at-tedpella.com}
} From: "Ted Pella" {ted_pella-at-tedpella.com}
To: "'Caroline Schooley'" {schooley-at-mcn.org} ,
"'Debby Sherman'" {dsherman-at-purdue.edu}
Copies to: {Microscopy-at-MSA.Microscopy.Com}

All,

Does anyone know of a Edwards vacuum pump service company in the Houston TX
area.

Regards,

Walter Protheroe
E-MAC, Inc
corvos-at-aol.com
www.emaci.com



From MicroscopyL-request-at-ns.microscopy.com Sun Apr 3 21:38:13 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Sun, 03 Apr 2005 21:52:22 -0500
Subject: [Microscopy] Women EM Pioneers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My request for names of women who were pioneers in the field of electron
microscopy has resulted in a extensive list. The list starts with those
active in the 40¹s and continues on to many women who are still very active
in the field today. Their inclusion in the list is due to their influence
on someone who took the time to honor them by responding to my initial
request.

I have sorted out those that I knew were active in the very early days.
These women were indeed pioneers in both scientific research, when the
numbers of active women were rather small, and in the new discipline of
electron microscopy. In addition there is an extensive list of others who
carried on with the research using EM and helped expand or open new areas of
research.

Thanks to all who replied and I apologize if I left someone off of the list.
It was not intentional but rather a matter of trying to do to many things in
too short of an amount of time.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


Women in the early 40¹s
Beatrice Deacon and Alice Gray (Toronto, E.F Burton group)
Katherine Polevitsky (Bacteriology, U. of Penn.)
Irene Manton FRS: very early plant electron microscopist who worked
primarily at Leeds University in the UK. She held the Chair of Botany there
from 1946 - 1969, and died in 1988. A building at Leeds University is now
named after her as well as prize from the Linnean Society and Phycological
Society



mid to late-40s
Mary Schuster Jaffe: formvar fenestrated films (first worked with E.F.
Fullam)
Gertrude Fleming Rempfer: Electron optics -- one of the best, man or woman

Early 50¹s
Jean Hanson (E.J. Hanson) who first showed the structure of actin
microfilaments.
Marie Jakus: Biological EM (collagen & muscle proteins) with F.O. Schmitt
and Cecil Hall at MIT
Audrey Glauert: originally trained at the Rockefeller Cytology Group
(Porter and Palade) along with Farquar and Bonnneville
Marilyn Farquhar (Yale)
Mary Bonneville


EMSA Education Committee members in the early years (with most still active)
Caroline Schooley
Judy Murphy
Betty Mathews
Beverly Giammara

June Almeida, Francis Doane and Nan Anderson: Microbiology, esp virology

Katherine Esau: light microscopist who entered the field of EM to better
elucidate plant structure, and plant pathogen/host systems. Member of Nat¹l
Academy of Science

Elizabeth (Betsy) Haye: Head of Anatomy, Harvard School of Medicine

Dorothy Pitelka: protobiologist..Berkeley

Chris Pella

Helen Padykula (Mt. Holyoke 1948, Harvard 1954) the American Society of Cell
Biology¹s second woman president

Geraldine Gauthier, a student of Helen Padykula, was at Brown, Harvard,
Wellesley and U. Mass. Med. from the 60s to the 80s.
Norma Reid: wrote "Sectioning" in the Glauert series

Dr. Betty Geren Uzman, Mary Jaffe, and Marie Jakus.

Eleanor H. Slifer: insect chemoreceptors. Academy of Natural Sciences in
Philadelphia

Elizabeth Luduc,cro-TEM sectioning methods (1960s)

Agnes Oberlin: electron microscopist in France (TEM) going back to the 40s.
carbon-based materials

Helen Gay: first of cold Spring Harbor and later The
University of Michigan? In the mid 1950's she published beautiful
micrographs of cell nuclei and nucleocytoplasmic relationships --"blebs".





From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:46:03 2005



From: somayyeh_kheiri-at-yahoo.com (by way of Ask-A-Microscopist)
Date: Mon, 4 Apr 2005 09:02:10 -0500
Subject: [Microscopy] AskAMicroscopist: FAA preserved materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, April 3, 2005 at 00:13:14
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh kheiri

Organization: urmia university

Education: Graduate College

Location: Urmia,west azarbaijan,Iran

Question: Hello Dear all

I have a question about FAA preserved materials:
I am doing experiments on ssectionig fruit pericarp and leaves.I have gathered the material in FAA
how long do you put the material in FAA?the epidermis in the fruits that i have preserved in FAA sometimes become detached from the mesocarp.
I thought may this problem be because of the long time of preservation i have kept the material.(6month)I thought the tissues have been loosened.

can it be the reason?

thank you very much
Somayyeh



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:47:53 2005



From: Bstud-at-yandex.ru (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:03:55 -0500
Subject: [Microscopy] viaWWW: Backscattered and secondary electrons

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Bstud-at-yandex.ru) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 4, 2005 at 04:09:17
---------------------------------------------------------------------------

Email: Bstud-at-yandex.ru
Name: Andrey

Organization: IPM RAS

Title-Subject: [Microscopy] [Filtered] Backscattered and secondary electrons

Question: I'm doing some work in electron-beam lithografy and need to know: how to calculate, where backscattered and secondary electrons will occur. Please give me references to abstracts or computer programs which may be helpful for this.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:48:13 2005



From: Somayyeh_kheiri-at-yahoo.com (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:04:23 -0500
Subject: [Microscopy] viaWWW: pollen grain counting

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Somayyeh_kheiri-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Sunday, April 3, 2005 at 06:26:26
---------------------------------------------------------------------------

Email: Somayyeh_kheiri-at-yahoo.com
Name: Somayyeh

Organization: urmia university

Title-Subject: [Microscopy] [Filtered] about pollen grain counting

Question: Hello dear all

I have been counting the number of pollen grains and ovules to get the pollen/ovule ratio for verbascum species
and populations.

I pressed the anthers of the flowers and provided a susponsion of the debris of anthers in distilled water to count the pollen
grains in a 5 microliter volume.

some of the samples in the susponsion were counted
one day after they were provided so the result showed
differences from the first sample which was counted immidiately after providing the susponsion.
I myself thought keeping the material in water for oneday
caused the grains to be attached together and the amount of the grains that are counted are reduced.

can anybody help me solve the problem?

is this reasonable or there maybe errors in the method of counting?

I appreciate any kind reply.

Somayyeh



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:50:14 2005



From: vfink-at-shaw.ca (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:06:26 -0500
Subject: [Microscopy] viaWWW: Thanks to all for recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (vfink-at-shaw.ca) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, April 1, 2005 at 17:37:07
---------------------------------------------------------------------------

Email: vfink-at-shaw.ca
Name: Victoria

Title-Subject: [Microscopy] [Filtered] Thanks to all for recommendations

Question: I just wanted to thank you all for the making recommendations, and providing with useful information regarding to the used analytic techniques search.
It is amazing how helpful could be this professional collaboration networking!

Victoria

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:50:21 2005



From: narasimhanpotti-at-hotmmail.com (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:05:55 -0500
Subject: [Microscopy] viaWWW: chromosome stickiness & star anaphase

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (narasimhanpotti-at-hotmmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, April 1, 2005 at 19:40:04
---------------------------------------------------------------------------

Email: narasimhanpotti-at-hotmmail.com
Name: Narasimhan

Organization: University of Kerala

Title-Subject: [Microscopy] [Filtered] chromosome stickiness & star anaphase

Question: Dear all,

Can anbody tell me what is chromosome stickiness and star anaphase ? How do they occur in mitosis or meiosis ?

thanks,
Narasimhan

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 08:50:50 2005



From: corvos-at-aol.com (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 09:06:50 -0500
Subject: [Microscopy] viaWWW: Roughing pumps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (corvos-at-aol.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, April 1, 2005 at 17:02:43
---------------------------------------------------------------------------

Email: corvos-at-aol.com
Name: Walter J. Protheroe Jr.

Organization: Energy - Micro-Analytical Consultants, Inc.

Title-Subject: [Microscopy] [Filtered] MListserver: Roughing pumps

Question: I am looking for a company in the Houston, TX area that can rebuild Edwards roughing pumps. Any info would be a help...

Regards,

Walter Protheroe
E-MAC, Inc
corvos-at-aol.com
www.emaci.com

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 10:10:25 2005



From: glaevsky-at-ECE.NEU.EDU
Date: Mon, 04 Apr 2005 11:26:31 -0400
Subject: [Microscopy] Motorized Linear Positioner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

I'm looking for a motorized linear positioner that will be putting
different types of mirrors in and out of the beam path. X resolution is
not critical. Speed is a moderate concern. I need an approximate travel
distance of 16 cm. Weight is also not a concern.

I searched and found about 30 different places. Does anyone have any
experience with any product such as this?

Thanks in advance for your help.
Best,

Gary



Gary Laevsky, Ph.D.
Keck Facility Manager, CenSSIS
Northeastern University
302 Stearns
360 Huntington Ave.
Boston, MA 02115
voice(617) 373 - 2589 {br}
fax(617) 373 - 7783 {br} {br}

http://www.censsis.neu.edu

http://www.ece.neu.edu/groups/osl

http://www.keck3dfm.neu.edu



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 17:07:47 2005



From: GAFTUNE-at-A0L.COM (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 17:23:35 -0500
Subject: [Microscopy] viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (GAFTUNE-at-A0L.COM) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 4, 2005 at 12:14:11
---------------------------------------------------------------------------

Email: GAFTUNE-at-A0L.COM
Name: GERALD FISHER

Organization: NA

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I AM AN ENGINEER WHO IS INTERESTED IN THE TECHNIQUES USED INIDENTIFYING PARTICLES FILTERED ONTO FILTER PAPER USING AN OPTICAL MICROSCOPE. THE PARTICL SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON NOMINAL DIAMETER OR LENGTH.

THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING SOLUTIONS. THEY MIGHT TYPICALLY BE ACTIVATED CARBON, METAL FINES(LIKE ELEMENTAL NICKEL OR COPPER), FILTER MEDIA POWDERS, OR ATMOSPHERIC DIRT.

ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE QUANTITY OF EACH PRESENT?

CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 17:08:10 2005



From: diller-at-assisi.de (by way of MicroscopyListserver)
Date: Mon, 4 Apr 2005 17:24:05 -0500
Subject: [Microscopy] viaWWW: EMSCOPE Sputtercoater SC 500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-assisi.de) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 4, 2005 at 13:52:55
---------------------------------------------------------------------------

Email: diller-at-assisi.de
Name: Stefan Diller

Organization: Photography

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear listeners,
I am looking for manuals and electronic layouts for an EMSCOPE
Sputtercoater SC 500 with carbon coater head SB 250.
Any help would be fine, especially a short explanation how to work with it...
I reach the first trip-point at 0,15 Torr but then nothing happens after pressing the start-button...

Best regards,
Stefan Diller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 18:17:53 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 05 Apr 2005 11:33:57 +1200
Subject: [Microscopy] EDS detector vacuum lifetime?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How long should the vacuum (and thus the LN2 consumption and the resolution) of a
Be-window EDS detector last?

The LN2 consumption of ours rose to about 2.2 litres/day by the time it was two years
old, then it was sent back to the factory for a leak-check and a pump 'n' bake.

When we got it back, the consumption rate was about 1.3 litres/day, but after a further
twenty-two months, the consumption had risen to around 2 litres.day. During this time
the detector had been mounted on an SEM which was under vacuum 24/7, and had not
been allowed to warm up at all.

The consumption then continued to rise, and after a further six months, all under
vacuum and with no warmups, it ran out of LN2 over 3 1/2 days of Easter, indicating a
consumption rate of about 2.2 litres/day.

And now, after recooling, the consumtion is up to about 2.4 litres/day, and, of course,
the resolution has degraded by about 3 eV.

It seems to me that this detector has always had a slow leak, not through the window,
which the factory failed to find and fix both at the time of manufacture and when it was
returned to them.

The factory, however, maintains that, because it was leak-checked when it was
returned to them, that any problem must have developed subsequently.

Now I'm not a deeply cynical person, but it seems to me unlikely that twice the same
detector has developed vacuum problems within two years of leaving the factory.

I will be interested in the opinions of others about the history of this particular detector,
and also in the experience of others with the vacuum lifetimes of their EDS detectors.

cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 18:54:09 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Mon, 4 Apr 2005 18:07:49 -0600
Subject: [Microscopy] viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gerald,

please contact me via email. We have a product for exactly that application.

Mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: GAFTUNE-at-A0L.COM [mailto:GAFTUNE-at-A0L.COM]
Sent: Monday, April 04, 2005 16:24
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (GAFTUNE-at-A0L.COM) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April
4, 2005 at 12:14:11
---------------------------------------------------------------------------

Email: GAFTUNE-at-A0L.COM
Name: GERALD FISHER

Organization: NA

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I AM AN ENGINEER WHO IS INTERESTED IN THE TECHNIQUES USED
INIDENTIFYING PARTICLES FILTERED ONTO FILTER PAPER USING AN OPTICAL
MICROSCOPE. THE PARTICL SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON
NOMINAL DIAMETER OR LENGTH.

THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING SOLUTIONS. THEY MIGHT
TYPICALLY BE ACTIVATED CARBON, METAL FINES(LIKE ELEMENTAL NICKEL OR COPPER),
FILTER MEDIA POWDERS, OR ATMOSPHERIC DIRT.

ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE QUANTITY OF EACH
PRESENT?

CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 20:16:37 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 4 Apr 2005 18:47:45 -0700
Subject: [Microscopy] Re: EDS detector vacuum lifetime?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 4, 2005, at 4:33 PM, Ritchie Sims wrote:

} How long should the vacuum (and thus the LN2 consumption and the
} resolution) of a
} Be-window EDS detector last?
}
} The LN2 consumption of ours rose to about 2.2 litres/day by the time
} it was two years
} old, then it was sent back to the factory for a leak-check and a pump
} 'n' bake.
}
} When we got it back, the consumption rate was about 1.3 litres/day,
} but after a further
} twenty-two months, the consumption had risen to around 2 litres.day.
} During this time
} the detector had been mounted on an SEM which was under vacuum 24/7,
} and had not
} been allowed to warm up at all.
}
} The consumption then continued to rise, and after a further six
} months, all under
} vacuum and with no warmups, it ran out of LN2 over 3 1/2 days of
} Easter, indicating a
} consumption rate of about 2.2 litres/day.
}
} And now, after recooling, the consumtion is up to about 2.4
} litres/day, and, of course,
} the resolution has degraded by about 3 eV.
}
} It seems to me that this detector has always had a slow leak, not
} through the window,
} which the factory failed to find and fix both at the time of
} manufacture and when it was
} returned to them.
}
} The factory, however, maintains that, because it was leak-checked when
} it was
} returned to them, that any problem must have developed subsequently.
}
} Now I'm not a deeply cynical person, but it seems to me unlikely that
} twice the same
} detector has developed vacuum problems within two years of leaving the
} factory.
}
} I will be interested in the opinions of others about the history of
} this particular detector,
} and also in the experience of others with the vacuum lifetimes of
} their EDS detectors.
}
Dear Rich,
The detector we had on the HVEM also had a slow leak, and I think that
any system that has o-rings, valves, and other parts that can be
disassembled will have a slow leak. When we mounted a modified back
plate on our system to facilitate pump-outs in situ, we had to fiddle
with it to minimize the leaks, and, even so, we had to pump the system
out every few months to get good resolution. (BTW, 3 eV is very
good--I suspect you mean 300 eV.) We didn't ever have to replace the
LN2 more frequently than once a week, but your dewar may be smaller
than ours. 2+ l/d is a fast consumption rate, though. If you can hook
up a leak detector to the EDS detector, you might be able to find the
leak(s) and remedy it or them. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 20:21:31 2005



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Tue, 5 Apr 2005 11:36:20 +1000
Subject: [Microscopy] Re: [ Microscopy] EMU design lessons learned from the past

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When we moved into our new building, a home had to be found for the
TEM. The new building, though beautiful and heritage listed sandstone,
was not exactly suited for an electron microscope. We had the choice of
the first or second floor. There was no choice of the underground
railway or the main road outside. As there was no money to pay for
proper testing, I spent a delightful day checking for vibration with a
saucer of water. I could be found for long periods at prospective
locations crouched on the floor with my saucer staring intently at the
water surface waiting for a train or the traffic lights to change. As
we in a hospital complex, this gave rise to several people solicitously
stopping and asking if I needed medical attention. I dare say they were
completely confused when I explained my mission; I really should have
thought up something more plausible, such as an alien attack!

As it turned out, the microscope is very happy and vibration free in
its second floor location!

The moral? It may seem impossible, but if there's no choice and some
imagination.......


Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
On 1 Apr 2005, at 3:45 PM, Coetzee, Mr S. H Physics Science wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Dear All
} I just love this list. We are having a workshop on Laboratory
} infrastructure and M {management.
} Most EM Units (like ours) I know was shoved into a existing building.
} Some even of 2nd and 3rd floors.
} I will appreciate it if people can share there experience with
} relationship to performance problems observed, instrument involved (W,
} LaB6 or FEG, SEM, ESEM, Duelbeem TEM etc.) How it was solved and a
} few nice and humorous examples will be great. I hope to get the point
} across that an EM unit is a important part of a University, and if
} possible must be taken into consideration during building design and
} ultimately must play a part in the location decision of a University.
}
} Thanks
}
}
} Since some mail do get Lost, Bounces, etc Please send a duplicate/copy
} of all urgent mail to:
}
} coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}
}
} Mr S. H. Coetzee
} Electron Microscope Unit
} University of Botswana
} Private Bag 0022
} Gaborone
} Botswana
} Phone : +267 355 2462/5222
} Mobile : +267 718 36547
} Fax : +267 318 5097
} e-mail : coetzees-at-mopipi.ub.bw
}



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 4 20:44:48 2005



From: moss-at-relia.net
Date: Mon, 4 Apr 2005 19:58:17 -0600 (MDT)
Subject: [Microscopy] Re: viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

SEM with EDX would make this a simple analysis. It could even be automated.

Bill McManus
Mt Ogden Scientific Services
moss-at-relia.net

}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (GAFTUNE-at-A0L.COM) from
} http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
} April 4, 2005 at 12:14:11
} ---------------------------------------------------------------------------
}
} Email: GAFTUNE-at-A0L.COM
} Name: GERALD FISHER
}
} Organization: NA
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: I AM AN ENGINEER WHO IS INTERESTED IN THE TECHNIQUES USED
} INIDENTIFYING PARTICLES FILTERED ONTO FILTER PAPER USING AN OPTICAL
} MICROSCOPE. THE PARTICL SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON
} NOMINAL DIAMETER OR LENGTH.
}
} THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING SOLUTIONS. THEY MIGHT
} TYPICALLY BE ACTIVATED CARBON, METAL FINES(LIKE ELEMENTAL NICKEL OR
} COPPER), FILTER MEDIA POWDERS, OR ATMOSPHERIC DIRT.
}
} ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE QUANTITY OF EACH
} PRESENT?
}
} CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?
}
} ---------------------------------------------------------------------------
}
}



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 02:03:00 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Tue, 05 Apr 2005 02:16:49 -0500
Subject: [Microscopy] Re: viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gerald,

Several thoughts:
If you are having contrast problems, try using mineral oil or immersion oil
to "remove" the paper background. Also, reflected light darkfield
microscopy often helps.

Secondly, regarding counting - if your particles are indeed only 1 micron
in size, it will probably be difficult to get enough pixels to really image
them well with a camera. I'd recommend that you try, but it might be tough
because there won't be enough pixels/particle to really limage them. If
you can, indeed, see them by eye, you may want to invest in a simple grid
that fits in your eyepiece and count the number in a representative number
of squares. If the distribution is homogeneous, you don't have to count
all the particles in all the squares. Just count a representative number
then multiply by the total number of squares.

If the particles are as small as you say they are, you might get tricky and
use a camera to project them onto a computer monitor then make a grid on a
piece of overhead transparency and count them as you see them on the monitor.

These are just several options. I'm sure the listserver will have lots of
others.

Good hunting!

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Visit our website
to learn more about "Optimizing LIght Microscopy". Copies still available
through MME... even for class-room lots ... and we give quantity discounts.


At 05:23 PM 4/4/2005, GAFTUNE-at-A0L.COM wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 04:21:16 2005



From: terry.cooper :      terry.cooper-at-btinternet.com
Date: Tue, 5 Apr 2005 10:35:45 +0100
Subject: [Microscopy] Women pioneers in EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Only picked up my e-mails after a few days but I would agree that Irene
Manton was one of the leaders at least in the UK from the 40's to the 70's.
It so happens that Dr Peter Evennett is giving a talk on the contributions
of Irene Manton (and EM in Leeds) at the 35 year Anniversary Meeting of the
SEMT (Society of Electron Microscope Technology) in The Open University,
Milton Keynes, UK on the 27th of April. If you can get there great as we
would love to see you, but if not I would guess a transcript of the talk
might be available,

Regards

Terry Cooper
TAAB Laboratories Equipment Ltd
3 Minerva House, Calleva Park
Aldermaston, Berks, RG7 8NA, England

Tel ++44(0)118 981 7775
Fax ++44(0)118 981 7881
e-mail sales-at-taab.co.uk
www.taab.co.uk



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 07:59:43 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Tue, 05 Apr 2005 08:13:48 -0500
Subject: [Microscopy] Re: Re: [ Microscopy] EMU design lessons learned

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Diane,
You were very fortunate that you were able to retrofit a facility with
minimum problems to house your microscopes. This is not always possible for
high end instruments and high resolution demands.

One topic to be addressed during the Core Facility Management session at
M&M2005 is construction of a facility for these special needs. I hope all
that are fortunate enough to be able to attend the meeting will come and
share their experiences and solutions to both routine and high end
installation problems during our extensive discussion period.

I hope that the proceedings of this session will be able to be taped and
published in Microscopy Today as we have done previously so all will
benefit.

By the way, this is the first time that this session is officially
sponsored by our newly MSA-certified Focused Interest Group on Facility
Operations.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy




On 4/4/05 8:36 PM, "Diana van Driel" {dianavd-at-eye.usyd.edu.au} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} When we moved into our new building, a home had to be found for the
} TEM. The new building, though beautiful and heritage listed sandstone,
} was not exactly suited for an electron microscope. We had the choice of
} the first or second floor. There was no choice of the underground
} railway or the main road outside. As there was no money to pay for
} proper testing, I spent a delightful day checking for vibration with a
} saucer of water. I could be found for long periods at prospective
} locations crouched on the floor with my saucer staring intently at the
} water surface waiting for a train or the traffic lights to change. As
} we in a hospital complex, this gave rise to several people solicitously
} stopping and asking if I needed medical attention. I dare say they were
} completely confused when I explained my mission; I really should have
} thought up something more plausible, such as an alien attack!
}
} As it turned out, the microscope is very happy and vibration free in
} its second floor location!
}
} The moral? It may seem impossible, but if there's no choice and some
} imagination.......
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney, NSW
} AUSTRALIA 2001
}
} Phone 61 2 93827278
} Mobile 0423 151614
} FAX 61 2 93827318
} On 1 Apr 2005, at 3:45 PM, Coetzee, Mr S. H Physics Science wrote:
}
} }
} }
} } -----------------------------------------------------------------------
} } -------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------
} } --------
} }
} } Dear All
} } I just love this list. We are having a workshop on Laboratory
} } infrastructure and M {management.
} } Most EM Units (like ours) I know was shoved into a existing building.
} } Some even of 2nd and 3rd floors.
} } I will appreciate it if people can share there experience with
} } relationship to performance problems observed, instrument involved (W,
} } LaB6 or FEG, SEM, ESEM, Duelbeem TEM etc.) How it was solved and a
} } few nice and humorous examples will be great. I hope to get the point
} } across that an EM unit is a important part of a University, and if
} } possible must be taken into consideration during building design and
} } ultimately must play a part in the location decision of a University.
} }
} } Thanks
} }
} }
} } Since some mail do get Lost, Bounces, etc Please send a duplicate/copy
} } of all urgent mail to:
} }
} } coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}
} }
} } Mr S. H. Coetzee
} } Electron Microscope Unit
} } University of Botswana
} } Private Bag 0022
} } Gaborone
} } Botswana
} } Phone : +267 355 2462/5222
} } Mobile : +267 718 36547
} } Fax : +267 318 5097
} } e-mail : coetzees-at-mopipi.ub.bw
} }
}
}




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 09:29:05 2005



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Tue, 05 Apr 2005 10:43:28 -0400
Subject: [Microscopy] Floor vibration measurements (was "EMU design lessons...")

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Diana, et al,

Concerning floor vibration.

Perhaps an easier way to use the water saucer trick is to reflect a laser
from the surface onto a wall. By having a large saucer-to-wall distance,
you can amplify the effect of the water ripples. One thing you do need to
watch out for are air currents which can also ripple the surface of the
water. I reduced this effect by taking a large cardboard box (with
appropriate cutouts) and placing it over the water dish.

I used an old HeNe laser we had in the lab, but I suspect that with a
little ingenuity, you could use a laser pointer just as well.

Does anyone else have home-made environmental sensors for EM
labs? (vibration, acoustics, EM fields, etc)?

Cheers,
Henk

At 09:36 PM 04/04/05, Diana van Driel wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all at
once. Lately it doesn't seem to be working.



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 09:37:30 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Tue, 5 Apr 2005 07:52:09 -0700 (PDT)
Subject: [Microscopy] Re: viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gerald:

I agree with the post by Bill McManus that SEM/EDS
methods are the best way to identify the contaminant
sample you described. I do this routinely for various
customers who present contaminants on filter paper. I
don't believe one micron-sized particles are amenable
to analysis using optical microscopy methods. My
approach using SEM/EDS is cutomized for each set of
analyses I perform, depending on the customer's
concerns.

I recommend you get in touch with a laboratory that
has SEM/EDS and an operator that can interpret the
results.

Stu Smalinskas, P.E.
Metallurgist
SKF USA
Plymouth, Michigan
(734) 414-6862

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Gerald Fisher wrote:

Question: I AM AN ENGINEER WHO IS INTERESTED IN THE
TECHNIQUES USED IN IDENTIFYING PARTICLES FILTERED ONTO
FILTER PAPER USING AN OPTICAL MICROSCOPE. THE PARTICLE
SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON NOMINAL
DIAMETER OR LENGTH.

THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING
SOLUTIONS. THEY MIGHT TYPICALLY BE ACTIVATED CARBON,
METAL FINES (LIKE ELEMENTAL NICKEL OR COPPER), FILTER
MEDIA POWDERS, OR ATMOSPHERIC DIRT.

ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE
QUANTITY OF EACH PRESENT?

CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?



__________________________________
Yahoo! Messenger
Show us what our next emoticon should look like. Join the fun.
http://www.advision.webevents.yahoo.com/emoticontest


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 10:30:56 2005



From: Prof. Dr. Wim Van den Broeck :      wim.vandenbroeck-at-UGent.be
Date: Tue, 5 Apr 2005 17:46:12 +0200
Subject: [Microscopy] LM embedding larvae in paraffin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

I need to embed some very small larvae of Ostertagia ostertagi in paraffin,
but they are only visible under the microscope. I have a basic protocol
advising to embed the larvae in 3% agar, fix them overnight in Bouins fluid,
and subsequently process and embed them in paraffin, but does it mean that
the larvae, pre-embedded in agar, can subsequently be fixed, processed and
embedded in paraffin ? Or do I have to remove the agar in a particular step
? Or are there other protocols that I can use ?

Thanks in advance for your comments.

Kind regards,

Wim.


---
Wim Van den Broeck, DVM, MSc, PhD
Docent Cytology and Histology
Department of Morphology,
Faculty of Veterinary Medicine, Ghent University
Salisburylaan 133, B-9820 Merelbeke
BELGIUM
tel.: +32 (0)9 264 77 16
fax: +32 (0)9 264 77 90
Email: wim.vandenbroeck-at-UGent.be
http://allserv.rug.ac.be/~bdepauw/




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 10:39:10 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Tue, 5 Apr 2005 10:54:32 -0500
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to find a suitable scanner to scan Kodak 4489 film, that is:
3.25" X 4" negatives. Preferably as low a priced system as possible would
be suitable, so I'm interested to see what others have done or are doing
when it comes to scanning this size of negative.

I've already noticed that when Nikon or Minolta talks about "electron
microscope film" they have in mind a format of 59mm X 82 mm, which is too
small for us.

Your help with valuable ideas would be GREATLY appreciated in this matter.



Garry Burgess

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg Canada

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 11:22:29 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 05 Apr 2005 09:32:01 -0700
Subject: [Microscopy] Re: Floor vibration measurements (was "EMU design

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I use a small magnetometer that plugs into my notebook PC.
Using Spectrum Laboratory Windows app. Free download is
from here:

www . qsl. net/dl4yhf/spectra1.html

It was made by ham radio folks to recover Morse Code and
to do other radio things but is great for FFT analysis of
all sorts of vibration and acoustic noise.

gary g.
N6OIJ



At 07:43 AM 4/5/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 11:41:20 2005



From: Damian Neuberger :      neuberger1234-at-comcast.net
Date: Tue, 5 Apr 2005 11:56:42 -0500
Subject: [Microscopy] viaWWW: PARTICLES FILTERED ONTO FILTER PAPER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gerald,

There are two components to your proposed analysis: 1) you want to count
particles that are as small as 1 micrometer, and 2) you want to count
particles based on their elemental composition and/or morphology. I am
doubtful that you will be able to clearly distinguish between the groups of
particles using the light microscope and even with EDXS, you may have some
difficulty distinguishing between the activated carbon and some atmospheric
dirt and perhaps the filter media powders depending on their composition
(are they a silicon based material?); the nickel and copper would be easy to
do with EDXS as has been suggested by other replies. The other aspect of
using the light microscope would, as Ms Foster mentioned, be the ability to
image the smallest of the particles with a digital camera, too few pixels.

Another concern is the use of filter paper (depth filters) where the smaller
particles can get trapped within the matrix of the paper and be lost to
imaging on the SEM. What you need to use is a mesh type filtration
membrane. Nuclear track etched membranes can also cause "piling up" of
particles around the pore and consequently you can lose some particles
embedded in a "mountain" of other particles (I've seen this unless you have
very few particles in your solution). If you can get access to an SEM with
EDXS I have another procedure that you can use to filter the particles but
that would require that you 1) can dilute the electroplating solution with
0.45 micrometer or smaller filtered distilled water, 2) that the
electroplating solution will not attack aluminum, and 3) there is no
aluminum or gold in the solution to be filtered.

If you can live with the above limitations, dilute the electroplating
solution with filtered distilled water and filter the particles on a vacuum
evaporated gold coated 0.2 micrometer retention rated Anodisc(TM) filtration
membrane. This filtration medium is like a filter screen so the particles
will all sit atop the flat surface. I've found that coating helps reduce
clumping of the particles and of course reduces charging. The total
particulate burden can be easily determined using a series of Fovea Pro 3.0
(plug-ins for Photoshop) steps which I can give you (other software can
probably do the same thing). The EDXS with other dedicated image analysis
software can also do this for each type of particle based on elemental
composition (probably even automated) although again, separating some of the
types of particles may be problematic.

Damian Neuberger, Ph.D.
2416 Covert Rd
Glenview IL 60025
Tel: (847) 998-8574
email: neuberger1234-at-comcast.net {mailto:neuberger1234-at-comcast.net}


-----Original Message-----
} From: by way of MicroscopyListserver [mailto:GAFTUNE-at-A0L.COM]
Sent: Monday, April 04, 2005 5:24 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (GAFTUNE-at-A0L.COM) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April
4, 2005 at 12:14:11
---------------------------------------------------------------------------

Email: GAFTUNE-at-A0L.COM
Name: GERALD FISHER

Organization: NA

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I AM AN ENGINEER WHO IS INTERESTED IN THE TECHNIQUES USED
INIDENTIFYING PARTICLES FILTERED ONTO FILTER PAPER USING AN OPTICAL
MICROSCOPE. THE PARTICL SIZE WOULD BE MINIMALLY APPROXIMATELY 1 MICRON
NOMINAL DIAMETER OR LENGTH.

THESE PARTICLES WOULD BE PRESENT IN ELECTROPLATING SOLUTIONS. THEY MIGHT
TYPICALLY BE ACTIVATED CARBON, METAL FINES(LIKE ELEMENTAL NICKEL OR COPPER),
FILTER MEDIA POWDERS, OR ATMOSPHERIC DIRT.

ALSO, IS THERE AN ACCOUNTING PROCEDURE TO MEASURE THE QUANTITY OF EACH
PRESENT?

CAN SOMEONE GIVE ME TECHNIQUES AND EQUIPMENT REQUIREMENTS?

---------------------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:06:22 2005



From: Tom Murray :      murraytm-at-u.washington.edu
Date: Tue, 5 Apr 2005 10:21:26 -0700
Subject: [Microscopy] Digital Camera Recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking at moving my Philips EM420 from film to digital imaging.
This is a materials science program so diffraction as well as
bright-field imaging are important.

I have done an online search for suppliers for digital cameras and
found the following:

Gatan
SIS
Teitz
SIA

As with everyone money is a factor in this decision.

I am looking for recommendations in two areas.

1) Do you have experience with cameras from the above manufacturers, or
other manufacturers my search missed? Are you happy with the product
and the support?

2) What are the pros and cons of top mount vs bottom mount cameras?
I know the top mount cameras have imaging areas which are
similar to the plate film my students are used to, but there is lower
resolution. Any other tradeoffs to consider?

Thanks for your help,

Tom

------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email: murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:19:50 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 5 Apr 2005 13:36:58 -0400
Subject: [Microscopy] Re: NESM 22nd Annual Spring Symposium-Woods Hole, MA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The New England Society for Microscopy (NESM), along with it's co-host, the
Connecticut Microscopy Society (CMS)is pleased to announce it's 22nd Annual
Spring Symposium at Marine Biological Lab (MBL) in Woods Hole, MA. The 2-day
Symposium will be held Friday April 29th and Saturday, April 30th. Preceeding
the symposium on Thursday, April 28th, there will be a half-day workshop on
"Presentation Skills and Details for Microscopists" presented by Paul Matsudaira
of The Whitehead Institute-MIT (Cambridge, MA).

The program will include presentations from both the Materials Science and
Biological Sciences. After registration at Noon on Friday, and the welcome by
NESM's President-elect, Dan Gibson, there will be 2 technical sessions which
will include such fascinating talks as large X-ray mapping for the analysis of
geological samples and the use of Northeastern's Keck 3D fusion microscope to
examine protein expression in mouse embryos. At the close of the technical
presentations on Friday, attendees will enjoy socializing at a cocktail hour and
dinner at MBL's Swope Center with a wonderful view of Eel Pond. The
after-dinner speaker, Dr. Greg erickson of Florida Statue University, will
present an interesting talk entitled, "Building Giants: Unlocking the Mysteries
of Dinosaurian Growth Patterns".

The Saturday morning session will include 3 talks, after which the attendees can
meet with our sponsors in the vendor display and peruse the Poster session (many
submitted by students). Monetary prizes will be awarded for the Best Poster,
Best Student Poster, and Best Photo-as-Art. Do come and support our poster
entrants for all their hard work!

Pre-registration is mandatory: if you plan on staying for dinner and if you want
to attend the workshop, you must reserve your spot. Complete details re: the
meeting program, the Poster Session, and the workshop (which is a separate
registration from the Symposium) can be found on NESM's website
(http://nesm.cims.harvard.edu) under "current newsletter".

If you require any information re: the meeting, please contact Paul Bain, NESM's
Treasurer at paul_bain-at-HMS.HARVARD.EDU or by phone: 617-432-3236.

Please plan on joining us at Woods Hole! It is one of the most anticipated
meetings of the year for NESM.

Peggy Sherwood
Corresponding Secretary/Newsletter Editor-NESM











Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:31:19 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Tue, 05 Apr 2005 12:46:16 -0500
Subject: [Microscopy] Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

a key feature in the selection of a scanner for EM film is the Dmax. most
"home" flat bed scanners don't have a sufficiently high enough Dmax value
to capture the essential information. my epson 1680 does a decent job.


} X-Authentication-Warning: ns.microscopy.com: mail set sender to
} MicroscopyL-request-at-ns.microscopy.com using -f
} Date: Tue, 5 Apr 2005 10:54:32 -0500
} From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
} Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives
} To: {microscopy-at-microscopy.com}
} X-Mailer: Internet Mail Service (5.5.2653.19)
} X-OriginalArrivalTime: 05 Apr 2005 17:28:06.0738 (UTC)
} FILETIME=[D461B320:01C53A04]
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:49:36 2005



From: ekomarnicki-at-MacDermid.com
Date: Tue, 5 Apr 2005 14:05:25 -0400
Subject: [Microscopy] Surface roughness using SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does any on the list know if one can perform surface roughness
measurements using a SEM either directly or through EDS software? I am
aware that this can be accomplished using AFMs and light and laser
profilometers, but is there software out there that allows the use of a
SEM?

TIA,

Ed Komarnicki
Sr. Analytical Chemist
MacDermid Inc.



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 12:53:06 2005



From: Yang, Ann-Fook :      YANGA-at-AGR.GC.CA
Date: Tue, 5 Apr 2005 14:03:28 -0400
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am quite happy with Epson Perfection 4870 Photo flatbed scanner (just a happy user).

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Tuesday, April 05, 2005 11:55 AM
To: microscopy-at-microscopy.com


I would like to find a suitable scanner to scan Kodak 4489 film, that is:
3.25" X 4" negatives. Preferably as low a priced system as possible would
be suitable, so I'm interested to see what others have done or are doing
when it comes to scanning this size of negative.

I've already noticed that when Nikon or Minolta talks about "electron
microscope film" they have in mind a format of 59mm X 82 mm, which is too
small for us.

Your help with valuable ideas would be GREATLY appreciated in this matter.



Garry Burgess

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg Canada

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 13:14:08 2005



From: Richard Edelmann :      edelmare-at-MUOhio.Edu
Date: Tue, 5 Apr 2005 13:59:30 -0400
Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Folks:

Help! I need a different name to call "Dualbeam" or "Crossbeam"
instruments since one is trademarked and the other is registered (FEI &
Zeiss).



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 13:26:51 2005



From: Montpetit, Diane :      MontpetitD-at-agr.gc.ca
Date: Tue, 5 Apr 2005 14:40:12 -0400
Subject: [Microscopy] bacteria capsular material and lectin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,



I want to stabilize the capsule surrounding a bacteria in order to see if the lectin I intend to use is binding to a sugar residue located in the capsule itself or on the cell wall.



I have already tried negative staining (PTA) and I was not satisfy with the results. There is some binding but I cannot discriminate clearly if it on the capsule or the wall.



I decided to go for thin sections but I need to stabilize the capsule, with ruthenium red per exemple, in order to avoid its collapse, but I am wondering if the affinity lectin/sugar residue will be affected by the stabilization.



Any ideas anyone?


Diane Montpetit
Centre de Recherche et de Développement sur les Aliments
3600 Boul. Casavant Ouest,
St-Hyacinthe, (Québec) Canada J2S 8E3
Téléphone/Phone: 450-773-1105
Télécopieur/ Fax: 450-773-8461
montpetitd-at-agr.gc.ca




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 13:31:29 2005



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Tue, 5 Apr 2005 15:43:58 -0400
Subject: [Microscopy] Scaning 3 1/4x 4 negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
We use Microtek ScanMaker 6800 with acceptable results.
Dorota


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 14:12:04 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 5 Apr 2005 15:28:05 -0400
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have the Epson Perfection 3200 scanner and like it very much.

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Tuesday, April 05, 2005 11:55 AM
To: microscopy-at-microscopy.com


I would like to find a suitable scanner to scan Kodak 4489 film, that is:
3.25" X 4" negatives. Preferably as low a priced system as possible would
be suitable, so I'm interested to see what others have done or are doing
when it comes to scanning this size of negative.

I've already noticed that when Nikon or Minolta talks about "electron
microscope film" they have in mind a format of 59mm X 82 mm, which is too
small for us.

Your help with valuable ideas would be GREATLY appreciated in this matter.



Garry Burgess

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg Canada

This e-mail and/or any documents in this transmission is intended for the
address(s) only and may contain legally privileged or confidential information.
Any unauthorized use, disclosure, distribution, copying or dissemination is
strictly prohibited. If you receive this transmission in error, please notify
the sender immediately and return the original.




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 14:12:21 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 05 Apr 2005 15:26:41 -0400
Subject: [Microscopy] Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,
I have an Epson Expression 1600 (its about 4 years old) that works
well for me. It has 1600 dpi resolution It does not have a holder
that fits the negatives, but it does have 2 focus settings (one for
film in holders and a 0mm distance for "contact prints"). As long as
my film is perfectly dry and I position it carefully, I have no
problems with Newton rings. I do scan it as positive film and then
reverse contrast in Photoshop, since the settings for scanning
negative film give really awful results.
When new, the scanner cost around $2,500, which at the time was half
of my budget for a new computer system. I don't what what the
current prices are, but I think that I get a lot of bang for my buck.
Just a happy user....
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 14:31:50 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Tue, 5 Apr 2005 15:44:20 -0400
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard,
You might try "Orthobeam" (though perhaps "Onthebeam" would
be more poetic?) or "Pairabeam".

TB


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 15:32:04 2005



From: Hicks, Aaron :      A.W.Hicks-at-massey.ac.nz
Date: Wed, 6 Apr 2005 08:47:35 +1200
Subject: [Microscopy] Re: Floor vibration measurements (was "EMU design

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Do you have a link for the magnetometer you use?

Aaron Hicks
Electron Microscopy Preparation Technician

Comparative Physiology and Anatomy
Institute of Veterinary, Animal, and Biomedical Sciences
Massey University

PN-412
Private Bag 11 222
Palmerston North
New Zealand

Phone +64 06 350 4874


-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Wednesday, 6 April 2005 4:32 a.m.
To: Hendrik O. Colijn
Cc: MSA listserver

I use a small magnetometer that plugs into my notebook PC. Using
Spectrum Laboratory Windows app. Free download is from here:

www . qsl. net/dl4yhf/spectra1.html

It was made by ham radio folks to recover Morse Code and
to do other radio things but is great for FFT analysis of
all sorts of vibration and acoustic noise.

gary g.
N6OIJ



At 07:43 AM 4/5/2005, you wrote:


} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} water. I reduced this effect by taking a large cardboard box (with
} appropriate cutouts) and placing it over the water dish.
}
} I used an old HeNe laser we had in the lab, but I suspect that with a
} little ingenuity, you could use a laser pointer just as well.
}
} Does anyone else have home-made environmental sensors for EM
} labs? (vibration, acoustics, EM fields, etc)?
}
} Cheers,
} Henk
}
} At 09:36 PM 04/04/05, Diana van Driel wrote:
}
}
} } ----------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} } was not exactly suited for an electron microscope. We had the choice
} } of the first or second floor. There was no choice of the underground
} } railway or the main road outside. As there was no money to pay for
} } proper testing, I spent a delightful day checking for vibration with a

} } saucer of water. I could be found for long periods at prospective
} } locations crouched on the floor with my saucer staring intently at the

} } water surface waiting for a train or the traffic lights to change. As
} } we in a hospital complex, this gave rise to several people
} } solicitously stopping and asking if I needed medical attention. I dare

} } say they were completely confused when I explained my mission; I
} } really should have thought up something more plausible, such as an
} } alien attack!
} }
} } As it turned out, the microscope is very happy and vibration free in
} } its second floor location!
} }
} } The moral? It may seem impossible, but if there's no choice and some
} } imagination.......
} }
} }
} } Diana van Driel
} } Dept Ophthalmology
} } Sydney University
} } GPO Box 4337
} } Sydney, NSW
} } AUSTRALIA 2001
} }
} } Phone 61 2 93827278
} } Mobile 0423 151614
} } FAX 61 2 93827318
} } On 1 Apr 2005, at 3:45 PM, Coetzee, Mr S. H Physics Science wrote:
} }
} } }
} } }
} } } ---------------------------------------------------------------------
} } } --
} } } -------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America

} } } shoved into a existing building. Some even of 2nd and 3rd floors.
} } } I will appreciate it if people can share there experience with
} } } relationship to performance problems observed, instrument involved
(W,
} } } LaB6 or FEG, SEM, ESEM, Duelbeem TEM etc.) How it was solved and a
} } } few nice and humorous examples will be great. I hope to get the
point
} } } across that an EM unit is a important part of a University, and if
} } } possible must be taken into consideration during building design and
} } } ultimately must play a part in the location decision of a University.
} } }
} } } Thanks
} } }
} } }
} } } Since some mail do get Lost, Bounces, etc Please send a
} } } duplicate/copy of all urgent mail to:
} } }
} } } coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}
} } }
} } } Mr S. H. Coetzee
} } } Electron Microscope Unit
} } } University of Botswana
} } } Private Bag 0022
} } } Gaborone
} } } Botswana
} } } Phone : +267 355 2462/5222
} } } Mobile : +267 718 36547
} } } Fax : +267 318 5097
} } } e-mail : coetzees-at-mopipi.ub.bw
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674 http://www.ceof.ohio-state.edu
} Time is that quality of nature which keeps events from happening all at
} once. Lately it doesn't seem to be working.





From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 15:48:37 2005



From: Ian Hallett :      IHallett-at-hortresearch.co.nz
Date: Wed, 06 Apr 2005 09:04:58 +1200
Subject: [Microscopy] Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Garry

I would second Ann Fook Yang's comments on the Epson 4870, it has a quoted DMax of 3.8 and certainly compares well with a dedicated negative scanner used by our photographic section. Like most scanners it does not have a negative holder that exactly fits the 4489 size but the films can be securely held by the 4" x 5" holder.

Ian



Ian Hallett
HortResearch
Mt Albert Research Centre, Private Bag 92 169
Auckland, New Zealand
Fax +64 9 815 4201
Telephone +64 9 815 4200
EMail ihallett-at-hortresearch.co.nz


} } } Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6/04/2005 3:54:32 a.m. } } }


------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I would like to find a suitable scanner to scan Kodak 4489 film, that is:
3.25" X 4" negatives. Preferably as low a priced system as possible would
be suitable, so I'm interested to see what others have done or are doing
when it comes to scanning this size of negative.

I've already noticed that when Nikon or Minolta talks about "electron
microscope film" they have in mind a format of 59mm X 82 mm, which is too
small for us.

Your help with valuable ideas would be GREATLY appreciated in this matter.



Garry Burgess

Charge Technologist - Electron Microscopy
Department of Pathology
Health Sciences Centre
Winnipeg Canada

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


______________________________________________________

The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 15:54:07 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Tue, 5 Apr 2005 15:08:24 -0600
Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Richard,

how about:

Double-beam
2-beam
Two-beam
FIB/SEM
SEM/FIB

Btw, I don't know if by making these suggestions here I would infringe or
perhaps gain copyright to these words. In any case, I don't mind if you use
any of them ;-)

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.Edu]
Sent: Tuesday, April 05, 2005 12:00
To: microscopy-at-MSA.Microscopy.com

Folks:

Help! I need a different name to call "Dualbeam" or "Crossbeam"
instruments since one is trademarked and the other is registered (FEI &
Zeiss).



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 16:00:18 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Tue, 5 Apr 2005 15:14:10 -0600
Subject: [Microscopy] Surface roughness using SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ed,

depending on the amplitude of the roughness, you could use Stereo imaging
and analysis. Essentially, you take a stereo pair and run it through
software to obtain a surface profile. We can do this with our stereo package
for the Scandium software, contact me off-line if you need further
information. The surface profile can then be analyzed to get roughness
parameters (r_sub_m, r_sub_q, etc.).

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: ekomarnicki-at-MacDermid.com [mailto:ekomarnicki-at-MacDermid.com]
Sent: Tuesday, April 05, 2005 12:05
To: microscopy-at-MSA.microscopy.com

Does any on the list know if one can perform surface roughness
measurements using a SEM either directly or through EDS software? I am
aware that this can be accomplished using AFMs and light and laser
profilometers, but is there software out there that allows the use of a
SEM?

TIA,

Ed Komarnicki
Sr. Analytical Chemist
MacDermid Inc.



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 16:58:29 2005



From: Tom Murray :      murraytm-at-u.washington.edu
Date: Tue, 5 Apr 2005 15:13:46 -0700
Subject: [Microscopy] Re: Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You could go with multi-beam. Even though it is only two it is
technically multi.


On Apr 5, 2005, at 12:44 PM, Tobias Baskin wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Richard,
} You might try "Orthobeam" (though perhaps "Onthebeam" would be more
} poetic?) or "Pairabeam".
}
} TB
}
}
} } ----------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ---------
} }
} } Folks:
} }
} } Help! I need a different name to call "Dualbeam" or "Crossbeam"
} } instruments since one is trademarked and the other is registered (FEI
} } &
} } Zeiss).
} }
} }
} }
} } Richard E. Edelmann, Ph.D.
} } Associate Editor of EXPO, Microscopy and Microanalysis Supplement
} } Electron Microscopy Facility Director
} } 350 Pearson Hall
} } Miami University, Oxford, OH 45056
} } Ph: 513.529.5712 Fax: 513.529.4243
} } E-mail: edelmare-at-muohio.edu
} } http://www.emf.muohio.edu
}
}
} --
} _ ____ __ ____ / \ / / \ /
} \ \ Tobias I. Baskin
} / / / / \ \ \ Biology Department
} /_ / __ /__ \ \ \__ 611 N. Pleasant St.
} / / / \ \ \ University of
} Massachusetts
} / / / \ \ \ Amherst, MA, 01003
} / / ___ / \ \__/ \ ____
} http://www.bio.umass.edu/biology/baskin/
} Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
}
}

------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email: murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 17:30:38 2005



From: hkonishi-at-indiana.edu
Date: Tue, 5 Apr 2005 17:46:18 -0500
Subject: [Microscopy] Si and O in lacey/holey C film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

I am wondering why Si and O are detected from commercial C films (two
companies). I measured C film and empty area (hole) next to the film, and I
only detected Si and O from C film. I think that Si and O really come from C
film.

I think that C was coated on plastic film with small holes. Commercial
lacey/holey C films are pure C or C on plastic. I don't think that plastic
contains Si.

I would appreciate any suggestion you might have.

Thank you,
Hiromi Konishi
IU and JHU


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 17:35:56 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 5 Apr 2005 16:07:02 -0700
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 5, 2005, at 10:59 AM, Richard Edelmann wrote:

} Help! I need a different name to call "Dualbeam" or "Crossbeam"
} instruments since one is trademarked and the other is registered (FEI &
} Zeiss).
}
Dear Richard,
How about "colliding beam"? I'm sure that the accelerator physics
people let that one get into the public domain.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 17:38:54 2005



From: George Theodossiou :      George.Theodossiou-at-amcor.com.au
Date: Wed, 6 Apr 2005 08:53:59 +1000
Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

BiBeam, DuoBeam, DeauxBeam, DiploBeam, MultiBeam......

-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.Edu]
Sent: Wednesday, 6 April 2005 04:00
To: microscopy-at-MSA.Microscopy.com

Folks:

Help! I need a different name to call "Dualbeam" or "Crossbeam"
instruments since one is trademarked and the other is registered (FEI &
Zeiss).



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu


************************************************************************
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information intended only for the use of the addressee named above.
If you are not the intended recipient of this message you are hereby
notified that any use, dissemination, distribution or reproduction of
this message is prohibited. If you have received this message in error
please notify AMCOR immediately.
Any views expressed in this message are those of the individual sender
and may not necessarily reflect the views of AMCOR.
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From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 18:28:44 2005



From: Ivan Petrov :      petrov-at-mrl.uiuc.edu
Date: Tue, 5 Apr 2005 18:44:45 -0500
Subject: [Microscopy] Job opening at CMM/UIUC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

FYI, this announcement is posted on the MSA web-site.

Regards

Ivan



University of Illinois at Urbana-Champaign

Frederick Seitz Materials Research Laboratory

SENIOR RESEARCH SCIENTIST IN ELECTRON MICROSCOPY

The Frederick Seitz Materials Research Laboratory at the University of
Illinois is seeking an experienced electron microscopist as a staff
member in the Center for Microanalysis of Materials. The Center is a
major DOE sponsored research facility for electron beam
microcharacterization and maintains state of the art electron microscopy
instrumentation as well as instruments for surface microanalysis, x-ray
diffraction and other analytical techniques.

The successful candidate will focus mainly on analytical STEM/TEM but is
expected to have the experience and the flexibility to work on other
techniques if needed. We are particularly interested in hiring a person
with experience in working with field emission TEMs, EDS and EELS
techniques. The Center houses five TEMs including a VG HB 501, JEOL
2010F, and is expected soon to acquire a Cs-corrected STEM instrument
with a monochromator and in-colunm energy filter. The person appointed
will have primary responsibility for these advanced instruments. The
successful candidate will also be responsible for facilitating the
research of approximately 50 users per year on advanced TEM and STEM
techniques. With the broad range of research programs active at the
Center, the position will offer ample opportunity to develop an
interactive research program as well as to pursue individual research
agenda.

This position requires a Ph.D. in Physics, Chemistry, Materials Science,
or related discipline with at least three years experience in advanced
electron microscopy techniques. Salary is commensurate with experience
and qualifications.

This is a full-time appointment with standard university benefits. The
proposed starting date is as soon as possible after the closing date.
In order to ensure full consideration, applications must be received by
May 15, 2005. Please send letter of application, resume, and names and
addresses of three references to Ivan Petrov, c/o Susan Logan,
University of Illinois, Materials Research Laboratory, 104 South Goodwin
Avenue, Urbana, Illinois 61801, phone (217) 244-2944.

The University of Illinois is an Affirmative Action/Equal Opportunity
Employer.


=====================================================================
Prof. Ivan Petrov, Director
Center for Microanalysis of Materials http://cmm.mrl.uiuc.edu
Frederick Seitz Materials Research Laboratory
University of Illinois tel: 217-333-8396
104 S. Goodwin Avenue fax: 217-244-2278
Urbana, IL 61801 USA email: petrov-at-uiuc.edu
=====================================================================



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 18:31:21 2005



From: Ronald Anderson :      randerson20-at-tampabay.rr.com
Date: Tue, 05 Apr 2005 19:46:28 -0400
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Substitute "column" for "beam" in any of the suggestions. Dual-Column
and Cross-Column seem to work.

Ron Anderson


Richard Edelmann wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 18:56:51 2005



From: :      lgiannuzzi-at-adelphia.net
Date: Tue, 5 Apr 2005 20:11:27 -0400
Subject: [Microscopy] Re: RE: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To avoid any such problems, we have referred to these instruments in the M&M "FIB" session as:

"Dual Platform" Instruments or how about

"Dual Column" Instruments or

"Multi-Column Platforms"

Lucille Giannuzzi
FEI Company


---- George Theodossiou {George.Theodossiou-at-amcor.com.au} wrote:
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} BiBeam, DuoBeam, DeauxBeam, DiploBeam, MultiBeam......
}
} -----Original Message-----
} } From: Richard Edelmann [mailto:edelmare-at-MUOhio.Edu]
} Sent: Wednesday, 6 April 2005 04:00
} To: microscopy-at-MSA.Microscopy.com
} Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name
}
}
}
} ----------------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America To



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 19:19:02 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 5 Apr 2005 17:50:08 -0700
Subject: [Microscopy] Re: Si and O in lacey/holey C film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 5, 2005, at 3:46 PM, hkonishi-at-indiana.edu wrote:

} I am wondering why Si and O are detected from commercial C films (two
} companies). I measured C film and empty area (hole) next to the film,
} and I
} only detected Si and O from C film. I think that Si and O really come
} from C
} film.
}
} I think that C was coated on plastic film with small holes. Commercial
} lacey/holey C films are pure C or C on plastic. I don't think that
} plastic
} contains Si.
}
} I would appreciate any suggestion you might have.

Dear Hiromi,
If the films are prepared by coating the plastic on a glass slide,
evaporating C onto the plastic, placing the coating onto grids (or
grids onto coating), then dissolving away the plastic, some of the Si
(and maybe O) could be removed from the glass and remain on the C even
after the plastic is dissolved, since the Si may be insoluble in the
organic solvent used to remove the plastic. If, however, the plastic
is cast onto water, picked up onto grids and C evaporated onto it
without using glass in any part of the process (except as a major
constituent of the bell jar in the evaporator), I would not expect to
see Si, although O is still possible. You could prepare films yourself
using each method and see if my expectations are borne out. I would be
interested in what the vendors who sell C-coated grids have to say.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 19:22:15 2005



From: somayyeh_kheiri-at-yahoo.com (by way of MicroscopyListserver)
Date: Tue, 5 Apr 2005 19:38:12 -0500
Subject: [Microscopy] viaWWW: thanks Dr Rosemary white

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 4, 2005 at 23:12:44
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh

Organization: urmia university

Title-Subject: [Microscopy] [Filtered] thanks Dr Rosemary white

Question: Dear Dr White

Thanks for replying me for the lenghth of FAA preserved material.
SO if the FAA shrinks the material what can I do with fruit
preserved in FAA?can I use these material or they have become unconsumable and useless?
and should I provide some other samples in a new FAA solution?


may this shrinking happen if I provide sectioning by microtomy or not?

thank you very much

Somayyeh

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 19:22:50 2005



From: guillet-at-chim.ucl.ac.be (by way of MicroscopyListserver)
Date: Tue, 5 Apr 2005 19:38:34 -0500
Subject: [Microscopy] viaWWW: osmium selective staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (guillet-at-chim.ucl.ac.be) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, April 5, 2005 at 12:33:44
---------------------------------------------------------------------------

Email: guillet-at-chim.ucl.ac.be
Name: Pierre

Title-Subject: [Microscopy] [Filtered] MListserver: osmium selective staining?

Question: Hi,
I'm looking for to choose a selective staining for polystyrene against polyethyleneoxyde. Before, I've tried ruthenium tetraoxide, but unsuccesfully.
I've plenty of hopes with osmium tetraoxide, but I don't know precisely its staining mecanism: do you think that polyethyleneoxide will be preserved from OsO4? Have you some tips about it?
Thank you.

Pierre GUILLET
PhD Student
University of Louvain-La-Neuve
Belgium

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 19:22:51 2005



From: montpetitd-at-agr.gc.ca (by way of MicroscopyListserver)
Date: Tue, 5 Apr 2005 19:38:54 -0500
Subject: [Microscopy] viaWWW: bacteria capsular material and lectins

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (montpetitd-at-agr.gc.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, April 5, 2005 at 12:34:18
---------------------------------------------------------------------------

Email: montpetitd-at-agr.gc.ca
Name: diane montpetit

Organization: agriculture canada

Title-Subject: [Microscopy] [Filtered] bacteria capsular material and lectins

Question: Hello all,

I need to stabilize the capsular material surrounding a bacteria, in order to see if the lectin I intend to use is directed againts a sugar residue standing on the capsular material or on the cell wall itself.

I have already looked at the bacteria with PTA (negative staining)and I am not satisfied. There is binding but it is still unclear, so I decided that I would go for thin sectioning.

But I believe I need to somehow stabilize the capsule, in order to avoid most of the collapsing, and I am wondering if doing so, using ruthenium red per exemple, I would affect the affinity lectin/sugar ?

Anyone has an idea?

thank you,
Diane

Diane Montpetit
Centre de Recherche et de DÈveloppement sur les Aliments
3600 Boul. Casavant Ouest,
St-Hyacinthe, (QuÈbec) Canada J2S 8E3
TÈlÈphone/Phone: 450-773-1105
TÈlÈcopieur/ Fax: 450-773-8461
montpetitd-at-agr.gc.ca




---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 22:31:23 2005



From: Luc Harmsen :      luc-at-anaspec.co.za
Date: Wed, 6 Apr 2005 05:46:11 +0200
Subject: [Microscopy] Re: [ Microscopy] EMU design lessons learned from the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As can be seen by the responses there are quite a variety of methods to
check a lab.
Unfortunately we are always required to do it the correct way and use the
expensive test kits.
In 90% of cases where we do a pre-install site check there is never a
problem for the applications of 80% of our customers.

I will define that. We install SEMs on Mine sites where at 12pm every day
they blast with dynamite. It's no problem. Its not that they get all the lab
staff to lift the SEM off the floor for that 1/2 a second. The SEM is
actually running at the time. They use the SEM to analyze rock particle
sections on a 24-7 basis. So, yes when the blast occurs possibly 1 or 2
particle sections may have a bit of a jaggered edge to them but in a total
of possibly 2000 sections in that run, who cares.

I would also dare to say for most SEM operators who never go over 10,000
times are even aware that their SEM may have vibration from old fans etc.

Vibration and fields only really start becoming a problem when you are
expecting to really drive the EM. FEG's and TEM's being used for
micrographing Diffraction patterns need to have a decent vibration and field
check done.

So my point here could really be that in a perfect world we would all pay
the service company to do a thorough job on a site inspection, but on realty
university budgets, in most cases the cup of water and working credit cards
after them being in a room can also work. It mostly depends on what you are
going to expect from the system.

Then the second consideration is; how easy can we move the unit, if that
method did not work. Most modern SEM's come on wheels. But try de-installing
a TEM if you find fields in the room you thought was OK. A bit more costly
than possibly having paid for the site check and sharing a coffee with you
friendly support engineer before the install. It's all about risk and who
gets blamed when it does not work.

Luc Harmsen
www.Anaspec.co.za


-----Original Message-----
} From: Diana van Driel [mailto:dianavd-at-eye.usyd.edu.au]
Sent: 05 April 2005 03:36
To: Coetzee, Mr S. H Physics Science
Cc: Microscopy

When we moved into our new building, a home had to be found for the
TEM. The new building, though beautiful and heritage listed sandstone,
was not exactly suited for an electron microscope. We had the choice of
the first or second floor. There was no choice of the underground
railway or the main road outside. As there was no money to pay for
proper testing, I spent a delightful day checking for vibration with a
saucer of water. I could be found for long periods at prospective
locations crouched on the floor with my saucer staring intently at the
water surface waiting for a train or the traffic lights to change. As
we in a hospital complex, this gave rise to several people solicitously
stopping and asking if I needed medical attention. I dare say they were
completely confused when I explained my mission; I really should have
thought up something more plausible, such as an alien attack!

As it turned out, the microscope is very happy and vibration free in
its second floor location!

The moral? It may seem impossible, but if there's no choice and some
imagination.......


Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
On 1 Apr 2005, at 3:45 PM, Coetzee, Mr S. H Physics Science wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Dear All
} I just love this list. We are having a workshop on Laboratory
} infrastructure and M {management.
} Most EM Units (like ours) I know was shoved into a existing building.
} Some even of 2nd and 3rd floors.
} I will appreciate it if people can share there experience with
} relationship to performance problems observed, instrument involved (W,
} LaB6 or FEG, SEM, ESEM, Duelbeem TEM etc.) How it was solved and a
} few nice and humorous examples will be great. I hope to get the point
} across that an EM unit is a important part of a University, and if
} possible must be taken into consideration during building design and
} ultimately must play a part in the location decision of a University.
}
} Thanks
}
}
} Since some mail do get Lost, Bounces, etc Please send a duplicate/copy
} of all urgent mail to:
}
} coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}
}
} Mr S. H. Coetzee
} Electron Microscope Unit
} University of Botswana
} Private Bag 0022
} Gaborone
} Botswana
} Phone : +267 355 2462/5222
} Mobile : +267 718 36547
} Fax : +267 318 5097
} e-mail : coetzees-at-mopipi.ub.bw
}





From MicroscopyL-request-at-ns.microscopy.com Tue Apr 5 23:58:37 2005



From: Ian Hallett :      IHallett-at-hortresearch.co.nz
Date: Wed, 06 Apr 2005 17:15:53 +1200
Subject: [Microscopy] Freeze Substitution Systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have any views on the relative merits of the RMC Boeckler FS-7500 and Leica EM AFS freeze substitution systems.

Thanks

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre, Private Bag 92 169
Auckland, New Zealand
Fax +64 9 815 4201
Telephone +64 9 815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________

The contents of this e-mail are privileged and/or confidential to the
named recipient and are not to be used by any other person and/or
organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
______________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 01:25:23 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 5 Apr 2005 23:40:23 -0700
Subject: [Microscopy] "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard;
I don't think you can trademark the English language. Dual and
Beam with a space between them can be used as a descriptor as long as
you use something else to be your tool's model name, and the tool itself
does not violate patents.

John Mardinly
408-765-2346
877-277-1182


-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.Edu]
Sent: Tuesday, April 05, 2005 11:00 AM
To: microscopy-at-MSA.Microscopy.com

Folks:

Help! I need a different name to call "Dualbeam" or "Crossbeam"

instruments since one is trademarked and the other is registered (FEI &
Zeiss).



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 03:54:23 2005



From: gillian.2.brown-at-gsk.com
Date: Wed, 6 Apr 2005 10:09:20 +0100
Subject: [Microscopy] Assessing and quantitation of mast cell degranulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I have posted a question about guinea-pig mast cells and their
demonstration (many thanks to Karen Bentley for the most successful
method). However we have moved on and we now need to actually catch
degranulation in progress. Due to the difficulty of guinea-pig we are
moving to the mouse. Other getting tissue quickly post the 'trigger' then
glut fixing, osmium and processing to resin and looking at the Tol blue
for purple/pink granules a la Dvorak, Blood 1994 83:3600-3612 and Oliani
2001 Cell Biol Int 25:795-803, does any-one know of an other method?
We are doing this in the nasal airways and will be taking lavages etc so
can measure tryptase histamine but these are considered circumstantial.
The tryptase antibody we use for human does not work in mouse but has
any-one any ideas for 'activated' mast cell markers tinctorial or IHC I
would be most grateful.

many thanks
Gillian Brown


Histopathology Group
GlaxoSmithKline Medicines Research Centre,





From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 07:13:47 2005



From: AtcSEM :      atcsem-at-sbcglobal.net
Date: Wed, 6 Apr 2005 08:29:24 -0400
Subject: [Microscopy] RE: Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How about "DuoBeam"?

Pavel Lozovyy
ATC SEM Lab
(216)692-6637
http://www.atclabs.com/SEM.htm
 
 
 
 


| -----Original Message-----
| From: Tobias Baskin [mailto:baskin-at-bio.umass.edu]
| Sent: Tuesday, April 05, 2005 3:44 PM
| To: edelmare-at-MUOhio.Edu
| Cc: microscopy-at-MSA.Microscopy.com
| Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name
|
|
|
| --------------------------------------------------------------------------
| ----
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe --
| http://www.microscopy.com/MicroscopyListserver
| On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
| --------------------------------------------------------------------------
| -----
|
| Richard,
| You might try "Orthobeam" (though perhaps "Onthebeam" would
| be more poetic?) or "Pairabeam".
|
| TB
|
|
| } -------------------------------------------------------------------------
| -----
| } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| } To Subscribe/Unsubscribe --
| http://www.microscopy.com/MicroscopyListserver
| } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
| } -------------------------------------------------------------------------
| ------
| }
| } Folks:
| }
| } Help! I need a different name to call "Dualbeam" or "Crossbeam"
| } instruments since one is trademarked and the other is registered (FEI &
| } Zeiss).
| }
| }
| }
| } Richard E. Edelmann, Ph.D.
| } Associate Editor of EXPO, Microscopy and Microanalysis Supplement
| } Electron Microscopy Facility Director
| } 350 Pearson Hall
| } Miami University, Oxford, OH 45056
| } Ph: 513.529.5712 Fax: 513.529.4243
| } E-mail: edelmare-at-muohio.edu
| } http://www.emf.muohio.edu
|
|
| --
| _ ____ __ ____
| / \ / / \ / \ \ Tobias I. Baskin
| / / / / \ \ \ Biology Department
| /_ / __ /__ \ \ \__ 611 N. Pleasant St.
| / / / \ \ \ University of
| Massachusetts
| / / / \ \ \ Amherst, MA, 01003
| / / ___ / \ \__/ \ ____
| http://www.bio.umass.edu/biology/baskin/
| Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 08:13:55 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 6 Apr 2005 08:28:51 -0500
Subject: [Microscopy] Re: Sputter Coating Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

After receiving all the very helpful replies on this topic, we decided
to severely limit the use of our machine for "industrial strength" uses.
Unfortunately I was about a week too late, and it looks like a board
fried, probably, I'm told, due to being run too hard for too long.
Except for an old conventionally pumped Au coater, we are down.....

So, a word to the wise out on the list----unless you have a } 15 year old
Emscope SC500, best keep your general use coaters for general use and
leave the heavy duty stuff to the big machines. And, David, can we
borrow your coater for a couple weeks?

Sputterlessly,
Randy

-----Original Message-----
} From: David Patton [mailto:David.Patton-at-uwe.ac.uk]
Sent: Wednesday, April 06, 2005 7:13 AM
To: Tindall, Randy D.

Hi All,

Many thanks for the replies to my original question on the scaling Pt
target and microcracks in the specimen coating. The consensus seems to
be that overheating is the problem.

I wasn't clear in my original posting about why we are doing this.
These specimens are not being coated for SEM viewing, but because we
have the only sputter coater on campus and the particular lab doing all
this work requires an extremely heavy layer of whatever metal they are
using---which may be Ni, Pt, or Ta. They are the ones driving the 90 mA
and repeated 4 minute cycles (because 4 minutes is the longest time we
can program into the coater and there is no manual on/off, if we want a
16 minute coat, we have to do 4 runs). This is very heavy use for a
coater designed for general EM use, rather than industrial strength use,
and I am concerned that it's beyond the design parameters of the
instrument and may leave us with a crippled coater (and a crippled lab).
We don't have the funds readily available to replace this machine if it
dies. Normally we run this instrument between 10 and 20 mA for 15
seconds to 3 minutes for SEM coating.

In terms of purging the chamber with argon, etc., this turbo pumped
EmiTech K575X runs on a programmed, automated cycle. The user's role is
to punch in the parameters and push the start button. It has been a
very reliable instrument after some initial setup problems were
corrected. We hope it remains so!

Thanks again, everybody.

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu









This incoming email to UWE has been independently scanned for viruses
and any virus detected has been removed using McAfee anti-virus software


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From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 08:46:58 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 06 Apr 2005 10:01:11 -0700
Subject: [Microscopy] Re: Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have an Epson Perfection 3200 and I just put the negative, emulsion
side up (yes up) on the bed and scan it as if it were a negative
transparency. Works fine. Dedicated film scanners that will do 3.25x4
film cost thousands of dollars.
Geoff

} } } } Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6/04/2005 3:54:32 a.m. } } }
} } } }
} } } }
} -----------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 08:57:51 2005



From: Stacey.Andringa-at-uc.edu (by way of MicroscopyListserver)
Date: Wed, 6 Apr 2005 09:13:52 -0500
Subject: [Microscopy] viaWWW: Fun stories about EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, April 6, 2005 at 06:26:44
---------------------------------------------------------------------------

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Microscopy] [Filtered] MListserver:Fun stories about EM

Question: Several of us were inspired by a previous listserv response
to collect interesting, funny, and odd stories about
our experiences in microscopy (especially those
historical and hysterical ones) to create a small compendium
to submit to one of the microscopy journals, or just to circulate
for nostalgic reading. Any and all stories are
welcome. If you contribute and want your name
withheld for some reason, please advise. If you have
imges (tifs, jpgs, gifs, or psds)they are welcome too.
Have fun, thanks, and have a great day, Marian L. Miller

Stacey Andringa
Marian Miller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 09:57:43 2005



From: frank.karl-at-degussa.com
Date: Wed, 6 Apr 2005 11:13:08 -0400
Subject: [Microscopy] I got 'em TEM blues....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,
I'm hoping I can get some advise on my Philips 430 TEM.

The problem appears that the filament, condenser aperture, specimen (lets
not forget those little guys)and objective aperture are not alined. I have
walked through the alinement procedure several times, but I continue to see
some odd artifacts:

At relatively low (6300X) mag I still see the edge of my objective aperture
in the plane of the specimen (I don't normally see this at this mag).

Going through cross-over I get a "dark field image" of my specimen
surrounding the bight field image on what appears to be an image of the
condenser aperture.

With the objective aperture out, I get a relatively even and circular
opening and closing cross over. When I put the objective aperture in the
cross over becomes egg shaped cross over. Centering either the objective
or condenser aperture doe's not seem to resolve the problem.

I have these problems with the specimen in or out.

Please feel free to contact me directly, if you think the response doesn't
merit the bandwidth.

Thanks........

Frank.karl-at-degussa.com


Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
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responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
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in or attached to this transmission is STRICTLY PROHIBITED. If you have
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by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 10:15:53 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 6 Apr 2005 10:31:19 -0500
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives - many thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would just like to thank everyone for their advice and comments on this
scanner question that I posted. I was able to research it fairly well in a
very short period of time.

I find this mailing list a huge and valuable resource to answer questions
like this, esp. when it comes to rapidly changing technology. I ended up
going with the Epson Perfection 4870 Photo because several people
recommended it, and the specs looked good, and the price was also very
reasonable, and I would be able to fit my negatives into the 4X5" holders
putting them crossways. I don't have it yet but people here have made me
feel confident about my decision. Thanks again.

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 11:10:59 2005



From: Ray D. Twesten :      twesten-at-uiuc.edu
Date: Wed, 6 Apr 2005 11:26:19 -0500
Subject: [Microscopy] RE: Si and O in lacey/holey C film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have on occasion seen batches of grids that are contaminated with
silicone. Most EM people will eschew silicone based vacuum greases and
fluids, but I do not know if all the makers of carbon films are as careful.
The times that I have seen this, the manufacture was happy to replace the
grids.

I have also seen hydrocarbon solvents that are contaminated with silicon.
The result is the grid is clean until a user places their suspended sample
on the grid. Using a bare grid will confirm this.

If you have silicone contamination and you focus a small spot on the sample,
you will see a rapidly growing contamination spot. EDS will verify this
spot is Si and O rather than carbon.

Hope this helps, Ray

Ray D. Twesten, PhD.
Center for Microanalysis of Materials
Seitz Materials Research Laboratory
104 S. Goodwin Ave., Urbana, IL 61801
+1 217 244-6177 (Fax -2278)


} -----Original Message-----
} From: hkonishi-at-indiana.edu [mailto:hkonishi-at-indiana.edu]
} Sent: Tuesday, April 05, 2005 5:46 PM
} To: microscopy-at-microscopy.com
} Subject: [Microscopy] Si and O in lacey/holey C film
}
}
}
}
} ------------------------------------------------------------------
} ------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------
} -------------
}
} Hello,
}
} I am wondering why Si and O are detected from commercial C films (two
} companies). I measured C film and empty area (hole) next to the
} film, and I
} only detected Si and O from C film. I think that Si and O really
} come from C
} film.
}
} I think that C was coated on plastic film with small holes. Commercial
} lacey/holey C films are pure C or C on plastic. I don't think
} that plastic
} contains Si.
}
} I would appreciate any suggestion you might have.
}
} Thank you,
} Hiromi Konishi
} IU and JHU
}



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 11:23:37 2005



From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Wed, 6 Apr 2005 12:39:16 -0400
Subject: [Microscopy] Jet Polishers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are in the market for a twin (preferably) jet polisher for TEM foils.
I searched the List Server archives and found that this question was
discussed in 1997.
Is there any improvement since then?
The players then were:
Fischione
South Bay
Struers.
Anyone have any experience with any of them?
The South Bay unit looks suspiciously like the Struer's unit anyone
know who actually makes it?
Anyone have recommendations or horror stories?

Email please and I'll summarize if there are other parties interested.

John Mansfield PhD CPhys CSci MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143 USA
Phone: (734) 936-3352
FAX (734) 763-2282
Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"
AIM: thejfmjfm
Yahoo: thejfmjfm
Skype: thejfmjfm, (preferred)





From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 11:34:33 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 6 Apr 2005 10:09:14 -0700
Subject: [Microscopy] Re: I got 'em TEM blues....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Garry

I would seriously looked at the Microtek type of flatbed scanner. It is
similar to the old AGFA Duoscan having a standard A4+ glass flatbed and
a similar size glassless film drawer with glassless holders for
negatives and transparencies up to A4. Check out the i900 below:
http://www.microtekusa.com/smi900.html

or other dual scan glassless flatbeds:
http://www.microtekusa.com/pp.html

I have been using the Microtek Scanmaker 8700 for some time and have
been very impressed, yet it's specification is blown away by the new
Microtek. It appears to be offering 3200x6400dpi resolution, 4.2 Dmax
(ie dense negatives and 48 bit colour - 16 bit B&W). I still use the
original 4x5 inch glassless holders on my 3.25 x 4inch negatives but I
am planning to get a special holder made up.

The only annoying quibble that I have is the i900 system comes with
digital 'ICE' for reduction of dust and scratches but this is only
available for prints - not negatives. So it may be worth checking if
there is a version that has 'ICE' for negatives/transparencies (in
fairness I think that only the Epson 4700 has 'ICE' on a normal
flatbed).

The i900 is about 600 US dollars (I,ve seen an advert for 60 dollars
less as well) but that's a lot cheaper than a true dedicated large film
scanner. I don't know how the prices compare in the USA with the Epson
4700 but the Epson is copying negatives on a glass bed which may mark.
gather dust or create Newtons's Rings.

I have no connection with Microtek other than as a satisfied user and I
was very happy with my old Epson 1200 scanner before that.


Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk




----- Original Message -----
} From: Geoff McAuliffe {mcauliff-at-umdnj.edu}


On Apr 6, 2005, at 8:13 AM, frank.karl-at-degussa.com wrote:

} I'm hoping I can get some advise on my Philips 430 TEM.
}
} The problem appears that the filament, condenser aperture, specimen
} (lets
} not forget those little guys)and objective aperture are not alined. I
} have
} walked through the alinement procedure several times, but I continue
} to see
} some odd artifacts:
}
} At relatively low (6300X) mag I still see the edge of my objective
} aperture
} in the plane of the specimen (I don't normally see this at this mag).
}
} Going through cross-over I get a "dark field image" of my specimen
} surrounding the bight field image on what appears to be an image of the
} condenser aperture.
}
} With the objective aperture out, I get a relatively even and circular
} opening and closing cross over. When I put the objective aperture in
} the
} cross over becomes egg shaped cross over. Centering either the
} objective
} or condenser aperture doe's not seem to resolve the problem.
}
} I have these problems with the specimen in or out.
}
} Please feel free to contact me directly, if you think the response
} doesn't
} merit the bandwidth.
}
Dear Frank,
From your description it looks like the filament, condenser aperture
and specimen are OK. I surmise this from the fact that with the
objective aperture out the beam spot expands evenly on both sides of
crossover, and that you do not report image motion when the condenser
is changed. I also assume that if the magnification were much
different from the indicated value, you'd have noticed and reported it,
and that you are not in some weird defocus condition (although this
would account for the dark-field-surrounding-bright-field effect, which
sounds somewhat like defocussed diffraction). Having the objective
aperture visible in the specimen plane sounds like a low mag condition,
where the objective aperture becomes an area-selecting aperture, and I
wonder if the "egg shaped cross over" is the edge of the objective
aperture occluding the beam. Have you checked all the lens currents?
If some of them are not at the usual values for the microscope
settings, there may be problems with the electronics. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 11:44:40 2005



From: MIKO(Lianfeng) :      lffu-at-ucdavis.edu
Date: Wed, 6 Apr 2005 10:00:26 -0700 (PDT)
Subject: [Microscopy] POSTDOCTORAL POSITIONS AVAILABLE IN UC-DAVIS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


POSTDOCTORAL POSITIONS IN INTERFACE PHYSICS

DEPARTMENT OF CHEMICAL ENGINEERING AND MATERIALS SCIENCE
UNIVERSITY OF CALIFORNIA-DAVIS

3 postdoctoral positions are available in the Interface Physics Group at
the University of California-Davis (UCD). Research in the Interface
Physics Group focuses on the use of atomic resolution imaging/analytical
techniques and newly developed dynamic measurement capabilities (sub-ns
time resolution) in electron microscopy, coupled with theoretical
simulations, to determine the structure-property relationships in nanoscale
systems. The positions that are currently available are related to high-Tc
superconductors, semiconductor quantum dots and the development of
nanoscale programs for the dynamic TEM. Successful candidates will be
recent Ph.D. graduates in physics, metallurgy, or materials science with a
sound background in the relevant materials issues and an ambition to be
part of a developing program pushing at the frontiers of interface
physics. Please send a resume and publication list to Professor Nigel D.
Browning at the address below. Prior experience in STEM or TEM is
essential. However, consideration will be based on the candidates overall
potential for success in the field and applicants with prior experience in
related fields are encouraged to apply. Positions are for one year
initially, normally renewed for a second year with possibilities existing
for further years. Salary is commensurate with experience.


Nigel D. Browning

Department of Chemical Engineering and Materials Science
University of California-Davis
One Shields Ave
Davis, CA 95616

AND

National Center for Electron Microscopy, MS 72-150
Lawrence Berkeley National Laboratory
Berkeley, CA 94720

Tel: 530-754-5358 (Davis)
510-486-4612 (NCEM)
Fax: 530-752-9554 (Davis)
510-486-5888 (NCEM)
e-mail: nbrowning-at-ucdavis.edu
ndbrowning-at-lbl.gov







From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 12:29:21 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 6 Apr 2005 13:45:59 -0400
Subject: [Microscopy] Re: Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Actually, that's what we do too with our Epson 3200--works nicely.

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: Geoff McAuliffe [mailto:mcauliff-at-umdnj.edu]
Sent: Wednesday, April 06, 2005 1:01 PM
To: microscopy-at-microscopy.com

I have an Epson Perfection 3200 and I just put the negative, emulsion
side up (yes up) on the bed and scan it as if it were a negative
transparency. Works fine. Dedicated film scanners that will do 3.25x4
film cost thousands of dollars.
Geoff

} } } } Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6/04/2005 3:54:32 a.m. } } }
} } } }
} } } }
} -----------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America







From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 12:54:15 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 06 Apr 2005 14:10:05 -0500
Subject: [Microscopy] Automatic vs manual desiccator storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Michael Zemyan wrote:
=========================================================
In reading the description of these automatic dessicators, I notice that
they have 2 fans, one of which continually circulates air inside the chamber
Does anyone know, are the fan bearings oil-lubricated? Is this
potentially a source of oil vapor contamination? We use dessicators (not
the automatic kind) to store cleaned vacuum parts, and so oil is a concern.
===========================================================
With regard to the Secador Automatic desiccator cabinets, see URL
http://www.2spi.com/catalog/supp/secador-desiccator-cabinets-4-0-vertical.
shtml

A ball bearing fan is used and these ball bearings are never oiled, however
they do contain oil. We have never heard of any reports however of anyone
reporting oil contamination of otherwise clean parts after sitting in the
environment of a Secador cabinet. I guess the acid test would be to do XPS
on similar parts stored, manual desiccator vs. automatic.

If you do this experiment, please share your results with us!

Disclaimer: SPI Supplies offers the Secador automatic desiccating cabinets,
both automatic as well as "manual" so if you don't like the automatic
feature, we would be happy to have you consider the manual version!

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 13:14:53 2005



From: Gordon Vrololjak :      gvrdolja-at-nature.berkeley.edu
Date: Wed, 6 Apr 2005 11:30:31 -0700 (PDT)
Subject: [Microscopy] sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Since the topic of sputter coating has come up, I had a question I was
wondering about. We have a Balzers/technotrade MED020 sputter coater.
When coating, sometimes I have trouble generating the plasma. It will go
through arcing. If I adjust the argon flow to give a good amount of argon
in the chamber I can usually get the plasma to ignite better and have more
stability, then I can turn the argon flow down. Sometimes though, the
plasma will not ignite at all. I'm stumped by the problem and was
wondering if anyone had some advice?

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 13:33:42 2005



From: Yang, Ann-Fook :      YANGA-at-agr.gc.ca
Date: Wed, 6 Apr 2005 14:46:38 -0400
Subject: [Microscopy] Scanning 3 1/4" X 4" Negatives - many thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I find that the 4" X 5" film holder is clumsy to use. I had asked my machinist to cut two 31/4" X 4" holes, each with a 1 mm thick lip and a cut- out for lifting the negative, out of a black plastic sheet. You may have four holes cut from a plastic sheet; I chose two because I was afraid that four holes would weaken the plastic too much.
Placing the negatives on the glass is workable, but, one may get Newton rings.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
} From: Garry Burgess [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Wednesday, April 06, 2005 11:31 AM
To: microscopy-at-microscopy.com


I would just like to thank everyone for their advice and comments on this
scanner question that I posted. I was able to research it fairly well in a
very short period of time.

I find this mailing list a huge and valuable resource to answer questions
like this, esp. when it comes to rapidly changing technology. I ended up
going with the Epson Perfection 4870 Photo because several people
recommended it, and the specs looked good, and the price was also very
reasonable, and I would be able to fit my negatives into the 4X5" holders
putting them crossways. I don't have it yet but people here have made me
feel confident about my decision. Thanks again.

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From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 13:42:51 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 6 Apr 2005 13:58:27 -0500
Subject: [Microscopy] New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in purchasing a new Ultramicrotome for sectioning in
diagnostic pathology to replace an aging Reichert that has been giving us
some problems. I have used Reichert Ultramictrotomes for the last 21 years,
and now they have become Leica, but I was wondering what opinions that
people have other other ultramicrotomes from other manufacturers, especially
new ultramicrotomes, such as the Powertome X or XO from RMC Products. Are
there other ultramicrotomes on the market that I should know about?

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 14:24:59 2005



From: Lois Anderson :      landers-at-jhmi.edu
Date: Wed, 06 Apr 2005 15:39:33 -0400
Subject: [Microscopy] Re: Automatic vs manual desiccator storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are very much interested in an automated film desiccator. Does
anyone have one or know who manufactures one?

Lois Anderson
Johns Hopkins University
Dept. of Pathology
Laboratory Manager
EM/IF/Reference Histology
600 N. Wolfe Street/Pathology 709 A
Baltimore, MD 21287
(410) 955-2861/fax (410) 614-7110
landers-at-jhmi.edu



} } } "Garber, Charles A." {cgarber-at-2spi.com} 04/06/05 3:10 PM } } }


------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Michael Zemyan wrote:
=========================================================
In reading the description of these automatic dessicators, I notice
that
they have 2 fans, one of which continually circulates air inside the
chamber
Does anyone know, are the fan bearings oil-lubricated? Is this
potentially a source of oil vapor contamination? We use dessicators
(not
the automatic kind) to store cleaned vacuum parts, and so oil is a
concern.
===========================================================
With regard to the Secador Automatic desiccator cabinets, see URL
http://www.2spi.com/catalog/supp/secador-desiccator-cabinets-4-0-vertical.
shtml

A ball bearing fan is used and these ball bearings are never oiled,
however
they do contain oil. We have never heard of any reports however of
anyone
reporting oil contamination of otherwise clean parts after sitting in
the
environment of a Secador cabinet. I guess the acid test would be to do
XPS
on similar parts stored, manual desiccator vs. automatic.

If you do this experiment, please share your results with us!

Disclaimer: SPI Supplies offers the Secador automatic desiccating
cabinets,
both automatic as well as "manual" so if you don't like the automatic
feature, we would be happy to have you consider the manual version!

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com

West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


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From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 14:39:47 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 06 Apr 2005 15:35:04 -0500
Subject: [Microscopy] Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gordon:

If I remember correctly, you can look to confirm this yourself, that the
argon inlet is at the bottom of the chamber NOT anywhere near the
sputterhead, imagine that! I found that most of the argon was going right
down the throat of the TMP.. The fix is to get a small SST tube that will
fit snuggly into the Argon inlet aperture and bend it around so it goes
around all of the other hardware in the chamber and end up about 1 cm lower
than the sputter head when sealed to the bell jar. This should do the trick
by actually putting the gas where it's needed, an advanced concept my
friends from "Happy Valley" didn't think of when designing the beast.

Should you care to discuss it further feel free to give me a call.

Cheers!


Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com


----- Original Message -----
} From: "Gordon Vrololjak" {gvrdolja-at-nature.berkeley.edu}
To: {microscopy-at-msa.microscopy.com}
Sent: Wednesday, April 06, 2005 2:30 PM

HI, Garry,

Leica, which is the company which acquired the Reichert microtome business
as part of the Cambridge-Leitz merger in 1990, came out last year with the
UC6. I had a chance to see it "up close and personal" at Cell Bio last
December and to work with it a little as part of a new AFM/ultramicrotome
integration for both bio and polymer applications. The same people who
sold your Reichert (ex: Ann Korsen) are with Leica and can answer your
questions. Ann can be reached at Akorsen-at-leica-microsystems.com

Hope this is helpful.

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to
learn more about "Optimizing LIght Microscopy". Copies still available
through MME... even for class-room lots ... and we give quantity discounts.



At 01:58 PM 4/6/2005, Garry Burgess wrote:


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From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 15:35:56 2005



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Wed, 6 Apr 2005 21:48:04 +0100
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Although I do have a vested interest, quite honestly I can't see that
'legal' protection for these terms achieves anything for anybody
apart from feeding the lawyers. In both cases, they are hardly more
than a simple description.

Personally, I'd suggest everybody use both as much as possible to
emphasise what nonsense it is.
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 16:35:08 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 06 Apr 2005 18:41:32 -0500
Subject: [Microscopy] Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

MKS http://www.mksinst.com/ was making a product in the past that might work
nicely. It is a tiny UV gas discharge lamp mounted in a standard vacuum
fitting. 120V AC, plugs into wall outlet. It is intended for starting up and
maintaining stable operation of cold cathode (Penning) vacuum sensors at
very low pressures (down to 10 -10 Torr). Description was "Cold Cathode
Starting Device", IgniTorr, part #100006850 (for 120V AC), used to cost
$220.

Even if it is discontinued, similar devices must be available from
semiconductor process tools suppliers (plasma etch, vac. vapor deposition,
etc.). In fact, any similar low power slow discharge UV lamp will work, just
find a way of placing it anywhere inside the process chamber. The one used
in IgniTorr has 2mA current and 55V breakdown voltage. It was connected to
120V AC line through 60K Ohm resistor. An isolation transformer wouldn't
hurt, plus, you will need only single pole vacuum connector.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane
Duluth, GA 30096
Tel. (770)232-7785
Fax (770)232-1791
Mobile (678)467-0012
www.sia-cam.com
----- Original Message -----
} From: "Gordon Vrololjak" {gvrdolja-at-nature.berkeley.edu}
To: {microscopy-at-msa.microscopy.com}
Sent: Wednesday, April 06, 2005 2:30 PM

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Larry Stoter wrote:
=================================================================
Although I do have a vested interest, quite honestly I can't see that
'legal' protection for these terms achieves anything for anybody apart from
feeding the lawyers. In both cases, they are hardly more than a simple
description.

Personally, I'd suggest everybody use both as much as possible to emphasise
what nonsense it is.
==================================================================
A trade name is someone's intellectual property right. It is analogous to
ownership in a patent.

It is also the only way a customer has of differentiating between products
of one brand vs. another (which indeed might have entirely different
properties and qualities). In microscopy, keeping track of brand identity
could be the difference in being able to reproduce someone else's results.
Indeed the major use of trade names on this listserver would show that as an
industry we do understand the importance of recognizing brand differences.

I would respectfully maintain that respecting someone's trade name is not
"nonsense". And to not respect such intellectual property rights and use
trade names incorrectly, is also illegal in most countries.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 20:07:51 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 6 Apr 2005 18:22:48 -0700
Subject: [Microscopy] Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chuck;
Maybe a parallel could be seen in the auto industry where they
have a larger legal budget. Did auto company ever register 'Dual
Exhausts'? Dual is just a simple adjective, and to claim exclusive right
to that would be to hijack the English language. 'Magic Tip', on the
other hand, is a delightfully creative assembly of words, and
registering that does not hijack the English language. Anyway, who knows
how it could be decided when too many lawyers get involved. Witness the
spat in the courts now over Smucker's attempt to patent the peanut
butter and jelly sandwich!

http://www.msnbc.msn.com/id/7408857/

John Mardinly

-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: Wednesday, April 06, 2005 4:42 PM
To: MICROSCOPY BB

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Larry Stoter wrote:
=================================================================
Although I do have a vested interest, quite honestly I can't see that
'legal' protection for these terms achieves anything for anybody apart
from
feeding the lawyers. In both cases, they are hardly more than a simple
description.

Personally, I'd suggest everybody use both as much as possible to
emphasise
what nonsense it is.
==================================================================
A trade name is someone's intellectual property right. It is analogous
to
ownership in a patent.

It is also the only way a customer has of differentiating between
products
of one brand vs. another (which indeed might have entirely different
properties and qualities). In microscopy, keeping track of brand
identity
could be the difference in being able to reproduce someone else's
results.
Indeed the major use of trade names on this listserver would show that
as an
industry we do understand the importance of recognizing brand
differences.

I would respectfully maintain that respecting someone's trade name is
not
"nonsense". And to not respect such intellectual property rights and
use
trade names incorrectly, is also illegal in most countries.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================






From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 20:11:57 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 06 Apr 2005 18:29:06 -0700
Subject: [Microscopy] Re: Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I had terrible experience with Ann Korsen - she basically did not returned
telephone calls and ignored my E.mails. At one single instance when I had
a chance to talk to her, she was quite helpless and rigid. I am sorry,
this is my personal experience on the way to purpose new ultratome. I also
had multiple problems a couple years ago when my UCT needed
service. Finally, some problems were resolved (not in my favor to be
truthful, on some I just gave up) but I still have a feeling that Leica's
personnel was sort of unfriendly to me. To me, Leica provides miserable 5%
"educational" discount (as a huge favor to me), they do not exchange/return
stuff at all: illuminator I ordered for UC6 appeared to be for UCT and they
denied to exchange (unopened box) or get it back. Spare-parts box for UCT
was broken, it took more than year for Leica to replace it. Knifemaker was
at least a month late than promised... In my last purpose, they were trying
to "sweet" my experience with Leica including some free stuff. Paul
DeGeorge was quite nice after all, I appreciate it. Nevertheless, having
terrible customer service (from my experience), they have superior
instruments. I also have to admit that I never ever had any negative
experience (only positive) with people from RMC - they are extremely
helpful and flexible. I don't have any interest in Leica or RMC: I am
using the products from both companies. Sergey

At 01:35 PM 4/6/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 21:31:23 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 06 Apr 2005 21:47:16 -0500
Subject: [Microscopy] Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have a student who is trying to grow cultured cells on coverslips to use
them for SEM imaging. However, the cells either do not stay on the
coverslips when they are transported to our lab or tend to lift during
preparation for SEM. Growth conditions, etc are below.

Cell media: Dulbecco's Mod. Eagle Medium, 4.5g/L glucose, Na pyruvate, L-
glutamine. The cells are grown in the presence of 10% FBS, but the FBS is
removed from the media the day before the experiment.

Coverslips: Thermanox plastic coverslips, cell culture treated on one side,
purchased from VWR. Glass coverslips have also been tried and actually
worked better than the thermanox.

Cells: Caco-2 cells, grown for 8-10 days prior to conducting experiment.

Fixation is with 3% glutaraldehyde in phosphate buffer followed by 1%
osmium, ETOH series and critical pint dry. Samples are handled very gently
when solutions are changed.

We usually have little trouble with cultured cells but this one has been
nothing but problems. Suggestions for growth or SEM prep would be
appreciated.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 6 21:44:43 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 06 Apr 2005 20:00:34 -0700
Subject: [Microscopy] Re: RE: Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The issue is the trademark as it is registered.

The holder has a responsibility to defend the trademark.
If not, the mark is lost. If I call my company Intel or
Microsoft or AMD or Micron, or whatever, is that OK?

No. These are registered trademarks and are not useable
without attribution. And they cannot be usurped or owned.

The trademark has other more powerful yet obscure values.
A registered trademark that is a TLD overpowers any one
else trying to use it as a domain name. This is of course
based on the TM or TLD being registered before an attempted
infringement.

Trademarks are valuable and costly. They are to be protected.
I have one and I protect it.

gary g.


At 06:22 PM 4/6/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 00:27:19 2005



From: Coetzee, Mr S. H Physics Science :      COETZEES-at-mopipi.ub.bw
Date: Thu, 7 Apr 2005 07:47:07 +0200
Subject: [Microscopy] RE: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Debby
Quite some time ago I was involved sorting the a similar problem for a Masters degree Student, Denise Lindsey in microbiology at WITS University, Johannesburg, South Africa. She work on bio films. Might work equally well for Caco-2 cells. I am not sure if she ever published the sample preparation technique. We resorted to polished Stainless steel squares with a small hole in one corner. The surface finish was polished to 1200# SiC grinding paper. If the surface is to rough you do not get a good view the cells and if it is to smooth you loose to many cells during processing. To that we tied a thin string through the whole to handle it through the fixation and Dehydration process. Worked beautifully. They are reusable and have some direct industrial application in the case of biofilms. Give less charging problems in conventional SEM. Apparently she finished a PhD and is still in academia. The best person to contact is her former supervisor Prof. Alex von Holy. He should be able to get you in contact with her.
His e-mail is: alex-at-gecko.biol.wits.ac.za
I am also copying him in the mail.
Hope this helps

} -----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
} Sent: Thursday, April 07, 2005 4:47 AM
} To: message to: MSA list
} Subject: [Microscopy] Cultured cell prep for SEM
}
}
}
}
} --------------------------------------------------------------
} ----------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} -----------------
}
} Hi all,
}
} I have a student who is trying to grow cultured cells on
} coverslips to use
} them for SEM imaging. However, the cells either do not stay on the
} coverslips when they are transported to our lab or tend to lift during
} preparation for SEM. Growth conditions, etc are below.
}
} Cell media: Dulbecco's Mod. Eagle Medium, 4.5g/L glucose, Na
} pyruvate, L-
} glutamine. The cells are grown in the presence of 10% FBS,
} but the FBS is
} removed from the media the day before the experiment.
}
} Coverslips: Thermanox plastic coverslips, cell culture
} treated on one side,
} purchased from VWR. Glass coverslips have also been tried and actually
} worked better than the thermanox.
}
} Cells: Caco-2 cells, grown for 8-10 days prior to conducting
} experiment.
}
} Fixation is with 3% glutaraldehyde in phosphate buffer followed by 1%
} osmium, ETOH series and critical pint dry. Samples are
} handled very gently
} when solutions are changed.
}
} We usually have little trouble with cultured cells but this
} one has been
} nothing but problems. Suggestions for growth or SEM prep would be
} appreciated.
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 02:27:46 2005



From: Victoria Fink :      vfink-at-shaw.ca
Date: Thu, 07 Apr 2005 00:43:12 -0700
Subject: [Microscopy] Re: RE: Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Gary,

Completely agree. I believe when somebody selecting trademark, or name of
the company a few optional names should be provided by the applying person.
You should pay for the name search prior a registration. And they usually
checking up our options. If none of them will not go through you will need
to do this again. I would suggest to check out already known names on
similar products to avoid to pay again. You should be creative enough to
select this name. If you have created product, I sure you will be able to
create a name for it. This is not necessary should be name of your product,
it could be name of your lovely person ( as "Mercedes" did) , or anything
you like, or anything you have invented in this model, any language: classic
ones, bible, anything. When you designed your product you did it with
passion as usually creative people do. Try to concentrate on the one or two
worlds you would express your passion, or what does it mean for you, or your
customers benefit/s. Good luck!

Victoria

----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com}
Sent: Wednesday, April 06, 2005 8:00 PM



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 04:36:24 2005



From: yezer-at-cc.hut.fi
Date: Thu, 07 Apr 2005 12:50:55 +0300 (EEST)
Subject: [Microscopy] HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I am using Hitachi S-4700 FESEM (cold tip) and so far didn’t succeed to achieve
a SE image with resolution better than 10 nm (including in the ultra high-
resolution mode). Can anyone help us with a guide lines how to reach high
resolution by controlling the parameters (Ie, Cond lens, and etc) with this
microscope?.

Thanks,

E. Yossef
Helsinki University of Technology
Physical Metallurgy and Materials Science


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 05:40:46 2005



From: George Theodossiou :      George.Theodossiou-at-amcor.com.au
Date: Thu, 7 Apr 2005 17:47:38 +1000
Subject: [Microscopy] Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Or the bitter fight over ownership of the term 'Ugg Boots'. That
delightfully australian sheepskin footwear that is oh so comfortable and oh
so warm.



-----Original Message-----
} From: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Thursday, 7 April 2005 11:23
To: Garber, Charles A.; MICROSCOPY BB

Chuck;
Maybe a parallel could be seen in the auto industry where they have
a larger legal budget. Did auto company ever register 'Dual Exhausts'? Dual
is just a simple adjective, and to claim exclusive right to that would be to
hijack the English language. 'Magic Tip', on the other hand, is a
delightfully creative assembly of words, and registering that does not
hijack the English language. Anyway, who knows how it could be decided when
too many lawyers get involved. Witness the spat in the courts now over
Smucker's attempt to patent the peanut butter and jelly sandwich!

http://www.msnbc.msn.com/id/7408857/

John Mardinly

-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: Wednesday, April 06, 2005 4:42 PM
To: MICROSCOPY BB

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Larry Stoter wrote:
=================================================================
Although I do have a vested interest, quite honestly I can't see that
'legal' protection for these terms achieves anything for anybody apart from
feeding the lawyers. In both cases, they are hardly more than a simple
description.

Personally, I'd suggest everybody use both as much as possible to emphasise
what nonsense it is.
==================================================================
A trade name is someone's intellectual property right. It is analogous to
ownership in a patent.

It is also the only way a customer has of differentiating between products
of one brand vs. another (which indeed might have entirely different
properties and qualities). In microscopy, keeping track of brand identity
could be the difference in being able to reproduce someone else's results.
Indeed the major use of trade names on this listserver would show that as an
industry we do understand the importance of recognizing brand differences.

I would respectfully maintain that respecting someone's trade name is not
"nonsense". And to not respect such intellectual property rights and
use
trade names incorrectly, is also illegal in most countries.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================






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From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 06:19:58 2005



From: ech :      ech-at-interchange.ubc.ca
Date: Thu, 07 Apr 2005 04:35:56 -0700 (PDT)
Subject: [Microscopy] Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Garry
we have three OM3s, an Ultracut E and an Ultracut T, all Leica. For the International Cryo EM Course last year, Leica brought in the UC6 which we got to thoroughly test. We really liked it.

They have made so many improvements on it that we (by that I actually mean George Postuma, Philip Hyam and Paul de George) easily had the course participants cutting cryo sections in a very short time. Three UC6's were bought by new faculty at UBC for their own labs in the next year and I believe them to be satisfied customers. These don't have cryo capability; they use our cryo system if they need it. Since we have George at the cryo course, we have been using the Tokuyasu method in our lab a lot more. He makes it very easy. The advantages of course being that the immunogold penetrates the whole section as there is no resin to get in the way. We used to have a lot of trouble with this method until George came from Jan Slot's lab gave us his experience.

We have cryo cutting ability on the Ultracut E, but the ease of use of the Ultracut E and UC6 is night and day. Leica are bringing UC6s to the cryo course this year. One thing we hope to try is high pressure freezing, cryo planing (UC6) and transfering onto the cryo stage of the cryo sem. We will also be trying the new Bal-Tec cryo transfer device to go from the high pressure freezer to the cryo-sem. I haven't advertised the cryo course on the website this year as there are only two places left. This year Helmut Gnaegi from Diatome will also be coming to teach cutting techniques.

I am sorry that I have no experience of the other products that you mentioned. Will you be going to the Microscopy Society of Canada meeting in Hamilton in May? Maybe we can touch base then as I would love to network and find out about these other products.

As you are in Canada, I guess Philip Hyam is your Leica rep too. We have always had very good dealings with him. We have someone in Vancouver who is very good at servicing our microtomes which is an added benefit and probably the most important thing about buying a new piece of equipment. At least for me running a facility.



----Original Message-----

} Date: Wed Apr 06 11:58:27 PDT 2005
} From: "Garry Burgess" {GBurgess-at-exchange.hsc.mb.ca}
} Subject: [Microscopy] New Ultramicrotomes
} To: "Microscopy MSA" {microscopy-at-microscopy.com}
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} I am interested in purchasing a new Ultramicrotome for sectioning in
} diagnostic pathology to replace an aging Reichert that has been giving us
} some problems. I have used Reichert Ultramictrotomes for the last 21 years,
} and now they have become Leica, but I was wondering what opinions that
} people have other other ultramicrotomes from other manufacturers, especially
} new ultramicrotomes, such as the Powertome X or XO from RMC Products. Are
} there other ultramicrotomes on the market that I should know about?
}
} This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
}



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 06:32:55 2005



From: ech :      ech-at-interchange.ubc.ca
Date: Thu, 07 Apr 2005 04:48:53 -0700 (PDT)
Subject: [Microscopy] Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Garry
we have three OM3s, an Ultracut E and an Ultracut T, all Leica. For the International Cryo EM Course last year, Leica brought in the UC6 which we got to thoroughly test. We really liked it.

They have made so many improvements on it that we (by that I actually mean George Postuma, Philip Hyam and Paul de George) easily had the course participants cutting cryo sections in a very short time. Three UC6's were bought by new faculty at UBC for their own labs in the next year and I believe them to be satisfied customers. These don't have cryo capability; they use our cryo system if they need it. Since we have George at the cryo course, we have been using the Tokuyasu method in our lab a lot more. He makes it very easy. The advantages of course being that the immunogold penetrates the whole section as there is no resin to get in the way. We used to have a lot of trouble with this method until George came from Jan Slot's lab and gave us his experience.

We have cryo cutting ability on the Ultracut E, but the ease of use of the Ultracut E and UC6 is night and day. Leica are bringing UC6s to the cryo course this year in June. One thing we hope to try is high pressure freezing, cryo planing (UC6) and transfering onto the cryo stage of the cryo sem. We will also be trying the new Bal-Tec cryo transfer device to go from the high pressure freezer to the cryo-sem. I haven't advertised the cryo course on the website this year as there are only two places left. This year Helmut Gnaegi from Diatome will also be coming to teach cutting techniques.

I am sorry that I have no experience of the other products that you mentioned. Will you be going to the Microscopy Society of Canada meeting in Hamilton in May? Maybe we can touch base then as I would love to network and find out about these other products.

As you are in Canada, I guess Philip Hyam is your Leica rep too. We have always had very good dealings with him. We have someone in Vancouver who is very good at servicing our microtomes which is an added benefit and probably the most important thing about buying a new piece of equipment. At least for me running a facility.
Elaine
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} I am interested in purchasing a new Ultramicrotome for sectioning in
} diagnostic pathology to replace an aging Reichert that has been giving us
} some problems. I have used Reichert Ultramictrotomes for the last 21 years,
} and now they have become Leica, but I was wondering what opinions that
} people have other other ultramicrotomes from other manufacturers, especially
} new ultramicrotomes, such as the Powertome X or XO from RMC Products. Are
} there other ultramicrotomes on the market that I should know about?
}
} This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
}
--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 07:29:48 2005



From: Richard Edelmann :      edelmare-at-MUOhio.edu
Date: Thu, 7 Apr 2005 08:44:57 -0400
Subject: [Microscopy] Re: Trade names: was: "Dualbeam" "Crossbeam"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

With respect to Charles and a few other vendors, I agree with Larry and
John. A Unique name is very valuable and can serve a company exceedingly
well in recognition - and justifyable should be protected legally. If the name
comes be associated with a particular product or even better to be almost
synonymous with technique or product so much the better for the vendor - as
in Kleenex, or EDAX. But like “Miracle Tip” forceps, you will note that these
are unique names and NOT simply descriptive terms. Attempting to force
synonymy by trademarking or registering a description is “questionable” to
say the least. However, we at least have EDS, EDX, EDXS, XEDS, or even
EDXRF under which to list the technique used.

What I was hoping for was an answer from the folks who are actually
USING “dual column ,dual gun, sample milling and imaging FIB/SEM systems
“ on a term they use or even would LIKE to use for the technique. I have no
vested interest in the technology, I do not use the instruments, nor even write
about them, but in trying to assemble the buyers guide for the EXPO
supplement for this summers M&M meeting I just wanted a name to list the
vendors under rather than leaving the “products” out of the listing all together.


However, it has turned into an interesting discussion and I do hope the
vendors are listening. Secondly, it does raise the point that as a society of
real users perhaps we should be the ones whom tell the vendors what to call
things.

(With some apologies to the marketing folks at FEI and Zeiss for offending
or even EDAX)


} }
} } Folks:
} }
} } Help! I need a different name to call "Dualbeam" or "Crossbeam"
} } instruments since one is trademarked and the other is registered (FEI &
} } Zeiss).
} }
} }
} }
} } Richard E. Edelmann, Ph.D.
}
} Although I do have a vested interest, quite honestly I can't see that
} 'legal' protection for these terms achieves anything for anybody
} apart from feeding the lawyers. In both cases, they are hardly more
} than a simple description.
}
} Personally, I'd suggest everybody use both as much as possible to
} emphasise what nonsense it is.
} --
} Larry Stoter
} JEOL (UK) Ltd
} tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com
}
} PLEASE NOTE
} 1. Any mail other than plain text will be automatically deleted.
} 2. Any mail, legitimate or not, apparently or actually from hotmail,
} netscape, yahoo or excite will automatically be deleted.
} 3. Mail with no subject or without a clear subject will be ignored :-)
}



Richard E. Edelmann, Ph.D.
Associate Editor of EXPO, Microscopy and Microanalysis Supplement
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 07:45:34 2005



From: Lesley Graham :      patljg-at-gwumc.edu
Date: Thu, 07 Apr 2005 09:00:50 -0400
Subject: [Microscopy] fibrin embedded cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Name: Lesley Graham

Organization: George Washington Univ.

Has anyone ever embedded cells in fibrin as opposed to agar? If so,
what protocol did you use?

Thank you,
Lesley


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 08:23:02 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 07 Apr 2005 09:38:14 -0400
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debby,
You may be making for some very unhappy cells by removing the FBS a
day ahead of fixation. Yes, you want to remove it so that it does
not get fixed to the surface of your cells, but I have found that
washing the cells with serum-free media 2-3 times just prior to
fixation is usually adequate and that way the cells are kept in their
"happy" conditions right up to the end.
When you say that the coversilps are cell culture treated, what does
that mean? Poly-l-lysine? Collagen? A different base might work
better. Caco-2 cells are big ugly things, but they are widely used so
you should be able to find references for conditions for good
adherence.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 08:33:25 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Apr 2005 08:48:35 -0500
Subject: [Microscopy] HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

We also have this microscope and we can see 10nm immunogold label in
backscatter mode, so you should get much better than that in SE. For
highest resolution, we use the shortest working distances possible, high
KV's, and keep the stage lock on. We normally just use high-resolution
mode, but sometimes we manually set the condenser lenses to higher
values. Often, however, by doing this the image may become so noisy
that the increased resolution doesn't do any good, since you can't
really see it anyway. At close working distances we make sure the lower
detector is "off", since it contributes little or nothing to the signal.


The lower the noise level in the room, the better. Keep very quiet
during high-mag image recording, since we can often see noise vibration
from voices in the picture. Try to keep your vacuum pumps and air
compressor in another room, if possible. If that's not possible, try
to reduce vibrations from them by setting pumps on short lengths of
thick vacuum hose. Make sure the nitrogen dewar for the cold finger is
full and give it time to settle down---it will contribute significant
vibration for up to 15 minutes after filling.

For surface resolution on "softer", i.e., less dense samples, such as
most biological specimens, it may be better to use lower accelerating
voltages to limit generation of SE's from deeper inside the specimen.
This will increase contrast of surface features, which is not the same
as resolution, and will help keep them from being overwhelmed by
secondaries originating from deeper inside the sample.

One thing we have consistently noted in our instrument is that very
close working distances require more or less constant realignment of the
scope, including electronic aperture alignment and stigmation. We check
alignment at the highest mag possible for each and every image we
record. I don't know if this is a characteristic of these scopes, in
general, but it is certainly the case for us. A little error in
stigmation can really mess up an image at high magnification.

Use the lightest coatings of the finest grain metals possible. We
routinely use platinum to coat our samples, and for high-resolution work
we start with a light coat (say 10mA for 15-30 seconds in our coater)
and add more metal as needed until we get charging under control. You
can always add more, but removing a too-heavy layer is another story.
Chromium coating is another option for even finer grain, but the
oxidation problem limits the useful lifetime of a single coating. Or
one can carbon coat.

We find that every sample is unique, especially when trying for the
highest resolutions we can get. We often check several accelerating
voltages.

I am sure that other users of this scope have their own tricks and tips
for high-res work and Dr. David Joy and others teach a short course on
how to maximize the potential of these instruments.

We love this scope! It requires tweaking to get the best results, but
those results can be spectacular.

Hope this helps a little.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
} From: yezer-at-cc.hut.fi [mailto:yezer-at-cc.hut.fi]
Sent: Thursday, April 07, 2005 4:51 AM
To: Microscopy-at-microscopy.com

Hi all,

I am using Hitachi S-4700 FESEM (cold tip) and so far didn't succeed to
achieve a SE image with resolution better than 10 nm (including in the
ultra high- resolution mode). Can anyone help us with a guide lines how
to reach high resolution by controlling the parameters (Ie, Cond lens,
and etc) with this microscope?.

Thanks,

E. Yossef
Helsinki University of Technology
Physical Metallurgy and Materials Science




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 09:03:49 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Thu, 07 Apr 2005 09:19:01 -0500
Subject: [Microscopy] Re: Re: RE: Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

getting back to the original issue, which was raised by larry stoter, i
believe.

for those of us who also work with molecular biology, there is a
prominant example of the importance of trade names and what can happen
if we fail to protect them. i refer to the TaqMan assay system. i
suspect most of us have heard of TaqMan, and 'the TaqMan machine'. this
system for (RT-)PCR, which utilizes 5' nuclease sensitive
oligonucleotides for probes, was developed by Perkin Elmer and used
prominantly in marketing and use of their 7100 realtime thermocycler
system. the way i understand the story, several years ago a competitor,
Roche, realized that the name had not been trademarked. they registered
the name. technically, now you can only use the name when you are
referring to realtime (RT)-PCR using a Roche system, while those who
popularized the concept have been left out in the cold.

we should all remember this type of story when the issue of trade names
arises, along with the consequences of failure to adequately protect
them. personally, i view the merchandising of scientific discoveries
with a great deal of distaste, and from the notes suspect larry stoter
has a similar view. but the issue of recognition is important. we need
to know that our peers know what we do and recognize us. it is
important in our scientific careers. we depend upon protection through
referencing our publications, copyright protection against plagerism,
and legal protection against theft of intellectual property. like it or
not, tradenames are as important to the business community as our
publications and recognition guaranteed by copyrights are to us.

note, i have no interest in any companies, molecular, EM, or otherwise,
but have used 5'-nuclease probes with various systems. to avoid any
dispute and try to be fair to all, i make it clear that i refer to the
system as "5'-nuclease probe" regardless of what method or
manufacturer's system i am using to test for a molecular target.

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 09:39:11 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Thu, 07 Apr 2005 09:54:08 -0500
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debby,

First, try fixing with 1.25% glut + 1% tannic acid. This should
improve the cells' structures, especially the membrane. Also, when we
do SEM of cultured cells, we typically don't use OsO4. No need, and
it can interfere with visualizing colloidal metal labels with BSE
imaging. When I have used OsO4 with cells, I again add 1% tannic acid
to the OsO4.
I'm not surprised the cells stick better to glass, though. They may
stick even better to gold. If you have a 100% Au target in your
sputter coater, or gold wire for your vacuum evaporator, you might
try gold-coating the glass or Thermanox and then growing the cells.
Caco-2 ... the only time I've done these was using transwells in
multi-well plates, 1.25% glut in serum (and other protein) free
buffer (organics free is better, like Na/Na2 PO4, as the organics
tend to fix into an obscuring goo over the cells), no OsO4. The
Caco-2 cells stuck to the transwell membranes very nicely. Given the
way OsO4 can harden cells and tissues, this may be your problem.
These wells also allow experiments where the cells have different
environments on the two surfaces (basal and lumenal). Give the
transwells a try.

Phil
P.S. When you "critical pint dry", what pint do you use? I tend to
prefer Elijah Craig.


} Hi all,
}
} I have a student who is trying to grow cultured cells on coverslips to use
} them for SEM imaging. However, the cells either do not stay on the
} coverslips when they are transported to our lab or tend to lift during
} preparation for SEM. Growth conditions, etc are below.
}
} Cell media: Dulbecco's Mod. Eagle Medium, 4.5g/L glucose, Na pyruvate, L-
} glutamine. The cells are grown in the presence of 10% FBS, but the FBS is
} removed from the media the day before the experiment.
}
} Coverslips: Thermanox plastic coverslips, cell culture treated on one side,
} purchased from VWR. Glass coverslips have also been tried and actually
} worked better than the thermanox.
}
} Cells: Caco-2 cells, grown for 8-10 days prior to conducting experiment.
}
} Fixation is with 3% glutaraldehyde in phosphate buffer followed by 1%
} osmium, ETOH series and critical pint dry. Samples are handled very gently
} when solutions are changed.
}
} We usually have little trouble with cultured cells but this one has been
} nothing but problems. Suggestions for growth or SEM prep would be
} appreciated.
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 09:59:15 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Thu, 07 Apr 2005 10:14:57 -0500
Subject: [Microscopy] Re: sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gordon,

I find that with our Balzers MED 010, which is basically the same
thing, I have to have the pressure at 2X10^-5 mbar to 1X10^-1 mbar
routinely, as was given this value by the good folks at TechnoTrade.
As to arcing and never getting the plasma to ignite, the only times
I've had this happen, it was cured by opening up the chamber and lid
and cleaning everything, including under the sputtering target.
Especially around the target.

Phil

} Hello,
} Since the topic of sputter coating has come up, I had a question I
} was wondering about. We have a Balzers/technotrade MED020 sputter
} coater. When coating, sometimes I have trouble generating the
} plasma. It will go through arcing. If I adjust the argon flow to
} give a good amount of argon in the chamber I can usually get the
} plasma to ignite better and have more stability, then I can turn the
} argon flow down. Sometimes though, the plasma will not ignite at
} all. I'm stumped by the problem and was wondering if anyone had
} some advice?
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak
} Electron Microscope Lab
} AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 10:01:26 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 07 Apr 2005 11:10:18 -0400
Subject: [Microscopy] Re: Re: Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, not everyone has had bad experiences with Ann Korsen.
She has brought us instruments on loan last fall, and was most
helpful and generous towards us, as well as easily reachable
at all times. Overall my experience with Leica has always been
most pleasant. Yes, they do offer small rebates on instruments,
but in the end you get what you pay for! All the experts in the
field of cryo-ultramicrotomy will tell you that there is only one
machine on the market worth considering. I experienced this
first-hand at a EMBO course in Paris last September, when
we had the choice between Leica Ultracut microtomes and
RMC. Yes, maybe RMC works well when you know how to
use it, but there was no doubt in my mind (and that of the other
instructors on the course) that the Leica machine is beautifully
designed, user-friendly and reliable, at least as far as cryo-sectioning
is concerned. So OK, one company will cost much more and
give smaller rebates, but if you had the choice between a
Yugo with a 25% rebate, or a Mercedes with no rebate, and
money was not an issue, what would you buy?!!
And like you, I don't have any special interests in either
company. I'm just a very happy Leica customer!

Marc


On Wednesday, April 6, 2005, at 09:29 PM, Sergey Ryazantsev wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} I had terrible experience with Ann Korsen - she basically did not
} returned telephone calls and ignored my E.mails. At one single
} instance when I had a chance to talk to her, she was quite helpless
} and rigid. I am sorry, this is my personal experience on the way to
} purpose new ultratome. I also had multiple problems a couple years
} ago when my UCT needed service. Finally, some problems were resolved
} (not in my favor to be truthful, on some I just gave up) but I still
} have a feeling that Leica's personnel was sort of unfriendly to me.
} To me, Leica provides miserable 5% "educational" discount (as a huge
} favor to me), they do not exchange/return stuff at all: illuminator I
} ordered for UC6 appeared to be for UCT and they denied to exchange
} (unopened box) or get it back. Spare-parts box for UCT was broken, it
} took more than year for Leica to replace it. Knifemaker was at least a
} month late than promised... In my last purpose, they were trying to
} "sweet" my experience with Leica including some free stuff. Paul
} DeGeorge was quite nice after all, I appreciate it. Nevertheless,
} having terrible customer service (from my experience), they have
} superior instruments. I also have to admit that I never ever had any
} negative experience (only positive) with people from RMC - they are
} extremely helpful and flexible. I don't have any interest in Leica or
} RMC: I am using the products from both companies. Sergey
}
} At 01:35 PM 4/6/2005, you wrote:
}
}
} } ----------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ---------
} }
} } HI, Garry,
} }
} } Leica, which is the company which acquired the Reichert microtome
} } business as part of the Cambridge-Leitz merger in 1990, came out last
} } year with the UC6. I had a chance to see it "up close and personal"
} } at Cell Bio last December and to work with it a little as part of a
} } new AFM/ultramicrotome integration for both bio and polymer
} } applications. The same people who sold your Reichert (ex: Ann
} } Korsen) are with Leica and can answer your questions. Ann can be
} } reached at Akorsen-at-leica-microsystems.com
} }
} } Hope this is helpful.
} }
} } Best regards,
} } Barbara Foster
} }
} } Microscopy/Microscopy Education
} } 313 S Jupiter Rd, Suite 100
} } Allen, TX 75002
} } P: 972-954-8011
} } W: www.MicroscopyEducation.com
} }
} } P. S.
} } Need a good general reference or light microscopy text? Call us today
} } to learn more about "Optimizing LIght Microscopy". Copies still
} } available through MME... even for class-room lots ... and we give
} } quantity discounts.
} }
} }
} }
} } At 01:58 PM 4/6/2005, Garry Burgess wrote:
} }
} }
} } } ---------------------------------------------------------------------
} } } ---------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ---------------------------------------------------------------------
} } } ----------
} } }
} } }
} } } I am interested in purchasing a new Ultramicrotome for sectioning in
} } } diagnostic pathology to replace an aging Reichert that has been
} } } giving us
} } } some problems. I have used Reichert Ultramictrotomes for the last
} } } 21 years,
} } } and now they have become Leica, but I was wondering what opinions
} } } that
} } } people have other other ultramicrotomes from other manufacturers,
} } } especially
} } } new ultramicrotomes, such as the Powertome X or XO from RMC
} } } Products. Are
} } } there other ultramicrotomes on the market that I should know about?
} } }
} } } This e-mail and/or any documents in this transmission is intended
} } } for the address(s) only and may contain legally privileged or
} } } confidential information. Any unauthorized use, disclosure,
} } } distribution, copying or dissemination is strictly prohibited. If
} } } you receive this transmission in error, please notify the sender
} } } immediately and return the original.
} }
} }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} 10833 Le Conte Ave, Room 33-080
} Los Angeles, CA 90095
}
} Phone: (310) 825-1144 (office)
} (310) 206-1029 (Lab)
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 10:13:21 2005



From: Lesley Weston :      leswes-at-shaw.ca
Date: Thu, 07 Apr 2005 08:28:27 -0700
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Would it be possible for the student to glow-discharge the coverslips
(either glass or Thermanox) before plating the cells? This would make them
much more adhesive and has the side-benefit of sterilising them as well. It
could affect the behaviour of the cells, but this might not matter,
depending on the experiment. Another possibility is to remove the FBS from
the medium closer to the time of the experiment, if possible. The presence
or absence of FBS has large effects on cell behaviour, possibly including
adhesion.

Lesley Weston.


} From: Debby Sherman {dsherman-at-purdue.edu}
} Date: Wed, 06 Apr 2005 21:47:16 -0500
} To: "message to: MSA list" {microscopy-at-MSA.microscopy.com}
} Subject: [Microscopy] Cultured cell prep for SEM
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
--} -
}
} Hi all,
}
} I have a student who is trying to grow cultured cells on coverslips to use
} them for SEM imaging. However, the cells either do not stay on the
} coverslips when they are transported to our lab or tend to lift during
} preparation for SEM. Growth conditions, etc are below.
}
} Cell media: Dulbecco's Mod. Eagle Medium, 4.5g/L glucose, Na pyruvate, L-
} glutamine. The cells are grown in the presence of 10% FBS, but the FBS is
} removed from the media the day before the experiment.
}
} Coverslips: Thermanox plastic coverslips, cell culture treated on one side,
} purchased from VWR. Glass coverslips have also been tried and actually
} worked better than the thermanox.
}
} Cells: Caco-2 cells, grown for 8-10 days prior to conducting experiment.
}
} Fixation is with 3% glutaraldehyde in phosphate buffer followed by 1%
} osmium, ETOH series and critical pint dry. Samples are handled very gently
} when solutions are changed.
}
} We usually have little trouble with cultured cells but this one has been
} nothing but problems. Suggestions for growth or SEM prep would be
} appreciated.
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 10:22:17 2005



From: Robert A Underwood :      underwoo-at-u.washington.edu
Date: Thu, 7 Apr 2005 08:37:47 -0700 (PDT)
Subject: [Microscopy] picking up ultrathin cryosections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Fellow Microscopists,

I have a question about picking up ultrathin cryosections. I have notice when I am using very lightly
fixed tissue, many of the sections look like the tissue is stretching apart, leaving small holes
throughout. I thought at first that this was just due to lack of fixation, however I could find a ocassional
section/grid that was amazingly better. This indicated that the pickup is a major factor. I have tried
sucrose vs. methylcellulose/sucrose vs. methylcellulose/sucrose/UA and find that it is still just in the
pickup that some are better than others. Do you have some advice on how to pickup the sections to
avoid this type of artifact?
Thank you for any advice.

Robert Underwood
Research Scientist
University of Washington
Seattle, WA USA







From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 11:08:53 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 07 Apr 2005 12:24:01 -0400
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

another nice thing about Transwells (for TEM): if you cut the filter
from the holder before dehydration, when you put them into Proplyene
oxide they curl up like a jelly roll. You can then embed them in a
flat mold and cut cross sections of the roll so that you get many
more cell profiles/section than if you'd cut a strip of the membrane
and just embedded that.
For SEM, I leave them intact and put them into the CPD. You just
have to keep track of which one is which.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 11:13:28 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Thu, 07 Apr 2005 12:28:18 -0400
Subject: [Microscopy] Re: fibrin embedded cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We were using this technique around 1970 when I was a grad student. It was
published by a guy named J. Furtado and it may have been published in
MYCOLOGIA. That is the best I can do for you with out some more digging.
It worked quite nicely to keep fungal spores in place during
ptocessing.

Good luck on finding it and let me know.

Greg

At 09:00 AM 4/7/2005, Lesley Graham wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 11:26:35 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Apr 2005 11:41:50 -0500
Subject: [Microscopy] HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In my earlier reply to this, I forgot to mention one other factor that
affects the final quality of the digital image produced by our S4700. A
peculiarity of our instrument, and I have seen at least one other
mention of this on the listserv, is that when an image from the scope is
opened in Adobe Photoshop it comes up in "Indexed Color" mode. I have
no idea why. If this image is converted into 8-bit greyscale mode it
gets cleaned up quite a bit in terms of "noise"---the difference is
sometimes striking.

Granted this has nothing to due with the resolution of the image in the
microscope, but it does have a lot to do with the appearance of the
final presentation image.

Is anyone else familiar with this?

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
} From: yezer-at-cc.hut.fi [mailto:yezer-at-cc.hut.fi]
Sent: Thursday, April 07, 2005 4:51 AM
To: Microscopy-at-microscopy.com

Hi all,

I am using Hitachi S-4700 FESEM (cold tip) and so far didn't succeed to
achieve a SE image with resolution better than 10 nm (including in the
ultra high- resolution mode). Can anyone help us with a guide lines how
to reach high resolution by controlling the parameters (Ie, Cond lens,
and etc) with this microscope?.

Thanks,

E. Yossef
Helsinki University of Technology
Physical Metallurgy and Materials Science




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 11:37:16 2005



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Thu, 7 Apr 2005 12:52:07 -0700
Subject: [Microscopy] Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HI, Debby-
I don't know why anyone would want to use cover slips. We grow them
on the regular plastic dishes they are used to. These samples are
easy to embed and the cell layer separates readily from the dish if
the procedure is performed correctly. If yu give me a FAX number, I
shall send you a protocol This one is not on our web site (yet).
Carol


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu
http://www.bgsu.edu/departments/biology/facilities/MnM
___________________________________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 12:35:58 2005



From: pgrover :      pgrover-at-bilbo.bio.purdue.edu
Date: Thu, 7 Apr 2005 12:51:57 -0500
Subject: [Microscopy] re: Fun stories about EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm sure I'm not alone in my fond memories of the cool micrographs that used
to grace the cover of the Journal of Irreproducible Results (and maybe I'm
not the only one to drop my subscription when they stopped doing it). Does
anyone know whether there exists a compendium of those cover images? Or any
other collection of weird & wonderful micrographs?

Paul Grover

----------------------------------------------------------------------
"Keep your eyes slightly wide and blank. Show no interest or excitement."
- Dr. Miles Bennell
Invasion of the Body Snatchers (1956)

(Opinions expressed here are not necessarily those of my employer or of
Purdue University)








From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 12:51:18 2005



From: Peter Ingram :      p.ingram-at-voice.cellbio.duke.edu
Date: Thu, 7 Apr 2005 14:07:01 -0400
Subject: [Microscopy] Trademarks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below you will find a short comment from a lawyer on
trademarks, copyrights and patents that might clarify - or confuse! -
the issue. In any event if there is enough interest I am sure the
MSA Public Policy committee could organize a discussion on trademarks
at the Hawaii meeting this August.

If nyone is interested, please respond to me with the word
"Trademaks" in the Subject line so that a) the Listserv and b) my
regular email don't get unecessarily cluttered!

Peter Ingram

MSA Legal Liaison

Comments:

The two (trademarks and copyrights) are wholly different species of
intellectual property, and both are distinct from patents. Each is
governed by statute, but copyright and trademark law also has a large
add-on of "common law" arising out of both state statute and decided
cases. An overview/introduction to the
three categories of intellectual property staes that there are actually "3.5
categories" - the ".5" is a very different, but very interesting, area
of "trade secrets." These are largely non-statutory and, most
interesting, based on exactly the opposite legal basis of the other
three - that is, copyright, trademark and patent laws depend on public
disclosure, publicity, to establish their strengths - patents, as you
know, mandate full public disclosure, in filings with the US Patent
office, which are publicly available - in order for the patent to
"issue." The reason for this is that patent protection is limited in
time; after the patent time expires, all are free to exactly duplicate
the instructions in the patent. Trademarks are not similarly limited in
time, but may be lost by failure to protect them adequately
--
Peter Ingram
Duke University Medical Center
Box 90319
LaSalle Street Extension
DURHAM NC USA 27708-0319

Tel: (919) 660-2695
Fax: (919) 660-2671
e-mail: p.ingram-at-cellbio.duke.edu



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 12:56:48 2005



From: Paul Webster :      pwebster-at-hei.org
Date: Thu, 07 Apr 2005 11:10:06 -0700
Subject: [Microscopy] Re: picking up ultrathin cryosections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Robert,

You have come across an interesting observation with cryosections. As you
know cryosections are not embedded in a continuous, polymerized plastic
sheet, as are resin-embedded sections. The only physical bonds holding the
structure together are those protein interactions that may remain after the
tissue has been treated, and cross-linking bonds formed by the aldehyde
fixation. Of course, less fixation will mean fewer cross-linking bonds to
hold the sections together.

To cut the story short, the variability you are seeing in the section
morphology is most probably due to surface tension effects on the surface of
the pick-up drop. Reduce the surface tension and you will improve the
section morphology.

Warm pick-up solutions will spread the sections more than frozen drops of
pick-up solution. More methyl cellulose in the sucrose appears also to
reduce the surface tension. You could also try adding gelatin to the sucrose
to lower the surface tension.

You could also experiment with the time taken to collect the sections from
the knife. You may find that as you get better at picking them up, the
morphology will get worse. If this is so, slow down the process and give the
pick-up solution time to cool down. Once you get a good result, stick with
the protocol.

Alternatively, you could add a very small amount of glutaraldehyde to your
fixative. This may toughen up the sections enough to improve the morphology.

Finally, make sure that you are not drying the sections during the labeling
process. If you are in the habit of taking off excess buffer before you
float that grids on antibody you may be drying them enough to cause
morphology changes. Thin sections are very sensitive to drying.

I am sure that you are aware that you can take your frozen, cryoprotected
blocks and either warm them up again for embedding in epoxy resin. This has
always been a good way to convince people that poor fixation or freezing is
not the reason for poor morphology in cryoprotected frozen material.

You can also take the frozen blocks of sucrose-infiltrated material and
freeze substitute in cold methanol. This is also a good way of checking the
morphology if the material is then embedded in epoxy resin.

Regards,

Paul Webster.




Paul Webster, Ph.D.
House Ear Institute
2100 West Third Street
Los Angeles
CA 90057-1922



On 4/7/05 8:37 AM, "Robert A Underwood" {underwoo-at-u.washington.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Hi Fellow Microscopists,
}
} I have a question about picking up ultrathin cryosections. I have notice when
} I am using very lightly
} fixed tissue, many of the sections look like the tissue is stretching apart,
} leaving small holes
} throughout. I thought at first that this was just due to lack of fixation,
} however I could find a ocassional
} section/grid that was amazingly better. This indicated that the pickup is a
} major factor. I have tried
} sucrose vs. methylcellulose/sucrose vs. methylcellulose/sucrose/UA and find
} that it is still just in the
} pickup that some are better than others. Do you have some advice on how to
} pickup the sections to
} avoid this type of artifact?
} Thank you for any advice.
}
} Robert Underwood
} Research Scientist
} University of Washington
} Seattle, WA USA
}
}
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 13:01:45 2005



From: Jay Campbell :      microtomy-at-gmail.com
Date: Thu, 7 Apr 2005 13:16:30 -0500
Subject: [Microscopy] Re: Re: Re: Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

"Yes, maybe RMC works well when you know how to
use it"

In my experience this is pretty important no matter what
brand/piece of equipment you are talking about. Some people have a
preference based on what they are "used to" that may have little or no
bearing on the quality of the competing product.
Jay

On Apr 7, 2005 10:10 AM, Marc Pypaert {marc.pypaert-at-yale.edu} wrote:
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Well, not everyone has had bad experiences with Ann Korsen.
} She has brought us instruments on loan last fall, and was most
} helpful and generous towards us, as well as easily reachable
} at all times. Overall my experience with Leica has always been
} most pleasant. Yes, they do offer small rebates on instruments,
} but in the end you get what you pay for! All the experts in the
} field of cryo-ultramicrotomy will tell you that there is only one
} machine on the market worth considering. I experienced this
} first-hand at a EMBO course in Paris last September, when
} we had the choice between Leica Ultracut microtomes and
} RMC. Yes, maybe RMC works well when you know how to
} use it, but there was no doubt in my mind (and that of the other
} instructors on the course) that the Leica machine is beautifully
} designed, user-friendly and reliable, at least as far as cryo-sectioning
} is concerned. So OK, one company will cost much more and
} give smaller rebates, but if you had the choice between a
} Yugo with a 25% rebate, or a Mercedes with no rebate, and
} money was not an issue, what would you buy?!!
} And like you, I don't have any special interests in either
} company. I'm just a very happy Leica customer!
}
} Marc
}
}
} On Wednesday, April 6, 2005, at 09:29 PM, Sergey Ryazantsev wrote:
}
} }
} }
} } -----------------------------------------------------------------------
} } -------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------
} } --------
} }
} } I had terrible experience with Ann Korsen - she basically did not
} } returned telephone calls and ignored my E.mails. At one single
} } instance when I had a chance to talk to her, she was quite helpless
} } and rigid. I am sorry, this is my personal experience on the way to
} } purpose new ultratome. I also had multiple problems a couple years
} } ago when my UCT needed service. Finally, some problems were resolved
} } (not in my favor to be truthful, on some I just gave up) but I still
} } have a feeling that Leica's personnel was sort of unfriendly to me.
} } To me, Leica provides miserable 5% "educational" discount (as a huge
} } favor to me), they do not exchange/return stuff at all: illuminator I
} } ordered for UC6 appeared to be for UCT and they denied to exchange
} } (unopened box) or get it back. Spare-parts box for UCT was broken, it
} } took more than year for Leica to replace it. Knifemaker was at least a
} } month late than promised... In my last purpose, they were trying to
} } "sweet" my experience with Leica including some free stuff. Paul
} } DeGeorge was quite nice after all, I appreciate it. Nevertheless,
} } having terrible customer service (from my experience), they have
} } superior instruments. I also have to admit that I never ever had any
} } negative experience (only positive) with people from RMC - they are
} } extremely helpful and flexible. I don't have any interest in Leica or
} } RMC: I am using the products from both companies. Sergey
} }
} } At 01:35 PM 4/6/2005, you wrote:
} }
} }
} } } ----------------------------------------------------------------------
} } } --------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------
} } } ---------
} } }
} } } HI, Garry,
} } }
} } } Leica, which is the company which acquired the Reichert microtome
} } } business as part of the Cambridge-Leitz merger in 1990, came out last
} } } year with the UC6. I had a chance to see it "up close and personal"
} } } at Cell Bio last December and to work with it a little as part of a
} } } new AFM/ultramicrotome integration for both bio and polymer
} } } applications. The same people who sold your Reichert (ex: Ann
} } } Korsen) are with Leica and can answer your questions. Ann can be
} } } reached at Akorsen-at-leica-microsystems.com
} } }
} } } Hope this is helpful.
} } }
} } } Best regards,
} } } Barbara Foster
} } }
} } } Microscopy/Microscopy Education
} } } 313 S Jupiter Rd, Suite 100
} } } Allen, TX 75002
} } } P: 972-954-8011
} } } W: www.MicroscopyEducation.com
} } }
} } } P. S.
} } } Need a good general reference or light microscopy text? Call us today
} } } to learn more about "Optimizing LIght Microscopy". Copies still
} } } available through MME... even for class-room lots ... and we give
} } } quantity discounts.
} } }
} } }
} } }
} } } At 01:58 PM 4/6/2005, Garry Burgess wrote:
} } }
} } }
} } } } ---------------------------------------------------------------------
} } } } ---------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } } America
} } } } To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } } ---------------------------------------------------------------------
} } } } ----------
} } } }
} } } }
} } } } I am interested in purchasing a new Ultramicrotome for sectioning in
} } } } diagnostic pathology to replace an aging Reichert that has been
} } } } giving us
} } } } some problems. I have used Reichert Ultramictrotomes for the last
} } } } 21 years,
} } } } and now they have become Leica, but I was wondering what opinions
} } } } that
} } } } people have other other ultramicrotomes from other manufacturers,
} } } } especially
} } } } new ultramicrotomes, such as the Powertome X or XO from RMC
} } } } Products. Are
} } } } there other ultramicrotomes on the market that I should know about?
} } } }
} } } } This e-mail and/or any documents in this transmission is intended
} } } } for the address(s) only and may contain legally privileged or
} } } } confidential information. Any unauthorized use, disclosure,
} } } } distribution, copying or dissemination is strictly prohibited. If
} } } } you receive this transmission in error, please notify the sender
} } } } immediately and return the original.
} } }
} } }
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } 10833 Le Conte Ave, Room 33-080
} } Los Angeles, CA 90095
} }
} } Phone: (310) 825-1144 (office)
} } (310) 206-1029 (Lab)
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }
} }
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 13:21:10 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Thu, 07 Apr 2005 14:35:57 -0400
Subject: [Microscopy] Re: picking up ultrathin cryosections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If it makes you feel better, most of us are struggling with the
very same problem! So I am very interested in the responses
you are going to receive to your question.

I used to think that when picking up, either with sucrose alone
or sucrose/methyl cellulose, one had to be extremely quick, and
the sections had to magically lift up onto the still liquid drop in
which it would disappear as if they were dissolving in the sucrose.
But talking to others since then, I have come to realize that
being too fast can actually harm the sections, by over-stretching
them and making holes and tears appear. One person told me
that she waits until the rim of the loop of sucrose/methylcellulose
just starts freezing (a white ring appearing at the periphery of
the droplet) before picking up the sections. Given the speed
at which sucrose/methylcellulose freezes, this often means that
by the time the sections have been picked up and the loop
retrieved from the chamber, the whole drop is now frozen. You
just have to wait for it to thaw again, then transfer the sections on
the grid. But this technique hasn't worked too well in my hands
so far - I get too many folds. There are too many factors that
are involved here: size of the loop (we use a very small one -
smaller diameter than a grid), percentage of sucrose and methyl
cellulose (I use 50/50), temperature of the chamber (-108°C
as opposed to -120°C), the use of gelatin to embed samples,
and of course the fixation protocol. But anyway, you should
maybe give this a try and see if waiting a few more sections
during pick up, so that the surface of your drop is close to
freezing point, might help prevent some of the tearing.

By the way, what do you call "very slightly fixed", and why
do you have to fix so lightly?

Best

Marc


On Thursday, April 7, 2005, at 11:37 AM, Robert A Underwood wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Hi Fellow Microscopists,
}
} I have a question about picking up ultrathin cryosections. I have
} notice when I am using very lightly fixed tissue, many of the sections
} look like the tissue is stretching apart, leaving small holes
} throughout. I thought at first that this was just due to lack of
} fixation, however I could find a ocassional section/grid that was
} amazingly better. This indicated that the pickup is a major factor. I
} have tried sucrose vs. methylcellulose/sucrose vs.
} methylcellulose/sucrose/UA and find that it is still just in the
} pickup that some are better than others. Do you have some advice on
} how to pickup the sections to avoid this type of artifact?
} Thank you for any advice.
}
} Robert Underwood
} Research Scientist
} University of Washington
} Seattle, WA USA
}
}
}
}
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 13:29:05 2005



From: bbandli :      bbandli-at-mvainc.com
Date: Thu, 07 Apr 2005 14:44:41 -0400
Subject: [Microscopy] Re: RE: HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our JEOL6500-F (JEOL PCSEM software) has the same indexed color
"feature" and converting it to 8-bit greyscale has the same effect of
cleaning up the noise in the image. I also have no idea why.

Bryan Bandli

Tindall, Randy D. wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 14:29:40 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Apr 2005 14:45:26 -0500
Subject: [Microscopy] Re: Re: New Ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I feel I need to chime in on this, since my experiences with Leica have
been wonderful. I have dealt with Ann Korsen, Paul DeGeorge, Robert
Seiler, and others in the company in both sales and training situations
and have no complaints about their knowledge, willingness to work with
the customer, or followup on any problems that might occur. This has
also been true of their distributors, in our case Mager Scientific.

That said, I have also had very pleasant dealings with the RMC crew
during demonstrations and workshops. If I were buying a new
ultramicritome I would be making my equipment decisions based solely on
the equipment itself and the applications it will address, since both
companies field good people, in my opinion. Maybe I'm just
wishy-washy....

Our lab and I have no financial or other ties with any of these
companies, etc., but we do have two Leica UCT ultramicrotomes (one with
cryo), a Leica high-pressure freezer, and a Leica freeze-substitution
unit, so we have plenty of experience with this company.

Just my two copper-coated zinc plugs worth....

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu




-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Wednesday, April 06, 2005 8:29 PM
To: Microscopy-at-microscopy.com

I had terrible experience with Ann Korsen - she basically did not
returned telephone calls and ignored my E.mails. At one single instance
when I had a chance to talk to her, she was quite helpless and rigid. I
am sorry, this is my personal experience on the way to purpose new
ultratome. I also had multiple problems a couple years ago when my UCT
needed service. Finally, some problems were resolved (not in my favor
to be truthful, on some I just gave up) but I still have a feeling that
Leica's personnel was sort of unfriendly to me. To me, Leica provides
miserable 5% "educational" discount (as a huge favor to me), they do not
exchange/return stuff at all: illuminator I ordered for UC6 appeared to
be for UCT and they denied to exchange (unopened box) or get it back.
Spare-parts box for UCT was broken, it took more than year for Leica to
replace it. Knifemaker was at least a month late than promised... In my
last purpose, they were trying to "sweet" my experience with Leica
including some free stuff. Paul DeGeorge was quite nice after all, I
appreciate it. Nevertheless, having terrible customer service (from my
experience), they have superior instruments. I also have to admit that
I never ever had any negative experience (only positive) with people
from RMC - they are extremely helpful and flexible. I don't have any
interest in Leica or RMC: I am using the products from both companies.
Sergey

At 01:35 PM 4/6/2005, you wrote:


} -----------------------------------------------------------------------
} ------- The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver

} Cell Bio last December and to work with it a little as part of a new
} AFM/ultramicrotome integration for both bio and polymer applications.
} The same people who sold your Reichert (ex: Ann Korsen) are with Leica
} and can answer your questions. Ann can be reached at
} Akorsen-at-leica-microsystems.com
}
} Hope this is helpful.
}
} Best regards,
} Barbara Foster
}
} Microscopy/Microscopy Education
} 313 S Jupiter Rd, Suite 100
} Allen, TX 75002
} P: 972-954-8011
} W: www.MicroscopyEducation.com
}
} P. S.
} Need a good general reference or light microscopy text? Call us today
} to learn more about "Optimizing LIght Microscopy". Copies still
} available through MME... even for class-room lots ... and we give
quantity discounts.
}
}
}
} At 01:58 PM 4/6/2005, Garry Burgess wrote:
}
}
} } ----------------------------------------------------------------------
} } -------- The Microscopy ListServer -- Sponsor: The Microscopy Society

} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver

} } us some problems. I have used Reichert Ultramictrotomes for the last
} } 21 years, and now they have become Leica, but I was wondering what
} } opinions that people have other other ultramicrotomes from other
} } manufacturers, especially new ultramicrotomes, such as the Powertome X

} } or XO from RMC Products. Are there other ultramicrotomes on the
market that I should know about?
} }
} } This e-mail and/or any documents in this transmission is intended for
} } the
} } address(s) only and may contain legally privileged or confidential
} } information. Any unauthorized use, disclosure, distribution, copying
} } or dissemination is strictly prohibited. If you receive this
} } transmission in error, please notify the sender immediately and return
the original.
}
}

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu







From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 15:17:34 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 08 Apr 2005 08:33:45 +1200
Subject: [Microscopy] EDS recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The best and simple thing to do is to go to the nearest business centre and
tell them that you want to register trademark on your product. They will
teach you what to do, how much it may cost, and how to do this properly.
Even they will recommend to you how to do this, and what start from, and you
will have an idea how much it will cost. They will refer you to appropriate
lawyers, and organization. Trademarks are associated with commercial, or
ready to sell products. Do you have it? If you have commercial product you
may wish to sell it by your own company. Then you may decide to register
your company, and name of your product could be associated with name of the
company. Just some ideas.
Victoria


----- Original Message -----
} From: "Peter Ingram" {p.ingram-at-voice.cellbio.duke.edu}
To: {microscopy-at-MSA.Microscopy.com}
Sent: Thursday, April 07, 2005 11:07 AM

Hi

I'm in the market for a new LN2-cooled, Be-window Si(Li) EDS detector, to go on a
JEOL 840, and to be used mainly for quantitative analysis of minerals.

The manufacturers that I'm currently considering are EDAX, Gresham, Thermo Noran,
and Rontec.

Anybody got any to add to this list?

Anybody got any reasonably valid bias towards or against any of these?

Confidentiality of off-list replies will be respected.

Please note that I'm looking for a detector/preamp only, I already have a very
satisfactory pulse-processor, mca, etc.


cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 7 18:16:26 2005



From: Aleksandr Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Fri, 08 Apr 2005 09:37:16 +0100
Subject: [Microscopy] Re: picking up ultrathin cryosections?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for the replies so far, keep 'em coming, but please note that I'm enquiring about
detectors only ie detector plus detector preamp, not systems (pulse-processor, MCA,
software etc).

cheers

rtch


} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
Organization: Dept of Geology, Univ of Auckland
To: microscopy-at-microscopy.com
Date sent: Fri, 08 Apr 2005 08:33:45 +1200

Dear Robert,

1) It would be better to use tissues fixed with a little percentage of
glutaraldehyde (0.1-0.2%). this will not kill much of antigenic epitops
but will greatly improve the ultrastructural stability of the sample.
You can find exact reference in the papers of P.J.Peters group in
Amsterdam or H.Geuse group in Utrecht.
2) In my hands the best solution for picking up is
Methylcellulose/sucrose. Sucrose alone has a very big surface tension,
which will eventually destroy sensitive structures as Golgi complex.
Addition of UA to the pick up solution can improve the ultrastructure
but can reduce labeling. You can try to add low concentration of tannic
acid (less than 0.1%) in solution. Sometimes it helps but be aware of
the effects on the epitopes for labeling.
3) The other thing to consider is timing of your pick up. It is very
critical to pick up at the moment when the solution just on the verge to
start to freeze. If you will pick up sections sooner or later then you
will probably end up with a horrible ultrastructure. It is very hard to
explain how to determine the right moment. I think it will be better
that you just try yourself several settings because our instuments for
pick up could be different from yours (loop diameter, wire thickness
etc.) and it will affect the timing. May be this movie from P.Peters'
lab will help:
http://129.112.18.40/cryomovies/

Sincerely,
Alex

Robert A Underwood:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Hi Fellow Microscopists,
}
} I have a question about picking up ultrathin cryosections. I have
} notice when I am using very lightly fixed tissue, many of the sections
} look like the tissue is stretching apart, leaving small holes
} throughout. I thought at first that this was just due to lack of
} fixation, however I could find a ocassional section/grid that was
} amazingly better. This indicated that the pickup is a major factor. I
} have tried sucrose vs. methylcellulose/sucrose vs.
} methylcellulose/sucrose/UA and find that it is still just in the
} pickup that some are better than others. Do you have some advice on
} how to pickup the sections to avoid this type of artifact?
} Thank you for any advice.
}
} Robert Underwood
} Research Scientist
} University of Washington
} Seattle, WA USA
}
}
}
}
}
}
}

--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 04:31:43 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Fri, 8 Apr 2005 07:17:00 -0230
Subject: [Microscopy] RE: RE: HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy D. writes ...

} ... A peculiarity of our instrument, and I have seen at
} least one other mention of this on the listserv, is that
} when an image from the scope is opened in Adobe Photoshop
} it comes up in "Indexed Color" mode. I have no idea why.
} If this image is converted into 8-bit greyscale mode it
} gets cleaned up quite a bit in terms of "noise"---

Check this behaviour at Photoshop 100% magnification and above. I believe
that Photoshop blends pixels differently at lower view magnifications,
depending on whether the working space is color managed or not. An indexed
grayscale would not be color managed.

The better question would be to ask the manufacturuers for their reasons
why grayscales are indexed. Most do, and there is really no justification.

my $0.02 & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 07:09:35 2005



From: cbutterick-at-poco.com (by way of MicroscopyListserver)
Date: Fri, 8 Apr 2005 07:25:41 -0500
Subject: [Microscopy] viaWWW: Independent Service Contractor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cbutterick-at-poco.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, April 7, 2005 at 09:21:35
---------------------------------------------------------------------------

Email: cbutterick-at-poco.com
Name: Chuck Butterick

Organization: Poco Graphite, Inc

Title-Subject: [Microscopy] [Filtered] Independent Service Contract

Question: Is there an independent service engineer interested in servicing a DSM 962 (currently under contract) in Texas (DFW)?

Contact me off the Listserver, please.


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 07:10:15 2005



From: mcary-at-gatan.com (by way of MicroscopyListserver)
Date: Fri, 8 Apr 2005 07:26:06 -0500
Subject: [Microscopy] viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mcary-at-gatan.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, April 7, 2005 at 12:12:38
---------------------------------------------------------------------------

Email: mcary-at-gatan.com
Name: Matthew Cary

Organization: gatan inc

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: how do i most acuratly measure the ohms/square inch of an indium tin coated surface??

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 07:10:07 2005



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Fri, 08 Apr 2005 09:25:38 -0300
Subject: [Microscopy] Re: RE: RE: HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The same thing happens with the images from our JEOL JSM-5600. A real
annoyance since a lot of useful
things in Photoshop are disabled for indexed images. I usually convert
them wholesale to grayscale with a
Photoshop script. In the case of the 5600, I believe the images are
indexed so that you can apply the
color lookup table, etc. to the images within the GUI. A relatively
useless "eye candy" feature, in my
opinion, but then I'm not a salesman. Equally annoying is that the color
stuff that you can draw on the images
(measurement lines, etc.) are smashed down into grayscale when they are
written into the image! Why aren't
these colors preserved in the indexed image? Grrr! Anybody interested in
starting a thread about useless
and annoying "features" on scopes that could best be left out or
improved? Maybe the manufacturers are listening....

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf



michael shaffer wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 08:43:49 2005



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Fri, 8 Apr 2005 10:24:57 -0400
Subject: [Microscopy] re new anything

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Yee Yan,

My understanding is that there are several factors involved in selecting
an accelerating voltage (KV). At higher KVs, chromatic aberration is
reduced, resulting in greater resolution, i.e., the ability to separate
two points visually. However, higher KVs also result in the generation
of secondary electrons from a larger surface area, due to primary beam
electron scattering over a larger lateral distance, as well as from
deeper within the specimen.

This theoretically has two effects. One is that the larger area of the
surface emitting SE's gives a larger "effective spot size", or area from
which signal is generated, and this decreases resolution. The other is
that the SEs escaping from deeper within the specimen are detected along
with the SEs generated at or just below the surface, and this part of
the signal can obscure surface detail by lowering contrast. This effect
can easily be seen by taking a biological specimen or other soft sample
and simply comparing a 1.0 KV image to a 30 KV image: the higher KV
image looks smoother and less detailed.

This effect is complicated by the effect of sample density, in that a
denser sample will have a smaller "effective spot size" than a
less-dense sample at the same KV. This means that for many samples you
can increase resolution by increasing KV if the sample is dense enough
to restrict the electron scattering to acceptable levels for the
magnification you are working at. In addition, higher KVs generally
allow such adjustments as using smaller apertures to decrease spherical
aberration effects and increase depth of field, and higher lens
currents to demagnify the probe size and increase resolution that way.
Generally speaking, adjusting the SEM for high resolution means
decreasing the signal to the detectors, so if you have more signal to
begin with, you can afford to lose more signal before noise overwhelms
your image.

I agree with you that for many samples, especially biological ones,
lower KVs are better at retaining surface detail in the image. We
routinely run our FESEM at 5.0 KV, and often at 1.0 KV. But not always.
Separating out the relative effects of larger "effective spot sizes"
(decreased resolution), escape of SE's from deeper within the specimen
(lower surface contrast), and decreased aberrations (increased
resolution) at high KV's is not straightforward. And let's not even get
into the effects of coatings on these factors, since you may need
heavier coatings to counter increased charging effects, as you
mentioned! As I said, it's probably best to approach each sample as
unique and experiment.

By copy of this to the listserv I am asking to be set straight on any
errors in the above sermon. I always learn a lot from these discussions
and have had faulty assumptions corrected on several occasions.

Cheers,
Randy

-----Original Message-----
} From: Tay Yee Yan [mailto:one_twinklestar-at-yahoo.com.sg]
Sent: Thursday, April 07, 2005 7:17 PM
To: Tindall, Randy D.

I have been getting increasingly disturbed by the extensive traffic
concerning both ultramicrotomes and personnel associated with companies
selling the product. Some observations after roughly 30 years in the
business of purchasing and maintaining both major instruments (TEM, SEM,
XPS, Auger, EPMA, SIMS, etc) and ancillary equipment (microtomes, polishers,
ion mills, etc):

1. for a given instrument most everyone with extensive experience possesses
'brand loyalty' to some degree, based on a combination of need,vs instrument
specs, a good price, reasonable expectation of good service and
user-friendlyness over a period of time.

2. even if the above has held true for some time, you never know when new
developments may occur, pricing policies may change (or can be forced to
change on a case-by-case basis), or sales and service personnel might change
(don't forget the former people as they often can influence the latter).
It's an ever-shifting field.

3. returning to #1, you never know when your needs may change, so be careful
about swearing too much loyalty to a particular brand (unless you have a
very stable capital budget and senior management that trusts you
completely!).

4. I've known a lot of company staff from sales, service and/or R&D, and
have met relatively few 'duds'. These people work very hard and in a
surprising number of cases are paid even less than we are. For certain they
have nowhere near our degree of autonomy, so please keep their names (pro or
con) off the air. I doubt any of us would appreciate our qualifications
and style being debated in public.

5. For that matter, why not simply keep specific product names off the
Listserver as much as possible? Give your candid impressions to the
inquirer off-line. If you feel the need to enlighten the Listserver at
large, confine your comments to more generic comments, "I find in my lab hat
a good X needs to have qualities A, B, C-- to get the job done".

6. As an example of #5, I have given some dozen workshops on hard materials
microtomy with both of the producers mentioned in the above thread.
Students have attended who have, or are considering, instruments from both
vendors. The focus has religously been on the technique and its potential
results, not the pros and cons of the products that can get you there, and
the results have been very pleasing to all concerned. In my opinion, this
is the prime purpose of the Listserver; education, not brand endorsement.

Tom

Dr. Tom Malis
Manager
Academic User Access Facility (AUAF)
CANMET Materials Technology Laboratory
Natural Resources Canada
558 Booth St., Ottawa, Ontario K1A 0G1
Tel.: (613) 995-7358 FAX: (613) 992-8735, cell: 613-371-4577
malis-at-nrcan.gc.ca {mailto:malis-at-nrcan.gc.ca}




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 09:43:18 2005



From: Linda Fox :      lfox1-at-lumc.edu
Date: Fri, 08 Apr 2005 09:58:20 -0500
Subject: [Microscopy] TEM digital plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Friends,

Is anyone using digital plates in place of film in their TEM's?

Can you let me know about cost, efficiency, resolution, practicality,
and any problems that you have had. Also what system you are using and
has the service been good.

We are looking into this option as one way to "go digital" with 2 old
TEM's. Also, does anyone still use film, but then scan the images into
a computer? That is another option that we are looking at.
Thanks,
Linda Fox
lfox1-at-lumc.edu

Linda M. Fox
Loyola University
Stritch School of Medicine
Core Imaging Facility
2160 S. First Ave.
Maywood, Il 60153
Bld. 102 Room 0617
1-708-216-3395
lfox1-at-lumc.edu



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 09:45:57 2005



From: Linda Fox :      lfox1-at-lumc.edu
Date: Fri, 08 Apr 2005 10:01:01 -0500
Subject: [Microscopy] Video Camera for LM scope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are upgrading our image analysis system (Leitz Orthoplan interfaced to an Apple computer running OSX and ImageJ) and would appreciate any suggestions for a video camera with a screw mount. We currently have an older Javelin with a ½ inch chip, which has a thread mount on a tube (approx 7/8 in diameter) out the back port. We'd like to keep the tube so we don't need to worry about the optical path, working distance, etc.
Thanks,
John McNulty
jmcnulty-at-lumc.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 11:35:58 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 8 Apr 2005 10:07:04 -0700
Subject: [Microscopy] Re: viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 8, 2005, at 5:26 AM, by way of MicroscopyListserver wrote:

} Question: how do i most acuratly measure the ohms/square inch of an
} indium tin coated surface??
}
Dear Matthew,
There are two papers by Mike Lamvik (RS Rader & MK Lamvik (1992)
High-conductivity amorphous TiSi substrates for low-temperature
electron microscopy. J. Microsc. 168, 71-77. and MK Lamvik, SD
Davilla, and J Tuttle (1989) Properties of substrates for low
temperature quantitative microscopy and microanalysis. Scan. Microsc.
Suppl. 3 271-276.) that describe resistivity measurements of thin
films. If you can configure your coating as described in those papers,
that would be a way to measure the resistance accurately. If, however,
your coating is already prepared, and if it is not on an insulating
surface, you will have to go to greater lengths, and accurate
measurement may not be possible. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 11:37:49 2005



From: William H Roberts :      William.H.Roberts-at-USA.dupont.com
Date: Fri, 8 Apr 2005 12:53:10 -0400
Subject: [Microscopy] viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Matthew,

Surface resistivity has units of ohms per square (not per square inch). I
know that this sounds strange, but the dimensions drop out in the
calculations. There are a number of vendors who sell the circular
concentric electrodes which are used to measure the value, for example:
keithly and trek, to name just two. Also if you just Google on "surface
resistivity measurement" there are some very good papers that are available
in pdf format which will explain surface resistivity in much greater detail
than I can. There may even be some simple way of measuring the value
without the use of one of the commercial units (or without making one
yourself).

Bill




mcary-at-gatan.com (by way of MicroscopyListserver) on 04/08/2005 08:26:06 AM


To: microscopy-at-microscopy.com
cc:


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mcary-at-gatan.com) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday,
April 7, 2005 at 12:12:38
---------------------------------------------------------------------------

Email: mcary-at-gatan.com
Name: Matthew Cary

Organization: gatan inc

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: how do i most acuratly measure the ohms/square inch of an indium
tin coated surface??

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From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 12:24:25 2005



From: Gerroir, Paul :      paul.gerroir-at-xrcc.xeroxlabs.com
Date: Fri, 8 Apr 2005 13:38:35 -0400
Subject: [Microscopy] re new anything

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom,
Well said. Unfortunately there have been individuals who use this
listserver to make personal attacks publicly. Ultimately they are the
ones who suffer.

Paul


Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: 905-823-7091, ext.216
FAX: 905-822-7022
e-mail: paul.gerroir-at-xrcc.xeroxlabs.com

-----Original Message-----
} From: Malis, Tom [mailto:malis-at-NRCan.gc.ca]
Sent: Friday, April 08, 2005 10:25 AM
To: 'microscopy-at-microscopy.com'

I have been getting increasingly disturbed by the extensive traffic
concerning both ultramicrotomes and personnel associated with companies
selling the product. Some observations after roughly 30 years in the
business of purchasing and maintaining both major instruments (TEM, SEM,
XPS, Auger, EPMA, SIMS, etc) and ancillary equipment (microtomes,
polishers, ion mills, etc):

1. for a given instrument most everyone with extensive experience
possesses 'brand loyalty' to some degree, based on a combination of
need,vs instrument specs, a good price, reasonable expectation of good
service and user-friendlyness over a period of time.

2. even if the above has held true for some time, you never know when
new developments may occur, pricing policies may change (or can be
forced to change on a case-by-case basis), or sales and service
personnel might change (don't forget the former people as they often can
influence the latter).
It's an ever-shifting field.

3. returning to #1, you never know when your needs may change, so be
careful about swearing too much loyalty to a particular brand (unless
you have a very stable capital budget and senior management that trusts
you completely!).

4. I've known a lot of company staff from sales, service and/or R&D, and
have met relatively few 'duds'. These people work very hard and in a
surprising number of cases are paid even less than we are. For certain
they have nowhere near our degree of autonomy, so please keep their
names (pro or
con) off the air. I doubt any of us would appreciate our
qualifications and style being debated in public.

5. For that matter, why not simply keep specific product names off the
Listserver as much as possible? Give your candid impressions to the
inquirer off-line. If you feel the need to enlighten the Listserver at
large, confine your comments to more generic comments, "I find in my lab
hat a good X needs to have qualities A, B, C-- to get the job done".

6. As an example of #5, I have given some dozen workshops on hard
materials microtomy with both of the producers mentioned in the above
thread.
Students have attended who have, or are considering, instruments from
both vendors. The focus has religously been on the technique and its
potential results, not the pros and cons of the products that can get
you there, and the results have been very pleasing to all concerned. In
my opinion, this is the prime purpose of the Listserver; education, not
brand endorsement.

Tom

Dr. Tom Malis
Manager
Academic User Access Facility (AUAF)
CANMET Materials Technology Laboratory
Natural Resources Canada
558 Booth St., Ottawa, Ontario K1A 0G1
Tel.: (613) 995-7358 FAX: (613) 992-8735, cell: 613-371-4577
malis-at-nrcan.gc.ca {mailto:malis-at-nrcan.gc.ca}






From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 12:39:03 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 8 Apr 2005 11:10:09 -0700
Subject: [Microscopy] Re: TEM digital plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 8, 2005, at 7:58 AM, Linda Fox wrote:

} Is anyone using digital plates in place of film in their TEM's?
}
} Can you let me know about cost, efficiency, resolution, practicality,
} and any problems that you have had. Also what system you are using and
} has the service been good.
}
} We are looking into this option as one way to "go digital" with 2 old
} TEM's. Also, does anyone still use film, but then scan the images into
} a computer? That is another option that we are looking at.
}
Dear Linda,
Although I have not used imaging plates myself, I did investigate them
at one time. Their resolution and linearity are excellent, and their
quantum efficiency (the fraction of electrons that result in a signal)
is the best of any detector system I know of for ~100 kV electrons.
This falls off with increasing energy, however, and they are not so
good at 300-400 kV (let alone at 1.2 MV, which I was interested in).
I'm sure that the properties are likely to have improved since I looked
into things. The cost of the plates themselves is not an issue, since
they are reusable, and the cost of the reader was on the order of
$100,000. I expect that, if the readers have improved, the cost has
risen commensurably, but I am not up to date on this. As far as film
is concerned, we are still investigating whether film or CCD will
ultimately be better for single-particle analysis. The benefits of
film are the larger field of view and (for ED measurements) the fact
that one does not have to insert a beam stop over the unscattered beam.
The disadvantages of film are its limited linearity and the necessity
to scan it in order to use computer processing, either of which will
introduce errors into quantitation. It is also more difficult to get
the same consistency in the developing process as is inherent in a CCD
image. Additionally, to get good quantitation requires a good scanner,
and for ED, one needs something like the Perkin-Elmer microdensitometer
(at a cost of a few hundred thousand dollars), since one needs to get
accurate readings from small areas of high OD surrounded by large areas
of essentially transparent film. In other words, the best system
depends on just what kinds of experiments one is doing (and might be
doing in the future).
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 12:48:59 2005



From: Roger Baker :      rcbaker-at-eden.infohwy.com
Date: Fri, 8 Apr 2005 13:03:34 -0500
Subject: [Microscopy] Re: viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One way to do the job is to paint two low resistance parallel lines on
the surface with a conductive silver particle-based paint (carbon based
conductive paint probably has too much resistance) and then use a good
digital ohmmeter to measure the resistance across the gap. The
resistance in of such an oxide film might be typically be on the order
of 10-100 ohms per square.

You might want to put a straight sliver of masking tape two
millimeters wide down first and then paint over it in a region 1 cm
long with the conductive paint. Since the painted conductors are 1 cm
long with a separation of 2 mm, this equals .2 square (once you peel
away the masking tape). You would then multiply the measured resistance
by five to get the correct value in ohms per square. -- Roger



}
} Email: mcary-at-gatan.com
} Name: Matthew Cary
}
} Organization: gatan inc
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: how do i most acuratly measure the ohms/square inch of an
} indium tin coated surface??
}
} -----------------------------------------------------------------------
} ----
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 13:18:24 2005



From: Scott Walck on Comcast :      swalck-at-comcast.net
Date: Fri, 8 Apr 2005 14:30:43 -0400
Subject: [Microscopy] viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sheet resistance is measured by a 4-point probe. The probes are equidistance
(about 1 mm) and inline. A constant current is put between the outer two
and the voltage drop between the inner two is measured. Then the sheet
resistance is given by V/I except that there is a geometric correction
factor (CF) to convert the voltage/current ratio measured by the 4-point
probe into sheet resistance. This correction factor accounts for the sample
size, shape and probe spacings. The probes are spring mounted to avoid
damaging the film. The sheet resistance measure by the probe is given by:

Rs = (V/I) * CF.

For an semi-infinite thin sheet, CF = 4.53. What is typically done is to
set the current at 0.453 mA and then multiply the voltage measured by 10.
The bulk resistivity of the coating material is given as Rs * t.

The measured resistance of a sample of dimensions L and W and sheet
resistance, Rs is given by
R = Rs * W/L. Which means that if you have a square sample of any size, the
resistance will be the same. An easy way to measure the sheet resistance
fairly accurately on transparent conducting coated glass samples is to take
a square sample of about 12 x 12 inches and measure the resistance with a
multimeter across it. If you know the sheet resistance of a coating, then
all you do is "count the squares". For example if you have a sample that is
twice its length, then the resistance along the long axis will be twice the
sheet resistance because it is two squares by one square, but across it, it
will be 1/2 because it is only a half square across.

There are commercially available four point probes for sheet resistance
available. I think that Keithley makes one. You might also consider this
source: http://bridgetec.com/srm.html
I Don't have any financial interest in either of these companies and I am
sure that they are not the only manufacturers of four point probes.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127





-----Original Message-----
} From: by way of MicroscopyListserver [mailto:mcary-at-gatan.com]
Sent: Friday, April 08, 2005 8:26 AM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mcary-at-gatan.com) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday,
April 7, 2005 at 12:12:38
---------------------------------------------------------------------------

Email: mcary-at-gatan.com
Name: Matthew Cary

Organization: gatan inc

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: how do i most acuratly measure the ohms/square inch of an indium
tin coated surface??

---------------------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 13:35:30 2005



From: Valery Ray :      vray-at-partbeamsystech.com
Date: Fri, 8 Apr 2005 11:50:48 -0700 (PDT)
Subject: [Microscopy] Re: Re: viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Matthew,

In addition to the papers mentioned by Bill, you can
order a little book called "Low Level Measurements"
from Keithley Instruments, (www dot keithley dot com)
- it is a free literature.

On the pages 4-27 through 4-31 of this book is a
descripton of four-probe method for measurements of
surface resistivity, including some tricks for
enhancing accuracy.

Disclaimer: PBS&T has no financial interest in
Keithley Instruments, just a satisfied user.


--- Bill Tivol {tivol-at-caltech.edu} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
}
} On Apr 8, 2005, at 5:26 AM, by way of
} MicroscopyListserver wrote:
}
} } Question: how do i most acuratly measure the
} ohms/square inch of an
} } indium tin coated surface??
} }
} Dear Matthew,
} There are two papers by Mike Lamvik (RS Rader & MK
} Lamvik (1992)
} High-conductivity amorphous TiSi substrates for
} low-temperature
} electron microscopy. J. Microsc. 168, 71-77. and MK
} Lamvik, SD
} Davilla, and J Tuttle (1989) Properties of
} substrates for low
} temperature quantitative microscopy and
} microanalysis. Scan. Microsc.
} Suppl. 3 271-276.) that describe resistivity
} measurements of thin
} films. If you can configure your coating as
} described in those papers,
} that would be a way to measure the resistance
} accurately. If, however,
} your coating is already prepared, and if it is not
} on an insulating
} surface, you will have to go to greater lengths, and
} accurate
} measurement may not be possible. Good luck.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}
}

Valery Ray

Particle Beam Systems
& Technology
www.partbeamsystech.com


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 14:15:15 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 08 Apr 2005 12:30:19 -0700
Subject: [Microscopy] Re: viaWWW: ohms/square

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The "best" way to measure sheet resistivity is with a
four point probe using the van der Pauw method.
you can read about it here:

http://electron.mit.edu/~gsteele/vanderpauw/

The measured area must be on an insulator. Sheet rho is
in units of Ohms/square. However, if you did a square inch,
then you would have Ohms/square inch.

There are other structures that can be made that are still
four points. One such structure is a long (relatively) runner
with contacts at each end and two contacts placed inwards from
each end. Make the whole thing an nice number of squares (1x1u,
2x2u, 5x5u, etc.) and make the inner two taps at another nice
number of squares. Run current through the outer contacts and
measure the voltage across the inner contacts.

In all of these measurements, contact resistance must be kept
low or results are off.

gary g.



At 05:26 AM 4/8/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 14:25:22 2005



From: John Twilley :      jtwilley-at-sprynet.com
Date: Fri, 08 Apr 2005 17:46:57 -0400
Subject: [Microscopy] RE: re new anything, public critique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

Just a small correction, the secondary electrons from the backscattered
electrons coming back out of the sample are still being generated and
escaping close to the surface. It is the size of the interaction volume of
the primary electrons, loosing some energy deeper in and then returning
back towards the surface and generating secondaries there that decrease the
resolution. Because of the more forward scattering of electrons and
increased backscattered near the surface on tilted surface, that the
resolution is further degraded on surface not perpendicular to the beam.

With respect to heavier coatings, what is important is to match the coating
with the need and sample. If the bulk conductivity of the sample is so low
that it can trap charge within it, then a high accelerating voltage can
still charge a sample that has a heavy conductive coating because the charge
can't escape to the surface. What is best is to have a conductive coating
that is thin and uniform, i.e. continuous, across the surface and use a beam
energy that will allow the charge to drain from the surface. This requires
both rotating the sample and tilting it doing coating. Also remember that
even if you coat your sample, you still need for the charge to drain to
ground. The coating itself should not interfere with the imaging. The
material type and deposition method affects the grain size and if it is too
big for the magnification that you want to image, then that's what you are
going to see, not the sample.


-Scott

Disclaimer: South Bay Technology, Inc. manufacturers and sells the IBS/e
sputter coater for high resolution coatings.

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127


-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Friday, April 08, 2005 9:59 AM
To: Tay Yee Yan
Cc: microscopy-at-microscopy.com

Hi Yee Yan,

My understanding is that there are several factors involved in selecting
an accelerating voltage (KV). At higher KVs, chromatic aberration is
reduced, resulting in greater resolution, i.e., the ability to separate
two points visually. However, higher KVs also result in the generation
of secondary electrons from a larger surface area, due to primary beam
electron scattering over a larger lateral distance, as well as from
deeper within the specimen.

This theoretically has two effects. One is that the larger area of the
surface emitting SE's gives a larger "effective spot size", or area from
which signal is generated, and this decreases resolution. The other is
that the SEs escaping from deeper within the specimen are detected along
with the SEs generated at or just below the surface, and this part of
the signal can obscure surface detail by lowering contrast. This effect
can easily be seen by taking a biological specimen or other soft sample
and simply comparing a 1.0 KV image to a 30 KV image: the higher KV
image looks smoother and less detailed.

This effect is complicated by the effect of sample density, in that a
denser sample will have a smaller "effective spot size" than a
less-dense sample at the same KV. This means that for many samples you
can increase resolution by increasing KV if the sample is dense enough
to restrict the electron scattering to acceptable levels for the
magnification you are working at. In addition, higher KVs generally
allow such adjustments as using smaller apertures to decrease spherical
aberration effects and increase depth of field, and higher lens
currents to demagnify the probe size and increase resolution that way.
Generally speaking, adjusting the SEM for high resolution means
decreasing the signal to the detectors, so if you have more signal to
begin with, you can afford to lose more signal before noise overwhelms
your image.

I agree with you that for many samples, especially biological ones,
lower KVs are better at retaining surface detail in the image. We
routinely run our FESEM at 5.0 KV, and often at 1.0 KV. But not always.
Separating out the relative effects of larger "effective spot sizes"
(decreased resolution), escape of SE's from deeper within the specimen
(lower surface contrast), and decreased aberrations (increased
resolution) at high KV's is not straightforward. And let's not even get
into the effects of coatings on these factors, since you may need
heavier coatings to counter increased charging effects, as you
mentioned! As I said, it's probably best to approach each sample as
unique and experiment.

By copy of this to the listserv I am asking to be set straight on any
errors in the above sermon. I always learn a lot from these discussions
and have had faulty assumptions corrected on several occasions.

Cheers,
Randy

-----Original Message-----
} From: Tay Yee Yan [mailto:one_twinklestar-at-yahoo.com.sg]
Sent: Thursday, April 07, 2005 7:17 PM
To: Tindall, Randy D.

As someone who once made a very significant purchase from a major microscope
manufacturer and then received a condenser lens assembly that was missing an entire
lens element in its interior, only to encounter foot dragging and imperious
responses from the regional sales manager regarding getting it replaced, I think
there needs to be some room for factual critique that doesn't cross the bounds of
libel. Had I not been intimately familiar with the normal construction and
performance of the condenser, I would have a) suffered for ever after thinking that
we got what we paid for; or b) wasted days of my instititution's time getting to
the bottom of it. I don't know why U.S. researchers should feel the need to muzzle
themselves regarding any irresponsible behavior on the part of sales people or
unmet technical performance. After all, I would guess that a great many
microscopes are bought with tax dollars directly or indirectly.

John Twilley

Gerroir, Paul wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Tom,
} Well said. Unfortunately there have been individuals who use this
} listserver to make personal attacks publicly. Ultimately they are the
} ones who suffer.
}
} Paul
}
} Paul J. Gerroir
} Microscopy
} Materials Characterization
} Xerox Research Centre of Canada
} 2660 Speakman Drive
} Mississauga, Ontario L5K 2L1
}
} Phone: 905-823-7091, ext.216
} FAX: 905-822-7022
} e-mail: paul.gerroir-at-xrcc.xeroxlabs.com
}
} -----Original Message-----
} } From: Malis, Tom [mailto:malis-at-NRCan.gc.ca]
} Sent: Friday, April 08, 2005 10:25 AM
} To: 'microscopy-at-microscopy.com'
} Subject: [Microscopy] re new anything
}
} ------------------------------------------------------------------------
} ------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ------------------------------------------------------------------------
} -------
}
} I have been getting increasingly disturbed by the extensive traffic
} concerning both ultramicrotomes and personnel associated with companies
} selling the product. Some observations after roughly 30 years in the
} business of purchasing and maintaining both major instruments (TEM, SEM,
} XPS, Auger, EPMA, SIMS, etc) and ancillary equipment (microtomes,
} polishers, ion mills, etc):
}
} 1. for a given instrument most everyone with extensive experience
} possesses 'brand loyalty' to some degree, based on a combination of
} need,vs instrument specs, a good price, reasonable expectation of good
} service and user-friendlyness over a period of time.
}
} 2. even if the above has held true for some time, you never know when
} new developments may occur, pricing policies may change (or can be
} forced to change on a case-by-case basis), or sales and service
} personnel might change (don't forget the former people as they often can
} influence the latter).
} It's an ever-shifting field.
}
} 3. returning to #1, you never know when your needs may change, so be
} careful about swearing too much loyalty to a particular brand (unless
} you have a very stable capital budget and senior management that trusts
} you completely!).
}
} 4. I've known a lot of company staff from sales, service and/or R&D, and
} have met relatively few 'duds'. These people work very hard and in a
} surprising number of cases are paid even less than we are. For certain
} they have nowhere near our degree of autonomy, so please keep their
} names (pro or
} con) off the air. I doubt any of us would appreciate our
} qualifications and style being debated in public.
}
} 5. For that matter, why not simply keep specific product names off the
} Listserver as much as possible? Give your candid impressions to the
} inquirer off-line. If you feel the need to enlighten the Listserver at
} large, confine your comments to more generic comments, "I find in my lab
} hat a good X needs to have qualities A, B, C-- to get the job done".
}
} 6. As an example of #5, I have given some dozen workshops on hard
} materials microtomy with both of the producers mentioned in the above
} thread.
} Students have attended who have, or are considering, instruments from
} both vendors. The focus has religously been on the technique and its
} potential results, not the pros and cons of the products that can get
} you there, and the results have been very pleasing to all concerned. In
} my opinion, this is the prime purpose of the Listserver; education, not
} brand endorsement.
}
} Tom
}
} Dr. Tom Malis
} Manager
} Academic User Access Facility (AUAF)
} CANMET Materials Technology Laboratory
} Natural Resources Canada
} 558 Booth St., Ottawa, Ontario K1A 0G1
} Tel.: (613) 995-7358 FAX: (613) 992-8735, cell: 613-371-4577
} malis-at-nrcan.gc.ca {mailto:malis-at-nrcan.gc.ca}





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 17:13:48 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 8 Apr 2005 15:44:57 -0700
Subject: [Microscopy] Re: RE: re new anything, public critique

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Apr 8, 2005, at 2:46 PM, John Twilley wrote:

} As someone who once made a very significant purchase from a major
} microscope
} manufacturer and then received a condenser lens assembly that was
} missing an entire
} lens element in its interior, only to encounter foot dragging and
} imperious
} responses from the regional sales manager regarding getting it
} replaced, I think
} there needs to be some room for factual critique that doesn't cross
} the bounds of
} libel. Had I not been intimately familiar with the normal
} construction and
} performance of the condenser, I would have a) suffered for ever after
} thinking that
} we got what we paid for; or b) wasted days of my instititution's time
} getting to
} the bottom of it. I don't know why U.S. researchers should feel the
} need to muzzle
} themselves regarding any irresponsible behavior on the part of sales
} people or
} unmet technical performance. After all, I would guess that a great
} many
} microscopes are bought with tax dollars directly or indirectly.
}

} } Tom,
} } Well said. Unfortunately there have been individuals who use this
} } listserver to make personal attacks publicly. Ultimately they are the
} } ones who suffer.
} }
} } Paul


} } } 4. I've known a lot of company staff from sales, service and/or R&D,
} } } and
} } } have met relatively few 'duds'. These people work very hard and in a
} } } surprising number of cases are paid even less than we are. For
} } } certain
} } } they have nowhere near our degree of autonomy, so please keep their
} } } names (pro or
} } } con) off the air. I doubt any of us would appreciate our
} } } qualifications and style being debated in public.

Dear John,
Prompt, competent service is a key component of running any
complicated piece of equipment, so both positive and negative comments
regarding service experiences, IMHO, have a place on this list. These
can always be made without mentioning the name of the person involved,
and personal attacks--as opposed to opinions as to the level of
service--have no place here. Of course, the particularly competent
service or sales person would likely appreciate being mentioned by
name, and I think that there are circumstances where this is proper;
e.g., when someone either high up in a company or known to be in the
same area as the person who posted a question to the list has performed
particularly well. In that case, the poster would be likely to deal
with that same person.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 18:12:14 2005



From: Paul Webster :      pwebster-at-hei.org
Date: Fri, 08 Apr 2005 16:26:15 -0700
Subject: [Microscopy] Re: re new anything - long message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

First I would like to thank Tom Malis for bringing some fairness back onto
the listserver.

What we should be looking for here is educational material. If someone asks
for an opinion about what microtome to buy, then we can ask what
applications the machine is to be used for and what the prospective new
customer will expect of their machine. If the machine does not meet the
standards required of it, we should have enough knowledge to recognize this.

As an illustration, some years ago, I appealed to this list-server to help
me through a purchase of an SEM. As an electron microscopist who knew
nothing about SEM's, I mistakenly thought I would have some useful advice
given to me when I had to finally make a decision.

Instead I only received two, off-line replies that both basically told me to
buy one machine (both answers listed the same machine but neither said why I
should buy it). There was no reasoned discussion on-line about the different
features offered by the microscope companies and how they would be useful,
nor was there any advice on sputter coaters and other ancillary equipment.

Eventually I relied on books and advice from sales people to make my final
decision. It may not have been the best decision and I am sure that many
people would not agree with my choice but it fitted with what I needed at
the time. However, it would have been comforting to know a little more about
the differences (advantages/disadvantages) of cold cathode and Schottke
electron guns, or the usefulness of databases etc before purchasing.

As customers we need to know that machines will perform as they are
advertised. We also need to know what is essential on a machine and what we
can cut out if we have to cut costs. Also important is the quality of
service offered by a company both in skill and availability.

An an aside, I once had a service engineer tell me a machine wasn't working
because I didn't know how to use it properly. However, when I asked him to
show me, he admitted that he didn't know how to use it either. Should I have
expected him to know how to use it?

So, if anyone asks me for advice on which ultramicrotome to buy I will tell
them that all currently available machines will cut sections well - this is
true if the machine is made to specification. However, I also know that
older ultramicrotomes that have been around for many years also cut sections
well. My very first ultrathin cryosections were prepared using a Christensen
cryobox attached to a Sorvall MT2-B using a glass knife. The sections were
as good as any that can be made using new machines. However, these machines
are no-longer available and service is impossible.

Stretching the metaphor that was used in an earlier message, a Mercedes is
wonderful if you can afford to run it but why get one when it is only to be
used once a month to do the shopping? After all, if we all wanted "the best"
machines, surely we would all be using Macintosh computers.

More seriously, for the people wondering about which ultramicrotome to buy,
I would suggest first (if possible) taking a look at the machines at a trade
show. Don't expect the machine to be working, but you will get to see and
touch it. You will also meet some of the company people there who will give
you an insight on how the machine was developed and who uses it. Try to get
a list of customers from them and talk to the customers. Of course, most
customers will be partisan because they have to justify their purchase.
However, if you talk to enough people off the record, you will get a good
impression of the companies you will be dealing with.

The next step is to find a way to actually use a machine before you buy it.
Going to a working laboratory is the best way to do this so that you can see
how the machine performs in a real environment and see how the machine fits
your working methods. I once saw a well endowed lady try to use a machine
that required long arms to operate - it was obvious as soon as she sat down
in front of it that she would be unable to use it. This is something she
would not have discovered if she had to rely on the opinions of others.

If we assume that all machines will do the job they are supposed to do, then
the user interface is probably the most important part of any machine.
Evaluating the interface will be a personal process. For example, I think
that when I organize things everything is easily accessible. My staff, who
are all significantly shorter than me, think the place is a disaster when I
put things away because they can't reach most of the stuff. Of course, I
don't spend all my time in the lab so they are correct in their assessment.

Another good way of evaluating a machine, and especially an ultramicrotome,
is to use it on a teaching course. A three day workshop will reveal much
about how a machine works. Watching machines working for 10 days, being
operated by novices is especially interesting.

Marc Pypaert, in his message, mentions an EMBO course in Paris last year
where cryoultramicrotomes were installed in a crowded laboratory and left
working all day for 10 days. This is an extreme case of what we would expect
a machine to endure yet most of the machines endured it. I am sure that many
sales were made as a result of what the participants witnessed. However,
there have been many other courses where some machines have failed and
others have excelled. Such endurance cannot be the only way of evaluating a
machine. Remember that most courses are held away from the home base of the
manufacturer and machines have to be shipped in (at great cost) for use on
the course. Damning a machine for not working under such conditions is
unfair.

In conclusion, the sale of ultramicrotomes has become a hot topic and the
support for different companies is probably more political than it should
be. As Tom Malis says, the people involved with manufacture and sales are
people too and are trying to do their job. Sometimes what they want to do
and what they have to do are conflicting tasks. However, if service and
machines are substandard the company will suffer in the long term. This is
normal in the business environment.

We can also affect the way we are treated by manufacturing and sales people
by the way we interact with them. This too is normal because we are all
human.

Our job as customers is to find what works best for us using as much
knowledge as we can obtain. As advisors our role is to educate the
customers.

If you read this far, thank you for your time.

Regards,

Paul Webster.



Paul Webster, Ph.D.
House Ear Institute
2100 West Third Street
Los Angeles
CA 90057-1922
Phone: 213 273 8026
Fax: 213 13 739






On 4/8/05 7:24 AM, "Malis, Tom" {malis-at-nrcan.gc.ca} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} I have been getting increasingly disturbed by the extensive traffic
} concerning both ultramicrotomes and personnel associated with companies
} selling the product. Some observations after roughly 30 years in the
} business of purchasing and maintaining both major instruments (TEM, SEM,
} XPS, Auger, EPMA, SIMS, etc) and ancillary equipment (microtomes, polishers,
} ion mills, etc):
}
} 1. for a given instrument most everyone with extensive experience possesses
} 'brand loyalty' to some degree, based on a combination of need,vs instrument
} specs, a good price, reasonable expectation of good service and
} user-friendlyness over a period of time.
}
} 2. even if the above has held true for some time, you never know when new
} developments may occur, pricing policies may change (or can be forced to
} change on a case-by-case basis), or sales and service personnel might change
} (don't forget the former people as they often can influence the latter).
} It's an ever-shifting field.
}
} 3. returning to #1, you never know when your needs may change, so be careful
} about swearing too much loyalty to a particular brand (unless you have a
} very stable capital budget and senior management that trusts you
} completely!).
}
} 4. I've known a lot of company staff from sales, service and/or R&D, and
} have met relatively few 'duds'. These people work very hard and in a
} surprising number of cases are paid even less than we are. For certain they
} have nowhere near our degree of autonomy, so please keep their names (pro or
} con) off the air. I doubt any of us would appreciate our qualifications
} and style being debated in public.
}
} 5. For that matter, why not simply keep specific product names off the
} Listserver as much as possible? Give your candid impressions to the
} inquirer off-line. If you feel the need to enlighten the Listserver at
} large, confine your comments to more generic comments, "I find in my lab hat
} a good X needs to have qualities A, B, C-- to get the job done".
}
} 6. As an example of #5, I have given some dozen workshops on hard materials
} microtomy with both of the producers mentioned in the above thread.
} Students have attended who have, or are considering, instruments from both
} vendors. The focus has religously been on the technique and its potential
} results, not the pros and cons of the products that can get you there, and
} the results have been very pleasing to all concerned. In my opinion, this
} is the prime purpose of the Listserver; education, not brand endorsement.
}
} Tom
}
} Dr. Tom Malis
} Manager
} Academic User Access Facility (AUAF)
} CANMET Materials Technology Laboratory
} Natural Resources Canada
} 558 Booth St., Ottawa, Ontario K1A 0G1
} Tel.: (613) 995-7358 FAX: (613) 992-8735, cell: 613-371-4577
} malis-at-nrcan.gc.ca {mailto:malis-at-nrcan.gc.ca}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 18:13:38 2005



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Fri, 8 Apr 2005 19:28:09 -0700
Subject: [Microscopy] Fwd: Re: Cultured cell prep for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debby and LISTers
OOPS! I apologize. I thought you were asking for a TEM protocol - I
also have one for SEM. There is no need to reply. I shall FAX it to
you.
C.

} X-Authentication-Warning: ns.microscopy.com: mail set sender to
} MicroscopyL-request-at-ns.microscopy.com using -f
} X-Sender: heckman-at-mailstore.bgsu.edu
} Date: Thu, 7 Apr 2005 12:52:07 -0700
} To: {microscopy-at-MSA.microscopy.com} , Debby Sherman {dsherman-at-purdue.edu}
} From: Carol Heckman {heckman-at-bgnet.bgsu.edu}
} Subject: [Microscopy] Re: Cultured cell prep for SEM
} X-MASF: 0.00%
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu
http://www.bgsu.edu/departments/biology/facilities/MnM
___________________________________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 8 23:58:09 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 8 Apr 2005 22:12:58 -0700
Subject: [Microscopy] RE: Re: re new anything - long message (short response, though)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Uh, guys, well, thank you all for your opinions on how this listserver
ought to be run and what is appropriate to be on it, but ah, isn't all
that really up to Nestor, since he runs it (a great personal effort
perhaps not always sufficiently appreciated)?

John Mardinly
408-765-2346
877-277-1182





From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 00:30:33 2005



From: Alberto Diaspro :      diaspro-at-fisica.unige.it
Date: Sat, 9 Apr 2005 07:44:59 +0200
Subject: [Microscopy] reprint about Abbe's work et al.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear friends,
I'd appreciate very much if you you send me the pdf of the following
paper:
Applied Optics, Volume 5, Issue 11, 1720-
November 1966

Ernst Abbe and his work

H. Volkmann

and some other pdf or ppt related to Abbe's formula. IN Genoa, within
the Science Festival 2005, I am tentatively organizing an exhibition of
microscopes dedited to Abbe's work, celebrating again 1905-2005.
Thank you in advance for your help.
All my best
Alby

p.s. Science festival will be held in Genoa from October 25 to
Novembere 5, approx, see also at
http://www.festival.infm.it/it/home.php

------------------------------------------------------------------------
------------------------------------------------------------------------
--------------------------------------------------
[...] Dance with wolves and count the stars, including the
unseen...(L.Ferlinghetti, Challenges to young poet, 2001)
------------------------------------------------------------------------
------------------------------------------------------------------------
-------------------------------------------
Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department
of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309; URL:
http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html

------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 08:32:10 2005



From: donc-at-asmicro.com (by way of MicroscopyListserver)
Date: Sat, 9 Apr 2005 08:48:15 -0500
Subject: [Microscopy] viaWWW: AFM - want used Dimension scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (donc-at-asmicro.com) from on Friday, April 8, 2005 at 14:30:20
---------------------------------------------------------------------------

Email: donc-at-asmicro.com
Name: Don Chernoff

Organization: Advanced Surface Microscopy Inc

Title-Subject: [Microscopy] [Filtered] MListserver: AFM - want used Dimension scanner

Question: Where can I buy a used Dimension AFM scanner?


If you are not familiar with it, this is a component of Dimension-series (3000, 3100, 5000, etc...) large sample AFMs sold under the NanoScope brand by Veeco Metrology/Digital Instruments.
Identifying photos are shown at:
http://www.asmicro.com/Equipment/Identifying_NanoScope.htm

Please reply directly to me:
donc-at-asmicro.com

regards,
Don Chernoff
{business activities: analytical services in AFM, AFM probes, calibration and test specimens, calibration and measurement software, used NanoScope equipment.}
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd. Suite 120 Voice: 317-895-5630
INDIANAPOLIS IN 46220 Toll free: 800-374-8557 (in USA)
web: http://www.asmicro.com Fax: 317-895-5652


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 17:54:18 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Sat, 09 Apr 2005 16:09:17 -0700
Subject: [Microscopy] Re: Scanning 3 1/4" X 4" Negatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Epson 4870 does an excellent job for all negs 4 x 5 and smaller.
One can also use their 3200 Scanner which is a bit cheaper. It doesn't
scan as many negs at once as the 4870, but works fine.

Some of the lower end Microtech scanners also do a decent job.

Judy


Judy Murphy, PhD
Microscopy Training, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net


Garry Burgess wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 17:56:48 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Sat, 09 Apr 2005 16:12:03 -0700
Subject: [Microscopy] Re: "Dualbeam" "Crossbeam" need a better name

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How about Two Beam (not very creative I guess, but perhaps workable)

Judy


Judy Murphy, PhD
Microscopy Training, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net


Richard Edelmann wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 9 18:12:44 2005



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Sat, 9 Apr 2005 13:28:29 -1000 (HST)
Subject: [Microscopy] Tips for M&M05 in Hawaii

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello, All-

I've lived in Hawaii for just short of 37 years and, like the native New
Yorker who has never been to the Statue of Liberty, I'm not a good tour
guide for HOnolulu in its current incarnation. But I'm getting daily
emails from microscopists about Microscopy & Microanalysis 2005 here in
Honolulu, asking about hotels and transportation, so here are some
observations that might help you.

Waikiki is about a mile end to end, and the Honolulu Convention Center is
at the far western end, actually a bit beyond Waikiki proper, and not next
to the bulk of the hotels. Waikiki is flat and an easy walk. The three
meeting hotels are about 20 and 25 minutes' walk from the Convention
Center, and both the Sheraton Waikiki and the Hyatt Regency Waikiki will
have shuttle buses to the Convention Center for meeting attendees. Meeting
rates for both hotels are very good compared to their published
prices. Both larger hotels have lots of amenities and seasonal children's
programs (better check first), and I think that people who choose the
Sheraton Princess Kaiulani have privileges at the other Sheratons as well.

For those who choose not to stay in the above hotels, you might find some
budget hotels, especially those away from the beach, such as along the Ala
Wai Canal. Be aware that whole blocks at the western end of Waikiki are
about to be torn down for a major new development. If you are nostalgic
about rooms and eateries along part of Lewers (the makai, or ocean end),
Beachwalk, Saratoga and Kalia, they will probably be a big hole in the
ground in August! This will also put pressure on hotel rooms, so please
book soon if you have not already. There are very few hotels outside of
Waikiki, except for a few high-end resorts. If you are looking for cheap
accommodations, be aware that you will be competing with lots of others
who are relatively poor, such as the nearly homeless and drug-addicted.
Waikiki is currently pretty safe, but you will also find those elements
there. It's a big city and resort area with the usual problems; please
exercise good judgment!

Traffic is really heavy in Waikiki and around the Convention Center, and
parking is at a premium. I personally recommend that you do not have a car
for the meeting portion of your stay, and then rent a car for sightseeing
before or after the meeting. To get to the Convention Center by city bus
(TheBus) from Waikiki, take the #2 or #13 bus (westbound) on Kuhio
Ave. Get off on the corner of Kalakaua Ave. and Kapiolani Blvd. in front
of Hard Rock Cafe, then cross the street (scary intersection!) to the
Convention Center. TheBus (http://www.thebus.org, $2 per trip) is a pretty
good way to get around island-wide. If you have family accompanying you,
they will find lots to do within Waikiki or on tours, easily booked in the
lobby of most hotels, and they may be able to do without a car until you
can join them.

There is a recently published book that is pretty thorough, Oahu
Revealed; the Ultimate Guide to Honolulu, Waikiki & Beyond by Andrew
Doughty and Harriett Friedman. It has good maps, hotel and food reviews
and tips, and I recommend it. The authors have also covered Maui, Kauai
and the Big Island, and those books are supposed to be excellent.

I think airfares just hopped up, but most people I've talked to have been
able to find some pretty good deals. You might look for fly/room/car deals
and deals that combine inter-island travel as well. Travel agents often
have access to excursion rates. Take heart in knowing that you will be
able to get to Hawaii and back cheaper than I can get to the Mainland and
back! I find Microscopy & Microanalysis worth the expense each year,
anyway. With an excellent program and over 1100 papers, this will be the
biggest M&M ever! And you won't want to miss the usual fabulous trade
show. I hope to see all of you in a few months.

Aloha,
Tina

Microscopy & Microanalysis 2005
Local Arrangements Committee Co-Chair

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From MicroscopyL-request-at-ns.microscopy.com Sun Apr 10 11:24:13 2005



From: somayyeh_kheiri-at-yahoo.com (by way of Ask-A-Microscopist)
Date: Sun, 10 Apr 2005 11:43:25 -0500
Subject: [Microscopy] AskAMicroscopist: counting of pollen grains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, April 10, 2005 at 10:55:17
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh

Organization: kheiri

Education: Graduate College

Location: urmia,Iran

Question: I have been counting the number of pollen grains and ovules to get the
pollen/ovule ratio for verbascum species
and populations.

I pressed the anthers of the flowers and provided a susponsion of the
debris of anthers in distilled water to count the pollen
grains in a 5 microliter volume.

some of the samples in the susponsion were counted
one day after they were provided so the result showed
differences from the first sample which was counted immidiately after
providing the susponsion.
I myself thought keeping the material in water for oneday
caused the grains to be attached together and the amount of the grains
that are counted are reduced.

can anybody help me solve the problem?

is this reasonable or there maybe errors in the method of counting?

I appreciate any kind reply.

Somayyeh


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 08:04:16 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Mon, 11 Apr 2005 09:22:27 -0400
Subject: [Microscopy] Re: Tips for M&M05 in Hawaii

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tina,
Thank you for the advice. See you in August.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 08:21:57 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Mon, 11 Apr 2005 08:41:28 -0500
Subject: [Microscopy] Administrivia: March Archives On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues....


The March Archives are now available at:

http://www.microscopy.com/MicroscopyListserver


Nestor
Your FriendlyNeighborhood SysOp


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 08:56:50 2005



From: Lou Ross :      RossLM-at-missouri.edu
Date: Mon, 11 Apr 2005 09:15:29 -0500
Subject: [Microscopy] 3rd annual Short Course on Computer-Assisted Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Electron Microscopy Core Facility at the University of
Missouri-Columbia is hosting the 3rd annual Short Course and Workshop
on Computer-Assisted Image Analysis and Measurement taught by Dr.
John C. Russ on May 25-27, 2005. This popular course is intended to
familiarize users of image analysis equipment with the fundamental
principles and methods available to obtain meaningful results, and to
educate laboratory supervisors or research professionals seeking to
learn how to use such methods in their applications. The techniques
are applicable to fields ranging from materials, geological and
biological/medical research to food technology and manufacturing
quality control.

The course relies heavily on tightly coupled lectures and hands-on
experience with the various techniques. The laboratory includes a
wide variety of image analysis methods designed to cover the range of
approaches and tools, and a detailed set of practical instructions to
enable their use with a minimal learning curve. No specific
background is assumed, although users should already be familiar with
microscopy or other imaging technology, and the techniques required
to obtain the images to be measured. Many of the examples used in the
course involve light or electron microscope images, but students are
invited to bring their own most interesting images (TIFF files) for
discussion and analysis.

Image analysis and measurement methods are used in a broad range of
applications and are usually concerned with extracting a few
numerical values, such as the number, size, shape or location of
objects from the image. In other cases, global structural parameters
such as measures of the volume and surface of structures present are
of interest. These measurements may require image processing to
correct defects, feature enhancement, comparison of multiple images,
object recognition, or other steps. Ultimately, the image is reduced
to just the features of interest. Measurements on these individual
features, or on the image as a whole, must then be obtained and
interpreted in a proper stereological context to obtain useful data
about the objects. Statistical interpretation of the data allows
comparisons of different populations, understanding of distribution
plots, and other inferences about the original objects. Structural
modeling and geometric probability can be used to develop models for
this interpretation.

Dr. Russ is the internationally acclaimed author of innumerable
articles and several books on image analysis techniques and digital
imaging, including the The Image Processing Handbook. He is widely
known for his workshops and short courses and has helped to develop
software packages to make image analysis methods more accessible to
non-specialists.

The registration fee is $1100 and has an enrollment limit of 20.
Registration deadline is April 29. More information can be found at
{http://www.emc.missouri.edu/works.htm} http://www.emc.missouri.edu/works.htm,
or by contacting course coordinator Lou Ross at 573.882.4777 or
rosslm-at-missouri.edu.

Lou Ross
--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 10:34:48 2005



From: John Shields :      jpshield-at-uga.edu
Date: Mon, 11 Apr 2005 11:53:42 -0400
Subject: [Microscopy] SEMS meeting in Pensacola

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Just a reminder...
Greetings Members,

The Southeastern Microscopy Society's 41st Annual Meeting is
fast approaching us. The Meeting Dates are May 18-20, 2005
and will be held at the Hilton Garden Inn, Pensacola Beach,
Florida.

Meeting Information can be found on the SEMS Website at:
http://www.semicroscopy.org/

As a reminder, please note the following deadlines and
Abstract Due Date Extension:*

*1. Deadline for receipt of abstracts (Including Ruska Award
Participants) was April 10, 2005 to Jim Sheetz, (see address
below).
THE DEADLINE FOR ABSTRACT SUBMISSIONS HAS BEEN EXTENDED TO
APRIL 18!!!!

Abstract Submissions to BOTH:
Dr. Jim Sheetz
Dept. Cell Biology
Vocker Hall, Box 302
Univ. of Alabama at Birmingham
1670 Univ. Blvd.
Birmingham, AL 35294
and
Dr. John P. Shields phone:706-542-4080
EM Lab e-mail: jshields-at-cb.uga.edu
151 Barrow HallUniversity of Georgia

Questions or problems with preparation of abstracts - please
contact the Proceedings Editor (John
Shields: phone: 706-542-4080 or e-mail: jshields-at-cb.uga.edu.

2. Hotel Reservation Deadline: April 17, 2005

Please note that reservations made after April 17 will be
subject to rates based on availability at the time the
reservations are made. After April 17, please continue to
ask for "Group Reservations" when calling the Hilton and use
Group/Convention code SMS so that SEMS will be credited.

Property Location and Reservation Information
Hilton Garden Inn
12 Via de Luna Drive
Pensacola Beach FL 32561
1-866-916-2999 Ask for "Group Reservations"
3. Conference Registration Fee(s) Deadline: Early
Registration APRIL 25
To register, please mail form to:
Ms. Karen L. Kelley Phone: (352)-392-
1184
University of Florida Fax: (352)-846-
0251
P.O. Box 118525
Gainesville, Florida, 32611
John P. Shields
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602

706-542-4080


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 13:13:13 2005



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 9 Apr 2005 12:07:12 +0100
Subject: [Microscopy] Re: RE: HR images-Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All

[This note is with the blessing of Randy Tindall]

I think we have a bit of a problem with Randy's explanation of secondary
electron signals?

} My understanding is that there are several factors involved in selecting
} an accelerating voltage (KV). At higher KVs, chromatic aberration is
} reduced, resulting in greater resolution, i.e., the ability to separate
} two points visually. However, higher KVs also result in the generation
} of secondary electrons from a larger surface area, due to primary beam
} electron scattering over a larger lateral distance, as well as from
} deeper within the specimen

I have to dispute the second sentence. Secondary electrons have an energy
of less than 50eV be they produced from a 500v beam or a 1MeV beam. Thus
their mean free path within the specimen is reduced to less than 15nm!
David Joy explains that 80% of the SE signal comes from the top 5nm, around
15% from the next 5nm and less than 5% from the final 5nm.

} This theoretically has two effects. One is that the larger area of the
} surface emitting SE's gives a larger "effective spot size", or area from
} which signal is generated, and this decreases resolution. The other is
} that the SEs escaping from deeper within the specimen are detected along
} with the SEs generated at or just below the surface, and this part of
} the signal can obscure surface detail by lowering contrast. This effect
} can easily be seen by taking a biological specimen or other soft sample
} and simply comparing a 1.0 KV image to a 30 KV image: the higher KV
} image looks smoother and less detailed.

Higher kV actually produce a probe nearer to the theoretical probe size than
do lower kV where aberrations and external fields play their part in
disturbing the beam. I would agree that the higher the kV the higher the
emission of backscattered electrons and these are dependant upon incident
beam energy. In light element materials like biological specimens a 30kV
beam may bring backscattered information from as much as 3um below the
surface and it is this information that may blur the image and spoil
resolution. BSE you see come from the full width of the reaction volume and
are therefore at a much lower spatial resolution. It is BSE not SE that are
spoiling images. If you increase the kV and the specimen becomes more
interesting it is the sub surface detail (BSE) that is bringing the interest
as the BSE overwhelm the SE. Lowering the kV the number of BSE are reduced
and the SE start to dominate. In a material science specimens the sub
surface detail may be of more interest than the surface; cracks, porosity
and different phases with different density etc etc.

} This effect is complicated by the effect of sample density, in that a
} denser sample will have a smaller "effective spot size" than a
} less-dense sample at the same KV. This means that for many samples you
} can increase resolution by increasing KV if the sample is dense enough
} to restrict the electron scattering to acceptable levels for the
} magnification you are working at. In addition, higher KVs generally
} allow such adjustments as using smaller apertures to decrease spherical
} aberration effects and increase depth of field, and higher lens
} currents to demagnify the probe size and increase resolution that way.
} Generally speaking, adjusting the SEM for high resolution means
} decreasing the signal to the detectors, so if you have more signal to
} begin with, you can afford to lose more signal before noise overwhelms
} your image.

The biggest gain in performance is to optimise the kV for the task in hand.
Balancing resolution with information is more important than out and out
resolution even when working a FEG system at 350,000X! As a general
statement biological specimens will be best between 2 and 10kV, other light
elements (Al, Si) 5 to 10kV, nickel, iron and copper alloys 7 to 15kV. The
performance of a FEG SEM like any other often depends upon emission current
rather than kV. Optimise the kV for the specimen then tune the emission
current to optimise performance and signal when apertures and spot sizes are
brought into play. It may interest you to know that working with an Intel
laboratory we found on a Hitachi S4500 we only needed spot size 8 to achieve
the highest resolution working at 4mm 15kV. Smaller spot sizes in a FEG SEM
usually do not have the same advantage as in a tungsten hairpin instrument.
Short working distances, good alignment of the gun and top notch alignment
for the aperture (up to the magnification you intend to record the image)
are far more important than putting effort into "reducing aberrations" by
other means.

} I agree with you that for many samples, especially biological ones,
} lower KVs are better at retaining surface detail in the image. We
} routinely run our FESEM at 5.0 KV, and often at 1.0 KV. But not always.
} Separating out the relative effects of larger "effective spot sizes"
} (decreased resolution), escape of SE's from deeper within the specimen
} (lower surface contrast), and decreased aberrations (increased
} resolution) at high KV's is not straightforward. And let's not even get
} into the effects of coatings on these factors, since you may need
} heavier coatings to counter increased charging effects, as you
} mentioned! As I said, it's probably best to approach each sample as
} unique and experiment.

Please forget about SE from greater depths and concentrate on balancing the
information you require. Very short WD ( {5) on a 4500/4700 restricts the
imaging data to SE which can be very boring and this restriction vastly
increases the possibility of charge. Move away from the lens a little (~7
to 9mm) and you bring in some of the BSE information that has been converted
to secondaries, these will provide added contrast, some image depth and most
of all less charge.

} By copy of this to the listserv I am asking to be set straight on any
} errors in the above sermon. I always learn a lot from these discussions
} and have had faulty assumptions corrected on several occasions.

I have a high regard for Randy hence this reply is not going to the
listserver unless he requests it? I do not remember if I gave Randy a copy
of the "Working With a SEM CD" where you will find a full explanation of
signals. If I have time I will put something on my web site in Hints and
Tips - Monday.

Regards to you all

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
www.emcourses.com tel +44 1280 816512 fax +44 1280 814007



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 15:02:07 2005



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 11 Apr 2005 10:20:58 -1000 (HST)
Subject: [Microscopy] Top 10 Reasons to Attend M&M 2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Top 10 Reasons your Boss Should Send You to M&M 2005 in Honolulu

1. You'll be networking with colleagues from the 7 participating
microscopy societies, making great international contacts and developing
new scientific collaborations

2. You'll see the latest and greatest in instrumentation and techniques
at the M&M exhibit

3. Pre-meeting congress and short courses followed by a compelling
scientific program of over 1100 papers from 40 different countries will
prevent you from getting a sunburn

4. Vendor and sponsor tutorials in new and emerging technologies will
keep you up-to-date

5. Free popcorn and beer in the exhibit hall/poster sessions will keep
you off the beach

6. You have a chance to win a diamond knife and other goodies for your
lab

7. You can interact with vendors so that they know what you need in a
product

8. Face-to-face problem solving with colleagues and experts in the field

9. With the time zone difference you can get most of a day's work done by
internet before even going to the meeting

10. You promise to teach everyone the hula when you return



Keep up with the news on the meeting website
http://mm2005/microscopy.org




****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 15:53:09 2005



From: Scott Griffith :      skod-at-ises-llc.com
Date: Mon, 11 Apr 2005 15:12:08 -0600
Subject: [Microscopy] Searching for Leitz Ergolux AMC documentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sirs:

I'm a new list member who would like to contact anyone on the list who
might have access to any documentation for an early 90's Leitz Ergolux AMC
optical microscrope, particularly with respect to the optional laser
autofocus hardware and stage controller. I recently acquired one in quite
good condition at an auction, and I am slowly bringing it up to replace my
venerable old Orthoplan with its time-consuming manual stage and focus.
Having this machine up and running will be a tremendous boon to my
business.

The microscope is part number 020-501.015, the laser autofocus module on
the illuminator path is 036-094.102, dated January 1991. The stage is
labelled Wild Leitz 026-407.110, but looks identical to a number of
Marzhauser stages I've dealt with in the past (rectangular motor cases
with no manual knobs). The joystick/controller pad is one of the older
black Leitz units with a 2-line LCD readout and a DB25 interface to the
electronics rack, numbered 301-336.029. And finally, the electronics rack
for the unit that contains the autofocus controller, stage motor drivers,
IEE 488 interface, video hardware, and other ancillary gear is numbered
301-341.150.

Regrettably, Leitz USA has turned out to be essentially no help on this,
and the instrument appears to be more or less completely unsupported.
Therefore, if anyone has some of this ancient documentation gathering dust
on the shelves in the back room (particularly the command set for stage
positioning, and any schematics or the like), please contact me offlist at
the email address below to discuss them. I'd ask that we take this
discussion offlist to preserve listserver bandwidth- because wrenching on
this poor old beast is probably not of general interest!

Thanks very much in advance for any help any of you might be able to
render. I'd be eternally grateful for any feedback on this query.

Yours truly,

Scott Griffith, ISES-LLC
9745 Steeplechase Drive
Franktown, CO 80116
303-660-2541 voice
303-660-2542 fax
skod-at-ises-llc.com


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 11 18:28:01 2005



From: Ben Moshiri :      Ben.Moshiri-at-ametek.com
Date: Mon, 11 Apr 2005 19:47:00 -0400
Subject: [Microscopy] Job Opening - Crystallography (EBSD) Product Manager

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Product Manager, Crystallography Products
EDAX, Mahwah, NJ, USA

Description:
Management of Crystallography (Electron Backscatter Diffraction) products,
including definition of future products, communicating customer/market
requirements to the rest of the organization, executing marketing programs
and providing sales support.

Responsibilities:
Develop product line concepts and position the product line in the
marketplace. Analyze the marketplace to determine growth and technology
trends.
Develop short, medium and long term plans and forecasts for the product
line.
Work closely with other Product Managers to ensure continuity and
coherence in the marketing, design and support of integrated products.

Qualifications and Requirements:
BS or MS in Science/Engineering.
MBA or equivalent highly desirable.
5 years of relevant work experience in a marketing or sales environment.
Previous Product Management experience highly desirable.
Technically knowledgeable about elemental analysis and electron
microscopy.
Excellent communication/interpersonal skills and project management
abilities.

The Company:
EDAX is a leader in EDS, EBSD and Micro-XRF systems, serving customers
across the globe. EDAX is a unit of AMETEK (NYSE: AME), a global
manufacturer of electronic instruments with annual sales of over $1.2
billion.

An excellent compensation package is offered, together with a relocation
package.

Contact Information:
Roberta Burns, HR Manager
Roberta.Burns-at-ametek.com
+1 201 529 4880
www.edax.com



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 10:52:11 2005



From: Mark Robertson-Tessi :      mtessi20-at-yahoo.com
Date: Tue, 12 Apr 2005 09:11:02 -0700 (PDT)
Subject: [Microscopy] Re: HR images-Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

--- Steve Chapman {protrain-at-emcourses.com} wrote:

} Very short WD ( {5) on a 4500/4700 restricts the
} imaging data to SE which can be very boring and this restriction vastly
} increases the possibility of charge. Move away from the lens a little (~7
} to 9mm) and you bring in some of the BSE information that has been converted
} to secondaries, these will provide added contrast, some image depth and most
} of all less charge.

Steve,

I do not see why changing the working distance will reduce charging, all other
things being equal. Whether or not signal is reaches a detector does not
affect charging, I thought. Please explain.

Cheers,
Mark Robertson-Tessi





__________________________________
Do you Yahoo!?
Yahoo! Small Business - Try our new resources site!
http://smallbusiness.yahoo.com/resources/


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 13:50:31 2005



From: Chaoying Ni :      cni-at-UDel.Edu
Date: Tue, 12 Apr 2005 15:08:46 -0400 (EDT)
Subject: [Microscopy] Re: Re: HR images-Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mark,

WD or the geometry between a detector and sample surface does affect the
S/N, especially the SE flux to the detector, hence the contrast of an
image. Charging electrons are primarily those having energies ~10keV or
less (SE counts vs. keV curve). By increasing WD, some of those electrons
may not survive the distance, so less charging is visible. To minimize
charging effects some vendors have designs to filter/block electrons
within a keV window from getting into detectors.

In a sense, you are right, SE electrons of varied energy are produced
anyway with little dependence on WD. But as long as "the charging
electrons" dont get into the detector and dont interfere with the incident
beam, they are not visible.

****************************************
Chaoying Ni, PhD
The W.M. Keck Electron Microscope Facility
Facility location: 022 Spencer Laboratory
Mailing address:
201 duPont Hall
Dept of Matls Sci & Eng
University of Delaware
Newark, DE 19716
(302) 831-2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************


On Tue, 12 Apr 2005, Mark Robertson-Tessi wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} --- Steve Chapman {protrain-at-emcourses.com} wrote:
}
} } Very short WD ( {5) on a 4500/4700 restricts the
} } imaging data to SE which can be very boring and this restriction vastly
} } increases the possibility of charge. Move away from the lens a little (~7
} } to 9mm) and you bring in some of the BSE information that has been converted
} } to secondaries, these will provide added contrast, some image depth and most
} } of all less charge.
}
} Steve,
}
} I do not see why changing the working distance will reduce charging, all other
} things being equal. Whether or not signal is reaches a detector does not
} affect charging, I thought. Please explain.
}
} Cheers,
} Mark Robertson-Tessi
}
}
}
}
}
} __________________________________
} Do you Yahoo!?
} Yahoo! Small Business - Try our new resources site!
} http://smallbusiness.yahoo.com/resources/
}
}


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 15:03:11 2005



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Tue, 12 Apr 2005 21:17:34 +0100
Subject: [Microscopy] Re: Use of trade names

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Perhaps I didn't make myself clear.

I am not objecting to trademarks per se. If somebody has a
distinguishing trademark ("Tecnai" or "Gemini", are obvious examples
in the field of electron microscopy), then I'm all in favour of
providing legal protection to that distiguishing mark.

What I am suggesting is that to trademark simply descriptive terms is
a nonsense. Trademarking, in my personal opinion, of "Dualbeam" or
"Crossbeam" is equivalent to trademarking "Mass Spectrometer" or
"Yellow". It achieves little for the companies concerned, other than
giving companies lawyers work and suggests that the marketing is
being run by some rather unimaginative people. It certainly fails to
generate a distintive brand identity.
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 15:35:13 2005



From: Becky Holdford :      r-holdford-at-ti.com
Date: Tue, 12 Apr 2005 15:53:25 -0500
Subject: [Microscopy] Re: Re: Trade names: was: "Dualbeam" "Crossbeam"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard: as a long-time user of these types of tools, I usually refer to
them as "dual-column" FIBs, unless I'm actually referencing a particular
model.

Richard Edelmann wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 15:47:42 2005



From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Tue, 12 Apr 2005 17:05:51 -0400
Subject: [Microscopy] Re: RE: HR images- Hitachi S-4700 FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm not sure if this is pertinent but check your file parameters when saving
images. These problems do not occur on my system if I save images in TIFF
or JPEG formats. I've only observed this when saving them as Bitmap. I
personally recommend using the TIFF format.

If you are using the Hitachi data manager you select the format upon saving
the image.

If you are using PCI Quartz you need to change this in the PCI.INI file
located on the local PC. Open the PCI.INI file in notepad and make an edit
to the "ImageFileType=" line as shown below:


[Files]
ImageFileType=.TIF
ImageDirectory=
ImportDirectory=C:\aaaacalibration
PhotoCDPage=0

Then save the file. When PCI Quartz is launched it will save the images as
TIF files. I hope this helps?

John A. Robson
Boehringer Ingelheim Pharmaceuticals, Inc.
PO Box 368
900 Ridgebury Rd
Ridgefield, CT 06877

Phone (203)798-5640
Fax (203)798-5698
e-mail jrobson-at-RDG.boehringer-ingelheim.com



-----Original Message-----
} From: bbandli [mailto:bbandli-at-mvainc.com]
Sent: Thursday, April 07, 2005 2:45 PM
To: microscopy-at-microscopy.com

Our JEOL6500-F (JEOL PCSEM software) has the same indexed color
"feature" and converting it to 8-bit greyscale has the same effect of
cleaning up the noise in the image. I also have no idea why.

Bryan Bandli

Tindall, Randy D. wrote:

} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 21:34:41 2005



From: Taylor, Samantha :      Samantha.Taylor-at-alcoa.com.au
Date: Wed, 13 Apr 2005 10:52:40 +0800
Subject: [Microscopy] FEI Quanta 400 FEG

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All

I am looking for information on the FEI Quanta 400 FEG. If you have one
of these instruments - can you please let me know what you think.

Specifically, I am interested in performance, ease of operation, wet
imaging, maintenance issues and support.


Thanks in anticipation.


Samantha Taylor
TDG Experimental Officer
Alcoa World Alumina
XRD, Microscopy and Thermal Analysis
* Phone:(08) 9410 3588
* Fax: (08) 9410 3167
* Email: Samantha.Taylor-at-Alcoa.com.au




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 12 23:22:35 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 12 Apr 2005 21:40:50 -0700
Subject: [Microscopy] Re: Indexed Color RE: HR images- Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

when the SEM makers work up graphics interfaces, I suspect
that they make simple color presentations. This is the
basis of indexed color. Instead of true color (RBG), they
use a palette of colors which are indexed by the pixel value.
This is typically 4 bits (8 colors) or 8 bits (16 colors).
This reduces the overhead for defining and dealing with
colors. Since the SEM image itself is greyscale, the annotation
and legends are typically of some set of colors. These are
colors out of the palette.

When the image is saved, the result is all grey scale, usually.
hence, the better observable quality. But for those programs
that do not store pure grey scale, Photoshop will allow Mode
change to greyscale. Then, other programs can process the
image file no problem.

JPEG is by definition a true color system--RGB. However, some
apps don't adhere to this. The basic theme is to figure out
what mode your image files are and convert them to greyscale.
Or, convert them to RGB if you want to pseudo color them.

gary g.



At 02:05 PM 4/12/2005, you wrote:


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From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 03:45:04 2005



From: yezer-at-cc.hut.fi
Date: Wed, 13 Apr 2005 12:03:01 +0300 (EEST)
Subject: [Microscopy] HR images with Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

Thank you for the suggestions and comments for achieving high resolution
images. We will review them and reply with feed back. We are looking for a
shareware program for calculating thin films thickness by SEM. If any one know
one and can recommend we will appreciate.

Regards,
Ezer Yossef


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 08:09:31 2005



From: cpotter-at-scripps.edu (by way of MicroscopyListserver)
Date: Wed, 13 Apr 2005 08:28:39 -0500
Subject: [Microscopy] viaWWW: A Practical Course in Molecular Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cpotter-at-scripps.edu) from http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April 12, 2005 at 14:26:52
---------------------------------------------------------------------------

Email: cpotter-at-scripps.edu
Name: Clint Potter

Title-Subject: [Microscopy] [Filtered] A Practical Course in Molecular Microscopy

Question: The National Resource for Automated Molecular Microscopy
Sponsored by the National Center for Research Resources

Announce

A Practical Course in Molecular Microscopy
November 2-10, 2005
National Resource for Automated Molecular Microscopy
The Scripps Research Institute (TSRI), La Jolla, California.

We invite applications to participate in a Practical Course in Molecular Microscopy. This biennial course is part of the outreach and training activities of the NCRR National Resource for Automated Molecular Microscopy (NRAMM), at the Center for Integrative Molecular Biosciences (CIMBio) at The Scripps Research Institute (TSRI). The course is aimed at approximately 40 participants who want a thorough grounding in all aspects of molecular structural determination by cryo-electron microscopy (cryoEM).

The course will include an extensive exposure to all of the practical aspects of cryoEM as well as a solid grounding in the theory. The basic format will be theoretical lectures in the morning followed by hands-on lab sessions and demonstrations in the afternoon. The lab sessions will include instruction in specimen preparation, use of the electron microscope for imaging of cryo specimens, evaluation of the images, and a thorough treatment of the image analysis techniques required to reconstruct three dimensional maps. In addition there will be a number of afternoon lectures introducing participants to research topics in the area of macromolecular structure. Evenings will be reserved for presentations by participants and for helping participants with their own projects.

The instructors for the course include: Francisco Asturias (TSRI), Tim Baker (UCSD), Charlie Brooks (TSRI), Nicolas Boisset (Paris), Anchi Cheng (TSRI), Wah Chiu (Baylor), David de Rosier (Brandeis), Joachim Frank (Albany), Yoshi Fujiyoshi (Kyoto), Bob Glaeser (Berkeley), Niko Grigorieff (Brandeis), Dorit Hanein (Burnham), Elizabeth Kubalek (TSRI), Steven Ludtke (Baylor), Ron Milligan (TSRI), Pawel Penczek (Houston), Clint Potter (TSRI), Tanvir Shaikh (Albany), Vinzenz Unger (Yale), Nigel Unwin (MRC), Neils Volkmann (Burnham), Thomas Walz (Harvard) and Mark Yeager (TSRI).

Applications forms may be found online at the workshop web page:
http://nramm.scripps.edu/seminars/2005/cryoem/
Applicants are asked to provide a CV, publication list, and a short description of their current work and future plans. In addition participants will be asked to state a preference for the particular area of image analysis (single particles, viruses, helices, 2D crystals) on which they wish to focus. This will help balance the participation in the lab sessions.

A registration fee of $450 will be charged to participants from non-profit institutions. Meals will be provided as part of the course registration fee but participants will be expected to cover their own travel and lodging expenses. Cost of housing is approximately $60/night for a shared room. The course organizers will help coordinate room sharing arrangements.

The application deadline is 1 July and a final selection of participants will be made by 1 August.

Please see all other information pertaining to the course at the web site:
http://nramm.scripps.edu/seminars/2005/cryoem/

For other inquiries send email to: amiadmin-at-scripps.edu

Organizers
Bridget Carragher, Clinton S. Potter and Ronald A Milligan
National Resource for Automated Molecular Microscopy,
The Scripps Research Institute


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From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 08:08:57 2005



From: somayyeh_kheiri-at-yahoo.com (by way of MicroscopyListserver)
Date: Wed, 13 Apr 2005 08:28:06 -0500
Subject: [Microscopy] viaWWW: thanks Dr Warren E Straszheim for coments about pollen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://www.microscopy.com/MLFormMail.html on Tuesday, April 12, 2005 at 13:52:20
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh

Organization: urmia university

Title-Subject: [Microscopy] [Filtered] thanks Dr Warren E Straszheim for coments about pollen grian counting

Question: Hello Dear Dr Warren

I appreciate your helpful reply about the reason of reuction
in pollen grain counting.
you are right, I have mentioned the big size of pollen grains being kept in water after oneday.
sincerely
Somayyeh

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 08:08:27 2005



From: yagerra-at-aol.com (by way of MicroscopyListserver)
Date: Wed, 13 Apr 2005 08:27:35 -0500
Subject: [Microscopy] viaWWW: good starting microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (yagerra-at-aol.com) from http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April 12, 2005 at 12:52:49
---------------------------------------------------------------------------

Email: yagerra-at-aol.com
Name: Robert A. Yager

Organization: Yager Laboratories

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I want to expand my consulting to optical microscopy analysis of defects in organic coatings. Can anyone recommend a good starting microscope (low $$).



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 09:27:29 2005



From: Ciaburri, Diane A :      Diane.Ciaburri-at-gd-ais.com
Date: Wed, 13 Apr 2005 10:43:40 -0400
Subject: [Microscopy] Job Opening - Failure Analyst

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

We have an opening for a failure analyst. Please direct all inquiries
to me.

Diane Ciaburri
General Dynamics Advanced Information Services
Pittsfield MA
(413) 494-3430




Posted Title: Senior Engineer - Electrical (Failure Analysis)

Job Location: Massachusetts - Pittsfield

Job Requirements:

The candidate should have a minimum of 3-5 years experience in the
evaluation and failure analysis of materials relating to both Electrical
and Mechanical components. Knowledge of Materials Science and Analysis
Lab experience is required. Experience in one or more of the following
is preferred: Scanning Electron Microscopy, Infrared & Optical Imaging,
metallurgy, thermal analysis, chemistry, coating techniques, X-ray
analysis. The candidate must be proficient with Microsoft Windows and
be capable of utilizing software tools relative to image processing
(Image Pro, Adobe, etc.) The candidate must also be proficient at
developing and presenting both oral and written test reports and be an
effective communicator who can work "real-time" with designers to solve
complex issues. The Senior Engineer - Electrical will be an active
participant in implementing and improving the GDAIS Common Process
relative to both failure analysis and general processes.

BS (EE, ChemE, Physics, Chemistry, Materials Engineering) or related
area with a minimum of 3 to 5 years experience in material analysis or
related areas (Required)
MS (Desired)

Other Job Requirements:
Strong communication and team skills.
Dedication to ethics, diversity and safety.
Experience in the following: Scanning Electron Microscopy, Infrared &
Optical Imaging, metallurgy, thermal analysis, chemistry, coating
techniques, X-ray analysis. (Preferred)
Experience with Microsoft Windows (Required)
Knowledge of Image processing tools and techniques (Preferred)
Proficient at identifying and developing process and quality
improvements.
Dedication to meeting assigned budgets and schedules.
Minimum travel required.
Relocation negotiable


Specific Responsibilities:

The Senior Engineer - Electrical (Failure Analysis) is responsible for
designing and conducting tests for physical, electrical, and mechanical
attributes to determine production/process anomalies, causes of failure,
and recommend corrective action.

Responsibilities include:

Perform material evaluation and failure analysis of samples provided by
Engineering and Operations.
Work with designers to understand underlying issues leading to a root
cause of the failure.
Generate reports and oral presentations documenting the tests performed,
data taken and conclusions/recommendations.
Improve current material evaluation and failure analysis processes and
techniques.




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 09:30:04 2005



From: Scott Griffith :      skod-at-ises-llc.com
Date: Wed, 13 Apr 2005 08:49:03 -0600
Subject: [Microscopy] Re: Searching for Leitz Ergolux AMC documentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, here's a report of customer service that exceeded my expectations by
a good fraction. Within 12 hours of my plea for Ergolux documentation, I
got a response from not only Ms. Ziegler below, but also from the Leica
Microsystems Denver area dealer (Gary Hanson), who is just a few miles
away. So, it would appear that I have simply been talking to the wrong
people- showing once again the value of widely-distributed lists like this.

I now have some documentation on the way from Mr. Hanson, which should
help somewhat with restoring the stand. I still need documentation on the
stage and electronics rack (particularly cable pinouts), which will
hopefully come from Germany or from some helpful list member- so don't
stop dusting off any old manuals, please! But the instrument is now a lot
closer to being productive again than it was.

My thanks to Ms. Ziegler to making the extra effort. Unsupported hardware
or not, there are still folks out there who want to take care of their
customers.

On Tue, 12 Apr 2005 07:50:10 -0500,
{Gretchen.Ziegler-at-leica-microsystems.com} wrote:

} I will send out a search with our dealers, and see if anyone in Germany
} has anything for you.
}
} Gretchen
}
} Gretchen Ziegler, Sales Manager, Educational Division
} Leica Microsystems, Inc.
} P.O. Box 151 - Ocean Grove, NJ 07756
} Phone: 732-897-9506 - Fax: 847-236-3013
} Voicemail: 1-800-248-0665 ext. 5131

-skod


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 13 09:34:38 2005



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Wed, 13 Apr 2005 15:53:31 +0100 (BST)
Subject: [Microscopy] Relocating a slide position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

The old chestnut about relocating that area of
interest on a slide. I've used my own England Finder,
but have not recently used any other methods on a
non-motorised stage.

Anyone out there with alternative favourite methods?

I want to ensure that I include other possibilities
within a lecture I'm going to give....

Regards,
Jeremy

Send instant messages to your online friends http://uk.messenger.yahoo.com


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 14 09:12:22 2005



From: gazzoman-at-hotmail.com (by way of MicroscopyListserver)
Date: Thu, 14 Apr 2005 07:33:50 -0500
Subject: [Microscopy] viaWWW: PET-100x

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (gazzoman-at-hotmail.com) from http://www.msa.microscopy.com/MLFormMail.html on Wednesday, April 13, 2005 at 14:44:27
---------------------------------------------------------------------------

Email: gazzoman-at-hotmail.com
Name: Daniele Gazzola

Organization: university of bologna

Title-Subject: [Microscopy] [Filtered] PET-100x

Question: I would appreciate if somebody could let me know if exists an immersion oil with which I can use a 100x obj. with a PET coverslip.
I believe that PET index of refraction is about 1.64.

Thanks,
Daniele

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 14 10:05:31 2005



From: ke :      kevans1-at-nycap.rr.com (by way of Ask-A-Microscopist)
Date: Thu, 14 Apr 2005 07:30:15 -0500
Subject: [Microscopy] AskAMicroscopist: Microscope restoration Project

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello

I wonder if you might be able to help me.

My name is Kenneth Evans and I am a retired Chemistry instructor in upstste New York (Albany region). I have recently acquired a microscope which I am intending to restore. I have been unable to find but four or five references to it on the internet and therefore I have been asking for help from people like yourself .

The scope may be called a Quantimet 720 and it may have been part of a metallurgical system produced by a company called IMANCO in or about 1969. I do not know if they produced the scope or just the "system" in which the scope was used. There seems to be little record of them on the net.

Any information you might be able to provide would be greatly appreciated.


Thank you1

kenneth



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 14 11:51:20 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Thu, 14 Apr 2005 10:10:29 -0700 (PDT)
Subject: [Microscopy] Re: AskAMicroscopist: Microscope restoration Project

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kenneth:

I believe the microscope was part of a much larger
unit, similar to the relic I once tried to resurrect
at my previous position. The Quantimet unit was used
for image analyzing. I imagine the microscope can be
used on its own without the image analyzing system.

Are there any other brand names on the microscope? I
find it hard to imagine they made a microscope
dedicated just for this system, versus adapting an
existing make to their unit.

Stu Smalinskas, PE
Metallurgist
SKF
Plymouth, Michigan

--- ke {kevans1-at-nycap.rr.com} wrote:
}
}
}
------------------------------------------------------------------------------
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} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
}
} Hello
}
} I wonder if you might be able to help me.
}
} My name is Kenneth Evans and I am a retired
} Chemistry instructor in upstste New York (Albany
} region). I have recently acquired a microscope
} which I am intending to restore. I have been unable
} to find but four or five references to it on the
} internet and therefore I have been asking for help
} from people like yourself .
}
} The scope may be called a Quantimet 720 and it may
} have been part of a metallurgical system produced by
} a company called IMANCO in or about 1969. I do not
} know if they produced the scope or just the "system"
} in which the scope was used. There seems to be
} little record of them on the net.
}
} Any information you might be able to provide would
} be greatly appreciated.
}
}
} Thank you,
}
} kenneth
}
}
}



__________________________________
Do you Yahoo!?
Make Yahoo! your home page
http://www.yahoo.com/r/hs


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 14 13:37:32 2005



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Thu, 14 Apr 2005 13:29:32 -0400
Subject: [Microscopy] Re: viaWWW: PET-100x

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Cargille labs has a wide range of immersion fluids.
See http://www.cargille.com/opticalintro.shtml



} Email: gazzoman-at-hotmail.com
} Name: Daniele Gazzola
} Organization: university of bologna
} Title-Subject: [Microscopy] [Filtered] PET-100x
}
} Question: I would appreciate if somebody could let me know if exists an
} immersion oil with which I can use a 100x obj. with a PET coverslip.
} I believe that PET index of refraction is about 1.64.
}
} Thanks,
} Daniele

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 07:31:05 2005



From: t.keller-at-uni-jena.de (by way of MicroscopyListserver)
Date: Fri, 15 Apr 2005 07:50:20 -0500
Subject: [Microscopy] viaWWW: Jeol operation at different voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (t.keller-at-uni-jena.de) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, April 15, 2005 at 03:54:57
---------------------------------------------------------------------------

Email: t.keller-at-uni-jena.de
Name: Thomas Keller

Organization: University of Jena

Title-Subject: [Microscopy] [Filtered] Jeol operation at different voltage

Question: Dear all,

I would like to operate our TEM (JEOL 3010) at different voltages (300 kV, and 120 kV / 200 kV). Is that possible without much realigment each time?

Thanks for your help
Thomas Keller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 07:31:18 2005



From: helen.gruber-at-carolinashealthcare.org (by way of MicroscopyListserver)
Date: Fri, 15 Apr 2005 07:50:43 -0500
Subject: [Microscopy] viaWWW: TEM tech position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (helen.gruber-at-carolinashealthcare.org) from http://www.msa.microscopy.com/MLFormMail.html on Friday, April 15, 2005 at 06:11:02
---------------------------------------------------------------------------

Email: helen.gruber-at-carolinashealthcare.org
Name: Helen E. Gruber, Ph.D.

Organization: Carolinas Medicial Center, Charlotte, NC

Title-Subject: [Microscopy] [Filtered] MListserver: TEM tech position available

Question: Transmission Electron Microscopy technician position is available immediately. Clinical/research work. Certification preferred but not essential. Previous TEM experience required; immuno experience is advantageous. "Hard money" hospital funded position (not grant funded). Salary range is for a Research Lab Tech II, $13.10-19.65/hour. 401k and health/dental benefits. Send me your resume, history of your TEM experience, and names and contact info for 3 references.

Carolinas Medical Center is an Academic Medical Center Teaching Hospital and the flagship facility of Carolinas HealthCare System. Carolinas HealthCare is an equal-opportunity, not-for-profit, self-supporting public organization offering a wide variety of health and human services to residents of both North and South Carolina. Carolinas HealthCare System is the largest healthcare system in the Carolinas, and fourth largest public system in the nation

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 07:32:34 2005



From: tony.j.savage-at-gsk.com (by way of MicroscopyListserver)
Date: Fri, 15 Apr 2005 07:51:59 -0500
Subject: [Microscopy] AskAMicroscopist: Microscope restoration Project

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





The Quantimet 720 was an early image analyser which was developed and manufactured in the UK by a Company called Cambridge Instruments. A few years ago, this company was amalgamated into the Leica Group. I suspect that there may be a few people around who could still help you in this restoration project. You could try by approaching their UK address: Leica UK Ltd, Davy Avenue, Knowl Hill, Milton Keynes. MK5 8LB. UK. (tel. 00 44 01908 666663). Good luck
Regards,
Tony
Histopathology Group
Asthma Biology Department.
RIRP CEDD.
GlaxoSmithKline Medicines Research Centre,
Gunnelswood Road,
STEVENAGE,
Hertfordshire.
SG1 2NY
tel. +44 (0)1438 764117
fax. +44 (0)1438 764782
email. Tony.J.Savage-at-gsk.com
mobile +44 07753609835
http://ukdiscovery.gsk.com/histopathology/default.htm


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 07:58:31 2005



From: frank.karl-at-degussa.com
Date: Fri, 15 Apr 2005 08:23:03 -0400
Subject: [Microscopy] Re: Re: viaWWW: PET-100x

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





The bigger question is, is the working distance of the oil emersion lens
long enough to ue a PET cover slip. In many cases a number 1 slip is used
instead of a 1.5 slip. Some folks have been known to put the specimen on
the slip (blood smear or thin section) and not the slide. Working distance
rules in oil emersion. Don't forget to use an oilable condenser, 'cause
resolution is a function of the lowest refractive index in the
condenser/sample/objective system.

Best wishes and good luck!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.



Michael Cammer
{cammer-at-aecom.yu. To: gazzoman-at-hotmail.com (by way of MicroscopyListserver),
edu} microscopy-at-microscopy.com
cc:
04/14/2005 01:29 Subject: [Microscopy] Re: viaWWW: PET-100x
PM







------------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Cargille labs has a wide range of immersion fluids.
See http://www.cargille.com/opticalintro.shtml



} Email: gazzoman-at-hotmail.com
} Name: Daniele Gazzola
} Organization: university of bologna
} Title-Subject: [Microscopy] [Filtered] PET-100x
}
} Question: I would appreciate if somebody could let me know if exists an
} immersion oil with which I can use a 100x obj. with a PET coverslip.
} I believe that PET index of refraction is about 1.64.
}
} Thanks,
} Daniele

____________________________________________________________________________

Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of
Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is
privileged.**







From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 09:32:02 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Fri, 15 Apr 2005 12:11:32 -0230
Subject: [Microscopy] Rolling shutter digital microscope cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

MSA :o)

Can someone explain the how "rolling shutter" digital cameras are different?
I'd especially like to know how this difference affects practical use for
applications in microscopy.

Tia & cheerios ... michael shaffer :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 09:59:55 2005



From: Mike McKay :      mike.mckay-at-pixelink.com
Date: Fri, 15 Apr 2005 11:19:06 -0400
Subject: [Microscopy] Rolling shutter digital microscope cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Michael,

An electronic rolling shutter is similar to a drop-curtain shutter on a film
camera. With a Rolling Shutter, only a few rows of pixels are exposed at
one time. The camera builds a frame by reading out the most exposed row of
pixels (and ceasing exposure of that row), starting exposure of the next
unexposed row down in the Region of Interest (ROI), then repeating the
process on the next most exposed row and continuing until the frame is
complete. After the bottom row of the ROI starts its exposure, the process
"rolls" to the top row of the ROI to begin exposure of the next frame's
pixels.

The exposure down each frame, and from frame-to-frame, remains consistent
due to this continuous read-out.

The row read-out rate is constant, so the longer the exposure setting, the
greater the number of rows being exposed, or integrated, at a given time.
Rows are added to the exposed area one at a time. The more time that a row
spends being integrated, the greater the electrical charge built up in the
row's pixels and the brighter the output pixels will be. As each fully
exposed row is read out, another row is added to the set of rows being
integrated.

Rolling Shutter provides evenly exposed image data with the greatest
possible speed. Because of its speed, a camera in rolling shutter mode is
programmed to free-run, that is to sends frames across the bus as fast as it
can.

Each row of pixels has a slightly different exposure start and end times
from the adjacent rows, so Rolling Shutter can produce a distorted effect
when imaging fast moving subjects, even with very short exposure times. The
distortion is due to the comparatively lengthy process of readout compared
to exposure.

As an example, if using a PixeLINK Pl-A686 camera with 6.6 megapixel sensor,
to readout the entire frame requires approximately 250 milliseconds. While
a short exposure may stop a moving object, the same object can move
appreciably in the quarter second that it takes to readout the frame
resulting in distortion in the direction of motion. The distortion will be
less noticeable on sensors with faster readout times, smaller resolutions
(fewer rows in the ROI) or if strobe/flash illumination is used.

For best results

Rolling shutter should be used with constant illumination and with a static
subject.



Yours,


Michael McKay | Product Manager | PixeLINK
3030 Conroy Road Ottawa, ON K1G 6C2
tel: 613.247.1211 x 152 | fax: 613.247.2001 | http://www.pixelink.com/















-----Original Message-----
} From: michael shaffer [mailto:michael-at-Shaffer.net]
Sent: April 15, 2005 10:42 AM
To: MSA Listserver

MSA :o)

Can someone explain the how "rolling shutter" digital cameras are different?
I'd especially like to know how this difference affects practical use for
applications in microscopy.

Tia & cheerios ... michael shaffer :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 10:49:59 2005



From: MelissaRanieri21-at-aol.com
Date: Fri, 15 Apr 2005 12:09:13 EDT
Subject: [Microscopy] IXRF Open House @ Hitachi Maryland

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

IXRF Systems, Inc. Open House

IXRF would like to invite anyone to an open house being held at Hitachi's
facility in Gaithersburg, MD May 16-18, 2005. IXRF will be demonstrating on a
Hitachi S4800 FE the EDS2004 as well at the new fX SEM Tube that incorperates
XRF analysis in the SEM. We will be scheduling demonstrations on these 3
days from 9 am - 4 pm. For more information on these products please visit our
website at www.ixrfsystems.com or find us on www.microscopy.info.

Please contact Melissa Ranieri -at- melissa-at-ixrfsystems.com or call #
281-286-6485 for further details.

Thank you and have a great weekend everyone!



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 11:10:23 2005



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Fri, 15 Apr 2005 11:29:39 -0500
Subject: [Microscopy] RE: re new anything

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I thought that after the initial emotional impact of the "new anything" post
had worn off a bit, in favor of an alternate opinion, I thought that I'd
contribute my own $0.02 worth here.

So I thought that I'd give my own point by point thoughts on this post,
since it seems to have been provoked by my own post on new ultramicrotomes,
which was primarily prompted by my need for 3 different quotes before a
major purchase can be made in most cases, and in a lot of institutions.

There is indeed brand loyalty that can happen, and sometimes with good
reason, though it is true that new developments may occur, pricing policies
can change, etc. That said, and given that the United States and Canada are
supposedly free countries, I don't see anything particularly wrong or
objectionable about expressing praise or one's brand loyalties. It's sort
of like the Toyota vs. Honda thing. Everyone ultimately realizes that we
are just dealing with opinions, and people also realise that there is a
brand loyalty thing in many cases. And if you are test driving a Toyota
Corolla, the sales person should be aware that you've probably test driven a
Honda Civic. [both of them are good cars by the way!]

That said, I wouldn't myself say too many things about sales, service staff,
since I not only like to give people the benefit of the doubt, but also,
because overall my experience with people in commercial sales has been very
good. So I would agree that it is not desirable to debate someone's
qualifications and style in public. I guess I missed the part of the thread
here that did that.

But when it comes to the point about keeping specific product names off the
listserver, this is a totally different issue. To praise one product
profusely is not necessarily to trash another product. In the post that I
made about scanners for example, I found it extremely helpful to get
specific product names, and model numbers. When I have many people telling
me the same thing, it gives me a lot of confidence in a particular product,
so that I am better able to read between the lines when I read up on the
specs of a given product. It doesn't mean that other products are bad, just
that for a given task, one product might be better suited. And one also has
to remember that price is another variable that keeps everyone from buying
the Mercedes of the lot. I find that generic comments about a product are
almost useless in my opinion, because the main questions that you have are
still left unanswered.

I have participated in many forums on many different sort of topics, and my
experience is that most people don't find a mention of specific products
objectionable, as long as people respect the opinions of those with a
dissenting opinion. Indeed, this can be a way to help other products,
because if they really do have merit, someone who owns it will notice it,
and communicate that to others, especially if such a product is quite a bit
less expensive that the frontrunner.

You can have education as a prime purpose of a listserver where there is
some brand endorsement, and the education won't suffer. People are
generally able to differentiate between the 2 without any adverse affects.

To give a concrete example in another area, I might mention the Nikon vs
Canon debate that one might see on photography forums. Most people can
recognize that it's ultimately a matter of preference, and no doubt those
who own Nikons secretly want into the Canon camp, and vice-versa, but if one
is considering buy a given camera, it is very valuable to be able to hear
comments from an owner of such a camera directly, without having to encode
such talk in generics. And usually the better photographers realize that
the main reason that they have to stick to one manufacturer is primarily
because they have so much invested in lenses, that it's just too expensive
to change sides. One likes to hear about the pros and cons of not only a
given manufacturer, but also a given model and the accessories that are
available for that model. Because if the accessories are not available for
a certain model, that one needs for a specific purpose, then it's better to
know that beforehand than after the purchase. Each manufacturer and model
has it's own strenghts and weaknesses, and it's helpful to know exactly what
these are when one is making a decision about an expensive product. It's
less important when one is buying a pencil.

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 11:24:32 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Fri, 15 Apr 2005 11:43:50 -0500
Subject: [Microscopy] Re: Re: Re: viaWWW: PET-100x

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

One point to remember: the coverslip is an optical element, just like the lenses and prisms in the microscope. The reason that we use #1-1/2 coverslips is the optical path, which is the thickness (0.17mm) times the refractive index (typically about 1.52).
OP = n x t

If the optical path is too thick, you will get spherical aberration. The two best symptoms are that you can't really find a plane of focus and that the image looks milky or "soft".

Since PET has a has RI (n) of 1.64, it makes perfect sense to move down to a #1 or even a #0. My recommendation - get some and try them with YOUR sample.

Hope this is helpful.

Best regards,
Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

We've Moved!
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). For details, call us at 972-943-8011 and ask for Ken Piel.

At 07:23 AM 4/15/2005, frank.karl-at-degussa.com wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 13:03:04 2005



From: Tracy Challman :      tchallman-at-forensic-engrs.com
Date: Fri, 15 Apr 2005 12:38:53 -0700
Subject: [Microscopy] S-2460N Independent Service Contractors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Sorry for any delay but I have been working overseas and could not download
my mail.

Changing the working distance in any SEM varies the type and levels of
signal reaching the Everhart-Thornley detector. Finding a position which
maximises BSE reduces the appearance of charge. At the higher voltage BSE
are not effected by the charge to the same extent as the lower energy SE. An
example of a typical position for the maximum BSE contribution, therefore
the minimum charge position, on Japanese instruments with a conventional
detector position in the specimen chamber is 15mm WD and 45degs of tilt .

The simple way of determining the "minimum charge" position is to form an
image and monitor its wave form. Without changing the gain move the sample
to another position and view the wave form at the same magnification. You
will see a rise or fall depending upon the signal level change. The point
of highest signal will be that of the highest levels of backscatter or
converted backscatter entering the detector; the secondary levels remain
relatively constant.

For more information and ideas on signals take a look at our web site in
Hints and Tips

Regards

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel +44 1280 814774 Direct Line 816512 Fax 814007
www.emcourses.com

----- Original Message -----
} From: Mark Robertson-Tessi {mtessi20-at-yahoo.com}
To: {Microscopy-at-microscopy.com}
Sent: Tuesday, April 12, 2005 5:11 PM

Dear MSA Listserver Colleagues,
 
I have been asked to find alternative service contractors / independent
contractors for our Hitachi S-2460N SEM.
 
I am aware that I may be the one selected as the alternative contractor,
however, I would be very interested to know if others have reliable people
working on their instruments aside from Hitachi's own field engineers.  I
have been VERY pleased with their services.  This issue is cost related,
with hopes of maintaining the quality.
 
Sincerely,
Tracy
 
Tracy Challman
Materials Scientist/Ceramic Engineer
Schaefer Engineering Corporation
23109  55th Ave West
Mountlake Terrace WA 98043-4711
 
Tel.:  425-775-5550    Toll-free:  800-711-0704    Fax: 425-775-0900
 
tchallman-at-forensic-engrs.com       www.forensic-engrs.com
 



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 15 22:44:52 2005



From: amr2w-at-virginia.edu (by way of MicroscopyListserver)
Date: Fri, 15 Apr 2005 23:04:12 -0500
Subject: [Microscopy] viaWWW: 3-D Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amr2w-at-virginia.edu) from http://www.msa.microscopy.com/MLFormMail.html on Friday, April 15, 2005 at 15:22:42
---------------------------------------------------------------------------

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: University of Virginia

Title-Subject: [Microscopy] [Filtered] 3-D Reconstruction

Question: Hello All,
I am interested in doing 3-D reconstruction using the SEM, but not by sectioning. There are a few programs out there that will generate a 3-D model from simply a height map and texture image or from just a stereo pair. Is there a program out there that will use micrographs from multiple angles/rotations for a more true 3-D volume? Thanks for the help!


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 16 00:07:18 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 15 Apr 2005 22:26:40 -0700
Subject: [Microscopy] viaWWW: 3-D Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Try:
http://www.alicona.com/

John Mardinly

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:amr2w-at-virginia.edu]
Sent: Friday, April 15, 2005 9:04 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (amr2w-at-virginia.edu) from
http://www.msa.microscopy.com/MLFormMail.html on Friday, April 15, 2005
at 15:22:42
------------------------------------------------------------------------
---

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: University of Virginia

Title-Subject: [Microscopy] [Filtered] 3-D Reconstruction

Question: Hello All,
I am interested in doing 3-D reconstruction using the SEM, but not by
sectioning. There are a few programs out there that will generate a 3-D
model from simply a height map and texture image or from just a stereo
pair. Is there a program out there that will use micrographs from
multiple angles/rotations for a more true 3-D volume? Thanks for the
help!


------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Sat Apr 16 08:19:15 2005



From: Bill Miller :      microbill-at-mohawk.net
Date: Sat, 16 Apr 2005 09:38:31 -0400
Subject: [Microscopy] Re: viaWWW: 3-D Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Alicona's MeX software will do this..

Bill Miller

At 12:04 AM 4/16/2005, amr2w-at-virginia.edu wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Sun Apr 17 19:49:36 2005



From: James Pawley :      jbpawley-at-wisc.edu
Date: Sun, 17 Apr 2005 20:12:00 -0500
Subject: [Microscopy] Re: RE: HR images-Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} }
} } unique and experiment.
}
} Please forget about SE from greater depths and concentrate on balancing the
} information you require. Very short WD ( {5) on a 4500/4700 restricts the
} imaging data to SE which can be very boring and this restriction vastly
} increases the possibility of charge. Move away from the lens a little (~7
} to 9mm) and you bring in some of the BSE information that has been converted
} to secondaries, these will provide added contrast, some image depth and most
} of all less charge.

Hi all,

I think that there is a way that both are right.

True, SE do not originate from more than about 50 nm inside most specimens.

However, the BSE that do can produce SE "on their way out", as it
where. In fact, on metals, above about 5kV, most SE are produced in
just this way.

With a good modern FEG SEM, I, myself, prefer 1- 1.5 kv for lightly
coated biological specimens.

As far at the "less charge" claim, a lot of SE are produced from BSE
hitting the polepiece, and, I suppose depending on its shape this may
be more or less with longer WD. Although I guess that these might
affect the actual surface charge level (by scattering back to the
sepcimen?), it is important to note that seeing the "anomalous
brightness" effects of charging is merely an indication that the
charge present has messed up the SE collection fields, (usually to
decrease the collection efficiency from nearby details but sometimes
to increase it from the "charged" feature) and I think that we can
assume that the SE collection field in a "below-lens" SEM will
increase (and so be harder to disrupt ) at longer WD.

Cheers,

Jim P.
--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 11-23, 2005, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2005


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 10:17:43 2005



From: Iain Miller :      milleri-at-ohio.edu
Date: Mon, 18 Apr 2005 11:30:03 -0400
Subject: [Microscopy] SEM 3-D reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Photomodeler can be adapted for use with scanning micrographs
http://www.photomodeler.com/

The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (amr2w-at-virginia.edu) from
http://www.msa.microscopy.com/MLFormMail.html on Friday, April 15, 2005 at
15:22:42
---------------------------------------------------------------------------

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: University of Virginia

Title-Subject: [Microscopy] [Filtered] 3-D Reconstruction

Question: Hello All,
I am interested in doing 3-D reconstruction using the SEM, but not by
sectioning. There are a few programs out there that will generate a 3-D
model from simply a height map and texture image or from just a stereo pair.
Is there a program out there that will use micrographs from multiple
angles/rotations for a more true 3-D volume? Thanks for the help!


---------------------------------------------------------------------------

Iain Miller
Department of Biological Sciences
Ohio University
Athens, OH 45701

740-593-2120
milleri-at-ohio.edu


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 10:18:49 2005



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Mon, 18 Apr 2005 10:37:05 -0500
Subject: [Microscopy] TEM-200CX SHP polepiece

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All:
I am looking for someone who has a 200 CX TEM and uses it (or has used
it) with the SHP pole piece or is knowledgeable on the different
configurations possible with the different pole pieces. There are
several pole piece/objective aperture configurations for the 200CX but
we have very little JEOL documentation on the subject. Any real world
experience, expertise or literature that could be shared would be
greatly appreciated. Feel free to contact me offline if you think it
makes sense.
Thanks in advance,
Michael Coviello




From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 18:42:02 2005



From: Ian Hallett :      IHallett-at-hortresearch.co.nz
Date: Tue, 19 Apr 2005 12:06:39 +1200
Subject: [Microscopy] Printing grayscale images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm looking at putting a printer next to our SEM so users can quickly print out a copy of an image without having to go to one of our networked printers elsewhere in the building. I'm interested in good grayscale images - colour is not important. Has anyone any suggestion of printers, I should look at without breaking the budget.

Thanks

Ian

Ian Hallett
HortResearch
Mt Albert Research Centre, Private Bag 92 169
Auckland, New Zealand
Fax +64 9 815 4201
Telephone +64 9 815 4200
EMail ihallett-at-hortresearch.co.nz


______________________________________________________

The contents of this e-mail are privileged and/or confidential to the
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organisation. If you have received this e-mail in error, please notify
the sender and delete all material pertaining to this e-mail.
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From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 19:49:32 2005



From: kushalseth-at-gmail.com (by way of Ask-A-Microscopist)
Date: Mon, 18 Apr 2005 20:11:55 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: HUVEC CELLS ON COLLAGEN AND GELATIN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kushalseth-at-gmail.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, April 18, 2005 at 19:06:57
---------------------------------------------------------------------------

Email: kushalseth-at-gmail.com
Name: kushal Seth

Organization: Texas tech university

Education: Graduate College

Location: Lubbock, TX, USA

Question: I have to grow HUVEC CELLS ON COLLAGEN AND GELATIN NANO FIBRES . AND VIEW THEM UNDER sem .
Itried to grow andf see them but it had BACTERIAS IT SEEMS RATHER THAN HUVECS.
Please suggest viable steps and procedures or any reference papers.
Thanks

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 19:50:04 2005



From: somayyeh_kheiri-at-yahoo.com (by way of MicroscopyListserver)
Date: Mon, 18 Apr 2005 20:12:30 -0500
Subject: [Microscopy] viaWWW: preparing herbarium specimens for sectining in paraffin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (somayyeh_kheiri-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Saturday, April 16, 2005 at 08:53:42
---------------------------------------------------------------------------

Email: somayyeh_kheiri-at-yahoo.com
Name: somayyeh

Organization: urmia university

Title-Subject: [Microscopy] [Filtered] preparing herbarium specimens for sectining in paraffin

Question: Hello Dear all

at first thank you very much for helping me doing my experiments
by giving your useful comments.
I want to provide some section from dried herbarium
leaves. Does anybody know how to prepare the dried ones
for Paraffin embedding?
I just know I should boil the leaves for 2-3 minutes.
but can we use FAA for dried leaves and then usual Etoh series for dehydration like FAA preserved materials or
the herbaruim specimens need other treatments?
I appreciate any kind reply.
Sincerely
Somayyeh


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 19:50:24 2005



From: wong2u23-at-yahoo.com (by way of MicroscopyListserver)
Date: Mon, 18 Apr 2005 20:12:53 -0500
Subject: [Microscopy] viaWWW: Vacuum issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (wong2u23-at-yahoo.com) from http://www.msa.microscopy.com/MLFormMail.html on Sunday, April 17, 2005 at 00:14:39
---------------------------------------------------------------------------

Email: wong2u23-at-yahoo.com
Name: Vic Wong

Title-Subject: [Microscopy] [Filtered] MListserver: Vacuum issues

Question: We have a Hitachi s-4500 FESEM which has until recently worked fine. Recently we have had vacuum trouble, unable to get the specimen chamber to pump down. We have changed the oil in the RP and the diff pump, but still remain at the same pressure. The problem occured when I was exchanging a specimen and when I went to pump down, only the low lamp in the S.C. went on. The SEC has the high lamp on. We are down until this problem is fixed, any suggestions?

Vic

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 19:56:25 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 18 Apr 2005 18:18:43 -0700
Subject: [Microscopy] Re: Printing grayscale images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I use HP LJ 2300dn. There is a 2430 USB or LPR. These
are 1200dpi and have adjustable "dot" characteristics.
These are in the 550-600USD range. You have to check for
optional JetDirect if needing TCP/IP interface. If not on
a network, just use the native parallel.

If you want real cheap, look for a used JP LJ 4M+ or 4+
(about $200USD). These are 600dpi. All of the LJs are
workhorses. The later models are much faster and have
toner saving modes. I use the 4+ and 4MP for many years.
The paper output feeder usually fails after 20,000 sheets.
These are still available for about $80...field replaceable.

If you want to spring for color, the new OKI 5430n is
awesome at $1250USD. Ethernet or parallel or USB and
very fast and high quality color. For pure greyscale,
the b/w printers do a better job, IMO.

gary g.


At 05:06 PM 4/18/2005, you wrote:


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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Apr 18 23:02:07 2005



From: Scott Walck on Comcast :      swalck-at-comcast.net
Date: Tue, 19 Apr 2005 00:21:15 -0400
Subject: [Microscopy] Re: Printing grayscale images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary's solution is similar to what we did at the PPG Glass Technology Center
while I was there. We used a HP LJ2100N which was also 1200 dpi. We set up
our LEO 1350 so that if we printed a 5" wide image on that printer that it
was the same magnification as a stored image. Our standard image output
size was 1024 x 768. However, that did not give us the range in gray levels
of the image. If we were interested in the gray scale of the image, then we
printed an image using the whole page with 1/2" margins. Remember that
there is a tradeoff in grayscale and image pixel resolution for a laser jet
printer because it can only print a dot or not print a dot at the 1200 dpi
resolution. If you are using the 1200 dpi laser printer, the 256 (actually
257) gray levels for a single "pixel" of the image corresponds to an array
of 16 x 16 dots on the printer. At 1200 dpi, this gives 75 16x16 squares
per inch for your image. Your eye has at best (when you were young and
didn't appreciate it) a resolution close to 300 dpi. That means that if a
1024 x 768 digital image is printed at this best eye resolution, it is 3.4"
x 2.56" in size. If I take that same image and print it at 1024 x 768 image
and print it at 10" x 7.5" on a 1200 dpi printer, then I get a factor of
2.9X to expand the gray levels over the image. In other words, my 75 dpi
image now goes to 217 dpi that can be display with all of 256 gray levels.
This assumes a "dumb" laser printer that just uses the 16 x 16 dot array for
printing the grayscale. I don't pretend to know what is going on in the
"smart" laser printers such as the LJ2100N, but the book "Real World
Photoshop" discusses it in much more detail and much more authoritatively
than I can. The net result was that we got pretty good looking images. We
also regularly used the grayscale histogram in the microscope software to
set up our brightness and contrast levels to get as close to using all of
the 256 gray levels as we could.

One other point is that the 2100N was surprisingly fast. I would just wait
until the end of my session and print the images out using Thumbsplus
because I could print the comments and filename along with the image. It
never took very long to print out the images and I would do that as I was
closing down the microscope and removing my sample.

With respect to Gary's suggestion on using the 4+ and 4MP, we had a number
of 600 dpi HP LaserJet printers of this style and some newer styles around
GTC and the quality of the images printed to them depended on the specific
printer. We could get widely varying results printing to the same model
which was not related to the age or usage of the printer. Some simply gave
good results and other didn't. It was a crap shoot. The 1200 dpi printers
always seem to work well for printing images.

I also agree with Gary with respect to color LaserJet printers. The color
laser printers just don't do grayscale images well no matter how you set up
the printer in software.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Monday, April 18, 2005 9:19 PM
To: Ian Hallett
Cc: MSA listserver

I use HP LJ 2300dn. There is a 2430 USB or LPR. These
are 1200dpi and have adjustable "dot" characteristics.
These are in the 550-600USD range. You have to check for
optional JetDirect if needing TCP/IP interface. If not on
a network, just use the native parallel.

If you want real cheap, look for a used JP LJ 4M+ or 4+
(about $200USD). These are 600dpi. All of the LJs are
workhorses. The later models are much faster and have
toner saving modes. I use the 4+ and 4MP for many years.
The paper output feeder usually fails after 20,000 sheets.
These are still available for about $80...field replaceable.

If you want to spring for color, the new OKI 5430n is
awesome at $1250USD. Ethernet or parallel or USB and
very fast and high quality color. For pure greyscale,
the b/w printers do a better job, IMO.

gary g.


At 05:06 PM 4/18/2005, you wrote:


} ---------------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America






From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 07:38:51 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 19 Apr 2005 09:01:07 -0400
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: HUVEC CELLS ON COLLAGEN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

check out papers by William A Muller, et al
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 07:40:09 2005



From: jacqui.ross-at-auckland.ac.nz (by way of MicroscopyListserver)
Date: Tue, 19 Apr 2005 08:02:30 -0500
Subject: [Microscopy] viaWWW: Nikon EclipseNet Time-lapse Data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jacqui.ross-at-auckland.ac.nz) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, April 18, 2005 at 21:16:43
---------------------------------------------------------------------------

Email: jacqui.ross-at-auckland.ac.nz
Name: Jacqui Ross

Organization: The University of Auckland

Title-Subject: [Microscopy] [Filtered] Nikon EclipseNet Time-lapse Data

Question: Dear All,

We are using a TE-2000E running through EclipseNet to do time-lapse imaging in 3 channels.

However, when I try to merge the images in order to then create an AVI movie, I can't seem to do it. I can merge individual images but can't do the whole series at once (as a stack).

Can anyone tell me whether it is possible to do this and if so, how?

Your help would be much appreciated.

Cheers,

Jacqui.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 07:40:38 2005



From: diller-at-stefan-diller.com (by way of MicroscopyListserver)
Date: Tue, 19 Apr 2005 08:03:05 -0500
Subject: [Microscopy] viaWWW: manual for the ImageSlave framegrabber-card

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-stefan-diller.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, April 19, 2005 at 02:15:35
---------------------------------------------------------------------------

Email: diller-at-stefan-diller.com
Name: Stefan Diller

Organization: Photography

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear All,
does anyone have a manual for the ImageSlave framegrabber-card available and can email it to me? Or give me hint where to download it?

Thanks a lot,
Stefan Diller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 11:10:40 2005



From: Alexander Solodukhin :      as4j-at-virginia.edu
Date: Tue, 19 Apr 2005 12:33:04 -0400
Subject: [Microscopy] MM2005 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,



Does somebody know where can I find a list of participants or papers
accepted for

the Microscopy and Microanalysis 2005 Conference?



Many thanks in advance.



Sincerely,



Alexander Solodukhin



Department of Anesthesiology

University of Virginia Health System

P.O.Box 800710

Charlottesville VA 22906-0710

Fax: 434-982-0019

Phone: 434-924-2494




From MicroscopyL-request-at-ns.microscopy.com Tue Apr 19 12:13:41 2005



From: stuart McKernan :      stuartm-at-umn.edu
Date: Tue, 19 Apr 2005 12:36:00 -0500
Subject: [Microscopy] Re: MM2005 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Alexander (and everyone else)

The program is finally in its final state. The list of papers will be
going to Nestor this week and put up on the MSA website. The official
emails from the meeting management will be going out this week also,
now that we have a finalized meeting. We did not want to send out
information with the wrong dates and times for the presentations. It
has taken rather longer than anticipated to get all the sponsoring
societies to agree on the meeting layout.

Having finished we have ended up with the strongest meeting so far with
close to 1100 scientific papers - almost 50% more than previous "good"
meetings!

We look forward to seeing you all in Hawaii.

Stuart McKernan (proceedings editor)

On Apr 19, 2005, at 11:33 AM, Alexander Solodukhin wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Dear Listers,
}
}
}
} Does somebody know where can I find a list of participants or papers
} accepted for
}
} the Microscopy and Microanalysis 2005 Conference?
}
}
}
} Many thanks in advance.
}
}
}
} Sincerely,
}
}
}
} Alexander Solodukhin
}
}
}
} Department of Anesthesiology
}
} University of Virginia Health System
}
} P.O.Box 800710
}
} Charlottesville VA 22906-0710
}
} Fax: 434-982-0019
}
} Phone: 434-924-2494
}
}
}
}
Prof. Stuart McKernan
IT Characterization Facility, University of Minnesota            
E-mail :  stuartm-at-umn.edu
12 Shepherd Labs,                                                    
                 Office:         (612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455                    
Lab:            (612) 626-7594






From MicroscopyL-request-at-ns.microscopy.com Wed Apr 20 09:41:47 2005



From: Matt Olszta :      mjo10-at-psu.edu
Date: Wed, 20 Apr 2005 11:49:13 -0400
Subject: [Microscopy] JEOL JEE 4C Vacuum Evaporator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bonjour à tous!

A researcher working in the Chemistry department here at UM has asked
we a question that I wasn't that comfortable answering so I suggested
we could solicit some other opinions on the EM list. My experience with
the list has shown me there are many unselfish folks out there with a
wealth of knowledge. Maybe one day I will have chance to meet a few of
you. Thanks in advance for your comments and suggestions.
Merci,

André

} From: gthorne-at-Ms.UManitoba.CA

All,
We recently resurrected an old JEOL JEE 4C vacuum evaporator for carbon
coating.
We have the instructions and everything works well on the instrument. I was
wondering if anyone who might have used one of these systems in the past could
give some advice on carbon tip geometry and deposition times. It would be
nice
to put down a thick coating of carbon before I start FIB milling.

Matt Olszta, Ph.D.
Materials Research Institute
Penn State University



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 20 13:18:06 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 20 Apr 2005 11:55:33 -0700
Subject: [Microscopy] Re: Osmium as a Fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} 1. Does anybody have experience with how lipids that are unsaturated
} to various degrees fix with osmium tetroxide (incl
} ferrocyanide-reduced osmium).
}
} We find that cytoplasmic lipid-droplets are very electron-dense in
} eicosapentaenoic acid-treated hepatoma-cells, when osmium tetroxide is
} used as a post-fixative. But in oleic acid-treated cells, under the
} same treatment conditions, the lipid droplets do not stain at all.
}
} Thus, does anybody know how any of below fatty acids "stain" with
} osmium tetroxide:
} arachadonic acid (C20; 4 double bonds)
} eicosapentaenoic acid (C20; 5 double bonds)
} stearic acid (C18; no double bonds)
} oleic acid (C18; 1 double bond)
} linoleic acid (C18, 2 double bonds)
} lineolenic acid (C18; 3 double bonds)
}
} 2. What is the mechanism for osmium tetroxide fixing triglycerides?
}
} Know the theory that in Plipid membranes an osmate forms in the
} hydrophobic portion of the membrane and migrates to the hydrophilic
} portions of the membrane. In triglycerides, do osmium esters for
} example cross-link the fatty acyl chains and remain there? Or does
} anybody know of alternative explanations?
}
} 3. What are the best ways to stabilize and stain a thick (~200 nm)
} section for tomography?
}
Dear Gro,
As no true experts have replied, I'll share what I know. It is my
understanding that two of the O's in OsO4 add across a lipid double
bond, and that, if there is another double bond in the appropriate
position, the other two O's will add across that, so that the lipid
molecules will be cross-linked (or, in a single molecule with two
appropriate double bonds, the molecule will be intramolecularly
cross-linked). I have had no experience with ferrocyanide-reduced
osmium. When lipids form bilayers, the double bonds on adjacent lipids
are in good proximity to react with OsO4, so membranes stain well. I
am not surprised that eicosapentaenoic acid would stain heavily, and I
would expect oleic acid to stain, but much more lightly. I speculate
that the lack of staining in oleic acid-treated cells is due to either
no incorporation of oleic acid in the lipid droplets, or to reduction
of the double bond before incorporation, but this is strictly an
uninformed guess. I also guess that each of the lipids you listed will
stain in proportion to the number of double bonds they contain, but I
do not know if there are differences in staining between cis and trans
conformations, nor do I know whether formation of intramolecular
crosslinks affects the staining intensity. OsO4 dissolves in the
hydrophobic part of the membrane, but I have not heard of it migrating
to the hydrophyllic part. I'm pretty sure that it just reacts
covalently with the double bonds and stays there. As far as
stabilization of thick sections for tomography, evaporation of carbon
onto both the support film and the section will provide greater
conductivity. Some labs routinely pre-expose the section to the beam
so that the section will have undergone any major structural changes
before images are obtained, rather than during the imaging; however,
getting consistent tomograms of altered structures seems to me to be a
poor procedure. IMHO, it is much better to get images of the unaltered
sections using a lower dose.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 20 15:17:53 2005



From: James Roberts :      James.Roberts-at-ventura.org
Date: Wed, 20 Apr 2005 13:37:04 -0700
Subject: [Microscopy] Re: viaWWW: Vacuum issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't know if this will help you. But I recently had a somewhat
similar problem with a different instrument. It turned out to be a bad
gauge. I was making the vacuum that I needed but I (or the interlocks)
couldn't tell because the gauge gave faulty readings below a certain
point. Reason for suspecting the gauge was that the turbo pump was
coming to speed in the same time as usual, indicating there was not
excessive drag on it due to a leak.

Jim

James L. Roberts
Firearm & Toolmark Examiner
Ventura Co. Sheriff's Lab
800 S. Victoria Ave.
Ventura, CA. 93009

(805) 477-1947

James.Roberts-at-ventura.org



} } } {wong2u23-at-yahoo.com} 4/18/2005 6:12:53 PM } } }


------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (wong2u23-at-yahoo.com) from
http://www.msa.microscopy.com/MLFormMail.html on Sunday, April 17,
2005 at 00:14:39
---------------------------------------------------------------------------

Email: wong2u23-at-yahoo.com
Name: Vic Wong

Title-Subject: [Microscopy] [Filtered] MListserver: Vacuum issues

Question: We have a Hitachi s-4500 FESEM which has until recently
worked fine. Recently we have had vacuum trouble, unable to get the
specimen chamber to pump down. We have changed the oil in the RP and
the diff pump, but still remain at the same pressure. The problem
occured when I was exchanging a specimen and when I went to pump down,
only the low lamp in the S.C. went on. The SEC has the high lamp on.
We are down until this problem is fixed, any suggestions?

Vic

---------------------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Wed Apr 20 22:17:35 2005



From: cooldotcalm :      cooldotcalm-at-earthlink.net
Date: Wed, 20 Apr 2005 20:38:53 -0700
Subject: [Microscopy] RE: Re: viaWWW: Vacuum issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Vic,

The most common problem I have is the exchange chamber valve seal
leaking. And since it started upon making an exchange that seems
reasonable. Observe the SC vacuum while you vent the SEC and it should
get even worse--or improve when you pump out the SEC--either would
indicate a leaking seal.


Jeff

------------------------------------------------------------------------
---

Email: wong2u23-at-yahoo.com
Name: Vic Wong

Title-Subject: [Microscopy] [Filtered] MListserver: Vacuum issues

Question: We have a Hitachi s-4500 FESEM which has until recently worked
fine. Recently we have had vacuum trouble, unable to get the specimen
chamber to pump down. We have changed the oil in the RP and the diff
pump, but still remain at the same pressure. The problem occured when I
was exchanging a specimen and when I went to pump down, only the low
lamp in the S.C. went on. The SEC has the high lamp on.
We are down until this problem is fixed, any suggestions?

Vic

------------------------------------------------------------------------
---






From MicroscopyL-request-at-ns.microscopy.com Wed Apr 20 22:40:55 2005



From: cooldotcalm :      cooldotcalm-at-earthlink.net
Date: Wed, 20 Apr 2005 21:02:12 -0700
Subject: [Microscopy] JEOL JEE 4C Vacuum Evaporator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Matt,

I use a Denton evaporator but I can't imagine the settings are very
different. I use 3mm carbon rods that are turned down to about 1mm
diameter at the tip. This is the evaporating region. This diameter
requires about 20 to 40 milliamps to evaporate. Typically starts at 20
(keep the sparks to a minimum but still present) and as evaporation
evolves and the carbon sort of collapses on itself --increasing the
cross section--you need to turn the current up until most of that
smaller diameter is evaporated. You don't want to try to evaporate the
3mm section because that would generate too much heat for most specimens
as well as for the instrument hardware. It takes about a minute or so
to evaportate a 3mm lenth of 1mm section. And this produces a film of
100 to 200 Angstroms.

Jeff


-----Original Message-----
} From: Matt Olszta [mailto:mjo10-at-psu.edu]
Sent: Wednesday, April 20, 2005 7:49 AM
To: Microscopy-at-microscopy.com

All,
We recently resurrected an old JEOL JEE 4C vacuum evaporator for
carbon
coating.
We have the instructions and everything works well on the instrument. I
was wondering if anyone who might have used one of these systems in the
past could give some advice on carbon tip geometry and deposition times.
It would be
nice
to put down a thick coating of carbon before I start FIB milling.

Matt Olszta, Ph.D.
Materials Research Institute
Penn State University




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 00:56:15 2005



From: :      Colin.Veitch-at-csiro.au
Date: Thu, 21 Apr 2005 16:17:59 +1000
Subject: [Microscopy] Xenosput coater with Fe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

We have a Xenosput coater which we use to deposit Cr films on samples
for examination in our FESEM. One of my colleagues wants to deposit
some very thin Fe films using the Xenosput. I have had a steel target
made up and replaced the chromium one with it but can't seem to get an
arc to strike.

I'm wondering if it won't work with a magnetic material. (After my
attempts I replaced the Cr target and everything was fine, so I don't
think it is the Xenosput itself.) Has anyone ever tried using a steel
target in one of these things, and if so did they have any success? An
option is to try a stainless steel target and see how that goes is
suppose.

Any help with this would be appreciated.

Cheers.

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 08:53:53 2005



From: Marek Malecki, M.D., Ph.D. :      mmalecki-at-wisc.edu
Date: Thu, 21 Apr 2005 06:16:08 -0500
Subject: [Microscopy] qualification of M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings, All!
I have a dispute with UW administration over the number of publications
generated / year. I would appreciate seeing opinions of people , who went
through similar hurdles with their sponsors.
In particular, are M&M contributions considered peer-reviewed articles or
not? They are longer than 100 words-long ACB, FASEB, SMI abstracts and
longer than many rapid communications. They are also supposed to be
peer-reviewed. The data in them are distilled to the very core of the
communication.

Perhaps posting all Proceedings in complete forms on the Internet would
boost their prestige / recognition?

Cheers,
Marek



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 09:55:13 2005



From: James.Passmore-at-sealedair.com
Date: Thu, 21 Apr 2005 11:18:04 -0400
Subject: [Microscopy] Re: viaWWW: Vacuum issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'll second the possibility of a gauge problem. We've had pretty much the
same problem on our S-4500. Cleaning the Penning gauge solves the problem,
so we schedule a cleaning once a year. Let me know if you can't find
instructions on cleaning.

Jim Passmore
Research Associate
Cryovac, Sealed Air Corporation
james.passmore-at-sealedair.com
864-433-2927 voice
864-433-2205 fax


"James Roberts" {James.Roberts-at-ventura.org} wrote on 04-20-2005 04:37:04
PM:

}
}
}
------------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------

}
} I don't know if this will help you. But I recently had a somewhat
} similar problem with a different instrument. It turned out to be a bad
} gauge. I was making the vacuum that I needed but I (or the interlocks)
} couldn't tell because the gauge gave faulty readings below a certain
} point. Reason for suspecting the gauge was that the turbo pump was
} coming to speed in the same time as usual, indicating there was not
} excessive drag on it due to a leak.
}
} Jim
}
} James L. Roberts
} Firearm & Toolmark Examiner
} Ventura Co. Sheriff's Lab
} 800 S. Victoria Ave.
} Ventura, CA. 93009
}
} (805) 477-1947
}
} James.Roberts-at-ventura.org
}
}
}
} } } } {wong2u23-at-yahoo.com} 4/18/2005 6:12:53 PM } } }
}
}
}
------------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------

}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (wong2u23-at-yahoo.com) from
} http://www.msa.microscopy.com/MLFormMail.html on Sunday, April 17,
} 2005 at 00:14:39
}
---------------------------------------------------------------------------
}
} Email: wong2u23-at-yahoo.com
} Name: Vic Wong
}
} Title-Subject: [Microscopy] [Filtered] MListserver: Vacuum issues
}
} Question: We have a Hitachi s-4500 FESEM which has until recently
} worked fine. Recently we have had vacuum trouble, unable to get the
} specimen chamber to pump down. We have changed the oil in the RP and
} the diff pump, but still remain at the same pressure. The problem
} occured when I was exchanging a specimen and when I went to pump down,
} only the low lamp in the S.C. went on. The SEC has the high lamp on.
} We are down until this problem is fixed, any suggestions?
}
} Vic
}
}
---------------------------------------------------------------------------
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 11:13:23 2005



From: stuart McKernan :      stuartm-at-umn.edu
Date: Thu, 21 Apr 2005 11:34:07 -0500
Subject: [Microscopy] Re: qualification of M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The proceedings of the Microscopy and Microscopy meetings are published
online as a supplement (supplement #2) to the Microscopy and
Microanalysis journal. As such they are available online through the
CUP online journals website: journals.cambridge.org. These supplements
are available free-of-charge to anyone with internet access.

On this matter I would greatly welcome comments to this problem (which
is not an isolated one) to be directed (or at least copied) to me. We
are again in the process of re-evaluating the usefulness of these
papers in their present format to the scientific community, and would
welcome input from as many different areas as possible - Communities
that use them in publication counts and those that are precluded from
using them for that reason for example, as well as individual opinions.


Stuart McKernan (Proceedings editor)


On Apr 21, 2005, at 6:16 AM, Marek Malecki, M.D., Ph.D. wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Greetings, All!
} I have a dispute with UW administration over the number of
} publications generated / year. I would appreciate seeing opinions of
} people , who went through similar hurdles with their sponsors.
} In particular, are M&M contributions considered peer-reviewed articles
} or not? They are longer than 100 words-long ACB, FASEB, SMI abstracts
} and longer than many rapid communications. They are also supposed to
} be peer-reviewed. The data in them are distilled to the very core of
} the communication.
}
} Perhaps posting all Proceedings in complete forms on the Internet
} would boost their prestige / recognition?
}
} Cheers,
} Marek
}
}
}
Prof. Stuart McKernan
IT Characterization Facility, University of Minnesota            
E-mail :  stuartm-at-umn.edu
12 Shepherd Labs,                                                    
                 Office:         (612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455                    
Lab:            (612) 626-7594






From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 12:41:48 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 21 Apr 2005 13:03:06 -0500
Subject: [Microscopy] Re: qualification of M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marek,
For what it is worth, I do not consider M&M abstracts as peer reviewed. A
true peer reviewed article is one that is independently reviewed by 2 or
more scientists knowledgeable about the appropriate field as required by the
contents of the paper. Unless I am misinformed, I believe that M&M abstracts
are checked for layout, appropriate format and submission information but
are not judged on content.

I personally assume that all abstracts, regardless of length, do not contain
sufficient data, discussion, and conclusions for a reader to make an
informed opinion as to the overall merits of the research.

An abstract is sufficient to let you know if the subject matter is of
sufficient interest to you to want to learn more. In that case you read the
entire paper, listen to the presentation, or read the poster to get the
"rest of the story". Then you can begin to make critical judgements as to
the merits of the research.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy




On 4/21/05 6:16 AM, "Marek Malecki, M.D., Ph.D." {mmalecki-at-wisc.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Greetings, All!
} I have a dispute with UW administration over the number of publications
} generated / year. I would appreciate seeing opinions of people , who went
} through similar hurdles with their sponsors.
} In particular, are M&M contributions considered peer-reviewed articles or
} not? They are longer than 100 words-long ACB, FASEB, SMI abstracts and
} longer than many rapid communications. They are also supposed to be
} peer-reviewed. The data in them are distilled to the very core of the
} communication.
}
} Perhaps posting all Proceedings in complete forms on the Internet would
} boost their prestige / recognition?
}
} Cheers,
} Marek
}
}




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 14:06:42 2005



From: Bob Price :      PRICE-at-gw.med.sc.edu (by way of MicroscopyListserver)
Date: Thu, 21 Apr 2005 21:24:26 -0500
Subject: [Microscopy] [Filtered] Fwd: REJECTED MAIL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colin,
You will find that each different material has a different work function and
so you must find the different conditions that will sputter that material.
Fe may have a higher work function than Cr, so you might need a higher
voltage or current to sputter it. I would use plain carbon steel, because
stainless steel is an alloy that has other materials in it, so you would not
get a pure Fe film. Good luck.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: {Colin.Veitch-at-csiro.au}
To: {Microscopy-at-msa.microscopy.com}
Sent: Wednesday, April 20, 2005 11:17 PM



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Marek and others,

As one of the few (as far as I know the only one) to be foolish enough
to be Program Chair for two M&M meetings and responsible for much of the
content of the scientific content of the M&M 2001 and 2005 Proceedings I
felt I should weigh in on this one.

For as long as I have been active on MSA Program Committees (1997
through 2005) the debate concerning how to list M&M meeting
contributions on one's CV or annual evaluations has been a hot topic in
MSA council and program production meetings.

Marek raises a valid point that to meet the format for contributions,
MSA abstracts (some call them papers), need to be two pages in length
which is significantly different than any other major biology oriented
meetings I am familiar with. This is certainly longer than the 100 words
we get for FASEB or Cell Biology abstracts. The 2 page requirement was
essential in the age of the MSA hard copy proceedings so all papers were
printed on facing pages. If a paper was only a single page, this
disrupted the printing and organization of the proceedings. However, as
program committee members, if a single line of text was on the second
page of the paper it was deemed acceptable based on format. As we have
moved to the electronic format of CD for all submissions and hard copy
Proceedings for selected representative submissions, we have been able
to relax this standard to some extent. Now, in some cases, submissions
of less than 2 pages are accepted but they are limited to publication on
the CD only and are not eligible for selection to be printed in the hard
copy proceedings. The discussions concerning the 2-page format that have
been held during MSA council meetings are much too long to review in
this email, but I am sure if people attend MSA council meetings where
this topic is discussed they would know it is a frequent and hot topic
for debate.

Are submissions peer reviewed?? They are to the extent that there is
one member of the Program Committee responsible for looking at
formatting and scientific content of the contribution. This is typically
the session chair. If there are any questions concerning scientific
content the contribution is passed on to at least one other person with
knowledge of the scientific discipline. The paper and reviewer comments
are then given to the Program Chair or Vice Chair, depending on if the
contribution is biological or physical sciences, who makes the final
decision. Reasons for rejection include fraud or other ethical problems
such as improper use of animals, questionable data, duplication of
existing published data, minimal scientific content, etc. Often, if a
reviewer has a problem, the discussion centers on if the problem is
egregious enough to warrant outright rejection or if the author should
be given the chance at a poster presentation to defend his/her data.

I hope this helps clarify some of the process. I am sure Stuart
McKernan as editor of the Proceedings and Charlie Lyman as Editor in
Chief of Microscopy and Microanalysis would love to receive comments
from the membership about this topic.

Bob




Robert L. Price, PhD
Research Professor and
Director, Instrumentation Resource Facility
Dept. Developmental Biology and Anatomy
School of Medicine, USC
Bldg 1 Room B60
6439 Garner's Ferry Road
Columbia, SC 29208

Fax: 803-733-1533
Telephone: 803-733-3392


} } } } "Marek Malecki, M.D., Ph.D." {mmalecki-at-wisc.edu} 04/21/05 7:16 AM
} } } }
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Greetings, All!
} I have a dispute with UW administration over the number of publications
}
} generated / year. I would appreciate seeing opinions of people , who
} went
} through similar hurdles with their sponsors.
} In particular, are M&M contributions considered peer-reviewed articles
} or
} not? They are longer than 100 words-long ACB, FASEB, SMI abstracts and
}
} longer than many rapid communications. They are also supposed to be
} peer-reviewed. The data in them are distilled to the very core of the
} communication.
}
} Perhaps posting all Proceedings in complete forms on the Internet would
}
} boost their prestige / recognition?
}
} Cheers,
} Marek
}
}


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 22:39:41 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 21 Apr 2005 23:02:04 -0500
Subject: [Microscopy] Re: qualification of MM proceedings :Scientific Review of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debby

You are both misinformed and incorrect in your assertion
concerning the M&M submissions.

M&M submissions are certainly reviewed for content, as each paper
is vetted by at least one member of the program committee having
familiarity of the topic under which they were submitted,
usually this is a symposium organizer, who is certainly familiar with
the field.

As a former Program Chair and member of the Program Committee
for well over a decade I can confirm that the scientific content is considered and I have
rejected non-scientific content submissions. This year I personally read and
reviewed the content of over 150 submissions to the 2005 meeting.

I also refer you to the Instructions for Authors which states unambiguously

"Papers should be a condensed version of the final presentation and
include all significant findings. Write the text so that readers who are
not specialists can appreciate the purpose of the study and understand the
procedures and conclusions."

It is also true that the scientific content and merit varies widely in various
submissions, and I certainly don't always agree with the content,
but that is a different issue. A large number of these
short papers (yes, I consider them papers when they they
contain significant results) document results of experiments
and conclusions which are important in their own right. It is also
true that some are abstracts/summaries of preliminary work, while
others are simply extended abstracts. This varies from
author to author and when you consider there may be 700-1000
submissions to the proceedings this variablity is not surprizing.

Please remember , what you consider an abstract, others consider a
short/rapid publication. The difference depends upon the content and the
field, not on the format or number of "reviewers". Dave Piston and I
have argued this at great lengths during various meetings (as well as over a few
beers). By different fields, here I mean Life Science vs Materials Science, as these
can sometimes have vastly different time scales and criteria. While it may
be possible to get a short paper published in the Life Sciences in six months,
in many cases it can take 12-18 months in some Materials areas.
Judging the merits of a publication solely on its length and the number of
reviewers who have read it prior to publication is not, in my opinion, logical.
A work is citable when it contains substantial verifiable results and has been vetted by
an outside reviewer (thus the M&M proceedings meets a minimum criteria). This
being said, ultimately the true test of the merits of a publication becomes
when its data is referenced or is the basis for other publications.

I might add that I personally preferred the format which MSA dropped years ago
which allowed "4 page" submissions . At least 1/2 of the time I find it difficult to
fit everything into the 2 page format. While a number of individuals are pushing
to decrease the length of submissions (i.e. permitting 1 page submissions for the proceedings)
I personally consider this the wrong way to go and instead argue that we should
continue with the existing format and also be allowing slightly longer
works. I use the MM proceedings as a rapid publication venue for new results,
and then turn to a Journal like "Microscopy & Microanalysis" for longer manuscripts,
into which one can integrate a larger body of work.

My 2 cents.....

Nestor
Your Friendly Neighborhood SysOp.




} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 22:42:38 2005



From: Bob Price :      PRICE-at-gw.med.sc.edu (by way of MicroscopyListserver)
Date: Thu, 21 Apr 2005 23:04:42 -0500
Subject: [Microscopy] Re: qualification of M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Marek and others,

As one of the few (as far as I know the only one) to be foolish enough
to be Program Chair for two M&M meetings and responsible for much of the
content of the scientific content of the M&M 2001 and 2005 Proceedings I
felt I should weigh in on this one.

For as long as I have been active on MSA Program Committees (1997
through 2005) the debate concerning how to list M&M meeting
contributions on one's CV or annual evaluations has been a hot topic in
MSA council and program production meetings.

Marek raises a valid point that to meet the format for contributions,
MSA abstracts (some call them papers), need to be two pages in length
which is significantly different than any other major biology oriented
meetings I am familiar with. This is certainly longer than the 100 words
we get for FASEB or Cell Biology abstracts. The 2 page requirement was
essential in the age of the MSA hard copy proceedings so all papers were
printed on facing pages. If a paper was only a single page, this
disrupted the printing and organization of the proceedings. However, as
program committee members, if a single line of text was on the second
page of the paper it was deemed acceptable based on format. As we have
moved to the electronic format of CD for all submissions and hard copy
Proceedings for selected representative submissions, we have been able
to relax this standard to some extent. Now, in some cases, submissions
of less than 2 pages are accepted but they are limited to publication on
the CD only and are not eligible for selection to be printed in the hard
copy proceedings. The discussions concerning the 2-page format that have
been held during MSA council meetings are much too long to review in
this email, but I am sure if people attend MSA council meetings where
this topic is discussed they would know it is a frequent and hot topic
for debate.

Are submissions peer reviewed?? They are to the extent that there is
one member of the Program Committee responsible for looking at
formatting and scientific content of the contribution. This is typically
the session chair. If there are any questions concerning scientific
content the contribution is passed on to at least one other person with
knowledge of the scientific discipline. The paper and reviewer comments
are then given to the Program Chair or Vice Chair, depending on if the
contribution is biological or physical sciences, who makes the final
decision. Reasons for rejection include fraud or other ethical problems
such as improper use of animals, questionable data, duplication of
existing published data, minimal scientific content, etc. Often, if a
reviewer has a problem, the discussion centers on if the problem is
egregious enough to warrant outright rejection or if the author should
be given the chance at a poster presentation to defend his/her data.

I hope this helps clarify some of the process. I am sure Stuart
McKernan as editor of the Proceedings and Charlie Lyman as Editor in
Chief of Microscopy and Microanalysis would love to receive comments
from the membership about this topic.

Bob




Robert L. Price, PhD
Research Professor and
Director, Instrumentation Resource Facility
Dept. Developmental Biology and Anatomy
School of Medicine, USC
Bldg 1 Room B60
6439 Garner's Ferry Road
Columbia, SC 29208

Fax: 803-733-1533
Telephone: 803-733-3392


} } } } "Marek Malecki, M.D., Ph.D." {mmalecki-at-wisc.edu} 04/21/05 7:16 AM
} } } }
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Greetings, All!
} I have a dispute with UW administration over the number of publications
}
} generated / year. I would appreciate seeing opinions of people , who
} went
} through similar hurdles with their sponsors.
} In particular, are M&M contributions considered peer-reviewed articles
} or
} not? They are longer than 100 words-long ACB, FASEB, SMI abstracts and
}
} longer than many rapid communications. They are also supposed to be
} peer-reviewed. The data in them are distilled to the very core of the
} communication.
}
} Perhaps posting all Proceedings in complete forms on the Internet would
}
} boost their prestige / recognition?
}
} Cheers,
} Marek
}
}


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 22:47:06 2005



From: Judith_A_Ruiz-at-whirlpool.com (by way of MicroscopyListserver)
Date: Thu, 21 Apr 2005 23:09:10 -0500
Subject: [Microscopy] viaWWW: microtome polypropylene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Judith_A_Ruiz-at-whirlpool.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, April 19, 2005 at 12:56:18
---------------------------------------------------------------------------

Email: Judith_A_Ruiz-at-whirlpool.com
Name: Judy

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I'm trying to microtome various polypropylene pieces too narrow to place in the vice on the microtome. I need to slice the surface, not the cross-section. So, I was wondering if anyone has advice on the best way to mount this sample, in what kind of resin? And what kind of mold. the samples are going to be all shapes and sizes.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 22:47:31 2005



From: duleyml-at-muohio.edu (by way of MicroscopyListserver)
Date: Thu, 21 Apr 2005 23:09:35 -0500
Subject: [Microscopy] viaWWW: carbon adhesive tabs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (duleyml-at-muohio.edu) from http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April 19, 2005 at 14:23:24
---------------------------------------------------------------------------

Email: duleyml-at-muohio.edu
Name: Matthew L. Duley

Organization: Miami University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: We are experiencing serious problems with carbon adhesive tabs. We have tried three different vendors and they are all the same. The surface is very rough with numerous bubbles, cracks and pock marks. We are currently doing a lot of particle (50 microns to 1mm) analysis, and imaging including EDS and EBSD and weíre in desperate need of good carbon tabs or an alternative solution. There was a short discussion on the Microscopy List server where a vendor suggested the users should use "new" tabs. However, we are currently using a very limited supply of tabs that are 3+ years old because they are the only ones that are smooth. Surely, we arenít the only facility having this problem. Has anyone found a solution to this?


Sincerely,

Matthew L. Duley
Electron Microscopist
Electron Microscope Facility
Miami University
Oxford, Ohio 45056

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 22:48:16 2005



From: labtechcorp-at-nji.com (by way of MicroscopyListserver)
Date: Thu, 21 Apr 2005 23:10:05 -0500
Subject: [Microscopy] viaWWW: ultra-thin window repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (labtechcorp-at-nji.com) from http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April 19, 2005 at 14:35:30
---------------------------------------------------------------------------

Email: labtechcorp-at-nji.com
Name: Charles Schwartz

Organization: Labtech Corp.

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Does anyone know where I can get the ultra-thin window on my old Tracor EDX system repaired without having to mortgage the business? NJ/East Coast location preferable

Charlie

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 22:49:15 2005



From: raynald.gauvin-at-mcgill.ca (by way of MicroscopyListserver)
Date: Thu, 21 Apr 2005 23:11:03 -0500
Subject: [Microscopy] viaWWW: Workshop in SEM related techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (raynald.gauvin-at-mcgill.ca) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, April 20, 2005 at 15:01:11
---------------------------------------------------------------------------

Email: raynald.gauvin-at-mcgill.ca
Name: Raynald Gauvin

Organization: McGill University

Title-Subject: [Microscopy] [Filtered] MListserver: Workshop in SEM related techniques

Question:

} From May 9 to 13 2005, a workshop about advanced techniques in SEM and microanalysis for materials characterization will be held at McGill University, Montreal, Canada. For more detalls, please go to:

http://www.mcgill.ca/minmet/ebeamworkshop/

Please note that there is a maximum number of participants and there is still few places that are available.

Very best regards

Raynald GAUVIN

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 22:49:35 2005



From: bdrysdale-at-fibrolight.com (by way of MicroscopyListserver)
Date: Thu, 21 Apr 2005 23:11:39 -0500
Subject: [Microscopy] viaWWW: Dr Raymond Rife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bdrysdale-at-fibrolight.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, April 20, 2005 at 15:32:43
---------------------------------------------------------------------------

Email: bdrysdale-at-fibrolight.com
Name: Robert Drysdale

Organization: Fibrolight Technology Inc

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Greetings

Has the MSA or any affiliated members out there have any information on a Dr Raymond Rife, other than what's available
on the net. He seems to be quite an interesting individual. Any info would be appreciated.

Best regards
R Drysdale


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 21 22:50:05 2005



From: mauricio.bravo-at-dalsemi.com (by way of MicroscopyListserver)
Date: Thu, 21 Apr 2005 23:12:09 -0500
Subject: [Microscopy] viaWWW: Sputter coater targets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mauricio.bravo-at-dalsemi.com) from http://www.msa.microscopy.com/MLFormMail.html on Thursday, April 21, 2005 at 15:59:21
---------------------------------------------------------------------------

Email: mauricio.bravo-at-dalsemi.com
Name: Mauricio Bravo

Organization: Microscopy Lab Supervisor at Dallas Semiconductor

Title-Subject: [Microscopy] [Filtered] Sputter coater targets

Question: Hi,

I need to replace the Gold target in a Ernest Fullan EMS-76M sputter coater. The question I have is "Should I be looking into buying a Gold-Palladium target instead?" Would a 40%Au-60%Pd target help me get good images? How about a %60Au-40%Pd? I understand that the benefit of the allow is that allows for smaller grain size while maintaining good secundary electron emission.

Many thanks in advance for your time.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 06:55:46 2005



From: Sven Terclavers :      Sven.Terclavers-at-med.kuleuven.be
Date: Fri, 22 Apr 2005 14:17:20 +0200 (Romance Daylight Time)
Subject: [Microscopy] Reservation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

I'm in search for a good, preferably online, software program for users to
book a microscope.
At the moment, I made a shared excel-workbook per microscope with the tabs
as months and where users can book a microscope per hour. This excel-file is
available through the local network (intranet) only. However, I'm sure there
are better options and even some which allow to go online and book over the
internet. Can anyone help me with this?
Thanks in advance,

Sven Terclavers


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 07:09:31 2005



From: Gerroir, Paul :      paul.gerroir-at-xrcc.xeroxlabs.com
Date: Fri, 22 Apr 2005 08:29:43 -0400
Subject: [Microscopy] viaWWW: carbon adhesive tabs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Matthew,
I too have voiced concern regarding this problem and have received the same advice; "Use only new tabs" and "Store in a fridge until they are needed". The problem persists. It seems rather odd to me that the older tabs were much better and there were no similar precautions issued with regard to their age or storage requirements.

Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: 905-823-7091, ext.216
FAX: 905-822-7022
e-mail: paul.gerroir-at-xrcc.xeroxlabs.com

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:duleyml-at-muohio.edu]
Sent: Friday, April 22, 2005 12:10 AM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (duleyml-at-muohio.edu) from http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April 19, 2005 at 14:23:24
---------------------------------------------------------------------------

Email: duleyml-at-muohio.edu
Name: Matthew L. Duley

Organization: Miami University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: We are experiencing serious problems with carbon adhesive tabs. We have tried three different vendors and they are all the same. The surface is very rough with numerous bubbles, cracks and pock marks. We are currently doing a lot of particle (50 microns to 1mm) analysis, and imaging including EDS and EBSD and weíre in desperate need of good carbon tabs or an alternative solution. There was a short discussion on the Microscopy List server where a vendor suggested the users should use "new" tabs. However, we are currently using a very limited supply of tabs that are 3+ years old because they are the only ones that are smooth. Surely, we arenít the only facility having this problem. Has anyone found a solution to this?


Sincerely,

Matthew L. Duley
Electron Microscopist
Electron Microscope Facility
Miami University
Oxford, Ohio 45056

---------------------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 07:45:35 2005



From: Engle, Mary :      mgengle-at-uky.edu
Date: Fri, 22 Apr 2005 09:07:25 -0400
Subject: [Microscopy] cutting polypropylene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Judith,
I have been fairly successful with similar samples by embedding them in a flat embedding capsule in an epoxy resin such as LX112. Before the advent of those capsules I used hinged beem capsules by turning the capsule upside down and cutting the pointed end off. With either one, you'll be able to cut the surface of the material.
Good luck,
Mary Gail


Mary Gail Engle
Sr. Research Laboratory Manager
Electron Microscopy & Imaging Facility
HSRB Rm 001
University of Kentucky
Lexington, KY 40536-0305
Phone (859) 323-6108
Fax (859) 257-9700





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 07:46:02 2005



From: aquaseedfishtecknik-at-yahoo.com (by way of MicroscopyListserver)
Date: Fri, 22 Apr 2005 08:08:04 -0500
Subject: [Microscopy] viaWWW: research in Daphnia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (aquaseedfishtecknik-at-yahoo.com) from http://www.microscopy.com/MLFormMail.html on Friday, April 22, 2005 at 04:27:59
---------------------------------------------------------------------------

Email: aquaseedfishtecknik-at-yahoo.com
Name: kehinde samuel

Title-Subject: [Microscopy] [Filtered] research in Daphnia

Question: please send all information about Daphnia and it's picture or mivies

please do we require written permission to use your Daphnia picture in our work work

thanks,
kenny.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 08:11:10 2005



From: David Kempson :      kempson-at-geol.queensu.ca
Date: Fri, 22 Apr 2005 09:35:56 -0400
Subject: [Microscopy] SEM -- AMRAY 1830

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are about to receive a donated AMRAY 1830 SEM . I need to know the
physical size,weight and room requirements including vibration, and stray
AC fields measurements for this machine. I am assuming that 220 AC power is
required but have no idea of the current. I have searched the web but can't
find much about these Items.

Thanks in advance


Dave Kempson
Queen's University
Geology Department
613-533-2595




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 08:50:43 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 22 Apr 2005 10:08:41 -0400
Subject: [Microscopy] Re: viaWWW: Dr Raymond Rife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Oh please, not Raymond Rife again. I don't think that the Great Man
was a doctor, MD, DDS, PhD, JD or otherwise. I don't think you will find
any of his work published, it seems that most of his claims were just
that, claims. No evidence to support them. I have read some of what is
on the web, be sure to read the articles all the way to the end. Seems
that while there is lots of glowing praise at the beginning of several
articles by the time one gets to the end the author realizes that there
is no real evidence to support the claims. There is some author (in
Canada?) who insists that Rife cured cancer but the Evil Establishment
covered up, ignored, etc. (fill in your favorite conspiracy theory here)
the Great Man's contributions.
Bottom line, it is all BS. Rife was a gadget freak who could not
tell optical aritfacts from real biological structure.

Geoff

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************

by way of MicroscopyListserver wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 09:03:48 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 22 Apr 2005 07:24:55 -0700
Subject: [Microscopy] Re: viaWWW: ultra-thin window repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If the window is broken, the vacuum in the detector
is certainly gone. You need new window and pump out
of detector and fresh getter. It will probably need
a new support grid.

Not a cheap prospect.

gary g.


At 09:10 PM 4/21/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 09:08:19 2005



From: Iain Miller :      milleri-at-ohio.edu
Date: Fri, 22 Apr 2005 10:29:25 -0400
Subject: [Microscopy] Reservation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sven,

phpScheduleIt is a very easy to use online scheduler for any resource,
microscope or other wise. Users can book time, cancel time, amend booking
etc online from anywhere. It is open source software available for download
from http://www.php.brickhost.com/

Iain Miller
Department of Biological Sciences
Ohio University
Athens, OH 45701

740-593-2120


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 09:09:35 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 22 Apr 2005 07:30:58 -0700
Subject: [Microscopy] Re: viaWWW: carbon adhesive tabs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Check the archives. This was discussed quite a few months
ago. The issue at that point was high resistance and
too thick of tabs.

Pella, EMS and SPI all seem to have solved this. I know from
use that Pella and EMS tabs are low resistance, smooth and sticky.
I have not tried the SPI tabs. You should buy new tabs.

The Pella and EMS (Nissin) tabs do exhibit holes and tears
when put in the sputter coater. The only way I've seen to
reduce this is to coat fast at higher current or longer
at very reduced current. It is either the vacuum or plasma
that degrades their surface...or, perhaps both.

gary g.


At 09:09 PM 4/21/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 10:08:00 2005



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Fri, 22 Apr 2005 11:29:24 -0400
Subject: [Microscopy] microtome polypropylene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This can be tricky since you need to slice surface vs. cross-section. I'll
be interested in what suggestions others might have as this has also been a
problem for me. If the samples are not thin films you can use a room temp
cure epoxy such as epo-fix, fill a flat mold, wait until is has nearly
cured and then press your sample into the epoxy oriented as need be, this
way it shouldn't move during remaining cure. You have to be careful that
the sample doesn't pop out when trimming which happens to me if the sample
is to thin. One way I've gotten around this is to epoxy the thin film to a
smaller epoxy block and then embed this into regular flat mold. Other
ideas? Good luck Judith. By the way I assume you are using a
cryo-ultramicrotome. Steve

Question: I'm trying to microtome various polypropylene pieces too narrow
to place in the vice on the microtome. I need to slice the surface, not
the cross-section. So, I was wondering if anyone has advice on the best
way to mount this sample, in what kind of resin? And what kind of mold.
the samples are going to be all shapes and sizes.


Stephen McCartney
Research Associate
Macromolecules and Interfaces Institute
2108 Hahn Hall
Va Tech
Blacksburg, VA 24061
540-231-9765 - phone
540-231-8517 - FAX



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 10:21:15 2005



From: pgrover :      pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 22 Apr 2005 10:43:08 -0500
Subject: [Microscopy] Royal Rife Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

C'mon, people. Give the poor man a break. Who among us can go to
http://www.medicaltruth.com/rife/RoyalRife.html, gaze upon the Rife
Universal Microscope and doubt that it can defy the laws of physics?

Paul

----------------------------------------------
"'Why listen, Lady,' he said with a grin of delight 'the monks of old
slept in their coffins!' 'They wasn't as advanced as we are.' the old
woman said." - The Life You Save May be Your Own






From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 10:54:46 2005



From: Evgenia Pekarskaya :      pekarskaya-at-mail.pse.umass.edu
Date: Fri, 22 Apr 2005 12:16:15 -0400
Subject: [Microscopy] Reservation software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sven,

We are using Calcium software from http://www.brownbearsoftware.com/.
Except for a few minor things we are quite happy with it. However, it is not
free unfortunately.

Evgenia

========================
Evgenia Pekarskaya
Keck Electron Microscopy Laboratory
Polymer Science & Engineering
UMass, Amherst; Conte A229
Tel. 413 545 2261
E-fax 325 202 7338
http://www.umassmicroscopy.com/
========================

-----Original Message-----
} From: Sven Terclavers [mailto:Sven.Terclavers-at-med.kuleuven.be]
Sent: Friday, April 22, 2005 8:17 AM
To: Microscopy-at-msa.microscopy.com

Dear colleagues,

I'm in search for a good, preferably online, software program for users to
book a microscope.
At the moment, I made a shared excel-workbook per microscope with the tabs
as months and where users can book a microscope per hour. This excel-file is
available through the local network (intranet) only. However, I'm sure there
are better options and even some which allow to go online and book over the
internet. Can anyone help me with this?
Thanks in advance,

Sven Terclavers





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 10:56:48 2005



From: Ken Converse :      kenconverse-at-qualityimages.biz
Date: Fri, 22 Apr 2005 12:17:54 -0400
Subject: [Microscopy] SEM -- AMRAY 1830

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dave,
Since you're from Canada, I'm assuming that the donation is from somewhere
in North America. Unless it was special ordered, it should be 120VAC, 60
Hz.

I've looked through my materials and can't find any installation
instructions, room specs or instrument specs but if you can grab a piece of
floor at least 9' x 7', that should give you enough room for the instrument,
the operator and servicing. Larger would be better, especially from a
servicing standpoint.

The weight is probably in the range of 1000-1200 lbs, the largest current
draw will be the mechanical pump with a 1/2 hp motor drawing 5.5 to 9.5A,
depending upon the efficiency of the motor, air and water will be needed if
it has a diffusion pump but probably not if it has a turbo pump. Low
frequency vibration ( {20 Hz) is the most critical and the specs are given in
different ways by different manufacturers. One spec used is not more than
10 micro-inches displacement in any axis. Fields can have a little
flexibility. One manufacturer's spec is not more than 10 milligaus, but if
you're not doing electron beam lithography, larger fields at 60 Hz can be
tolerated because the scan rate is sync'd to line frequency when taking
micrographs. Other frequencies must remain within spec.

Hopefully someone out in lister land has a copy of Amray's specs and room
layout and can get them to you.

Ken Converse

owner 
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
} From: David Kempson [mailto:kempson-at-geol.queensu.ca]
Sent: Friday, April 22, 2005 9:36 AM
To: Microscopy-at-microscopy.com

We are about to receive a donated AMRAY 1830 SEM . I need to know the
physical size,weight and room requirements including vibration, and stray
AC fields measurements for this machine. I am assuming that 220 AC power is
required but have no idea of the current. I have searched the web but can't
find much about these Items.

Thanks in advance


Dave Kempson
Queen's University
Geology Department
613-533-2595








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From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 13:37:36 2005



From: Becky Holdford :      r-holdford-at-ti.com
Date: Fri, 22 Apr 2005 13:58:45 -0500
Subject: [Microscopy] Re: viaWWW: Sputter coater targets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mauricio: I would definitely switch to Au-Pd as it does give a smaller
deposited grain size for the same sputter conditions. I use Au-Pd but
I'm uncertain as to the alloy. It's the standard one all the suppliers
have. But if I needed to to coat for really high mag/resolution work
(} 100,000X) I would use iridium.

by way of MicroscopyListserver wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 14:53:57 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Fri, 22 Apr 2005 13:14:27 -0700
Subject: [Microscopy] Re: qualification of M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debbie;
Peer review? Each of my abstracts get reviewed by my manager, my
manager's manager, my manager's manager's manager, a corporate attorney,
and then my manager's manager's manager's manager, who is a vice
president. And then, I have to do it all over again with the
presentation foils for each talk. Isn't anybody outside of MSA checking
your work?
As for MSA reviews, perhaps you do not recall an era when any
MSA abstract that was submitted would be accepted. That ended abruptly
after the publication of the infamous "Dappled Field" hoax abstract in
1974 (page 552-I still take it out and look at it occasionally when I
need a laugh).

John Mardinly
Intel

These comments are the opinion of the author and do not represent the
opinion of Intel Corporation.

-----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: Thursday, April 21, 2005 11:03 AM
To: Marek Malecki, M.D., Ph.D.; microscopy-at-microscopy.com

Marek,
For what it is worth, I do not consider M&M abstracts as peer reviewed.
A
true peer reviewed article is one that is independently reviewed by 2 or
more scientists knowledgeable about the appropriate field as required by
the
contents of the paper. Unless I am misinformed, I believe that M&M
abstracts
are checked for layout, appropriate format and submission information
but
are not judged on content.

I personally assume that all abstracts, regardless of length, do not
contain
sufficient data, discussion, and conclusions for a reader to make an
informed opinion as to the overall merits of the research.

An abstract is sufficient to let you know if the subject matter is of
sufficient interest to you to want to learn more. In that case you read
the
entire paper, listen to the presentation, or read the poster to get the
"rest of the story". Then you can begin to make critical judgements as
to
the merits of the research.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy




On 4/21/05 6:16 AM, "Marek Malecki, M.D., Ph.D." {mmalecki-at-wisc.edu}
wrote:

}
}
}
------------------------------------------------------------------------
------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
------}
-
}
} Greetings, All!
} I have a dispute with UW administration over the number of
publications
} generated / year. I would appreciate seeing opinions of people , who
went
} through similar hurdles with their sponsors.
} In particular, are M&M contributions considered peer-reviewed articles
or
} not? They are longer than 100 words-long ACB, FASEB, SMI abstracts and
} longer than many rapid communications. They are also supposed to be
} peer-reviewed. The data in them are distilled to the very core of the
} communication.
}
} Perhaps posting all Proceedings in complete forms on the Internet
would
} boost their prestige / recognition?
}
} Cheers,
} Marek
}
}






From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 15:50:50 2005



From: William H Roberts :      William.H.Roberts-at-USA.dupont.com
Date: Fri, 22 Apr 2005 17:11:59 -0400
Subject: [Microscopy] SEM/TEM/LM - Cryomicrotomy Course Wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone out there have a recommendation for a short course or other
access to instruction on cryoultramicrotomy of polymers? I've seen a few
advertisements for courses, but I'm reluctant to commit the funds without a
reasonable expectation of the content of the course and the quality of the
instruction. I have inherited a Reichert-Jung Ultracut E with the FC-4E
Cryo accessory and I would like to have some background before I jump in
and destroy a new diamond knife or the instrument itself.

TIA,

Bill



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From MicroscopyL-request-at-ns.microscopy.com Fri Apr 22 20:45:49 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sat, 23 Apr 2005 14:07:07 +1200
Subject: [Microscopy] Re: viaWWW: ultra-thin window repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Jim Nicolino's Advanced Analysis Technologies (www.advancedanalysistech.com)
has an office in Monroeville, PA.

That's closeish, isn't it?

Jim's email:

JNicolino-at-advancedanalysistech.com

cheers

rtch

} }
} } Email: labtechcorp-at-nji.com
} } Name: Charles Schwartz
} }
} } Organization: Labtech Corp.
} }
} } Title-Subject: [Microscopy] [Filtered] MListserver:
} }
} } Question: Does anyone know where I can get the ultra-thin window on
} } my old Tracor EDX system repaired without having to mortgage the
} } business? NJ/East Coast location preferable
} }
} } Charlie
} }
} } ---------------------------------------------------------------------
} } ------
}
}

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Sat Apr 23 00:34:07 2005



From: Scott Walck on Comcast :      swalck-at-comcast.net
Date: Sat, 23 Apr 2005 01:52:27 -0400
Subject: [Microscopy] Xenosput coater with Fe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colin, Not being familiar with the Xenosput system, I looked it up to see
whether it was a magnetron sputter system. Yep, it was. If you have a
ferromagnetic material, it will not sputter because it screws up (And that
is a technical phrase!) the magnetic field lines that guides the ions to the
target for sputtering. It is the presence of the magnetic lines that gives
you the little racetrack patterns that appear on targets when you use them.
You can get a thin Fe film by ion sputtering with no problems. Just like
the Cr target, you should sputter away the oxide coating while shielding
your sample prior to actually coating.

Disclaimer:
South Bay Technology manufactures and sells the IBS/e ion beam sputter unit
for applying uniform thin coatings on samples and substrates by ion
sputtering. If you visit our website, www.southbaytech.com, you can find
our representative in Australia.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127

-----Original Message-----
} From: Colin.Veitch-at-csiro.au [mailto:Colin.Veitch-at-csiro.au]
Sent: Thursday, April 21, 2005 2:18 AM
To: Microscopy-at-msa.microscopy.com

Hi All,

We have a Xenosput coater which we use to deposit Cr films on samples
for examination in our FESEM. One of my colleagues wants to deposit
some very thin Fe films using the Xenosput. I have had a steel target
made up and replaced the chromium one with it but can't seem to get an
arc to strike.

I'm wondering if it won't work with a magnetic material. (After my
attempts I replaced the Cr target and everything was fine, so I don't
think it is the Xenosput itself.) Has anyone ever tried using a steel
target in one of these things, and if so did they have any success? An
option is to try a stainless steel target and see how that goes is
suppose.

Any help with this would be appreciated.

Cheers.

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.







From MicroscopyL-request-at-ns.microscopy.com Sat Apr 23 01:02:41 2005



From: Scott Walck on Comcast :      swalck-at-comcast.net
Date: Sat, 23 Apr 2005 02:21:33 -0400
Subject: [Microscopy] qualification of M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to add my two cents as someone who has had to use my CV
recently and has kept it up to date over the years. I solved the problem of
this issue by separating my publications into three groups, Refereed,
Reviewed, and Non-Reviewed. My definitions are that if it goes to two or
more reviewers by an editor of a journal and I get back a referee report
that I may have to respond to if they are not satisfied, then it is a
peer-reviewed, refereed article. If the article gets reviewed by peer
organizers of a meeting as to its fitness, quality, and scientific value for
inclusion into a meeting's proceedings where there is a possibility for it
to be rejected if it is not up-to-standards, then it is a Reviewed paper.
An article that is written that is included in a published periodical where
it may only be reviewed by an editor for content is a non-reviewed article.
I have all of my M&M abstracts listed under Reviewed since I know the
procedures for reviewing the abstracts at the Program Planning Meeting (as
Nestor, Bob, and Dave have mentioned in their posts). The few articles that
I have had in "Microscopy Today" are listed as non-reviewed, even though I
know that Ron Anderson critically reviews all of the MT articles before he
includes them.

By choosing to list my publications in this manner, it leaves no doubts for
someone reviewing my CV as to how the papers should be weighted.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127


-----Original Message-----
} From: Marek Malecki, M.D., Ph.D. [mailto:mmalecki-at-wisc.edu]
Sent: Thursday, April 21, 2005 7:16 AM
To: microscopy-at-microscopy.com

Greetings, All!
I have a dispute with UW administration over the number of publications
generated / year. I would appreciate seeing opinions of people , who went
through similar hurdles with their sponsors.
In particular, are M&M contributions considered peer-reviewed articles or
not? They are longer than 100 words-long ACB, FASEB, SMI abstracts and
longer than many rapid communications. They are also supposed to be
peer-reviewed. The data in them are distilled to the very core of the
communication.

Perhaps posting all Proceedings in complete forms on the Internet would
boost their prestige / recognition?

Cheers,
Marek






From MicroscopyL-request-at-ns.microscopy.com Sat Apr 23 02:02:50 2005



From: Alberto Diaspro :      diaspro-at-fisica.unige.it
Date: Sat, 23 Apr 2005 09:16:35 +0200
Subject: [Microscopy] Fluorescence School in Genoa, Italy - 13-15 September 2005, 1st announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

PLEASE, PASS THIS ANNOUNCEMENT.

Dear friends,
this year Fluorescence School will be held in Genoa on 13-15 September
2005. You can find news and information on
http://www.fluorescence-foundation.org/agenda.htm

Since the number of partecipants is limited, book your site now!

All my best
Alby



------------------------------------------------------------------------
------------------------------------------------------------------------
------------------------------------------------
[...] Dance with wolves and count the stars, including the
unseen...(L.Ferlinghetti, Challenges to young poet, 2001)

------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------
Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department
of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309; URL:
http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html

------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Sat Apr 23 08:30:32 2005



From: bdrysdale-at-fibrolight.com (by way of MicroscopyListserver)
Date: Sat, 23 Apr 2005 08:52:34 -0500
Subject: [Microscopy] viaWWW: Thanks / Rife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bdrysdale-at-fibrolight.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, April 22, 2005 at 14:35:16
---------------------------------------------------------------------------

Email: bdrysdale-at-fibrolight.com
Name: Robert Drysdale

Organization: Fibrolight Technology Inc

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Greetings

Thanks Geoff & Paul for your kind words.
I simply enjoy the study of Microscopy and it's
fascinating History. Mr/Dr Rife and his credentials are of no concern to me, just the History.

"Life is short,the Art is long"

Regards
R Drysdale BSc
Fibrolight Technology Inc

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 23 10:52:03 2005



From: Ivan Petrov :      petrov-at-mrl.uiuc.edu
Date: Sat, 23 Apr 2005 11:12:55 -0500
Subject: [Microscopy] RE: RE: Xenosput coater with Fe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Scott and Colin,



You can sputter magnetic materials with a magnetron in two ways, at least.



One is to use a very thin foil as a target - several hundred microns and stronger magnets. The magnetic field lines will penetrate the thin foil and the magnetron discharge will ignite. You have to change the foil quite often as it will sputter away fast.



If you have a target several mm thick, you can make a grove with approximately square cross-section (say 1x1mm) in the middle of the race track that you see on your Cr target.

That profile will form a ExB configuration in the grove and you will get a magnetron discharge. The edges of the grove erode pretty rapidly and the deposition rate will change with time but the target will last longer. This is just between you and me since it is an unpublished secret of mine J

Best

Ivan



-----Original Message-----
From: Scott Walck on Comcast [mailto:swalck-at-comcast.net]
Sent: Sat 4/23/2005 00:52
To: MicroscopyListserver
Cc: Colin.Veitch-at-csiro.au
Subject: [Microscopy] RE: Xenosput coater with Fe





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Colin, Not being familiar with the Xenosput system, I looked it up to see
whether it was a magnetron sputter system. Yep, it was. If you have a
ferromagnetic material, it will not sputter because it screws up (And that
is a technical phrase!) the magnetic field lines that guides the ions to the
target for sputtering. It is the presence of the magnetic lines that gives
you the little racetrack patterns that appear on targets when you use them.
You can get a thin Fe film by ion sputtering with no problems. Just like
the Cr target, you should sputter away the oxide coating while shielding
your sample prior to actually coating.

Disclaimer:
South Bay Technology manufactures and sells the IBS/e ion beam sputter unit
for applying uniform thin coatings on samples and substrates by ion
sputtering. If you visit our website, www.southbaytech.com, you can find
our representative in Australia.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127

-----Original Message-----
} From: Colin.Veitch-at-csiro.au [mailto:Colin.Veitch-at-csiro.au]
Sent: Thursday, April 21, 2005 2:18 AM
To: Microscopy-at-msa.microscopy.com
Subject: [Microscopy] Xenosput coater with Fe




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---

Hi All,

We have a Xenosput coater which we use to deposit Cr films on samples
for examination in our FESEM. One of my colleagues wants to deposit
some very thin Fe films using the Xenosput. I have had a steel target
made up and replaced the chromium one with it but can't seem to get an
arc to strike.

I'm wondering if it won't work with a magnetic material. (After my
attempts I replaced the Cr target and everything was fine, so I don't
think it is the Xenosput itself.) Has anyone ever tried using a steel
target in one of these things, and if so did they have any success? An
option is to try a stainless steel target and see how that goes is
suppose.

Any help with this would be appreciated.

Cheers.

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.











From MicroscopyL-request-at-ns.microscopy.com Sat Apr 23 14:42:03 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 23 Apr 2005 13:03:49 -0700
Subject: [Microscopy] EBSD on copper damascene runners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers:

I'm seeking feedback on eBSD analysis of as-fabricated
copper damascene IC runners. During Scanning 2005, I
gave a presentation about this and recrystalization of
the top level of a six layer IC. What is puzzling is
the lack of {111} orientation preference in the electrodep
layer versus that of the seed layer. If the seed layer
is essentially 95% {111} but the electrodep layer is
seemingly uniform in orientation, what does this mean
about the electrodep layer?

I see twinning (mostly Sigma 3) in the seed layer but hardly
any in the electrodep layers. The IC I'm currently studying
is a Motorola G4 PPC. The EBSD was done at 25C and 150C
with 150C for up to 117 hours.

Any idea what is going on here? Why doesn't the electrodep
layers exhibit the {111} preference of the seed layer?
What does this suggest about metal reliability and electromigration?
The runners range from 3.35u to .2u wide. Radically different
H/W ratios.

All feedback appreciated.

gary g.



From MicroscopyL-request-at-ns.microscopy.com Sat Apr 23 16:45:55 2005



From: :      Colin.Veitch-at-csiro.au
Date: Sun, 24 Apr 2005 08:07:29 +1000
Subject: [Microscopy] Thank you for Xenosput info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

Thank you very much for all of your responses regarding the use of a Fe target in a Xenosput (a magnetron sputter system).

It looks like we'll start off with a non-magnetic stainless steel first and then progress to a thin Fe target later. As the purity of the sputtered film at this stage is not critical (hence using mild steel first) the other components of the target won't matter too much.

Cheers and thanks again.

Colin Veitch

CSIRO TFT Geelong.



From MicroscopyL-request-at-ns.microscopy.com Sun Apr 24 09:02:03 2005



From: Lou Ross :      RossLM-at-missouri.edu
Date: Mon, 25 Apr 2005 09:10:33 -0500
Subject: [Microscopy] Re: viaWWW: microtome polypropylene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think that in reality one has to accept the fact that these articles would
be classified as "Peer-reviewed conference proceedings" which do not have
the same weight as longer articles. This is despite the fact that it takes
really a lot of effort to say everything in a very compact, 2 page format,
which is next thoroughly reviewed.
The South African National Research Foundation would classify them just as I
said, and I believe this is correct. Unfortunately!

Regards

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dr Wojciech J. Przybylowicz
Materials Research Group
iThemba LABS
PO Box 722
Somerset West 7129 South Africa
E-mail: przybylowicz-at-tlabs.ac.za
Fax: +27-21-8433543
Phone: +27-21-8431166 (direct); 8431000 (reception)
Cell: +27-82-563 7925
http://www.tlabs.ac.za
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx


----- Original Message -----
} From: "Scott Walck on Comcast" {swalck-at-comcast.net}
To: "MicroscopyListserver" {Microscopy-at-microscopy.com}
Sent: Saturday, April 23, 2005 8:21 AM

Although this might not help in cutting polypropylene across the
surface, I thought I'd mention a tip I learned last week for make
cross-sectioning. Dip the polypropylene in IPA, used as a wetting
agent, then into water, then into LN2, then snap it. I have yet to
try this, but I am told that the cross-sections turned out extremely
well for SEM.

Lou Ross


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 25 08:53:44 2005



From: Lou Ross :      RossLM-at-missouri.edu
Date: Mon, 25 Apr 2005 09:17:57 -0500
Subject: [Microscopy] 3rd annual Short Course on Computer-Assisted Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Second Reminder: There are still a few more openings for this short
course and workshop. Registration deadline is May 4.

The Electron Microscopy Core Facility at the University of
Missouri-Columbia is hosting the 3rd annual Short Course and Workshop
on Computer-Assisted Image Analysis and Measurement taught by Dr.
John C. Russ on May 25-27, 2005. This popular course is intended to
familiarize users of image analysis equipment with the fundamental
principles and methods available to obtain meaningful results, and to
educate laboratory supervisors or research professionals seeking to
learn how to use such methods in their applications. The techniques
are applicable to fields ranging from materials, geological and
biological/medical research to food technology and manufacturing
quality control.

The course relies heavily on tightly coupled lectures and hands-on
experience with the various techniques. The laboratory includes a
wide variety of image analysis methods designed to cover the range of
approaches and tools, and a detailed set of practical instructions to
enable their use with a minimal learning curve. No specific
background is assumed, although users should already be familiar with
microscopy or other imaging technology, and the techniques required
to obtain the images to be measured. Many of the examples used in the
course involve light or electron microscope images, but students are
invited to bring their own most interesting images (TIFF files) for
discussion and analysis.

Image analysis and measurement methods are used in a broad range of
applications and are usually concerned with extracting a few
numerical values, such as the number, size, shape or location of
objects from the image. In other cases, global structural parameters
such as measures of the volume and surface of structures present are
of interest. These measurements may require image processing to
correct defects, feature enhancement, comparison of multiple images,
object recognition, or other steps. Ultimately, the image is reduced
to just the features of interest. Measurements on these individual
features, or on the image as a whole, must then be obtained and
interpreted in a proper stereological context to obtain useful data
about the objects. Statistical interpretation of the data allows
comparisons of different populations, understanding of distribution
plots, and other inferences about the original objects. Structural
modeling and geometric probability can be used to develop models for
this interpretation.

Dr. Russ is the internationally acclaimed author of innumerable
articles and several books on image analysis techniques and digital
imaging, including the The Image Processing Handbook. He is widely
known for his workshops and short courses and has helped to develop
software packages to make image analysis methods more accessible to
non-specialists.

The registration fee is $1100 and has an enrollment limit of 20. More
information can be found at
{http://www.emc.missouri.edu/works.htm} http://www.emc.missouri.edu/works.htm,
or by contacting course coordinator Lou Ross at 573.882.4777 or
rosslm-at-missouri.edu.

Lou Ross
--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu

--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=5414
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu


From MicroscopyL-request-at-ns.microscopy.com Mon Apr 25 14:52:01 2005



From: Robert H. Olley :      hinmeigeng-at-hotmail.com
Date: Mon, 25 Apr 2005 20:15:41 +0000
Subject: [Microscopy] Micotoming polypropylene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Judy,

I've done lots of microtoming of PP: normally I've mounted the specimen
directly on a dry ice bucket using freezable acrylic compounds with a names
such as Tryco-M-Bed or Cryo-M-Bed. These are obtainable from your friendly
local microscopy supplier. After cutting the material can be washed from
the sections or stub with water.

I'd like to ask, are you trying to prepare sections for polarizing optical
microscopy, or surfaces for electron microscopy? I've had years of work
with PP using POM, SEM and TEM - if you want further details feel free to
ask.

For polypropylene TEM morphology you might like to look at:

http://www.personal.rdg.ac.uk/~spsolley/Picture_Gallery/new_pgal.html

and click on the tabs "Impact Polypropylene" and "Row Structures"

-----------------------------------
Robert H. Olley
Physics Department
University of Reading, England
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------




From MicroscopyL-request-at-ns.microscopy.com Mon Apr 25 18:58:38 2005



From: Chaoying Ni :      cni-at-UDel.Edu
Date: Mon, 25 Apr 2005 20:22:56 -0400 (EDT)
Subject: [Microscopy] Re: EBSD on copper damascene runners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Cu seeds are usually sputter coated. Any sputter coated layers tend to
have textures such as {111} for fcc. Cu electrodep on wafers is done
violently (mechanical rotating) in a very short period of time (mins).
Those pre-formed seeds may actually act as a buffer layer rather than the
nuclei from which columnar {111} Cu would grow. What's more, post-annealing
is a standard procedure, which would trigger recrystalization to kill the
texture should that pre-exist. Equiaxed grains are beneficial to both
chip process and interconnect conductivity.

Twinning may happen during process, deformation, or recrystalization.
The actual cause for triggering such a transformation is a million dollar
question. Sputtered Cu on wafer always has a lot of twinning.

My depreciated $0.02

-cni
****************************************
The W.M. Keck Electron Microscope Facility
Facility location: 022 Spencer Laboratory
Mailing address:
201 duPont Hall
Dept of Matls Sci & Eng
University of Delaware
Newark, DE 19716
(302) 831-2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************


On Sat, 23 Apr 2005, Gary Gaugler wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi Listers:
}
} I'm seeking feedback on eBSD analysis of as-fabricated
} copper damascene IC runners. During Scanning 2005, I
} gave a presentation about this and recrystalization of
} the top level of a six layer IC. What is puzzling is
} the lack of {111} orientation preference in the electrodep
} layer versus that of the seed layer. If the seed layer
} is essentially 95% {111} but the electrodep layer is
} seemingly uniform in orientation, what does this mean
} about the electrodep layer?
}
} I see twinning (mostly Sigma 3) in the seed layer but hardly
} any in the electrodep layers. The IC I'm currently studying
} is a Motorola G4 PPC. The EBSD was done at 25C and 150C
} with 150C for up to 117 hours.
}
} Any idea what is going on here? Why doesn't the electrodep
} layers exhibit the {111} preference of the seed layer?
} What does this suggest about metal reliability and electromigration?
} The runners range from 3.35u to .2u wide. Radically different
} H/W ratios.
}
} All feedback appreciated.
}
} gary g.
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Tue Apr 26 04:50:11 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Tue, 26 Apr 2005 07:44:26 -0230
Subject: [Microscopy] Objectives & cover glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

While evaluating microscope objectives for purchase, I note that objectives
can be degignated for "no cover glass", and objectives designed for both,
i.e., "with" and "without". What is the practical difference between these
2 types for photomicrography?

Our own applications will be 95% polished rock thinsections, which can be
divided equally between incident and transmitted polarized illumination.

tia & cheerios ... shAf :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Tue Apr 26 07:57:29 2005



From: Goheen, Michael P. :      mgoheen-at-iupui.edu
Date: Tue, 26 Apr 2005 08:21:57 -0500
Subject: [Microscopy] FW: Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Michael P. Goheen
Electron Microscopy Lab
Dept. of Pathology & Lab Medicine
Indiana University School of Medicine
Tel. 317-274-7604
Fax 317-274-5346
mgoheen-at-iupui.edu


Department of Pathology and Laboratory Medicine/ Indiana University School of Medicine
 
This is an announcement for an employment opportunity for an experienced Electron Microscopy Technologist (TE09) in Anatomic Pathology's clinical EM laboratory at Indiana University Medical Center in Indianapolis, Indiana.
 
 Minimum requirements and responsibilities:
 
*     BA/BS degree in one of the life sciences
 
*     Three years experience with transmission electron microscopy (TEM), preferably clinical
 
*     Proficiency in all phases of routine biological TEM
 
*     Experience with both digital and traditional photographic methodologies
 
*     Microscopy Society of America certification is highly preferred
 
*     Instruct new users on the operation of the transmission electron microscopes and ancillary equipment
 
*     Communicate effectively with internal and external inquiries related to the EM laboratory using both written and oral communication skills
 
*     Duties and responsibilities will include processing, cutting, staining, TEM examination and photography of pathologic specimens including kidney, muscle, nerve, cilia and tumors
 
A complete Job description along with benefits information is available from the IUPUI Human Resources department.  Salary is commensurate with experience.
 
Send your resume including a history of your clinical TEM experience and the names and contact information of 3 references to:
 
Susan Hill
Department of Pathology & Laboratory Medicine
Barnhill Drive
Medical Science Bldg, room A128
Indianapolis, IN 46202
suehill-at-iupui.edu
 
 
In the spring of 2006, the EM laboratory will move into a new state-of-the-art 65 million dollar laboratory building in downtown Indianapolis. As part of this relocation, along with the moving of our FEI CM 10, the lab is acquiring a new TEM equipped with a digital camera system. The lab provides services to Clarian Health partners (Methodist, Indiana University and Riley Hospitals) and clients throughout the United States.
 
 
 
 
                 
 
 
 



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 07:22:48 2005



From: pmccurdy-at-lamar.colostate.edu (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 07:47:43 -0500
Subject: [Microscopy] viaWWW: EDS Accuracy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pmccurdy-at-lamar.colostate.edu) from http://www.msa.microscopy.com/MLFormMail.html on Monday, April 25, 2005 at 14:06:09
---------------------------------------------------------------------------

Email: pmccurdy-at-lamar.colostate.edu
Name: Pat McCurdy

Organization: Colorado State University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: To Whom It May Concern:

What accuracy should I expect from my EDS system when analyzing non-conducting samples? I have consistently been getting a ratio of 1:2.5 for Si to O when analyzing a well-grounded piece of quartz. The applications guy for our system told me even if I coat the outside I will still get charging in the bulk which will skew my results. I tried to take into account the charging by looking at where the bremsstrahlung tailed off and adjusting the accelerating voltage by an appropriate amount. Still the results show an oxygen-to-silicon ratio greater than two. Any help would be greatly appreciated.

Sincerely,

Pat McCurdy
Research Scientist
Colorado State University


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 07:23:47 2005



From: jsnell-at-microbonds.com (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 07:48:43 -0500
Subject: [Microscopy] viaWWW: Leo/Zeiss 982 Service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jsnell-at-microbonds.com) from http://www.msa.microscopy.com/MLFormMail.html on Monday, April 25, 2005 at 14:06:28
---------------------------------------------------------------------------

Email: jsnell-at-microbonds.com
Name: James Snell

Organization: Microbonds, Inc.

Title-Subject: [Microscopy] [Filtered] Leo/Zeiss 982 Service

Question: We have a Leo DSM982 feSEM which we purchased USED. It has been a real problem machine and has not been successfully installed yet. There are very few reps available to service this machine at present and we have an urgent need.

The Zeiss reps available are quite good, but do not have enough available time to give us priority service until it is running.

Does anyone know of any other source than the manufacturer for servicing these SEMs (independent, recently retired)? It would be greatly appreciated.

James Snell
(905) 620-0623 x239

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 07:22:20 2005



From: mingzong-at-ualberta.ca (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 07:46:56 -0500
Subject: [Microscopy] viaWWW: TEM sample preparation of PE film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mingzong-at-ualberta.ca) from http://www.msa.microscopy.com/MLFormMail.html on Monday, April 25, 2005 at 11:41:11
---------------------------------------------------------------------------

Email: mingzong-at-ualberta.ca
Name: Mingzong

Organization: university of alberta

Title-Subject: [Microscopy] [Filtered] MListserver: TEM sample preparation of PE film

Question: Hi, I have questions of how to prepare polyethylene film thin sections used for TEM.
1. what kind of emdedding materials should I use for PE film? I tried spur, it seems that they can't adhere tightly, when I do the microtome, teh film is easily peel off from the embedded materil.
2. Do I have to use cryo-microtoming?
3. How to stain the samples? I found that in the literature, some one stained the thin sections while others stained the trimmed face off before microtoming? Which one is better? For each case, how long is approperate for the stainning?
Any suggestions is highly appreciated.


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 07:24:20 2005



From: dwberry-at-vt.edu (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 07:49:03 -0500
Subject: [Microscopy] viaWWW: Technics sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dwberry-at-vt.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, April 26, 2005 at 08:15:22
---------------------------------------------------------------------------

Email: dwberry-at-vt.edu
Name: David Berry

Organization: Materials Science and Engineering at Virginia Tech

Title-Subject: [Microscopy] [Filtered] (SEM sample prep) Technics sputter coater

Question: Hello All,

My department has acquired a used Technics Hummer II sputter coater for SEM sample prep. There is no documentation with it and I am unsure of its operation. We currently use a Denton Vacuum, DESK II, for all our sputtering needs and the set-up is a little different than the Technics system. Any information on this system would be extremely helpful. It would be really nice to have a manual if one still exists.

Thank you,

David Berry
Virginia Tech MSE Department



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 07:24:27 2005



From: Alicia.Roh-at-carolinashealthcare.org (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 07:49:35 -0500
Subject: [Microscopy] viaWWW: Lynx Automatic Tissue Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Alicia.Roh-at-carolinashealthcare.org) from http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April 26, 2005 at 11:26:56
---------------------------------------------------------------------------

Email: Alicia.Roh-at-carolinashealthcare.org
Name: Alicia Roh

Organization: Carolinas Medical Center

Title-Subject: [Microscopy] [Filtered] Lynx Automatic Tissue Processor

Question: We have recently purchased a Lynx el Automatic Tissue Processor from EMS to help
assist with our clinical tissue processing. It appears that this machine has
been utilized in other laboratories for a number of years, and we would like to
know if anyone has any successes/challenges they would like to share with us.
Currently we use an 8-hour protocol for soft tissue (prior to embedding in 100%
resin). Our main inquires at this moment are:

1) Do any of the hand-processed protocol times need to be adjusted for use with
the automatic processor?

2) Is Osmium fixation recommended with the machine, or should that be hand
processed separately?

3) Is there excessive evaporation of 100% ethanol and/or propylene oxide that we
would have to account for by increasing volume?

4) Any challenges with the resin polymerizing in the vials, or excessive
dripping between vial rotations?

5) Does anyone use optical lens paper to 'wrap' the specimens prior to insertion
into the baskets. (Sometimes our samples are small enough to fall through the
holes).

Any advice would greatly be appreciated! Thanks!


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 09:42:54 2005



From: gary.m.brown-at-exxonmobil.com
Date: Wed, 27 Apr 2005 10:06:37 -0500
Subject: [Microscopy] Re: viaWWW: TEM sample preparation of PE film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

"The statements and opinions expressed here by Gary M. Brown represent
neither those of ExxonMobil Corporation nor its affiliates."

Mingzong,

I strongly recommend the following approach:
Cryo-face the sample using a glass or diamond knife in a
cryo-ultramicrotome.
Stain faced sample in RuO4 vapors.
Cut { 100 nm-thick sections from the stained face using a diamond knife
and ultramicrotome at ambient temperature.
Have fun in the TEM.

This procedure, as well as a one for preparation of samples for low voltage
SEM analysis of domain morphology of blends, is well documented in the
paper referenced below. Our lab uses it exclusively, over other techniques
for polyethylene, polypropylene, their blends and copolymers, with
excellent results. Detailed instructions can be found in the appendix.

The reference is: G. M. Brown and J. H. Butler, “New method for the
characterization of domain morphology of polymer blends using ruthenium
tetroxide staining and low voltage scanning electron microscopy (LVSEM)â€,
Polymer 38 (15), 3937 (1997).

Feel free to contact me off-line with questions regarding the method.

Cheers,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Technology & Engineering - West
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: 281 834 2387
fax: 281 834 2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



mingzong-at-ualbe
rta.ca (by way
of To
MicroscopyList microscopy-at-microscopy.com
server) cc

Subject
viaWWW: TEM sample
04/27/05 07:46 preparation of PE film
AM











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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mingzong-at-ualberta.ca) from
http://www.msa.microscopy.com/MLFormMail.html on Monday, April 25, 2005 at
11:41:11
---------------------------------------------------------------------------

Email: mingzong-at-ualberta.ca
Name: Mingzong

Organization: university of alberta

Title-Subject: [Microscopy] [Filtered] MListserver: TEM sample preparation
of PE film

Question: Hi, I have questions of how to prepare polyethylene film thin
sections used for TEM.
1. what kind of emdedding materials should I use for PE film? I tried spur,
it seems that they can't adhere tightly, when I do the microtome, teh film
is easily peel off from the embedded materil.
2. Do I have to use cryo-microtoming?
3. How to stain the samples? I found that in the literature, some one
stained the thin sections while others stained the trimmed face off before
microtoming? Which one is better? For each case, how long is approperate
for the stainning?
Any suggestions is highly appreciated.


---------------------------------------------------------------------------




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 11:23:15 2005



From: Edward Calomeni :      calomeni-1-at-medctr.osu.edu
Date: Wed, 27 Apr 2005 12:46:44 -0400
Subject: [Microscopy] Re: Lynx Automatic Tissue Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Alicia ,


I use RMC's processor, not Leica, but here are my answers to your
question. I believe there should be minor (or no) differences between
the two processors.


1) Do any of the hand-processed protocol times need to be adjusted for
use with
the automatic processor?

My only change here is to shorten the infiltration time. I use 7 hours
verses overnight by hand.


2) Is Osmium fixation recommended with the machine, or should that be
hand
processed separately?

I process OsO4 in the processor. Same time as by hand (1 hour).


3) Is there excessive evaporation of 100% ethanol and/or propylene
oxide that we
would have to account for by increasing volume?

This answer will have to come from a Leica user, if there is, it should
be very minimal.


4) Any challenges with the resin polymerizing in the vials, or
excessive
dripping between vial rotations?

If I set the processor up for a run over the weekend, I do not use 100%
resin in the last vial. It will polymerize almost completely and be
very hard to retrieve the tissue. I use two 1:1 resin:acetone vials.


5) Does anyone use optical lens paper to 'wrap' the specimens prior to
insertion
into the baskets. (Sometimes our samples are small enough to fall
through the
holes).

I use a product called Histowrap (from Obex Industries). There are
very few tissues I do not wrap. Try to leave the tissue exposed
(wrapped) with only one layer of wrap.

Any advice would greatly be appreciated! Thanks!


Automatic tissue processors will not cut down on turn-around-time, but
they do make life easier.

Best of luck,

Ed

---------------------------------------------------------------------------



Edward Calomeni
Director EM Lab
Ohio State University - Pathology
M018 Starling Loving Hall
320 W. 10th Ave.
Columbus, OH 43210-1240
614-293-5580 (office)
614-293-8806 (lab)
calomeni-1-at-medctr.osu.edu



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 15:28:32 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 28 Apr 2005 08:53:03 +1200
Subject: [Microscopy] Re: viaWWW: EDS Accuracy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I suspect that your experience illustrates more the problems with quantitative oxygen
analysis (by EDS) than with the analysis of nonconducting samples.

EDS can and does give very good quantitative analytical results, for elements from
sodium up, for all sorts of silicates, most if not all of which are nonconducting.

What oxygen standard(s) are you using?

cheers

rtch


}
} Email: pmccurdy-at-lamar.colostate.edu
} Name: Pat McCurdy
}
} Organization: Colorado State University
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: To Whom It May Concern:
}
} What accuracy should I expect from my EDS system when analyzing
} non-conducting samples? I have consistently been getting a ratio of
} 1:2.5 for Si to O when analyzing a well-grounded piece of quartz. The
} applications guy for our system told me even if I coat the outside I
} will still get charging in the bulk which will skew my results. I
} tried to take into account the charging by looking at where the
} bremsstrahlung tailed off and adjusting the accelerating voltage by an
} appropriate amount. Still the results show an oxygen-to-silicon ratio
} greater than two. Any help would be greatly appreciated.
}
} Sincerely,
}
} Pat McCurdy
} Research Scientist
} Colorado State University
}
}
} ----------------------------------------------------------------------
} -----
}

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 16:49:11 2005



From: monica.iliescu-at-polymtl.ca (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 17:14:18 -0500
Subject: [Microscopy] viaWWW: SEM, samples of blood clots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (monica.iliescu-at-polymtl.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, April 27, 2005 at 07:51:45
---------------------------------------------------------------------------

Email: monica.iliescu-at-polymtl.ca
Name: Monica ILIESCU

Organization: Ecole Polytechnique

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello all:

I would like to learn if someone of you imagined, in conventional high vacuum SEM, samples of blood clots, and if yes, how proceed for the specimen preparation.

Thank you and best greetings,

Monica

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 16:49:43 2005



From: rpowell-at-nanoprobes.com (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 17:14:50 -0500
Subject: [Microscopy] viaWWW: Job Opening Microscopy Technician, Nanoprobes Inc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rpowell-at-nanoprobes.com) from on Wednesday, April 27, 2005 at 14:04:44
---------------------------------------------------------------------------

Email: rpowell-at-nanoprobes.com
Name: Rick Powell

Organization: Nanoprobes, Inc

Title-Subject: [Microscopy] [Filtered] Vacancy: Microscopy Technician, Nanoprobes Inc.

Question: Nanoprobes, Incorporated is a developer and manufacturer of immunogold probes and reagents located on Long Island, NY.

We are looking for a MICROSCOPY TECHNICIAN (recent Associates or BS). We need someone to help us keep our TEM up and running, then use TEM, light and fluorescence microscopy to evaluate prototypes and new products.

Job functions:

(1) Maintain and operate our TEM, schedule and coordinate repairs, maintain and manage ancillary facillities - darkroom, processing equipment and chemicals, and film.

(2) Transmission electron microscopy of samples including gold and other metal nanoparticles, autometallographically enhanced gold, and biological specimens stained or labeled with these reagents; negative staining and counterstaining as required.

(3) Help us acquire and set up lab and equipment for biological specimen processing (embedding, sectioning, etc.), then apply these methods to develop systems in which to test new staining and immunolabeling reagents.

(4) Light microscopy and fluorescent microscopy, including correlative light/electron and fluorescence/electron microscopy.

You might also work with some other biological immunostaining and detection applications (blots, gels), depending on the workload for microscopy.

We are looking for someone who can develop standard procedures for microscope operation, and for staining procedures for use as test systems to evaluate new reagents. The successful applicant will also help us implement systems for archiving and sharing microscopic data and images.

If this is you, please fax your resume to (631) 980-3608, or e-mail rpowell-at-nanoprobes.com. Unfortunately we can't offer relocation, so local candidates will be preferred.

*************************************************************
NANOPROBES, Incorporated * www.nanoprobes.com
95 Horse Block Road, Yaphank, NY 11980-9710, USA
*************************************************************

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 16:50:12 2005



From: shaenon-at-hotmail.com (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 17:15:18 -0500
Subject: [Microscopy] viaWWW: softening insect cuticle for histological work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (shaenon-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, April 27, 2005 at 14:50:27
---------------------------------------------------------------------------

Email: shaenon-at-hotmail.com
Name: Shannon Mahony

Organization: Carleton University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi, I was wondering if anyone has any suggestions for softening insect cuticle for histological work. I am having much difficulty sectioning insect ears as the cuticle is so hard. Any ideas or suggestion?

Thanks in advance!

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 17:47:34 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 27 Apr 2005 19:11:09 -0500
Subject: [Microscopy] Re: Objectives & cover glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Pat,
The quantification of the EDS results on an SEM is complicated and very
dependant on many factors of the SEM, EDS system and sample. The
quantification of the very light elements is further complicated by the very
soft nature of the x-rays, which means that not all of them are detected,
and the very large correction factors that are calculated for atomic number
and absorption for the elements below sodium on the periodic table. If your
sample charges, then the apparent electron beam voltage drops as the sample
builds up a negative charge, which changes the calculation of the correction
factors. As a final problem, if the EDS detector gets contaminated with a
film of oil from the SEM pumping system, the softer x-rays from the lighter
elements get preferentially absorbed. I used to go from an oxygen peak half
the height of the silicon on my SiO2 standard, when the window was dirty, to
an oxygen peak twice the height of the Si, after I had cleaned the window.
Some questions: What is your EDS take-off angle? What is your EDS window
material? Is your SiO2 sample polished flat and exactly perpendicular to the
beam. Is it coated with a thin layer of carbon to prevent charging or are
you using variable pressure? Is your EDS window clean or can it be cleaned?
Sometimes it is better to use your sample as a standard in the EDS system,
than to try to get the EDS to produce the right numbers (standardless) for
these materials containing very light elements. In answer to your question,
not much accuracy.
Good luck,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "by way of MicroscopyListserver" {pmccurdy-at-lamar.colostate.edu}
To: {microscopy-at-microscopy.com}
Sent: Wednesday, April 27, 2005 5:47 AM

Hi, Michael

Coverslips are part of the optical train. If the system calls for "no
coverslip" and you use one, you will get spherical abberrations (lack of
focus, hazy image).

For your transmitted light work, I would recommend using a coverslip
(#1-1/2). For your reflected light work, no coverslip. The objectives you
use can also be y our guide.

Hope this is helpful.

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to
learn more about "Optimizing LIght Microscopy". Copies still available
through MME... even for class-room lots ... and we give quantity discounts.


At 05:14 AM 4/26/2005, michael shaffer wrote:


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From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 20:26:03 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 27 Apr 2005 20:49:51 -0500
Subject: [Microscopy] Re: Re: viaWWW: EDS Accuracy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Along these lines, I was recently asked if you could get an idea about
stratification of different elements in a sample using EDS by adjusting the
kV so that the beam would penetrate to different depths and then comparing
the resulting spectra. The investigator expects that when a particular
material (primarily light elements with some Zn and Mn of interest) dries
down, some of the components will settle at different rates based on
particle size and composition. He would be content with some very general
data that would confirm or reject his theory.

Is this possible or reasonable to get the desired information? Would Monte
Carlo simulation be able to predict this type of information and help in
determining the necessary sample thickness to make the results meaningful?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

On 4/27/05 6:09 PM, "Mary Mager" {mager-at-interchange.ubc.ca} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Dear Pat,
} The quantification of the EDS results on an SEM is complicated and very
} dependant on many factors of the SEM, EDS system and sample. The
} quantification of the very light elements is further complicated by the very
} soft nature of the x-rays, which means that not all of them are detected,
} and the very large correction factors that are calculated for atomic number
} and absorption for the elements below sodium on the periodic table. If your
} sample charges, then the apparent electron beam voltage drops as the sample
} builds up a negative charge, which changes the calculation of the correction
} factors. As a final problem, if the EDS detector gets contaminated with a
} film of oil from the SEM pumping system, the softer x-rays from the lighter
} elements get preferentially absorbed. I used to go from an oxygen peak half
} the height of the silicon on my SiO2 standard, when the window was dirty, to
} an oxygen peak twice the height of the Si, after I had cleaned the window.
} Some questions: What is your EDS take-off angle? What is your EDS window
} material? Is your SiO2 sample polished flat and exactly perpendicular to the
} beam. Is it coated with a thin layer of carbon to prevent charging or are
} you using variable pressure? Is your EDS window clean or can it be cleaned?
} Sometimes it is better to use your sample as a standard in the EDS system,
} than to try to get the EDS to produce the right numbers (standardless) for
} these materials containing very light elements. In answer to your question,
} not much accuracy.
} Good luck,
} Mary Mager
} Electron Microscopist
} Department of Materials Engineering
} University of British Columbia
} 6350 Stores Road
} Vancouver, B.C. V6T 1Z4
} CANADA
} Tel: 604-822-5648
} Fax: 604-822-3619
} e-mail: mager-at-interchange.ubc.ca
} ----- Original Message -----
} } From: "by way of MicroscopyListserver" {pmccurdy-at-lamar.colostate.edu}
} To: {microscopy-at-microscopy.com}
} Sent: Wednesday, April 27, 2005 5:47 AM
} Subject: [Microscopy] viaWWW: EDS Accuracy
}
}
} }
} }
} } --------------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------------
} -----
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (pmccurdy-at-lamar.colostate.edu) from
} http://www.msa.microscopy.com/MLFormMail.html on Monday, April 25, 2005 at
} 14:06:09
} } --------------------------------------------------------------------------
} -
} }
} } Email: pmccurdy-at-lamar.colostate.edu
} } Name: Pat McCurdy
} }
} } Organization: Colorado State University
} }
} } Title-Subject: [Microscopy] [Filtered] MListserver:
} }
} } Question: To Whom It May Concern:
} }
} } What accuracy should I expect from my EDS system when analyzing
} non-conducting samples? I have consistently been getting a ratio of 1:2.5
} for Si to O when analyzing a well-grounded piece of quartz. The applications
} guy for our system told me even if I coat the outside I will still get
} charging in the bulk which will skew my results. I tried to take into
} account the charging by looking at where the bremsstrahlung tailed off and
} adjusting the accelerating voltage by an appropriate amount. Still the
} results show an oxygen-to-silicon ratio greater than two. Any help would be
} greatly appreciated.
} }
} } Sincerely,
} }
} } Pat McCurdy
} } Research Scientist
} } Colorado State University
} }
} }
} } --------------------------------------------------------------------------
} -
} }
}
}




From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 21:57:06 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 27 Apr 2005 20:21:25 -0700
Subject: [Microscopy] Re: viaWWW: EDS Accuracy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would expect to get very good results. No matter what.
Unfortunately, this does not always happen. Some times,
it is operator error...sigh.

What KV are you using? What probe size? What specimen
current?

For light elements, low KV is fine/best. For Si and O and lower
than Si, the K alpha shells are predominant. So I figure
that you only need 4-5KV. This is what I use (5KV). I coat
the specimen with around 50A of Au/Pd or Pt. No problem.

So, when done, EDAX Genesis will produce intensity error
ratios for each detected and ID element. If the ratio is higher
than about 12% or so, the quant is probably bad. Mostly this means to me
that I made an error in Z ID. If the Genesis HPD curve
says that the IDs are correct, but intensity ratios are bad,
then either I missed something really close in Z value or
there is indeed a specimen issue. Usually it is my fault.
The intensity ratios and HPD help to sort this out.

I use SiO2 for Si and O but also X-Checker Extra BN for N. So this I think
pretty much nails Si and Al. Then, I use C and N and F
to close in on the lighter elements around O. X-Checker Extra
goes down to Be. That is useful to check all elements from
Cu on down and a test for Mn (the FWHM test).

I've not had a quant problem with Genesis. Many of these
quants have been checked against other methods. I have seen no more
than about 2-3%% or so of deviation. No guess why the difference.
Perhaps it is volumetric interaction. And of course, which is
right and which is wrong?

Also, which quant method are you using for your specimen?
The default is ZAF. Fine. If you are using bulk specimens,
I think that RhoZAF is better. For best results, PhiRhoZAF
is IMO, better. But Genesis offers SEC correction factors
to tweak the ZAF values based on whatever standard you use
and admire.

Disclaimer: I use EDAX Genesis. I do not sell it, loan it
or lease it. I am just a happy user. I also do not sell
X-Checker.

gary g.


At 05:47 AM 4/27/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Wed Apr 27 23:44:35 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 28 Apr 2005 00:10:04 -0500
Subject: [Microscopy] Stratification Analysis via EDS by varying HT.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stratification Analysis vai EDS by varying HT.

Debbie

IMHO, this is an inadvisable approach and will be potentially
frought with problems and inaccuracies. I certainly would
only try this as an absolute last resort. There are much better
and more accurate approaches.

The simpliest would be for your user to make a cross-section of the sample,
(s)he can then image and analyze the respective strata by XEDS
using conventional approaches, geometries and correction factors.

Talk to a Materials Scientist/Metallurgist at Purdue's Materials
Engineering / Microstructural Analysis Facility. They should
be able to help you, as this is a routine procedure in materials
characterization.

Nestor
Your Friendly Neighborhood SysOp.




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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Thu Apr 28 01:22:58 2005



From: :      Colin.Veitch-at-csiro.au
Date: Thu, 28 Apr 2005 16:47:38 +1000
Subject: [Microscopy] Edwards E306 coating unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

I have just had to resurrect an old E306 coating unit which hasn't been
used for a while and the manuals seem to have disappeared. The valve
handle has an in and out position, with labels of, backing, roughing,
valves closed, glow discharge, fine pumping and open. Which apply to
the in and out position, and what should be the correct pumping
sequence?

Any help would greatly be appreciated.

Thank you very much.

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



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received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 28 02:51:25 2005



From: Gerd Leitinger :      gerd.leitinger-at-meduni-graz.at
Date: Thu, 28 Apr 2005 10:14:33 +0200
Subject: [Microscopy] Re: viaWWW: Lynx Automatic Tissue Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Alicia,

We have had a Lynx processor for a while, so I can answer some of
your questions:

1) We do not adjust the times compared to hand processing

2) We still do the osmium fixation step by hand - we were worried of
getting an osmium deposit in the machine

3) Ethanol is all right as there is a rubber cover that closes the vessels
most of the time, but propylene oxide does evaporate more quickly. I
can not tell you whether propylene oxide is all right in long protocols
since we work with acetone dehydration and do not use propylene
oxide. You could fill the vessels up and do a test run with propylene
oxide.

4) We have to clean all the vessels and the machine with acetone after
each run. We embed in TAAB resin and are able to do the first 2
changes of 100% TAAB in the machine as long as we keep the
incubation times low.

5) We do not use the Lynx for small or fragile specimens ( such as
single cells or Vibratome sections ); I found it was rougher on the tissue
than we are when we hand process it.

I hope this has helped

Gerd

Datum: Wed, 27 Apr 2005 07:49:35 -0500
An: microscopy-at-microscopy.com
Von: Alicia.Roh-at-carolinashealthcare.org (by way of
MicroscopyListserver)
Betreff: viaWWW: Lynx Automatic Tissue
Processor

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Alicia.Roh-at-carolinashealthcare.org) from
http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April
26, 2005 at 11:26:56
} ---------------------------------------------------------------------------
}
} Email: Alicia.Roh-at-carolinashealthcare.org
} Name: Alicia Roh
}
} Organization: Carolinas Medical Center
}
} Title-Subject: [Microscopy] [Filtered] Lynx Automatic Tissue
Processor
}
} Question: We have recently purchased a Lynx el Automatic Tissue
Processor from EMS to help
} assist with our clinical tissue processing. It appears that this machine
has
} been utilized in other laboratories for a number of years, and we
would like to
} know if anyone has any successes/challenges they would like to
share with us.
} Currently we use an 8-hour protocol for soft tissue (prior to
embedding in 100%
} resin). Our main inquires at this moment are:
}
} 1) Do any of the hand-processed protocol times need to be adjusted
for use with
} the automatic processor?
}
} 2) Is Osmium fixation recommended with the machine, or should that
be hand
} processed separately?
}
} 3) Is there excessive evaporation of 100% ethanol and/or propylene
oxide that we
} would have to account for by increasing volume?
}
} 4) Any challenges with the resin polymerizing in the vials, or
excessive
} dripping between vial rotations?
}
} 5) Does anyone use optical lens paper to 'wrap' the specimens prior
to insertion
} into the baskets. (Sometimes our samples are small enough to fall
through the
} holes).
}
} Any advice would greatly be appreciated! Thanks!
}
}
} ---------------------------------------------------------------------------
}




Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 28 04:18:30 2005



From: stuart McKernan :      stuartm-at-umn.edu
Date: Thu, 28 Apr 2005 04:43:17 -0500
Subject: [Microscopy] MMS Spring Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} The Minnesota Microscopy Society invites you to its Annual MMS Spring
} Symposium to be held on Friday May 6, 2005 at the Science Museum of
} Minnesota , 120 W. Kellogg Blvd., St. Paul in the Discovery Hall
} (www.sci.mus.mn.us).
}
} This year's focus is "Cutting Edge Technologies in Microscopy"
}
} Schedule
} 7:30 - 8:15 AM Registration, Continental Breakfast, and Vendor Displays
} 8:15 - 9:00 AM Tom Isabell, JEOL USA, Inc.
} Trends in Electron Microscopy, A Corrected View of the Future
} 9:00 - 9:45 AM David Larson, Imago Scientific Instruments;
} Analysis of Materials on an Atomic Scale
} 9:45 - 10:30 AM Break and Vendor Displays
} 10:30 - 11:15 PM Scott Chumbley, Iowa State University
} WebSEM: Interactive, On-Line Microscopy for Education
} 11:15 - 12:00 PM Scott Chumbley and Amy Chumbley - WebSEM Demo
} 12:00 - 1:00 PM Lunch and Vendor Displays
} 1:00 - 1:30 PM Business Meeting (Society elections, Project MICRO,
} etc.)
} 1:30 - 2:15 PM Paul Voyles, University of Wisconsin, Madison
} Imaging Single Impurity Atoms with Z-contrast STEM
} 2:15 - 3:00 PM Break and Vendor Displays
} 3:00 - 3:45 PM Duane Krueger, University of St. Thomas
} Windows into Fragile Materials: Confocal Light Microscopy and ESEM
}
} Registration
} The cost of the meeting will be $75 for MMS members and $85 for
} nonmembers. For students and K-12 teachers the registration fee is
} $35. This fee includes the meeting, buffet lunch, breakfast, coffee
} breaks, and a free pass to the Museum exhibits (a $7 value).
} Registrants can pay at the door, but reservations must be made in
} advance.
}
} The Science Museum of Minnesota always has an exciting array of
} exhibits. In addition, the Omnitheater features are "Kilimanjaro" and
} "Mars 3D". Tickets to the Omnitheater are extra.
}
} You must make your reservations by Tuesday, May 3rd, and you can do so
} by contacting Robert Lundquist (robltt-at-juno.com; 763-494-7945).
} Include your name, address, and phone number or e-mail address with
} your reservation. Due to the high cost to the Society, we will have to
} bill those who make reservations but do not show.

Prof. Stuart McKernan
IT Characterization Facility, University of Minnesota            
E-mail :  stuartm-at-umn.edu
12 Shepherd Labs,                                                    
                 Office:         (612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455                    
Lab:            (612) 626-7594






From MicroscopyL-request-at-ns.microscopy.com Thu Apr 28 08:36:50 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 28 Apr 2005 09:01:02 -0500
Subject: [Microscopy] viaWWW: softening insect cuticle for histological

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Believe it or not, I saw somebody successfully use Nair, the cosmetic
hair remover product, for this very purpose. As I recall it was a
project at the Southern Illinois University EM facility years ago
involving serial LM sections through the abdomens of flies
(affectionately referred to as the "bug butt" project).

For what it's worth.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu




-----Original Message-----
} From: by way of MicroscopyListserver [mailto:shaenon-at-hotmail.com]
Sent: Wednesday, April 27, 2005 5:15 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (shaenon-at-hotmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday,
April 27, 2005 at 14:50:27
------------------------------------------------------------------------
---

Email: shaenon-at-hotmail.com
Name: Shannon Mahony

Organization: Carleton University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi, I was wondering if anyone has any suggestions for
softening insect cuticle for histological work. I am having much
difficulty sectioning insect ears as the cuticle is so hard. Any ideas
or suggestion?

Thanks in advance!

------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 28 13:01:31 2005



From: David Kinast :      DKinast-at-Hitschfel.com
Date: Thu, 28 Apr 2005 13:28:17 -0500
Subject: [Microscopy] LM Job posting in St. Louis, MO

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hitschfel Instruments, Inc, the St. Louis based Olympus Microscope dealer
is seeking applicants for a microscope sales position in the St. Louis
Metro area. If you are interested and want to learn more or submit your
resume, please visit us by pasting this link into your browser:

hitschfel.com/employment.html

Thanks to all respondents and to Nestor for providing this valuable forum.



David L. Kinast
Hitschfel Instruments, Inc.
2333 South Hanley Rd.
St. Louis, MO 63144
Phone: 800/242-3501
Phone: 314/644-6660
Fax: 314/644-5877
dkinast-at-hitschfel.com
hitschfel.com




From MicroscopyL-request-at-ns.microscopy.com Thu Apr 28 14:25:43 2005



From: frank.karl-at-degussa.com
Date: Thu, 28 Apr 2005 15:50:10 -0400
Subject: [Microscopy] microscopist in search of flame AA method

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





This isn't microscopy, but...

I need recommendation for a procedure to determine the Al content in kaolin
clay by flame AA. Our "library" doesn't have any references and we are a
little isolated from the local university...

If anyone has a procedure they don't mind sharing or a citation to a
reference book please contact me.

Thanks!!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

frank.karl-at-degussa.com


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.



From MicroscopyL-request-at-ns.microscopy.com Thu Apr 28 14:31:46 2005



From: Gazda, Jerzy :      jerzy.gazda-at-ceriumlabs.com
Date: Thu, 28 Apr 2005 14:56:18 -0500
Subject: [Microscopy] Embeding PTFE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a need to embed for ultramicrotomy a non-woven PTFE fibrous pad
made out of nano-fibers. Spurrs and Epo-fix do not adhere well to the
materials and ultra-sections tend to split along epoxy-pad interface.
Any suggestions will be appreciated.

Thank you in advance.

Jerzy

******************************************************
Jerzy Gazda, Ph.D. CeriumLaboratories L.L.C.

Supervising Engineer 5204 E. Ben White
Blvd. - MS 512
Austin, TX
78741
TEL: 1-800-538-8450, Ext. 51453
jerzy.gazda-at-ceriumlabs.com
******************************************************






From MicroscopyL-request-at-ns.microscopy.com Thu Apr 28 15:03:01 2005



From: Beth Bray :      bbray-at-sc.rr.com
Date: Thu, 28 Apr 2005 19:18:35 -0400
Subject: [Microscopy] microscopist in search of flame AA method

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Colin

referring to the arrow/pointer end of the handle (opposite the bit you hold on to):


1
IN and fully CCW, arrow pointing to "VALVES CLOSED" is the off position, in which you
can switch off the rotary pump. For startup, turn on the rotary pump, let it run a minute
or so before turning the handle:

2
90 deg CW, handle pops out, arrow points to 'BACKING" is the standby position, you
can turn on the water, push the DIFF PUMP button, give the DP 30 mins or so to warm
up, load samples and fresh carbon rod while you're waiting, Pirani should reach about
10 to the -1, then:

3
Push the handle in, turn 180 deg CCW, arrow points to "ROUGHING", rotary pump
evacuates belljar while DP gets backed by ballast chamber, so don't spend too much
time in this phase in case ballast runs out of suck, just until Pirani gets to about 2 by 10
to the -1, then:

4
Turn handle CW 180 deg back to "ROUGHING", allow it to pop out, then turn a further
180 deg CW to the "OPEN" position (same position as ROUGHING but with handle out,
not in). This is the full evacuate mode, with the DP sucking on belljar and the RP
sucking on the DP, in which you can switch on the Penning gauge, and when the
vacuum is OK, you can turn on the power to the rods and do your coating.

I don't know about the Glow discharge position.

I can fax you some manual pages if you want.

cheers

rtch




Hi Frank, it has been a while since I did trace metals analysis, but these
links below are a good place to start. They are from the EPA's site for
SW-846 methods. I assume that you have all the particulars for setting up
the atomic absorption instrument. It is imperative that you suppress
aluminum's tendency to ionize by adding 1000 ppm potassium as KCl: see this
link http://www.epa.gov/epaoswer/hazwaste/test/pdfs/7000a.pdf , and read
section 3.1.4:

"Ionization interferences occur when the flame temperature is
sufficiently high to generate the removal of an electron from a
neutral atom, giving a positively charged ion. This type of interference can
generally be controlled by the addition, to both standard and sample
solutions, of a large excess (1,000 mg/L) of an easily ionized element such
as K, Na, Li or Cs."

Be sure to add the KCl to both samples and standards. You will also have to
make sure your aspiration "blank" is made up so that it has the same general
concentration of acid and KCl as your digestion blank, samples and standards
will have. A quick and dirty prep for the KCl solution is to keep adding
KCl to about 100 mL of deionized (or demineralized) water until no more will
go into solution. Then add 1 mL of it to each 100 mL of your working
standard or digested sample - add it before you make the solutions up to
final volume. Make a series of standards that will bracket the
concentration of your sample. You may have to dilute your unknown to get it
into the linear range for aluminum, and if that is the case, remember to add
another mL of your saturated KCl solution. I cannot remember the linear
range for aluminum in flame AA, but 30 mg/L sticks in my mind - though it
may be higher. At any rate, that should be in the manual that came with
your instrument.

Incidentally, you can use NaCl (table salt) instead of KCl, but you will
have to put up with the fiercely bright orange light caused by the sodium.
Not a pretty sight. Really tires the eyes!

This is a good first stop to look around:
http://www.epa.gov/epaoswer/hazwaste/test/main.htm

Go to this link and check it out for the exact method you want:
http://www.epa.gov/epaoswer/hazwaste/test/pdfs/chap3.pdf

For example, Method 3005 prepares ground water and surface water samples for
total recoverable and dissolved metal determinations by FLAA, ICP-AES, or
ICP-MS. The unfiltered or filtered sample is heated with dilute HCl and HNO
prior to metal determination.

Then there is Method 3050 which prepares waste samples for total recoverable
metals determinations by FLAA and ICP-AES, or GFAA and ICP-MS depending on
the options chosen. The samples are vigorously digested in nitric acid and
hydrogen peroxide followed by dilution with either nitric or hydrochloric
acid. The method is applicable to soils, sludges, and solid waste samples.

Then go to this link for Method 7020 for the specifics for aluminum by flame
AA: http://www.epa.gov/epaoswer/hazwaste/test/pdfs/7020.pdf

If you have any questions, please feel free to email me.

Regards,
Beth Bray
bbray-at-sc.rr.com




-----Original Message-----
} From: frank.karl-at-degussa.com [mailto:frank.karl-at-degussa.com]
Sent: Thursday, April 28, 2005 3:50 PM
To: microscopy-at-msa.microscopy.com





This isn't microscopy, but...

I need recommendation for a procedure to determine the Al content in kaolin
clay by flame AA. Our "library" doesn't have any references and we are a
little isolated from the local university...

If anyone has a procedure they don't mind sharing or a citation to a
reference book please contact me.

Thanks!!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

frank.karl-at-degussa.com


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender by
telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.





From MicroscopyL-request-at-ns.microscopy.com Thu Apr 28 19:01:44 2005



From: sghoshro-at-NMSU.Edu
Date: Thu, 28 Apr 2005 14:56:26 -0600 (MDT)
Subject: [Microscopy] leaf trichomes/teliospores

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everyone,

A colleague of mine is interested in removing trichomes and fungal
teliospores from grass leaf epidermis to do PCR. Is there any way she can
remove individual trichomes/teliospores while looking under a microscope
or there is some other way this can be achieved.

Any help will be highly appreciated.

Thanks,

Soumitra


*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 29 08:59:18 2005



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 29 Apr 2005 09:23:59 -0500
Subject: [Microscopy] Re: microscopist in search of flame AA method

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank, this question is in that category where I wonder what is the basic
question that leads you to attempt this particular solution. Of course, we
get a lot of questions like that on this list. We are asked how to do some
particular thing without much explanation of the ultimate goal or why the
particular technique has been chosen. I know that sometimes the poster is
not at liberty to say, but that doesn't stop me from wondering. Many times
another solution would seem to be more effective.

It seems Ms. Bray is offering a trace element solution, but aluminum in
kaolinite is anything but trace. I am not a clay mineralogist, but I recall
that aluminum is supposed to be quite stoichiometric (1:1) with Si in
kaolinite. If you were trying to determine the purity of the clay, I would
think you would look for other elements besides Al and Si rather than
trying to find the difference between Al and Si content (but you didn't say
anything about Si anyway). If I was looking at purity, I think I would use
XRF on Al and Si and the possible contaminants (Na, Mg, K, Ca, Fe, etc.). I
would probably also so some diffraction to look for other minerals. I just
can't think of a reason to do Al by AA, not that I know how to do AA anyway.

Curious to hear more,
Warren

At 02:50 PM 04/28/05, you wrote:

} This isn't microscopy, but...
}
} I need recommendation for a procedure to determine the Al content in kaolin
} clay by flame AA. Our "library" doesn't have any references and we are a
} little isolated from the local university...
}
} If anyone has a procedure they don't mind sharing or a citation to a
} reference book please contact me.
}
} Thanks!!!
}
} Frank Karl
} Degussa Corporation
} Akron Technical Center
} 3500 Embassy Parkway
} Suite 100
} Akron, Ohio 44333

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From MicroscopyL-request-at-ns.microscopy.com Fri Apr 29 09:56:41 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 29 Apr 2005 11:20:22 -0400
Subject: [Microscopy] job openings-Europe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was asked to post this position opportunity. Please respond, off
list to Professor Bein (see address below) directly.
Lee

Electron Microscopy at the University of Munich

The Department of Chemistry and Biochemistry at the University of
Munich intends to fill two positions at the Center for Electron
Microscopy. The Center supports various research groups with projects
related to the application of electron microscopy.

a) An electron microscopist to be in charge of the Center for
Electron Microscopy. The responsibilities of this position include
examinations via scanning and transmission electron microscopy,
teaching and training of users, as well as support in the future
development of the facility. Applicants should have a degree in
physics, materials science or chemistry, an excellent background and
experience in electron microscopy, and the ability to work in an
interdisciplinary team. Salary will be according to the BAT IIa scale.

b) An engineer or physicist to be in charge of the technical support
of the electron microscopes and to study materials as well as
biological samples using transmission electron microscopy and
scanning electron microscopy (including preparation).
Applicants should have a degree in engineering or a similar
qualification and gained experience in electrical engineering and
vacuum technology. It would be desirable to have a background in
electron microscopy and materials science. Salary will be according
to the BAT III scale.

We offer a wide spectrum of activities in a dynamic environment with
a modern infrastructure and look forward to your application.
Disabled persons with the same qualifications will be given
preference. Please send your applications before May 31st, 2005 to:
Professor Thomas Bein, Department of Chemistry and Biochemistry at
the Ludwig-Maximilians-University, Munich, Butenandtstr. 5-13 (E),
81377 Munich, Tel. 089-2180-77623, Fax -77622,
tbein-at-cup.uni-muenchen.de


--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 29 10:00:49 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Fri, 29 Apr 2005 11:24:42 -0400
Subject: [Microscopy] job openings-Europe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was asked to post this position opportunity. Please respond, off
list to Professor Bein (see snail-mail address below, or e-mail
above at Cc) directly.
Lee

Electron Microscopy at the University of Munich

The Department of Chemistry and Biochemistry at the University of
Munich intends to fill two positions at the Center for Electron
Microscopy. The Center supports various research groups with projects
related to the application of electron microscopy.

a) An electron microscopist to be in charge of the Center for
Electron Microscopy. The responsibilities of this position include
examinations via scanning and transmission electron microscopy,
teaching and training of users, as well as support in the future
development of the facility. Applicants should have a degree in
physics, materials science or chemistry, an excellent background and
experience in electron microscopy, and the ability to work in an
interdisciplinary team. Salary will be according to the BAT IIa scale.

b) An engineer or physicist to be in charge of the technical support
of the electron microscopes and to study materials as well as
biological samples using transmission electron microscopy and
scanning electron microscopy (including preparation).
Applicants should have a degree in engineering or a similar
qualification and gained experience in electrical engineering and
vacuum technology. It would be desirable to have a background in
electron microscopy and materials science. Salary will be according
to the BAT III scale.

We offer a wide spectrum of activities in a dynamic environment with
a modern infrastructure and look forward to your application.
Disabled persons with the same qualifications will be given
preference. Please send your applications before May 31st, 2005 to:
Professor Thomas Bein, Department of Chemistry and Biochemistry at
the Ludwig-Maximilians-University, Munich, Butenandtstr. 5-13 (E),
81377 Munich, Tel. 089-2180-77623, Fax -77622


--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Fri Apr 29 11:15:57 2005



From: pgrover :      pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 29 Apr 2005 11:40:54 -0500
Subject: [Microscopy] leaf trichomes/teliospores

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Soumitra,

I have had to collect root hairs for polysaccharide analysis of cell walls,
but I dried them first & collected them w/forceps under a dissecting scope;
major drawback was static (spend .5 hour collecting root hairs and poof,
they vanish to parts unknown. I'd think your PCR would require fresh
tissue, so why not collect cell contents with a fine needle (pulled
capillary) so you can leave the little wooden box behind? An inverted
microscope and micromanipulator would probably be best for this.

Paul Grover

-----------------------------------------------------------------------
Art is long, and critics are the insects of a day. - Randall Jarrell



Hello Everyone,

A colleague of mine is interested in removing trichomes and fungal
teliospores from grass leaf epidermis to do PCR. Is there any way she can
remove individual trichomes/teliospores while looking under a microscope
or there is some other way this can be achieved.

Any help will be highly appreciated.

Thanks,

Soumitra


*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/





From MicroscopyL-request-at-ns.microscopy.com Fri Apr 29 18:53:28 2005



From: Elaine Humphrey :      ech-at-interchange.ubc.ca
Date: Fri, 29 Apr 2005 17:18:17 -0700
Subject: [Microscopy] international cryo em course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

the fourth International Cryo EM course is June 7 - 16, 2005

at the University of British Columbia, Vancouver, Canada
hosted by the Bio-Imaging Facility

High pressure freezing, freeze substitution, cryo ultramicrotomy,
Tokuyasu method, cryo sem, cryo tem, immunolabelling and microwave
processing.

This is a hands-on course full of practical details where
participants can try their own specimens/specimen types from high
pressure freezing through to presentation of results and compare
immunolabelling with the Tokuyasu method and high pressure frozen
material.

An International Faculty includes
Kent McDonald (Berkeley)
George Postuma (Utrecht)
Helmut Gnaegi (Diatome)
Robert Apkarian (Emory)
Doug Keene (Shriners, Portland)
Randy Tindall (Missouri)
Lacey samuels (UBC)
Elaine Humphrey (UBC)
Paul de George (Leica)
Andres Kaech (Bal-Tec)

For more information e-mail Elaine Humphrey - ech-at-interchange.ubc.ca



--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 30 08:16:05 2005



From: Sharon Coe :      slc6-at-lehigh.edu (by way of MicroscopyListserver)
Date: Sat, 30 Apr 2005 08:41:09 -0500
Subject: [Microscopy] viaWWW: Lehigh Microscopy School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Only five weeks left to register for the Lehigh Microscopy School!!

June 5-17, 2005
Lehigh University
Bethlehem, PA USA

Courses include:

Introduction to SEM and EDS for the New SEM Operator (June 5)

Scanning Electron Microscopy and X-ray Microanalysis (June 6-10)

Problem Solving with Scanning Electron Microscopy (June 13-17)

Quantitative X-ray Microanalysis of Bulk Specimens and Particles (June 13-17)

Analytical Electron Microscopy (June 13-16)

Focused Ion Beam Instrumentation and Applications (June 13-16)

Particle and Fiber Characterization (June 13-16)

Scanning Probe Microscopy: from Fundamentals to Advanced Applications (June 6-9)

Telephone: (610) 758-5133
Fax: (610) 758-4244
URL: www.lehigh.edu/microscopy

For additional information please contact:


********************************
Sharon L. Coe
Events Coordinator
Lehigh Microscopy School
5 East Packer Avenue
Bethlehem, PA 18015
610-758-5133 (phone)
610-758-4244 (fax)
slc6-at-lehigh.ed
www.lehigh.edu/microscopy

********************************


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 30 08:41:42 2005



From: nair.ashwin-at-gmail.com (by way of MicroscopyListserver)
Date: Sat, 30 Apr 2005 09:06:48 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Transmission Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (nair.ashwin-at-gmail.com) from http://www.msa.microscopy.com/MLFormMail.html on Thursday, April 28, 2005 at 22:27:29
---------------------------------------------------------------------------

Email: nair.ashwin-at-gmail.com
Name: Ashwin Nair

Organization: University of Texas at Arlington

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I would like to know if there were any Transmission Electron Microscopy labs in and around Dallas-Fort Worth area. I hope you can help me as it is very crucial from my project's point of view.
Looking forward to a favorable response.


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 30 08:42:19 2005



From: cmagnus-at-virginia.edu (by way of MicroscopyListserver)
Date: Sat, 30 Apr 2005 09:07:22 -0500
Subject: [Microscopy] viaWWW: file naming and cataloging of digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cmagnus-at-virginia.edu) from http://www.msa.microscopy.com/MLFormMail.html on Friday, April 29, 2005 at 07:49:34
---------------------------------------------------------------------------

Email: cmagnus-at-virginia.edu
Name: Chris Magnus

Organization: UVa

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Are there any standardised systems for file naming and cataloging of digital images on computer which you could recommend as useful for a lab seeking to standardise its recording of microscope images?

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Sat Apr 30 10:10:54 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sat, 30 Apr 2005 10:36:18 -0500
Subject: [Microscopy] Administrivia: Nestor saw the spam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues....

Yep, I saw the spam message that go through, the rejection software has been updated.

Nestor
Your Friendly Neighborhood SysOp.


From MicroscopyL-request-at-ns.microscopy.com Sat Apr 30 10:56:56 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Sat, 30 Apr 2005 10:19:46 -0600
Subject: [Microscopy] viaWWW: file naming and cataloging of digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Chris,

Having worked in the digital imaging field for over ten years, I have not
run into a "standard naming system" for images. There are way to many
parameters that affect images and you could never encode all of them into an
image name. Just think of all the parameters one would have to use to encode
all parameters for digital images from fluorescence microscopy, SEM, TEM,
AFM, Xray, and the list goes on and on.

If you want to encode the information in the image itself, use a format like
TIF, which allows metadata to be included in the image file as "tags". The
drawback about this technique is, that again there are no public standards
for these tags, and you may have to program your own code to do this.

The right way to do this is to use a database for image storage (you can
check out our web site to see how we do that:
http://www.soft-imaging.com/rd/english/3079.htm, but a lot of other
applications have similar features). With an application like that, you set
up a database with all the parameters you want to track. You then insert the
image into the database, provide the parameters, and you're done. At a later
point, you can search the database for images that posess a certain set of
parameters (for example: All images of grains in steel taken between two
dates for customer X, or all fluorescence images taken by person Y for
Experiment Z in March).

In most cases you can store the database on a server PC and many people can
work and contribute to the database at the same time, allowing seamless
collaboration. It can also be used to distribute images from a database over
the internet to colleagues across the world
(http://www.soft-imaging.com/rd/english/420.htm).

Contact me off-line if you need more information.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: by way of MicroscopyListserver [mailto:cmagnus-at-virginia.edu]
Sent: Saturday, April 30, 2005 08:07
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cmagnus-at-virginia.edu) from
http://www.msa.microscopy.com/MLFormMail.html on Friday, April 29, 2005 at
07:49:34
---------------------------------------------------------------------------

Email: cmagnus-at-virginia.edu
Name: Chris Magnus

Organization: UVa

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Are there any standardised systems for file naming and cataloging
of digital images on computer which you could recommend as useful for a lab
seeking to standardise its recording of microscope images?

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Sat Apr 30 19:39:00 2005



From: Bill Mollon :      bmollon-at-pacbell.net
Date: Sat, 30 Apr 2005 18:03:49 -0700 (PDT)
Subject: [Microscopy] Re: file naming and cataloging of digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chris,

And while your looking, please check out
http://www.mediacy.com/iqbase/iqbase.htm. Their
digital imaging program also offers interfacing to the
database that will be keeping track of your images,
via a web browser. Very nice system.

--- Mike Bode {Mike.Bode-at-soft-imaging.net} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Hello Chris,
}
} Having worked in the digital imaging field for over
} ten years, I have not
} run into a "standard naming system" for images.
} There are way to many
} parameters that affect images and you could never
} encode all of them into an
} image name. Just think of all the parameters one
} would have to use to encode
} all parameters for digital images from fluorescence
} microscopy, SEM, TEM,
} AFM, Xray, and the list goes on and on.
}
} If you want to encode the information in the image
} itself, use a format like
} TIF, which allows metadata to be included in the
} image file as "tags". The
} drawback about this technique is, that again there
} are no public standards
} for these tags, and you may have to program your own
} code to do this.
}
} The right way to do this is to use a database for
} image storage (you can
} check out our web site to see how we do that:
} http://www.soft-imaging.com/rd/english/3079.htm, but
} a lot of other
} applications have similar features). With an
} application like that, you set
} up a database with all the parameters you want to
} track. You then insert the
} image into the database, provide the parameters, and
} you're done. At a later
} point, you can search the database for images that
} posess a certain set of
} parameters (for example: All images of grains in
} steel taken between two
} dates for customer X, or all fluorescence images
} taken by person Y for
} Experiment Z in March).
}
} In most cases you can store the database on a server
} PC and many people can
} work and contribute to the database at the same
} time, allowing seamless
} collaboration. It can also be used to distribute
} images from a database over
} the internet to colleagues across the world
} (http://www.soft-imaging.com/rd/english/420.htm).
}
} Contact me off-line if you need more information.
}
} mike
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: by way of MicroscopyListserver
} [mailto:cmagnus-at-virginia.edu]
} Sent: Saturday, April 30, 2005 08:07
} To: microscopy-at-microscopy.com
} Subject: [Microscopy] viaWWW: file naming and
} cataloging of digital
} images
}
}
}
}
}
----------------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
} ---
}
} Below is the result of your feedback form
} (NJZFM-ultra-55). It was
} submitted by (cmagnus-at-virginia.edu) from
} http://www.msa.microscopy.com/MLFormMail.html on
} Friday, April 29, 2005 at
} 07:49:34
}
---------------------------------------------------------------------------
}
} Email: cmagnus-at-virginia.edu
} Name: Chris Magnus
}
} Organization: UVa
}
} Title-Subject: [Microscopy] [Filtered]
} MListserver:
}
} Question: Are there any standardised systems for
} file naming and cataloging
} of digital images on computer which you could
} recommend as useful for a lab
} seeking to standardise its recording of microscope
} images?
}
}
---------------------------------------------------------------------------
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Sun May 1 13:04:13 2005



From: tkallison-at-comcast.net (by way of MicroscopyListserver)
Date: Sun, 1 May 2005 13:32:27 -0500
Subject: [Microscopy] viaWWW: Plasma Cleaning Speciman Chamber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tkallison-at-comcast.net) from http://www.msa.microscopy.com/MLFormMail.html on Sunday, May 1, 2005 at 12:35:13
---------------------------------------------------------------------------

Email: tkallison-at-comcast.net
Name: Ted Allison

Organization: HITACHI

Title-Subject: [Microscopy] [Filtered] Plasma Cleaning Speciman Chamber

Question: I am using the Plasma cleaner on my CDSEM. Should I be using a ultra-Pure N2 to backfill chamber while plasma cleaning?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sun May 1 13:41:33 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sun, 1 May 2005 14:10:06 -0500
Subject: [Microscopy] Administrivia: April Archives on-line & MM2005 Search Engine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

The April Microscopy Listserver Archives are now on-line at:
(http://www.microscopy.com/MicroscopyListserver)

In addition the MM2005 Program Search Engine is also operational at:
(http://mm2005.microscopy.org).


Cheers...

Nestor
Your Friendly Neighborhood SysOp


From MicroscopyL-request-at-ns.microscopy.com Sun May 1 14:36:17 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Sun, 1 May 2005 15:04:20 -0500
Subject: [Microscopy] Plasma Cleaning in SEM Specimen Chamber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ted

The nature of the gas used in plasma cleaning is related to the material your trying to remove
as well as the design of the actual plasma cleaner attached to your instrument.

For Carbon based contamination an Oxygen-based plasmas are generally better. However, for
light cleaning Argon or Argon/Oxygen mixtures also works well. I generally plasma
clean my samples in an stand alone unit before looking at it in any of my FEG TEMs or SEMs.

I have not had much success using pure Nitrogen in a plasma cleaning system (although
I have used "room air" which contains alot of O2 which does the real work).
That being said, clean low pressure Nitrogen purges were a technique developed by Ron Vane
(of XEI Scientific) many years ago also to clean SEM columns. This worked reasonably well,
however, I believe the active Oxygen base plasma systems which are now available work better.

I would suggest that you look at the WWW site of XEI Scientific http://www.evactron.com/index.html who
markets plasma cleaning units that interface to EO Columns. In addition, you might
look at the WWW sites of SouthBay Technology http://www.southbaytech.com/ , SPI http://www.2spi.com/
and Fischione Instruments http://www.fischione.com/ who also sell ancilliary units (but donot attach to columns).
They have additional information on plasma cleaning which you might find useful.

Nestor
Your Friendly Neighborhood SysOp

Disclaimer:
My employer Argonne National Laboratory, holds the original patent on Plasma Cleaning systems for
use in Electron Microscope Columns and/or as stand alone ancillary devices. The above mentioned
companies are all licensee's of that patent.



} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Sun May 1 21:40:49 2005



From: Bill Mollon :      bmollon-at-pacbell.net
Date: Sun, 1 May 2005 20:08:48 -0700 (PDT)
Subject: [Microscopy] Re: Plasma Cleaning in SEM Specimen Chamber

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Having just come from the semiconductor industry, if
your application is for the chamber of a CDSEM you're
going to need something with higher wattage. MKS has
some nice products and are widely accepted in the
CDSEM market. http://www.mksinst.com/PRG1.html.
Nitrogen is not the gas youre interested in, should be
a mixture of argon and oxygen.
--- "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Ted
}
} The nature of the gas used in plasma cleaning is
} related to the material your trying to remove
} as well as the design of the actual plasma cleaner
} attached to your instrument.
}
} For Carbon based contamination an Oxygen-based
} plasmas are generally better. However, for
} light cleaning Argon or Argon/Oxygen mixtures also
} works well. I generally plasma
} clean my samples in an stand alone unit before
} looking at it in any of my FEG TEMs or SEMs.
}
} I have not had much success using pure Nitrogen in a
} plasma cleaning system (although
} I have used "room air" which contains alot of O2
} which does the real work).
} That being said, clean low pressure Nitrogen purges
} were a technique developed by Ron Vane
} (of XEI Scientific) many years ago also to clean
} SEM columns. This worked reasonably well,
} however, I believe the active Oxygen base plasma
} systems which are now available work better.
}
} I would suggest that you look at the WWW site of
} XEI Scientific http://www.evactron.com/index.html
} who
} markets plasma cleaning units that interface to EO
} Columns. In addition, you might
} look at the WWW sites of SouthBay Technology
} http://www.southbaytech.com/ , SPI
} http://www.2spi.com/
} and Fischione Instruments http://www.fischione.com/
} who also sell ancilliary units (but donot attach to
} columns).
} They have additional information on plasma cleaning
} which you might find useful.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
} Disclaimer:
} My employer Argonne National Laboratory, holds the
} original patent on Plasma Cleaning systems for
} use in Electron Microscope Columns and/or as stand
} alone ancillary devices. The above mentioned
} companies are all licensee's of that patent.
}
}
}
}
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} -------------------------------------------------------------------------------
} }
} } Below is the result of your feedback form
} (NJZFM-ultra-55). It was submitted by
} (tkallison-at-comcast.net) from
} http://www.msa.microscopy.com/MLFormMail.html on
} Sunday, May 1, 2005 at 12:35:13
}
} ---------------------------------------------------------------------------
} }
} } Email: tkallison-at-comcast.net
} } Name: Ted Allison
} }
} } Organization: HITACHI
} }
} } Title-Subject: [Microscopy] [Filtered] Plasma
} Cleaning Speciman Chamber
} }
} } Question: I am using the Plasma cleaner on my
} CDSEM. Should I be using a ultra-Pure N2 to backfill
} chamber while plasma cleaning?
} }
}
} ---------------------------------------------------------------------------
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Mon May 2 02:16:49 2005



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Mon, 2 May 2005 09:40:58 +0200
Subject: [Microscopy] Re: Stratification Analysis via EDS by varying HT.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

Nestor et al, it is important to remind everyone that quantification of
strata by HT variation does work, but you must know some details your
sample ahead of time. Of coarse it is not an easy work and cross sections
(on SEM or TEM) give a direct look at the specimen stratification. But
cross section is destructive ! RBS or X-ray reflectometry are usefull
methodes too, but each with it's one limits.

To do that work using HT variation, one need a way to modelize theoricaly
the interactions in the stratificated sample. Since a few years, some
software are avaible which can simulate such situations, and with which
one can determine the appropriate energies to pick data and fit these
data.

I know two (and use one) of such soft : Stratageme (http://www.samx.com)
and X-film (http://www.synergie4.com)), and I heard from one less
sophisticated from Noran. They use phirozed models to simulate what
happends in a stratificated sample and are able to calculate thickness of
layers and composition of these layers. One must measure the k-ratio from
the elements which are present. The unknown parameter sometimes difficult
to evaluate is the real density of le layer, which may be quite differente
from the bulk one. One can have the same element in different layers. The
interfaces beween layers are supposed to be steep, but in case of
diffusion, or a gradiaant in composition, one can introduce one or a serie
of suplementary alloy layers. With flat sample, typically MBE or sputter
coated layers on a polished substrate, it gives the best results, but I
know people in France which have done such work with success on rough
samples made by wet chemistry. The thickness range and the Z of the
elements will determine the energie range to be used. What is easy is a
comparaison between samples in a serie, where the absolute values can be
verified by an other methode, cross section for exemple.

One limiation is that these softwares cannot until now modelise situations
with particules included in a layers, like it can be done in Monte Carlo
simulation.

The steps of such analysis are the following :

-first one discribe the sample in the software, and calculate the
k-ratio versus HV curves,which discribe the variation of X-ray emission
with the primary energie. One choose than on them the right energies to do
the acquisitions. Of coarse, better is this description of the sample,
easier will be the choice of the conditions, and more accurate the
results. Two or three energies are enough. The application ingenior of my
soft tells one energy is enough, but I prefer to use 2 or 3.


-secondly one acquire the spectra at these energies, and calculate
real k-ratio, using standard reference samples of the elements. This is
much work, because one need accurate standards, what is not always easy.
As Ritchie asked, what sample is good as standard for O, in particular
when one must work at 3 or 5 keV, where a carbon layer on an oxyde will be
well seen and will give a bad backgroud shape on the low energy side of
O-K. Using WDS will give much better results, but one must have one (!)
and it's possible to do nice works with EDS too. (By the way, I work with
EDS and cold FE-SEM, the most difficult situation !) One must only work in
the drasticals conditions, with a long "time constant" of the acquiring
chain, a clean detector, monitoring the beam current, re-polishing often
each standard witch could have an oxyde layer, counting 300, 500" or more
at low enregy to have a good signal to noise ratio, etc.

-third one put the k-ratio in the software and run the fit
calculation. It's an iterative procedure, which will stabilize more or
less fast, depending of the good "tunning" between the describtion and the
reality of the sample, and the accuracy of the measurements. But what is
interesting, is thait if one start with different "false" describtions of
the sample, good measurements will converge to the same final situation.

I've done such work for example on series of magnetic multilayers such as
Fe25Ni25Pt50 (nominal) 50 nm layers on Mgo, after annealing. Here are
results for two samples :

Fe %at Ni %at Pt %at thickness (nm)
fnp11 28 30 41.9 42.9
fnp12 25.7 32.5 41.8 42.1

The energies were 4, 8 and 12 keV.

An other case was with FePt alloys on MgO, with a Pd or Pt coverlayer and
with or without a Pt buffer between the MgO substrate and the alloy.
Thickness are 5 nm cover, 50 nm layer and 5-10 nm buffer. The results in
one case were interesting : the sample should be 5 nm Pt, 50 nm PtFe, 10
nm Pt buffer, on MgO. The results is : one 61 nm layer of Fe43-Pt56 ! The
buffer and the cover mixed during the anealing with the alloy layer. Of
coarse, a gradiant couldn't be seen. One must perform RBS for that. X-ray
reflectometry was unable to detect that. In such exemples, the density
varies much with the Pt concentration.

OK, that all is a lot of work, time consuming, and in somes cases, when
the combination line-energie/primary energie/depth doesn't match, or when
one have multiple line supperpositions (Pt-N with Pd-M and C-K !!!), it
don't work.

Last but not least, these software are expensive, and with a quite
"relative" ergonomy ! But they work, and it's what we need !

Hope it's help, and feel free to ask for more info.


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Thu, 28 Apr 2005, Nestor J. Zaluzec wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Stratification Analysis vai EDS by varying HT.
}
} Debbie
}
} IMHO, this is an inadvisable approach and will be potentially
} frought with problems and inaccuracies. I certainly would
} only try this as an absolute last resort. There are much better
} and more accurate approaches.
}
} The simpliest would be for your user to make a cross-section of the sample,
} (s)he can then image and analyze the respective strata by XEDS
} using conventional approaches, geometries and correction factors.
}
} Talk to a Materials Scientist/Metallurgist at Purdue's Materials
} Engineering / Microstructural Analysis Facility. They should
} be able to help you, as this is a routine procedure in materials
} characterization.
}
} Nestor
} Your Friendly Neighborhood SysOp.
}
}
}
}
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} } Along these lines, I was recently asked if you could get an idea about
} } stratification of different elements in a sample using EDS by adjusting the
} } kV so that the beam would penetrate to different depths and then comparing
} } the resulting spectra. The investigator expects that when a particular
} } material (primarily light elements with some Zn and Mn of interest) dries
} } down, some of the components will settle at different rates based on
} } particle size and composition. He would be content with some very general
} } data that would confirm or reject his theory.
} }
} } Is this possible or reasonable to get the desired information? Would Monte
} } Carlo simulation be able to predict this type of information and help in
} } determining the necessary sample thickness to make the results meaningful?
} }
} } Debby
} }
} } Debby Sherman, Manager Phone: 765-494-6666
} } Life Science Microscopy Facility FAX: 765-494-5896
} } Purdue University E-mail: dsherman-at-purdue.edu
} } S-052 Whistler Building
} } 170 S. University Street
} } West Lafayette, IN 47907
} } http://www3.agriculture.purdue.edu/microscopy
} }
}




From MicroscopyL-request-at-ns.microscopy.com Mon May 2 05:06:15 2005



From: sgkcck-at-aol.com
Date: Mon, 02 May 2005 06:34:03 -0400
Subject: [Microscopy]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are very glad to announce that for all of you that have been having
problems with the smoothness of the surface as well as the thickness of
the carbon tabs, 12 and 25 mm, we have a solution. After months of
research and testing we have developed and have a tab which eliminates
issues with rough surfaces, insufficient tackiness, hardness, and they
have significant lower contaminant levels under EDS. These tabs mimic
the old style tabs which became obsolete over one year ago. The part
number for the New Improved Tabs are as follows:
For the 12mm 77827-12
For the 25mm 77827-25

For more information please do not hesitate to contact us We look
forward to hearing from you

Sincerely,

Stacie Kirsch
Electron Microscopy Sciences
P.O. Box 550
1560 Industry Road
Hatfield, Pa 19440
Tel: 215-412-8400 Fax: 215-412-8450



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 05:06:35 2005



From: sgkcck-at-aol.com
Date: Mon, 02 May 2005 06:34:03 -0400
Subject: [Microscopy]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are very glad to announce that for all of you that have been having
problems with the smoothness of the surface as well as the thickness of
the carbon tabs, 12 and 25 mm, we have a solution. After months of
research and testing we have developed and have a tab which eliminates
issues with rough surfaces, insufficient tackiness, hardness, and they
have significant lower contaminant levels under EDS. These tabs mimic
the old style tabs which became obsolete over one year ago. The part
number for the New Improved Tabs are as follows:
For the 12mm 77827-12
For the 25mm 77827-25

For more information please do not hesitate to contact us We look
forward to hearing from you

Sincerely,

Stacie Kirsch
Electron Microscopy Sciences
P.O. Box 550
1560 Industry Road
Hatfield, Pa 19440
Tel: 215-412-8400 Fax: 215-412-8450



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 08:06:01 2005



From: pgrover :      pgrover-at-bilbo.bio.purdue.edu
Date: Mon, 2 May 2005 08:34:07 -0500
Subject: [Microscopy] re: file naming and cataloging of digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is just a specific example of a new problem we'll all have to face in
many aspects of our lives. With digital memory becoming exponentially
cheaper, it will soon (if not already) be more-or-less 'free' to store just
about everything - still photographs, video, audio recordings, DNA
sequences, etc. (BIG BROTHER'S COMING - AAAAAARRRRGGGGGHHHH). But, how to
find what you want (cataloging, screening, parsing ... maybe we need a new
name). And we definitely need some bright young cybergeeks to work on this
(as if they aren't already).

Paul

---------------------------------------------------------------------
Art is long, and critics are the insects of a day. - Randall Jarrell




From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:30:41 2005



From: cmagnus-at-virginia.edu (by way of MicroscopyListserver)
Date: Sat, 30 Apr 2005 09:07:22 -0500
Subject: [Microscopy] viaWWW: file naming and cataloging of digital images

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cmagnus-at-virginia.edu) from http://www.msa.microscopy.com/MLFormMail.html on Friday, April 29, 2005 at 07:49:34
---------------------------------------------------------------------------

Email: cmagnus-at-virginia.edu
Name: Chris Magnus

Organization: UVa

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Are there any standardised systems for file naming and cataloging of digital images on computer which you could recommend as useful for a lab seeking to standardise its recording of microscope images?

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:40:57 2005



From: rpowell-at-nanoprobes.com (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 17:14:50 -0500
Subject: [Microscopy] viaWWW: Job Opening Microscopy Technician, Nanoprobes Inc

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rpowell-at-nanoprobes.com) from on Wednesday, April 27, 2005 at 14:04:44
---------------------------------------------------------------------------

Email: rpowell-at-nanoprobes.com
Name: Rick Powell

Organization: Nanoprobes, Inc

Title-Subject: [Microscopy] [Filtered] Vacancy: Microscopy Technician, Nanoprobes Inc.

Question: Nanoprobes, Incorporated is a developer and manufacturer of immunogold probes and reagents located on Long Island, NY.

We are looking for a MICROSCOPY TECHNICIAN (recent Associates or BS). We need someone to help us keep our TEM up and running, then use TEM, light and fluorescence microscopy to evaluate prototypes and new products.

Job functions:

(1) Maintain and operate our TEM, schedule and coordinate repairs, maintain and manage ancillary facillities - darkroom, processing equipment and chemicals, and film.

(2) Transmission electron microscopy of samples including gold and other metal nanoparticles, autometallographically enhanced gold, and biological specimens stained or labeled with these reagents; negative staining and counterstaining as required.

(3) Help us acquire and set up lab and equipment for biological specimen processing (embedding, sectioning, etc.), then apply these methods to develop systems in which to test new staining and immunolabeling reagents.

(4) Light microscopy and fluorescent microscopy, including correlative light/electron and fluorescence/electron microscopy.

You might also work with some other biological immunostaining and detection applications (blots, gels), depending on the workload for microscopy.

We are looking for someone who can develop standard procedures for microscope operation, and for staining procedures for use as test systems to evaluate new reagents. The successful applicant will also help us implement systems for archiving and sharing microscopic data and images.

If this is you, please fax your resume to (631) 980-3608, or e-mail rpowell-at-nanoprobes.com. Unfortunately we can't offer relocation, so local candidates will be preferred.

*************************************************************
NANOPROBES, Incorporated * www.nanoprobes.com
95 Horse Block Road, Yaphank, NY 11980-9710, USA
*************************************************************

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:34:07 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 27 Apr 2005 19:11:09 -0500
Subject: [Microscopy] Re: Objectives & cover glass

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Hi, Michael

Coverslips are part of the optical train. If the system calls for "no
coverslip" and you use one, you will get spherical abberrations (lack of
focus, hazy image).

For your transmitted light work, I would recommend using a coverslip
(#1-1/2). For your reflected light work, no coverslip. The objectives you
use can also be y our guide.

Hope this is helpful.

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to
learn more about "Optimizing LIght Microscopy". Copies still available
through MME... even for class-room lots ... and we give quantity discounts.


At 05:14 AM 4/26/2005, michael shaffer wrote:


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From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:31:02 2005



From: nair.ashwin-at-gmail.com (by way of MicroscopyListserver)
Date: Sat, 30 Apr 2005 09:06:48 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Transmission Electron

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (nair.ashwin-at-gmail.com) from http://www.msa.microscopy.com/MLFormMail.html on Thursday, April 28, 2005 at 22:27:29
---------------------------------------------------------------------------

Email: nair.ashwin-at-gmail.com
Name: Ashwin Nair

Organization: University of Texas at Arlington

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I would like to know if there were any Transmission Electron Microscopy labs in and around Dallas-Fort Worth area. I hope you can help me as it is very crucial from my project's point of view.
Looking forward to a favorable response.


---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:30:14 2005



From: Sharon Coe :      slc6-at-lehigh.edu (by way of MicroscopyListserver)
Date: Sat, 30 Apr 2005 08:41:09 -0500
Subject: [Microscopy] viaWWW: Lehigh Microscopy School

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Only five weeks left to register for the Lehigh Microscopy School!!

June 5-17, 2005
Lehigh University
Bethlehem, PA USA

Courses include:

Introduction to SEM and EDS for the New SEM Operator (June 5)

Scanning Electron Microscopy and X-ray Microanalysis (June 6-10)

Problem Solving with Scanning Electron Microscopy (June 13-17)

Quantitative X-ray Microanalysis of Bulk Specimens and Particles (June 13-17)

Analytical Electron Microscopy (June 13-16)

Focused Ion Beam Instrumentation and Applications (June 13-16)

Particle and Fiber Characterization (June 13-16)

Scanning Probe Microscopy: from Fundamentals to Advanced Applications (June 6-9)

Telephone: (610) 758-5133
Fax: (610) 758-4244
URL: www.lehigh.edu/microscopy

For additional information please contact:


********************************
Sharon L. Coe
Events Coordinator
Lehigh Microscopy School
5 East Packer Avenue
Bethlehem, PA 18015
610-758-5133 (phone)
610-758-4244 (fax)
slc6-at-lehigh.ed
www.lehigh.edu/microscopy

********************************



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:33:14 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 28 Apr 2005 08:53:03 +1200
Subject: [Microscopy] Re: viaWWW: EDS Accuracy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



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Hi

I suspect that your experience illustrates more the problems with quantitative oxygen
analysis (by EDS) than with the analysis of nonconducting samples.

EDS can and does give very good quantitative analytical results, for elements from
sodium up, for all sorts of silicates, most if not all of which are nonconducting.

What oxygen standard(s) are you using?

cheers

rtch


}
} Email: pmccurdy-at-lamar.colostate.edu
} Name: Pat McCurdy
}
} Organization: Colorado State University
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: To Whom It May Concern:
}
} What accuracy should I expect from my EDS system when analyzing
} non-conducting samples? I have consistently been getting a ratio of
} 1:2.5 for Si to O when analyzing a well-grounded piece of quartz. The
} applications guy for our system told me even if I coat the outside I
} will still get charging in the bulk which will skew my results. I
} tried to take into account the charging by looking at where the
} bremsstrahlung tailed off and adjusting the accelerating voltage by an
} appropriate amount. Still the results show an oxygen-to-silicon ratio
} greater than two. Any help would be greatly appreciated.
}
} Sincerely,
}
} Pat McCurdy
} Research Scientist
} Colorado State University
}
}
} ----------------------------------------------------------------------
} -----
}

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand




From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:41:33 2005



From: monica.iliescu-at-polymtl.ca (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 17:14:18 -0500
Subject: [Microscopy] viaWWW: SEM, samples of blood clots

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (monica.iliescu-at-polymtl.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, April 27, 2005 at 07:51:45
---------------------------------------------------------------------------

Email: monica.iliescu-at-polymtl.ca
Name: Monica ILIESCU

Organization: Ecole Polytechnique

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello all:

I would like to learn if someone of you imagined, in conventional high vacuum SEM, samples of blood clots, and if yes, how proceed for the specimen preparation.

Thank you and best greetings,

Monica

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:41:15 2005



From: shaenon-at-hotmail.com (by way of MicroscopyListserver)
Date: Wed, 27 Apr 2005 17:15:18 -0500
Subject: [Microscopy] viaWWW: softening insect cuticle for histological work

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (shaenon-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, April 27, 2005 at 14:50:27
---------------------------------------------------------------------------

Email: shaenon-at-hotmail.com
Name: Shannon Mahony

Organization: Carleton University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi, I was wondering if anyone has any suggestions for softening insect cuticle for histological work. I am having much difficulty sectioning insect ears as the cuticle is so hard. Any ideas or suggestion?

Thanks in advance!

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:47:53 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 28 Apr 2005 00:10:04 -0500
Subject: [Microscopy] Stratification Analysis via EDS by varying HT.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



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Stratification Analysis vai EDS by varying HT.

Debbie

IMHO, this is an inadvisable approach and will be potentially
frought with problems and inaccuracies. I certainly would
only try this as an absolute last resort. There are much better
and more accurate approaches.

The simpliest would be for your user to make a cross-section of the sample,
(s)he can then image and analyze the respective strata by XEDS
using conventional approaches, geometries and correction factors.

Talk to a Materials Scientist/Metallurgist at Purdue's Materials
Engineering / Microstructural Analysis Facility. They should
be able to help you, as this is a routine procedure in materials
characterization.

Nestor
Your Friendly Neighborhood SysOp.




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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:53:58 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 27 Apr 2005 20:49:51 -0500
Subject: [Microscopy] Re: Re: viaWWW: EDS Accuracy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



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Along these lines, I was recently asked if you could get an idea about
stratification of different elements in a sample using EDS by adjusting the
kV so that the beam would penetrate to different depths and then comparing
the resulting spectra. The investigator expects that when a particular
material (primarily light elements with some Zn and Mn of interest) dries
down, some of the components will settle at different rates based on
particle size and composition. He would be content with some very general
data that would confirm or reject his theory.

Is this possible or reasonable to get the desired information? Would Monte
Carlo simulation be able to predict this type of information and help in
determining the necessary sample thickness to make the results meaningful?

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy

On 4/27/05 6:09 PM, "Mary Mager" {mager-at-interchange.ubc.ca} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Dear Pat,
} The quantification of the EDS results on an SEM is complicated and very
} dependant on many factors of the SEM, EDS system and sample. The
} quantification of the very light elements is further complicated by the very
} soft nature of the x-rays, which means that not all of them are detected,
} and the very large correction factors that are calculated for atomic number
} and absorption for the elements below sodium on the periodic table. If your
} sample charges, then the apparent electron beam voltage drops as the sample
} builds up a negative charge, which changes the calculation of the correction
} factors. As a final problem, if the EDS detector gets contaminated with a
} film of oil from the SEM pumping system, the softer x-rays from the lighter
} elements get preferentially absorbed. I used to go from an oxygen peak half
} the height of the silicon on my SiO2 standard, when the window was dirty, to
} an oxygen peak twice the height of the Si, after I had cleaned the window.
} Some questions: What is your EDS take-off angle? What is your EDS window
} material? Is your SiO2 sample polished flat and exactly perpendicular to the
} beam. Is it coated with a thin layer of carbon to prevent charging or are
} you using variable pressure? Is your EDS window clean or can it be cleaned?
} Sometimes it is better to use your sample as a standard in the EDS system,
} than to try to get the EDS to produce the right numbers (standardless) for
} these materials containing very light elements. In answer to your question,
} not much accuracy.
} Good luck,
} Mary Mager
} Electron Microscopist
} Department of Materials Engineering
} University of British Columbia
} 6350 Stores Road
} Vancouver, B.C. V6T 1Z4
} CANADA
} Tel: 604-822-5648
} Fax: 604-822-3619
} e-mail: mager-at-interchange.ubc.ca
} ----- Original Message -----
} } From: "by way of MicroscopyListserver" {pmccurdy-at-lamar.colostate.edu}
} To: {microscopy-at-microscopy.com}
} Sent: Wednesday, April 27, 2005 5:47 AM
} Subject: [Microscopy] viaWWW: EDS Accuracy
}
}
} }
} }
} } --------------------------------------------------------------------------
} ----
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } --------------------------------------------------------------------------
} -----
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (pmccurdy-at-lamar.colostate.edu) from
} http://www.msa.microscopy.com/MLFormMail.html on Monday, April 25, 2005 at
} 14:06:09
} } --------------------------------------------------------------------------
} -
} }
} } Email: pmccurdy-at-lamar.colostate.edu
} } Name: Pat McCurdy
} }
} } Organization: Colorado State University
} }
} } Title-Subject: [Microscopy] [Filtered] MListserver:
} }
} } Question: To Whom It May Concern:
} }
} } What accuracy should I expect from my EDS system when analyzing
} non-conducting samples? I have consistently been getting a ratio of 1:2.5
} for Si to O when analyzing a well-grounded piece of quartz. The applications
} guy for our system told me even if I coat the outside I will still get
} charging in the bulk which will skew my results. I tried to take into
} account the charging by looking at where the bremsstrahlung tailed off and
} adjusting the accelerating voltage by an appropriate amount. Still the
} results show an oxygen-to-silicon ratio greater than two. Any help would be
} greatly appreciated.
} }
} } Sincerely,
} }
} } Pat McCurdy
} } Research Scientist
} } Colorado State University
} }
} }
} } --------------------------------------------------------------------------
} -
} }
}
}





From MicroscopyL-request-at-ns.microscopy.com Mon May 2 11:46:55 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 27 Apr 2005 20:21:25 -0700
Subject: [Microscopy] Re: viaWWW: EDS Accuracy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
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Dear Pat,
The quantification of the EDS results on an SEM is complicated and very
dependant on many factors of the SEM, EDS system and sample. The
quantification of the very light elements is further complicated by the very
soft nature of the x-rays, which means that not all of them are detected,
and the very large correction factors that are calculated for atomic number
and absorption for the elements below sodium on the periodic table. If your
sample charges, then the apparent electron beam voltage drops as the sample
builds up a negative charge, which changes the calculation of the correction
factors. As a final problem, if the EDS detector gets contaminated with a
film of oil from the SEM pumping system, the softer x-rays from the lighter
elements get preferentially absorbed. I used to go from an oxygen peak half
the height of the silicon on my SiO2 standard, when the window was dirty, to
an oxygen peak twice the height of the Si, after I had cleaned the window.
Some questions: What is your EDS take-off angle? What is your EDS window
material? Is your SiO2 sample polished flat and exactly perpendicular to the
beam. Is it coated with a thin layer of carbon to prevent charging or are
you using variable pressure? Is your EDS window clean or can it be cleaned?
Sometimes it is better to use your sample as a standard in the EDS system,
than to try to get the EDS to produce the right numbers (standardless) for
these materials containing very light elements. In answer to your question,
not much accuracy.
Good luck,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "by way of MicroscopyListserver" {pmccurdy-at-lamar.colostate.edu}
To: {microscopy-at-microscopy.com}
Sent: Wednesday, April 27, 2005 5:47 AM



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I would expect to get very good results. No matter what.
Unfortunately, this does not always happen. Some times,
it is operator error...sigh.

What KV are you using? What probe size? What specimen
current?

For light elements, low KV is fine/best. For Si and O and lower
than Si, the K alpha shells are predominant. So I figure
that you only need 4-5KV. This is what I use (5KV). I coat
the specimen with around 50A of Au/Pd or Pt. No problem.

So, when done, EDAX Genesis will produce intensity error
ratios for each detected and ID element. If the ratio is higher
than about 12% or so, the quant is probably bad. Mostly this means to me
that I made an error in Z ID. If the Genesis HPD curve
says that the IDs are correct, but intensity ratios are bad,
then either I missed something really close in Z value or
there is indeed a specimen issue. Usually it is my fault.
The intensity ratios and HPD help to sort this out.

I use SiO2 for Si and O but also X-Checker Extra BN for N. So this I think
pretty much nails Si and Al. Then, I use C and N and F
to close in on the lighter elements around O. X-Checker Extra
goes down to Be. That is useful to check all elements from
Cu on down and a test for Mn (the FWHM test).

I've not had a quant problem with Genesis. Many of these
quants have been checked against other methods. I have seen no more
than about 2-3%% or so of deviation. No guess why the difference.
Perhaps it is volumetric interaction. And of course, which is
right and which is wrong?

Also, which quant method are you using for your specimen?
The default is ZAF. Fine. If you are using bulk specimens,
I think that RhoZAF is better. For best results, PhiRhoZAF
is IMO, better. But Genesis offers SEC correction factors
to tweak the ZAF values based on whatever standard you use
and admire.

Disclaimer: I use EDAX Genesis. I do not sell it, loan it
or lease it. I am just a happy user. I also do not sell
X-Checker.

gary g.


At 05:47 AM 4/27/2005, you wrote:


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From MicroscopyL-request-at-ns.microscopy.com Mon May 2 12:02:43 2005



From: :      Colin.Veitch-at-csiro.au
Date: Thu, 28 Apr 2005 16:47:38 +1000
Subject: [Microscopy] Edwards E306 coating unit

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Hi All,

I have just had to resurrect an old E306 coating unit which hasn't been
used for a while and the manuals seem to have disappeared. The valve
handle has an in and out position, with labels of, backing, roughing,
valves closed, glow discharge, fine pumping and open. Which apply to
the in and out position, and what should be the correct pumping
sequence?

Any help would greatly be appreciated.

Thank you very much.

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.





From MicroscopyL-request-at-ns.microscopy.com Mon May 2 12:15:49 2005



From: Gerd Leitinger :      gerd.leitinger-at-meduni-graz.at
Date: Thu, 28 Apr 2005 10:14:33 +0200
Subject: [Microscopy] Re: viaWWW: Lynx Automatic Tissue Processor

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Dear Alicia,

We have had a Lynx processor for a while, so I can answer some of
your questions:

1) We do not adjust the times compared to hand processing

2) We still do the osmium fixation step by hand - we were worried of
getting an osmium deposit in the machine

3) Ethanol is all right as there is a rubber cover that closes the vessels
most of the time, but propylene oxide does evaporate more quickly. I
can not tell you whether propylene oxide is all right in long protocols
since we work with acetone dehydration and do not use propylene
oxide. You could fill the vessels up and do a test run with propylene
oxide.

4) We have to clean all the vessels and the machine with acetone after
each run. We embed in TAAB resin and are able to do the first 2
changes of 100% TAAB in the machine as long as we keep the
incubation times low.

5) We do not use the Lynx for small or fragile specimens ( such as
single cells or Vibratome sections ); I found it was rougher on the tissue
than we are when we hand process it.

I hope this has helped

Gerd

Datum: Wed, 27 Apr 2005 07:49:35 -0500
An: microscopy-at-microscopy.com
Von: Alicia.Roh-at-carolinashealthcare.org (by way of
MicroscopyListserver)
Betreff: viaWWW: Lynx Automatic Tissue
Processor

}
}
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} Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Alicia.Roh-at-carolinashealthcare.org) from
http://www.msa.microscopy.com/MLFormMail.html on Tuesday, April
26, 2005 at 11:26:56
} ---------------------------------------------------------------------------
}
} Email: Alicia.Roh-at-carolinashealthcare.org
} Name: Alicia Roh
}
} Organization: Carolinas Medical Center
}
} Title-Subject: [Microscopy] [Filtered] Lynx Automatic Tissue
Processor
}
} Question: We have recently purchased a Lynx el Automatic Tissue
Processor from EMS to help
} assist with our clinical tissue processing. It appears that this machine
has
} been utilized in other laboratories for a number of years, and we
would like to
} know if anyone has any successes/challenges they would like to
share with us.
} Currently we use an 8-hour protocol for soft tissue (prior to
embedding in 100%
} resin). Our main inquires at this moment are:
}
} 1) Do any of the hand-processed protocol times need to be adjusted
for use with
} the automatic processor?
}
} 2) Is Osmium fixation recommended with the machine, or should that
be hand
} processed separately?
}
} 3) Is there excessive evaporation of 100% ethanol and/or propylene
oxide that we
} would have to account for by increasing volume?
}
} 4) Any challenges with the resin polymerizing in the vials, or
excessive
} dripping between vial rotations?
}
} 5) Does anyone use optical lens paper to 'wrap' the specimens prior
to insertion
} into the baskets. (Sometimes our samples are small enough to fall
through the
} holes).
}
} Any advice would greatly be appreciated! Thanks!
}
}
} ---------------------------------------------------------------------------
}




Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at






From MicroscopyL-request-at-ns.microscopy.com Mon May 2 12:21:20 2005



From: moss-at-relia.net
Date: Mon, 2 May 2005 11:49:12 -0600 (MDT)
Subject: [Microscopy] small SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am posting on behalf of the daVinci Academy of Sciences and Arts, a new
charter school in Ogden Utah.

My company, Mt Ogden Scientific Services (MOSS) is establishing a
mentoring program with daVinci and the school is in need of a small SEM.
If anyone has, or knows of a working SEM, that could be donated to the
school, we would be very greatful to hear from you. This would be a fully
tax deductible donation, and full acknowlegdement of this gift would be
given, including the possibility of dedicating a science room to the
donor.

Bill McManus
Mt Ogden Scientific Services
Ogden UT 84401
877/331-6677
www.mtogdensci.com


From MicroscopyL-request-at-ns.microscopy.com Mon May 2 12:27:48 2005



From: stuart McKernan :      stuartm-at-umn.edu
Date: Thu, 28 Apr 2005 04:43:17 -0500
Subject: [Microscopy] MMS Spring Symposium

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} The Minnesota Microscopy Society invites you to its Annual MMS Spring
} Symposium to be held on Friday May 6, 2005 at the Science Museum of
} Minnesota , 120 W. Kellogg Blvd., St. Paul in the Discovery Hall
} (www.sci.mus.mn.us).
}
} This year's focus is "Cutting Edge Technologies in Microscopy"
}
} Schedule
} 7:30 - 8:15 AM Registration, Continental Breakfast, and Vendor Displays
} 8:15 - 9:00 AM Tom Isabell, JEOL USA, Inc.
} Trends in Electron Microscopy, A Corrected View of the Future
} 9:00 - 9:45 AM David Larson, Imago Scientific Instruments;
} Analysis of Materials on an Atomic Scale
} 9:45 - 10:30 AM Break and Vendor Displays
} 10:30 - 11:15 PM Scott Chumbley, Iowa State University
} WebSEM: Interactive, On-Line Microscopy for Education
} 11:15 - 12:00 PM Scott Chumbley and Amy Chumbley - WebSEM Demo
} 12:00 - 1:00 PM Lunch and Vendor Displays
} 1:00 - 1:30 PM Business Meeting (Society elections, Project MICRO,
} etc.)
} 1:30 - 2:15 PM Paul Voyles, University of Wisconsin, Madison
} Imaging Single Impurity Atoms with Z-contrast STEM
} 2:15 - 3:00 PM Break and Vendor Displays
} 3:00 - 3:45 PM Duane Krueger, University of St. Thomas
} Windows into Fragile Materials: Confocal Light Microscopy and ESEM
}
} Registration
} The cost of the meeting will be $75 for MMS members and $85 for
} nonmembers. For students and K-12 teachers the registration fee is
} $35. This fee includes the meeting, buffet lunch, breakfast, coffee
} breaks, and a free pass to the Museum exhibits (a $7 value).
} Registrants can pay at the door, but reservations must be made in
} advance.
}
} The Science Museum of Minnesota always has an exciting array of
} exhibits. In addition, the Omnitheater features are "Kilimanjaro" and
} "Mars 3D". Tickets to the Omnitheater are extra.
}
} You must make your reservations by Tuesday, May 3rd, and you can do so
} by contacting Robert Lundquist (robltt-at-juno.com; 763-494-7945).
} Include your name, address, and phone number or e-mail address with
} your reservation. Due to the high cost to the Society, we will have to
} bill those who make reservations but do not show.

Prof. Stuart McKernan
IT Characterization Facility, University of Minnesota            
E-mail :  stuartm-at-umn.edu
12 Shepherd Labs,                                                    
                 Office:         (612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455                    
Lab:            (612) 626-7594








From MicroscopyL-request-at-ns.microscopy.com Mon May 2 13:00:41 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 28 Apr 2005 09:01:02 -0500
Subject: [Microscopy] viaWWW: softening insect cuticle for histological

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Believe it or not, I saw somebody successfully use Nair, the cosmetic
hair remover product, for this very purpose. As I recall it was a
project at the Southern Illinois University EM facility years ago
involving serial LM sections through the abdomens of flies
(affectionately referred to as the "bug butt" project).

For what it's worth.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu




-----Original Message-----
} From: by way of MicroscopyListserver [mailto:shaenon-at-hotmail.com]
Sent: Wednesday, April 27, 2005 5:15 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (shaenon-at-hotmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday,
April 27, 2005 at 14:50:27
------------------------------------------------------------------------
---

Email: shaenon-at-hotmail.com
Name: Shannon Mahony

Organization: Carleton University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hi, I was wondering if anyone has any suggestions for
softening insect cuticle for histological work. I am having much
difficulty sectioning insect ears as the cuticle is so hard. Any ideas
or suggestion?

Thanks in advance!

------------------------------------------------------------------------
---





From MicroscopyL-request-at-ns.microscopy.com Mon May 2 13:13:15 2005



From: David Kinast :      DKinast-at-Hitschfel.com
Date: Thu, 28 Apr 2005 13:28:17 -0500
Subject: [Microscopy] LM Job posting in St. Louis, MO

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Hitschfel Instruments, Inc, the St. Louis based Olympus Microscope dealer
is seeking applicants for a microscope sales position in the St. Louis
Metro area. If you are interested and want to learn more or submit your
resume, please visit us by pasting this link into your browser:

hitschfel.com/employment.html

Thanks to all respondents and to Nestor for providing this valuable forum.



David L. Kinast
Hitschfel Instruments, Inc.
2333 South Hanley Rd.
St. Louis, MO 63144
Phone: 800/242-3501
Phone: 314/644-6660
Fax: 314/644-5877
dkinast-at-hitschfel.com
hitschfel.com





From MicroscopyL-request-at-ns.microscopy.com Mon May 2 13:06:51 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Mon, 02 May 2005 13:34:07 -0500
Subject: [Microscopy] Re: viaWWW: SEM, samples of blood clots

Contents Retrieved from Microscopy Listserver Archives
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Monica,

I would use the normal sort of processing: fix with 1.25% (maybe a
little higher) glutaraldehyde in 0.1M PO4 buffer at pH 7.4 (if this
is a mammalian system). If your clots have lots of cells in them, add
1% tannic acid (Mallinckrodt 1764 seems to work best). 2 hr or
overnight in the refrigerator. Dehydrate through an EtOH series and
critical point dry. Osmium usually isn't needed (for TEM yes) for
things like this.
IF the clot is dry, like happens on the skin, then just mount and
coat, don't bother with anything else. A wet clot does require
processing.

Phil

} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (monica.iliescu-at-polymtl.ca) from
} http://microscopy.com/MicroscopyListserver/MLFormMail.html on
} Wednesday, April 27, 2005 at 07:51:45
} ---------------------------------------------------------------------------
}
} Email: monica.iliescu-at-polymtl.ca
} Name: Monica ILIESCU
}
} Organization: Ecole Polytechnique
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: Hello all:
}
} I would like to learn if someone of you imagined, in conventional
} high vacuum SEM, samples of blood clots, and if yes, how proceed for
} the specimen preparation.
}
} Thank you and best greetings,
}
} Monica
}
} ---------------------------------------------------------------------------

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/albrecht_web/Programs/microscopy/home.html


From MicroscopyL-request-at-ns.microscopy.com Mon May 2 13:18:26 2005



From: frank.karl-at-degussa.com
Date: Thu, 28 Apr 2005 15:50:10 -0400
Subject: [Microscopy] microscopist in search of flame AA method

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Hi Colin

referring to the arrow/pointer end of the handle (opposite the bit you hold on to):


1
IN and fully CCW, arrow pointing to "VALVES CLOSED" is the off position, in which you
can switch off the rotary pump. For startup, turn on the rotary pump, let it run a minute
or so before turning the handle:

2
90 deg CW, handle pops out, arrow points to 'BACKING" is the standby position, you
can turn on the water, push the DIFF PUMP button, give the DP 30 mins or so to warm
up, load samples and fresh carbon rod while you're waiting, Pirani should reach about
10 to the -1, then:

3
Push the handle in, turn 180 deg CCW, arrow points to "ROUGHING", rotary pump
evacuates belljar while DP gets backed by ballast chamber, so don't spend too much
time in this phase in case ballast runs out of suck, just until Pirani gets to about 2 by 10
to the -1, then:

4
Turn handle CW 180 deg back to "ROUGHING", allow it to pop out, then turn a further
180 deg CW to the "OPEN" position (same position as ROUGHING but with handle out,
not in). This is the full evacuate mode, with the DP sucking on belljar and the RP
sucking on the DP, in which you can switch on the Penning gauge, and when the
vacuum is OK, you can turn on the power to the rods and do your coating.

I don't know about the Glow discharge position.

I can fax you some manual pages if you want.

cheers

rtch






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This isn't microscopy, but...

I need recommendation for a procedure to determine the Al content in kaolin
clay by flame AA. Our "library" doesn't have any references and we are a
little isolated from the local university...

If anyone has a procedure they don't mind sharing or a citation to a
reference book please contact me.

Thanks!!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

frank.karl-at-degussa.com


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.




From MicroscopyL-request-at-ns.microscopy.com Mon May 2 13:23:28 2005



From: sghoshro-at-NMSU.Edu
Date: Thu, 28 Apr 2005 14:56:26 -0600 (MDT)
Subject: [Microscopy] leaf trichomes/teliospores

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Hello Everyone,

A colleague of mine is interested in removing trichomes and fungal
teliospores from grass leaf epidermis to do PCR. Is there any way she can
remove individual trichomes/teliospores while looking under a microscope
or there is some other way this can be achieved.

Any help will be highly appreciated.

Thanks,

Soumitra


*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 13:23:40 2005



From: Beth Bray :      bbray-at-sc.rr.com
Date: Thu, 28 Apr 2005 19:18:35 -0400
Subject: [Microscopy] microscopist in search of flame AA method

Contents Retrieved from Microscopy Listserver Archives
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Hi Frank, it has been a while since I did trace metals analysis, but these
links below are a good place to start. They are from the EPA's site for
SW-846 methods. I assume that you have all the particulars for setting up
the atomic absorption instrument. It is imperative that you suppress
aluminum's tendency to ionize by adding 1000 ppm potassium as KCl: see this
link http://www.epa.gov/epaoswer/hazwaste/test/pdfs/7000a.pdf , and read
section 3.1.4:

"Ionization interferences occur when the flame temperature is
sufficiently high to generate the removal of an electron from a
neutral atom, giving a positively charged ion. This type of interference can
generally be controlled by the addition, to both standard and sample
solutions, of a large excess (1,000 mg/L) of an easily ionized element such
as K, Na, Li or Cs."

Be sure to add the KCl to both samples and standards. You will also have to
make sure your aspiration "blank" is made up so that it has the same general
concentration of acid and KCl as your digestion blank, samples and standards
will have. A quick and dirty prep for the KCl solution is to keep adding
KCl to about 100 mL of deionized (or demineralized) water until no more will
go into solution. Then add 1 mL of it to each 100 mL of your working
standard or digested sample - add it before you make the solutions up to
final volume. Make a series of standards that will bracket the
concentration of your sample. You may have to dilute your unknown to get it
into the linear range for aluminum, and if that is the case, remember to add
another mL of your saturated KCl solution. I cannot remember the linear
range for aluminum in flame AA, but 30 mg/L sticks in my mind - though it
may be higher. At any rate, that should be in the manual that came with
your instrument.

Incidentally, you can use NaCl (table salt) instead of KCl, but you will
have to put up with the fiercely bright orange light caused by the sodium.
Not a pretty sight. Really tires the eyes!

This is a good first stop to look around:
http://www.epa.gov/epaoswer/hazwaste/test/main.htm

Go to this link and check it out for the exact method you want:
http://www.epa.gov/epaoswer/hazwaste/test/pdfs/chap3.pdf

For example, Method 3005 prepares ground water and surface water samples for
total recoverable and dissolved metal determinations by FLAA, ICP-AES, or
ICP-MS. The unfiltered or filtered sample is heated with dilute HCl and HNO
prior to metal determination.

Then there is Method 3050 which prepares waste samples for total recoverable
metals determinations by FLAA and ICP-AES, or GFAA and ICP-MS depending on
the options chosen. The samples are vigorously digested in nitric acid and
hydrogen peroxide followed by dilution with either nitric or hydrochloric
acid. The method is applicable to soils, sludges, and solid waste samples.

Then go to this link for Method 7020 for the specifics for aluminum by flame
AA: http://www.epa.gov/epaoswer/hazwaste/test/pdfs/7020.pdf

If you have any questions, please feel free to email me.

Regards,
Beth Bray
bbray-at-sc.rr.com




-----Original Message-----
} From: frank.karl-at-degussa.com [mailto:frank.karl-at-degussa.com]
Sent: Thursday, April 28, 2005 3:50 PM
To: microscopy-at-msa.microscopy.com





This isn't microscopy, but...

I need recommendation for a procedure to determine the Al content in kaolin
clay by flame AA. Our "library" doesn't have any references and we are a
little isolated from the local university...

If anyone has a procedure they don't mind sharing or a citation to a
reference book please contact me.

Thanks!!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333

frank.karl-at-degussa.com


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender by
telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.






From MicroscopyL-request-at-ns.microscopy.com Mon May 2 13:16:20 2005



From: Gazda, Jerzy :      jerzy.gazda-at-ceriumlabs.com
Date: Thu, 28 Apr 2005 14:56:18 -0500
Subject: [Microscopy] Embeding PTFE

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I have a need to embed for ultramicrotomy a non-woven PTFE fibrous pad
made out of nano-fibers. Spurrs and Epo-fix do not adhere well to the
materials and ultra-sections tend to split along epoxy-pad interface.
Any suggestions will be appreciated.

Thank you in advance.

Jerzy

******************************************************
Jerzy Gazda, Ph.D. CeriumLaboratories L.L.C.

Supervising Engineer 5204 E. Ben White
Blvd. - MS 512
Austin, TX
78741
TEL: 1-800-538-8450, Ext. 51453
jerzy.gazda-at-ceriumlabs.com
******************************************************







From MicroscopyL-request-at-ns.microscopy.com Mon May 2 14:01:54 2005



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Mon, 2 May 2005 14:29:51 -0500
Subject: [Microscopy] Administrivia: Bouncing Email

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Looks as if an Email Server from a subscriber in Botswana has gone Bonkers
and is posting old mail back to the list from a few days ago.

I'm trying to put a stop to it.

Nestor
Your Friendly Neighborhood SysOp

--


From MicroscopyL-request-at-ns.microscopy.com Mon May 2 14:25:05 2005



From: AtcSEM :      atcsem-at-sbcglobal.net
Date: Mon, 2 May 2005 14:45:31 -0400
Subject: [Microscopy] Zeiss Axioskop upgrade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

Is there any not to expensive upgrades that we could do for a Zeiss Axioskop
microscope with Kontron Electronik image analysis system with automated
stage?
Currently it runs, I hope, under Win95. Any recommendations?


Regards,
Pavel Lozovyy
ATC SEM Lab
(216)692-6637
http://www.atclabs.com/SEM.htm
 
 
 
 






From MicroscopyL-request-at-ns.microscopy.com Mon May 2 15:12:39 2005



From: Michael Boucher :      mboucher-at-tc.umn.edu
Date: Mon, 2 May 2005 15:40:19 -0500
Subject: [Microscopy] Any viable market for a Jeol 100CX and Hitachi S450

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I need to dispose of these scopes. Does anyone know of a market for the
Hitachi S450 SEM (circa 1978)or Jeol 100CX TEM (circa 1980)?
I need to establish values, if any, for them.
If they have no value I can donate them or give them away; so any takers?
Both need ? minor repair I am told. The S450 has a second parts scope that
goes with it.
Thanks


Mike
******************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 18 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
MiNTeC node of the National Nanotechnology Infrastructure Network

University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://www.charfac.umn.edu
********************************************************************



From MicroscopyL-request-at-ns.microscopy.com Mon May 2 18:25:01 2005



From: moss-at-relia.net
Date: Mon, 2 May 2005 17:52:17 -0600 (MDT)
Subject: [Microscopy] Re: Any viable market for a Jeol 100CX and Hitachi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike:

I just posted today looking for a scope ( SEM) that could be donated to a
new charter school here in Ogden UT. If the Hitachi has manuals and extra
parts we can repair it. As the school is a public school this would be a
fully tax deductible contribution.

Bill McManus
877/311-6677
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} I need to dispose of these scopes. Does anyone know of a market for the
} Hitachi S450 SEM (circa 1978)or Jeol 100CX TEM (circa 1980)?
} I need to establish values, if any, for them.
} If they have no value I can donate them or give them away; so any takers?
} Both need ? minor repair I am told. The S450 has a second parts scope that
} goes with it.
} Thanks
}
}
} Mike
} ******************************************************************
} Michael L. Boucher Sr. mboucher-at-tc.umn.edu
} Lab Manager Rm 18 Office Ph 612-624-6590
} I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
} MiNTeC node of the National Nanotechnology Infrastructure Network
}
} University of MN Fax 612-625-5368
} 12 Shepherd Labs
} 100 Union Street S.E.
} Minneapolis, MN 55455 http://www.charfac.umn.edu
} ********************************************************************
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Tue May 3 06:13:35 2005



From: EMS :      sampleprep-at-earthlink.net
Date: Tue, 3 May 2005 07:41:01 -0400
Subject: [Microscopy] Re: Embeding PTFE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jerzy:

I think you have two options: Find a Silane Coupling agent that with work
with PTFE and your embedding resin of choice or CryoUltramicrotomy. Gelest
Inc.( www.gelest.com ) has a nice booklet on the properties of the ones they
offer but since I'm not a polymer chemist I'm not sure which one might work
if any. I did not see one specifically for PTFE but they can tell you if
this is a viable option or not. We use them in our Materials Microtomy
course for just such an application with fantastic results with other
materials. As for Cryoultramicrotomy; I know from personal experience that
this works quite well but requires additional hardware and a different skill
set. That's all I can think of regarding this slippery subject.


Cheers!

Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com

} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} I have a need to embed for ultramicrotomy a non-woven PTFE fibrous pad
} made out of nano-fibers. Spurrs and Epo-fix do not adhere well to the
} materials and ultra-sections tend to split along epoxy-pad interface.
} Any suggestions will be appreciated.
}
} Thank you in advance.
}
} Jerzy
}
} ******************************************************
} Jerzy Gazda, Ph.D. CeriumLaboratories L.L.C.
}
} Supervising Engineer 5204 E. Ben White
} Blvd. - MS 512
} Austin, TX
} 78741
} TEL: 1-800-538-8450, Ext. 51453
} jerzy.gazda-at-ceriumlabs.com
} ******************************************************
}
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Tue May 3 07:55:40 2005



From: Gareth Morgan :      Gareth.Morgan-at-labmed.ki.se
Date: Tue, 03 May 2005 15:24:43 +0200
Subject: [Microscopy] Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

We are using a 'home grown' software package for image analysis of digital
photomicrographs. The author works here but might move soon which makes me
think about the future.

Can anyone give me tips about good all-round image analysis software with
standard forms of analysis - feature counts, areas etc. Preferably that
doesn't cost an arm and a leg. We are using PCs here.

Thanks

Gareth

With best wishes,

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Histo/cytopathology, Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.




From MicroscopyL-request-at-ns.microscopy.com Tue May 3 08:35:49 2005



From: brian :      mcintyre-at-optics.rochester.edu
Date: Tue, 03 May 2005 10:03:29 -0400
Subject: [Microscopy] Please review student work in EM practicum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers-

In the past this forum has reviewed the work of students in the EM
Practicum class at the Univ of Rochester. We have found this helpful in a
variety of ways.

This year's class had some interesting self-proposed projects. The web
version of these projects has been posted for your review and enjoyment (as
well as comments). Please navigate
to: http://xray.optics.rochester.edu/workgroups/cml/opt307/spr05/index.html
to see them.

Thanks!
Brian McIntyre
____________________________________________________
Brian McIntyre
University of Rochester
Institute of Optics
RCEMLab
585-275-3058
585-244-4936 fax

"Be well, do good work, and keep in touch"



From MicroscopyL-request-at-ns.microscopy.com Tue May 3 08:43:50 2005



From: Beavers, Roy :      rbeavers-at-mail.smu.edu
Date: Tue, 3 May 2005 09:11:45 -0500
Subject: [Microscopy] SEM RF Interference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Group,
}
} I would like to have some input from anyone who has experienced any negative effects on SEM operation as a result of wireless systems being installed near their instruments. We have two possible systems being considered for our building. One is a wireless Internet system and the other is a GPS based wireless clock system. Some specs on the wireless clock are listed below:
}
} Frequency Range: 72.100 to 72.400 MHz.
}
} Transmission Power: 1 watt (30dBm) maximum
}
} Radio technology: narrowband FM
}
} Transmitter output power: +26 to +30 dBm
}
} Thanks
}
} Roy Beavers
}
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, TX 75275
} Voice: 214-768-2756
} Fax: 214-768-2701



From MicroscopyL-request-at-ns.microscopy.com Tue May 3 09:22:41 2005



From: Miller, Shea :      MILLERS-at-agr.gc.ca
Date: Tue, 3 May 2005 10:50:18 -0400
Subject: [Microscopy] Fluorescence: soluble phenolics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone;
I have a user who would like to look at the soluble phenolics in her tissues. We are planning on using cryosections of fresh tissue. I have some experience with using fluorescence to look at wall-bound phenolics, but I am wondering if anyone has any insights about keeping soluble phenolics in place for imaging?

thanks in advance
shea

Dr. S. Shea Miller
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1760
Facsimile/Télécopieur: 613-759-1701
Rm 2068 K.W. Neatby Bldg
960 Carling Ave.
Central Experimental Farm
Ottawa, ON
K1A 0C6
millers-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada




From MicroscopyL-request-at-ns.microscopy.com Tue May 3 09:51:57 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 03 May 2005 08:19:44 -0700
Subject: [Microscopy] User feedback on Denton Desk IV TSC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers:

I'm seeking user feedback on the turbo model of
the Denton Desk IV with or w/o Carbon yarn option.

I've been using the Denton Desk II with excellent
results. However, with a higher resolution FESEM,
I'm seeing effects of backstreaming from the mech
pump even at just 5KV. Additionally, I am seeing
the metal grains.

Any comments? Off-list is best. I'm trying to sort
out equipment options and budgets based on what is
out there.

tnx,
gary g.



From MicroscopyL-request-at-ns.microscopy.com Tue May 3 10:05:23 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 03 May 2005 08:32:51 -0700
Subject: [Microscopy] Re: SEM RF Interference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I run a wireless LAN bridge at 5GHz with no problems. It an
Airaya system. The only problem with it is aligning
the antennas. The antennas are directional. Thus, if
not pointed to the SEM, no big deal.

I can't see why you would have a problem at 72MHz.
If an antenna was pointed directly at the SEM, you might.
However, the frequency is so high the energy ought to
be sucked to ground. If this system uses omnidirectional
antennas (probably one at least at the TX side) then
distance from the TX to the SEM might be an issue.

gary g.
N6OIJ

At 07:11 AM 5/3/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Tue May 3 10:07:05 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Tue, 3 May 2005 09:33:33 -0600
Subject: [Microscopy] Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Gareth,

I suppose you will get a lot of answers now: Buy this package or buy that
package. I could do the same for our software, but rather I would like to
give you a few general pointers:

1) Make sure you know what you are looking for. Take a good look at your
workflows and what the important issues are. It would not be good to buy a
package with all the bells and whistles just to find out that it cannot do a
critical element of your workflow.

2) You may want to spend a little time with a demo version of the software
you are contemplating to see if it fits your working style. A demo is good,
but try to use the software yourself.

3) make sure that you can get support easily. If you cannot get support, you
might be in a tight spot later.

4) Make sure to know what kind of licensing and how many licenses you want.
network licensing can drastically reduce the cost.

5) Make sure the software that you purchase has programming capabilities so
you can transfer some of your home-brewed applications.

6) By the same token, make sure that you have some overlap between
installing the new software and your programmer leaving. My guess is that it
will be very hard to transfer any application from a home-made system to a
commercial system without the original programmer.

7) Make sure that the new software interfaces with your instruments and
cameras (if applicable). Put together a list and send it to the potential
vendors.

8) Check for workgroup requirements and capabilities. Do you need many
people working on the same projects? Central storage of images? Reports?

These are just my 2 cents worth of advice. Contact me off-line if you want
to discuss this further or if you want to get information about our
products.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Gareth Morgan [mailto:Gareth.Morgan-at-labmed.ki.se]
Sent: Tuesday, May 03, 2005 07:25
To: Microscopy-at-microscopy.com

Hi

We are using a 'home grown' software package for image analysis of digital
photomicrographs. The author works here but might move soon which makes me
think about the future.

Can anyone give me tips about good all-round image analysis software with
standard forms of analysis - feature counts, areas etc. Preferably that
doesn't cost an arm and a leg. We are using PCs here.

Thanks

Gareth

With best wishes,

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Histo/cytopathology, Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.






From MicroscopyL-request-at-ns.microscopy.com Tue May 3 10:44:06 2005



From: Moninger, Thomas :      thomas-moninger-at-uiowa.edu
Date: Tue, 3 May 2005 11:12:06 -0500
Subject: [Microscopy] Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gareth,

I will probably be one of many to recommend ImageJ. (http://rsb.info.nih.gov/ij/) It is essentially a Java version of the old NIH Image software and thus is cross platform-- Windows, Mac and Linux. It is quite powerful and flexible. One of the great aspects of ImageJ is it's Java platform--a lot of folks can program Java and are always updating the site with new and exciting plugins. Also, the price it right, free!! Hope this works for you.

Sköl!

Tom Moninger

-----Original Message-----
} From: Gareth Morgan [mailto:Gareth.Morgan-at-labmed.ki.se]
Sent: Tuesday, May 03, 2005 8:25 AM
To: Microscopy-at-microscopy.com

Hi

We are using a 'home grown' software package for image analysis of digital
photomicrographs. The author works here but might move soon which makes me
think about the future.

Can anyone give me tips about good all-round image analysis software with
standard forms of analysis - feature counts, areas etc. Preferably that
doesn't cost an arm and a leg. We are using PCs here.

Thanks

Gareth

With best wishes,

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Histo/cytopathology, Laboratory Medicine (Labmed),
Karolinska Institute,
Karolinska University Hospital at Huddinge, F46
SE 141 86 Stockholm
Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.






From MicroscopyL-request-at-ns.microscopy.com Tue May 3 11:16:31 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Tue, 03 May 2005 09:44:18 -0700
Subject: [Microscopy] Print Window for Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
A while back John Bozzola asked how to print out the files on a CD or
any folder for that matter since OSX doesn't do that. In the recent
MacWorld found a piece of software that does just that. There is a free
version and an advanced version.
See below for more info and URL. Have been using the free version which
works just fine. I have no financial interest in this product, but
definitely a happy user.

Judy

Judy Murphy, PhD
Microscopy Training, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net






Print Window at
http://swssoftware.com/products/printwindow/

We've brought back what Apple forgot.

Ever since Apple introduced Mac OS X, users have been complaining about
the inability to easily print a file listing from directly within the
Finder.

Enter Print Window.

Print Window offers the ability to print a file listing from directly
within the Mac OS X Finder. No more taking screenshots of window or
setting for text-only printouts of filenames only. Print Window provides
the works: icons, file information, sorting and more!



Print Window Standard Features

Print Window provides you with the ability to fully control what your
printed listings look like. You can decide whether or not to include
icons, file information and page headers. You can pre-sort file listings
by a variety of criteria. You can even print your file listings in
multiple columns on the same page!

* Icons - If you want your file listings to include icons, you can.
If you don't want icons, don't worry, they're not required. If you
do print icons, you can also decide how large you want them to be
(16x16 pixels to 128x128 pixels).
* File Information - With Print Window, you can decide if you want
your file listing to include just the file names or if you want to
print a wide variety of information about each file.
* Look Deeper - Have a folder that contains multiple sub-folders
that also have files you want to include in your file listing? No
problem! Print Window will allow you to automatically expand
subfolders so that your file listing will include those files as well.



Print Window Advanced

Print Window Advanced is new for Version 3! Along with all the other
great features in Print Window Standard, Print Window Advanced provides
even more great features to provide even greater control of the final
look of your file listings.

* CD and DVD Covers - If you've just burned a CD or DVD and want to
include a list of files found on the disk, now you can! Print
Window Advanced will print your file listing pre-formatted to fit
in a standard CD or DVD case. CD Covers will even print as a
booklet, if your listing takes up more than one finished page!
* Manual Expansion - Print Window Standard provides the ability to
expand subfolders. Print Window Advanced also allows you to
manually select what folders to expand. So, if you want to expand
some and not others, that's fine. It's your choice.
* File Information - Print Window Standard allows you to print
information for each file included in your file listing. However,
it doesn't allow you to decide what information to include. Print
Window Advanced allows you to pick and choose what information to
include about each file.


}
}
}


From MicroscopyL-request-at-ns.microscopy.com Tue May 3 15:04:54 2005



From: Richard Harris :      rjharris-at-uwo.ca
Date: Tue, 03 May 2005 16:30:31 -0400
Subject: [Microscopy] Re: Print Window for Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers
while I'm not a Power user of Macs, my colleague is and he uses a keyboard
short cut for a screen capture which can then be dumped directly to the
printer or a software package(Word, Illustrator, etc) for resizing and
printing. He uses this function all the time to print Data CD and DVD covers
and inserts.

This command works in OS 9 and OS X:
"Command key + Shift key + 4 key + spacebar" will display a camera symbol
and capture the currently active window to the clipboard and display an icon
below your drive Icon on the right hand edge of the screen. It's the
equivalent of the Windows keyboard command "Alt key + PrtScn key" to capture
the currently active window to the clipboard.
"Command key + Shift key + 4 key" will display a '+' symbol and allow you to
drag an marquee around whatever in the window you'd like to capture.
"Command key + Shift key + 3 key" will capture the whole desktop

Drag and drop the icon onto your printer or in the software package of your
choice for printing.

No extra programs needed!

Richard Harris
Laboratory Supervisor,
Imaging and Analysis
Department of Biology,
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph. 519-661-2111 ext. 86780
Fax 519-661-3935



From MicroscopyL-request-at-ns.microscopy.com Tue May 3 16:57:08 2005



From: Judy Murphy :      murphyjudy-at-comcast.net
Date: Tue, 03 May 2005 15:24:22 -0700
Subject: [Microscopy] Re: Re: Print Window for Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As Richard mentioned, for screen shots I also use image capture command
all the time, but most of my hard drives are much longer than a screen's
length and taking several screen shots is a pain, thus Print Window is a
godsend for old Mac users that are used to that function. Text Wrangler
works well (as mentioned previously to John Bozzola's request), but it
opens all the folders and doesn't print the size and kind of file out.
Different strokes for different folks.

Judy


Judy Murphy, PhD
Microscopy Training, Imaging & Lab Design Consultant
Stockton, CA 95219
murphyjudy-at-comcast.net

Richard Harris wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


From MicroscopyL-request-at-ns.microscopy.com Tue May 3 18:44:05 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Tue, 03 May 2005 20:11:56 -0400
Subject: [Microscopy] Lung EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have to embed some lung tissue into plastic and
also prepare some for cryo-ultramicrotomy and
immuno-gold labeling. I was wondering if I need to
take some precautions to prevent collapse of the
tissue during embedding and sectioning (especially
cryosectioning)?

Any advise and/or protocol would be most welcome!

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Tue May 3 20:33:28 2005



From: tiaross-at-comcast.net (by way of Ask-A-Microscopist)
Date: Tue, 3 May 2005 21:01:38 -0500
Subject: [Microscopy] AskAMicroscopist: test on architectural paint samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tiaross-at-comcast.net) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, May 3, 2005 at 09:38:46
---------------------------------------------------------------------------

Email: tiaross-at-comcast.net
Name: Tia Ross

Organization: Savannah College of Art & Design

Education: Graduate College

Location: Savannah, GA, USA

Question: I'm working on my MFA thesis in Historic Preservation and attempting to conduct the following test on architectural paint samples, looking for the presence of zinc white [the test is from an artilce written by Casas & Llopis in "Studies in Conservation" 47 (2002)]. I don't think I'll have trouble interpreting the results, but I'm unsure of how to actually measure the chemicals.

Here are the preparation instructions: "dilute sulfuric acid added to solution of potassium dichromate (1% by weight, 50ml) until the pH reaches 1.6; silver nitrate solution (0.125M, 10ml) is added slowly with vigorous stirring."

Does that mean that I add 1% by weight of sulfuric acid to 50ml of potassium dichromate? How do I measure 1% by weight? Then I add 10ml of silver nitrate? If someone could give me the 'dummies' version of how much of each chemical I need to add I'd be most grateful!


Thank you for your help,
Tia Ross


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue May 3 22:42:49 2005



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 04 May 2005 00:46:27 -0400
Subject: [Microscopy] Re: AskAMicroscopist: test on architectural paint samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tia,

Since you are not familiar with handling chemicals you should try to find a faculty member at your school to supervise this the first time that you try it. If there isn't a chemist around, put in a call to the local high school science teacher and get some tips from him or her. They can explain what a .125M (.125 Molar) solution is and how to calculate the weight of silver nitrate to make
it. More importantly, they can give you the necessary precautions in handling these things.
Each of these three chemicals is capable of doing serious damage to your eyes so wear goggles. If you have to make your own dilute sulfuric acid from the concentrated variety NEVER add water to the acid or position the bottle so that water can splash into it, as it may boil out on you.

The procedure should be understood to mean that the amount of dilute sulfuric acid is not critical, you are only using it to adjust the pH. The weights pertain to the amount of potassium dichromate in water. Since the density of water is 1.0, 50ml of water weighs 50 grams. In order to make that 50 ml have 1% by weight of dichromate in it, you need to add .01 x 50 gm = 0.5g of potassium
dichromate. Once you have that dissolved then you will add dilute sulfuric acid a drop at a time until the pH goes down to 1.6. For that you have to have some means of measuring the pH, normally a pH meter. (The dichromate is highly colored and you also can't rely on pH test paper due to the oxidizing ability of this substance.)
Next goes in 10 ml of the .125 M silver nitrate, but you have to make that up first.

John Twilley


by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------------
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} ---------------------------------------------------------------------------
}
} Email: tiaross-at-comcast.net
} Name: Tia Ross
}
} Organization: Savannah College of Art & Design
}
} Education: Graduate College
}
} Location: Savannah, GA, USA
}
} Question: I'm working on my MFA thesis in Historic Preservation and attempting to conduct the following test on architectural paint samples, looking for the presence of zinc white [the test is from an artilce written by Casas & Llopis in "Studies in Conservation" 47 (2002)]. I don't think I'll have trouble interpreting the results, but I'm unsure of how to actually measure the chemicals.
}
} Here are the preparation instructions: "dilute sulfuric acid added to solution of potassium dichromate (1% by weight, 50ml) until the pH reaches 1.6; silver nitrate solution (0.125M, 10ml) is added slowly with vigorous stirring."
}
} Does that mean that I add 1% by weight of sulfuric acid to 50ml of potassium dichromate? How do I measure 1% by weight? Then I add 10ml of silver nitrate? If someone could give me the 'dummies' version of how much of each chemical I need to add I'd be most grateful!
}
} Thank you for your help,
} Tia Ross
}
} ---------------------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Tue May 3 23:01:51 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 03 May 2005 21:29:19 -0700
Subject: [Microscopy] Re: AskAMicroscopist: test on architectural paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've looked at paint samples before and find that
they are not easy. Not all specimens are easy!!

A nice way to analyze them is an EDS line scan across the
section accompanied by a map of the section. Try this
and see what you get. BSE also helps. But if you want
elemental analysis, you will need EDS.

Initially, don't do anything to the specimen. Do
EDS/maps and see what you get. Otherwise, your specimen prep
may alter the actual specimen--Heisenberg uncertainty
principle in action.

Just a thought...

gary g.


At 07:01 PM 5/3/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Tue May 3 23:55:48 2005



From: Connie McManus :      conniemoss-at-relia.net
Date: Tue, 3 May 2005 23:23:37 -0600 (MDT)
Subject: [Microscopy] Re Test on archetectural paint samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tia,
I read your post on Microscopy.
It sounds to me like they are asking you to acidify 50 mL of a 1%
potassium dichromate solution, (prepared as a weight to volume (W/V)
solution). It's the dichromate that is weighed. The H2SO4 is added (they
use "dilute") to bring the pH down to 1.6. Then, after the pH has been
reached, add the silver nitrate solution drop by drop. Be very careful
when you do this becayse the dichromate/sulfuric acid solution can be
volatile and I'm guessing the silver will probably precipitate if it is
added too quickly.

Before you do this, be certain to wear safety goggles, nitrile gloves, a
barrier lab coat and work in a fume hood with this stuff. It's all very
nasty to breathe or get on your skin.

I hope this has been helpful to you. IMO, the wording you have given us
seems antiquated and ambiguous. I don't blame you for scratching your
head on this one! *g*

Have fun and good luck!

--
Connie McManus
Mt Ogden Scientific Services
950 W Kershaw, Suite E
Ogden UT 84401
tel: 801/334-6677
direct: 801/745-2583
cell: 435/757/2975
fax: 435/514-1781
conniemoss-at-relia.net
www.mtogdensci.com

======
--------------------------------------------------------------------------

Email: tiaross-at-comcast.net
Name: Tia Ross

Organization: Savannah College of Art & Design

Education: Graduate College

Location: Savannah, GA, USA

Question: I'm working on my MFA thesis in Historic Preservation and
attempting to
conduct the following test on architectural paint samples, looking for the
presence
of zinc white [the test is from an artilce written by Casas & Llopis in
"Studies in
Conservation" 47 (2002)]. I don't think I'll have trouble interpreting the
results,
but I'm unsure of how to actually measure the chemicals.

Here are the preparation instructions: "dilute sulfuric acid added to
solution of
potassium dichromate (1% by weight, 50ml) until the pH reaches 1.6; silver
nitrate
solution (0.125M, 10ml) is added slowly with vigorous stirring."

Does that mean that I add 1% by weight of sulfuric acid to 50ml of potassium
dichromate? How do I measure 1% by weight? Then I add 10ml of silver
nitrate? If
someone could give me the 'dummies' version of how much of each chemical I
need to
add I'd be most grateful!


Thank you for your help,
Tia Ross





From MicroscopyL-request-at-ns.microscopy.com Wed May 4 06:24:14 2005



From: frank.karl-at-degussa.com
Date: Wed, 4 May 2005 07:51:10 -0400
Subject: [Microscopy] Re: AskAMicroscopist: test on architectural paint samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tia,
I'm a little confused by your test. I checked Feigl (Inorganic Spot Test)
and was unable to find a test for zinc using these reagents. While Feigl
isn't the last word, he's a good starting place. More to the point, I
would expect your reagents to form silver chromate and silver sulfate. The
CRC handbook indicated silver sulfate has low solubility and I would expect
it to precipitate out. The formation of silver chromate is a test result
for microchemical testing for chromate. Make sure you try this test on
paint that doesn't have zinc in it and a sample you know has zinc in it.
Good luck!!

I'll have to get a copy of the article, this test has me interested.



Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
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tiaross-at-comcast.n
et (by way of To: microscopy-at-microscopy.com
Ask-A-Microscopis cc:
t) Subject: [Microscopy] AskAMicroscopist: test on architectural paint samples

05/03/2005 10:01
PM







------------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tiaross-at-comcast.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on
Tuesday, May 3, 2005 at 09:38:46
---------------------------------------------------------------------------

Email: tiaross-at-comcast.net
Name: Tia Ross

Organization: Savannah College of Art & Design

Education: Graduate College

Location: Savannah, GA, USA

Question: I'm working on my MFA thesis in Historic Preservation and
attempting to conduct the following test on architectural paint samples,
looking for the presence of zinc white [the test is from an artilce written
by Casas & Llopis in "Studies in Conservation" 47 (2002)]. I don't think
I'll have trouble interpreting the results, but I'm unsure of how to
actually measure the chemicals.

Here are the preparation instructions: "dilute sulfuric acid added to
solution of potassium dichromate (1% by weight, 50ml) until the pH reaches
1.6; silver nitrate solution (0.125M, 10ml) is added slowly with vigorous
stirring."

Does that mean that I add 1% by weight of sulfuric acid to 50ml of
potassium dichromate? How do I measure 1% by weight? Then I add 10ml of
silver nitrate? If someone could give me the 'dummies' version of how much
of each chemical I need to add I'd be most grateful!


Thank you for your help,
Tia Ross


---------------------------------------------------------------------------






From MicroscopyL-request-at-ns.microscopy.com Wed May 4 09:05:39 2005



From: Ron L'Herault :      lherault-at-bu.edu
Date: Wed, 4 May 2005 10:34:23 -0400
Subject: [Microscopy] Re: AskAMicroscopist: test on architectural paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tia,

A book that you may find very helpful is "Laboratory Mathematics", subtitled
Medical and Biological Applications, by June and Joe Campbell. It
addresses concentration and solutions among other things.

Ron L

-----Original Message-----
} From: John Twilley [mailto:jtwilley-at-sprynet.com]
Sent: Wednesday, May 04, 2005 12:46 AM
To: tiaross-at-comcast.net
Cc: microscopy-at-microscopy.com

Tia,

Since you are not familiar with handling chemicals you should try to find a
faculty member at your school to supervise this the first time that you try
it. If there isn't a chemist around, put in a call to the local high school
science teacher and get some tips from him or her. They can explain what a
125M (.125 Molar) solution is and how to calculate the weight of silver
nitrate to make
it. More importantly, they can give you the necessary precautions in
handling these things.
Each of these three chemicals is capable of doing serious damage to your
eyes so wear goggles. If you have to make your own dilute sulfuric acid
from the concentrated variety NEVER add water to the acid or position the
bottle so that water can splash into it, as it may boil out on you.

The procedure should be understood to mean that the amount of dilute
sulfuric acid is not critical, you are only using it to adjust the pH. The
weights pertain to the amount of potassium dichromate in water. Since the
density of water is 1.0, 50ml of water weighs 50 grams. In order to make
that 50 ml have 1% by weight of dichromate in it, you need to add .01 x 50
gm = 0.5g of potassium
dichromate. Once you have that dissolved then you will add dilute sulfuric
acid a drop at a time until the pH goes down to 1.6. For that you have to
have some means of measuring the pH, normally a pH meter. (The dichromate
is highly colored and you also can't rely on pH test paper due to the
oxidizing ability of this substance.)
Next goes in 10 ml of the .125 M silver nitrate, but you have to make that
up first.

John Twilley


by way of Ask-A-Microscopist wrote:

} --------------------------------------------------------------------------
----
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
-----
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tiaross-at-comcast.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on
Tuesday, May 3, 2005 at 09:38:46
} --------------------------------------------------------------------------
-
}
} Email: tiaross-at-comcast.net
} Name: Tia Ross
}
} Organization: Savannah College of Art & Design
}
} Education: Graduate College
}
} Location: Savannah, GA, USA
}
} Question: I'm working on my MFA thesis in Historic Preservation and
attempting to conduct the following test on architectural paint samples,
looking for the presence of zinc white [the test is from an artilce written
by Casas & Llopis in "Studies in Conservation" 47 (2002)]. I don't think
I'll have trouble interpreting the results, but I'm unsure of how to
actually measure the chemicals.
}
} Here are the preparation instructions: "dilute sulfuric acid added to
solution of potassium dichromate (1% by weight, 50ml) until the pH reaches
1.6; silver nitrate solution (0.125M, 10ml) is added slowly with vigorous
stirring."
}
} Does that mean that I add 1% by weight of sulfuric acid to 50ml of
potassium dichromate? How do I measure 1% by weight? Then I add 10ml of
silver nitrate? If someone could give me the 'dummies' version of how much
of each chemical I need to add I'd be most grateful!
}
} Thank you for your help,
} Tia Ross
}
} --------------------------------------------------------------------------
-







From MicroscopyL-request-at-ns.microscopy.com Wed May 4 12:51:58 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 04 May 2005 13:18:58 -0500
Subject: [Microscopy] Re: Lung EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Marc

You might investigate the vacuum mounting system from Buehler. It is great for infiltration of delicate tissue like this.

Caveat: MME has no vested interest in this product.

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.


At 07:11 PM 5/3/2005, Marc Pypaert wrote:


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From MicroscopyL-request-at-ns.microscopy.com Wed May 4 12:53:18 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 04 May 2005 13:20:16 -0500
Subject: [Microscopy] Re: AskAMicroscopist: test on architectural paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tia,

Just a reminder that if you are going to do any optical microscopy on cross-sections, darkfield is the way to go.

Thanks,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.



At 09:01 PM 5/3/2005, tiaross-at-comcast.net wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Wed May 4 13:36:17 2005



From: AtcSEM :      atcsem-at-sbcglobal.net
Date: Wed, 4 May 2005 14:30:13 -0400
Subject: [Microscopy] Zeiss microscope Marzhauser Stage controller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Has anyone upgraded Marzhauser motorized stage to WinXp from Win95. I’m
trying to find inexpensive solution for the scope.
 
 
Thanks,
Pavel Lozovyy
ATC SEM Lab
(216)692-6637
http://www.atclabs.com/SEM.htm
 




From MicroscopyL-request-at-ns.microscopy.com Wed May 4 23:15:39 2005



From: Jacob Bastacky :      jbastacky-at-chori.org
Date: Wed, 4 May 2005 21:43:27 -0700
Subject: [Microscopy] Re: Lung EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marc,

What you need to do depends on what your concerns with collapse are.
If you want to maintain the more than 80% of the lung that is air as
empty space, you can freeze the lung while it is inflated. Freezing
will be relatively slow, however. If you want to examine the structure
of the cells and tissue components, you will get smaller ice voids if
you allow the lung to collapse (become nearly airless) and freeze then.
For plastic embedding you can inflate the lung with liquid fixative (
we use 2.3% glutaraldehyde in sodium cacodylate paying attention to the
osmolarity, pH, and inflation pressure. Details if you wish.

Jacob

} } ----------------------------------------------------------------------
} } ---------
} }
} } I have to embed some lung tissue into plastic and
} } also prepare some for cryo-ultramicrotomy and
} } immuno-gold labeling. I was wondering if I need to
} } take some precautions to prevent collapse of the
} } tissue during embedding and sectioning (especially
} } cryosectioning)?
} }
} } Any advise and/or protocol would be most welcome!
} }
} } Marc
} }
} } --
} } Marc Pypaert
} } Department of Cell Biology
} } Center for Cell and Molecular Imaging
} } Ludwig Institute for Cancer Research
} } Yale University School of Medicine
} } 333 Cedar Street, PO Box 208002
} } New Haven, CT 06520-8002
} } TEL 203-785 3681
} } FAX 203-785 7446
} }
}
}
}
}
Jacob Bastacky, M.D.
Associate Scientist
Children's Hospital Oakland Research Institute
5700 Martin Luther King Junior Way
Oakland, California 94609
Telephone: 510.450.7639
Email: jbastacky-at-chori.org
FAX: 510.450.7910



From MicroscopyL-request-at-ns.microscopy.com Thu May 5 07:43:02 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 05 May 2005 09:10:37 -0400
Subject: [Microscopy] Re: Lung EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marc
There was a group here years ago that did a lot of cryostat work on
lung, and I've been trying to remember what they did (they had their
own technician, and only came here to use my equipment). I don't
remember the details unfortunately, but I do remember that they would
fill the lungs with the freezing medium (OCT, perhaps mixed with
sucrose). The PI of the project was Spencer Danto. Perhaps a Medline
or Google search for his name would help. (Last I knew, he was in
southern CA).
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu May 5 15:33:26 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Thu, 05 May 2005 09:10:37 -0400
Subject: [Microscopy] Re: Lung EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marc
There was a group here years ago that did a lot of cryostat work on
lung, and I've been trying to remember what they did (they had their
own technician, and only came here to use my equipment). I don't
remember the details unfortunately, but I do remember that they would
fill the lungs with the freezing medium (OCT, perhaps mixed with
sucrose). The PI of the project was Spencer Danto. Perhaps a Medline
or Google search for his name would help. (Last I knew, he was in
southern CA).
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Thu May 5 21:58:04 2005



From: MicroscopyListserver :      zaluzec-at-microscopy.com
Date: Thu, 5 May 2005 21:58:05 -0500
Subject: [Microscopy] viaWWW: Technai12 Dual processor board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} Date: Thu, 5 May 2005 07:00:26 -0500
} To: Zaluzec-at-MICROSCOPY.COM
} From: coetzees-at-mopipi.ub.bw ()
} Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW:
} Status: RO
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (coetzees-at-mopipi.ub.bw) from
} http://microscopy.com/MicroscopyListserver/MLFormMail.html on
} Thursday, May 5, 2005 at 07:00:26
} ---------------------------------------------------------------------------
}
} Email: coetzees-at-mopipi.ub.bw
} Name: Stephan H Coetzee
}
} Organization: University of Botswana
}
} Title-Subject: [Microscopy] [Filtered] MListserver:
}
} Question: Urgent request. Our Technai12 Dual processor board has died. A
} } Altos 1000 P3 server. Urgently searching for a M9-GX-CPU (97506-2)
} } DUAL PROCESSOR CONTROL CARD TO FIT A M9L-LB MAIN BOARD (99712-2)
} } fei is a bit expensive. First try the list, then cough up I would guess...
} } Thanks
} }
} } Since some mail do get Lost, Bounces, etc Please send a
} } duplicate/copy of all urgent mail to:
} }
} } coetzeesh-at-yahoo.co.uk {mailto:coetzeesh-at-yahoo.co.uk}
} }
} } Mr S. H. Coetzee
} } Electron Microscope Unit
} } University of Botswana
} } Private Bag 0022
} } Gaborone
} } Botswana
} } Phone : +267 355 2462/5222
} } Mobile : +267 718 36547
} } Fax : +267 318 5097
} } e-mail : coetzees-at-mopipi.ub.bw
}
}
} ---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Thu May 5 22:26:31 2005



From: :      Colin.Veitch-at-csiro.au
Date: Fri, 6 May 2005 13:23:19 +1000
Subject: [Microscopy] Edwards E306 thanks and electron beam deposition question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

Firstly, thank you to all those who replied to my question on the E306
evaporator - I think I have it nutted out now. It didn't help that the
valve handle was out of alignment by around 45 degrees!!

Secondly, I have a question about electron beam thin film deposition. A
colleague has had some Fe films deposited on a cleaved semiconductor
surface and I was wondering what if any intermixing of the Fe and
semiconductor would occur at the interface.

I guess I need to know the energy of the Fe ions as they hit the
surface. All I know is that the electron beam energy is 5.5 kV, power
is around 500 W, the distance between crucible and target is ~45 cm (18
inches) and the vacuum is around 10E-6 torr. Is it possible to
calculate the Fe energy from this? I have no experience with this
technique at all.

Thank you very much for any help with this.

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
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From MicroscopyL-request-at-ns.microscopy.com Thu May 5 22:32:30 2005



From: cooldotcalm :      cooldotcalm-at-earthlink.net
Date: Thu, 5 May 2005 20:31:05 -0700
Subject: [Microscopy] Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gareth

The best software I've seen--particularly for the cost (FREE)--is NIH
Image. This program has been around a long time. It was written for
the Medical field by the National Institute of Health. This program is
for the Mac, however, there is a version written for PC which mimics the
"NIH Image" version. I don't recall the name of it as it has changed
recently, but if you go to the NIH website and search for "NIH Image"
you should find a reference for the PC version. Clue: the PC version
previously went by the name "Scion Image". A search of either of these
names should yield references to a downloadable, Windows compatible
program. Also, they permit macros via scripting.


Jeff Chames
Sandia National Labs
jmchame-at-sandia.gov

-----Original Message-----
} From: Gareth Morgan [mailto:Gareth.Morgan-at-labmed.ki.se]
Sent: Tuesday, May 03, 2005 5:25 AM
To: Microscopy-at-microscopy.com

Hi

We are using a 'home grown' software package for image analysis of
digital
photomicrographs. The author works here but might move soon which makes
me
think about the future.

Can anyone give me tips about good all-round image analysis software
with
standard forms of analysis - feature counts, areas etc. Preferably that
doesn't cost an arm and a leg. We are using PCs here.

Thanks

Gareth

With best wishes,

Gareth

http://www.ki.se/biomedlab
e-mail Gareth.Morgan-at-labmed.ki.se

Tel +46 8 5858 1038
Fax +46 8 5858 7730

Gareth Morgan MPhil MSc FIBMS,
Department of Histo/cytopathology, Laboratory Medicine (Labmed),
Karolinska Institute, Karolinska University Hospital at Huddinge, F46 SE
141 86 Stockholm Sweden

OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10.
Laboratoriet
för klinisk patologi och cytologi.

NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10.
Clinical Histo- and Cytopathology Laboratory.






From MicroscopyL-request-at-ns.microscopy.com Thu May 5 22:58:28 2005



From: suemosolf-at-yahoo.com (by way of Ask-A-Microscopist)
Date: Thu, 5 May 2005 22:58:10 -0500
Subject: [Microscopy] AskAMicroscopist: image blurry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (suemosolf-at-yahoo.com) from
http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Thursday, May 5, 2005 at 22:49:56
---------------------------------------------------------------------------

Email: suemosolf-at-yahoo.com
Name: Sue Mosolf

Organization: Hartnell College

Education: Undergraduate College

Location: Salinas, CA, USA

Question: When viewing a slide through a compound microscope, what
would cause the image to appear blurry and out of focus? (other than
the eyepiece being dirty)

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri May 6 09:19:36 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 06 May 2005 10:17:38 -0400
Subject: [Microscopy] Re: AskAMicroscopist: image blurry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Sue:

Anything in the light path could be causing the problem.

1. The slide could be dirty, upside down (the coverslip should be up,
toward the objective lens) or poorly prepared. Clean it and look at it
with another microscope.
2. Is the image poor with all objectives? If it is sharp with one
objective lens and blurred with another then the objective could be
dirty, loose or defective. If you think the objective is the problem
unscrew it and look at the front lens element with a magnifying glass or
the eyepiece of the 'scope turned around backwards. If the lens looks
dirty, clean it and reinstall it.
3. If the image is blurry with all objectives and the slide is not the
problem then the focusing mechanism or the inner optical path of the
microscope is probably at fault.

Geoff

by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (suemosolf-at-yahoo.com) from
} http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
} on Thursday, May 5, 2005 at 22:49:56
} ---------------------------------------------------------------------------
}
}
} Email: suemosolf-at-yahoo.com
} Name: Sue Mosolf
}
} Organization: Hartnell College
}
} Education: Undergraduate College
}
} Location: Salinas, CA, USA
}
} Question: When viewing a slide through a compound microscope, what
} would cause the image to appear blurry and out of focus? (other than
} the eyepiece being dirty)
}
} ---------------------------------------------------------------------------
}
}


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From MicroscopyL-request-at-ns.microscopy.com Fri May 6 10:48:29 2005



From: Dale Batchelor :      dale_batchelor-at-ncsu.edu
Date: Fri, 6 May 2005 11:51:32 -0400
Subject: [Microscopy] AFM Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} AFM short course is coming June 13 -17, 2005 so avoid the rush and
} register now!
}
} "AFM and Other Scanned Probe Microscopies" presented at N.C. State
} University in Raleigh, NC by Prof. Phil Russell, Dr. Joe Griffith and
} others.
} Lab sessions will utilize instrumentation from most major
} instrumentation vendors.
}
} This one-week short course has evolved from the numerous Scanned Probe
} Microscopy courses developed and taught by Prof. Russell over the past
} 2 decades. It is designed for technicians, scientists, engineers, and
} researchers. The course includes laboratories with hands-on time using
} a variety of scanning probe microscope (SPM) systems. Each student
} will receive a notebook of all materials covered in the lectures and
} animation/simulation software covering AFM principles.
}
} Register online at www.ncsu.edu/aif/afmcourse
}
Phillip E. Russell, Ph.D.
N.C. State University
Director, Analytical Instrumentation Facility
Professor, Materials Science and Engineering
e-mail: prussell-at-ncsu.edu

Dale Batchelor, Ph.D.
N.C. State University
Analytical Instrumentation Facility
EGRC room 318C
Campus Box 7531
Raleigh, NC 27695
Office 919-515-3841
FAX 919-515-6965
E-Mail dale_batchelor-at-ncsu.edu
Website www.ncsu.edu/aif



From MicroscopyL-request-at-ns.microscopy.com Fri May 6 12:34:01 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Fri, 06 May 2005 12:32:49 -0500
Subject: [Microscopy] Re: AskAMicroscopist: image blurry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Sue

Sounds like a case of spherical aberration. Sources COULD be:
a. Fingerprint on front element of objective (you may need to unscrew the objective and sit it, thread-side down) under a stereo to see it)
b. Wrong coverslip (check objective markings - if 0.17mm, should be a #1-1/2)
c. Two or more coverslips stuck together
d. Slide is wrong side up (sample should face the objectives in most cases.... I've been educated about some interesting advanced microscopy lately where that might not be the case... more later)
e. If using oil, could be old oil on the front of the objective (write me about how to clean) or could be the wrong oil. Oils are often engineered to match the dispersion characteristics of specific optics. Also, I remember some years ago trying Zeiss oil on a Nikon scope: the Nikon was not happy.
f. Check to see if it is blurry with several objectives... could be a lens element out of alignment, too.

Well, that should get you started. Let me know if you find the answer.

Good hunting!
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.


At 10:58 PM 5/5/2005, suemosolf-at-yahoo.com wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Fri May 6 14:26:49 2005



From: Carolyn Marks :      cmarks-at-richmond.edu
Date: Fri, 6 May 2005 15:20:03 -0400
Subject: [Microscopy] Vistek vibration isolation tables

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all--

I need to purchase a vibration isolation table to be used to support a
ultramicrotome. In the past I have used "air tables", all of which
require compressed air of one type or another. I also found a passive
vibration isolation system by Vistek, which does not require a
compressed air source, and the "floating" portion of the table can be
custom cut to fit the footprint of the ultramicrotome.

I know these tables are used for patch clamping, but has anyone out
there used this type of table for sectioning? I would be interested in
hearing about it's performance. Oh, one other important feature is the
mechanical room for the science center is on the other side of the
imaging suite wall....

Thanks for your comments!

Carolyn


Carolyn B. Marks, M.S.
Director of Biological Imaging
Department of Biology
Gottwald Science Center
28 Westhampton Way
University of Richmond, VA 23173

Office: (804) 484-1541
Lab: (804) 289-8775



From MicroscopyL-request-at-ns.microscopy.com Fri May 6 18:40:23 2005



From: Elizabeth R.Wright :      erwright-at-caltech.edu
Date: Fri, 6 May 2005 16:40:57 -0700
Subject: [Microscopy] Short Course in Cryo-EM Technologies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A Short Course in Cryo-HRSEM/STEM/TEM

July 13 – 15, 2005

Presented by the Integrated Microscopy & Microanalytical Facility of
Emory University in Atlanta, GA.

The course will cover conventional vitrification of molecules and thin
specimens on grids for cryo-TEM and cryo-STEM, bulk specimens will be
prepared for 3-D cryo-high resolution SEM. Chemists, biochemists, and
structural cell biologists will learn strategies for
cryo-immobilization and low temperature imaging. Participants will be
provided with bulk specimens, bacterium, and macromolecules to study.
Participants may bring their own specimens as well. Day one will
include seminars by faculty and vendors followed by two days of
practical experience with cryo-preparation and imaging.

The faculty of the course will include:
Dr. Robert P. Apkarian, director of the IM&MF and course organizer
(Emory – vast experience with all cryo-HRSEM/STEM technologies applied
to biological and chemical systems)
Dr. Elizabeth R. Wright (Caltech – cryo-TEM of proteins, cells, and
viral particles plus cryo- and cryoetch-HRSEM of protein hydrogels)
Mr. Johnny Hagen (Technotrade Internationl – high pressure freezing)
Mr. Robert Morrison (Gatan – TEM/HRSEM cryostages)
Dr. Jaap Brink (JEOL – cryo-TEM)
Mr. Dave Roberts (RMC -Boeckeler – cryo-ultramicrotomes and high
pressure freezing)
Dr. Gregory Becker (RMC-Boeckeler – cryo-ultramicrotomes and high
pressure freezing)
Ms. Linda Melanson (FEI – cryo-TEM and Vitrobot)

For more information, please contact Dr. Robert P. Apkarian.

Dr. Robert P. Apkarian, Director
Integrated Microscopy & Microanalytical Facility
Dept. of Chemistry/ Emory University
1521 Dickey Dr.
Atlanta GA 30322
http://www.electronmicroscopy.emory.edu
404 727-7766




From MicroscopyL-request-at-ns.microscopy.com Fri May 6 22:58:19 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 06 May 2005 20:57:55 -0700
Subject: [Microscopy] Iridium sputter coater material

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

Does anyone have any experiences to share about using
Ir for turbo-pumped sputter coating for fine grain
FESEM imaging?

gary g.



From MicroscopyL-request-at-ns.microscopy.com Sat May 7 14:18:34 2005



From: Microscopy Today :      microscopytoday-at-tampabay.rr.com
Date: Sat, 07 May 2005 15:17:58 -0400
Subject: [Microscopy] Microscopy Today May 2005 Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the May 2005 Microscopy Today table of contents. I will close
the subscription list for this issue on Thursday May 12, 2005.

Microscopists in North America and MSA members anywhere can subscribe
for free. Anyone else may subscribe for US$35 per year (to PARTIALLY
cover postage). All subscriptions at http://www.microscopy-today.com
Thank you.

Stephen W. Carmichael
Visualizing Gene Expression in Real-Time

Harry A. Alden et al.
“Objects Worthy of Notice” Microscopical Anatomy of Selected Plants
Collected by The Lewis & Clark Expedition

Jack Vermeulen and Heiner Jaksch
A Novel GEMINI® STEM Detector System

Kazuo Ishizuka and Brendan Allman
Phase Measurement in Electron Microscopy, Using the Transport of
Intensity Equation

Vitaly Vodyanoy
High Resolution Light Microscopy of Live Cells

U. Schmidt, et al.
Nondestructive, High-Resolution Materials Characterization with the
Confocal Raman-AFM

Jerry Sedgewick
Making Anaglyphs in Photoshop

Trisha Rice and Ralph Knowles
Ultra High Resolution SEM on Insulators and Contaminating Samples

Yuqiang Jiang, et al.
Influence of Illumination Conditions on Temperature in Sample Cell and
the Output of a Quadrant Detector in an Optical Tweezers System

D. Schichnes, J. Nemson, and S. Ruzin
Microwave Protocols for Plant and Animal--Paraffin Microtechnique

W. John Wolfgong
Raman Microscopy as an Aid in Failure Analysis – Examples From the Lab

Elaine Humphrey
Virtual Electron Microscopy for Undergraduate/Graduate Classes

New and Interesting PITTCON 2005
Industry News
NetNotes

Ron Anderson, Editor
Microscopy Today




From MicroscopyL-request-at-ns.microscopy.com Sat May 7 18:21:56 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 07 May 2005 16:21:24 -0700
Subject: [Microscopy] Iridium sputter coater material [2]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is an amplification of my prior posting.

I am looking to replace/augment my Denton Desk II
sputter coater with a unit that does finer grain
and greatly reduces hydrocarbon contamination.

The metal elements of choice seem to be Os, Au/Pd, Ir and Pt.
Os requires a special coating unit. It is pricey.
I would like to do fine grain C and fine grain metal.

My conclusion is that I can convert my Desk II to C fiber
coating and then get a turbo-pumped metal coater. This then
narrows down the units to Denton Desk IV TSC or the SVG
ion dep system. Cold coating is preferable.

Therefore, the questions on the table are (1) which metals are
best for high res FESEM (no Cr) and (2) which systems are
reliable and easy to use? The Denton Desk II has been
very good. But I am now seeing too much grain interference
and hydrocarbon contamination such that I need a higher quality
vacuum deposition system. This is a one shot deal. So
I need to be right the first time.

Any feedback out there about this or the respective systems?

gg

--------------------------------------------------------

Hi:

Does anyone have any experiences to share about using
Ir for turbo-pumped sputter coating for fine grain
FESEM imaging?

gary g.



From MicroscopyL-request-at-ns.microscopy.com Sun May 8 19:38:45 2005



From: Ivan Petrov :      petrov-at-mrl.uiuc.edu
Date: Sun, 8 May 2005 19:41:38 -0500
Subject: [Microscopy] MMMS meeting in Urbana, June 9-10, 2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Center for Microanalysis of Materials cordially invites you to the 2005 meeting of the Midwest Microscopy and Microanalysis Society, June 9-10 in Urbana. The topic of the meeting is



“Dynamics of Materials Revealed by Electron Microscopyâ€



Information can be found at:



http://cmm.mrl.uiuc.edu/MMMS05/index.htm {http://cmm.mrl.uiuc.edu/MMMS05/index.htm}




We expect an excellent regional meeting where leading MMMS microscopists will present their best science. We have outstanding visitors as well. See a list of invited lectures at:



http://cmm.mrl.uiuc.edu/MMMS05/Invited.htm {http://cmm.mrl.uiuc.edu/MMMS05/Invited.htm}



We encourage students to participate in poster presentations in the general area of electron microscopy and microanalysis. Two student poster awards will be given to registered student participants.



The meeting is sponsored by:



FEI, Fischione Instruments, Gatan, JEOL, Hitachi, MMMS, and CMM.



Looking forward to welcoming you in Urbana.



Ivan Petrov




From MicroscopyL-request-at-ns.microscopy.com Mon May 9 10:50:59 2005



From: James Pawley :      jbpawley-at-wisc.edu
Date: Mon, 09 May 2005 10:53:42 -0500
Subject: [Microscopy] Re: Iridium sputter coater material [2]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Ion-beam sputtered Pt, gets my vote.

Jim P.
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU
"A scientist is not one who can answer questions but one who can
question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39


From MicroscopyL-request-at-ns.microscopy.com Mon May 9 13:14:38 2005



From: Becky Holdford :      r-holdford-at-ti.com
Date: Mon, 09 May 2005 13:16:09 -0500
Subject: [Microscopy] Re: Iridium sputter coater material [2]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary: have you looked at Emitech (http://www.empdirect.com/k575.html)?
They offer turbo-pumped Ir coaters which I use almost daily. Another
alternative is South Bay Technology's IBS/e ion beam sputter coater. I
use an older model of that one and it produces an excellent coating. No
financial interest in either company, just a satisfied customer.

Gary Gaugler wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} This is an amplification of my prior posting.
}
} I am looking to replace/augment my Denton Desk II
} sputter coater with a unit that does finer grain
} and greatly reduces hydrocarbon contamination.
}
} The metal elements of choice seem to be Os, Au/Pd, Ir and Pt.
} Os requires a special coating unit. It is pricey.
} I would like to do fine grain C and fine grain metal.
}
} My conclusion is that I can convert my Desk II to C fiber
} coating and then get a turbo-pumped metal coater. This then
} narrows down the units to Denton Desk IV TSC or the SVG
} ion dep system. Cold coating is preferable.
}
} Therefore, the questions on the table are (1) which metals are
} best for high res FESEM (no Cr) and (2) which systems are
} reliable and easy to use? The Denton Desk II has been
} very good. But I am now seeing too much grain interference
} and hydrocarbon contamination such that I need a higher quality
} vacuum deposition system. This is a one shot deal. So
} I need to be right the first time.
}
} Any feedback out there about this or the respective systems?
}
} gg
}
} --------------------------------------------------------
}
} Hi:
}
} Does anyone have any experiences to share about using
} Ir for turbo-pumped sputter coating for fine grain
} FESEM imaging?
}
} gary g.
}
}

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From MicroscopyL-request-at-ns.microscopy.com Mon May 9 17:38:51 2005



From: camiller-at-anatomy.iupui.edu (by way of MicroscopyListserver)
Date: Mon, 9 May 2005 17:41:50 -0500
Subject: [Microscopy] viaWWW: First Annual Meeting of the Indiana Microscopy Society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (camiller-at-anatomy.iupui.edu) from http://www.msa.microscopy.com/MLFormMail.html on Monday, May 9, 2005 at 13:07:22
---------------------------------------------------------------------------

Email: camiller-at-anatomy.iupui.edu
Name: Caroline Miller

Organization: Indiana University

Title-Subject: [Microscopy] [Filtered] First Annual Meeting of the Indiana Microscopy Society

Question: The newly formed Indiana Microscopy Society, welcomes all microscopists to our first annual Spring Meeing. It will be held all day on Friday, May 20th, 2005, at the Van Nuys Medical Science Building on the IUPUI campus in Indianapolis. The meeting will be hosted by the Electron Microscopy Center, Indiana University School of Medicine. We have two speakers planned. Kent McDonald will talk about high pressure freezing and Robert Bacallao about confocal microscopy. There will also be tours of the EM Center and Indiana Center for Biological Microscopy. Please check the web site for detailed information. www.indianamicroscopy.org

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon May 9 17:39:21 2005



From: varble.4-at-uky.edu (by way of MicroscopyListserver)
Date: Mon, 9 May 2005 17:42:21 -0500
Subject: [Microscopy] viaWWW: Sarcomere length with light and electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (varble.4-at-uky.edu) from http://www.microscopy.com/MLFormMail.html on Monday, May 9, 2005 at 15:17:56
---------------------------------------------------------------------------

Email: varble.4-at-uky.edu
Name: Jaime Varble

Organization: Graduate Student University of Kentucky Meat Science

Title-Subject: [Microscopy] [Filtered] MListserver:Sarcomere length with light and electron microscopy

Question: I am interested in measuring sarcomere lengths of muscle from beef cattle. I am looking at the longissimus dorsi, semitendinosus and semimembranosus muscles. These muscles have been stored in a -80 freezer for about a month. I have a few questions:1)does anyone have experiance with working with samples that have been frozen at this temperature, and was there an affect on sarcomeres 2)where to find procedures for both light and electron microscopy and 3)any suggestions on the best method (phase contrast, SEM, TEM...)to use to measure sarcomeres. Any suggestions would be greatly appreciated. thank You

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon May 9 17:41:01 2005



From: john-rong-at-ouhsc.edu (by way of Ask-A-Microscopist)
Date: Mon, 9 May 2005 17:44:01 -0500
Subject: [Microscopy] AskAMicroscopist: help a college student for his project

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (john-rong-at-ouhsc.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, May 9, 2005 at 14:12:12
---------------------------------------------------------------------------

Email: john-rong-at-ouhsc.edu
Name: John Rong

Organization: University of Oklahoma Health Sciences Center

Education: Graduate College

Location: Oklahoma City, Oklahoma

Question: I was asked to help a college student for his project. I am a professor but unfortunately I have no knowledge about this topic. I would appreciate any help you can provide so that I can pass the information to the student. Thanks!

The student plans to observe the surface structures of a vascular stent that is implanted inside a pigís coronary artery. The sample has been stored in ìformalinî (10% of formaldehyde) for more than 6 months. The microscope is an Environmental Scanning Electron Microscope.

This student would like to learn the protocols and procedures for preparing his sample. For properly operating the microscope, what special procedures or steps have to be followed and necessary operating conditions such as temperature, ATM, humidity, etc. to be maintained?

Thanks again.


---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Mon May 9 19:04:56 2005



From: Elaine Humphrey :      ech-at-interchange.ubc.ca
Date: Mon, 09 May 2005 17:07:34 -0700
Subject: [Microscopy] Re: Iridium sputter coater material [2]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gary
If you look at our website, we put up some images that were given to
us by Emitech of different coatings. It might be useful to you.
http://www.emlab.ubc.ca/sputcomp.htm
Elaine


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca


From MicroscopyL-request-at-ns.microscopy.com Mon May 9 19:16:04 2005



From: Tom Murray :      murraytm-at-u.washington.edu
Date: Mon, 9 May 2005 17:18:16 -0700
Subject: [Microscopy] EBSD Sample Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a student who would like to examine a metal sample using EBSD.
This sample has to be mounted on a 12.5 mm stub (JEOL JSM 7000 SEM) for
this technique. It alspo has to be polished. What is the best way to
polish these small samples without embedding them in the traditional
metallographic way?

Thanks,

Tom

------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email: murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195



From MicroscopyL-request-at-ns.microscopy.com Mon May 9 20:48:48 2005



From: cooldotcalm :      cooldotcalm-at-earthlink.net
Date: Mon, 9 May 2005 18:51:17 -0700
Subject: [Microscopy] EBSD Sample Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom,

If you need to analyze to the edge, you need to pot the specimen and (if
necessary) cut the epoxy away afterwards to a managable size. Polishing
an unmounted specimen--which our Metallographer does often--leaves the
edges rounded and impossible to obtain good images or EBSD patterns
from. He holds the specimen with double sided tape to polish and has
good success. But I imagine the flatter the specimen, the better, in
order to minimize twisting in a shear manner during the polishing.

Jeff Chames
Sandia National Labs
jmchame-at-sandia.gov

-----Original Message-----
} From: Tom Murray [mailto:murraytm-at-u.washington.edu]
Sent: Monday, May 09, 2005 4:18 PM
To: {Microscopy-at-msa.microscopy.com}

I have a student who would like to examine a metal sample using EBSD.
This sample has to be mounted on a 12.5 mm stub (JEOL JSM 7000 SEM) for

this technique. It alspo has to be polished. What is the best way to
polish these small samples without embedding them in the traditional
metallographic way?

Thanks,

Tom

------------------------------------------------------------------------

---------------------------------------------
Thomas M Murray email:
murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax:
(206)543-3100
Box 352120 302 Roberts Hall Cell:
(425)345-0083
University of Washington
Seattle, WA 98195




From MicroscopyL-request-at-ns.microscopy.com Mon May 9 22:49:01 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 09 May 2005 20:48:53 -0700
Subject: [Microscopy] Re: EBSD Sample Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How thick is the film?

My usual way is to take a 12mm square chunk and
polish it down to 0.02u coloidal and then ground it
with Pella colloidal silver dag...or whatever type
you prefer or like or have.

Then, tilt to 70 degrees and go for it.

However, you do not state what element you are looking at.
This can make a big deal. If the grains are large, fine.
If the grains are small, you will get problems with
lattice interaction. Net result--garbage. In this
respect, KV and probe size and probe current make a
big impact. If small grains, one cannot tell the
differences between lattices. The software can't.

So, what metal are you working with?

gary g.


At 05:18 PM 5/9/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From MicroscopyL-request-at-ns.microscopy.com Tue May 10 08:41:14 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 10 May 2005 09:43:06 -0400
Subject: [Microscopy] Re: viaWWW: Sarcomere length with light and electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Jaime,
Its always best to start an EM project with fresh samples. Since you
don't have that option:
Do you know what, if anything was done to the muscle before it was
frozen? IE: was it treated in any way to lessen or prevent ice
crystal damage? How was it frozen? Was it just put into the -80, or
was it flash-frozen in liquid nitrogen? When the pieces of muscle
were isolated, were they pinned or tied down in anyway to try to keep
the relaxed muscle length? For that matter, was the muscle treated
with a relaxing buffer to prevent contraction?
All that being said, I doubt that you will get much in the way of
beautiful EM images from tissue that was frozen and stored, but if
you cant do a freeze-substitution fixation followed by embedding and
sectioning, you may see enough to make measurements. I would expect
that the muscle fibers will appear highly contracted: you will see
Z-lines and I-bands (thick filaments), but not much in the way of
A-bands (thin filaments) or M-lines. With highly contracted muscle,
it will be difficult if not impossible to get a measurement of
relaxed sarcomere length.
As for looking at the muscle in LM, if you can get cryosections cut
at 5-10 micrometers thick and oriented so that you have longitudinal
sections of the muscle, you will be able to see things quite nicely
in either phase contrast or DIC . DIC, if you have it may be better,
since muscle is highly birefringent, and if the orientation is good,
the banding shows up quite well.
You may write to me off-list fi you have futher questions.
Good luck,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue May 10 10:26:28 2005



From: Tom Murray :      murraytm-at-u.washington.edu
Date: Tue, 10 May 2005 08:28:43 -0700
Subject: [Microscopy] Re: EBSD Sample Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

Thanks for the suggestions. I'll have to discuss with the student
exactly what metal he is looking at. The suggestion of using a jig
reminded me that I do have a tripod polisher which I I should be able
to use with no angle.

Tom

------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email: murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195



From MicroscopyL-request-at-ns.microscopy.com Tue May 10 11:22:23 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 10 May 2005 11:24:54 -0500
Subject: [Microscopy] Decon 90

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Does anyone know of a North American supplier for the laboratory
cleanser Decon 90? Or is there a good substitute for this? It's used
as a glassware cleanser and radiation decontaminator.

Thanks,
Randy

P.S. Dear Homeland Security person: We are not using it for the latter
purpose.

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








From MicroscopyL-request-at-ns.microscopy.com Tue May 10 11:48:56 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 10 May 2005 11:51:29 -0500
Subject: [Microscopy] Decon 90 again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I thought I had exhausted all my options at finding a substitute for
this lab cleaner, but after one last try I discovered that Contrad 70
seems to be the same, or nearly the same, product as Decon 90. It is
available through north American suppliers, including Fisher, if anyone
else needs this information.

Thanks to those who answered or were planning to!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








From MicroscopyL-request-at-ns.microscopy.com Tue May 10 13:24:06 2005



From: David Henriks :      henriks-at-southbaytech.com
Date: Tue, 10 May 2005 11:26:14 -0700
Subject: [Microscopy] Re: Re: EBSD Sample Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom:

South Bay Technology does have some applications notes on EBSD sample
preparation that may be useful. One paper, presented at UC Irvine
titled "Mechanical Polishing Methods of Metal Samples for EBSD" may be
of particular interest. We also have an application note (#71) titled
"Improving Surface Quality of
Petrographic Sections for EBSD" . I would be pleased to send you these
papers in PDF format if you think they would be useful.

Of course, I have a vested interest in promtoting this as we also offer
a complete EBSD Sample Preparation System as well!

Best regards-

David

--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.


Tom Murray wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Hi All,
}
} Thanks for the suggestions. I'll have to discuss with the student
} exactly what metal he is looking at. The suggestion of using a jig
} reminded me that I do have a tripod polisher which I I should be able
} to use with no angle.
}
} Tom
}
} ------------------------------------------------------------------------
} ---------------------------------------------
} Thomas M Murray email:
} murraytm-at-u.washington.edu
} Electron Microscopy Center Manager Phone: (206)543-2836
} Materials Science & Engineering Fax: (206)543-3100
} Box 352120 302 Roberts Hall Cell: (425)345-0083
} University of Washington
} Seattle, WA 98195
}
}
}





From MicroscopyL-request-at-ns.microscopy.com Tue May 10 15:11:06 2005



From: Gordon Couger :      gcc-at-couger.com
Date: Tue, 10 May 2005 15:12:35 -0500
Subject: [Microscopy] Re: Re: viaWWW: Sarcomere length with light and electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Unless this muscle tissue is special for some reason you should
be able to get it a fresh as possible on the kill floor of a
meat packing plant. I believe that there is one on campus at the
college where the the post oriented. They will be used to this
kind of request.

At lest you can get some for comparison to your samples to see
how much freeze damage there is.

Gordon Couger
Biosystems& Ag Engineering (retired)
Oklahoma State University
www.couger.com/gcouger


Leona Cohen-Gould wrote:
}

}
}
} Dear Jaime,
} Its always best to start an EM project with fresh samples.
Since you
} don't have that option:
} Do you know what, if anything was done to the muscle before
it was
} frozen? IE: was it treated in any way to lessen or prevent
ice crystal
} damage? How was it frozen? Was it just put into the -80, or
was it
} flash-frozen in liquid nitrogen? When the pieces of muscle were
} isolated, were they pinned or tied down in anyway to try to
keep the
} relaxed muscle length? For that matter, was the muscle
treated with a
} relaxing buffer to prevent contraction?
} All that being said, I doubt that you will get much in the
way of
} beautiful EM images from tissue that was frozen and stored,
but if you
} cant do a freeze-substitution fixation followed by embedding and
} sectioning, you may see enough to make measurements. I would
expect
} that the muscle fibers will appear highly contracted: you
will see
} Z-lines and I-bands (thick filaments), but not much in the
way of
} A-bands (thin filaments) or M-lines. With highly contracted
muscle, it
} will be difficult if not impossible to get a measurement of
relaxed
} sarcomere length.
} As for looking at the muscle in LM, if you can get
cryosections cut at
} 5-10 micrometers thick and oriented so that you have
longitudinal
} sections of the muscle, you will be able to see things quite
nicely in
} either phase contrast or DIC . DIC, if you have it may be
better, since
} muscle is highly birefringent, and if the orientation is
good, the
} banding shows up quite well.
} You may write to me off-list fi you have futher questions.
} Good luck,
} Lee




From MicroscopyL-request-at-ns.microscopy.com Tue May 10 16:09:59 2005



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 10 May 2005 16:12:13 -0500
Subject: [Microscopy] Kai Chien email address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am trying to locate Kai Chien's email address. After searching the
MSA membership list and conducting other searches on the web, I am
asking this group if anyone has his address.

Many thanks.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################


From MicroscopyL-request-at-ns.microscopy.com Tue May 10 17:14:22 2005



From: Karen Weidenheim :      peterwimsey-at-msn.com
Date: Tue, 10 May 2005 18:15:34 -0400
Subject: [Microscopy] RE: Re: Re: viaWWW: Sarcomere length with light and electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jaime, the procedures you need to follow on the fresh muscle tissue are
those used for muscle biopsies in human patients, as Leona has outlined. A
good reference is Chapter 6 in Advanced laboratory methods in histology and
pathology, Ulrika Mikel, editor, Armed forces Institute of pathology,
American Registry of Pathology, Washington DC. 1994. You should take a
piece for electron microscopy, making sure it is orientable in the
longitudinal plane so you can see the entire length of the sarcomere (from
z-band to z-band). You can either put fresh longitudinally oriented tissue
in EM fixative or you can pin out the specimen, fix it, then dissect it. I
cut my EM muscle specimens to be longer than they are wide so they are
orientable when stained with osmium.
I would not bother with the previously frozen tissue. I would go get my
own, as fresh as possible.
Karen




Karen M. Weidenheim, M.D.
Professor of Pathology, Clinical Neurology and Clinical Neurosurgery
Director, Division of Neuropathology
Montefiore Medical Center
111 East 210th Street
Bronx, NY 10467
(718) 920-4446
FAX (718) 653-3409




} From: Gordon Couger {gcc-at-couger.com}
} To: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
} CC: by way of MicroscopyListserver {varble.4-at-uky.edu} ,
} microscopy-at-microscopy.com
} Subject: [Microscopy] Re: Re: viaWWW: Sarcomere length with light and
} electron microscopy
} Date: Tue, 10 May 2005 15:12:35 -0500
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue May 10 21:39:08 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Tue, 10 May 2005 21:42:10 -0500
Subject: [Microscopy] Administrivia: System Testing Please Ignore -- Nestor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

I'm doing a system update and need to run a full distribution test
to the entire list to confirm operation.

PLEASE IGNORE THIS MESSAGE AND DO NOT REPLY,
I WILL KNOW IF THERE IS A PROBLEM.


Nestor
Your Friendly Neighborhood SysOp
9:42 PM -MAY 10, 2005


From MicroscopyL-request-at-ns.microscopy.com Tue May 10 23:03:43 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 10 May 2005 17:58:09 -0700
Subject: [Microscopy] Sputter coater summary--closure?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers:

I have been trying to find a new coater replacement
for my Denton Desk II for fine grain metal. I thought
that this would be an easy and straightforward task.
Wrong.

As it seems to be now, converting the Denton Desk II
to C fiber is a good move. However, the metal replacement
option unit is very difficult. Given the price points and
the technical aspects of each vendor's offering, it is
tough to decide--when I am allowed one irrevocable decision.

Well, given that M&M 2005 is coming up...this may be the
salvation for my dilemma. I will bring identical specimens
and have them treated by each of the vendors. Then, I can
hopefully get these imaged by Zeiss smt. That will narrow
down the prospects.

In this respect, are there any subtleties that I should
consider for this one-shot comparison? I.e., I should
eliminate all variables that would compromise a flat field
comparison. Imaging will be at 100KX, 200KX and 300KX.
With Zeiss, tilt angle is a negative factor. Their in-lens
detector does not work well at high tilt angles. That is OK.
I am just looking for direct comparison from one coating
method/supplier to another.

gary g.



From MicroscopyL-request-at-ns.microscopy.com Wed May 11 13:17:10 2005



From: Eric Anderson :      andersone1-at-southernct.edu
Date: Wed, 11 May 2005 14:17:56 -0400
Subject: [Microscopy] Need a <$8000 Carbon Coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings all,

We're looking to purchase a carbon-coater to prep samples for SEM, but
need to keep the cost under $8000. Any recommendations as far as
brands/vendors? We like the EMS-450, but just wanted to consider options
before buying.
Any machines in this price range that you've been happy with?

Many thanks!
~Eric

Eric Anderson
Southern CT State University
Physics Department
501 Crescent Street, JE108B
New Haven, CT 06515
203-392-6468
fax 203-392-6466



From MicroscopyL-request-at-ns.microscopy.com Wed May 11 13:27:47 2005



From: MicroscopyListserver :      zaluzec-at-microscopy.com
Date: Wed, 11 May 2005 13:29:38 -0500
Subject: [Microscopy] viaWWW: Full-Time Assistant Scientist - Center for A dvanced

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Date: Wed, 11 May 2005 13:18:47 -0500
} To: microscopy-at-microscopy.com
} From: "Ehrlich, Tracy" {tehrlich-at-mail.as.miami.edu} (by way of MicroscopyListserver)
} Subject: [Microscopy] viaWWW: Full-Time Assistant Scientist - Center for A dvanced Microscopy
} Cc:

}
}
}
} Full-Time Assistant Scientist - Center for Advanced Microscopy - Department of Chemistry
}
}
} University of Miami, Electron Microscopy Specialist: The Department of Chemistry is seeking applicants for the position of Assistant Scientist and Manager of the Department's Advanced Microscopy Laboratory, beginning June 1, 2005. Ph.D. in a suitable scientific field and prior research experience required as well as established expertise in operating TEM, SEM, ESEM, and EDS equipment. This lab provides services for the department as well as other areas of the University on a fee-for-service basis. Appointee will be responsible for managing this lab to include recommending policies & procedures, developing & administering the budget (revenues & expenses), training & supervising other individuals who may operate the equipment, insuring that equipment is properly maintained & serviced, and overseeing all aspects of the fee-for-service operation. As an assistant scientist, the individual is expected to be actively involved in the research process, either in collaboration with other faculty or independently, to include, the identification of new revenue sources, assisting in the writing of proposal, reports and publications as well as actually conducting experiments & running samples. Applicants should send a CV and arrange to have three letters of recommendation sent to Dr. V. Ramamurthy, Professor and Chair, Department of Chemistry, PO Box 249118, Coral Gables, FL 33124-0431 (murthy1-at-miami.edu). Review of applications will begin immediately and will proceed until the position is filled. Information on the department can be found at www.miami.edu/chm. University of Miami is an equal opportunity affirmative action employer.
}
}
}
}
}
}
} Ms. Tracy Ehrlich
}
} new email: {mailto:tehrlich-at-miami.edu} tehrlich-at-miami.edu
} (formerly Tracy Helenbrook)
}
} Assistant to the Vice Deans
} College of Arts & Sciences
} University of Miami
} P.O. Box 248004
} (Ashe Building, Room 227)
} Coral Gables, Florida 33124-4620
} ph. 305-284-4021
} fax 305-284-5637
}
} Acting Assistant Manager
} Department of Chemistry
} College of Arts & Sciences
} University of Miami
} PO Box 249118
} (Cox Science Center, Rm. 315)
} Coral Gables, FL 33124-0431
} ph: 305-284-1853
} fax: 305-284-4571
}
} Resident Artist
} Art Glass Program
} Department of Art & Art History
} College of Arts & Sciences
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Wed May 11 16:29:53 2005



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 11 May 2005 16:31:17 -0500
Subject: [Microscopy] TEM of hard tissues, author needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A colleague is working on an EM Methods and Protocols book in the
Methods in Molecular Biology series and needs an author for a TEM
chapter dealing with both conventional and microwave rapid
preparation procedures for hard tissues. This would deal with how to
process specimens such as bone, teeth and possibly even botanical
materials that are very hard. If you are interested (or know of
someone who has this expertise) please let me know and I will forward
the information.
Thank you.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From MicroscopyL-request-at-ns.microscopy.com Wed May 11 17:58:05 2005



From: cdjensen :      cdjensen-at-engr.colostate.edu
Date: Wed, 11 May 2005 16:56:48 -0600
Subject: [Microscopy] SEM-New User, E. coli Immobilized in Hydrogel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Trying to most accurately image the surface and interior of a bacterial
cell/hydrogel matrix. My first attemp involved keeping the hydrogel in water
up to the point of a gold sputter coating. There was obvious charging taking
place on the sample while the electron beam was turned on. I think this can
be illiminated by making the sample conductive by applying a conductive
material to the sample and sample holder.

Regards,
Cory Jensen



From MicroscopyL-request-at-ns.microscopy.com Wed May 11 22:24:10 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 11 May 2005 23:22:53 -0500
Subject: [Microscopy] Carbon coaters under $8000.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Eric Anderson wrote:
===================================================
We're looking to purchase a carbon-coater to prep samples for SEM, but
need to keep the cost under $8000. Any recommendations as far as
brands/vendors? We like the EMS-450, but just wanted to consider options
before buying.
Any machines in this price range that you've been happy with?
===================================================
See URL
http://www.2spi.com/catalog/instruments/sputter1.shtml
Option #1 for the SPI Module Carbon Coater, including the pump, the price is
$7413.62.

It has some features not normally found on other brands of coaters and these
are described on the above website page. It is 100% made in the USA and I
make that point because with the exchange rates being as they are, and the
US dollar being so low relative to European currencies, products made in
the US tend to be lower in price and in some cases, much lower in price than
imported equivalents.

So far as I know, our worldwide customer base seems to be very happy with
the quality of the carbon coating that is put down. I presume that if anyone
out there was not happy, we will hear from them after this posting.

Disclaimer: SPI Supplies manufactures the SPI Module line of sputter and
carbon coaters.

Chuck

PS: Please remember that we are nearly 100% paperless and we would ask
that any reply to this message be by way of the "reply" feature on your
software, so that the entire string of correspondence can come back to us
and all be in one place.

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Thu May 12 07:50:09 2005



From: Karl Hagglund :      hagglundk1-at-nku.edu
Date: Thu, 12 May 2005 08:48:58 -0400
Subject: [Microscopy] Used SEM value Zeiss DSM 960A

Contents Retrieved from Microscopy Listserver Archives
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Our program recently obtained a used Zeiss DSM 960A microscope (1993).
We are trying to determine everything we can about the value of the
instrument for insurance purposes. I called Zeiss, and was told this
sold for $22,000 in 1993. I know we have seen inflation since 1993, but
have trouble believing that one of these was selling for such a low
price. Can somebody out there provide me with an estimate of either the
current value or approximate retail of one these in 1993? Thanks in
advance.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu




From MicroscopyL-request-at-ns.microscopy.com Thu May 12 07:58:11 2005



From: Al Harmon :      aharmon-at-mvainc.com
Date: Thu, 12 May 2005 08:59:19 -0400
Subject: [Microscopy] TEM calabration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Looking for an economical and effective way to accurately calibrate the
magnification of a TEM at magnifications above 300,000 X. We have owned
two Mag*I*Cal's but neither have survived frequent use in the TEM and
the center has fallen out. Would appreciate any thoughts on the matter.

Al Harmon
MVA Scientific Consultants, Inc.
"aharmon-at-mvainc.com"



From MicroscopyL-request-at-ns.microscopy.com Thu May 12 08:10:04 2005



From: Gerd Leitinger :      gerd.leitinger-at-meduni-graz.at
Date: Thu, 12 May 2005 15:06:50 +0200
Subject: [Microscopy] Ultrostainer

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

We have a Leica Ultrostainer that we are now using for staining thin
sections with lead citrate and uranyl acetate. Does anybody know
whether other companies produce a similar stainer, and what is your
experience with either stainer?
I am asking because I have been asked to give advice on the
furnishing of a new electron microscopy lab. We had teething trouble
with our stainer- we had the tubing exchanged and have been advised
on several rinses in HNo3 after each stain, so it now works ok.

thank you!


Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at




From MicroscopyL-request-at-ns.microscopy.com Thu May 12 08:54:43 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Thu, 12 May 2005 08:53:26 -0500
Subject: [Microscopy] Re: SEM-New User, E. coli Immobilized in Hydrogel

Contents Retrieved from Microscopy Listserver Archives
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Cory,

?
How and in what kind of SEM were you trying to image the sample? And
what kind of sputter coater did you use?
The only way to get the real structure of hydrated hydrogels is with
cryoSEM and cryocoating for the sputter coating.

Phil

} Trying to most accurately image the surface and interior of a bacterial
} cell/hydrogel matrix. My first attemp involved keeping the hydrogel in water
} up to the point of a gold sputter coating. There was obvious charging taking
} place on the sample while the electron beam was turned on. I think this can
} be illiminated by making the sample conductive by applying a conductive
} material to the sample and sample holder.
}
} Regards,
} Cory Jensen

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/albrecht_web/Programs/microscopy/home.html


From MicroscopyL-request-at-ns.microscopy.com Thu May 12 12:13:22 2005



From: Sandeep Kohli :      skohli-at-lamar.colostate.edu
Date: Thu, 12 May 2005 11:11:49 -0600
Subject: [Microscopy] EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
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Dear Al,
The old standard was a thin gold single crystal, with lattice spacings of
0.102, 0.143 and 0.204 nanometers, or graphitized carbon, with a 0.34 nm
lattice. Both are available from EM suppliers.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "Al Harmon" {aharmon-at-mvainc.com}
To: {Microscopy-at-msa.microscopy.com}
Sent: Thursday, May 12, 2005 5:59 AM

Hi All:

I am a new entrant in this mailing list. I am trying to work with EDS
for the quantitative analysis. While reading the book by Goldstein etal,
issue related to the energy calibration of EDS came up. I was wondering
how one goes about calibrating the EDS and would appreciate if some body
could share their experience. We have Thermo EDS.

Thanks.

Sandeep Kohli

-----------------------------------------
Dr. Sandeep Kohli
Department of Chemistry
Colorado State University,
Fort Collins CO-80523
Phone: (970) 491-4076
FAX: (970) 491-1801
Email: sandeep.kohli-at-colostate.edu
Web: http://www.chm.colostate.edu/skohli




From MicroscopyL-request-at-ns.microscopy.com Thu May 12 12:14:30 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Thu, 12 May 2005 12:13:00 -0500
Subject: [Microscopy] Re: SEM-New User, E. coli Immobilized in Hydrogel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Cory

How big are the bacteria? If I could suggest an easier alternative: if your samples will travel, send them to Aetos Technologies and have them try imaging with their new CytoViva. When I visited their labs, they were routinely imaging bacteria, without any sample prep, and could observe them while living. Might be an interesting alternative.

Their tech apps specialist is Tom Hasling (334-749-0134). Give him a call and see what he thinks.

Hope this is helpful

Thanks,
Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). For details, call us at 972-943-8011 and ask for Ken Piel.

At 05:56 PM 5/11/2005, cdjensen wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Thu May 12 12:59:12 2005



From: Thomas, Frank :      FThomas-at-nrcan.gc.ca
Date: Thu, 12 May 2005 15:52:01 -0300
Subject: [Microscopy] EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Why not use the graphitised carbon specimen with a 3.44A spacing? A very
tough specimen but fairly easy to resolve with any modern TEM. Simply look
for the crystalline appearance in the swirls, shoot for a little underfocus
(narrow white fringe) and you should be OK. Its probably best to view at
500,000X plus the binocular to pick out the lattice on the screen and then
lower the magnification to the level that you require. Provide the
instrument that you are using does not step the diffraction lens between the
higher and lower levels the original focus will be fine.

I am sure any one of your well known EM accessory suppliers could help you
out?

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
} From: "Al Harmon" {aharmon-at-mvainc.com}
To: {Microscopy-at-msa.microscopy.com}
Sent: Thursday, May 12, 2005 1:59 PM

Sandeep -

About once a month or so I calibrate our older Noran Voyager EDS
system by putting the beam on a piece of nice clean copper and acquiring a
spectrum. The major Cu peak (I think it's the La1) should be at 8.048 KeV on
the energy scale. Our system has built-in software to run a FWHM test also,
which tests the resolution of the detector. Your software may also have such
a routine - consult your manual. If it turns out that your peak is not at
8.048 (+/- a wee bit), you may have to adjust the peak location using the
hardware - I don't know about your setup, but in ours there's a small screw
or knob on one of the printed circuit boards of the controlling computer
which has to be adjusted slightly to move the peak back and forth ( I think
it's what they call a potentiometer). I haven't had to do this since our
detector was rebuilt about 7 years ago, so my memory might be a bit off. You
can use other elements for your calibration, it doesn't have to be copper,
or pure, either, for that matter - as long as you're sure which peak is
which.
Of course, the good folks at Thermo should also be able to help you
out.

Frank

F.C. Thomas
FThomas-at-NRCan.gc.ca, 902-426-4635, facsimile 902-426-6152
GSC Atlantic
Natural Resources Canada, Bedford Institute of Oceanography,
P.O. Box 1006, Dartmouth, Nova Scotia B2Y 4A2
Ressources naturelles Canada, l'Institut Oceanographique du Bedford, B.P.
1006, Dartmouth, (Nouvelle-Ecosse)
B2Y 4A2
Government of Canada/Gouvernement du Canada


-----Original Message-----
} From: Sandeep Kohli [mailto:skohli-at-lamar.colostate.edu]
Sent: Thursday, May 12, 2005 2:12 PM
To: microscopy-at-microscopy.com

Hi All:

I am a new entrant in this mailing list. I am trying to work with EDS
for the quantitative analysis. While reading the book by Goldstein etal,
issue related to the energy calibration of EDS came up. I was wondering
how one goes about calibrating the EDS and would appreciate if some body
could share their experience. We have Thermo EDS.

Thanks.

Sandeep Kohli

-----------------------------------------
Dr. Sandeep Kohli
Department of Chemistry
Colorado State University,
Fort Collins CO-80523
Phone: (970) 491-4076
FAX: (970) 491-1801
Email: sandeep.kohli-at-colostate.edu
Web: http://www.chm.colostate.edu/skohli




From MicroscopyL-request-at-ns.microscopy.com Thu May 12 14:27:31 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Thu, 12 May 2005 12:24:43 -0700
Subject: [Microscopy] Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On May 12, 2005, at 10:11 AM, Sandeep Kohli wrote:

} I am a new entrant in this mailing list. I am trying to work with EDS
} for the quantitative analysis. While reading the book by Goldstein
} etal,
} issue related to the energy calibration of EDS came up. I was wondering
} how one goes about calibrating the EDS and would appreciate if some
} body
} could share their experience. We have Thermo EDS.
}
Dear Sandeep,
For energy calibration of a TN2000--a likely ancestor of your
system--we used a grid that would give a signal from Al at 1.4 keV and
from Cu at ~8.5 keV. I don't remember the exact values, but the
important point is to have two peaks near the endpoints of the range
you want to use. the simplest way to obtain such a grid is to
evaporate Al onto a Cu grid that has a carbon or formvar support and to
illuminate an area near a grid bar. I expect that your multichannel
analyzer has a routine for calibration; ours took continuous spectra
and showed how close the two possible adjustments were by displaying a
number of either { or } symbols. One then turned a set screw
corresponding to the adjustment that was the further off until there
were few symbols, then turned the other set screw, and so forth until
both adjustments were as good as possible. The two adjustments are the
position of zero energy and scale of eV/channel, so, if both energies
are above or below their proper position, the zero adjustment would be
off, and if they were one above and one below, the scale would be off.
Typically, it took about 10 minutes to do the calibration if the
energies were pretty far off, and less time if they were close. Good
luck
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Thu May 12 15:13:57 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 12 May 2005 13:12:26 -0700
Subject: [Microscopy] Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
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Yes...EDS calibration is an interesting area and probably
is done differently by different users and for different
systems. Notwithstanding, I can relay some info on what
seems to work for me.

First off, you need to identify if you have a light element
detector. This is one that will go down to B and possibly Be.
Some will not go lower than F, O or N. And I'm only talking
about window detectors. My produce with EDAX Genesis and
their DPP-2 digital pulse processor, GUI and Cryospec detector
is as follows:

1. Insert X Checker Extra (available from several suppliers and
usually will get you a free Flight Simulator [EDS variety]).

2. Set KV to 20KV and find the Cu tab on the X Checker. Follow
the instructions with X Checker and calibrate the Cu K alpha
peak (8.040KeV and the and Al K alpha peak (1.486KeV). Genesis
does this iteratively automatically when commanded.

3. Do #2 for all filter times. Adjust spot size or condenser
setting or aperture size to keep DT {35%.

4. Reduce KV to 5KV and find the F specimen. Counts will
be low. Use only the longest and next longest filter times.
Collect spectra and find where the F peak is. It should be
at .677eV. If not, adjust the energy value in the software
to bring it to that value. Genesis has HPD feature that says
if a detected element peak is off the elemental energy table
values for all shells.

5. Do the same for BN specimen.

6. Do the same for C specimen.

7. If you want, you can do Mn at 20KV and compute the FWHM value.
Frankly, I prefer the resolution figure from step #2. Mine
works out to 130.5eV at 102uS. This is the figure I watch to
see if the system is going astray.

The above procedure has basically calibrated the detector.
Now you need to "calibrate" quant. You will need known standards
for this. Most systems do standardless ZAF quant--and do a
pretty good job. For critical work, I use standards and
adjust the SEC factors in Genesis so that ZAF quant works well.
Depending on your specimen, ZAF, PhiZAF or PhiRhoZAF does a
better quant job. Regardless, collect spectra for at least
120 live seconds (the more the better). This will reduce
peak-to-background intensity errors and yield very good quant
results.

Thermo should have a specific set of procedures for your system.
Sounds like the manual is gone.

gary g.



At 10:11 AM 5/12/2005, you wrote:


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From MicroscopyL-request-at-ns.microscopy.com Thu May 12 15:20:52 2005



From: Salamon, Daniel :      Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Thu, 12 May 2005 17:32:30 -0400
Subject: [Microscopy] plastic scintillator recipe

Contents Retrieved from Microscopy Listserver Archives
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Al,

Another good choice would be a zeolite, something like say mordenite or
silicalite (MFI framework) You should be able to get these from a
chemical supplier. They typically have spacings up around 12-18 A. If
you have access to XRD you can double check the lattice parameters.

Incidentally, I think the first lattice image published from TEM was
from a faujasite crystal (also a good choice), a 14.7 A (111) spacing
imaged and reported by Menter (Adv. Phys. 7, 1958 p. 299).

Regards,
Wharton

*************************************************************
Wharton Sinkler, PhD.
UOP LLC
50 E. Algonquin Rd.
Des Plaines IL 60017-5017
mailto: wharton.sinkler-at-uop.com
tel 847-391-3878
fax 847-391-3719

-----Original Message-----
} From: Steve Chapman [mailto:protrain-at-emcourses.com]
Sent: Thursday, May 12, 2005 12:46 PM
To: Al Harmon
Cc: American Soc

Hi

Why not use the graphitised carbon specimen with a 3.44A spacing? A
very
tough specimen but fairly easy to resolve with any modern TEM. Simply
look
for the crystalline appearance in the swirls, shoot for a little
underfocus
(narrow white fringe) and you should be OK. Its probably best to view
at
500,000X plus the binocular to pick out the lattice on the screen and
then
lower the magnification to the level that you require. Provide the
instrument that you are using does not step the diffraction lens between
the
higher and lower levels the original focus will be fine.

I am sure any one of your well known EM accessory suppliers could help
you
out?

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
} From: "Al Harmon" {aharmon-at-mvainc.com}
To: {Microscopy-at-msa.microscopy.com}
Sent: Thursday, May 12, 2005 1:59 PM


Hi Sandeep

You should either read your system manual or contact Thermo, as the procedure differs
from system to system: on some it is done by hardware adjustment, on others all in
software.

cheers

rtch


} From: "Sandeep Kohli" {skohli-at-lamar.colostate.edu}
To: {microscopy-at-microscopy.com}

Hi everyone,

I'm currently working on building a custom ET detector for a FESEM. While trying out different geometries and designs, I though of preparing my own scintillators as I will need several sizes. I will play around with a plastic scintillator (probably p47 powder) and once satisfied with the result, probably switch to YAP.

Does anyone have a good recipe for coating P47 on glass or quartz? What is a reliable supplier for the powder?

Thanks
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-081 ECERF Bldg
9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



From MicroscopyL-request-at-ns.microscopy.com Thu May 12 20:35:20 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 12 May 2005 21:34:05 -0500
Subject: [Microscopy] P47 scintillator powder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Daniel Salamon wrote:
=========================================================
I'm currently working on building a custom ET detector for a FESEM. While
trying out different geometries and designs, I though of preparing my own
scintillators as I will need several sizes. I will play around with a
plastic scintillator (probably p47 powder) and once satisfied with the
result, probably switch to YAP.

Does anyone have a good recipe for coating P47 on glass or quartz? What is a
reliable supplier for the powder?
============================================================
We offer the same P47 powder that is used in the production of the SPI
Supplies Brand of scintillators on URL
http://www.2spi.com/catalog/scintill/p47.shtml
} From that page you will find links to use instructions for the powder and
also the MSDS.

Eventually switching to a YAP (or a YAG) replacement will in fact be a
permanent replacement since the single crystal scintillator should outlast
the lifetime of the FESEM.

Disclaimer: SPI Supplies (in our opinion) has been a "reliable" supplier of
P47 powder for those who coat their own scintillators for many years.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Fri May 13 12:52:17 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 13 May 2005 13:21:41 -0700
Subject: [Microscopy] Re: Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Let us not get too complicated.

Almost every EDS system that I know has a Calibration procedure inbuilt.
You simply need to insert a specimen of any material in the cobalt to copper
density range.

It is important to know where the K alpha and L alpha peaks for the chosen
element are; your books or give away slide rule should provide the answer.

Place the specimen in the microscope and adjust the beam controls to obtain
at least 2,000cps. Go to the Calibration mode, answer the questions and it
should run on its own. If it does not, take down the instructions and come
back to me if you wish?

Good luck

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
} From: "Sandeep Kohli" {skohli-at-lamar.colostate.edu}
To: {microscopy-at-microscopy.com}
Sent: Thursday, May 12, 2005 6:11 PM

Steve,
I have run across some systems that will not work with 2 peaks from the same
element, although that makes the most sense. Going back to when dinosaurs
roamed the earth, we had to use both copper and aluminum to calibrate. If
it is one of those systems that needs 2 elements, just slap a piece of
copper tape on an aluminum stub and scan an area that includes both.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
} From: Steve Chapman [mailto:protrain-at-emcourses.com]
Sent: Friday, May 13, 2005 1:50 PM
To: Sandeep Kohli
Cc: American Soc

Hi

Let us not get too complicated.

Almost every EDS system that I know has a Calibration procedure inbuilt.
You simply need to insert a specimen of any material in the cobalt to copper

density range.

It is important to know where the K alpha and L alpha peaks for the chosen
element are; your books or give away slide rule should provide the answer.

Place the specimen in the microscope and adjust the beam controls to obtain
at least 2,000cps. Go to the Calibration mode, answer the questions and it
should run on its own. If it does not, take down the instructions and come
back to me if you wish?

Good luck

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
} From: "Sandeep Kohli" {skohli-at-lamar.colostate.edu}
To: {microscopy-at-microscopy.com}
Sent: Thursday, May 12, 2005 6:11 PM

It seems to me that one gets out of the EDS system
a direct proportion of the amount of effort put
into it--plus some bonus items based on how
sophisticated it is (newer features or an older generation w/o).
If the specimens are of one general variety, the calibration
procedure can certainly be simple. However, if one is doing
unknowns, and for knowns, wants good results, I think something
more than just two Zs are needed. It would be amusing to
see someone calibrate F at 20KV.

I don't see how calibration can only be two Z peaks.
Each element energy value can be off in the system and
needs to be corrected if so. All of the low Z elements
need to be checked at low KV--or not?

Overall, what am I missing here? If the procedure can
be simplified, how to do that?

gary g.


At 10:50 AM 5/13/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri May 13 17:24:22 2005



From: Rick Mott :      rickmott-at-alumni.princeton.edu
Date: Fri, 13 May 2005 18:23:02 -0400
Subject: [Microscopy] Re: Re: Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary Gaugler wrote:

} I don't see how calibration can only be two Z peaks.
} Each element energy value can be off in the system and
} needs to be corrected if so. All of the low Z elements
} need to be checked at low KV--or not?

As someone who used to do this for a living and has designed
a digital pulse processor, I feel qualified to answer this one.

It is quite possible to calibrate a properly designed modern
digital pulse processor with a single peak. They are exceedingly
linear, and some of them generate an internal "zero peak"
(as do some analog processors) by triggering in the absence
of a photon.

The zero peak eliminates offset errors from the calibration.
The error in gain is a function of the statistics with which
you collect the calibration peak. You can get in trouble by
extrapolating a calibration too far (e.g. using the Cu Ka
and expecting the calibration to be perfect out to 30 KeV
unless you collect for a very long time so the Cu peak can
be located very precisely).

The effect you mention above is not a calibration error
per se. For some detectors, especially older ones, there can
be small peak shifts which are a function of the surface treatment
of the detector crystal, which may affect light element X-rays
which are absorbed close to the surface. It is pretty
consistent for a given detector manufacturer, and most of
them deal with it by simply adjusting the lines table to
compensate for it (carbon, for example, tends to be a
few eV lower than the wavelength tables would lead you
to expect). It isn't really a gain or offset change.

Rick Mott




From MicroscopyL-request-at-ns.microscopy.com Fri May 13 17:37:33 2005



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Fri, 13 May 2005 18:36:18 -0400
Subject: [Microscopy] Re: Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
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EDS energy calibration depends on;

a) the linearity of the pulse processor/preamp electronics.

b) the type of pulse processor (analog or digital) and if it uses a
zero strobe to maintain zero eV calibration.

c) the elements used to preform the energy calibration.


Generally with a analog system you need two peaks, a low energy peak
and a high energy peak. The low energy peak is used for offset
correction, the high energy peak is used for gain correction. This is
known as a two-point calibration

For an EDS system that used a zero strobe (most digital pulse
processors), then only a gain adjustment is required as the offset
(or zero) is maintained by the pulse processor electronics. This is a
one-point calibration.

Performing a manual energy calibration involves adjusting the offset
control (also called zero) such that the low energy peak lines up
with the KLM marker for that peak, then the gain control is adjusted
to lineup the high energy peak with its KLM marker. It's an iterative
process that will converge if the above is follow. There is also a
manual one-point calibration for those systems with zero strobes,
with these only the high peak (gain) is adjusted.

Auto energy calibration routines are simple, pick the elements and
hit the button. Just be sure to look at the results to make sure they
make sense as auto calibration can be fooled by choice of elements
(see below).

If you have an EDS system that requires addition low energy
calibrations (F, O, N, C) after the main energy calibration, then
that indicates that they are doing a non-linear correction of low
energy range. This non-linearity is typically a preamp issue. Some
preamp designs have it, some are perfectly linear.

If you have an EDS system that after a proper one or two point
calibration has element peaks that don't lineup to their KLM markers
(excluding F,O,N,C, see above), then your EDS system has a
non-linearity problem that needs to be fixed.

Additional things that can lead to inaccurate energy calibrations are
the choice of calibration peaks.

For example, Cu has a low energy line (Cu_l) and high energy line
(Cu_k). We are assuming SEMs now, not TEMs. While you might lookup
the Cu_ka1 as 8.046 KeV, the visual Cu_k peak is really at 8.040
because there is an additional CuK line (Cu_ka2) at 8.027 which
convolutes with the first. The resultant visual peak is at 8.040.

Cu_L can fool auto calibration routines if they do not take into
account all the L lines for Cu. There is a visible low level L line
that can downshift the measured peak position. The issue is worse
with a good resolution detector. In fact, just by looking at this
Cu_L (Cu_liiab I think), you can guess the detector resolution, if it
nicely shaped with a peak and valley before rising up the main Cu_L,
then you are at 130eV (MnKa) or better.

We like CuAl at 20-25KeV and 1k to 2k counts per second for energy
calibration, the CuK is good for the high calibration peak, the AlK
is good for the low calibration peak. Both peaks are nicely shaped
and work well for calibration. You will also (sample dependent) get
carbon and oxygen as cross-checks on the low energy peak positions.
We also like having the EDS system tell us the peak position rather
than eyeball using the KLM marker. If I use 8.040 for CuK and the
calibration routines reports 8.040, then we are in sync.


The most important point is EDS energy calibration should be done at
least one a month and the results saved (spectrum included) for
comparison if you have later problems. Some labs do it everyday
before doing any serious analysis. The important point is don't let
energy calibration go for more than a month. If the EDS energy
calibration drifts, then you will have auto peak-id problems as well
as incorrect quant. results because these routines depend on the
energy calibration being correct. Also read your system manuals for
the proper procedure regarding energy calibration, don't guess.

Most service calls we get regarding EDS problems are solved by a
proper energy calibration.

Scott

(who is happy to say he represents 4pi Analysis, Inc.)
--
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From MicroscopyL-request-at-ns.microscopy.com Fri May 13 18:24:15 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 13 May 2005 16:22:48 -0700
Subject: [Microscopy] Re: Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
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the difference of peak values at low Zs is what I
was zeroing in on. Using one high Z and one lower Z
element does not handle all of the Zs. The light
elements are tricky. So I handle them separately.

EDAX uses an EPIC table to adjust the shell eV values
for each element. I don't know what other makers use.
The net effect is to adjust the eV such that detected
and identified peaks work out OK.

I agree that EDAX (no special financial interest) uses
Al and Cu peaks for calibration. Fine. However, does this
fully cover the light elements? I don't think so. So I
do separate analysis at low Z and low KV. If no one cares
about this, don't do it. EDAX DPP-2 is a state-of-the-art
processor. If so, they still have the ability to adjust the
eV values for all elements. So, is this a deficiency adjustment
or a reality adjustment? Whatever. I make the change and
my results are accepted. Otherwise, they are not. Plus,
for the light elements, there is a huge issue with peak pile-up.

I don't see how the system is truly linear. Perhaps I am
just too cautious.

gary g.


At 03:23 PM 5/13/2005, you wrote:


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From MicroscopyL-request-at-ns.microscopy.com Fri May 13 20:08:37 2005



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Fri, 13 May 2005 21:07:20 -0400
Subject: [Microscopy] Re: Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} the difference of peak values at low Zs is what I
} was zeroing in on. Using one high Z and one lower Z
} element does not handle all of the Zs. The light
} elements are tricky. So I handle them separately.
}
} EDAX uses an EPIC table to adjust the shell eV values
} for each element. I don't know what other makers use.
} The net effect is to adjust the eV such that detected
} and identified peaks work out OK.
}
} I agree that EDAX (no special financial interest) uses
} Al and Cu peaks for calibration. Fine. However, does this
} fully cover the light elements? I don't think so. So I
} do separate analysis at low Z and low KV. If no one cares
} about this, don't do it. EDAX DPP-2 is a state-of-the-art
} processor. If so, they still have the ability to adjust the
} eV values for all elements. So, is this a deficiency adjustment
} or a reality adjustment? Whatever. I make the change and
} my results are accepted. Otherwise, they are not. Plus,
} for the light elements, there is a huge issue with peak pile-up.
}
} I don't see how the system is truly linear. Perhaps I am
} just too cautious.
}

One can re-linearize the spectrum by adjusting the eV values in
software. Several companies do this. Some focus on the low end (light
element), some allow all element energies to be "remapped". This is
fine as long as you will always use this exact system for analysis.
So you could say this is fair to get exact calibration. However once
you export the spectrum to some other system or change electronics,
then you will no longer have saved spectra in "calibration". That's
the danger of software linearizing an electronic system by redefining
element peak locations instead of designing a linear electronic
system in the first place.

For example, you on your EDAX with DPP-2 have acquired several
thousand spectra over the year. Opps, lightning strike and the EDAX
is destroyed. You get a new system but it has a different
non-linearity than the previous one. Sure you can set it up to be
correct but what about all those previous spectra (they were backed
up of course). They are not "linear" now and you cannot reanalyze
them with confidence.

Another example, another lab with the same system is down and needs
some analysis performed. You do it and give them the spectra and
results. Their customer wants some things checks and they do it when
they get their system working again. Are your element adjustments
going to match theirs and what's going to happen when they get
different results.

As long as you understand what it means when you adjust the element
position and the ramifications and can accept them. Adjust away.

Rick's comments are correct. crystal effects can down shift low
energy x-rays. However, a correctly designed preamp can correct this.
If your preamp is linear, the rest of the system should be linear.

My viewpoint is light element - ok to allow to software linearize.
Elements above F, it's a system deficiency adjustment and should not
be tolerated. A a state-of-the-art digital pulse processor should
have much better linearity than an analog pulse processor not worse.

Scott
--
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From MicroscopyL-request-at-ns.microscopy.com Fri May 13 22:10:23 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 13 May 2005 20:08:39 -0700
Subject: [Microscopy] Re: Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, I have Genesis Remote and it lives forever. I keep
all .spc spectra files. I see no big deal with long term
validity or manipulation of these data files. They do not
die, nor does my ability to post-evaluate them expire.
This was one of many features of the EDAX system that I
liked (no financial interest here--just a customer).

I use the EPIC adjustment method. It is not necessary
for all elements. But for those that need it, it works.
I can do future evaluations which are basically founded
on raw data collected years before....cool. So, I think
that the historical issue is moot...right?

I don't see that the system is linear. I cannot fathom
why this would be true. OK. Regardless, I compensate for
this at the light elements to be sure that all is OK.
This is probably due to the crystal shifts. Whatever.
The issue if use and results. Personally, I have a method
that works for me and my consumers. If there is a more
streamlined or faster method, please do let me know.
This of course must me ISO-9000 quality.

gary g.




At 06:07 PM 5/13/2005, you wrote:


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From MicroscopyL-request-at-ns.microscopy.com Fri May 13 22:41:45 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 13 May 2005 20:40:12 -0700
Subject: [Microscopy] Re: Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ultimately, it is up to the user to decide
what is sufficient for their needs. It
certainly does not hurt to ask for input
in this regard.

gary g.


At 06:07 PM 5/13/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Sat May 14 10:09:41 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 14 May 2005 08:07:26 -0700
Subject: [Microscopy] Re: Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Steve:

While I am not a Thermo guy (I use EDAX), I've been thinking about
your comments and think that there is a problem here. In this
respect, your suggested approach will produce poor calibration
results.

Some companies use a K and an L for the same element--perhaps Cu for
system calibration? What L alpha is used? Book value? A quick sand pit
I think. L alpha 1 for Cu is .930eV while L beta 1 is .950eV--not all
that far away (two channels at 10eV/channel). The L beta 1 is around
20% of the height of the L alpha 1. Thus, these two peaks combine
to produce a compromised peak with its centroid shifted to the right
by about .936eV. Consequently, one should use K alpha for lower
energy (Al) elements. Furthermore, calibration should not be done
using one element's shell values. Hence, my use of Al and Cu.
This is what EDAX is set up to do--so I did not invent this.
But I see why they did this. There is an unambiguous peak at
8.040KeV (Cu K alpha 1) and another clear peak at 1.486KeV (Al K
alpha 1).

If this is done, then I suppose the system is essentially "calibrated."
However, for critical work, I go further. But that is a personal
decision. Since I get all sorts of films, elements and what not
to examine, two data points won't cut it. But, YMMV.

gary g.




At 10:50 AM 5/13/2005, you wrote:

} Hi
}
} Let us not get too complicated.
}
} Almost every EDS system that I know has a Calibration procedure inbuilt.
} You simply need to insert a specimen of any material in the cobalt to
} copper density range.
}
} It is important to know where the K alpha and L alpha peaks for the chosen
} element are; your books or give away slide rule should provide the answer.
}
} Place the specimen in the microscope and adjust the beam controls to
} obtain at least 2,000cps. Go to the Calibration mode, answer the
} questions and it should run on its own. If it does not, take down the
} instructions and come back to me if you wish?
}
} Good luck
}
} Steve Chapman
} Senior Consultant Protrain
} For electron microscopy consultancy and training world wide
} Tel +44 1280 816512 Fax +44 1280 814007
} Mobile +44 7802 966067 Web www.emcourses.com



From MicroscopyL-request-at-ns.microscopy.com Sat May 14 10:33:16 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 14 May 2005 08:32:13 -0700
Subject: [Microscopy] Non-mechanical thin film polishing

Contents Retrieved from Microscopy Listserver Archives
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Greetings to all:

has anyone encountered a bench top polishing system
for thin metal films? I'm looking for an "affordable"
unit that would hold a standard 12mm diameter pin stub
that has a small chip on it. I would like to polish
this chip for EBSD analysis. I've tried down to
0.02u coloidal silica and alumina and am not getting
the kind of results I seek.

perhaps there is an ion unit like Gatan or JEOL make
that would do this for stub mounts?

User and vendor feedback is welcome.

tnx,
gary g.



From MicroscopyL-request-at-ns.microscopy.com Sun May 15 17:09:19 2005



From: Divna Unipan :      irenarom-at-yahoo.com (by way of MicroscopyListserver)
Date: Sun, 15 May 2005 17:08:06 -0500
Subject: [Microscopy] viaWWW: Life and Dry Blood Analysis course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (irenarom-at-yahoo.com) from http://www.msa.microscopy.com/MLFormMail.html on Sunday, May 15, 2005 at 14:09:10
} -----------------------------------------------------------------------

I would like to take Life and Dry Blood Analysis course from somebody who teach Dr.Enderlein's concept of microscopie. Do you know anybody that teach this course?

Sincerely,
Divna Unipan, ND




From MicroscopyL-request-at-ns.microscopy.com Mon May 16 07:04:39 2005



From: Bruce Luders :      bluders-at-mbl.edu
Date: Mon, 16 May 2005 08:01:45 -0400
Subject: [Microscopy] TEM Immunocytochemistry of Trypanosomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings to All,
We are beginning a TEM study of proteins in T.brucei
and
would appreciate any protocols or references to accomplish
this goal without resorting to cryo.
Thank you
Bruce Luders
Research Assistant
Global Infectious Disease
Marine Biological Labs
7 MBL Street
Woods Hole, MA 02543


From MicroscopyL-request-at-ns.microscopy.com Mon May 16 13:17:38 2005



From: Steve Chapman :      protrain-at-emcourses.com
Date: Mon, 16 May 2005 11:13:59 +0100
Subject: [Microscopy] Re: Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ken

Yes I too go back to the days of aluminium and copper! Perhaps going back
even further I started with a keyboard that simply had EASY keys standing
for Erase, Analyse, Stop, and Yes!

Regards

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
} From: "Ken Converse" {kenconverse-at-qualityimages.biz}
To: "'Steve Chapman'" {protrain-at-emcourses.com} ; "MSA Listserver"
{Microscopy-at-MSA.Microscopy.Com}
Sent: Friday, May 13, 2005 8:39 PM

Hi Gary

I visit a different laboratory, somewhere in the world, on average every
other week, mostly in industry. These people need to be able to look at
"foreign bodies" in their materials and feed their findings back to the
production site. We always calibrate using a two peak method and find that
this is adequate for the tasks in hand.

Modern EDS systems do far more than just move the peaks into line and this
means that our "semi" quant results are also pretty good. My clients vary
from those interested in the defects in wafers, to those looking at aero
engines, aircraft components, road vehicle engines and components, all big
name organisations all working with repetitive materials. We do use
standard materials in each case to check the systems, these standards being
as near as possible to the day to day materials. The instruments in use
often vary in both the SEM manufacturers and the EDS manufacturers. These
clients are all very happy with their results, but perhaps in the more
different world of a university operators would not have the same level of
satisfaction?

Our precautions are that the EDS computing system is always on and that the
liquid nitrogen levels never fall low enough for the system to switch off.
In this way we obtain as near absolute stability as possible, the result is
we rarely see peak drift.

I am interested to see where you find problems?

Regards

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "Steve Chapman" {protrain-at-emcourses.com}
Cc: "MSA listserver" {Microscopy-at-MSA.Microscopy.Com}
Sent: Friday, May 13, 2005 9:21 PM



From MicroscopyL-request-at-ns.microscopy.com Mon May 16 15:42:44 2005



From: Lehman, Ann R :      Ann.Lehman-at-trincoll.edu
Date: Mon, 16 May 2005 16:42:49 -0400
Subject: [Microscopy] anaesthesia agents

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I hope this is a relevant topic for the List. I am seeking a humane,
readily available agent that does not affect ultrastructure for the
purpose of anaesthetization of animals destined for sacrifice following
excision of tissues for SEM and TEM in an undergraduate academic
setting. The animal-use committee (AUC) is getting more stringent about
humane treatment of animal subjects, and they are not happy with my
current methods. What agents are you commonly using to anaesthetize
animals that are ultimately sacrificed??

I have been using a simple inhalant delivery system such as a chloroform
chamber. Following loss of consciousness, and onset of a deep
diaphragmmatic breathing pattern, the animal is fitted with a chloroform
mask and is still breathing and the heart pumping during administration
of fixatives into various organs. It is sacrificed while still
unconscious by means of injection of aldehyde into the heart. This is an
undergraduate setting, and the occasional introduction of fixation
artifacts due to slow fixation or poor penetration actually serves as
examples of the same for classroom discussion, so these crude methods in
fact serve a useful purpose. However, the AUC is not happy with the use
of chloroform or CO2.

The alternatives that have been suggested include ketamine (which I
thought was definitely inhumane, causing paralysis but not loss of
consciousness), halothane (which I'm simply unfamiliar with), or
pentobarbital. I've used the latter and maintained the animal with
ether, but thought the p-b was a restricted substance, and no one here
has the proper license; plus ether is a dangerous substance to store in
the lab.

Any opinions out there? What agents are in common use? How are they
administered? Deep in my dim dusty memory, I recall that some can cause
shifts in ultrastructure, notably in neurological tissues and in
mitochondrial cristae.

Thanks, all, for your opinions and suggestions.

Ann

Ann Lehman
Assistant Director
Electron Microscopy Facility
Mailstop: LSC314
Trinity College
300 Summit Street
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman





From MicroscopyL-request-at-ns.microscopy.com Mon May 16 18:41:07 2005



From: fchu-at-mrl.ubc.ca (by way of MicroscopyListserver)
Date: Mon, 16 May 2005 18:39:54 -0500
Subject: [Microscopy] viaWWW: Epon sections diffused

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (fchu-at-mrl.ubc.ca) from on Monday, May 16, 2005 at 16:25:22
---------------------------------------------------------------------------

Email: fchu-at-mrl.ubc.ca
Name: Fanny Chu

Organization: iCAPTURE Lab, University of British Columbia

Title-Subject: [Microscopy] Epon sections diffused

Question: Somebody in our lab has had sections that will get diffused and dispersed within two seconds staying in the boat. Ironically, all the Epon ingredients were brand new.
Any suggestions what's wrong or can be done about it?


Thanks!

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue May 17 02:09:29 2005



From: Xiaohu Tang :      xiaohutang-at-gmail.com
Date: Tue, 17 May 2005 09:08:13 +0200
Subject: [Microscopy] Video Output of SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Question: Can anyone give me tips about how to get the video output
from SEM (we are using Philips XL30 ESEM). We are using PCs here.

Thanks

With best wishes,

Xiaohu Tang

Microlab
Faculty of Civil Engineering and Geosciences
Delft University of Technology
P.O. Box 5048
2600 GA DELFT, The Netherlands
Email: xiaohutang-at-gmail.com



From MicroscopyL-request-at-ns.microscopy.com Tue May 17 08:46:16 2005



From: Bradley Hacker :      hacker-at-geol.ucsb.edu
Date: Tue, 17 May 2005 06:44:59 -0700
Subject: [Microscopy] UCSB electron microprobe etc. job

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ASSOCIATE DEVELOPMENT ENGINEER

The Department of Geological Sciences at the University of California at Santa
Barbara seeks a motivated and skilled individual to operate, instruct in the
use of, maintain, acquire, and develop electron accelerator and x-ray devices
such as scanning electron microscopes (SEM), electron probe microanalyzers
(EPMA), x-ray spectrometers (EDS), x-ray diffractometers (XRD), electron
diffractometers (EBSD), and optical microscopes. Individuals with post-graduate
education in science or engineering and experience with the chemical and
physical characterization of Earth or engineering materials using the above
methods are particularly encouraged to apply. PhD-level scientists with the
desire to pursue their own research can be accommodated.

This appointment will be as an Associate Development Engineer to begin as early
as September 1, 2005. Review of applications will begin June 1, 2005. Starting
salary range is $48,864–$53,574, depending on education and experience.

Please apply online at http://jobs.ucsb.edu/, and refer to job # 20050186.
Applicants should submit a curriculum vita, a letter of application—including a
detailed description of experience operating and maintaining instrumentation and
objectives in developing and acquiring instrumentation—and provide the names,
email addresses and contact information of three referees. Applicants should
request that the three referees send letters of evaluation directly to:

Bradley Hacker, Chair
Search Committee
Department of Geological Sciences
University of California
Santa Barbara, CA 93106-9630 

Questions can be directed to Professor Bradley Hacker, hacker-at-geol.ucsb.edu

UCSB is an Equal Opportunity/Affirmative Employer

-----------------------------------------------------------
Bradley R. Hacker office: Webb 2120 tel 805.893.7952
Professor of Geology dept 805.893.3471 fax 805.893.2314
Geological Sciences & Institute for Crustal Studies
University of California, Santa Barbara CA 93106-9630
http://www.geol.ucsb.edu/faculty/hacker/




From MicroscopyL-request-at-ns.microscopy.com Tue May 17 08:49:03 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 17 May 2005 08:47:31 -0500
Subject: [Microscopy] viaWWW: Epon sections diffused

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This sounds like a classic case of poor infiltration. I would suggest
using more changes of pure resin before curing the blocks and leaving
the specimens in the resin longer during infiltration steps. Use of a
microwave to facilitate infiltration can also be a great help.

What kind of specimen are you having trouble with? Some tissues are
notoriously difficult to infiltrate.

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
} From: by way of MicroscopyListserver [mailto:fchu-at-mrl.ubc.ca]
Sent: Monday, May 16, 2005 6:40 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (fchu-at-mrl.ubc.ca) from on Monday, May 16, 2005 at
16:25:22
------------------------------------------------------------------------
---

Email: fchu-at-mrl.ubc.ca
Name: Fanny Chu

Organization: iCAPTURE Lab, University of British Columbia

Title-Subject: [Microscopy] Epon sections diffused

Question: Somebody in our lab has had sections that will get diffused
and dispersed within two seconds staying in the boat. Ironically, all
the Epon ingredients were brand new.
Any suggestions what's wrong or can be done about it?


Thanks!

------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Tue May 17 09:04:33 2005



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Tue, 17 May 2005 10:03:19 -0400
Subject: [Microscopy] Re: Video Output of SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
} Question: Can anyone give me tips about how to get the video output
} from SEM (we are using Philips XL30 ESEM). We are using PCs here.
}

The XL30 has an BNC that outputs standard NTSC (RS-170) video format
on the back of the scope, I forget the connector name, it's near the
top of one of the rack cards. Just run that into any USB or PCI video
capture hardware (or video monitor). It might be PAL video format
instead of NTSC video format for non-USA customers, the XL30 hardware
can output both but I'm only familiar with the USA variant. It's
normal use is to drive a small electrostatic video printer for quick
low resolution hardcopy.

Be aware that the resolution will be limited by the NTSC/PAL video
format. 640 x 480 interlaced for NTSC. If you want a full resolution
digital image capture either use the native XL30 capture (remember to
XLStretch the image or it will have non-square pixels when you
display it on Win32 PC) or get an external digital image capture
system. In the case of the XL30, you need an active scan system or
you will suffer the same non-square pixel issue on capture.

Scott
--
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From MicroscopyL-request-at-ns.microscopy.com Tue May 17 09:53:06 2005



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 17 May 2005 09:51:49 -0500
Subject: [Microscopy] Re: EDS Calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You had a keyboard! I think we only had buttons and switches.

I can't even remember all the details of the first EDS system I worked
with. I can't even remember if it was EDAX or Nuclear Diodes at the time. I
recall that it had a 2-inch, green, monochrome CRT with alternating
patterns of 10 bright dots and 10 not-so-bright dots. I think the standard
resolution was 100 eV/channel and we had to count the numbers of groups of
dots to determine the energy of the peak, for example iron peaked in the
4th channel of the 7th group (i.e., channel 64). Peak ID got tough when the
pots got noisy and the spectrum would jump. We would have to start counting
over. There were no energy limits or cursor for the screen, let alone MLK
markers. There was no computer. The only storage was an optional 2-inch
paper tape printout with one intensity record per line.

Oh, the good old days . . . Not! It is sometimes helpful to remember just
how far we have come. It can help us to appreciate what we have.

Warren

At 05:16 AM 05/16/05, Steve Chapman wrote:

} Hi Ken
}
} Yes I too go back to the days of aluminium and copper! Perhaps going back
} even further I started with a keyboard that simply had EASY keys standing
} for Erase, Analyse, Stop, and Yes!
}
} Regards
}
} Steve Chapman
} Senior Consultant Protrain
} For electron microscopy consultancy and training world wide
} Tel +44 1280 816512 Fax +44 1280 814007
} Mobile +44 7802 966067 Web www.emcourses.com
}
} ----- Original Message -----
} } From: "Ken Converse" {kenconverse-at-qualityimages.biz}
} To: "'Steve Chapman'" {protrain-at-emcourses.com} ; "MSA Listserver"
} {Microscopy-at-MSA.Microscopy.Com}
} Sent: Friday, May 13, 2005 8:39 PM
} Subject: [Microscopy] RE: Re: EDS Calibration
}
}
} } Steve,
} } I have run across some systems that will not work with 2 peaks from the same
} } element, although that makes the most sense. Going back to when dinosaurs
} } roamed the earth, we had to use both copper and aluminum to calibrate. If
} } it is one of those systems that needs 2 elements, just slap a piece of
} } copper tape on an aluminum stub and scan an area that includes both.
} }
} } Ken Converse
} }
} } owner
} } QUALITY IMAGES
}
} -------------------------------------------
} No files should be attached to this message
} -------------------------------------------
} Warren E. Straszheim, Ph.D.
} Materials Analysis and Research Lab
} Iowa State University
} 46 Town Engineering
} Ames IA, 50011-3232
}
} Ph: 515-294-8187
} FAX: 515-294-4563
}
} E-Mail: wesaia-at-iastate.edu
} Web: www.marl.iastate.edu
}
} Scanning electron microscopy, x-ray analysis, and image analysis of materials
} Computer applications and networking



From MicroscopyL-request-at-ns.microscopy.com Tue May 17 10:10:47 2005



From: Norman Charnley :      norman-at-earth.ox.ac.uk
Date: Tue, 17 May 2005 16:09:25 +0100
Subject: [Microscopy] ISI-40 SEM and accessories available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We intend to dispose of our old (mid 1980s) ISI-40 SEM, equipped with
Robinson backscatter detector, Deben Pixie-8 frame store with Sony video
printer, and Link 860 series 2 Si(Li) EDS (with 8 inch floppy disks!)

Although it has not been used for some time, it was a working instrument
in routine use. I would be surprised if anyone wanted the whole thing,
but useful for spares if you're trying to keep one going - please
contact me if interested.

--
Dr. Norman Charnley
Department of Earth Sciences
University of Oxford
Oxford OX1 3PR, UK.

+44 1865 272053 (Office)
+44 1865 272009/283741/272041 (SEM/Microprobe/Stable Isotope Labs)

Fax: +44 1865 272072


From MicroscopyL-request-at-ns.microscopy.com Tue May 17 10:19:22 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 17 May 2005 11:16:18 -0400
Subject: [Microscopy] Re: viaWWW: Epon sections diffused

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sounds like poor infiltration to me. To be more specific I think most
people on the List would like to know:
What kind of tissue?
How was it fixed?
How large are the pieces of tissue?
How was it processed?

Geoff

by way of MicroscopyListserver wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From MicroscopyL-request-at-ns.microscopy.com Tue May 17 16:09:31 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 17 May 2005 17:06:29 -0400
Subject: [Microscopy] Re: AskAMicroscopist: help a college student for his

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't think this question can be answered in this forum, it is
just too big. If there is no one at your institution who can provide the
necessary guidance, another project may be in order.
If you have an ESEM I would expect that someone there knows how to
prepare specimens for it??

Geoff

by way of Ask-A-Microscopist wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From MicroscopyL-request-at-ns.microscopy.com Wed May 18 07:24:42 2005



From: ilanh-at-patholog.tau.ac.il (by way of MicroscopyListserver)
Date: Wed, 18 May 2005 07:23:28 -0500
Subject: [Microscopy] viaWWW: Kodak film problem SO163

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ilanh-at-patholog.tau.ac.il) from http://www.microscopy.com/MLFormMail.html on Wednesday, May 18, 2005 at 01:33:26
---------------------------------------------------------------------------

Email: ilanh-at-patholog.tau.ac.il
Name: Ilan Hammel

Organization: Tel Aviv University

Title-Subject: [Microscopy] [Filtered] Kodak film problem SO163

Question: I have some Kodak SO-163 film, 6.5 x 9 cm, Lot 2005-01 17363870 (I think) and I found that the notch was in the wrong place so that the film got put into the casettes with the emusion side down (instead of up) and I thought something was wrong with the camera. I ended up spending a lot of money trying to fix a camera problem I did not have.

I was just wondering if this was a problem others experienced or am I the only one who encountered this problem?

I am trying to work with our vendor to get replaced the wasted film exposures and also, compensation for the wasted money with the TEM service engineers to find a problem with the camera that we did not really have.

ILAN HAMMEL D.Sc.
Department of Pathology
Sackler Faculty of Medicine
Sackler Building, room: 425
Tel Aviv University
P.O. Box 39040, Ramat Aviv
Tel-Aviv 69978, Israel.

Tel: (972)-3-6408408
Tel: (972)-3-6409861
Fax: (972)-3-6409141
Email: ilanh-at-patholog.tau.ac.il

http://www.tau.ac.il/medicine/pathology/hammel.html



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed May 18 07:25:20 2005



From: emily.wiesner-at-medecine.unige.ch (by way of MicroscopyListserver)
Date: Wed, 18 May 2005 07:23:57 -0500
Subject: [Microscopy] viaWWW: Cryostat sectioning of rat brains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (emily.wiesner-at-medecine.unige.ch) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, May 18, 2005 at 05:38:05
---------------------------------------------------------------------------

Email: emily.wiesner-at-medecine.unige.ch
Name: Emily Camm

Organization: Uni. of Geneva

Title-Subject: [Microscopy] [Filtered] MListserver: Cryostat sectioning of rat brains

Question: Hello,
I was wondering whether anyone could advise me of the best way to collect rat brain sections when cutting on a cryostat. I am placing the slide down to collect the section, but the section either folds, wrinkles or get small bubbles underneath it. The brains have been perfusion fixed with PFA and frozen in OCT. I have been cutting at -20 degrees.
Help!
Emily

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed May 18 09:26:22 2005



From: David Patton :      David.Patton-at-uwe.ac.uk
Date: Wed, 18 May 2005 15:24:33 +0100
Subject: [Microscopy] AskAMicroscopist: help a college student for his project

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Try this method based on urinary catheters.

Rinse in distilled water

Blot underside of sample and mount on ESEM stub with a carbon tab
Put distilled water on sample so it does not dehydrate during pump down

Adjust WD to 10mm

Set Peltier stage to 5 degrees C

Pump down to 7.5 torr and flood then reduce pressure to 7.4 torr and so on to 6.5 torr.

Turn beam on and find top of sample (the hard bit! especially if water has condensed on sample!). With help of the ESEM operator set WD to 7.5mm.

Now slowly reduce pressure to expose sample. If you go too fast sample will be exposed and dehydrate instantly. Try to hold sample at a pressure where no further dehydration takes place (tricky). The correct pressure for the stub alone is 6.5 torr. Biological samples would probably equilibrate after a few hours. I am too impatient and go below 6.5 torr and reverse the pressure if change is too rapid.

Good luck

Dave


-----Original Message-----
} From: by way of Ask-A-Microscopist [mailto:john-rong-at-ouhsc.edu]
Sent: 09 May 2005 23:44
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (john-rong-at-ouhsc.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, May 9, 2005 at 14:12:12
---------------------------------------------------------------------------

Email: john-rong-at-ouhsc.edu
Name: John Rong

Organization: University of Oklahoma Health Sciences Center

Education: Graduate College

Location: Oklahoma City, Oklahoma

Question: I was asked to help a college student for his project. I am a professor but unfortunately I have no knowledge about this topic. I would appreciate any help you can provide so that I can pass the information to the student. Thanks!

The student plans to observe the surface structures of a vascular stent that is implanted inside a pigís coronary artery. The sample has been stored in ìformalinî (10% of formaldehyde) for more than 6 months. The microscope is an Environmental Scanning Electron Microscope.

This student would like to learn the protocols and procedures for preparing his sample. For properly operating the microscope, what special procedures or steps have to be followed and necessary operating conditions such as temperature, ATM, humidity, etc. to be maintained?

Thanks again.


---------------------------------------------------------------------------




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From MicroscopyL-request-at-ns.microscopy.com Wed May 18 10:14:33 2005



From: Karl Hagglund :      hagglundk1-at-nku.edu
Date: Wed, 18 May 2005 11:13:20 -0400
Subject: [Microscopy] SEM Old Anti vibration equip

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are in the process of clearing out some space in an old building and
found a Kinetic Systems anti vibration platform that was originally used
with a Hitachi 570 SEM. It has three legs/pistons driven either by
pressurized N2 or a compressor and a 26" x 32" steel plate on which the
microscope column unit sat. I am unsure of the age of the system, but
the microscope was originally purchased around 1986.

Our new lab is adequately isolated in terms of vibration, so we do not
see an immediate need for the unit. The university has a surplus
equipment area, but it seems unlikely it will ever find an interested
party using its traditional channels. I thought I would pose a question
to the list to find out what others have done in these situations.

1. Is there a market for used systems like this, and can someone
recommend an approximate value for a used anti vibration platform?

2. Have folks gone to the trouble to post the information to the surplus
equipment forum and get rid of it themselves, or have the people in
charge of university surplus taken on this role?

Thanks for your help in this matter.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu



From MicroscopyL-request-at-ns.microscopy.com Wed May 18 10:20:26 2005



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 18 May 2005 17:15:15 +0200
Subject: [Microscopy] Feedback on Nikon DS-L1 unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

Having some money to buy a camera for our OM, I would be interested on
some feedback about the little Nikon DS-L1 stand-alone camera controle
unit, which has network connection, and alowed to work without a PC near
the OM. As we need something which can be easy moved between a inverted
OM, an upright one and a binocular, it seems interesting to have such
little "toy". But what about it in a real use ? Our use is mainly for
control in SEM and TEM sample prepartion, little real OM works.


All advices are welcome.

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From MicroscopyL-request-at-ns.microscopy.com Wed May 18 11:05:43 2005



From: Christopher Gilpin :      christopher.gilpin-at-utsouthwestern.edu
Date: Wed, 18 May 2005 11:02:23 -0500
Subject: [Microscopy] Open Technician position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers
UT Southwestern Medical Center at Dallas has an open position for an
electron microscopy technician.
Details and online applications can be found on our HR website
http://www8.utsouthwestern.edu/utsw/cda/dept36801/files/151313.html

Also see the Imaging facility web pages for more information about the lab

http://www4.utsouthwestern.edu/mcif/index.htm

Regards

Chris

Christopher J Gilpin Ph.D.
Assistant Professor
Director, Molecular and Cellular Imaging Facility
K1.A04
UT Southwestern Medical Center at Dallas
5323 Harry Hines Boulevard
Dallas, TX 75390-9039
Phone +1 214 648 2827
Fax +1 214 648 6408



From MicroscopyL-request-at-ns.microscopy.com Wed May 18 11:24:49 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 18 May 2005 13:45:09 -0700
Subject: [Microscopy] Condenser aperture contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ilan,
A similar thing happened to me with the Kodak 4489 films several years ago.
Several boxes of TEM film had the notch on the wrong side, but I noticed
because the film emulsion is a different gray under the darkroom safe light,
so I just turned the film over so the emulsion was up. I sent them a reply
card noting the problem, but never heard anything back. The notch has been
in the right place ever since.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
} From: "by way of MicroscopyListserver" {ilanh-at-patholog.tau.ac.il}
To: {microscopy-at-microscopy.com}
Sent: Wednesday, May 18, 2005 5:23 AM

Dear Lists,
We have been having problems with contamination of the 150 um aperture
on our FEI F30H Polara microscope. No other apertures, condenser or
otherwise, have become contaminated, but the 150 has had problems
several times. (It has been the aperture most frequently used, which
could be why.) Furthermore, our instrument is used almost exclusively
with frozen-hydrated biological specimens, and the only other specimen
in use recently was 10 nm colloidal gold on a R 2/2 Quantifoil. Has
anyone else experienced problems with condenser aperture contamination
on a Polara?
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed May 18 17:48:45 2005



From: winston.wiggins-at-cshs.org (by way of MicroscopyListserver)
Date: Wed, 18 May 2005 17:47:16 -0500
Subject: [Microscopy] viaWWW: Kodak film problem SO163

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (winston.wiggins-at-cshs.org) from http://www.microscopy.com/MLFormMail.html on Wednesday, May 18, 2005 at 10:35:45
---------------------------------------------------------------------------

Email: winston.wiggins-at-cshs.org
Name: Winston Wiggins

Organization: Cedars-Sinai Medical Center; Los Angeles, CA

Title-Subject: [Microscopy] [Filtered] MListserver: Kodak film problem SO163

Question: Ilan,
We had the same problem here with our Kodak SO-163. Fortunately we found out really quickly. When the negs came out ìunexposedî except for the sequence numbers, one of us decided to check to see where the emulsion was. It apparently wasnít where it was supposed to be, according to the ìnotchî position. It seems to have been a one-time problem so we didnít record film batch numbers, etc. We did call Kodak though!
Winston


---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Wed May 18 17:48:55 2005



From: mccaulak-at-wfu.edu (by way of MicroscopyListserver)
Date: Wed, 18 May 2005 17:47:45 -0500
Subject: [Microscopy] viaWWW: searching for window insert to block light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mccaulak-at-wfu.edu) from http://www.msa.microscopy.com/MLFormMail.html on Wednesday, May 18, 2005 at 11:48:58
---------------------------------------------------------------------------

Email: mccaulak-at-wfu.edu
Name: Anita McCauley

Organization: Wake Forest University

Title-Subject: [Microscopy] [Filtered] MListserver: searching for window insert to block light

Question: I am setting up a room for fluorescence microscopy which has a window to the outside. I need to find a way to block the light coming through this window. There is an insert in another room in the facility which functions very well to block the light entering that room but nobody remembers when it was purchased or the vendor. Does anybody know where I can get an insert to block the light or have suggestions for other ways to accomplish this?

Thanks.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed May 18 19:42:42 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 18 May 2005 17:40:20 -0700
Subject: [Microscopy] viaWWW: Kodak film problem SO163

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Every container of film (of any type) I have ever seen has a disclaimer
that the vendor liability is limited to replacing the film, no matter
what trouble it causes. It stinks, but this is a good illustration of
defective film causing a lot of trouble. My sympathies.

--John Mardinly

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:ilanh-at-patholog.tau.ac.il]
Sent: Wednesday, May 18, 2005 5:23 AM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ilanh-at-patholog.tau.ac.il) from
http://www.microscopy.com/MLFormMail.html on Wednesday, May 18, 2005 at
01:33:26
------------------------------------------------------------------------
---

Email: ilanh-at-patholog.tau.ac.il
Name: Ilan Hammel

Organization: Tel Aviv University

Title-Subject: [Microscopy] [Filtered] Kodak film problem SO163

Question: I have some Kodak SO-163 film, 6.5 x 9 cm, Lot 2005-01
17363870 (I think) and I found that the notch was in the wrong place so
that the film got put into the casettes with the emusion side down
(instead of up) and I thought something was wrong with the camera. I
ended up spending a lot of money trying to fix a camera problem I did
not have.

I was just wondering if this was a problem others experienced or am I
the only one who encountered this problem?

I am trying to work with our vendor to get replaced the wasted film
exposures and also, compensation for the wasted money with the TEM
service engineers to find a problem with the camera that we did not
really have.

ILAN HAMMEL D.Sc.
Department of Pathology
Sackler Faculty of Medicine
Sackler Building, room: 425
Tel Aviv University
P.O. Box 39040, Ramat Aviv
Tel-Aviv 69978, Israel.

Tel: (972)-3-6408408
Tel: (972)-3-6409861
Fax: (972)-3-6409141
Email: ilanh-at-patholog.tau.ac.il

http://www.tau.ac.il/medicine/pathology/hammel.html



------------------------------------------------------------------------
---




From MicroscopyL-request-at-ns.microscopy.com Wed May 18 20:42:50 2005



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 18 May 2005 21:38:32 -0400
Subject: [Microscopy] Re: viaWWW: searching for window insert to block light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Black Spray paint? I've also seen Al foil taped to the window. ...or do
you want a removable light barrier?

Henk

At 06:47 PM 5/18/2005, mccaulak-at-wfu.edu wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hendrik O. Colijn colijn.1-at-osu.edu
OSU Campus Electron Optics Facility www.ceof.ohio-state.edu
040 Fontana Labs, 116 W. 19th Ave



From MicroscopyL-request-at-ns.microscopy.com Thu May 19 02:24:25 2005



From: Aarti Harle :      aarti_harle-at-yahoo.co.in
Date: Thu, 19 May 2005 00:23:10 -0700 (PDT)
Subject: [Microscopy] TEM/ SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everybody

We are planning to buy new
1) Transmission Electron Microscope, 200 Kv with EELS
2)and Scanning Electron Microscope
as our old electron m/scope are now antrique peice.

Please share your knowledge and experience with
different models and make particularly with
Philips/FEI and Jeol or any other make with excellent
features

Regards
Arti Harle
Institute of Microbial Technology
Chandigarh
India


Ms.ARTI HARLE/ SCIENTIFIC ASSISTANT
REGIONAL SOPHISTICATED INSTURMENTATION CENTER
INDIAN INSTITUTE OF TECHNOLOGY-BOMBAY
POWAI, MUMBAI - 400076. INDIA
E-MAIL : aarti_harle-at-yahoo.co.in, aartih-at-pawan.cc.iitb.ac.in
PHONE : 91-22-5767691,
RESI.: 91-22-5720109



Yahoo! Mail
Stay connected, organized, and protected. Take the tour:
http://tour.mail.yahoo.com/mailtour.html



From MicroscopyL-request-at-ns.microscopy.com Thu May 19 05:18:23 2005



From: David Patton :      David.Patton-at-uwe.ac.uk
Date: Thu, 19 May 2005 11:16:47 +0100
Subject: [Microscopy] TEM/ SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It depends on the nature of your samples. I would consider an ESEM or
equivalent. If you can afford field emission even better.

Dave

-----Original Message-----
} From: Aarti Harle [mailto:aarti_harle-at-yahoo.co.in]
Sent: 19 May 2005 08:23
To: microscopy-at-msa.microscopy.com

Hello Everybody

We are planning to buy new
1) Transmission Electron Microscope, 200 Kv with EELS
2)and Scanning Electron Microscope
as our old electron m/scope are now antrique peice.

Please share your knowledge and experience with
different models and make particularly with
Philips/FEI and Jeol or any other make with excellent
features

Regards
Arti Harle
Institute of Microbial Technology
Chandigarh
India


Ms.ARTI HARLE/ SCIENTIFIC ASSISTANT
REGIONAL SOPHISTICATED INSTURMENTATION CENTER
INDIAN INSTITUTE OF TECHNOLOGY-BOMBAY
POWAI, MUMBAI - 400076. INDIA
E-MAIL : aarti_harle-at-yahoo.co.in, aartih-at-pawan.cc.iitb.ac.in
PHONE : 91-22-5767691,
RESI.: 91-22-5720109



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From MicroscopyL-request-at-ns.microscopy.com Thu May 19 06:18:27 2005



From: frank.karl-at-degussa.com
Date: Thu, 19 May 2005 07:16:14 -0400
Subject: [Microscopy] Re: viaWWW: searching for window insert to block light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Back in the days (20 years ago) when I used a darkroom for TEM negatives,
we used a special window shade. It was designed for day time sleepers and
consisted of three layers. Two white outer layers and a thicker black
inner layer. Light simply did not penetrate it. It was designed for
people who work nights and sleep during the day. We had a "track" build to
hold the edges down and it worked fine for years.

Good luck!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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attachments without reading or saving in any manner. Thank you.



mccaulak-at-wfu.edu
(by way of To: microscopy-at-microscopy.com
MicroscopyListser cc:
ver) Subject: [Microscopy] viaWWW: searching for window insert to block light

05/18/2005 06:47
PM







------------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mccaulak-at-wfu.edu) from
http://www.msa.microscopy.com/MLFormMail.html on Wednesday, May 18, 2005 at
11:48:58
---------------------------------------------------------------------------

Email: mccaulak-at-wfu.edu
Name: Anita McCauley

Organization: Wake Forest University

Title-Subject: [Microscopy] [Filtered] MListserver: searching for window
insert to block light

Question: I am setting up a room for fluorescence microscopy which has a
window to the outside. I need to find a way to block the light coming
through this window. There is an insert in another room in the facility
which functions very well to block the light entering that room but nobody
remembers when it was purchased or the vendor. Does anybody know where I
can get an insert to block the light or have suggestions for other ways to
accomplish this?

Thanks.

---------------------------------------------------------------------------






From MicroscopyL-request-at-ns.microscopy.com Thu May 19 08:30:23 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Thu, 19 May 2005 08:29:05 -0500
Subject: [Microscopy] Re: viaWWW: searching for window insert to block light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anita,

I just put aluminum foil over the window. Doesn't allow for easy
removal to let in light, but the darkness is complete. Worked so well
during the 24 hour-daylight-summer in the Arctic that it was hard to
get out of bed in the "morning".

Phil

} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Microscopy] [Filtered] MListserver: searching for
} window insert to block light
}
} Question: I am setting up a room for fluorescence microscopy which
} has a window to the outside. I need to find a way to block the
} light coming through this window. There is an insert in another
} room in the facility which functions very well to block the light
} entering that room but nobody remembers when it was purchased or the
} vendor. Does anybody know where I can get an insert to block the
} light or have suggestions for other ways to accomplish this?
}
} Thanks.
}
} ---------------------------------------------------------------------------

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm


From MicroscopyL-request-at-ns.microscopy.com Thu May 19 09:37:00 2005



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Thu, 19 May 2005 09:35:23 -0500 (CDT)
Subject: [Microscopy] Re: viaWWW: searching for window insert to block light

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When we needed to block out windows in our facility, we went to our local
building supply store and bought a 4 x 8 ft. sheet of 1 inch thick
insulation board that had foil facing on one side and black plastic facing
on the other (we used Dow Supertuff R, but there are no doubt others that
would work). Then we went to the fabric store for a roll of thin quilt
batting and some black pseudo-suede polyester fabric. We cut the
insulation board to fit in the window frame, covered the cut piece with
batting (front only, not around the sides), then put fabric over the
batting around the sides to the back and used a staple gun to secure the
fabric to the back. We then pushed it into the window frame. They
fit tightly because of the fabric, look pretty good, and were quite
inexpensive. Since our windows are not visible to the outside because of
our location, we didn't bother making them look good from the outside, but
you could always paint the windows before installing the foam boards if
this is an issue for you.

Heather Owen



On Wed, 18 May 2005, by way of MicroscopyListserver wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mccaulak-at-wfu.edu) from http://www.msa.microscopy.com/MLFormMail.html on Wednesday, May 18, 2005 at 11:48:58
} ---------------------------------------------------------------------------
}
} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Microscopy] [Filtered] MListserver: searching for window insert to block light
}
} Question: I am setting up a room for fluorescence microscopy which has a window to the outside. I need to find a way to block the light coming through this window. There is an insert in another room in the facility which functions very well to block the light entering that room but nobody remembers when it was purchased or the vendor. Does anybody know where I can get an insert to block the light or have suggestions for other ways to accomplish this?
}
} Thanks.
}
} ---------------------------------------------------------------------------
}

Dr. Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
Lapham Hall, P.O. Box 413
Milwaukee, WI 53210
USA

Phone: (414)229-6816



From MicroscopyL-request-at-ns.microscopy.com Thu May 19 12:05:12 2005



From: Kenneth Livi :      klivi-at-jhu.edu
Date: Thu, 19 May 2005 13:03:35 -0400
Subject: [Microscopy] TEM: rotation holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are interested in obtaining a used FEI compustage or
non-compustage tilt rotation holder. We have some holders that may be
considered in a trade, or reasonable offers would be entertained.
Thanks.
Ken


From MicroscopyL-request-at-ns.microscopy.com Thu May 19 17:36:41 2005



From: Rosemary White :      Rosemary.White-at-csiro.au
Date: Fri, 20 May 2005 08:36:19 +1000
Subject: [Microscopy] Re: Re: viaWWW: searching for window insert to

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You may well find, as we did a couple of years ago, that the local window
blind companies have the 100% blockout blind material and all the other
materials to make the blinds that track down the edge of the window frame.
All they need to do is measure your window to make one for you. We got one
made for an office that's used occasionally for fluorescence microscopy.
Got a small blind made for the window in the office door, too....
cheers,
Rosemary

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia


} From: Heather A Owen {owenha-at-csd.uwm.edu}
} Date: Thu, 19 May 2005 09:35:23 -0500 (CDT)
} To: by way of MicroscopyListserver {mccaulak-at-wfu.edu}
} Cc: microscopy-at-microscopy.com
} Subject: [Microscopy] Re: viaWWW: searching for window insert to block light
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} When we needed to block out windows in our facility, we went to our local
} building supply store and bought a 4 x 8 ft. sheet of 1 inch thick
} insulation board that had foil facing on one side and black plastic facing
} on the other (we used Dow Supertuff R, but there are no doubt others that
} would work). Then we went to the fabric store for a roll of thin quilt
} batting and some black pseudo-suede polyester fabric. We cut the
} insulation board to fit in the window frame, covered the cut piece with
} batting (front only, not around the sides), then put fabric over the
} batting around the sides to the back and used a staple gun to secure the
} fabric to the back. We then pushed it into the window frame. They
} fit tightly because of the fabric, look pretty good, and were quite
} inexpensive. Since our windows are not visible to the outside because of
} our location, we didn't bother making them look good from the outside, but
} you could always paint the windows before installing the foam boards if
} this is an issue for you.
}
} Heather Owen
}
}
}
} On Wed, 18 May 2005, by way of MicroscopyListserver wrote:
}
} }
} }
} }
-----------------------------------------------------------------------------} }
-
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------------
} } --
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was submitted
} } by (mccaulak-at-wfu.edu) from http://www.msa.microscopy.com/MLFormMail.html on
} } Wednesday, May 18, 2005 at 11:48:58
} } ---------------------------------------------------------------------------
} }
} } Email: mccaulak-at-wfu.edu
} } Name: Anita McCauley
} }
} } Organization: Wake Forest University
} }
} } Title-Subject: [Microscopy] [Filtered] MListserver: searching for window
} } insert to block light
} }
} } Question: I am setting up a room for fluorescence microscopy which has a
} } window to the outside. I need to find a way to block the light coming
} } through this window. There is an insert in another room in the facility
} } which functions very well to block the light entering that room but nobody
} } remembers when it was purchased or the vendor. Does anybody know where I can
} } get an insert to block the light or have suggestions for other ways to
} } accomplish this?
} }
} } Thanks.
} }
} } ---------------------------------------------------------------------------
} }
}
} Dr. Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} Lapham Hall, P.O. Box 413
} Milwaukee, WI 53210
} USA
}
} Phone: (414)229-6816
}
}


From MicroscopyL-request-at-ns.microscopy.com Thu May 19 17:57:00 2005



From: v_bleu_knight-at-yahoo.com (by way of MicroscopyListserver)
Date: Thu, 19 May 2005 17:55:47 -0500
Subject: [Microscopy] viaWWW: Using permanox chamber slides for embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (v_bleu_knight-at-yahoo.com) from http://microscopy.com/MLFormMail.html on Thursday, May 19, 2005 at 11:00:27
---------------------------------------------------------------------------

Email: v_bleu_knight-at-yahoo.com
Name: Bleu Knight

Organization: New Mexico State University

Title-Subject: [Microscopy] [Filtered] MListserver:TEM- Using permanox chamber slides for embedding

Question: Has anyone previously used permanox chambered slides to embed cultured cells for TEM? Any advice on this matter will be greatly appreciated (Especially creating blocks of appropriate size). Thanks!

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu May 19 17:58:44 2005



From: v_bleu_knight-at-yahoo.com (by way of MicroscopyListserver)
Date: Thu, 19 May 2005 17:57:30 -0500
Subject: [Microscopy] viaWWW: labeling biological samples with Quantum Dots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (v_bleu_knight) from http://microscopy.com/MLFormMail.html on Thursday, May 19, 2005 at 11:04:16
---------------------------------------------------------------------------

Email: v_bleu_knight
Name: Bleu Knight

Organization: New Mexico State University

Title-Subject: [Microscopy] [Filtered] MListserver:TEM- labeling biological samples with Quantum Dots

Question: Hello, I am going to label cultured cells with quantum dots and look at them with the TEM. Does anyone know if it is possible to impart electron density to the biological tissue and still visualize the Quantum dots? I am planning to use Osmium, Lead Citrate, and Uranyl acetate but I do not know if these compounds will react with the quantum dots or obstruct their visualization. I would appreciate any advice on this issue

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu May 19 17:59:22 2005



From: beagleyg-at-alma.edu (by way of MicroscopyListserver)
Date: Thu, 19 May 2005 17:57:57 -0500
Subject: [Microscopy] viaWWW: stuck film chamber door

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (beagleyg-at-alma.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, May 19, 2005 at 15:38:09
---------------------------------------------------------------------------

Email: beagleyg-at-alma.edu
Name: gwyneth beagley

Organization: department of psychology alma college

Title-Subject: [Microscopy] [Filtered] stuck film chamber door

Question: Has anyone else experienced a "stuck" door on a film chamber of a JEOL 1200 EX? Should I force it? The latch turns; is the failure to open due to too much vacuum or air not being pumped into the chamber? Everything else seems to be working, so I am rather confused. Thanks. g

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu May 19 20:04:23 2005



From: Scott Walck :      swalck-at-comcast.net
Date: Thu, 19 May 2005 20:59:34 -0400
Subject: [Microscopy] viaWWW: stuck film chamber door

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If I remember correctly, the door latch on the 1200EX (the same as the
2000FX) is a mechanical latch with a microswitch on it to activate the
vacuum logic circuitry. When the chamber is up to air, the door just pops
open. Monitor your vacuum page on the monitor. (I think that it is page
3.) If the valves don't cycle for the column and then the up-to-air valve
opens, then you have a problem. You should also hear the valve between the
column and the chamber close (it's the big "clunk" noise.) If the chamber
is under vacuum and the valves are not in the correct positions, forcing the
door open will not be a good thing for your diffusion pump. Call service.
They've seen it before and they might have a quick fix for you.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127

-----Original Message-----
} From: by way of MicroscopyListserver [mailto:beagleyg-at-alma.edu]
Sent: Thursday, May 19, 2005 6:58 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (beagleyg-at-alma.edu) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday,
May 19, 2005 at 15:38:09
---------------------------------------------------------------------------

Email: beagleyg-at-alma.edu
Name: gwyneth beagley

Organization: department of psychology alma college

Title-Subject: [Microscopy] [Filtered] stuck film chamber door

Question: Has anyone else experienced a "stuck" door on a film chamber of a
JEOL 1200 EX? Should I force it? The latch turns; is the failure to open due
to too much vacuum or air not being pumped into the chamber? Everything else
seems to be working, so I am rather confused. Thanks. g

---------------------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Thu May 19 20:15:36 2005



From: Angela V. Klaus :      avklaus-at-amnh.org
Date: Thu, 19 May 2005 21:13:37 -0400 (EDT)
Subject: [Microscopy] Denatured vs. Absolute Ethanol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

A new person in my lab (new to biology) ordered denatured ethyl alcohol
instead of absolute EtOH for routine tissue processing for SEM. Just out
of curiosity, has anyone ever inadvertently used denatured ethanol for
dehydration and CPD for SEM? I'll probably use what we have for a
cleaning solvent or give it to the histo lab, but I'm curious if this has
come up in any of your labs.

The listserv archive has a brief thread on the use of denatured EtOH for
dehydration and embedment of paper in Spurr's resin.

Many thanks,

Angela

--
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-796-5977
Fax: 212-496-3480



From MicroscopyL-request-at-ns.microscopy.com Fri May 20 06:01:21 2005



From: Massimo :      andromeda_tm-at-libero.it
Date: Fri, 20 May 2005 13:00:07 +0200
Subject: [Microscopy] An adapter for Digital Camera Nikon Coolpix 4800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Torino 20 May 2005
(Italy)

Hi all,
I have bought a Digital Camera Nikon Coolpix 4800 and I am going to substitute the standard Zenit camera on the Lomo trinocular head MFN-11 (alias HF-30) of my microscope with it.
I don’t know which adapter I need.
Could someone give me some suggestions about?
Thank you.
With my Best Regards,

Massimo

--------------------------
"Il faut garder sa liberté
d'esprit et croire que dans
la nature l'absurde suivant
nos théories n'est pas
toujours impossible."
(Claude Bernard)




____________________________________________________________
6X velocizzare la tua navigazione a 56k? 6X Web Accelerator di Libero!
Scaricalo su INTERNET GRATIS 6X http://www.libero.it





From MicroscopyL-request-at-ns.microscopy.com Fri May 20 10:13:28 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 20 May 2005 11:09:34 -0400
Subject: [Microscopy] Re: Life and Dry Blood Analyis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Divna Unipan wrote:

} I would like to take Life and Dry Blood Analysis course from somebody who teach Dr.Enderlein's concept of } microscopie. Do you know anybody that teach this course?

} Sincerely,
} Divna Unipan, ND

Dear Divna:

Not seeing any other responses to your posting .......... I think
the reason no one has replied it that "Life and Dry Blood Analyisis" is
not held in much esteem by scientists. While I am reluctant to say that
it is quackery, it certainly does not have a track record of scientific
data and evidence to support it. While one can learn much from the study
of blood chemistry and morphology, there are limits, as there are in any
field.
I suggest that you use google.com to search for the information you
seek.

Geoff

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From MicroscopyL-request-at-ns.microscopy.com Fri May 20 15:24:24 2005



From: Richard Edelmann :      edelmare-at-muohio.edu
Date: Fri, 20 May 2005 16:23:34 -0400
Subject: [Microscopy] Hard-disk surface roughness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

O.k., folks before we try and re-build the wheel, has anyone looked at, or
measured the surface roughness of a (modern) computer harddisk? How
rough / flat are they?

We're looking for a flat surface to so some electro-chemistry work on, and
ITO glass is just too rough, and the crystal orientation of a silicon crystal
interferes with the work.

Thanks!


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


From MicroscopyL-request-at-ns.microscopy.com Fri May 20 16:06:33 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Fri, 20 May 2005 16:05:34 -0500
Subject: [Microscopy] Re: Hard-disk surface roughness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Richard

Two approaches: The guys at Zygo have been doing this for years very neatly with scanning white light interferometry. (www.zygo.com).

Alternatively, my colleague, Kim Kangasniemi, has been looking at this problem with an AFM. Another advantage: you can do electrical measurements with the same tool. You can reach him at kim-at-nt-america.com

Hope this is helpful,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.




At 03:23 PM 5/20/2005, Richard Edelmann wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Fri May 20 23:49:22 2005



From: root-at-host128.ipowerweb.com
Date: Sat, 21 May 2005 04:22:40 GMT
Subject: [Microscopy] 4,8 Mill. Osteuropaeer durch Fischer-Volmer Erlass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lese selbst:
http://www.npd.de/npd_info/deutschland/2005/d0405-13.html

Neue Dokumente:
http://www.rp-online.de/public/article/nachrichten/politik/deutschland/87647

Botschafter in Kiew beschwerte sich noch 2004:
http://www.rp-online.de/public/article/nachrichten/politik/deutschland/85735

Traumziel Deutschland:
http://www.berlinonline.de/berliner-zeitung/archiv/.bin/dump.fcgi/2004/1221/politik/0009/index.html

Kanzler erleichtert Visaverfahren für Golfstaaten:
http://www.spiegel.de/spiegel/vorab/0,1518,349262,00.html

Ohne Deutsch nach Deutschland:
http://www.aufenthaltstitel.de/zuwg/0618.html

Vorbildliche Aktion:
http://www.npd.de/npd_info/deutschland/2004/d1204-24.html


From MicroscopyL-request-at-ns.microscopy.com Sat May 21 03:25:19 2005



From: Alberto Diaspro :      diaspro-at-fisica.unige.it
Date: Sat, 21 May 2005 10:24:31 +0200
Subject: [Microscopy] Research programs at LAMBS-MicroScoBio, Diaspro Lab, Genoa Italy (ABDUS SALAM ICTP)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In 2005-2006, the Abdus Salam International Centre for Theoretical
Physics (ICTP), in the framework of a programme sponsored by the
Italian Government and Italian research institutions, is offering
fellowships to scientists from developing countries (Central and
Eastern Europe included) in the field of

Physics applied to Neurobiology.

The duration of the fellowships is between 6 and 12 months and the
monthly grant is from Euro 1,250 onwards. Limited funds are available
for travel. Health insurance coverage is provided to scientists and
accompanying family members. Family allowance is granted for dependant
family members.

All applications and correspondence should be addressed to the:


ICTP PROGRAMME FOR TRAINING AND RESEARCH IN ITALIAN LABORATORIES

the Abdus Salam INTERNATIONAL CENTRE FOR THEORETICAL PHYSICS

Strada Costiera 11

34014 Trieste, Italy

Telephone: +39 40 2240553 - 2240556

Telefax: +39 40 2240558

E-mail: itlabs-at-ictp. it

Web-page: http://www.ictp.trieste.it/www_users/ItaLab/index.html



The applicants should submit the Request for Participation Form in two
copies either typed or in dark, legible print. Please ensure that
additional requested documents are attached.

Applications should be submitted by 31 July 2005



LAMBS - Laboratorio Avanzato di Microscopia, Bioimmagini e
Spettroscopia, Centro di Ricerca MicroScoBio, Dipartimento di Fisica,
Università di Genova

(LAMBS - Laboratory for Advanced Microscopy, Bioimaging and
Spectroscopy, MicroScoBio Research Centre, Department of Physics,
University of Genoa)

(http://www.lambs.it)

GENOVA

Activity leader: A. Diaspro

(diaspro-at-fisica.unige.it)

Scientists involved: P. Bianchini, V. Caorsi, S. Krol, R. Magrassi, D.
Mazza, G. Vicidomini, P. Ramoino, C. Usai
Minimum duration of stay: 12 months

Main research themes:

Membrane internalization and intracellular trafficking.
Neurotransmitter synthesis and release.
Membrane excitability modulation by neurotransmitter.
Characterization of homo- and heterotransporters in synaptosomes and
gliosomes from mammalian cells.

Deconvolution techniques applied to 3D cellular networks using confocal
and two-photon fluorescence microscopy for cell imaging. Single
molecule fluorescence 3D tracking in cells and tissue; caging and
uncaging under multiphoton excitation and tissutal nanoscalpel;
fluorescence photoactivation.

F techniques (FRAP, FRET, FLIM, FCS) for molecular and cellular
imaging. Nano-bio-robots (hybrid polyelectrolyte-living cells systems).



Equipment available:
Multiphoton system based on Leica SP2 AOBS confocal spectral microscope
and Chameleon-XR tunable ultrafast Ti-Sapphire laser.

Two-photon excitation architecture based on Nikon PCM2000 Confocal
scanning head and Tsunami-Millennia ultrafast Ti-Sapphire laser system.

Fluorescence lifetime module Becker and Hickl.

FCS, frequency domain lifetime ISS, Urbana Champaign.

Confocal spectral system Nikon C1-sp1.

SHG (Second Harmonic Generation) set-up.

Single molecule detection set-up.

Microscope live cell imaging incubator.

Calcium imaging fluorescence architecture.

Cell culturing facilities (Protozoan, mammalian and yeast cells).

Chemical and biochemical preparation facility.

Nanofabrication facility for polyelectrolyte based hybrid systems.

Three-dimensional deconvolution workstation, www.powermicroscope.com
facility.


Link to: http://www.ictp.trieste.it/www_users/ItaLab/neurobiology.html


------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------
[...] Dance with wolves and count the stars, including the
unseen...(L.Ferlinghetti, Challenges to young poet, 2001)

------------------------------------------------------------------------
------------------------------------------------------------------------
-----------------------------------------------------------
Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department
of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309; URL:
http://www.lambs.it
http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html

------------------------------------------------------------------------
------------------------------------------------------------------------
------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Sat May 21 08:30:59 2005



From: Don Chernoff at ASM :      donc-at-asmicro.com
Date: Sat, 21 May 2005 08:10:55 -0500
Subject: [Microscopy] [a] Hard-disk surface roughness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard Edelmann asked about the roughness of a hard disk surface, as he
considers its suitability for electrochemistry.
The roughness of hard disk media is generally very small in the data region,
probably Ra { 0.2 nm. In the head parking region, the roughness is
deliberately increased by patterning the surface, so that the head stiction
is reduced. In both regions, AFM is commonly used to measure the
microroughness and also the geometric parameters of the shallow bumps.

Roughness is only part of the story. The materials of construction should
be considered, for example:
superpolished Aluminum substrate/CoCr or similar alloy (magnetic
layer)/diamond-like carbon/fluorinated lubricant.
Other substrates, such as glass and Silicon may be used. Other thin film
coatings may be used. Because of the composite nature of a hard disk, you
may find that it complicates the electrochemistry.

Therefore, from a chemical standpoint, Si might actually be better. Be
aware that you can choose different crystal orientations. Perhaps one will
be compatible with your system.

I hope this is helpful.
regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]


----- Original Message -----
} From: Richard Edelmann
To: microscopy-at-microscopy.com
Sent: Friday, May 20, 2005 3:23 PM

O.k., folks before we try and re-build the wheel, has anyone looked at, or
measured the surface roughness of a (modern) computer harddisk? How
rough / flat are they?

We're looking for a flat surface to so some electro-chemistry work on, and
ITO glass is just too rough, and the crystal orientation of a silicon
crystal
interferes with the work.

Thanks!


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."



From MicroscopyL-request-at-ns.microscopy.com Sat May 21 09:56:42 2005



From: Richard :      richard-at-torland.demon.co.uk
Date: Sat, 21 May 2005 15:57:16 +0100
Subject: [Microscopy] Re: RE: viaWWW: stuck film chamber door

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello

you can tell if the camera chamber is leaked by looking at the o ring under
the window to the viewing chamber, When at vacuum the "flat" surface is a
few mill across, as the air goes in this "flat" part gets thinner until it
is just a thin conact line at atmospheric,

regards

Richard Hey
Jeol (UK) Ltd





At 20:59 19/05/2005 -0400, Scott Walck wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From MicroscopyL-request-at-ns.microscopy.com Sat May 21 18:56:40 2005



From: kyhour-at-yahoo.com (by way of MicroscopyListserver)
Date: Sat, 21 May 2005 18:55:58 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: science project to study cesium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kyhour-at-yahoo.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, May 21, 2005 at 07:33:09
---------------------------------------------------------------------------

Email: kyhour-at-yahoo.com
Name: Michelle Hour

Organization: Central Virginia Governer School

Education: 9-12th Grade High School

Location: Lynchburg, Va, 24503

Question: Hi,

My daughter, Michelle, has been working on a science project to study cesium chloride microstructure change due to cyclic heating and cooling above and below transition temeprature. She has used SEM to demonstrate that there are changes. To further her study, she need to have TEM work done on two samples to determine what the changes are. Could you tell me where she can find institutions supporting this type of request? Thank you in advance.

Kevin Hour

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat May 21 19:09:39 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 21 May 2005 17:09:01 -0700
Subject: [Microscopy] Iodine crystals source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone know of good sources for iodine crystals
for use in ion beam etching systems?

gary g.



From MicroscopyL-request-at-ns.microscopy.com Sat May 21 19:09:52 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 21 May 2005 17:09:14 -0700
Subject: [Microscopy] Re: Hard-disk surface roughness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Last time I looked, the disk had globs/nodules that
were in the 65nm range. There were also some concentric rings
that were "dug" into the surface.

For a really flat surface, you might try a quartz/chrome
integrated circuit photo mask blank. These are .25" thick
and are coated with a thin layer of chrome. The glass and
final finish are very flat. To get a small piece, you will
likely need a diamond wire saw. For a small amount of material,
a scrap plate ought to suffice.

gary g.


At 01:23 PM 5/20/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Sun May 22 11:25:53 2005



From: ted dunn :      drteddunne-at-yahoo.com
Date: Sun, 22 May 2005 09:25:12 -0700 (PDT)
Subject: [Microscopy] Re: Re: RE: viaWWW: stuck film chamber door

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In response to your question "Should I force it [the
door]".....There is hardly ever a situation with
electron microscopes in general where force is a good
idea whether a component is under vacuum or not. It is
definitely an action of last resort and maybe not even
then!
Good luck with the problem.
Ted Dunn
The EMscope Company Ltd.


Email: beagleyg-at-alma.edu
} Name: gwyneth beagley
}
} Organization: department of psychology alma college
}
} Title-Subject: [Microscopy] [Filtered] stuck film
chamber door
}
} Question: Has anyone else experienced a "stuck" door
on a film chamber of a
} JEOL 1200 EX? Should I force it? The latch turns; is
the failure to open due
} to too much vacuum or air not being pumped into the
chamber? Everything else
} seems to be working, so I am rather confused. Thanks.
g
}






Yahoo! Mail
Stay connected, organized, and protected. Take the tour:
http://tour.mail.yahoo.com/mailtour.html



From MicroscopyL-request-at-ns.microscopy.com Sun May 22 13:00:00 2005



From: Valery Ray :      vray-at-partbeamsystech.com
Date: Sun, 22 May 2005 10:59:18 -0700 (PDT)
Subject: [Microscopy] Re: Iodine crystals source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,

Besides of obvious source of consumables from OEM of
your FIB, try Alfa-Aesar (www.alfa.com):

Item #10619 Iodine, resublimed crystals, Puratronic®,
99.9985% (metals basis), I believe you should be able
to get 100g for under US$80. Be careful while
re-loading crucible!

Cheers,

Valery Ray

Particle Beam Systems
& Technology
www.partbeamsystech.com


From MicroscopyL-request-at-ns.microscopy.com Sun May 22 15:18:52 2005



From: Scott Walck :      swalck-at-comcast.net
Date: Sun, 22 May 2005 16:15:22 -0400
Subject: [Microscopy] Iodine crystals source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You can buy iodine crystals from Fisher Scientific. That's what I once used
in an old Gatan Duomill that was equipped with the iodine capable guns. I
also bought the charcoal for the iodine trap between the turbo pump and the
mechanical pump from a pet store. The price was a little more reasonable.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499
eMail: Walck-at-SouthBayTech.com

Temporary Pittsburgh Area Phone Number:
412-492-8127

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Saturday, May 21, 2005 8:09 PM
To: MSA listserver

Does anyone know of good sources for iodine crystals
for use in ion beam etching systems?

gary g.






From MicroscopyL-request-at-ns.microscopy.com Sun May 22 20:07:17 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 23 May 2005 13:07:21 +1200
Subject: [Microscopy] Used JSM-840???

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Anyone out there got a good used JSM 840 for sale?

cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Mon May 23 02:47:47 2005



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Mon, 23 May 2005 08:47:17 +0100
Subject: [Microscopy] Hard-disk surface roughness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Why not just get a silicon wafer which has been oxidised? A thin layer of oxide shouldn't increase roughness too much.

I have all sorts of 'bits' in my scrap bin, but I'm sure you can find better sources..

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


-----Original Message-----
} From: Richard Edelmann [mailto:edelmare-at-MUOhio.edu]
Sent: 20 May 2005 21:24
To: microscopy-at-microscopy.com

O.k., folks before we try and re-build the wheel, has anyone looked at, or
measured the surface roughness of a (modern) computer harddisk? How
rough / flat are they?

We're looking for a flat surface to so some electro-chemistry work on, and
ITO glass is just too rough, and the crystal orientation of a silicon crystal
interferes with the work.

Thanks!


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."


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From MicroscopyL-request-at-ns.microscopy.com Mon May 23 05:34:16 2005



From: Niko Hellsten :      niko.hellsten-at-gmail.com
Date: Mon, 23 May 2005 12:33:34 +0200
Subject: [Microscopy] Ion thinning question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Just a general question regarding ion thinning. Has anybody ever heard
of or used any type of marking method to localize certain area of
interest? I'm currently (still) working with silicon cross-section
samples. After mechanic polishing and making a mirror surface for the
final ion thinning, it would help if there was some way of localizing
where to make the hole.

To do the ion thinning, the sandwitch is fixed on a titanium fixation
ring (BAL-TEC) and thickness of the sandwitch is 1mm. So, if there was
some kind of method for marking the sample before thinning, it would
help. Anybody out there ever had this kind of problem/situation?

- Niko Hellstén
ENSPG/LMGP
Grenoble, France

P.S. I know that most people would probably use FIB in my case but
that's not an option here.



From MicroscopyL-request-at-ns.microscopy.com Mon May 23 08:57:50 2005



From: Hank Beebe :      hbeebe-at-rjlg.com
Date: Mon, 23 May 2005 10:00:37 -0400
Subject: [Microscopy] Looking for PSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

RJLee Group Inc, is looking for used PSEMs
The PSEMs would have been manufactured by RJLee Instruments or ASPEX.
They are the same company with a name change.

Please contact me at the below address
Thank You

Hank Beebe

Manager Instrument Services
hbeebe-at-rjlg.com
RJLee Group, Inc.
350 Hochberg Rd.
Monroeville, PA 15146
(724) 325-1776 voice
(724) 733-1799 fax
(412) 897-8402 cell





From MicroscopyL-request-at-ns.microscopy.com Mon May 23 08:59:45 2005



From: Hank Beebe :      hbeebe-at-rjlg.com
Date: Mon, 23 May 2005 10:02:32 -0400
Subject: [Microscopy] Looking for PSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

RJLee Group Inc, is looking for used PSEMs
The PSEMs would have been manufactured by RJLee Instruments or ASPEX.
They are the same company with a name change.

Please contact me at the below address
Thank You

Hank Beebe

Manager Instrument Services
hbeebe-at-rjlg.com
RJLee Group, Inc.
350 Hochberg Rd.
Monroeville, PA 15146
(724) 325-1776 voice
(724) 733-1799 fax
(412) 897-8402 cell





From MicroscopyL-request-at-ns.microscopy.com Mon May 23 10:34:16 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Mon, 23 May 2005 13:02:56 -0230
Subject: [Microscopy] OM: adapting larger CCDs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was hoping that someone had been successful in adapting a digital camera
that has a larger CCD (or CMOS) sensor to their optical microscope. That
is, I am intrigued with the dynamic range and lesser noise as offered by
modern dSLR cameras, such as the Canon 20D or Nikon D70. However, if I try
to calculate which C-mount is appropriate for FOV=22mm I come up short
because the sensor diagonals are larger (e.g., ~26mm).

Alternatively, we consider spending a bit more money for the "full frame"
dSLRs (Kodak DCS, Canon EOS-1D Mk II), and adapting as a traditional 35mm,
but I'd still appreciate your comments and suggestions.

TIA & cheerios ... michael shaffer :o)
Avalon Peninsula, Newfoundland
www.micro-investigations.com (in progress)



From MicroscopyL-request-at-ns.microscopy.com Mon May 23 12:19:42 2005



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 23 May 2005 10:12:19 -0700
Subject: [Microscopy] bacterial flagella

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

I am trying to help a guy see flagella on cholera bacteria and we are
having some trouble.

Using standard techniques with 2% UA in water as a neg stain, we can see
flagella from wild type cultures clearly.

We are having problems with some mutant strains. These mutants produce a
lot of unidentified polysacchride that makes the cell clump together. Also,
when the UA goes on there is some kind of precipitate that forms obscuring
the whole thing. With no UA, there are fern frond like things all over the
place, but no bacteria.

The mutants are gowing in LB culture medium or on plates. So far, I haven't
been able to see anything that looks like a bacterium, just all the other
junk.

Any suggestions about where to go next?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From MicroscopyL-request-at-ns.microscopy.com Mon May 23 13:26:08 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Mon, 23 May 2005 13:25:09 -0500
Subject: [Microscopy] Re: bacterial flagella

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Jonathan,

This is another one of those "CytoViva" applications. I'd recommend that you go to their website (www.cytoviva.com) and take a look. I think that you may be able to get away without any staining at all and see much more with this new technique.

Reference:
Foster, B. "Focus on Microscopy: a Technique for Imaging Live Cell Interactions and Mechanisms," Am Lab, Nov 2004.

By the way, you can get a PDF on line by going to www.iscpubs.com. Click on "Articles" (left menu), then ask for "2004" and Foster. You'll see it listed there.

Hope this was helpful.

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts.



At 12:12 PM 5/23/2005, Jon Krupp wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Mon May 23 18:23:30 2005



From: hallw-at-creighton.edu (by way of MicroscopyListserver)
Date: Mon, 23 May 2005 18:22:47 -0500
Subject: [Microscopy] viaWWW: Advice needed on Confocal Experiment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (hallw-at-creighton.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, May 23, 2005 at 10:59:12
---------------------------------------------------------------------------

Email: hallw-at-creighton.edu
Name: Richard Hallworth

Organization: Creighton University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: WE are using a Zeiss LSM-510 confocal and would like to do an unusual ROI experiment. Specifically, we would like to bleach everywhere EXCEPT some closed ROIs. Does anyone know how to do this?

Thanks

Rick Hallworth


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon May 23 20:36:50 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 24 May 2005 13:36:55 +1200
Subject: [Microscopy] Detector pump 'n' bake temperature?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

May have your opinions about the optimum temperature to which to heat the interior of
the Dewar when re-evacuating an EDS detector?

cheers

rtch


--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Mon May 23 22:38:01 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 23 May 2005 20:37:20 -0700
Subject: [Microscopy] Denton Desk II coater failure solutions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

hello all:

I have a relatively new Denton Desk II coater (~2years old)
that has failed in an odd manner. It will generate a plasma
but will shut down if the current is above 25-28mA. Vacuum
is steady at 95-100mT.

I've replaced the target and bought new magnets and pump
mist filter to no avail. Starting the unit in coating
mode surges the current and then shuts off the HV.
The only way to get a plasma is to start from low current
and work up to 20mA or so. Even at this setting, and with
a Pt target, the coating is very coarse.

Short of sending the whole thing back East, are there any
ideas out there about what could be causing this problem?
Shipping this unit back is not a fascinating option
or prospect. At this point, this is what Denton is
suggesting.

Coaters are a necessity but seem to be a real pain.
I am energized to focus on the HV area of this coater
and any new replacement. This seems to be a very vulnerable
area for coaters...IMO.

gary g.



From MicroscopyL-request-at-ns.microscopy.com Tue May 24 08:03:30 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 24 May 2005 09:02:27 -0400
Subject: [Microscopy] Re: viaWWW: Advice needed on Confocal Experiment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Rick,
The drawing of the ROIs gets a little tricky, but think in "negative
contrast", that is define your ROIs as all the other stuff, so that
your true regions are left out of the scan.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue May 24 08:13:38 2005



From: Lehman, Ann R :      Ann.Lehman-at-trincoll.edu
Date: Tue, 24 May 2005 09:14:33 -0400
Subject: [Microscopy] anaesthesia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who replied with ideas about alternative anaesthetic
agents. This seems to be a common problem, but the suggestions offered
were just what I needed. The List rarely disappoints! (Thanks, Nestor.)

Ann Hein Lehman
Assistant Director
Electron Microscopy Facility
Trinity College
300 Summit Street
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman




From MicroscopyL-request-at-ns.microscopy.com Tue May 24 08:29:15 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Tue, 24 May 2005 08:28:18 -0500
Subject: [Microscopy] Re: Denton Desk II coater failure solutions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary,

When I've had this problem it's been because of one of two things:
1) The target or magnet mounting has vibrated loose (doesn't have to
be much), allowing them to contact and short. Cure: check mounting of
target holder and magnetrons, etc., and tighten being very careful
not to crack any epoxy or other sealant around the HV feedthrough. Oh
.. 1b ... check for checks in such sealants. Reseal if there are any
with high-temperature epoxy.
2a) The sputter head has accumulated either oil or other substances
from the rotary pump or samples, especially conductive paint used to
stick the samples to the stub (you do wait until the C or Ag paint is
*completely* dry before putting the sample in the coater, yes?) or,
2b} Sputtered metal has accumulated at the base of the target mount.
Either can cause shorting and failure to coat. Cure: disassemble and
clean like mad.

Phil

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm


From MicroscopyL-request-at-ns.microscopy.com Tue May 24 09:54:29 2005



From: Ciaburri, Diane A :      Diane.Ciaburri-at-gd-ais.com
Date: Tue, 24 May 2005 10:51:44 -0400
Subject: [Microscopy] Ultrasonic Cleaners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our EHS staff just informed me that we shouldn't be using solvents in
our ultrasonic cleaner. Sure enough, when I read the label, it says not
to use sovents with a flash point below 100C. We've used isopropyl
alcohol (flash point 54C) for years (with the hood on) and never had a
problem. Is there such a thing as an ultrasonic cleaner approved for
low flashpoint solvents? What is the standard procedure?


Diane Ciaburri
General Dynamics
Pittsfield MA 01201
(413)494-3430



From MicroscopyL-request-at-ns.microscopy.com Tue May 24 11:07:18 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Tue, 24 May 2005 09:06:36 -0700 (PDT)
Subject: [Microscopy] Re: Ultrasonic Cleaners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's not so much the solvents as the solvents
sonicated for a long time. I use all kinds of
solvents in the ultrasonic cleaner, e.g., alcohol,
acetone, mineral spirits.

If left unattended for a long while, the solvents
become heated to the point where a fire may start. I
believe this may happen if the bath is left to
sonicate for over an hour. This is a real danger if
the operator leaves the bath unattended and forgets
about it. I remember times where I left the bath
sonicating for an hour and the sample was so heated
that the alcohol solution was boiling.

Your only saving grace with EHS may be if your
apparatus has a timer that you can set. It also
depends on whether they banned ANY presence of
volatiles in the unit. I often fill the unit with
water, then place a beaker filled with solvent to
clean the part. But this only reduces the danger when
compared with filling the entire bath with solvent.

Stu Smalinskas, P.E.
Metallurgist
SKF
Plymouth, Michigan

--- "Ciaburri, Diane A" {Diane.Ciaburri-at-gd-ais.com}
wrote:
}
} Our EHS staff just informed me that we shouldn't be
} using solvents in
} our ultrasonic cleaner. Sure enough, when I read
} the label, it says not
} to use sovents with a flash point below 100C. We've
} used isopropyl
} alcohol (flash point 54C) for years (with the hood
} on) and never had a
} problem. Is there such a thing as an ultrasonic
} cleaner approved for
} low flashpoint solvents? What is the standard
} procedure?
}
}
} Diane Ciaburri
} General Dynamics
} Pittsfield MA 01201
} (413)494-3430
}




__________________________________
Do you Yahoo!?
Yahoo! Small Business - Try our new Resources site
http://smallbusiness.yahoo.com/resources/


From MicroscopyL-request-at-ns.microscopy.com Tue May 24 15:31:24 2005



From: Beth Richardson :      beth-at-plantbio.uga.edu
Date: Tue, 24 May 2005 16:30:39 -0400
Subject: [Microscopy] vibratome calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
Can anyone help with this request (see forwarded message below)?
Please reply directly to Mark Karadsheh mkaradsh-at-umich.edu.
thanks,
Beth

Begin forwarded message:

} Name: Mark Karadsheh
} E-Mail: mkaradsh-at-umich.edu
} Message:
}
} My lab has an Oxford vibratome and needs to be recalibrated. When
} searching ont the internet for possibilities, I ran across your post
} on the Microscopy ListServer. I know this was a long time ago, but do
} you have any ideas on possible locations to look. Thanks so much.
}
} Mark Karadsheh
}
}

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***




From MicroscopyL-request-at-ns.microscopy.com Tue May 24 20:44:39 2005



From: Anthony Garratt-Reed :      tonygr-at-MIT.EDU
Date: Tue, 24 May 2005 21:41:23 -0400
Subject: [Microscopy] Re: Detector pump 'n' bake temperature?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Hi
}
} May have your opinions about the optimum temperature to which to heat the
} interior of
} the Dewar when re-evacuating an EDS detector?
}
} cheers
}
} rtch

I have tried any number of different techniques, including the use of
boiling water, an extended pumping at room temperature, a quick warm-up,
pump and cool down, etc., I can't say I've noticed any great difference
in the final result.

Tony Garratt-Reed


*********************************************
Anthony J. Garratt-Reed, M.A., D.Phil
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
*********************************************
Phone: (617) 253-4622
Fax: (617) 258-6479
*********************************************



From MicroscopyL-request-at-ns.microscopy.com Tue May 24 21:34:17 2005



From: mcarter-at-csuhayward.edu (by way of MicroscopyListserver)
Date: Tue, 24 May 2005 21:33:34 -0500
Subject: [Microscopy] viaWWW: Surplus Equipment from Cal State Hayward

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mcarter-at-csuhayward.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, May 24, 2005 at 10:42:34
---------------------------------------------------------------------------

Email: mcarter-at-csuhayward.edu
Name: Melissa

Organization: Cal State Hayward

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Hello All, The sad news is our lab is having to shut down to make room for other technological instruments... The good news is that we have a bunch of equipment we need to get into some qualified & loving hands. We have Pelco sputter and carbom coaters, a relatively new Polaron 3000 jumbo CPD, Tousimis CPD, one new Leica Knife Maker, some older LBK knife makers, a Reichert ultramicrotome (still works but also good for parts???) a Pelco 3450 Lab. Microwave Processor with load cooler..... We may also have a Philips XL40/ IXRF Systems X-ray analysis/ Backscatter/ CCD. with new chiller available... waiting to see if a local college is interested... Does anyone have any suggestions for where to list these items for sale? Or is anyone interested? Please let me know either via the list or you can contact me at mcarter-at-csuhayward.edu 510-885-3527

Thanks for any help,

Melissa

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue May 24 21:35:13 2005



From: km602223-at-comcast.net (by way of MicroscopyListserver)
Date: Tue, 24 May 2005 21:34:30 -0500
Subject: [Microscopy] viaWWW: Fluorescence microscopy Lamps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (km602223-at-comcast.net) from http://microscopy.com/MLFormMail.html on Tuesday, May 24, 2005 at 19:34:08
---------------------------------------------------------------------------

Email: km602223-at-comcast.net
Name: Kathleen McMillan

Title-Subject: [Microscopy] [Filtered] Fluorescence microscopy

Question: Hello,

I bought an old Nikon Fluophot microscope which I am attempting to get in good working order. I believe the optics are in good condition and I purchased some high quality objectives. It came with the original epi fluorescence lamp house with a 200 W mercury lamp. The problem is, there is no power supply for the lamp house. I tried Ludl Electronics, and while they make electrical adaptors for older lamphouses, their supplies are designed only for lamps 100 W or below.

Does anyone know where I might find a suitable power supply for this lamphouse? If it is my only practical choice, I could use a power supply that works only with the 200 W Hg lamp. However, I would prefer flexibility to use either mercury or xenon lamps.

Alternatively, is it possible and would it make sense to remove the original lamphouse and use a fiber coupled light source instead? There are some reasonably priced fiber coupled complete arc lamp systems on the secondary market.

Any advice or suggestions would be appreciated. I am a phyical chemist with some knowledge of optics but little experience with microscopes.

Thanks,

Kathleen

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed May 25 01:23:45 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 24 May 2005 23:22:52 -0700
Subject: [Microscopy] Ultrasonic Cleaners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Some EHS staff are significantly either uninformed or misinformed about
a lot of things. I have had EHS staff demand that JEOL redesign our
2010F so that the anticontamination device dewar and the EDX dewar were
in different positions to make them easier to fill. Basically, he
thought he knew more about TEM design than JEOL did. He also insisted
that filling a dewar to overflowing (the only way to tell if it is
full)was reckless. To the end, he insisted that incidental contact with
LN was an extreme hazard. Well, he was really a nice guy, and meant
well, but.......So if your EHS expert is cut from the same cloth, don't
worry too much about using solvents in an ultrasonic cleaner. I've been
doing it for ~33 years, and never started a fire.

John Mardinly
Intel

These are the opinions of the author, and not the opinion of Intel
corporation.


-----Original Message-----
} From: Ciaburri, Diane A [mailto:Diane.Ciaburri-at-gd-ais.com]
Sent: Tuesday, May 24, 2005 7:52 AM
To: microscopy-at-microscopy.com

Our EHS staff just informed me that we shouldn't be using solvents in
our ultrasonic cleaner. Sure enough, when I read the label, it says not
to use sovents with a flash point below 100C. We've used isopropyl
alcohol (flash point 54C) for years (with the hood on) and never had a
problem. Is there such a thing as an ultrasonic cleaner approved for
low flashpoint solvents? What is the standard procedure?


Diane Ciaburri
General Dynamics
Pittsfield MA 01201
(413)494-3430





From MicroscopyL-request-at-ns.microscopy.com Wed May 25 06:15:05 2005



From: Patricia Scallion :      PSCALLIO-at-DAL.CA
Date: Wed, 25 May 2005 08:14:24 -0300
Subject: [Microscopy] hydrogel mesh

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I have a student who wishes to "measure the mesh size of a gelatin/maltodextrin
hydrogel crosslinked with genipin. The sample is mostly water and has the
texture of jello."

We have a Hitachi S-4700 FE-SEM, and are wondering what is the best way to
prepare his samples to get the results that he wants. We don't have a CPD, but
do have access to a gold sputter coater.

Thanks for all suggestions.

Pat
Research Technician
SEM-FIB Facility
Institute for Research in Materials
Dalhousie University
(902) 494-1258








From MicroscopyL-request-at-ns.microscopy.com Wed May 25 08:40:14 2005



From: pgrover :      pgrover-at-bilbo.bio.purdue.edu
Date: Wed, 25 May 2005 08:39:37 -0500
Subject: [Microscopy] re: Surplus Equipment from Cal State Hayward

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hey Melissa,

Google 'used lab equipment' and you will find a bozillion places that will
buy your items or sell them for you. I have been purchasing as well as
selling instruments on these sites for many years, and have had nothing but
good luck. The results you get may vary, but I wish you the same good luck,
godspeed, and, ah, blah blah woof woof ;o)

Paul

Question: Hello All, The sad news is our lab is having to shut down to make
room for other technological instruments... The good news is that we have a
bunch of equipment we need to get into some qualified & loving hands. We
have Pelco sputter and carbom coaters, a relatively new Polaron 3000 jumbo
CPD, Tousimis CPD, one new Leica Knife Maker, some older LBK knife makers,
a Reichert ultramicrotome (still works but also good for parts???) a Pelco
3450 Lab. Microwave Processor with load cooler..... We may also have a
Philips XL40/ IXRF Systems X-ray analysis/ Backscatter/ CCD. with new
chiller available... waiting to see if a local college is interested... Does
anyone have any suggestions for where to list these items for sale? Or is
anyone interested? Please let me know either via the list or you can
contact me at mcarter-at-csuhayward.edu 510-885-3527

Thanks for any help,

Melissa

---------------------------------------------------------------------
Art is long, and critics are the insects of a day. - Randall Jarrell





From MicroscopyL-request-at-ns.microscopy.com Wed May 25 08:47:33 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Wed, 25 May 2005 08:46:50 -0500
Subject: [Microscopy] Re: hydrogel mesh

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Pat,

The best way to do this is with cryoSEM. You don't mention if you've
got the equipment for this, but I suspect not -- most materials
facilities don't.
The next best choice is freeze-drying. Be sure to freeze small enough
samples. If you don't have access to a high pressure freezer, then
another good way is plunge-freezing into slush nitrogen, made by
pulling a vacuum (with a *high* capacity pump) on LN2. Then vacuum
sublimate starting about -90 deg C. Leave until the pressure is {~6
microns Hg (or whatever the vapor pressure of water is at the
temperature and vacuum you use. Vacuum should be ~10^-5 torr -- diff
pump or big rotary pump range. Make sure the vacuum system has a big
throat and short, direct path to the pump, or better, a LN2 cold
trap. Once you get below the vapor pressure (V.P.) of water, slowly
raise the temperature, stopping if (when) the pressure goes above the
V.P. of water at that temp. Continue to about -60 deg C. Around here,
the water of hydration and other bound water will start come off. Be
careful, this is where most specimen collapse happens. Once the
pressure is again below the V.P. of water at this temperature,
continue until about -40 deg. C. Pause if needed. Work you way up to
-20 and let warm. This will likely take 24 to 48 hours.
CPD can be useful, if done carefully and correctly and thoroughly and
ALL the water is gotten out *and* the ethanol doesn't affect the gel
*and* ALL of the EtOH is exchanged away in the CPD with enough cycles
of soaking and purging (meaning most manufacturers directions I've
seen are wrong). But cryoSEM is best, and freeze-drying next best for
true structure preservation of hydrogels.

Phil

} Hello,
} I have a student who wishes to "measure the mesh size of a
} gelatin/maltodextrin
} hydrogel crosslinked with genipin. The sample is mostly water and has the
} texture of jello."
}
} We have a Hitachi S-4700 FE-SEM, and are wondering what is the best way to
} prepare his samples to get the results that he wants. We don't have a CPD, but
} do have access to a gold sputter coater.
}
} Thanks for all suggestions.
}
} Pat
} Research Technician
} SEM-FIB Facility
} Institute for Research in Materials
} Dalhousie University
} (902) 494-1258

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm


From MicroscopyL-request-at-ns.microscopy.com Wed May 25 11:26:57 2005



From: Karl Hagglund :      hagglundk1-at-nku.edu
Date: Wed, 25 May 2005 12:26:15 -0400
Subject: [Microscopy] troubleshooting SEM emission Zeiss DSM 960A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am working on the reinstallation of a Zeiss DSM 960A (1992). There are
two apparent problems.

1. The microscope is working at low voltages ( {10kV), but the emission
current seems to short out at higher voltages. It seems to modulate
between a saturated and unsaturated condition constantly, as if its
ramping up the voltage, shorts, and then ramps up again. Occasionally,
coincident with the shorting, the entire system restarts itself. I have
removed and cleaned the Wehnelt and anode assembly and there are no
obvious tungsten hairs or buildup of residues that might physically be
causing the short. Likewise, the vacuum is stable, and I am not seeing
any indications of a leak near the gun and have no vacuum errors.

I have been able to make it work at 10 and 20kV, but have to saturate
very carefully, and even then the system does not appear to be stable.

2. The emission current is reading much higher than expectation. In an
unsaturated condition, the gauge reads approximately 370 milliAmps (I
believe it should read zero). When the filament is saturated it is
reading 460-480 uA (the manual suggests 80).


Does anyone out there have one of these microscopes and/or has someone
seen these problems before on this model or a similar one? Thanks in
advance for all your help. The list always seems to come through for
me.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu




From MicroscopyL-request-at-ns.microscopy.com Wed May 25 12:18:44 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wed, 25 May 2005 12:17:59 -0500
Subject: [Microscopy] Re: hydrogel mesh

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Pat,

The only way to handle SEM imaging of hydrogels is to use cryoSEM. Any
other preparation technique withdraws water which in turn changes the
structure. We have an appropriately equipped FESEM but I am sure you can
probably find one closer to home.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 5/25/05 6:14 AM, "Patricia Scallion" {PSCALLIO-at-DAL.CA} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Hello,
} I have a student who wishes to "measure the mesh size of a
} gelatin/maltodextrin
} hydrogel crosslinked with genipin. The sample is mostly water and has the
} texture of jello."
}
} We have a Hitachi S-4700 FE-SEM, and are wondering what is the best way to
} prepare his samples to get the results that he wants. We don't have a CPD, but
} do have access to a gold sputter coater.
}
} Thanks for all suggestions.
}
} Pat
} Research Technician
} SEM-FIB Facility
} Institute for Research in Materials
} Dalhousie University
} (902) 494-1258
}
}
}
}
}
}
}




From MicroscopyL-request-at-ns.microscopy.com Wed May 25 12:25:48 2005



From: Allan-Wojtas, Paula :      AllanWojtasP-at-agr.gc.ca
Date: Wed, 25 May 2005 13:23:54 -0400
Subject: [Microscopy] pacemakers and EM labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, all,
 
I believe there was a thread on this topic previously, but I wanted the most up to date information that exists, and quickly, so I thought I'd pose the question again.
 
We have a student at our Research Station who is scheduled for surgery tomorrow to implant a pacemaker. Although he is not working directly with the EMs or in the EM lab, he is working just down the hall. He is also planning a career in plant pathology, so he will probably be working in buildings which have EM labs and equipment.
 
My question is:
 
Is it safe for this student to be working down the hall from my lab - will the EM equipment interfere with his pacemaker? I imagine with all the shielding in the modern EMs that he should be OK, but it would be nice to know for sure so he will know when he comes back to work in a few days.
 
We are also asking this question about the other equipment (HPLCs, GCs, mass specs, etc.)
 
Thanks for your help with this.
 
Regards,
 
Paula.
 
Paula Allan-Wojtas
Research Scientist - Food Microstructure/Chercheure scientifique, microstructure des aliments
Food Safety and Quality Team/Salubrité et qualité des aliments
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
32 Main Street/ 32 rue Main
Kentville, Nova Scotia/Kentville, (Nouvelle-Écosse)
Canada B4N 1J5
Telephone/Téléphone: (902) 679-5566
Facsimile/Télécopieur: (902) 679-2311
 
allanwojtasp-at-agr.gc.ca
 
 

 



From MicroscopyL-request-at-ns.microscopy.com Wed May 25 12:43:07 2005



From: frank.karl-at-degussa.com
Date: Wed, 25 May 2005 13:42:06 -0400
Subject: [Microscopy] Re: pacemakers and EM labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





The last NMR I worked around (I walked in to the room on occasion) had a
warning up about pacemakers.

good luck!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
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in or attached to this transmission is STRICTLY PROHIBITED. If you have
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"Allan-Wojtas,
Paula" To: {microscopy-at-microscopy.com}
{AllanWojtasP-at-AGR cc:
.GC.CA} Subject: [Microscopy] pacemakers and EM labs

05/25/2005 01:23
PM







------------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi, all,
I believe there was a thread on this topic previously, but I wanted the
most up to date information that exists, and quickly, so I thought I'd pose
the question again.
We have a student at our Research Station who is scheduled for surgery
tomorrow to implant a pacemaker. Although he is not working directly with
the EMs or in the EM lab, he is working just down the hall. He is also
planning a career in plant pathology, so he will probably be working in
buildings which have EM labs and equipment.
My question is:
Is it safe for this student to be working down the hall from my lab - will
the EM equipment interfere with his pacemaker? I imagine with all the
shielding in the modern EMs that he should be OK, but it would be nice to
know for sure so he will know when he comes back to work in a few days.
We are also asking this question about the other equipment (HPLCs, GCs,
mass specs, etc.)
Thanks for your help with this.
Regards,
Paula.
Paula Allan-Wojtas
Research Scientist - Food Microstructure/Chercheure scientifique,
microstructure des aliments
Food Safety and Quality Team/Salubrité et qualité des aliments
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
32 Main Street/ 32 rue Main
Kentville, Nova Scotia/Kentville, (Nouvelle-Écosse)
Canada B4N 1J5
Telephone/Téléphone: (902) 679-5566
Facsimile/Télécopieur: (902) 679-2311
allanwojtasp-at-agr.gc.ca








From MicroscopyL-request-at-ns.microscopy.com Wed May 25 13:08:34 2005



From: Ralph Common :      Ralph.Common-at-hc.msu.edu
Date: Wed, 25 May 2005 14:07:33 -0400
Subject: [Microscopy] prions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A client wants to do TEM of prion containing tissue. I would like to know how other EM labs are handling such requests. If I decide to accept the project, what special precautions are needed? Do embedding wastes have to be separately handled and labeled? Is there any real risk after the tissue is in resin? I doubt there is much actual data on risk, but I would like to know how others feel about the issue.

Ralph Common
Michigan State University
Division of Human Pathology



From MicroscopyL-request-at-ns.microscopy.com Wed May 25 13:11:38 2005



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Wed, 25 May 2005 14:47:57 -0400
Subject: [Microscopy] vibrations in air cooled CCD cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I have not worked on the particular instrument that you describe but I have
an idea that may take you further.

To have such a high current when you switch on must indicate a leakage to
earth, the current has to go somewhere! If you switch off and then
disconnect the high voltage cable from the HT tank before switching on
again, does the problem go away? If so my best guess is the cable is your
problem! If the problem does not go away I am afraid the source is in the
HT tank.

I am just about to fly off to Brisbane, Australia, to run a course on
instrument maintenance so would be very interested in how you get on?
Please feel free to make contact again as I have other service technicians
helping me, the joint brains should be able to take you even further; I
hope!

Regards

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
} From: "Karl Hagglund" {hagglundk1-at-nku.edu}
To: {microscopy-at-microscopy.com}
Sent: Wednesday, May 25, 2005 5:26 PM

About a year ago we purchased a Roper CoolSnap HQ with fan in the camera
body. Problem is that we're getting a lot of vibrations. I installed a
switch in the wire to the fan. We may manually throw this switch to
disable the fan, but this is not a good solution because there is no
cooling airflow when the fan is off. (Examples are posted at
http://www.aecom.yu.edu/aif/temp/coolsnap/ ).

Roper sells an external fan that attaches to the camera with a
hose. Before placing the order for it (it has a 20% restocking fee if we
return it), I wanted to canvas other cooled CCD users to find out how
you've solved the vibrations due to air cooling problem and whether the
external fan unit solves the vibration problem.

Thanks.
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**



From MicroscopyL-request-at-ns.microscopy.com Wed May 25 13:57:09 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 25 May 2005 14:55:45 -0400
Subject: [Microscopy] Re: pacemakers and EM labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It is imperitive that you ask the people who make the pacemaker! What
are you going to do if one or more people on this list tell you that it
is ok when in fact it is not? I can hear the attorney for the family of
the dead student asking you, during the wrongful death suit, why you
took the advice of microscopists instead of contacting the manufacturer
of the pacemaker?

Geoff

Allan-Wojtas, Paula wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From MicroscopyL-request-at-ns.microscopy.com Wed May 25 14:00:28 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 25 May 2005 12:14:11 -0700
Subject: [Microscopy] Re: prions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Michael, contact me off-list and we will solve your problem. I need more
information (what camera, how it is installed, etc.).

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane
Duluth, GA 30096
Tel. (770)232-7785
Fax (770)232-1791
Mobile (678)467-0012
www.sia-cam.com

----- Original Message -----
} From: "Michael Cammer" {cammer-at-aecom.yu.edu}
To: {Microscopy-at-microscopy.com}
Sent: Wednesday, May 25, 2005 2:47 PM

I guess, client should fix tissue in 1.5-2% GA, then it's
safe. Personally, I would not do any immuno-work on it unless it's heavily
fixed. The main precaution is fixation - as soon it has been fixed - it's
safe. Sergey


At 11:07 AM 5/25/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Wed May 25 14:34:03 2005



From: Foran, David A :      DFORAN-at-ORA.FDA.GOV
Date: Wed, 25 May 2005 15:52:18 -0400
Subject: [Microscopy] Re: prions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ralph,

I was told that fixation does not effect the pathogenicity of prions. I may
stand corrected but never the less, this is something that you should
thoroughly investigated before handling and disposing of waste material.

Damian Neuberger



__________________________________________________________

Check the CDC site.

http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm

-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Wednesday, May 25, 2005 2:14 PM
To: Microscopy-at-microscopy.com

I guess, client should fix tissue in 1.5-2% GA, then it's
safe. Personally, I would not do any immuno-work on it unless it's heavily
fixed. The main precaution is fixation - as soon it has been fixed - it's
safe. Sergey


At 11:07 AM 5/25/2005, you wrote:


} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu




From MicroscopyL-request-at-ns.microscopy.com Wed May 25 14:54:42 2005



From: Bill Mollon :      bmollon-at-pacbell.net
Date: Wed, 25 May 2005 14:08:30 -0700 (PDT)
Subject: [Microscopy] Re: Re: vibrations in air cooled CCD cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ralph,

Anyone working with these pathogens would probably be wise to check with the
biosafety officer at their institution. In the end, you will need their
support.

--John | jchandler-at-ial-fa.com | 970.217.1321

----- Original Message -----
} From: "Damian Neuberger" {neuberger1234-at-comcast.net}
To: {Microscopy-at-microscopy.com}
Sent: Wednesday, May 25, 2005 1:33 PM

Before going to another camera vendor, please contact
Roper for the solution. There is one for this
problem.


--- Vitaly Feingold {vitalylazar-at-att.net} wrote:
}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Michael, contact me off-list and we will solve your
} problem. I need more
} information (what camera, how it is installed,
} etc.).
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane
} Duluth, GA 30096
} Tel. (770)232-7785
} Fax (770)232-1791
} Mobile (678)467-0012
} www.sia-cam.com
}
} ----- Original Message -----
} } From: "Michael Cammer" {cammer-at-aecom.yu.edu}
} To: {Microscopy-at-microscopy.com}
} Sent: Wednesday, May 25, 2005 2:47 PM
} Subject: [Microscopy] vibrations in air cooled CCD
} cameras
}
}
} }
} }
} }
}
------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
-------------------------------------------------------------------------------
} }
} } About a year ago we purchased a Roper CoolSnap HQ
} with fan in the camera
} } body. Problem is that we're getting a lot of
} vibrations. I installed a
} } switch in the wire to the fan. We may manually
} throw this switch to
} } disable the fan, but this is not a good solution
} because there is no
} } cooling airflow when the fan is off. (Examples
} are posted at
} } http://www.aecom.yu.edu/aif/temp/coolsnap/ ).
} }
} } Roper sells an external fan that attaches to the
} camera with a hose.
} } Before placing the order for it (it has a 20%
} restocking fee if we return
} } it), I wanted to canvas other cooled CCD users to
} find out how you've
} } solved the vibrations due to air cooling problem
} and whether the external
} } fan unit solves the vibration problem.
} }
} } Thanks.
} }
}
____________________________________________________________________________
} } Michael Cammer Analytical Imaging Facility
} Albert Einstein Coll. of
} } Med.
} } Jack & Pearl Resnick Campus 1300 Morris Park
} Ave. Bronx, NY
} } 10461
} } (718) 430-2890 Fax: 430-8996 URL:
} } http://www.aecom.yu.edu/aif/
} } **This electronic transmission contains
} information that is
} } privileged.**
} }
} }
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Wed May 25 16:25:59 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Wed, 25 May 2005 16:25:12 -0500
Subject: [Microscopy] Re: prions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ralph

as david pointed out by directing everyone to the CDC website - prions
are not pleasant things. they're extremely resistant to inactivation.
i remember one report of electrodes which had been inserted into the
brain of a CJD patient that were sterilized in paraformaldehyde gas,
followed by formaldehyde, followed by ethanol and then used again. the
second patient exposed to the electrodes developed CJD.

standard procedures for inactivation involve 1.0 to 4.0 Normal NaOH. it
destroys structure. i do not know whether there is any information
concerning the ability of OsO4 to inactivate the protein. even if it
did, there would be great risk in working with the tissue prior to the
OsO4 stage. you really should work at BSL 3, but might get away with
working at BSL2, and there should be some sense that the tissue is no
longer infectious before it leaves the containment lab for the EM
facility.

the most reasonable thing is that you make the investigator ensure the
tissue is inactivated, and that the safety office is comfortable with
the inactivation procedures before you let anything in the lab.

on the other hand, it will be 2-15 years before you know that you've
killed yourselves. if the university is lucky, they may even be able to
sell everyone on the idea that the real cause was you got a bad
hamburger made with some of our nasty canadian beef. not even the
bar-b-que will kill it, unless you cook like Ed Crankshaft. . . .

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Wed May 25 16:31:30 2005



From: K.N. Bozhilov :      bozhilov-at-ucr.edu
Date: Wed, 25 May 2005 14:29:46 -0700
Subject: [Microscopy] Hourly fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I need to find out the ranges of the currently prevailing hourly fees
that are charged in the US market for operation of SEMs and TEMs.

I am asking for input from academic, government as well as commercial
organizations. The rates should NOT include the charges for the
person operating the instrument and performing the imaging and/or
analysis. Also if possible, please specify whether it is for an
instrument with filed emission electron gun or not. While not
essential, information about the make, model, and year of
manufacturing of the instrument would be helpful.

Thank you,

Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel 951 827 2998
fax 951 827 2489


From MicroscopyL-request-at-ns.microscopy.com Wed May 25 16:33:41 2005



From: Karl Garsha :      garsha-at-itg.uiuc.edu
Date: Wed, 25 May 2005 16:33:00 -0500
Subject: [Microscopy] Re: viaWWW: Fluorescence microscopy Lamps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Kathleen,
There are advantages to using a fiber-coupled or liquid light guide
light source; the primary benefit is a more even illumination of the
field of view. The output coupling into the illumination system does
need to be done correctly, however, and there is a drop in illumination
intensity across the fiber. The reference below is pertinent:

Zvi, K. et. al. (1993). Design and construction of an optimal
illumination system for quantitative wide-field multi-dimensional
microscopy. Bioimaging 1; 71-81.

Regards,
Karl

by way of MicroscopyListserver wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Karl Garsha
Light Microscopy Specialist
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 North Mathews Avenue
Urbana, IL 61801
Office: B650J
Phone: 217.244.6292
Fax: 217.244.6219
Mobile: 217.390.1874
www.itg.uiuc.edu



From MicroscopyL-request-at-ns.microscopy.com Wed May 25 17:16:44 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Wed, 25 May 2005 18:46:46 -0500
Subject: [Microscopy] Re: prions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All:

Having started my professional career managing a EM lab working on CJD I
have actual experience working with this fun stuff. We found that PF &
Glut perfusions did not inactivate the mutant protein. Once the tissue was
post-fixed in OsO4 we could not serial pass the disease again. Once in
plastic it's for all intents and purposes inert. Waste materials were
autoclaved for a longer length of time than normal and discarded with
"normal" medical waste. In the early 80's when I was working with CJD it
was a BSL2 for the most part, now all procedures should be done under BSL3.
The Neuropathologist used to work with it under BSL1, removing the CJD
infected brains from the cadavers. They would always be violating
containment protocols in some was shape or form. They are still alive,
except one (CVA). The point I'm trying to make is don't over react, take
all of the prudent precautions of BSL3 you will be well protected.


Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com


----- Original Message -----
} From: "Ralph Common" {Ralph.Common-at-hc.msu.edu}
To: {Microscopy-at-microscopy.com}
Sent: Wednesday, May 25, 2005 2:07 PM

Hi, Ralph

You have not indicated exactly what you need to image, but re: prions, I'd recommend that your client contact Dr. Vitaly Vodyanoy (Vod-ja-noi) at Auburn University. (Email: see above). He has an optical approach which will probably solve the problem, with little or no preparation. He also knows the risks very well. Feel free to use my name.

Hope this is helpful

Barbara Foster
Microscopy/Microscopy Education
www.MicroscopyEducation.com

313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
F: 972-954-8018
^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&^&
Need a good general text on light microscopy? MME still has copies of Optimizing Light Microscopy available, with discounts for class-sized orders (10 or more). For details, call us at 972-943-8011 and ask for Ken Piel.
At 01:07 PM 5/25/2005, Ralph Common wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Wed May 25 19:30:06 2005



From: mcintyre-at-optics.rochester.edu (by way of MicroscopyListserver)
Date: Wed, 25 May 2005 19:29:24 -0500
Subject: [Microscopy] viaWWW: computer upgrade to LEO982

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mcintyre-at-optics.rochester.edu) from http://msa.microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, May 25, 2005 at 08:56:08
---------------------------------------------------------------------------

Email: mcintyre-at-optics.rochester.edu
Name: Brian McIntyre

Organization: Univ of Rochester

Title-Subject: [Microscopy] [Filtered] MListserver: computer upgrade to LEO982

Question: Hi -

Has anyone plugged in a new(er) single board computer to the LEO982 system? Mine has a 486DX2 and I'd really like to upgrade. I purchased a 233 Pentium SBC and got it to boot properly, but communication with the microscope hardware was tempermental (it didn't always respond to either keyboard or console commands).

Maybe somebody out there has already worked out the hardware configuration and would be willing to share it....?

Thanks!
Brian

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed May 25 19:30:49 2005



From: scitech200-at-yahoo.com (by way of MicroscopyListserver)
Date: Wed, 25 May 2005 19:30:06 -0500
Subject: [Microscopy] viaWWW: Amray 1645 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (scitech200-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, May 25, 2005 at 16:20:50
---------------------------------------------------------------------------

Email: scitech200-at-yahoo.com
Name: scitech-keith

Title-Subject: [Microscopy] [Filtered] Amray 1645 SEM

Question: As a hobbyist project (moving from light microscopy) I'm trying to get this SEM fully operational. I have vacuum and electronics expertise - but this is going to be a real challenge? I know I should really go to a professional service group, but was just wondering if anyone on this list is operating one of these and would be willing to assist with this project.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed May 25 19:31:14 2005



From: aweberg-at-siumed.edu (by way of MicroscopyListserver)
Date: Wed, 25 May 2005 19:30:30 -0500
Subject: [Microscopy] viaWWW: Sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (aweberg-at-siumed.edu) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, May 25, 2005 at 13:15:25
---------------------------------------------------------------------------

Email: aweberg-at-siumed.edu
Name: Aruna Weberg

Organization: SIU School of Medicine

Title-Subject: [Microscopy] [Filtered] Sputter coater

Question: Our facility is looking for a used/refurbished sputter coater with gold-palladium target, in reasonably good working condition to replace a 25 year old instrument. If your facility or organization has one available please contace me off line to discuss details.

Thank you

aruna

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed May 25 23:37:04 2005



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Thu, 26 May 2005 12:59:57 +0100 (BST)
Subject: [Microscopy] Laser-tweezer/confocal cell division Position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What camera vendor? All that needed is a fan and/or a heat sink. Or a
software command to stop fan during exposure. Not another
camera.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane
Duluth, GA 30096
Tel. (770)232-7785
Fax (770)232-1791
Mobile (678)467-0012
www.sia-cam.com

----- Original Message -----
} From: "Bill Mollon" {bmollon-at-pacbell.net}
To: "Vitaly Feingold" {vitalylazar-at-att.net} ; {Microscopy-at-microscopy.com} ;
"Michael Cammer" {cammer-at-aecom.yu.edu}
Sent: Wednesday, May 25, 2005 5:08 PM

I agree with Geoff that it would be useful to contact the pacemaker
manufacturer.

But if you look at the information and warnings supplied by the
manufacturers of NMRs (particularly the more recent super-conductors)
it is clear that they generate powerful fields that may interfere with
pacemakers. Some of the bigger Mass specs have very powerful magnetic
fields too. But the only warnings that I have ever seen from electron
microscope suppliers have been about the possibility of magnetic fields
interfering with them. You would suspect that the small pole-pieces in
the centre of the lens contains most of what must be a much weaker
field anyway. Of course all bets may be off if you're using a high
voltage em.

NMR manufacturers supply information about the shape and range of their
magnetic fields and usually warnings are only posted for about 10 to 20
feet from the magnet.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
UK
e-mail: TO:malcolm.haswell AND SEND TO: -at-sunderland.ac.uk
(NB REMOVE 'TO:' & ' AND SEND TO ')


----- Original Message -----
} From: Geoff McAuliffe {mcauliff-at-umdnj.edu}

Dear Listers,

A position for a postdoctoral researcher or a visiting
student is available at the Max-Planck Institute of
Molecular Cell Biology and Genetics in Dresden,
Germany.

The project includes construction of a confocal
microscope with integrated trapping and cutting beams,
followed by experiments on cell division in fission
yeast using this set-up.

Candidates with background in optics, confocal and
two-photon microscopy, and optical tweezers are
encouraged to apply. The position would be available
immediately. Please send your curriculum vitae, list
of publications, 1-3 selected papers, preferred
starting date, and names of three referees to Iva
Tolic-Norrelykke at tolic-at-mpi-cbg.de.
--------------------------------------------------------------

Apologies if you are on both lists, as I am, and get
this message twice - I ask for your gracious
understanding.
Regards,
Jeremy Sanderson



___________________________________________________________
How much free photo storage do you get? Store your holiday
snaps for FREE with Yahoo! Photos http://uk.photos.yahoo.com


From MicroscopyL-request-at-ns.microscopy.com Thu May 26 07:27:28 2005



From: hussar-at-antiqueswords.com (by way of MicroscopyListserver)
Date: Thu, 26 May 2005 07:26:46 -0500
Subject: [Microscopy] viaWWW: Nikon SMZ-10 Iris Diaphragm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (hussar-at-antiqueswords.com) from http://www.microscopy.com/MLFormMail.html on Wednesday, May 25, 2005 at 23:35:21
---------------------------------------------------------------------------

Email: hussar-at-antiqueswords.com
Name: Rob Miller

Organization: LionGate Arms & Armour

Title-Subject: [Microscopy] [Filtered] Nikon SMZ-10 Iris Diaphragm

Question: I am interested in obtaining an iris diaphragm (part #76270) for a Nikon SMZ-10. I am also looking for a 2x auxiliary lens.

Rob Miller
hussar-at-antiqueswords.com
602-740-8025

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu May 26 07:52:45 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Thu, 26 May 2005 05:52:03 -0700 (PDT)
Subject: [Microscopy] Re: Hourly fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If we send you this information, could you share the
results with us (without specifically naming the
labs)? I'm curious myself, since we rent out our SEM
services as well. I'm not sure how this would pan out
with board ethics... this could be a sensitive
subject. Then again, maybe not, since the engineering
journals report results of salary surveys.

Stu Smalinskas, P.E.
Metallurgist
SKF North American Technical Center
Plymouth, Michigan
(734) 414-6862
stu.smalinskas-at-skf.com

--- "K.N. Bozhilov" {bozhilov-at-ucr.edu} wrote:
}
} I need to find out the ranges of the currently
} prevailing hourly fees
} that are charged in the US market for operation of
} SEMs and TEMs.
}
} I am asking for input from academic, government as
} well as commercial
} organizations. The rates should NOT include the
} charges for the
} person operating the instrument and performing the
} imaging and/or
} analysis. Also if possible, please specify whether
} it is for an
} instrument with filed emission electron gun or not.
} While not
} essential, information about the make, model, and
} year of
} manufacturing of the instrument would be helpful.
}
} Thank you,
}
} Krassimir N. Bozhilov
} Central Facility for Advanced Microscopy and
} Microanalysis
} University of California
} Riverside, CA 92521
}
} tel 951 827 2998
} fax 951 827 2489
}
}



__________________________________
Do you Yahoo!?
Read only the mail you want - Yahoo! Mail SpamGuard.
http://promotions.yahoo.com/new_mail


From MicroscopyL-request-at-ns.microscopy.com Thu May 26 08:01:08 2005



From: Eric D. Johnston :      ericdj-at-seas.upenn.edu
Date: Thu, 26 May 2005 09:00:21 -0400 (EDT)
Subject: [Microscopy] Re: vibrations in air cooled CCD cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I had the same problem with the Coolsnap HQ. We had purchased our camera
a few years ago and had problems with vibration from the fan when we were
using micropipets. Our temporary solution was to put an extension (like
an empty magnifier) between the scope and the camera. I purchased the
quieter fan assembly from Roper recently, but I have not had a chance to
test it with the micropipets. Unfortunately, it sounds from the posting
like vibration is still a problem. For a camera that cost this much,
that's pretty sad.

Eric Johnston
University of Pennsylvania


On Thu, 26 May 2005, Vitaly Feingold wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} What camera vendor? All that needed is a fan and/or a heat sink. Or a
} software command to stop fan during exposure. Not another
} camera.
}
} Vitaly Feingold
} Scientific Instruments and Applications
} 2773 Heath Lane
} Duluth, GA 30096
} Tel. (770)232-7785
} Fax (770)232-1791
} Mobile (678)467-0012
} www.sia-cam.com
}
} ----- Original Message -----
} } From: "Bill Mollon" {bmollon-at-pacbell.net}
} To: "Vitaly Feingold" {vitalylazar-at-att.net} ; {Microscopy-at-microscopy.com} ;
} "Michael Cammer" {cammer-at-aecom.yu.edu}
} Sent: Wednesday, May 25, 2005 5:08 PM
} Subject: [Microscopy] Re: Re: vibrations in air cooled CCD cameras
}
}
} }
} }
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} } Before going to another camera vendor, please contact
} } Roper for the solution. There is one for this
} } problem.
} }
} }
} } --- Vitaly Feingold {vitalylazar-at-att.net} wrote:
} } }
} } }
} } }
} } ------------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The
} } } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } }


From MicroscopyL-request-at-ns.microscopy.com Thu May 26 08:03:35 2005



From: Alan Stone :      as-at-astonmet.com
Date: Thu, 26 May 2005 08:02:53 -0500
Subject: [Microscopy] Re: Re: Hourly fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would hope that all academic institutions who choose to rent out time on
their instruments to supplement their income also choose to pay their fair
share of income taxes on that revenue as well as contributing to their
local real estate taxes.

This is only fair to those of us contending with these tax burdens.

Alan Stone
ASTON




At 07:52 AM 5/26/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com



From MicroscopyL-request-at-ns.microscopy.com Thu May 26 08:23:14 2005



From: Karl Hagglund :      hagglundk1-at-nku.edu
Date: Thu, 26 May 2005 09:22:32 -0400
Subject: [Microscopy] Troubleshooting SEM Emission Current Zeiss DSM 960A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all who have responded so far to my query regarding
troubleshooting the DSM 960A (text below). I thought that I would
follow up with a bit more information after experimentation.

I did check the contact points the filament assembly plugs into, and
there was no obvious contamination. I cleaned these areas, and did not
pick up anything unusual or especially dirty.

The emission current reads 360-370 microAmps when there is no filament
(heating current reads zero), so this seems to represent a zero reading
for the emission. I was able to saturate the filament at higher
voltages (20 and 25kV) yesterday, and get an image (blurry but showing
real structures up to 100kX with maybe 50 nm resolution). At this
state, the current is reading only around 4 or 5 microamps higher than
the zero condition. Higher than this, and the current reading jumps to
470 or 480 with nothing obtainable in between (around 120 microAmps
above zero energy state) and starts the cycle of shorting until the
system reboots itself. This condition appears to be oversaturated, as I
quickly blew a filament (less than 1 hour at 470). This suggests that
the gauge has problems, so I'm checking out my schematics to see if I
can trace it to a single component.

I am trying to follow up on everyones leads, but if anyone else out
there has any ideas I would be thrilled to hear from them.

Thanks again.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu

Original Post:

I am working on the reinstallation of a Zeiss DSM 960A (1992). There are
two apparent problems.

1. The microscope is working at low voltages ( {10kV), but the emission
current seems to short out at higher voltages. It seems to modulate
between a saturated and unsaturated condition constantly, as if its
ramping up the voltage, shorts, and then ramps up again. Occasionally,
coincident with the shorting, the entire system restarts itself. I have
removed and cleaned the Wehnelt and anode assembly and there are no
obvious tungsten hairs or buildup of residues that might physically be
causing the short. Likewise, the vacuum is stable, and I am not seeing
any indications of a leak near the gun and have no vacuum errors.

I have been able to make it work at 10 and 20kV, but have to saturate
very carefully, and even then the system does not appear to be stable.

2. The emission current is reading much higher than expectation. In an
unsaturated condition, the gauge reads approximately 370 milliAmps (I
believe it should read zero). When the filament is saturated it is
reading 460-480 uA (the manual suggests 80).



Does anyone out there have one of these microscopes and/or has someone
seen these problems before on this model or a similar one? Thanks in
advance for all your help. The list always seems to come through for
me.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu




From MicroscopyL-request-at-ns.microscopy.com Thu May 26 10:50:49 2005



From: William A. Monroe :      monroe-at-emcenter.msstate.edu
Date: Thu, 26 May 2005 10:50:03 -0500
Subject: [Microscopy] Light Microscope Service (Third Party Contact Information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

Can anyone recommend a third party service company for a Zeiss
Axiovert 135. The scope is located in Mississippi.

Please contact me off line.


Best Wishes,
Bill Monroe
--
Bill Monroe
Electron Microscope Center
103 Clay Lyle Entomology Building
Mississippi State University
Mississippi State, MS 39762
(662)-325-3019 Work
(662)-323-5246 Home
(662)-325-0246 Fax


From MicroscopyL-request-at-ns.microscopy.com Thu May 26 11:31:07 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Thu, 26 May 2005 11:32:19 -0500
Subject: [Microscopy] Ethanol & antibody permeabilization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i know that fixation in acetone permeabilizes tissue pieces so
that one can do whole mount immuno staining of tissues measuring about 3 mm
x 3 mm x 3 mm. I can't use acetone for a particular experiment and want to
fix in ice cold ethanol. Does anybody know from experience if ethanol
fixation permeabilizes the tissue enough for antibody penetration into
intracellular epitopes? I don't want to use aldehyde fixes with triton
x-100 or saponin. Thanks, tom



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
3 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Thu May 26 11:51:27 2005



From: Microscopy Today :      microscopytoday-at-tampabay.rr.com
Date: Thu, 26 May 2005 12:50:42 -0400
Subject: [Microscopy] Articles for Microscopy Today Solicited

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

A great many of you have subscriptions to Microscopy Today magazine.

I get roughly 25% of the articles in MT via authors sending them to me
for consideration. The bulk of MT content comes from my inviting
articles from the M&M meeting abstracts or the programs of local
affiliate society meetings. I'm usually frantically making phone calls
trying to hustle articles a month before an issue goes to the printer.
I'm becoming too old for frantic.

I would like to obtain candidate article submissions to the point where
I have an issue's-worth of articles backlogged.

I need 12-14 articles per issue. I have 10 articles in hand for the July
issue and promises of 2 more. I could use a couple more for July and I'd
like to get articles for the September issues in ASAP so that we can
consider taking a little time in Hawaii for a bit of a vacation after
the M&M meeting. Contact me for deadlines.

Please consider this an invitation to submit articles for MT.

MT goes to nearly 15,000 readers--way more than most journals. The only
thing in common among MT readers is that they are microscopists. As a
result of this diversity, articles written for a niche audience would be
better off submitted to an appropriate specialist journal. I like to
call the content of MT "articles" instead of "papers." MT articles
should be somewhat tutorial in nature as MT is often read cover-to-cover
and should educate the reader in fields that they are not acquainted
with. The articles do not receive peer review for the most part,
however, I will obtain peer reviews as needed. Overtly commercial
articles that read like press releases are not acceptable, although we
are happy to publish articles that bring new instruments/equipment to
the attention of the reader written in a scientific style. As editor,
I've been trying to maintain a balance between the various types of
microscopy and the various disciplines.

The length target for MT articles is 2 to 4 MT pages. An MT page with no
figures is about 1,100 words. We love lots of figures and graphics
(color is appreciated); their inclusion will reduce the number of words
needed per page. An M&M two-page abstract expanded by 25 to 50% with a
few extra pictures is a good fit. Shorter contributions are welcomed in
our Microscopy-101 section.

MT articles are a useful pedagogical exercise for senior graduate
students that can teach the methods of writing about highly technical
work in an easily understood tutorial style.

Contact me to obtain a copy of our Instructions to Authors document.
Looking forward to hearing from you!

Ron Anderson, Editor
Microscopy Today





From MicroscopyL-request-at-ns.microscopy.com Thu May 26 15:44:28 2005



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Thu, 26 May 2005 16:43:48 -0400
Subject: [Microscopy] Re: vibrations in air cooled CCD cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you for your input on and off-list regarding the vibrations problem.

It looks as though the most likely solution is going to be an external fan
unit attached by a flexible duct to the back of the camera.

We will try this first and post a brief message when we have the system
running regarding how the problem was solved.

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**



From MicroscopyL-request-at-ns.microscopy.com Thu May 26 18:10:27 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Thu, 26 May 2005 18:09:44 -0500
Subject: [Microscopy] imaging shearing in hydrated samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a user who would like to be able to image the effect of shearing on a
hydrated clay slurry. We would like to be able to subject the sample to
shearing stress and then immediately freeze it. We would then image the
sample using cryo-FESEM.

Our problem is how to go about doing this. One thought was to place a small
amount of the slurry between two surfaces such as sample holders used in
high pressure freezing and quickly rotate and plunge. Another thought was
possibly using a vitrobot to assist in doing this.

We would appreciate any suggestions from those of you who might have
experience in this area. Information on references that describe shearing
in similar systems using SEM imaging would be appreciated.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy








From MicroscopyL-request-at-ns.microscopy.com Thu May 26 18:40:40 2005



From: Ralph Common :      Ralph.Common-at-hc.msu.edu
Date: Thu, 26 May 2005 19:39:45 -0400
Subject: [Microscopy] prions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to thank everybody who replied privately, or to the group, to my posting concerning prions. The comments were very informative and helpful, as is usually the case with this group. The information helped me be comfortable with my decision, which was to have the client process the tissue within a BSL2 facility, and work with the tissue only after it was in resin.

Ralph Common
Michigan State University
Division of Human Pathology




From MicroscopyL-request-at-ns.microscopy.com Thu May 26 21:05:02 2005



From: Pat Kelly :      probe-at-geotrack.com.au
Date: Fri, 27 May 2005 12:04:19 +1000
Subject: [Microscopy] EDAX ECON-2 detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have just acquired an EDAX ECON-2 detector for our JSM35 but with no
manuals. Does anyone out there have an operating unit or possibly operating
instructions. I have no experience with windowless detectors and some feed
back would be great before I cool it down and crank up the voltage.
Thanks
Pat

Patrick R Kelly Operations Manager
Geotrack International Pty Ltd ABN16 006 821 209
37 Melville Road, Brunswick West, Victoria 3055 Australia
Telephone: +613 93801077 Facsimile: +613 93801477
http://www.geotrack.com.au



From MicroscopyL-request-at-ns.microscopy.com Thu May 26 22:58:13 2005



From: Pat Kelly :      probe-at-geotrack.com.au
Date: Fri, 27 May 2005 12:04:19 +1000
Subject: [Microscopy] EDAX ECON-2 detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

We have just acquired an EDAX ECON-2 detector for our JSM35 but with no
manuals. Does anyone out there have an operating unit or possibly operating
instructions. I have no experience with windowless detectors and some feed
back would be great before I cool it down and crank up the voltage.
Thanks
Pat

Patrick R Kelly Operations Manager
Geotrack International Pty Ltd ABN16 006 821 209
37 Melville Road, Brunswick West, Victoria 3055 Australia
Telephone: +613 93801077 Facsimile: +613 93801477
http://www.geotrack.com.au




From MicroscopyL-request-at-ns.microscopy.com Fri May 27 08:07:50 2005



From: Joseph P Neilly :      joe.p.neilly-at-abbott.com
Date: Fri, 27 May 2005 08:06:43 -0500
Subject: [Microscopy] Looking for a source of thin, strong wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I need a wire or metal probe to push through a hypodermic needle. It has
to be 0.2 mm in diameter and strong enough to travel through a 20 mm
needle. The wires from our metal evaporator are to soft. Copper is to
soft too. Tungsten probes are strong enough, but the ones we have are to
fat. Any suggestions?


Joe Neilly, Research Investigator
Abbott Laboratories
R4R9, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027
E-mail: joe.neilly-at-abbott.com



From MicroscopyL-request-at-ns.microscopy.com Fri May 27 08:28:42 2005



From: White, Woody N. :      NWWhite-at-bwxt.com
Date: Fri, 27 May 2005 09:27:46 -0400
Subject: [Microscopy] Looking for a source of thin, strong wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Joe,

Although rather expensive, www.goodfellow.com can fill your need. For
example, they have 0.15mm drawn iridium wire. It is hard, stiff, noble
- and pricy.

I did not look, but perhaps they have a suitable wire in a cheaper
material.

Regards,
Woody


-----Original Message-----
} From: Joseph P Neilly [mailto:joe.p.neilly-at-abbott.com]
Sent: Friday, May 27, 2005 9:07 AM
To: microscopy-at-microscopy.com

I need a wire or metal probe to push through a hypodermic needle. It
has
to be 0.2 mm in diameter and strong enough to travel through a 20 mm
needle. The wires from our metal evaporator are to soft. Copper is to
soft too. Tungsten probes are strong enough, but the ones we have are
to
fat. Any suggestions?


Joe Neilly, Research Investigator
Abbott Laboratories
R4R9, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027
E-mail: joe.neilly-at-abbott.com





From MicroscopyL-request-at-ns.microscopy.com Fri May 27 08:32:41 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 27 May 2005 08:31:58 -0500
Subject: [Microscopy] Looking for a source of thin, strong wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I can't remember specifics as to wire type, etc., but I did some
graduate research years ago on electromyography of forearm musculature.
I pushed double strands of fine electrical wire through 25-gauge
needles, burned off the first 2-3 mm of insulation, bent the ends back,
sterilized the package, and inserted the needle into the muscle of
interest. (Yes, it hurt...) The wire was strong enough to thread into
the needle, puncture and remain in muscle while the needle was removed,
and remain inserted and hooked up to an FM transmitter through a couple
hours of some very odd exercises.

I will try to dig up that paper, but in the meantime do a search in the
bio abstracts on electromyography of muscle and I bet you'll find lots
of references with specifics about wires, etc.

Hope it helps.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
} From: Joseph P Neilly [mailto:joe.p.neilly-at-abbott.com]
Sent: Friday, May 27, 2005 8:07 AM
To: microscopy-at-microscopy.com

I need a wire or metal probe to push through a hypodermic needle. It
has to be 0.2 mm in diameter and strong enough to travel through a 20 mm
needle. The wires from our metal evaporator are to soft. Copper is to
soft too. Tungsten probes are strong enough, but the ones we have are
to fat. Any suggestions?


Joe Neilly, Research Investigator
Abbott Laboratories
R4R9, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027
E-mail: joe.neilly-at-abbott.com





From MicroscopyL-request-at-ns.microscopy.com Fri May 27 08:54:08 2005



From: frank.karl-at-degussa.com
Date: Fri, 27 May 2005 09:53:14 -0400
Subject: [Microscopy] Re: Looking for a source of thin, strong wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Joe,
Try McMaster-Carr in Cleveland Ohio (330-995-5500)

My conversion from mm to inch (if i did it right) is 0.0078 inch.

McMasters sell Chromel C wire thats 0.0063 inch (1015 ft coil) for $23
A tinned copper 0.005 (3200 ft coil) $9
Nitinol wire 0.006 (30ft coil) $30
Stainless steel 0.007 (3723 ft coil) for $33.

Good luck

Oh, by the way McMaster-Carr doesn't know me from Adam and I have no
connection to them...



Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.



"Joseph P Neilly"
{joe.p.neilly-at-abb To: microscopy-at-microscopy.com
ott.com} cc:
Subject: [Microscopy] Looking for a source of thin, strong wire
05/27/2005 09:06
AM







------------------------------------------------------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I need a wire or metal probe to push through a hypodermic needle. It has
to be 0.2 mm in diameter and strong enough to travel through a 20 mm
needle. The wires from our metal evaporator are to soft. Copper is to
soft too. Tungsten probes are strong enough, but the ones we have are to
fat. Any suggestions?


Joe Neilly, Research Investigator
Abbott Laboratories
R4R9, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202

Voice: 847-938-5024
Fax: 847-938-5027
E-mail: joe.neilly-at-abbott.com







From MicroscopyL-request-at-ns.microscopy.com Fri May 27 09:14:39 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Fri, 27 May 2005 07:13:57 -0700 (PDT)
Subject: [Microscopy] Re: Looking for a source of thin, strong wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joe:

Music wire comes to mind, if steel is okay for your
application. Music wire is a generic term for steel
wire that is severly cold drawn, making it strong and
stiff. I imagine this wire should be available at any
music store.

I also remember using a stainless steel version (Grade
302, 304, or 316) of this type of wire, though I can't
remember from where we bought it, or if it's available
in the thin (0.008") diameter you need.

Stu Smalinskas, P.E.
Metallurgist
SKF
Plymouth, Michigan


--- Joseph P Neilly {joe.p.neilly-at-abbott.com} wrote:

} I need a wire or metal probe to push through a
} hypodermic needle. It has
} to be 0.2 mm in diameter and strong enough to travel
} through a 20 mm
} needle. The wires from our metal evaporator are to
} soft. Copper is to
} soft too. Tungsten probes are strong enough, but
} the ones we have are to
} fat. Any suggestions?
}
}
} Joe Neilly, Research Investigator
} Abbott Laboratories
} R4R9, AP31
} 200 Abbott Park Rd.
} Abbott Park, IL 60064-6202
}
} Voice: 847-938-5024
} Fax: 847-938-5027
} E-mail: joe.neilly-at-abbott.com
}


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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From MicroscopyL-request-at-ns.microscopy.com Fri May 27 09:20:23 2005



From: Ivan Petrov :      petrov-at-mrl.uiuc.edu
Date: Fri, 27 May 2005 09:19:42 -0500
Subject: [Microscopy] a reminder for MMMS05

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

This is a kind reminder to attend the 2005 Midwest Microscopy and
Microanalysis Society on Dynamics of Materials Revealed by Electron
Microscopy in Urbana June 9-10.

http://cmm.mrl.uiuc.edu/MMMS05/Program.htm

regards

Ivan Petrov



From MicroscopyL-request-at-ns.microscopy.com Fri May 27 10:32:32 2005



From: David Henriks :      henriks-at-southbaytech.com
Date: Fri, 27 May 2005 08:31:47 -0700
Subject: [Microscopy] Re: Re: Looking for a source of thin, strong wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joe:

We use fine wires for our wire saw and have plain stainless steel wires
in .005", .010" and .015". I may have some .003" as well. It sounds
like you need the .010" diameter. I'd be happy to send you a length of
the wire. Let me know how much you need and which diameter you'd like.
We also make these same cores into diamond wire as well. I can provide
that if it would help, but I would have to charge you for the diamond
wire. I hope that helps.

DISCLAIMER: South Bay Technology produces diamond wire as well as
precision wire saws as described above and, therefore, has a vested
interest in promoting their use.

Best regards-

David

--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.



frank.karl-at-degussa.com wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.






From MicroscopyL-request-at-ns.microscopy.com Fri May 27 10:44:16 2005



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 27 May 2005 17:42:34 +0200
Subject: [Microscopy] Re: Looking for a source of thin, strong wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


----- Original Message -----
} From: "Mary Mager" {mager-at-interchange.ubc.ca}
To: "Joseph P Neilly" {joe.p.neilly-at-abbott.com}
Cc: "Microscopy" {microscopy-at-MSA.microscopy.com}
Sent: Friday, May 27, 2005 8:40 AM


Go to your local music shop, and by a guitar E string (first). Carbon
steel, 0.18 or 0.2 mm diameter. Banjo, or mandoline first string is the
same. You may have a wide choice... in price !

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Fri, 27 May 2005, Joseph P Neilly wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} I need a wire or metal probe to push through a hypodermic needle. It has
} to be 0.2 mm in diameter and strong enough to travel through a 20 mm
} needle. The wires from our metal evaporator are to soft. Copper is to
} soft too. Tungsten probes are strong enough, but the ones we have are to
} fat. Any suggestions?
}
}
} Joe Neilly, Research Investigator
} Abbott Laboratories
} R4R9, AP31
} 200 Abbott Park Rd.
} Abbott Park, IL 60064-6202
}
} Voice: 847-938-5024
} Fax: 847-938-5027
} E-mail: joe.neilly-at-abbott.com
}
}




From MicroscopyL-request-at-ns.microscopy.com Fri May 27 12:10:18 2005



From: Skage Hem :      SHem-at-laurentian.ca
Date: Fri, 27 May 2005 13:09:01 -0400
Subject: [Microscopy] Documentation for a Cambridge S-120

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello there,
We have an (almost) functioning Cambridge S-120 SEM. This instrument has been
the responsibility of several persons, so the available documentation is sparse.
I'm looking for an operating manual, installation manual and most important
electrical schematics for the instrument. Do anyone have this or know of a source ?
Any advice is greatly appreciated.
Cheers,

Skage Hem



_______________________________________________________________

Skage Hem, Ph.D.
Research Scientist
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html




From MicroscopyL-request-at-ns.microscopy.com Fri May 27 15:20:04 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 27 May 2005 16:16:20 -0400
Subject: [Microscopy] Re: Ethanol & antibody permeabilization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tom:

There was a paper in J. Histochem. Cytochem some time ago about
using graded ethanols, buffered with phosphate, to permeabilize tissue
for immunostaining. Digging through the files now ...........

Eldred, W.D. et al JHC 31:285-292, 1983
Versaux-Botter and Nguyen-Legros JHC 34:743-747, 1986
Llewellyn-Smith and Minson JHC 40:1741-1749, 1992

I tried the graded, buffeded ethanol method some time ago on
sections destined for pre-embedding immuno for TEM, worked well and
ultrastructure was tolerable.

Geoff


Tom Phillips wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} i know that fixation in acetone permeabilizes tissue pieces so
} that one can do whole mount immuno staining of tissues measuring about
} 3 mm x 3 mm x 3 mm. I can't use acetone for a particular experiment
} and want to fix in ice cold ethanol. Does anybody know from experience
} if ethanol fixation permeabilizes the tissue enough for antibody
} penetration into intracellular epitopes? I don't want to use aldehyde
} fixes with triton x-100 or saponin. Thanks, tom
}
}
}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
}
}


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From MicroscopyL-request-at-ns.microscopy.com Fri May 27 16:23:15 2005



From: Gordon Couger :      gcc-at-couger.com
Date: Fri, 27 May 2005 16:22:32 -0500
Subject: [Microscopy] Re: Looking for a source of thin, strong wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joe,

If you could chemically mill 27 pound stainless steel wire
fishing leader that is .28 mm in diameter. Another approach is
to stretch it enough to reduce the diameter either by reaching
cold by heating it and a stretching it or a combination of both
with the final stage be the cold stretching to work harden it.
You will probably be able to anneal the wire stretch it a bit,
anneal it and stretch it and repeat until you get the desired
diameter. Clamping the wire to the jaws of large vice and
opening the vise some more should supply enough force to stretch
the wire.

The conventional way to do this is to draw it through a die but
the cost of the die is probably prohibitive for your job.

The wire is sure cheap enough at a half cent a foot.
http://www.anglerscenter.com/terminal_rigging_wire.htm
Tackle Town (361) 729-1841 3010 Highway 35 N Rockport, TX will
ship it if you order several. It is handy stuff to have around
the lab.

You might try taking some copper that is a little too large and
stretching it until it breaks. I will become smaller as you
stretch it and work harden and become stiffer in the process.

Best Regards
Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org

Joseph P Neilly wrote:
}
} I need a wire or metal probe to push through a hypodermic
needle. It has
} to be 0.2 mm in diameter and strong enough to travel through
a 20 mm
} needle. The wires from our metal evaporator are to soft.
Copper is to
} soft too. Tungsten probes are strong enough, but the ones we
have are to
} fat. Any suggestions?
}
}
} Joe Neilly, Research Investigator
} Abbott Laboratories
} R4R9, AP31
} 200 Abbott Park Rd.
} Abbott Park, IL 60064-6202
}
} Voice: 847-938-5024
} Fax: 847-938-5027
} E-mail: joe.neilly-at-abbott.com
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri May 27 20:28:02 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 27 May 2005 18:27:14 -0700
Subject: [Microscopy] Re: Re: Looking for a source of thin, strong

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I agree with this assessment. Get a light gauge E string
for guitar or 12-string and this should work. These are
sold as sets or separately. One unit of wire/string is
typically about $2USD, depending on quality and brand.

I use D'Angellico and Martin strings for my guitars. Of course,
I have no financial interest in Martin, Guild, Taylor,
Rickenbacker or other instrument makers, or any strings that
they may supply.

gary g.


At 08:42 AM 5/27/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Sun May 29 21:16:17 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 29 May 2005 14:49:53 -0700
Subject: [Microscopy] specimen heating up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers:

I've encountered a once only situation that I cannot
explain. Perhaps someone has some ideas about what
is going on.

I put a specimen in the SEM for examination and when
I pulled it out via the load lock, the specimen and
holder were about 10 degrees hotter than ambient.
My experience has been that whatever they go in at,
they come out the same. This was simple SE imaging
at 10KV with 30u High Current in Zeiss Supra 55VP
in high vacuum mode. Specimen current was around 145pA.

The specimen was a copper tube that had been plated
with silver on the outside and inside. The piece was
one half of the tube and about .5" long, attached to
a pin stub using colloidal silver.

EDS showed presence of K, O, Ag, Cu, and C. There were
distinct areas of organic material that I take to be
cyanide from the plating process. But how could SEM
analysis of a specimen cause it to heat?

Any ideas?

gary g.



From MicroscopyL-request-at-ns.microscopy.com Mon May 30 01:12:38 2005



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Mon, 30 May 2005 16:11:20 +1000
Subject: [Microscopy] Leitz Durimet

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been asked to see if we can bring our old Microhardness Tester Leitz Durimet
back to life.
Unfortunately someone has damaged both objectives beyond any repair.

I try to find a source that can offer me a suitable high power measuring objective:
Type A 0.70 C HM 6.3 40x
and a low power scanning objective
Type A 0.18 C HM25 10x

Someone might even have an old used Durimet unit in their cupboards. We are willing to make a deal.

Cheers



Hans Brinkies
Professional Officer
Electron Microscopy & Metallography
Swinburne, University of Technology
Faculty of Engineering and Industrial Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Mobile: 0417 156 267
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au


Swinburne University of Technology
CRICOS Provider Code: 00111D

NOTICE
This e-mail and any attachments are confidential and intended only for the use of the addressee. They may contain information that is privileged or protected by copyright. If you are not the intended recipient, any dissemination, distribution, printing, copying or use is strictly prohibited. The University does not warrant that this e-mail and any attachments are secure and there is also a risk that it may be corrupted in transmission. It is your responsibility to check any attachments for viruses or defects before opening them. If you have received this transmission in error, please contact us on +61 3 9214 8000 and delete it immediately from your system. We do not accept liability in connection with computer virus, data corruption, delay, interruption, unauthorised access or unauthorised amendment.





From MicroscopyL-request-at-ns.microscopy.com Mon May 30 20:56:36 2005



From: armandm-at-uidaho.edu (by way of MicroscopyListserver)
Date: Mon, 30 May 2005 20:55:54 -0500
Subject: [Microscopy] viaWWW:Manual for Sorvall JB 4 microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (armandm-at-uidaho.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, May 30, 2005 at 20:45:54
---------------------------------------------------------------------------

Email: armandm-at-uidaho.edu
Name: Armando McDonald

Organization: University of Idaho

Title-Subject: [Microscopy] [Filtered] Sorvall JB 4 microtome

Question: I have just inherited a Soravll JB4 microtome and glass knife cutter for my lab, however, it came without an operating manual.

Has anyone got a manual for such a unit or a copy of one?

Any help is much appreciated.

Cheers,
Armando



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue May 31 11:57:33 2005



From: K.N. Bozhilov :      bozhilov-at-ucr.edu
Date: Tue, 31 May 2005 09:56:19 -0700
Subject: [Microscopy] Re:Hourly rates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

First, I would like to thank everybody who replied to my inquiry
concerning TEM and SEM hourly fees.

I received 12 replies although I hoped to get many more responses.
Still I was able to get some information from the replies as well as
from information posted on various web cites.

Here is a summary of the data I have collected. It should be regarded
as information about the current hourly charges but not the actual
costs of operation of TEMs and SEMs since many organizations have
very specific approaches in subsidizing or funding EM operations.
Also the data collected is summary from only about 40 colleges,
universities and government agencies and it is not clear to me what
is the statistical significance of the obtained data. For the
commercial rates the data comes only from four sources and it clearly
is not statistically reliable.

Krassimir Bozhilov

____________________________________________________________

Rates for hourly operation of TEM and SEM not including operator costs

Universities and Government agencies - internal rates
TEM - Mean $35
Median $36
Max. $60
Min. $11

TEM-FEG Mean $71
Median $75
Max. $90
Min. $25

SEM - Mean $33
Median $32
Max. $55
Min. $11

SEM-FEG - Mean $48
Median $50
Max. $80
Min. $25

Universities and Government agencies - rates for other government or
educational organizations
TEM - Mean $66
Median $50
Max. $185
Min. $30

TEM-FEG Mean $119
Median $90
Max. $250
Min. $66

SEM - Mean $73
Median $55
Max. $240
Min. $25

SEM-FEG - Mean $84
Median $80
Max. $135
Min. $40


Universities and Government agencies - rates for commercial users

TEM - Mean $111
Median $96
Max. $250
Min. $30

TEM-FEG Mean $164
Median $158
Max. $250
Min. $90

SEM - Mean $121
Median $100
Max. $250
Min. $35

SEM-FEG - Mean $122
Median $112
Max. $200
Min. $40



Commercial labs (most rates include operator costs)

TEM - Mean $322
Median $335
Max. $370
Min. $250

TEM-FEG Mean $375
Median $375
Max. $400
Min. $375

SEM - Mean $216
Median $223
Max. $250
Min. $180

SEM-FEG - Mean $262
Median $300
Max. $330
Min. $170


From MicroscopyL-request-at-ns.microscopy.com Tue May 31 18:29:10 2005



From: rtemkin-at-mtsinai.on.ca (by way of MicroscopyListserver)
Date: Tue, 31 May 2005 18:28:27 -0500
Subject: [Microscopy] viaWWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rtemkin-at-mtsinai.on.ca) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Tuesday, May 31, 2005 at 12:25:57
---------------------------------------------------------------------------

Email: rtemkin-at-mtsinai.on.ca
Name: Robert Temkin

Organization: Hospital for Sick Children Research Institute, Toronto, Canada

Title-Subject: [Microscopy] [Filtered] osmium fixation of cultured cells

Question: I have recently been having problems getting good staining of the membranes of cultured cells with osmium. The cells were fixed with 2% glutaraldehyde and post fixed in 1% osmium tetroxide. I have tried 0.1M phosphate buffer, pH 7.4 and 0.1M cacodylate buffer pH 7.3. When using these the membranes were not visible at all. I also tried osmium with potassium ferrocyanide which worked well with phosphate buffer but left a black precipitate. With cacodylate buffer and potassium ferrocyanide the membrane definition was a little better but still not adequate. If anyone has any suggestions as to how to improve the membrane staining, they would be greatly appreciated. Thank you.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue May 31 18:37:03 2005



From: tkelly-at-imago.com (by way of MicroscopyListserver)
Date: Tue, 31 May 2005 18:36:20 -0500
Subject: [Microscopy] viaWWW: event honoring the 50th Anniversary of the Atomic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tkelly-at-imago.com) from http://microscopy.com/MLFormMail.html on Tuesday, May 31, 2005 at 18:34:55
---------------------------------------------------------------------------

Email: tkelly-at-imago.com
Name: Tom Kelly

Organization: Imago

Title-Subject: [Microscopy] [Filtered] event honoring the 50th Anniversary of the Atomic Resolution Imaging

Question: Dear Microscopist,

This note is being sent to you to announce a special event honoring the 50th Anniversary of the Atomic Resolution Imaging, dedicated to the memory of Erwin W. Muller. The conference will take place on June 15-17 at the Nittany Lion Inn on the Pennsylvania State University campus, State College, Pennsylvania. Please see the following website for details about this exciting historic celebration, such as speakers, events and location.

http://app.outreach.psu.edu/atomicresolution/default.asp?WhichPage=default

There are a small number of travel scholarships supported by the US National Science Foundation available for young faculty and students from other universities. If you are interested, please contact me directly at the information below. Thank you for your time and we hope you can attend!

Kind Regards,

Tom Kelly
Thomas F. Kelly, Ph.D.
Founder, Chairman and CTO
Imago Scientific Instruments Corporation
6300 Enterprise Lane
tkelly-at-imago.com
Madison, WI 53719-1193
{http://www.imago.com/} www.imago.com
Phone: +1.608.274.6880 x211 or +1.877.Go.Imago x211
Fax: +1.608.442.0622

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue May 31 18:58:54 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Tue, 31 May 2005 19:58:09 -0400
Subject: [Microscopy] Re: viaWWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Getting good contrast when embedding a cell monolayer
is often a problem for us too. I have always suspected extraction,
during dehydration. Are you using "en bloc" staining with
uranyl acetate? It might be considered optional by some, but
I find it is necessary for good membrane contrast when working
with monolayers.
Good luck

Marc

On Tuesday, May 31, 2005, at 07:28 PM, by way of MicroscopyListserver
wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (rtemkin-at-mtsinai.on.ca) from
} http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on
} Tuesday, May 31, 2005 at 12:25:57
} -----------------------------------------------------------------------
} ----
}
} Email: rtemkin-at-mtsinai.on.ca
} Name: Robert Temkin
}
} Organization: Hospital for Sick Children Research Institute, Toronto,
} Canada
}
} Title-Subject: [Microscopy] [Filtered] osmium fixation of cultured
} cells
}
} Question: I have recently been having problems getting good staining
} of the membranes of cultured cells with osmium. The cells were fixed
} with 2% glutaraldehyde and post fixed in 1% osmium tetroxide. I have
} tried 0.1M phosphate buffer, pH 7.4 and 0.1M cacodylate buffer pH 7.3.
} When using these the membranes were not visible at all. I also tried
} osmium with potassium ferrocyanide which worked well with phosphate
} buffer but left a black precipitate. With cacodylate buffer and
} potassium ferrocyanide the membrane definition was a little better but
} still not adequate. If anyone has any suggestions as to how to improve
} the membrane staining, they would be greatly appreciated. Thank you.
}
} -----------------------------------------------------------------------
} ----
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Tue May 31 19:38:24 2005



From: Webster, Paul :      PWebster-at-hei.org
Date: Tue, 31 May 2005 17:35:04 -0700
Subject: [Microscopy] Re: viaWWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Robert,

You don't mention whether you scrape the cells before processing or fix and
process them in situ. This may affect the contrast because a pellet can be
less easily penetrated by your chemicals.

Neither do you say which resin you are using. Spurr resin will give you less
contrast than Epon (or Epon substitute).

There is a method used by Lou Tilney that supposedly produces good contrast
when used on cells in culture dishes (see Tilney, L.G. And D.A. Portnoy 1989
Actin filaments and the growth, movement, and spread of the intracellular
bacterial parasite, Listeria monocytogenes. J Cell Biol. 1989
Oct;109:1597-608 for details). I think he mixes glutaraldehyde with the
osmium tetroxide in a buffer at pH 6.2, but maybe Pat Connelley could supply
more details on this. However, the cells have to be processed in the dish.

Other, less drastic things you can try are to use uranyl acetate as an en
bloc stain, as suggested by Marc. It works really well when used at 0.5% to
1% in 50mM maleate buffer. Alternatively, make up a saturated solution of
uranyl acetate in 70% methanol and leave your cells in this overnight.

If you Like the contrast produced by the reduced osmium, just work at
removing the black precipitate. It is caused by a reaction between the
phosphate buffer, glutaraldehyde and osmium. Wash the aldehyde and/or the
phosphate buffer away and all will be well.

Of course, you can also manipulate contrast with the electron microscope. A
smaller objective aperture will give you more specimen contrast, but less
resolution. However, if you are working for good specimen contrast, there is
a high probability that you have low specimen resolution anyway - you are
washing away and aggregating the intracellular components.

Good luck.

Paul Webster.



Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org








On 5/31/05 4:28 PM, "by way of MicroscopyListserver" {rtemkin-at-mtsinai.on.ca}
wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted
} by (rtemkin-at-mtsinai.on.ca) from
} http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Tuesday, May
} 31, 2005 at 12:25:57
} ---------------------------------------------------------------------------
}
} Email: rtemkin-at-mtsinai.on.ca
} Name: Robert Temkin
}
} Organization: Hospital for Sick Children Research Institute, Toronto, Canada
}
} Title-Subject: [Microscopy] [Filtered] osmium fixation of cultured cells
}
} Question: I have recently been having problems getting good staining of the
} membranes of cultured cells with osmium. The cells were fixed with 2%
} glutaraldehyde and post fixed in 1% osmium tetroxide. I have tried 0.1M
} phosphate buffer, pH 7.4 and 0.1M cacodylate buffer pH 7.3. When using these
} the membranes were not visible at all. I also tried osmium with potassium
} ferrocyanide which worked well with phosphate buffer but left a black
} precipitate. With cacodylate buffer and potassium ferrocyanide the membrane
} definition was a little better but still not adequate. If anyone has any
} suggestions as to how to improve the membrane staining, they would be greatly
} appreciated. Thank you.
}
} ---------------------------------------------------------------------------
}



From MicroscopyL-request-at-ns.microscopy.com Tue May 31 22:56:20 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Tue, 31 May 2005 22:59:44 -0500
Subject: [Microscopy] Re: viaWWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

robert

i had problems with my monolayers and suspensions for a while. i
reviewed my procedures and found two things.

1. i had changed from acetone to ethanol dehydration. i'm not sure,
but i believe i first saw this in Pease's procedures book - 2nd
edition. it referred to the ongoing problem with loss of membranes when
ethanol extraction is used. subsequent studies had shown that osmium
did not adequately stabilize membranes against extraction. when i went
back to acetone i solved all the problems. unfortunately you cannot use
acetone all the time, however.

2. en bloc staining with uranyl acetate, as marc pypaert suggested.
this is going back to the original paper on UA staining by stempek and
ward. it is certainly outlined in dan pease's book, where en bloc
staining with UA is a solution for membrane extraction. the
explaination was that the UA not only stained, it stabilized the
internal membranes by addition of density so that they were not
extracted by ethanol. in a sense it is a form of fixation. boy, do
people hate me when i say that.

now i use both wherever possible and get perfect membranes. when i
can't, eg LR white, i use UA and have no problems.

unfortunately, it is a matter of old literature which has been lost
today. some of the old books from the '60's still have very good basic
information. hayat's first edition of principles and techniques is
still excellent, and his two books on 1. fixation and 2. positive
staining are, sadly, long out of print. i am lucky to have both, and
they are borrowed regularly by students.

give it a shot with the UA and then the acetone. if that does not work,
give me a call. i can ignore you as easily as i do my own department
chair...

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 07:38:04 2005



From: Gerd Leitinger :      gerd.leitinger-at-meduni-graz.at
Date: Wed, 01 Jun 2005 14:33:59 +0200
Subject: [Microscopy] Re: viaWWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Where do your cells grow?
I had problems with osmium fixation of cell cultures when they grew on
plastic membranes, and a nice lady from this list server suggested that
I should reduce the osmium tetraoxide with potassium ferrocyanide
because the osmium reacts with the plastic polymer.

We now grow the cells on "Aclar film" and get good membrane
contrast, with or without reducing the osmium tetraoxide. This film is
supposed to be chemically insensitive and can withstand dehydration
and embedding.
The film has to be pre-treated with poly L lysine to prevent the cells
from floating away, though.

yours

Gerd Leitinger


}
} Question: I have recently been having problems getting good
staining of the membranes of cultured cells with osmium. The cells
were fixed with 2% glutaraldehyde and post fixed in 1% osmium
tetroxide. I have tried 0.1M phosphate buffer, pH 7.4 and 0.1M
cacodylate buffer pH 7.3. When using these the membranes were not
visible at all. I also tried osmium with potassium ferrocyanide which
worked well with phosphate buffer but left a black precipitate. With
cacodylate buffer and potassium ferrocyanide the membrane definition
was a little better but still not adequate. If anyone has any suggestions
as to how to improve the membrane staining, they would be greatly
appreciated. Thank you.
}
} ---------------------------------------------------------------------------
}




Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 07:48:37 2005



From: Gerd Leitinger :      gerd.leitinger-at-meduni-graz.at
Date: Wed, 01 Jun 2005 14:44:31 +0200
Subject: [Microscopy] Methanol permeabilisation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

I have a question that takes up the recent permeabilisation issue:

Is it possible to permeabilise membranes with methanol instead of
ethanol for pre-embedding immunoEM, and what effects does the
methanol have on the ultrastructure of membranes?

I have some experience with pre-embedding immunocytochemistry of
vibratome slices of nervous tissue. For this, I usually pre-treat the cells
with up to 50% ethanol for a few minutes, and then add 0.05% saponin
to all the antibody solutions, and usually get quite good staining in the
outer regions of the slices.

I now wanted to do a similar procedure on cultivated cells and have
found that the antibodies do not penetrate into the cells at all! For light
microscopic immunocytochemistry, I have found that the antibodies
only penetrate if these cells are pre-treated with 100% methanol. For
immunoEM, I only tested my "usual" protocol of 10, 30, and 50%
ethanol for a few minutes each. Does anybody know whether it is worth
trying methanol for immunoEM, and perhaps applying the methanol for
a longer time, perhaps 30 minutes?

thank you

Gerd Leitinger


Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 08:17:07 2005



From: Aleksandr Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Wed, 01 Jun 2005 14:16:37 +0100
Subject: [Microscopy] Re: Methanol permeabilisation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Gerd,

Ethanol and methanol will destroy all the membranes you have in the
sample. You will see only some blobs of different densities instead of
nicely preserved cells.
For pre-embedding EM on cultured cells people use saponin 0.1% or
digitonin (do not remember %), even Triton X-100 is too harsh.

Gerd Leitinger:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 08:52:01 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Wed, 01 Jun 2005 08:51:18 -0500
Subject: [Microscopy] Re: viaWWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Robert,

Try adding 1% tannic acid to your glut and possibly the OsO4,
Mallinckrodt cat. #1764 seems to work best -- I believe because it's
a monomer, instead of various polymers.
This helps with the membrane preservation.
Is this for TEM or SEM?

Phil

} Email: rtemkin-at-mtsinai.on.ca
} Name: Robert Temkin
}
} Organization: Hospital for Sick Children Research Institute, Toronto, Canada
}
} Title-Subject: [Microscopy] [Filtered] osmium fixation of cultured cells
}
} Question: I have recently been having problems getting good staining
} of the membranes of cultured cells with osmium. The cells were fixed
} with 2% glutaraldehyde and post fixed in 1% osmium tetroxide. I have
} tried 0.1M phosphate buffer, pH 7.4 and 0.1M cacodylate buffer pH
} 7.3. When using these the membranes were not visible at all. I also
} tried osmium with potassium ferrocyanide which worked well with
} phosphate buffer but left a black precipitate. With cacodylate
} buffer and potassium ferrocyanide the membrane definition was a
} little better but still not adequate. If anyone has any suggestions
} as to how to improve the membrane staining, they would be greatly
} appreciated. Thank you.
}
} ---------------------------------------------------------------------------

--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 10:10:03 2005



From: Gretchen.Ziegler-at-leica-microsystems.com
Date: Wed, 1 Jun 2005 10:09:02 -0500
Subject: [Microscopy] position open at West Chester University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Hello All,

I am posting this as a favour to Dr. Helen Reid at West Chester University:

The university is looking for a chemical microscopist for the Fall 2005
semester. If you are looking, or know anyone who is looking, for this
type of position, please contact Helen directly at:

hreid-at-wcupa.edu

Thanks,

Gretchen




Gretchen Ziegler, Sales Manager, Educational Division
Leica Microsystems, Inc.
P.O. Box 151 - Ocean Grove, NJ 07756
Phone: 732-897-9506 - Fax: 847-236-3013
Voicemail: 1-800-248-0665 ext. 5131
www.DiscoverMicroscopy.com


_____________________________________________________________________
This e-mail has been scanned for viruses by MCI's Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.mci.com


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 11:24:53 2005



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Wed, 1 Jun 2005 12:06:40 -0400
Subject: [Microscopy] Re: via WWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,
} It is really something to see one's name in print, especially
} for being the person to go to for information on a
} particular technique! Thanks Paul.
}
} With tissue cultured cells I have found it critical to fix them as
} soon as possible after they come out of the incubator.
} DO NOT WASH them! Simply decant the growth
} medium then immediately and gently flood the cells with
} the fixative.
}
} The fixative that works well on many different types of cultured
} cells as well as pelleted material and small tissue pieces is as follows:
} 1% Glutaraldehyde+1% Osmium tetroxide+0.05 M Phosphate Buffer
} -at-6.2 pH on ice and kept dark. Usually 45 minutes is fine.
}
} Yes, this fixative will start to oxidize. To avoid this have all
} the components
} on ice; mix in the glutaraldehyde immediately before the fixative is needed
} and allow sufficient volume to fix both the cells and the small amount of
} protein that is left on the cells after decanting the medium.
}
} Dr. Tilney INSISTS that the Electron Microscopy Sciences
} 8% Glutaraldehyde in the 10 ml vials be used
} at all times for his actin studies and that it be kept in
} a scintillation vial and discarded one week after opening
} the vial. The only reason for using the EMS product is that
} we always get excellent results and he refuses to even try
} another company.
}
} With the phosphate buffer one needs to wash well with cold
} (best available) water at least 3 times over 20 - 30 min. time
} AND remember to rinse the entire vessel (top too)
} at least once before adding cold 1% UA in water
} overnight in the refrigerator to avoid the dreaded uranyl-phosphate
} crystals.There is no light in our refrigerator so I do not need to worry
} if it really goes out when the door is closed.
}
} If the cells are to be fixed in the flask/petri dish for face-on sections,
} an ethanol dehydration is used and Ladd's LX-112 as an epon
} substitute. These do not melt the plastic.
} All other cases or cells grown in "Pernanox" dishes
} are acetone dehydrated and any epon substitute can be used.
}
} Contact me off-line if more information is needed.
} If there are several similar questions I'll post them.
}
} If this fixation does not show what you wish with the membranes
} try using an objective apperature that is a size smaller than is
} usually used in the TEM.
}
} Pat Connelly psconnel-at-sas.upenn.edu
} Dept. of Biology, University of Pennsylvania
} Philadelphia, PA 19104-6018


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 12:12:10 2005



From: Dave Joswiak :      joswiak-at-astro.washington.edu
Date: Wed, 1 Jun 2005 10:11:28 -0700 (PDT)
Subject: [Microscopy] looking for datacube software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would be interested in hearing from the microscopy community what 3rd
party software is available for looking at datasets obtained from spectrum
imaging. As many of you know, spectrum images are 3d datasets which
contain an EDX (or EELS) spectrum at each pixel from a STEM image. We
have a Tecnai F20 FEG with STEM/EDX/EELS and want to probe deeper into
extracting useful information from these datacubes; our software can
generate basic element maps but we want to purchase software that is more
flexible and comprehensive and perhaps interactive too. For instance, we
would like to be able to highlight a subset of pixels in an image or
element map and see these points appear in a ternary diagram or other type
of plot. We can generate a spreadsheet of quantified elements or element
ratios for each pixel in a STEM image and can export the spreadsheet.
Thanks for your input.

Dave Joswiak
Univ. of Washington
Dept. of Astronomy
Seattle, WA


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 13:23:25 2005



From: Greg Erdos :      gwe-at-ufl.edu
Date: Wed, 01 Jun 2005 14:22:41 -0400
Subject: [Microscopy] Fwd: (Fwd) hawaii conference and LIMS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am forwarding this on behalf of Mr. Goodblatt.. He would like to have an
informal session at M&M in Honolulu regarding items he has
mentioned. Please reply to him directly if you are interested.





} ------- Forwarded message follows -------
} From: Avrum Goodblatt {goodblat-at-mail.med.upenn.edu}
} To: gwe-at-ufl.edu
} Subject: [Microscopy] hawaii conference and LIMS
} Copies to: luellen-at-mail.med.upenn.edu, jonni S. Moore
} {moorej-at-mail.med.upenn.edu} ,
} qcyu-at-mail.med.upenn.edu
} Date sent: Thu, 26 May 2005 12:01:48 -0400
}
}
} Managing data today has become more challenging due to the very large
} datasets
} being produced. It is not uncommon for a facility to turn out a full DVD
} of data in one
} day. Providing online access and backup becomes more complicated, especially
} when one also has to protect instrumentation from viruses and other attacks.
}
} In addition, usage and billing records should be tied into the LIMS but
} frequently
} these are two completely different systems. With compliance much more on
} the radar
} today, and funds coming from a wider variety of sources, better
} record-keeping is
} essential
}
} I am IT coordinator for a group of 9 resource facilities called
} PathBioResource
} (including BioMedical Imaging) in Pathology and Laboratory Medicine at the
} University of Pennsylvania. I would like to share with others what we are
} doing and
} learn from others ways to approach these issues. We might also find ways
} to share
} some tool development together in some open source fashion, or educate
} vendors as
} to what is needed.
}
} I will be in Honolulu at the conference from Sunday evening through Wednesday
} evening.
}
} Avrum Goodblatt
} goodblat-at-mail.med.upenn.edu
} 215 573 0675
}
}
}
} ------- End of forwarded message ---------
} Avrum Goodblatt goodblat-at-mail.med.upenn.edu
} BMCRC 215 573 0675

Gregory W. Erdos Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
352-392-1295
fax- 352-846-0251



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 13:48:41 2005



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 01 Jun 2005 13:47:53 -0500
Subject: [Microscopy] Re: looking for datacube software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I hope you are already familiar with the LISPIX package available through
NIST (http://www.nist.gov/lispix/doc/contents.htm). It is free and should
allow you to play with your data as you please. I have not used it myself
so I cannot comment on it.

The other question will be the data format. I don't know that vendors
afford export into a generic LISPIX format in a similar way as EDS data can
be exported to MSA format. Hopefully such portability will be forthcoming.
You can take the matter up with your EDS or EELS vendor.

Warren

At 12:11 PM 06/01/05, you wrote:

} I would be interested in hearing from the microscopy community what 3rd
} party software is available for looking at datasets obtained from spectrum
} imaging. As many of you know, spectrum images are 3d datasets which
} contain an EDX (or EELS) spectrum at each pixel from a STEM image. We
} have a Tecnai F20 FEG with STEM/EDX/EELS and want to probe deeper into
} extracting useful information from these datacubes; our software can
} generate basic element maps but we want to purchase software that is more
} flexible and comprehensive and perhaps interactive too. For instance, we
} would like to be able to highlight a subset of pixels in an image or
} element map and see these points appear in a ternary diagram or other type
} of plot. We can generate a spreadsheet of quantified elements or element
} ratios for each pixel in a STEM image and can export the spreadsheet.
} Thanks for your input.
}
} Dave Joswiak
} Univ. of Washington
} Dept. of Astronomy
} Seattle, WA

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 13:57:51 2005



From: Christine Weaver, McRI :      cweaver-at-mcri.org
Date: Wed, 1 Jun 2005 13:57:00 -0500
Subject: [Microscopy] Inter/Micro 2005 Preliminary Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Inter/Micro 2005 Preliminary Program
July 11-15, 2005
Talbott Hotel & McCrone Research Institute
Chicago, Illinois

McCrone Research Institute (McRI) cordially invites you to join us at
Inter/Micro 2005, an internationally recognized professional meeting
dedicated to applied microscopy, held each year in downtown Chicago.

The Inter/Micro 2005 conference will span 5 days. The first 3 days of the
conference will consist of a symposium, to be held at the Talbott Hotel,
featuring daily 15-20 minute paper presentations, a Monday evening session
with Brian J. Ford, Exhibitors (Tuesday and Wednesday), and McRI’s joint
meeting with the State Microscopical Society of Illinois including a
Banquet, Awards Ceremony, and Auction (Wednesday Evening). The symposium
will be followed by a special 2-day workshop (July 14th and 15th), to be
held at the laboratories and classrooms of McCrone Research Institute,
entitled "Introduction to Techniques of Forensic Soil Comparison" conducted
by Skip Palenik. Exhibitors and sponsor’s for Inter/Micro 2005 include:
Leica Microsystems, Educational Products, McCrone Research Institute, Bruker
Optics, Lomo America, State Microscopical Society of Illinois, Olympus
America, and Tienta Sciences.

To exhibit or sponsor (deadline July 1, 2005) please visit:
http://mcri.org/ExhibitorINFOandCONTRACT.pdf

The papers presented at Inter/Micro 2005 will be published in the
International Journal on microscopy, The MICROSCOPE. For more information,
call 312-842-7100 visit www.mcri.org or contact intermicro-at-mcri.org.


Monday, July 11
Techniques and Instrumentation

8:00 a.m. Registration: 1st floor, Gallery
9:00 a.m. Symposium: 1st floor, Delaware Room

Laughlin, Gary – McCrone Research Institute (McRI), Welcome

Havics, Tony – QEPI, A Menagerie of Microscopy

Weaver, Robert – McRI, Techniques of Controlling Depth of Field

Hinsch, Jan – Leica Microsystems, Modes of Viewing the Microscope’s Back
Focal Plane

Weaver, Robert – McRI, 40 Years of the Microscope Tricks of the Trade

Ford, Brian J. – Gonville & Caius College, University of Cambridge, A
Century of Brownian Motion

12:00 p.m. – 2:00 p.m. Lunch

Hinsch, Jan – Leica Microsystems, The Flatbed Scanner: Useful Beyond
Document Work

Erdman, Natasha – JEOL USA, Preparation of Clean Cross Sections Using JEOL
Cross Sectional Polisher (CP)

Bayard, Michael – Bayard Development, Microscopy of Solid Solution,
Co-Crystallized and Multi-Component Systems

Barnes, C.G. – Dow Chemical Company, Selective Etching of Nylon in Nylon/sPS
Fibers for Determination of sPS Morphology

Palenik, Mark – Microtrace, Characterization of Soot and Carbon Black by GC
and TEM

Sparenga, Sebastian – McRI, Identification of Abrasives in Smokeless Tobacco

8:00 p.m. Brian J. Ford, “Evening with Brian – Talk the Talk”


Tuesday, July 12
Environmental and Industrial Microscopy

8:00 a.m. Registration: 1st floor, Gallery
9:00 a.m. Symposium: 1st floor, Delaware Room

Bucher, Edith – Laboratorio Biologico - APPA Bolzano, Applied Aerobiology
and Melissopalynology in South Tyrol (Northern Italy) - Realization of a
Pollen Atlas

Chun-I Lee, James – University of Strathclyde, Diatom Distribution in
Danshuei River

Laughlin, Gary – McRI, Indoor Air Quality: Microscopy of House Dust
Particles

Speir, Jacqueline – McRI, Can Dispersion Aid in Amphibole Differentiation?

Chatfield, Eric – Chatfield Technical Consulting, TBA

Havics, Tony – QEPI, Asbestos: To Be a Fiber or Not To Be

12:00 p.m. – 2:00 p.m. Lunch

Ford, Brian J. – Gonville & Caius College, University of Cambridge, The
Discovery of Giardia

Alden, Harry – Smithsonian Center for Materials Research and Education, Hair
of the Dog, or How Blanket Fibers Can Get Your Goat

Welsh, Frank– Welsh Color and Conservation, Inc., Historic Paint Colors of
Spanish St. Augustine

Hills, Linda – CTL Group, From Rocks to Bridges: An Introduction to Cement
and Concrete Microstructure

Hagni, Ann – CTL Group, Failure Analyses of Construction Materials Using
Optical Microscopy

Rantanen, Walter – Integrated Paper Services, Inc., Matching Matches (Part
2)

5:00 p.m. – 6:00 p.m. Cocktail Hour with Exhibitors: 2nd floor, Victoria
Room

Wednesday, July 13
Chemical and Forensic Microscopy

8:00 a.m. Registration: 1st floor, Gallery
9:00 a.m. Symposium, 1st Floor Delaware Room

Crowe, John – FDA Forensic Chemistry Center, The Characterization of
Microchemical Test Resultant Crystalline Formations using PLM, FT-IR and
Raman Spectroscopy

Bowen, Andrew – Stoney Forensic, Inc., Recent Contributions of Chemical
Microscopy to the Analysis of Unknowns

Rothhaar, Katia – Tienta Sciences, Use of Drop Coated Deposition Raman for
Detection of Explosives

Hollifield, Jeff – Micro Analytical, Flow Chart for Rapid Identification of
Inorganic Compounds Using PLM

Ford, Brian – Gonville & Caius College, University of Cambridge, World's
Worst Microscopy

Palenik, Skip – Microtrace, TBA

12:00 p.m. – 2:00 p.m. Lunch

Reffner, John – Smith’s Detection, TBA

Boltin, Randy – MVA Scientific Consultants, A Class in Forensic Microscopy

Lawrence, Gene – San Diego County Sheriff's Crime Lab, Marine Coatings -
Components and Analysis

Grant, Ricky – 41st CST (WMD) US ARMY, Active National Guard, Weapons Of
Mass Destruction Terrorism Experts and Forensic Microscopy

Hopen, Thomas – ATF Forensic Science Laboratory, PLM Characterization of
Inorganic Constituents in Automobile Body Fillers

Hietpas, Jack – Microtrace, Microscopy of Explosives

Diaczuk, Peter – John Jay College of Criminal Justice, Determination of
Entry vs. Exit Bullet Holes in Garments using Light Microscopy

6:00 p.m. SMSI Banquet and Auction, Victoria Room
Dr. Osamu Shimomura, SMSI 2005 Émile Chamot
Award Recipient

Regards,
Christine

Christine Weaver
Inter/Micro Coordinator
McCrone Research Institute
2820 S. Michigan Avenue
Chicago IL 60616

312-842-7100
cweaver-at-mcri.org
http://www.mcri.org





From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 15:01:35 2005



From: Tea Meulia :      meulia.1-at-osu.edu
Date: Wed, 1 Jun 2005 16:02:43 -0400
Subject: [Microscopy] intestine em immunolocalizations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We would like to do some em immunolocalizations on pig intestinal
tissue to detect virus infected cells. Can anyone direct me to some
literature? Which would be the best way to start pre- or
post-embedding labeling?

The antibodies, we will be using, work well in confocal microscopy
on whole tissue with Triton permeabilization, or on paraffin sections

Thank you.

Tea Meulia




--
***************************************
Tea Meulia, PhD
Director
Molecular and Cellular Imaging Center
Ohio State University/OARDC
1680 Madison Ave.
Wooster OH 44691

tel.: 330-263-3836 or -3828
fax: 330-202-3563

http://www.oardc.ohio-state.edu/mcic

*****************************************


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 15:17:01 2005



From: Elaine Humphrey :      ech-at-interchange.ubc.ca
Date: Wed, 01 Jun 2005 13:16:14 -0700
Subject: [Microscopy] gaston naessens and somatid biology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone
I have just had a request for the use of a somatoscope developed by
Gaston Naessens as they want colour at 30,000 times.

I was wondering if someone was pulling my leg but when I did a google
search I got
http://www.sumeria.net/tech/naessens.html
and I am still wondering if someone has a huge hoax going. Has anyone
got experience with this type of microscope?

Elaine


--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada (2003-2005)
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 16:22:14 2005



From: rtemkin-at-mtsinai.on.ca (by way of MicroscopyListserver)
Date: Wed, 1 Jun 2005 16:21:32 -0500
Subject: [Microscopy] viaWWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (rtemkin-at-mtsinai.on.ca) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, June 1, 2005 at 08:06:18
---------------------------------------------------------------------------

Email: rtemkin-at-mtsinai.on.ca
Name: Robert Temkin

Organization: Hospital for Sick Children Research Institute, Toronto, Canada

Title-Subject: [Microscopy] [Filtered] osmium fixation of cultured cells

Question: Thanks for the responses but I guess I did leave out some details. I'm processing monolayers grown on glass coverslips. I do use en bloc staining with uranyl acetate. I'm using 2% aqueous UA for 45-60 minutes at room temperature. I use similar times for the glutaraldehyde and osmium fixations. I dehydrate with ethanol and embed in Epon. I separate the coverslip from the plastic with liquid nitrogen. The bizarre thing is that this protocol has worked well for the past few years but recently has given poor results. I'll try some of the suggestions but I'm wondering if the en bloc UA needs to be done overnight since I'm dealing with monolayers.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 16:22:48 2005



From: kevin.selkregg-at-us.vesuvius.com (by way of MicroscopyListserver)
Date: Wed, 1 Jun 2005 16:22:05 -0500
Subject: [Microscopy] viaWWW: Sodium Aluminate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kevin.selkregg-at-us.vesuvius.com) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, June 1, 2005 at 13:30:05
---------------------------------------------------------------------------

Email: kevin.selkregg-at-us.vesuvius.com
Name: Kevin Selkregg

Title-Subject: [Microscopy] [Filtered] thermal expansion coefficient

Question: Even though this question does not appear microscopy related; the thermal expansion in this situation definitely affects the microstructure of the parent material I have been studying.

Anyone have any thermal expansion coefficient data for Sodium Aluminate. I am looking specifically for the coefficient values for NaAlO2 (1 Na2O : 1 Al2O3).

Thank you.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 16:34:41 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Wed, 1 Jun 2005 16:33:59 -0500
Subject: [Microscopy] NIST-MAS-AMAS VPSEM-ESEM Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Following Workshop Meeting Announcement which was posted on the MAS Listserver may also be of interest
to the Microscopy Listserver readers.

Nestor.
Your Friendly Neighborhood SysOp

*****************************************************************************

NIST-MAS-AMAS Workshop on Variable Pressure and Environmental Scanning
Electron Microscopy (VPSEM-ESEM) for Imaging and Microanalysis: Roadmap 3
{http://www.nist.gov/esem}

Place: Lecture Room A and Poster Hall
National Institute of Standards and Technology,
100 Bureau Drive, Gaithersburg, Maryland 20899
Dates: November 2 4, 2005

Co-organizers:
NIST Surface and Microanalysis Science Division (837)
NIST Precision Engineering Division (821)
Microbeam Analysis Society (MAS) {http://www.microbeamanalysis.org/}
Australian Microbeam Analysis Society (AMAS) {http://www.microscopy.org.au/}

From November 2 to 4, 2005, NIST will co-host a workshop on Variable
Pressure Scanning Electron Microscopy (VPSEM) and Environmental SEM (ESEM)
in conjunction with the Microbeam Analysis Society (MAS) and the Australian
Microbeam Analysis Society (AMAS). Building on the highly successful AMAS
Roadmap 1 and 2 workshops on VPSEM-ESEM organized by Brendan Griffin, this
VPSEM-ESEM workshop, which constitutes Roadmap 3, will provide an
opportunity for technical presentations (platform and poster),
demonstrations, and discussions on leading topics in microscopy and
microanalysis in VPSEM-ESEM. Special attention will be given to new
breakthrough areas, such as combined VPSEM-ESEM-FIB (focused ion beam)
instrumentation. Discussions will attempt to identify those critical
issues where research is needed for further progress in VPSEM-ESEM.
The program as it has been currently developed is attached below. We are
seeking interested participants, proposed titles for presentations, as well
as suggestions for additional topics and invited keynote speakers to
include. In keeping with past NIST-MAS workshops, there will be no cost
for attendance, but pre-registration is required so that the available
lecture room space is not overwhelmed and NIST security requirements can be
satisfied.

NIST-MAS-AMAS VPSEM-ESEM Workshop
Tentative Program
Wednesday, Nov 2, 2005

AM: Electron and ion interactions in the gas environment
Organizer: Brad Thiel (SUNY-Albany)
Topics: fundamentals, charge control, radiation damage

PM: Contrast mechanisms in VPSEM-ESEM
Organizer: David Joy (Univ. Tennessee) and Brendan Griffin (U. Western
Australia)

Thursday, Nov. 3, 2005
AM: VPSEM-ESEM breakthrough areas:
1. Critical dimension VPSEM-ESEM microscopy
Organizer: Mike Postek/Andras Vladar (NIST)
2. VPSEM-ESEM FIB
Organizer/Invited Speaker: Lucille Giannuzzi

PM: X-ray microanalysis in the VPSEM-ESEM
Organizer: Dale Newbury (NIST)
Invited speaker: Eric Doehne (Getty Conservation Laboratory)

PM: Friday, Nov. 4, 2005
AM: Dynamic experiments in VPSEM-ESEM
Organizer: Scott Wight (NIST)

PM: Roadmap 3: Critical issues in VPSEM-ESEM
Organizer: Brendan Griffin (U. Western Australia)

Please contact dale.newbury-at-nist.gov


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 16:50:06 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Wed, 01 Jun 2005 17:49:03 -0400
Subject: [Microscopy] Re: Re: via WWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Pat,

Funny you should mention fixation with a mixture of
glutaraldehyde and paraforrmaldehyde. I just came
out of the scope where I looked at some samples of
drosophila egg chambers fixed with such a mixture,
followed by overnight incubation in uranyl acetate
(0.5%). Membrane contrast was unbelievable, but
also the preservation of cytoplasmic elements such
as ribosomes, actin filaments and clathrin coats. Why
don't we use that technique for all our EM preps?!

Marc

On Wednesday, June 1, 2005, at 12:06 PM, Pat Connelly wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Greetings,
} } It is really something to see one's name in print, especially
} } for being the person to go to for information on a
} } particular technique! Thanks Paul.
} }
} } With tissue cultured cells I have found it critical to fix them as
} } soon as possible after they come out of the incubator.
} } DO NOT WASH them! Simply decant the growth
} } medium then immediately and gently flood the cells with
} } the fixative.
} }
} } The fixative that works well on many different types of cultured
} } cells as well as pelleted material and small tissue pieces is as
} } follows:
} } 1% Glutaraldehyde+1% Osmium tetroxide+0.05 M Phosphate Buffer
} } -at-6.2 pH on ice and kept dark. Usually 45 minutes is fine.
} }
} } Yes, this fixative will start to oxidize. To avoid this have all the
} } components
} } on ice; mix in the glutaraldehyde immediately before the fixative is
} } needed
} } and allow sufficient volume to fix both the cells and the small
} } amount of
} } protein that is left on the cells after decanting the medium.
} }
} } Dr. Tilney INSISTS that the Electron Microscopy Sciences
} } 8% Glutaraldehyde in the 10 ml vials be used
} } at all times for his actin studies and that it be kept in
} } a scintillation vial and discarded one week after opening
} } the vial. The only reason for using the EMS product is that
} } we always get excellent results and he refuses to even try
} } another company.
} }
} } With the phosphate buffer one needs to wash well with cold
} } (best available) water at least 3 times over 20 - 30 min. time
} } AND remember to rinse the entire vessel (top too)
} } at least once before adding cold 1% UA in water
} } overnight in the refrigerator to avoid the dreaded uranyl-phosphate
} } crystals.There is no light in our refrigerator so I do not need to
} } worry
} } if it really goes out when the door is closed.
} }
} } If the cells are to be fixed in the flask/petri dish for face-on
} } sections,
} } an ethanol dehydration is used and Ladd's LX-112 as an epon
} } substitute. These do not melt the plastic.
} } All other cases or cells grown in "Pernanox" dishes
} } are acetone dehydrated and any epon substitute can be used.
} }
} } Contact me off-line if more information is needed.
} } If there are several similar questions I'll post them.
} }
} } If this fixation does not show what you wish with the membranes
} } try using an objective apperature that is a size smaller than is
} } usually used in the TEM.
} }
} } Pat Connelly psconnel-at-sas.upenn.edu
} } Dept. of Biology, University of Pennsylvania
} } Philadelphia, PA 19104-6018
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 16:53:58 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Wed, 01 Jun 2005 16:53:14 -0500
Subject: [Microscopy] Re: gaston naessens and somatid biology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

gee elaine, my first reaction was that this is an interesting question,
and that i'm sure that speculation as to this new instrumentation will
be running rife over your question. after checking the webpage you
referred to, i would even say the speculation will be royal....

i couldn't help that - please, don't ask me to apologize.... the devil
made me do it, ok....

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 17:11:51 2005



From: JoAnn Buchanan :      redhair-at-stanford.edu
Date: Wed, 01 Jun 2005 15:11:07 -0700
Subject: [Microscopy] osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Robert. You have gotten some good suggestions so far. I might add
a few things:
You should try the reduced osmium- potassium ferricyanide with cacodylate
buffer instead of phosphate and you can get rid of the ppt problem.
Leave your cells in serum until you are ready to fix and keep them warm
until ready to fix(right out of incubator). Sick cells will never look good
no matter what you do.
I embed cultured hippocampal neurons in Chang embedding molds then dissolve
away the whole glass coverslip after embedding. Dissolve the coverslip with
hydrofluoric acid(under hood)- that way you have the whole coverslip and
lots of cells. If the coverslips are coated with matrigel they are harder
to work with the nitrogen method.

For the thin sections, I stain in 5% aqueous UA a for 20 minutes or longer.
Follow with a shorter(1-2 mins) in fresh lead stain. I do not do a
prolonged UA stain en bloc-I microwave everything.
Use a lower kV or smaller aperture to image cells.
I would be happy to send you a detailed protocol.
Good luck, JoAnn

Question: Thanks for the responses but I guess I did leave out some
details. I'm processing monolayers grown on glass coverslips. I do use en
bloc staining with uranyl acetate. I'm using 2% aqueous UA for 45-60
minutes at room temperature. I use similar times for the glutaraldehyde and
osmium fixations. I dehydrate with ethanol and embed in Epon. I separate
the coverslip from the plastic with liquid nitrogen. The bizarre thing is
that this protocol has worked well for the past few years but recently has
given poor results. I'll try some of the suggestions but I'm wondering if
the en bloc UA needs to be done overnight since I'm dealing with monolayers.



Department of Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 17:19:37 2005



From: Webster, Paul :      PWebster-at-hei.org
Date: Wed, 01 Jun 2005 15:16:16 -0700
Subject: [Microscopy] Re: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is reduced osmium prepared with ferrocyanide or ferricyanide? I know which
one I use.

Regards,

Paul Webster.




On 6/1/05 3:11 PM, "JoAnn Buchanan" {redhair-at-stanford.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Dear Robert. You have gotten some good suggestions so far. I might add
} a few things:
} You should try the reduced osmium- potassium ferricyanide with cacodylate
} buffer instead of phosphate and you can get rid of the ppt problem.
} Leave your cells in serum until you are ready to fix and keep them warm
} until ready to fix(right out of incubator). Sick cells will never look good
} no matter what you do.
} I embed cultured hippocampal neurons in Chang embedding molds then dissolve
} away the whole glass coverslip after embedding. Dissolve the coverslip with
} hydrofluoric acid(under hood)- that way you have the whole coverslip and
} lots of cells. If the coverslips are coated with matrigel they are harder
} to work with the nitrogen method.
}
} For the thin sections, I stain in 5% aqueous UA a for 20 minutes or longer.
} Follow with a shorter(1-2 mins) in fresh lead stain. I do not do a
} prolonged UA stain en bloc-I microwave everything.
} Use a lower kV or smaller aperture to image cells.
} I would be happy to send you a detailed protocol.
} Good luck, JoAnn
}
} Question: Thanks for the responses but I guess I did leave out some
} details. I'm processing monolayers grown on glass coverslips. I do use en
} bloc staining with uranyl acetate. I'm using 2% aqueous UA for 45-60
} minutes at room temperature. I use similar times for the glutaraldehyde and
} osmium fixations. I dehydrate with ethanol and embed in Epon. I separate
} the coverslip from the plastic with liquid nitrogen. The bizarre thing is
} that this protocol has worked well for the past few years but recently has
} given poor results. I'll try some of the suggestions but I'm wondering if
} the en bloc UA needs to be done overnight since I'm dealing with monolayers.
}
}
}
} Department of Molecular and Cellular Physiology
} Stanford University School of Medicine
} Stanford, CA 94305
} 650-723-5856
}
}



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 17:27:10 2005



From: Tamara Howard :      thoward-at-unm.edu
Date: Wed, 1 Jun 2005 16:26:28 -0600 (MDT)
Subject: [Microscopy] Re: gaston naessens and somatid biology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Odd that there aren't any images associated with the websites lauding
Naessens, don't you think? At least, not on any of the first page of
Google hits I came up with.

Tamara

On Wed, 1 Jun 2005, Elaine Humphrey wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hello everyone
} I have just had a request for the use of a somatoscope developed by Gaston
} Naessens as they want colour at 30,000 times.
}
} I was wondering if someone was pulling my leg but when I did a google search
} I got
} http://www.sumeria.net/tech/naessens.html
} and I am still wondering if someone has a huge hoax going. Has anyone got
} experience with this type of microscope?
}
} Elaine
}
}
} --
} Dr. Elaine Humphrey
} Director, BioImaging Facility
} President, Microscopy Society of Canada (2003-2005)
} University of British Columbia
} 6270 University Blvd, mail-stop Botany
} Vancouver, BC
} CANADA, V6T 1Z4
} Phone: 604-822-3354
} FAX: 604-822-6089
} e-mail: ech-at-interchange.ubc.ca
} website: www.emlab.ubc.ca
}
}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 17:32:28 2005



From: Christopher Gilpin :      christopher.gilpin-at-utsouthwestern.edu
Date: Wed, 1 Jun 2005 17:31:43 -0500
Subject: [Microscopy] Re: gaston naessens and somatid biology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Elaine,
To save another exile from speculation ( Royal or otherwise) I look forward
to a report on the demo when you orgainse it. :)

Chris



Christopher J Gilpin Ph.D.
Assistant Professor
Director, Molecular and Cellular Imaging Facility
K1.A04
UT Southwestern Medical Center at Dallas
5323 Harry Hines Boulevard
Dallas, TX 75390-9039
Phone +1 214 648 2827
Fax +1 214 648 6408

-----Original Message-----
} From: paul r hazelton [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Wednesday, June 01, 2005 4:53 PM
To: Elaine Humphrey
Cc: microscopy-at-msa.microscopy.com

gee elaine, my first reaction was that this is an interesting question, and
that i'm sure that speculation as to this new instrumentation will be
running rife over your question. after checking the webpage you referred
to, i would even say the speculation will be royal....

i couldn't help that - please, don't ask me to apologize.... the devil made
me do it, ok....

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 1 21:20:15 2005



From: Rosemary White :      Rosemary.White-at-csiro.au
Date: Thu, 02 Jun 2005 12:20:31 +1000
Subject: [Microscopy] Re: Re: gaston naessens and somatid biology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hmm, the only new optics I've heard about recently, also from Canada, is to
do with the liquid crystal thin lenses developed by Galstian and Presnyakov

Vladimir V. Presnyakov, Karen E. Asatryan, and Tigran Galstian (2002)
Polymer-stabilized liquid crystal for tunable microlens applications.
Optics Express 10: 865 - 870

Vladimir V. Presnyakov and Tigran V. Galstian (2005) Electrically tunable
polymer stabilized liquid-crystal lens. J. Appl. Phys. 97: 103101


} From: Tamara Howard {thoward-at-unm.edu}
} Date: Wed, 1 Jun 2005 16:26:28 -0600 (MDT)
} To: Elaine Humphrey {ech-at-interchange.ubc.ca}
} Cc: microscopy-at-msa.microscopy.com
} Subject: [Microscopy] Re: gaston naessens and somatid biology
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Odd that there aren't any images associated with the websites lauding
} Naessens, don't you think? At least, not on any of the first page of
} Google hits I came up with.
}
} Tamara
}
} On Wed, 1 Jun 2005, Elaine Humphrey wrote:
}
} }
} }
} }
-----------------------------------------------------------------------------} }
-
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------------
} } --
} }
} } Hello everyone
} } I have just had a request for the use of a somatoscope developed by Gaston
} } Naessens as they want colour at 30,000 times.
} }
} } I was wondering if someone was pulling my leg but when I did a google search
} } I got
} } http://www.sumeria.net/tech/naessens.html
} } and I am still wondering if someone has a huge hoax going. Has anyone got
} } experience with this type of microscope?
} }
} } Elaine
} }
} }
} } --
} } Dr. Elaine Humphrey
} } Director, BioImaging Facility
} } President, Microscopy Society of Canada (2003-2005)
} } University of British Columbia
} } 6270 University Blvd, mail-stop Botany
} } Vancouver, BC
} } CANADA, V6T 1Z4
} } Phone: 604-822-3354
} } FAX: 604-822-6089
} } e-mail: ech-at-interchange.ubc.ca
} } website: www.emlab.ubc.ca
} }
} }
}
} |--------------------------------------------------|
} Tamara Howard
} Department of Cell Biology and Physiology
} University of New Mexico - Health Sciences Center
} Albuquerque, NM 87131
} thoward-at-unm.edu
} |--------------------------------------------------|
}


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 2 04:14:47 2005



From: Allen R. Sampson :      ars-at-sem.com
Date: Thu, 2 Jun 2005 04:13:37 -0500
Subject: [Microscopy] RE: gaston naessens and somatid biology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A total load of crap (forgive my bluntness). Anyone wishing to argue the
point is welcome to do so directly with me, off the listserver (don't reply
to all, just me).

Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
Saint Charles, Illinois 60174
phone (630) 513-7093 fax (630) 513-7092
email mailto:ars-at-sem.com web www.sem.com


On Wednesday, June 01, 2005 3:16 PM, Elaine Humphrey
[SMTP:ech-at-interchange.ubc.ca] wrote:
}
}
}
------------------------------------------------------------------------
------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
-------
}
} Hello everyone
} I have just had a request for the use of a somatoscope developed by
} Gaston Naessens as they want colour at 30,000 times.
}
} I was wondering if someone was pulling my leg but when I did a google
} search I got
} http://www.sumeria.net/tech/naessens.html
} and I am still wondering if someone has a huge hoax going. Has anyone
} got experience with this type of microscope?
}
} Elaine
}
}
} --
} Dr. Elaine Humphrey
} Director, BioImaging Facility
} President, Microscopy Society of Canada (2003-2005)
} University of British Columbia
} 6270 University Blvd, mail-stop Botany
} Vancouver, BC
} CANADA, V6T 1Z4
} Phone: 604-822-3354
} FAX: 604-822-6089
} e-mail: ech-at-interchange.ubc.ca
} website: www.emlab.ubc.ca


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 2 10:56:12 2005



From: Bernie Kestel :      kestel-at-anl.gov
Date: Thu, 02 Jun 2005 10:55:25 -0500
Subject: [Microscopy] High Temperature Bore Scope and Telescope:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A co-worker asked me to assist him with the following problem.
He has metal 3 m.m. diameter discs that are about 0.25 m.m. thick. They
are radioactive due to being neutron irradiated. A fixture has been made
which resembles a punch and die to mechanically deform the discs at 300
degrees centigrade by hydralic pressure applied to a central rod about
1 m.m. In diameter. The "viewing hole" below the disc will be 1.5 m.m.
in diameter by 3 centimeters long or "deep."
What is needed is a telescopic device which can both illuminate the
surface of the disc and transmit an image of it to a viewing
device or camera which is several inches away.

Bernie Kestel
Material Science Division
Argonne National Laboratory
9700 South Cass Avenue
Argonne, Illinois, 60439



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 2 11:02:09 2005



From: Lesley Weston :      leswes-at-shaw.ca
Date: Thu, 02 Jun 2005 09:01:12 -0700
Subject: [Microscopy] Re: gaston naessens and somatid biology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's a lovely idea - the cure for cancer and everything else - and if you
read it fast enough it almost makes sense. And it comes complete with a
conspiracy theory: "Unfortunately, Rife's success attracted the
attention and wrath of the American Medical Association (AMA) and the
powerful pharmaceutical companies - the organised opposition of the medical
fields", which makes it even more authentic.

--
Lesley Weston


} From: Elaine Humphrey {ech-at-interchange.ubc.ca}
} Date: Wed, 01 Jun 2005 13:16:14 -0700
} To: microscopy-at-msa.microscopy.com
} Subject: [Microscopy] gaston naessens and somatid biology
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
--} -
}
} Hello everyone
} I have just had a request for the use of a somatoscope developed by
} Gaston Naessens as they want colour at 30,000 times.
}
} I was wondering if someone was pulling my leg but when I did a google
} search I got
} http://www.sumeria.net/tech/naessens.html
} and I am still wondering if someone has a huge hoax going. Has anyone
} got experience with this type of microscope?
}
} Elaine
}
}
} --
} Dr. Elaine Humphrey
} Director, BioImaging Facility
} President, Microscopy Society of Canada (2003-2005)
} University of British Columbia
} 6270 University Blvd, mail-stop Botany
} Vancouver, BC
} CANADA, V6T 1Z4
} Phone: 604-822-3354
} FAX: 604-822-6089
} e-mail: ech-at-interchange.ubc.ca
} website: www.emlab.ubc.ca
}



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 2 12:10:46 2005



From: Roger Baker :      rcbaker-at-eden.infohwy.com
Date: Thu, 2 Jun 2005 12:09:47 -0500
Subject: [Microscopy] Re: High Temperature Bore Scope and Telescope:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All you likely need is a consumer digital camera that has a video
output for a TV monitor and an small achromatic lens of the right
focal length mounted in front, and most of its surface apertured out
to shield stray light. Some Coolpix cameras are nice because the
front lens surface stays fixed while zooming or focusing.

If you mount a piece of a clean cover slip at the right angle just
outside the hole and then have a bright collimated light beam
reflected into the hole by the tilted glass, there should be little
scattering and your camera setup should get a good hole bottom
picture through the cover glass.

I am sure there are also many more fancy and expensive ways to do the
same job. -- Roger



On Jun 2, 2005, at 10:55 AM, Bernie Kestel wrote:

}
}
} ----------------------------------------------------------------------
} --------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ---------
}
}
} A co-worker asked me to assist him with the following problem.
} He has metal 3 m.m. diameter discs that are about 0.25 m.m.
} thick. They
} are radioactive due to being neutron irradiated. A fixture has
} been made
} which resembles a punch and die to mechanically deform the discs
} at 300
} degrees centigrade by hydralic pressure applied to a central rod
} about
} 1 m.m. In diameter. The "viewing hole" below the disc will be
} 1.5 m.m.
} in diameter by 3 centimeters long or "deep."
} What is needed is a telescopic device which can both
} illuminate the
} surface of the disc and transmit an image of it to a viewing
} device or camera which is several inches away.
}
} Bernie Kestel
} Material Science Division
} Argonne National Laboratory
} 9700 South Cass Avenue
} Argonne, Illinois, 60439
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 2 14:39:47 2005



From: Gazda, Jerzy :      Jerzy.Gazda-at-ceriumlabs.com
Date: Thu, 2 Jun 2005 14:38:58 -0500
Subject: [Microscopy] Announcement - SEM/TEM Semiconductor and Nanotechnology Sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

SEM/TEM Semiconductor and Nanotechnology Sample Prep Workshop
June 7, 2005
Austin, TX

Cerium Laboratories LLC. and Sagitta Inc. will be holding a FREE
workshop to present applications of automated polishing to sample
preparations from semiconductor and nanotechnology materials for SEM,
TEM, and AFM based evaluations.

Two sessions will be held. The morning session will concentrate on SEM
prep, while the afternoon session will be addressing TEM and SRP-AFM
techniques. There will be short presentations and live demonstration of
automated polishing followed by SEM or TEM imaging of these samples
using equipment at CeriumLabs.
A possibility exists for preparation of specimens brought by
participants and imaging sessions on June 8 and 9. Please contact us to
set them up.

For more information or to register, please contact:
Jerzy Gazda - Jerzy.Gazda-at-ceriumlabs.com - 800-538-8450, Ext. 51453; or
Efrat Raz - Efrat.Raz-at-sagitta.com - 408-249-9060.

Please RSVP

Jerzy

******************************************************
Jerzy Gazda, Ph.D. CeriumLaboratories L.L.C.

Supervising Engineer 5204 E. Ben White
Blvd. - MS 512
Austin, TX 78741
TEL: 1-800-538-8450, Ext. 51453
jerzy.gazda-at-ceriumlabs.com
******************************************************





From MicroscopyL-request-at-ns.microscopy.com Thu Jun 2 20:12:34 2005



From: brian.leonard-at-sunhealth.org (by way of MicroscopyListserver)
Date: Thu, 2 Jun 2005 20:11:51 -0500
Subject: [Microscopy] viaWWW: Human leukocyte embedment and thin sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (brian.leonard-at-sunhealth.org) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, June 2, 2005 at 16:33:18
---------------------------------------------------------------------------

Email: brian.leonard-at-sunhealth.org
Name: Brian Leonard

Organization: Sun Health Research Institute

Title-Subject: [Microscopy] [Filtered] Human leukocyte embedment and thin sectioning

Question: Does anyone have a protocol for em processing of buffy coat from human whole blood? I plan on doing post-embedding immuno on thin sections of leukocytes. The ultrastructure of the cells processed with my current method is terrible. I fractionate EDTA-treated whole blood over a Ficoll gradient to get leukocytes, and then fix the buffy coat cells at 4 deg C in 2.5% glut/4% para in 0.1M PB (or pbs). After pelleting, I resuspend the cells in low-melting agarose (37-40 deg) to put the cells in a stable matrix for the remainder of the processing. After dissecting the gel matrix into ~1mm cubes, the remainder of the processing is standard em processing for tissue sections, with final embeddment in L.R. White. Any help is much appreciated. Thanks

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 2 20:12:53 2005



From: lcgould-at-med.cornell.edu (by way of MicroscopyListserver)
Date: Thu, 2 Jun 2005 20:12:10 -0500
Subject: [Microscopy] viaWWW: osmium fixation of cultured cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (lcgould-at-med.cornell.edu) from http://microscopy.com/MLFormMail.html on Thursday, June 2, 2005 at 19:47:23
---------------------------------------------------------------------------

Email: lcgould-at-med.cornell.edu
Name: Leona Cohen-Gould

Organization: Joan & Sanford I. Weill Medical College

Title-Subject: [Microscopy] [Filtered] viaWWW: osmium fixation of cultured cells

Question: Robert,
For membrane preservation I use a fixation protocol given to me years ago by someone who studied photoreceptors (tons of membrane). Her recipes were as follows:
Primary fix: 2.5% glut, 4% pfa and 0.2% picric acid in 0.1M cacodylate buffer, pH 7.3
Post fix: 1% osmium tetrox., 1.5% Pot. ferricyanide (aqueous)

I also en bloc stain with Ur Ac.

for the picric acid (my office of environmental health and safety just loves me) I keep the smallest jar available with the crystals fully covered with water. I use the resultant saturated solution in my primary fix (2 ml picric acid sol'n to 40 ml fix).

The primary fix is based on one published by Somogyi and Takagi in Neuroscience Vol 7 No 7 pp1779-1783, 1982

Lee

--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 2 22:41:53 2005



From: h.cao-at-genesis.co.nz (by way of Ask-A-Microscopist)
Date: Thu, 2 Jun 2005 22:41:10 -0500
Subject: [Microscopy] AskAMicroscopist :fluorescence in skin tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (h.cao-at-genesis.co.nz) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, June 2, 2005 at 22:12:27
---------------------------------------------------------------------------

Email: h.cao-at-genesis.co.nz
Name: Helen Cao

Organization: Genesis R & D Ltd

Education: Graduate College

Location: Auckland, New Zealand

Question: Dear all,

I am having some problems in examining fluorescence in skin tissues. I injected (intradermally ) a fluorescence marker into mouse skin, then fixed skin tissues in 4% PFA at 4C overnight, washed in PBS (3 times, 20min each time at 4C), 20% sucrose at 4C overnight. The skin tissues were then embedded in OCT and frozen over liquid nitrogen. No good sections could be obtained, the microanatomical structures in the sections were fragmented. I tried to freeze fresh skin (unfixed) and obtained very good morphology. Any suggestions on how to preserve the morphology and fluorescence in skin tissues? Can I freeze fresh skin tissues to get good sections, and then fix the tissues before examine the fluorescence? Or, can I fix skin tissues in 4% PFA, get paraffin sections and then examine fluorescence? Do these methods quench the fluorescence?

Thanks for your help in advance


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 08:13:15 2005



From: adriana-at-cab.cnea.gov.ar (by way of MicroscopyListserver)
Date: Fri, 3 Jun 2005 08:12:32 -0500
Subject: [Microscopy] viaWWW: TEM EDS for Philips CM200 UT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (adriana-at-cab.cnea.gov.ar) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, June 3, 2005 at 07:29:20
---------------------------------------------------------------------------

Email: adriana-at-cab.cnea.gov.ar
Name: Adriana CondÛ

Organization: CONICET, Argentina

Title-Subject: [Microscopy] [Filtered] MListserver: TEM EDS for Philips CM200 UT

Question:
Dear microscopists,

We are interested in buying an EDS detector for a Philips CM200 TEM with an ultratwin lens.
We would like to have user experience on the performance of the following EDS systems:

a) Noran System SIX model provided by Thermo Electron, or similar models, that have no shutter, are non-retractable but have automatic FET protection.

b) EDAX Genesis 2000 model, that is non-retractable and has no shutter protection (because of the ultratwin lens).

We are particularly concerned with the damage to the detector when the microscope operates at low magnification. We would like to know whether the detector can be permanently damaged if it receives a high dose of radiation, or if the only consequences are practical (wait until the system decays).

All comments will be appreciated.

Thank you for your help.

Adriana


----------------------------------------------------------
Adriana CondÛ
CONICET Researcher
Metals Physics Group
Centro AtÛmico Bariloche
8400 San Carlos de Bariloche
ARGENTINA
e-mail: adriana-at-cab.cnea.gov.ar
http://www.cab.cnea.gov.ar/cab/invbasica/metales
fax: +54 2944 445299
TE: +54 2944 445290
----------------------------------------------------------


---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 08:42:59 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Fri, 3 Jun 2005 09:42:13 -0400
Subject: [Microscopy] AskAMicroscopist :fluorescence in skin tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I work in a Dermatology/Laser Research Lab and we cut frozen sections of skin
all the time for fluorescent studies and confocal work.

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: by way of Ask-A-Microscopist [mailto:h.cao-at-genesis.co.nz]
Sent: Thursday, June 02, 2005 11:41 PM
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(h.cao-at-genesis.co.nz) from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
Thursday, June 2, 2005 at 22:12:27
---------------------------------------------------------------------------

Email: h.cao-at-genesis.co.nz
Name: Helen Cao

Organization: Genesis R & D Ltd

Education: Graduate College

Location: Auckland, New Zealand

Question: Dear all,

I am having some problems in examining fluorescence in skin tissues. I injected
(intradermally ) a fluorescence marker into mouse skin, then fixed skin tissues
in 4% PFA at 4C overnight, washed in PBS (3 times, 20min each time at 4C), 20%
sucrose at 4C overnight. The skin tissues were then embedded in OCT and frozen
over liquid nitrogen. No good sections could be obtained, the microanatomical
structures in the sections were fragmented. I tried to freeze fresh skin
(unfixed) and obtained very good morphology. Any suggestions on how to preserve
the morphology and fluorescence in skin tissues? Can I freeze fresh skin tissues
to get good sections, and then fix the tissues before examine the fluorescence?
Or, can I fix skin tissues in 4% PFA, get paraffin sections and then examine
fluorescence? Do these methods quench the fluorescence?

Thanks for your help in advance


---------------------------------------------------------------------------




From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 10:11:05 2005



From: frank.karl-at-degussa.com
Date: Fri, 3 Jun 2005 11:30:58 -0400
Subject: [Microscopy] Photoshop 7 vs Photoshop CS2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm looking for some advice. I understand Adobe has issued Photoshop 8 and
called it Photoshop CS2. I am currently using Photoshop 6 with Reindeer
Graphics's Fovea Pro V3. Is anyone actually using Photoshop CS2 and is it
better than Photoshop 7 and will my Povea Pro 3 work with it. I don't
want to be too far behind the curve as technology changes, but I don't want
to be the public debuger either!

Thanks!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient, you are hereby
notified that you must not read this transmission and that any disclosure,
copying, printing, distribution or use of any of the information contained
in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 10:43:44 2005



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Fri, 3 Jun 2005 11:25:44 -0400
Subject: [Microscopy] Re: via WWW: Human leukocyte embedding and thin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Brian:
I have fixed leukocytes that were grown on coverslips but I think that the
same technique may solve your problem.
First: keep PBS away from any cells that you ever wish to do EM on.
This makes a noticeable difference.
LM /confocal investigators do not seem to worry about it.
Second: try fixing the buffy coat at room temperature unless it is already
cold - then cool it down if protocol requires it.
Third: the fixative may work better if it was less concentrated.
1%glutaraldehyde seems to be the top concentration for immuno.
studies but freshly prepared (para)formaldehyde is used in many
labs by itself or in combination with glutaraldehyde.
Others will be sure to remark on this point.
You are welcome,
Pat Connelly
Dept. of Biology
Univ. of Pennsylvania
Philadelphia, PA 19104-6018
psconnel-at-sas.upenn.edu
===================
Below is the result of your feedback form (NJZFM-ultra-55).

Email: brian.leonard-at-sunhealth.org
Name: Brian Leonard
Organization: Sun Health Research Institute
Title-Subject: [Microscopy] Human leukocyte embedment and thin sectioning
Question: Does anyone have a protocol for em processing of buffy coat from
human whole blood? I plan on doing post-embedding immuno on thin sections
of leukocytes. The ultrastructure of the cells processed with my
current method is terrible. I fractionate EDTA-treated whole blood
over a Ficoll gradient to get leukocytes, and then fix the buffy coat
cells at 4 deg C in 2.5% glut/4% para in
0.1M PB (or pbs). After pelleting, I resuspend the cells in low-melting agarose
37-40 deg.) to put the cells in a stable matrix for the remainder of
the processing.After dissecting the gel matrix into ~1mm cubes, the
remainder of
the processing is standard EM processing for tissue sections, with final
embeddment in L.R. White.
Any help is much appreciated. Thanks


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 10:52:07 2005



From: David Elliott :      Elliott-at-arizona.edu
Date: Fri, 3 Jun 2005 08:51:20 -0700
Subject: [Microscopy] Re: Photoshop 7 vs Photoshop CS2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Photoshop 8 came out with CS1. I am using it. I use lots of complex
scripts and the Photoshop 8 scripting was worth my money.
It has lots of other great things, but most of them are of no use to
microscopists (unless you want to forge your data :-)
David


On Jun 3, 2005, at 8:30 AM, frank.karl-at-degussa.com wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} I'm looking for some advice. I understand Adobe has issued Photoshop
} 8 and
} called it Photoshop CS2. I am currently using Photoshop 6 with
} Reindeer
} Graphics's Fovea Pro V3. Is anyone actually using Photoshop CS2 and
} is it
} better than Photoshop 7 and will my Povea Pro 3 work with it. I don't
} want to be too far behind the curve as technology changes, but I don't
} want
} to be the public debuger either!
}
} Thanks!!
}
} Frank Karl
} Degussa Corporation
} Akron Technical Center
} 3500 Embassy Parkway
} Suite 100
} Akron, Ohio 44333
}
}
} 330-668-2235 Ext. 238
}
}
} This e-mail transmission, and any documents, files or previous e-mail
} messages attached to it may contain information that is confidential or
} legally privileged. If you are not the intended recipient, or a person
} responsible for delivering it to the intended recipient, you are hereby
} notified that you must not read this transmission and that any
} disclosure,
} copying, printing, distribution or use of any of the information
} contained
} in or attached to this transmission is STRICTLY PROHIBITED. If you have
} received this transmission in error, please immediately notify the
} sender
} by telephone or return e-mail and delete the original transmission and
} its
} attachments without reading or saving in any manner. Thank you.
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 10:54:58 2005



From: Ronald Anderson :      randerson20-at-tampabay.rr.com
Date: Fri, 03 Jun 2005 11:54:07 -0400
Subject: [Microscopy] Re: Photoshop 7 vs Photoshop CS2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank,

I'm using Adobe Creative Suite CS2 Premium to produce Microscopy Today.
The package includes Photoshop CS2. The first thing I did after
installing the suite was to install my Fovea Pro 3 filters, etc.
Everything works perfectly. What you see in Microscopy Today is
testament. The July issue, in the works, will be the first one done all
in CS2. There are some very valuable improvements in PS 7 (CS) and more
in PS 8 (CS2). Adobe is beginning to recognize the scientific market to
our considerable benefit.

I have no interest in Adobe other than being a very satisfied user.

Ron Anderson, Editor
Microscopy Today


frank.karl-at-degussa.com wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 12:23:26 2005



From: Joe Kulik :      juk12-at-psu.edu
Date: Fri, 3 Jun 2005 14:35:18 -0400
Subject: [Microscopy] TEM: Spare parts for Philips instrument

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm using Photoshop CS, which is Photoshop 8 (the number shown when it first
opens), with Fovea Pro 3 and Optipix 3 with no problems whatsoever. I don't
know the numerical designation for Photoshop CS2 but will learn more about
it tomorrow in a class.

Damian Neuberger, Ph.D.



We have a Philips EM420T on which one of the push-button function
selector switches (The M button for image mode) is starting to go bad.
(It sometimes does not engage smoothly and the OL current then changes.)

Replacement parts for these old instruments are depressingly expensive.

Does anyone have a decommissioned Philips TEM of the same vintage, and,
if so, would you be willing to give us (or sell us at a reasonable
price) spare parts like the aforementioned switch?

Or can anyone suggest a less expensive alternative than buying a $1500
switch from FEI?

Thanks to anyone with any suggestions!
_________________________________

Joseph Kulik
Research Associate
Materials Research Institute
The Pennsylvania State University
194 MRI Bldg
University Park, PA 16802

Telephone: 814-865-0344
Fax: 814-863-8561
e-mail: juk12-at-psu.edu
_________________________________




From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 15:32:00 2005



From: Ronald Anderson :      randerson20-at-tampabay.rr.com
Date: Fri, 03 Jun 2005 16:31:13 -0400
Subject: [Microscopy] Re: Re: Photoshop 7 vs Photoshop CS2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

David is right. Photoshop CS2 is PS 9, not PS 8 as I said in my previous
post. Check out Adobe site to see a list of new and improved features.

Ron Anderson


David Elliott wrote:

}
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Photoshop 8 came out with CS1. I am using it. I use lots of complex
} scripts and the Photoshop 8 scripting was worth my money.
} It has lots of other great things, but most of them are of no use to
} microscopists (unless you want to forge your data :-)
} David
}
}
} On Jun 3, 2005, at 8:30 AM, frank.karl-at-degussa.com wrote:
}
} }
} }
} } -----------------------------------------------------------------------
} } -------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------
} } --------
} }
} } I'm looking for some advice. I understand Adobe has issued
} } Photoshop 8 and
} } called it Photoshop CS2. I am currently using Photoshop 6 with
} } Reindeer
} } Graphics's Fovea Pro V3. Is anyone actually using Photoshop CS2 and
} } is it
} } better than Photoshop 7 and will my Povea Pro 3 work with it. I don't
} } want to be too far behind the curve as technology changes, but I
} } don't want
} } to be the public debuger either!
} }
} } Thanks!!
} }
} } Frank Karl
} } Degussa Corporation
} } Akron Technical Center
} } 3500 Embassy Parkway
} } Suite 100
} } Akron, Ohio 44333
} }
} }
} } 330-668-2235 Ext. 238
} }
} }
} } This e-mail transmission, and any documents, files or previous e-mail
} } messages attached to it may contain information that is confidential or
} } legally privileged. If you are not the intended recipient, or a person
} } responsible for delivering it to the intended recipient, you are hereby
} } notified that you must not read this transmission and that any
} } disclosure,
} } copying, printing, distribution or use of any of the information
} } contained
} } in or attached to this transmission is STRICTLY PROHIBITED. If you have
} } received this transmission in error, please immediately notify the
} } sender
} } by telephone or return e-mail and delete the original transmission
} } and its
} } attachments without reading or saving in any manner. Thank you.
} }
} }
}
}
}




From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 17:03:34 2005



From: Hong Yi :      hyi-at-emory.edu
Date: Fri, 3 Jun 2005 18:02:51 -0400
Subject: [Microscopy] (Microscopy) Pre-embedding Immunogold Labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All:

Could I ask those who do pre-embedding immunogold labeling routinely
to contact me off-line? I would like to discuss with you a few issues
regarding silver enhancement. Thank you in advance.

Hong
Emory SOM EM



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 3 22:26:45 2005



From: DrJohnRuss-at-aol.com
Date: Fri, 3 Jun 2005 23:25:56 EDT
Subject: [Microscopy] Re: Photoshop 7 vs Photoshop CS2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 6/3/05 11:14:03 AM, frank.karl-at-degussa.com writes:

} I'm looking for some advice. I understand Adobe has issued Photoshop 8
} and
} called it Photoshop CS2. I am currently using Photoshop 6 with Reindeer
} Graphics's Fovea Pro V3. Is anyone actually using Photoshop CS2 and is
} it
} better than Photoshop 7 and will my Povea Pro 3 work with it. I don't
} want to be too far behind the curve as technology changes, but I don't
} want
} to be the public debuger either!


CS2 (aka Photoshop 9) has quite a few new capabilities, but whether you need
them is hard to say. The handling of 16 bit images is quite a bit smoother and
more complete than in PS7, and the layers capability is also much better. The
Fovea Pro plugins work just fine with CS2, but you will have to re-record any
actions you may have set up to automate Photoshop as they do not translate
smoothly from PS9 to PS9.

John Russ


From MicroscopyL-request-at-ns.microscopy.com Sat Jun 4 08:19:44 2005



From: dgrimm-at-tulane.edu (by way of MicroscopyListserver)
Date: Sat, 4 Jun 2005 08:19:02 -0500
Subject: [Microscopy] viaWWW: Used Microscopes Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dgrimm-at-tulane.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, June 3, 2005 at 09:50:36
---------------------------------------------------------------------------

Email: dgrimm-at-tulane.edu
Name: Deborah Grimm

Organization: Tulane University

Title-Subject: [Microscopy] [Filtered] Used Microscopes

Question:

AMRAY 1700 SEM, has not been under vacuum for over 1 year, but should be operational, as-is where-is, any reasonable offer accepted.

JEOL JSM 820 SEM, analog, probably a parts machine. Any reasonable offer accepted.

We need to remove these microscopes from our lab by June 17th. Please e-mail me if you have any questions.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sat Jun 4 08:20:56 2005



From: DrJohnRuss-at-aol.com
Date: Sat, 4 Jun 2005 09:19:58 EDT
Subject: [Microscopy] correction for latest Microscopy Today

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The latest issue of Microscopy Today carries another of Jerry Sedgewick’s
articles about Photoshop techniques. Like every one of his others, it contains
much that is flat-out wrong or misleading, omits important information, and even
the parts that will actually work are by far not the best, easiest, or most
useful ways to accomplish the same purpose. I’ve tried in the past to send
corrective information (which has never gotten any response from either Sedgewick
or from Ron Anderson, who continues to accept these articles even though
several people besides me have complained about them and pointed out the numerous
errors). I suppose the need to fill up the magazine with unreviewed and
blatantly incorrect stuff outweighs any other criteria, which is unfortunate because
it reflects badly on other articles that are contributed by serious and
respectable scientists and doesn't help the magazine achieve respectability.

Anyway, to the subject: The article concerns methods for combining stereo
pair images to produce color anaglyphs. It is important to note some errors.
First, the drivel about converting 16 bit to 8 bit images is incorrect. You can
directly construct anaglyphs with either 8 or 16 bit images (or even a mixture,
which is admittedly unlikely), since Photoshop’s latest two versions (8 and 9,
also known as CS and CS2) handle 16 bit images just fine. And even if you do
want to convert a 16 bit image to 8 bits, the procedure described in the
article and shown in Figure 1 is wrong. That is the method (which was published
some time ago in this same magazine by Brent Neal) applies to converting a 12 bit
image to 16 bits, and has nothing whatever to do with the subject at hand. If
for some reason you do want to convert a 16 bit image to 8 bits, just select
Image-} Mode-} 8 bits per channel. Done.

But the main part of the article concerns putting together two images into
the color channels of a composite anaglyph. There is no reason whatever to use
the cumbersome procedure described, with extra black images and the merge
channels routine and awkward steps to align things. Just do the following (this
works with versions of Photoshop at least back to 6 - I haven’t tried older ones
but it probably works with 5 as well):
1) Select-} All and Edit-} Copy the left eye image to place it on the clipboard
2) Change the mode of the right eye image from Grayscale to RGB Color. This
will not change the appearance of the image on the screen, but if you look at
the Channels palette you will see that there are now three (RGB) channels all
containing the same data.
3) Click on the Red channel in the palette and paste in the image. The
Sedgewick article omits the important fact that the convention for viewing stereo
anaglyphs is to use the red channel for the left eye and either green or blue
for the right, and that is the way glasses are constructed. It’s difficult to
tell in the printed article, but it appears that the example shown in Figure 2
was put together backwards.
4) Click the eye symbol for RGB to display all of the channels. The original
right eye image is now shown in both green and blue, which works with either
the red/green or red/blue glasses and is brighter and easier to see than using
just one of those two channels.

You may be finished now, and can crop the image if desired and save it. But
if the alignment of the images is imperfect - either there is a vertical shift
between the images, or one is slightly rotated, or you just want to change the
horizontal displacement to alter the impression of depth - you can do it now
without any of the “take it apart, fiddle around, and try again†advice in
the Sedgewick article. The red channel is still selected from the steps above.
You can shift it (this is usually done more controllably with the arrow keys
than with the mouse - use the arrows to move the red channel a pixel at a time,
or depress the shift key as well to move it ten pixels at a time), or even
rotate it using the Edit-} Transform function. The key here is that you are
viewing the full color anaglyph through your glasses, because you turned on the RGB
visibility in step 4, and can therefore immediately see what your shifting of
the image does visually, which gives precise control. All of the stuff in the
Sedgewick article about locking or unlocking the layer, splitting and merging
the channels, creating an additional image, and so on, is just unnecessary and
confusing, and makes the task more difficult.

John Russ




From MicroscopyL-request-at-ns.microscopy.com Sat Jun 4 10:03:24 2005



From: km602223-at-comcast.net
Date: Sat, 04 Jun 2005 15:02:38 +0000
Subject: [Microscopy] Nikon Fluophot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone have an instruction manual for a Nikon Fluophot microscope?
Thank you,
Kathleen


From MicroscopyL-request-at-ns.microscopy.com Sat Jun 4 15:30:24 2005



From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Sat, 4 Jun 2005 16:29:42 -0400 (EDT)
Subject: [Microscopy] Re: Re: Photoshop 7 vs Photoshop CS2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is a demo version of CS2 that you can download and run. It has a
lot of fun new stuff, a lot definitely NOT useful for scientific imaging.
But two features for making figures that may be worth the ungrade are 1.)
the ability to shrink and reenlarge images without throwing out the
original pixels (I think this is from Illustrator) and 2.) a more sensible
font selection tool.

} Is anyone actually using Photoshop CS2 and is
} } it
} } better than Photoshop 7 and will my Povea Pro 3 work with it. I don't
} } want to be too far behind the curve as technology changes, but I don't
} } want
} } to be the public debuger either!

_________________________________________
Michael Cammer
Analytical Imaging Facility and
Dept. ASB Biophotonics Innovation Laboratory
Albert Einstein College of Medicine
1300 Morris Park Avenue, Bronx, NY 10461
718-430-2890 Fax 718-430-8996
work: http://www.aecom.yu.edu/aif/
personal: http://coxcammer.com/




From MicroscopyL-request-at-ns.microscopy.com Sat Jun 4 16:31:31 2005



From: Berns Buenaobra :      bbuenaobra-at-nip.upd.edu.ph
Date: Sun, 5 Jun 2005 05:32:39 -0700
Subject: [Microscopy] Device DDK

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:

I'm trying to customize a control application for a BX Olympus motorized
microscope but is short of a device driver kit for it (DDK). My problems
are determining the exact communications protocol to connect the serial
port to the controlling box.

Any one has the DDK or the protocol description?

Regards,

Berns B.



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 09:27:32 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 06 Jun 2005 10:26:22 -0400
Subject: [Microscopy] Re: Re: gaston naessens and somatid biology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Elaine et al:

Hoax/fraud/BS, pick one. Certainly not true.You might quote the
requestor an outrageous price and see how they respond. :-)

Geoff

} On Wed, 1 Jun 2005, Elaine Humphrey wrote:
}
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} }
} } Hello everyone
} } I have just had a request for the use of a somatoscope developed by
} } Gaston Naessens as they want colour at 30,000 times.
} }
} } I was wondering if someone was pulling my leg but when I did a google
} } search I got
} } http://www.sumeria.net/tech/naessens.html
} } and I am still wondering if someone has a huge hoax going. Has anyone
} } got experience with this type of microscope?
} }
} } Elaine
} }
} }
} } --
} } Dr. Elaine Humphrey
} } Director, BioImaging Facility
} } President, Microscopy Society of Canada (2003-2005)
} } University of British Columbia
} } 6270 University Blvd, mail-stop Botany
} } Vancouver, BC
} } CANADA, V6T 1Z4
} } Phone: 604-822-3354
} } FAX: 604-822-6089
} } e-mail: ech-at-interchange.ubc.ca
} } website: www.emlab.ubc.ca
} }
} }


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 10:31:19 2005



From: Skage Hem :      SHem-at-laurentian.ca
Date: Mon, 06 Jun 2005 11:29:58 -0400
Subject: [Microscopy] Sample charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello there,
First I wish to express my gratitude towards those list members who
helped me out with the electrical schematics and manuals for the
Cambridge S-120. Thanks alot!

Secondly, the samples seem to be charging/arching in a pulsating manner, even when the sample is thoroughly grounded. I know the column is somewhat dirty, do anyone know if this could be related to the trouble. If not do anyone have any ideas as what might be the cause.

Any advice is greatly appreciated.

Skage Hem





_______________________________________________________________

Skage Hem, Ph.D.
Research Scientist
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 11:03:32 2005



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 06 Jun 2005 11:00:14 -0500
Subject: [Microscopy] Re: Sample charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Are you certain the sample is grounded? We have some coated samples that it
seems we can never put enough gold on to eliminate charging. The samples
are insulating and rough. I can only examine small aggregates of particles
without encountering too much charging. I would try looking at a piece of
well-grounded, clean metal to be sure there is no chance of it being the
sample.

I went through that exercise with a dirty column a couple years ago. The
service engineer strongly suspected the sample, but when I loaded a bare
sample holder and still got drifting, he was finally convinced. He tore our
column down ALL the way and found some contamination deep within. I think
our scope was seven-years-old at the time. The location does not get dirty
so fast that it requires cleaning at every preventative maintenance, but we
will want to watch for problems and probably clean it at least every 4 or 5
years.

Warren

At 10:29 AM 06/06/05, you wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 11:08:47 2005



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Mon, 6 Jun 2005 11:51:04 -0400
Subject: [Microscopy] Sample charging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The samples seem to be charging/arching in a pulsating manner, even
when the sample is thoroughly grounded. I know the column is somewhat
dirty, do anyone know if this could be related to the trouble. If not
do anyone have any ideas as what might be the cause.
Skage Hem {SHem-at-laurentian.ca}
============
Skage,
Does your Cambridge S-120 have a spark plug or someplace where there
is a gap like a spark plug? [Stop laughing!]

I have a pulsing when a fleck of carbon gets built up inside the gap
of the spark plug in my Philips 200. It took weeks to find this
answer many years ago and it has occured only once in the last 10
years.
The cure is to run a piece of paper through the gap which is very
small to remove the carbon without needing to remove the spark plug
or readjust the gap space.

Pat Connelly psconnel-at-sas.upenn.edu
The University of Pennsylvania
Department of Biology
Philadelphia, PA 19104-6018
215-898-7145


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 11:21:44 2005



From: Ralph Common :      Ralph.Common-at-hc.msu.edu
Date: Mon, 6 Jun 2005 12:20:18 -0400
Subject: [Microscopy] Focus Extender 1.0

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am considering buying Reindeer Graphics Focus Extender 1.0, and would like to hear feedback from actual users. A search of the Web turned up little except copies of the original press release. I would like to know about ease of use, how well the program handles various types of subjects, and how it stacks up (pun intended) against similar programs. On a related topic, does anybody know of a source for a computer driven Z-drive that doesn't cost a fortune?

Ralph Common
Michigan State University
Division of Human Pathology



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 11:25:11 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 06 Jun 2005 09:24:24 -0700
Subject: [Microscopy] [MICROSCOPY] specimen heating up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I tried two other sets of specimens and they all heated up.
The ambient temperature is 70F. The heated temperature is
95F.

One specimen is a sputter coated (Pt) #1 cover slip stuck on
a pin stub via Nisshin EMS carbon tab. The slip is offset
slightly so I can ground it using Pella colloidal silver.

When I put this in the chamber, no beam, for about 10 minutes,
I pull it out and it is hot.

Then I did three Al film on silicon pieces on stubs which were attached using
Buehler wax but grounded using the Pella colloidal silver.
This large holder also got up to 95F in chamber vacuum without
any beam.

After some amount of time in air, the specimens seem to not
heat up. What is going on?

Strange. But it messes up image collection due to large
amount of drift.

gary g.



At 08:30 AM 5/31/2005, you wrote:
} Just a wild guess, but would the sample have heated up the same if mounted
} the same way and placed in a vacuum? I wonder if their might be something
} galvanic going on with all the metals involved. Maybe the beam had nothing
} to do with it.
}
} Warren
}
} At 04:49 PM 05/29/05, you wrote:
}
}
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 11:44:10 2005



From: Terry Mehr :      terry-at-AsylumResearch.com
Date: Mon, 6 Jun 2005 09:43:28 -0700
Subject: [Microscopy] AFM TechnicalSales Manager, UK Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Asylum Research is seeking a Technical Sales Executive to manage its
UK sales operations. The candidate should have experience both in
sales and technical applications for scanning probe/atomic force
microscopy (SPM/AFM) or scientific instrumentation/metrology tools.
Additional information on the position may be found at http://
www.AsylumResearch.com.

Terry Mehr
Director of Marketing
Asylum Research
www.AsylumResearch.com




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 11:54:28 2005



From: Ben McMorran :      mcmorran-at-physics.arizona.edu
Date: Mon, 6 Jun 2005 09:53:46 -0700
Subject: [Microscopy] Documentation for ISI WB6

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

We have scavenged the optics column from a defunct ISI WB6 electron microscope
(tungsten filament) in order to build an electron interferometer. Does anybody
out there have any technical specifications, engineering drawings, electrical
schematics, etc. for this equipment? We already have the user manual and an
adjustment manual.

Specifically, while we have been able to roughly focus and scan the beam using
our own power supplies, it would be really nice to know, for example, coil
currents that correspond to given focal lengths from a particular lens. Since
we're doing everything "by hand", we would love to know where all the beam
focal points are located. Dunno if that information is publicly available, but
I thought I'd ask. Thanks for your time!

--Ben McMorran

--
Ben McMorran
Research Assistant, Atom Optics Group
Department of Physics
University of Arizona
1118 E 4th St
Tucson, AZ
USA

ph. 520-621-2688



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 17:37:09 2005



From: Christine Weaver, McRI :      cweaver-at-mcri.org
Date: Mon, 6 Jun 2005 17:35:05 -0500
Subject: [Microscopy] Introduction to the Techniques of Forensic Soil Comparison Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

"Introduction to the Techniques of Forensic Soil Comparison"
Workshop Conducted by Skip Palenik
Inter/Micro 2005
McCrone Research Institute
July 14-15, 2005

This 2-day workshop, conducted by Skip Palenik, renowned expert in the field
of forensic microscopy and microanalysis, introduces innovative techniques
for the examination and interpretation of soil samples and soil comparison
evidence. Using a combination of several techniques (including visual and
microscopical methods, filtration, ultra-sonication, sieving, and chemical
treatments) soil samples can be separated into useful fractions. You will
learn and practice Skip's modified fractionation techniques to investigate
the most distinguishing particles that might ordinarily be overlooked,
present in low quantities, or hidden by clay and humic materials. This
workshop is unique in that it emphasizes a microscopical approach on the
level at which soil is most fundamentally distinct, i.e., on a
particle-by-particle basis. Forensic scientists from around the world have
verified that the use of these techniques on soil evidence has helped them
to identify the original source of an unknown sample in addition to
facilitating the process of comparison of known and unknown samples from
almost any geographical location.

The workshop will be conducted in the laboratories and classrooms of McCrone
Research Institute in Chicago, Illinois. The cost of the workshop is $200
which includes the use of equipment, teaching materials, lunch, snacks, and
refreshments. Please register early http://mcri.org/IM_03homep.html, class
size is limited.

Regards,
Christine

Christine Weaver
Inter/Micro Coordinator
McCrone Research Institute
2820 S. Michigan Avenue
Chicago IL 60616

312-842-7100
cweaver-at-mcri.org
http://www.mcri.org





From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 18:26:56 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 06 Jun 2005 16:24:13 -0700
Subject: [Microscopy] RE: [MICROSCOPY] specimen heating up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all:

Problem uncovered. Reason why is not totally clear.

The situation is that if the colloidal silver is applied
and then the specimen is put in the SEM after about two to
three hours, it gets hot. Leave the specimen in air or
out for a day, put in SEM, no heat. OK. So, what seems
to be happening is that the binders in the colloidal silver
product outgasses rapidly in a vacuum and accelerates an
otherwise slow and un-noticed heating when done in air over
a long period of time.

So, the solution is to either vacuum dessicate the stub
before putting in the SEM or wait over night before putting
into the SEM.

Any other explanations?

gary g.





At 09:54 AM 6/6/2005, you wrote:
} Well, it seems to me that you can't have a sample heating up that much
} without the stage getting hot too. And you'd need a fair bit of
} electrical current dissipating in resistive heat to cause it, must be
} several hundred mA (depending how much material is getting hot). Seems
} like you have a hot stage. Perhaps a dodgy motor on the stage drive, or
} heat transmitted from a diff pump (can't see how though). Or friction,
} does it depend how much you move the stage about? I guess the stage is
} warm to the touch?
}
} When you say:
}
} 'After some amount of time in air, the specimens seem to not
} heat up. What is going on?'
}
} Do you mean the specimen has been in air for a while, you put it back in,
} and it doesn't get hot - or do you mean when the chamber is vented up to
} air, the specimen cools down. (I guess the latter, cooling by convection
} currents in the air then.)
}
} So I guess I would look for electrical current going into the stage (eg
} pull the plug from the stage drive controller board, see if the effect
} goes away), friction, or something connected to the stage that is warm..
} Interesting one though, I'd be intrigued to know what it turns out to be.
}
} Good luck
}
} Richard
}
}
} ________________________________________
} Richard Beanland
} Analytical Services
} Bookham Inc
} Caswell
} Towcester
} Northants
} NN12 8EQ
} United Kingdom
} Tel. +44 1327 356362
} Fax. +44 1327 356775
} http://www.bookham.com
} ________________________________________
}
}
} -----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: 06 June 2005 17:24
} To: MSA listserver
} Subject: [Microscopy] [MICROSCOPY] specimen heating up
}
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 18:43:28 2005



From: Gordon Vrololjak :      gvrdolja-at-nature.berkeley.edu
Date: Mon, 6 Jun 2005 16:42:41 -0700 (PDT)
Subject: [Microscopy] Re: Nikon Fluophot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For manuals, contact Nikon directly (email/phone) and they will send you a
manual or a copy of one.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Sat, 4 Jun 2005, km602223-at-comcast.net wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
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} Does anyone have an instruction manual for a Nikon Fluophot microscope?
} Thank you,
} Kathleen
}


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 20:02:51 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 06 Jun 2005 17:58:57 -0700
Subject: [Microscopy] RE: [MICROSCOPY] specimen heating up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Who said that laws cannot be broken? Speeders do it
all the time. Red light runners...etc. Let's give
someone a ticket!

All I can say is that this is what I see. Try the experiment
for yourself and see what you get. perhaps there is a
variable in this situation that I am missing.

gary g.


At 06:14 PM 6/6/2005, you wrote:
} Huh? You've uncovered a violation of the second law of thermodynamics
} where the
} transition liq } gas, or adsorbed specie } gas is exothermic in spite of the
} increase in free energy????
}
} John
}
} Gary Gaugler wrote:
}
} }
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -------------------------------------------------------------------------------
} }
} } Hi all:
} }
} } Problem uncovered. Reason why is not totally clear.
} }
} } The situation is that if the colloidal silver is applied
} } and then the specimen is put in the SEM after about two to
} } three hours, it gets hot. Leave the specimen in air or
} } out for a day, put in SEM, no heat. OK. So, what seems
} } to be happening is that the binders in the colloidal silver
} } product outgasses rapidly in a vacuum and accelerates an
} } otherwise slow and un-noticed heating when done in air over
} } a long period of time.
} }
} } So, the solution is to either vacuum dessicate the stub
} } before putting in the SEM or wait over night before putting
} } into the SEM.
} }
} } Any other explanations?
} }
} } gary g.
} }
} } At 09:54 AM 6/6/2005, you wrote:
} } } Well, it seems to me that you can't have a sample heating up that much
} } } without the stage getting hot too. And you'd need a fair bit of
} } } electrical current dissipating in resistive heat to cause it, must be
} } } several hundred mA (depending how much material is getting hot). Seems
} } } like you have a hot stage. Perhaps a dodgy motor on the stage drive, or
} } } heat transmitted from a diff pump (can't see how though). Or friction,
} } } does it depend how much you move the stage about? I guess the stage is
} } } warm to the touch?
} } }
} } } When you say:
} } }
} } } 'After some amount of time in air, the specimens seem to not
} } } heat up. What is going on?'
} } }
} } } Do you mean the specimen has been in air for a while, you put it back in,
} } } and it doesn't get hot - or do you mean when the chamber is vented up to
} } } air, the specimen cools down. (I guess the latter, cooling by convection
} } } currents in the air then.)
} } }
} } } So I guess I would look for electrical current going into the stage (eg
} } } pull the plug from the stage drive controller board, see if the effect
} } } goes away), friction, or something connected to the stage that is warm..
} } } Interesting one though, I'd be intrigued to know what it turns out to be.
} } }
} } } Good luck
} } }
} } } Richard
} } }
} } }
} } } ________________________________________
} } } Richard Beanland
} } } Analytical Services
} } } Bookham Inc
} } } Caswell
} } } Towcester
} } } Northants
} } } NN12 8EQ
} } } United Kingdom
} } } Tel. +44 1327 356362
} } } Fax. +44 1327 356775
} } } http://www.bookham.com
} } } ________________________________________
} } }
} } }
} } } -----Original Message-----
} } } From: Gary Gaugler [mailto:gary-at-gaugler.com]
} } } Sent: 06 June 2005 17:24
} } } To: MSA listserver
} } } Subject: [Microscopy] [MICROSCOPY] specimen heating up
} } }
} } }
} } }
} } }
} } } -----------------------------------------------------------------------
} -------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} } } -----------------------------------------------------------------------
} --------
} } }
} } } I tried two other sets of specimens and they all heated up.
} } } The ambient temperature is 70F. The heated temperature is
} } } 95F.
} } }
} } } One specimen is a sputter coated (Pt) #1 cover slip stuck on
} } } a pin stub via Nisshin EMS carbon tab. The slip is offset
} } } slightly so I can ground it using Pella colloidal silver.
} } }
} } } When I put this in the chamber, no beam, for about 10 minutes,
} } } I pull it out and it is hot.
} } }
} } } Then I did three Al film on silicon pieces on stubs which were
} attached using
} } } Buehler wax but grounded using the Pella colloidal silver.
} } } This large holder also got up to 95F in chamber vacuum without
} } } any beam.
} } }
} } } After some amount of time in air, the specimens seem to not
} } } heat up. What is going on?
} } }
} } } Strange. But it messes up image collection due to large
} } } amount of drift.
} } }
} } } gary g.
} } }
} } }
} } }
} } } At 08:30 AM 5/31/2005, you wrote:
} } } } Just a wild guess, but would the sample have heated up the same if
} mounted
} } } } the same way and placed in a vacuum? I wonder if their might be
} something
} } } } galvanic going on with all the metals involved. Maybe the beam had
} nothing
} } } } to do with it.
} } } }
} } } } Warren
} } } }
} } } } At 04:49 PM 05/29/05, you wrote:
} } } }
} } } }
} } } } } --------------------------------------------------------------------
} ----
} } } ------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} } } } } --------------------------------------------------------------------
} ----
} } } -------
} } } } }
} } } } } Hi Listers:
} } } } }
} } } } } I've encountered a once only situation that I cannot
} } } } } explain. Perhaps someone has some ideas about what
} } } } } is going on.
} } } } }
} } } } } I put a specimen in the SEM for examination and when
} } } } } I pulled it out via the load lock, the specimen and
} } } } } holder were about 10 degrees hotter than ambient.
} } } } } My experience has been that whatever they go in at,
} } } } } they come out the same. This was simple SE imaging
} } } } } at 10KV with 30u High Current in Zeiss Supra 55VP
} } } } } in high vacuum mode. Specimen current was around 145pA.
} } } } }
} } } } } The specimen was a copper tube that had been plated
} } } } } with silver on the outside and inside. The piece was
} } } } } one half of the tube and about .5" long, attached to
} } } } } a pin stub using colloidal silver.
} } } } }
} } } } } EDS showed presence of K, O, Ag, Cu, and C. There were
} } } } } distinct areas of organic material that I take to be
} } } } } cyanide from the plating process. But how could SEM
} } } } } analysis of a specimen cause it to heat?
} } } } }
} } } } } Any ideas?
} } } } }
} } } } } gary g.
} } }
} } }
} } }
} } } =======================================================================
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} } } information contained in it may be confidential and/or protected by
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} } } =======================================================================



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 21:21:21 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 06 Jun 2005 18:31:56 -0700
Subject: [Microscopy] Re: RE: [MICROSCOPY] specimen heating up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I tried three pieces of Si coated with colloidal conductive
silver and began dessicating them in a Duniway chamber. This
got down to about 50mT using a Varian dual vane mech pump.

After thirty minutes, the Zeiss holder is not hot. So,
next is moving it into the E-6 Torr SEM chamber. More to
follow.

gary g.



At 05:58 PM 6/6/2005, you wrote:


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From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 22:29:04 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 06 Jun 2005 20:24:49 -0700
Subject: [Microscopy] Re: [MICROSCOPY] specimen heating up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, I think this problem is solved/isolated. The laws of
thermo are preserved. The SEM is a Zeiss Supra 55VP.
It has an Edwards XDS10 scroll pump and Varian TMP.
It is a dry system. Specimens are introduced via
a Fjeld load lock. All together, they are great.
The current problem seems to be the stage rotate motor.

It turns out that depending on some variables, the
Zeiss Supra 55VP stage rotate motor gets very hot.
This translates to a hot specimen holder and hot
specimens.

I see no way to avoid this other than to turn off R
until a new specimen needs it. This is not optimum.
But it seems to be the only solution. There were many
start up problems with this unit and the stage was
one of them. R was a big issue. I guess that it
has not gone away.

Thus, colloidal silver is not the culprit/cause.

gary g.


At 06:31 PM 6/6/2005, you wrote:


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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 6 23:09:30 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Mon, 6 Jun 2005 23:08:49 -0500
Subject: [Microscopy] Administrivia - correction for latest Microscopy Today

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} To: DrJohnRuss-at-aol.com
} Subject: [Microscopy] RE: correction for latest Microscopy Today

John

I have read with interest your recent Listserver posting of a few days ago. There is useful information in your text, specifically your methodology within Photoshop tools to construct stereo pairs. However, I note with concern that the first paragraph of your posting contains content which is in violation of the Microscopy Listserver rules.

This concern is not something to be taken lightly and I will quote from the Microscopy Listserver rules, which as subscribers EVERYONE receives a copy of (http://www.microscopy.com/Rules.html), and is a condition of their continued subscription. Specifically item #4 of those rules states:

----------------------------------------------------------------------
4.) This forum is not to be used as a platform to accuse or defame any individual or organization in any negative manner. You may disagree with any comment posted and post a reply, but this server may not be used to spread misleading, derogatory or disparaging comments under any conditions.
----------------------------------------------------------------------

In my opinion, you have crossed the line with your initial comments. It is fine to ask questions and/or offer alternatives or comments about any posting on the Listserver as that is certainly one of the purposes of this forum. However, in your posting you are making comments to the Listserver about an article which has no relation to the Listserver, the text for this technique never appeared in the Listserver or its archives, nor has anyone asked a question concerning this technique on the Listserver. Using this forum to disparage anyone is against our rules, and both Microscopy-Today and Jerry Sedgewick are no different than anyone else. I have not stood by idly when comparable situations happened in the past, nor will I do so in this situation.

I reiterate, posting information on your technique to create stereo pairs using Photoshop is fine as well as offering it as an alternative to methods of others. It is also fine that you disagree with Sedgewick (I disagree with alot of people and have disagreed with you on various issues over the years). Since you feel so strongly about this particular article in MT, I would encourage you to write a contributed article and submit it to MT about the methodoloy you just outlined, however, using the Listserver is an inappropriate forum to voice complaints or views about MT and/or Sedgewick.

If you (or anyone for that matter) feel that you are being ignored or have problems with MSA or one of its subsidiaries, please feel free to contact me off-line to discuss the situation and please send me a copy of any documentation you have submitted to the parties in question. As a former Council member I will be willing to bring your concerns to the attention of the appropriate individuals within MSA, or to the Council as appropriate.

I insist that EVERYONE using the Microscopy Listserver follows proper channels when they have problems with other organizations or individuals (MSA related or not) and no one is permitted to use the Listserver as a forum to disparage any individual or organization.


Nestor
Your Friendly Neighborhood SysOp
&
The Microscopy Listserver SysCop


-------------------------------------------------------------------------------
} From: DrJohnRuss-at-aol.com
} Date: Sat, 4 Jun 2005 09:19:58 EDT
} Subject: [Microscopy] correction for latest Microscopy Today
} To: microscopy-at-msa.microscopy.com


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
--------cut-----------




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 10:29:57 2005



From: Mike Zemyan :      mzemyan-at-schaferlabs.com
Date: Tue, 7 Jun 2005 08:29:08 -0700
Subject: [Microscopy] Mass Spectrometry Engineer / Technician Job Opening at Schafer Corporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mass Spectrometry Engineer / Technician Job Opening
at Schafer Corporation

Schafer Corporation is seeking an engineer / technician for its Mass
Spectrometry Group at its Vallecitos Laboratory near Sunol, California.
This position supports a multi-million dollar operation of thermal
ionization and secondary ion mass spectrometers and support instruments, and
interacts with a team of mass spectrometry scientists, engineers and
technicians. The candidate will also be expected to directly participate in
the analysis of samples and in the maintenance of auxiliary equipment.
Schafer Vallecitos Laboratory performs materials characterization and
related analytical services on commercial and government contracts. The
activities of the group include chemical analysis of materials on a
production basis, maintenance of several custom-built mass spectrometers,
development of new or improved mass spectrometric techniques, and quality
assurance of analytical data.

This position requires an individual who has proven technical abilities.
The ideal candidate must have a strong background in science or engineering,
or a related field of physical or chemical science, and experience operating
mass spectrometers or other analytical equipment in a laboratory
environment. The candidate must be able to operate independently with
little supervision. Other desired qualifications include:

- Expertise in data evaluation and quality control.
- Ability to write clear scientific and technical reports.
- Bachelor's degree in physical science or engineering.
- Minimum 4 years of laboratory experience.

Schafer offers a comprehensive array of benefits including dental, health
and life insurance, 4 weeks of vacation annually, and a 401k program. US
Citizenship required, as well as qualification for a DoD security clearance
- An Affirmative Action, EEO employer promoting diversity in the workplace.

Respond to job code VAL200502 - at jobs2-at-schaferlabs.com






From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 11:17:29 2005



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 7 Jun 2005 14:01:02 -0500
Subject: [Microscopy] high pass filter versus flat field correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Skage,
There are a few other possibilities that come to mind. First, you sample may
be grounded to the stub, but have you traced the ground all the way? Check
the sample holder, stage and the wire that goes to the feedthrough. My stage
grounding goes through a BNC plug, in case you want to test the specimen
current, and sometimes the plug needs cleaning because the oxide film builds
up and stops the grounding current. Remember the current is picoamps, so it
doesn't take much to stop it. I have had the grounding wire in the chamber
broken because it got caught in the o-ring when the chamber was pumped down.
The other thing to check is the scintillator. The aluminum coating on the
scintillator can become worn off by micro-discharges, until the bare plastic
button is showing, and this can build up a charge. A new scintillator button
is a good idea, or a new evaporated layer of Al and check that the button is
well grounded.
Of course dirt in the column or stage can cause these problems, as well.
----- Original Message -----
} From: "Skage Hem" {SHem-at-laurentian.ca}
To: {Microscopy-at-microscopy.com}
Sent: Monday, June 06, 2005 8:29 AM

I am working with digital TEM images that need flat field correction
and background subtraction to reduce fixed, uneven illumination and
noise (lint & electronic noise), respectively. I have a program that
came with the digital camera but it is cumbersome to use (at least,
so far).

In PhotoShop (version 6 and CS1) I can flatten the field using a high
pass filter but have been unable to use PS to subtract noise
(original image minus empty field). In PS, I tried using the
Image/Calculations feature as well as Image/Apply Image features. No
combination gave the desired effects.

Am I asking too much of PhotoShop, or simply not understanding how to
achieve this in PS? Maybe both, eh?

Any suggestions would be appreciated.

Thank you.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 14:10:51 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Tue, 07 Jun 2005 14:10:08 -0500
Subject: [Microscopy] Sectioning stainless steel wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is for the material Scientists among you,

We need to make 20-30µm sections of 200µm thick stainless steel wire coated
with a polymer. The object is to be able to do some chemical analysis on
the sections so as to visualize chemical distribution in the polymeric
coating. The sections will be imaged using Ramon technology.

We need suggestions as to embedding resin, type of microtome and type of
blades that may work for sections of this thickness and samples of this
type.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy





From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 14:30:04 2005



From: DrJohnRuss-at-aol.com
Date: Tue, 7 Jun 2005 15:29:15 EDT
Subject: [Microscopy] Re: high pass filter versus flat field correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There are three classic approaches to levelling background. Some are easier
to do in Photoshop than others.
1) If you can measure a background (ie. collect an image with no sample, or a
uniform sample present) then you can use layers modes to get the difference
between the original and the background. Unfortunately, depending on how your
camera works you may need to either subtract the background or divide by it (if
the camera is log or linear, respectively). Photoshop doesn't offer a ratio
layers mode (these are the same options as in the Calculations dialog), so you
would have to use a plugin (e.g., the Reindeer Fovea Pro suite which can
either subtract or divide.
2) If you can remove the features from the image (e.g. if they are skinny in
one dimension) to leave a background representation, then you can use that. In
Photoshop you can do a slightly crude but probably adequate job of a grey
scale morpholological closing or opening using the Filter-} Custom-} Maximum or
Minimum routine. For example if the features are dark on a bright background, you
would first use Maximum to remove the features and then Minimum with the same
radius to restore the scale of the background irregularities. This background
is then removed as in step 1.
3) In many cases, the gradually varying background from nonuniform
illumination can be modelled mathematically. In Fovea Pro, a cubic polynomial is fitted
to either the bright or dark parts of the image to construct a function for
removal. Sometimes you can accomplish the same thing with a large low-pass
filter (e.g., a Gaussian smooth). This blurs out the features to the point where
the image shows just the background, which you can then use as in step 1.

John Russ
=======

In a message dated 6/7/05 3:03:50 PM, bozzola-at-siu.edu writes:

} I am working with digital TEM images that need flat field correction
} and background subtraction to reduce fixed, uneven illumination and
} noise (lint & electronic noise), respectively. I have a program that
} came with the digital camera but it is cumbersome to use (at least,
} so far).
}
} In PhotoShop (version 6 and CS1) I can flatten the field using a high
} pass filter but have been unable to use PS to subtract noise
} (original image minus empty field). In PS, I tried using the
} Image/Calculations feature as well as Image/Apply Image features. No
} combination gave the desired effects.
}
} Am I asking too much of PhotoShop, or simply not understanding how to
} achieve this in PS? Maybe both, eh?
}
} Any suggestions would be appreciated.


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 14:44:34 2005



From: Roger Baker :      rcbaker-at-eden.infohwy.com
Date: Tue, 7 Jun 2005 14:43:42 -0500
Subject: [Microscopy] Re: Sectioning stainless steel wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I suspect that slicing through stainless steel wire without
disturbing its polymer coating on a microscopic level is going to be
a huge challenge.

The hard wire would probably mess up knives and if you try to grind a
flat on the end of the wire, the abrasive will get embedded in the
interface.

I would suggest simply slicing/stripping off the coating and looking
at that in cross section since its surface chemistry is not going to
change with the removal of the wire in contact.

-- Roger


On Jun 7, 2005, at 2:10 PM, Debby Sherman wrote:

}
}
} ----------------------------------------------------------------------
} --------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ---------
}
} This is for the material Scientists among you,
}
} We need to make 20-30µm sections of 200µm thick stainless steel
} wire coated
} with a polymer. The object is to be able to do some chemical
} analysis on
} the sections so as to visualize chemical distribution in the polymeric
} coating. The sections will be imaged using Ramon technology.
}
} We need suggestions as to embedding resin, type of microtome and
} type of
} blades that may work for sections of this thickness and samples of
} this
} type.
}
} Thanks,
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
}
}
}




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 15:59:40 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 7 Jun 2005 13:58:53 -0700
Subject: [Microscopy] Sectioning stainless steel wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debbie;
FIB.

John Mardinly
Intel


-----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: Tuesday, June 07, 2005 12:10 PM
To: message to: MSA list

This is for the material Scientists among you,

We need to make 20-30µm sections of 200µm thick stainless steel wire coated
with a polymer. The object is to be able to do some chemical analysis on
the sections so as to visualize chemical distribution in the polymeric
coating. The sections will be imaged using Ramon technology.

We need suggestions as to embedding resin, type of microtome and type of
blades that may work for sections of this thickness and samples of this
type.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy







From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 16:09:47 2005



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Tue, 07 Jun 2005 14:09:04 -0700
Subject: [Microscopy] RE: high pass filter versus flat field correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here's a favorite Photoshop recipe for background leveling digital TEM images (until someone suggests a better one). It uses Gaussian Blur as mentioned by J. Russ.

1. Open image or paste into new PS window.
2. Duplicate layer (twice)
3. Apply Gaussian Blur (50 pixels or your choice) to top layer
4. Invert image
5. Overlay image (blending mode option from Layers window)
6. Merge down

Repeat leveling operation 2 to 4 times until artifacts appear or contrast washes out.
Record as Action with different blur settings of choice.

For a more inclusive view of background leveling, see Russ tutorial at
reindeergraphics.com/tutorial/chap2/defect06.html

Larry Thomas
} ----------
} From: John J. Bozzola
} Sent: Tuesday, June 7, 2005 12:01 PM
} To: Microscopy-at-msa.microscopy.com
} Subject: [Microscopy] high pass filter versus flat field correction
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} I am working with digital TEM images that need flat field correction
} and background subtraction to reduce fixed, uneven illumination and
} noise (lint & electronic noise), respectively. I have a program that
} came with the digital camera but it is cumbersome to use (at least,
} so far).
}
} In PhotoShop (version 6 and CS1) I can flatten the field using a high
} pass filter but have been unable to use PS to subtract noise
} (original image minus empty field). In PS, I tried using the
} Image/Calculations feature as well as Image/Apply Image features. No
} combination gave the desired effects.
}
} Am I asking too much of PhotoShop, or simply not understanding how to
} achieve this in PS? Maybe both, eh?
}
} Any suggestions would be appreciated.
}
} Thank you.
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/~image/
} ##############################################################
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 16:12:32 2005



From: Bobby Hooghan :      hooghan-at-grandecom.net
Date: Tue, 7 Jun 2005 16:11:43 -0500
Subject: [Microscopy] Sectioning stainless steel wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debbie,
Second John's suggestion whole heartedly, you can lift out a small sample
with no trouble at all, let me know if you need help,
Bobby Hooghan,


-----Original Message-----
} From: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Tuesday, June 07, 2005 3:59 PM
To: Debby Sherman; message to: MSA listDebbie,

Debbie;
FIB.

John Mardinly
Intel


-----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: Tuesday, June 07, 2005 12:10 PM
To: message to: MSA list

This is for the material Scientists among you,

We need to make 20-30µm sections of 200µm thick stainless steel wire coated
with a polymer. The object is to be able to do some chemical analysis on
the sections so as to visualize chemical distribution in the polymeric
coating. The sections will be imaged using Ramon technology.

We need suggestions as to embedding resin, type of microtome and type of
blades that may work for sections of this thickness and samples of this
type.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy










From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 18:35:54 2005



From: Connie McManus :      conniemoss-at-relia.net
Date: Tue, 7 Jun 2005 17:35:07 -0600 (MDT)
Subject: [Microscopy] Re: RE: Sectioning stainless steel wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's no lie that FIB would be the best solution to this problem, but what
if you don't have a FIB?? It is actually easier to cut metals very thin
i.e. 20-80 nm. At this thickness, they can be examined in the EFTEM,
which will analyze and map the elemental composition at high resolution.


Bill McManus
Mt Ogden Scientific Services -Providing full service electron microscopy
for you
950 W Kershaw, Suite E
Ogden UT 84401
toll-free: 877/311-6677
direct: 801/745-2583
cell: 435/757/2975
fax: 435/514-1781
conniemoss-at-relia.net
www.mtogdensci.com


-------------------------------
Mardinly, John said:
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Debbie;
} FIB.
}
} John Mardinly
} Intel
}
}
} -----Original Message-----
} } From: Debby Sherman [mailto:dsherman-at-purdue.edu]
} Sent: Tuesday, June 07, 2005 12:10 PM
} To: message to: MSA list
} Subject: [Microscopy] Sectioning stainless steel wire
}
}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} This is for the material Scientists among you,
}
} We need to make 20-30µm sections of 200µm thick stainless steel wire
} coated
} with a polymer. The object is to be able to do some chemical analysis on
} the sections so as to visualize chemical distribution in the polymeric
} coating. The sections will be imaged using Ramon technology.
}
} We need suggestions as to embedding resin, type of microtome and type of
} blades that may work for sections of this thickness and samples of this
} type.
}
} Thanks,
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
}
} c
}
}
}




======


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 21:05:12 2005



From: sdunning-at-uga.edu (by way of Ask-A-Microscopist)
Date: Tue, 7 Jun 2005 21:04:31 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: how to calculate AFM resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sdunning-at-uga.edu) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, June 7, 2005 at 10:57:01
---------------------------------------------------------------------------

Email: sdunning-at-uga.edu
Name: Sarah Dunning

Organization: University of Georgia

Education: Graduate College

Location: Athens, Georgia

Question: I read in the literature that AFM's achieve "atomic resolution." How does one go about calculating this mathematically? Is it a matter of the tip size, geometry, tip-sample sensitivity, etc., all folded into one equation?



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 21:06:37 2005



From: przybylowicz-at-tlabs.ac.za (by way of MicroscopyListserver)
Date: Tue, 7 Jun 2005 21:05:54 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Search for second hand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (przybylowicz-at-tlabs.ac.za) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, June 6, 2005 at 12:44:43
---------------------------------------------------------------------------

Email: przybylowicz-at-tlabs.ac.za
Name: Wojciech Przybylowicz

Organization: Materials Research Group, iThemba LABS

Title-Subject: [Microscopy] [Filtered] Search for the second hand automatic refilling system

Question: Our laboratory is looking for the automatic refilling device (7019-11006) that operates with Reichert-Jung FC4 Ultracut Cryosystem. It is composed of the control module (6527-00001), dewar with solenoid valve (7019-31030) and heating element inside, and cooling chamber (7019-1150). If you know of any laboratory willing to offer it to us for free or for a reasonable price, please let me know.

Wojciech Przybylowicz

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 7 21:07:20 2005



From: kristinat-at-hotmail.com (by way of MicroscopyListserver)
Date: Tue, 7 Jun 2005 21:06:36 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: UV filter for a 96-well

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kristinat-at-hotmail.com) from http://www.microscopy.com/MLFormMail.html on Tuesday, June 7, 2005 at 11:09:35
---------------------------------------------------------------------------

Email: kristinat-at-hotmail.com
Name: Kristina Turner

Organization: New Mexico State University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I am looking for a 620 or 625 nm UV filter for a 96-well plate reader. I will get the details of model number, etc. of the plate reader it would need to fit. I am doing Minimal Inhibitory Concentration susceptibility testing on Staphylococcus aureus with gossypol for my Master's thesis research project. The Professor in the Biology department that has a 96-well plate reader that I could use to read my results does not have a 625 nm UV filter that I need.
His plate reader is 10 years old, so it is pretty old.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 00:43:47 2005



From: Gordon Couger :      gcc-at-couger.com
Date: Wed, 08 Jun 2005 00:43:03 -0500
Subject: [Microscopy] Re: Re: high pass filter versus flat field correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ImageJ http://rsb.info.nih.gov/ij/ is bit harder to use but will
do perfectly remove a background image from form an image by
taking a blank back ground image an using the
Process} math} difference to remove the blank frame from the
image. I leaves an image that need to be put on a flat back
ground but I haven't got a round toit yet.

ImageJ is a free tool developed by NIH and run on almost all
platforms. It has a large development community writing plug ins
and background is mentioned in several pulgins.

Gordon Couger
Stillwater, OK
www.couger.com/gcouger


} In a message dated 6/7/05 3:03:50 PM, bozzola-at-siu.edu writes:
}
}
} } I am working with digital TEM images that need flat field
correction
} } and background subtraction to reduce fixed, uneven
illumination and
} } noise (lint & electronic noise), respectively. I have a
program that
} } came with the digital camera but it is cumbersome to use (at
least,
} } so far).
} }
} } In PhotoShop (version 6 and CS1) I can flatten the field
using a high
} } pass filter but have been unable to use PS to subtract noise
} } (original image minus empty field). In PS, I tried using the
} } Image/Calculations feature as well as Image/Apply Image
features. No
} } combination gave the desired effects.
} }
} } Am I asking too much of PhotoShop, or simply not
understanding how to
} } achieve this in PS? Maybe both, eh?
} }
} } Any suggestions would be appreciated.
}
}
}




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 04:09:50 2005



From: Richard Beanland :      richard.beanland-at-bookham.com
Date: Wed, 8 Jun 2005 10:08:24 +0100
Subject: [Microscopy] Sectioning stainless steel wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would have concerns about surface contamination from FIB which could affect the results, not to mention changes in the polymer itself from damage to the ion beam.
This isn't too different from the optoelectronic packages we section on occasion, which can have everything from stainless steel through glass to adhesives. It's not clear to me why you need a thin section for Raman - can't you just pot the wire in an appropriate resin (we use Struers but I'm sure other suppliers are just as good) and grind/polish as for a standard metallographic section? When there are changes in material hardness it can be difficult to keep the surface flat, but a little experimentation for your application with different pads should get you there. This should give you a nice clean and flat surface for analysis, and a few polishing pads and a bit of resin is slightly cheaper than a FIB.

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


-----Original Message-----
} From: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: 07 June 2005 20:10
To: message to: MSA list

This is for the material Scientists among you,

We need to make 20-30µm sections of 200µm thick stainless steel wire coated
with a polymer. The object is to be able to do some chemical analysis on
the sections so as to visualize chemical distribution in the polymeric
coating. The sections will be imaged using Ramon technology.

We need suggestions as to embedding resin, type of microtome and type of
blades that may work for sections of this thickness and samples of this
type.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy





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From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 06:26:11 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Wed, 8 Jun 2005 08:55:29 -0230
Subject: [Microscopy] ESEM: accelerating oxidation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is anyone aware of a method for accelerating oxidation while observing with
ESEM?

tia :o)
michael shaffer
(709) 737-8346 (voice)
(709) 737-2589 (FAX)
{www.micro-investigations.com}
{www.esd.mun.ca/epma/}
Alexander Murray Bldg, Rm ER4063
Memorial University of Newfoundland
St. John's, NL
Canada, A1B 3X5



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 06:31:53 2005



From: microbill-at-mohawk.net
Date: Wed, 8 Jun 2005 07:31:10 -0400
Subject: [Microscopy] Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



HIROX-USA, INC.
}
} Hirox USA, distributor of Industrial Video-Microscope systems, is seekng a
} Sales Engineer who has prior experience with the sale of capital equipment.
} The job requirements include: Demonstration of the instruments, travel and
} the ability to lift equipment up to 100LB.
} The position is for the New Jersey area. This industry is growing
} rapidly and our customers include many Fortune 500 companies. see
} {http://www.hirox-usa.com} www.hirox-usa.com Email to:
} {mailto:mail-at-hirox-usa.com} mail-at-hirox-usa.com or Fax resume to 201.342.7322


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 11:53:03 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Wed, 8 Jun 2005 09:52:18 -0700 (PDT)
Subject: [Microscopy] Any SEM/EDS labs in Shanghai, China?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just got a request from someone in China if there
are any labs available in China that can do SEM/EDS
work. Can anyone provide contact information?

Stu Smalinskas, P.E.
Metallurgist
SKF North American Technical Center
Plymouth, Michigan
(734) 414-6862
stu.smalinskas-at-skf.com



__________________________________
Discover Yahoo!
Have fun online with music videos, cool games, IM and more. Check it out!
http://discover.yahoo.com/online.html


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 13:34:07 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Wed, 8 Jun 2005 11:32:52 -0700 (PDT)
Subject: [Microscopy] Re: Any SEM/EDS labs in Shanghai, China?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To add, this request was from a manufacturer of
automotive mirrors. They need EDS work on the layers
of the mirrored surface. I’ve been doing this work
for their customer, but they want to have it done
locally for themselves.

Stu

--- Changhui LEI {clei-at-uiuc.edu} wrote:

} Contact Shanghai Jiaotong U, Fudan U.
}
} They should have good TEM, SEM, lab.
}
} Or you can contact SMICS. They have SEM/EDS. But I
} do not know if they like to check metals for you as
} SMICS is a semiconductor manufacture.
}
} Ch
}
} ---- Original message ----
} } Date: Wed, 8 Jun 2005 09:52:18 -0700 (PDT)
} } From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
} } Subject: [Microscopy] Any SEM/EDS labs in Shanghai,
} China?
} }
} }
} } I just got a request from someone in China if there
} } are any labs available in China that can do SEM/EDS
} } work. Can anyone provide contact information?
} }
} } Stu Smalinskas, P.E.
} } Metallurgist
} } SKF North American Technical Center
} } Plymouth, Michigan
} } (734) 414-6862
} } stu.smalinskas-at-skf.com




__________________________________
Discover Yahoo!
Find restaurants, movies, travel and more fun for the weekend. Check it out!
http://discover.yahoo.com/weekend.html



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 13:37:50 2005



From: Scott Griffith :      skod-at-ises-llc.com
Date: Wed, 08 Jun 2005 12:37:07 -0600
Subject: [Microscopy] Wanted to buy: used/serviceable stepper stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I need a working/repairable scanning stage, ideally already set up for
Leitz Orthoplan or Ergolux-compatible mounting dimensions. I've
essentially abandoned trying to get my Ergolux's servo-driven stage
working again due to the unavailability of parts and diagnostic info. The
eventual goal is to drive the unit with an Oasis-4i to create an automated
image capture system. If you have applicable stage hardware available for
a reasonable price, please contact me. Thanks in advance.

--
Scott Griffith
ISES-LLC
9745 Steeplechase Drive
Franktown, CO 80116
303-660-2541 voice
303-660-2542 fax
skod-at-ises-llc.com


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 15:06:32 2005



From: Gib Ahlstrand :      ahlst007-at-umn.edu
Date: Wed, 08 Jun 2005 15:05:18 -0500
Subject: [Microscopy] Diffusion pump oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

I'm trying to characterize two kinds of diffusion pump oils that I found in
my "back room":

1. Balzers-Oil 71 für Diffusionspumpen. Ch 76-946


2. Trafo Olie, Dialac C #132250466801)


They both look like they have been around for some years. Does anyone know
what kind of oils they are, eg. Silicone based or not?

My TEM and SEM have dedicated diff pump oils supplied by the manufacturer,
but I'm wondering if either of the ones mentioned above would be OK to use
in the diff pump of my Denton vacuum evaporator, which I use for routine
carbon or gold-palladium coatings.

Thanks in advance!

Gib
--
Gib Ahlstrand, Scientist
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-2785 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/





From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 16:16:27 2005



From: Elaine Humphrey :      ech-at-interchange.ubc.ca
Date: Wed, 08 Jun 2005 14:58:57 -0700
Subject: [Microscopy] Re: hydrogel mesh

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The second position (Trafo Olie, Dialac C) is a transformer oil- don't use
it in vac. pumps. It is cheap - under $4 per gallon. If it is old and
discolored- dispose of it. The first one (Balzers-Oil 71) must be OK for
diff. pump use but:

a) is it in good condition (clean? clear? not oxydized/contaminated)?

b) you must completely clean traces of previously used oil from diff pump
(these oils don't perform well if at all when mixed), unless you know for
sure that diff pump was previously filled with the same type oil;

c) unless mistery oil is Santovac or Pentavac (very viscous at room
temperature, like honey, clear, either colorless or has very light yellow
color), it is cheap and IMO isn't worth the effort of trying it with
uncertain results. But I would definitely try old Santovac, especially for a
large diffusion pump, since it costs $1 and more per cc. Otherwise don't
bother.

Vitaly Feihgold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
} From: "Gib Ahlstrand" {ahlst007-at-umn.edu}
To: "Microscopy Listserver" {Microscopy-at-MSA.Microscopy.com}
Sent: Wednesday, June 08, 2005 4:05 PM

Hi Pat
I agree with Debby. We have one too but I think your nearest cryo sem
is University of New Brunswick, Frederickton.
Elaine

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada (2003-2005)
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 17:05:13 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 08 Jun 2005 15:04:48 -0700
Subject: [Microscopy] Re: Re: Diffusion pump oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Vitaly right
I would not put unknown quality diff oil into my pump: if it went wrong,
you will spend days to clean pump up. Personally, I am using Santovac -
it's serving my DV502A for 11 years without any problems: vacuum 5x10-7
torr after a few hours, no LN2. Sergey

At 01:58 PM 6/8/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu






From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 20:14:48 2005



From: acassell-at-mail.arc.nasa.gov (by way of MicroscopyListserver)
Date: Wed, 8 Jun 2005 20:14:05 -0500
Subject: [Microscopy] viaWWW: video capture card for Gatan Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (acassell-at-mail.arc.nasa.gov) from
http://www.microscopy.com/MLFormMail.html on Wednesday, June 8, 2005
at 19:04:33
---------------------------------------------------------------------------

Email: acassell-at-mail.arc.nasa.gov
Name: Alan Cassell

Organization: NASA Ames Research Center

Title-Subject: [Microscopy] [Filtered] MListserver: Gatan video capture card?

Question: We have a bottom mount Gatan fiber optic coupled camera on
our JEOL2000FX TEM and we would like to use a video capture card to
grab frames from the monitor for the camera. Has anyone tried
regular commercial video cards? And if so, which ones work the best?

Thanks,

Alan

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 8 20:45:51 2005



From: Anthony Garratt-Reed :      tonygr-at-MIT.EDU
Date: Wed, 08 Jun 2005 21:41:36 -0400
Subject: [Microscopy] Re: viaWWW: video capture card for Gatan Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've done it, and it certainly worked first time, no problems. I used old
ISA cards, (well - they weren't old when I used them!!) I think an M-vision
MV100 and a Synoptics card - possibly a Syntillate, but I'm not sure about
that. Of course you would use newer ones, but I would be quite confident
that any one with a standard TV input would work fine. It would all depend
on the software that came with them - the "demo" software will allow you to
capture and save images, but may not be as nice as you would like. You
probably wouldn't want to get into writing your own software, and 3rd.
party software can be expensive.

Tony.

At 09:14 PM 6/8/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*********************************************
Anthony J. Garratt-Reed, M.A., D.Phil
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
*********************************************
Phone: (617) 253-4622
Fax: (617) 258-6479
*********************************************



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 9 07:16:40 2005



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Thu, 9 Jun 2005 14:11:54 +0200
Subject: [Microscopy] Re: Diffusion pump oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gib

In an 1997 catalog from Pfeifer/Balzers I have the following indications :

Synthetique oil 71A :

vapor pressor 2.10-8 mbar at 20°C
good chemical resistance
Good heat and oxydation resistance
Optimale pressure domaine 10E-3 to 5E-7 mbar
Ultimate pressure : {6E-9 with LN2 trap
{4E-7 with water trap
{1E-6 with air trap (what is an air trap ????)

Recommended for metalurgie, electronic tube manufacturing, E-beam
welding, sputtering, thermal evaprators, etc. Not for particules
accelerators.

So no problem for your evaporator, but it seems to be a rough cheap oil,
and not a Santovac quality one. A bit less good than DC 704.

If you send me your fax number, I can fax you the catalog sheet, but it is
in french. It gives the specifications from 61A, 71A, DC704, AN175 and
Santovac in regard.


J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matériaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr

On Wed, 8 Jun 2005, Gib Ahlstrand wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi Listers,
}
} I'm trying to characterize two kinds of diffusion pump oils that I found in
} my "back room":
}
} 1. Balzers-Oil 71 für Diffusionspumpen. Ch 76-946
}
}
} 2. Trafo Olie, Dialac C #132250466801)
}
}
} They both look like they have been around for some years. Does anyone know
} what kind of oils they are, eg. Silicone based or not?
}
} My TEM and SEM have dedicated diff pump oils supplied by the manufacturer,
} but I'm wondering if either of the ones mentioned above would be OK to use
} in the diff pump of my Denton vacuum evaporator, which I use for routine
} carbon or gold-palladium coatings.
}
} Thanks in advance!
}
} Gib
} --
} Gib Ahlstrand, Scientist
} Electron Optical Facility, University of Minnesota, CBS Imaging Center,
} 35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
} (612)624-2785 FAX, ahlst007-at-tc.umn.edu
} http://www.cbs.umn.edu/ic/
}
}
}
}




From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 08:28:26 2005



From: Stacey.Andringa-at-uc.edu (by way of MicroscopyListserver)
Date: Fri, 10 Jun 2005 08:27:42 -0500
Subject: [Microscopy] viaWWW: labeling with HRP-gold and SPA-gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Stacey.Andringa-at-uc.edu) from
http://www.microscopy.com/MLFormMail.html on Friday, June 10, 2005 at
08:25:18
---------------------------------------------------------------------------

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I have typeII cells that I want to label with HRP-gold and
SPA-gold. Someone else has made the labels for me. I have a protocol
to label the cells, but I want to make cytospin slides and check them
to see if the cells are really labelled. I have a Vector kit that
uses DAB (SK-4100).
How can I block endogenous proteins?
Thanks.

Stacey Andringa

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 12:58:47 2005



From: Brian Matsumoto :      matsumot-at-lifesci.ucsb.edu
Date: Fri, 10 Jun 2005 11:01:23 -0700
Subject: [Microscopy] Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Department of Molecular, Cellular, and Developmental Biology and the
Neuroscience Research Institute are sponsoring an advance course on light
microscopy. This 5-day workshop will be offered from September 5 through
September 9, 2005 and will consist of lectures and laboratory exercises that
will run from 9 am to approximately 5 pm each day. The seminar/workshop will
be intensive, enabling participants to develop theoretical and hands-on
expertise with light microscopes. Attendees will interact closely with the
instructors while using modern research grade microscopes, cameras, and
computers. The seminars and laboratories will cover basic optical theory and
how it pertains to increasing contrast (signal to noise ratio) in biological
samples. Fundamental techniques such as fluorescence, phase contrast,
Nomarski Differential Interference Contrast, and darkfield imaging will be
taught and attendees will use microscopes equipped with these optical
enhancement accessories. In addition, the theory and practice of electronic
image acquisition (analog and digital) will be presented and attendees will
work with low-light cameras, digital image processing computers, and
morphometric programs. There are five research grade microscopes, five
electronic imaging cameras, two computer workstations, and one confocal
microscope. With a maximum enrollment of 10 students, there will be ample
opportunity to work with all of the microscopes and cameras. For those so
interested, intensive hands-on instruction and guidance on the confocal
microscope will be provided.

For a fuller description of the workshop please check the web address below.
Enrollment forms can be completed online and this workshop provides an
opportunity to have a working-vacation in Santa Barbara, California.

http://www.lifesci.ucsb.edu/mcdb/events/imaging_workshop/index.html

Brian Matsumoto, Ph.D.
Neuroscience Research Institute and
Department of Biology: MCDB
University of California
Santa Barbara, CA 93106-9610

Phone: 805-893-8702
FAX: 805-893-4724




From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 13:42:11 2005



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Fri, 10 Jun 2005 14:24:54 -0400
Subject: [Microscopy] Re: Kodak EM4489 films sensitivity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

6/1/04, Tindall, Randy D. wrote:
} ------------------------------------------------------------------------------
} Has as anybody noticed a second round of changes in this film? ...
}
} This leads me to think that there's a possibility that this film was
} reformulated yet again at some point, since nothing obvious changed in
} our procedures. Any similar experiences out there?
Randy,
I was justs cleaning out a lot of email messages from my file and
re-read your much longer message.
Did you ever find out if the 4489 was changed a second time?

I still have 3 boxes of 2004-06 which from my calculation should be
"original film"
but the other stock I have is 2005-06 which would fall into the time
frame that your
film batch might have been when you wrote the message. Does this make sense?

Storm just hit Bye-
Pat


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 14:03:19 2005



From: Webster, Paul :      PWebster-at-hei.org
Date: Fri, 10 Jun 2005 11:59:50 -0700
Subject: [Microscopy] Re: Re: Kodak EM4489 films sensitivity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Randy,

I didn't see your original message so my reply is late. Yes, I have noticed
a difference in the "new" 4489 film since it was changed. We have had to
reset out TEM to accommodate for the much darker images that suddenly
started to appear.

It seems like they are working at getting everyone to convert to digital in
a very covert way.

Paul.


On 6/10/05 11:24 AM, "Pat Connelly" {psconnel-at-sas.upenn.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} 6/1/04, Tindall, Randy D. wrote:
} }
-----------------------------------------------------------------------------} }
-
} } Has as anybody noticed a second round of changes in this film? ...
} }
} } This leads me to think that there's a possibility that this film was
} } reformulated yet again at some point, since nothing obvious changed in
} } our procedures. Any similar experiences out there?
} Randy,
} I was justs cleaning out a lot of email messages from my file and
} re-read your much longer message.
} Did you ever find out if the 4489 was changed a second time?
}
} I still have 3 boxes of 2004-06 which from my calculation should be
} "original film"
} but the other stock I have is 2005-06 which would fall into the time
} frame that your
} film batch might have been when you wrote the message. Does this make sense?
}
} Storm just hit Bye-
} Pat
}



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 14:59:10 2005



From: Marc Pypaert :      marc.pypaert-at-yale.edu
Date: Fri, 10 Jun 2005 15:58:27 -0400
Subject: [Microscopy] Re: Re: Re: Kodak EM4489 films sensitivity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

That explains a lot!
We've been having darker negatives later too,
but I had assumed that it was either a change in
temperature or developer dilution that had caused
it.

Marc

On Friday, June 10, 2005, at 02:59 PM, Webster, Paul wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} Dear Randy,
}
} I didn't see your original message so my reply is late. Yes, I have
} noticed
} a difference in the "new" 4489 film since it was changed. We have had
} to
} reset out TEM to accommodate for the much darker images that suddenly
} started to appear.
}
} It seems like they are working at getting everyone to convert to
} digital in
} a very covert way.
}
} Paul.
}
}
} On 6/10/05 11:24 AM, "Pat Connelly" {psconnel-at-sas.upenn.edu} wrote:
}
} }
} }
} } ----------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------
} -------}
} -
} }
} } 6/1/04, Tindall, Randy D. wrote:
} } }
} -----------------------------------------------------------------------
} ------} }
} -
} } } Has as anybody noticed a second round of changes in this film? ...
} } }
} } } This leads me to think that there's a possibility that this film was
} } } reformulated yet again at some point, since nothing obvious changed
} } } in
} } } our procedures. Any similar experiences out there?
} } Randy,
} } I was justs cleaning out a lot of email messages from my file and
} } re-read your much longer message.
} } Did you ever find out if the 4489 was changed a second time?
} }
} } I still have 3 boxes of 2004-06 which from my calculation should be
} } "original film"
} } but the other stock I have is 2005-06 which would fall into the time
} } frame that your
} } film batch might have been when you wrote the message. Does this make
} } sense?
} }
} } Storm just hit Bye-
} } Pat
} }
}
}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 15:37:13 2005



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 10 Jun 2005 13:22:54 -0700
Subject: [Microscopy] [MICROSCOPY] Re: specimen heating up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to the help from David Frey of Zeiss NY,
the problem is software related. Versions of the
SmartSEM prior to 5.00.08 had the problem. Field
service here has 09. So this should fix the problem.
If you have the heating problem, contact your service
folks for a newer software version.

PS: disabling stage R does not remove drive from the
motor. It just denies R commands.

gary g.


At 08:24 PM 6/6/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 16:22:33 2005



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Fri, 10 Jun 2005 16:21:48 -0500 (CDT)
Subject: [Microscopy] Re: Re: Re: Re: Kodak EM4489 films sensitivity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

We've been having the same problem. Kodak has also changed the
instructions on how to mix D-19 developer, though, and that may be
compounding the problem. The old (paper envelopes) instructed adding the
package to 3.8 liters of water, while the newer (plastic) envelopes have
you start with 3 liters, dissolve the solution, then bring the final
volume to 3.8 liters. Unfortunately, I threw out my last paper envelope a
few weeks before mixing a batch with the stuff in the plastic envelope so
I'm not sure whether the weight of the old (???) and new packages (551
grams) are the same or not.

Does anyone know how much powder is in a 1 gallon (3.8 liter) size paper
envelope?

Heather Owen


Dr. Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
Lapham Hall, P.O. Box 413
Milwaukee, WI 53210
USA

Phone: (414)229-6816



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 16:27:45 2005



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Fri, 10 Jun 2005 16:27:03 -0500 (CDT)
Subject: [Microscopy] Film Sensitivity - error in previous posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Oops. I had an empty Dektol envelope instead of an empty D-19 envelope.
The actual weight of the plastic 3.8 liter size package is 607 grams, not
551 grams.

Friday afternoon brain slippage.

Heather Owen


Dr. Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
Lapham Hall, P.O. Box 413
Milwaukee, WI 53210
USA

Phone: (414)229-6816



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 16:50:20 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Fri, 10 Jun 2005 16:49:36 -0500
Subject: [Microscopy] Re: Re: Re: Re: Re: Kodak EM4489 films sensitivity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

heather, marc, etc

having some D-19 of older stock (just getting into the next order), the
package size with the paper bag is 12gms less than for the new plastic
bags. this difference could be just packaging. oh, yeah, that is for
D-19, not dektol. our friday afternoon brain seizure has not yet hit up
her in the frozen north....

as far as technique, the original procedure in my books - like 30+ years
ago - was make it up in 3L at 37-50C, let cool, and then bring up to
3.8L volume. that is the way i've always done it wherever i have gone.

hope that trivia helps.

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Fri Jun 10 16:56:29 2005



From: Greg Strout :      gstrout-at-ou.edu
Date: Fri, 10 Jun 2005 16:55:45 -0500
Subject: [Microscopy] Re: Re: Re: Re: Re: Kodak EM4489 films sensitivity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Heather,
I don't know about the paper bags as we don't have any of those, but we
do have the cans that pre-date the paper bags and they have 595 grams
with the instructions to add powder to 1 U.S. gallon (3.8 liters) at
100F (38C).
Greg

Heather A Owen wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From MicroscopyL-request-at-ns.microscopy.com Sat Jun 11 22:14:04 2005



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 11 Jun 2005 23:13:23 -0500
Subject: [Microscopy] SEM/EDS service labs in China

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Stu Smalinskas wrote:
==============================================
I just got a request from someone in China if there
are any labs available in China that can do SEM/EDS
work. Can anyone provide contact information?
==============================================
See URL
http://www.2spi.com/agntdist/china.html

The first firm listed is run by Dr. Susan Tai, formerly an executive with
FEI Far East. She knows everyone who does this kind of work in China.

The third firm, KYKY, is a Chinese manufacturer of SEMs and at least at one
time would run commercial samples for clients in their demo lab in Beijing.
I have seen their newest model operate and it produces quite a good image.
They are physically located next door to the large and very well equipped
lab of the Academia Sinica (Chinese Academy of Sciences) so if they could
not do what was needed, I am sure the lab next door could.



Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================




From MicroscopyL-request-at-ns.microscopy.com Sat Jun 11 23:07:13 2005



From: Ellery Frahm :      frah0010-at-tc.umn.edu
Date: Sat, 11 Jun 2005 23:06:31 -0500
Subject: [Microscopy] lab noise control and acoustic panels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Microscopy folks,

Our electron microprobe laboratory has a chiller and sizable air
conditioner, and thanks to Facilities Management at our university,
there's little chance of getting them moved out of the lab. Since I
cannot move the noise sources out of the lab, I've been thinking
about installing acoustic panels to help with noise control. I've
seen this done at a few other electron microscope labs, and the staff
seemed happy with the results. I've been looking online at acoustic
panels, and there are various materials, shapes, and designs out
there. Has anyone else done this? What materials and shape did you
use? Are you happy with the results? Any problems (shedding of
fibers or dust, etc)? Is there anything you wish you'd done
differently? Any suggestions or opinions are welcome.

Thanks in advance,

Ellery

--------------------
Ellery E. Frahm
Research Fellow & Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu/



From MicroscopyL-request-at-ns.microscopy.com Sun Jun 12 11:08:16 2005



From: Larry Allard :      allardlfjr-at-ornl.gov
Date: Sun, 12 Jun 2005 12:07:01 -0400
Subject: [Microscopy] Re: lab noise control and acoustic panels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ellery:

I personally would avoid the use of foam-based materials such as
Sonex, as they degrade with time (~10years) and become a real mess to
clean up and replace. We have begun using absorber/barrier blanket
material, such as provided by Netwell Noise Control (and others),
which does a great job and looks like it will last virtually forever.
Get on Google and check it out. You can have special enclosures made
to your own design, to isolate pumps and chillers, for example.
Larry





} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Dr. Lawrence F. Allard
Distinguished Research Staff Member
High Temperature Materials Laboratory
Microscopy, Microanalysis, Microstructures Group
Metals and Ceramics Division
Oak Ridge National Laboratory
1 Bethel Valley Road
PO Box 2008
Oak Ridge, TN 37831-6064
(note: the last 4 lines are sufficient for mailing or overnight
courier service)
865-574-4981
865-576-5413 Fax
http://www.ms.ornl.gov/htmlhome/mauc




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 13 06:51:22 2005



From: Michael Cheatham :      mmcheath-at-mailbox.syr.edu
Date: Mon, 13 Jun 2005 07:48:59 -0400
Subject: [Microscopy] Re: lab noise control and acoustic panels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ellery,
I haven't done this with our SEM lab, but I have used sound
baffles in our Noble Gas lab. That lab has two mass specs with 7
rough pumps and 5 turbo pumps between them. Those pumps plus all of
the electronic unit cooling fans add a fair amount of noise to the
lab. I originally wanted sound baffling mounted to the walls, but
our design and construction people would not allow it. Instead they
recommended hanging 2' x 4' sound baffles from the suspended ceiling
grid. They gave me a recommended layout which I tried to follow as
best I could. The overall effect was excellent. The baffles we used
are basically a tight weave of fiberglass enclosed in a poly sealed
'bag'. No dust collects on them as happens with wall mount egg crate
material. They had two grommet wholes along one edge and they came
with standard T-bar clips to hang them. I just used zip ties to
connect the grommet to the hangers. Unfortunately, I don't know who
makes the products as our design people did all the purchasing and
had everything delivered to the lab. I hung them myself because I
don't trust our facilities people working above our instruments.

Mike


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
********************************************************************
Michael M. Cheatham
312 Heroy Geology Laboratory Phone (315)-443-1261
Syracuse University Fax (315)-443-3363
Syracuse, NY 13244-1070

email:mmcheath-at-mailbox.syr.edu
http://www.geochemistry.syr.edu

owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html
owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html
owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html
owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html
********************************************************************


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 13 09:37:14 2005



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Mon, 13 Jun 2005 09:10:33 -0500
Subject: [Microscopy] Re: M&M2005 session and noise control

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ellery and others,

I have also run into some decision making regarding acoustic panels for a
high end FESEM room. This is of major concern in a growing number of labs
installing the newer very high end TEMs and SEMs. We are going to have a
platform presentation about designing a high end facility in the Core
Facility Management session at M&M2005. The session is from 1:30 to 3:30 on
Wednesday.

I would really appreciate those who have information on acoustic noise
remedies (as well as vibration and electrical interference remedies) to
contact me. If you are going to be at M&M2005 I would invite you to
participate in this session to share this information.

Another topic that will be introduced during the session, with additional
discussion to follow the session (either at the Convention Center or at
dinner Wednesday evening) is that of LIM (large Image Management) software
and related issues (large data storage and backup, billing, authorization).

The discussion will be introduced by Avrum Goodblat and will revolve around
two major issues:

a. LIMS software
b. how to hook LIMS to the microscope. This is not always so easy to do - it
depends both on the software and the nature of the utilities provided by the
microscope manufacturer.

The Core Facility Management session, organized by the Focused Interest
Group on Facility Operation, is designed to address topics of current
interest. These threads often appear on the list serve a few months before
the actual meeting and cannot be included in the meeting program. Since the
session is geared to discussion of current topics of interest, we have a
great deal of flexibility. Please take advantage of this and plan to bring
information and participate fully in these discussions.


On 6/11/05 11:06 PM, "Ellery Frahm" {frah0010-at-tc.umn.edu} wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------------}
-
}
} Microscopy folks,
}
} Our electron microprobe laboratory has a chiller and sizable air
} conditioner, and thanks to Facilities Management at our university,
} there's little chance of getting them moved out of the lab. Since I
} cannot move the noise sources out of the lab, I've been thinking
} about installing acoustic panels to help with noise control. I've
} seen this done at a few other electron microscope labs, and the staff
} seemed happy with the results. I've been looking online at acoustic
} panels, and there are various materials, shapes, and designs out
} there. Has anyone else done this? What materials and shape did you
} use? Are you happy with the results? Any problems (shedding of
} fibers or dust, etc)? Is there anything you wish you'd done
} differently? Any suggestions or opinions are welcome.
}
} Thanks in advance,
}
} Ellery
}
} --------------------
} Ellery E. Frahm
} Research Fellow & Manager
} Electron Microprobe Laboratory
} University of Minnesota - Twin Cities
} Department of Geology & Geophysics
} Lab Website: http://probelab.geo.umn.edu/
}
}




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 13 12:15:52 2005



From: micrograph05-at-aol.com
Date: Mon, 13 Jun 2005 13:15:02 -0400
Subject: [Microscopy] LM Small World Competition - Final Call for Entries

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


www.nikonsmallworld.com

Attention Microscopists,

June 30, 2005 is the final deadline for submitting entries to the 31st
Nikon International Small World Competition. The competition, open to
all microscopists, was created in 1975 to recognize excellence in
photography through the light microscope, and to applaud the efforts of
those involved with photomicrography.

The competition is open to all types of light microscopy, and there are
no restrictions to the type of specimens or techniques used
(brightfield, darkfield, phase contrast, interference contrast,
fluorescence, confocal, deconvolution, mixed techniques, etc.).
Participants may obtain the rules, entry forms and enter their digital
images directly at http://www.nikonsmallworld.com/. For those
submitting entries in film format, the image and entry form should be
sent to: Nikon Small World, 1300 Walt Whitman Road, Melville, NY
11747-3064. The first and second of twenty prizewinners will receive a
selection of Nikon products and equipment worth $3,000 and $2,000
respectively.

Winners will be selected mid-summer. Each year, exhibits containing the
winning entries are displayed at museums and science centers throughout
North America. Many of these spectacular images have been featured on
the covers of prestigious scientific publications and journals.


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 13 13:56:22 2005



From: biology :      biology-at-ucla.edu
Date: Mon, 13 Jun 2005 11:55:37 -0700
Subject: [Microscopy] EM Position Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



http://jobs.healthcare.ucla.edu/03_header_job.html

Job Title: Histotechnologist III/EM
UCLA Title: Histotechnologist III
Job No.: H33530
Work Hours: Monday-Friday, 8:00am-5:00pm
Work Location: CHS A3-240
Minimum Salary: $20.98 / $3651
Maximum Salary: $29.37 / $5110
Layoff Referral Deadline:

Job Duties:
Provide administrative, technical, and maintenance support to the Electron
Microscopy Department. Process, embed and cut EM blocks and tissue while
maintaing excellent quality. Prepare: thin sections, staining, special
processing, preparation of knives, reviewing and scanning of grids, and
digital photography. Peforms independently a wide variety of technical and
non-technical procedures. Perform processing of primary specimens, reagent
preparation, operating, cleaning and maintaing work area and laboratory
equipment, troubleshooting specimen/test order problems, filing, answering
phones to respond to questions and participating in special
studies. Oversee staff in their assigned area to see that all procedures
meet the protocol stated. Tract laboratory supplies and places orders as
necessary. Monitoring inventories of media, supplies and reagents used in
diagnostic testing, quality control/quality assurance support, computer
oepration (report printing) and data entry and retrieval. Enter data into
the Tamtron system, maintain and print logs and labels. Perform daily
recordkeeping.


Job Qualifications:
Working knowledge of basic chemistry, biology, and anatomy with minimum of
5 years experience in Electron Microscopy. Skill in sectioning difficult
tissues. Skill in performing all ranges of testing and procedures in
Electron Microscopy. Ability to learn running variety of automated
stainers and other equipments. Skill in Tamtron system or ability to learn
very fast. Ability to maintain the work area clean and follow the safety
procedure. Ability to speak clearly and distinctly using appropriate
English vocabulary. Ability to communicate effectively with resident and
faculty in regard to the different issues. Ability to communicate with
mutual respect and consideration for diversity among individual. Ability
to work as part of team and cover the missing area voluntarily. Able to
maintain confidentiality. Willingness to participate in teleconferences
and other continuing education program. Able to modify work schedule to
meet the needs of the department. Ability to maintain the quality
control. Ability to perform any other duties or tasks as requested by the
sueprvisor.





From MicroscopyL-request-at-ns.microscopy.com Mon Jun 13 16:26:37 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 13 Jun 2005 14:25:25 -0700
Subject: [Microscopy] F30H objective aperture & low dose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Lists,
A problem has come up with using the objective aperture in conjunction
with the low dose kit. When the aperture is centered in the Exposure
state, it is off-center in the Focus state by an amount that varies
with the focus shift. The problem is the same using TEM mode or EFTEM
mode. Logically, this should be related to image shift pivot points,
but that alignment is right on, and a complete set of TEM-HM alignments
did not solve the problem. Both our local service guy and an expert
FEI guy have looked at the problem, but have not been able to solve it
(although we thought they had on a couple of occasions). Has anyone
else seen this, and, if so, what fixed it. TIA.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 13 20:11:25 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 13 Jun 2005 18:10:15 -0700
Subject: [Microscopy] Re: [3DEM] F30H objective aperture & low dose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jun 13, 2005, at 2:57 PM, Jamie Riches wrote:

} I was of the understanding that
} this problem was a fundamental one related to the image shifts and the
} objective apertures, but if you find out otherwise, could you please
} send an
} email to either of the lists?
}
Dear Lists,
According to David Mastronarde, the problem is inherent in the Tecnai,
since the obj aper is not in the back focal plane, and the alignment
procedures do not account for this.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 08:34:22 2005



From: Barbara Maloney :      maloneyb-at-fiu.edu
Date: Tue, 14 Jun 2005 09:30:36 -0400
Subject: [Microscopy] Current usage of TEM or FTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Group - what do you believe current usage for TEM or field emission
TEM is right now for biological applications?
Thanks in advance for your input
Barbara


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 09:13:37 2005



From: Sherwood, Margaret :      MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 14 Jun 2005 10:12:54 -0400
Subject: [Microscopy] Current usage of TEM or FTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Barbara,et al:

It's funny. For the first 16 years I have been at Wellman, we had two full-time
TEM people (myself included) who had non-stop projects to do. Then, about 3
years ago, (somewhat coinciding with the purchase of the confocal
microscope),TEM work dried up; literally. Now, with only myself running it, TEM
work is picking up again. I currently have 3 projects to scope. Our work is
stricly biological (I am looking at bacteria, retinal lesions and tattooed
skin). We are a large laser research center at MGH and we collaborate with a
number of the departments. (I don't just do the TEM for the group; I train
people on the confocal and am doing molecular biology as well).

We are still not up to the volume of work (TEM) that we did and probably will
never reach that level of work again, but it is nice to get back to basic
ultrastructure.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
} From: Barbara Maloney [mailto:maloneyb-at-fiu.edu]
Sent: Tuesday, June 14, 2005 9:31 AM
To: microscopy-at-msa.microscopy.com

Dear Group - what do you believe current usage for TEM or field emission
TEM is right now for biological applications?
Thanks in advance for your input
Barbara




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 11:38:13 2005



From: Pat Connelly :      psconnel-at-sas.upenn.edu
Date: Tue, 14 Jun 2005 12:17:00 -0400
Subject: [Microscopy] Re: Current usage of TEM or FTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Dear Group - what do you believe current usage for TEM or field emission TEM
} is right now for biological applications?
} Thanks in advance for your input
} Barbara {maloneyb-at-fiu.edu}
==========================
Barbara,
I am currently the only TEM Technologist in my biology department and
since my PI is retiring in September it will also be the end of my
employment here. Over the past several years we have had some
collaborators who could not get research quality TEM done in their
own departments or institutions. They realized that biochemistry,
confocal microscopy, etc. showed that the experiments were working
but could not give the ultrastructural confirmation that was
ultimately needed for understanding and hence to get the publication
of their data accepted. More than once I have heard the words, "That
is exactly what I thought was happening but I could not prove it."

A grant request has been made for a new TEM for our department, the
present scope being 39 years old. Ten investigators are making plans
to use the scope (hoping that the grant is funded) so it seems that
there is still a need for TEM [and I might add that I am attempting
to make known to them that it may be wise to have an experienced
technologist around!].

For these reasons, I state that TEM has not been abandoned only "put
on a back burner" while other techniques have caught the glamor of
the day. As more investigators see the importance of confirming
their results on the ultrastructural level or re-discover the sense
of wonder at seeing what has not been observed or reported
previously, TEM will regain its place in biological applications.

Just my thoughts and feelings,
Pat Connelly psconnel-at-sas.upenn.edu
Dept. of Biology
Univ. of Pennsylvania
Philadelphia, PA






From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 12:36:54 2005



From: :      nyilmaz-at-mersin.edu.tr
Date: Tue, 14 Jun 2005 20:36:09 +0300
Subject: [Microscopy] staphylococcus suspension protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everybody...

I'm searching a protocol processing staphylococcus suspension for TEM.

Thanks in advance...

Dr. Nejat Yilmaz







From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 13:20:43 2005



From: Leona Cohen-Gould :      lcgould-at-med.cornell.edu
Date: Tue, 14 Jun 2005 14:19:55 -0400
Subject: [Microscopy] Re:mouse embryo SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,
I was just asked about how to process mousse embryos (embryonic days
9.5, 10.5 and 11.5) for SEM specifically to look at the limb buds.
I"ve done TEM of limb buds, but not SEM. Would anyone out there care
to suggest a protocol (if it differs substantially from "standard"
fixation-dehydration and CPD)?
Thanks,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 14:25:21 2005



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Tue, 14 Jun 2005 15:24:35 -0400
Subject: [Microscopy] Re: Re:mouse embryo SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Lee,
When we processed mouse embryos for SEM ( in '92) we had excellent results
using "standard" fixation, dehydration and CPD.
Frank

At 02:19 PM 6/14/2005, Leona Cohen-Gould wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 14:55:19 2005



From: Rhonda Stroud :      stroud-at-nrl.navy.mil
Date: Tue, 14 Jun 2005 15:54:26 -0400
Subject: [Microscopy] TEM/FIB postdoc position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



The Materials Science Division at the Naval Research Laboratory in
Washington, DC, seeks postdoctoral fellow candidates to carry out TEM and
FIB-based investigations of nanostructured materials. Potential projects
include analysis of natural and synthetic nanoparticles, e.g., 1 to 10 nm
particles condensed in the upper atmosphere and/or laboratory grown by
reverse micelle synthesis; FIB-based micromanipulation and analysis of
cosmic dust samples (STARDUST Mission and dust from stars pre-dating the
Sun), and in situ STM-TEM studies of nanowires. NRL has extensive
facilities for microscopy studies, including newly installed JEOL 2200FS
and FEI Nova 600 DB-FIB instruments. Experience in HAADF imaging, EDS and
EELS spectrum imaging and/or FIB operation are desired. A Ph.D. in
physics, chemistry, materials science, or geology is required. Qualified
candidates will be expected to submit a proposal for the August 1, NRC
fellowship deadline, but start dates prior to the October completion of the
review process are possible. US citizens or permanent residents only. For
more information, contact Rhonda Stroud (stroud-at-nrl.navy.mil).




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 15:33:00 2005



From: David Elliott :      elliott-at-arizona.edu
Date: Tue, 14 Jun 2005 13:32:12 -0700
Subject: [Microscopy] Re: Re:mouse embryo SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

http://www3.interscience.wiley.com/cgi-bin/abstract/102524606/ABSTRACT
Has the SEM embedding procedures

Grose R, Harris BS, Cooper L, Topilko P, Martin P. 2002. Immediate
early genes krox-24 and krox-20 are rapidly up-regulated after
wounding in the embryonic and adult mouse. Dev Dyn 223: 371-378. Links
Has the exact fix

David



On Jun 14, 2005, at 11:19 AM, Leona Cohen-Gould wrote:

}
}
} ----------------------------------------------------------------------
} --------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/
} MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ---------
}
} Hi All,
} I was just asked about how to process mousse embryos (embryonic
} days 9.5, 10.5 and 11.5) for SEM specifically to look at the limb
} buds.
} I"ve done TEM of limb buds, but not SEM. Would anyone out there
} care to suggest a protocol (if it differs substantially from
} "standard" fixation-dehydration and CPD)?
} Thanks,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy & Histology Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
}



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 15:35:55 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 14 Jun 2005 13:34:46 -0700
Subject: [Microscopy] Re: Current usage of TEM or FTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jun 14, 2005, at 6:30 AM, Barbara Maloney wrote:

} what do you believe current usage for TEM or field emission TEM is
} right now for biological applications?
} Thanks in advance for your input

Dear Barbara,
All structural biology benefits from TEM (although not all uses it).
We are doing both tomography, for high-resolution 3-D imaging of
biological specimens, and single-particle analysis, for
very-high-resolution determination of the structures of protein
complexes. Almost all our work in on frozen-hydrated unstained
specimens, which is as close to the native state as can presently be
achieved. So the bottom line is that (FE)TEM is the best technique for
determining the high-resolution structure of (nearly) native biological
specimens.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 14 15:39:49 2005



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Tue, 14 Jun 2005 21:35:41 +0100
Subject: [Microscopy] Re: lab noise control and acoustic panels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Curtains? Works well in many cases and a lot cheaper than specialist
panels. If they don't work, you won't have lost much; if they do,
you'll probably have saved quite a bit.
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 15 11:39:16 2005



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Wed, 15 Jun 2005 13:13:57 -0400
Subject: [Microscopy] LM fish eggs processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everybody,
I have a user who wants to process fish eggs (salmonids) for LM.
He embedded eggs in paraffin but infiltration was insufficient and
samples could not be cut at all. Is there a better protocol to handling
these kind of samples? We tried Spurr with dehydration time
extended to 1h in each concentration of alcohol and resin infiltration
overnight. Still did not work. The interior was not properly infiltrated
and sample was shrunk.
Thanks
Dorota


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 15 15:07:20 2005



From: Larry Stoter :      larry-at-cymru.freewire.co.uk
Date: Wed, 15 Jun 2005 20:48:37 +0100
Subject: [Microscopy] Re: Re: lab noise control and acoustic panels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} On 6/14/05 3:35 PM, "Larry Stoter" {larry-at-cymru.freewire.co.uk} wrote:
}
} }
} ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ------------------------------------------------------------------------------}
} -
} }
} } }
} -----------------------------------------------------------------------------} }
} -
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } } -----------------------------------------------------------------------------
} } } --
} } }
} } } Microscopy folks,
} } }
} } } Our electron microprobe laboratory has a chiller and sizable air
} } } conditioner, and thanks to Facilities Management at our university,
} } } there's little chance of getting them moved out of the lab. Since I
} } } cannot move the noise sources out of the lab, I've been thinking
} } } about installing acoustic panels to help with noise control. I've
} } } seen this done at a few other electron microscope labs, and the
} } } staff seemed happy with the results. I've been looking online at
} } } acoustic panels, and there are various materials, shapes, and
} } } designs out there. Has anyone else done this? What materials and
} } } shape did you use? Are you happy with the results? Any problems
} } } (shedding of fibers or dust, etc)? Is there anything you wish you'd
} } } done differently? Any suggestions or opinions are welcome.
} } }
} } } Thanks in advance,
} } }
} } } Ellery
} } }
} } } --------------------
} } } Ellery E. Frahm
} } } Research Fellow & Manager
} } } Electron Microprobe Laboratory
} } } University of Minnesota - Twin Cities
} } } Department of Geology & Geophysics
} } } Lab Website: http://probelab.geo.umn.edu/
} }
} } Curtains? Works well in many cases and a lot cheaper than specialist
} } panels. If they don't work, you won't have lost much; if they do,
} } you'll probably have saved quite a bit.

} Larry,
} Can you provide any specifics about curtains such as what to look for,
} possible sources, etc.
}
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}

Nothing special - in the JEOL UK Applications Lab we just went to the
local department store and ordered up some curtains of the correct
size and hung them against the wall, ceiling to floor. Went for a
fairly heavy, non-synthetic material, as we thought synthetics might
cause problems with static electricity. Even to my ears, the room
acoustics were very noticeably deadened. They weren't exactly cheap,
since we needed quite a lot and it was quite a heavy material. On the
other hand, I'd bet it cost less than specialist acoustic tiles.

--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 15 20:08:38 2005



From: dgarrett-at-unt.edu (by way of MicroscopyListserver)
Date: Wed, 15 Jun 2005 20:07:54 -0500
Subject: [Microscopy] viaWWW: SEM general JEOL 5800 CMD list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dgarrett-at-unt.edu) from http://www.microscopy.com/MLFormMail.html on Wednesday, June 15, 2005 at 10:06:28
---------------------------------------------------------------------------

Email: dgarrett-at-unt.edu
Name: Da vid Garrett

Organization: University of North Texas

Title-Subject: [Microscopy] [Filtered] SEM general JEOL 5800 CMD list

Question: Can anyone supply me with a list of the features that are controled with the CMD key. To change the load current press CMD type bias use focus and mag to change. Thats the only one I know.
Thanks, David

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 16 07:41:24 2005



From: chenkaic-at-msu.edu (by way of MicroscopyListserver)
Date: Thu, 16 Jun 2005 07:40:41 -0500
Subject: [Microscopy] viaWWW: EPON and Prion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (chenkaic-at-msu.edu) from http://www.microscopy.com/MLFormMail.html on Wednesday, June 15, 2005 at 23:27:20
---------------------------------------------------------------------------

Email: chenkaic-at-msu.edu
Name: Kai-Chun

Organization: MSU

Title-Subject: [Microscopy] [Filtered] EPON and Prion

Question: Dear all,

My advisor and I want to visulize prion protein with EM.
We want to use negative stainning.
However, it is somewhat difficult because of the safety level of EM lab, so we are thinking about how to deactivate prion.
I heard that EPON may deactivate prion, but I can not find the references to support that.
I wonder if anyone has this kind of experiences to handle prion protein.
Do you think EPON can deactivate prion protein?

Thank you very much!

Kai-Chun



---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 16 09:22:06 2005



From: paul r hazelton :      paul_hazelton-at-umanitoba.ca
Date: Thu, 16 Jun 2005 09:21:21 -0500
Subject: [Microscopy] Re: prions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

help!!!

i seem to have lost a very good response to the recent posting asking
about prion infectivity - raised by ralph common. someone on the list
responded with information from previous work of theirs, where they had
checked to determine retention of catalytic infectivity of prions after
osmication.

i seem to not have that particular response saved. could any one on the
list who has the note, or the original respondant, please send me
another copy - it was excellent, probably the best of the whole group of
responses. i wish to have it in my notes for future reference.

thanks

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 16 09:27:58 2005



From: Aleksandr Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Thu, 16 Jun 2005 15:27:40 +0100
Subject: [Microscopy] Re: viaWWW: EPON and Prion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Kai-Chun,

} Question: Dear all,
}
} My advisor and I want to visulize prion protein with EM.
} We want to use negative stainning.
} However, it is somewhat difficult because of the safety level of EM lab, so we are thinking about how to deactivate prion.
} I heard that EPON may deactivate prion, but I can not find the references to support that.
} I wonder if anyone has this kind of experiences to handle prion protein.
} Do you think EPON can deactivate prion protein?
}
}
IMHO, the main danger when you work with prion protein infected tissue
is the possible inhalation or ingestion of the contaminated material.
Therefore, if you will cover the grid with some strong film then it will
in theory protect the environment from the prion exposure. However, all
the films have the tendency to break at some point that makes their
protection questionable. The idea of covering the grid with Epon may
work because Epon is quite resistant material. However in this case the
resolution can be decreased because of the thickness of epoxy film
cover. The best solution to see negatively stained prion protein on the
grid would be dedicated microscope. It is not cheap solution but it is safe.
If you will embedd your protein in epon and then make sections then
probably this protein would be inactive, but I do not have any
references that prove it.
You may want to contact Dr. Holger Wille from S.Prusiner lab (UCSF) who
has vast experience with prion proteins in EM.

Sincerely,

--
Aleksandr Mironov
Experimental Officer
G450A, Stopford Building
EM Unit, Faculty of Life Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-7395
Fax. +44-(0)161-275-5171
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
MSN: amironov-at-hotmail.co.uk
Web: http://www.empgu.man.ac.uk




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 16 09:37:01 2005



From: Karl Hagglund :      hagglundk1-at-nku.edu
Date: Thu, 16 Jun 2005 10:36:19 -0400
Subject: [Microscopy] Need JEE 4B coater parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've brought an old high vacuum coater back to life, and it is working
well for carbon coating, but I am missing the parts to do metal coatings
and set up an aperture cleaning boat.

The unit is a Jeol JEE 4B, manufactured around 1972. What I am looking
for is not all that different from ring stand parts, and I wonder
whether anyone out there has modified ring stand parts to use with a
high vacuum coater. It seems that all I would need is a couple of
insulators and a tap and die set to attach a piece of wire or moly boat
and get going.

Otherwise, is there a third party company that makes parts that would
fit on one of these?

Thanks for your help.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 16 10:29:30 2005



From: Barbieri Thomas-r53545 :      Thomas.Barbieri-at-freescale.com
Date: Thu, 16 Jun 2005 08:28:45 -0700
Subject: [Microscopy] home-built voltage contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

We'd like to scratch-build an apparatus that allows us to perform voltage contrast testing in one of our SEMs. We currently have a Hitachi S-4500, a JEOL 6301, and a JEOL 6340. Does anyone on here have experience using or building such a system? If you do, could you provide a description of the components and provide hints and tips on assembling one?

Regards,
Tom

Thomas Barbieri
Arizona Product Development and Analysis Laboratory
Freescale Semiconductor
Tempe, AZ
480-413-4007

thomas.barbieri-at-freescale.com

The information contained in this email and any attachment is considered:
[X] General Business Information
[ ] Freescale Internal Use Only
[ ] Freescale Confidential Proprietary


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 16 10:32:53 2005



From: Bill Powell :      whpowell-at-sbcglobal.net
Date: Thu, 16 Jun 2005 12:24:57 -0400
Subject: [Microscopy] Need JEE 4B coater parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Paul & Listers:

It was me...here is the text from that thread...


Hi All:

Having started my professional career managing a EM lab working on CJD I
have actual experience working with this fun stuff. We found that PF &
Glut perfusions did not inactivate the mutant protein. Once the tissue was
post-fixed in OsO4 we could not serial pass the disease again. Once in
plastic it's for all intents and purposes inert. Waste materials were
autoclaved for a longer length of time than normal and discarded with
"normal" medical waste. In the early 80's when I was working with CJD it
was a BSL2 for the most part, now all procedures should be done under BSL3.
The Neuropathologist used to work with it under BSL1, removing the CJD
infected brains from the cadavers. They would always be violating
containment protocols in some was shape or form. They are still alive,
except one (CVA). The point I'm trying to make is don't over react, take
all of the prudent precautions of BSL3 you will be well protected.


Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com

----- Original Message -----
} From: "paul r hazelton" {paul_hazelton-at-umanitoba.ca}
To: "Ralph Common" {Ralph.Common-at-hc.msu.edu}
Cc: {Microscopy-at-microscopy.com}
Sent: Thursday, June 16, 2005 10:21 AM

Karl,

Thank you for the conversation this afternoon.

Items for the JEE-4B may be available from our Parts Department (Nancy
Green). Instruction manuals (Ion Gauge) may be a bit more difficult however
we may be able to provide copies.

The main number to JEOL USA Inc in Peabody Massachusetts is 978-535-5900,
please ask for Nancy Green.

I will let you know when we have fixed the schedule for UC on the JEM-1230
demonstration.

Nice to have spoken with you.

Regards,

Bill Powell
JEOL USA Inc
Phone: 248-366-8351
Ion Beam Cross Section Polisher:
http://www.jeol.com/spe/speprods/cross_section.html


-----Original Message-----
} From: Karl Hagglund [mailto:hagglundk1-at-nku.edu]
Sent: Thursday, June 16, 2005 10:36 AM
To: microscopy-at-microscopy.com

I've brought an old high vacuum coater back to life, and it is working
well for carbon coating, but I am missing the parts to do metal coatings
and set up an aperture cleaning boat.

The unit is a Jeol JEE 4B, manufactured around 1972. What I am looking
for is not all that different from ring stand parts, and I wonder
whether anyone out there has modified ring stand parts to use with a
high vacuum coater. It seems that all I would need is a couple of
insulators and a tap and die set to attach a piece of wire or moly boat
and get going.

Otherwise, is there a third party company that makes parts that would
fit on one of these?

Thanks for your help.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu






From MicroscopyL-request-at-ns.microscopy.com Thu Jun 16 12:28:25 2005



From: Evelyn York :      eyork-at-ucsd.edu
Date: Thu, 16 Jun 2005 10:27:43 -0700
Subject: [Microscopy] Re: home-built voltage contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tom,

There are more then a few variables to consider when you decide to utilize
voltage contrast. For example, in addition to the tooling and fixtures and
SEM, the device type and fabrication process play a huge role in how
successful you can be at capturing the correct electron signals. Please
contact me off line and I'll share whatever experience and knowledge I have.

Regards,
Evelyn


At 08:28 AM 6/16/2005, Barbieri Thomas-r53545 wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Evelyn York

Analytical Facility
Scripps Institution of Oceanography
University of California, San Diego
9500 Gilman Drive M/S 208
La Jolla, CA 92093-0208

(858) 534-2438



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 16 21:03:37 2005



From: emlab-at-vet.ksu.edu (by way of MicroscopyListserver)
Date: Thu, 16 Jun 2005 21:02:54 -0500
Subject: [Microscopy] viaWWW: transferrine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (emlab-at-vet.ksu.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, June 16, 2005 at 08:51:27
---------------------------------------------------------------------------

Email: emlab-at-vet.ksu.edu
Name: Lloyd Willard, EM Lab

Organization: Kansas State University

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear All,

Could anyone give me the name of a company that sells/distributes cold labeled transferrine (10nm preferably).

Thank you very much!

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 17 01:02:38 2005



From: :      Colin.Veitch-at-csiro.au
Date: Fri, 17 Jun 2005 16:01:54 +1000
Subject: [Microscopy] Film thickness measurements with EDXS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

A colleague has some thin metal films (5-50nm) on Si wafers and we need
to know the relative film thicknesses. We don't need to know the
absolute thickness. Is there any way to use the EDXS signal to get
relative thickness? I.e. use the suppression of the Si signal? Or will
the results be too unreliable? We have no other way here to do the job.

Any suggestions (including don't bother trying it!!!) will be welcome.

Cheers.

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.




From MicroscopyL-request-at-ns.microscopy.com Fri Jun 17 07:55:19 2005



From: Kestutis Smalinskas :      smalinskas-at-yahoo.com
Date: Fri, 17 Jun 2005 05:54:37 -0700 (PDT)
Subject: [Microscopy] Re: Film thickness measurements with EDXS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colin,

This exercise should be rather straightforward. I've
done it a number of times. If the film is thin
enough, you'll pick up the Si under the metal film.
The thinner the film, the stronger the Si peak. This
will vary with accelerating voltage. Find a voltage
that picks up both the metal film and Si with
reasonably large peaks. To compare the two samples
you'll need to collect a spectrum from each sample
using the same accelerating voltage constant. Then
compare the two spectra looking at the ratio of
metal/Si peak heights within each spectrum.

To take it a step futher, you could get absolute
values if you had control samples with known metal
thickness for comparison.

Stu Smalinskas, P.E.
SKF North American Technical Center
Plymouth, MI
(734) 414-6862
stu.smalinskas-at-skf.com

--- Colin.Veitch-at-csiro.au wrote:

}
}
}
------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
-------------------------------------------------------------------------------
}
} Hi,
}
} A colleague has some thin metal films (5-50nm) on Si
} wafers and we need
} to know the relative film thicknesses. We don't
} need to know the
} absolute thickness. Is there any way to use the
} EDXS signal to get
} relative thickness? I.e. use the suppression of the
} Si signal? Or will
} the results be too unreliable? We have no other way
} here to do the job.
}
} Any suggestions (including don't bother trying
} it!!!) will be welcome.
}
} Cheers.
}
} Colin Veitch
}
} Electron Microscopist
}
} CSIRO Textile and Fibre Technology
}
} PO Box 21, BELMONT, Vic. 3216. Australia.
}
} E-mail: colin.veitch-at-csiro.au
}
} Web: http://www.tft.csiro.au
}
} Tel: +61 (0) 3 5246 4000
} Mob: 0438 538 475
} Fax: +61 (0) 3 5246 4811
}
}
}
} The information contained in this e-mail message may
} be privileged or
} confidential information. If you are not an intended
} recipient, you may
} not copy, distribute or take any action in reliance
} on it. If you have
} received this message in error, please telephone
} CSIRO Textile and Fibre
} Technology on +61 3 5246 4000.
}
}
}
}




__________________________________
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From MicroscopyL-request-at-ns.microscopy.com Fri Jun 17 08:08:03 2005



From: Chaoying Ni :      cni-at-UDel.Edu
Date: Fri, 17 Jun 2005 09:07:15 -0400 (EDT)
Subject: [Microscopy] Re: Film thickness measurements with EDXS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There are plenty of ways to do this. Immediately coming up in my mind is
the 4-point resistivity measurement with a routine device in any chip
manufacturing shop. x-section SEM should you have a hi-res SEM and be
able to afford for the destruction of the sample or x-section TEM is the
other

****************************************
Chaoying Ni, PhD
The W.M. Keck Electron Microscope Facility
Facility location: 022 Spencer Laboratory
Mailing address:
201 duPont Hall
Dept of Matls Sci & Eng
University of Delaware
Newark, DE 19716
(302) 831-2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************

On Fri, 17 Jun 2005 Colin.Veitch-at-csiro.au wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Hi,
}
} A colleague has some thin metal films (5-50nm) on Si wafers and we need
} to know the relative film thicknesses. We don't need to know the
} absolute thickness. Is there any way to use the EDXS signal to get
} relative thickness? I.e. use the suppression of the Si signal? Or will
} the results be too unreliable? We have no other way here to do the job.
}
} Any suggestions (including don't bother trying it!!!) will be welcome.
}
} Cheers.
}
} Colin Veitch
}
} Electron Microscopist
}
} CSIRO Textile and Fibre Technology
}
} PO Box 21, BELMONT, Vic. 3216. Australia.
}
} E-mail: colin.veitch-at-csiro.au
}
} Web: http://www.tft.csiro.au
}
} Tel: +61 (0) 3 5246 4000
} Mob: 0438 538 475
} Fax: +61 (0) 3 5246 4811
}
}
}
} The information contained in this e-mail message may be privileged or
} confidential information. If you are not an intended recipient, you may
} not copy, distribute or take any action in reliance on it. If you have
} received this message in error, please telephone CSIRO Textile and Fibre
} Technology on +61 3 5246 4000.
}
}
}
}


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 17 10:19:21 2005



From: Jeff Stewart :      jeff-at-metallography.com
Date: Fri, 17 Jun 2005 11:12:59 -0400
Subject: [Microscopy] International Metallographic Contest

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's not too late to enter the International Metallographic Contest and
Exhibit co-sponsored since 1972 by the International Metallographic Society
and ASM International. The contest will be held in conjunction with the 38th
annual technical meeting of the IMS and the M&M 2005 meeting this August in
Honolulu. Twelve categories of competition. Best in show receives $3000 and
the prestigious Jaquet-Lucas Award. Cash awards for first, second, and third
place winners in eleven of the categories. Entries are prominently displayed
during the M&M 2005 meeting and again in the fall during the ASM Annual
Event. Deadline for entries is July 18. For additional information including
rules, tips for creating a winning entry, judging guidelines, and examples
of winning entries contact me or visit
http://www.internationalmetallographicsociety.org/contest.html.

Jeff Stewart
International Metallographic Contest Chair
Metallographic Laboratory Manager
Stern-Leach Co.
49 Pearl Street
Attleboro, MA 02703 USA
Phone: 508-222-7400 extension 1329
FAX: 508-699-4030




From MicroscopyL-request-at-ns.microscopy.com Fri Jun 17 13:00:34 2005



From: diller-at-stefan-diller.com (by way of MicroscopyListserver)
Date: Fri, 17 Jun 2005 12:59:53 -0500
Subject: [Microscopy] viaWWW: image database of herbs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-stefan-diller.com) from http://microscopy.com/MLFormMail.html on Thursday, June 16, 2005 at 08:35:16
---------------------------------------------------------------------------

Email: diller-at-stefan-diller.com
Name: Stefan Diller

Organization: Photography

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Dear All,
does anybody know of an image database in the net, preferably freely accessible, which deals with herbs and especially with the microstructure of herbs using SEM and TEM?

Best regards,
Stefan


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 17 13:02:21 2005



From: Lou Ross :      RossLM-at-missouri.edu
Date: Fri, 17 Jun 2005 13:01:36 -0500
Subject: [Microscopy] Re: Film thickness measurements with EDXS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Colin,

For years I have used a free shareware program called GMRfilm to
measure submicrometer film thicknesses on substrates. It is an old
DOS based program (I think there might be newer programs out now) and
can be found on the MSA website through the reference and educational
link or at http://www.amc.anl.gov. From the parent directory, go to
02-MMSLib/ XEDS/ GMRFILM/.

I routinely use it to calibrate coating thicknesses for our sputter
coater and vacuum evaporator. As Stu mentioned, you need to choose
the appropriate keV to excite both the x-ray signal from the film and
the substrate. Then under identical conditions measure collect
spectra from pure standards. Extract the net peak intensities and
calculate the K-ratios. Once you have the K-ratios and other
operating and specimen parameters are entered into GMRfilm to
calculate the film thickness.

If you want, feel free to contact me off line for more information.

Lou Ross




}
} Hi,
}
} A colleague has some thin metal films (5-50nm) on Si wafers and we need
} to know the relative film thicknesses. We don't need to know the
} absolute thickness. Is there any way to use the EDXS signal to get
} relative thickness? I.e. use the suppression of the Si signal? Or will
} the results be too unreliable? We have no other way here to do the job.
}
} Any suggestions (including don't bother trying it!!!) will be welcome.
}
} Cheers.
}
} Colin Veitch
}
} Electron Microscopist
}
} CSIRO Textile and Fibre Technology
}
} PO Box 21, BELMONT, Vic. 3216. Australia.
}
} E-mail: colin.veitch-at-csiro.au
}
} Web: http://www.tft.csiro.au
}
} Tel: +61 (0) 3 5246 4000
} Mob: 0438 538 475
} Fax: +61 (0) 3 5246 4811
}
}
}
} The information contained in this e-mail message may be privileged or
} confidential information. If you are not an intended recipient, you may
} not copy, distribute or take any action in reliance on it. If you have
} received this message in error, please telephone CSIRO Textile and Fibre
} Technology on +61 3 5246 4000.


--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=2227
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 17 15:33:55 2005



From: sschmech-at-u.washington.edu (by way of MicroscopyListserver)
Date: Fri, 17 Jun 2005 15:33:12 -0500
Subject: [Microscopy] viaWWW: strange cytoplasmic changes : reovirus effects on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sschmech-at-u.washington.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, June 17, 2005 at 15:22:09
---------------------------------------------------------------------------

Email: sschmech-at-u.washington.edu
Name: Steve Schmechel

Organization: University of Washington

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: In a study of reovirus effects on infected cells in culture, we are finding strange cytoplasmic changes. Cells infected with some strains (and not others) have strange cytoplasmic structures. These are non-membrane bound clear spaces that appear as wide splits in the cytoplasm. They are large, approximatly the length of the nucleus with a width approximately one fifth of length. They "smile" at the middle and are curved. Although none are directly next to the nucleus, they take a curved form that generally follows a similar curve as the nuclear membrane. In most cells there are many of these. The result is that the cells have a zebra stripe appearance due to these multiple splits in the cytoplasm.

Has anyone ever seen something like this. I'm happy to email you a sample if you like.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 17 16:33:23 2005



From: Edward Calomeni :      calomeni-1-at-medctr.osu.edu
Date: Fri, 17 Jun 2005 17:32:13 -0400
Subject: [Microscopy] Re:reovirus effectson infected cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Steve,

Without an image to study, my first guess is that the 'inclusions' are
cholesterol clefts.
Frank Ramig, at Baylor College of Medicine, Division of Biochemical
Virology has many, many years under his belt in working with reoviruses.
He might be a good person to ask about these things.

Best of luck,

Ed

Edward Calomeni
Director EM Lab
Ohio State University - Pathology
M018 Starling Loving Hall
320 W. 10th Ave.
Columbus, OH 43210-1240
614-293-5580 (office)
614-293-8806 (lab)
calomeni-1-at-medctr.osu.edu

} } } by way of MicroscopyListserver {sschmech-at-u.washington.edu}
6/17/2005 4:33 PM } } }


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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sschmech-at-u.washington.edu) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
June 17, 2005 at 15:22:09
---------------------------------------------------------------------------

Email: sschmech-at-u.washington.edu
Name: Steve Schmechel

Organization: University of Washington

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: In a study of reovirus effects on infected cells in culture,
we are finding strange cytoplasmic changes. Cells infected with some
strains (and not others) have strange cytoplasmic structures. These are
non-membrane bound clear spaces that appear as wide splits in the
cytoplasm. They are large, approximatly the length of the nucleus with
a width approximately one fifth of length. They "smile" at the middle
and are curved. Although none are directly next to the nucleus, they
take a curved form that generally follows a similar curve as the nuclear
membrane. In most cells there are many of these. The result is that
the cells have a zebra stripe appearance due to these multiple splits in
the cytoplasm.

Has anyone ever seen something like this. I'm happy to email you a
sample if you like.

---------------------------------------------------------------------------





From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 08:17:28 2005



From: Gervais.Sawyer-at-bcuc.ac.uk (by way of Ask-A-Microscopist)
Date: Mon, 20 Jun 2005 08:16:47 -0500
Subject: [Microscopy] AskAMicroscopist: SEM image OF ash structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Gervais.Sawyer-at-bcuc.ac.uk) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, June 20, 2005 at 04:56:39
---------------------------------------------------------------------------

Email: Gervais.Sawyer-at-bcuc.ac.uk
Name: Gervais Sawyer

Organization: BCUC, UK

Education: Graduate College

Location: High Wycombe, Buckinghamshire, UK

Question: I am trying to SEM image an ash structure after burning pieces of wood. These are extremely beautiful and could reveal some microstructure. However, the ash is very delicate and disintegrates when I go to vacuum. The same thing happens when I try to sputter coat. Any ideas please?

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 08:53:09 2005



From: Kerrie Venner :      k.venner-at-ion.ucl.ac.uk
Date: Mon, 20 Jun 2005 14:52:19 +0100
Subject: [Microscopy] TEM digitisation: digital plates vs digital camera systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been given the unenviable task of having to decide between a digital
camera system and a digital imaging plate system (the Ditabis system) to
enable our older TEMs to become fully digital imaging systems. We have Jeol
1200EX and 100CX instruments and a Phillips CM10. Has anyone had experience
of the Ditabis system? In the UK there is only one currently in use, and I
have contact with that person. Our samples are biological samples, some
routine clinical specimens, but also research samples and cell culture
samples. We currently use Kodak em film 4489 and require the replacement
system to have comparable resolution.
Any comments would be gratefully received.






From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 11:40:01 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 20 Jun 2005 09:38:27 -0700
Subject: [Microscopy] Re: TEM digitisation: digital plates vs digital camera systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jun 20, 2005, at 6:52 AM, Kerrie Venner wrote:

} We currently use Kodak em film 4489 and require the replacement
} system to have comparable resolution.
} Any comments would be gratefully received.

Dear Kerrie,
The grain size on 4489 is extremely small (~1 um), so the resolution
is much better than can be achieved with any image plate system. Image
plates have many advantages over film, such as linearity and ease of
digitization, so they are better than film for quantitation, but for
enlargement by many times or other processes that require high
resolution, film is still superior. I do not know any specifics about
the Ditabis system.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 11:58:31 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Mon, 20 Jun 2005 09:57:35 -0700
Subject: [Microscopy] TEM digitisation: digital plates vs digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kerrie;
There is an operational difficulty with imaging plate systems in
that magnification and serial number are not recorded on the plate. In
fact, nothing is recorded on the plate except the electron image. Think
about getting one plate out of order, and how that might foul up your
operations.

John Mardinly
Intel

This opinion is that of the author and does not represent the opinion of
Intel corporation.

-----Original Message-----
} From: Kerrie Venner [mailto:k.venner-at-ion.ucl.ac.uk]
Sent: Monday, June 20, 2005 6:52 AM
To: Microscopy-at-microscopy.com

I have been given the unenviable task of having to decide between a
digital
camera system and a digital imaging plate system (the Ditabis system) to
enable our older TEMs to become fully digital imaging systems. We have
Jeol
1200EX and 100CX instruments and a Phillips CM10. Has anyone had
experience
of the Ditabis system? In the UK there is only one currently in use,
and I
have contact with that person. Our samples are biological samples, some
routine clinical specimens, but also research samples and cell culture
samples. We currently use Kodak em film 4489 and require the
replacement
system to have comparable resolution.
Any comments would be gratefully received.








From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 13:05:13 2005



From: Hong Yi :      hyi-at-emory.edu
Date: Mon, 20 Jun 2005 14:04:31 -0400
Subject: [Microscopy] JB-4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Can anyone tell me if JB-4 embedded sample can be sectioned on an
ultramicrotome (Leica Ultracut S), and what the appropriate thickness
should it be cut at? Thank you in advance.

HONg
Emory



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 13:24:48 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Mon, 20 Jun 2005 14:24:03 -0400
Subject: [Microscopy] Re: JB-4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hong,
I think JB-4 is a species of methacrylate resin so sure you
can cut it on an Ultra. I'd guess anywhere from 2 um semis right down
to 60nm ultras (though it may be difficult to get all the way that
thin) depending on what you want to do.

Happy slicing,
Tobias

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 13:40:10 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Mon, 20 Jun 2005 13:39:23 -0500
Subject: [Microscopy] Re: JB-4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have cut 0.5 to 2 um thick JB-4 sections using a dry glass knife without
a boat on many occasions. As the section starts to come off on the knife
face, help it along with one of the tines of a fine forceps so that the
section doesn't crumble up. Gently lift the cut section and drop in one
motion on a drop of water. be sure to let go of the section before it
touches the water or it will shrivel up against the forceps. Heat the
section down on a hot plate as one would do for an Epon section. It works
but is tedious. Furthermore, I would say the frustration is unnecessary
since BMMA (butyl-methyl methacrylate/methyl methacrylate mixtures) are
less expensive (generic ingredients), easier to cut (cuts on water filled
boats), and can be deplasticized using acetone. We love BMMA and haven't
used JB-4 ever since the esteemed Dr. Tobias Baskin told us about it. I
don't have the reference to his paper but he monitors this listserver and
perhaps will post it.



At 01:04 PM 06/20/05, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 13:45:12 2005



From: Brendan J Griffin :      bjg-at-cmm.uwa.edu.au
Date: Mon, 20 Jun 2005 13:44:28 -0500
Subject: [Microscopy] Positon Open : nanoSIMS 50 ion probe position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear all

Please draw the following position description to the attention of
any potentially interested people. We can be flexible for the right
person, within usual constraints. We have just won a new WA State
Government Centre of Excellence grant that will provide an additional
position into the nanoSIMS laboratory later in the year or early next
year.

Advertised in: The West Australian (Prof Appts) Saturday 11 June 2005
The Australian (HES) Wednesday 15 June 2005


RESEARCH OFFICER/SENIOR RESEARCH OFFICER (REF: 885)
CENTRE FOR MICROSCOPY AND MICROANALYSIS

o Ongoing appointment
o Salary range: HEE Level 6 $50,795 - $53,762 p.a.
o Salary range: HEE Level 7 $55,739 - $62,000 p.a.
o Closing date: Tuesday, 28 June 2005

A CAMECA nanoSIMS 50 high-resolution ion microprobe was installed in
the Centre for Microscopy and Microanalysis (CMM) at The University
of Western Australia, as part of the NANO-MNRF, in June, 2003. The
NANO-MNRF (www.nano.org.au) is a Major National Research Facility
that links advanced nano-scale characterisation equipment at the
Universities of Sydney, New South Wales, Queensland, Western
Australia (UWA) and Melbourne. The range of CMM microscopy
instrumentation and expertise is extensive (see
http://cmm.uwa.edu.au) such that the centre and MNRF represent a
premium environment for research services, research training and
research programs. UWA is also a major partner in local research
consortia in isotope science with a range of stable and radiogenic
isotope facilities, including two SHRIMP-II.

We are seeking a highly motivated, self-guided scientist who has the
ability to work with other staff within the Centre and its users. The
prime responsibility will be to manage a CAMECA nanoSIMS 50 ion
microprobe as a world-class National Facility. The position will have
the core role in a strong team led locally by Associate Professor
Brendan Griffin and nationally by Professor Simon Ringer (NANO MNRF
Executive Director). This position is advertised as a Level 6 or 7
depending on qualifications and experience. The minimum qualification
is a relevant degree or equivalent experience.

Application Details: Interested applicants must obtain the
application package and address the prerequisites and selection
criteria. These essential details can be accessed from the vacancy
page on http://jobs.uwa.edu.au/ or the 24 hour "hotline" on 6488
3733. To discuss or clarify any aspects of the position please
contact the director of the CMM, Associate Professor Brendan Griffin
on (08) 6488 2770 or email bjg-at-cmm.uwa.edu.au.

Committed to recruiting, developing and retaining the highest quality staff

--




Brendan J. Griffin
Director & Assoc. Professor in Electron Microscopy
Centre for Microscopy and Microanalysis (M010)
Director Western Australian Centre for Microscopy
Associate Director NANO-MNRF
President Australian Microbeam Analysis Society
The University of Western Australia
First floor, Physics Building,
35 Stirling Highway
CRAWLEY, WA, AUSTRALIA 6009
ph 61-8-6488-2739 fax 6488-1087
mobile 0409-104-096
bjg-at-cmm.uwa.edu.au
http://cmm.uwa.edu.au/

CRICOS Provider No. 00126G


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 13:57:44 2005



From: diller-at-stefan-diller.com (by way of MicroscopyListserver)
Date: Mon, 20 Jun 2005 13:57:02 -0500
Subject: [Microscopy] viaWWW: Old KEVEX detector on KEVEX DELTA 8000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (diller-at-stefan-diller.com) from http://microscopy.com/MLFormMail.html on Monday, June 20, 2005 at 09:55:20
---------------------------------------------------------------------------

Email: diller-at-stefan-diller.com
Name: Stefan Diller

Title-Subject: [Microscopy] [Filtered] Old KEVEX detector on KEVEX DELTA 8000

Question: Dear All,
maybe there is someone out there who can help me connect an old KEVEX EDS (seems to be Model 3801M?) detector with a Preamplifier Modell 2002 on my "new" KEVEX Delta 8000 unit.

I especially need the layouts of
1- the PREAMP1 connector on the rear of the KEVEX 8000 concerning voltage output and preamp output coming from the detector (see image at: http://www.stefan-diller.com/rem/Kevex1.jpg)
2- the POWER connector on the EDS detector and also the voltages needed for the preamp Modell 2002 in the detector
(see image at: http://www.stefan-diller.com/rem/Kevex2.jpg and http://www.stefan-diller.com/rem/Kevex3.jpg )
3- or the simple answer: Is it possible to use this detector on the KEVEX 8000?


Best regards,
Stefan Diller

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 14:11:37 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Mon, 20 Jun 2005 15:46:17 -0400
Subject: [Microscopy] Re: JB-4 (BMM recips)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All:

Sort of a way out idea but what about vapor impregnation by Cyanoacrylates
then sputter coat? Like Forensic scientists use to get fingerprints of
unusual places.

Cheers!

Al Coritz
Electron Microscopy Sciences
www.emsdiasum.com


----- Original Message -----
} From: "by way of Ask-A-Microscopist" {Gervais.Sawyer-at-bcuc.ac.uk}
To: {microscopy-at-microscopy.com}
Sent: Monday, June 20, 2005 9:16 AM

Group,
As Tom mentioned, mixtures of butyl and methyl and
methacrylate produce easy to section blocks that can serve a variety
of purposes. These mixtures predate epoxies for TEM use and they are
unstable in TEM beams. But the other side of that coin is most of the
resin can be extracted after sectioning with a 10 minute incubation
in aceteone. This provides excellent access for an antibody probe to
most of the section volume. So for light level immuno work, BMM is
great stuff. My innovation was to dissolve some DTT in the resin mix
which keeps those pesky free radicals from oxidizing the daylights
out of the sample.

My original paper on this is:
Baskin TI, Busby CH, Fowke LC. Sammut M, Gubler F (1992)
Improvements in immunostaining samples embedded in methacrylate:
Localization of microtubules and other antigens throughout developing
organs in plants of diverse taxa. Planta 187: 405 - 413.

And a few later findings in the methods section of this paper:

Baskin TI, Wilson JE (1997) Inhibitors of protein kinases and
phosphatases alter root morphology and disorganize cortical
microtubules. Plant Physiology 113: 493 - 502.

If anyone wants to try this, contact me off line and I can
provide a few extra resources for you.

Happy slicing,
Tobias


}
} I have cut 0.5 to 2 um thick JB-4 sections using a dry glass knife
} without a boat on many occasions. As the section starts to come off
} on the knife face, help it along with one of the tines of a fine
} forceps so that the section doesn't crumble up. Gently lift the cut
} section and drop in one motion on a drop of water. be sure to let go
} of the section before it touches the water or it will shrivel up
} against the forceps. Heat the section down on a hot plate as one
} would do for an Epon section. It works but is tedious. Furthermore,
} I would say the frustration is unnecessary since BMMA (butyl-methyl
} methacrylate/methyl methacrylate mixtures) are less expensive
} (generic ingredients), easier to cut (cuts on water filled boats),
} and can be deplasticized using acetone. We love BMMA and haven't
} used JB-4 ever since the esteemed Dr. Tobias Baskin told us about
} it. I don't have the reference to his paper but he monitors this
} listserver and perhaps will post it.
}
}
}
} At 01:04 PM 06/20/05, you wrote:
}
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 20:03:50 2005



From: Stacey.Andringa-at-uc.edu (by way of MicroscopyListserver)
Date: Mon, 20 Jun 2005 20:03:07 -0500
Subject: [Microscopy] viaWWW: Siemens IA Filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Monday, June 20, 2005 at 14:35:52
---------------------------------------------------------------------------

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I need to have filaments retipped for a Siemens IA. Can anyone recommend a vendor for this?
Thanks.

Stacey Andringa

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 20:05:09 2005



From: artcollc-at-emirates.net.ae (by way of Ask-A-Microscopist)
Date: Mon, 20 Jun 2005 20:04:26 -0500
Subject: [Microscopy] AskAMicroscopist: Oversized Petrographic Thin Section Coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (artcollc-at-emirates.net.ae) from on Monday, June 20, 2005 at 09:36:41
---------------------------------------------------------------------------

Email: artcollc-at-emirates.net.ae
Name: k. j. dhar

Organization: artco llc.

Education: Graduate College

Location: Dubai, United Arab Emirates.

Question: We are a marketing company, distributing (since 1989)all types of Material Testing equipment.

Currently, one of our customers (who is engaged in Oil production, refining, distribution) has set up a Petroleum Institute. They have a requirement for Oversized Petrographic Thin Section Coverslips, in two sizes 42mmx25mm and
74x49mm with 0.16mm thickness.

We seek your assistance/suggestions on the likely source from whom this can be ordered.

Thanking you in anticipation and with best regards,

Kiran Dhar


---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 20 21:09:52 2005



From: Barbara :      bfoster-at-mme1.com
Date: Mon, 20 Jun 2005 21:08:51 -0500
Subject: [Microscopy] Re: AskAMicroscopist: SEM image OF ash structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Gervais,

Some years ago, Buehler Instruments (Waukegan,IL), made a special device to mount delicate samples like this. It consisted of a specially constructed vacuum jar in which was mounted a small ladle that could pour epoxy into mounting cups. The specimen was put in the cups, the jar closed, the epoxy poured, then a vacuum drawn. The result: the epoxy penetrated the delicate structure and permitted microtoming.

I actually saw the ash on a cigar mounted this way, so think that it should be a good solution for your problem. You can probably access Buehler on the web. If not, contact me off line and I will see if I can find their contact info.

Good hunting!

Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call us at (972)954-8011.

At 08:16 AM 6/20/2005, Gervais.Sawyer-at-bcuc.ac.uk wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 21 02:45:26 2005



From: gillian.2.brown-at-gsk.com
Date: Tue, 21 Jun 2005 08:43:56 +0100
Subject: [Microscopy] Re: JB-4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
it depends on what microscopy you are actually doing, replies already in
are for TEM.
I am using a modified version of JB-4 (much less catalyst and 'B') for IHC
at the light microscope level and the resin does not need to be removed. I
cut 2micron sections on a dry knife (often the same one I trimmed on as
the resin is so soft) but I can go lower. No I can't do TEM off the same
block..
Refs: Casey et al. (1988) Am J Pathol;131:183-189
Britten et al. (1993) Biotech Histochem;68:271-280

Gill Brown
Histopathology Group
Asthma and Allergy Disease Biology
GlaxoSmithKline Medicines Research Centre,
UK




"Hong Yi" {hyi-at-emory.edu}
20-Jun-2005 19:04

To
" {microscopy-at-microscopy.com} {microscopy-at-microscopy.com} "
cc

Subject
JB-4








------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Can anyone tell me if JB-4 embedded sample can be sectioned on an
ultramicrotome (Leica Ultracut S), and what the appropriate thickness
should it be cut at? Thank you in advance.

HONg
Emory










From MicroscopyL-request-at-ns.microscopy.com Tue Jun 21 05:11:26 2005



From: Robert H. Olley :      hinmeigeng-at-hotmail.com
Date: Tue, 21 Jun 2005 10:10:42 +0000
Subject: [Microscopy] Philips SEM 515 available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have a Philips SEM 515, about 17 years old, available. The instrument
itself is in very good working order, apart from a small electrical problem
with the pumping system which we did not bother to have fixed since we moved
to a newer instrument.

Anyone interested?

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 21 12:22:27 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 21 Jun 2005 10:21:38 -0700
Subject: [Microscopy] Final Epitaph for film-Kodak Ends Production of B/W print paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



This announcement in Yahoo News:
http://news.yahoo.com/s/ap/20050615/ap_on_bi_ge/kodak_paper


John Mardinly
Intel





From MicroscopyL-request-at-ns.microscopy.com Tue Jun 21 17:49:44 2005



From: gardnere-at-wpunj.edu (by way of MicroscopyListserver)
Date: Tue, 21 Jun 2005 17:49:03 -0500
Subject: [Microscopy] viaWWW: Confocal microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (gardnere-at-wpunj.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, June 21, 2005 at 13:50:12
---------------------------------------------------------------------------

Email: gardnere-at-wpunj.edu
Name: Eileen Gardner

Organization: William Paterson University

Title-Subject: [Microscopy] [Filtered] MListserver: Confocal microscope

Question: I am the chair of a Biology department at a state university in NJ. I am planning on purchasing a confocal microscope which would be used for both teaching and research. Do you have any ideas as to which brands would be easiest to use and maintain? There will be no technician dedicated to this microscope. Thanks.

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 21 18:37:37 2005



From: Jay Campbell :      microtomy-at-gmail.com
Date: Tue, 21 Jun 2005 18:36:55 -0500
Subject: [Microscopy] TEM digital camera options

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
We are trying to find a workable solution for a problem that I
believe others may have run up against regarding digital image
acquisition. We have a Tecnai T12 microscope with a high resolution
bottom mount digital camera (Gatan 4K x 4K). The problem is due to
the slow frame rate and small field of view of the camera makes it
difficult to focus and capture low magnification survey shots. We
have considered
adding a side-mount CCD camera to complement the main camera but would
like to hear from other users to see what our options are. Does anyone
have experience with direct video rate cameras (side mounted) for the
purpose of locating and focusing areas of interest?

Another option would be to add a multiple image stitching software
to the current camera and merge several higher mag images. My
concern here is image/ specimen drift over the time these multiple
acquisitions are made. I work primarily with slot grids and support
film which tends to exacerbate such problems.

Thanks in advance,
Jay


Jay Campbell
Research Specialist
University of Wisconsin
Laboratory of Molecular Biology
R.M. Bock Labs
1525 Linden Drive
Madison, WI 53706
jmcampbe-at-wisc.edu
608 263 8481



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 21 18:43:21 2005



From: Gordon Vrololjak :      gvrdolja-at-nature.berkeley.edu
Date: Tue, 21 Jun 2005 16:42:31 -0700 (PDT)
Subject: [Microscopy] Re: viaWWW: Confocal microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'd say there should be a technician or your going to have some trouble
with getting it to work. Maybe appoint someone to take care of it and
train people. Go with zeiss, they have nice confocal systems that are
really easy to use and integrate well with the software for
control/imaging.
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Tue, 21 Jun 2005, by way of MicroscopyListserver wrote:

}
}
} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (gardnere-at-wpunj.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, June 21, 2005 at 13:50:12
} ---------------------------------------------------------------------------
}
} Email: gardnere-at-wpunj.edu
} Name: Eileen Gardner
}
} Organization: William Paterson University
}
} Title-Subject: [Microscopy] [Filtered] MListserver: Confocal microscope
}
} Question: I am the chair of a Biology department at a state university
} in NJ. I am planning on purchasing a confocal microscope which would be
} used for both teaching and research. Do you have any ideas as to which
} brands would be easiest to use and maintain? There will be no
} technician dedicated to this microscope. Thanks.
}
} ---------------------------------------------------------------------------
}


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 21 20:36:24 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 21 Jun 2005 18:36:18 -0700
Subject: [Microscopy] Re: TEM digital camera options

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jay
I would think, the good solution to you would be side-mounted camera with
optical coupling - they normally shows quite decent refresh rate. Perhaps,
it would be wise to use the same manufacturer for 100% compatibility with
existing camera. As for focusing problem - it's kind of strange. Gatan's
4x4 has view area compatible to the film. As for "slowness", yes, it's
slow. You need to use at least 4x (even 8x) binning in the "view" mode. I
do find that focusing with wobbler is extremely effective with my camera -
BioScan 600W - it's top-mounted, so comparison is not quite adequate. They
have "loupe" in DM, which is also helpful. Gatan also offered auto-focus
plug-in and as far as I remember it was working nicely at their
demonstration with 4x4 camera. DigitalMontage is also great - they used
beam shift to extend the view, so you could take pictures quite quickly,
like 3x3 or even 4x4 arrays. If you'll evaporate carbon on top of your
plastic film - it significantly reduces the drift. You may do it over the
sections (even better). Sergey

At 04:36 PM 6/21/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Tue Jun 21 20:48:31 2005



From: Joe Kulik :      juk12-at-psu.edu
Date: Tue, 21 Jun 2005 22:09:47 -0400
Subject: [Microscopy] TEM: Cone angle of LaB6 emitter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here are some thoughts from my own experience.

} From what I have heard, the optics of the major instruments are all
comparable. The choice of instrument, then, depends on ease of
operation (software), reliability, and service. There are some
technical details in the design of the spectral features that might
bear on the sensitivity of the instrument, though.

I would recommend that you think carefully about what you want to do
with this instrument. Do you want an upright or inverted microscope?
You should decide which wavelengths you need for the stains you are
likely to encounter, and make a similar analysis of the lenses that
you will need (long or short working distances,magnifications, etc)..
You should also decide if you need spectral capabilities on both the
excitation and emission detectors.

A confocal microscope is a significant instrument, with a complexity
not that different from the old electron microscopes. You can't just
walk in and use it without training. As Gordon suggested, you shoud
make sure that there is someone with responsibility for the
instrument. This person should be familiar enough to give
instruction and help to individual users.


The software is critical. It isn't that the different software sets
will give you different capabilities, but the ease of different
operations may vary. As a result, you will find that you may have a
different personal reaction to the interface. You, or the person who
will be in charge of the instrument, should sit down with each of the
models that you are considering, and work with the instrument for an
extended period --just looking at the instruments at a meeting, for
instance, will not work. You should also see whether the output
files can be read by 3d party software, such as Metamorph, or ImageJ,
or even Photoshop so that you don't tie up a microscope by doing
image processing with it.

In order to make a decision about the brand, it seems to me that,
given your support situation, you should investigate the service that
is available, and its cost. Service contracts can be quite
expensive, and I have heard anecdotes about the difficulty of service
with most of the manufacturers (although I have also heard some
praises for the same service organizations).

For more details on this, scan the archives of the confocal listserv:
CONFOCAL-at-LISTSERV.BUFFALO.EDU


Hope these musings are helpful. I can give you specific experiences
offlist.

Joel


Date sent: Tue, 21 Jun 2005 17:49:03 -0500
To: microscopy-at-microscopy.com
} From: gardnere-at-wpunj.edu (by way of MicroscopyListserver)

On our JEM-2010 we use a LaB6 emitter with a 90 degree cone and 15
micron tip.

We are considering using a smaller cone angle and/or smaller tip radius.

Does anyone have any comments concerning whether the supposed
improvement in brightness and coherence is worth the shortened lifetime?

_________________________________

Joseph Kulik
Research Associate
Materials Research Institute
The Pennsylvania State University
194 MRI Bldg
University Park, PA 16802

Telephone: 814-865-0344
Fax: 814-863-8561
e-mail: juk12-at-psu.edu
_________________________________




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 08:05:28 2005



From: xiaohutang-at-gmail.com (by way of MicroscopyListserver)
Date: Wed, 22 Jun 2005 08:04:46 -0500
Subject: [Microscopy] viaWWW: 3D image reconstruction software for stereo SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (xiaohutang-at-gmail.com) from http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, June 22, 2005 at 02:27:04
---------------------------------------------------------------------------

Email: xiaohutang-at-gmail.com
Name: Xiaohu Tang

Organization: TU Delft, the Netherlands

Title-Subject: [Microscopy] [Filtered] 3D image reconstruction software for stereo SEM

Question: Hello everyone,

I am looking for a 3D image reconstruction software for stereo SEM to measure the sample volume. It looks like the MeX from Alicona is the only choice so far but the price is too expensive. Could anybody give me an idea for a cheaper software or a better method to measure the volume by SEM? Thank you.

Xiaohu Tang
Microlab
CiTG of Delft Technology University
The Netherlands
x.tang-at-citg.tudelft.nl

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 09:11:18 2005



From: Phaedra McGuinness :      scanning-at-fams.org
Date: Wed, 22 Jun 2005 10:10:36 -0400
Subject: [Microscopy] SCANNING 2006 Tuesday April 25 through Thursday April 27 in Washington, D.C.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Just a quick calendar item and announcement regarding SCANNING 2006.
The 17th annual meeting on the scanning microscopies will take place
Tuesday, April 25 through Thursday, April 27 in Washington, D.C. The
Welcome Reception will take place on Tuesday, April 25 at the National
Press Club.

SPECIAL EVENT:
On Wednesday, April 26 there will be a program day at NIST.
Transportation will be provided. This is a joint program day included
in your SCANNING registration fee.

For more information:

SCANNING/FAMS
P.O. Box 485
Mahwah, NJ 07430-0485
Tel: (201) 818-1010
Fax: (201) 818-0086
e-mail: scanning-at-fams.org
Internet: www.scanning.org

See you at the meeting!


Best regards,


Phaedra McGuinness
Managing Editor
SCANNING, The Journal of Scanning Microscopies
P.O. Box 485
Mahwah, NJ 07430
Tel: (201) 818-1010 * Fax: (201) 818-0086 *email: scanning-at-fams.org *
Web: www.scanning.org




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 10:26:52 2005



From: Gordon Couger :      gcc-at-couger.com
Date: Wed, 22 Jun 2005 10:26:01 -0500
Subject: [Microscopy] Re: viaWWW: 3D image reconstruction software for stereo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Xiaohu
Have you looked at ImageJ with the needed plugins?

ImageJ's main page is http://rsb.info.nih.gov/ij/

3D Toolkit Plug ins
http://ij-plugins.sourceforge.net/ij-3D-toolkit.html

3D Toolkit is a set of plug ins for 3D and 2D operations on
images in Image/J. The first group of plug ins reads and writes
images in VTKand MetaImage format.
Metaimage Reader
MetaImage Writer
VTK Reader
VTK Writer
The second group of plugins are early prototypes of plugins for:
Morphological dilation (max)
Morphological erosion (min)
Connected threshold region growing
Auto volume clipping

This may be the same set of plug ins
http://ij-plugins.sourceforge.net/3d-toolkit/

A Google search for:
imagej 3d volume
brings over 600 hits and ImageJ and most plugins are free.

ImageJ is Java port with a great deal of enhancement of Sicon
Image that USA National Institute of Health had done and put in
the public domain. Primarily as health sciences imaging tool but
with the open source pugin, scrip an macro language many
disciplines are using it for many things. It is platform
independent running on any machine ruining Java, Unix, Solaris,
Windows, Mac, Mac OS-10, Linux and some more. For a P code
machine it amazingly fast. I would like to see it run on
computer that ran Java in native mode.

It is bit harder to use that Paint shop but new functionality is
available almost on a daily basis and the support from the folks
that write the plug ins is on the whole much much better than
any commercial product that doesn't cost a fortune for
maintenance to provide that kind of service. In many ways ImageJ
support is better than the best commercial service because you
work with the person that wrote the code. It is not uncommon for
someone on the plugin mailing list to help you modify a plugin
for you needs. There are no commercial vendors that can turn out
code this way.

I have worked for software companies as a programmer and letting
the customer work with the programmer is just not done. It uses
too much of the programmers time and the customer finds out more
than management wants them to know about the product. Because
the programmers don't pretend that the patched up out of date
software that most products are made out of are anything else
but what it is. Most of the time management doesn't have the
money to write new software from scratch or look at Cisco when
they tried to write a new operating system for their routers and
got in disagreement with the programmers and the programmers
quit and went into business as Juniper who is now Ciscoes'
biggest competitor in their high end most profitable business.

Source code is available for most plugins and ImageJ will
compile them giving you a complete tool for learning to write
plugins if you want to. Most of use will be content to play the
sorcerer's apprentice and charge a few lines to make a plugin
that is almost what we do just what we want and not become full
blown programmers. But that is very valuable option when you
need something slightly different than the person that developed
the plug in needed. A Google search for 'imagej plugin' brings
up almost 10,000 hits I am sure many of those are duplicates but
3,000 to 5,000 plugins is not an unreasonable estimate.

Best Regards
Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org

by way of MicroscopyListserver wrote:
}
} Email: xiaohutang-at-gmail.com
} Name: Xiaohu Tang
}
} Organization: TU Delft, the Netherlands
}
} Title-Subject: [Microscopy] [Filtered] 3D image
reconstruction software for stereo SEM
}
} Question: Hello everyone,
}
} I am looking for a 3D image reconstruction software for
stereo SEM to measure the sample volume. It looks like the MeX
from Alicona is the only choice so far but the price is too
expensive. Could anybody give me an idea for a cheaper software
or a better method to measure the volume by SEM? Thank you.
}
} Xiaohu Tang
} Microlab
} CiTG of Delft Technology University
} The Netherlands
} x.tang-at-citg.tudelft.nl
}



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 11:50:45 2005



From: Xiaohu Tang :      xiaohutang-at-gmail.com
Date: Wed, 22 Jun 2005 18:50:01 +0200
Subject: [Microscopy] Re: viaWWW: 3D image reconstruction software for stereo SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gordon, thank you for the valuable information. I checked ImageJ and
could not find the proper plugin for my problem: I want to use the SEM
stereo image pairs by tilting the sample and find out the volume data
from the 3D reconstruction. Based on my understanding (I am not so
familar with this area), most of the ImageJ plugins about 3D volume
depend on a series of image slices but not like my case. All the
suggestions and information will be highly appreciated.

Xiaohu Tang
Microlab
CiTG of Delft Technology University
The Netherlands
x.tang-at-citg.tudelft.nl

On 6/22/05, Gordon Couger {gcc-at-couger.com} wrote:
} Hi Xiaohu
} Have you looked at ImageJ with the needed plugins?
}
} ImageJ's main page is http://rsb.info.nih.gov/ij/
}
} 3D Toolkit Plug ins
} http://ij-plugins.sourceforge.net/ij-3D-toolkit.html
}
} 3D Toolkit is a set of plug ins for 3D and 2D operations on
} images in Image/J. The first group of plug ins reads and writes
} images in VTKand MetaImage format.
} Metaimage Reader
} MetaImage Writer
} VTK Reader
} VTK Writer
} The second group of plugins are early prototypes of plugins for:
} Morphological dilation (max)
} Morphological erosion (min)
} Connected threshold region growing
} Auto volume clipping
}
} This may be the same set of plug ins
} http://ij-plugins.sourceforge.net/3d-toolkit/
}
} A Google search for:
} imagej 3d volume
} brings over 600 hits and ImageJ and most plugins are free.
}
} ImageJ is Java port with a great deal of enhancement of Sicon
} Image that USA National Institute of Health had done and put in
} the public domain. Primarily as health sciences imaging tool but
} with the open source pugin, scrip an macro language many
} disciplines are using it for many things. It is platform
} independent running on any machine ruining Java, Unix, Solaris,
} Windows, Mac, Mac OS-10, Linux and some more. For a P code
} machine it amazingly fast. I would like to see it run on
} computer that ran Java in native mode.
}
} It is bit harder to use that Paint shop but new functionality is
} available almost on a daily basis and the support from the folks
} that write the plug ins is on the whole much much better than
} any commercial product that doesn't cost a fortune for
} maintenance to provide that kind of service. In many ways ImageJ
} support is better than the best commercial service because you
} work with the person that wrote the code. It is not uncommon for
} someone on the plugin mailing list to help you modify a plugin
} for you needs. There are no commercial vendors that can turn out
} code this way.
}
} I have worked for software companies as a programmer and letting
} the customer work with the programmer is just not done. It uses
} too much of the programmers time and the customer finds out more
} than management wants them to know about the product. Because
} the programmers don't pretend that the patched up out of date
} software that most products are made out of are anything else
} but what it is. Most of the time management doesn't have the
} money to write new software from scratch or look at Cisco when
} they tried to write a new operating system for their routers and
} got in disagreement with the programmers and the programmers
} quit and went into business as Juniper who is now Ciscoes'
} biggest competitor in their high end most profitable business.
}
} Source code is available for most plugins and ImageJ will
} compile them giving you a complete tool for learning to write
} plugins if you want to. Most of use will be content to play the
} sorcerer's apprentice and charge a few lines to make a plugin
} that is almost what we do just what we want and not become full
} blown programmers. But that is very valuable option when you
} need something slightly different than the person that developed
} the plug in needed. A Google search for 'imagej plugin' brings
} up almost 10,000 hits I am sure many of those are duplicates but
} 3,000 to 5,000 plugins is not an unreasonable estimate.
}
} Best Regards
} Gordon
} Gordon Couger
}
} I collect links on information related to light microscopes.
} www.couger.com/microscope/links/gclinks.html
} Please forward anything you think might be useful to others.
} Microscope Documentation is at www.science-info.org
}
} by way of MicroscopyListserver wrote:
} }
} } Email: xiaohutang-at-gmail.com
} } Name: Xiaohu Tang
} }
} } Organization: TU Delft, the Netherlands
} }
} } Title-Subject: [Microscopy] [Filtered] 3D image
} reconstruction software for stereo SEM
} }
} } Question: Hello everyone,
} }
} } I am looking for a 3D image reconstruction software for
} stereo SEM to measure the sample volume. It looks like the MeX
} from Alicona is the only choice so far but the price is too
} expensive. Could anybody give me an idea for a cheaper software
} or a better method to measure the volume by SEM? Thank you.
} }
} } Xiaohu Tang
} } Microlab
} } CiTG of Delft Technology University
} } The Netherlands
} } x.tang-at-citg.tudelft.nl
} }
}
}



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 12:56:11 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Wed, 22 Jun 2005 10:55:24 -0700
Subject: [Microscopy] TEM: Cone angle of LaB6 emitter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joe;
I have used 60 degree cone emitters with great success on a
2000FX (don't recall the tip radius) and 60 degree cones with 5 micron
radius on a Topcon 002B. They add a nice bit of crispness to the high
resolution image, although they are no substitute for field emission. As
for brightness; no problem. As for lifetime, they don't last quite as
long at a 90 degree, so if you are budget constrained that might be an
issue, but for us it was well worth the minor extra operational cost.
Six months was typical, for a TEM used a lot every day. Usually the
wehnelt needed cleaning once or twice during the life of the tip so
maintenance effort was about the same as a 90 degree. Unfortunately,
Denka, which was our preferred source for that tip on the Topcon, has
stopped manufacturing that particular source.

John Mardinly

This opinion is the opinion of the auther and does not represent the
opinion of Intel Corporation.

-----Original Message-----
} From: Joe Kulik [mailto:juk12-at-psu.edu]
Sent: Tuesday, June 21, 2005 7:10 PM
To: Microscopy-at-microscopy.com

On our JEM-2010 we use a LaB6 emitter with a 90 degree cone and 15
micron tip.

We are considering using a smaller cone angle and/or smaller tip radius.

Does anyone have any comments concerning whether the supposed
improvement in brightness and coherence is worth the shortened lifetime?

_________________________________

Joseph Kulik
Research Associate
Materials Research Institute
The Pennsylvania State University
194 MRI Bldg
University Park, PA 16802

Telephone: 814-865-0344
Fax: 814-863-8561
e-mail: juk12-at-psu.edu
_________________________________






From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 13:33:04 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Wed, 22 Jun 2005 14:53:33 -0400
Subject: [Microscopy] Re: viaWWW: Confocal microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I do not know just how much you need to lower the magnification but the
following may just help?

Raise the eucentric stage to its highest position (moving into focus when
the objective lens is weakened) which will reduce the magnification factor
of the objective lens. Possibly lowering the magnification by 10% or more?

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.comood



----- Original Message -----
} From: "Jay Campbell" {microtomy-at-gmail.com}
To: {Microscopy-at-microscopy.com}
Sent: Wednesday, June 22, 2005 12:36 AM

Before you choose an instrument I think you need to reconsider your "no
dedicated technician" approach. A complex piece of multi-user equipment
with no one person to supervise or police its use is a recipe for
diaster. It won't be much good for teaching or research if it is not
working properly. While you may have a service contract, service
engineers are usually spread to thinly to drop by every week for minor
adjustments that no one understands or has time to learn.

Geoff

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************


by way of MicroscopyListserver wrote:

} Email: gardnere-at-wpunj.edu
} Name: Eileen Gardner
}
} Organization: William Paterson University
}
} Title-Subject: [Microscopy] [Filtered] MListserver: Confocal microscope
}
} Question: I am the chair of a Biology department at a state university in NJ. I am planning on purchasing a confocal microscope which would be used for both teaching and research. Do you have any ideas as to which brands would be easiest to use and maintain? There will be no technician dedicated to this microscope. Thanks.
}
} ---------------------------------------------------------------------------
}
}




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 14:24:36 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 22 Jun 2005 12:24:32 -0700
Subject: [Microscopy] Re: TEM digitisation: digital plates vs digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kerrie
Digital plate story is discussed nearly every year at ListServer. I don't
want to repeat everything what was discussed (perhaps you could check
archives). From my prospective, digital plates has only one positive side -
the linearity is superior. It may be useful if you do a lot of diffraction
work. For biological samples if you satisfied with film's linearity, then
why you would use image (digital) plates? From practical point of view,
image plates is quite inconvenient: you need to use nearly identical
procedure of loading, unloading, pumping etc as for the film and you are
limited in the number of images you could produce (same as film - 50). You
have to load plates manually in the scanner. Most procedures should be
performed in the dark I believe. With digital camera you have less
resolution but you could generate practically unlimited amount of images (I
normally produce about 100 images in 40 min) and vacuum in the microscope
is great (because you don't have to vent the camera). Images available
immediately (you don't need to wait for scanning - few min per plate I
believe). So, I don't think, image plates much suited for biological
applications keeping in mind the price etc. A few words about
resolution. Somebody on ListServer calculate that single EM film contains
about 7 Gbyte of information - it's enormous amount of information, which
we never use 100%. We normally cropped our images and print them small
(look on the Science magazine pages!), so we dramatically reduce the
resolution on the final "product". Then, such "final product" becomes
comparable with what we have with digital cameras. Keep in mind that even
super-duper printers produce gray tones with resolution about 150 dpi (may
be 300 at the best). Personally, I was surprised to see that 3x3" images
from my BioScan 1Mpix camera printed by good inkjet printer with "gray
inks" practically undistinguishable for the real photo-print. The
disadvantage of such low-res images - you could not crop or enlarge them,
so you just took many pictures at different magnification. For big field
you could combine a few pictures quite easily. I hope it'll help. Have a
great day, Sergey

P.S. A new argument against image plates just cames to me: laser. They
used, I guess, similar laser as for phosphorimager. That laser has limited
life - something like 3 years and should be replaced then. Laser itself is
about $20K, so you need to keep expensive service contract or be ready to
invest in a new laser 2 or 3 year later... I think, CCD chip and phosphor
screen on digital cameras deteriorated as well- mine is still OK after 3
years of extensive use.

At 06:52 AM 6/20/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 15:58:01 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Wed, 22 Jun 2005 13:56:59 -0700
Subject: [Microscopy] Re: Re: viaWWW: 3D image reconstruction software for stereo SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jun 22, 2005, at 9:50 AM, Xiaohu Tang wrote:

} Gordon, thank you for the valuable information. I checked ImageJ and
} could not find the proper plugin for my problem: I want to use the SEM
} stereo image pairs by tilting the sample and find out the volume data
} from the 3D reconstruction. Based on my understanding (I am not so
} familar with this area), most of the ImageJ plugins about 3D volume
} depend on a series of image slices but not like my case. All the
} suggestions and information will be highly appreciated.
}
Dear Xiaohu.
If Sterecon is still out there, it may suit your needs. It was used
in Albany NY some years ago, and some of the people there may know
whether it is still available.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 16:24:07 2005



From: Rick A. Harris :      raharris-at-ucdavis.edu
Date: Wed, 22 Jun 2005 14:20:38 -0700
Subject: [Microscopy] Re: viaWWW: Confocal microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have to agree with Geoff and others on this list. I have been in this
business for over 25 years and I have never seen a situation where a
piece of equipment with the complexity of a confocal or an SEM or a TEM
or FIB or STM or you name it can be maintained at factory spec without a
dedicated support person to manage the device and train users. Over and
over again at this campus I have seen PIs win grants for equipment
without first having thought through the idea of dedicated support.
They will assume an overloaded tech or lab maanger can do it in their
"spare time". That won't work and eventually the machine will not be
capable of quality work or reliability. The equipement wll be used less
and less and within a short time will be salvaged out. Then, in a year
or three, another PI will need that same piece of equipment and we will
start the process again.

Rick Harris
Unniversity of California
Davis, CA



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 16:53:03 2005



From: Mike Bode :      Mike.Bode-at-soft-imaging.net
Date: Wed, 22 Jun 2005 15:51:04 -0600
Subject: [Microscopy] viaWWW: 3D image reconstruction software for stereo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello Xiaohu,

Stereo techniques usually give you a surface reconstruction, not a volume
reconstruction. Whether you can get meaningful volumetric data depends on
what you are looking at. For example: consider a spherical object lying on a
surface. Stereo images are usually taken at a few degrees tilt apart, so the
two images essentially see the same half of the sphere. You can measure half
of the sphere's volume, but since you cannot see the "backside", you have to
make assumptions. For a sphere, it is fairly easy, but if your object is
irregular, you may not be able to measure its volume without making
assumptions what the "hidden" part looks like.

I am sure you have considered this. I would also point you to our website,
which has information about the stereo module in our software:
http://www.soft-imaging.com/rd/english/405.htm

mike



Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: by way of MicroscopyListserver [mailto:xiaohutang-at-gmail.com]
Sent: Wednesday, June 22, 2005 07:05
To: microscopy-at-microscopy.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (xiaohutang-at-gmail.com) from
http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html on
Wednesday, June 22, 2005 at 02:27:04
---------------------------------------------------------------------------

Email: xiaohutang-at-gmail.com
Name: Xiaohu Tang

Organization: TU Delft, the Netherlands

Title-Subject: [Microscopy] [Filtered] 3D image reconstruction software for
stereo SEM

Question: Hello everyone,

I am looking for a 3D image reconstruction software for stereo SEM to
measure the sample volume. It looks like the MeX from Alicona is the only
choice so far but the price is too expensive. Could anybody give me an idea
for a cheaper software or a better method to measure the volume by SEM?
Thank you.

Xiaohu Tang
Microlab
CiTG of Delft Technology University
The Netherlands
x.tang-at-citg.tudelft.nl

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 16:57:45 2005



From: Ken Converse :      kenconverse-at-qualityimages.biz
Date: Wed, 22 Jun 2005 20:07:00 -0400
Subject: [Microscopy] Re: viaWWW: Confocal microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


-----Original Message-----
} From: "Rick A. Harris" {raharris-at-ucdavis.edu}

The perspective from the Field Service Engineer:

If I had to drop by every customer every week or so for minor "stuff", I
would be: 1) out of business (broke), 2) out of my mind 3) out of customers
because I can't visit all of them that often and the ones who had REAL
problems expect (rightly so) to be taken care of quickly and thoroughly.

PLEASE, PLEASE, PLEASE do not expect your field service engineer to
substitute for a good technician. It is unfair to ALL involved.

If you want your service organization to provide full-time service, expect
to pay 10 or 15 times what you're currently paying for your service
contract.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
} From: Geoff McAuliffe [mailto:mcauliff-at-umdnj.edu]
Sent: Wednesday, June 22, 2005 2:54 PM
To: by way of MicroscopyListserver
Cc: microscopy-at-microscopy.com

Before you choose an instrument I think you need to reconsider your "no
dedicated technician" approach. A complex piece of multi-user equipment
with no one person to supervise or police its use is a recipe for
diaster. It won't be much good for teaching or research if it is not
working properly. While you may have a service contract, service
engineers are usually spread to thinly to drop by every week for minor
adjustments that no one understands or has time to learn.

Geoff

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************


by way of MicroscopyListserver wrote:

} Email: gardnere-at-wpunj.edu
} Name: Eileen Gardner
}
} Organization: William Paterson University
}
} Title-Subject: [Microscopy] [Filtered] MListserver: Confocal microscope
}
} Question: I am the chair of a Biology department at a state university in
NJ. I am planning on purchasing a confocal microscope which would be used
for both teaching and research. Do you have any ideas as to which brands
would be easiest to use and maintain? There will be no technician dedicated
to this microscope. Thanks.
}
} ---------------------------------------------------------------------------
}
}






___________________________________________________________
$0 Web Hosting with up to 200MB web space, 1000 MB Transfer
10 Personalized POP and Web E-mail Accounts, and much more.
Signup at www.doteasy.com




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 21:27:40 2005



From: Qi Zhang :      qizhang-at-physics.unc.edu
Date: Wed, 22 Jun 2005 22:26:30 -0400
Subject: [Microscopy] JEM-2010F desiccator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We have been having oil leak problem with our JEM-2010F desiccator. We
have had 3 desiccators (EMDSC-U10A) and had leaking every 5-6 months. We
were told that the parts replacement wouldn't help so much, because this
model had design problem with about 40% failure possibility. Now, we are
looking for new reliable type of desiccator. Does anyone have any
suggestion? Thanks in advance!

With best regards,
********************************************************
Qi Zhang, Ph.D
164 Phillips Hall, CB#3255
Department of Physics and Astronomy
the University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
Tel: 919-843-2407 (Office)
919-843-0974 (EM Lab)
Fax: 919-962-0480
E-mail: qizhang-at-physics.unc.edu



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 22 22:12:14 2005



From: Matt Olszta :      mjo10-at-psu.edu
Date: Wed, 22 Jun 2005 23:09:56 -0400
Subject: [Microscopy] TEM-sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,
I am currently trying to make TEM samples out of very porous sintered
capacitor materials (Nb and NbO) in which I need to collect stoichiometric
data via EELS analysis. The problem in making these samples is that they
do not polish or ion mill well due to their porous morphology. I had tried
to infiltrate with M-bond, which helped considerably, but even after plasma
treating the samples before analysis, a large C-K edge remained throughout
the sample (which unfortunately sits right in between the elements of
interest). I was wondering if anyone had any suggestions of a filler that
could be used to infiltrate a porous body and then could be easily removed
post ion thinning. Any help would be greatly appreciated.

Regards
Matt Olszta Ph.D.
Materials Research Institute
Penn State University



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 01:02:01 2005



From: :      Ross.Hamilton-at-csl.com.au
Date: Thu, 23 Jun 2005 15:03:45 +1000
Subject: [Microscopy] TEM Negative Stains for Vaccine Formulations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We wish to negatively stain some vaccine formulations.
Does anyone know of a negative stain that you can use at acid pH that is not a uranyl salt?
Does anyone know of a negative stain that you can use at basic pH that is not sodium tetraborate? We have used sodium tetraborate but it tends to "bubble" under the beam!

Ross Hamilton,
Senior Scientist,
E.M. Unit,
Bioanalytical Sciences,
CSL Limited,
45 Poplar Road,
Parkville,3052
Victoria,Australia.
+61 3 9389 1307



****************************************************************************************************************
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and destroy all copies. Thank you.

CSL Limited A.C.N. 051 588 348
45 Poplar Road Parkville Victoria 3052 Australia
Phone: +61 3 9389 1911 Fax: +61 3 9389 1434
***************************************************************************************************************




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 07:35:37 2005



From: Bill Miller :      microbill-at-mohawk.net
Date: Thu, 23 Jun 2005 08:34:55 -0400
Subject: [Microscopy] Re: Re: TEM digitisation: digital plates vs digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sounds sort of like Pixel envy doesn't it? - since the new imaging
plate systems can make images with up to 110Mega pixels - you'd need
to shoot more than 100 pictures at 1Meg each to get equivalent
images - and by the way the systems use diode lasers that have very
long life and they don't cost anywhere near $20K


Imaging plates are not necessarily the right solution for every
situation but to categorically state that they are useless for
biological use is simply wrong. Like with any system there are
strengths and weaknesses but if nothing else the full field of view
pixels/$ is unbeatable.


Bill Miller

At 03:24 PM 6/22/2005, Sergey Ryazantsev wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 08:35:45 2005



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 23 Jun 2005 08:35:03 -0500
Subject: [Microscopy] EPON-what is is made of?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues...

Can someone tell me the nominal consitutents of EPON used
for embedding TEM biological samples. Specifically what
are it's major elemental components. (C, H, O, N, ......???)

It is not obvious to me which of the many EPONS listed in a Google search is
the one typically used by the life science community in
preparing TEM sections.



Thanks in advance,

Nestor
Your Friendly Neighborhood SysOp


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 08:35:51 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Thu, 23 Jun 2005 08:35:03 -0500
Subject: [Microscopy] Re: TEM Negative Stains for Vaccine Formulations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

check out www.nanoprobes.com they sell tungstate and vanadate based
negative stains. i have never used them so can't comment.

At 12:03 AM 06/23/05, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 08:45:07 2005



From: dwaugh-at-kent.edu (by way of MicroscopyListserver)
Date: Thu, 23 Jun 2005 08:44:21 -0500
Subject: [Microscopy] viaWWW: film for contact microradiographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dwaugh-at-kent.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, June 23, 2005 at 08:34:28
---------------------------------------------------------------------------

Email: dwaugh-at-kent.edu
Name: David Waugh

Organization: Kent State University, Geology

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: I'm looking to make contact microradiographs of crab claw thin sections (~40 microns thick). We have an x-ray machine, the problem is the film. Doing a google search I have found two Kodak films that are used, but can't figure out if they are still in production. Kodak SO-343, seems to be popular, I can't tell if it is an xray or holographic film, but either way there is no mention of it on the Kodak website or from any supplier, same thing for Kodak "spectroscopic film". A film "Fuji B&W POS/71337" that I found in one paper, does not seem to exist. I need something that is very high resolution, and preferable can be developed with standard B&W chemistry. Any ideas on what the current films in use are? Would a conventional film like Kodak T-max work (we have that film and chemical already lying around in the now mostly mothballed darkroom)? Thanks, David

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 08:55:09 2005



From: Mike Nesta :      mnesta-at-ebsciences.com
Date: Thu, 23 Jun 2005 09:54:27 -0400
Subject: [Microscopy] Re: viaWWW: Siemens IA Filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

At Energy Beam Sciences we can rebuild any tungsten filament, including
Siemens. We also provide new mounted filaments for most Microscopes. You
can contact us on (800) 992-9037 or by email at ebs-at-ebsciences.com.

Michael R. Nesta
Managing Director
Energy Beam Sciences, Inc.
Tel: 860 653-0411
Fax: 860 653-0422
MNesta-at-ebsciences.com
www.ebsciences.com
“ADDING BRILLIANCE TO YOUR VISION”



by way of MicroscopyListserver wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 09:08:42 2005



From: twigg-at-estd.nrl.navy.mil
Date: Thu, 23 Jun 2005 10:09:16 -0400
Subject: [Microscopy] Beam stop and TEM diffraction pattern recording by digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am posed to finally make the leap to a digital camera (a Gatan
Ultrascan 1000) after years of SO-163. One potential problem that I
face, however, is digital recording of SAD diffraction patterns. My
top-entry Hitachi H-9000UHR HRTEM did not come with a beam stop (that
rod or pointer thing that you use to block the main beam while
recording a diffraction pattern) and it is therefore my concern that
I might not be able to record diffraction patterns without damaging
the digital camera. Hitachi does not seam to have a beam stop thing
for my vintage (1991) H-9000, so I am behind the eight ball in this
regard. I hear from Gatan that I should try to avoid using film once
the Ultrascan is installed, so that that flaked-off film or whatever
does not fall onto the digital camera and mess it up, so using film
to record diffraction patterns may not be an option either. Any
suggestions?

Thanks,

Mark
--
Dr. Mark E. Twigg
Code 6812
Naval Research Laboratory
4555 Overlook Ave., S.W.
Washington DC 20375

tel: (202) 404-8543
fax: (202) 404-7194


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 09:18:16 2005



From: Tom Phillips :      phillipst-at-missouri.edu
Date: Thu, 23 Jun 2005 09:17:27 -0500
Subject: [Microscopy] Re: EPON-what is is made of?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Epon 812 was a Shell patented formulation and has now been replaced by
numerous "identical chemical equivalents" according to EM suppliers but
rarely are the formulations given. It is a

glycerol polyglycidyl ether (i think it is a mixture of different glycidyl
ethers).

"Epon resins" in lab formulations usually also contain NMA (Nadic©Methyl
Anhydride
(Methyl-5-Norbornene-2,3-Dicarboxylic Anhydride) as a hardner

C10H10O3

and

DDSA (Dodecenyl Succinic Anhydride) (about 2:1:1 for epon/nma/ddsa but this
varies in different labs) plus either DMP-30
Tri-(dimethylaminomethyl) phenol

C15H29N8OH or BDMA as catalysts

Tom


At 08:35 AM 06/23/05, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu





From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 09:20:25 2005



From: Mike Nesta :      mnesta-at-ebsciences.com
Date: Thu, 23 Jun 2005 10:19:39 -0400
Subject: [Microscopy] Re: RE: TEM: Cone angle of LaB6 emitter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear John,

The discontinued Denka source you are referring to was the M-7, which
featured a hard mounted tip. All of the tip configurations that were
available on the M-7 are still available on the M-3, wire mounted tip,
including the 60 degree cone angle/5 micron radius configuration you
referred to. The reason Denka discontinued the M-7 was that they
discovered through internal testing that the M-3 provides stability
equal to or greater than the M-7. Denka feels that this discovery
rendered the higher cost for the M-7 an unnecessary expense.

Disclaimer - Energy beam Sciences, Inc. is the US distributor for Denka
LaB6 and Thermal Field Emission Cathodes.

Michael R. Nesta
Managing Director
Energy Beam Sciences, Inc.
Tel: 860 653-0411
Fax: 860 653-0422
MNesta-at-ebsciences.com
www.ebsciences.com
“ADDING BRILLIANCE TO YOUR VISION”





Mardinly, John wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 10:00:02 2005



From: Sinkler, Wharton :      Wharton.Sinkler-at-uop.com
Date: Thu, 23 Jun 2005 09:59:24 -0500
Subject: [Microscopy] Beam stop and TEM diffraction pattern recording by

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mark,

I routinely use our US-1000 for single crystal diffraction patterns with
no beam stop. This is because the central beam is actually fairly weak
in this situation, as so much intensity is getting diffracted. If this
is the large majority of your diffraction work you will be fine.

On the other hand, when collecting polycrystalline ring patterns or
patterns from amorphous samples, you will have a problem. The rings are
typically weak and the central spot is strong. To get decent signal to
noise in the rings you need to up the exposure time or dose. This is
where I use the beam stop so if you do a lot of this you should consider
making one. Without the stop you'll pretty heavily saturate the CCD,
producing a large streak across the central spot.

Talk to Gatan about conditions which would damage the phosphor on the
CCD, but I think they are rather extreme - typically these are pretty
robust for normal TEM and diffraction conditions.

Regards,
Wharton

*************************************************************
Wharton Sinkler, PhD.
UOP LLC
50 E. Algonquin Rd.
Des Plaines IL 60017-5017
mailto: wharton.sinkler-at-uop.com
tel 847-391-3878
fax 847-391-3719



-----Original Message-----
} From: twigg-at-estd.nrl.navy.mil [mailto:twigg-at-estd.nrl.navy.mil]
Sent: Thursday, June 23, 2005 9:09 AM
To: Microscopy-at-microscopy.com

I am posed to finally make the leap to a digital camera (a Gatan
Ultrascan 1000) after years of SO-163. One potential problem that I
face, however, is digital recording of SAD diffraction patterns. My
top-entry Hitachi H-9000UHR HRTEM did not come with a beam stop (that
rod or pointer thing that you use to block the main beam while
recording a diffraction pattern) and it is therefore my concern that
I might not be able to record diffraction patterns without damaging
the digital camera. Hitachi does not seam to have a beam stop thing
for my vintage (1991) H-9000, so I am behind the eight ball in this
regard. I hear from Gatan that I should try to avoid using film once
the Ultrascan is installed, so that that flaked-off film or whatever
does not fall onto the digital camera and mess it up, so using film
to record diffraction patterns may not be an option either. Any
suggestions?

Thanks,

Mark
--
Dr. Mark E. Twigg
Code 6812
Naval Research Laboratory
4555 Overlook Ave., S.W.
Washington DC 20375

tel: (202) 404-8543
fax: (202) 404-7194




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 10:18:22 2005



From: Technik :      technik-at-ditabis.de
Date: Thu, 23 Jun 2005 17:18:32 +0200
Subject: [Microscopy] Re: TEM digitisation: digital plates vs digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Community ,

I think the idea of the list server is to share experience one has made
in any topic
with the microscopy community. But this assumes that one has made this
experience personally.
Probably everybody believes and guesses a lot about instruments,
companies and persons but shall such
guesswork be shared on such a public experience based forum like this?

Please let me point out what up-to-date laser technology means:
Sergey is right, former laser had a limited life time. Such gas lasers
being produced some decades ago had this limitations
whereas modern semiconductor lasers as used in the DITABIS MICRON are
very stable and have a life time comparable to CCD sensors.
I am disappointed to see it obviously has become more and more common to
read "personal opinions" of some forum members
not being based on gained knowledge.

The resolution discussion: why does anybody want to have high resolution
systems when downsizing the
resolution and more important loosing the information which should be
the aim of any scientifical work.
I know that our scientific customers do need the information and don't
make high resolution images just for their personal satisfaction.

Another point mentioned by John Mardinly was that the imaging plate
store only electrons.
In principle he is right except that you can detect nearly every high
energy radiation with imaging plates.
With former imaging plate systems an identification of the imaging plate
- and thus the exposure - was impossible.
The Ditabis imaging plate system is able to optically scan the surface
(e.g. labeled with unique numbers.....) in parallel
to normal image scans. That label information is stamped into the final
image in one and the same run.
A software package even allows to merge TEM settings information into
the header of each respective image.

Best regards,
Andreas Elkeries

Dipl.-Ing.
Customer Support / R&D

-------------------------------------
DITABIS AG
Digital Biomedical Imaging Systems AG
Stuttgarter Str. 13
D-75179 Pforzheim, Germany

Tel.: +49 (0)7231 1559893
Fax.: +49 (0)7231 589791

a.elkeries-at-ditabis.de
http://www.ditabis.de
=====================================



} Sergey Ryazantsev wrote:
}
} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} Kerrie
} Digital plate story is discussed nearly every year at ListServer. I
} don't want to repeat everything what was discussed (perhaps you could
} check archives). From my prospective, digital plates has only one
} positive side - the linearity is superior. It may be useful if you do
} a lot of diffraction work. For biological samples if you satisfied
} with film's linearity, then why you would use image (digital) plates?
} From practical point of view, image plates is quite inconvenient: you
} need to use nearly identical procedure of loading, unloading, pumping
} etc as for the film and you are limited in the number of images you
} could produce (same as film - 50). You have to load plates manually in
} the scanner. Most procedures should be performed in the dark I
} believe. With digital camera you have less resolution but you could
} generate practically unlimited amount of images (I normally produce
} about 100 images in 40 min) and vacuum in the microscope is great
} (because you don't have to vent the camera). Images available
} immediately (you don't need to wait for scanning - few min per plate I
} believe). So, I don't think, image plates much suited for biological
} applications keeping in mind the price etc. A few words about
} resolution. Somebody on ListServer calculate that single EM film
} contains about 7 Gbyte of information - it's enormous amount of
} information, which we never use 100%. We normally cropped our images
} and print them small (look on the Science magazine pages!), so we
} dramatically reduce the resolution on the final "product". Then, such
} "final product" becomes comparable with what we have with digital
} cameras. Keep in mind that even super-duper printers produce gray
} tones with resolution about 150 dpi (may be 300 at the best).
} Personally, I was surprised to see that 3x3" images from my BioScan
} 1Mpix camera printed by good inkjet printer with "gray inks"
} practically undistinguishable for the real photo-print. The
} disadvantage of such low-res images - you could not crop or enlarge
} them, so you just took many pictures at different magnification. For
} big field you could combine a few pictures quite easily. I hope it'll
} help. Have a great day, Sergey
}
} P.S. A new argument against image plates just cames to me: laser.
} They used, I guess, similar laser as for phosphorimager. That laser
} has limited life - something like 3 years and should be replaced
} then. Laser itself is about $20K, so you need to keep expensive
} service contract or be ready to invest in a new laser 2 or 3 year
} later... I think, CCD chip and phosphor screen on digital cameras
} deteriorated as well- mine is still OK after 3 years of extensive use.
}
} At 06:52 AM 6/20/2005, you wrote:
}
}
} } ------------------------------------------------------------------------------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -------------------------------------------------------------------------------
} }
} }
} } I have been given the unenviable task of having to decide between a
} } digital
} } camera system and a digital imaging plate system (the Ditabis system) to
} } enable our older TEMs to become fully digital imaging systems. We
} } have Jeol
} } 1200EX and 100CX instruments and a Phillips CM10. Has anyone had
} } experience
} } of the Ditabis system? In the UK there is only one currently in use,
} } and I
} } have contact with that person. Our samples are biological samples, some
} } routine clinical specimens, but also research samples and cell culture
} } samples. We currently use Kodak em film 4489 and require the
} } replacement
} } system to have comparable resolution.
} } Any comments would be gratefully received.
}
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} 10833 Le Conte Ave, Room 33-080
} Los Angeles, CA 90095
}
} Phone: (310) 825-1144 (office)
} (310) 206-1029 (Lab)
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 11:11:34 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Thu, 23 Jun 2005 09:10:46 -0700
Subject: [Microscopy] Re: RE: TEM: Cone angle of LaB6 emitter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Michael;
Thank you for this good news. When I attempted to order an M7 a
few months ago from another vendor of Denka emitters, that information
was not available.

John Mardinly

-----Original Message-----
} From: Mike Nesta [mailto:mnesta-at-ebsciences.com]
Sent: Thursday, June 23, 2005 7:20 AM
To: Mardinly, John
Cc: Joe Kulik; Microscopy-at-microscopy.com




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 11:18:08 2005



From: :      nyilmaz-at-mersin.edu.tr
Date: Thu, 23 Jun 2005 19:17:26 +0300
Subject: [Microscopy] bacteria and bone marrow processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everybody

We're planning to study bacteria suspension (Staphylococcus aureus) and bone marrow with TEM. Does anybody help us for bacteria and bone marrow (about 1 ml) processing protocol for TEM?

Thanks...

Dr. Necat Yilmaz








From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 11:34:31 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Thu, 23 Jun 2005 09:33:16 -0700
Subject: [Microscopy] Re: TEM digitisation: digital plates vs digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Andreas Elkeries;
As Nestor has pointed out, it is inappropriate to make
disparaging remarks about individuals in the MSA Listserver, especially
as to whether their comments come from personal experience or
hallucination. It is especially surprising to see such obstreperousness
coming from a member of any 'Customer Support' staff. In my particular
case, I conducted an evaluation of Ditabis imaging plates through an
authorized US dealer of your product, as Mr. Miller can attest to. The
full results of that evaluation cannot be shared on the Listserver, but
I can say that no microscope data that would normally be printed on film
was present on the images I received, and in discussions with Mr.
Miller, there was never communicated any expectation that the microscope
data could ever be imprinted on imaging plates. If there is a new
development that would allow that, I think we would all be interested in
hearing more details about it.

John Mardinly


-----Original Message-----
} From: Technik [mailto:technik-at-ditabis.de]
Sent: Thursday, June 23, 2005 8:19 AM
To: Microscopy-at-microscopy.com; sryazant-at-ucla.edu

Dear Community ,

I think the idea of the list server is to share experience one has made
in any topic
with the microscopy community. But this assumes that one has made this
experience personally.
Probably everybody believes and guesses a lot about instruments,
companies and persons but shall such
guesswork be shared on such a public experience based forum like this?

Please let me point out what up-to-date laser technology means:
Sergey is right, former laser had a limited life time. Such gas lasers
being produced some decades ago had this limitations
whereas modern semiconductor lasers as used in the DITABIS MICRON are
very stable and have a life time comparable to CCD sensors.
I am disappointed to see it obviously has become more and more common to

read "personal opinions" of some forum members
not being based on gained knowledge.

The resolution discussion: why does anybody want to have high resolution

systems when downsizing the
resolution and more important loosing the information which should be
the aim of any scientifical work.
I know that our scientific customers do need the information and don't
make high resolution images just for their personal satisfaction.

Another point mentioned by John Mardinly was that the imaging plate
store only electrons.
In principle he is right except that you can detect nearly every high
energy radiation with imaging plates.
With former imaging plate systems an identification of the imaging plate

- and thus the exposure - was impossible.
The Ditabis imaging plate system is able to optically scan the surface
(e.g. labeled with unique numbers.....) in parallel
to normal image scans. That label information is stamped into the final
image in one and the same run.
A software package even allows to merge TEM settings information into
the header of each respective image.

Best regards,
Andreas Elkeries

Dipl.-Ing.
Customer Support / R&D

-------------------------------------
DITABIS AG
Digital Biomedical Imaging Systems AG
Stuttgarter Str. 13
D-75179 Pforzheim, Germany

Tel.: +49 (0)7231 1559893
Fax.: +49 (0)7231 589791

a.elkeries-at-ditabis.de
http://www.ditabis.de
=====================================



} Sergey Ryazantsev wrote:
}
}
------------------------------------------------------------------------
------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
-------
}
}
} Kerrie
} Digital plate story is discussed nearly every year at ListServer. I
} don't want to repeat everything what was discussed (perhaps you could
} check archives). From my prospective, digital plates has only one
} positive side - the linearity is superior. It may be useful if you do
} a lot of diffraction work. For biological samples if you satisfied
} with film's linearity, then why you would use image (digital) plates?
} From practical point of view, image plates is quite inconvenient: you
} need to use nearly identical procedure of loading, unloading, pumping
} etc as for the film and you are limited in the number of images you
} could produce (same as film - 50). You have to load plates manually in

} the scanner. Most procedures should be performed in the dark I
} believe. With digital camera you have less resolution but you could
} generate practically unlimited amount of images (I normally produce
} about 100 images in 40 min) and vacuum in the microscope is great
} (because you don't have to vent the camera). Images available
} immediately (you don't need to wait for scanning - few min per plate I

} believe). So, I don't think, image plates much suited for biological
} applications keeping in mind the price etc. A few words about
} resolution. Somebody on ListServer calculate that single EM film
} contains about 7 Gbyte of information - it's enormous amount of
} information, which we never use 100%. We normally cropped our images
} and print them small (look on the Science magazine pages!), so we
} dramatically reduce the resolution on the final "product". Then, such

} "final product" becomes comparable with what we have with digital
} cameras. Keep in mind that even super-duper printers produce gray
} tones with resolution about 150 dpi (may be 300 at the best).
} Personally, I was surprised to see that 3x3" images from my BioScan
} 1Mpix camera printed by good inkjet printer with "gray inks"
} practically undistinguishable for the real photo-print. The
} disadvantage of such low-res images - you could not crop or enlarge
} them, so you just took many pictures at different magnification. For
} big field you could combine a few pictures quite easily. I hope it'll

} help. Have a great day, Sergey
}
} P.S. A new argument against image plates just cames to me: laser.
} They used, I guess, similar laser as for phosphorimager. That laser
} has limited life - something like 3 years and should be replaced
} then. Laser itself is about $20K, so you need to keep expensive
} service contract or be ready to invest in a new laser 2 or 3 year
} later... I think, CCD chip and phosphor screen on digital cameras
} deteriorated as well- mine is still OK after 3 years of extensive use.
}
} At 06:52 AM 6/20/2005, you wrote:
}
}
} }
------------------------------------------------------------------------
------
} }
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
------------------------------------------------------------------------
-------
} }
} }
} } I have been given the unenviable task of having to decide between a
} } digital
} } camera system and a digital imaging plate system (the Ditabis system)
to
} } enable our older TEMs to become fully digital imaging systems. We
} } have Jeol
} } 1200EX and 100CX instruments and a Phillips CM10. Has anyone had
} } experience
} } of the Ditabis system? In the UK there is only one currently in use,

} } and I
} } have contact with that person. Our samples are biological samples,
some
} } routine clinical specimens, but also research samples and cell
culture
} } samples. We currently use Kodak em film 4489 and require the
} } replacement
} } system to have comparable resolution.
} } Any comments would be gratefully received.
}
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} 10833 Le Conte Ave, Room 33-080
} Los Angeles, CA 90095
}
} Phone: (310) 825-1144 (office)
} (310) 206-1029 (Lab)
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
}
}
}
}
}





From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 12:25:31 2005



From: Gilles Grondin :      gilles.grondin-at-usherbrooke.ca
Date: Thu, 23 Jun 2005 13:21:34 -0400
Subject: [Microscopy] Fwd: petit contrat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} From: gilles_grondin-at-uqtr.ca
}
} *************************************************************
} Cutting sections from methacrylate-embedded blocks (non-plastination)
}
} Hi,
} Anyone want to cut some plastic sections for us?
}
} We have a number of tissue blocks embedded for light microscopy in hpma, and
} we would like them sectioned at 1.5 - 2 microns, and mounted on slides. We
} aren't currently set up for cutting plastic sections, and if there is anyone
} with some spare microtome capacity who would like to earn some pocket money
} here's your chance. I would like an estimate of the per-slide cost of cutting
} sections and mounting them on a glass slide. Just guessing, initially we
} would
} like about 100 sections from about 20 different blocks.
}
} As an aside, anyone tried to embed blocks with P40 and then cut them for
} light
} microscopy?
}
} Sincerely,
} Dave Griffiths
}
} David Griffiths
} Section of Anatomy and Pathology
} Norwegian School of Veterinary Science
} P. O. Box 8146 Dep
} N-0033 Oslo
} Norway
} Tel: 22964542/Fax: 22964764
} E-mail: david.griffiths-at-veths.no
} ****************************************************
}
} -------------------------------------------------
} Courriel expédié via https://courriel.uqtr.ca




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 18:56:17 2005



From: Bill Miller :      microbill-at-mohawk.net
Date: Thu, 23 Jun 2005 19:55:36 -0400
Subject: [Microscopy] digital imaging plate tracking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

As rightly pointed out by John Mardinly, a drawback to the imaging
plates has always been the lack of foolproof data and image
correlation. There is now a solution that for some TEMs will
automatically read the microscope parameters and link it with bar
coded plates which are read during the scan process.

A complete description of the tracking system is available though ElectroImage.

Best Regards - Bill Miller




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 23 20:18:28 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 23 Jun 2005 18:15:48 -0700
Subject: [Microscopy] Re: Re: TEM digitisation: digital plates vs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bill
This is very common mistake thinking that more pixels gives you 'more'
resolution. If your sample has, let say 10 nm details and nothing smaller,
I would expect that quite decent 'resolution' on this particular sample
would be if you utilize something like 3 pixels per 10 nm =} 3.3
nm/pixel. You simply don't need more pixels if there is nothing smaller on
your sample. Adding more pixels you will basically add more noise to the
image and that's it. Ultrathin sections of biological material, which I
called 'biological samples' have quite poor resolution because of fixation,
dehydratation plastic embedding and so on. Therefore, you will just spoil
those 110M of pixels... It's like some people expected 3A resolution
(resolution of the scope itself) on 80 nm thick poorly infiltrated
chattered plastic sections of biological material, no way: 100A may be...

Again, even on my 3.3GHz Pentium 5 with 2 Gb 3400 RAM computer, I do see
deterioration in performance if I am working on couple 50Mb pictures, so
you really need super computer to handle your 110 Mb pictures. And I am
not talking about printing those images: on my networked color Xerox Phaser
7700GX with a lot of memory (maximum permitted) it takes about 20-30 sec to
sent 10-20 Mb image (and more than minute to print at 1200 dpi). Your files
will simply kill all our UCLA network! I think, you guys have better
network, probably dedicated to handle such giant files.... So, I am just
reasoning, why from my prospective, there is not much use of image plates
in biological applications. I am glad that Ditabis is using solid state
lasers - it definitely will add some juice to the system. By the way, how
long it takes to scan one plate at maximum resolution?

I do belive that image plate technology has a great potential in material
science applications when huge dynamic range is critical. Have a great
day, Sergey

At 05:34 AM 6/23/2005, you wrote:
} Sounds sort of like Pixel envy doesn't it? - since the new imaging plate
} systems can make images with up to 110Mega pixels - you'd need to shoot
} more than 100 pictures at 1Meg each to get equivalent images - and by the
} way the systems use diode lasers that have very long life and they don't
} cost anywhere near $20K
}
}
} Imaging plates are not necessarily the right solution for every situation
} but to categorically state that they are useless for biological use is
} simply wrong. Like with any system there are strengths and weaknesses but
} if nothing else the full field of view pixels/$ is unbeatable.
}
}
} Bill Miller
}
} At 03:24 PM 6/22/2005, Sergey Ryazantsev wrote:
}
}
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Fri Jun 24 02:44:29 2005



From: Bill Miller :      microbill-at-mohawk.net
Date: Fri, 24 Jun 2005 10:15:05 -0400
Subject: [Microscopy] Re: Re: Re: TEM digitisation: digital plates vs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
.. I might add for those interested in getting data from a Philips EM 400,
410, 420 and 430 that there is a interface card you can install without much
knowledge.
It will get you
High tension value
Magnification / diffraction camera length (dependent on chosen camera: TV,
sheet film, 224x36mm port)
Filmcode
Filmnumber
Exposure time on filmilm
Please see http://www.stefan-diller.com/rem_tem3_en.htm.

For Zeiss EM 10 A, B, C there is the same possibility but without
transmission of filmnumbers (but I might develop such an interface if there
is the need).
Values are only
High tension
Magnification
0.4x Magnifying switch
Please see http://www.stefan-diller.com/rem_tem4_en.htm
Installation should be by service technician.

Values are send via RS 232 interface to the computer. There should be no
problem to incorporate them in any imaging software.

Best regards,
Stefan Diller



----- Original Message -----
} From: "Bill Miller" {microbill-at-mohawk.net}
To: {Microscopy-at-microscopy.com}
Sent: Friday, June 24, 2005 1:55 AM

Hello Mark,

Diffraction beam stop is a standard option of SIA TEM CCD cameras. It is
available separately (without a camera) as well.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
} From: {twigg-at-estd.nrl.navy.mil}
To: {Microscopy-at-microscopy.com}
Sent: Thursday, June 23, 2005 10:09 AM

Sergey - I would normally ignore this kind of
thing but you have missed the point entirely and
I hope this helps to clarify it. The number of
pixels does not necessarily define the resolution
of an image - in this case it defines the field
of view. The pixels can be as small as 7.5um in
the image plane but what counts is that the image
can be as large as ~11,000 x 10,000 pixels. The
high pixel count allowing you to take MANY fewer
pictures to get the same information; so one
picture contains the information (pixels) of 100
1mega pixel images (if both sensors have 7.5um
pixel in the image plane) - I've responded to
your points individually below..

Bill



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Bill
This is very common mistake thinking that more
pixels gives you 'more' resolution.

} No one ever said that more pixels gave
more resolution - resolution is dependent upon
the pixel size in the image plane as well as
point spread and MTF of the system - more pixels
give a larger field of view not more resolution.

If your sample has, let say 10 nm details and
nothing smaller, I would expect that quite decent
'resolution' on this particular sample would be
if you utilize something like 3 pixels per 10 nm
=} 3.3 nm/pixel. You simply don't need more
pixels if there is nothing smaller on your sample.

} Resolution is one thing but being able to
actually visualize a structure requires
significant oversampling - how confident would
you be of a structure represented by 3 pixels -
would you ever enlarge the image enough to even
really see 3 pixels? The Nyquist sampling limit
is fine for mathematically determining the
resolution limit of an image but over sampling by
a factor of 5 or 10 will give you enough pixels
to actually see the structure. But resolution is
not the relavent point to all of this , it is field of view.

Adding more pixels you will basically add more
noise to the image and that's it.

} More pixels = more noise – an interesting, but
an obviously flawed concept or there would be NO
market for high end digital cameras of any
sort. It might be true if the pixels were
smaller rather than spread over a larger
area. In this case the idea of more pixels is to
expand the field of view not to increase the
resolution. The pixel size determines the
resolution not the pixel number - you are confusing the two.

Ultrathin sections of biological material, which
I called 'biological samples' have quite poor
resolution because of fixation, dehydratation
plastic embedding and so on. Therefore, you will
just spoil those 110M of pixels... It's like
some people expected 3A resolution (resolution of
the scope itself) on 80 nm thick poorly
infiltrated chattered plastic sections of
biological material, no way: 100A may be...

} So with more pixels you can get the 100A
resolution in an image at lower magnification and
a concomitantly larger field of view - again
fewer pictures to shoot -- seems like simple enough concept

Again, even on my 3.3GHz Pentium 5 with 2 Gb 3400
RAM computer, I do see deterioration in
performance if I am working on couple 50Mb
pictures, so you really need super computer to
handle your 110 Mb pictures. So is it easier and
faster to process over 100 images or a single
image that does not have to be stitches to get
all of the information? And I am not talking
about printing those images: on my networked
color Xerox Phaser 7700GX with a lot of memory
(maximum permitted) it takes about 20-30 sec to
sent 10-20 Mb image (and more than minute to print at 1200 dpi).

} Are the people who are scanning their negatives
doing so at 300dpi for fear thet the file would
otherwise be too big? Absolutely not. People pay
a great deal of money for scanners with 7um
pixels so that they get as much information as
possible form their TEM negatives. Big files are
a fact of life in science - how big is a
confocal image made of 100’s of serial images?

simply kill all our UCLA network!

} So don't use it, but that doesn't mean that
someone else might not "feel" like you do.

I think, you guys have better network, probably
dedicated to handle such giant files.... So, I
am just reasoning, why from my prospective, there
is not much use of image plates in biological applications.

} That’s like asking why everybody doesn't drive
a Ferrari!!! And just what direct knowledge of
the use of plates in biology do you have? Just
like with megapixel CCD cameras, price, not
performance is the bigger barrier. How many
people do you think are stuck with a 1meg small
field of view camera for biological work - forced
to shoot many hundreds of images to get a field
of view comparable in size and resolution to what
they have been getting with film - just because
they couldn't afford the 16meg CCD camera? Would
you have bought the 1meg camera if you could have
afforded a 4 or 16meg one? So, I wonder why there
is not much use of 8k x 8k cameras in biological
applications? Increased noise? Nope, it's the Money!

I am glad that Ditabis is using solid state
lasers - it definitely will add some juice to the
system. By the way, how long it takes to scan one plate at maximum resolution?

} a few unattended minutes - far less time than
it takes to shoot and stitch the comparable 100+
1 meg images required to cover the same field of view.

I do belive that image plate technology has a
great potential in material science applications
when huge dynamic range is critical. Have a great day, Sergey






At 05:34 AM 6/23/2005, you wrote:

Sounds sort of like Pixel envy doesn't it? -
since the new imaging plate systems can make
images with up to 110Mega pixels - you'd need to
shoot more than 100 pictures at 1Meg each to get
equivalent images - and by the way the systems
use diode lasers that have very long life and
they don't cost anywhere near $20K


Imaging plates are not necessarily the right
solution for every situation but to categorically
state that they are useless for biological use is
simply wrong. Like with any system there are
strengths and weaknesses but if nothing else the
full field of view pixels/$ is unbeatable.


Bill Miller

At 03:24 PM 6/22/2005, Sergey Ryazantsev wrote:



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Kerrie
Digital plate story is discussed nearly every
year at ListServer. I don't want to repeat
everything what was discussed (perhaps you could
check archives). From my prospective, digital
plates has only one positive side - the linearity
is superior. It may be useful if you do a lot of
diffraction work. For biological samples if you
satisfied with film's linearity, then why you
would use image (digital) plates? From practical
point of view, image plates is quite
inconvenient: you need to use nearly identical
procedure of loading, unloading, pumping etc as
for the film and you are limited in the number of
images you could produce (same as film - 50). You
have to load plates manually in the
scanner. Most procedures should be performed in
the dark I believe. With digital camera you have
less resolution but you could generate
practically unlimited amount of images (I
normally produce about 100 images in 40 min) and
vacuum in the microscope is great (because you
don't have to vent the camera). Images available
immediately (you don't need to wait for scanning
- few min per plate I believe). So, I don't
think, image plates much suited for biological
applications keeping in mind the price etc. A few
words about resolution. Somebody on ListServer
calculate that single EM film contains about 7
Gbyte of information - it's enormous amount of
information, which we never use 100%. We
normally cropped our images and print them small
(look on the Science magazine pages!), so we
dramatically reduce the resolution on the final
"product". Then, such "final product" becomes
comparable with what we have with digital
cameras. Keep in mind that even super-duper
printers produce gray tones with resolution about
150 dpi (may be 300 at the best). Personally, I
was surprised to see that 3x3" images from my
BioScan 1Mpix camera printed by good inkjet
printer with "gray inks" practically
undistinguishable for the real photo-print. The
disadvantage of such low-res images - you could
not crop or enlarge them, so you just took many
pictures at different magnification. For big
field you could combine a few pictures quite
easily. I hope it'll help. Have a great day, Sergey

P.S. A new argument against image plates just
cames to me: laser. They used, I guess, similar
laser as for phosphorimager. That laser has
limited life - something like 3 years and should
be replaced then. Laser itself is about $20K, so
you need to keep expensive service contract or be
ready to invest in a new laser 2 or 3 year
later... I think, CCD chip and phosphor screen
on digital cameras deteriorated as well- mine is
still OK after 3 years of extensive use.

At 06:52 AM 6/20/2005, you wrote:



------------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I have been given the unenviable task of having to decide between a digital
camera system and a digital imaging plate system (the Ditabis system) to
enable our older TEMs to become fully digital imaging systems. We have Jeol
1200EX and 100CX instruments and a Phillips CM10. Has anyone had experience
of the Ditabis system? In the UK there is only one currently in use, and I
have contact with that person. Our samples are biological samples, some
routine clinical specimens, but also research samples and cell culture
samples. We currently use Kodak em film 4489 and require the replacement
system to have comparable resolution.
Any comments would be gratefully received.

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095

Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu






From MicroscopyL-request-at-ns.microscopy.com Fri Jun 24 10:31:46 2005



From: Microscopy Today :      microscopytoday-at-tampabay.rr.com
Date: Fri, 24 Jun 2005 11:31:00 -0400
Subject: [Microscopy] Microscopy Today July 2005 Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the July 2005 Microscopy Today table of contents. I will close
the subscription list for this issue on Thursday July 7, 2005.

Microscopists in North America and MSA members anywhere may have free
subscriptions. Anyone else may subscribe for US$35 per year (to
PARTIALLY cover postage). All subscriptions at
http://www.microscopy-today.com Thank you.

Ron Anderson, Editor
===============================
A Closer Look at the Nuclear Pore Complex
Stephen W. Carmichael, Mayo Clinic

An Innovative Method for Imaging and Chemical Analysis of Wet Samples in
Scanning Electron Microscopes
Irit Ruach-Nir, QuantomiX Ltd, Rehovot, Israel

An Aberration Corrected (S)TEM Microscope for Nanoresearch
S. Kujawa, B. Freitag, D. Hubert, FEI Company, Eindhoven, The Netherlands

Chemical Imaging with Infrared Microscopy
William J. McCarthy, Thermo Electron Corporation, Madison, WI

A Novel High-Speed Optical Scanning Microscope for Routine Clinical
Applications
D. Frekers1,2, I. Aksit1,2, V. Pilipenko1, K. Bünger;2 1Westfälische
Wilhelms-Universität Münster, 2MedXP-GmbH, Gelsenkirchen, Germany

Easy Guide to Calibrating TEM’s and STEM’s
John McCaffrey, Norrox Scientific Ltd. Ottawa, Canada

Embedding Small Specimens for TEM Examination
Paul Webster, House Ear Institute

The Total Release Method for FIB In-Situ TEM Sample Preparation
T.M. Moore, Omniprobe, Inc., Dallas, TX

EBSD Sample Preparation: Techniques, Tips, and Tricks
Matthew M. Nowell1, Ronald A. Witt2, and Brian W. True1
1EDAX-TSL, Draper, UT, 2EBSD Analytical, Lehi, UT

Funding Opportunities for Acquiring Equipment from Federal Granting
Agencies – Part III: IMR
Session Chair: Debby Sherman, Purdue University

M&M 2004 Core Facility Management – Part IV: Q & A
Moderator: Debbie Sherman

Industry News

NetNotes






From MicroscopyL-request-at-ns.microscopy.com Fri Jun 24 12:57:50 2005



From: claire haueter :      lukeclaire-at-yahoo.com
Date: Fri, 24 Jun 2005 10:57:04 -0700 (PDT)
Subject: [Microscopy] Repair of MT5000 Sorvall Ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Is there anyone out there in the Texas Medical Center
area or near vicinity that you know who can help
repair a MT5000 Sorvall ultramicrotome? It was
received as a gift but is obviously not working right
now. The person in charge of this thinks it might be
just a belt.

Anyone you can recommend?

T.I.A.,

Claire

Claire Haueter
EM Tech, Sr.
Integrated Microscopy Core
Baylor College of Medicine


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 24 13:15:41 2005



From: Steve Chapman :      protrain-at-emcourses.com
Date: Fri, 24 Jun 2005 19:18:44 +0100
Subject: [Microscopy] Judy Murphy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Could Judy Murphy please contact me?

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com


From MicroscopyL-request-at-ns.microscopy.com Fri Jun 24 17:36:28 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Fri, 24 Jun 2005 15:35:37 -0700
Subject: [Microscopy] Re: Re: Re: TEM digitisation: digital plates vs digital camera systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jun 23, 2005, at 6:15 PM, Sergey Ryazantsev wrote:

} This is very common mistake thinking that more pixels gives you 'more'
} resolution. If your sample has, let say 10 nm details and nothing
} smaller, I would expect that quite decent 'resolution' on this
} particular sample would be if you utilize something like 3 pixels per
} 10 nm =} 3.3 nm/pixel. You simply don't need more pixels if there is
} nothing smaller on your sample. Adding more pixels you will basically
} add more noise to the image and that's it. Ultrathin sections of
} biological material, which I called 'biological samples' have quite
} poor resolution because of fixation, dehydratation plastic embedding
} and so on. Therefore, you will just spoil those 110M of pixels...
} It's like some people expected 3A resolution (resolution of the scope
} itself) on 80 nm thick poorly infiltrated chattered plastic sections
} of biological material, no way: 100A may be...
}
} Again, even on my 3.3GHz Pentium 5 with 2 Gb 3400 RAM computer, I do
} see deterioration in performance if I am working on couple 50Mb
} pictures, so you really need super computer to handle your 110 Mb
} pictures. And I am not talking about printing those images: on my
} networked color Xerox Phaser 7700GX with a lot of memory (maximum
} permitted) it takes about 20-30 sec to sent 10-20 Mb image (and more
} than minute to print at 1200 dpi). Your files will simply kill all our
} UCLA network! I think, you guys have better network, probably
} dedicated to handle such giant files.... So, I am just reasoning, why
} from my prospective, there is not much use of image plates in
} biological applications. I am glad that Ditabis is using solid state
} lasers - it definitely will add some juice to the system. By the way,
} how long it takes to scan one plate at maximum resolution?
}
} I do belive that image plate technology has a great potential in
} material science applications when huge dynamic range is critical.
} Have a great day, Sergey
}
Dear Sergey,
I agree that just having more pixels does not show smaller details
than are present in the specimen; however, if the mag is adjusted so
that the smallest details present are, as you suggest, about 3 pixels
across, then the more pixels you have, the larger the field of
view--which may be important. Another way of thinking about this is
that for larger pixels, as occurs with either image plates or CCDs, you
need a higher mag to see the same details, so you will have a smaller
field of view.
For a conventional fixed and stained biological plastic section, there
is no point whatever in having a magnification so large that you can
see details within the clumps of stain (typically ~2 nm), and there may
be no reason to have a mag high enough to distinguish individual stain
clumps (so even the smallest details in such a specimen may be of no
interest), but you still may want to see small gold clusters, e.g., on
antibodies or other labels, over a large piece of tissue, in which case
one would need high enough mag to identify the gold (or even to
distinguish among several sizes of gold) and a large enough field to
cover the entire region of interest in the specimen. It is in this
type of study that film is still ahead of IPs and CCDs due to the
higher resolution--especially with 4489.
Again, when you are talking about the file size that your computer can
handle (today; who knows about tomorrow), this may not be relevant to
the kinds of qualitative work for which film has an advantage. If one
wants to digitize the image, the advantages of film, even for the kind
of qualitative work I mentioned above, become much less pronounced,
since the scanner has larger pixels than the film, and it introduces
noise. In order to produce a 60,000 by 90,000 pixel image from a 6.5 x
9 cm film, the pixel size of the scanner would have to be 1um, and I
have never seen a 25,000 dpi scanner. In fact, it would not surprise
me at all if a CCD or IP with a 15 um pixel size gave a better quality
image than film scanned with a 10 um pixel size or even a 6 um (=4000
dpi, which is common) pixel size.
Finally, there is at least one biological application for which the
large dynamic range of IPs is useful: quantitative electron
diffraction.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Fri Jun 24 19:32:09 2005



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 24 Jun 2005 17:32:07 -0700
Subject: [Microscopy] Re: Re: Re: Re: TEM digitisation: digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Bill
I completely agree with you: there are a number of application when large
view field is important and then old friend film or image plate may be a
solution. My point was that because of very low actual resolution on
ultrathin sections in particular, one don't need too much pixels to show
sample's details properly. For instance, my BioScan 600W camera being 1
Mpix has a view field size bigger than film - it was a whole point for
purposing that particular camera. So, I actually, have bigger than on the
film field size on my digital images. The price for that - low pixel
conts. But - I don't need much pixels to represent trustfully what I have
on my sections. It includes immuno-staining. I need to clarify: top mount
cameras have lover magnification than on the film, therefore they have
visible larger view area. In my particular case, my microscope do not
permits to take pictures at lower than x3K magnification, but I am able to
take pictures at x1.5K on digital camera, so I have more "field". Keeping
in mind your arguments in favor of really large view field I still keep my
dark-room running and 50 sheets of film is in the microscope. They are
sitting in the scope 3rd year: nobody wants to use film since digital
camera arrived. This is just practical observation: my users don't need
large field and high pixels count! What they need - to generate images
quickly and as many as they need, to be able to print them immediately,
transfer them through existing network (not 110 Mb) - digital camera suites
their need perfectly. For some rare work, I still have film
available. This is my practical solution. I think, purposing digital
camera was my best investment by the way.

As for scanning EM films - you absolutely right - film is
superior! Personally, I prefer old friend - film. Have a great
weekend, Sergey

At 03:35 PM 6/24/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
and UCLA Institute of Nanotechnology
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095


Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu





From MicroscopyL-request-at-ns.microscopy.com Sat Jun 25 09:57:57 2005



From: LDM Microscopy :      ldmm-at-risc4.numis.northwestern.edu.edu
Date: Sat, 25 Jun 2005 09:57:16 -0500 (CDT)
Subject: [Microscopy] Re: Postdoctoral Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Postdoctoral Position Available

A postdoctoral position is available at Northwestern to work
on a couple of projects, one involving low-loss energy loss
spectroscopy of surface plasmons (aloof spectroscopy) in
collaboration with a number of other faculty, the other a range
of electron microscopy on oxide materials related to fuel cells.
There will also be opportunities to interact with students/postdocs
working on several other projects including oxide surfaces, atomic-scale
tribology, heterogeneous catalysis and precession electron diffraction.

A strong general background in transmission electron microscopy and energy
loss spectroscopy is required, and some experience in the specific
research areas would be useful.

Applicants should apply by email only to L - marks -at- northwestern.edu
including the names and emails of three referees and a CV not more
than 3 pages in length including refereed publications and presentations.
Large attachments will invoke the delete button.

-----------------------------------------------
Laurence Marks
Department of Materials Science and Engineering
MSE Rm 2036 Cook Hall
2220 N Campus Drive
Northwestern University
Evanston, IL 60201, USA
Tel: (847) 491-3996 Fax: (847) 491-7820
email: L - marks -at- northwestern . edu
http://www.numis.northwestern.edu
-----------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Sun Jun 26 09:24:20 2005



From: david.griffiths-at-veths.no (by way of MicroscopyListserver)
Date: Sun, 26 Jun 2005 09:23:37 -0500
Subject: [Microscopy] viaWWW: Need LM sections from hpma blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (david.griffiths-at-veths.no) from http://www.microscopy.com/MLFormMail.html on Sunday, June 26, 2005 at 03:40:39
---------------------------------------------------------------------------

Email: david.griffiths-at-veths.no
Name: David Griffiths

Organization: Norwegian School of Veterinary Science

Title-Subject: [Microscopy] [Filtered] Need LM sections from hpma blocks

Question: We have a number of tissue blocks embedded in Technovit 8100 and other hpma's, but we aren't set up for sectioning yet. Is there anyone with with spare capacity who can cut sections of these blocks for us, for payment of course? If so could you please indicate your interest and we can sort out the details. The typical block would be about 10mm x 10mm, and we would like sections at 1.5 - 2.0 microns, mounted on a glass slide. About 10 - 15 sections per block at the moment.
Thanks,
Dave Griffiths
Section of Anatomy
Norwegian School of Veterinary Science

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Sun Jun 26 12:45:08 2005



From: Carol Heckman :      heckman-at-bgnet.bgsu.edu
Date: Sun, 26 Jun 2005 13:43:52 -0700
Subject: [Microscopy] Re: Re: Re: TEM digitisation: digital plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sergey-
Actually, there are two additional cases where the 3.3 nm/pixel
wouldn't help you with a problem. One is where you are trying for
automated extraction of the images of 5-nm gold particles, in order
to overlay them on the image. We have a procedure for this, and you
do have to have 3 pixels across the 5-nm image (minimum). It is
unpublished as yet (I am trying to get my former student to add it to
our on-line protocols). Another case where one would need } 3 pixels
across a feature is if the curvature of a contour is to be measured.
Here, pixellation artifact prevents any meaningful measurements from
being taken. To be specific, with a square array, one can only
measure angles at 90 degrees apart (straight to right and left, up
and down) and at 45 degrees apart (the corners). It is not possible
to derive a meaningful measure under these circumstances, and the
solution is to take more pixels and drop two between those subjected
to the final measurement. We published this solution in 1980 (if I
recollect correctly).

In any case, there are problems where lots of pixels in the array are
essential. The problem happen to be in the arena where the image
processing can be automated to effect, and that is the main advantage
of taking a digital image i.m.h.o. So the deck is not stacked
against the Ditabis technology -- the field just hasn't caught up
with the potential applications of the digital technology.
Carol
P.S. No commercial interest in Ditabis or its competitors.

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu
http://www.bgsu.edu/departments/biology/facilities/MnM
___________________________________________________________________________


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 08:13:15 2005



From: morten.laanre-at-imbv.uio.no (by way of MicroscopyListserver)
Date: Mon, 27 Jun 2005 08:12:33 -0500
Subject: [Microscopy] viaWWW: Zeiss 40x "antiflex" objective

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (morten.laanre-at-imbv.uio.no) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, June 27, 2005 at 05:20:55
---------------------------------------------------------------------------

Email: morten.laanre-at-imbv.uio.no
Name: C.M.M. Laane

Organization: Institute of Molecular Bioscience,UiO Norway

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: Does anyone have a Zeiss 40x "antiflex" objective for 160mm tube length for sale.Old catalog no. 46 17 54 (or 46 17 53) ?
The lens is needed for studies of cell attachment to substrate (surface).

Sincerely: C. Morten Motzfeldt Laane
Institute of Molecular Bioscience (IMBV)
University of Oslo, Norway
P.o.Box 1041, N-0316 Blindern, Norway

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 13:49:34 2005



From: Sergey :      sryazant-at-ucla.edu
Date: Mon, 27 Jun 2005 11:48:28 -0700
Subject: [Microscopy] Re: Re: Re: Re: TEM digitisation: digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Carol
I absolutely agree: there are lot of cases when you need more pixels. From
another hand, if you need more pixels for automatization - image plate
technology is not the best: you need manually load/unload plates, load them
into scanner, remove from scanner, load into cassette, load in microscope
etc... and after that you'll have 50 images without proper identification
(not everyone has last model microscopes). Keep in mind that scanning
itself at max resolution will take a while. I was asking Bill Miller a few
times how long it would take to scan a single plate. No answer. I would
imagine, it's comparable with scanning time on phosphorimager- about 20 min
3x4" area. If so (correct me Bill if I wrong) - 16 hours for 50
plates... Good digital camera is much, much better for automatization. I
am not even speaking about such wonderful features as "auto-tune", when
microscope automatically aligned at degree human eye could not. You also
could combine digital camera with energy filters etc - this is a way for
automatization. I used to work on new Hitachi microscope (don't remember
the model, 7200?) - they integrated 3D reconstruction from tilted series
into hardware - you just press knob and microscope (with digital camera)
made tilt series and reconstruct 3D image from it basically in real time -
you may not do anything like that with image plates, I am sorry Bill. Have
a great day. Sergey

At 01:43 PM 6/26/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 16:05:22 2005



From: Al Harmon :      aharmon-at-mvainc.com
Date: Mon, 27 Jun 2005 17:09:26 -0400
Subject: [Microscopy] ultra-microtomy of water soluable crystals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have some very tiny spheres (3-5 microns in diameter) that I need to
obtain a thin section for TEM analysis, they are water soluable and
crumble easily. What I have done already is to mount them on blank stubs
with Extec and microtomy with limited results. Since they can't be
floated onto water I have been catching them dry onto carbon coated
copper TEM grids. They move quite a bit under the beam and the
crystalline interior crumbles.
Any thoughts would be appreciated.

Al Harmon
MVA Scientific Consultants, Inc.
"aharmon-at-mvainc.com"



From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 16:12:37 2005



From: Bill Miller :      microbill-at-mohawk.net
Date: Mon, 27 Jun 2005 17:11:56 -0400
Subject: [Microscopy] Plate scanning time

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sergey - you must have missed the scanning time e-mail, I sent it to
you personally as you requested - we've had so many with the same
subject it may just gotten lost in the clutter... 2.5 minutes at 15
um and ~10minutes at 7.5um or about 1 hour to load, read and unload
the full load of 20 plates - less plates or lower resolution means
less time, of course..

A digital camera is certainly faster but if you need the resolution,
as she has indicated, they are quite costly - 4k x 4k, 14um pixel
cameras (only half the pixels of the 15um plate scanner) cost upwards
of $200k. I sell cameras for tomography as well and NOBODY does
tomography fast enough to have it qualify as "real time" . Even
the TVIPS 1k camera with } 12 frames per second takes quite some
time to do a full tilt series ( usually over 100 images). That said,
I'd never sell a plate system to anyone who was doing tilt series
topography - it's totally the wrong tool. Any application that
requires hundreds of images is best done with a CCD camera rather
than plats or film.


} -------------------------------------------------------------------------------
}
} Carol
} I absolutely agree: there are lot of cases when you need more
} pixels. From another hand, if you need more pixels for
} automatization - image plate technology is not the best: you need
} manually load/unload plates, load them into scanner, remove from
} scanner, load into cassette, load in microscope etc... and after
} that you'll have 50 images without proper identification (not
} everyone has last model microscopes). Keep in mind that scanning
} itself at max resolution will take a while. I was asking Bill
} Miller a few times how long it would take to scan a single
} plate. No answer. I would imagine, it's comparable with scanning
} time on phosphorimager- about 20 min 3x4" area. If so (correct me
} Bill if I wrong) - 16 hours for 50 plates... Good digital camera is
} much, much better for automatization. I am not even speaking about
} such wonderful features as "auto-tune", when microscope
} automatically aligned at degree human eye could not. You also could
} combine digital camera with energy filters etc - this is a way for
} automatization. I used to work on new Hitachi microscope (don't
} remember the model, 7200?) - they integrated 3D reconstruction from
} tilted series into hardware - you just press knob and microscope
} (with digital camera) made tilt series and reconstruct 3D image from
} it basically in real time - you may not do anything like that with
} image plates, I am sorry Bill. Have a great day. Sergey




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 16:35:31 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 27 Jun 2005 14:34:48 -0700
Subject: [Microscopy] Re: ultra-microtomy of water soluable crystals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jun 27, 2005, at 2:09 PM, Al Harmon wrote:

} They move quite a bit under the beam and the crystalline interior
} crumbles.
}
Dear Al,
You could try carbon coating after the particles have been picked up
on the carbon-coated grids, and you could try a lower beam current.
The movement under the beam indicates either charging, heating, both,
or some other process--beam-induced specimen movement has not been
completely characterized--and increasing electrical and thermal
conductivity, or lowering the rate at which charge and/or heat
accumulates on the specimen will, to some extent, ameliorate the
problem.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 16:37:50 2005



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Mon, 27 Jun 2005 16:37:06 -0500
Subject: [Microscopy] TEM Negative digitization times.

Contents Retrieved from Microscopy Listserver Archives
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Sergey

Stating that it takes 20 minutes to scan a negative at high
resolution without
specifics of what you mean or without comparisons to actual modern data
does not do justice to the purpose of this forum or the technological
advancements
that are in the scientific market today.

I have just gone to my older high resolution scanner (Leafscan 45)
here at ANL and
have digitized a 3 1/4 x 4 " negative at 1270 dpi ~ or a
resolution of 20 microns/pixel.
The image size was 5080 x 4128 pixels (21 Megapixels) and 16 bits deep.
The total time from the point that I mounted the negative in the holder
to when I had a digitized image on my screen was just under 3 minutes,
not 20 minutes. This is a fairly old scanner and I know that
newer ones are faster
and have higher resolutions (abeit not always with as good a bit depth).

One certainly could have gotten a similar image with a CCD camera by taking
multiple images (the number varying with the resolution of the camera)
but not in the same amount of time or cost. It would require
either a very large and expensive CCD camera or off-line stitching of
multiple images to merge and align the individual images together to
form an equivalent single image.

I use both technologies in my research, choosing the best technology
for the image
as appropriate, just as I would selecte the most appropriate
microscope to analyze
a specimen.

Let me remind everyone, that we all have the responsibility to make sure that
we are reasonably factual, accurate and up-to-date when we
point out the pros and cons of any technology. Using significantly out of
date or inaccurate information does not help disseminate useful knowledge
to the community, which is one of the purposes of this forum.


Nestor
Your Friendly Neighborhood SysOp

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
NEESLab: http://neestpm.mcs.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================


From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 16:43:40 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Mon, 27 Jun 2005 14:42:57 -0700
Subject: [Microscopy] F30H objective aperture & low dose--solution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Lists,
Thanks everyone for the many helpful comments and suggestions. After
a series of investigations, we tried something that, in retrospect, was
obvious. When a smaller objective aperture was inserted, the problem
became less severe, leading to the conclusion that somehow the larger
objective aperture was out of position. Some of the possibilities are
that the aperture was not placed properly in the holder, that the foil
came loose from the body of the aperture, or that the tip was being
displaced when moved to the farthest-in position. In our case the we
still do not know exactly what occurred, but the apertures were
properly positioned and looked fine. This can occur in a microscope
where the aperture is in the back focal plane, as well as one like the
F30 where the aperture is not, so if you experience a similar problem
with your aperture not being centered in Focus state when it is
centered in Exposure state, see if this occurs just with one aperture
or is present for all the apertures.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 18:39:18 2005



From: Sergey :      sryazant-at-ucla.edu
Date: Mon, 27 Jun 2005 16:38:10 -0700
Subject: [Microscopy] Re: TEM Negative digitization times.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Nestor
In my message I was talking about image plates, not negatives. In my
speculation about time necessary to scan image plate I based on estimation
from scientific instrument, which is using very similar technology as a
Ditabis plate reader: phosphorimager. It uses basically the same image
plates and "imaging technology" is very similar if not identical. Latest
model of our Departmental phosphorimager Typhoon 9410 needs about 40 min to
scan 2x3" area with resolution of 10 um (see specification on instrument).
For 25 um, which perhaps comparable with Ditabis it would be 15-20
min. You may not compare optical scanner with image plate technology -
it's absolutely different instruments and principles! By the way, if you
will use good specialized optical scanner for difractogtramm (which gives
you optical density) - it will take much longer than 2-3 min to scan EM
size film, so I am quite sure that my approximation is quite right and this
is exactly why I was talking about that: many people would think similar to
you that "scanning is not a big deal". To get correct readings in OU is
basically a big deal even nowadays. "Household" scanners may not be used
for quantitative analysis - they are not calibrated and sometime not
linear. Their "color" sensor (depends from construction) may compromise
the data. In my message, I clearly stated that I was trying to obtain
technical data from Ditabis and they refuse to answer, so I have to
speculate instead using real data from manufacturer. Basically, even if it
takes according you just 3 min per plate - still, it will take 2.5 hours to
scan the standard set of 50 plates. Not very impressive to me. I am sorry
if I broke some rules AGAIN, I am naturally rule-breaker, I think most of
the science progress based on breaking somebody's rules (your boss
perhaps?). Have a good day, Sergey

At 02:37 PM 6/27/2005, you wrote:


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From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 20:45:18 2005



From: Bill Miller :      microbill-at-mohawk.net
Date: Mon, 27 Jun 2005 21:44:37 -0400
Subject: [Microscopy] Re: Re: TEM Negative digitization times.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sergey - just for comparison sake lets try to compare apples to
apples... figure out how long it would take to do 50 film images with
darkroom work - developing, drying , printing, drying - it's been so
long since I've had to actually do that I'd no longer feel confident
in even venturing a guess - but not minutes for sure

and then with your 1k camera shot to cover the same area as the film
at the same resolution as the plates with image tiling - that's
roughly 35-40 CCD images per 6k x 5k image plate (don't forget the
necessary overlap for the tiling) for a total of ~1800 CCD images --
you claimed that you took 100 images in 40 minutes so the math works
out to roughly 720 minutes (12 hours) BEFORE tiling

Two and a half hours to viewable images with the plates doesn't look
so bad from a time stand point when you look at the process
objectively - particularly since for most of the time the scanner is
doing all the work leaving you free to do something else. If you
don't need or want the resolution then it's rather hard to make a
rational comparison between low pixel count CCDs and imaging plates
since the image plates would not be the proper imaging device for
such an application anyway. No body is trying to talk anybody into
using the plates for anything other than what they are good for. If
I had to pull a 60 foot trailer I wouldn't pick a motorcycle to do it
with just because it was faster than a truck.

That said, the plates may still not be the best answer for many
application. When short turn around time is critical CCD cameras are
the answer. There is an endless list of applications where one
technology is clearly more suitable than another. Pick the one that
work for you.

Regards - Bill

At 07:38 PM 6/27/2005, you wrote:


} ------------------------------------------------------------------------------
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From MicroscopyL-request-at-ns.microscopy.com Mon Jun 27 23:53:42 2005



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Mon, 27 Jun 2005 23:52:58 -0500
Subject: [Microscopy] TEM Negative & Image Plate digitization times

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bill

It is good to see factual information about scanning times for image plates. I also concur with your comments on using this technology for tomography.

The digitization times you presented are certainly consistent with my experiences using a high resolution high end negative scanners like the Leafscan 45, which is certainly not a run of the mill household scanner as the un-informed might presume.

As a followup, I would like to hear of comparisons using the older technology namely-negative drum scanners if anyone is still using this technology. I haven't used one of these used in years, and only vaguely remember using one over a decade ago here at ANL, it unfortunately has long since disappeared so I cannot test it out nor do I still have access to the original instrument specifications for comparison.

If anyone has additional real experience with plate or other high end negative scanners to add to this discussion it would be appropriate to post that data for the archives and to complete this discussion.

Finally, out of interest I checked on the specification of the Typhoon 9410 which was used as a basis for some scanning time estimates posted earlier in this discussion thread, which seemed unrealistic to me.

I was surprized to find out that the Typhoon 9410 is neither a negative nor image place scanner, but rather is a "high performance gel and blot imager ". Checking the technical specifications I see that while it has the same bit depth resolution as the Leafscan 45, it only has a uniformity of +/5% over the scan area, making this unsuitable for TEM work. The Typhoon manufacturer GE, in addition, makes absolutely no claims or has any application notes on its WWW site which indicate it is suitable for use with TEM negatives or image plates, nor is it apparently designed to be used for TEM application. It's scanning times are optimized for the detection of signal in gel/blot image data and not for data relevant to the discussion in this thread. In light of this fact, the performance characteristics estimated from an instrument like the Typhoon9410 and then extrapolated to TEM negative and/or image plate scanning are, in my opinion, a comparison which carries no technical merit.


Nestor
Your Friendly Neighborhood SysOp


Disclaimer: I have no financial interests in any of the imaging/scanning technologies discussed herein.



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 09:31:27 2005



From: Tom W Bargar :      tbargar-at-unmc.edu
Date: Tue, 28 Jun 2005 09:30:42 -0500
Subject: [Microscopy] EDS Detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





A couple of questions. First, I would like to receive information from
vendors on their current models of elemental x-ray detectors. I would also
like to hear a comparison between the detectors that use liquid nitrogen
and those models that don't need liquid nitrogen cooling. Secondly, while
we hope to get a new SEM, we may not be able to, in that event I would like
to know if a EDX unit could still be fitted to our JEOL T220A SEM. As
always any information is appreciated from everyone.

Tom Bargar
Core Electron Microscopy Research Facility
University of Nebraska Medical Center
986395 Nebraska Medical Center
Omaha, NE 68198-6395
phone: 402-559-7347
tbargar-at-unmc.edu



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 11:25:01 2005



From: Andrew Johnstone :      johnstone-at-uky.edu
Date: Tue, 28 Jun 2005 12:17:12 -0400
Subject: [Microscopy] TEM - florescent bead labeling tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Checking if anyone knows of any new florescent bead labeling techniques when marking specific sites on tissues. Specifically, something that will not dissolve in PO. A polystyrene replacement?

Thanks

~~~~~~~~~~~~~~~~~~~~~~~
Andrew F.M. Johnstone
Deptartment of Biology
Cooper Lab
University of Kentucky
email: johnstone-at-uky.edu



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 13:25:49 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Tue, 28 Jun 2005 11:24:03 -0700
Subject: [Microscopy] Re: TEM Negative & Image Plate digitization times

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jun 27, 2005, at 9:52 PM, Nestor J. Zaluzec wrote:

} If anyone has additional real experience with plate or other high end
} negative scanners to add to this discussion it would be appropriate
} to post that data for the archives and to complete this discussion.
}
Dear Nestor,
I have had experience with scanners ranging from a CCD used to image
the light transmitted through a negative, through a linear diode array,
and a HiScan drum scanner to a Perkin Elmer microdensitometer. For
images, where generally the intensities do not vary rapidly over small
distances, and where the OD is centered around 1 and ranges from a few
tenths to, say, 2, any of the scanners will give a decent
representation of the image, and the fidelity of quantitation depends
on the number of pixels, the response time of the electronics, and the
stability of the illumination (among other variables). For electron
diffraction patterns, where the range of ODs is from ~0.001 to ~4, and
can vary greatly over distances on the order of tens of um, accurate
quantitation cannot be obtained unless the illuminated area of the
negative is the same as the scanning pixel. This is due to the fact
that only a small fraction of the light transmitted through adjacent
areas and scattered in the detector housing will give a falsely low OD
reading. For quantitation of ED patterns, only a microdensitometer,
such as the Perkin Elmer, will give sufficiently accurate data, and
then only at a fairly slow scan speed and small pixel size. I am not
up to date on the latest improvements in scanning times, but back in
the day such a scan would take hours for each negative. I have no
relationship to Perkin Elmer except as a former satisfied user.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 13:52:43 2005



From: Sergey :      sryazant-at-ucla.edu
Date: Tue, 28 Jun 2005 11:51:50 -0700
Subject: [Microscopy] Re: TEM Negative & Image Plate digitization times

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Nestor
You need to read my postings: I did not claim that Typhoon is a "scanner"
for negatives or have something to do with EM. Nevertheless, it's the best
on the market reader for "IMAGE PLATES". Image plate technology used in
many areas when relatively high energy particles (alpha/beta particles etc)
or gamma need to be detected. It's commonly used in molecular biology when
people used radioactive (32P, 14C, 3H) labelling, DNA for instance. You
separate your stuff on the gel and detect radioactivity using image plate.
In this area image plate technology is well established and
respected. Because (and I mentioned it in my original posting) image plate
technology is the same for EM and for other application it's legit and
even interesting to compare performance of different instruments- "image
plate readers" (correct name for it). From another hand I could not
understand why you are trying to compare optical scanners with image plate
readers? I think it's simply not legit to make any bridge between (even
non-household) optical scanner and image plate reader because
of differences in principles of operation - you have to know better, you
read manual to Typhoon. Leafscan 45 based on my quick research on Internet
- it's very good professional scanner for general photography, it scans
color and B&W slides and transparencies etc - it's not specialized scanner
for EM, it's not a densitometer ether- similarly as Typhoon is not
specialized reader for EM. If you think you may compare the data from
non-EM general scanner and image plate readers, than, my comparison of
Typhoon and Diabis is even more legit! Typhoon will scan Diabis image
plates (as any other) perfectly, by the way. 5% uniformity - this is what
you may expect from image plate readers due image plate properties, I
guess, and this is another point to think twice before using this
technology. I have about 10 years experience with image plates and readers
(in molecular biology where this technology is proven to have numerous of
advantages), so I am quite confident that information I provided is correct
and it's not my fault if somebody do not read the original postings and
misinterpret it: may be it's just because my broken English? I am sorry
for that. Sergey

At 09:52 PM 6/27/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 15:16:00 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Tue, 28 Jun 2005 15:15:01 -0500
Subject: [Microscopy] Re: ultra-microtomy of water soluable crystals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Al,

We've been working with NT-MDT and Leica on a new AFM/Ultramicrotome hybrid that could provide an answer to your dilemma. The fully automated system will be announced officially later this year but the manual version was shown at Cell Bio last December and will be at the SPM '05 in Sapporo this month. It allows you to cut then image in one system, without having to catch sections. The results can be fed into a 3D reconstruction program (NT-MDT has tried, successfully, with Media Cybernetic's 3D Constructor) to generate 3D images and resulting image analysis. The AFM uses local differences in elasticity and hardness (among other modalities) to create contrast. If you would like to send some samples, we can forward them to NT-MDT and have them run them for you. You can contact me off-line for details.

Hope this is helpful.

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call us at (972)954-8011.

At 04:09 PM 6/27/2005, Al Harmon wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 16:00:05 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Tue, 28 Jun 2005 13:59:16 -0700
Subject: [Microscopy] TEM Negative & Image Plate digitization times

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Another issue to consider in negative scanning vs. image plates, is that
only a fraction of the negatives would ever get scanned because
examination of the negatives with a magnifier (human scan?) typically
reveals that too many of the negatives are not perfect, due to focus,
drift, or some other glitch. I can't quote a number, but for 30 years it
always seemed too high, either due to my skill, or in later years to my
being too picky (or both). With image plates, every exposure needs to
get scanned to be observed.

John Mardinly
Intel

This opinion is the opinion of the author, not of Intel corporation.
-----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-aaem.amc.anl.gov]
Sent: Monday, June 27, 2005 9:53 PM
To: microscopy-at-microscopy.com

Bill

It is good to see factual information about scanning times for image
plates. I also concur with your comments on using this technology for
tomography.

The digitization times you presented are certainly consistent with my
experiences using a high resolution high end negative scanners like the
Leafscan 45, which is certainly not a run of the mill household scanner
as the un-informed might presume.

As a followup, I would like to hear of comparisons using the older
technology namely-negative drum scanners if anyone is still using this
technology. I haven't used one of these used in years, and only
vaguely remember using one over a decade ago here at ANL, it
unfortunately has long since disappeared so I cannot test it out nor do
I still have access to the original instrument specifications for
comparison.

If anyone has additional real experience with plate or other high end
negative scanners to add to this discussion it would be appropriate to
post that data for the archives and to complete this discussion.

Finally, out of interest I checked on the specification of the Typhoon
9410 which was used as a basis for some scanning time estimates posted
earlier in this discussion thread, which seemed unrealistic to me.

I was surprized to find out that the Typhoon 9410 is neither a negative
nor image place scanner, but rather is a "high performance gel and
blot imager ". Checking the technical specifications I see that while
it has the same bit depth resolution as the Leafscan 45, it only has a
uniformity of +/5% over the scan area, making this unsuitable for TEM
work. The Typhoon manufacturer GE, in addition, makes absolutely no
claims or has any application notes on its WWW site which indicate it
is suitable for use with TEM negatives or image plates, nor is it
apparently designed to be used for TEM application. It's scanning
times are optimized for the detection of signal in gel/blot image data
and not for data relevant to the discussion in this thread. In
light of this fact, the performance characteristics estimated from an
instrument like the Typhoon9410 and then extrapolated to TEM negative
and/or image plate scanning are, in my opinion, a comparison whic!
h carries no technical merit.


Nestor
Your Friendly Neighborhood SysOp


Disclaimer: I have no financial interests in any of the imaging/scanning
technologies discussed herein.





From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 16:24:23 2005



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 28 Jun 2005 16:23:43 -0500
Subject: [Microscopy] TEM: digital and/or film scope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Theoretical Question: if you were purchasing a TEM equipped with a
high quality (2k x 2k) digital camera would you still opt for the
film camera as well? Just wondering.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 16:32:42 2005



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 28 Jun 2005 14:23:19 -0700
Subject: [Microscopy] Congo red & protein fibrils?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am told by someone who visits my lab that protein fibrils treated with
congo red will show an apple green birefringence in crossed polars.

She has brought samples for us to look at, but we cannot see the green
birefringence. She 'knows' there are fibrils present by another type of
assay.

Has anybody heard of this technique? I think I can see the stained fibrils,
but nothing shows with the crossed polars. Maybe I don't know what I should
be looking for, maybe it is very subtle. Maybe it doesn't really work.

If you have heard of this technique and maybe know a trick or two, let me
know so I can help this person.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 16:41:51 2005



From: Philip Oshel :      peoshel-at-wisc.edu
Date: Tue, 28 Jun 2005 16:41:07 -0500
Subject: [Microscopy] Microscopy texts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Microphiles,

I'm looking for a couple of EM texts, and having no luck with the
on-line used book sources:
M. A. Hayat, "Fixation for Electron Microscopy"
Reimer and Hawkes "Scanning Electron Microscopy : Physics of Image
Formation and Microanalysis"
Anyone happen to have an extra copy in good or better condition that
they might be willing to part with?

Phil
--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 17:11:09 2005



From: Damian Neuberger :      neuberger1234-at-comcast.net
Date: Tue, 28 Jun 2005 17:10:25 -0500
Subject: [Microscopy] Microscopy texts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Phil,
If you want, for the price of mailing them, Hayat Principles and Techniques
of Electron Microscopy Biological Applications Vols 1 and 3, 1970 and 1973
respectively. Kind of old but unless someone wants them, they go into the
trash along with a bunch more.
Damian

-----Original Message-----
} From: Philip Oshel [mailto:peoshel-at-wisc.edu]
Sent: Tuesday, June 28, 2005 4:41 PM
To: Microscopy-at-microscopy.com

Microphiles,

I'm looking for a couple of EM texts, and having no luck with the
on-line used book sources:
M. A. Hayat, "Fixation for Electron Microscopy"
Reimer and Hawkes "Scanning Electron Microscopy : Physics of Image
Formation and Microanalysis"
Anyone happen to have an extra copy in good or better condition that
they might be willing to part with?

Phil
--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm





From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 17:16:24 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Tue, 28 Jun 2005 18:15:35 -0400
Subject: [Microscopy] Re: Congo red & protein fibrils?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,
The trick here is that you have to think in terms of
dichroism rather than birefringence. What happens with congo red (at
least in theory) is that it binds to the substrate in an oriented
way. So if you have a substrate that is oriented then the congo red
molecules themselves become oriented. Now congo red happens to be
good at absorbing linearly polarized light oriented along one axis of
the molecule and bad along the perpendicular axis. So a congo red
stained sample should act like a polarizing filter, itself. So to see
your apple green signal, what you do is to remove the analyzer from
the system, have just the polarizer (you can do it the other way if
you want, it doesn't matter). Then rotate the sample through at
least 90 degrees. You are using the sample to make "crossed
polarizers". The apple green should show up when the sample fibers
are at some defined orientation to the polarizer and the signal
should disappear at +/- 90 degrees to that orientation (no getting
more specific without knowing which way the fibers go and how the
congo binds to them).

You get green presumably becaue the congo red absorbs red and
blue better and so more green leaks through. Actually, polarizing
filters themselves are often made by aligning really strong crystals
that have dichroic abosorption, and they too have a green cast
because it is difficult to have no wavelength dependence to the
dichroism.

Keep in mind that congo red stained fibers are going to be a
whole heck of a lot less good at being a polarizing filter than a
real polarizing filter, so the green signal may be pretty faint. But
its tell tale appearance and disappearence at +/- 90 gives you a
story. You can play with using green light (or other wave bands) but
I think one's eyes are most senstivite to seeing differences in color
rather than intensity.

Hope this helps,
Tobias
}
}
} I am told by someone who visits my lab that protein fibrils treated with
} congo red will show an apple green birefringence in crossed polars.
}
} She has brought samples for us to look at, but we cannot see the green
} birefringence. She 'knows' there are fibrils present by another type of
} assay.
}
} Has anybody heard of this technique? I think I can see the stained fibrils,
} but nothing shows with the crossed polars. Maybe I don't know what I should
} be looking for, maybe it is very subtle. Maybe it doesn't really work.
}
} If you have heard of this technique and maybe know a trick or two, let me
} know so I can help this person.
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243


From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 18:05:14 2005



From: Aleksandr.Mironov :      Aleksandr.Mironov-at-manchester.ac.uk
Date: Wed, 29 Jun 2005 00:04:08 +0100
Subject: [Microscopy] Re: Congo red & protein fibrils?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

To my knowledge green birefringence after Congo Red treatment appears
when your protein has a lot of b-sheets in its structure. Amyloids
formed from different proteins (APP, prion etc.) show such phenomenon.
So, perhaps, the sample you was looking at did not have amyloid
conformation.

Regards,
Aleksandr Mironov
EM Unit
University of Manchester


On 28 Jun 2005, at 22:23, Jon Krupp wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} --------
}
} I am told by someone who visits my lab that protein fibrils treated
} with
} congo red will show an apple green birefringence in crossed polars.
}
} She has brought samples for us to look at, but we cannot see the green
} birefringence. She 'knows' there are fibrils present by another type of
} assay.
}
} Has anybody heard of this technique? I think I can see the stained
} fibrils,
} but nothing shows with the crossed polars. Maybe I don't know what I
} should
} be looking for, maybe it is very subtle. Maybe it doesn't really work.
}
} If you have heard of this technique and maybe know a trick or two, let
} me
} know so I can help this person.
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 18:50:11 2005



From: Sally Stowe :      Sally.Stowe-at-anu.edu.au
Date: Wed, 29 Jun 2005 09:49:10 +1000
Subject: [Microscopy] ] Re: TEM Negative & Image Plate digitization times-contrast range

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An evaluation of a Ditabis scanner a few years ago left the impression that
it was slightly less convenient to use than film, although now that less
film is being processed that may be changing. But the very great advantage
in our applications was the dynamic range rather than spatial resolution. 32
bit is something else.
Diffraction patterns easily showed features that would have had to be
carefully sought out using film. For biological work, IP plates offer the
possibility of much more frequent use of unstained material - looking at the
real tissue rather than a heavy metal incrustation of osmium, lead or
uranium, in embedded as well as frozen material. I would be interested to
know if anyone has been doing much along these lines?

IP is complementary to CCD cameras at the moment, rather than a practical
replacement. The immediacy of the CCD camera is such an advantage that its
hard to remember how we worked without it. My take on the "2K x 2K CCD vs
film camera" question is "2k by 2K...keep the film just in case. But once
4K by 4k or more comes in affordably..... time for some serious re-design of
the TEM!

My two cents worth..

Cheers
Sally



Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
ANU CRICOS#00120C



} -----Original Message-----
} From: Bill Tivol [mailto:tivol-at-caltech.edu]
} Sent: Wednesday, 29 June 2005 4:24 AM
} To: microscopy-at-msa.microscopy.com
} Subject: [Microscopy] Re: TEM Negative & Image Plate digitization times
}
}
}
}
} ---------------------------------------------------------------
} ---------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
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}
}
}




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 19:05:37 2005



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 29 Jun 2005 12:05:57 +1200
Subject: [Microscopy] Re: EDS Detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
} A couple of questions. First, I would like to receive information
} from vendors on their current models of elemental x-ray detectors. I
} would also like to hear a comparison between the detectors that use
} liquid nitrogen and those models that don't need liquid nitrogen
} cooling. Secondly, while we hope to get a new SEM, we may not be
} able to, in that event I would like to know if a EDX unit could still
} be fitted to our JEOL T220A SEM. As always any information is
} appreciated from everyone.
}


Whatever you do, think carefully about the detailed mechanical design of
whatever detector you end up buying, and, if possible, the manufacturer's
reputation for standing behind their products.

I have an approximately 5-year-old PGT Prism 2000, the sort which has an
adjustable dewar angle to allow it to be used at a variety of takeoff angles.

At 2 1/2 years old, its LN2 consumption had steadily increased to the point
where it had to be refilled every second day, so I returned it to PGT to have
the leak fixed and to be re-evacuated, for over a thousand bucks.

When I (eventually) got it back, the LN2 consumption was OK, but over the
next two years it steadily deteriorated again, quite spontaneously (no
warmups or other mishaps), and, to my extreme disappointment, PGT's
non-negotiable attitude is "it was OK when it left here, it must have
developed another leak".

This defies common sense --- we're talking here about a detector which
twice experienced a dewar vacuum deterioration, and whose leak was
apparently not fixed when it was returned to the factory.

I don't think it's a leaky window, as it's been on my SEM under vacuum 24/7
all the time, so my suspicion falls on the O-ring-sealed elbow.

Unfortunately, the PGT vacuum port is non-standard and is on the top
surface right underneath the dewar, and, even more ridiculously, the PGT
adaptor (US$500!!!) won't fit onto it if the dewar is tilted to be vertical for a
40 degree takeoff angle, so, you guessed it, AFTER evacuating the
detector, the bolts holding the elbow joint have to be slackened so that the
elbow can be adjusted to the correct angle! This, of course, placed the
bright shiny new vacuum at risk.

The guys in the excellent workshop to which I have access have now made
me an adaptor which will fit onto the untilted detector, and when I work up
the courage I will repump it myself.

Of course, everyone makes their own purchase decisions, but I will never
again buy a detector which:

- has an adjustable elbow
- has a non-standard vacuum port
- is made by a manufacturer who won't guarantee the vacuum integrity of
the dewar for five years following manufacture or factory repair (barring
accidents)
- is made by PGT.

In the meantime I fill the damned thing every day, including Saturdays and
Sundays, with considerable resentment.

cheers

rtch



--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 19:06:17 2005



From: David Elliott :      Elliott-at-arizona.edu
Date: Tue, 28 Jun 2005 17:05:32 -0700
Subject: [Microscopy] Re: TEM: digital and/or film scope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

YES
2x2 is good, but film is still better for some things. I just wrote a
proposal for a 'scope with both.
David


On Jun 28, 2005, at 2:23 PM, John J. Bozzola wrote:

}
}
} -----------------------------------------------------------------------
} -------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} -----------------------------------------------------------------------
} --------
}
} Theoretical Question: if you were purchasing a TEM equipped with a
} high quality (2k x 2k) digital camera would you still opt for the film
} camera as well? Just wondering.
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} ##############################################################
}



From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 19:09:57 2005



From: Barbara Foster :      bfoster-at-mme1.com
Date: Tue, 28 Jun 2005 19:09:02 -0500
Subject: [Microscopy] Re: Re: Congo red & protein fibrils?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Tobias,

Well, you've got most of this right, but not entirely.

Your procedure is correct: use just the polarizer in fixed position and rotate the sample. (Actually, either could be fixed, so if you don't have a rotating stage, you can leave the sample in place and rotate a polarizing filter over the light port).

The sample doesn't really act as the second polarizer (analyzer). This is the classic experiment for either dichroism (materials which exhibit two different refractive indices) and pleochroism (materials which exhibit 3 different refractive indices).

First, a few definitions:
Refractive index is an optical property which expresses the relative interaction between the electric field in light and the electric field in matter. The stronger the interaction, the more light passing through a material will slow down and the higher the refractive index. (Mathematically, RI = velocity of light in air or vacuum/velocity of light as it interacts with a material where V air = 300,000 km/sec).

If you dissect the word "birefringence", you can see that it refers to materials
* having the property of ("gence")
* two (bi)
* refractive indices (ref in ).
In actuality, materials can have three different RI's, all at right angles to each other, but will only exhibit 2 at a time (think of either films, fibers, or crystal faces), so we never refer to them as "tri-refringent".

Polarized light is different from ordinary light in the following way:
All light has a direction of travel. You see things in the world around you because light is traveling from them to you. Light is electromagnetic radiation. As microscopists, we are most typically interested in the electrical field part (yes, I know that there have been magnetic microscopes built). The sine wave we use to describe light is actually the tracing of the tip of that electrical vector, building up then dropping off in a positive direction then building up and dropping off in negative direction. The motion of that sine wave gives light a direction of vibration. The direction of vibration is always at right angles to the direction of travel.

Ordinary light contains all directions of vibration (imagine little vectors vibrating N-S, E-W, and all angles in between; all at right angles to the direction of travel). To convert ordinary light to polarized light, you simply have to impose some sort of interaction (reflection, specific types of absorption, beam splitting) which absorbs all but one permitted direction of vibration. The result is "Plane Polarized Light", which is not necessarily always the same as linearly polarized light (tune in another time for the discussion of "States of Polarization"). However, for the sake of simplicity, plane polarized light does indeed vibrate linearly.

So what happens when you rotate any birefringent material over a polarizer? I can't draw diagrams for you here, but imagine a rectangle with one RI oriented along the short edge and the other along the long edge. As you rotate the sample, the short edge will eventually align with the permitted direction of the light coming from the polarizer. In this position, the light only "sees" one refractive index (not the most scientific explanation, but accurate). Rotating 90 degrees presents the other RI. Anywhere in between, contributions from each of the RI's will be visible. In practice, we use these unique positions to isolate each of the RI's to actually measure them.

And what's the story with things that are dichroic or pleochroic? In addition to being birefringent, they are also colored in normal illumination (ex: Congo red). To properly observe them, first set up Koehler illumination and just observe the normal color. Then, insert just a single polarizer and rotate. The colors that you see will not be the typical magentas, golds, and turquoises characteristic of polarized light interactions. Rather they will be absorption colors (browns, reds, yellows, etc.), derived from the unique property that, for these materials, not only does refractive index vary with direction, absorption does too. The result - changes in color and intensity. ... and yes, one of these directions can be very subtle.

In normal polarized light analyses, the next step would be to insert the analyzer and rotate. It is also important to note that the normal Polarization colors may be affected by the absorption colors. (For those of you who are interested, I have a "polarized light road map" which outlines all these test in flow-chart form).

Here are some interesting examples of materials which are dichroic or pleochroic:
Everyone cites tourmaline which is dark brown in one orientation and pale yellow in another,. as well as biotite (a type of mica) and cordierite.
Hartshorne and Stuart (Crystals and the Polarizing Microscope, Arnold, 1970) cite magnesium platino-cyanide which oscillates between bluish-red (parallel to "c" axis) and carmine red; hypersthene (ferro-magnesium silicate): brownish red to green; remind us that some biological materials also exhibit this property.
Patzett (Polarized Light Microscopy: Principles, Instruments, Applications, Leitz - now out of print) reminds that the phenomenon is widespread in organic materials and is responsible for the circular dichroism we chemists routinely test for in levo and dextro rotatory compounds.
And finally, I still have fond memories of doing experiments in grad school from Chamot & Mason (Handbook of Chemical Microscopy, Vol. I - now available through McCrone Associates) on a slew of materials. (For those of you who are teaching, they recommend viscose rayon dyed with congo red; crystals of o-nitrophenol, azobenzene, iodoquinine sulphate, silver chromate, copper acetate, red ammonium picrate and the magnesium platinocyanide - mentioned above).

Well, we are back at Congo red, so I'll stop here. For those of you interested in the physics of this process, I encourage you to read Hartshorne and Stuart further. For the rest of you, find some dichroic or pleochroic materials and just have fun.

After many years of teaching, I realized that all the beauty and complexity of polarized light boils down to one simple underlying concept: refractive index. And here it is again.

Hope this is helpful.
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call us at (972)954-8011.

At 05:15 PM 6/28/2005, Tobias Baskin wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
more specific without knowing which way the fibers go and how the congo binds to them).
}
} You get green presumably becaue the congo red absorbs red and blue better and so more green leaks through. Actually, polarizing filters themselves are often made by aligning really strong crystals that have dichroic abosorption, and they too have a green cast because it is difficult to have no wavelength dependence to the dichroism.
}
} Keep in mind that congo red stained fibers are going to be a whole heck of a lot less good at being a polarizing filter than a real polarizing filter, so the green signal may be pretty faint. But its tell tale appearance and disappearence at +/- 90 gives you a story. You can play with using green light (or other wave bands) but I think one's eyes are most senstivite to seeing differences in color rather than intensity.
}
} Hope this helps,
} Tobias
} }
} }
} } I am told by someone who visits my lab that protein fibrils treated with
} } congo red will show an apple green birefringence in crossed polars.
} }
} } She has brought samples for us to look at, but we cannot see the green
} } birefringence. She 'knows' there are fibrils present by another type of
} } assay.
} }
} } Has anybody heard of this technique? I think I can see the stained fibrils,
} } but nothing shows with the crossed polars. Maybe I don't know what I should
} } be looking for, maybe it is very subtle. Maybe it doesn't really work.
} }
} } If you have heard of this technique and maybe know a trick or two, let me
} } know so I can help this person.
} }
} } Thanks
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
}
}
} --
} _ ____ __ ____
} / \ / / \ / \ \ Tobias I. Baskin
} / / / / \ \ \ Biology Department
} /_ / __ /__ \ \ \__ 611 N. Pleasant St.
} / / / \ \ \ University of Massachusetts
} / / / \ \ \ Amherst, MA, 01003
} / / ___ / \ \__/ \ ____
} http://www.bio.umass.edu/biology/baskin/
} Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243


At 05:15 PM 6/28/2005, Tobias Baskin wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
more specific without knowing which way the fibers go and how the congo binds to them).
}
} You get green presumably becaue the congo red absorbs red and blue better and so more green leaks through. Actually, polarizing filters themselves are often made by aligning really strong crystals that have dichroic abosorption, and they too have a green cast because it is difficult to have no wavelength dependence to the dichroism.
}
} Keep in mind that congo red stained fibers are going to be a whole heck of a lot less good at being a polarizing filter than a real polarizing filter, so the green signal may be pretty faint. But its tell tale appearance and disappearence at +/- 90 gives you a story. You can play with using green light (or other wave bands) but I think one's eyes are most senstivite to seeing differences in color rather than intensity.
}
} Hope this helps,
} Tobias
} }
} }
} } I am told by someone who visits my lab that protein fibrils treated with
} } congo red will show an apple green birefringence in crossed polars.
} }
} } She has brought samples for us to look at, but we cannot see the green
} } birefringence. She 'knows' there are fibrils present by another type of
} } assay.
} }
} } Has anybody heard of this technique? I think I can see the stained fibrils,
} } but nothing shows with the crossed polars. Maybe I don't know what I should
} } be looking for, maybe it is very subtle. Maybe it doesn't really work.
} }
} } If you have heard of this technique and maybe know a trick or two, let me
} } know so I can help this person.
} }
} } Thanks
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
}
}
} --
} _ ____ __ ____
} / \ / / \ / \ \ Tobias I. Baskin
} / / / / \ \ \ Biology Department
} /_ / __ /__ \ \ \__ 611 N. Pleasant St.
} / / / \ \ \ University of Massachusetts
} / / / \ \ \ Amherst, MA, 01003
} / / ___ / \ \__/ \ ____
} http://www.bio.umass.edu/biology/baskin/
} Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243




From MicroscopyL-request-at-ns.microscopy.com Tue Jun 28 20:40:06 2005



From: Alan Stone :      as-at-astonmet.com
Date: Tue, 28 Jun 2005 20:39:23 -0500
Subject: [Microscopy] Re: Re: EDS Detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ouch,

Sorry for your pain. Certainly a bad situation.

Ours is a happier story...........
We purchased a Link detector some fifteen years. It has Be, thin window
and windowless positions. I am sure it is considered obsolete now, but
having the turret allows us to easily replace the thin window in the field.
Also, I believe having the permeable window positions allows us "pump down
in situ" as our LN consumption seems about the same as when it was new.
Other than very occasionally allowing the LN to evaporate out and cleaning
out any ice, the detector has never been serviced.

We did have some early problems with the AN10000 electronics. Link's
response was that we should buy a whole new system. Our in-house tech found
a blown fuse which was undocumented fuse and well hidden beneath a circuit
board.

We have since replaced all of the electronics and software with a WinEDS
system, but we will hold onto this detector as long as it keeps chugging along.



Alan Stone
ASTON






At 07:05 PM 6/28/2005, you wrote:


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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 08:27:08 2005



From: Bob Grassucci :      bob.grassucci-at-wadsworth.org
Date: Wed, 29 Jun 2005 09:29:19 -0400
Subject: [Microscopy] RE: tecnai version 2.1.5

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Roman,
The V7 hardware button you are referring to is a switch which not
only closes V7/V4 but disables the valves. If you try to open the valve
after pressing the button the HT unfortunately shuts down trying to protect
itself since the valve has been disabled for some reason. One must press
this button again to re-enable the valve before you will be able to open
it. Unfortunately there is no way to know whether you are disabled or not
and we have had similar problems when changing cameras and a new user
accidentally presses the button which on our F20 and Polara are in close
proximity. These saftey buttons are in place in case the PC dies and the
valves need to be shut directly. There is a similar arrangement with the
HT as far as I understand but at least that button lights up when it is
enabled.
Regards,
Bob

At 09:46 AM 6/29/2005 +0200, Koning, R.I. \(MCB\) wrote:
} Dear all,
}
} I am sorry to hear about your tip. Thia happening is always the biggest
} fear of a FEG user (after floodings and fires). I must say that we for
} safety reasons only open the FEG valve when we look at the specimen. In
} any other situation the lock is closed.
}
} We experienced major problems and many bugs after upgrading our TECNAI FEG
} 20 software to version 2.1.5. The most important being not to be able to
} get the negatives exposed in low dose. It appeared that having the plate
} number counter and/or exposure number in the window of our TUI created
} major communication problems with the TEM server. This resulted in a
} multitude of (irreproducable) bugs and mistakes. Getting rid of the plate
} number counter and exposure number corrected for all of these problems.
}
} Not related to this, it also appeared that the hardware button to close V7
} (which I normally never use but for educational purposes did once) not
} only closed V7 but also shut down the HT. Something that a FEI engeneer
} experienced before.
}
} With kind regards,
}
} Roman
}
} R.I.Koning (Roman) Ph.D.
} Leiden University Medical Center
} Department of Molecular Cell Biology
} Center for Electron Microscopy
} Wassenaarseweg 72 2333 AL
} P.O.Box 9503 2300 RA
} Leiden
} The Netherlands
} tel. (+31) 71 527 6463
} fax. (+31) 71 527 6440
} r.i.koning-at-lumc.nl
}
}
} -----Original Message-----
} From: owner-tecnai-at-wadsworth.org [mailto:owner-tecnai-at-wadsworth.org]On
} Behalf Of Angel M Paredes
} Sent: Tuesday, June 28, 2005 20:40
} To: Bill Tivol
} Cc: microscopy-at-msa.microscopy.com; tecnai-at-wadsworth.org; 3dem-at-ucsd.edu
} Subject: [Microscopy] tecnai version 2.1.5
}
}
} Hi all,
}
} Sunday we experienced a rather bad malfunction that people should be aware
} of that destroyed our FEG tip. On our Polara, the user working with the
} scope decided to remove the MSC to exchange the specimen. To do this you
} have to vent the airlock. He pushed the "vent airlock" button. Since he
} had not closed the column valves, the software I believe was supposed to
} have detected this and closed the column valves for him to protect the
} FEG. The software unfortunately did not. What the user did not know was
} that there was an electrical short in valve B that told the software that
} valve B was closed when it was in fact opened. Instead of telling him to
} close valve B as it should have done, the software detected that valve B
} was already closed and told the user instead to close valve A... which he
} did. The software then vented the airlock but with valve B opened instead
} of closed, the column was vented and since the software had not closed the
} column valves when the user pushed "vent airlock", the FEG was vented too.
} FEG tip gone.
}
} We are currently using version 2.1.5 of the user interface. Has anyone
} out there been experiencing any other bugs with 2.1.5? Thorsten had
} problems with his system rebooting when a log file was full and I went
} through the same thing during a bugcheck the system decided to perform.
} Another bug we've seen is in low dose. We use diffraction while in
} search. Every now and again the search mode goes from looking normal to
} looking really strange and becoming very difficult to align. When I go
} from diffraction back to regular image mode in search, I find that the
} microscope has gone from a magnification of 52,000x to 52x while in
} diffraction and this is the reason why the search mode goes from looking
} okay to looking strange. FEI's response is that we are not supposed to be
} using diffraction and experiments in the factory have shown that if one
} switches in and out of diffraction too quickly, it causes the mag to
} change. All I know is that version 2.1.3 did not have this problem. Are
} there any other problems I should be made aware of?
}
} Thanks,
} angel

********************************
Robert Grassucci
Howard Hughes Medical Institute
Wadsworth Center
Empire State Plaza
Albany, NY 12201-0509

bobg-at-wadsworth.org
Phone: (518)474-5821
Fax: (518)486-2191


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 08:50:09 2005



From: Tobias Baskin :      baskin-at-bio.umass.edu
Date: Wed, 29 Jun 2005 09:49:09 -0400
Subject: [Microscopy] Re: Re: Congo red & protein fibrils?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Barbara and group,
This is a complex area and I am far from optical
crystallagrapher (!). But there are a couple of things I want to
say, based on what I learned in school.

Birefringence describes a material that has two (or three) refractive
indices. In contrast, dichroism describes a material that has two (or
three) different absorption behaviors. (I guess we don't call them
absorption indecies).

It is true that the aligned congo red molecules on the sample would
probably also be birefringent, but this would presumably be so weak
that unless the user has a really sensitive microscope, he or she
would be unlikely to be able to detect it. In contrast, it is a lot
easer to detect differences in light intensity and color, so there is
a chance to pick up the dichroic absorption. And the way you do that
is to illuminate the sample with linearly polarized light and rotate
the sample (you are correct, you can rotate the polarizer instead)
without any analyzer, and look for the changes in color as a function
of the polarized light angle.

Now it is true that you can also add an analyzer and with cryastals
this gives you a chance to see further and different (and beautiful)
colors as the analyzer is rotated. It might increase the contrast of
a weak signal so it might be worth trying for the congo stained
protein fibers. What you would need to do in that case would be to
uncross the polars by a certain amount, say 5 degrees, rotate the
sample through at least 90, then uncross by 5 more degrees, rotate
the sample, and so on.

As ever,
Tobias



} Hi, Tobias,
}
} You've got most of this right, but not entirely.
}
} Your procedure is right: use just the polarizer in fixed position
} and rotate the sample. (Actually, either could be fixed, so if you
} don't have a rotating stage, you can leave the sample in place and
} rotate a polarizing filter over the light port).
}
} The sample doesn't really act as the second polarizer (analyzer).
} This is the classic experiment for either dichroism (materials which
} exhibit two different refractive indices) and pleochroism (materials
} which exhibit 3 different refractive indices).
}
} First, a few definitions:
} Refractive index is an optical property which expresses the relative
} interaction between the electric field in light and the electric
} field in matter. The stronger the interaction, the more light
} passing through a material will slow down and the higher the
} refractive index. (Mathematically, RI = velocity of light in air or
} vacuum/velocity of light as it interacts with a material where V air
} = 300,000 km/sec).
}
} If you dissect the word "birefringence", you can see that it refers
} to materials
}
} having the property of ("gence")
} two (bi)
} refractive indices (ref in ).
} In actuality, materials can have three different RI's, all at right
} angles to each other, but will only exhibit 2 at a time (think of
} either films, fibers, or crystal faces), so we never refer to them
} as "tri-refringent".
}
} Polarized light is different from ordinary light in the following way:
} All light has a direction of travel. You see things in the world
} around you because light is traveling from them to you. Light is
} electromagnetic radiation. As microscopists, we are most typically
} interested in the electrical field part (yes, I know that there have
} been magnetic microscopes built). The sine wave we use to describe
} light is actually the tracing of the tip of that electrical vector,
} building up then dropping off in a positive direction then building
} up and dropping off in negative direction. The motion of that sine
} wave gives light a direction of vibration. The direction of
} vibration is always at right angles to the direction of travel.
}
} Ordinary light contains all directions of vibration (imagine little
} vectors vibrating N-S, E-W, and all angles in between; all at right
} angles to the direction of travel). To convert ordinary light to
} polarized light, you simply have to impose some sort of interaction
} (reflection, specific types of absorption, beam splitting) which
} absorbs all but one permitted direction of vibration. The result is
} "Plane Polarized Light", which is not necessarily always the same as
} linearly polarized light (tune in another time for the discussion of
} "States of Polarization"). However, for the sake of simplicity,
} plane polarized light does indeed vibrate linearly.
}
} So what happens when you rotate any birefringent material over a
} polarizer? I can't draw diagrams for you here, but imagine a
} rectangle with one RI oriented along the short edge and the other
} along the long edge. As you rotate the sample, the short edge will
} eventually align with the permitted direction of the light coming
} from the polarizer. In this position, the light only "sees" one
} refractive index (not the most scientific explanation, but
} accurate). Rotating 90 degrees presents the other RI. Anywhere in
} between, contributions from each of the RI's will be visible. In
} practice, we use these unique positions to isolate each of the RI's
} to actually measure them.
}
} And what's the story with things that are dichroic or pleochroic?
} In addition to being birefringent, they are also colored in normal
} illumination (ex: Congo red). To properly observe them, first set
} up Koehler illumination and just observe the normal color. Then,
} insert just a single polarizer and rotate. The colors that you see
} will not be the typical magentas, golds, and turquoises
} characteristic of polarized light interactions. Rather they will be
} absorption colors (browns, reds, yellows, etc.), derived from the
} unique property that, for these materials, not only does refractive
} index vary with direction, absorption does too. The result -
} changes in color and intensity.
}
} The next step would be to insert the analyzer and rotate. It is
} also important to note that the normal Polarization colors may be
} affected by the absorption colors. (For those of you who are
} interested, I have a "polarized light road map" which outlines all
} these test in flow-chart form).
}
} Here are some interesting examples of materials which are dichroic
} or pleochroic:
} Everyone cites tourmaline which is dark brown in one orientation and
} pale yellow in another,. as well as biotite (a type of mica) and
} cordierite.
} Hartshorne and Stuart (Crystals and the Polarizing Microscope,
} Arnold, 1970) cite magnesium platino-cyanide which oscillates
} between bluish-red (parallel to "c" axis) and carmine red;
} hypersthene (ferro-magnesium silicate): brownish red to green;
} remind us that some biological materials also exhibit this property.
} Patzett (Polarized Light Microscopy: Principles, Instruments,
} Applications, Leitz - now out of print) reminds that the phenomenon
} is widespread in organic materials and is responsible for the
} circular dichroism we chemists routinely test for in levo and dextro
} rotatory compounds.
} And finally, I still have fond memories of doing experiments in grad
} school from Chamot & Mason (Handbook of Chemical Microscopy, Vol. I
} - now available through McCrone Associates) on a slew of materials.
} (For those of you who are teaching, they recommend viscose rayon
} dyed with congo red; crystals of o-nitrophenol, azobenzene,
} iodoquinine sulphate, silver chromate, copper acetate, red ammonium
} picrate and the magnesium platinocyanide - mentioned above).
}
} Well, we are back at Congo red, so I'll stop here. For those of you
} interested in the physics of this process, I encourage you to read
} Hartshorne and Stuart further. For the rest of you, find some
} dichroic or pleochroic materials and just have fun.
}
} After many years of teaching, I realized that all the beauty and
} complexity of polarized light boils down to one simple underlying
} concept: refractive index. And here it is again.
}
} Hope this is helpful.
} Barbara Foster
}
} Microscopy/Microscopy Education
} 313 S Jupiter Rd, Suite 100
} Allen, TX 75002
} P: 972-954-8011
} W: www.MicroscopyEducation.com
}
} P. S.
} Need a good general reference or light microscopy text? Call us
} today to learn more about "Optimizing LIght Microscopy". Copies
} still available through MME... even for class-room lots ... and we
} give quantity discounts. Call us at (972)954-8011.
}
}
}
}
}
}
} The colors exhibited are absorption colors and differ considerably
} from those seen between crossed polars (the result of the
} birefringenc
}
}
}
} At 05:15 PM 6/28/2005, Tobias Baskin wrote:
}
} } ------------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 08:53:34 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 29 Jun 2005 08:52:52 -0500
Subject: [Microscopy] Re: Film vs. digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Although 90+ percent of our needs could be met by good quality digital
imaging, I would still retain film capability for reasons that often
come up in these discussions. For important or irreplaceable images:

1) Film is archival, if processed correctly;
2) Film is platform-independent;
3) Film doesn't care if you have a CD, DVD, Zip, 3.5" floppy, 5.25"
floppy, MO drive, etc., etc., etc. (i.e., it archives itself and doesn't
need to be rearchived in newer formats as they come around);
4) Film can always be scanned into whatever format you need, hardware-
or software-wise;
5) Film is still the highest-quality imaging medium available, and;
6) Film is still relatively low in cost (and you don't HAVE to print it
unless you want to).

On the other hand:

1) Who knows how long film will be available? (Kodak has just announced
that it will stop making photo paper! Talk about the end of an era!);
2) It is a pain in the derriere to maintain film processing facilities
in labs with limited space;
3) New developments in digital imaging could catch up with films
resolution in the not-too-distant future (although upgrading to that
would certainly be pricey);
4) You might add film capability and no one will use it after all.

In my opinion, we are still a few years away from film being completely
obsolete. As Mama Tindall says, "It's better to have it and not need
it, than need it and not have it."

Now the next question is: should TEM manufacturers do away with
traditional viewing screens and go entirely to monitors?

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu








From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 09:14:48 2005



From: bbandli :      bbandli-at-mvainc.com
Date: Wed, 29 Jun 2005 10:16:00 -0400
Subject: [Microscopy] Carbon Adhesive Tabs Note

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,


Just a quick note for those using the new EMS "Ultra Smooth Carbon
Adhesive Tabs".

The surface of the tabs is much improved and very smooth and the
conductivity of the tabs is also improved, there is apparently a nickel
rich wire mesh support/substrate embedded in these tabs. The ends of
the wires are usually only visible at the very edge of the tabs and do
not appear in the area where a sample would be affixed and nickel does
not show up in an EDS spectrum of the sample collection area, many of
you may have noticed this.

I have encountered a problem when trying to lift particles off of
surfaces using these tabs. When pressure is applied to the tab while
collecting particles forces some of the wire to the surface in the
sample collection area. For most applications this isn't a problem, but
if you are analyzing a group of particles and are interested in metals
this could pose a significant problem.

Sincerely
Bryan Bandli
MVA Scientific Consultants

Disclaimer: I have no interest, financial or otherwise, in the product
mentioned or any competitors.


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 09:17:10 2005



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 29 Jun 2005 10:14:45 -0400
Subject: [Microscopy] Job Annoucement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Microscopy Facility Supervisor. Biology Department, Central Michigan
University.

Established in 1892, Central Michigan University has a growing enrollment of
approximately 28,000 students, including 19,800 students on the university's
main campus. Recently classified by the Carnegie Foundation as a
doctoral/research-intensive university, CMU is recognized for strong
undergraduate education and a range of focused graduate and research
programs. CMU is a student-focused university with opportunities for leaders
and involvement for an energetic team.

The Microscopy technician is responsible for supervising the
teaching/research microscopy facility including a transmission electron
microscope, scanning electron microscope, confocal microscope, specimen
preparation lab, and photographic darkroom. Responsibilities: teach and
assist in teaching microscopy courses, support faculty and student research,
solicit externally funded contracts, and perform routine maintenance on all
microscopy equipment and departmental light microscopes.

The position requires a Bachelor's degree or equivalent; two years
qualifying work experience; excellent organizational and communication
skills; ability to teach principles and techniques of microscopy; working
knowledge of the principles and techniques of transmission and scanning
electron microscopy; and experience with routine maintenance of EM, optical
microscopes.

Desired qualifications include a Master's degree, experience with confocal
microscopy, EDS, digital imaging, light microscopy, ultramicrotomy, vacuum
evaporation, critical point drying, and biological specimen preparation
preferred. Experience with equipment associated with the microscopy facility
preferred.

Applicants must apply online at www.jobs.cmich.edu

CMU, an AA/EO institution, strongly and actively strives to increase
diversity and provide equal opportunity within its community. CMU does not
discriminate in employment against persons based on age, color, disability,
gender, familial status, height, marital status, national origin, political
persuasion, race, religion, sexual orientation, veteran status, or weight
(www.cmich.edu/aaeo/)

Geoff Williams
Microscopy Facility Supervisor
 
Central Michigan University Biology Department Microscopy Facility
http://www.cst.cmich.edu/centers/microscopy/





From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 09:41:58 2005



From: Bob Grassucci :      bob.grassucci-at-wadsworth.org
Date: Wed, 29 Jun 2005 10:56:59 -0400
Subject: [Microscopy] Re: Re: Film vs. digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Now the next question is: should TEM manufacturers do away with
} traditional viewing screens and go entirely to monitors?

I am afraid so. Even airplane manufactures are considering replacement of
cockpit and passenger cabin windows with monitors... On the other hand,
virtual controls work just fine - such as desktop and mouse. But I would
keep a peep hole, just in case. Especially in the cockpit :-)

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com


----- Original Message -----
} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
To: {microscopy-at-microscopy.com}
Sent: Wednesday, June 29, 2005 9:52 AM

Randy,
You bring up an interesting point at the end of your message. I
believe that some of the manufacturers are indeed thinking about if not
already eliminated the projection chamber all together. I believe one of
the reasons was that with all of the new things that can be added to the
column i.e. in column energy filters, Cs correctors etc.. the column was
getting to tall to fit into most rooms.
To get back on point with the film vs digital discussion I can
only relate the experience in the structural biology community where there
are some labs that have switched to digital with 4K ccd cameras
(expensive). For practical reasons most of us are still using the tried
and true film to collect our images for high resolution ( {1.0 nm) data
collection of beam sensitive frozen hydrated samples. It gives more real
estate per shot at a small pixel size but the jury is still out with the
all digital crowd. These are just a few thoughts from someone who has been
developing film and scanning for 20 years.
Regards,
Bob

At 08:52 AM 6/29/2005 -0500, Tindall, Randy D. wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

********************************
Robert Grassucci
Howard Hughes Medical Institute
Wadsworth Center
Empire State Plaza
Albany, NY 12201-0509

bobg-at-wadsworth.org
Phone: (518)474-5821
Fax: (518)486-2191


From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 10:20:57 2005



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 29 Jun 2005 17:15:59 +0200
Subject: [Microscopy] Re: Re: Re: Film vs. digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bob Grassucci wrote

} You bring up an interesting point at the end of your message. I
} believe that some of the manufacturers are indeed thinking about if not
} already eliminated the projection chamber all together. I believe one of
} the reasons was that with all of the new things that can be added to the
} column i.e. in column energy filters, Cs correctors etc.. the column was
} getting to tall to fit into most rooms.

It's done !
Have a look at :

http://www.jeol.com/tem_/temprods/jem2200fs.html


Jacques Faerber



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 10:45:36 2005



From: Christopher Gilpin :      christopher.gilpin-at-utsouthwestern.edu
Date: Wed, 29 Jun 2005 10:44:50 -0500
Subject: [Microscopy] Re: Re: Re: Film vs. digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,
I have a JEOL 2200FS. Indeed there is no viewing chamber in the conventional
sense. There is a small viewing screen hidden away for alignment purposes
only. Imaging is carried out solely with cameras.


Chris



Christopher J Gilpin Ph.D.
Assistant Professor
Director, Molecular and Cellular Imaging Facility
K1.A04
UT Southwestern Medical Center at Dallas
5323 Harry Hines Boulevard
Dallas, TX 75390-9039
Phone +1 214 648 2827
Fax +1 214 648 6408

-----Original Message-----
} From: Faerber Jacques [mailto:Jacques.Faerber-at-ipcms.u-strasbg.fr]
Sent: Wednesday, June 29, 2005 10:16 AM
To: Microscopy Society of America


Bob Grassucci wrote

} You bring up an interesting point at the end of your message.
} I believe that some of the manufacturers are indeed thinking about if
} not already eliminated the projection chamber all together. I believe
} one of the reasons was that with all of the new things that can be
} added to the column i.e. in column energy filters, Cs correctors etc..
} the column was getting to tall to fit into most rooms.

It's done !
Have a look at :

http://www.jeol.com/tem_/temprods/jem2200fs.html


Jacques Faerber




From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 11:59:23 2005



From: John.Catino-at-mineralstech.com
Date: Wed, 29 Jun 2005 12:58:36 -0400
Subject: [Microscopy] Re: ultra-microtomy of water soluable crystals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





I've have similar problems with samples only slightly soluble in water. To
overcome dissolution, I saturate the water with the compound. In my case,
it only takes 1-2 mg of material.
Seem to work great. No more Swiss cheese!


John W. Catino
Analytical Investigator - Microscopy
Minerals Technologies, Inc.
640 N. 13th Street
Easton, PA 18042

Tel: 610.250.3363
Fax: 610.250.3206
john.catino-at-mineralstech.com



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From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 12:13:34 2005



From: Anthony Garratt-Reed :      tonygr-at-MIT.EDU
Date: Wed, 29 Jun 2005 13:11:01 -0400
Subject: [Microscopy] Re: EDS Detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a Rontec X-Flash detector on a JEOL 5910 SEM. This is a
silicon-drift detector which operates at -15C using simple thermoelectric
cooling. I am very satisfied with it for our application, which is
qualitative microanalysis and x-ray mapping. I can't speak for
quantitative analysis, as we almost never attempt it in this system. The
resolution and baseline tend to change more with countrate than with a
Si(Li), which might make high-precision quantitative analysis more
problematic, but I expect the electronics will improve, too, and help
compensate. It does not detect the light elements (C-N-O) at higher
countrates, but sees them fine at 1000cps. I would consider a SDD very
seriously for a future application, but I'm not convinced yet that it would
replace a Si(Li) in every case.

I have an EDAX Sapphire Si(Li) detector with a small (angular) dewar. The
concept made sense, that one filled it up only when needed for an
occasional analysis. The reality depends on the meaning of
"occasional". The SEM it is on is very busy with imaging, and EBSD, but
users use EDS once or twice a week. It is a pain to have to interrupt the
other work on the SEM to chill the detector two hours before you need to
use it! The detector works exactly as intended - I'm not criticizing EDAX,
it was just the wrong decision for our application.

I have 3 Oxfords, of varying ages, including a windowless on the VG STEM,
which was built in 1991 and has never yet had to go for repair and which
still has a resolution at Mn of {135eV (using a modern pulse processor)
compared with the original specification of 138eV. This detector has run
with two preamplifiers, on an AN10000, an eXL Mk. 1, two Isis systems, a
(short-lived) Emispec ES Vision 4, and currently an INCA. Quite a history!

Tony.


At 10:30 AM 6/28/2005, Tom W Bargar wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************



From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 13:37:28 2005



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 29 Jun 2005 15:14:51 -0400
Subject: [Microscopy] Re: Re: ultra-microtomy of water soluable crystals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

That's a good idea but it may not deal with all the problems since the presence of
moisture may allow recrystallization to take place, resulting in the substitution
of one polymorph for another, even if there is no net loss of material.

John Twilley

John.Catino-at-mineralstech.com wrote:

} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} I've have similar problems with samples only slightly soluble in water. To
} overcome dissolution, I saturate the water with the compound. In my case,
} it only takes 1-2 mg of material.
} Seem to work great. No more Swiss cheese!
}
} John W. Catino
} Analytical Investigator - Microscopy
} Minerals Technologies, Inc.
} 640 N. 13th Street
} Easton, PA 18042
}
} Tel: 610.250.3363
} Fax: 610.250.3206
} john.catino-at-mineralstech.com
}
} **********************************************************************
} This email and any files transmitted with it are confidential and
} intended solely for the use of the individual or entity to whom they
} are addressed. If you have received this email in error please notify
} the system manager.
}
} This footnote also confirms that this email message has been swept by
} MIMEsweeper for the presence of computer viruses.
} www.mimesweeper.com
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From MicroscopyL-request-at-ns.microscopy.com Wed Jun 29 14:15:26 2005



From: Patrick Goodwill :      goodwill-at-berkeley.edu
Date: Wed, 29 Jun 2005 12:14:46 -0700
Subject: [Microscopy] High Frequency Acoustic Microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

I'm looking for a high frequency acoustic microscope (200+ Mhz) in
pretty much any university or government lab in or near the san
francisco bay area that I might be able to bargain some time on. At
a minimum, this includes UC Berkeley, UC Davis, UC Santa Cruz, UC SF,
Stanford, LBNL, and LLNL. Any idea where I might find one?
Unfortunately, the original Stanford group that pioneered this work
no longer studies or uses acoustic microscopy.

I'm trying to characterize the mechanical properties of small cells
approx 10um in diameter (size, impedance, elasticity, etc).

Patrick.
----
Graduate Student
UCSF/UC Berkeley Joint Graduate Group in Bioengineering
408-203-6052





From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 04:40:45 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Thu, 30 Jun 2005 07:09:12 -0230
Subject: [Microscopy] RE: Re: EDS Detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anthony Garratt-Reed writes ...

} I have a Rontec X-Flash detector on a JEOL 5910 SEM. ...

We too have recently installed a Roentec, and we are absolutely amoazed at
its speed. We measure Mn metal the other day at 150kcps (into the spectrum)
at Mn Ka FWHM=165eV! However, our applications are mineral mapping. Like
Dr Garratt-Reed, we would also believe with the addition of this detector on
the market, potential purchasers should consider their applications relative
to "quantitative analysis" versus "image or spatial analysis", and don't
forget to test drive the software.

cheerios ... Michael Shaffer :o)
Avalon Peninsula, Newfoundland



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 08:00:25 2005



From: exploratorium-at-tiscali.it (by way of MicroscopyListserver)
Date: Thu, 30 Jun 2005 07:59:41 -0500
Subject: [Microscopy] viaWWW: Request for prepared microscope slides for science museum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (exploratorium-at-tiscali.it) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Tuesday, June 28, 2005 at 11:42:12
---------------------------------------------------------------------------

Email: exploratorium-at-tiscali.it
Name: giovanni de caro

Organization: museo laboratorio di scienze naturali per ragazzi "L. MontalbÚ" - casalciprano (Cb) - Molise - Italia

Title-Subject: [Microscopy] [Filtered] MListserver: Request for prepared microscope slides for science museum

Question: Hi all! I am the director of a small, volunteer operated and self funded science museum for youngsters based in southern Italy (see our website - translated in english : http://web.tiscali.it/exploratorium).
We have here a small biology lab equipped with optical microscopes; we do organize small courses of biology for kids. If you have to donate some prepared microscope slides of: vegetal tissues, protozoa, fungi, insects or other which maybe of ineterst for us, let us know; we can pay for shipping from USA or elsewhere, if requested.
We also are looking for an older, working slide projector; shipping as above.

Thank you for your kind help.

Giovanni De Caro,MD
Italia

---------------------------------------------------------------------------



From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 08:00:55 2005



From: monica.iliescu-at-polymtl.ca (by way of MicroscopyListserver)
Date: Thu, 30 Jun 2005 08:00:10 -0500
Subject: [Microscopy] viaWWW: uranyl acetate, ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (monica.iliescu-at-polymtl.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, June 28, 2005 at 12:51:56
---------------------------------------------------------------------------

Email: monica.iliescu-at-polymtl.ca
Name: Monica ILIESCU

Organization: Ecole Polytechnique

Title-Subject: [Microscopy] [Filtered] uranyl acetate, ESEM

Question: Hello,

I would like to ask if someone of you know if uranyl acetate (generaly used in transmission electron microscopy) can be used in ESEM and if it possesses a potential radioactivity risk.

Thank you in advance.

Monica

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 08:01:24 2005



From: thomas.richards-at-arkemagroup.com (by way of MicroscopyListserver)
Date: Thu, 30 Jun 2005 08:00:42 -0500
Subject: [Microscopy] viaWWW: Service for LEO 1530 FE-SEM near Philadelphia, PA.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (thomas.richards-at-arkemagroup.com) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, June 30, 2005 at 05:56:05
---------------------------------------------------------------------------

Email: thomas.richards-at-arkemagroup.com
Name: Thomas Richards

Organization: Arkema

Title-Subject: [Microscopy] [Filtered] Service for LEO 1530 FE-SEM near Philadelphia, PA.

Question: Hello,

I am looking for someone to service our LEO 1530 FE-SEM and would appreciate any help. We are located near Philadelphia, PA. Thanks.

Thomas Richards
Senior Research Chemist, Systems and Materials Analysis
Analytical and Systems Research
Arkema Inc.
900 First Avenue
King of Prussia, PA 19406
(610) 878-6309
(610) 878-6196 fax
thomas.richards-at-arkemagroup.com

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 09:09:21 2005



From: =?iso-8859-1?B?TWFyaWUtQ2xhdWRlIELpbGFuZ2Vy?= :      mcbelanger6-at-hotmail.com
Date: Thu, 30 Jun 2005 14:08:37 +0000
Subject: [Microscopy] RE: Congo red & protein fibrils?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jonathan,

In our lab, we currently use a Congo red staining for amyloid fibers in
spleen sections. We do use both polarizer and analyzer and have a very
bright specific green birefringence, provided that the intensity of light is
very high. Furthermore, we have been able to see a bright red fluorescence
in these areas. For the fluorescence, though, artefacts seem to be a
problem.

What protein are you trying to look at? does it display some kind of
alignment as beta-sheets?

Regards,

Marie-Claude Belanger
Montreal


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 09:11:44 2005



From: =?iso-8859-1?B?TWFyaWUtQ2xhdWRlIELpbGFuZ2Vy?= :      mcbelanger6-at-hotmail.com
Date: Thu, 30 Jun 2005 14:11:02 +0000
Subject: [Microscopy] Re: Congo red & protein fibrils?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jonathan,

In our lab, we currently use a Congo red staining for amyloid fibers in
spleen sections. We do use both polarizer and analyzer and have a very
bright specific green birefringence, provided that the intensity of light is
very high. Furthermore, we have been able to see a bright red fluorescence
in these areas. For the fluorescence, though, artefacts seem to be a
problem.

What protein are you trying to look at? does it display some kind of
alignment as beta-sheets?

Regards,

Marie-Claude Belanger
Montreal




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 09:35:52 2005



From: Ronald Smith :      rsmith-at-uwo.ca
Date: Thu, 30 Jun 2005 10:44:08 -0400
Subject: [Microscopy] Freeze Fracture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,
I have a student wishing to do some freeze fracture of Bacteria for
SEM. We are looking for a unit where we could have some samples
prepared, preferably in the Great Lakes area. Any suggestions would be
appreciated.


Thanks,
Ron.

Ronald J. Smith
Department of Biology
Room 235, Biological & Geological Sciences Bldg.
U.W.O., London, Ontario
N6A 5B7
(519) 661-2111 ext.86486




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 11:38:01 2005



From: Bill Tivol :      tivol-at-caltech.edu
Date: Thu, 30 Jun 2005 09:37:08 -0700
Subject: [Microscopy] Re: viaWWW: uranyl acetate, ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jun 30, 2005, at 6:00 AM, by way of MicroscopyListserver wrote:

} I would like to ask if someone of you know if uranyl acetate (generaly
} used in transmission electron microscopy) can be used in ESEM and if
} it possesses a potential radioactivity risk.
}
Dear Monica,
I don't know what you want to examine, so I don't know what use UAc
will be to you in the ESEM, but I think that you could look at a
specimen to which UAc has been added without risk to the instrument.
There is no particular problem related to the very small amount of
radioactivity in the small quantities of UAc generally used in EM;
however, you should check with your safety office about proper handling
and disposal of UAc, since the laws regarding this vary from place to
place. Since U is an alpha emitter, the radiation will not penetrate
through the dead layer of the skin, so it is a hazard only when
ingested or inhaled. Your safety office will advise you on protocols
to reduce the likelihood that you will be at risk from ingestion or
inhalation. In our lab, all use of UAc in confined to a region in a
fume hood, and anything used with UAc that is to be disposed of is
placed in a labeled container in that space and picked up by the safety
office. One should always wash ones hands after working with UAc.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu




From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 16:14:24 2005



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 30 Jun 2005 16:13:36 -0500
Subject: [Microscopy] dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are comparing dye sub printers versus the Fuji Pictrography
PG4500. The price differential is a staggering $22,000 versus $6,500,
respectively. It is my understanding that both produce good quality,
gray-scale prints (versus dithered, inkjet prints). Why the price
differential? I would welcome any comments, user experiences, etc.
Does anyone know the average cost per 8.5 x 11in print?

Thank you.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################


From MicroscopyL-request-at-ns.microscopy.com Thu Jun 30 18:04:09 2005



From: bberkmeyer-at-flood.com (by way of Ask-A-Microscopist)
Date: Thu, 30 Jun 2005 18:03:27 -0500
Subject: [Microscopy] AskAMicroscopist: microtomes on a piece of wood

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bberkmeyer-at-flood.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, June 30, 2005 at 11:38:55
---------------------------------------------------------------------------

Email: bberkmeyer-at-flood.com
Name: Brad Berkmeyer

Organization: The Flood Company

Education: Graduate College

Location: Hudson, OH

Question: What would be suggested for a microtomes on a piece of wood (pine) which has been coated with a thermoplastic polymer coating? I would probably have to freze the sample to avoid any heat generated in cutting...coating will be staned with Rhodamine 6 and viewed under UV Flour..Goal is looking at coating penetration. Also, is a microtome the correct preparation?

Thanks,

---------------------------------------------------------------------------


From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 07:14:09 2005



From: michael shaffer :      michael-at-shaffer.net
Date: Fri, 1 Jul 2005 09:43:19 -0230
Subject: [Microscopy] RE: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John J. Bozzola writes ...


} We are comparing dye sub printers [...]. It is my understanding
} that both produce good quality, gray-scale prints (versus
} dithered, inkjet prints). ...

Inkjet printers may dither, but you cannot see the dithering at all with
modern printers. For excellent color, excellent grayscale, as well as
archival prints, I would suggest you take a look at the 9-ink HP Photosmart
8750. It's only downside is a thirst for ink cartridges, but considering
the prices you are comparing, this printer would be a bargain.

Genuinely, Michael Shaffer :o)
SEM/MLA Lab Coordinator
(709) 737-6790 (Ofc)
(709) 737-6790 (Lab)
{www.micro-investigations.com}
{www.esd.mun.ca/epma/}
Inco Innovation Centre
Memorial University of Newfoundland
St. John's, Newfoundland



From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 07:22:43 2005



From: Alan Stone :      as-at-astonmet.com
Date: Fri, 01 Jul 2005 07:22:01 -0500
Subject: [Microscopy] Re: RE: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Canon i9900. The prints are outstanding and in a way, sadly
better than anything I could do in the darkroom.

Alan Stone
ASTON



At 07:13 AM 7/1/2005, you wrote:


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com



From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 08:08:20 2005



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Fri, 1 Jul 2005 08:07:37 -0500
Subject: [Microscopy] RE: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For a few years Pictrography was my printer of choice (average cost per
print
is about $2 or $3). Half a year ago I bought cheap inkjet HP Deskjet
6540
for draft prints. Surprisingly, it produces grayscale prints of very
good quality, so now
I practically stopped using Pictrography.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
} Sent: Thursday, June 30, 2005 4:14 PM
} To: Microscopy-at-msa.microscopy.com
} Subject: [Microscopy] dye sub versus Pictrography
}
}
}
}
} --------------------------------------------------------------
} ----------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} -----------------
}
} We are comparing dye sub printers versus the Fuji Pictrography
} PG4500. The price differential is a staggering $22,000 versus $6,500,
} respectively. It is my understanding that both produce good quality,
} gray-scale prints (versus dithered, inkjet prints). Why the price
} differential? I would welcome any comments, user experiences, etc.
} Does anyone know the average cost per 8.5 x 11in print?
}
} Thank you.
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} ##############################################################
}
}



From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 08:37:32 2005



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 1 Jul 2005 08:36:50 -0500
Subject: [Microscopy] Re: RE: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have to agree with the ink jet crowd. I have a Canon i9100 for
personal use which will make prints up to 19x13 inches and it also gives
me much more control than I had in the darkroom (and John Bozzola knows
what a switch it is to hear that from me---I used to live in
darkrooms!). I simply couldn't justify paying thousands of dollars for
high-end dye-sub printers or the Fuji system in our lab, when a $500-600
printer will give excellent quality prints with a life span of 100+
years, using the right inks and papers.

I honestly don't even remember when we made the last print for our users
in the lab. I bought a cheaper, but good quality ink-jet a couple of
years ago for people who wanted prints and the original ink cartridges
are still in it. We shoot film on the TEM and scan it and our SEM takes
digital images directly. We give our clients the images on disks and
they're happy. Occasionally we do quick work prints on a laser printer
upon request, but even this is rare.

I still have a darkroom and two enlargers in storage in my garage, but
it's becoming more out of nostalgia than any real hope I'll set them up
again someday. I just can't let go......

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu



-----Original Message-----
} From: Alan Stone [mailto:as-at-astonmet.com]
Sent: Friday, July 01, 2005 7:22 AM
To: microscopy-at-microscopy.com


We have a Canon i9900. The prints are outstanding and in a way, sadly
better than anything I could do in the darkroom.

Alan Stone
ASTON



At 07:13 AM 7/1/2005, you wrote:


} -----------------------------------------------------------------------
} ------- The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver

} } both produce good quality, gray-scale prints (versus dithered,
} } inkjet prints). ...
}
} Inkjet printers may dither, but you cannot see the dithering at all
} with modern printers. For excellent color, excellent grayscale, as
} well as archival prints, I would suggest you take a look at the 9-ink
} HP Photosmart 8750. It's only downside is a thirst for ink cartridges,

} but considering the prices you are comparing, this printer would be a
bargain.
}
} Genuinely, Michael Shaffer :o)
} SEM/MLA Lab Coordinator
} (709) 737-6790 (Ofc)
} (709) 737-6790 (Lab)
} {www.micro-investigations.com}
} {www.esd.mun.ca/epma/}
} Inco Innovation Centre
} Memorial University of Newfoundland
} St. John's, Newfoundland

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com





From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 09:02:28 2005



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Fri, 01 Jul 2005 10:00:36 -0400
Subject: [Microscopy] Re: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is no reason to spend this kind of money to get excellent B&W
prints. Many fine art photographers are making beautiful prints with ink
jet printers. Check out Lyson.com or inkjetmall.com. Also, continuous
inking systems are available that bypass individual ink cartridges. I
will post more specifics later or early next week.

Geoff


John J. Bozzola wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} We are comparing dye sub printers versus the Fuji Pictrography PG4500.
} The price differential is a staggering $22,000 versus $6,500,
} respectively. It is my understanding that both produce good quality,
} gray-scale prints (versus dithered, inkjet prints). Why the price
} differential? I would welcome any comments, user experiences, etc.
} Does anyone know the average cost per 8.5 x 11in print?
}
} Thank you.



--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 10:53:09 2005



From: Diane Baldwin :      dbaldwin-at-dgisrd.com
Date: Fri, 1 Jul 2005 11:52:28 -0400
Subject: [Microscopy] TEM/SEM Job Accouncement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ELECTRON MICROSCOPE TECHNICIAN - RESEARCH TRIANGLE PARK (RTP), NORTH
CAROLINA AREA



We have an immediate need for a full-time Electron Microscope Technician
(maintenance and operation). 3+ years TEM and SEM experience in
biological, polymer, carbon/carbon composite, and semi-conductor
electron microscopy required. Send resume and salary requirements to
sjeffers-at-dgisrd.com.




From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 11:15:37 2005



From: Don Chernoff at ASM :      donc-at-asmicro.com
Date: Fri, 1 Jul 2005 11:04:37 -0500
Subject: [Microscopy] Image review: Hard copy vs. PC screen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This post is tangential to the dye sub vs ink jet discussion.
When one needs to compare 2 or more images simultaneously, prints can easily
be laid out on a table, but this is not so easy with on-screen display of
digital images. One thing that we have done in our lab is to install a
second monitor at some workstations and increase the Windows desktop size so
that it spans the two monitors. This is satisfactory for AFM images where
the basic pixel count is 512x512 but may not be so good with other formats.
I am curious to learn what other people do.

When you give your customers digital images only, do you feel there is a
risk they could miss an important comparison?

regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]



From MicroscopyL-request-at-ns.microscopy.com Fri Jul 1 19:35:59 2005



From: Damian Neuberger :      neuberger1234-at-comcast.net
Date: Fri, 1 Jul 2005 19:35:18 -0500
Subject: [Microscopy] Re: dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don't forget to use RIP software for excellent tonal range. I've seen
photos done with and without and there is a discernible difference and
improvement.
Damian

-----Original Message-----
} From: Geoff McAuliffe [mailto:mcauliff-at-umdnj.edu]
Sent: Friday, July 01, 2005 9:01 AM
To: John J. Bozzola
Cc: Microscopy-at-msa.microscopy.com

There is no reason to spend this kind of money to get excellent B&W
prints. Many fine art photographers are making beautiful prints with ink
jet printers. Check out Lyson.com or inkjetmall.com. Also, continuous
inking systems are available that bypass individual ink cartridges. I
will post more specifics later or early next week.

Geoff


John J. Bozzola wrote:

} --------------------------------------------------------------------------
----
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
-----
}
}
} We are comparing dye sub printers versus the Fuji Pictrography PG4500.
} The price differential is a staggering $22,000 versus $6,500,
} respectively. It is my understanding that both produce good quality,
} gray-scale prints (versus dithered, inkjet prints). Why the price
} differential? I would welcome any comments, user experiences, etc.
} Does anyone know the average cost per 8.5 x 11in print?
}
} Thank you.



--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************







From MicroscopyL-request-at-ns.microscopy.com Sat Jul 2 02:54:09 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Sat, 2 Jul 2005 00:53:23 -0700
Subject: [Microscopy] Image review: Hard copy vs. PC screen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don;
I tried to purchase an IBM T-220 9 megapixel display, but was
told by IBM that they had killed the product. This was the only display
ever marketed that could display a 2K X 2K camera image showing all of
the pixels on one screen. We still have two screens on our TEM, but need
to either display the images at half resolution, or zoom to display only
part of the image.

John Mardinly

-----Original Message-----
} From: Don Chernoff at ASM [mailto:donc-at-asmicro.com]
Sent: Friday, July 01, 2005 9:05 AM
To: Microscopy List

This post is tangential to the dye sub vs ink jet discussion.
When one needs to compare 2 or more images simultaneously, prints can
easily
be laid out on a table, but this is not so easy with on-screen display
of
digital images. One thing that we have done in our lab is to install a
second monitor at some workstations and increase the Windows desktop
size so
that it spans the two monitors. This is satisfactory for AFM images
where
the basic pixel count is 512x512 but may not be so good with other
formats.
I am curious to learn what other people do.

When you give your customers digital images only, do you feel there is a
risk they could miss an important comparison?

regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA &
Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes,
consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]





From MicroscopyL-request-at-ns.microscopy.com Sat Jul 2 03:04:41 2005



From: Mardinly, John :      john.mardinly-at-intel.com
Date: Sat, 2 Jul 2005 01:03:56 -0700
Subject: [Microscopy] dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John;
We have a Fuji Pictrograph 3500, and I have to say that nothing
I have ever seen prints with the resolution and vivid saturation of the
pictrograph, both for color and B&W. However, since all of our
conference rooms got digital projectors, we don't make prints any more.
I used it to make some absolutely beautiful prints of my daughter, but
that's about all. We have had multiple failures of a $1,000 circuit
board, and the Fuji service department is a NIGHTMARE to deal with.
Right now, the printer is inoperative. The most recent batch of paper
and donor sticks to the drum, causing a fault. I don't know if spending
another $400 for new rolls will cure that problem, or if there is
something else wrong with the printer, but we're probably going to just
push it out the door.

John Mardinly
Intel

This is the opinion of the author and not of Intel Corporation.

-----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Thursday, June 30, 2005 2:14 PM
To: Microscopy-at-msa.microscopy.com

We are comparing dye sub printers versus the Fuji Pictrography
PG4500. The price differential is a staggering $22,000 versus $6,500,
respectively. It is my understanding that both produce good quality,
gray-scale prints (versus dithered, inkjet prints). Why the price
differential? I would welcome any comments, user experiences, etc.
Does anyone know the average cost per 8.5 x 11in print?

Thank you.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################






From MicroscopyL-request-at-ns.microscopy.com Sat Jul 2 08:41:32 2005



From: =?iso-8859-2?B?TC4gS+pwafFza2k=?= :      L.Kepinski-at-int.pan.wroc.pl
Date: Mon, 4 Jul 2005 08:43:08 +0200
Subject: [Microscopy] EDAX PV9800 repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues - Two notices this message

Archives:
-------
The June archives are now on-line at:

http://www.microscopy.com/MicroscopyListserver


Viruses/Spam:
-----------

I haven't yet figured out who, but at least one of our subscribers has
a worm/virus which is infecting their computer. About once
per week it is scanning and then sending a SPAM message through the system and because
it has the embedded authorization it is getting through the spam filters.

I am trying to sort out how to block this (an who it is really coming from)
but it is a non-trival issue this time and will take me some time to sort this one out.

PLEASE... run a VIRUS scan on your computers. At least one of you has a virus
that is reading your Email boxes and using this to attach the Listserver
(and probably alot of other people too...)

Just to give you an idea of the magnitude. The Listserver filter stopped
175 junk messages on July 1, 2005. Yep we are approaching 200
trash messages/day. Only a few per month get through, but it is getting
harder all the time to catch these.

Rejected Email:
-----------

Please also remember if you inadvertently get caught by one of the SPAM filters READ
the message returned and follow the directions. I am also getting alot of
people asking why their message was rejected. Nearly all of these people do not
bother to read the explaination of the problem found and just ask "why am I being rejected".
MOST of the time is it because they have hidden attachments (usually in HTML) appended
without their knowledge by their Email programs. To avoid this make sure your settings on your Email
client are set to sent only plain/text messages and not formatted, or rich text
(bold, italic, colorized). This is what most frequently causes the attachments to
be created. If you can't turn this off then use the on-line Forms to submit
your question/comment at

http://www.microscopy.com/MicroscopyListserver


Thanks in advance...


Nestor
Your Friendly Neighborhood SysOp





From "zaluzec-at-microscopy.com" Sun Jul 3 11:22:41 2005
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Message-Id: {p0611040fbeedbe85f3a4-at-[206.69.208.22]}

Colleagues

Your may safely ignore this test mesage.

I have updated the security model and the software for the Listserver
this morning , and need to perform a full mailing test to all subscribers
to confirm functionality. Basically I believe that I have patched a few software
holes that spammers have discovered.

I will be monitoring the system for problems the rest of the day.
Hopefully this test will run fine and there will be minimal interruptions.

Should you encounter problems please contact me off-line (zaluzec-at-microscopy.com).


Cheers

Nestor
Your Friendly Neighborhood SysOp

Sunday July 3, 2005 11:20 AM CST
-----------------------------


From "vitalylazar-at-att.net" Sun Jul 3 16:03:39 2005
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Message-ID: {006e01c58012$961fa4b0$6501a8c0-at-sia719y7xok2eg}

Good day all,

Two similar models of this monitor were available: IBM T221 and Viewsonic
VP2290B, both using IBM display panel 22" diagonal, 3840 x 2400 pixels, 204
dpi. Both discontinued. IBM had intentions of replacing T221 with new model
as early as March 2005. I don't know whether they did yet.

We purchased several such monitors, IBM and Viewsonic, for TEM camera
systems. Both models are similar in performance, except Viewsonic is a bit
slower in full resolution mode, which makes no difference for static images.
It's hard for a PC to run 9.2 MP frames at over 20 FPS refresh rate anyway.
In fact, it is better to run 1600 x 1200 desktop most of the time and switch
to 3840 x 2400 for high resolution viewing. 1600 x 1200 keeps fonts and
icons size comfortable, and refresh rate is fast. The display in 3840 x 2400
mode is stunning- like looking through a window on a sunny day. You can take
an eye loupe to the screen and see further detail in the image.

If you will be able to find refurbished or second hand T221 - IBM will honor
original 3 year factory warranty as long as monitor is not physically
damaged. One of our IBM monitors was refurbished, with a screen defect. IBM
replaced the monitor. Consult with IBM regarding the warranty, before buying
used monitor. Have monitor serial number when calling IBM. These monitors
are still available, mostly on e-bay, and through some internet outlets.
Could be even new in box, but not from a regular source.

Is anybody at IBM reading this message? Please comment on a future
availability of equal or better display monitor.

See T221 in EM application at
www-1.ibm.com/industries/healthcare/doc/content/bin/ls_t221_enhancing_electr
on_1.pdf

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
} From: "Mardinly, John" {john.mardinly-at-intel.com}
To: "Don Chernoff at ASM" {donc-at-asmicro.com} ; "Microscopy List"
{microscopy-at-microscopy.com}
Sent: Saturday, July 02, 2005 3:53 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amr2w-at-virginia.edu) from http://microscopy.com/MLFormMail.html on Thursday, June 30, 2005 at 12:58:42
---------------------------------------------------------------------------

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: UVa

Title-Subject: [Microscopy] [Filtered] 3-D SEM reconstruction

Question: Hi all,
I know there are software programs that do 3-D images from SEM using stereo pairs, however, what if you have a more complex shaped object? I hear that there has been new software that you can use a series of tilts like TEM tomography to obtain a better reconstruction. Does anybody know about this? Any information would be very much appreciated. Thanks!
-Andrew Roelant

---------------------------------------------------------------------------


From "srandol3-at-utk.edu" Sun Jul 3 21:04:57 2005
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X-Sender: (Unverified)
Message-Id: {p06110402beee498888e5-at-[206.69.208.22]}

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (srandol3-at-utk.edu) from http://www.microscopy.com/MLFormMail.html on Sunday, July 3, 2005 at 19:26:02
---------------------------------------------------------------------------

Email: srandol3-at-utk.edu
Name: Steven Randolph

Organization: university of tennessee

Title-Subject: [Microscopy] [Filtered] MListserver: Area analysis scanning

Question: Hello all,

I have a question that is really more engineering-related than imaging. My question is regarding some of the various scanning modes on Hitachi SEMs such as the S4300, S4700, and S3500N. I need to know some of the specifics about how scanning takes place in area analysis mode and reduced screen mode. In area analysis, is the beam blanked in the region outside the box, or is the pixel size reduced to accomodate the box size? I guess what I'm trying to find out is the dwell time in area analysis and reduced screen mode. I seem to recall that there is a finite settle time associated with scanning in some modes. Anyways, any input you could provide would be much appreciated!

Thanks much,
Steven Randolph

---------------------------------------------------------------------------




From "L.Kepinski-at-int.pan.wroc.pl" Mon Jul 4 01:45:21 2005
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Message-ID: {001801c58063$a3b20e00$8b01a8c0-at-b8p020}

Dear all,



We have a Philips SEM 515 equipped with EDX spectrometer. The spectrometer
is EDAX PV 9800 system with UTW detector (freshly repaired) and control unit
with its specialized computer (both hardware and software). The computer is
just dying and most probably can not be repaired. I wonder if anybody tried
to "upgrade" this particular system to PC compatible version. Is it possible
at reasonable cost?

Regards,

Leszek



Leszek Kepinski
Institute of Low Temperature and Structure Research,
Polish Academy of Sciences,
P.O.Box 1410,
50-950 Wroclaw, Poland
e-mail: L.Kepinski-at-int.pan.wroc.pl







From: zaluzec-at-microscopy.com
Date: Mon, 4 Jul 2005 13:54:57 -0500
Subject: [Microscopy] Administrivia: Sorry - another full system test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues:

Well, it was wishful thinking that I could adequitely solve the problem in the
first attempt. After many hours of off-line testing (and too few pints for a holiday
weekend). I need to run yet another full system delivery test. You may notice a
few changes in the configuration of the Email headers in this version.

The previous test basically worked, but it took far too many hours to process & verify
all the addresses. I've taken a whole different aproach with this modification
(after having looked at the results over night).

No need to reply to this message. Again if you have problems posting with this
new version of the filters implements, then please contact me off-line.
(zaluzec-at-microscopy.com)

Nestor
Your Friendly Neighborhood SysOp.

Monday - July 4th, 2005




------------------------------Original Headers------------------------------
16, 11 -- From zaluzec-at-microscopy.com Mon Jul 4 13:54:57 2005
16, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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16, 11 -- Date: Mon, 4 Jul 2005 13:54:54 -0500
16, 11 -- To: microscopy-at-microscopy.com
16, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
16, 11 -- Subject: Administrivia: Sorry - another full system test
16, 11 -- Content-Type: text/plain; charset="us-ascii"
------------------------------------------------------------------------





From: zaluzec-at-microscopy.com
Date: Mon, 4 Jul 2005 18:44:25 -0500
Subject: [Microscopy] Administrivia: Sorry - another full system test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Testing new X-header 6:45 pm


------------------------------Original Headers------------------------------
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1, 11 -- Date: Mon, 4 Jul 2005 18:44:23 -0500
1, 11 -- To: microscopy-at-microscopy.com
1, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
1, 11 -- Subject: Testing new X-header 6:45 pm
1, 11 -- Content-Type: text/plain; charset="us-ascii"


------------------------------End of - Headers------------------------------




From: zaluzec-at-microscopy.com
Date: Mon, 4 Jul 2005 18:46:05 -0500
Subject: [Microscopy] Testing new X-header 6:47 pm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Testing new X-header 6:47 pm


------------------------------Original Headers------------------------------
1, 11 -- From zaluzec-at-microscopy.com Mon Jul 4 18:46:04 2005
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1, 11 -- To: microscopy-at-microscopy.com
1, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
1, 11 -- Subject: Testing new X-header 6:47 pm
1, 11 -- Content-Type: text/plain; charset="us-ascii"


------------------------------End of - Headers------------------------------




From: zaluzec-at-microscopy.com
Date: Mon, 4 Jul 2005 18:48:06 -0500
Subject: [Microscopy] Testing new X-header 6:48 pm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Testing new X-header 6:48 pm


------------------------------Original Headers------------------------------
1, 11 -- From zaluzec-at-microscopy.com Mon Jul 4 18:48:06 2005
1, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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1, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
1, 11 -- Subject: Testing new X-header 6:48 pm
1, 11 -- Content-Type: text/plain; charset="us-ascii"


------------------------------End of - Headers------------------------------




From: Stephen.Cody-at-ludwig.edu.au
Date: Mon, 4 Jul 2005 19:10:35 -0500
Subject: [Microscopy] FOM 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The next in the FOM conference series will take place in Perth, (Western) Australia from Sunday April 9th to Wednesday April 12, 2006. Please visit for details www.FocusOnMicroscopy.org

Focus on Microscopy 2006 is the continuation of a successful conference series presenting the latest innovations in optical microscopy and its applications in biology, medicine, material science, and information storage. 3D optical imaging and related theory are important subjects for the conference. The series is as relevant now as at any time in its history as the scientific and engineering communities strive to meet the needs of a surging life sciences sector as well as respond to the sustained pressure on miniaturisation in lithography and data storage.

Also a technical exhibition will be part of the conference. In Jena well over 30 companies participated including the major microscopy companies, see FOM2005 Sponsors & Exhibitors www.focusonmicroscopy.org/2005/sponsors.html .

The 2006 meeting will be held in Perth on Australia's western seaboard. The conference will be held in the nearby port city of Fremantle, at the scenic Esplanade hotel, close to the boat harbour and Perth' famous beaches. Perth's relaxed and outdoor lifestyle should prove an ideal setting for a stimulating and enjoyable meeting - see you there!

Local organising committee:
Stephen Cody
Central Resource for Advanced Microscopy, Ludwig Institute for Cancer Research, Melbourne

Guy Cox
Electron Microscope Unit, University of Sydney

Ewa Goldys
Division of Information and Communication Sciences, Macquarie University, Sydney

Brendan Griffin
Centre for Microscopy and Microanalysis, University of Western Australia, Perth

Miranda Grounds
School of Anatomy and Human Biology, University of Western Australia, Perth

Ian Harper
School of Biomedical Sciences, Monash University, Melbourne

David Jans
Department of Biochemistry and Molecular Biology, Monash University, Melbourne

Min Gu
Centre for Microphotonics, Swinburne University, Melbourne

John Kuo
Centre for Microscopy and Microanalysis, University of Western Australia, Perth

Keith Nugent
School of Physics, University of Melbourne

Paul Rigby
Biomedical Imaging and Analysis Facility, University of Western Australia, Perth

Alpha Yap
Institute for Biomolecular Science, University of Queensland, Brisbane

Conference topics include:
* Confocal and multiphoton-excitation microscopies * Novel illumination and detection strategies - selectiveplane, extended depth of focus, 4pi, structured illumination * Fluorescence - new labels, fluorescent proteins, quantum dots, single molecule, excitation-emission spectroscopy * Time-resolved fluorescence - FRET, FRAP, FLIM, FCS * Coherent non-linear microscopies - SHG, THG, SFG, CARS * Scattering processes: Raman, light scattering spectroscopy, second harmonic * Multi-dimensional imaging * Sub-wavelength resolution - near field microscopy, total internal reflection * Laser manipulation, ablation and microdissection, photoactivation * Magnetic resonance and X-ray microscopy * Image processing and visualisation * Live cell and tissue imaging * Whole tissue imaging - optical coherence tomography, endoscopy, whole animal fluorescence * New tools in genomics, proteomics, phenomics, cytometry * Lithography and data storage

To stay informed on the program, registration and abstract submission for the conference please leave your E-mail address here www.focusonmicroscopy.org/forms/subscr_notification.html .

On behalf of the FocusOnMicroscopy society,
* David Sampson, University of Western Australia
* Fred Brakenhoff, University of Amsterdam, The Netherlands

Stephen H. Cody

Microscopy Manager
Central Resource for Advanced Microscopy
Ludwig Institute For Cancer Research
PO Box 2008 Royal Melbourne Hospital
Parkville  Victoria    3050
Australia
Tel: 61 3 9341 3155    Fax: 61 3 9341 3104
email: stephen.cody-at-ludwig.edu.au
www.ludwig.edu.au/labs/confocal.html
www.ludwig.edu.au/confocal




------------------------------Original Headers------------------------------
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From: baskin-at-bio.umass.edu
Date: Tue, 5 Jul 2005 14:28:09 -0500
Subject: [Microscopy] postdoc open in computer vision for cell motility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Postdoctoral Position: Computer Vision Algorithms for Studying
Biological Growth and Motility

A postdoctoral position is available to join a research
project in the quantification of deformable motion in biology, with
emphasis on growth and cell motility. The position is supported by an
NIH-funded collaboration between Tobias I. Baskin (a biologist at
Umass Amherst) and K. Palaniappan (a computer scientist at University
of Missouri, Columbia). Baskin and Palaniappan have developed new
software for quantifying the spatial distribution of velocity within
a growing plant organ (a root). The software is called RootflowRT and
the biological application is described by van der Weele et al (2003
Plant Physiology, 32:1138-1148). The software implements a novel
algorithm for quantifying deformable motion that combines
structure-tensor and robust-matching approaches. The project is to
enhance and validate RootFlowRT, apply software engineering
principles to the current code base, explore new computational
algorithms, and extend the robust-tensor approach to other kinds of
biological objects, in particular motile animal cells and embryos.
The open position is at Amherst and involves imaging different kinds
of biological object as well as enhancing the software. Applicants
should have experience in some area of image processing, good
programming skills, and, preferably, experience in biology.

Those interested in the position should contact Dr Baskin
(email: baskin-at-bio.umass.edu), and can find further information from
his web page: http://www.bio.umass.edu/biology/baskin/ and the page
for RootflowRT http://meru.rnet.missouri.edu/mvl/bio_motion.

I encourage applications from anyone regardless of skin
color, religion, sex, sexual orientation, or nationality.

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
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From: zaluzec-at-microscopy.com
Date: Tue, 5 Jul 2005 18:37:28 -0500
Subject: [Microscopy] DiI for morphology measurements?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi, Is anyone aware of a method using DiI for staining cells growing in a
monolayer thickness, targeting the cell membrane, to obtain enough contrast to
yield a binary image of the cells representing their shape/morphology?

I'm growing multi-potent cells in 24-well plates and would like to quantify
their shape (and changes in shape) as they differentiate with time.

I've looked in the literature, and I've found lots of examples of DiI being used
to label neurons in live tissue. However, these papers don't focus so much on
quantitative morphological measurements, but more on what the neurons are
ennervating. I'd like a simple method for cells growing or fixed in a plate or
on a slide, for quantifying shape.

Any suggestions?

Sincerely,
Mike McIntyre

X-from: mamcin-at-umich.edu

==============================Original Headers==============================
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==============================Original Headers==============================
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From: marie.cantino-at-uconn.edu
Date: Wed, 6 Jul 2005 13:39:00 -0500
Subject: [Microscopy] EM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for companies or individuals providing repair services for
TEMs and SEMs in the New England area. Does anyone know of a
comprehensive list of contacts? Any recommendations?

Any providers or others with relevant information are welcome to
contact me at the phone number listed below, or by e-mail. Thanks.

Marie

Dr. Marie E. Cantino
Director, Electron Microscopy Laboratory
Associate Professor of Physiology and Neurobiology
University of Connecticut Unit 3242
Storrs, CT 06269-3242
Phone: 860-486-3588
Fax: 860-486-6369


==============================Original Headers==============================
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5, 20 -- Reply-To: Cantino Marie {marie.cantino-at-uconn.edu}
5, 20 -- From: Marie Cantino {marie.cantino-at-uconn.edu}
5, 20 -- Subject: EM service
5, 20 -- Date: Wed, 6 Jul 2005 14:44:15 -0400
5, 20 -- To: MSA Listserver {Microscopy-at-msa.microscopy.com}
5, 20 -- X-Mailer: Apple Mail (2.622)
5, 20 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information.
5, 20 -- X-UConn-MailScanner: Found to be clean
5, 20 -- X-UConn-MailScanner-SpamCheck:
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From: smalinskas-at-yahoo.com
Date: Wed, 6 Jul 2005 14:11:48 -0500
Subject: [Microscopy] Re: [Microscopy] EM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I can recommend the following:

Ken Converse
Quality Images
Delta PA
(717) 456-5491

He participates in this newsgroup.

Stu Smalinskas
Metallurgist
SKF
Plymouth, Michigan

--- marie.cantino-at-uconn.edu wrote:

}
}
}
----------------------------------------------------------------------------
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} Microscopy Society of America
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} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
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}
} I am looking for companies or individuals providing
} repair services for
} TEMs and SEMs in the New England area. Does anyone
} know of a
} comprehensive list of contacts? Any
} recommendations?
}
} Any providers or others with relevant information
} are welcome to
} contact me at the phone number listed below, or by
} e-mail. Thanks.
}
} Marie
}
} Dr. Marie E. Cantino
} Director, Electron Microscopy Laboratory
} Associate Professor of Physiology and Neurobiology
} University of Connecticut Unit 3242
} Storrs, CT 06269-3242
} Phone: 860-486-3588
} Fax: 860-486-6369
}




____________________________________________________
Sell on Yahoo! Auctions – no fees. Bid on great items.
http://auctions.yahoo.com/

==============================Original Headers==============================
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9, 19 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
9, 19 -- Subject: Re: [Microscopy] EM service
9, 19 -- To: marie.cantino-at-uconn.edu, microscopy-at-ns.microscopy.com
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From: GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 6 Jul 2005 14:20:59 -0500
Subject: [Microscopy] Uranyl Acetate Shelf Life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm interested in hearing how long people keep the Uranyl Acetate stain that
they use in grid staining, both the stock and working solution. Currently
we are using a 2% solution in 50% methanol.

How long do you think one could say that they remain fresh after making up?
Presently our lab keeps the stock solution for about a month, but I never
really had any literature to know one way or the other if I could keep it
long.


This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

==============================Original Headers==============================
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From: edelmare-at-muohio.edu
Date: Wed, 6 Jul 2005 16:05:09 -0500
Subject: [Microscopy] Re: [Microscopy] RE: High Res Monitors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Just some thoughts. . .

We've been using multiple LCD monitors lately to increase our
windows desktop size. However, in doing so I have noted that
Matrox does offer a high-resolution graphics card (3840x2400) {
http://www.matrox.com/mga/workstation/3dws/products/special/hr25
6.cfm } and they point to three monitors for this card something
refed to as 9 MP (assume 9 mega pixel ? maybe) monitors
(Viewsonic, IBM T221, and Iiayam)

NVidia Quadro FX series cards also goes to 3840x2400

ATI as a few cards which will deliver 3840 x 2400 (using multiple
monitors)

IBM lists a T221 as their 9.2 megapixel monitor - but I can not find
its avavilablity.

A company called Planar makes a 5-megapixel greyscale monitor.
Dome C5i

www.tridentmicrosystems.co.uk lists a 28.1” 2k x 2k TFT LCD
monitor is designed for traffic management.

NEC lists 2048 x 1536 as their highest resolution monitor. (But
does include 10-bit greyscale as well).

Viewsonic offers a number of "Thin Edge" monitors designed to
used together including stands designed to hold 2,3 or 4 monitors
at once.

There is a company www.9xmedia.com that offers multimonitor
solutions.



(Note: I do not sell montiors, or own stock / interests in any monitor
graphics company. :-)



} ------------------------------------------------------------------------------
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} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
} Don;
} I tried to purchase an IBM T-220 9 megapixel display, but was
} told by IBM that they had killed the product. This was the only display
} ever marketed that could display a 2K X 2K camera image showing all of
} the pixels on one screen. We still have two screens on our TEM, but need
} to either display the images at half resolution, or zoom to display only
} part of the image.
}
} John Mardinly
}
} -----Original Message-----
} } From: Don Chernoff at ASM [mailto:donc-at-asmicro.com]
} Sent: Friday, July 01, 2005 9:05 AM
} To: Microscopy List
} Subject: [Microscopy] Image review: Hard copy vs. PC screen
}
}
}
} ------------------------------------------------------------------------
} ------
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} http://www.microscopy.com/MicroscopyListserver
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} ------------------------------------------------------------------------
} -------
}
} This post is tangential to the dye sub vs ink jet discussion.
} When one needs to compare 2 or more images simultaneously, prints can
} easily
} be laid out on a table, but this is not so easy with on-screen display
} of
} digital images. One thing that we have done in our lab is to install a
} second monitor at some workstations and increase the Windows desktop
} size so
} that it spans the two monitors. This is satisfactory for AFM images
} where
} the basic pixel count is 512x512 but may not be so good with other
} formats.
} I am curious to learn what other people do.
}
} When you give your customers digital images only, do you feel there is a
} risk they could miss an important comparison?
}
} regards,
} Don Chernoff
} ==================================
} Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
} 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
} INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA &
} Canada)
} web: http://www.asmicro.com Fax: 317-895-5652
} [business activities: analytical services in AFM, AFM probes,
} consulting,
} training,
} calibration and test specimens, calibration and measurement software,
} used NanoScope equipment.]
}
}
}
}



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"WE ARE MICROSOFT.
RESISTANCE IS FUTILE.
YOU WILL BE ASSIMILATED."


==============================Original Headers==============================
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From: uti-at-direcpc.com
Date: Thu, 7 Jul 2005 06:41:13 -0500
Subject: [Microscopy] Ze E10 - entgen ete bad

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear group,

We have an old Zeiss EM10C, which we try to put back to the service.
It come with dual channel Roentgen meter board, MOSFET.
Unfortunately we do not have a circuit diagram, which will allow us to fix
a problem on the board.
Some one has a copy of diagram of that board?

Thanks,
Vlad



==============================Original Headers==============================
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From: jmjenks-at-pacbell.net
Date: Thu, 7 Jul 2005 10:09:30 -0500
Subject: [Microscopy] vaWWW: en EA (Eletn be Anal) entt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(jmjenks-at-pacbell.net) from http://microscopy.com/MLFormMail.html
on Thursday, July 7, 2005 at 09:31:04
---------------------------------------------------------------------------

Email: jmjenks-at-pacbell.net
Name: Jeff Jenks

Organization: micronetpartners.com

Title-Subject: [Filtered] Senior EPMA (Electron Probe Micro Analysis) Scientist position

Question: Senior EPMA (Electron Probe Micro Analysis) Scientist position:

Silicon Valley based semiconductor equipment company seeks Senior EPMA Scientist as follows:

Mission and Duties:

- Actively participate in the development, testing and evaluation of new metrology solutions for semiconductor processing from the concept stage to prototype testing.

- Provide a broad practical and theoretical knowledge base of electron spectroscopy, X-ray optics, and electron probe micro analysis (EPMA) to assess analytical capabilities, application areas and metrology opportunities of various analysis techniques.

- Work with a team of scientists and engineers in system development, prototype testing, and design of experiments.

- Plan, perform and evaluate hardware and application feasibility studies on test setups and complete prototype systems.



Responsibilities:

- Independently perform and evaluate experiments using prototype systems

- Design experiments for EPMA concept feasibility

- Build and modify experimental test systems using hands-on skills

- Provide and maintain up-to-date knowledge of surface characterization techniques

- Model and predict electron and X-ray intensities due to particle-material interaction

- Develop, test, and evaluate measurement protocols

- Actively participate in system development and subsystem designs



Background and Education:

- MS or Ph.D. in material science, physics or related field is required; an emphasis in measurement or instrumentation is preferred

- Broad knowledge in material analysis

- Strong practical experience in electron probe analysis, preferably WDX Wavelength- Dispersive X-ray Spectroscopy or EPMA or equivalent

- Experience in semiconductor material analysis is preferred but not required

- Intimate knowledge of data reduction methods, statistical analysis and principles of particle-material interaction

- Knowledge of X-ray optics and/or X-ray optical design is desirable

- Ability to work independently and within a team of scientists and engineers

- Minimum of 2 years of practical experience in a combination of the above areas



Company offers competitive salary, stock options, benefits, and relocation assistance in a collegial work environment. Company is privately held and VC-funded with outstanding prospects for stock price appreciation in the future.



For immediate consideration please contact Jeff Jenks with your resume or CV at Jeff-at-micronetpartners.com. Or you can call Jeff at 408-850-7271 for additional details. No visa sponsorship is available. For additional openings please view www.micronetpartners.com/jobs.html.


---------------------------------------------------------------------------

==============================Original Headers==============================
40, 13 -- From zaluzec-at-microscopy.com Thu Jul 7 10:09:30 2005
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40, 13 -- To: microscopy-at-microscopy.com
40, 13 -- From: jmjenks-at-pacbell.net (by way of MicroscopyListserver)
40, 13 -- Subject: viaWWW: Senior EPMA (Electron Probe Micro Analysis) Scientist
40, 13 -- position
40, 13 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Thu, 7 Jul 2005 10:45:32 -0500
Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Sorry, I seem to have scrambled the subject line of the last few postings
with my latest version of the filter code. Please do not critique the
person posting it was my fault.


Nestor
Your Friendly Neighborhood SysOp

--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
NEESLab: http://neestpm.mcs.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================

==============================Original Headers==============================
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7, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
7, 14 -- Subject: Administrivia: Subject Line scrambled...sorry
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==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Thu, 7 Jul 2005 10:48:26 -0500
Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Colleagues

Sorry, I seem to have scrambled the subject line of the last few postings
with my latest version of the filter code. Please do not critique the
person posting it was my fault.


Nestor
Your Friendly Neighborhood SysOp




--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
NEESLab: http://neestpm.mcs.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================

==============================Original Headers==============================
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13, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
13, 14 -- Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry
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From: jmjenks-at-pacbell.net
Date: Thu, 7 Jul 2005 10:48:44 -0500
Subject: [Microscopy] viaWWW: Senior EPMA (Electron Probe Micro Analysis) Scientist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(jmjenks-at-pacbell.net) from http://microscopy.com/MLFormMail.html
on Thursday, July 7, 2005 at 09:31:04
---------------------------------------------------------------------------

Email: jmjenks-at-pacbell.net
Name: Jeff Jenks

Organization: micronetpartners.com

Title-Subject: [Filtered] Senior EPMA (Electron Probe Micro Analysis) Scientist position

Question: Senior EPMA (Electron Probe Micro Analysis) Scientist position:

Silicon Valley based semiconductor equipment company seeks Senior EPMA Scientist as follows:

Mission and Duties:

- Actively participate in the development, testing and evaluation of new metrology solutions for semiconductor processing from the concept stage to prototype testing.

- Provide a broad practical and theoretical knowledge base of electron spectroscopy, X-ray optics, and electron probe micro analysis (EPMA) to assess analytical capabilities, application areas and metrology opportunities of various analysis techniques.

- Work with a team of scientists and engineers in system development, prototype testing, and design of experiments.

- Plan, perform and evaluate hardware and application feasibility studies on test setups and complete prototype systems.



Responsibilities:

- Independently perform and evaluate experiments using prototype systems

- Design experiments for EPMA concept feasibility

- Build and modify experimental test systems using hands-on skills

- Provide and maintain up-to-date knowledge of surface characterization techniques

- Model and predict electron and X-ray intensities due to particle-material interaction

- Develop, test, and evaluate measurement protocols

- Actively participate in system development and subsystem designs



Background and Education:

- MS or Ph.D. in material science, physics or related field is required; an emphasis in measurement or instrumentation is preferred

- Broad knowledge in material analysis

- Strong practical experience in electron probe analysis, preferably WDX Wavelength- Dispersive X-ray Spectroscopy or EPMA or equivalent

- Experience in semiconductor material analysis is preferred but not required

- Intimate knowledge of data reduction methods, statistical analysis and principles of particle-material interaction

- Knowledge of X-ray optics and/or X-ray optical design is desirable

- Ability to work independently and within a team of scientists and engineers

- Minimum of 2 years of practical experience in a combination of the above areas



Company offers competitive salary, stock options, benefits, and relocation assistance in a collegial work environment. Company is privately held and VC-funded with outstanding prospects for stock price appreciation in the future.



For immediate consideration please contact Jeff Jenks with your resume or CV at Jeff-at-micronetpartners.com. Or you can call Jeff at 408-850-7271 for additional details. No visa sponsorship is available. For additional openings please view www.micronetpartners.com/jobs.html.


---------------------------------------------------------------------------

==============================Original Headers==============================
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40, 13 -- To: microscopy-at-microscopy.com
40, 13 -- From: jmjenks-at-pacbell.net (by way of MicroscopyListserver)
40, 13 -- Subject: viaWWW: Senior EPMA (Electron Probe Micro Analysis) Scientist
40, 13 -- position
40, 13 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: zaluzec-at-aaem.amc.anl.gov
Date: Thu, 7 Jul 2005 10:51:16 -0500
Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

Sorry, I seem to have scrambled the subject line of the last few postings
with my latest version of the filter code. Please do not critique the
person posting it was my fault.

I have reposted at least one of the scrambled messages.


Nestor
Your Friendly Neighborhood SysOp



==============================Original Headers==============================
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8, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
8, 14 -- Subject: [Microscopy] Administrivia: Subject Line scrambled...sorry
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From: cpetty1-at-umbc.edu
Date: Thu, 7 Jul 2005 11:58:28 -0500
Subject: [Microscopy] TEM local service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings from Baltimore. I have a Zeiss 10 CA. Our past service company
Pesto Inc. longer is in business. Does anyone know of a person or
company that works on Zeiss TEMs in my area?
--
Chere Petty, M.S.
Manager, Keith R. Porter Imaging Facility
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
1000 Hilltop Circle
Baltimore, MD 21250
Phone: 410-455-2296
Fax: 410-455-3875

==============================Original Headers==============================
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1, 21 -- From: Chere Petty {cpetty1-at-umbc.edu}
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From: uti-at-direcpc.com
Date: Thu, 7 Jul 2005 12:06:12 -0500
Subject: [Microscopy] Zeiss EM10C - Roentgen meter board

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Dear group,

We have an old Zeiss EM10C, which we try to put back to the service.
It come with dual channel Roentgen meter board, MOSFET.
Unfortunately we do not have a circuit diagram, which will allow us to fix
a problem on the board.
Some one has a copy of diagram of that board?

Thanks,
Vlad




==============================Original Headers==============================
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9, 15 -- To: microscopy-at-microscopy.com
9, 15 -- From: uti-at-direcpc.com (by way of Nestor J. Zaluzec)
9, 15 -- Subject: [Microscopy] Zeiss EM10C - Roentgen meter board
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From: vincent.metzger-at-philips.com
Date: Thu, 7 Jul 2005 13:01:05 -0500
Subject: [Microscopy] viaWWW: Inca energy - real K-ratio ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(vincent.metzger-at-philips.com) from http://www.microscopy.com/MLFormMail.html
on Thursday, July 7, 2005 at 11:26:23
---------------------------------------------------------------------------

Email: vincent.metzger-at-philips.com
Name: Vincent Metzger

Organization: philips

Title-Subject: [Filtered] Inca energy - real K-ratio ?

Question: I'm looking for thickness measurement on a stratified sample by using HT variation on SEM and a software to modelize theoricaly the interactions in the stratified sample.
I need to extract real Kratio.
I made some tests by passing a standart of silicium (for instance), then my sample (containig a strat of Si) and making the ratio between the 2 intensity. This work quite well, but rather time consuming.
I standardized my silicium in the inca energy software, but the K-ratio displayed was totally wrong.
As an example the system uses Co for quant optimization, and when you analyse the Co, a 1.6 ratio is found:crazy.

I'm wondering how the soft works, very ergonomic but rather a black box.
What coefficient is applied? How to get rid of?
Is there a option that can be disabled or something else?



---------------------------------------------------------------------------

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9, 12 -- From: vincent.metzger-at-philips.com (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: Inca energy - real K-ratio ?
9, 12 -- Content-Type: text/plain; charset="us-ascii"
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From: yaseki-at-ucsd.edu
Date: Thu, 7 Jul 2005 19:31:36 -0500
Subject: [Microscopy] =?iso-8859-1?Q?AskAMicroscopist=8A?= help for LEO 438VP SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55).
It was submitted by (yaseki-at-ucsd.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Thursday, July 7, 2005 at 18:25:53
---------------------------------------------------------------------------

Email: yaseki-at-ucsd.edu
Name: Seki Yasuaki

Organization: University of Calfornia-San Diego

Education: Graduate College

Location: La Jolla, CA, USA

Question: Looking for vacuum schemtics, connection help for LEO 438VP SEM. The disassembled instrument has an Edwards Turbo backed by Edwards RV12 pump. The isolation block has an additional port (NW25 fitting) for connection to the specimen chamber. The microscope also has a solenoid operated PV25EK valve. I am looking for info on where this valve is to be located? a) between the block and turbo? or b) between the block and the specimen chamber? or c) elsewher? Any pictures would be of immense help as well.

---------------------------------------------------------------------------

==============================Original Headers==============================
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7, 12 -- From: yaseki-at-ucsd.edu (by way of Ask-A-Microscopist)
7, 12 -- Subject: =?iso-8859-1?Q?AskAMicroscopist=8A?= help for LEO 438VP SEM
7, 12 -- Content-Type: text/plain; charset="us-ascii"
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From: benada-at-biomed.cas.cz
Date: Fri, 8 Jul 2005 04:23:12 -0500
Subject: [Microscopy] Carbon evaporation troubles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good day all,

My apologies if this posting a repeater- I had problems with my e-mail in
the past week.

Two similar models of this monitor were available: IBM T221 and Viewsonic
VP2290B. Both (I was told) used IBM display panel 22" diagonal, 3840 x 2400
pixels, 204
dpi. Both discontinued. I was also told that IBM had plans of replacing T221
with a
new model as early as March 2005. I don't know whether they did yet.

We purchased several such monitors, IBM and Viewsonic, for TEM camera
systems. Both models are similar in performance, except Viewsonic is a bit
slower in full resolution mode, which makes no difference for static images.
It's hard for a PC to run 9.2 MP frames at over 20 FPS refresh rate anyway.
In fact, it is better to run 1600 x 1200 desktop most of the time and switch
to 3840 x 2400 for high resolution viewing. 1600 x 1200 keeps fonts and
icons size comfortable, and refresh rate is fast. The display in 3840 x 2400
mode is stunning- like looking through a window on a sunny day. You can take
an eye loupe to the screen and see further detail in the image.

If you will be able to find refurbished or second hand T221 - IBM will honor
original 3 year factory warranty as long as monitor is not physically
damaged. One of our IBM monitors was refurbished, with a screen defect. IBM
replaced the monitor. Consult with IBM regarding the warranty, before buying
used monitor. Have monitor serial number when calling IBM. These monitors
are still available, mostly on e-bay, and through some internet outlets.
Could be even new in box, but not from a regular source.

Is anybody at IBM reading this message? Please comment on a future
availability of equal or better display monitor.

See IBM paper on T221 in EM application at
http://www-1.ibm.com/industries/healthcare/doc/content/bin/ls_t221_enhancing_electron_1.pdf

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
X-from: "Mardinly, John" {john.mardinly-at-intel.com}
To: "Don Chernoff at ASM" {donc-at-asmicro.com} ; "Microscopy List"
{microscopy-at-microscopy.com}
Sent: Saturday, July 02, 2005 3:53 AM

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

Hi,

We have troubles with evaporation control unit BSV 202 of our BALZERS BAF
301 FE device. Does anybody have some experiences with it?

The current needed for carbon evaporation suddenly jumped so high that the
8 A fuse blown out happend. After replacing it, the current needed for
carbon evaporation stays too high and exceeds the permitted current of
safeguarding 8A fuse. There are no differences if we use evaporator 1
(transformer 1) or evaporator 2 (transformer 2).
For carbon evaporation we use sharpenned carbon rods.

Thanking in advance for any suggestion.
Oldrich
-------------------------------------------------------------------------
Oldrich Benada
Institute of Microbiology, Acad. Sci. CR
Lab. EM
Videnska 1083
142 20 Prague 4
Czech Republic


==============================Original Headers==============================
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From: mozaluzec-at-microscopy.com
Date: Fri, 8 Jul 2005 07:49:42 -0500
Subject: [Microscopy] Solid Mortgages for Americans

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Hello

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From: zaluzec-at-microscopy.com
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Subject: [Microscopy] Admin Test Message 9:32 AM

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Admin Test Message 9:32 AM
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From: edelmare-at-muohio.edu
Date: Fri, 8 Jul 2005 09:52:35 -0500
Subject: [Microscopy] Imaging thin films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

O.k., I'm a materials neophyte and I'm looking for some
direction. I can ultramicrotome things, I can crush and fracture
things, and I can physically polish things, but now I am facing a new
sample area: How do I prepare thin films, inorganic, crystal and
semi-crystaline grown on a hard smooth substraight (glass, Si,
Mica, etc.)? Film thickness below 1-micrometer, mostly below
300nm. We would like to study the morphology of the film via SEM
or TEM. EDS and EBSD would also be nice. So how do I get a
clean cross-section of the thin film? Buying a dual-column/beam
FIB/SEM might be a nice idea but seems a little over-kill, eh? Can
someone point me in the right direction? Ion-beam milling? Ion-
beam polishing? Sectioning glass or Si crystal just does not sound
like a good idea.

Next are there any thoughts on buying equipment and doing this in
house vs having samples preped off-campus (I acknowledge that
there are some techniques and equipment that are just really tricky).

Thank you!



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

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From: Mike.Bode-at-soft-imaging.net
Date: Fri, 8 Jul 2005 10:08:39 -0500
Subject: [Microscopy] For Nestor: Double posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

Hi Nestor,

As of today I am getting all postings twice. Is that a problem with my
subscription or a general problem?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================



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From: gary-at-gaugler.com
Date: Fri, 8 Jul 2005 10:24:45 -0500
Subject: [Microscopy] Re: Imaging thin films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

SEM will produce morphology info whereas EBSD will produce
grain, orientation and texture info. Totally different
results and prep methods.

Depending on what the film material is, that would dictate
how it is prepared for analysis. Silicon is easiest I think
to use as a substrate since it can easily be cut into small
pieces. Mount the piece and coat it then SEM image for
morphology. Mount another piece and polish it with colloidal
silica or alumina (again, depending on film material) down
to .02u and this should work for EBSD.

FIB ablades the surface and does not produce good EBSD specimens.
I've tried various plasmas and Gatan 682 and do not get good specimens
for EBSD. There is probably some magic combination that I have
yet to find. Thus far, mechanical polishing and slight etch
with DI water seems to do the job. There are dedicated ion beam
polishing tools that do nice jobs for EBSD. But unless you are
going to do a lot of this, I don't think it pencils out.

Since the analysis is tops down, I don't see why you would
need to section the specimen. If there is more to your
situation, tell us more.

gary g.


At 07:55 AM 7/8/2005, you wrote:

} O.k., I'm a materials neophyte and I'm looking for some
} direction. I can ultramicrotome things, I can crush and fracture
} things, and I can physically polish things, but now I am facing a new
} sample area: How do I prepare thin films, inorganic, crystal and
} semi-crystaline grown on a hard smooth substraight (glass, Si,
} Mica, etc.)? Film thickness below 1-micrometer, mostly below
} 300nm. We would like to study the morphology of the film via SEM
} or TEM. EDS and EBSD would also be nice. So how do I get a
} clean cross-section of the thin film? Buying a dual-column/beam
} FIB/SEM might be a nice idea but seems a little over-kill, eh? Can
} someone point me in the right direction? Ion-beam milling? Ion-
} beam polishing? Sectioning glass or Si crystal just does not sound
} like a good idea.
}
} Next are there any thoughts on buying equipment and doing this in
} house vs having samples preped off-campus (I acknowledge that
} there are some techniques and equipment that are just really tricky).
}
} Thank you!
}
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 350 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu


==============================Original Headers==============================
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9, 19 -- Subject: Re: [Microscopy] Imaging thin films
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From: biology-at-ucla.edu
Date: Fri, 8 Jul 2005 10:49:40 -0500
Subject: [Microscopy] via-WWW: Looking for Judy Murphy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (biology-at-ucla.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, July 8, 2005 at 10:41:07
---------------------------------------------------------------------------

Email: biology-at-ucla.edu
Name: Eric A. Rosen

Organization: UCLA Medical Center

Title-Subject: I am trying to get a hold of Judy Murphy at the San Joaquin Delta

Question: I am trying to get a hold of Judy Murphy at the San Joaquin Delta College EM School
I have a open EM position and would like to advertise it with her...


Thanks

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: phillipst-at-missouri.edu
Date: Fri, 8 Jul 2005 13:17:07 -0500
Subject: [Microscopy] paraffin microtomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello John,

I am not quite sure what it means when you say that the resolution of the
human eye is 300 DPI. I looked up an article on the web
(http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which
specifies the angular resolution of the human eye under optimum conditions
as 1/60th of a degree. If I do my math right, the resolution is then given
by:

2 x distance x tan (angle/2).

For a viewing distance of about 1m, I get a resolution of 0.3mm (which
corresponds to about 220 DPI at 1m distance), which is more or less the dot
pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of
0.294mm). So, I think the normal monitors are actually at the limits of the
human eye's resolution. If your eyes were much better, you should be able to
see the individual red, green, and blue dots from a distance of 1m.

As I said, the optimum resolution assumes certain parameters: 25 cm viewing
distance, perfect lighting, etc. All changes will reduce the resolution.

But that's all mathematics. Has anybody actually compared a high res monitor
and a regular monitor side by side?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 10:04 AM
To: Mike Bode

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

I am considering buying a paraffin microtome for our core facility. I don't
personally use one much so if any knowledgeable users have recommendations
on brands or models to buy (or avoid), i would welcome replies (probably
best sent to me directly and not the listserver). I will be happy to keep
your comments confidential. In addition, if there are special features you
think are essential, i would be pleased to hear of them also. thanks, tom



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: mcauliff-at-umdnj.edu
Date: Fri, 8 Jul 2005 16:04:46 -0500
Subject: [Microscopy] Re: ink jet versus dye sub versus Pictrography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike;
One meter viewing distance? Sorry, my arms are no where near that long. Fortunately my presbyopia is compensated by severe myopia. Otherwise, use reading glasses. Closer up (~1 foot?), the human eye can resolve ~80-100 microns, which is 250-300 DPI, which is why probably why 300 DPI has been a target spec for printers for a long time. The IBM T221 has a pixel pitch of 0.1245 millimeters, so those pixels are half the size of the pixels of garden variety monitors that cost less, and the way that translates into 200DPI is (25 mm/in)/(0.1245 mm/pixel)=200.8 pixels/inch. BTW, I can see the vertical mask stripes of my Sony monitor with my naked eye if I get close, and everybody in our lab could see the difference between a 400DPI Fuji Pictrograph print and a monitor display.

John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Mike.Bode-at-soft-imaging.net [mailto:Mike.Bode-at-soft-imaging.net]
Sent: Friday, July 08, 2005 9:46 AM
To: Mardinly, John

Hello John,

I am not quite sure what it means when you say that the resolution of the
human eye is 300 DPI. I looked up an article on the web
(http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which
specifies the angular resolution of the human eye under optimum conditions
as 1/60th of a degree. If I do my math right, the resolution is then given
by:

2 x distance x tan (angle/2).

For a viewing distance of about 1m, I get a resolution of 0.3mm (which
corresponds to about 220 DPI at 1m distance), which is more or less the dot
pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of
0.294mm). So, I think the normal monitors are actually at the limits of the
human eye's resolution. If your eyes were much better, you should be able to
see the individual red, green, and blue dots from a distance of 1m.

As I said, the optimum resolution assumes certain parameters: 25 cm viewing
distance, perfect lighting, etc. All changes will reduce the resolution.

But that's all mathematics. Has anybody actually compared a high res monitor
and a regular monitor side by side?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 10:04 AM
To: Mike Bode

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

Hi John,

I was actually talking about monitors. I agree with you that you normally
look at prints at a shorter distance, but I think 1 m for a monitor is a
reasonable distance.

And what you are saying ("BTW, I can see the vertical mask stripes of my
Sony monitor with my naked eye if I get close") basically confirms my
assumption: If you are NOT up close, you can't see the stripe, so the pixel
size is close to resolution of the eye (at normal viewing distances). You
would not be able to see or detect a further reduction in pixel size. I
don't know if the width of the vertical mask is the same as a pixel. I, for
one, cannot see individual green, red and blue pixels when I look at a white
spot on the monitor, unless I use a magnifying glass.

Comparing printers and monitors is difficult. One is "back-illuminated", the
other shows reflected light, the color gamut is different, etc. I'd be
interested to hear from someone who has compared a normal LCD monitor with a
high resolution LCD monitor.

mike



Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:11 PM
To: Mike Bode
Cc: Listserver

Hello John,

I am not quite sure what it means when you say that the resolution of the
human eye is 300 DPI. I looked up an article on the web
(http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which
specifies the angular resolution of the human eye under optimum conditions
as 1/60th of a degree. If I do my math right, the resolution is then given
by:

2 x distance x tan (angle/2).

For a viewing distance of about 1m, I get a resolution of 0.3mm (which
corresponds to about 220 DPI at 1m distance), which is more or less the dot
pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of
0.294mm). So, I think the normal monitors are actually at the limits of the
human eye's resolution. If your eyes were much better, you should be able to
see the individual red, green, and blue dots from a distance of 1m.

As I said, the optimum resolution assumes certain parameters: 25 cm viewing
distance, perfect lighting, etc. All changes will reduce the resolution.

But that's all mathematics. Has anybody actually compared a high res monitor
and a regular monitor side by side?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 10:04 AM
To: Mike Bode

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

Mike;
Phil Batson reported at M&M 2004 that it was extremely useful and highly recommended. Vitaly Feingold reported this week in the Listserver that he has two. Perhaps he can also describe the experience of beholding an IBM T221.

John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Mike.Bode-at-soft-imaging.net [mailto:Mike.Bode-at-soft-imaging.net]
Sent: Friday, July 08, 2005 12:49 PM
To: Mardinly, John

Hi John,

I was actually talking about monitors. I agree with you that you normally
look at prints at a shorter distance, but I think 1 m for a monitor is a
reasonable distance.

And what you are saying ("BTW, I can see the vertical mask stripes of my
Sony monitor with my naked eye if I get close") basically confirms my
assumption: If you are NOT up close, you can't see the stripe, so the pixel
size is close to resolution of the eye (at normal viewing distances). You
would not be able to see or detect a further reduction in pixel size. I
don't know if the width of the vertical mask is the same as a pixel. I, for
one, cannot see individual green, red and blue pixels when I look at a white
spot on the monitor, unless I use a magnifying glass.

Comparing printers and monitors is difficult. One is "back-illuminated", the
other shows reflected light, the color gamut is different, etc. I'd be
interested to hear from someone who has compared a normal LCD monitor with a
high resolution LCD monitor.

mike



Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:11 PM
To: Mike Bode
Cc: Listserver

Hello John,

I am not quite sure what it means when you say that the resolution of the
human eye is 300 DPI. I looked up an article on the web
(http://www.madsci.org/posts/archives/may97/864446241.Ph.r.html), which
specifies the angular resolution of the human eye under optimum conditions
as 1/60th of a degree. If I do my math right, the resolution is then given
by:

2 x distance x tan (angle/2).

For a viewing distance of about 1m, I get a resolution of 0.3mm (which
corresponds to about 220 DPI at 1m distance), which is more or less the dot
pitch of a normal monitor (a 21" LCD at 1600x1200 has a dot pitch of
0.294mm). So, I think the normal monitors are actually at the limits of the
human eye's resolution. If your eyes were much better, you should be able to
see the individual red, green, and blue dots from a distance of 1m.

As I said, the optimum resolution assumes certain parameters: 25 cm viewing
distance, perfect lighting, etc. All changes will reduce the resolution.

But that's all mathematics. Has anybody actually compared a high res monitor
and a regular monitor side by side?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Mardinly, John [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 10:04 AM
To: Mike Bode

Hi all,

I have been following this thread with interest, as it affects us of course.
We would like to use higher resolution monitors for our TEM cameras, but
perhaps you could answer two questions for me:

1) What exactly would be the advantage of a high resolution monitor? Unless
you can see the pixilation on a regular monitor, a higher resolution monitor
would not show much more. To see more, the screen would also have to be much
bigger. Of course, you take a magnifying glass to the monitor and see more,
but that you can do with a zoom function on a regular monitor as well.

2) Is this worth around $10K or more to you?

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 1:29 AM
To: Mike Bode

Vitally;
Lucky you for getting these monitors. At $8400 for the monitor
and $2500 for the video card, not too many of us can get the funds for
one, but hey, compared to the price a new TEM, why don't new TEMs come
standard with at least TWO of these? I have yet to even see one. IBM
told me that the T220 was the original 9 megapixel monitor. It was
discontinued and then revived as the T221 with a 48 hertz refresh rate
and a font handling utility so that fonts could be displayed in a
readable size when the monitor was in 3840x2400 mode. What I don't
understand, is that over a month after they refused to sell one to me
because it was discontinued, it is still listed for sale on the IBM web
site!
http://www-1.ibm.com/servers/intellistation/pro/t221/index.html


John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Vitaly Feingold [mailto:vitalylazar-at-att.net]
Sent: Thursday, July 07, 2005 11:40 PM
To: Microscopy Listserver; Don Chernoff at ASM; Mardinly, John

John et al.:

A few more details on what I wrote on this subject before. Lyson has
been making inks and papers for photo quality ink jet printing for some
time. Their new system is the "Daylight Darkroom". It is a dedicated 4
to 7 ink system (depending on your printer) for black and white printing
only. I think the price includes software and inks but no printer, the
system only works with certain printers. It has gotten very favorable
reviews in fine art photo magazines (May/June 2005 issue of Photo
Techniques, the review might be on the magazine's website
(www.phototechmag.com) or on Lyson's). Lyson will send you some sample
prints if you register at their website. I suspect that if someone sent
Lyson a digital TEM or SEM image they might make a print of that as a
sales tool. I'll bet they have no idea that there is a market for their
products in the EM field. I only wish I did more than a few prints per
year of parts of ground up cells.
Lyson is not the only firm making inks, papers and printing systems
for fine art B&W photography. Check out InkjetMall.com or MediaStreet.com.
Disclaimer: I don't have any financial interest in any of the firms
or products I mentioned.

Geoff


John J. Bozzola wrote:

} ------------------------------------------------------------------------------
}
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -------------------------------------------------------------------------------
}
}
} We are comparing dye sub printers versus the Fuji Pictrography PG4500.
} The price differential is a staggering $22,000 versus $6,500,
} respectively. It is my understanding that both produce good quality,
} gray-scale prints (versus dithered, inkjet prints). Why the price
} differential? I would welcome any comments, user experiences, etc.
} Does anyone know the average cost per 8.5 x 11in print?
}
} Thank you.

--

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: cgarber-at-2spi.com
Date: Fri, 8 Jul 2005 17:02:15 -0500
Subject: [Microscopy] Characterization of thin film coatings by TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard E. Edelmann wrote:
===========================================
O.k., I'm a materials neophyte and I'm looking for some
direction. I can ultramicrotome things, I can crush and fracture
things, and I can physically polish things, but now I am facing a new
sample area: How do I prepare thin films, inorganic, crystal and
semi-crystaline grown on a hard smooth substraight (glass, Si,
Mica, etc.)? Film thickness below 1-micrometer, mostly below
300nm. We would like to study the morphology of the film via SEM
or TEM. EDS and EBSD would also be nice. So how do I get a
clean cross-section of the thin film? Buying a dual-column/beam
FIB/SEM might be a nice idea but seems a little over-kill, eh? Can
someone point me in the right direction? Ion-beam milling? Ion-
beam polishing? Sectioning glass or Si crystal just does not sound
like a good idea.

Next are there any thoughts on buying equipment and doing this in
house vs having samples preped off-campus (I acknowledge that
there are some techniques and equipment that are just really tricky).
===============================================
Since you are already set up to do ultramicrotomy, have you considered the following:

a) Take a freshly cleaved stripping of HOPG and then do your evaporation. If you are
not familiar with HOPG, see URL
http://www.2spi.com/catalog/new/hopgsub.shtml In terms of smoothness, HOPG can be
atomically smooth, the size of the atomically smooth areas depending on the grade of
the HOPG selected.

b) Put the HOPG stripping in a flat embedding mold and then expose the coated
sample, HOPG side up, to an oxygen plasma, such as in one of our Plasma Prep II
plasma etchers, which will etch away the HOPG but leave your coating intact.

c) Fill the cavity containing the thin film, now with HOPG removed, with your
favorite Epon 812 substitute, or SPI-Pon 812 and after curing,

d) Face off the block and diamond knife thin section the coating.

A variation on this theme would be to substitute a freshly cleaved surface of NaCl
for the substrate, and after coating, the NaCl is dissolved away and the coating
when dry, can be embedded as above.

You should have no difficulty getting good TEM images of the coating.

We actually prefer diamond knife thin sectioning to other methods for this kind of
sample. It is not a method free of artifacts. But when they are ones that are
induced by the knife edge itself, they are directional and easily recognized as
being artifacts, but for the other approaches one might consider, the artifacts are
not directional and therefore much more difficult to recognize.

Disclaimer: SPI Supplies offers HOPG, plasma etchers, diamond knives, and NaCl
crystals so we would have a vested interest in promoting this approach relative to
others.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================


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From: cgarber-at-2spi.com
Date: Sat, 9 Jul 2005 03:09:02 -0500
Subject: [Microscopy] carbon evaporation problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Oldrich Benada wrote:
=================================================================
We have troubles with evaporation control unit BSV 202 of our BALZERS BAF
301 FE device. Does anybody have some experiences with it?

The current needed for carbon evaporation suddenly jumped so high that the
8 A fuse blown out happend. After replacing it, the current needed for
carbon evaporation stays too high and exceeds the permitted current of
safeguarding 8A fuse. There are no differences if we use evaporator 1
(transformer 1) or evaporator 2 (transformer 2).
For carbon evaporation we use sharpenned carbon rods.

Thanking in advance for any suggestion.
=================================================================
Have you switched sources for your "carbon" rods lately? First, some
vendors offering "carbon" rods are really offering graphite rods. It is my
own perception that most people who think they are using carbon rods are
actually using graphite rods. Also, even among graphite rods, there is a
large variation of resistivities (due to variations in density). So a
common problem is that one procures the wrong kind of rods (which would have
a resistivity that is out of range for their instrumental set up) so that in
order to get evaporation, they exceed the instrumental parameters of their
system. See URL
http://2spi.com/catalog/spec_prep/carbon-graphite-rods.html
for our simple "test" to judge which type of rod you are actually using.

If you have not switched rod vendors, then the second most common reason is
that you are not making a sharp enough "point". Hence the tip, in order to
get hot enough to evaporate carbon, draws so much current, you exceed the
limits of your system. For the optimum dimensions for rod tips, see the
drawings on URL
http://2spi.com/catalog/spec_prep/carbon-rods.shtml

Rods can be purchased pre-sharpened from all of the major suppliers of
consumables for EM laboratories, such as SPI, but if you want to make your
own presharpened points, carbon rod sharpeners can be purchased as well such
as are found on URL
http://2spi.com/catalog/spec_prep/carbon-rod-sharpener.shtml

Disclaimer: SPI Supplies offers carbon and graphite rods as well as
sharpeners.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
==================================================



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From: Mike.Bode-at-soft-imaging.net
Date: Sat, 9 Jul 2005 11:48:17 -0500
Subject: Re: [Microscopy] RE: RE: RE: RE: RE: Image review: Hard copy vs. PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It is unquestionably right that the distance of best resolution for the
human eye is about 25 cm. However, I was talking about working on a monitor,
and I am typically sitting at arm's length from it. I just measured my arm,
and it is 75 cm. I do not work with a monitor with my eyes 25 cm from the
screen. Would definitely give me headaches.

I am not trying to prove to everyone that high res monitors are useless, far
from it. But I am personally not convinced that it would be worth $10,000.
Considering the eye's resolution and the options of today's software, I
think you can easily get what you want on a lower res monitor.

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Friday, July 08, 2005 18:50
To: Mike Bode

Barbara;
YES! 10 inches is the right number! That's just slightly less than
the length of my arms, and slightly more than the length of my nose, so
everything I look at ends up at about 10 inches.

John Mardinly
Intel


The opinions of this author do not necessarily represent the opinions on
Intel Corporation.

-----Original Message-----
X-from: Barbara Foster [mailto:bfoster-at-mme1.com]
Sent: Friday, July 08, 2005 2:59 PM
To: Mardinly, John




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From: zaluzec-at-microscopy.com
Date: Sat, 9 Jul 2005 12:04:43 -0500
Subject: [Microscopy] Distance to the Monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike etal

For someone that also has far too much monitor/eye neck strain
I also work at 75 cm distance. To make matters worse my
desktop systems are both 3200x1200 pixels... (Dual HR Monitors)
and I sometimes run with multiple virtual desktops
(giving me an effective desktop area of 6400x3200 pixels. You can't
display this all at once, but switching is very quick (on a Mac or Linux box)

I guess this is just a comment on technology. We are now overloaded
sometimes with far too much information or we are just "parallell processing"
alot now adays. Thinking back I don't see how I used to get along with the
old 640x480 monitors or even worse the old VT100 text terminals.

Sigh....

Nestor
Your Friendly Neighborhood SysOp

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From: normzoo-at-yahoo.com
Date: Sun, 10 Jul 2005 10:24:37 -0500
Subject: [Microscopy] viaWWW: LM Nikon AFM microscope camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (normzoo-at-yahoo.com) from http://microscopy.com/MLFormMail.html on Sunday, July 10, 2005 at 04:46:26
---------------------------------------------------------------------------

Email: normzoo-at-yahoo.com
Name: Norman Shedlo

Title-Subject: [Filtered] MListserver: LM Nikon AFM microscope camera

Question: This piece of equipment seems to be relatively common. What is the usual reason for the Nikon AFM microflex shutter to stop working on this model of microscope camera?

The controller works fine and has fresh batteries.

Is this something that can be easily repaired?

If not, who can repair it.

Thank you for any help.

Norman

---------------------------------------------------------------------------

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From: walck-at-southbaytech.com
Date: Mon, 11 Jul 2005 01:18:19 -0500
Subject: [Microscopy] re: Imaging thin films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard,

If you can crush, fracture, and polish samples, you are well on your way to
preparing samples by other means. South Bay Technology, as well as our
competitors, sell complete lines of sample preparation equipment for doing
what you want to do. A lot of the expertise of sample prep has been built
into the instruments or is contained in the operating manuals. At the South
Bay Technology web site, www.southbaytech.com, you can find a list of
application notes and technical papers associated with each instrument. At
our competitors' sites, you can find similar information. South Bay
Technology isn't the only company that has an experienced microscopist on
staff (just the best, LOL -Sorry Wolfgang and Rocco. I just couldn't
resist.). There are also short courses that involve sample preparation.
Ron Anderson and I are teaching a one day short course on TEM sample
preparation at the M&M 2005 meeting that you might be interested in
attending. We, as well as our competitors, periodically announce short
courses on general EM preparation techniques and on specific techniques.

For your immediate needs, I would recommend the MicroCleave(TM) technique,
(aka Small Angle Cleavage Technique). This is our model 520 kit and there
is a "how to" instruction guide that I wrote that you can get at our site to
learn how to do this. It is applicable to silicon, III-V and II-V
compounds, SiC, sapphire, GaN, glass, and other brittle materials. It
produces extremely good samples and is fairly easy to learn how to do and to
teach students and doesn't require any more skills that you have already
stated that you have.

For using other sample prep techniques such as dimpling, ion milling, and
Tripod Polishing, you can also find specific information about these
techniques at our website or you can refer to the four MRS proceedings on
TEM Preparation for the Physical Sciences. The common editor in these four
proceedings is Ron Anderson. I am not in my office at the moment, or I
would give you the volume numbers, but I do remember three: 115, 254, and
480.

Another good way of finding out about these techniques is to find a
university with an electron microscopy center that has an experienced lab
director and ask for their advice. A few universities that immediately come
to mind since I visited or used their facilities are Carnegie Mellon Univ,
North Carolina State Univ, Ohio State Univ, Univ of Florida, Univ of
California at Irvine, and Penn State Univ. I hope that I haven't slighted
anyone. The people at these centers have dealt with a multitude of
materials and sample preparation techniques and have a wealth of
information. If they can't help you, they will know who can.

I hope this helps.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: +1-949-492-2600
FAX: +1-949-492-1499
walck-at-southbaytech.com

Disclaimer: South Bay Technology manufactures and sells instrumentation for
the preparation of EM samples.








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From: walck-at-southbaytech.com
Date: Mon, 11 Jul 2005 02:15:11 -0500
Subject: [Microscopy] re: carbon evaporation problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am not familiar with this unit. However, your description sounds like there is a short in the electrodes. Have you checked to see whether evaporated material has put a conductive path between your electrodes? Try putting a current through without the carbon rod to see if you still get a high or even a low current draw. If you do, clean the system.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: +1-949-492-2600
FAX: +1-949-492-1499
walck-at-southbaytech.com

-------- Original Message --------
} From: cgarber-at-2spi.com
} Sent: Saturday, July 09, 2005 4:14 AM
} To: Walck-at-SouthBayTech.com
} Subject: [Microscopy] carbon evaporation problem
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Oldrich Benada wrote:
} =================================================================
} We have troubles with evaporation control unit BSV 202 of our BALZERS BAF
} 301 FE device. Does anybody have some experiences with it?
}
} The current needed for carbon evaporation suddenly jumped so high that the
} 8 A fuse blown out happend. After replacing it, the current needed for
} carbon evaporation stays too high and exceeds the permitted current of
} safeguarding 8A fuse. There are no differences if we use evaporator 1
} (transformer 1) or evaporator 2 (transformer 2).
} For carbon evaporation we use sharpenned carbon rods.
}
} Thanking in advance for any suggestion.





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From: ekman-at-itg.uiuc.edu
Date: Mon, 11 Jul 2005 10:17:15 -0500
Subject: [Microscopy] LM- Light Microscopy Position - Beckman Institute at the University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Applicants are being sought for the position of Specialist Light
Microscopist {jobs/SeniorMicro.htm} in the Imaging Technology Group
(ITG) at the Beckman Institute at the University of Illinois at
Urbana-Champaign.

Information regarding this position can be found here:
http://www.itg.uiuc.edu/jobs/SeniorMicro.htm

More information on what we do at the Imaging Technology Group can be
found here:
http://www.itg.uiuc.edu/

Sincerely,

Jonathan M. Ekman
Beckman Institute for Advanced Science and Technology, Imaging
Technology Group
University of Illinois at Urbana-Champaign
405 N. Mathews Avenue
Urbana, IL 61801 USA
Tel: 217-244-6292
Fax: 217-244-6219


==============================Original Headers==============================
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6, 18 -- Subject: LM- Light Microscopy Position - Beckman Institute at the University
6, 18 -- of Illinois at Urbana-Champaign
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From: snydert-at-mcmaster.ca
Date: Mon, 11 Jul 2005 12:56:16 -0500
Subject: [Microscopy] viaWWW: JEOL-10S SAM Documentation

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
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Monday, July 11, 2005 at 12:42:58
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Email: snydert-at-mcmaster.ca
Name: Tom Snyder

Organization: McMaster University

Title-Subject: [Filtered] MListserver:JEOL-10S SAM Documentation

Question: Hello,

We are trying to get this Auger microscope up and running but are missing documentation about electron-optics and how they should be set. Specifically we are having trouble locating the beam.

If anyone has access to the documentation or knows what the settings should be I would appreciate any feedback. Also if you know of a good test to verify the opticas are working, that would be great info as well.

Thanks
Tom S

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From: svanhorn-at-notes.cc.sunysb.edu
Date: Mon, 11 Jul 2005 19:07:50 -0500
Subject: [Microscopy] AskAMicroscopist: DAP Weldwood contact cement

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Email: svanhorn-at-notes.cc.sunysb.edu
Name: Sue Van Horn

Organization: SUNY-at-StonyBrook

Education: Graduate College

Location: Stony Brook,NY,USA

Question: has anyone ever used DAP Weldwood contact cement mixed with xylene to help in picking up serial sections???..if so how did you use it and at what dilution???
thanks
sue

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From: xburany-at-yahoo.com
Date: Tue, 12 Jul 2005 07:44:15 -0500
Subject: [Microscopy] Used LM

Contents Retrieved from Microscopy Listserver Archives
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Good Morning.

We want purchase a used optical microscope with
digital camera. Please contact with me directly if you
can help. Thanks,

Meng Burany
mburany-at-uwindsor.ca
519 253-3000 ext.2605



____________________________________________________
Sell on Yahoo! Auctions – no fees. Bid on great items.
http://auctions.yahoo.com/

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From: lcgould-at-med.cornell.edu
Date: Tue, 12 Jul 2005 08:38:21 -0500
Subject: [Microscopy] Re: AskAMicroscopist: DAP Weldwood contact cement

Contents Retrieved from Microscopy Listserver Archives
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Sue,
I don't remember if it was contact cement or some other glue, but I do remember reading (years ago) about painting the top and bottom sides of the block with a dilute "stick-um" so that as the sections were cut, they adhered to one another via the layer of sticky stuff on the edges. I never tried it, and I don't know how, if the sections adhere so well, one is supposed to separate the ribbon into pieces that will fit on a grid.
I know this isn't much help, but at least you know you're not the only one who has heard of such an approach.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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From: zaluzec-at-microscopy.com
Date: Tue, 12 Jul 2005 09:08:07 -0500
Subject: [Microscopy] Administrivia: Nestor is testing a minor change : 9:07 AM

Contents Retrieved from Microscopy Listserver Archives
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Administrivia: Nestor is testing a minor change : 9:07 AM

ML Version 7

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From: zaluzec-at-microscopy.com
Date: Tue, 12 Jul 2005 09:10:38 -0500
Subject: [Microscopy] Test @ 9:10

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Is the reply to fixed?

9:10

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From: milleri-at-ohio.edu
Date: Tue, 12 Jul 2005 09:18:03 -0500
Subject: [Microscopy] Weldwood cement & serial sections

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Sue,
The use of Weldwood cement "diluted with one or two parts of commercially
available contact cement thinner" (20% n-butyl acetate, 80% mineral spirits)
to coat the leading and trailing block faces plus many other technique tips
for serial sectioning was published by WH Fahrenbach J. Electron Microscopy
Technique 1:387-398; 1984. See also EC Henry Stain Technol. 52: 59-60; 1977
who I think was first to use contact cement for this purpose


Iain Miller
Department of Biological Sciences
Ohio University
Athens, OH 45701

740-593-2120

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From: chrisjohnrhodes-at-hotmail.com
Date: Tue, 12 Jul 2005 11:07:35 -0500
Subject: [Microscopy] viaWWW: mounting medium

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (chrisjohnrhodes-at-hotmail.com) from on Tuesday, July 12, 2005 at 10:56:47
---------------------------------------------------------------------------

Email: chrisjohnrhodes-at-hotmail.com
Name: Chris Rhodes

Organization: Syracuse University

Title-Subject: [Filtered] MListserver:

Question: I'm looking for a semi-permanent mounting medium with the best possible refractive index match to immersion oil and a cover slip (ie as close as possible to 1.515).

The best I have found so far is 1.539

It is for mounting small (less than 2mm) insect structures.

It will be used for CLSM and thus would hopefully have minimal fluorescence.

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From: dsherman-at-purdue.edu
Date: Tue, 12 Jul 2005 12:00:35 -0500
Subject: [Microscopy] Film cassettes

Contents Retrieved from Microscopy Listserver Archives
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Folks,

Due to numerous camera jams over the years we are a bit short on film
cassettes for the Philips CM-10 and CM-100. I would appreciate your
contacting me if any of you have extra cassettes that you are willing to
give/sell to me.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



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From: mgoheen-at-iupui.edu
Date: Tue, 12 Jul 2005 13:37:06 -0500
Subject: [Microscopy] Film cassettes

Contents Retrieved from Microscopy Listserver Archives
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Hi Debby,

I got quite a few from our Philips 300 before we got rid of it. How many
do you need? I use them to replace bad ones in our CM10 and they work
fine.

Mike

Michael P. Goheen
Electron Microscopy Lab
Dept. of Pathology & Lab Medicine
Indiana University School of Medicine
Tel. 317-274-7604
Fax 317-274-5346
mgoheen-at-iupui.edu


-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: Tuesday, July 12, 2005 12:04 PM
To: Goheen, Michael P.

Folks,

Due to numerous camera jams over the years we are a bit short on film
cassettes for the Philips CM-10 and CM-100. I would appreciate your
contacting me if any of you have extra cassettes that you are willing to
give/sell to me.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



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From: TindallR-at-missouri.edu
Date: Tue, 12 Jul 2005 17:12:03 -0500
Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I have another one of my "back to the basics" questions that probably
make people wonder how the devil I ever got into this business in the
first place, but here goes anyway.

We have a client who prepares his own blocks and brings them to us for
sectioning, staining, viewing, etc. One day he came into the lab with a
bunch of sections on copper mesh grids which he had done
immunocytochemistry with standard gold-conjugated secondaries. After
viewing them, I offered him the standard advice that next time we would
mount some sections on nickel or gold grids if he let us know in advance
that he would be doing immunocytochemistry. He said he always used
copper and asked why he should switch. My answer was something like
"Umm-uhhh.....because it's always done that way" with an additional
mumble about copper interfering with the labeling reaction somehow.

Now I have another client, also infinitely more savvy in chemistry that
I am, asking the same question.

So I checked through our little in-house research library (again) and
did some targeted Googling (again) and found that some folks say that
copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
that anyway); 2) may react with PBS buffers to produce fine
precipitates (but, but....doesn't that mean we should NEVER use Cu with
PBS?); 3) reacts with gold colloid (no other explanation given), 4)
can alter the charge distribution of the section and cause non-specific
background label; and 5) may be oxidized during labelling or interfere
with oxidation of chemical groups in the tissue to be labelled. Mostly
people just say to use Ni or Au without stating any reasons.

Also, a glance through the literature shows that it's not uncommon to
find perfectly successful immunolabelling done on copper grids.

Now, if I were determined to invade a country called Copper any or all
of the above reasons could be used with little further explanation, but
I'd like to know the Truth and pass it along to my admiring customers.

Any takers?

Thanks in advance.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: Rosemary.White-at-csiro.au
Date: Tue, 12 Jul 2005 17:53:35 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Randy,

I only ran into the "use only Ni or Au grids" rule here, in my current job,
and my predecessor certainly produced some superb images showing gold
labelling of TEM sections. Blissfully unaware of this rule, I had been
using copper grids for all EM immunolabelling. To get good labelling of one
particular structure, which is about 40 nm diameter, I used uncoated
thin-bar Cu grids so I could get labelling on both sides of the section -
seemed to work just fine. I'll be interested to see the responses to your
question.

cheers,
Rosemary

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia


} From: TindallR-at-missouri.edu
} Reply-To: microscopy-at-microscopy.com
} Date: Tue, 12 Jul 2005 17:15:38 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for
} sectioning, staining, viewing, etc. One day he came into the lab with a
} bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we would
} mount some sections on nickel or gold grids if he let us know in advance
} that he would be doing immunocytochemistry. He said he always used
} copper and asked why he should switch. My answer was something like
} "Umm-uhhh.....because it's always done that way" with an additional
} mumble about copper interfering with the labeling reaction somehow.
}
} Now I have another client, also infinitely more savvy in chemistry that
} I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause non-specific
} background label; and 5) may be oxidized during labelling or interfere
} with oxidation of chemical groups in the tissue to be labelled. Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all
} of the above reasons could be used with little further explanation, but
} I'd like to know the Truth and pass it along to my admiring customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original Headers==============================
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==============================Original Headers==============================
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From: hanke-at-mee-inc.com
Date: Tue, 12 Jul 2005 18:02:49 -0500
Subject: [Microscopy] Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers:

As usual, I am hoping to enlist the vast knowledge and experience of the
this group to solve a perplexing problem. Here is the puzzle.

We have noticed artifact elemental peaks in EDS spectra obtained with
our Oxford Inca system on a Hitachi S-4700 FESEM. For example, an
analysis in the center of a strip of 6 mm wide clean copper tape on an
aluminum stub generates a spectrum with large peaks for copper (this is
good) and a small aluminum peak (not good). Repeat the experiment with a
carbon stub, and the small aluminum peak is replaced by a carbon peak.

Oddly, the phenomenon is observed only with the microscope operating in
High Mag mode. The same analysis (same mag, count rate, etc.) on the
copper tape in Low Mag mode detects only copper. This result is similar
to what we might expect from beam scatter in a variable pressure SEM.

Anyone one out there experienced this problem? Anyone with a similar
microscope willing to try the copper tape experiment to let us know if
this is unique to our instrument or fundamental to the S-4700. Hitachi
and Oxford are perplexed so far.

Thanks for your kind assistance.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


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From: Sally.Stowe-at-anu.edu.au
Date: Tue, 12 Jul 2005 18:07:04 -0500
Subject: [Microscopy] RE: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,
I had problems with Cu grids when incubating sections for long periods -
overnight for instance. The copper would react with whatever was available
and form green-blue salts. Not unexpected. We switched to nickel grids for
a while but they charge, gold but they are delicate...by this time we had
shortened the incubation times used, and tried copper again... And had no
problem using coated grids and incubation times of an hour or so. Grids are
floated on drops of media so that only the coated side is wetted - I don't
know if that is important.

Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
ANU CRICOS#00120C



} -----Original Message-----
} From: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
} Sent: Wednesday, 13 July 2005 8:12 AM
} To: sally.stowe-at-anu.edu.au
} Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids
}
}
}
}
}
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From: gary-at-gaugler.com
Date: Tue, 12 Jul 2005 18:30:50 -0500
Subject: [Microscopy] Re: Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What is the analytical WD for EDS in this SEM?
What WD are you using?

gary g.


At 04:04 PM 7/12/2005, you wrote:



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From: dsherman-at-purdue.edu
Date: Tue, 12 Jul 2005 19:08:23 -0500
Subject: [Microscopy] Film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike,

I didn't think the ones from the EM series would work in the CM series. Our
EM-400 took the larger plate cassettes with the film inserts. I thought
those inserts were different from the film holders that came with the CM
series but I may be wrong. If they do work than I may still have a few
around as well. I gave away most of my cameras and cassettes from the
EM-400 when we decommissioned it but still may have a few around. I'll check
and let you know if I would like to try the ones you have.

Are you going to M&M2005?

Debby


On 7/12/05 1:40 PM, "mgoheen-at-iupui.edu" {mgoheen-at-iupui.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Hi Debby,
}
} I got quite a few from our Philips 300 before we got rid of it. How many
} do you need? I use them to replace bad ones in our CM10 and they work
} fine.
}
} Mike
}
} Michael P. Goheen
} Electron Microscopy Lab
} Dept. of Pathology & Lab Medicine
} Indiana University School of Medicine
} Tel. 317-274-7604
} Fax 317-274-5346
} mgoheen-at-iupui.edu
}
}
} -----Original Message-----
} X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
} Sent: Tuesday, July 12, 2005 12:04 PM
} To: Goheen, Michael P.
} Subject: [Microscopy] Film cassettes
}
}
}
}
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} America
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} ------------------------------------------------------------------------
} ----
}
} Folks,
}
} Due to numerous camera jams over the years we are a bit short on film
} cassettes for the Philips CM-10 and CM-100. I would appreciate your
} contacting me if any of you have extra cassettes that you are willing to
} give/sell to me.
}
} Thanks,
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy
}
}
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} Headers==============================
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From: colijn.1-at-osu.edu
Date: Tue, 12 Jul 2005 19:12:25 -0500
Subject: [Microscopy] Re: Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Larry,

I'm not overly familiar with the Hitachi SEMs, but is the "hi-mag" mode on
your SEM an immersion lens mode? This is most likely the case if you are
using an "in-lens" or "in-column" detector. If so, it is possible that the
BSE are being bent around by the immersion field and striking back on the
sample. Depending on the immersion field, this could be close or far from
the beam. These will probably still be within the viewing angle of your
EDS collimator. In their immersion lens SEMs, FEI provides an EDS mode
with a weakened immersion lens field so that the BSE are bent somewhat less
and strike outside the field of view of the EDS collimator.

Cheers,
Henk


At 07:04 PM 7/12/2005, hanke-at-mee-inc.com wrote:



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From: colijn.1-at-osu.edu
Date: Tue, 12 Jul 2005 19:24:12 -0500
Subject: [Microscopy] Re: Film cassettes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debbie,

The CM series used both the 36 and 56 sheet film boxes. We have both an
early CM12 which used the 2-part film carriers (36 exposure box; same as
our old EM400) and a CM200 with the thinner single piece film carriers (56
exposure box). I don't know if the EM300 used the same film carriers as
the EM400 series since the camera boxes in the EM300 we had (years ago)
were different. The EM400 and later Philips/FEI scopes use the single
"behind the column" film box assembly

I believe the issue is more the film box than the microscope. ...and more
specifically, the film transport tray since the 2 carriers are different
thicknesses. The boxes have the same outside dimensions and are (I
believe) interchangeable. In other words, I could take my old 36 exposure
film box, drop it right into my CM200 and only need to change the film
stock number. CAUTION -- I haven't actually tested this since I haven't
felt motivated to reduce the number of exposures in my microscope!

Cheers,
Henk


At 08:10 PM 7/12/2005, dsherman-at-purdue.edu wrote:

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040 Fontana Labs, 116 W. 19th Ave


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From: dsherman-at-purdue.edu
Date: Tue, 12 Jul 2005 19:46:33 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

We used Cu grids for many years. Normally we use formvar + carbon coated
grids for ICC since we have a lot less loss of sections. We also tend to do
long incubations ...usually overnight. This is time efficient and also lets
us use highly diluted antibody so also minimize background due to
cross-reactions from contaminants in polyclonal antibodies.

Occasionally we would have a reaction due to copper oxidizing with resultant
green solution. I attributed this to salts or traces of Tween 20 in the
buffer and breaks in the coating exposing the Cu. As far as I can see, this
is the only reason for not using Cu grids if you use coated grids. We have
switched for the most part to using coated Ni grids to avoid this problem.

On the other hand, occasionally we need to do a double labeling using
uncoated grids and both sides of the sections. I would hesitate to use Cu
for this and prefer Ni. Gold would work but is both more expensive and more
delicate than Ni grids.

Debby


Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 7/12/05 5:15 PM, "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} wrote:

}
}
}
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} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for
} sectioning, staining, viewing, etc. One day he came into the lab with a
} bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we would
} mount some sections on nickel or gold grids if he let us know in advance
} that he would be doing immunocytochemistry. He said he always used
} copper and asked why he should switch. My answer was something like
} "Umm-uhhh.....because it's always done that way" with an additional
} mumble about copper interfering with the labeling reaction somehow.
}
} Now I have another client, also infinitely more savvy in chemistry that
} I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause non-specific
} background label; and 5) may be oxidized during labelling or interfere
} with oxidation of chemical groups in the tissue to be labelled. Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all
} of the above reasons could be used with little further explanation, but
} I'd like to know the Truth and pass it along to my admiring customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original Headers==============================
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12, 21 -- Subject: Re: [Microscopy] TEM: Immunocytochemistry and Cu grids
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From: paul_hazelton-at-umanitoba.ca
Date: Tue, 12 Jul 2005 22:55:26 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The film holders for the Philips EM400 and the CM series are the same. The
same holders can be used in the Philips EM201,EM300, EM301 . With the
inserts
you load cut film without you load glass plates.
----- Original Message -----
X-from: {dsherman-at-purdue.edu}
To: {amtecss-at-earthlink.net}
Sent: Tuesday, July 12, 2005 8:08 PM

randy

you don't say what your client's stuff looked like. that would help.

had a former director, before i grew up and went back to school - or was
that truly growing up? anyway, out of nickel grids, not to mention
formvar-nickel. she wanted IEM done. then! forthwith! no delay! do
not put it of! threat of discipline if not in her hands that
afternoon!! etc. insisted i use copper. this was also on formvar
coated grids. not pretty, quite nasty, large semicrystals of junk all
over. really did not look good after the second and third try.

there were several possible problems. first, we spun things onto the
grid, 30 minutes in an air driven ultracentrifuge. so the copper back
surface was guaranteed to get wet and react with the buffer. or perhaps
it was the buffer she had things in, i never could get a straight answer
from her on that one.

recently i did a long ultracentrifuge run to load some 10S particles
onto a formvar-copper grid. the grids also reacted with the phosphate
buffer over the 4 hour spin time. again, not a pretty picture - i had
to go back to my collaborator and get fresh material so we could put it
onto formvar-nickel grids. wonderful gentleman. much more mature.
real pleasure to collaborate with.

having said that, i read what rosemary white, sally stowe and debbie
sherman have said. i make no pretense about my opinion on dogma
(dogma-schmogma, it ain't gospel until i try it and prove the fact to me
personally). give it a go and let the rest of us try out to see what
happens. perhaps i will even try it in my overworked 10 finger lab.
but after the two molecular experiments i need to do in order get that
paper of mine accepted on resubmission, and the 7 other collaborators'
projects, the looking at micrographs from 2 outside sources with
questions, etc. all right, i exagerate, it's not quite that bad but i
really do have to get back to one person about his results before i go
on holiday tomorrow.

give it a shot and let us know

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926


==============================Original Headers==============================
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From: eprincipe01-at-hotmail.com
Date: Tue, 12 Jul 2005 23:22:57 -0500
Subject: [Microscopy] Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

With magnetic field active, test stray signal as a function of WD. Same experiment with a charge sensitive sample should also be interesting, not for x-ray, but SE. response.




-----Original Message-----
X-from: "hanke-at-mee-inc.com" {hanke-at-mee-inc.com}
Sent: Tuesday, July 12, 2005 11:03 PM
To: "eprincipe01-at-hotmail.com" {eprincipe01-at-hotmail.com}

Dear Listers:

As usual, I am hoping to enlist the vast knowledge and experience of the
this group to solve a perplexing problem. Here is the puzzle.

We have noticed artifact elemental peaks in EDS spectra obtained with
our Oxford Inca system on a Hitachi S-4700 FESEM. For example, an
analysis in the center of a strip of 6 mm wide clean copper tape on an
aluminum stub generates a spectrum with large peaks for copper (this is
good) and a small aluminum peak (not good). Repeat the experiment with a
carbon stub, and the small aluminum peak is replaced by a carbon peak.

Oddly, the phenomenon is observed only with the microscope operating in
High Mag mode. The same analysis (same mag, count rate, etc.) on the
copper tape in Low Mag mode detects only copper. This result is similar
to what we might expect from beam scatter in a variable pressure SEM.

Anyone one out there experienced this problem? Anyone with a similar
microscope willing to try the copper tape experiment to let us know if
this is unique to our instrument or fundamental to the S-4700. Hitachi
and Oxford are perplexed so far.

Thanks for your kind assistance.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


==============================Original Headers==============================
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From: YANGA-at-AGR.GC.CA
Date: Wed, 13 Jul 2005 08:24:44 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I had a bad experience with formvar-copper grids in immunogold experiment many years ago when incubating primary antibody overnight and the rest done on the following morning. I am using Nickel grids now -- no more problems.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
yanga-at-agr.gc.ca

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
X-from: Rosemary.White-at-csiro.au [mailto:Rosemary.White-at-csiro.au]
Sent: Tuesday, July 12, 2005 6:55 PM
To: Yang, Ann-Fook

Dear Randy,

I only ran into the "use only Ni or Au grids" rule here, in my current job,
and my predecessor certainly produced some superb images showing gold
labelling of TEM sections. Blissfully unaware of this rule, I had been
using copper grids for all EM immunolabelling. To get good labelling of one
particular structure, which is about 40 nm diameter, I used uncoated
thin-bar Cu grids so I could get labelling on both sides of the section -
seemed to work just fine. I'll be interested to see the responses to your
question.

cheers,
Rosemary

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia


} From: TindallR-at-missouri.edu
} Reply-To: microscopy-at-microscopy.com
} Date: Tue, 12 Jul 2005 17:15:38 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for
} sectioning, staining, viewing, etc. One day he came into the lab with a
} bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we would
} mount some sections on nickel or gold grids if he let us know in advance
} that he would be doing immunocytochemistry. He said he always used
} copper and asked why he should switch. My answer was something like
} "Umm-uhhh.....because it's always done that way" with an additional
} mumble about copper interfering with the labeling reaction somehow.
}
} Now I have another client, also infinitely more savvy in chemistry that
} I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause non-specific
} background label; and 5) may be oxidized during labelling or interfere
} with oxidation of chemical groups in the tissue to be labelled. Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all
} of the above reasons could be used with little further explanation, but
} I'd like to know the Truth and pass it along to my admiring customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original Headers==============================
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From: gwe-at-ufl.edu
Date: Wed, 13 Jul 2005 08:39:16 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have had problems with Cu grids, even Formvar coated ones. I have
precoated copper grids with Parlodion by dipping, blotting and drying
before use, with or with out a formvar coating. THat method seems to
protect the copper from reacting with PBS or whatever. This is not
original to me, but I cannot recall where I read this (among many other
things I can no longer recall). I think it might have been one of those
smart Swiss guys.

Greg

TindallR-at-missouri.edu wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
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From: marc.pypaert-at-yale.edu
Date: Wed, 13 Jul 2005 10:37:12 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What's the big deal about preferring Cu to Ni anyway?
Right, there is the charging problem with Nickel, but if
you use anti-magnetic tweezers they are just as easy
to handle than copper. And the price is really not that
much more! We sometimes have to leave grids
overnight or longer on solutions, so to be safe we use
nickel for this. Since we don't want to make and keep
separate stocks of copper and nickel, we switched
entirely to nickel grids, and have had no problems with
any of our applications. Am I missing something here?!!

Marc

On Tuesday, July 12, 2005, at 06:13 PM, TindallR-at-missouri.edu wrote:

}
}
}
} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} -----
}
} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for
} sectioning, staining, viewing, etc. One day he came into the lab with
} a
} bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we would
} mount some sections on nickel or gold grids if he let us know in
} advance
} that he would be doing immunocytochemistry. He said he always used
} copper and asked why he should switch. My answer was something like
} "Umm-uhhh.....because it's always done that way" with an additional
} mumble about copper interfering with the labeling reaction somehow.
}
} Now I have another client, also infinitely more savvy in chemistry that
} I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause non-specific
} background label; and 5) may be oxidized during labelling or
} interfere
} with oxidation of chemical groups in the tissue to be labelled.
} Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all
} of the above reasons could be used with little further explanation, but
} I'd like to know the Truth and pass it along to my admiring customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original
} Headers==============================
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--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original Headers==============================
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From: TindallR-at-missouri.edu
Date: Wed, 13 Jul 2005 10:56:21 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marc,

It doesn't make a lot of difference to me either way, except to be able
to actually have an intelligent explanation when our users ask questions
about our methods. That said, there are times when we do get severe
image distortion in our TEM when using Ni grids with a healthy charge on
them. But my main motivation was curiosity, nada mas.

Randy

-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Wednesday, July 13, 2005 10:39 AM
To: Tindall, Randy D.

What's the big deal about preferring Cu to Ni anyway?
Right, there is the charging problem with Nickel, but if you use
anti-magnetic tweezers they are just as easy to handle than copper. And
the price is really not that much more! We sometimes have to leave grids
overnight or longer on solutions, so to be safe we use nickel for this.
Since we don't want to make and keep separate stocks of copper and
nickel, we switched entirely to nickel grids, and have had no problems
with any of our applications. Am I missing something here?!!

Marc

On Tuesday, July 12, 2005, at 06:13 PM, TindallR-at-missouri.edu wrote:

}
}
}
} ----------------------------------------------------------------------
} -
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} ----------------------------------------------------------------------
} -
} -----
}
} Dear Listers,
}
} I have another one of my "back to the basics" questions that probably
} make people wonder how the devil I ever got into this business in the
} first place, but here goes anyway.
}
} We have a client who prepares his own blocks and brings them to us for

} sectioning, staining, viewing, etc. One day he came into the lab with

} a bunch of sections on copper mesh grids which he had done
} immunocytochemistry with standard gold-conjugated secondaries. After
} viewing them, I offered him the standard advice that next time we
} would mount some sections on nickel or gold grids if he let us know in

} advance that he would be doing immunocytochemistry. He said he always

} used copper and asked why he should switch. My answer was something
} like "Umm-uhhh.....because it's always done that way" with an
} additional mumble about copper interfering with the labeling reaction
} somehow.
}
} Now I have another client, also infinitely more savvy in chemistry
} that I am, asking the same question.
}
} So I checked through our little in-house research library (again) and
} did some targeted Googling (again) and found that some folks say that
} copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} that anyway); 2) may react with PBS buffers to produce fine
} precipitates (but, but....doesn't that mean we should NEVER use Cu
with
} PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} can alter the charge distribution of the section and cause
} non-specific background label; and 5) may be oxidized during
} labelling or interfere
} with oxidation of chemical groups in the tissue to be labelled.
} Mostly
} people just say to use Ni or Au without stating any reasons.
}
} Also, a glance through the literature shows that it's not uncommon to
} find perfectly successful immunolabelling done on copper grids.
}
} Now, if I were determined to invade a country called Copper any or all

} of the above reasons could be used with little further explanation,
} but I'd like to know the Truth and pass it along to my admiring
customers.
}
} Any takers?
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original
} Headers==============================
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--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original
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From: ehaller-at-hsc.usf.edu
Date: Wed, 13 Jul 2005 11:22:28 -0500
Subject: [Microscopy] RE: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

The main problem encountered with copper grids used for
immunocytochemistry or immunogold labeling is the reaction of the copper
with salts in the buffers used during labeling. This is a time-related
reaction, and can usually be avoided by having short labeling runs and
using grids with films on them. Gilder grids, available from major
microscope supply vendors, are gold-coated copper grids, and are not
that much more expensive than regular copper grids. These grids may be
the variety used by researchers who have reported having no problems
with their grids during their immuno runs.
I usually use nickel grids for my work, but have used
formvar-coated copper grids without problems for 1 day immuno runs so
long as the film is intact and as long as I don't get clumsy and end up
sinking my grids in my solutions (if I do sink them, I do a quick
distilled water rinse, blot the back of the grids with filter paper
until almost dry, then re-float them where I left off). As others have
shared, the nickel grids are much sturdier, not too expensive, and are
not a problem to handle as long as you use antimagnetic forceps. With
the nickel grids, remember to correct for astigmatism in the TEM by
using a hole in your sample on the nickel grid. This will accommodate
for any inherent residual magnetic field in the grid itself.

Edward Haller
Lab Manager, Diagnostic Electron Microscopy Lab
University of South Florida
Pathology Department
Tampa, FL 33612
(813) 974-9584


==============================Original Headers==============================
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From: marc.pypaert-at-yale.edu
Date: Wed, 13 Jul 2005 12:01:03 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,

Sorry for any misunderstanding - My response was not
really directed at you, but at the EM community in general
who for some reason seems to be biased towards copper.
I have never understood this. I have been doing EM for
nearly 20 years, exclusively using nickel grids, and would
really like to find out if I was mistaken this entire time!! Or
is it just another case of "dogma", like Paul put in nicely
in his posting?
Anyway, I will read with interest the responses you get
to your posting!
Best

Marc

On Wednesday, July 13, 2005, at 11:57 AM, TindallR-at-missouri.edu wrote:

}
}
}
} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} -----
}
} Marc,
}
} It doesn't make a lot of difference to me either way, except to be able
} to actually have an intelligent explanation when our users ask
} questions
} about our methods. That said, there are times when we do get severe
} image distortion in our TEM when using Ni grids with a healthy charge
} on
} them. But my main motivation was curiosity, nada mas.
}
} Randy
}
} -----Original Message-----
} X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
} Sent: Wednesday, July 13, 2005 10:39 AM
} To: Tindall, Randy D.
} Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids
}
}
}
}
} -----------------------------------------------------------------------
} -
} ----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
} -
} ----
}
} What's the big deal about preferring Cu to Ni anyway?
} Right, there is the charging problem with Nickel, but if you use
} anti-magnetic tweezers they are just as easy to handle than copper. And
} the price is really not that much more! We sometimes have to leave
} grids
} overnight or longer on solutions, so to be safe we use nickel for this.
} Since we don't want to make and keep separate stocks of copper and
} nickel, we switched entirely to nickel grids, and have had no problems
} with any of our applications. Am I missing something here?!!
}
} Marc
}
} On Tuesday, July 12, 2005, at 06:13 PM, TindallR-at-missouri.edu wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------
} } -
} } -----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } -
} } -----
} }
} } Dear Listers,
} }
} } I have another one of my "back to the basics" questions that probably
} } make people wonder how the devil I ever got into this business in the
} } first place, but here goes anyway.
} }
} } We have a client who prepares his own blocks and brings them to us for
}
} } sectioning, staining, viewing, etc. One day he came into the lab with
}
} } a bunch of sections on copper mesh grids which he had done
} } immunocytochemistry with standard gold-conjugated secondaries. After
} } viewing them, I offered him the standard advice that next time we
} } would mount some sections on nickel or gold grids if he let us know in
}
} } advance that he would be doing immunocytochemistry. He said he always
}
} } used copper and asked why he should switch. My answer was something
} } like "Umm-uhhh.....because it's always done that way" with an
} } additional mumble about copper interfering with the labeling reaction
} } somehow.
} }
} } Now I have another client, also infinitely more savvy in chemistry
} } that I am, asking the same question.
} }
} } So I checked through our little in-house research library (again) and
} } did some targeted Googling (again) and found that some folks say that
} } copper: 1) reacts with Tris-HCL buffer (okay, but we hardly ever use
} } that anyway); 2) may react with PBS buffers to produce fine
} } precipitates (but, but....doesn't that mean we should NEVER use Cu
} with
} } PBS?); 3) reacts with gold colloid (no other explanation given), 4)
} } can alter the charge distribution of the section and cause
} } non-specific background label; and 5) may be oxidized during
} } labelling or interfere
} } with oxidation of chemical groups in the tissue to be labelled.
} } Mostly
} } people just say to use Ni or Au without stating any reasons.
} }
} } Also, a glance through the literature shows that it's not uncommon to
} } find perfectly successful immunolabelling done on copper grids.
} }
} } Now, if I were determined to invade a country called Copper any or all
}
} } of the above reasons could be used with little further explanation,
} } but I'd like to know the Truth and pass it along to my admiring
} customers.
} }
} } Any takers?
} }
} } Thanks in advance.
} }
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} }
} }
} }
} }
} }
} }
} }
} } ==============================Original
} } Headers==============================
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} }
} }
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original
} Headers==============================
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} 6, 18 -- Date: Wed, 13 Jul 2005 11:19:42 -0400 6, 18 -- From: Marc
} Pypaert {marc.pypaert-at-yale.edu} 6, 18 -- Subject: Re: [Microscopy] TEM:
} Immunocytochemistry and Cu grids 6, 18 -- In-reply-to:
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--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original Headers==============================
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From: tonygr-at-MIT.EDU
Date: Wed, 13 Jul 2005 12:32:00 -0500
Subject: [Microscopy] RE: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Over the years I have frequently seen cases where the samples themselves
have reacted with the copper grid. Two examples:

1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
precipitates were ugly and analyzed as copper sulphide. Our surmise was
that the iron and copper had undergone ion exchange while the grid (and
carbon film) was wet with the culture medium. When we used nickel grids,
the precipitates were well formed and composed of iron and sulphur.

2) Looking at Si precipitates in Al-Si electronic bond wire. The
specimens were prepared by embedding and microtoming, floating in DI
water. The Si precipitates were surrounded by an ugly mess containing
masses of copper. Again, presumed to me electrochemical reaction between
the Al and the Cu grid, through the DI water medium, somehow preferentially
at the Si precipitates. Again, use of Ni grids produced good pictures
allowing us to characterize the precipitation.

Having said all that, we continue to default to use copper grids!

Tony.


***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


==============================Original Headers==============================
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From: MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 13 Jul 2005 12:51:31 -0500
Subject: [Microscopy] RE: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for your weigh-in on this subject. I was sorry to learn of your hearing
problems; has this been coming on gradually or did something happen (i.e. a
severe cold, etc.)to bring the hearing loss on?

I was surprised to learn that Boston is not yet out of the running for an MSA
meeting! If I can help in any way, I'll throw my hat in the ring.

Hope your summer (now that it is the middle of July!) is going well.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
X-from: tonygr-at-MIT.EDU [mailto:tonygr-at-MIT.EDU]
Sent: Wednesday, July 13, 2005 1:33 PM
To: Sherwood, Margaret

Over the years I have frequently seen cases where the samples themselves
have reacted with the copper grid. Two examples:

1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
precipitates were ugly and analyzed as copper sulphide. Our surmise was
that the iron and copper had undergone ion exchange while the grid (and
carbon film) was wet with the culture medium. When we used nickel grids,
the precipitates were well formed and composed of iron and sulphur.

2) Looking at Si precipitates in Al-Si electronic bond wire. The
specimens were prepared by embedding and microtoming, floating in DI
water. The Si precipitates were surrounded by an ugly mess containing
masses of copper. Again, presumed to me electrochemical reaction between
the Al and the Cu grid, through the DI water medium, somehow preferentially
at the Si precipitates. Again, use of Ni grids produced good pictures
allowing us to characterize the precipitation.

Having said all that, we continue to default to use copper grids!

Tony.


***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


==============================Original Headers==============================
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From: tpepper-at-iastate.edu
Date: Wed, 13 Jul 2005 13:54:01 -0500
Subject: [Microscopy] viaWWW: Help with Fixative near Plainview Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(tpepper-at-iastate.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
on Wednesday, July 13, 2005 at 09:21:59
---------------------------------------------------------------------------

Email: tpepper-at-iastate.edu
Name: Tracey Pepper

Organization: Iowa State University

Title-Subject: [Filtered] MListserver:

Question: HELP! We are in desparate need of some assisitance! We have a scientist in Texas who is in the field collecting crucial samples for TEM examination. They must be collected in the next couple of days. Our efforts to send (via DHL) good fixative (standard em fix for plants a double aldehyde in a 0.1 M cacodylate) have failed! The person is located in Plainview, Texas. Is there anyone on this listserver who could make us some fixtive (150 mls)for this person to come pick up? Preferably within an hour radius? If you can, Please call 515-294-3872 as soon as possible. We would be in your debt!!
With Great appreciation,
Tracey Pepper
Bessey Microscopy Facility
Iowa State University
Ames, IA 50011-1020

---------------------------------------------------------------------------

==============================Original Headers==============================
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6, 12 -- Subject: viaWWW: Help with Fixative near Plainview Texas
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From: gcc-at-couger.com
Date: Wed, 13 Jul 2005 15:01:27 -0500
Subject: [Microscopy] Re: viaWWW: Help with Fixative near Plainview Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Your best bet would be to call some one at Texas Tech in Lubbock
http://www.macmed.ttuhsc.edu/ They may be more than an hour from
Plainview but that close to the edge of the world. Your only
other chance is Amarillo with the TAM Vet Med Diagnostic lab
http://www.medcenter.org/facilities/am-vetlab.html.

Good luck
Gordon

Gordon Couger
I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org

tpepper-at-iastate.edu wrote:
}
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It was submitted by
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} on Wednesday, July 13, 2005 at 09:21:59
}
---------------------------------------------------------------------------
}
} Email: tpepper-at-iastate.edu
} Name: Tracey Pepper
}
} Organization: Iowa State University
}
} Title-Subject: [Filtered] MListserver:
}
} Question: HELP! We are in desparate need of some
assisitance! We have a scientist in Texas who is in the field
collecting crucial samples for TEM examination. They must be
collected in the next couple of days. Our efforts to send (via
DHL) good fixative (standard em fix for plants a double aldehyde
in a 0.1 M cacodylate) have failed! The person is located in
Plainview, Texas. Is there anyone on this listserver who could
make us some fixtive (150 mls)for this person to come pick up?
Preferably within an hour radius? If you can, Please call
515-294-3872 as soon as possible. We would be in your debt!!
} With Great appreciation,
} Tracey Pepper
} Bessey Microscopy Facility
} Iowa State University
} Ames, IA 50011-1020
}
}
---------------------------------------------------------------------------
}
} ==============================Original
Headers==============================
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==============================Original Headers==============================
5, 20 -- From gcc-at-couger.com Wed Jul 13 15:01:27 2005
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From: andrew.mcnaughton-at-stonebow.otago.ac.nz
Date: Wed, 13 Jul 2005 15:43:43 -0500
Subject: [Microscopy] Nikon Diaphot Epi-Fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello

Does anyone have a Nikon Diaphot epi-fluorescence lamp house and filter
set/holder for sale? Alternatively, if anyone knows of a second hand
microscope parts vendor?

Thanks for your help.

Regards

Andrew



________________________________________________________________________
______
Andrew McNaughton
Otago Centre for Confocal Microscopy
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: +64 3 479 7308
Facsimile: +64 3 479 7254

Confocal/EM Centres: http://occm.otago.ac.nz/

Department: http://anatomy.otago.ac.nz/

New Zealand Microscopy: http://microscopynz.otago.ac.nz/

________________________________________________________________________
______


==============================Original Headers==============================
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From: sryazant-at-ucla.edu
Date: Wed, 13 Jul 2005 16:09:43 -0500
Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am using titanium tweezers with nickel grids - they are cheap nowadays
and I like them (tweezers) for light weight and soft action. I am using
carbon coating on top of all my plastic films (copper or nickel grids) and
have no problems with charge/astigmatism. I suspect, charging problem
happens when grids quite old and/or dirty. Sergey

At 09:23 AM 7/13/2005, you wrote:



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From: jfb-at-uidaho.edu
Date: Wed, 13 Jul 2005 16:27:34 -0500
Subject: [Microscopy] Re: viaWWW: Help with Fixative near Plainview

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John Oren, Vermontoptech 802 425 2040
----- Original Message -----
X-from: {andrew.mcnaughton-at-stonebow.otago.ac.nz}
To: {micro-at-superlink.net}
Sent: Wednesday, July 13, 2005 4:43 PM

Actually, TTU is only about 45 minutes from Plainview, and both the Dept. of
Bio. Sci. and the TTU Medical School have electron microscopy labs.

-----Original Message-----
X-from: gcc-at-couger.com [mailto:gcc-at-couger.com]
Sent: Wednesday, July 13, 2005 1:05 PM
To: jfb-at-uidaho.edu

Your best bet would be to call some one at Texas Tech in Lubbock
http://www.macmed.ttuhsc.edu/ They may be more than an hour from
Plainview but that close to the edge of the world. Your only
other chance is Amarillo with the TAM Vet Med Diagnostic lab
http://www.medcenter.org/facilities/am-vetlab.html.

Good luck
Gordon

Gordon Couger
I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org

tpepper-at-iastate.edu wrote:
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} Email: tpepper-at-iastate.edu
} Name: Tracey Pepper
}
} Organization: Iowa State University
}
} Title-Subject: [Filtered] MListserver:
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} Question: HELP! We are in desparate need of some
assisitance! We have a scientist in Texas who is in the field
collecting crucial samples for TEM examination. They must be
collected in the next couple of days. Our efforts to send (via
DHL) good fixative (standard em fix for plants a double aldehyde
in a 0.1 M cacodylate) have failed! The person is located in
Plainview, Texas. Is there anyone on this listserver who could
make us some fixtive (150 mls)for this person to come pick up?
Preferably within an hour radius? If you can, Please call
515-294-3872 as soon as possible. We would be in your debt!!
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From: r-holdford-at-ti.com
Date: Wed, 13 Jul 2005 17:39:30 -0500
Subject: [Microscopy] Stray X-rays in FE SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Henk: I believe you are correct. The "hi-mag" mode does use an in-lens
detector and you will get x-ray peaks you are not wanting due to the way
the magnetic lens acts.

Larry: using "Analysis" mode will correct this problem. The WD for
this scope with an INCA detector should be about 12mm +/- 1mm. Using
the "hi-mag" mode for EDX work is not recommended; you won't get good
resolution at this working distance anyway and the in-lens detector
won't give a very good image at this distance, besides being very
sensitive to charging.

colijn.1-at-osu.edu wrote:

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From: xuy-at-nih.gov
Date: Thu, 14 Jul 2005 08:26:00 -0500
Subject: [Microscopy] viaWWW: digital camera for my light microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The actual retail price of T221 in the summer of 2004 (when T221 was
available from regular retail sources) was only $4K. Proper video adapters
are $800 to $1,200, largely matter of taste. It is safe to say the whole
deal was approximately. $5K. Totally worth it IMO. The figures around $8K to
$10K discussed in this thread are MSRP, not retail prices.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
X-from: {Mike.Bode-at-soft-imaging.net}
To: {vitalylazar-at-att.net}
Sent: Friday, July 08, 2005 11:01 AM

Hi John,

The advantages besides the obvious superb display are:

1) the ability to display entire image at 100% zoom (pixel-to-pixel
sensor-to-monitor) without zoom or pan;

2) have such image and multiple toolbars and control boxes displayed on the
desktop without interfering with each other, plus an extra application or
two open at the same time, again, not hiding behind other open windows-
makes it much easier on the operator.

Works very well for 4 or 6 megapixels camera. Now, for larger sensor such as
11 or 16 megapixels, one would have to zoom out to 50% in order to see
entire image at once. Still much better than zooming down to 25% on a
regular monitor.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
X-from: {john.mardinly-at-intel.com}
To: {vitalylazar-at-att.net}
Sent: Friday, July 08, 2005 4:16 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (xuy-at-nih.gov) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, July 14, 2005 at 08:09:33
---------------------------------------------------------------------------

Email: xuy-at-nih.gov
Name: Yuhui Xu

Organization: Harvard Medical School

Title-Subject: [Filtered] MListserver:

Question: I am in the process of buying a digital camera for my light microscope ( Leica DM LB2), and would like to get your opinion as to which maker or model is a good choice in terms of resolution, stability, and ease of use, and of course the price.

Thank you.

---------------------------------------------------------------------------

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From: j.wittig-at-vanderbilt.edu
Date: Thu, 14 Jul 2005 08:32:41 -0500
Subject: [Microscopy] viaWWW: Exhibitor Tutorials at MM2005

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

Once again the MSA Education Committee has organized Exhibitor Demonstrations and Tutorials during the Microscopy and Microanalysis 2005 meeting. This will occur on Tuesday, August 2 starting at 5:00 pm in the Exhibit Hall. These mini-seminars and/or tutorial demonstrations are held in the booths of the participating companies after the Hall is closed to non-participants.

Reservations sheets with titles and descriptions will be at the MSA Education table in the MSA Mega Booth. When you sign up you will be issued a ticket, which you will need to renter the Hall after it is closed. You need to sign up no later than noon on Tuesday. The number of attendees is limited, so visit the MSA Education table soon, since the demonstrations get filled up quickly!

Here's a list of participating Exhibitors and titles:

http://mm2005.microscopy.org/MMExhibitorsTutorialsDemos.html


Jim Wittig
MSA Education Committee

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From: nholson-at-ucsd.edu
Date: Thu, 14 Jul 2005 13:52:19 -0500
Subject: [Microscopy] Illuminator for old Wild light microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Your problem is simply one of backscatter bouncing off the final lens
irradiating areas off axis, generating the x-rays as appropriate. This is
not just a problem with the SEM you mention, it is a general problem of
which many people are unaware. The worst case I have ever seen was
obtaining x-ray information from 0.75cm (~1/4inch) from the point of initial
beam impact!

Modern detectors and collimators do help but scatter is always a problem. I
believe that is why SEM manufacturer's main holder is often one which places
the specimen surface at a higher level well away from the holder/stage
interface. A specimen "in space" will always offer a cleaner x-ray signal.

It is a personal suggestion to clients that when trying hard with an
analysis they DO NOT use a multi specimen holder!

Those who wish to know more should read texts relating to the production of
SE in relation to those produced by "bounce off" backscatter. We have a
short piece in the "Hints and Tips" section of our web site.

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
X-from: {hanke-at-mee-inc.com}
To: {protrain-at-emcourses.com}
Sent: Wednesday, July 13, 2005 12:03 AM

We have an old Wild MTr19 illuminator for an equally old Wild model 20
compound light microscope. The bulb in the illuminator burnt out and from
the sources I checked that type of bulb is supposedly no longer made. Also,
there are no markings on the bulb to indicate what it is. Is anyone
familiar with this old illuminator and either knows of bulb replacements or
a compatible illuminator for which I can still get bulbs?

Thanks

Norm Olson
______________________________________________________________
Norm Olson
Cryoelectron Microscopy Facilities Manager
4107 Natural Science Building
Department of Chemistry & Biochemistry, MC-0378
University of California San Diego
La Jolla, CA 92093-0378
nholson-at-ucsd.edu
http://cryoem.ucsd.edu
(858)534-5852 ­ Office; (858)534-5846 - Fax
______________________________________________________________







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From: cpetty1-at-umbc.edu
Date: Thu, 14 Jul 2005 14:47:51 -0500
Subject: [Microscopy] Re: Illuminator for old Wild light microscope

Contents Retrieved from Microscopy Listserver Archives
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Joe Mears knows everything you what to know about the Swiss made Wild.
Microscope Services 1403 Bradley Avenue, Rockville, Maryland 20851
Office 301.294.7960 Fax 301.294.1934 Cell 240.994.7191

Joe also works on Leica, Zeiss, and Nikon light scopes. He is a very
good repair person.


nholson-at-ucsd.edu wrote:
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} We have an old Wild MTr19 illuminator for an equally old Wild model 20
} compound light microscope. The bulb in the illuminator burnt out and from
} the sources I checked that type of bulb is supposedly no longer made. Also,
} there are no markings on the bulb to indicate what it is. Is anyone
} familiar with this old illuminator and either knows of bulb replacements or
} a compatible illuminator for which I can still get bulbs?
}
} Thanks
}
} Norm Olson
} ______________________________________________________________
} Norm Olson
} Cryoelectron Microscopy Facilities Manager
} 4107 Natural Science Building
} Department of Chemistry & Biochemistry, MC-0378
} University of California San Diego
} La Jolla, CA 92093-0378
} nholson-at-ucsd.edu
} http://cryoem.ucsd.edu
} (858)534-5852 ­ Office; (858)534-5846 - Fax
} ______________________________________________________________
}
}
}
}
}
}
}
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} 9, 24 -- Subject: Illuminator for old Wild light microscope
} 9, 24 -- From: Norman Olson {nholson-at-ucsd.edu}
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--
Chere Petty, M.S.
Manager, Keith R. Porter Imaging Facility
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
1000 Hilltop Circle
Baltimore, MD 21250
Phone: 410-455-2296
Fax: 410-455-3875

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From: gary.m.brown-at-exxonmobil.com
Date: Thu, 14 Jul 2005 16:37:50 -0500
Subject: [Microscopy] Re: Illuminator for old Wild light microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

"The statements and opinions expressed here by Gary M. Brown represent
neither those of ExxonMobil Corporation nor its affiliates."


Norm,

Leica should have a part number for the bulb and the vital stats needed to
purchase it elsewhere.

Regards,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Technology & Engineering - West
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: 281 834 2387
fax: 281 834 2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



nholson-at-ucsd.e
du
To
gary.m.brown-at-exxonmobil.com
07/14/05 01:54 cc
PM
Subject
[Microscopy] Illuminator for old
Please respond Wild light microscope
to
microscopy-at-mic
roscopy.com










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We have an old Wild MTr19 illuminator for an equally old Wild model 20
compound light microscope. The bulb in the illuminator burnt out and from
the sources I checked that type of bulb is supposedly no longer made.
Also,
there are no markings on the bulb to indicate what it is. Is anyone
familiar with this old illuminator and either knows of bulb replacements or
a compatible illuminator for which I can still get bulbs?

Thanks

Norm Olson
______________________________________________________________
Norm Olson
Cryoelectron Microscopy Facilities Manager
4107 Natural Science Building
Department of Chemistry & Biochemistry, MC-0378
University of California San Diego
La Jolla, CA 92093-0378
nholson-at-ucsd.edu
http://cryoem.ucsd.edu
(858)534-5852 ­ Office; (858)534-5846 - Fax
______________________________________________________________







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From: john.bonevich-at-nist.gov
Date: Fri, 15 Jul 2005 12:11:10 -0500
Subject: [Microscopy] Gatan Duomill service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Does anyone know of a company that can service an old Gatan Duomill? Ours
has developed an electrical problem that keeps the guns from energizing.

Thanks in advance.

-----------------------------------------
John Bonevich, Ph.D.
National Institute of Standards and Technology
Metallurgy Division, Mail-Stop 8555
100 Bureau Drive
Gaithersburg, MD 20899 USA


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From: curulli-at-usc.edu
Date: Fri, 15 Jul 2005 12:43:29 -0500
Subject: [Microscopy] Gatan Duomill service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Try

MAS
Art McCanna
Suwanee, Georgia
800-421-8451

-----Original Message-----
X-from: john.bonevich-at-nist.gov [mailto:john.bonevich-at-nist.gov]
Sent: Friday, July 15, 2005 10:15 AM
To: curulli-at-usc.edu

Hello,

Does anyone know of a company that can service an old Gatan Duomill? Ours
has developed an electrical problem that keeps the guns from energizing.

Thanks in advance.

-----------------------------------------
John Bonevich, Ph.D.
National Institute of Standards and Technology
Metallurgy Division, Mail-Stop 8555
100 Bureau Drive
Gaithersburg, MD 20899 USA


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From: rcbaker-at-eden.infohwy.com
Date: Sat, 16 Jul 2005 10:57:37 -0500
Subject: [Microscopy] Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am an amateur and have been experimenting for quite some time with
a technique for sharpening silicon carbide (SiC) crystals as an
alternative to the very expensive diamond knives commonly used with
ultramicrotomes.

The major difficulty is that SiC crystals are very brittle; thus SiC
crystals need to be lapped and polished by unconventional methods to
prevent micro-mechanical stresses from breaking the edge.
Conventional gem faceting technology does not work.

Vitreous carbon knives have also been reported in the literature but
did not live up to expectations apparently.

As many on this list will know, glass knives are preferred for their
initial sharpness. Meanwhile diamond knives are preferred for their
unexcelled durability, flat faces and straight edges, which makes it
possible to make almost unlimited numbers of serial sections up to a
few millimeters wide.

My efforts indicate that it is possible to make SiC knives and that
they can generate silvery sections down to 100 nanometers or so,
which puts them in the ball park for practical EM work. My sections
do transmit electrons, and at best do not appear to be much worse
than diamond knives in regards to their initial sharpness.

Initially I had imagined that SiC knives might potentially replace
diamond knives, since the hardness of SiC crystals is 9+ on the Mho's
scale, while diamond is 10.

As it turns out, my SiC knives gradually become dull over the course
of making hundreds of sections. However they are distinctly more
durable than glass knives, and the lapped faces are much flatter and
the edges wider compared to glass.

The SiC knife durability seems to be a function of the hardness of
the embedding plastic being sectioned, as one may imagine. (They
section glycol methacrylate nicely, for making floating ribbons,
which is helpful since I intend to use the SiC knives myself for LM
work).

My question is how useful such knives are likely to be in practice? I
imagine SiC knives might fill a niche market for disposable
substitutes for diamond knives, when an inexpensive alternative to
glass knives is needed only occasionally and the purchase of a
diamond knife doesn't make sense, or maybe for student teaching.

It seems likely that the same technology can probably be used to make
sapphire knives of equivalent initial sharpness (sapphire is much
tougher and more fracture resistant than SiC but not so hard as SiC).
But I don't know, my efforts so far have only concerned SiC knives.

I expect to publish my SiC knife making technology and put it in the
public domain, but for now I am soliciting comments on their
potential usefulness. -- Roger, Austin, Tx







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From: luce-at-earthtech.org
Date: Sun, 17 Jul 2005 19:01:51 -0500
Subject: [Microscopy] AskAMicroscopist: schematics for an International Scientific

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Contact Art McCanna at MAS amccanna-at-mastest.com ; (770)866-3200;
direct-(770)866-3212. MAS is exclusive factory representative for GATAN ion
mills.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth, GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com

----- Original Message -----
X-from: {john.bonevich-at-nist.gov}
To: {vitalylazar-at-att.net}
Sent: Friday, July 15, 2005 1:13 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (luce-at-earthtech.org) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, July 17, 2005 at 16:06:51
---------------------------------------------------------------------------

Email: luce-at-earthtech.org
Name: George Luce

Organization: Earthtech Internaional, Inc.

Education: Graduate College

Location: Austin, Texas, USA

Question: We are looking for the electrical schematics for an International Scientific Instruments (ISI) Model 100B Scanning Electron Microscope. (~1979 vintage, probably made by Akashi)

Is this company still in business, or is there another resource for information about this SEM?

Thanks,

George Luce
luce-at-earthtech.org

Earthtech International Inc.
Austin, TX
www.earthtech.org



---------------------------------------------------------------------------

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From: pwje-at-sympatico.ca
Date: Sun, 17 Jul 2005 20:46:37 -0500
Subject: [Microscopy] Re: AskAMicroscopist: schematics for an International Scientific

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi George,
So you have a ISI 100B, I rep and service ISI SEMs in Canada but if I can be
of any help please ask.

To start with what is the problem you have?
I think I have the manual and schematics but they will cost you to get them
copied I'm afraid.

If you don't get any other offers let me know.
Cheers
Peter Earl
Toronto,Canada



==============================Original Headers==============================
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From: lcgould-at-med.cornell.edu
Date: Mon, 18 Jul 2005 09:11:25 -0500
Subject: [Microscopy] Re: Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roger

the only comment that I can make is that sapphire knives were marketed
a few (maybe 20) years ago but they were much more expensive than glass
and not as hard as diamond so were never really that popular. I suppose
in the real world a lot will depend on price and quality and whether
there is a sufficiently large market, now.

I wish you luck.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: rcbaker-at-eden.infohwy.com

Hi Roger,
It sounds interesting, keep us informed.
Years ago, Diatome did market a Sapphire knife as a less-expensive
alternative to diamonds. In my lab, they proved difficult to clean,
and did not wear well enough to continue using them. They seem to
have disappeared from the market, so my guess is that others had the
same observations.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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From: cammer-at-aecom.yu.edu
Date: Mon, 18 Jul 2005 09:14:33 -0500
Subject: [Microscopy] Re: vibrations in air cooled CCD cameras; follow-up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The problem has been solved by using external fans running through tubes to
the camera.

Roper and Cooke each have their own different designs for the fan. Each
were extremely helpful with the retrofits.

Cooke runs the camera power through the fan; this assures that the fan must
be on for the camera to run. Roper doesn't have this safety feature.

I would recommend not purchasing a camera with an on board fan. Always go
for external cooling.

Thanks for the help from everybody who responded to my query
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**


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From: murraytm-at-u.washington.edu
Date: Mon, 18 Jul 2005 10:56:23 -0500
Subject: [Microscopy] SEM Sample Prep Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a student who wants to look at clusters of methanosarcina and
methanosaeta in an aqueous solution. I have a materials background
and have never worked with this type of sample. The student found a
reference for preparing samples for electron microscopy which looks
to me like it is for TEM. She is interested in what the clusters
look like so is interested in retaining their relative position. I
think SEM would be a good technique to see the 3D cluster if there is
a method to prepare samples.

I have a FESEM which is high vacuum. I don't have easy access to an
environmental SEM, so I'm looking for a technique to image these
clusters in a high vacuum SEM.

Any suggestions for prep techniques and/or references?

Thanks,

Tom

------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email:
murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195


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From: jchandler-at-ial-fa.com
Date: Mon, 18 Jul 2005 11:51:41 -0500
Subject: [Microscopy] Training: looking for TEM short course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Try our new Quantomix Wet SEM Technology. Sounds like you have a perfect
application for the QX-102.

http://www.emsdiasum.com/microscopy/products/sem/wetsem.aspx?mm=11

Al Coritz, Technical Engineer
Electron Microscopy Sciences
www.emsdiasum.com


----- Original Message -----
X-from: {murraytm-at-u.washington.edu}
To: {Sampleprep-at-earthlink.net}
Sent: Monday, July 18, 2005 11:56 AM

A colleague of mine is looking for an intensive TEM short course, 3-4 days,
that covers theory and operation of TEM's, as well as interpretation of
images, particularly as they are used in materials science. The course at
Lehigh University for this year was just held, and waiting until next year
is not a good option.

If you teach or know about such a course, please contact me offline and I
will it forward the information.

Thanks very much,

--John Chandler
Fort Collins, CO
jchandler-at-ial-fa.com
970.217.1321


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From: peoshel-at-wisc.edu
Date: Mon, 18 Jul 2005 12:50:56 -0500
Subject: [Microscopy] Re: SEM Sample Prep Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom,

What's the procedure? Very likely, she can use it up through the 100%
ethanol steps, whether or not it is for TEM. The critters can be
filtered onto 0.22 micron membrane filters (the kind with nice, round
pores, not a Millipore), and the filter critical point dried.
An alternative to CPD is to air dry from HMDS (hexamethyldisilizane).
Process the samples in 1.5 mL minifuge tubes, not on filters. After
the last EtOH, go through a 2:1 1:1 1:2 EtOH:HMDS series, 3 changes
in 100% HMDS, put sputter coated filters on the SEM stubs, drop the
bugs in HMDS on the filters, and allow to air dry at room temp **do
all this in a fume hood!**.
See the U. Florida "tips and tricks" web site:
http://www.biotech.ufl.edu/EM/tips/index.html

Or have a chat with the U. Washington biological EM people.

Phil

} I have a student who wants to look at clusters of methanosarcina and
} methanosaeta in an aqueous solution. I have a materials background
} and have never worked with this type of sample. The student found a
} reference for preparing samples for electron microscopy which looks
} to me like it is for TEM. She is interested in what the clusters
} look like so is interested in retaining their relative position. I
} think SEM would be a good technique to see the 3D cluster if there is
} a method to prepare samples.
}
} I have a FESEM which is high vacuum. I don't have easy access to an
} environmental SEM, so I'm looking for a technique to image these
} clusters in a high vacuum SEM.
}
} Any suggestions for prep techniques and/or references?
}
} Thanks,
}
} Tom
}
} ------------------------------------------------------------------------
} ---------------------------------------------
} Thomas M Murray email:
} murraytm-at-u.washington.edu
} Electron Microscopy Center Manager Phone: (206)543-2836
} Materials Science & Engineering Fax: (206)543-3100
} Box 352120 302 Roberts Hall Cell: (425)345-0083
} University of Washington
} Seattle, WA 98195
}
}
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm

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From: bozzola-at-siu.edu
Date: Mon, 18 Jul 2005 14:49:53 -0500
Subject: [Microscopy] Re: Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HI ROGER,

One potential use would be to section materials that might damage a
diamond knife, but that could not be cut using a glass knife. For
example, specimens containing potentially damaging inclusions as
sand, metals, etc. or to cut hard botanical specimens.
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
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From: JOHN.WHEATLEY-at-asu.edu
Date: Mon, 18 Jul 2005 14:59:32 -0500
Subject: [Microscopy] RE: Training: looking for TEM short course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

We have a Winter School from January 9 through 12. One half day on the 13th. Please go to our web site where you will find a description of last year's school. We will have the info for 2006 up in a few days. Let me know if you need more info. John Wheatley


http://www.asu.edu/clas/csss/workshops/HREMschool.html



} ----------
} From: jchandler-at-ial-fa.com
} Reply To: microscopy-at-microscopy.com
} Sent: Monday, July 18, 2005 9:53 AM
} To: John Wheatley
} Subject: [Microscopy] Training: looking for TEM short course
}
}
}
}
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} A colleague of mine is looking for an intensive TEM short course, 3-4 days,
} that covers theory and operation of TEM's, as well as interpretation of
} images, particularly as they are used in materials science. The course at
} Lehigh University for this year was just held, and waiting until next year
} is not a good option.
}
} If you teach or know about such a course, please contact me offline and I
} will it forward the information.
}
} Thanks very much,
}
} --John Chandler
} Fort Collins, CO
} jchandler-at-ial-fa.com
} 970.217.1321
}
}
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From: TindallR-at-missouri.edu
Date: Mon, 18 Jul 2005 16:21:32 -0500
Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John,

Thanks so much for getting back to me on your training course. I had looked
at your website before polling the microscopy listserv, and it seemed to
have lots of what is needed. ASU is one of the few centers for EM that I
looked at before asking the question. I will pass along your information.

With best regards,

--John | jchandler-at-ial-fa.com | 970.217.1321

----- Original Message -----
X-from: {JOHN.WHEATLEY-at-asu.edu}
To: {jchandler-at-ial-fa.com}
Sent: Monday, July 18, 2005 1:59 PM

Dear Listers,

Below is a compilation of the replies I received to my recent question
about Cu vs. Ni grids for immunolabeling. I usually don't do this
without express permission, but most everybody posted to the list
anyway, and I made sure to edit those that didn't. If it bothers
anyone, let me know and I will never do it again.

In summary, it appears that copper can and will react with salts in
buffers and other solutions, although some people still have decent
luck. This problem can be ameliorated by using coated Cu grids and only
wetting the coated side and by shortening incubation times

Also, Marc Pypaert had the interesting suggestion of just using Ni grids
for everything, since they're not much more expensive, thereby avoiding
the problem entirely. Any thoughts on this?

Many thanks to everyone who replied! I now have a much better response
to our clients' questions on this matter (not to mention my own).

Cheers,
Randy


Over the years I have frequently seen cases where the samples themselves
have reacted with the copper grid. Two examples:

1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
precipitates were ugly and analyzed as copper sulphide. Our surmise was
that the iron and copper had undergone ion exchange while the grid (and
carbon film) was wet with the culture medium. When we used nickel
grids, the precipitates were well formed and composed of iron and
sulphur.

2) Looking at Si precipitates in Al-Si electronic bond wire. The
specimens were prepared by embedding and microtoming, floating in DI
water. The Si precipitates were surrounded by an ugly mess containing
masses of copper. Again, presumed to me electrochemical reaction
between the Al and the Cu grid, through the DI water medium, somehow
preferentially at the Si precipitates. Again, use of Ni grids produced
good pictures allowing us to characterize the precipitation.

Having said all that, we continue to default to use copper grids!

Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA


-------------------------------------------

The main problem encountered with copper grids used for
immunocytochemistry or immunogold labeling is the reaction of the copper
with salts in the buffers used during labeling. This is a time-related
reaction, and can usually be avoided by having short labeling runs and
using grids with films on them. Gilder grids, available from major
microscope supply vendors, are gold-coated copper grids, and are not
that much more expensive than regular copper grids. These grids may be
the variety used by researchers who have reported having no problems
with their grids during their immuno runs.
I usually use nickel grids for my work, but have used
formvar-coated copper grids without problems for 1 day immuno runs so
long as the film is intact and as long as I don't get clumsy and end up
sinking my grids in my solutions (if I do sink them, I do a quick
distilled water rinse, blot the back of the grids with filter paper
until almost dry, then re-float them where I left off). As others have
shared, the nickel grids are much sturdier, not too expensive, and are
not a problem to handle as long as you use antimagnetic forceps. With
the nickel grids, remember to correct for astigmatism in the TEM by
using a hole in your sample on the nickel grid. This will accommodate
for any inherent residual magnetic field in the grid itself.

Edward Haller
Lab Manager, Diagnostic Electron Microscopy Lab University of South
Florida Pathology Department Tampa, FL 33612

------------------------------------------------------------

We have had problems with Cu grids, even Formvar coated ones. I have
precoated copper grids with Parlodion by dipping, blotting and drying
before use, with or with out a formvar coating. THat method seems to
protect the copper from reacting with PBS or whatever. This is not
original to me, but I cannot recall where I read this (among many other
things I can no longer recall). I think it might have been one of those
smart Swiss guys.

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program Scientific Director, Electron
Microscopy P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
-------------------------------------------------------------------

I had a bad experience with formvar-copper grids in immunogold
experiment many years ago when incubating primary antibody overnight and
the rest done on the following morning. I am using Nickel grids now --
no more problems.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
------------------------------------------------------------------------
------------

recently i did a long ultracentrifuge run to load some 10S particles
onto a formvar-copper grid. the grids reacted with the phosphate buffer
over the 4 hour spin time. not a pretty picture - i had to go back to
my collaborator and get fresh material so we could put it onto
formvar-nickel grids. wonderful gentleman.


Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
----------------------------------------------------------------

We used Cu grids for many years. Normally we use formvar + carbon
coated grids for ICC since we have a lot less loss of sections. We also
tend to do long incubations ...usually overnight. This is time
efficient and also lets us use highly diluted antibody so also minimize
background due to cross-reactions from contaminants in polyclonal
antibodies.

Occasionally we would have a reaction due to copper oxidizing with
resultant green solution. I attributed this to salts or traces of Tween
20 in the buffer and breaks in the coating exposing the Cu. As far as I
can see, this is the only reason for not using Cu grids if you use
coated grids. We have switched for the most part to using coated Ni
grids to avoid this problem.

On the other hand, occasionally we need to do a double labeling using
uncoated grids and both sides of the sections. I would hesitate to use
Cu for this and prefer Ni. Gold would work but is both more expensive
and more delicate than Ni grids.

Debby Sherman, Manager
Life Science Microscopy Facility
Purdue University
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
------------------------------------------------------------------------
-

X-from my experience, coated copper grids will be fine for most (if not
all) immunolabelling, providing the solutions do not wet the uncoated
side of the grid. Uncoated copper grids always have been fine when I've
used PBS.

However, copper grids (uncoated - or the uncoated side) usually will
react with Tris-HCl buffers and some of the high salt buffer
formulations that I've used. Longer incubation times (eg. overnight)
cause more reaction with the grid than shorter times (eg. 60mins) - so
you may be able to use copper grids without problems if incubations are
short, even with buffer formulations that might react with the grid if
left for longer periods. It also is more difficult to prevent wetting
of the reverse (uncoated) side of coated copper grids if incubation
times are long, and particularly if wetting agents are used in the
buffer formulation.

When I started immunolabelling in the early 1980s - and hadn't yet heard
about the use of nickel grids - I used uncoated copper grids (and PBS)
for years without problems. My current lab generally uses a high salt
Tris-HCl buffer which includes a small amount of Tween 20, so we
routinely use nickel grids for immunolabelling. Nickel grids do have
some disadvantages (eg. charge), but we run a multi-user facility, often
training students or other inexperienced users, we've found nickel the
best option to overcome potential "grid-reaction" problems. However,
nickel grids certainly aren't essential for successful immunolabelling -
you just need to be aware of the possible problems.

Dr Deborah Stenzel
Analytical Electron Microscopy Facility
Queensland University of Technology
GPO Box 2434
Brisbane 4001
Australia

------------------------------------------------

I know (from sad experience) that Tris buffers will etch Cu grids - the
antibody droplets turn a lovely blue and there is gunk all over the
sections. I use Ni grids, only because I prefer Tris buffers (no real
reason why, I guess) for IEM and my current PI cringes at buying Au
grids.
I know one person who does all of her labelling in phosphate buffer and
uses Cu grids with no precipitate problems.

As for the other reasons you Googled....I've heard them, but never seen
any documentation nor had personal experience.

Can't wait to hear if anyone has actual evidence for some of the other
no-Cu reasons!

Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
----------------------------------------------------------------

Hi Randy,
I had problems with Cu grids when incubating sections for long periods -
overnight for instance. The copper would react with whatever was
available and form green-blue salts. Not unexpected. We switched to
nickel grids for a while but they charge, gold but they are
delicate...by this time we had shortened the incubation times used, and
tried copper again... And had no problem using coated grids and
incubation times of an hour or so. Grids are floated on drops of media
so that only the coated side is wetted - I don't know if that is
important.

Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
ANU CRICOS#00120C


I only ran into the "use only Ni or Au grids" rule here, in my current
job, and my predecessor certainly produced some superb images showing
gold labelling of TEM sections. Blissfully unaware of this rule, I had
been using copper grids for all EM immunolabelling. To get good
labelling of one particular structure, which is about 40 nm diameter, I
used uncoated thin-bar Cu grids so I could get labelling on both sides
of the section - seemed to work just fine. I'll be interested to see
the responses to your question.

Dr. Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia








Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: sryazant-at-ucla.edu
Date: Mon, 18 Jul 2005 17:44:32 -0500
Subject: [Microscopy] Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Roger
I am not sure is tungsten carbide is similar to SiC or not. My experience
with tungsten carbide basically was negative - this knife starts scratching
the surface after just 50+ 0.5 um sections of standard plastic embedded
tissue (no hard inclusions etc). As manufacturer explained to me it's
because of polycrystalline nature of the material - small crystals just
became loose and left the edge creating ruff surface. From this point of
view, amorphous (like glass) or monocrystal (like diamond) material is
preferable for good sections. Sapphire (hardness is 9) knifes were on the
market for while without great success. From economical point of view,
tungsten carbide knifes were also not so good - $100 each with ability to
produce only 50+ good sections. If SiC acts in the similar way, then it
would be difficult to use it in EM applications. Another aspect of using
exotic materials - is it hydrophilic or hydrophobic? Could you use water
with them? How easy to clean it up and handle? Sergey

At 07:00 AM 7/18/2005, you wrote:



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From: rcbaker-at-eden.infohwy.com
Date: Mon, 18 Jul 2005 21:18:00 -0500
Subject: [Microscopy] Re: Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Jul 18, 2005, at 5:48 PM, sryazant-at-ucla.edu wrote:
}
} Dear Roger
} I am not sure is tungsten carbide is similar to SiC or not. My
} experience
} with tungsten carbide basically was negative - this knife starts
} scratching
} the surface after just 50+ 0.5 um sections of standard plastic
} embedded
} tissue (no hard inclusions etc). As manufacturer explained to me it's
} because of polycrystalline nature of the material - small crystals
} just
} became loose and left the edge creating ruff surface. From this
} point of
} view, amorphous (like glass) or monocrystal (like diamond) material is
} preferable for good sections. Sapphire (hardness is 9) knifes were
} on the
} market for while without great success. From economical point of
} view,
} tungsten carbide knifes were also not so good - $100 each with
} ability to
} produce only 50+ good sections. If SiC acts in the similar way,
} then it
} would be difficult to use it in EM applications. Another aspect of
} using
} exotic materials - is it hydrophilic or hydrophobic? Could you use
} water
} with them? How easy to clean it up and handle? Sergey



Thanks everyone, I've gotten lots of helpful advice and comments, so
I'll try to sum up my conclusions.

Some background: Silicon carbide (SiC) is produced by a very old
company Washington Mills located near Niagara Falls but production is
in Illinois. Blessed folks; they sent me a sample of crystals for free.

{http://www.medibix.com/company.jsp?company_id=10001791}

It is made in tonnage quantities as clusters of glossy flat hexagonal
black crystals up to a few centimeters across, and which are actually
deep transparent blue and are crushed for abrasives and metallurgy.
The cost of this industrial crystalline material is negligible. The
Cree Corporation in NC makes also clear white SiC crystals and wafers
for a fairly high price, but only sells them already faceted into
clear diamond substitutes for jewelry. Such clear SiC crystals are
termed "moissenite".

SiC crystals are very hard and brittle and the edge breaks when you
try to sharpen them into knives using anything resembling normal gem
faceting methods. But knives can be made using the proper lapping
technique and the appropriate grades of diamond powder. Here is an
interesting link on modern diamond powder technology:

{http://www.ceramicindustry.com/CDA/ArticleInformation/features/
BNP__Features__Item/0,2710,152388,00.html}

At any rate, besides glass, only hard single crystals of diamond,
sapphire, and silicon carbide seem to be appropriate for making
ultramicrotome knives, which by their nature demand a perfectly
homogeneous material. Sapphire knives were sold at one time by
Diatome but the price was reputedly about $500 apiece and they became
dull, unlike diamond knives, so the economics was questionable.

My SiC knives become dull after a few hundred slices, probably
depending on the the hardness of the embedding plastic (glycol
methacrylate works well), but the raw materials are inexpensive and
my lapping machine only cost about $50 to build, so the labor of
making them is inherently the major cost.

Since SiC crystals are difficult to sharpen without breaking the
edge, I assume the exact same lapping techniques would also be
appropriate for sapphire, which is softer tougher and less brittle
than SiC; the latter are the very devil to sharpen without breaking.
I learned how to make them through sheer stubbornness in order to
make many serial sections with my Porter Blum MT-2 without paying
$1000+ for a diamond knife.

I can only guess at the durability of sapphire knives, but the fact
that they were once offered indicates that they might still be a
viable alternative for some applications if they were only available
at a lower cost.

My SiC crystals are epoxyed in a slot cut in the apex of a steel
holder the same size and shape as a right angle glass knife. The
clean crystals are hydrophilic (I assume the exposed surface layer at
the atomic level is silicon oxide). I clean them by wiping the edge
sideways with Teflon and don't have many wetting problems.

My conclusion is that I don't much want to go the trouble of trying
to make money patenting, licensing and selling knives, but that I
should publish my technique of making them, most likely in the Review
of Scientific Instruments, and see if the world pays attention. I
don't think the method of sharpening hard crystals to give very sharp
edges has ever been published; I can't find it in the scientific
literature. I seriously doubt it would work for diamonds without
modification.

The only possible drawback to open publication is that no one maker
could make a lot of money making them, and thus might not offer them,
assuming they prove to be a modestly useful alternative to diamonds.

I assume that maybe the Chinese could sell disposable knives for $20
or so and they might catch on for student use and low budget and
occasional use. Glass knives are clearly sharper for a few dozen
sections and diamond knives clearly last longer.

-- Roger Baker Jr.
1303 Bentwood,
Austin Tx, 78722












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From: jtwilley-at-sprynet.com
Date: Mon, 18 Jul 2005 22:24:33 -0500
Subject: [Microscopy] Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Roger,

You should be aware that recently, gem-quality silicon carbide has been under production and is
being marketed under its mineralogical name as "synthetic moissanite". This grade is significantly
more free of defects and might yield a much more durable edge. I don't know the economics of
producing a knife edge from this stock but I suspect that the material price is not prohibitively
high, given the prices of stones that are being sold as jewelry and the usual mark-up in that
field.

Sincerely,

John Twilley

rcbaker-at-eden.infohwy.com wrote:

} Thanks everyone, I've gotten lots of helpful advice and comments, so
} I'll try to sum up my conclusions.
}
} Some background: Silicon carbide (SiC) is produced by a very old
} company Washington Mills located near Niagara Falls but production is
} in Illinois. Blessed folks; they sent me a sample of crystals for free.
}
} {http://www.medibix.com/company.jsp?company_id=10001791}
}
} It is made in tonnage quantities as clusters of glossy flat hexagonal
} black crystals up to a few centimeters across, and which are actually
} deep transparent blue and are crushed for abrasives and metallurgy.
} The cost of this industrial crystalline material is negligible. The
} Cree Corporation in NC makes also clear white SiC crystals and wafers
} for a fairly high price, but only sells them already faceted into
} clear diamond substitutes for jewelry. Such clear SiC crystals are
} termed "moissenite".
}
} SiC crystals are very hard and brittle and the edge breaks when you
} try to sharpen them into knives using anything resembling normal gem
} faceting methods. But knives can be made using the proper lapping
} technique and the appropriate grades of diamond powder. Here is an
} interesting link on modern diamond powder technology:
}
} {http://www.ceramicindustry.com/CDA/ArticleInformation/features/
} BNP__Features__Item/0,2710,152388,00.html}
}
} At any rate, besides glass, only hard single crystals of diamond,
} sapphire, and silicon carbide seem to be appropriate for making
} ultramicrotome knives, which by their nature demand a perfectly
} homogeneous material. Sapphire knives were sold at one time by
} Diatome but the price was reputedly about $500 apiece and they became
} dull, unlike diamond knives, so the economics was questionable.
}
} My SiC knives become dull after a few hundred slices, probably
} depending on the the hardness of the embedding plastic (glycol
} methacrylate works well), but the raw materials are inexpensive and
} my lapping machine only cost about $50 to build, so the labor of
} making them is inherently the major cost.
}
} Since SiC crystals are difficult to sharpen without breaking the
} edge, I assume the exact same lapping techniques would also be
} appropriate for sapphire, which is softer tougher and less brittle
} than SiC; the latter are the very devil to sharpen without breaking.
} I learned how to make them through sheer stubbornness in order to
} make many serial sections with my Porter Blum MT-2 without paying
} $1000+ for a diamond knife.
}
} I can only guess at the durability of sapphire knives, but the fact
} that they were once offered indicates that they might still be a
} viable alternative for some applications if they were only available
} at a lower cost.
}
} My SiC crystals are epoxyed in a slot cut in the apex of a steel
} holder the same size and shape as a right angle glass knife. The
} clean crystals are hydrophilic (I assume the exposed surface layer at
} the atomic level is silicon oxide). I clean them by wiping the edge
} sideways with Teflon and don't have many wetting problems.
}
} My conclusion is that I don't much want to go the trouble of trying
} to make money patenting, licensing and selling knives, but that I
} should publish my technique of making them, most likely in the Review
} of Scientific Instruments, and see if the world pays attention. I
} don't think the method of sharpening hard crystals to give very sharp
} edges has ever been published; I can't find it in the scientific
} literature. I seriously doubt it would work for diamonds without
} modification.
}
} The only possible drawback to open publication is that no one maker
} could make a lot of money making them, and thus might not offer them,
} assuming they prove to be a modestly useful alternative to diamonds.
}
} I assume that maybe the Chinese could sell disposable knives for $20
} or so and they might catch on for student use and low budget and
} occasional use. Glass knives are clearly sharper for a few dozen
} sections and diamond knives clearly last longer.
}
} -- Roger Baker Jr.
} 1303 Bentwood,
} Austin Tx, 78722
}
}
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From: sousan.abolhassani-at-psi.ch
Date: Tue, 19 Jul 2005 01:34:57 -0500
Subject: [Microscopy] Re: TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: "Amit Kohn" {amit.kohn-at-materials.oxford.ac.uk

Dear Randy,

I followed this thread by curiosity as I knew
that copper is not always a good solution.
And it was good to receive your message so
that I know that there was no response that
we missed.
I think that the idea of giving a summary
of the answers to the list, is a very
respectful manner to contribute to its
usefulness.
Thanks a lot,

Sousan

TindallR-at-missouri.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} Below is a compilation of the replies I received to my recent question
} about Cu vs. Ni grids for immunolabeling. I usually don't do this
} without express permission, but most everybody posted to the list
} anyway, and I made sure to edit those that didn't. If it bothers
} anyone, let me know and I will never do it again.
}
} In summary, it appears that copper can and will react with salts in
} buffers and other solutions, although some people still have decent
} luck. This problem can be ameliorated by using coated Cu grids and only
} wetting the coated side and by shortening incubation times
}
} Also, Marc Pypaert had the interesting suggestion of just using Ni grids
} for everything, since they're not much more expensive, thereby avoiding
} the problem entirely. Any thoughts on this?
}
} Many thanks to everyone who replied! I now have a much better response
} to our clients' questions on this matter (not to mention my own).
}
} Cheers,
} Randy
}
}
} Over the years I have frequently seen cases where the samples themselves
} have reacted with the copper grid. Two examples:
}
} 1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
} precipitates were ugly and analyzed as copper sulphide. Our surmise was
} that the iron and copper had undergone ion exchange while the grid (and
} carbon film) was wet with the culture medium. When we used nickel
} grids, the precipitates were well formed and composed of iron and
} sulphur.
}
} 2) Looking at Si precipitates in Al-Si electronic bond wire. The
} specimens were prepared by embedding and microtoming, floating in DI
} water. The Si precipitates were surrounded by an ugly mess containing
} masses of copper. Again, presumed to me electrochemical reaction
} between the Al and the Cu grid, through the DI water medium, somehow
} preferentially at the Si precipitates. Again, use of Ni grids produced
} good pictures allowing us to characterize the precipitation.
}
} Having said all that, we continue to default to use copper grids!
}
} Anthony J. Garratt-Reed, M.A., D.Phil.
} MIT Room 13-1027
} 77 Massachusetts Avenue
} Cambridge, MA 02139-4307
} USA
}
}
} -------------------------------------------
}
} The main problem encountered with copper grids used for
} immunocytochemistry or immunogold labeling is the reaction of the copper
} with salts in the buffers used during labeling. This is a time-related
} reaction, and can usually be avoided by having short labeling runs and
} using grids with films on them. Gilder grids, available from major
} microscope supply vendors, are gold-coated copper grids, and are not
} that much more expensive than regular copper grids. These grids may be
} the variety used by researchers who have reported having no problems
} with their grids during their immuno runs.
} I usually use nickel grids for my work, but have used
} formvar-coated copper grids without problems for 1 day immuno runs so
} long as the film is intact and as long as I don't get clumsy and end up
} sinking my grids in my solutions (if I do sink them, I do a quick
} distilled water rinse, blot the back of the grids with filter paper
} until almost dry, then re-float them where I left off). As others have
} shared, the nickel grids are much sturdier, not too expensive, and are
} not a problem to handle as long as you use antimagnetic forceps. With
} the nickel grids, remember to correct for astigmatism in the TEM by
} using a hole in your sample on the nickel grid. This will accommodate
} for any inherent residual magnetic field in the grid itself.
}
} Edward Haller
} Lab Manager, Diagnostic Electron Microscopy Lab University of South
} Florida Pathology Department Tampa, FL 33612
}
} ------------------------------------------------------------
}
} We have had problems with Cu grids, even Formvar coated ones. I have
} precoated copper grids with Parlodion by dipping, blotting and drying
} before use, with or with out a formvar coating. THat method seems to
} protect the copper from reacting with PBS or whatever. This is not
} original to me, but I cannot recall where I read this (among many other
} things I can no longer recall). I think it might have been one of those
} smart Swiss guys.
}
} Gregory W. Erdos, Ph.D.
} Assistant Director, Biotechnology Program Scientific Director, Electron
} Microscopy P.O. Box 118525
} 217 Carr Hall
} University of Florida
} Gainesville, FL 32611
} -------------------------------------------------------------------
}
} I had a bad experience with formvar-copper grids in immunogold
} experiment many years ago when incubating primary antibody overnight and
} the rest done on the following morning. I am using Nickel grids now --
} no more problems.
}
} Ann Fook Yang
} EM Unit/ Unite EM
} Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
} 960 Carling Ave/960 Boul Carling
} Ottawa,Ontario/Ottawa, Ontario
} Canada
} K1A 0C6
} ------------------------------------------------------------------------
} ------------
}
} recently i did a long ultracentrifuge run to load some 10S particles
} onto a formvar-copper grid. the grids reacted with the phosphate buffer
} over the 4 hour spin time. not a pretty picture - i had to go back to
} my collaborator and get fresh material so we could put it onto
} formvar-nickel grids. wonderful gentleman.
}
}
} Paul R. Hazelton, PhD
} Electron Microscope Unit
} University of Manitoba
} Department of Medical Microbiology
} 531 Basic Medical Sciences Building
} 730 William Avenue
} Winnipeg, Manitoba, Canada, R3E 0W3
} ----------------------------------------------------------------
}
} We used Cu grids for many years. Normally we use formvar + carbon
} coated grids for ICC since we have a lot less loss of sections. We also
} tend to do long incubations ...usually overnight. This is time
} efficient and also lets us use highly diluted antibody so also minimize
} background due to cross-reactions from contaminants in polyclonal
} antibodies.
}
} Occasionally we would have a reaction due to copper oxidizing with
} resultant green solution. I attributed this to salts or traces of Tween
} 20 in the buffer and breaks in the coating exposing the Cu. As far as I
} can see, this is the only reason for not using Cu grids if you use
} coated grids. We have switched for the most part to using coated Ni
} grids to avoid this problem.
}
} On the other hand, occasionally we need to do a double labeling using
} uncoated grids and both sides of the sections. I would hesitate to use
} Cu for this and prefer Ni. Gold would work but is both more expensive
} and more delicate than Ni grids.
}
} Debby Sherman, Manager
} Life Science Microscopy Facility
} Purdue University
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} ------------------------------------------------------------------------
} -
}
} X-from my experience, coated copper grids will be fine for most (if not
} all) immunolabelling, providing the solutions do not wet the uncoated
} side of the grid. Uncoated copper grids always have been fine when I've
} used PBS.
}
} However, copper grids (uncoated - or the uncoated side) usually will
} react with Tris-HCl buffers and some of the high salt buffer
} formulations that I've used. Longer incubation times (eg. overnight)
} cause more reaction with the grid than shorter times (eg. 60mins) - so
} you may be able to use copper grids without problems if incubations are
} short, even with buffer formulations that might react with the grid if
} left for longer periods. It also is more difficult to prevent wetting
} of the reverse (uncoated) side of coated copper grids if incubation
} times are long, and particularly if wetting agents are used in the
} buffer formulation.
}
} When I started immunolabelling in the early 1980s - and hadn't yet heard
} about the use of nickel grids - I used uncoated copper grids (and PBS)
} for years without problems. My current lab generally uses a high salt
} Tris-HCl buffer which includes a small amount of Tween 20, so we
} routinely use nickel grids for immunolabelling. Nickel grids do have
} some disadvantages (eg. charge), but we run a multi-user facility, often
} training students or other inexperienced users, we've found nickel the
} best option to overcome potential "grid-reaction" problems. However,
} nickel grids certainly aren't essential for successful immunolabelling -
} you just need to be aware of the possible problems.
}
} Dr Deborah Stenzel
} Analytical Electron Microscopy Facility
} Queensland University of Technology
} GPO Box 2434
} Brisbane 4001
} Australia
}
} ------------------------------------------------
}
} I know (from sad experience) that Tris buffers will etch Cu grids - the
} antibody droplets turn a lovely blue and there is gunk all over the
} sections. I use Ni grids, only because I prefer Tris buffers (no real
} reason why, I guess) for IEM and my current PI cringes at buying Au
} grids.
} I know one person who does all of her labelling in phosphate buffer and
} uses Cu grids with no precipitate problems.
}
} As for the other reasons you Googled....I've heard them, but never seen
} any documentation nor had personal experience.
}
} Can't wait to hear if anyone has actual evidence for some of the other
} no-Cu reasons!
}
} Tamara Howard
} Department of Cell Biology and Physiology
} University of New Mexico - Health Sciences Center
} Albuquerque, NM 87131
} ----------------------------------------------------------------
}
} Hi Randy,
} I had problems with Cu grids when incubating sections for long periods -
} overnight for instance. The copper would react with whatever was
} available and form green-blue salts. Not unexpected. We switched to
} nickel grids for a while but they charge, gold but they are
} delicate...by this time we had shortened the incubation times used, and
} tried copper again... And had no problem using coated grids and
} incubation times of an hour or so. Grids are floated on drops of media
} so that only the coated side is wetted - I don't know if that is
} important.
}
} Dr SJ Stowe
} Facility Coordinator
} ANU Electron Microscopy Unit
} ANU CRICOS#00120C
}
}
} I only ran into the "use only Ni or Au grids" rule here, in my current
} job, and my predecessor certainly produced some superb images showing
} gold labelling of TEM sections. Blissfully unaware of this rule, I had
} been using copper grids for all EM immunolabelling. To get good
} labelling of one particular structure, which is about 40 nm diameter, I
} used uncoated thin-bar Cu grids so I could get labelling on both sides
} of the section - seemed to work just fine. I'll be interested to see
} the responses to your question.
}
} Dr. Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
}
}
}
}
}
}
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
} ==============================Original Headers==============================
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From: scott.coutts-at-med.monash.edu.au
Date: Tue, 19 Jul 2005 01:46:02 -0500
Subject: [Microscopy] Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

I need to prep some samples for SEM, but I'm not sure of the best way to
go about it.

We have some bacteria that normally grow in a type of liquid nutrient
medium as free, discrete and seperate motile cells. However, we have
come across a strain that forms into 'mats' on the bottom of the culture
vessel. It is these that we would like to examine by SEM.

The problem is that the 'mats' are not like a normal biofilm in that
they are not strongly adherant to a solid surface, and the mat is not
very robust. The mats will 'float' off the bottom of the culture vessel
if they are disturbed and they will fragment into small flakes if the
vessel is shaken or swirled. The fragments are usually a milimetre or so
square.

Of course, the 'flakes' need to be flat in order to view them properly.
I thought I might be able to pipette them, in a drop of the media
they're already in, onto nucleopore membranes and allow them to settle,
then try to remove the rest of the media and hope that they stick
through the dehydration process... but i'm not sure they will. I'm
planning on using HMDS.

I'm goig to try it just to see what happens... but has anyone prepared a
sample like this that could suggest something that is know to work well?

Cheers,

--
Scott J. Coutts
---------------------------------------------------------------
Bacterial Pathogenesis Research Group
Department of Microbiology,
Box 53, Monash University, 3800, Australia
Phone: 9905 4838 Fax: 9905 4811
---------------------------------------------------------------

==============================Original Headers==============================
8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005
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From: Rosemary.White-at-csiro.au
Date: Tue, 19 Jul 2005 01:52:08 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Scott,
Is there a specific reason for looking at these bacteria with SEM?
cheers,
Roseamry

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia


} From: scott.coutts-at-med.monash.edu.au
} Reply-To: microscopy-at-microscopy.com
} Date: Tue, 19 Jul 2005 01:47:57 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] Another SEM sample prep question
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi All,
}
} I need to prep some samples for SEM, but I'm not sure of the best way to
} go about it.
}
} We have some bacteria that normally grow in a type of liquid nutrient
} medium as free, discrete and seperate motile cells. However, we have
} come across a strain that forms into 'mats' on the bottom of the culture
} vessel. It is these that we would like to examine by SEM.
}
} The problem is that the 'mats' are not like a normal biofilm in that
} they are not strongly adherant to a solid surface, and the mat is not
} very robust. The mats will 'float' off the bottom of the culture vessel
} if they are disturbed and they will fragment into small flakes if the
} vessel is shaken or swirled. The fragments are usually a milimetre or so
} square.
}
} Of course, the 'flakes' need to be flat in order to view them properly.
} I thought I might be able to pipette them, in a drop of the media
} they're already in, onto nucleopore membranes and allow them to settle,
} then try to remove the rest of the media and hope that they stick
} through the dehydration process... but i'm not sure they will. I'm
} planning on using HMDS.
}
} I'm goig to try it just to see what happens... but has anyone prepared a
} sample like this that could suggest something that is know to work well?
}
} Cheers,
}
} --
} Scott J. Coutts
} ---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
} ---------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005
} 8, 17 -- Received: from omta04sl.mx.bigpond.com (omta04sl.mx.bigpond.com
} [144.140.93.156])
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} 8, 17 -- From: Scott Coutts {scott.coutts-at-med.monash.edu.au}
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==============================Original Headers==============================
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From: K.venner-at-ion.ucl.ac.uk
Date: Tue, 19 Jul 2005 08:24:59 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: digital ccd cameras versus digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

IMAQUE IMAGING INC CALL
571-437-6593
----- Original Message -----
X-from: {luce-at-earthtech.org}
To: {JSMIT51-at-TAMPABAY.RR.COM}
Sent: Sunday, July 17, 2005 7:05 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on
Tuesday, July 19, 2005 at 04:02:00
---------------------------------------------------------------------------

Email: K.venner-at-ion.ucl.ac.uk
Name: kerrie

Organization: Institute of Neurology, University College London UK

Title-Subject: [Filtered] MListserver: digital ccd cameras versus digital plate system for TEM

Question: Many thanks to everyone who took the time and kindly contributed to the very long debate my query sparked up. All contributions have been compiled and have enabled me to present a balanced working view of the systems currently available.

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==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Tue, 19 Jul 2005 08:40:16 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

there is a very simple way to look at bacteria in the
SEM. just puch teh bacteria/liquid through a .45
micron filter. then process the filter paper for the
SEM. this is a tried and true method.
john

--- scott.coutts-at-med.monash.edu.au wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Hi All,
}
} I need to prep some samples for SEM, but I'm not
} sure of the best way to
} go about it.
}
} We have some bacteria that normally grow in a type
} of liquid nutrient
} medium as free, discrete and seperate motile cells.
} However, we have
} come across a strain that forms into 'mats' on the
} bottom of the culture
} vessel. It is these that we would like to examine by
} SEM.
}
} The problem is that the 'mats' are not like a normal
} biofilm in that
} they are not strongly adherant to a solid surface,
} and the mat is not
} very robust. The mats will 'float' off the bottom of
} the culture vessel
} if they are disturbed and they will fragment into
} small flakes if the
} vessel is shaken or swirled. The fragments are
} usually a milimetre or so
} square.
}
} Of course, the 'flakes' need to be flat in order to
} view them properly.
} I thought I might be able to pipette them, in a drop
} of the media
} they're already in, onto nucleopore membranes and
} allow them to settle,
} then try to remove the rest of the media and hope
} that they stick
} through the dehydration process... but i'm not sure
} they will. I'm
} planning on using HMDS.
}
} I'm goig to try it just to see what happens... but
} has anyone prepared a
} sample like this that could suggest something that
} is know to work well?
}
} Cheers,
}
} --
} Scott J. Coutts
}
---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
}
---------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul
} 19 01:46:02 2005
} 8, 17 -- Received: from omta04sl.mx.bigpond.com
} (omta04sl.mx.bigpond.com [144.140.93.156])
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} ESMTP id j6J6k1Km017389
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} Jul 2005 01:46:02 -0500
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}
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} Tue, 19 Jul 2005 06:46:00 +0000
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} {42DCA1AB.5070904-at-med.monash.edu.au}
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} {scott.coutts-at-med.monash.edu.au}
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}


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From: peoshel-at-wisc.edu
Date: Tue, 19 Jul 2005 09:11:41 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Scott,

Interesting problem. The "matts" you refer to are likely to fragment
or worse if you use the usual filter-based preparation methods .
First question: do you have access to cryoSEM? If yes, this would be
the best way to do your samples. Freeze in a high-pressure freezer,
if available, or by plunging into slush nitrogen, then do the cryo
SEM. This is the method most likely to give you the unaltered
structure of the matts and the critters therein.
If you don't have access to cryoSEM, then the best way is to use
minifuge tubes or the like. Let the samples sit in the tube in fix,
then the EtOH dehydration steps, being very careful when you withdraw
the fluid to change it. Add the fluid for the next step as you
withdraw the old fluid to minimize disturbance of the matts, and
maybe add the fluid for each succeeding step down the side of the
tube.
I suggest a 2:1 1:1 1:2 EtOH:HMDS series before the 3 changes in 100% HMDS.
I've found it helpful to deposit the last HMDS + sample drops on a
sputter-coated membrane filter* stuck to a SEM stub for drying. This
further minimizes handling.
* 0.22 micron "nucleopore" type -- the ones with the nice, round
holes, not the torturous-path type of filter. Sputter coat both sides
of the filter before sticking to the stub, best, or stick to the stub
with conductive carbon tabs, then sputter coat. Then drop on the
samples in HMDS. (And yes, sputter coat for viewing in the SEM.)

Phil

} Hi All,
}
} I need to prep some samples for SEM, but I'm not sure of the best way to
} go about it.
}
} We have some bacteria that normally grow in a type of liquid nutrient
} medium as free, discrete and seperate motile cells. However, we have
} come across a strain that forms into 'mats' on the bottom of the culture
} vessel. It is these that we would like to examine by SEM.
}
} The problem is that the 'mats' are not like a normal biofilm in that
} they are not strongly adherant to a solid surface, and the mat is not
} very robust. The mats will 'float' off the bottom of the culture vessel
} if they are disturbed and they will fragment into small flakes if the
} vessel is shaken or swirled. The fragments are usually a milimetre or so
} square.
}
} Of course, the 'flakes' need to be flat in order to view them properly.
} I thought I might be able to pipette them, in a drop of the media
} they're already in, onto nucleopore membranes and allow them to settle,
} then try to remove the rest of the media and hope that they stick
} through the dehydration process... but i'm not sure they will. I'm
} planning on using HMDS.
}
} I'm goig to try it just to see what happens... but has anyone prepared a
} sample like this that could suggest something that is know to work well?
}
} Cheers,
}
} --
} Scott J. Coutts
} ---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
} ---------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 17 -- From scott.coutts-at-med.monash.edu.au Tue Jul 19 01:46:02 2005
} 8, 17 -- Received: from omta04sl.mx.bigpond.com
} (omta04sl.mx.bigpond.com [144.140.93.156])
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm

==============================Original Headers==============================
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From: tantt-at-umich.edu
Date: Tue, 19 Jul 2005 09:13:30 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: Looking T-Tool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (tantt-at-umich.edu)
from http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, July 19, 2005 at 09:03:46
---------------------------------------------------------------------------

Email: tantt-at-umich.edu
Name: Tong Tat

Organization: University of Michigan

Title-Subject: [Filtered] MListserver: Looking T-Tool

Question: Hi, does anyone know where i can purchase T-Tool Jig for TEM sample preperation?

Model No: 510A & 510B.

Its by T&T Group.

Thanks

Tongtat

---------------------------------------------------------------------------

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From: mcauliff-at-umdnj.edu
Date: Tue, 19 Jul 2005 09:32:30 -0500
Subject: [Microscopy] dye sub, ink jet etc for prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John et al.:

A few more details on what I wrote on this subject before. Lyson has
been making inks and papers for photo quality ink jet printing for some
time. Their new system is the "Daylight Darkroom". It is a dedicated 4
to 7 ink system (depending on your printer) for black and white printing
only. I think the price includes software and inks but no printer, the
system only works with certain printers. It has gotten very favorable
reviews in fine art photo magazines (May/June 2005 issue of Photo
Techniques, the review might be on the magazine's website
(phototechmag.com) or on Lyson's). Lyson will send you some sample
prints if you register at their website. I suspect that if someone sent
Lyson a digital TEM or SEM image they might make a print of that as a
sales tool. I'll bet they have no idea that there is a market for their
products in the EM field. I only wish I did more than a few prints per
year of parts of ground up cells.
Lyson is not the only firm making inks, papers and printing systems
for fine art B&W photography. Check out InkjetMall.com or MediaStreet.com.
If you want to stick with an ink jet and the manufacturer's inks and
papers the Canon i9900 is the Editor's Choice in the June 28 2005 issue
of PC magazine. The same printer is also the first choice in Photo
Techniques May/June 2005 issue. Someone on the list also liked this
printer.
If you like HP printers the PhotoSmart 8450 will take an HP 'photo
gray' cartridge set for doing black and white prints.
Disclaimer: I don't have any financial interest in any of the firms
or products I mentioned.

Geoff

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: scott.coutts-at-med.monash.edu.au
Date: Tue, 19 Jul 2005 09:53:59 -0500
Subject: [Microscopy] Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

hoffpajo-at-yahoo.com wrote:
}
} there is a very simple way to look at bacteria in the
} SEM. just puch teh bacteria/liquid through a .45
} micron filter. then process the filter paper for the
} SEM. this is a tried and true method.
} john
}

Yes, but that doesnt help me to lay mats of bacteria onto a support -
filtering them is what we normally do for the free-living cells, but if
I filter the mats, firstly there's a good chance that they;ll get stuck
in the syringe, and secondly, they won't come out flat on the filter.
THey'll be all bunched up or folded.

--
Scott J. Coutts
---------------------------------------------------------------
Bacterial Pathogenesis Research Group
Department of Microbiology,
Box 53, Monash University, 3800, Australia
Phone: 9905 4838 Fax: 9905 4811
---------------------------------------------------------------

==============================Original Headers==============================
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From: peoshel-at-wisc.edu
Date: Tue, 19 Jul 2005 10:19:15 -0500
Subject: [Microscopy] Re: Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roger,

This is interesting information. Would you be willing to write it up
for MIcroscopy Today? With illustrations and all. We are interested
in running such an article, and there would be room for the details
and so forth that you can't put in an email.

Phil
(also still not entirely retired from Tech Ed. at Microscopy Today)

} Thanks everyone, I've gotten lots of helpful advice and comments, so
} I'll try to sum up my conclusions.
}
} Some background: Silicon carbide (SiC) is produced by a very old
} company Washington Mills located near Niagara Falls but production is
} in Illinois. Blessed folks; they sent me a sample of crystals for free.
}
} {http://www.medibix.com/company.jsp?company_id=10001791}
}
} It is made in tonnage quantities as clusters of glossy flat hexagonal
} black crystals up to a few centimeters across, and which are actually
} deep transparent blue and are crushed for abrasives and metallurgy.
} The cost of this industrial crystalline material is negligible. The
} Cree Corporation in NC makes also clear white SiC crystals and wafers
} for a fairly high price, but only sells them already faceted into
} clear diamond substitutes for jewelry. Such clear SiC crystals are
} termed "moissenite".
}
} SiC crystals are very hard and brittle and the edge breaks when you
} try to sharpen them into knives using anything resembling normal gem
} faceting methods. But knives can be made using the proper lapping
} technique and the appropriate grades of diamond powder. Here is an
} interesting link on modern diamond powder technology:
}
} {http://www.ceramicindustry.com/CDA/ArticleInformation/features/
} BNP__Features__Item/0,2710,152388,00.html}
}
} At any rate, besides glass, only hard single crystals of diamond,
} sapphire, and silicon carbide seem to be appropriate for making
} ultramicrotome knives, which by their nature demand a perfectly
} homogeneous material. Sapphire knives were sold at one time by
} Diatome but the price was reputedly about $500 apiece and they became
} dull, unlike diamond knives, so the economics was questionable.
}
} My SiC knives become dull after a few hundred slices, probably
} depending on the the hardness of the embedding plastic (glycol
} methacrylate works well), but the raw materials are inexpensive and
} my lapping machine only cost about $50 to build, so the labor of
} making them is inherently the major cost.
}
} Since SiC crystals are difficult to sharpen without breaking the
} edge, I assume the exact same lapping techniques would also be
} appropriate for sapphire, which is softer tougher and less brittle
} than SiC; the latter are the very devil to sharpen without breaking.
} I learned how to make them through sheer stubbornness in order to
} make many serial sections with my Porter Blum MT-2 without paying
} $1000+ for a diamond knife.
}
} I can only guess at the durability of sapphire knives, but the fact
} that they were once offered indicates that they might still be a
} viable alternative for some applications if they were only available
} at a lower cost.
}
} My SiC crystals are epoxyed in a slot cut in the apex of a steel
} holder the same size and shape as a right angle glass knife. The
} clean crystals are hydrophilic (I assume the exposed surface layer at
} the atomic level is silicon oxide). I clean them by wiping the edge
} sideways with Teflon and don't have many wetting problems.
}
} My conclusion is that I don't much want to go the trouble of trying
} to make money patenting, licensing and selling knives, but that I
} should publish my technique of making them, most likely in the Review
} of Scientific Instruments, and see if the world pays attention. I
} don't think the method of sharpening hard crystals to give very sharp
} edges has ever been published; I can't find it in the scientific
} literature. I seriously doubt it would work for diamonds without
} modification.
}
} The only possible drawback to open publication is that no one maker
} could make a lot of money making them, and thus might not offer them,
} assuming they prove to be a modestly useful alternative to diamonds.
}
} I assume that maybe the Chinese could sell disposable knives for $20
} or so and they might catch on for student use and low budget and
} occasional use. Glass knives are clearly sharper for a few dozen
} sections and diamond knives clearly last longer.
}
} -- Roger Baker Jr.
} 1303 Bentwood,
} Austin Tx, 78722
}
}
}
}
}
}
}
}
}
}
}
}
} ==============================Original Headers==============================
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm

==============================Original Headers==============================
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From: MSHERWOOD-at-PARTNERS.ORG
Date: Tue, 19 Jul 2005 10:20:44 -0500
Subject: [Microscopy] TEM: Immunocytochemistry and Cu grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I second Sousan's reply. It saves downloading a lot of different emails! I
also found the information quite helpful.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org



-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Monday, July 18, 2005 5:22 PM
To: Sherwood, Margaret

Dear Listers,

Below is a compilation of the replies I received to my recent question
about Cu vs. Ni grids for immunolabeling. I usually don't do this
without express permission, but most everybody posted to the list
anyway, and I made sure to edit those that didn't. If it bothers
anyone, let me know and I will never do it again.

In summary, it appears that copper can and will react with salts in
buffers and other solutions, although some people still have decent
luck. This problem can be ameliorated by using coated Cu grids and only
wetting the coated side and by shortening incubation times

Also, Marc Pypaert had the interesting suggestion of just using Ni grids
for everything, since they're not much more expensive, thereby avoiding
the problem entirely. Any thoughts on this?

Many thanks to everyone who replied! I now have a much better response
to our clients' questions on this matter (not to mention my own).

Cheers,
Randy


Over the years I have frequently seen cases where the samples themselves
have reacted with the copper grid. Two examples:

1) Looking at Fe-S precipitates in magnetotactic bacteria, when the
precipitates were ugly and analyzed as copper sulphide. Our surmise was
that the iron and copper had undergone ion exchange while the grid (and
carbon film) was wet with the culture medium. When we used nickel
grids, the precipitates were well formed and composed of iron and
sulphur.

2) Looking at Si precipitates in Al-Si electronic bond wire. The
specimens were prepared by embedding and microtoming, floating in DI
water. The Si precipitates were surrounded by an ugly mess containing
masses of copper. Again, presumed to me electrochemical reaction
between the Al and the Cu grid, through the DI water medium, somehow
preferentially at the Si precipitates. Again, use of Ni grids produced
good pictures allowing us to characterize the precipitation.

Having said all that, we continue to default to use copper grids!

Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA


-------------------------------------------

The main problem encountered with copper grids used for
immunocytochemistry or immunogold labeling is the reaction of the copper
with salts in the buffers used during labeling. This is a time-related
reaction, and can usually be avoided by having short labeling runs and
using grids with films on them. Gilder grids, available from major
microscope supply vendors, are gold-coated copper grids, and are not
that much more expensive than regular copper grids. These grids may be
the variety used by researchers who have reported having no problems
with their grids during their immuno runs.
I usually use nickel grids for my work, but have used
formvar-coated copper grids without problems for 1 day immuno runs so
long as the film is intact and as long as I don't get clumsy and end up
sinking my grids in my solutions (if I do sink them, I do a quick
distilled water rinse, blot the back of the grids with filter paper
until almost dry, then re-float them where I left off). As others have
shared, the nickel grids are much sturdier, not too expensive, and are
not a problem to handle as long as you use antimagnetic forceps. With
the nickel grids, remember to correct for astigmatism in the TEM by
using a hole in your sample on the nickel grid. This will accommodate
for any inherent residual magnetic field in the grid itself.

Edward Haller
Lab Manager, Diagnostic Electron Microscopy Lab University of South
Florida Pathology Department Tampa, FL 33612

------------------------------------------------------------

We have had problems with Cu grids, even Formvar coated ones. I have
precoated copper grids with Parlodion by dipping, blotting and drying
before use, with or with out a formvar coating. THat method seems to
protect the copper from reacting with PBS or whatever. This is not
original to me, but I cannot recall where I read this (among many other
things I can no longer recall). I think it might have been one of those
smart Swiss guys.

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program Scientific Director, Electron
Microscopy P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
-------------------------------------------------------------------

I had a bad experience with formvar-copper grids in immunogold
experiment many years ago when incubating primary antibody overnight and
the rest done on the following morning. I am using Nickel grids now --
no more problems.

Ann Fook Yang
EM Unit/ Unite EM
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
960 Carling Ave/960 Boul Carling
Ottawa,Ontario/Ottawa, Ontario
Canada
K1A 0C6
------------------------------------------------------------------------
------------

recently i did a long ultracentrifuge run to load some 10S particles
onto a formvar-copper grid. the grids reacted with the phosphate buffer
over the 4 hour spin time. not a pretty picture - i had to go back to
my collaborator and get fresh material so we could put it onto
formvar-nickel grids. wonderful gentleman.


Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
----------------------------------------------------------------

We used Cu grids for many years. Normally we use formvar + carbon
coated grids for ICC since we have a lot less loss of sections. We also
tend to do long incubations ...usually overnight. This is time
efficient and also lets us use highly diluted antibody so also minimize
background due to cross-reactions from contaminants in polyclonal
antibodies.

Occasionally we would have a reaction due to copper oxidizing with
resultant green solution. I attributed this to salts or traces of Tween
20 in the buffer and breaks in the coating exposing the Cu. As far as I
can see, this is the only reason for not using Cu grids if you use
coated grids. We have switched for the most part to using coated Ni
grids to avoid this problem.

On the other hand, occasionally we need to do a double labeling using
uncoated grids and both sides of the sections. I would hesitate to use
Cu for this and prefer Ni. Gold would work but is both more expensive
and more delicate than Ni grids.

Debby Sherman, Manager
Life Science Microscopy Facility
Purdue University
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
------------------------------------------------------------------------
-

X-from my experience, coated copper grids will be fine for most (if not
all) immunolabelling, providing the solutions do not wet the uncoated
side of the grid. Uncoated copper grids always have been fine when I've
used PBS.

However, copper grids (uncoated - or the uncoated side) usually will
react with Tris-HCl buffers and some of the high salt buffer
formulations that I've used. Longer incubation times (eg. overnight)
cause more reaction with the grid than shorter times (eg. 60mins) - so
you may be able to use copper grids without problems if incubations are
short, even with buffer formulations that might react with the grid if
left for longer periods. It also is more difficult to prevent wetting
of the reverse (uncoated) side of coated copper grids if incubation
times are long, and particularly if wetting agents are used in the
buffer formulation.

When I started immunolabelling in the early 1980s - and hadn't yet heard
about the use of nickel grids - I used uncoated copper grids (and PBS)
for years without problems. My current lab generally uses a high salt
Tris-HCl buffer which includes a small amount of Tween 20, so we
routinely use nickel grids for immunolabelling. Nickel grids do have
some disadvantages (eg. charge), but we run a multi-user facility, often
training students or other inexperienced users, we've found nickel the
best option to overcome potential "grid-reaction" problems. However,
nickel grids certainly aren't essential for successful immunolabelling -
you just need to be aware of the possible problems.

Dr Deborah Stenzel
Analytical Electron Microscopy Facility
Queensland University of Technology
GPO Box 2434
Brisbane 4001
Australia

------------------------------------------------

I know (from sad experience) that Tris buffers will etch Cu grids - the
antibody droplets turn a lovely blue and there is gunk all over the
sections. I use Ni grids, only because I prefer Tris buffers (no real
reason why, I guess) for IEM and my current PI cringes at buying Au
grids.
I know one person who does all of her labelling in phosphate buffer and
uses Cu grids with no precipitate problems.

As for the other reasons you Googled....I've heard them, but never seen
any documentation nor had personal experience.

Can't wait to hear if anyone has actual evidence for some of the other
no-Cu reasons!

Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
----------------------------------------------------------------

Hi Randy,
I had problems with Cu grids when incubating sections for long periods -
overnight for instance. The copper would react with whatever was
available and form green-blue salts. Not unexpected. We switched to
nickel grids for a while but they charge, gold but they are
delicate...by this time we had shortened the incubation times used, and
tried copper again... And had no problem using coated grids and
incubation times of an hour or so. Grids are floated on drops of media
so that only the coated side is wetted - I don't know if that is
important.

Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
ANU CRICOS#00120C


I only ran into the "use only Ni or Au grids" rule here, in my current
job, and my predecessor certainly produced some superb images showing
gold labelling of TEM sections. Blissfully unaware of this rule, I had
been using copper grids for all EM immunolabelling. To get good
labelling of one particular structure, which is about 40 nm diameter, I
used uncoated thin-bar Cu grids so I could get labelling on both sides
of the section - seemed to work just fine. I'll be interested to see
the responses to your question.

Dr. Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia








Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: baskin-at-bio.umass.edu
Date: Tue, 19 Jul 2005 10:32:56 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This sounds like a job for ESEM. How much magnification do you need?
You can work with fully hydrated samples. You can't get into the
ultrastructual range of mags but excellent imaging in the classic SEM
mag range, particularly with the newer line of ESEM's.

Tobias
}
}
} hoffpajo-at-yahoo.com wrote:
} }
} } there is a very simple way to look at bacteria in the
} } SEM. just puch teh bacteria/liquid through a .45
} } micron filter. then process the filter paper for the
} } SEM. this is a tried and true method.
} } john
} }
}
} Yes, but that doesnt help me to lay mats of bacteria onto a support -
} filtering them is what we normally do for the free-living cells, but if
} I filter the mats, firstly there's a good chance that they;ll get stuck
} in the syringe, and secondly, they won't come out flat on the filter.
} THey'll be all bunched up or folded.
}
} --
} Scott J. Coutts
} ---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
} ---------------------------------------------------------------
}
}


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

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From: acarol1-at-uic.edu
Date: Tue, 19 Jul 2005 10:33:51 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, How about using a wire loop to place a mat of cells on a poly-L-lysine
coated cover slip and then running it through fix, dehydration, etc....
Maybe you don't need the loop at all. Just slide the cover slip under a
mat and lift out of the solution. I've never done a mat of cells but I've
had success with SEM of cells from suspension on coated cover slips or
pieces of Fisher Brand Plus Slides.

Andy Carol
UIC Biology
Chicago, IL

On Tue, July 19, 2005 9:54 am, scott.coutts-at-med.monash.edu.au said:
}
}
}
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}
} hoffpajo-at-yahoo.com wrote:
} } there is a very simple way to look at bacteria in the
} } SEM. just puch teh bacteria/liquid through a .45
} } micron filter. then process the filter paper for the
} } SEM. this is a tried and true method.
} } john
}
} Yes, but that doesnt help me to lay mats of bacteria onto a support -
filtering them is what we normally do for the free-living cells, but if
I filter the mats, firstly there's a good chance that they;ll get stuck
in the syringe, and secondly, they won't come out flat on the filter.
THey'll be all bunched up or folded.
}
} --
} Scott J. Coutts
} ---------------------------------------------------------------
} Bacterial Pathogenesis Research Group
} Department of Microbiology,
} Box 53, Monash University, 3800, Australia
} Phone: 9905 4838 Fax: 9905 4811
} ---------------------------------------------------------------
}
} ==============================Original
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From: henriks-at-southbaytech.com
Date: Tue, 19 Jul 2005 10:38:55 -0500
Subject: [Microscopy] Re: MicroscopyListserverviaWWW: Looking T-Tool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Tong:

You can find them at: http://www.precisiontem.com/ttools.html

If you are looking for tools for precision cross sectioning, you may
also want to visit our website and look at the the following products:

Model 590 Tripod Polisher®
Model 595 BiPod Polisher™

The Model 590 Tripod Polisher® is based on the original design by IBM
East Fishkill and is used for SEM, TEM cross sectioning. The Model 595
BiPod™ Polisher is more similar to the T-tool although it incorporates
many of the unique and critical features of the original Tripod
Polisher® as well.

Please feel free to contact me off-line for details and pricing.

Best regards-

David

tantt-at-umich.edu wrote:

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From: gcc-at-couger.com
Date: Tue, 19 Jul 2005 13:43:33 -0500
Subject: [Microscopy] Alternatives to diamond knives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Unless you can get life from the knife that is comparable to
diamond or cost comparable to glass. The cost of labor make any
thing much more expensive than glass a very hard sell when a
diamond knife cost less than a week of a lab tech time not
including the work lost while hie was messing around with knives
when he could be doing more productive work.

Gordon Couger
Stillwater, OK
www.couger.com/gcouger

rcbaker-at-eden.infohwy.com wrote:
}
} On Jul 18, 2005, at 5:48 PM, sryazant-at-ucla.edu wrote:
}
} } Dear Roger
} } I am not sure is tungsten carbide is similar to SiC or not. My
} } experience
} } with tungsten carbide basically was negative - this knife starts
} } scratching
} } the surface after just 50+ 0.5 um sections of standard plastic
} } embedded
} } tissue (no hard inclusions etc). As manufacturer explained to me it's
} } because of polycrystalline nature of the material - small crystals
} } just
} } became loose and left the edge creating ruff surface. From this
} } point of
} } view, amorphous (like glass) or monocrystal (like diamond) material is
} } preferable for good sections. Sapphire (hardness is 9) knifes were
} } on the
} } market for while without great success. From economical point of
} } view,
} } tungsten carbide knifes were also not so good - $100 each with
} } ability to
} } produce only 50+ good sections. If SiC acts in the similar way,
} } then it
} } would be difficult to use it in EM applications. Another aspect of
} } using
} } exotic materials - is it hydrophilic or hydrophobic? Could you use
} } water
} } with them? How easy to clean it up and handle? Sergey
}
}
}
}
} Thanks everyone, I've gotten lots of helpful advice and comments, so
} I'll try to sum up my conclusions.
}
} Some background: Silicon carbide (SiC) is produced by a very old
} company Washington Mills located near Niagara Falls but production is
} in Illinois. Blessed folks; they sent me a sample of crystals for free.
}
} {http://www.medibix.com/company.jsp?company_id=10001791}
}
} It is made in tonnage quantities as clusters of glossy flat hexagonal
} black crystals up to a few centimeters across, and which are actually
} deep transparent blue and are crushed for abrasives and metallurgy.
} The cost of this industrial crystalline material is negligible. The
} Cree Corporation in NC makes also clear white SiC crystals and wafers
} for a fairly high price, but only sells them already faceted into
} clear diamond substitutes for jewelry. Such clear SiC crystals are
} termed "moissenite".
}
} SiC crystals are very hard and brittle and the edge breaks when you
} try to sharpen them into knives using anything resembling normal gem
} faceting methods. But knives can be made using the proper lapping
} technique and the appropriate grades of diamond powder. Here is an
} interesting link on modern diamond powder technology:
}
} {http://www.ceramicindustry.com/CDA/ArticleInformation/features/
} BNP__Features__Item/0,2710,152388,00.html}
}
} At any rate, besides glass, only hard single crystals of diamond,
} sapphire, and silicon carbide seem to be appropriate for making
} ultramicrotome knives, which by their nature demand a perfectly
} homogeneous material. Sapphire knives were sold at one time by
} Diatome but the price was reputedly about $500 apiece and they became
} dull, unlike diamond knives, so the economics was questionable.
}
} My SiC knives become dull after a few hundred slices, probably
} depending on the the hardness of the embedding plastic (glycol
} methacrylate works well), but the raw materials are inexpensive and
} my lapping machine only cost about $50 to build, so the labor of
} making them is inherently the major cost.
}
} Since SiC crystals are difficult to sharpen without breaking the
} edge, I assume the exact same lapping techniques would also be
} appropriate for sapphire, which is softer tougher and less brittle
} than SiC; the latter are the very devil to sharpen without breaking.
} I learned how to make them through sheer stubbornness in order to
} make many serial sections with my Porter Blum MT-2 without paying
} $1000+ for a diamond knife.
}
} I can only guess at the durability of sapphire knives, but the fact
} that they were once offered indicates that they might still be a
} viable alternative for some applications if they were only available
} at a lower cost.
}
} My SiC crystals are epoxyed in a slot cut in the apex of a steel
} holder the same size and shape as a right angle glass knife. The
} clean crystals are hydrophilic (I assume the exposed surface layer at
} the atomic level is silicon oxide). I clean them by wiping the edge
} sideways with Teflon and don't have many wetting problems.
}
} My conclusion is that I don't much want to go the trouble of trying
} to make money patenting, licensing and selling knives, but that I
} should publish my technique of making them, most likely in the Review
} of Scientific Instruments, and see if the world pays attention. I
} don't think the method of sharpening hard crystals to give very sharp
} edges has ever been published; I can't find it in the scientific
} literature. I seriously doubt it would work for diamonds without
} modification.
}
} The only possible drawback to open publication is that no one maker
} could make a lot of money making them, and thus might not offer them,
} assuming they prove to be a modestly useful alternative to diamonds.
}
} I assume that maybe the Chinese could sell disposable knives for $20
} or so and they might catch on for student use and low budget and
} occasional use. Glass knives are clearly sharper for a few dozen
} sections and diamond knives clearly last longer.
}
} -- Roger Baker Jr.
} 1303 Bentwood,
} Austin Tx, 78722
}
}
}
}
}
}
}
}
}
}
}
}
} ==============================Original Headers==============================
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From: tonygr-at-MIT.EDU
Date: Tue, 19 Jul 2005 14:03:32 -0500
Subject: [Microscopy] Position for microscopist at MIT (for your review)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Center for Materials Science and Engineering at the Massachusetts
Institute of Technology has an immediate vacancy for a research specialist
in electron microscopy. A description of the vacancy, together with MIT's
employment policies, benefits, and other information, with links for
on-line application is available at the following URL:
http://sh.webhire.com/servlet/av/jd?ai=631&ji=1620589&sn=I
or applications may be forwarded by electronic or paper mail to Ms.
Jennifer Crockett, CMSE Assistant Director, Room # 13-2106, Massachusetts
Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307,
e-mail crockett-at-mit.edu.

The application review process will be open until a qualified candidate has
been identified. Questions about this position may be addressed to the
undersigned.

Tony Garratt-Reed



***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


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From: sryazant-at-ucla.edu
Date: Tue, 19 Jul 2005 16:01:12 -0500
Subject: [Microscopy] Re: dye sub, ink jet etc for prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I used to work with Lyson "grey" inks and paper. It was previous
generation, not "Daylight Darkroom" line. The quality of images was (yes,
was) outstanding - no comparison to laser or dye-sub. The problem appeared
to happens month later - the images faded very quickly: in month they lost
about 50% of tone. I was trying different type of paper - no luck. Right
now I am trying "grey" inks from different vendor, so I'll see. As far as
I understand from numerous conversation with people from graphic design and
digital photography area, it's practically impossible to protect ink-jet
images from fading if stored not in album with protective cover. Another
thing: glossy paper always bad in terms of fading. Matte paper is the
best. Pigment inks are better than organic, but tends to clog the printer's
head. After HP patent on ink-jet technology expired, Epson jumped into the
market with few serious enhancements of the HP technology. I think they
used piezo-element to disperse the inks and probably some other tricks. I
am very happy with Epson 890 model. It was quite expensive a few years
ago. You could get it for 10$ at eBay now. It has 6 inks and quality of
color (with Epson cartridges) and B&W prints just outstanding. It's quite
slow and as I mentioned above B&W images with Lyson inks tends to fade
quick. No interest in Epson, just happy user. Sergey

At 07:34 AM 7/19/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: K.venner-at-ion.ucl.ac.uk
Date: Tue, 19 Jul 2005 16:45:01 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: sem prep of floating cell mats

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Tuesday, July 19, 2005 at 10:11:07
---------------------------------------------------------------------------

Email: K.venner-at-ion.ucl.ac.uk
Name: kerrie

Organization: ION, UCL UK

Title-Subject: [Filtered] sem prep of floating cell mats

Question: Never done this for sample prep, but it's a mind experiment: how about putting the cells and their media into a vessel like a bath with a plug at the bottom. Submerge the millipores and when they are sitting on the bottom, let the media out via the plug hole. I would say that the cell mats would settle gently with few creases. Sandwich lightly in a cut-down Swinnex holder, process and cpd intact. Just an idea from the old way I learned to coat grids with formvar.

---------------------------------------------------------------------------

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From: peoshel-at-wisc.edu
Date: Wed, 20 Jul 2005 08:20:17 -0500
Subject: [Microscopy] Re: Another SEM sample prep question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Phil,

Would not critical point drying them in one of our microporous specimen
capsules be the best approach? See URL
http://www.2spi.com/catalog/instruments/microporous.shtml

Chuck
----- Original Message -----
X-from: {peoshel-at-wisc.edu}
To: {cgarber-at-2spi.com}
Sent: Tuesday, July 19, 2005 10:13 AM

Chuck,

Well, he wants to try HMDS, and that works very well for bacteria.
CPD is extra handling, and he's dealing with fragile biofilm mats, so
that's another reason. It can work, but the less handling the better.
I also don't use the porous capsules anymore. Too much trouble with
staticy specimens after drying, and too many staticy little white
bits of capsule coming off and getting on the specimens.

Phil

} Hello Phil,
}
} Would not critical point drying them in one of our microporous specimen
} capsules be the best approach? See URL
} http://www.2spi.com/catalog/instruments/microporous.shtml
}
} Chuck

} } Scott,
} }
} } Interesting problem. The "matts" you refer to are likely to fragment
} } or worse if you use the usual filter-based preparation methods .
} } First question: do you have access to cryoSEM? If yes, this would be
} } the best way to do your samples. Freeze in a high-pressure freezer,
} } if available, or by plunging into slush nitrogen, then do the cryo
} } SEM. This is the method most likely to give you the unaltered
} } structure of the matts and the critters therein.
} } If you don't have access to cryoSEM, then the best way is to use
} } minifuge tubes or the like. Let the samples sit in the tube in fix,
} } then the EtOH dehydration steps, being very careful when you withdraw
} } the fluid to change it. Add the fluid for the next step as you
} } withdraw the old fluid to minimize disturbance of the matts, and
} } maybe add the fluid for each succeeding step down the side of the
} } tube.
} } I suggest a 2:1 1:1 1:2 EtOH:HMDS series before the 3 changes in 100%
} } HMDS.
} } I've found it helpful to deposit the last HMDS + sample drops on a
} } sputter-coated membrane filter* stuck to a SEM stub for drying. This
} } further minimizes handling.
} } * 0.22 micron "nucleopore" type -- the ones with the nice, round
} } holes, not the torturous-path type of filter. Sputter coat both sides
} } of the filter before sticking to the stub, best, or stick to the stub
} } with conductive carbon tabs, then sputter coat. Then drop on the
} } samples in HMDS. (And yes, sputter coat for viewing in the SEM.)
} }
} } Phil
} }
} } } Hi All,
} } }
} } } I need to prep some samples for SEM, but I'm not sure of the best way to
} } } go about it.
} } }
} } } We have some bacteria that normally grow in a type of liquid nutrient
} } } medium as free, discrete and seperate motile cells. However, we have
} } } come across a strain that forms into 'mats' on the bottom of the culture
} } } vessel. It is these that we would like to examine by SEM.
} } }
} } } The problem is that the 'mats' are not like a normal biofilm in that
} } } they are not strongly adherant to a solid surface, and the mat is not
} } } very robust. The mats will 'float' off the bottom of the culture vessel
} } } if they are disturbed and they will fragment into small flakes if the
} } } vessel is shaken or swirled. The fragments are usually a milimetre or so
} } } square.
} } }
} } } Of course, the 'flakes' need to be flat in order to view them properly.
} } } I thought I might be able to pipette them, in a drop of the media
} } } they're already in, onto nucleopore membranes and allow them to settle,
} } } then try to remove the rest of the media and hope that they stick
} } } through the dehydration process... but i'm not sure they will. I'm
} } } planning on using HMDS.
} } }
} } } I'm goig to try it just to see what happens... but has anyone prepared a
} } } sample like this that could suggest something that is know to work well?
} } }
} } } Cheers,
} } }
} } } --
} } } Scott J. Coutts
} } } ---------------------------------------------------------------
} } } Bacterial Pathogenesis Research Group
} } } Department of Microbiology,
} } } Box 53, Monash University, 3800, Australia
} } } Phone: 9905 4838 Fax: 9905 4811
} } } ---------------------------------------------------------------
} } }
} } } ==============================Original
} } } Headers==============================
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} }
} } --
} } Philip Oshel
} } Supervisor, BBPIC microscopy facility
} } Department of Animal Sciences
} } University of Wisconsin
} } 1675 Observatory Drive
} } Madison, WI 53706
} } voice: (608) 263-4162
} } fax: (608) 262-5157 (dept. fax)
} } http://www.ansci.wisc.edu/microscopy.htm
} }
} } ==============================Original
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From: RossLM-at-missouri.edu
Date: Wed, 20 Jul 2005 08:35:36 -0500
Subject: [Microscopy] Re: HR images with Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ezer,

Do you want to use an imaging program or by x-ray microanalysis? If
imaging, one shareware program you can use is Image J from NIH. Do a
search for image j and it will take you to the download site. For
simple measurements all you need to do is select the straight line
tool, draw a line over the scale bar, and then set scale from a pull
down menu. After that, with the same tool to draw a line over the
distance you want to measure, keeping the mouse button down, and in
the information field will be the line distance.

If by microanalysis, there is a program called GMR Film in the MSA
library that you can download. It is based on K-ratio measurements so
you need standards, and the film has to be smaller than the
interaction volume. It is an old DOS program but works with XP, and
I'm sure there are newer programs pout there too.

If you need any other help, feel free to contact me off-line,
Lou Ross


} ------------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


--
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=2227
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu

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From: westlab-at-linkline.com
Date: Wed, 20 Jul 2005 17:49:14 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: Tegal Plasmod etcher manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (westlab-at-linkline.com) from http://www.microscopy.com/MLFormMail.html on Wednesday, July 20, 2005 at 16:12:07
---------------------------------------------------------------------------

Email: westlab-at-linkline.com
Name: Mike Maladzhikyan

Organization: Western Analytical Lab

Title-Subject: [Filtered] Tegal Plasmod etcher manual

Question: Hi,

I am wondering if anyone has the Tegal Plasmod manual available? We acquired this etcher on the used market and it did not come with a manual. Please email me. I will be happy to pay you for your time and the copying costs. Thanks.

---------------------------------------------------------------------------

==============================Original Headers==============================
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7, 12 -- Subject: MicroscopyListserverviaWWW: Tegal Plasmod etcher manual
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From: Joseph_Oparowski-at-bose.com
Date: Thu, 21 Jul 2005 05:56:38 -0500
Subject: [Microscopy] Recall: MicroscopyListserverviaWWW: Tegal Plasmod etcher manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The sender would like to recall the message, "[Microscopy] MicroscopyListserverviaWWW: Tegal Plasmod etcher manual".


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From: Diane.Ciaburri-at-gd-ais.com
Date: Thu, 21 Jul 2005 10:36:39 -0500
Subject: [Microscopy] MicroscopyListserverviaWWW: Tegal Plasmod etcher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've got one. It's only 8 pages long so it won't take long to scan it
in. I'll send it shortly.

Diane Ciaburri

-----Original Message-----
X-from: westlab-at-linkline.com [mailto:westlab-at-linkline.com]
Sent: Wednesday, July 20, 2005 6:49 PM
To: Ciaburri, Diane A

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (westlab-at-linkline.com) from
http://www.microscopy.com/MLFormMail.html on Wednesday, July 20, 2005 at
16:12:07
------------------------------------------------------------------------
---

Email: westlab-at-linkline.com
Name: Mike Maladzhikyan

Organization: Western Analytical Lab

Title-Subject: [Filtered] Tegal Plasmod etcher manual

Question: Hi,

I am wondering if anyone has the Tegal Plasmod manual available? We
acquired this etcher on the used market and it did not come with a
manual. Please email me. I will be happy to pay you for your time and
the copying costs. Thanks.

------------------------------------------------------------------------
---

==============================Original
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From: lcgould-at-med.cornell.edu
Date: Thu, 21 Jul 2005 13:46:10 -0500
Subject: [Microscopy] Re: gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,
Here's the problem I hope someone out there can help me with....
I have a set of samples that I need to embed for TEM. I've tried
previous sets, with poor results. The samples are cells plated on
collagen gels or on fibronectin in those 35mm cover-slip bottomed
dishes (available commercially). The cells have been pfa fixed,
antibody labelled with peroidase-DAB as the chromogen, then fixed in
4% buffered glutaradehyde. The problem I've had is that in the end,
the blocks have come out gummy rather than hard, so that they cannot
be sectioned.
I took some plain dishes and tested them out with both the Spurr's
and LX-112. The blocks were fine, so the problem is not an
interaction between the plastic of the dish and the resin (I embed by
over-filling the BEEM capsule and inverting the sample over the
meniscus of the resin).
I have extended the dehydration steps to 2 changes of 15 minutes each
at each of the ethanol concentrations, used a new bottle of 100% at
the end, and left the samples to infiltrate with the resin before
placing them in the oven.
This week I tried 2 sample dishes. One seems like regions of the
block face will cut well, but there may not be enough material to cut
away a piece to re-embed so that I can cut both en face and cross
sections. The second dish is gummy...the dish pulled off leaving a
rough surface (vs snapping off and leaving a clean , smooth face).
I can't use PO at the end of the dehydration because it will dissolve
the dish, same for acetone as a dehydrant. Aside from moving to
Phoenix (for both lower humidity and anonymity), I am at a loss.
Any ideas?
Thanks,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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1, 22 -- Date: Thu, 21 Jul 2005 14:46:00 -0400
1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
1, 22 -- Subject: Re: gummy blocks
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From: hbarwood-at-troy.edu
Date: Thu, 21 Jul 2005 14:02:29 -0500
Subject: [Microscopy] Mechanical repair help?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have an old Simplex microscope (actually Leitz,) with one of the massive
stands used for this type of measuring microscope. The micrometer height
adjustment went out on me years ago and I've just been using the threaded
height adjustor on the column to focus the scope. Now, I'm at a point where
I really need the fine adjustment. Is there anyone out there who can
disassemble the focusing rack that holds the scope and replace the small
ball bearings so it will function again? If anyone can help, please let me
know. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu


==============================Original Headers==============================
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From: Winston.Wiggins-at-cshs.org
Date: Thu, 21 Jul 2005 14:33:59 -0500
Subject: [Microscopy] Re: gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Leona,
What time and temperature did you use to cure the blocks? You can try using
a lower temperature for a longer time to polymerize the blocks. That's
worked for me when I've had gummy bears ~, ah, blocks, that is!
Winston

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, E.M. Pathology Asst.
CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
Electron Microscopy, LLSPT, A823-A828
8700 Beverly Blvd.
Los Angeles, CA 90048
310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
Winston.Wiggins-at-CSHS.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


-----Original Message-----
X-from: lcgould-at-med.cornell.edu [mailto:lcgould-at-med.cornell.edu]
Sent: Thursday, July 21, 2005 11:51 AM
To: Winston.Wiggins-at-CSHS.org

Hi All,
Here's the problem I hope someone out there can help me with....
I have a set of samples that I need to embed for TEM. I've tried
previous sets, with poor results. The samples are cells plated on
collagen gels or on fibronectin in those 35mm cover-slip bottomed
dishes (available commercially). The cells have been pfa fixed,
antibody labelled with peroidase-DAB as the chromogen, then fixed in
4% buffered glutaradehyde. The problem I've had is that in the end,
the blocks have come out gummy rather than hard, so that they cannot
be sectioned.
I took some plain dishes and tested them out with both the Spurr's
and LX-112. The blocks were fine, so the problem is not an
interaction between the plastic of the dish and the resin (I embed by
over-filling the BEEM capsule and inverting the sample over the
meniscus of the resin).
I have extended the dehydration steps to 2 changes of 15 minutes each
at each of the ethanol concentrations, used a new bottle of 100% at
the end, and left the samples to infiltrate with the resin before
placing them in the oven.
This week I tried 2 sample dishes. One seems like regions of the
block face will cut well, but there may not be enough material to cut
away a piece to re-embed so that I can cut both en face and cross
sections. The second dish is gummy...the dish pulled off leaving a
rough surface (vs snapping off and leaving a clean , smooth face).
I can't use PO at the end of the dehydration because it will dissolve
the dish, same for acetone as a dehydrant. Aside from moving to
Phoenix (for both lower humidity and anonymity), I am at a loss.
Any ideas?
Thanks,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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1, 22 -- Date: Thu, 21 Jul 2005 14:46:00 -0400
1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
1, 22 -- Subject: Re: gummy blocks
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From: Wade.McFaddin-at-NextekInc.com
Date: Thu, 21 Jul 2005 14:40:11 -0500
Subject: [Microscopy] Mechanical repair help?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Henry,

We have used Vashaw Scientific in Norcross, Ga. for service and repair on
our Leica/Leitz microscopes. The service engineer's name is David Benjamin,
phone number is 770-447-5632 and email address is "dbenjamin-at-vashaw.com"

I have no vested interest in Vashaw Scientific, but have enjoyed excellent
sales support and service from them for over 20 years.

Good luck,
Wade McFaddin
Nextek Inc.
Madison, Al.

-----Original Message-----
X-from: hbarwood-at-troy.edu [mailto:hbarwood-at-troy.edu]
Sent: Thursday, July 21, 2005 2:03 PM
To: wade.mcfaddin-at-nextekinc.com

I have an old Simplex microscope (actually Leitz,) with one of the massive
stands used for this type of measuring microscope. The micrometer height
adjustment went out on me years ago and I've just been using the threaded
height adjustor on the column to focus the scope. Now, I'm at a point where
I really need the fine adjustment. Is there anyone out there who can
disassemble the focusing rack that holds the scope and replace the small
ball bearings so it will function again? If anyone can help, please let me
know. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu


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12, 11 -- From Wade.McFaddin-at-NextekInc.com Thu Jul 21 14:40:11 2005
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From: mcauliff-at-umdnj.edu
Date: Thu, 21 Jul 2005 14:44:06 -0500
Subject: [Microscopy] gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Lee:

I have found that at least one Spurr component (DER or ERL, maybe
both) does react with plastic culture dishes over a few hours so that
may be the cause. I tested the components individually, not the final
mixture. I don't know about LX112. You can skip intermediate slovents
like PO with Epon substitutes, Epon is miscible with ethanols even with
some water remaining. When I did cultures I went from abs. EtOH to an
EtOH:Epon mix (2:1 first, then 1:2 each for an hour) with agitation
(slowly on a shaker table or just tilting the dish by hand every so
often), then several changes of pure Epon several hours each, at least
one under vacuum.
You should also check your accelerator, replace if more than 6
months old. Also, I don't use DMP-30, I use BDMA instead. I don't see
anything in your protocol that should cause problems. What happens if
you allow the epoxy to polymerize by itself, no contact with culture dish?

Geoff

lcgould-at-med.cornell.edu wrote:

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From: thoward-at-unm.edu
Date: Thu, 21 Jul 2005 14:50:25 -0500
Subject: [Microscopy] gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've had this problem with cultures grown on Matrigel- as well as plain
old collagen-coated plates. These substrates are very hydrated, so
extending your dehydration times and doing a few extra changes of
absolute, dry ethanol (or acetone) at the end should take care of the
gummy-block syndrome.

Good luck!

Tamara

On Thu, 21 Jul 2005 lcgould-at-med.cornell.edu wrote:

}
}
}
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} Hi All,
} Here's the problem I hope someone out there can help me with....
} I have a set of samples that I need to embed for TEM. I've tried
} previous sets, with poor results. The samples are cells plated on
} collagen gels or on fibronectin in those 35mm cover-slip bottomed
} dishes (available commercially). The cells have been pfa fixed,
} antibody labelled with peroidase-DAB as the chromogen, then fixed in
} 4% buffered glutaradehyde. The problem I've had is that in the end,
} the blocks have come out gummy rather than hard, so that they cannot
} be sectioned.
} I took some plain dishes and tested them out with both the Spurr's
} and LX-112. The blocks were fine, so the problem is not an
} interaction between the plastic of the dish and the resin (I embed by
} over-filling the BEEM capsule and inverting the sample over the
} meniscus of the resin).
} I have extended the dehydration steps to 2 changes of 15 minutes each
} at each of the ethanol concentrations, used a new bottle of 100% at
} the end, and left the samples to infiltrate with the resin before
} placing them in the oven.
} This week I tried 2 sample dishes. One seems like regions of the
} block face will cut well, but there may not be enough material to cut
} away a piece to re-embed so that I can cut both en face and cross
} sections. The second dish is gummy...the dish pulled off leaving a
} rough surface (vs snapping off and leaving a clean , smooth face).
} I can't use PO at the end of the dehydration because it will dissolve
} the dish, same for acetone as a dehydrant. Aside from moving to
} Phoenix (for both lower humidity and anonymity), I am at a loss.
} Any ideas?
} Thanks,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy & Histology Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
} ==============================Original Headers==============================
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} 1, 22 -- Date: Thu, 21 Jul 2005 14:46:00 -0400
} 1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
} 1, 22 -- Subject: Re: gummy blocks
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}

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|

==============================Original Headers==============================
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From: redhair-at-stanford.edu
Date: Thu, 21 Jul 2005 16:03:40 -0500
Subject: [Microscopy] gummy blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Lee .Can you remove the coverslip from the dish after fixation and
process it alone? That way you could embed the whole coverslip in a Chang
mold, remove the glass using HF and punch out cells from the wafer. You
could also re-embed chips to cross-section. You would avoid the dish problem.
I have a feeling that your problems could be due to solutions getting
trapped under the coverslip (I am not familiar with this type of dish) or
sometimes the coating on the coverslip causes problems with removing the
inverted Beem capsules. The whole coating comes off leaving the rough
surface you mention. You might also want to check your resin and make sure
it is freshly made. Good luck, JoAnn
01:49 PM 7/21/2005 -0500, you wrote:



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Department of Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856


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From: mpease-at-jhmi.edu
Date: Thu, 21 Jul 2005 16:08:21 -0500
Subject: [Microscopy] Re: gummy blocks (Out of the office/lab)

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the lab from Friday, July 22 through Friday, August 5, 2005.

If you need to speak with someone regarding the Microscopy and Imaging Core Facility, please contact our microscopy specialist, Rhonda Grebe, at 5-2597 for assistance; otherwise I will get back to you on Monday, August 8.

Mary Ellen


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From: psconnel-at-sas.upenn.edu
Date: Thu, 21 Jul 2005 16:46:32 -0500
Subject: [Microscopy] gummy blocks

Contents Retrieved from Microscopy Listserver Archives
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Lee,
Those small well dishes do not do so well when trying to exchange fluids. I
have a few suggestions and you may wish to try one or all.

1. I use a rotating table so that the fluids are swirling during the time that
they are sitting.
2. The dehydration times for single cells seems fine but adding the gel with
cells on top is equivalent to thin blocks so that I would suggest doubling the
time in 100% EtOH.
3. For many years I have been using HPMA (Hydroxypropyl Methacrylate)in place
of Propylene Oxide both after the 100% EtOH and in a 1:1 and a 2:1::Epon:HPMA
mixture before going into 100% Epon at least 3 times before embedding.

I do not know the reference for using HPMA. I was instructed to use it in
plastic Tissue Culture dishes when I first learned embedding back in 1971.
(Thanks to whomever the first one was who tried it!)

I agree that the LX-112 works great as the substitute for the original epon
which does not melt the plastics that the dishes are made from.

Pat Connelly
Univ. of Pennsylvania
psconnel-at-sas.upenn.edu

} ----------------------------------------------------------------------------

} Here's the problem I hope someone out there can help me with....
} I have a set of samples that I need to embed for TEM. I've tried
} previous sets, with poor results. The samples are cells plated on
} collagen gels or on fibronectin in those 35mm cover-slip bottomed
} dishes (available commercially). The cells have been pfa fixed,
} antibody labelled with peroidase-DAB as the chromogen, then fixed in
} 4% buffered glutaradehyde. The problem I've had is that in the end,
} the blocks have come out gummy rather than hard, so that they cannot
} be sectioned.
} I took some plain dishes and tested them out with both the Spurr's
} and LX-112. The blocks were fine, so the problem is not an
} interaction between the plastic of the dish and the resin (I embed by
} over-filling the BEEM capsule and inverting the sample over the
} meniscus of the resin).
} I have extended the dehydration steps to 2 changes of 15 minutes each
} at each of the ethanol concentrations, used a new bottle of 100% at
} the end, and left the samples to infiltrate with the resin before
} placing them in the oven.
} This week I tried 2 sample dishes. One seems like regions of the
} block face will cut well, but there may not be enough material to cut
} away a piece to re-embed so that I can cut both en face and cross
} sections. The second dish is gummy...the dish pulled off leaving a
} rough surface (vs snapping off and leaving a clean , smooth face).
} I can't use PO at the end of the dehydration because it will dissolve
} the dish, same for acetone as a dehydrant. Aside from moving to
} Phoenix (for both lower humidity and anonymity), I am at a loss.
} Thanks,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy & Histology Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College of Cornell University

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From: r.sims-at-auckland.ac.nz
Date: Thu, 21 Jul 2005 17:10:42 -0500
Subject: [Microscopy] Sticky Stage on 840A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

The stage on my JSM 840A is pushed by linkage from the micrometer
knob in one X direction, and pulled back by a spring in the other X
direction.

It has become a bit sticky, so that the spring pulls it back only slowly. Some
users find this disconcerting.

Should I clean it or lubricate it?

If the latter, with what?

Diff pump oil?


cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


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From: jehrman-at-mta.ca
Date: Thu, 21 Jul 2005 19:17:21 -0500
Subject: [Microscopy] Re: Sticky Stage on 840A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ritchie,
I find that if you take off the micrometer or undo the gears inside and take
the shaft right out of the feedthrough, you can clean the stiff black stuff
off the shaft with lab alcohol. Take out the o-ring and clean it and its
groove and then lubricate the o-ring lightly with Apiazon L high vacuum
grease. Check that the spring isn't gummed up, as well. If you cannot get
the shaft out, run it to one end and clean all that is exposed and then run
it to the other end and clean the rest. If you cannot get out the o-ring,
you can put a bit of high vacuum grease on the clean shaft and then run it
into the o-ring hole.
----- Original Message -----
X-from: {r.sims-at-auckland.ac.nz}
To: {mager-at-interchange.ubc.ca}
Sent: Thursday, July 21, 2005 3:14 PM

Hi Ritchie,

Had the same problem a while back on our 5600. Our serviceman told me to
use a bit of RP oil. I
took the micrometer completely off and ran it through it's entire
travel, adding a small amount (just a
few drops total) as I went. It didn't really help at first, but then it
must have coated the sticky part, because
it became silky smooth again. It appears, though, that once they start
doing this, they need to have this
done every year or so. Mine is starting to get a little stiff again.

Hope this helps,

Jim

r.sims-at-auckland.ac.nz wrote:

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--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf



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From: garyeaston-at-scannerscorp.com
Date: Thu, 21 Jul 2005 19:47:04 -0500
Subject: [Microscopy] Re: Sticky Stage on 840A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ritchie,
Your problem is more than likely caused by a sticky universal "slip
joint" that connects the external X micrometer with the X stage slide,
not a sticky X bearing slide. You can check this by moving the
universal itself in a back & forth motion. I would guess that the "Y"
universal moves freely and the X universal is sticky. Or, I have seen
those anti-backlash springs get weak after 20 years or so. And ,
lubricating your stage with anything, even dry graphite is not a good
thing to do with regards to your vacuum system (cleanliness, ultimate
vacuum, sample contamination, etc).

Gary M. Easton
Scanners Corporation
3rd Party SEM Service

r.sims-at-auckland.ac.nz wrote:

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From: qxing-at-uno.edu
Date: Thu, 21 Jul 2005 22:02:30 -0500
Subject: [Microscopy] AskAMicroscopist: How to index high order Kikuchi lines of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(qxing-at-uno.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 21, 2005 at 12:25:38
---------------------------------------------------------------------------

Email: qxing-at-uno.edu
Name: Qingfeng Xing

Organization: University of New Orleans

Education: Graduate College

Location: New Orleans LA 70148

Question: How to index high order Kikuchi lines of silicon?

Clear high order Kikuchi lines can be obtained by CBED at an orientation far from low-index zones. I found that it's difficult to index them even knowing the position of the direct beam on the Kickuchi map, as there are so many lines. Is there any procedure to do that?

Thank you very much.

---------------------------------------------------------------------------

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9, 13 -- silicon?
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From: uti-at-direcpc.com
Date: Fri, 22 Jul 2005 06:21:11 -0500
Subject: [Microscopy] Nikon D-70 for optical microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Group,

We plan to make a modification for our optical motorized
microscope and consider an option to go with Nikon D-70.
If any one could share experience to adopt such camera
for science, would you please share with me?
There is any software, which could control the camera?
Any preferences? Any other sources of information?
There is another cameras/software suitable for such upgrade?

Thank you very much,
Vlad



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From: emlabservices-at-cox.net
Date: Fri, 22 Jul 2005 06:29:20 -0500
Subject: [Microscopy] SEM: 840A Sticky Stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

To add oil to your stage is nothing more than accelerated back-streaming. If
the stage is sticking or "jumpy" when translating the sample, the best
approach is to minimally disassemble, thoroughly clean, re-surface ball
bearing races where necessary, inspect for worn parts, and reassemble as it
was.

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St.
Topeka, Kansas 66617-1780
785.246.1232 voice
785.246.0168 fax
www.emlabservices.com



==============================Original Headers==============================
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From: j.bilde-at-risoe.dk
Date: Fri, 22 Jul 2005 06:49:40 -0500
Subject: [Microscopy] AskAMicroscopist: How to index high order Kikuchi lines of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Qingfeng,

You can get various programs to simulate the HOLZ lines. You can also use the on-line resources developed by Stadelmann at Lausanne. The webaddress is:

http://cimesg1.epfl.ch/CIOL/ems.html

Best regards,
Jorgen.
{:::} --- {:::} --- {:::} --- {:::} --- {:::} --- {:::}

Joergen B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 4677 5802 (direct)
phone: +45 4677 4677 (switchboard)
fax: +45 4677 5758
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm



-----Original Message-----
X-from: qxing-at-uno.edu [mailto:qxing-at-uno.edu]
Sent: 22. juli 2005 05:04
To: j.bilde-at-risoe.dk

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by
(qxing-at-uno.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, July 21, 2005 at 12:25:38
---------------------------------------------------------------------------

Email: qxing-at-uno.edu
Name: Qingfeng Xing

Organization: University of New Orleans

Education: Graduate College

Location: New Orleans LA 70148

Question: How to index high order Kikuchi lines of silicon?

Clear high order Kikuchi lines can be obtained by CBED at an orientation far from low-index zones. I found that it's difficult to index them even knowing the position of the direct beam on the Kickuchi map, as there are so many lines. Is there any procedure to do that?

Thank you very much.

---------------------------------------------------------------------------

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From: clei-at-uiuc.edu
Date: Fri, 22 Jul 2005 08:41:49 -0500
Subject: [Microscopy] Re: AskAMicroscopist: How to

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ask Prof. Zhou Weilie at UNO. He knows the answer.


---- Original message ----
} Date: Thu, 21 Jul 2005 22:04:03 -0500
} From: qxing-at-uno.edu
} Subject: [Microscopy] AskAMicroscopist: How to index high
order Kikuchi lines of
} To: clei-at-uiuc.edu
}
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From: hoffpajo-at-yahoo.com
Date: Fri, 22 Jul 2005 11:51:03 -0500
Subject: [Microscopy] gummy blocks

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i haven't had the time to follow the thread so if
someone has suggested this then just ignore. you can
buy polyproplene dishes that work just as well so you
can use PO, there are also polypropylene cover slips
as well as slide, i thing the use of PO will solve
your gummy blocks
john

--- lcgould-at-med.cornell.edu wrote:

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} Hi All,
} Here's the problem I hope someone out there can
} help me with....
} I have a set of samples that I need to embed for
} TEM. I've tried
} previous sets, with poor results. The samples are
} cells plated on
} collagen gels or on fibronectin in those 35mm
} cover-slip bottomed
} dishes (available commercially). The cells have been
} pfa fixed,
} antibody labelled with peroidase-DAB as the
} chromogen, then fixed in
} 4% buffered glutaradehyde. The problem I've had is
} that in the end,
} the blocks have come out gummy rather than hard, so
} that they cannot
} be sectioned.
} I took some plain dishes and tested them out with
} both the Spurr's
} and LX-112. The blocks were fine, so the problem is
} not an
} interaction between the plastic of the dish and the
} resin (I embed by
} over-filling the BEEM capsule and inverting the
} sample over the
} meniscus of the resin).
} I have extended the dehydration steps to 2 changes
} of 15 minutes each
} at each of the ethanol concentrations, used a new
} bottle of 100% at
} the end, and left the samples to infiltrate with the
} resin before
} placing them in the oven.
} This week I tried 2 sample dishes. One seems like
} regions of the
} block face will cut well, but there may not be
} enough material to cut
} away a piece to re-embed so that I can cut both en
} face and cross
} sections. The second dish is gummy...the dish pulled
} off leaving a
} rough surface (vs snapping off and leaving a clean ,
} smooth face).
} I can't use PO at the end of the dehydration because
} it will dissolve
} the dish, same for acetone as a dehydrant. Aside
} from moving to
} Phoenix (for both lower humidity and anonymity), I
} am at a loss.
} Any ideas?
} Thanks,
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy & Histology Core
} Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
} ==============================Original
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} 13:46:10 2005
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__________________________________________________
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From: walck-at-southbaytech.com
Date: Fri, 22 Jul 2005 12:07:28 -0500
Subject: [Microscopy] AskAMicroscopist: How to index high order Kikuchi

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I only saw one response to your question, so I thought that I would jump
in and try to help. As was pointed out in the posting from Jorgen
Bilde, you can get a program to help you, but you can also do it
manually with some effort.

If you know the orientation of the beam, then you can figure out the
range of indices that match or closely matches the g "dot" =1 or 2
condition (where B is the beam direction). You have to know the angular
half angle to calculate the range of indices to use. Once you find the
indices, you sort them to find the order from the exact Bragg condition
(center of disk).

In Mike Kersker's (JEOL, USA) Ph.D. dissertation from , there is a nice
demonstration on how to do this with an ordered alloy phase.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: qxing-at-uno.edu [mailto:qxing-at-uno.edu]
Sent: Thursday, July 21, 2005 8:07 PM
To: Walck-at-SouthBayTech.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by
(qxing-at-uno.edu) from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
Thursday, July 21, 2005 at 12:25:38
------------------------------------------------------------------------
---

Email: qxing-at-uno.edu
Name: Qingfeng Xing

Organization: University of New Orleans

Education: Graduate College

Location: New Orleans LA 70148

Question: How to index high order Kikuchi lines of silicon?

Clear high order Kikuchi lines can be obtained by CBED at an orientation
far from low-index zones. I found that it's difficult to index them even
knowing the position of the direct beam on the Kickuchi map, as there
are so many lines. Is there any procedure to do that?

Thank you very much.

------------------------------------------------------------------------
---

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From: rbeavers-at-mail.smu.edu
Date: Fri, 22 Jul 2005 12:24:38 -0500
Subject: [Microscopy] Environmental SEM in North Texas

Contents Retrieved from Microscopy Listserver Archives
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Group,

Trying to locate an environmental SEM (meaning I want to look at materials that have liquid solutions as a component) in the North Texas area.

Thanks

Roy Beavers

Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



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From: jpflugheber-at-stlawu.edu
Date: Fri, 22 Jul 2005 13:08:22 -0500
Subject: [Microscopy] Re: Environmental SEM in North Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

See if these guys can help you out:
Molecular and Cellular Imaging Facility
K1 Building
University of Texas Southwestern Medical Center at Dallas
5323 Harry Hines Blvd
Dallas, TX 5390-9039



Office for Christopher Gilpin Ph.D. 214.648.2827

Lab for George Lawton
or Tom Januszewski (214) 648-7291


rbeavers-at-mail.smu.edu wrote:

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} ----------------------------------------------------------------------------
}
} Group,
}
} Trying to locate an environmental SEM (meaning I want to look at materials that have liquid solutions as a component) in the North Texas area.
}
} Thanks
}
} Roy Beavers
}
} Southern Methodist University
} Department of Geological Sciences
} P.O. Box 750395
} Dallas, TX 75275
} Voice: 214-768-2756
} Fax: 214-768-2701
} Email: rbeavers-at-smu.edu
}
}
}
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From: jmkrupp-at-cats.ucsc.edu
Date: Fri, 22 Jul 2005 13:45:46 -0500
Subject: [Microscopy] reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

Anybody remember how to reset the computer on a JEOL 1200EX? Its battery
died and it has lost its mind.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: lherault-at-bu.edu
Date: Fri, 22 Jul 2005 13:53:15 -0500
Subject: [Microscopy] reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
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I'm sure that once you replace the battery, JEOL will be able to help you
over the phone. They were always very accommodating when we had a JSM 35,
even when it was not under contract.

Ron L

-----Original Message-----
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Sent: Friday, July 22, 2005 2:50 PM
To: lherault-at-bu.edu

Hi:

Anybody remember how to reset the computer on a JEOL 1200EX? Its battery
died and it has lost its mind.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: jmkrupp-at-cats.ucsc.edu
Date: Fri, 22 Jul 2005 14:17:44 -0500
Subject: [Microscopy] oops, make that a 1200EX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

Anybody remember how to reset the computer on a JEOL 1200EX II? Its battery
died and it has lost its mind.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: Tom.Januszewski-at-UTSouthwestern.edu
Date: Fri, 22 Jul 2005 14:19:25 -0500
Subject: [Microscopy] Re: reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
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Hi Jon,
One thing that helps when we get odd characters showing up on our 1200EX
display is to hit control and A simultaneously. This puts an asterisk on
the top of page. Then simply type RESET then hit return.
Let me know if you need a hard reboot. I can give you info on that as well.
Good luck.
Tom

--
Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
Dallas, TX 75390-9039
214-648-7291
214-648-6408 (FAX)
tom.januszewski-at-UTSouthwestern.edu



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From: clei-at-uiuc.edu
Date: Fri, 22 Jul 2005 14:58:54 -0500
Subject: [Microscopy] AskAMicroscopist: How

Contents Retrieved from Microscopy Listserver Archives
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The book "Electron microdiffraction" by J.M. Zuo and J. Spence
had such program too.

Ch


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} Date: Fri, 22 Jul 2005 12:08:48 -0500
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} Subject: [Microscopy] RE: AskAMicroscopist: How to index high
order Kikuchi lines of
} To: clei-at-uiuc.edu
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From: sryazant-at-ucla.edu
Date: Fri, 22 Jul 2005 15:51:30 -0500
Subject: [Microscopy] reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
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As far as I remember, reset will erase information about pole-piece and
perhaps some other information from the computer memory, so you need to
write it down before resetting. I would try just to turn off-on computer
first (there is switch for it if you open back panel of the right console
(where computer is)). Sergey

At 12:20 PM 7/22/2005, you wrote:



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From: eoptics-at-mcmaster.ca
Date: Fri, 22 Jul 2005 17:36:12 -0500
Subject: [Microscopy] viaWWW: Making Nickel Evaporated Films

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eoptics-at-mcmaster.ca) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, July 22, 2005 at 12:43:58
---------------------------------------------------------------------------

Email: eoptics-at-mcmaster.ca
Name: Fred Pearson

Organization: McMaster University

Title-Subject: [Filtered] MListserver: Making Nickel Evaporated Films

Question: Good day

I have been trying to produce films by evaporating nickel onto a glass slide. I first wash a 1"x2" glass slide, dry it thoroughly then smear a very thin soap film across the slide and wipe it until the almost disappears. I then evaporate the nickel. I then score the slide with a sharp needle and immerse it into distilled water at about a 30 degree angle. The film floats in sections. I then scoop up the pieces with 200 um. aluminum grids.

The problem is that when it begins to dry, the film pops within each of the grid squares and disappears.

What I am I doing wrong??? Is the film not thick enough?

I have made other types of films with this exact same procedure with no problems.

thanks in advance

Fred

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From: tivol-at-caltech.edu
Date: Fri, 22 Jul 2005 19:36:16 -0500
Subject: [Microscopy] Re: viaWWW: Making Nickel Evaporated Films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jul 22, 2005, at 3:36 PM, eoptics-at-mcmaster.ca wrote:

} I have been trying to produce films by evaporating nickel onto a glass
} slide. I first wash a 1"x2" glass slide, dry it thoroughly then smear
} a very thin soap film across the slide and wipe it until the almost
} disappears. I then evaporate the nickel. I then score the slide with a
} sharp needle and immerse it into distilled water at about a 30 degree
} angle. The film floats in sections. I then scoop up the pieces with
} 200 um. aluminum grids.
}
} The problem is that when it begins to dry, the film pops within each
} of the grid squares and disappears.
}
} What I am I doing wrong??? Is the film not thick enough?
}
} I have made other types of films with this exact same procedure with
} no problems.
}
Dear Fred,
If the film breaks apart as it is floated off--assuming that is what
you mean by "in sections"--then it may not be structurally strong
enough. You don't say how thick the film is, so I don't have a feeling
whether making it thicker will solve the problem. One thing you could
try is to take the wet grid and put it onto a drop of ethanol, which
will lower the surface tension, and that might reduce the forces that
pop the film. It may be that the evaporated nickel is too crystalline
with relatively weak cohesion between crystals, making it weaker than
might be expected. A possible alternative is to put a formvar coat
onto the grids, evaporate the Ni onto the formvar, then dissolve the
formvar away with CHCl3. If you try this, be careful not to let pools
of CHCl3 touch the grids, but put them on a piece of filter paper
uphill from the CHCl3 and let only the liquid drawn up to the grids
through the paper touch the formvar--the same technique for dissolving
formvar from under a layer of C. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: cgarber-at-2spi.com
Date: Sat, 23 Jul 2005 13:36:46 -0500
Subject: [Microscopy] Making Ni films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fred Pearson wrote:
====================================================
I have been trying to produce films by evaporating nickel onto a glass
slide. I first wash a 1"x2" glass slide, dry it thoroughly then smear a very
thin soap film across the slide and wipe it until the almost disappears. I
then evaporate the nickel. I then score the slide with a sharp needle and
immerse it into distilled water at about a 30 degree angle. The film floats
in sections. I then scoop up the pieces with 200 um. aluminum grids.

The problem is that when it begins to dry, the film pops within each of the
grid squares and disappears.

What I am I doing wrong??? Is the film not thick enough?

I have made other types of films with this exact same procedure with no
problems.
===================================================
You did not mention how you were applying the Ni films, if you are trying to
do this by sputtering, you would need a truly clean and UHV environment or
else, so I have been led to believe, you will end up with a significant
amount of oxide.

My biggest concern would be with the smearing of the "very thin soap film
across the slide". What looks "disappeared" to the eye could be big globs
so far as TEM observation dimensions are concerned (if transferred to the
film) and that could lead to film instability in the electron beam.

An alternative approach might be to evaporate Victawet® (if you are not
familiar with this interesting surfactant, see URL
http://www.2spi.com/catalog/spec_prep/evapor_3c.shtml

By the vacuum evaporation of Victawet, you can apply a more thinner and more
uniform layer than you could ever get by "wiping" and experience has been
that whatever Victawet that might be left (it will dissolve in water) won't
be sensitive in the presence of the electron beam.

We have ourselves not tried Victawet with Ni but have used it quite
successfully with other coating materials and it really does work.

Disclaimer: SPI Supplies is a supplier of Victawet for electron microscopy
so we would have a vested interest in having more people using Victawet.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================








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From: richard-at-torland.demon.co.uk
Date: Sun, 24 Jul 2005 06:07:04 -0500
Subject: [Microscopy] Re: reset JEOL 1200EX?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

it is quite easy to perform a full reset on a 1200 II. You will however
lose a lot of information, for example type of vacuum system, type of
polepiece and other serious data that the microscope needs to operate
correctly, I would suggest you contact your local Jeol office who can give
instructions over the phone,

Richard Hey

Principal Technical Support Engineer
Jeol (UK) Ltd.



The views expressed herein do not reflect the views of Jeol
























At 15:56 22/07/2005 -0500, you wrote:



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From: philf-at-newton.umsl.edu
Date: Sun, 24 Jul 2005 10:31:14 -0500
Subject: [Microscopy] viaWWW: Nano-cluster web-lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (philf-at-newton.umsl.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Saturday, July 23, 2005 at 14:43:34
---------------------------------------------------------------------------

Email: philf-at-newton.umsl.edu
Name: Phil Fraundorf

Organization: UM-StL Physics and Astronomy/CME

Title-Subject: [Filtered] MListserver: Nano-cluster web-lab

Question: Hi,

We're developing a webpage* for simulation and
analysis of imaging, diffraction, and spectroscopic
data inspired by the fact that for small numbers of
atoms these effects are easier to model. One can
already use it to index cross-fringe lattice
images and single-crystal diffraction patterns
against some candidate structures, and to generate
projected-potential and truncated Debye-sum
diffraction images down any orientation. The latter
allow one to quantify finite-crystal effects,
e.g. of reduced specimen thickness in directions
perpendicular to a g-vector. This gives rise to
high-frequency "fore-shortening tails" in powder
patterns, even as reduced-thickness parallel to
the same g-vector broadens diffraction spots
symmetrically. These effects are quite important
in the study, for example, of data from graphene
nano-structures**.

As far as education is concerned, this virtual
goniometer will offer your students a chance to
take and ponder data from unknown nanostructures,
without the cost of scope time. Moreover, for
those into diffraction, specimen-tilting while
viewing from the side also nicely illustrates a
flat Ewald-sphere in action.

Suggestions and corrections (we're just getting
started) to philf-at-newton.umsl.edu are invited.
Except for this announcement, the system is too
much in its infancy to warrant much bandwidth
on the listserver. However, you might want to
bookmark it, as we hope to develop it into a
reliable resource for research and teaching in days
ahead.

Thanx! /phil

* http://www.umsl.edu/~fraundor/nanowrld/newlive/crystal3.html
** cf. ApJLett 578 (2002) L153 & Warren (1941) PhysRev v59n9p693.

Phil Fraundorf
UM-StL Physics & Astronomy/CME


---------------------------------------------------------------------------

==============================Original Headers==============================
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From: beth-at-plantbio.uga.edu
Date: Mon, 25 Jul 2005 10:29:34 -0500
Subject: [Microscopy] "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ok, so this is not serious journalism but it was in the popular press -
did anyone else read Parade columnist Marilyn vos Savant's article last
Sunday "Are men smarter than women?"? The article is mainly about women
in science or the lack thereof. About half way through the article she
decides to bash microscopists:

"And note that dictators - who aren't any stronger than other men - are
never women. Maybe females just don't have whatever it takes to
bulldoze their way to this dubious sort of "success". No one thinks the
paucity of women in the field of ruthless domination is because they
aren't smart enough! So why should anyone be shocked to find that most
bright people - including women - would flee from the sight of a
microscope?!"

Marilyn is in the Guinness Book of Records Hall of Fame for having the
highest IQ ever recorded. If you feel so inclined to enlighten Marilyn
about microscopy (or bad analogies) you can email her at
{marilyn-at-parade.com} .

see you "not so bright people" in Honolulu,
Beth

PS - she is intelligent enough to say that neither gender is smarter
than the other.

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



==============================Original Headers==============================
12, 18 -- From beth-at-plantbio.uga.edu Mon Jul 25 10:29:34 2005
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From: hoffpajo-at-yahoo.com
Date: Mon, 25 Jul 2005 10:37:54 -0500
Subject: [Microscopy] Re: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

well this is a good 2 minutes of my life i will never
get back, trume there haven't benn any women
dictators, they just attach themselves to ditators.

--- beth-at-plantbio.uga.edu wrote:

}
}
}
}
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} Microscopy Society of America
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}
} Ok, so this is not serious journalism but it was in
} the popular press -
} did anyone else read Parade columnist Marilyn vos
} Savant's article last
} Sunday "Are men smarter than women?"? The article is
} mainly about women
} in science or the lack thereof. About half way
} through the article she
} decides to bash microscopists:
}
} "And note that dictators - who aren't any stronger
} than other men - are
} never women. Maybe females just don't have whatever
} it takes to
} bulldoze their way to this dubious sort of
} "success". No one thinks the
} paucity of women in the field of ruthless domination
} is because they
} aren't smart enough! So why should anyone be shocked
} to find that most
} bright people - including women - would flee from
} the sight of a
} microscope?!"
}
} Marilyn is in the Guinness Book of Records Hall of
} Fame for having the
} highest IQ ever recorded. If you feel so inclined to
} enlighten Marilyn
} about microscopy (or bad analogies) you can email
} her at
} {marilyn-at-parade.com} .
}
} see you "not so bright people" in Honolulu,
} Beth
}
} PS - she is intelligent enough to say that neither
} gender is smarter
} than the other.
}
}
**********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
} http://www.plantbio.uga.edu/emlab
}
} "Between the two evils,
} I always pick the one I never tried before". Mae
} West (1893-1980)
}
*******************************************************************
}
} "And it's only the giving that makes you what you
} are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
}
************************************************************************
}
} ***
}
}
}
} ==============================Original
} Headers==============================
} 12, 18 -- From beth-at-plantbio.uga.edu Mon Jul 25
} 10:29:34 2005
} 12, 18 -- Received: from dogwood.plantbio.uga.edu
} (dogwood.plantbio.uga.edu [128.192.26.2])
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} with ESMTP id j6PFTXIa021865
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} Jul 2005 10:29:33 -0500
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} DES-CBC3-SHA (168 bits))
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} charset=US-ASCII; format=flowed
} 12, 18 -- Subject: "most bright people"
} 12, 18 -- From: Beth Richardson
} {beth-at-plantbio.uga.edu}
} 12, 18 -- To: microscopy {microscopy-at-microscopy.com}
} 12, 18 -- Content-Transfer-Encoding: 7bit
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}
{E4EE6FFB-FD20-11D9-8C24-000393137C00-at-plantbio.uga.edu}
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} ==============================End of -
} Headers==============================
}


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From: hinmeigeng-at-hotmail.com
Date: Mon, 25 Jul 2005 15:43:59 -0500
Subject: [Microscopy] ESEM of soil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Any advice on the following?

======
A student working on a Geoarchaeology masters dissertation writes:

I would like to use the environmental SEM soon after
the centre re-opens on the 8th August. I have potentially up to 30 soil and
sediment samples that I am hoping consist of loess to some degree or other.
My plan is to examine the quartz grains in the samples for evidence of
frost-shattering patterns. I've never used this kind of equipment before and
I would very much appreciate any advice you may have regarding preparing
these samples for viewing. Sample size, condition, mounting etc.

======
Thanks for your time,

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



==============================Original Headers==============================
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From: lcgould-at-med.cornell.edu
Date: Mon, 25 Jul 2005 15:52:01 -0500
Subject: [Microscopy] gummy blocks-summary of responses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi and thanks to all of you who responded to my question about gummy
blocks. Here is a summary of the suggestions you sent:

-extra changes of absolute ethanol
-lower temp, longer cure
-more gradations of ethanol-epon mix leading up to pure resin, with
agitation at all steps
-use fresh accelerator ( {6 months old) and switch to BDMA instead of DMP-30
-use HPMA (Hydroypropyl Methacrylate) in place of PO as a final
dehydrating agent, and then stepwise into Epon (it won't eat the
plastic dishes)
-use polypropylene culture dishes
-remove the cover glass and embed in Chang mold

The last 2 suggestions won't work for me. The dishes come in just 1
"flavor" and if I could remove the cover glass without destroying it,
I would have. These are attached with Sylgard (I think), and its a
tight bond.

Thanks again,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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From: john.mardinly-at-intel.com
Date: Mon, 25 Jul 2005 17:27:55 -0500
Subject: [Microscopy] "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Isn't this the same Marilyn vos Savant who wrote in her column that a
bullet fired from a high powered gun into the air was harmless when it
came back down? I just want to know who did the IQ testing.

John Mardinly
Intel

The opinion of this author is not necessarily the opinion of Intel corp.

-----Original Message-----
X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu]
Sent: Monday, July 25, 2005 8:30 AM
To: Mardinly, John

Ok, so this is not serious journalism but it was in the popular press -

did anyone else read Parade columnist Marilyn vos Savant's article last

Sunday "Are men smarter than women?"? The article is mainly about women

in science or the lack thereof. About half way through the article she
decides to bash microscopists:

"And note that dictators - who aren't any stronger than other men - are

never women. Maybe females just don't have whatever it takes to
bulldoze their way to this dubious sort of "success". No one thinks the

paucity of women in the field of ruthless domination is because they
aren't smart enough! So why should anyone be shocked to find that most
bright people - including women - would flee from the sight of a
microscope?!"

Marilyn is in the Guinness Book of Records Hall of Fame for having the
highest IQ ever recorded. If you feel so inclined to enlighten Marilyn
about microscopy (or bad analogies) you can email her at
{marilyn-at-parade.com} .

see you "not so bright people" in Honolulu,
Beth

PS - she is intelligent enough to say that neither gender is smarter
than the other.

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************

***



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From: ard-at-ansto.gov.au
Date: Mon, 25 Jul 2005 18:37:26 -0500
Subject: [Microscopy] RE: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well who knows. Perhaps Marilyn has a point about the lack of female
dictators through history. But that's not to say that women haven't
caused just as much damage. Take Helen of Troy for example. (Joke)

Since when was there ever a positive correlation between so-called
intelligence and the abuse of opportunistic bigotry? Marilyn's
mission is to help her employer's media company make money. Thus for
the good of all this sort of crap journalism should just be ignored.

Anyway, aren't IQ tests supposed to be culture and gender biased in
favor of white western males? Has anyone seen a photo of "Marilyn"?


}
} "And note that dictators - who aren't any stronger than other men - are
}
} never women. Maybe females just don't have whatever it takes to
} bulldoze their way to this dubious sort of "success". No one thinks the
}
} paucity of women in the field of ruthless domination is because they
} aren't smart enough! So why should anyone be shocked to find that most
} bright people - including women - would flee from the sight of a
} microscope?!"
}
} Marilyn is in the Guinness Book of Records Hall of Fame for having the
} highest IQ ever recorded. If you feel so inclined to enlighten Marilyn
} about microscopy (or bad analogies) you can email her at
} {marilyn-at-parade.com} .


==============================Original Headers==============================
7, 26 -- From ard-at-ansto.gov.au Mon Jul 25 18:37:23 2005
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From: hoffpajo-at-yahoo.com
Date: Mon, 25 Jul 2005 19:27:32 -0500
Subject: [Microscopy] Re: RE: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

women throughout history have latched themselves on to
men od power, must i mention eva braum.
i am not certain as to why beth would even bring this
up, as far as i can see Marilyn's form of journalism
is self serving crap. i am not even convinced the
letters wrtten to her are real.
so i ask beth, what was your motivation?

--- ard-at-ansto.gov.au wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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} http://www.microscopy.com/MicroscopyListserver
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}
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}
}
} Well who knows. Perhaps Marilyn has a point about
} the lack of female
} dictators through history. But that's not to say
} that women haven't
} caused just as much damage. Take Helen of Troy for
} example. (Joke)
}
} Since when was there ever a positive correlation
} between so-called
} intelligence and the abuse of opportunistic bigotry?
} Marilyn's
} mission is to help her employer's media company make
} money. Thus for
} the good of all this sort of crap journalism should
} just be ignored.
}
} Anyway, aren't IQ tests supposed to be culture and
} gender biased in
} favor of white western males? Has anyone seen a
} photo of "Marilyn"?
}
}
} }
} } "And note that dictators - who aren't any stronger
} than other men - are
} }
} } never women. Maybe females just don't have whatever
} it takes to
} } bulldoze their way to this dubious sort of
} "success". No one thinks the
} }
} } paucity of women in the field of ruthless
} domination is because they
} } aren't smart enough! So why should anyone be
} shocked to find that most
} } bright people - including women - would flee from
} the sight of a
} } microscope?!"
} }
} } Marilyn is in the Guinness Book of Records Hall of
} Fame for having the
} } highest IQ ever recorded. If you feel so inclined
} to enlighten Marilyn
} } about microscopy (or bad analogies) you can email
} her at
} } {marilyn-at-parade.com} .
}
}
} ==============================Original
} Headers==============================
} 7, 26 -- From ard-at-ansto.gov.au Mon Jul 25 18:37:23
} 2005
} 7, 26 -- Received: from tachyon.gw.ansto.gov.au
} (tachyon.gw.ansto.gov.au [137.157.8.253])
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} ESMTP id j6PNbMwO001736
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} Jul 2005 18:37:23 -0500
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} (8.11.7p1+Sun/8.11.7) id j6PNbLn10794
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} Jul 2005 09:37:21 +1000 (EST)
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} 7, 26 -- Date: Tue, 26 Jul 2005 09:37:17 +1000
} 7, 26 -- To: microscopy-at-microscopy.com
} 7, 26 -- From: Arthur Day {ard-at-ansto.gov.au}
} 7, 26 -- Subject: [Microscopy] RE: "most bright
} people"
} 7, 26 -- Content-Type: text/plain;
} charset="us-ascii" ; format="flowed"
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} ==============================End of -
} Headers==============================
}


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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Mon Jul 25 19:27:32 2005
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5, 19 -- Subject: Re: [Microscopy] RE: "most bright people"
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From: zaluzec-at-microscopy.com
Date: Mon, 25 Jul 2005 19:37:05 -0500
Subject: [Microscopy] Administrivia: most bright people

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

This thread is starting to seriously drift off microscopy related themes.
Let's let this one die, before it get too tangential.

Nestor
Your Friendly Neighborhood SysOp

==============================Original Headers==============================
3, 13 -- From zaluzec-at-microscopy.com Mon Jul 25 19:37:05 2005
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3, 13 -- Date: Mon, 25 Jul 2005 19:37:03 -0500
3, 13 -- To: microscopy-at-microscopy.com
3, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
3, 13 -- Subject: Administrivia: most bright people
3, 13 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: avklaus-at-amnh.org
Date: Mon, 25 Jul 2005 19:39:23 -0500
Subject: [Microscopy] RE: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My guess is that Beth thought this was pretty funny and wanted to share it
with her friends and colleagues who she is really looking forward to
seeing next week. If that's the case, I must agree.

Just a guess. I'm looking forward to meeting you Beth!

All best,

Angela
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} women throughout history have latched themselves on to
} men od power, must i mention eva braum.
} i am not certain as to why beth would even bring this
} up, as far as i can see Marilyn's form of journalism
} is self serving crap. i am not even convinced the
} letters wrtten to her are real.
} so i ask beth, what was your motivation?
}
} --- ard-at-ansto.gov.au wrote:
}
} }
} }
} }
} }
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} }
} } Well who knows. Perhaps Marilyn has a point about
} } the lack of female
} } dictators through history. But that's not to say
} } that women haven't
} } caused just as much damage. Take Helen of Troy for
} } example. (Joke)
} }
} } Since when was there ever a positive correlation
} } between so-called
} } intelligence and the abuse of opportunistic bigotry?
} } Marilyn's
} } mission is to help her employer's media company make
} } money. Thus for
} } the good of all this sort of crap journalism should
} } just be ignored.
} }
} } Anyway, aren't IQ tests supposed to be culture and
} } gender biased in
} } favor of white western males? Has anyone seen a
} } photo of "Marilyn"?
} }
} }
} } }
} } } "And note that dictators - who aren't any stronger
} } than other men - are
} } }
} } } never women. Maybe females just don't have whatever
} } it takes to
} } } bulldoze their way to this dubious sort of
} } "success". No one thinks the
} } }
} } } paucity of women in the field of ruthless
} } domination is because they
} } } aren't smart enough! So why should anyone be
} } shocked to find that most
} } } bright people - including women - would flee from
} } the sight of a
} } } microscope?!"
} } }
} } } Marilyn is in the Guinness Book of Records Hall of
} } Fame for having the
} } } highest IQ ever recorded. If you feel so inclined
} } to enlighten Marilyn
} } } about microscopy (or bad analogies) you can email
} } her at
} } } {marilyn-at-parade.com} .
} }
} }
} } ==============================Original
} } Headers==============================
} } 7, 26 -- From ard-at-ansto.gov.au Mon Jul 25 18:37:23
} } 2005
} } 7, 26 -- Received: from tachyon.gw.ansto.gov.au
} } (tachyon.gw.ansto.gov.au [137.157.8.253])
} } 7, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with
} } ESMTP id j6PNbMwO001736
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} } Jul 2005 18:37:23 -0500
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} } (8.11.7p1+Sun/8.11.7) id j6PNbLn10794
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} } Jul 2005 09:37:21 +1000 (EST)
} } 7, 26 -- Received: from
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} } 7, 26 -- Date: Tue, 26 Jul 2005 09:37:17 +1000
} } 7, 26 -- To: microscopy-at-microscopy.com
} } 7, 26 -- From: Arthur Day {ard-at-ansto.gov.au}
} } 7, 26 -- Subject: [Microscopy] RE: "most bright
} } people"
} } 7, 26 -- Content-Type: text/plain;
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--
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-796-5977
Fax: 212-496-3480


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From: gary-at-gaugler.com
Date: Mon, 25 Jul 2005 19:56:45 -0500
Subject: [Microscopy] Re: "most bright people"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ahhh... well, then I suppose that we won't see her
at M&M 2005. That is too bad. It would be great
to get her enlightened input on EBSD, CL, EDS,
TEM, STEM, DIC, PH, BF, DF, and other EM/LM issues/topics.

I don't see a strong (or any) correlation between IQ
and microscopy. If one is in microscopy, I assume
that this is because they love it. Passion is not IQ.
Focus is not IQ. Some are for a job. But then too,
they do it. And if poorly done, they are done.

OK. So I'm passionate about EM. Get Marilyn here at
M&M 2005 and let's talk about this topic. Otherwise,
relegate her to history. There are way too many other
important topics to worry about then her quantitative IQ.

Regardless of my personal IQ (Intelligence Quotient), my
Interest Quotient (IQ2) for EM is very high. So I would
go face-to-face with her on EM. That would be fun.

gary g.


At 08:34 AM 7/25/2005, you wrote:



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From: liz.girvan-at-stonebow.otago.ac.nz
Date: Mon, 25 Jul 2005 23:41:19 -0500
Subject: [Microscopy] Sputter Coater help for Dunedin!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steve
Hope all is well over there. Great excitement here in SEM land - we
have just got our new Emitech K575X Sputter Coater set up. Allan
suggested I email and see if you had any brilliant idea as to what
kind of test specimen I could use to try it out.
You may also have some advice about how to use properly?
Am still enjoying the Protrain course - getting there slowly!
Thanks for your help
Kind regards
Liz
PS Allan said you're coming down here next July - looking forward to it!
--

------------------------------------------------------------------------------------------------------------------------
Liz Girvan
Electron Microscopist

Otago Centre for Electron Microscopy
C/- Department of Anatomy and Structural Biology
Otago School of Medical Sciences
PO Box 913
Dunedin
New Zealand
Phone (03) 479 7386

http://ocem.otago.ac.nz/

**Ride the Lightning**

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From: innap-at-savion.huji.ac.il
Date: Tue, 26 Jul 2005 01:34:58 -0500
Subject: [Microscopy] ESEM of soil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Robert,
For the beginning you can put a piece of your sample on the standard SEM
metal stub (aroung 12 mm diameter) coated with carbon tape (conductive
carbon glue - also standard for SEM) and use Low Vacuum mode for
studying the morphology of your samples/sediments. When looking at the
micro structural features of the samples and seeing something like
grains, which you can attribute to quartz or can not, you start EDS
acquisitions for measuring their composition. And so on and so on.
First, you will find proper LV conditions for observation (HT, pressure
and spot size) with Gaseous SE detector. Then, in order to separate
heavy and light elements containing features you can use BSE detector.
And then you can measure the composition with EDS. If you want to go up
to the quantification, you have to acquire spectra from the same region
at two different pressure values, because only in this way you will be
able extract a right chemical information.
Good Luck,
Best,
Inna

Dr. Inna Popov
Head of The Unit for Nanoscopic Characterization
The Hebrew University of Jerusalem
E.Safra Campus Givat Ram
Jerusalem 91904
ISRAEL
www.nanoscience.huji.ac.il/unit
Tel: +972 2 6584808
Fax: +972 2 6584809
email: innap-at-savion.huji.ac.il

-----Original Message-----
X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com]
Sent: Monday, July 25, 2005 11:47 PM
To: Inna Popov

Dear Listers,

Any advice on the following?

======
A student working on a Geoarchaeology masters dissertation writes:

I would like to use the environmental SEM soon after
the centre re-opens on the 8th August. I have potentially up to 30 soil
and
sediment samples that I am hoping consist of loess to some degree or
other.
My plan is to examine the quartz grains in the samples for evidence of
frost-shattering patterns. I've never used this kind of equipment before
and
I would very much appreciate any advice you may have regarding preparing
these samples for viewing. Sample size, condition, mounting etc.

======
Thanks for your time,

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



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From: mccaulak-at-wfu.edu
Date: Tue, 26 Jul 2005 08:50:03 -0500
Subject: [Microscopy] viaWWW: user charges for SEM and related equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Liz

How about taking a ~0.25 latex and diluting it until you have an even
monolayer (~30:1 distilled water). Then try coating you should NOT be able
to visualise ANY structure!

You need to remove the chromium oxide prior to applying a coat. Shutter the
target and sputter until the plasma reaches a very nice light blue colour
(once seen never forgotten) then remove the shutter and coat for just a few
seconds.

Good luck as my information is how we ran the prototype so there may be a
better route today?

Regards

Steve

PS see you in July it seems - great

----- Original Message -----
X-from: {liz.girvan-at-stonebow.otago.ac.nz}
To: {protrain-at-emcourses.com}
Sent: Tuesday, July 26, 2005 5:42 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005 at 12:09:42
---------------------------------------------------------------------------

Email: mccaulak-at-wfu.edu
Name: Anita McCauley

Organization: Wake Forest University

Title-Subject: [Filtered] MListserver: user charges for SEM and related equipment

Question: I run a small microscopy facility in which equipment is normally made available to users free of charge. We are currently developing a relationship with an outside user and would like to generate a usage fee list so that we can recover costs for equipment wear and consumables.

Rather than pulling numbers out of thin air, I would like to know what others charge for the use of an SEM, a critical point dryer, and a sputter coater. Tech time does not need to be included. Hopefully with this information, I will be able to put together a reasonable usage fee list.

Thanks.

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: mccaulak-at-wfu.edu
Date: Tue, 26 Jul 2005 08:50:22 -0500
Subject: [Microscopy] viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005 at 12:19:54
---------------------------------------------------------------------------

Email: mccaulak-at-wfu.edu
Name: Anita McCauley

Organization: Wake Forest University

Title-Subject: [Filtered] MListserver: video camera for stereomicroscope

Question: I am looking to add a video camera to an existing Olympus SZX12 stereomicroscope. I would like to consider cameras ranging from consumer-grade cameras bought at big-box electronic stores to those made for scientific applications. Currently, I am having trouble finding much information regarding scientific-grade video cameras.

I would appreciate hearing from folks that have been using either type of camera on a stereomicroscope, the pros and cons to their set-ups, and whether they are happy with their configuration.

Thanks for the help.

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: Sven.Terclavers-at-med.kuleuven.be
Date: Tue, 26 Jul 2005 09:14:08 -0500
Subject: [Microscopy] Re: viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Anita,

I've been using two different, commercially available Sony digital
videocameras with success on both Zeiss and Leica stereomicroscopes.
The only disadvantage I experienced is that the cameras have
difficulties when filming samples which have both dark and very light
spots. However, with a little rearrangment of the light setup, things
could be quite solved! It of course also depends on the purpose:
analysis or imaging. But for imagingm the quality is more than enough
to be accepted by top-rated journals!
Best,

Sven Terclavers



Quoting mccaulak-at-wfu.edu:

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}
} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: video camera for
} stereomicroscope
}
} Question: I am looking to add a video camera to an existing Olympus
} SZX12 stereomicroscope. I would like to consider cameras ranging
} from consumer-grade cameras bought at big-box electronic stores to
} those made for scientific applications. Currently, I am having
} trouble finding much information regarding scientific-grade video
} cameras.
}
} I would appreciate hearing from folks that have been using either
} type of camera on a stereomicroscope, the pros and cons to their
} set-ups, and whether they are happy with their configuration.
}
} Thanks for the help.
}
}
----------------------------------------------------------------------
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}
} ==============================Original
} Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:22 2005
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} 8, 12 -- Subject: viaWWW: video camera for stereomicroscope
} 8, 12 -- Content-Type: text/plain; charset="us-ascii"
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}
}




==============================Original Headers==============================
10, 29 -- From Sven.Terclavers-at-med.kuleuven.be Tue Jul 26 09:14:07 2005
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10, 29 -- Date: Tue, 26 Jul 2005 16:13:51 +0200
10, 29 -- From: Sven Terclavers {Sven.Terclavers-at-med.kuleuven.be}
10, 29 -- To: microscopy-at-microscopy.com
10, 29 -- Subject: Re: [Microscopy] viaWWW: video camera for stereomicroscope
10, 29 -- References: {200507261353.j6QDrUIE020974-at-ns.microscopy.com}
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From: skod-at-ises-llc.com
Date: Tue, 26 Jul 2005 10:24:01 -0500
Subject: [Microscopy] Would like to exchange email with experienced Leitz AF users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been in the (possibly foolish) process of restoring a 1991-era
Leitz AMC AF microscope with the intention of building up an automated
image capture/tiling system. Things have been going moderately well, and
most of the system is finally ready to go. However, I've encountered a
curious problem: the microscope controller is not particularly
configurable, and will not release the autofocus unit for normal function
until *after* it has gone completely through its stage homing procedure.
And, since I have changed over to a stepper stage and a more modern
PC-based controller, the old servo-driven stage is no longer even present
in the system.

I have obtained all the documentation that is available from Leitz, and
have been through it with a fine-toothed comb: no mention is ever made of
using only the autofocus as a standalone unit. If anyone has any
experience with hacking on the various mid-90s Leitz AF hardware in any of
its many manifestations (the AMC AF, CDV, LIS, MPV-SP), I'd very much like
to spend a few minutes picking your brain via email or telephone.

My plan B is to simply scrap the AF unit altogether and go with the
digital video based solution offered by the new controller. However, if I
can save myself a few hundred dollars (and justify having purchased the
unit to start with!), I'd like to press the original AF hardware into use
if possible. Many thanks in advance for anyone willing to spend a few
minutes on this topic: please contact me offlist at skod-at-ises-llc.com.

Scott Griffith
ISES-LLC
9745 Steeplechase Drive
Franktown, CO 80116
303-660-2541 voice
303-660-2542 fax
skod-at-ises-llc.com


==============================Original Headers==============================
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5, 17 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005 09:06:41 -0600 (MDT)
5, 17 -- To: microscopy-at-microscopy.com
5, 17 -- Subject: Would like to exchange email with experienced Leitz AF users
5, 17 -- Date: Tue, 26 Jul 2005 09:23:54 -0600
5, 17 -- From: "Scott Griffith" {skod-at-ises-llc.com}
5, 17 -- Organization: ISES-LLC
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From: amenex-at-amenex.com
Date: Tue, 26 Jul 2005 10:38:02 -0500
Subject: [Microscopy] Re: user charges for SEM and related equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Ms. McCauley:

You asked:
} Question: ... snipppage ... We are currently developing a
} relationship with an outside user and would like to generate
} a usage fee list so that we can recover costs for equipment
} wear and consumables.
}
} Rather than pulling numbers out of thin air, I would like to
} know what others charge for the use of an SEM, a critical point
} dryer, and a sputter coater. Tech time does not need to be
} included. Hopefully with this information, I will be able to
} put together a reasonable usage fee list.

Salaries and overhead ? If you don't recover _all_ costs, then
your charges will be below actual cost to your funding agencies
and therefore unfair to private microscopy labs that have to
make a profit in order to survive in the marketplace.

You need to find out your true costs. Asking others to tell you
the going rates amounts to a combination in restraint of trade
between you and whoever responds or fails to object to this unfair
trade practice. There are still antitrust laws in effect, I think.

Best regards,
George Langford, Sc.D.
Principal Consultant
Amenex Associates, Inc.
http://www.amenex.com/
amenex-at-amenex.com



==============================Original Headers==============================
7, 24 -- From amenex-at-amenex.com Tue Jul 26 10:38:01 2005
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7, 24 -- To: "Anita McCauley" {mccaulak-at-wfu.edu}
7, 24 -- CC: microscopy-at-microscopy.com, amenex-at-amenex.com
7, 24 -- Subject: Re: user charges for SEM and related equipment
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From: mccaulak-at-wfu.edu
Date: Tue, 26 Jul 2005 10:56:57 -0500
Subject: [Microscopy] Re: viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for the feedback...I have been looking at some Sony MiniDVs and so
its good to hear that folks have been able to use them successfully.

Anita K. McCauley, Ph.D.
Director of Microscopy/Adj. Asst. Prof.
PO Box 7325
Biology Department
Wake Forest University
Winston-Salem, NC 27109


-----Original Message-----
X-from: Sven.Terclavers-at-med.kuleuven.be
[mailto:Sven.Terclavers-at-med.kuleuven.be]
Sent: Tuesday, July 26, 2005 10:17 AM
To: mccaulak-at-wfu.edu

Dear Anita,

I've been using two different, commercially available Sony digital
videocameras with success on both Zeiss and Leica stereomicroscopes.
The only disadvantage I experienced is that the cameras have
difficulties when filming samples which have both dark and very light
spots. However, with a little rearrangment of the light setup, things
could be quite solved! It of course also depends on the purpose:
analysis or imaging. But for imagingm the quality is more than enough
to be accepted by top-rated journals!
Best,

Sven Terclavers



Quoting mccaulak-at-wfu.edu:

}
}
}
}
----------------------------------------------------------------------
------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (mccaulak-at-wfu.edu) from
} http://www.microscopy.com/MLFormMail.html on Monday, July 25, 2005
at
} 12:19:54
}
----------------------------------------------------------------------
-----
}
} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: video camera for
} stereomicroscope
}
} Question: I am looking to add a video camera to an existing Olympus
} SZX12 stereomicroscope. I would like to consider cameras ranging
} from consumer-grade cameras bought at big-box electronic stores to
} those made for scientific applications. Currently, I am having
} trouble finding much information regarding scientific-grade video
} cameras.
}
} I would appreciate hearing from folks that have been using either
} type of camera on a stereomicroscope, the pros and cons to their
} set-ups, and whether they are happy with their configuration.
}
} Thanks for the help.
}
}
----------------------------------------------------------------------
-----
}
} ==============================Original
} Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:22 2005
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} 8, 12 -- From: mccaulak-at-wfu.edu (by way of MicroscopyListserver)
} 8, 12 -- Subject: viaWWW: video camera for stereomicroscope
} 8, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of -
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}
}




==============================Original Headers==============================
10, 29 -- From Sven.Terclavers-at-med.kuleuven.be Tue Jul 26 09:14:07 2005
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-0500
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10, 29 -- Date: Tue, 26 Jul 2005 16:13:51 +0200
10, 29 -- From: Sven Terclavers {Sven.Terclavers-at-med.kuleuven.be}
10, 29 -- To: microscopy-at-microscopy.com
10, 29 -- Subject: Re: [Microscopy] viaWWW: video camera for
stereomicroscope
10, 29 -- References: {200507261353.j6QDrUIE020974-at-ns.microscopy.com}
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==============================Original Headers==============================
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19, 21 -- To: {microscopy-at-microscopy.com}
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From: GBurgess-at-exchange.hsc.mb.ca
Date: Tue, 26 Jul 2005 11:01:10 -0500
Subject: [Microscopy] saturated NaOH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm trying to express in grams the amount of NaOH that I'd need for 100 ml
of saturated NaOH in ethanol.

Does anyone know this off the top of their head without me having to find a
reference or figure it out with the real pellets.

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

==============================Original Headers==============================
4, 19 -- From GBurgess-at-exchange.hsc.mb.ca Tue Jul 26 11:01:10 2005
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4, 19 -- Date: Tue, 26 Jul 2005 10:59:25 -0500
4, 19 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
4, 19 -- Subject: saturated NaOH
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From: dsherman-at-purdue.edu
Date: Tue, 26 Jul 2005 11:07:59 -0500
Subject: [Microscopy] Re: viaWWW: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anita,

You do have to be careful about this topic on the list. There is a concern
(rightly so) from for-profit companies that Universities will deprive them
of business by undercutting fees. The question you ask is fairly complex.
You do need to look at all costs associated with your facility (service
contracts, consumables, telephone, etc) and come up with a rate structure
that would cover these costs if you needed to charge all current users.

Then look at your internal users as subsidized. To the base costs, add the
percentage used by your university for indirect costs. This would be the
same amount charged to grants to cover building costs, lights, heating, etc.
This can be as high as 50% in many institutions.

If you are doing the work than figure your salary, fringe benefits, etc. to
get an hourly rate that compensates totally for your time.
Tack on a few extra dollars when figuring consumable costs as prices go up
regularly and you need to recoup these costs without having to change your
whole rate structure.

For example: Assume an SEM with $15,000 service contract and consumables
(filaments, nitrogen, etc) of $2000/yr. Figure 1000 hours of billable use
time results in an hourly charge of $17/hr. Add time you spend maintaining
the instrument (aligning, changing filaments, other non-billable time adding
up to 1000hrs/yr) and this figure can easily double. Add the 50% overhead
and you arrive at $51/hr.

This amount is very low compared to what for-profit labs must charge as they
have to use capital to purchase the instrument in the first place and then
depreciate it so they will have funds to replace when necessary. They also
need to pay all costs associated with the physical structures, advertising,
etc. This is why they are concerned about Universities undercutting their
necessary charges. If you have no private labs near you than you can
probably get away with the lower costs. Industry would be delighted with
this amount/hr as their costs if they owned the machine would be much
greater due to the depreciation, etc.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



On 7/26/05 8:52 AM, "mccaulak-at-wfu.edu" {mccaulak-at-wfu.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted
} by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on
} Monday, July 25, 2005 at 12:09:42
} ---------------------------------------------------------------------------
}
} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: user charges for SEM and related
} equipment
}
} Question: I run a small microscopy facility in which equipment is normally
} made available to users free of charge. We are currently developing a
} relationship with an outside user and would like to generate a usage fee list
} so that we can recover costs for equipment wear and consumables.
}
} Rather than pulling numbers out of thin air, I would like to know what others
} charge for the use of an SEM, a critical point dryer, and a sputter coater.
} Tech time does not need to be included. Hopefully with this information, I
} will be able to put together a reasonable usage fee list.
}
} Thanks.
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Tue Jul 26 08:50:01 2005
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From: hoffpajo-at-yahoo.com
Date: Tue, 26 Jul 2005 11:12:34 -0500
Subject: [Microscopy] Re: saturated NaOH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

as i recall 52 gms of NaOH in 100ml DH20 will yield a
saturated soln
john

--- GBurgess-at-exchange.hsc.mb.ca wrote:

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} I'm trying to express in grams the amount of NaOH
} that I'd need for 100 ml
} of saturated NaOH in ethanol.
}
} Does anyone know this off the top of their head
} without me having to find a
} reference or figure it out with the real pellets.
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From: bfoster-at-mme1.com
Date: Tue, 26 Jul 2005 11:35:33 -0500
Subject: [Microscopy] Re: viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Anita

Also, contact Bill Miller at microbill-at-mohawk.net or by phone at (860)672-0068.

Just a reminder: remember that what makes a stereo microscope stereo is your looking with two eyes and that a camera will only "look" with one. You may need to adjust the lighting to make your objects look a little more 3D.

Good hunting!

Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Fall? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.

At 10:59 AM 7/26/2005, mccaulak-at-wfu.edu wrote:


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From: edelmare-at-muohio.edu
Date: Tue, 26 Jul 2005 11:45:32 -0500
Subject: [Microscopy] Re: viaWWW: video camera for stereomicroscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anita:

"Consumer-grade" cameras these have built in non-removable
lenses. Unless you are really straped for money you want a camera
which makes use of the steroscope's optics (for which you spent
alot more than the $0.5-$10 piece of plastic sitting on a consumer
grade camera). In which case you are now looking for a C-mount
camera.

Since you have the SZX-12 I would defintiely recommend
getting a U-TV0.5XC-2; CCD CAMERA ADAPTER, for mounting
a c-mount "video camera". It is very compact, and has built in
parfocalizing optics (for matching the eye pieces to the camera
view) which you set and forget. (You will have to determine if you
need to add other compoonents to get a photo port on your SZX12.
If you must go through the eye-piece for cost reasons there are
some very nice video couplers for this. E.g. Thales Optem
optemintl.com or Diagnostic Instruments www.diaginc.com )

Next up for "video camera" becomes what do you want to do
with it? What do you mean by "video camera"? Do you truely mean
"video" as in television, VCR's, "live" motion video?

Standard NTSC (north american television) show you can
display it on a "TV monitor", or do you want to use a computer to
capture images? A tv monitor will require an analog input (or DV
input), and you will need a capture card (Grabber) or a digital
camera output (DV or IEEE-1394/firewire, etc.) to convert this to
something a computer can use.

Still images or motion analysis? Still images moves into "digtal
cameras" but motion anaylsis starts dealing with "frame" rates (for
normal TV-monitors you need Standard NTSC = 30 frames/sec
and PAL 25 frames/sec - yes, interlaced).

Monochrome or color?

Then you get into resolution standard NTSC is 640x480pixels
good and inexpensive for TV monitors but not very good for
"publication". Higher resolution 1024 x 768 or 1280 x 980 or HDTV

Low light (fluorescence) vs normal bright-field / reflected light
work?

Hmm, looking around I found www.theimagingsource.com and
it has lots of c-mount camera choices and pricing! (I have never
shoped there yet but it does look very interesting) There are lots of
other sources for "video" camera out there. and pricing will range
from $300 to $5,000 on up into pricing over $200,000. I am sure
you will here from a few vendors shortly.

In video cameras we here have a Dage MTI camera, two Sony
cameras, and a Javalin camera collected through the years (none
of which are available any longer)

Good luck and feel free to contact me back with more
questions.

Note: We're a univeristy EM Faciltiy I do not sell any video
equipment nor hold any financial interests in any company
mentioned above . . . at least I don't think I do.

On 26 Jul 2005, at 8:51, mccaulak-at-wfu.edu wrote:

} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: video camera for stereomicroscope
}
} Question: I am looking to add a video camera to an existing
} Olympus SZX12 stereomicroscope. I would like to consider cameras
} ranging from consumer-grade cameras bought at big-box electronic
} stores to those made for scientific applications. Currently, I am
} having trouble finding much information regarding scientific-grade
} video cameras.
}
} I would appreciate hearing from folks that have been using either
} type of camera on a stereomicroscope, the pros and cons to their
} set-ups, and whether they are happy with their configuration.
}
} Thanks for the help.
}
} ---------------------------------------------------------------------------


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"WE ARE MICROSOFT.
RESISTANCE IS FUTILE.
YOU WILL BE ASSIMILATED."

==============================Original Headers==============================
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From: donc-at-asmicro.com
Date: Tue, 26 Jul 2005 11:49:55 -0500
Subject: [Microscopy] Re: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debby Sherman wrote "If you have no private labs near you than you can
probably get away with the lower [prices]." I've always been concerned about
the fuzzy definition of "near-ness". My AFM analytical services business in
Indianapolis, Indiana has a worldwide clientele because express courier
services extend our reach. This is fortunate, for if I had to depend on the
business generated within the state of Indiana I would have gone out of
business many years ago.

George Langford comments that a survey of going rates might violate
antitrust laws. Nevertheless, I think the policy of government granting
agencies requires this. Remember: no one ever said the government has to
be self-consistent.

The unfortunate aspect of university services is that they really are
driving the commercial labs out of business. I can say with confidence that
the number of commercial, for-profit labs providing AFM service in the US
has declined significantly in the last few years. I know this because I
have been buying their AFM equipment for resale.

regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]

----- Original Message -----
From: dsherman-at-purdue.edu
To: donc-at-asmicro.com
Sent: Tuesday, July 26, 2005 11:11 AM
Subject: [a] [Microscopy] Re: viaWWW: user charges for SEM and related





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Anita,

You do have to be careful about this topic on the list. There is a
concern
(rightly so) from for-profit companies that Universities will deprive them
of business by undercutting fees. The question you ask is fairly complex.
You do need to look at all costs associated with your facility (service
contracts, consumables, telephone, etc) and come up with a rate structure
that would cover these costs if you needed to charge all current users.

Then look at your internal users as subsidized. To the base costs, add
the
percentage used by your university for indirect costs. This would be the
same amount charged to grants to cover building costs, lights, heating,
etc.
This can be as high as 50% in many institutions.

If you are doing the work than figure your salary, fringe benefits, etc.
to
get an hourly rate that compensates totally for your time.
Tack on a few extra dollars when figuring consumable costs as prices go up
regularly and you need to recoup these costs without having to change your
whole rate structure.

For example: Assume an SEM with $15,000 service contract and consumables
(filaments, nitrogen, etc) of $2000/yr. Figure 1000 hours of billable use
time results in an hourly charge of $17/hr. Add time you spend
maintaining
the instrument (aligning, changing filaments, other non-billable time
adding
up to 1000hrs/yr) and this figure can easily double. Add the 50% overhead
and you arrive at $51/hr.

This amount is very low compared to what for-profit labs must charge as
they
have to use capital to purchase the instrument in the first place and then
depreciate it so they will have funds to replace when necessary. They
also
need to pay all costs associated with the physical structures,
advertising,
etc. This is why they are concerned about Universities undercutting their
necessary charges. If you have no private labs near you than you can
probably get away with the lower costs. Industry would be delighted with
this amount/hr as their costs if they owned the machine would be much
greater due to the depreciation, etc.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



On 7/26/05 8:52 AM, "mccaulak-at-wfu.edu" {mccaulak-at-wfu.edu} wrote:

}
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} Below is the result of your feedback form (NJZFM-ultra-55). It was
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} by (mccaulak-at-wfu.edu) from http://www.microscopy.com/MLFormMail.html on
} Monday, July 25, 2005 at 12:09:42


} --------------------------------------------------------------------------
-
}
} Email: mccaulak-at-wfu.edu
} Name: Anita McCauley
}
} Organization: Wake Forest University
}
} Title-Subject: [Filtered] MListserver: user charges for SEM and related
} equipment
}
} Question: I run a small microscopy facility in which equipment is
normally
} made available to users free of charge. We are currently developing a
} relationship with an outside user and would like to generate a usage fee
list
} so that we can recover costs for equipment wear and consumables.
}
} Rather than pulling numbers out of thin air, I would like to know what
others
} charge for the use of an SEM, a critical point dryer, and a sputter
coater.
} Tech time does not need to be included. Hopefully with this
information, I
} will be able to put together a reasonable usage fee list.
}
} Thanks.
}


} --------------------------------------------------------------------------
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13, 21 -- From dsherman-at-purdue.edu Tue Jul 26 11:07:59 2005
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related
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==============================Original Headers==============================
35, 21 -- From donc-at-asmicro.com Tue Jul 26 11:49:55 2005
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35, 21 -- References: {200507261611.j6QGBI4o006065-at-ns.microscopy.com}
35, 21 -- Subject: Re: [Microscopy] user charges for SEM and related
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From: beth-at-plantbio.uga.edu
Date: Tue, 26 Jul 2005 11:51:32 -0500
Subject: [Microscopy] sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I apologize to the list for posting Marilyn vos Savant's statement on
microscopy. I just wanted to share the article because I thought it was
humorous...I shall refrain from such postings.
best,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



==============================Original Headers==============================
8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26 11:51:32 2005
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==============================End of - Headers==============================




From: pgrover-at-bilbo.bio.purdue.edu
Date: Tue, 26 Jul 2005 11:54:45 -0500
Subject: [Microscopy] Re: user charges for SEM and related equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

George and Debby are correct. It is not fair to independent (=non taxpayer
subsidized) labs when a university facility charges artificially low fees
for instruments bought with public money, and it is actually a violation of
federal law for them to compete in this manner. I remember that in the '70s
Okla. State University's 'dairy barn' had to stop selling dairy products at
prices below those of local grocery stores. However more recently I
couldn't get business for my own SEM services because our Lafayette, IN
industries tell me they can get their work done at our local university for
little or no cost. But might as well mention the illegality of this
practice to a brick wall. Grrrrrrrr.

Paul Grover
Erstwhile Chief Microscopist & Bottle Washer
Microvista Laboratory


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln




==============================Original Headers==============================
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6, 24 -- Subject: [Microscopy] Re: user charges for SEM and related equipment
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From: hoffpajo-at-yahoo.com
Date: Tue, 26 Jul 2005 12:05:04 -0500
Subject: [Microscopy] Re: sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

eh go ahead and post, some of us got a chuckle out of
it

--- beth-at-plantbio.uga.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
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}
} I apologize to the list for posting Marilyn vos
} Savant's statement on
} microscopy. I just wanted to share the article
} because I thought it was
} humorous...I shall refrain from such postings.
} best,
} Beth
}
}
**********************************************************************
} Beth Richardson
} EM Lab Coordinator
} Plant Biology Department
} University of Georgia
} Athens, GA 30602-7271
}
} Phone - (706) 542-1790 & FAX - (706) 542-1805
} http://www.plantbio.uga.edu/emlab
}
} "Between the two evils,
} I always pick the one I never tried before". Mae
} West (1893-1980)
}
*******************************************************************
}
} "And it's only the giving that makes you what you
} are".
} Wond'ring Aloud, Jethro Tull (Aqualung)
}
}
************************************************************************
}
} ***
}
}
}
} ==============================Original
} Headers==============================
} 8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26
} 11:51:32 2005
} 8, 18 -- Received: from dogwood.plantbio.uga.edu
} (dogwood.plantbio.uga.edu [128.192.26.2])
} 8, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id j6QGpVhb000423
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} Jul 2005 11:51:31 -0500
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} DES-CBC3-SHA (168 bits))
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} 8, 18 -- From: Beth Richardson
} {beth-at-plantbio.uga.edu}
} 8, 18 -- To: microscopy {microscopy-at-microscopy.com}
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} ==============================End of -
} Headers==============================
}




____________________________________________________
Start your day with Yahoo! - make it your home page
http://www.yahoo.com/r/hs


==============================Original Headers==============================
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From: dmclea-at-sandia.gov
Date: Tue, 26 Jul 2005 12:08:09 -0500
Subject: [Microscopy] sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Beth,

We must remember, that Microscopists do not live by electrons alone...a
little levity is ALWAYS WELCOME! Especially at the National Labs!

Dorrance McLean
Sandia National Laboratories
Livermore, CA

-----Original Message-----
X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu]
Sent: Tuesday, July 26, 2005 9:52 AM
To: McLean, Dorrance

I apologize to the list for posting Marilyn vos Savant's statement on
microscopy. I just wanted to share the article because I thought it was
humorous...I shall refrain from such postings.
best,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



==============================Original
Headers==============================
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==============================Original Headers==============================
19, 29 -- From dmclea-at-sandia.gov Tue Jul 26 12:08:09 2005
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From: gwe-at-ufl.edu
Date: Tue, 26 Jul 2005 12:08:12 -0500
Subject: [Microscopy] M&M 2005 Attendees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If you have any announcements to be included in the daily newsleter of
the meeting, please email me before noon (EST) on Friday or drop your
copy by the LAC Headquarters at the convention center.

Thanks,
Greg

--
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
Phone: 352-392-1295
Fax: 352 846 0251



==============================Original Headers==============================
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From: granta-at-geol.queensu.ca
Date: Tue, 26 Jul 2005 12:19:39 -0500
Subject: [Microscopy] Re: sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Don't apologize - it was a humorous (and brief) posting. (I noticed the
complainers were mostly men- significant??).
Keep 'em coming!

Alan Grant
Dept. of Geological Sciences and Geological Engineering
Queen's University
Kingston ON
Canada K7L 3N6


beth-at-plantbio.uga.edu wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
5, 19 -- From granta-at-geol.queensu.ca Tue Jul 26 12:19:39 2005
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5, 19 -- From: Alan Grant {granta-at-geol.queensu.ca}
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From: hoffpajo-at-yahoo.com
Date: Tue, 26 Jul 2005 12:24:27 -0500
Subject: [Microscopy] sorry about that

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i didn't complain just pointed out a few things that
were left out.

--- granta-at-geol.queensu.ca wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
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}
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}
} Don't apologize - it was a humorous (and brief)
} posting. (I noticed the
} complainers were mostly men- significant??).
} Keep 'em coming!
}
} Alan Grant
} Dept. of Geological Sciences and Geological
} Engineering
} Queen's University
} Kingston ON
} Canada K7L 3N6
}
}
} beth-at-plantbio.uga.edu wrote:
}
}
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} ----------------------------------------------------------------------------
} }
} } I apologize to the list for posting Marilyn vos
} Savant's statement on
} } microscopy. I just wanted to share the article
} because I thought it was
} } humorous...I shall refrain from such postings.
} } best,
} } Beth
} }
}
} **********************************************************************
} } Beth Richardson
} } EM Lab Coordinator
} } Plant Biology Department
} } University of Georgia
} } Athens, GA 30602-7271
} }
} } Phone - (706) 542-1790 & FAX - (706) 542-1805
} } http://www.plantbio.uga.edu/emlab
} }
} } "Between the two evils,
} } I always pick the one I never tried before". Mae
} West (1893-1980)
}
} *******************************************************************
} }
} } "And it's only the giving that makes you what you
} are".
} } Wond'ring Aloud, Jethro Tull (Aqualung)
} }
}
} ************************************************************************
}
} } ***
} }
} }
} }
} } ==============================Original
} Headers==============================
} } 8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26
} 11:51:32 2005
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} 5, 19 -- From granta-at-geol.queensu.ca Tue Jul 26
} 12:19:39 2005
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____________________________________________________
Start your day with Yahoo! - make it your home page
http://www.yahoo.com/r/hs


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From: maureen_petersen-at-msn.com
Date: Tue, 26 Jul 2005 15:35:38 -0500
Subject: [Microscopy] RE: sorry about that

Contents Retrieved from Microscopy Listserver Archives
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Beth: I noticed that a critical comment about this (took too much of his
time?) took the time to post two times on the topic. I think some people
take themselves too seriously.

Maureen Petersen
Duke Univ.

} From: beth-at-plantbio.uga.edu
} Reply-To: microscopy-at-microscopy.com
} To: Maureen_Petersen-at-msn.com
} Subject: [Microscopy] sorry about that
} Date: Tue, 26 Jul 2005 11:52:00 -0500
}
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From: sryazant-at-ucla.edu
Date: Tue, 26 Jul 2005 15:35:54 -0500
Subject: [Microscopy] Re: sorry about that

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Don't be sorry: it was good posting and we need something humorous here
from time to time. Sergey

At 09:53 AM 7/26/2005, you wrote:



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From: chiphead-at-sbcglobal.net
Date: Tue, 26 Jul 2005 15:58:51 -0500
Subject: [Microscopy] Re: Identifications, Courtesy etc

Contents Retrieved from Microscopy Listserver Archives
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And I wouldn't take much (any) notice of postings by anyone who doesn't identify
themselves but who hides behind a freebie email address

cheers

rtch



Date sent: Tue, 26 Jul 2005 15:37:50 -0500
To: r.sims-at-auckland.ac.nz
X-from: maureen_petersen-at-msn.com
Send reply to: microscopy-at-microscopy.com

--- r.sims-at-auckland.ac.nz wrote:
} And I wouldn't take much (any) notice of postings by
} anyone who doesn't identify
} themselves but who hides behind a freebie email
} address
}
} cheers
}
} rtch

If this were a totally "private" list, I would agree
with you. But given that it is realatively open, and
given the current climate, from a corporate, identity
theft, legal "this opinion is not necess....", etc., I
don't know that it's fair to just write off a poster
because they choose not to be fully identified.

John W. Raffensperger, Jr.
Beaver Dam, Wisconsin, USofA


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From: avklaus-at-amnh.org
Date: Tue, 26 Jul 2005 16:23:40 -0500
Subject: [Microscopy] sorry about that

Contents Retrieved from Microscopy Listserver Archives
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Ditto. It was funny and appreciated. I shared it with several friends.
Thanks for posting. -Angela

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} Don't be sorry: it was good posting and we need something humorous here
} from time to time. Sergey
}
} At 09:53 AM 7/26/2005, you wrote:
}
}
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} } America


--
Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA
Email: avklaus-at-amnh.org
Tel: 212-796-5977
Fax: 212-496-3480


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From: zaluzec-at-microscopy.com
Date: Tue, 26 Jul 2005 17:49:32 -0500
Subject: [Microscopy] Administrivia: sorry about that/most bright people

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Beth & everyone else

There was no problem with Beth's initial posting and no apology
was needed here Beth. Sorry if I appeared to have come down on you.

The followups IMHO were starting to drift into critiques of Marilyn whomever
and her "crap Journalism" and I was trying to nip that direction of
critique in the bud. However, I see I had the opposite effect. Oh well.....

As Dorrance said, everyone can use a grin occassionally .


Nestor






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From: sryazant-at-ucla.edu
Date: Tue, 26 Jul 2005 18:40:35 -0500
Subject: [Microscopy] Re: Administrivia: sorry about that/most bright

Contents Retrieved from Microscopy Listserver Archives
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Nestor
I don't see any reason why we could not discuss the quality of somebody's
work even if it's a journalist with former (?) high IQ. Is it prohibited
to discuss people's work with high IQ on this ListServer? Sergey

At 03:51 PM 7/26/2005, you wrote:



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_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
and UCLA Institute of Nanotechnology
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095


Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu




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From: cornheadorama-at-hotmail.com
Date: Tue, 26 Jul 2005 18:46:47 -0500
Subject: [Microscopy] viaWWW: Looking for limited TAS(+?) motor wiring information

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, July 26, 2005 at 18:41:08
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: Techutopia

Title-Subject: [Filtered] Looking for limited TAS(+?) motor wiring information.

Question: Hi,

I have a Leitz Orthoplan microscope from a TAS (or is it TAS+?) system. It has motorized stage and fine focus, relay lenses etc. for video.

I didn't pick up the console, controller, or schematics. I'm getting pretty handy with LabVIEW machine vision and motion control, so I'd like to revive this scope around a PC platform.

I need limited wiring diagram information. In particular, I need pinouts for the stepper motors, or to figure out the pinouts indirectly from other elements of the schematic. Ratings or replacement part information for the motors might be helpfull too, barring that I may be able to approximate these by studying the schematic in more detail.

If you have any wiring information for these things, please let me know.

} From the number of pins present, it appears as though the X and Y drives may have encoders associated with them, wheras focus deson't. I don't have the image rotation prism option, and in fact I'd be passively interested in buying one (image roatation is pretty trivial in SW these days).

I'd also be willing to part with the 'scope as opposed to undertaking the project if you're a big fan, and if I do proceed with the PC retrofit I may be offering the whole package after I've played with it for a while. The real motivation for having it evaporated soon after I bought it several years ago.

I can be reached at cornheadorama-at-hotmail.com

-Jeff

---------------------------------------------------------------------------

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From: zaluzec-at-microscopy.com
Date: Tue, 26 Jul 2005 19:09:12 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Person

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sergey

A honest discussion or even disagreement about a subject or work which is microscopy
related (even tangentially) is not prohibited.

However, it is against the rules to intentionally disparage any person
or organization on the Listserver. This rule applies equally to Journalists as well
as Microscopists. Please review the rules which you received upon subscription.


http://microscopy.com/MicroscopyListserver/Rules.html

Presumably a person of hi IQ has the ability to distinguish between these two.

Nestor
Your Friendly Neighborhood SysOp







} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
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15, 13 -- To: microscopy-at-microscopy.com
15, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
15, 13 -- Subject: Discussion of high IQ vs Disparagement of a Person
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From: sryazant-at-ucla.edu
Date: Tue, 26 Jul 2005 20:26:30 -0500
Subject: [Microscopy] Re: Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My IQ is very low, Nestor, so I am electron microscopist for last 30
years... Is against rules to say truth? You know, truth sometime hurts
and may be politically incorrect... This forum becomes too much politically
correct and heavily censured (yes, it's true), so I am loosing interest
here... I had enough censorship and "rules" in USSR. But, I have to admit
US is over performing USSR now, so I feel I am at home. Sergey


At 05:10 PM 7/26/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
and UCLA Institute of Nanotechnology
10833 Le Conte Ave, Room 33-080
Los Angeles, CA 90095


Phone: (310) 825-1144 (office)
(310) 206-1029 (Lab)
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu




==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Tue, 26 Jul 2005 20:30:52 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HERE HERE well said

--- sryazant-at-ucla.edu wrote:

}
}
}
}
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} The Microscopy ListServer -- CoSponsor: The
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}
} My IQ is very low, Nestor, so I am electron
} microscopist for last 30
} years... Is against rules to say truth? You know,
} truth sometime hurts
} and may be politically incorrect... This forum
} becomes too much politically
} correct and heavily censured (yes, it's true), so I
} am loosing interest
} here... I had enough censorship and "rules" in
} USSR. But, I have to admit
} US is over performing USSR now, so I feel I am at
} home. Sergey
}
}
} At 05:10 PM 7/26/2005, you wrote:
}
}
}
}
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
} ----------------------------------------------------------------------------
} }
} } Sergey
} }
} } A honest discussion or even disagreement about a
} subject or work which
} } is microscopy
} } related (even tangentially) is not prohibited.
} }
} } However, it is against the rules to intentionally
} disparage any person
} } or organization on the Listserver. This rule
} applies equally to
} } Journalists as well
} } as Microscopists. Please review the rules which
} you received
} } upon subscription.
} }
} }
}
} http://microscopy.com/MicroscopyListserver/Rules.html
} }
} } Presumably a person of hi IQ has the ability to
} distinguish between these
} } two.
} }
} } Nestor
} } Your Friendly Neighborhood SysOp
} }
} }
} }
} }
} }
} }
} }
} }
}
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
} ----------------------------------------------------------------------------
} } }
} } } Nestor
} } } I don't see any reason why we could not discuss
} the quality of somebody's
} } } work even if it's a journalist with former (?)
} high IQ. Is it prohibited
} } } to discuss people's work with high IQ on this
} ListServer? Sergey
} } }
} } } At 03:51 PM 7/26/2005, you wrote:
} } }
} } }
} } }
} }
}
} } ------------------------------------------------------------------------
}
} } ----
} } } } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
} } ------------------------------------------------------------------------
}
} } ----
} } } }
} } } } Beth & everyone else
} } } }
} } } } There was no problem with Beth's initial
} posting and no apology
} } } } was needed here Beth. Sorry if I appeared to
} have come down on you.
} } } }
} } } } The followups IMHO were starting to drift into
} critiques of Marilyn
} } whomever
} } } } and her "crap Journalism" and I was trying to
} nip that direction of
} } } } critique in the bud. However, I see I had the
} opposite effect. Oh well.....
} } } }
} } } } As Dorrance said, everyone can use a grin
} occassionally .
} } } }
} } } }
} } } } Nestor
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
}
} ----------------------------------------------------------------------
}
} } ------
} } } } } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of
} } America
} } } } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } } } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
}
} ----------------------------------------------------------------------
}
} } ------
} } } } }
} } } } } I apologize to the list for posting Marilyn
} vos Savant's statement on
} } } } } microscopy. I just wanted to share the article
} because I thought it was
} } } } } humorous...I shall refrain from such postings.
} } } } } best,
} } } } } Beth
} } } } }
} } } }
}
} **********************************************************************
} } } } } Beth Richardson
} } } } } EM Lab Coordinator
} } } } } Plant Biology Department
} } } } } University of Georgia
} } } } } Athens, GA 30602-7271
} } } } }
} } } } } Phone - (706) 542-1790 & FAX - (706)
} 542-1805
} } } } } http://www.plantbio.uga.edu/emlab
} } } } }
} } } } } "Between the two evils,
} } } } } I always pick the one I never tried before".
} Mae West (1893-1980)
} } } }
}
} *******************************************************************
} } } } }
} } } } } "And it's only the giving that makes you what
} you are".
} } } } } Wond'ring Aloud, Jethro Tull (Aqualung)
} } } } }
} } } }
}
} ************************************************************************
} } } } } ***
} } } } }
} } } } }
} } } } }
} } } } } ==============================Original
} } Headers==============================
} } } } } 8, 18 -- From beth-at-plantbio.uga.edu Tue Jul 26
} 11:51:32 2005
} } } } } 8, 18 -- Received: from
} dogwood.plantbio.uga.edu
} } } } (dogwood.plantbio.uga.edu [128.192.26.2])
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} (8.12.11/8.12.8) with ESMTP id
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} {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005
} } } } 11:51:31 -0500
} } } } } 8, 18 -- Received: from localhost
} ([127.0.0.1])
}
=== message truncated ===




____________________________________________________
Start your day with Yahoo! - make it your home page
http://www.yahoo.com/r/hs


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From: cgarber-at-2spi.com
Date: Tue, 26 Jul 2005 21:51:09 -0500
Subject: [Microscopy] Apology to the list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Great posting. One has to wonder about the role model of ethical values when
one bases their decisions on the basis of what they can "get away " with.

You probably think I am losing my balls for keeping silent. I almost
composed a posting but then again, I did not want to antagonize some of my
best customers. Which is of course exactly what I woudl be doing.

Maybe I will have some scotch and get up the nerve to create a posting.
Question: Will we see you in Honolulu?

Chuck
----- Original Message -----
X-from: {donc-at-asmicro.com}
To: {cgarber-at-2spi.com}
Sent: Tuesday, July 26, 2005 12:52 PM

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Please accept my apologies for inadvertently sending out a message that was
going to an individual and was obviously not intended for the list at large.

I am embarrassed about this and will try to make sure this never happens
again.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




==============================Original Headers==============================
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From: zaluzec-at-microscopy.com
Date: Tue, 26 Jul 2005 22:02:16 -0500
Subject: [Microscopy] Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's OK Chuck, Scotch can do this to you! ;-)
Markus
----- Original Message -----
X-from: {cgarber-at-2spi.com}
To: {micro-at-superlink.net}
Sent: Tuesday, July 26, 2005 10:51 PM

Sergy

I don't care who you are or where your from, but I insist the rules which have
been established for over a decade are followed by all. Since you
believe you are above the rules so be it. You are welcome to
go elsewhere.

I have canceled your subscription to the Listserver.


Nestor
Microscopy SysOp



==============================Original Headers==============================
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From: tina-at-pbrc.hawaii.edu
Date: Tue, 26 Jul 2005 23:31:26 -0500
Subject: [Microscopy] Daily newsletter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Greg-

Are you thinking of having a Saturday newsletter? There was one in
Savannah... I don't critical.

My cell phone number is 808-428-7546

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


==============================Original Headers==============================
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From: tina-at-pbrc.hawaii.edu
Date: Tue, 26 Jul 2005 23:37:38 -0500
Subject: [Microscopy] Sorry for mass post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yikes! I laugh when people inadvertantly post to the entire List, and now
I've done it! Sorry! And missing a couple of words, too. I'm tired...

See many of you in Honolulu next week!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



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From: schooley-at-mcn.org
Date: Tue, 26 Jul 2005 23:47:57 -0500
Subject: [Microscopy] Re: Sorry for mass post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Yikes! I laugh when people inadvertantly post to the entire List, and now
} I've done it! Sorry! And missing a couple of words, too. I'm tired...

And now everyone has your cell #...

CS
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

==============================Original Headers==============================
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From: as-at-astonmet.com
Date: Wed, 27 Jul 2005 07:41:31 -0500
Subject: [Microscopy] Re: Apology to the list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chuck,

No apology needed. There are laws and ethics that we overtly or tacitly
agree to abide by. If there is language in a contract, regulation or law
then we would all like to believe that our peers actually abide by those
terms.

We all have financial issues to contend with and I would hope our community
conducts it business/research/education with respect and fairness to
others. If an NSF grant or monies require directing a commercial service
AFM inquiry to a service lab in Indiana, then it should be done without
question. It is a moral and legal obligation. No excuses just as that
Indiana lab is not excused from paying taxes which in turn supports those
grants and institutions.

Alan Stone


At 09:52 PM 7/26/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com


==============================Original Headers==============================
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From: phillipst-at-missouri.edu
Date: Wed, 27 Jul 2005 08:14:54 -0500
Subject: [Microscopy] Re: Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor: I would respectfully ask you to reconsider dropping Sergey from the
list. His comment was not that egrious. He is a very active member of this
listserver and it would be a shame to drop him. I have never met him and
this comment is not motivated by friendship or a personal tie. Tom Phillips

At 10:03 PM 07/26/05, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



==============================Original Headers==============================
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From: eschumacher-at-mccrone.com
Date: Wed, 27 Jul 2005 08:17:39 -0500
Subject: [Microscopy] Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor,

I was frankly relieved to see you step in a couple of times on this one,
and your reminder to everyone to review the rules is obviously
necessary. (I had already pulled them out myself yesterday, as a check
against some of what was being posted.)

I was surprised by the tone and the subjectivity of some of the initial
responses to what was obviously an amusing posting, and people do need
to be reminded that the listserver is an educational/informational
exchange, rather than a personal soapbox. I don't think it's an
accident that the words, 'not a right, but a privilege', show up more
than once in the rules.

Keep up the good work!

See you next week,

Elaine

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************

-----Original Message-----
X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
Sent: Tuesday, July 26, 2005 10:03 PM
To: Elaine F. Schumacher

Sergy

I don't care who you are or where your from, but I insist the rules
which have
been established for over a decade are followed by all. Since you
believe you are above the rules so be it. You are welcome to
go elsewhere.

I have canceled your subscription to the Listserver.


Nestor
Microscopy SysOp



==============================Original
Headers==============================
7, 11 -- From zaluzec-at-microscopy.com Tue Jul 26 22:02:16 2005
7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
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7, 11 -- To: microscopy-at-microscopy.com
7, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
7, 11 -- Subject: Administrivia: Rules
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==============================Original Headers==============================
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From: zaluzec-at-microscopy.com
Date: Wed, 27 Jul 2005 08:35:03 -0500
Subject: [Microscopy] Administrivia: Posting to the List vs Replying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

I've gotten several questions lately on why things are operating slighlty differently recently.
Particuliarly with respect to replies. If your interested please read on otherwise
just trash this.

As you know SPAM has grown enormously this list gets "attacked" daily. The
rate is approaching 1000 spam messages per day. Most of these were stopped but
about one or two every week managed to get through.

What you MAY NOT know is that every message that is rejected by the listserver
software gets a reply sent to the originator, letting them know there was a problem
and how to deal with it. A number of you occassional get this message when there
a problem with things you try to post. However on average this is a low number.

What you DEFINITELY don't know is that alot of the SPAM addresses are bogus and various
Email servers just return the "There is a problem with your Email" message as undeliverable.
Now think for a minute these messages are returned to someone and guess who that is....

Yep I've get barraged by huge numbers of not only spam but also literally hundreds of
rejected mail messages the number of which grows daily. Being somewhat consciencous
I check each just in case it was a real subscriber that had a problem.

The long and short of it was that things were just getting unmanagable,
and I had to redesign the filter software. This has reduced the probem from 750+ junk
messages/day to 1 or 2. The unfortunate thing is that now every message
has a REPLY line which say microscopy listserver
and it has a FROM line indicating the originator of the Email.


The net result of this is if you REPLY the message goes to the list. If you want
to send a private message you will need to copy and paste the Email address
of the sender into a new Email message. At the moment that is the correct
procedure.

There are pro's and con's of this, the advantage is that it has made my life
alot less annoying when I get home in the evening and only have to deal with
a few problems. The Con, is the occassional, inadvertently posted message.

I'll pontificate abit on this abit longer and try to figure out a
solution to the problem, which is compatible with both my needs to occassionally
have a real life out side of this electronic world, and the convenience of
replying . To be honest, I have lots to do right now and this is not a simple
issue as there are lots of hidden loop holes which need to be managed.

For the moment just try to pay close attention. Whenever you hit reply
look at the "TO" address , the location where a message is going is displayed
on you Email and is never hidden by any good Email client program.


Nestor
Your Tired Friendly (?) Neighborhood SysOp

==============================Original Headers==============================
14, 11 -- From zaluzec-at-microscopy.com Wed Jul 27 08:35:03 2005
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14, 11 -- Date: Wed, 27 Jul 2005 08:35:02 -0500
14, 11 -- To: microscopy-at-microscopy.com
14, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
14, 11 -- Subject: Administrivia: Posting to the List vs Replying
14, 11 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: mcauliff-at-umdnj.edu
Date: Wed, 27 Jul 2005 08:43:16 -0500
Subject: [Microscopy] Re: Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you, Nestor. I really hope this thread will die now.

Geoff

zaluzec-at-microscopy.com wrote:

}
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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



==============================Original Headers==============================
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8, 31 -- microscopy-at-microscopy.com; Wed, 27 Jul 2005 09:40:20 -0400 (EDT)
8, 31 -- Date: Wed, 27 Jul 2005 09:40:19 -0400
8, 31 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
8, 31 -- Subject: Re: [Microscopy] Administrivia: Rules
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From: Jane.LaGoy-at-bodycote.com
Date: Wed, 27 Jul 2005 08:47:35 -0500
Subject: [Microscopy] my $0.02 worth on disparagement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was enjoying the IQ discourse when it was light-hearted, but I don't
believe the Microscopy Listserver, or any other professional listserver,
should disparage people personally. It is not a question of censorship but
of human decency. What is it our parents said? "If you can't say something
nice about someone then don't say anything at all". The IQ lady publicly
wrote her opinion, so that leaves those words as open game for criticism. I
think it is unfortunate that Sergey was not allowed to make his own choice
about leaving the list; in that way, he was censored. This is my personal
opinion (and since I submitted it, you folks are all welcome to criticize
it.)

Jane L. LaGoy
R&D Engineer
Bodycote HIP
155 River Street
Andover, MA 01810


==============================Original Headers==============================
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3, 15 -- From: JLaGoy {Jane.LaGoy-at-bodycote.com}
3, 15 -- To: "Microscopy Listserver (E-mail)" {Microscopy-at-MSA.Microscopy.com}
3, 15 -- Subject: my $0.02 worth on disparagement
3, 15 -- Date: Wed, 27 Jul 2005 09:48:34 -0400
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From: marc.pypaert-at-yale.edu
Date: Wed, 27 Jul 2005 09:36:28 -0500
Subject: [Microscopy] Administrivia: Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would back this opinion. Especially since the line:
"Presumably a person of hi IQ has the ability to distinguish between
these two"
in Nestor's email could have been misinterpreted by Sergey as an
unwarranted
and disparaging remark on his IQ level. At least, this is the way I
understood
it, and apparently Sergey too. I find this very disturbing...

Marc


On Wednesday, July 27, 2005, at 09:15 AM, phillipst-at-missouri.edu wrote:

}
}
}
} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------
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}
} Nestor: I would respectfully ask you to reconsider dropping Sergey
} from the
} list. His comment was not that egrious. He is a very active member of
} this
} listserver and it would be a shame to drop him. I have never met him
} and
} this comment is not motivated by friendship or a personal tie. Tom
} Phillips
}
} At 10:03 PM 07/26/05, you wrote:
}
}
}
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ------
} }
} } Sergy
} }
} } I don't care who you are or where your from, but I insist the rules
} } which have
} } been established for over a decade are followed by all. Since you
} } believe you are above the rules so be it. You are welcome to
} } go elsewhere.
} }
} } I have canceled your subscription to the Listserver.
} }
} }
} } Nestor
} } Microscopy SysOp
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 7, 11 -- From zaluzec-at-microscopy.com Tue Jul 26 22:02:16 2005
} } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com
} } [206.69.208.22])
} } 7, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
} } j6R32FsY019078
} } 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2005
} } 22:02:16
} } -0500
} } 7, 11 -- Mime-Version: 1.0
} } 7, 11 -- Message-Id: {p0611040cbf0ca908c2d4-at-[206.69.208.22]}
} } 7, 11 -- Date: Tue, 26 Jul 2005 22:02:14 -0500
} } 7, 11 -- To: microscopy-at-microscopy.com
} } 7, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} } 7, 11 -- Subject: Administrivia: Rules
} } 7, 11 -- Content-Type: text/plain; charset="us-ascii"
} } ==============================End of -
} } Headers==============================
}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 2 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
}
}
} ==============================Original
} Headers==============================
} 9, 20 -- From PhillipsT-at-missouri.edu Wed Jul 27 08:14:54 2005
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--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original Headers==============================
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From: bmollon-at-pacbell.net
Date: Wed, 27 Jul 2005 09:41:38 -0500
Subject: [Microscopy] rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor..........since you just used a derogatory and
demeaning statement about someone publically using the
listserver........are you going to remove yourself
from the list? I think the "I dont care who you are"
could have been left off and your point still made.

Nestor wrote:
"Sergy

I don't care who you are or where your from, but I
insist the rules
which have
been established for over a decade are followed by
all. Since you
believe you are above the rules so be it. You are
welcome to
go elsewhere.

I have canceled your subscription to the Listserver.


Nestor
Microscopy SysOp"



==============================Original Headers==============================
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From: john.vetrano-at-pnl.gov
Date: Wed, 27 Jul 2005 10:37:10 -0500
Subject: [Microscopy] Thanks, Nestor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You do so much for this community and use such an even hand and light touch
on this list that I could not believe that he was pushing it (and not for
the first time)! You have my full support. I'd post this but I don't want
to start/continue a flood!

Cheers, John


On 7/26/05 8:03 PM, "zaluzec-at-microscopy.com" {zaluzec-at-microscopy.com} wrote:

}
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}
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} Sergy
}
} I don't care who you are or where your from, but I insist the rules which have
} been established for over a decade are followed by all. Since you
} believe you are above the rules so be it. You are welcome to
} go elsewhere.
}
} I have canceled your subscription to the Listserver.
}
}
} Nestor
} Microscopy SysOp
}
}
}
} ==============================Original Headers==============================
} 7, 11 -- From zaluzec-at-microscopy.com Tue Jul 26 22:02:16 2005
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} 7, 11 -- To: microscopy-at-microscopy.com
} 7, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} 7, 11 -- Subject: Administrivia: Rules
} 7, 11 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of - Headers==============================

--
********
John S. Vetrano
Sr. Research Scientist
Materials Structure and Performance Group
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0724 Fax: (509)376-6308
Email: mailto:john.vetrano-at-pnl.gov


==============================Original Headers==============================
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7, 28 -- From: "Vetrano, John S" {john.vetrano-at-pnl.gov}
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From: Winston.Wiggins-at-cshs.org
Date: Wed, 27 Jul 2005 10:46:49 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Person

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

List members et al.,
Is there any way we can "restore" the system to the point before the I.Q.
posting, thereby returning Sergey to the List? I would hate to miss his
often insightful comments. I also hate to think that any comment, understood
or misunderstood, made in the heat of passion may summarily cause expulsion
sans appeal, deservedly or not.
"Can we all just get along?!"
Winston

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, E.M. Pathology Asst.
CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
Electron Microscopy, LLSPT, A823-A828
8700 Beverly Blvd.
Los Angeles, CA 90048
310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
Winston.Wiggins-at-CSHS.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

-----Original Message-----
X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
Sent: Tuesday, July 26, 2005 5:12 PM
To: Winston.Wiggins-at-CSHS.org

Sergey

A honest discussion or even disagreement about a subject or work which is
microscopy
related (even tangentially) is not prohibited.

However, it is against the rules to intentionally disparage any person
or organization on the Listserver. This rule applies equally to
Journalists as well
as Microscopists. Please review the rules which you received upon
subscription.


http://microscopy.com/MicroscopyListserver/Rules.html

Presumably a person of hi IQ has the ability to distinguish between these
two.

Nestor
Your Friendly Neighborhood SysOp

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==============================Original Headers==============================
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18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org}
18, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
18, 25 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of a Pers
18, 25 -- on
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From: tonygr-at-MIT.EDU
Date: Wed, 27 Jul 2005 11:30:48 -0500
Subject: [Microscopy] Re: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I tend to agree with Winston. This list is too valuable to be torn
asunder by one topic and a few heightened emotions. Perhaps when many
of us unsubscribe while attending M&M 2005 we can "reboot".


Joseph

Joseph M. Oparowski
Center for Materials Science - Consulting and Failure Analysis
Bose Corporation Joseph_Oparowski-at-bose.com
The Mountain, M/S 415 Phone: (508) 766-1371
Framingham, MA 01701-9168 Fax: (508) 766-1313



-----Original Message-----
X-from: Winston.Wiggins-at-cshs.org [mailto:Winston.Wiggins-at-cshs.org]
Sent: Wednesday, July 27, 2005 11:50 AM
To: Oparowski, Joseph

List members et al.,
Is there any way we can "restore" the system to the point before the
I.Q. posting, thereby returning Sergey to the List? I would hate to miss
his often insightful comments. I also hate to think that any comment,
understood or misunderstood, made in the heat of passion may summarily
cause expulsion sans appeal, deservedly or not.
"Can we all just get along?!"
Winston

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, E.M. Pathology Asst.
CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
Electron Microscopy, LLSPT, A823-A828
8700 Beverly Blvd.
Los Angeles, CA 90048
310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
Winston.Wiggins-at-CSHS.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

-----Original Message-----
X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
Sent: Tuesday, July 26, 2005 5:12 PM
To: Winston.Wiggins-at-CSHS.org

Sergey

A honest discussion or even disagreement about a subject or work which
is microscopy related (even tangentially) is not prohibited.

However, it is against the rules to intentionally disparage any person
or organization on the Listserver. This rule applies equally to
Journalists as well as Microscopists. Please review the rules which
you received upon
subscription.


http://microscopy.com/MicroscopyListserver/Rules.html

Presumably a person of hi IQ has the ability to distinguish between
these two.

Nestor
Your Friendly Neighborhood SysOp

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If you have received this message in error, please notify us
immediately, by calling (310) 423-6428 -- and destroy the related
message. Thank You for your cooperation.



==============================Original
Headers==============================
18, 25 -- From winston.wiggins-at-cshs.org Wed Jul 27 10:46:49 2005 18, 25
-- Received: from csip1.csmc.edu (CSIP1.csmc.edu [192.231.133.35])
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{B5AEAEDBB5D44C47BFE2A6D791329D7F2ADC7D-at-EXCHANGE24.csmc.edu}
18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org} 18, 25 --
To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 18, 25 --

I have to say I was waiting for Chuck's response on this thread - my jaw
dropped when I read it, until the explanation came in the next e-mail!!!

More seriously--

Most of the discussion on this thread has been about the harm done to
commercial labs by university labs "undercutting", "poaching customers" -
or whatever description you want to apply, and by the need to determine the
"true" cost of providing the service. I think, though, that there is a
serious problem for the university labs themselves in allowing commercial
service activities to become an important part of their operation.

Any operation has a core mission. The proprietor of any successful
business knows the importance of identifying and maintaining their core
mission, and the ex-proprietors of many failed businesses know the costs of
straying from the mission. While individual organizations vary, the core
mission of a university characterization laboratory is not the same as that
of a commercial laboratory. In a university our basic mission is education
through the generation of research results; that of the company is to
generate a profit through providing an analytical service. These
differences, though in some areas subtle, lead to totally different
approaches to the way we interact with our clients, the way we manage our
instrumentation, our time, etc, etc.

The government (through the NSF, in my case) contributes towards the cost
of setting up and maintaining my characterization facilities. If we
generate first-class results on new and interesting materials, then this is
considered money well-spent. If I dilute this by taking on commercial
work, even if this leads to a reduction in the amount of "subsidy", I am
reducing the rationale for getting any support, and in general diluting the
reputation of my laboratories.

I realize that not everyone in an academic environment has my good fortune
to work for an administration that supports the ideas I have just
expressed, and I also stress again that we each work in unique
environments, but in general I would suggest that in most cases commercial
clients are best left to commercial laboratories. A commercial client is
generally looking for an analytical service, rather than to be educated.

Tony Garratt-Reed.


At 10:46 PM 7/26/2005, you wrote:



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***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
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USA

Tel: 617-253-4622
Fax: 617-258-6478
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From: uti-at-direcpc.com
Date: Wed, 27 Jul 2005 11:32:22 -0500
Subject: [Microscopy] Re: Discussion of high IQ vs Disparagement of a Pers

Contents Retrieved from Microscopy Listserver Archives
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I agree, we need to re-boot!
It is great place and Sergey's knowledge is needed for us.
Thanks,
Vlad


At 11:24 AM 7/27/2005 -0500, you wrote:



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From: frank.karl-at-degussa.com
Date: Wed, 27 Jul 2005 11:46:03 -0500
Subject: [Microscopy] golden rule applies here

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Folks, lets remenber the Golden Rule with our dealing with the list
server....

The man with the gold get to make the rules. Simple is it not?

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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From: mochs-at-gwdg.de
Date: Wed, 27 Jul 2005 11:54:31 -0500
Subject: [Microscopy] Course announcement: Workshop on recent stereology

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Dear Colleagues,

Anybody wishing to integrate quantitative analysis by design-based
stereology into his/her studies might find this interesting. Please don´t
hesitate to contact me for further details.

Best regards,
Matthias



WORKSHOP ON RECENT STEREOLOGY - 19-23 September 2005,
Institute of Anatomy, University of Bern, Switzerland.

The workshop is intended for students and researchers from the field of
biology, medicine, and material sciences, interested in the theory and
practice of geometric sampling and measurement of 3D structures at the LM
and EM level.

The approach of the workshop is problem-based; ideally the participants
will bring questions and unsolved case studies, and the teachers will try
to supply concrete and detailed solutions, eventually explaining the
underlying theory in short ad-hoc lectures. No strong background in
mathematics or statistics is assumed. Central concepts of geometric
sampling and estimation will be introduced at the beginning. Sampling
protocols are also demonstrated on real tissue in several Laboratory sessions.

Instructors: L.M. Cruz-Orive, M. Geiser, M. Ochs.
Details and registration: http://www.ana.unibe.ch/event/ster/index.html





Matthias Ochs, M.D.
Institute of Anatomy
Experimental Morphology Unit
University of Bern
Baltzerstr. 2
CH-3000 Bern 9
Switzerland
Phone: +41 31 631 4624
Fax: +41 31 631 3807
E-mail: ochs-at-ana.unibe.ch

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From: mccaulak-at-wfu.edu
Date: Wed, 27 Jul 2005 12:11:28 -0500
Subject: [Microscopy] Re: user charges for SEM and related

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Well, now that hopefully everyone is finished beating me down and
questioning my ethics, I'd like to offer a bit more of an explanation into
the reason for my posted question.

First of all, my facility exists to serve my departments needs and those
needs alone. The "outsider" in question is a former student who works at
another non-profit and who is establishing a pedagogical relationship with
us which will include teaching, mentoring, and graduate student research.
This person simply asked about providing monetary compensation if the need
arose. I was at a loss for how to answer and so thought I would ask the
listserv about this.

This is not a situation in which private labs are being undercut or a
situation in which an academic lab is pursing commercial sources of funding.

Thanks to Debby for her instructive post on an appropriate methodology for
arriving at a cost structure.

AM



-----Original Message-----
X-from: tonygr-at-MIT.EDU [mailto:tonygr-at-MIT.EDU]
Sent: Wednesday, July 27, 2005 12:34 PM
To: mccaulak-at-wfu.edu

I have to say I was waiting for Chuck's response on this thread - my jaw
dropped when I read it, until the explanation came in the next e-mail!!!

More seriously--

Most of the discussion on this thread has been about the harm done to
commercial labs by university labs "undercutting", "poaching customers" -
or whatever description you want to apply, and by the need to determine the
"true" cost of providing the service. I think, though, that there is a
serious problem for the university labs themselves in allowing commercial
service activities to become an important part of their operation.

Any operation has a core mission. The proprietor of any successful
business knows the importance of identifying and maintaining their core
mission, and the ex-proprietors of many failed businesses know the costs of
straying from the mission. While individual organizations vary, the core
mission of a university characterization laboratory is not the same as that
of a commercial laboratory. In a university our basic mission is education
through the generation of research results; that of the company is to
generate a profit through providing an analytical service. These
differences, though in some areas subtle, lead to totally different
approaches to the way we interact with our clients, the way we manage our
instrumentation, our time, etc, etc.

The government (through the NSF, in my case) contributes towards the cost
of setting up and maintaining my characterization facilities. If we
generate first-class results on new and interesting materials, then this is
considered money well-spent. If I dilute this by taking on commercial
work, even if this leads to a reduction in the amount of "subsidy", I am
reducing the rationale for getting any support, and in general diluting the
reputation of my laboratories.

I realize that not everyone in an academic environment has my good fortune
to work for an administration that supports the ideas I have just
expressed, and I also stress again that we each work in unique
environments, but in general I would suggest that in most cases commercial
clients are best left to commercial laboratories. A commercial client is
generally looking for an analytical service, rather than to be educated.

Tony Garratt-Reed.


At 10:46 PM 7/26/2005, you wrote:



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From: jmkrupp-at-cats.ucsc.edu
Date: Wed, 27 Jul 2005 12:23:07 -0500
Subject: [Microscopy] stain solutions

Contents Retrieved from Microscopy Listserver Archives
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Hi:

I have a small lab, we do mostly basic EM type work.

Now a days, we don't do much sectioning for the TEM. Once and a while
someone wants me to do some sectioning, but many weeks (months) may pass
between requests.

I am trying to figure out a way to manage the post staining solutions so I
don't have to mix them up every time and/or how to make up just a little
for the occasional job.

Any neat tricks or systems for storing post stains like lead citrate and
uranyl acetate out there?

How about storage life time and/or ideas about mixing minimum quantities.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: gwe-at-ufl.edu
Date: Wed, 27 Jul 2005 12:25:46 -0500
Subject: [Microscopy] user charges for SEM and related

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One issue on this topic is that those of us at state institutions
have an obligation to serve the taxpayers of our state as well as the
scientists on our respective campuses. Agricultural extension services
are an example of organized fulfillment of such obligations. Many of
their services are absolutely free. When external customers come to us
they are more often looking for help with the science behind their
projects rather than merely an analytical service. When their tax
dollars support our salaries and other costs, they can certainly expect
a break. These relationships could be interpreted as
contracts-for-research, which are perfectly legitimate arrangements.

Greg Erdos

tonygr-at-MIT.EDU wrote:

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From: redhair-at-stanford.edu
Date: Wed, 27 Jul 2005 12:47:43 -0500
Subject: [Microscopy] Re: stain solutions

Contents Retrieved from Microscopy Listserver Archives
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Hi Jon. One of the best tricks I have found over the years is to weigh out
small amounts of lead citrate (0.1 to 0.4 grams) into 15 ml centrifuge
tubes. When you need to make stain, add 1ml of carbonate free 1N NaOH to
the tube to dissolve the lead(solution should be clear). Then add 9 mls of
dd water and shake well. Put solution through a syringe filter and it is
ready for use. I stain 30 seconds to 1 minute. Never have a problem with
precipitate and stain is fresh every time. JoAnn
At 12:27 PM 7/27/2005 -0500, you wrote:



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650-723-5856


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From: gcc-at-couger.com
Date: Wed, 27 Jul 2005 12:49:04 -0500
Subject: [Microscopy] Re: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
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Your situation is more like extension at a land grant school
than work for hire.

And there are many ways to view it. If it is an ongoing thing
estimated the cost of materials and labor he will use what you
think it right then decide how high up the ladder you go to get
a decision.

If you use any services in a similar way use them for a pattern.

For things that cost small amounts of money time it often cost
less to give them away than to go the trouble of book keeping
and collecting them if no method is in place to do it.

I have see it done were the party need help bought supplies for
the department that helped him.

None of these are in line with accounting guide lines but use
good sense as their basis. I don't know how much trouble that
can get you in these days.

Gordon
Gordon Couger
Stillwater, OK
www.couger.com/gcouger

mccaulak-at-wfu.edu wrote:
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} Well, now that hopefully everyone is finished beating me down and
} questioning my ethics, I'd like to offer a bit more of an explanation into
} the reason for my posted question.
}
} First of all, my facility exists to serve my departments needs and those
} needs alone. The "outsider" in question is a former student who works at
} another non-profit and who is establishing a pedagogical relationship with
} us which will include teaching, mentoring, and graduate student research.
} This person simply asked about providing monetary compensation if the need
} arose. I was at a loss for how to answer and so thought I would ask the
} listserv about this.
}
} This is not a situation in which private labs are being undercut or a
} situation in which an academic lab is pursing commercial sources of funding.
}
} Thanks to Debby for her instructive post on an appropriate methodology for
} arriving at a cost structure.
}
} AM
}
}
}
} -----Original Message-----
} X-from: tonygr-at-MIT.EDU [mailto:tonygr-at-MIT.EDU]
} Sent: Wednesday, July 27, 2005 12:34 PM
} To: mccaulak-at-wfu.edu
} Subject: [Microscopy] Re: user charges for SEM and related
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I have to say I was waiting for Chuck's response on this thread - my jaw
} dropped when I read it, until the explanation came in the next e-mail!!!
}
} More seriously--
}
} Most of the discussion on this thread has been about the harm done to
} commercial labs by university labs "undercutting", "poaching customers" -
} or whatever description you want to apply, and by the need to determine the
} "true" cost of providing the service. I think, though, that there is a
} serious problem for the university labs themselves in allowing commercial
} service activities to become an important part of their operation.
}
} Any operation has a core mission. The proprietor of any successful
} business knows the importance of identifying and maintaining their core
} mission, and the ex-proprietors of many failed businesses know the costs of
} straying from the mission. While individual organizations vary, the core
} mission of a university characterization laboratory is not the same as that
} of a commercial laboratory. In a university our basic mission is education
} through the generation of research results; that of the company is to
} generate a profit through providing an analytical service. These
} differences, though in some areas subtle, lead to totally different
} approaches to the way we interact with our clients, the way we manage our
} instrumentation, our time, etc, etc.
}
} The government (through the NSF, in my case) contributes towards the cost
} of setting up and maintaining my characterization facilities. If we
} generate first-class results on new and interesting materials, then this is
} considered money well-spent. If I dilute this by taking on commercial
} work, even if this leads to a reduction in the amount of "subsidy", I am
} reducing the rationale for getting any support, and in general diluting the
} reputation of my laboratories.
}
} I realize that not everyone in an academic environment has my good fortune
} to work for an administration that supports the ideas I have just
} expressed, and I also stress again that we each work in unique
} environments, but in general I would suggest that in most cases commercial
} clients are best left to commercial laboratories. A commercial client is
} generally looking for an analytical service, rather than to be educated.
}
} Tony Garratt-Reed.
}
}
} At 10:46 PM 7/26/2005, you wrote:
}
}
}
}
} } ---------------------------------------------------------------------------
}
} -
}
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: lcgould-at-med.cornell.edu
Date: Wed, 27 Jul 2005 13:17:08 -0500
Subject: [Microscopy] Re: stain solutions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jon,
I make up my lead citrate using the Venable & Coggeshall method
(0.01% Pb citrate in 10 ml of water + 1 drop 10N NaOH) and store it
in a syringe that is wrapped in foil. If I keep the syringe wrapped
and its tip capped, the stain is quite stable for a long time. I use
a 0.2 micron filter on the syringe to dispense the stain.
I usually do en bloc staining with Ur Ac in water just before I begin
my dehydration steps, and so I can usually avoid staining the
sections with more Ur Ac. That solution too is pretty stable if kept
in a foil-wrapped bottle. I've been using a 1.5% solution, but there
was a recent thread on that topic where the concensus was that newer
bottles of "deplete" Ur Ac necessitated higher concentrations (up to
8%, if I remember correctly).
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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From: tina-at-pbrc.hawaii.edu
Date: Wed, 27 Jul 2005 13:24:55 -0500
Subject: [Microscopy] Re: stain solutions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Jonathan-

I make up 10 ml of each of my stains and store them in syringes fitted
with 0.2 micron filters, excluding air. They keep pretty well this way -
weeks to months at a time. I wrap the uranyl acetate syringe with aluminum
foil. I inspect for obvious precipitates before use. Expel several drops
through the filter before use.

Are you coming to M&M in Honolulu?

Aloha,
Tina


} I have a small lab, we do mostly basic EM type work.
}
} Now a days, we don't do much sectioning for the TEM. Once and a while
} someone wants me to do some sectioning, but many weeks (months) may pass
} between requests.
}
} I am trying to figure out a way to manage the post staining solutions so I
} don't have to mix them up every time and/or how to make up just a little
} for the occasional job.
}
} Any neat tricks or systems for storing post stains like lead citrate and
} uranyl acetate out there?
}
} How about storage life time and/or ideas about mixing minimum quantities.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


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From: jquinn-at-www.matscieng.sunysb.edu
Date: Wed, 27 Jul 2005 13:25:14 -0500
Subject: [Microscopy] re: user charges for SEM and related

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks

I have to slightly disagree with the long time
debate about academic vs commercial labs.

My scope was partially purchased with non-NSF funds.
Those funds were to encourage non-academic use.

Additionally, we have an administration that strongly
encourages us to help local industry with University
resources.

Infact, we have commercial consultants who use our equipment.

Hence, that is not competition.

regards,

JQuinn



**********************************************************
Dr. Jim Quinn james.quinn-at-stonybrook.edu
Materials Science 631-632-6663 FAX:8052
Stony Brook University www.matscieng.stonybrook.edu
Stony Brook, New York 11794 - 2275
**********************************************************


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From: bozzola-at-siu.edu
Date: Wed, 27 Jul 2005 13:25:28 -0500
Subject: [Microscopy] Re: Apology to the list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chuck,

I'm sure everyone laughed out loud at this posting.

Thanks for brightening up our day!!!

John


}
} Please accept my apologies for inadvertently sending out a message that was
} going to an individual and was obviously not intended for the list at large.
}
} I am embarrassed about this and will try to make sure this never happens
} again.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.2spi.com
} ########################
} ============================================
}
}
}
}
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--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################

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From: Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Wed, 27 Jul 2005 13:28:42 -0500
Subject: [Microscopy] cameras in labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

We are currently considering adding security cameras in each of our EM
labs.

We have a very large number of users, both during working hours and
after-hours in SEM and TEM rooms. Just recently, we have had a few
incidents where people have damaged the instrument and did not come
forward to admit it.

Although we can see who logged on to the computers, you can still damage
the instrument without logging in. We believe that a CCTV in each of the
labs would be the best way to solve this problem, but unfortunately we
are faced with "privacy issues". People do not want to be watched.

Please tell me if your labs use cameras (live or log) or if you found
other ways to get around the problem.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632


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From: walck-at-southbaytech.com
Date: Wed, 27 Jul 2005 13:49:54 -0500
Subject: [Microscopy] picoammeter availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is there anyone out in microscopy land that might be willing to let me
borrow a Keithley picoammeter. I wold like to do some experiments with
an ion gun. The unit that came with the Gatan analytical stage is what
I would like to get my hands on for a few days. I believe that the
model number is a 480. I am not adverse to going to my new boss with
some spirited horse trading if you can help me out, especially if you
might be willing to part with it permanently. If someone has an even
older model that they might want to part with like a 601 or 610, that
would be useful to me also.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


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From: zaluzec-at-microscopy.com
Date: Wed, 27 Jul 2005 14:00:03 -0500
Subject: [Microscopy] Re: cameras in labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Daniel

Have a look at the TelePresence Microscopy Site

http://tpm.amc.anl.gov

Use either Netscape or IE browsers , Safari access is currently broken I'll fix that
after the MM2005 meeting.

Nestor
Your Friendly Neighborhood SysOp



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
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8, 14 -- Date: Wed, 27 Jul 2005 14:00:01 -0500
8, 14 -- To: microscopy-at-microscopy.com
8, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
8, 14 -- Subject: Re: [Microscopy] cameras in labs
8, 14 -- Cc: Daniel.Salamon-at-nrc-cnrc.gc.ca
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==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Wed, 27 Jul 2005 15:06:30 -0500
Subject: [Microscopy] Re: rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear bmollon


How on earth do you read Nestor's email as "derogatory and demeaning"?

Do you see some equivalence between saying "I don't care who you are" and
describing someone else as practising "crap journalism"?

What color is the sky in your world?

And how about having the courage to identify yourself?

Anonymous postings don't rate very highly with most people.

cheers

rtch




Date sent: Wed, 27 Jul 2005 09:42:24 -0500
To: r.sims-at-auckland.ac.nz
X-from: bmollon-at-pacbell.net
Send reply to: microscopy-at-microscopy.com

Hey guys,
I thing some of this is coming down to the issue of reading
tone/intent into words in print. When Nestor wrote "I don't care who
you are" , MY interpretation was "no one has special rights or
privileges" not "I don't give a damn about you". (Just my humble
reading).
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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1, 22 -- Date: Wed, 27 Jul 2005 16:06:23 -0400
1, 22 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
1, 22 -- Subject: [Microscopy] Re: rules
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From: K.venner-at-ion.ucl.ac.uk
Date: Wed, 27 Jul 2005 15:11:44 -0500
Subject: [Microscopy] viaWWW: tem flat embedding moulds

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Wednesday, July 27, 2005 at 10:27:20
---------------------------------------------------------------------------

Email: K.venner-at-ion.ucl.ac.uk
Name: Kerrie

Organization: ION, UCL, UK

Title-Subject: [Filtered] tem flat embedding moulds

Question: I have some well used, much coveted flat embedding moulds for TEM samples. They are shallow, hexagonal-shaped recesses with an arrowhead on one side, and are in a pale blue rubbery material. Unfortunately they are beginning to perish, and we have no idea where we bought them from originally. We are desperate to replace them. Any ideas or suppliers out there?

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Wed Jul 27 15:11:44 2005
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6, 12 -- From: K.venner-at-ion.ucl.ac.uk (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: tem flat embedding moulds
6, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: deerinck-at-ncmir.ucsd.edu
Date: Wed, 27 Jul 2005 15:13:26 -0500
Subject: [Microscopy] viaWWW: Staff Positions Available at the National Center for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (deerinck-at-ncmir.ucsd.edu) from http://microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, July 27, 2005 at 12:48:11
---------------------------------------------------------------------------

Email: deerinck-at-ncmir.ucsd.edu
Name: Tom Deerinck

Organization: NCMIR, UCSD

Title-Subject: [Filtered] MListserver:

Question: Staff Positions Available at the National Center for Microscopy and Imaging Research at the University of California, San Diego, CA

Two staff positions in TEM and 3D tomographic reconstruction are available immediately. The successful candidate should have a BS or MS in relevant fields of biological sciences and have experience in either 1) transmission electron microscopy or 2) image processing and/or three-dimensional reconstruction techniques.

Qualifications for the first position (Transmission Electron Microscopist) are:
_ Experienced TEM operation (operation, alignment, image recording)
_ Proficiency in sectioning of plastic embedded biological specimens (microtomy)
_ Familiarity in preparation of biological material for plastic embedding (fixation, dehydration and embedding techniques)
_ Experience in bacteriology, immunocytochemistry, cell biology or fluorescence/confocal microscopy preferred but not essential.

Qualifications for the second position (Image Processing Scientist) are:
_ Experience in image processing and evaluating EM images
_ Experience in three-dimensional tomographic reconstruction
_ Familiarity using Linux or Unix, Mac and PC operating systems
_ Proficiency in computer visualization and/or animation package
_ General biology background with a preferred emphasis on bacterial systems.

Excellent written and verbal skills are expected. The candidates will be working in a large interdisciplinary research center located in the University of California San Diego main campus and will have access to state of the art electron microscopes. For more information on our laboratory, please visit http://www.ncmir.ucsd.edu.
Please forward this message to all appropriate personnel. Applicants should send curriculum vitae, bibliography, a brief description of present research activities and plans and the names and contact information of three references to:

Dr. Mark Ellisman or Mr. Thomas Deerinck
University of California at San Diego
1000 Basic Science Building MC 0608
9500 Gilman Drive
La Jolla, CA 92093-0608
858-534-4583 (phone) mark-at-ncmir.ucsd.edu (email)
858-534-7497 (fax) deerinck-at-ncmir.ucsd.edu

We will also be available at the MSA meeting next week in Hawaii.


---------------------------------------------------------------------------

==============================Original Headers==============================
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13, 13 -- To: microscopy-at-microscopy.com
13, 13 -- From: deerinck-at-ncmir.ucsd.edu (by way of MicroscopyListserver)
13, 13 -- Subject: viaWWW: Staff Positions Available at the National Center for
13, 13 -- Microscopy and Imaging Research UCSD
13, 13 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: lcgould-at-med.cornell.edu
Date: Wed, 27 Jul 2005 15:25:38 -0500
Subject: [Microscopy] Re: viaWWW: tem flat embedding moulds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

They sound like Chien molds to me....I know that EMS carries them,
probably other suppliers too.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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1, 23 -- Date: Wed, 27 Jul 2005 16:25:26 -0400
1, 23 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu}
1, 23 -- Subject: Re: [Microscopy] viaWWW: tem flat embedding moulds
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From: leswes-at-shaw.ca
Date: Wed, 27 Jul 2005 15:57:31 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'll second this.

--
Lesley Weston


} From: Winston.Wiggins-at-cshs.org
} Reply-To: microscopy-at-microscopy.com
} Date: Wed, 27 Jul 2005 10:54:20 -0500
} To: leswes-at-shaw.ca
} Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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}
} List members et al.,
} Is there any way we can "restore" the system to the point before the I.Q.
} posting, thereby returning Sergey to the List? I would hate to miss his
} often insightful comments. I also hate to think that any comment, understood
} or misunderstood, made in the heat of passion may summarily cause expulsion
} sans appeal, deservedly or not.
} "Can we all just get along?!"
} Winston
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Winston W. Wiggins, E.M. Pathology Asst.
} CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
} Electron Microscopy, LLSPT, A823-A828
} 8700 Beverly Blvd.
} Los Angeles, CA 90048
} 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
} Winston.Wiggins-at-CSHS.org
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
} -----Original Message-----
} X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
} Sent: Tuesday, July 26, 2005 5:12 PM
} To: Winston.Wiggins-at-CSHS.org
} Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Person
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} ----------------------------------------------------------------------------
}
} Sergey
}
} A honest discussion or even disagreement about a subject or work which is
} microscopy
} related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally disparage any person
} or organization on the Listserver. This rule applies equally to
} Journalists as well
} as Microscopists. Please review the rules which you received upon
} subscription.
}
}
} http://microscopy.com/MicroscopyListserver/Rules.html
}
} Presumably a person of hi IQ has the ability to distinguish between these
} two.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
} } ---------------------------------------------------------------------------
} -
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} } ---------------------------------------------------------------------------
} -
} }
} } Nestor
} } I don't see any reason why we could not discuss the quality of somebody's
} } work even if it's a journalist with former (?) high IQ. Is it prohibited
} } to discuss people's work with high IQ on this ListServer? Sergey
} }
} } At 03:51 PM 7/26/2005, you wrote:
} } ---------------------------------------------------------------------------
} -
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} http://www.microscopy.com/MicroscopyListserver
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} } } --------------------------------------------------------------------------
} --
} } }
} } } Beth & everyone else
} } }
} } } There was no problem with Beth's initial posting and no apology
} } } was needed here Beth. Sorry if I appeared to have come down on you.
} } }
} } } The followups IMHO were starting to drift into critiques of Marilyn
} whomever
} } } and her "crap Journalism" and I was trying to nip that direction of
} } } critique in the bud. However, I see I had the opposite effect. Oh
} well.....
} } }
} } } As Dorrance said, everyone can use a grin occassionally .
} } }
} } }
} } } Nestor
} } }
} } }
} } } --------------------------------------------------------------------------
} --
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
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} } ---------------------------------------------------------------------------
} -
} } } }
} } } } I apologize to the list for posting Marilyn vos Savant's statement on
} } } } microscopy. I just wanted to share the article because I thought it was
} } } } humorous...I shall refrain from such postings.
} } } } best,
} } } } Beth
} } } }
} } } } **********************************************************************
} } } } Beth Richardson
} } } } EM Lab Coordinator
} } } } Plant Biology Department
} } } } University of Georgia
} } } } Athens, GA 30602-7271
} } } }
} } } } Phone - (706) 542-1790 & FAX - (706) 542-1805
} } } } http://www.plantbio.uga.edu/emlab
} } } }
} } } } "Between the two evils,
} } } } I always pick the one I never tried before". Mae West (1893-1980)
} } } } *******************************************************************
} } } }
} } } } "And it's only the giving that makes you what you are".
} } } } Wond'ring Aloud, Jethro Tull (Aqualung)
} } } }
} } } } ************************************************************************
}
}
}
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} ==============================Original Headers==============================
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} 18, 25 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org}
} 18, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
} 18, 25 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of
} a Pers
} 18, 25 -- on
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==============================Original Headers==============================
5, 27 -- From leswes-at-shaw.ca Wed Jul 27 15:57:28 2005
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5, 27 -- Date: Wed, 27 Jul 2005 13:58:42 -0700
5, 27 -- From: Lesley Weston {leswes-at-shaw.ca}
5, 27 -- Subject: Re: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers
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From: DFORAN-at-ORA.FDA.GOV
Date: Wed, 27 Jul 2005 16:00:11 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Aye.

-----Original Message-----
X-from: leswes-at-shaw.ca [mailto:leswes-at-shaw.ca]
Sent: Wednesday, July 27, 2005 3:58 PM
To: Foran, David A

I'll second this.

--
Lesley Weston


} From: Winston.Wiggins-at-cshs.org
} Reply-To: microscopy-at-microscopy.com
} Date: Wed, 27 Jul 2005 10:54:20 -0500
} To: leswes-at-shaw.ca
} Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a
} Pers
}
}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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}
} List members et al.,
} Is there any way we can "restore" the system to the point before the
} I.Q. posting, thereby returning Sergey to the List? I would hate to
} miss his often insightful comments. I also hate to think that any
} comment, understood or misunderstood, made in the heat of passion may
} summarily cause expulsion sans appeal, deservedly or not. "Can we all
} just get along?!" Winston
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Winston W. Wiggins, E.M. Pathology Asst.
} CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron
} Microscopy, LLSPT, A823-A828 8700 Beverly Blvd.
} Los Angeles, CA 90048
} 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
} Winston.Wiggins-at-CSHS.org
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
} -----Original Message-----
} X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
} Sent: Tuesday, July 26, 2005 5:12 PM
} To: Winston.Wiggins-at-CSHS.org
} Subject: [Microscopy] Discussion of high IQ vs Disparagement of a
} Person
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Sergey
}
} A honest discussion or even disagreement about a subject or work
} which is microscopy related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally disparage any person
} or organization on the Listserver. This rule applies equally to
} Journalists as well as Microscopists. Please review the rules which
} you received upon
} subscription.
}
}
} http://microscopy.com/MicroscopyListserver/Rules.html
}
} Presumably a person of hi IQ has the ability to distinguish between
} these two.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
} } ---------------------------------------------------------------------
} } ------
} -
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
---------------------------------------------------------------------------
} -
} }
} } Nestor
} } I don't see any reason why we could not discuss the quality of
} } somebody's work even if it's a journalist with former (?) high IQ.
} } Is it prohibited to discuss people's work with high IQ on this
} } ListServer? Sergey
} }
} } At 03:51 PM 7/26/2005, you wrote:
} } ---------------------------------------------------------------------
} } ------
} -
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } --------------------------------------------------------------------
} } } ------
} --
} } }
} } } Beth & everyone else
} } }
} } } There was no problem with Beth's initial posting and no apology
} } } was needed here Beth. Sorry if I appeared to have come down on you.
} } }
} } } The followups IMHO were starting to drift into critiques of
} } } Marilyn
} whomever
} } } and her "crap Journalism" and I was trying to nip that direction of
} } } critique in the bud. However, I see I had the opposite effect. Oh
} well.....
} } }
} } } As Dorrance said, everyone can use a grin occassionally .
} } }
} } }
} } } Nestor
} } }
} } }
} } } --------------------------------------------------------------------
} } } ------
} --
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} } ---------------------------------------------------------------------
} } ------
} -
} } } }
} } } } I apologize to the list for posting Marilyn vos Savant's statement
} } } } on microscopy. I just wanted to share the article because I thought
} } } } it was humorous...I shall refrain from such postings. best,
} } } } Beth
} } } }
} } } } *******************************************************************
} } } } ***
} } } } Beth Richardson
} } } } EM Lab Coordinator
} } } } Plant Biology Department
} } } } University of Georgia
} } } } Athens, GA 30602-7271
} } } }
} } } } Phone - (706) 542-1790 & FAX - (706) 542-1805
} } } } http://www.plantbio.uga.edu/emlab
} } } }
} } } } "Between the two evils,
} } } } I always pick the one I never tried before". Mae West (1893-1980)
} } } } *******************************************************************
} } } }
} } } } "And it's only the giving that makes you what you are". Wond'ring
} } } } Aloud, Jethro Tull (Aqualung)
} } } }
} } } } *******************************************************************
} } } } *****
}
}
}
}
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} 18, 25 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement
of
} a Pers
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==============================Original Headers==============================
5, 27 -- From leswes-at-shaw.ca Wed Jul 27 15:57:28 2005
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5, 27 -- Subject: Re: [Microscopy] RE: Discussion of high IQ vs
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==============================Original Headers==============================
12, 17 -- From DFORAN-at-ORA.FDA.GOV Wed Jul 27 16:00:11 2005
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12, 17 -- Subject: RE: [Microscopy] Discussion of high IQ vs Disparagement of a Per
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From: tiekotte-at-up.edu
Date: Wed, 27 Jul 2005 16:02:23 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

aye


On 7/27/05 2:00 PM, "DFORAN-at-ORA.FDA.GOV" {DFORAN-at-ORA.FDA.GOV} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Aye.
}
} -----Original Message-----
} X-from: leswes-at-shaw.ca [mailto:leswes-at-shaw.ca]
} Sent: Wednesday, July 27, 2005 3:58 PM
} To: Foran, David A
} Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers
}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I'll second this.


==============================Original Headers==============================
5, 18 -- From tiekotte-at-up.edu Wed Jul 27 16:02:23 2005
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5, 18 -- Date: Wed, 27 Jul 2005 14:02:21 -0700
5, 18 -- Subject: Re: [Microscopy] RE: Discussion of high IQ vs Disparagement of a
5, 18 -- Per
5, 18 -- From: Ken Tiekotter {tiekotte-at-up.edu}
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From: Tom.Januszewski-at-UTSouthwestern.edu
Date: Wed, 27 Jul 2005 16:14:16 -0500
Subject: [Microscopy] Re: viaWWW: tem flat embedding moulds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

They are Chien embedding molds. We got ours from Ted Pella.
Tom

Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
Dallas, TX 75390-9039
214-648-7291
FAX 214-648-6408
tom.januszewski-at-UTSouthwestern.edu


==============================Original Headers==============================
3, 17 -- From Tom.Januszewski-at-UTSouthwestern.edu Wed Jul 27 16:14:16 2005
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3, 17 -- From: "Tom Januszewski" {Tom.Januszewski-at-UTSouthwestern.edu}
3, 17 -- To: {microscopy-at-microscopy.com}
3, 17 -- Subject: Re: [Microscopy] viaWWW: tem flat embedding moulds
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From: DusevichV-at-umkc.edu
Date: Wed, 27 Jul 2005 16:25:16 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Per

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Aye

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} -----Original Message-----
} From: DFORAN-at-ORA.FDA.GOV [mailto:DFORAN-at-ORA.FDA.GOV]
} Sent: Wednesday, July 27, 2005 4:01 PM
} To: Dusevich, Vladimir
} Subject: [Microscopy] RE: Discussion of high IQ vs
} Disparagement of a Per
}
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Aye.
}
} -----Original Message-----
} X-from: leswes-at-shaw.ca [mailto:leswes-at-shaw.ca]
} Sent: Wednesday, July 27, 2005 3:58 PM
} To: Foran, David A
} Subject: [Microscopy] Discussion of high IQ vs Disparagement of a Pers
}
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} I'll second this.
}
} --
} Lesley Weston
}
}
} } From: Winston.Wiggins-at-cshs.org
} } Reply-To: microscopy-at-microscopy.com
} } Date: Wed, 27 Jul 2005 10:54:20 -0500
} } To: leswes-at-shaw.ca
} } Subject: [Microscopy] RE: Discussion of high IQ vs
} Disparagement of a
} } Pers
} }
} }
} }
} }
} }
} ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} --------------
} }
} } List members et al.,
} } Is there any way we can "restore" the system to the point before the
} } I.Q. posting, thereby returning Sergey to the List? I would hate to
} } miss his often insightful comments. I also hate to think that any
} } comment, understood or misunderstood, made in the heat of
} passion may
} } summarily cause expulsion sans appeal, deservedly or not.
} "Can we all
} } just get along?!" Winston
} }
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Winston W. Wiggins, E.M. Pathology Asst.
} } CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron
} } Microscopy, LLSPT, A823-A828 8700 Beverly Blvd.
} } Los Angeles, CA 90048
} } 310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
} } Winston.Wiggins-at-CSHS.org
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} }
} } -----Original Message-----
} } X-from: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com]
} } Sent: Tuesday, July 26, 2005 5:12 PM
} } To: Winston.Wiggins-at-CSHS.org
} } Subject: [Microscopy] Discussion of high IQ vs Disparagement of a
} } Person
} }
} }
} ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} --------------
} }
} } Sergey
} }
} } A honest discussion or even disagreement about a subject or work
} } which is microscopy related (even tangentially) is not prohibited.
} }
} } However, it is against the rules to intentionally disparage
} any person
} } or organization on the Listserver. This rule applies equally to
} } Journalists as well as Microscopists. Please review the
} rules which
} } you received upon
} } subscription.
} }
} }
} } http://microscopy.com/MicroscopyListserver/Rules.html
} }
} } Presumably a person of hi IQ has the ability to distinguish between
} } these two.
} }
} } Nestor
} } Your Friendly Neighborhood SysOp
} }
} } }
} ---------------------------------------------------------------------
} } } ------
} } -
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} --------------------------------------------------------------
} -------------
} } -
} } }
} } } Nestor
} } } I don't see any reason why we could not discuss the quality of
} } } somebody's work even if it's a journalist with former (?)
} high IQ.
} } } Is it prohibited to discuss people's work with high IQ on this
} } } ListServer? Sergey
} } }
} } } At 03:51 PM 7/26/2005, you wrote:
} } }
} ---------------------------------------------------------------------
} } } ------
} } -
} } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} --------------------------------------------------------------------
} } } } ------
} } --
} } } }
} } } } Beth & everyone else
} } } }
} } } } There was no problem with Beth's initial posting and no apology
} } } } was needed here Beth. Sorry if I appeared to have come
} down on you.
} } } }
} } } } The followups IMHO were starting to drift into critiques of
} } } } Marilyn
} } whomever
} } } } and her "crap Journalism" and I was trying to nip that
} direction of
} } } } critique in the bud. However, I see I had the opposite effect. Oh
} } well.....
} } } }
} } } } As Dorrance said, everyone can use a grin occassionally .
} } } }
} } } }
} } } } Nestor
} } } }
} } } }
} } } }
} --------------------------------------------------------------------
} } } } ------
} } --
} } } } } The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of
} } America
} } } } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } } } On-Line Help


==============================Original Headers==============================
7, 23 -- From DusevichV-at-umkc.edu Wed Jul 27 16:25:16 2005
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From: cgarber-at-2spi.com
Date: Wed, 27 Jul 2005 17:12:36 -0500
Subject: [Microscopy] University facilities being used for outside users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Tony Garratt-Reed wrote:
========================================================
I have to say I was waiting for Chuck's response on this thread - my jaw
dropped when I read it, until the explanation came in the next e-mail!!!
========================================================
Perhaps I became a bit "gun shy" after sending off a message in error. I
appreciate the understanding of those on the list for someone, like myself,
who did make an error. It has taken me more than a few hours to recover
from that.

I have operated an independent testing and analytical laboratory with its
core capabilities being built around SEM/EDS and TEM and LM since 1970. I
think I have at least some credentials to speak to the issue at hand.
However, what I have to say might be seen as "too much" for a normal
listserver posting, therefore I have posted my "reply" to URL
http://www.2spi.com/letter.html

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
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From: J.Nailon-at-uq.edu.au
Date: Wed, 27 Jul 2005 17:44:01 -0500
Subject: [Microscopy] Re: cameras in labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

G'day Daniel,

We used to showoff our instrumentation across the web through our
Nanoworld site, we have 14 EM columns and most were available to view
24/7. This was until we had a nasty stalking incident that left both the
client involved and our staff members unsettled until we took all
cameras off air. We now do individual presentation to schools and groups
as required and everyone is aware the cameras are ON.

Regards
JVN



Daniel.Salamon-at-nrc-cnrc.gc.ca wrote:

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From: gary-at-gaugler.com
Date: Wed, 27 Jul 2005 18:19:32 -0500
Subject: [Microscopy] Trolling for SEM/STEM specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am again looking for prepared and interesting
specimens for SEM/STEM or just TEM negs. Human pathogens
are especially welcome. Standard 12mm pin stubs for
SEM are perfect. Bacteria, parasites, cancers, etc.
are good. CPD/fixed specimens coated or un-coated
are fine.

Pls contact me off-line for my payment schedule
and what you may have to sell (I keep it) or rent
(in case you want it back).

See you in HI.

gary g.


==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Wed, 27 Jul 2005 19:30:42 -0500
Subject: [Microscopy] Re: golden rule applies here

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

isn't the golden rule do uuto others before they do
unto you?
which clearly seems to be the case in a preemptive
attack

--- frank.karl-at-degussa.com wrote:

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__________________________________________________
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Tired of spam? Yahoo! Mail has the best spam protection around
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From: elshaw-at-MIT.EDU
Date: Wed, 27 Jul 2005 22:35:21 -0500
Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For heaven's sake, stop your anonymous bitching!

This is a scientific forum, tirelessly, patiently, and good-humoredly run for the benefit
of us all by Nestor, who certainly doesn't deserve this, not some sort of internet chat
room.

Stop sniping or quit the list.

rtch




Date sent: Wed, 27 Jul 2005 19:32:16 -0500
To: r.sims-at-auckland.ac.nz
X-from: hoffpajo-at-yahoo.com
Send reply to: microscopy-at-microscopy.com

Note to Winston et al: I see nothing in the listserver FAQ that says
a revoked subscriber can't request to be resubscribed.

I have a suspicion that much of this brouhaha can be sourced to
record heat stewing the brains of hapless millions in the Lower 48
and southern Canada.

Hawaii sounds just perfect right about now. Y'all enjoy! The rest
of us will stand in front of the refrigerator with the door open ;^)

Libby Shaw


} Date: Wed, 27 Jul 2005 10:49:35 -0500
} From: Winston.Wiggins-at-cshs.org
} Subject: [Microscopy] RE: Discussion of high IQ vs Disparagement of a Pers
}
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From: neuberger1234-at-comcast.net
Date: Wed, 27 Jul 2005 22:55:21 -0500
Subject: [Microscopy] Discussion of high IQ thread

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Folks,

My delete key has certainly had a workout yesterday and today. I do hope that
tomorrow brings a respite from the "heat" generated by this thread and that we
can all move on with our lives. From personal experience, life can be way too
short to expend so much energy on this issue which in the end, I believe, will
have advanced the wisdom of humankind not one iota but seems to have created ill
feelings among some folks.

Please, if you wish to reply to this message, just reply to me and not the
entire list and give this thread and the list a well deserved rest!

Damian Neuberger




==============================Original Headers==============================
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From: tanderso-at-cbs.umn.edu
Date: Thu, 28 Jul 2005 00:49:10 -0500
Subject: [Microscopy] Re: University facilities being used for outside

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The beauty of this board is the ability to disseminate the
knowledge of microscopy. This has proven useful to me as well as countless
others across the globe.

That being said, I think some of us across this board need to lighten up.

If certain things said here trigger specific emotions personally, then it's
time to bring those things up to your personal psychologist, not this
listserv.

My trigger finger is getting tired of hitting the delete key to these
emotional responses... can't we just get back to microscopy, the purpose of
this board??!

- Tracy


On 7/27/05 5:16 PM, "cgarber-at-2spi.com" {cgarber-at-2spi.com} wrote:

}
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} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Tony Garratt-Reed wrote:
} ========================================================
} I have to say I was waiting for Chuck's response on this thread - my jaw
} dropped when I read it, until the explanation came in the next e-mail!!!
} ========================================================
} Perhaps I became a bit "gun shy" after sending off a message in error. I
} appreciate the understanding of those on the list for someone, like myself,
} who did make an error. It has taken me more than a few hours to recover
} from that.
}
} I have operated an independent testing and analytical laboratory with its
} core capabilities being built around SEM/EDS and TEM and LM since 1970. I
} think I have at least some credentials to speak to the issue at hand.
} However, what I have to say might be seen as "too much" for a normal
} listserver posting, therefore I have posted my "reply" to URL
} http://www.2spi.com/letter.html
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.2spi.com
} ########################
} ============================================
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}
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}
} ==============================Original Headers==============================
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} 7, 13 -- Subject: University facilities being used for outside users
} 7, 13 -- Date: Wed, 27 Jul 2005 18:12:49 -0500
} 7, 13 -- From: "Garber, Charles A." {cgarber-at-2spi.com}
} 7, 13 -- X-Mailer: E-Mail Connection v3.1a
} ==============================End of - Headers==============================

-----
Tracy E. Anderson
Microscopist / Imaging Specialist
Imaging Center
University of Minnesota
Phone: 612.624.3454
Fax: 612.624.1799
http://www.cbs.umn.edu/ic/

³Science and art belong to the whole world, and before them vanish the
barriers of nationality.² - Goethe





==============================Original Headers==============================
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From: tanderso-at-cbs.umn.edu
Date: Thu, 28 Jul 2005 01:00:51 -0500
Subject: [Microscopy] Zoinks

Contents Retrieved from Microscopy Listserver Archives
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I realize now that my previous message doesn't directly relate to the
subject line. It was mainly in response to the "IQ" post, which I thought
was interesting and humorous to read.

Bon soiree







==============================Original Headers==============================
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From: Gretchen.Ziegler-at-leica-microsystems.com
Date: Thu, 28 Jul 2005 01:01:16 -0500
Subject: [Microscopy] Gretchen Ziegler/USDER/West/Leica is out of the office.

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office starting 07/28/2005 and will not return until
08/10/2005.

I am on vacation and not able to pick up emails or voicemails. If you need
immediate help please contact customer service in Bannockburn, otherwise I
will respond on August 10th.
Thank you.
Gretchen


_____________________________________________________________________
This e-mail has been scanned for viruses by MCI's Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.mci.com

==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 07:24:11 -0500
Subject: [Microscopy] Re: golden rule applies here

Contents Retrieved from Microscopy Listserver Archives
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You know I just knew you couldn't resist replying the
email.
First of all there is nothing anonymous about my
email. I do believe my name appears in fron of the
email address.
2nd I think you refered to yahoo as a freebie email
account, while yes it is true I do not pay a cent for
the account, I do however pay a very high price for my
cable broadband account. How many of you on this list
server can say that when they post a message, that has
very little to do with work?
3rd you have no clue who you are talking to. I have 25
years in electron microscopy. I did however retire
from the field 2 years ago at the ripe old age of 46,
mainly to get away from people like you. I do what I
want when I want and with whomever I want. Now if you
are trying to run off those of us with experience and
something to contribute, with those you disagree you
are doing a good job.
You sir seem to know a lot more about internet chat
rooms than I do.

Finally: I leave it to Nestor to decide if your
account should be pulled for using DISPARAGING
REMARKS directed at me, quoteing you "anonymous
bitching!" I suspect he will do nothing.
Now having said all that, if you having anything
further to say to me on the subject email me directly.
I will be happy to explain myself with full vigor.
John Hoffpauir ret
637 pine street (society hill)
philadelphia pa
19106
PS does this make you happy?


--- r.sims-at-auckland.ac.nz wrote:

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} For heaven's sake, stop your anonymous bitching!
}
} This is a scientific forum, tirelessly, patiently,
} and good-humoredly run for the benefit
} of us all by Nestor, who certainly doesn't deserve
} this, not some sort of internet chat
} room.
}
} Stop sniping or quit the list.
}
} rtch
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}
}
} Date sent: Wed, 27 Jul 2005 19:32:16 -0500
} To: r.sims-at-auckland.ac.nz
} X-from: hoffpajo-at-yahoo.com
} Send reply to: microscopy-at-microscopy.com
} Subject: [Microscopy] Re: golden rule
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} } isn't the golden rule do uuto others before they
} do
} } unto you?
} } which clearly seems to be the case in a preemptive
} } attack
} }
} } --- frank.karl-at-degussa.com wrote:
} }
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} } }
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} } you. } } } ==============================Original
} }
} } Headers============================== } 12, 17 --
} From
} } frank.karl-at-degussa.com Wed Jul 27 } 11:46:02 2005
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} } ==============================Original
} } Headers============================== 5, 19 --
} From hoffpajo-at-yahoo.com
} } Wed Jul 27 19:30:42 2005 5, 19 -- Received: from
} } web50201.mail.yahoo.com (web50201.mail.yahoo.com
} [206.190.38.42]) 5,
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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 07:27:27 -0500
Subject: [Microscopy] Re: Discussion of high IQ thread

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


you are correct, i did however find the need to write
to this sims guy i note you have one of those
anonymous email accout he rails against.
john
--- neuberger1234-at-comcast.net wrote:

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} Folks,
}
} My delete key has certainly had a workout yesterday
} and today. I do hope that
} tomorrow brings a respite from the "heat" generated
} by this thread and that we
} can all move on with our lives. From personal
} experience, life can be way too
} short to expend so much energy on this issue which
} in the end, I believe, will
} have advanced the wisdom of humankind not one iota
} but seems to have created ill
} feelings among some folks.
}
} Please, if you wish to reply to this message, just
} reply to me and not the
} entire list and give this thread and the list a well
} deserved rest!
}
} Damian Neuberger
}
}
}
}
} ==============================Original
} Headers==============================
} 7, 16 -- From neuberger1234-at-comcast.net Wed Jul 27
} 22:55:20 2005
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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 07:34:04 -0500
Subject: [Microscopy] Re: my $0.02 worth on disparagement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

there is nothing to criticize, Sergey was censored.
john from an anaoymous email account

--- Jane.LaGoy-at-bodycote.com wrote:

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} I was enjoying the IQ discourse when it was
} light-hearted, but I don't
} believe the Microscopy Listserver, or any other
} professional listserver,
} should disparage people personally. It is not a
} question of censorship but
} of human decency. What is it our parents said? "If
} you can't say something
} nice about someone then don't say anything at all".
} The IQ lady publicly
} wrote her opinion, so that leaves those words as
} open game for criticism. I
} think it is unfortunate that Sergey was not allowed
} to make his own choice
} about leaving the list; in that way, he was
} censored. This is my personal
} opinion (and since I submitted it, you folks are all
} welcome to criticize
} it.)
}
} Jane L. LaGoy
} R&D Engineer
} Bodycote HIP
} 155 River Street
} Andover, MA 01810
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} ==============================Original
} Headers==============================
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} 3, 15 -- To: "Microscopy Listserver (E-mail)"
} {Microscopy-at-MSA.Microscopy.com}
} 3, 15 -- Subject: my $0.02 worth on disparagement
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] my $0.02 worth on disparagement
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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 07:39:34 -0500
Subject: [Microscopy] my $0.02 worth on disparagement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

opps i was trying to send that one directly to the
poster, heaven forbit i start a new thread, my
apology.

--- hoffpajo-at-yahoo.com wrote:

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} there is nothing to criticize, Sergey was censored.
} john from an anaoymous email account
}
} --- Jane.LaGoy-at-bodycote.com wrote:
}
} }
} }
} }
} }
}
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} }
} } I was enjoying the IQ discourse when it was
} } light-hearted, but I don't
} } believe the Microscopy Listserver, or any other
} } professional listserver,
} } should disparage people personally. It is not a
} } question of censorship but
} } of human decency. What is it our parents said?
} "If
} } you can't say something
} } nice about someone then don't say anything at
} all".
} } The IQ lady publicly
} } wrote her opinion, so that leaves those words as
} } open game for criticism. I
} } think it is unfortunate that Sergey was not
} allowed
} } to make his own choice
} } about leaving the list; in that way, he was
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} } Jane L. LaGoy
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From: hinmeigeng-at-hotmail.com
Date: Thu, 28 Jul 2005 09:13:10 -0500
Subject: [Microscopy] RE: saturated NaOH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

* * * * * * * * *
"I'm trying to express in grams the amount of NaOH that I'd need for 100 ml
of saturated NaOH in ethanol."
* * * * * * * * *
(1) I don't know the exact answer off-hand, but my first guess would be 20
grams in 100 ml ethanol.

(2) However, I do have experience of dissolving alkalies in various alcohols
to make etchants for micrscopy, and there are some things to watch out for:

(a) there will probably be a sodium carbonate crust which will not dissolve.

(b) the behaviour will be very dependent on the amount of water in the
ethanol. In fact, there is a possibility of phase separation into a strong
aqueous NaOH solution and the production of sodium ethoxide in the ethanolic
layer. Regarding ethanol, I read it on the web, but I do have experience
with the same reaction happening in butanol.

*** Of general interest ***

Potassium hydroxide (10% wt/vol) in isopropanol (propan-2-ol) is a jolly
good cleaner for getting charred organic residues off metals and glass. But
don't let it anywhere near aluminium, or you'll start an involuntary
preparation of aluminium isopropoxide.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



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From: TindallR-at-missouri.edu
Date: Thu, 28 Jul 2005 10:36:07 -0500
Subject: [Microscopy] TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Does anyone have experience with acetonitrile as a substitute transition
solvent for PO, or perhaps as the sole dehydration solvent in TEM prep?
I am checking the literature and archives, but any personal experiences
would be very useful.

Thanks!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







==============================Original Headers==============================
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From: baskin-at-bio.umass.edu
Date: Thu, 28 Jul 2005 10:41:53 -0500
Subject: [Microscopy] Re: TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
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Randy,
I tried dehydrating plant tissue for LM with acetonitrile
instead of ethanol and the results were terrible. The tissue was
preserved poorly. It was the root of arabidopsis.
Tobias

} ----------------------------------------------------------------------------
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From: AllanWojtasP-at-AGR.GC.CA
Date: Thu, 28 Jul 2005 11:00:02 -0500
Subject: [Microscopy] TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Randy,

I'd be interested, too, if you would share responses which aren't posted to the list.

Thanks in advance.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure/Chercheure scientifique, microstructure des aliments
Food Safety and Quality Team/Salubrité et qualité des aliments
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
32 Main Street/ 32 rue Main
Kentville, Nova Scotia/Kentville, (Nouvelle-Écosse)
Canada B4N 1J5
Telephone/Téléphone: (902) 679-5566
Facsimile/Télécopieur: (902) 679-2311

allanwojtasp-at-agr.gc.ca


Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Thursday, July 28, 2005 12:37 PM
To: Allan-Wojtas, Paula

Hi all,

Does anyone have experience with acetonitrile as a substitute transition
solvent for PO, or perhaps as the sole dehydration solvent in TEM prep?
I am checking the literature and archives, but any personal experiences
would be very useful.

Thanks!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







==============================Original Headers==============================
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From: AllanWojtasP-at-AGR.GC.CA
Date: Thu, 28 Jul 2005 11:03:55 -0500
Subject: [Microscopy] sorry for the post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, all,
 
I did it too, sent something to the wrong place......I'm sorry. Just getting ready to go on holidays and I acted in haste......
 
P.
 
Paula Allan-Wojtas
Research Scientist - Food Microstructure/Chercheure scientifique, microstructure des aliments
Food Safety and Quality Team/Salubrité et qualité des aliments
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
32 Main Street/ 32 rue Main
Kentville, Nova Scotia/Kentville, (Nouvelle-Écosse)
Canada B4N 1J5
Telephone/Téléphone: (902) 679-5566
Facsimile/Télécopieur: (902) 679-2311
 
allanwojtasp-at-agr.gc.ca
 
 

 


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From: lamiller-at-uiuc.edu
Date: Thu, 28 Jul 2005 12:34:26 -0500
Subject: [Microscopy] Re: TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
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I've found acetonitrile as a PO substitution, to work very well. Also,
it dissolves some plastics less readily, and is better for cell
cultures.

The results seem just as good if not better than PO in my situations. I
don't even use PO anymore.





On Jul 28, 2005, at 10:37 AM, TindallR-at-missouri.edu wrote:
} ----------------------------------------------------------------
}
} Hi all,
}
} Does anyone have experience with acetonitrile as a substitute
} transition
} solvent for PO, or perhaps as the sole dehydration solvent in TEM prep?
} I am checking the literature and archives, but any personal experiences
} would be very useful.
}
} Thanks!
}
} Randy


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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 12:35:34 -0500
Subject: [Microscopy] TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 12:36:14 -0500
Subject: [Microscopy] Re: TEM: dehydration solvents

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: gvrdolja-at-nature.berkeley.edu
Date: Thu, 28 Jul 2005 14:09:45 -0500
Subject: [Microscopy] 200kv TEM and field emission

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I've been normally using a 120kV TEM with a LaB6 filament to image
biological materials. I was using a 200kV tem with a field emission gun
recently and had problems with damage to the samples embedded in epon
araldite and formvar-C TEM grids. Is it necessary to use a different
embedding media with higher emission microscopes?
Gordon

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 14:10:53 -0500
Subject: [Microscopy] Re: 200kv TEM and field emission

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: tina-at-pbrc.hawaii.edu
Date: Thu, 28 Jul 2005 14:38:29 -0500
Subject: [Microscopy] Bus change for M&M Golf outing

Contents Retrieved from Microscopy Listserver Archives
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Hi, Listers-

I apologize for posting to all, but this is the only way we can get this
message to the participants in the M&M 2005 golf outing this weekend. The
computer of the organizer, Mark Sanders, is taking a vacation!

The bus situation has changed. Buses will *not* pick up participants at
the Hawaii Convention Center, but will now pickup at the Sheraton Waikiki
at 9:45 am and at the Hyatt Regency at 10:15, expected to get to Koolau
Golf Course at about 11:15 for a noon tee time.

There are still a few openings for this outing on Saturday and on Sunday;
if you are interested in joing them contact Mark Sanders via cell phone
612-867-5885 or by email when his computer is fixed, msanders-at-cbs.umn.edu

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************


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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 14:39:14 -0500
Subject: [Microscopy] Re: Bus change for M&M Golf outing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: dmclea-at-sandia.gov
Date: Thu, 28 Jul 2005 14:48:55 -0500
Subject: [Microscopy] M&M in Hawaii

Contents Retrieved from Microscopy Listserver Archives
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With you all flitting off to Hawaii could you PLEASE do me a favor and
not rub my nose in the fact that ya'll get to go on vacation and I have
to stay home. Please don't send the "LISTSERVER" an automatic "out of
office reply". I've worn the writing off my delete key this week.

Thanks very much,

Dorrance McLean
Intrepid GIRL scientist





Dorrance McLean
Microsystems Processing
Sandia National Laboratories
P.O. Box 969, MS 9401
Livermore, CA 94551-0969
925-294-3551 FAX 3870
Email dmclea-at-sandia.gov



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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 14:50:42 -0500
Subject: [Microscopy] Re: University facilities being used for outside users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hi Pat,

You have described what, IMHO, and probably that of most others, is a model
policy for appropriate use of university facilities.

Actually there are, within 100 miles of Philadelphia, probably half a dozen
for-profit independent laboratories focused (no pun intended) on microscopy
capabilities. Some of them are listed on our URL
http://www.2spi.com/catalog/hot-service7.html One of our main competitors
for the services is EMSL, right across the river from you in Camden.

There is nothing to my knowledge that says a nonprofit can not offer
services per se and receive a payment. But you have to become knowledgeable
about the tax code itself, and understand how the different classes of tax-
exempt institutions differ. Universities are in a separate category and are
restricted to doing those things that enhance educational objectives. When
the Congress some years ago recognized that some tax-exempt organizations
might engage in business/commercial activities, they enacted the UBIT
(unrelated business income tax). It was supposed to be the equalizer that
kept competition fair between such organizations (mainly not-for-profits or
so-called Section 501-(c)-3 organizations). It never worked very well (for
what for some could be interesting reasons), but the point was, UBIT was
never applied to those operating under the university exemption because
Congress just did not envision that a university ever would be engaging in a
business/commercial activity. In other words, even if a university
**wanted** to pay tax on some activity, there is absolutely no provision in
the current tax code for such a tax to be collected and paid!

The Franklin Institute case, to which you referred, was a special case. To
explain what happened would require a posting so long it would probably
cause Nestor's filters to see it as SPAM..... But they were a not-for-
profit (as opposed to universities in general being non-profits)
organization and they literally tried to drive Structure Probe, Inc. out of
the marketplace by offering rates for SEM services in the early 1970's so
low that no for-profit firm could survive. We were the plaintiff who filed
an anti-trust suit against them in 1972.

What did them in, and the main reason why their whole research laboratory is
no more is that their management people, at the trial and when under oath,
were saying things considerably different from when they were talking
informally to the IRS and others, like the newspapers. And the net result
was that the Franklin Institute Research Laboratories had to become Franklin
Institute Research Laboratories, Inc. And this meant they lost most of the
benefits they enjoyed as a not-for-profit tax-exempt organization. Putting
it another way, they had to pay the same taxes as any other business, they
had to double or triple their charge rates and once they started doing that,
they started losing customers, and their financial losses became a
hemorrhage (literally) and within a few years, they closed up shop and
disappeared.

I have given somewhat of a synopsis as to what happened but presumably the
court documents and filings are still part of the public record somewhere
and if one was interested, such information should be available should one
be interested in learning more.

What made this situation unique is that most institutional administrations
when confronted with an errant department engaging in inappropriate
activities on such a wholesale scale, would step in and say "whoaaaa" and
put a stop to it. But in this case, the officers and managers thought they
could literally run a tiny three person company out of money and therefore
out of business. They obviously failed, but because of that decision,
something like 600+ persons eventually lost their jobs when the FIRL, Inc.
closed down. The irony to me was that the officers and managers in the end
lost nothing. Those from prestigious Philadelphia industrial firms who sat
on their Board of Managers lost nothing. None of them were ever held
accountable. They were never punished. They just rode off into the sunset
with golden glove handshakes......

So that, in a nut shell, is the Philadelphia Story...... and the demise of
the Franklin Institute Research Laboratories.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================






----- Original Message -----
X-from: Pat Connelly
To: microscopy-at-microscopy.com
Cc: cgarber-at-2spi.com
Sent: Thursday, July 28, 2005 1:34 PM

I will be out of the office from July 28th returning to the office on August 8th, 2005.

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From: tivol-at-caltech.edu
Date: Thu, 28 Jul 2005 14:54:22 -0500
Subject: [Microscopy] Re: 200kv TEM and field emission

Contents Retrieved from Microscopy Listserver Archives
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On Jul 28, 2005, at 12:09 PM, gvrdolja-at-nature.berkeley.edu wrote:

} I've been normally using a 120kV TEM with a LaB6 filament to image
} biological materials. I was using a 200kV tem with a field emission
} gun
} recently and had problems with damage to the samples embedded in epon
} araldite and formvar-C TEM grids. Is it necessary to use a different
} embedding media with higher emission microscopes?
}
Dear Gordon,
Unless you were operating the 200 kV FEG at a much higher intensity
than the 120 kV LaB6, you shouldn't experience damage problems. One
can always use a lower-intensity beam (larger spot size number) and
spread the beam over a wide area so that a FEG will deliver a low dose
rate to the specimen. If you have an intense beam and a
correspondingly short exposure for your image, the beam can do damage
during the time that it is on the specimen but the image is not being
exposed. To clarify that last sentence, if you're scanning the grid
with the LaB6 beam, imaging with a 1 sec exposure, and you experience
no significant damage during the (say) 10-20 sec during which you're
focussing, framing the image, etc., then you go to a FEG beam with a
0.1 sec exposure, taking the same length of time to focus, etc., you
will have exposed your specimen to 10 times the dose, so there may well
be unacceptable damage. Otherwise, the 200 kV beam does less damage
per unit dose than the 120 kV beam, and there is nothing inherent in
the FEG that will increase the damage. Standard techniques for
producing plastic-embedded specimens should work as well for the 200 kV
FEG as for the 120 kV LaB6.
Yours,
Bill



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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 14:55:30 -0500
Subject: [Microscopy] 200kv TEM and field emission

Contents Retrieved from Microscopy Listserver Archives
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I will be out of the office from July 28th returning to the office on August 8th, 2005.

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1, 16 -- Received: from jeol.com (mail.jeol.com [208.196.228.78])
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1, 16 -- (envelope-from MAILER-DAEMON-at-jeol.com)
1, 16 -- From: "Patricia Corkum" {pcorkum-at-jeol.com}
1, 16 -- Date: Thu, 28 Jul 2005 15:55:28 -0400
1, 16 -- Message-ID: {react-1197671-at-jeol.com}
1, 16 -- X-Autogenerated: Reply
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1, 16 -- Subject: Re: [Microscopy] Re: 200kv TEM and field emission
1, 16 -- In-Reply-To: {200507281955.j6SJtInn006553-at-ns.microscopy.com}
1, 16 -- X-Spam-Score: (-2.82) ALL_TRUSTED
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From: bmollon-at-pacbell.net
Date: Thu, 28 Jul 2005 15:20:22 -0500
Subject: [Microscopy] rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In the U.S., the sky is always blue.

I care about people and would never use the remark of
"I dont care about you". Its a simple polite,
compassionate rule that most of us are taught to care
for people. My point was it was a poor choice of
words. Take out that sentence and I think you'll see
Nestor would still make his point without sharing his
frustrating remark of caring.

Im not going to add any remarks on the subject of this
thread. It's already contorted beyond intent of the
original author.
My posting was an observation as to how everyone,
including the sysop, got carried away with this
originally intended "amusement post" and maybe all of
us ended up being guilty of stepping on the rules.
Again, no reason to publically announce how much you
care about someone.

Anonymous posting? What more do you need besides my
email address and that Im a member of this list? Let
me know what you need to know.



--- r.sims-at-auckland.ac.nz wrote:

}
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} Dear bmollon
}
}
} How on earth do you read Nestor's email as
} "derogatory and demeaning"?
}
} Do you see some equivalence between saying "I don't
} care who you are" and
} describing someone else as practising "crap
} journalism"?
}
} What color is the sky in your world?
}
} And how about having the courage to identify
} yourself?
}
} Anonymous postings don't rate very highly with most
} people.
}
} cheers
}
} rtch
}
}
}
}
} Date sent: Wed, 27 Jul 2005 09:42:24 -0500
} To: r.sims-at-auckland.ac.nz
} X-from: bmollon-at-pacbell.net
} Send reply to: microscopy-at-microscopy.com
} Subject: [Microscopy] rules
}
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} }
} } Nestor..........since you just used a derogatory
} and
} } demeaning statement about someone publically using
} the
} } listserver........are you going to remove yourself
} } from the list? I think the "I dont care who you
} are"
} } could have been left off and your point still
} made.
} }
} } Nestor wrote:
} } "Sergy
} }
} } I don't care who you are or where your from, but I
} } insist the rules
} } which have
} } been established for over a decade are followed by
} } all. Since you
} } believe you are above the rules so be it. You are
} } welcome to
} } go elsewhere.
} }
} } I have canceled your subscription to the
} Listserver.
} }
} }
} } Nestor
} } Microscopy SysOp"
} }
} } --
} Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
} Microanalyst Fax : 64 9
} 3737435
} Department of Geology email :
} r.sims-at-auckland.ac.nz
} The University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
}
} ==============================Original
} Headers==============================
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} 14:27:30 2005
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} microscopy-at-microscopy.com
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==============================Original Headers==============================
9, 15 -- From bmollon-at-pacbell.net Thu Jul 28 15:20:22 2005
9, 15 -- Received: from web81306.mail.yahoo.com (web81306.mail.yahoo.com [206.190.37.81])
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9, 15 -- Date: Thu, 28 Jul 2005 13:20:18 -0700 (PDT)
9, 15 -- From: Bill Mollon {bmollon-at-pacbell.net}
9, 15 -- Subject: Re: [Microscopy] Re: rules
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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 15:21:16 -0500
Subject: [Microscopy] Re: rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be out of the office from July 28th returning to the office on August 8th, 2005.

==============================Original Headers==============================
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1, 16 -- From: "Patricia Corkum" {pcorkum-at-jeol.com}
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From: William.Giles-at-TIMET.com
Date: Thu, 28 Jul 2005 15:33:42 -0500
Subject: [Microscopy] Out of office replies and chat room style posts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Seems I'm being besieged by out of office messages, I'm glad your in Hawaii
but don't rub it in.

And to the chat room drama that has filled my mail box, come on guys take it
somewhere else.


*

==============================Original Headers==============================
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4, 22 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 15:34:32 -0500
Subject: [Microscopy] Re: Out of office replies and chat room style posts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be out of the office from July 28th returning to the office on August 8th, 2005.

==============================Original Headers==============================
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From: dmclea-at-sandia.gov
Date: Thu, 28 Jul 2005 15:48:52 -0500
Subject: [Microscopy] Re: Out of office replies and chat room style

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If this doesn't stop I'll never buy another JEOL product...pcorkum has
filled my inbox with automatic out of office replies! And of course,
I'll just get another one bounced form complaining! Sorry...I just had
to vent.

Dorrance McLean

-----Original Message-----
X-from: pcorkum-at-jeol.com [mailto:pcorkum-at-jeol.com]
Sent: Thursday, July 28, 2005 1:35 PM
To: McLean, Dorrance

I will be out of the office from July 28th returning to the office on
August 8th, 2005.

==============================Original
Headers==============================
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==============================Original Headers==============================
11, 31 -- From dmclea-at-sandia.gov Thu Jul 28 15:48:51 2005
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From: pcorkum-at-jeol.com
Date: Thu, 28 Jul 2005 16:29:09 -0500
Subject: [Microscopy] Re: Out of office replies and chat room style

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Thu, 28 Jul 2005 15:49:22 -0500
dmclea-at-sandia.gov wrote:
} Hello,

My most humble apologies to all!
This was a dreadful oversight on my part, and will be
corrected as promptly as possible.

I truly am sorry about the confusion.

Most cordially,
Patricia Corkum
JEOL USA
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America


==============================Original Headers==============================
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From: dbaldwin-at-dgisrd.com
Date: Thu, 28 Jul 2005 19:12:49 -0500
Subject: [Microscopy] TEM/SEM Employment Opportunity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ELECTRON MICROSCOPE TECHNICIAN - RESEARCH TRIANGLE PARK (RTP), NORTH
CAROLINA AREA

We have an immediate need for a full-time Electron Microscope Technician
with solid knowledge of maintenance and operation. 3+ years TEM and SEM
experience in the maintenance and operations of electron microscopy
required.

Send resume and salary requirements to sjeffers-at-dgisrd.com
{mailto:sjeffers-at-dgisrd.com} .



==============================Original Headers==============================
5, 20 -- From dbaldwin-at-dgisrd.com Thu Jul 28 19:12:49 2005
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5, 20 -- Subject: TEM/SEM Employment Opportunity
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From: gary-at-gaugler.com
Date: Thu, 28 Jul 2005 20:37:07 -0500
Subject: [Microscopy] Zeiss drift correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Has anyone been able to get the Zeiss/LEO drift
correction option to work? I have the license
and the dongle. Nevertheless, the feature seems
rather dysfunctional...or I don't know how to use it.

Can someone enlighten me about this option? I can
really use it for long data capture sessions combined
with the AVI capture option.

gary g.


==============================Original Headers==============================
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From: ferretinmicrowave-at-ns.microscopy.com
Date: Thu, 28 Jul 2005 21:06:22 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: How to image a snow flake

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Email: ferretinmicrowave
Name: Isabel Verde

Organization: University of Pennsylvania

Education: 9-12th Grade High School

Location: Chicago, IL, USA

Question: How do you take an image of a snowflake with an optical
microscope? What sort of special techniques and equipment do you
need in order to take the picture?

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From: ballardmark-at-gmail.com
Date: Thu, 28 Jul 2005 21:07:01 -0500
Subject: [Microscopy] AskAMicroscopist: an affordable student microscope

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Below is the result of your feedback form (NJZFM-ultra-55). It was
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---------------------------------------------------------------------------

Email: ballardmark-at-gmail.com
Name: Marcello

Organization: None

Education: 9-12th Grade High School

Location: Orlando, Florida

Question: Hi to all,

i am trying to buy the most afforable microscopy, but that will be
able to help me with biology and chemistry.

---------------------------------------------------------------------------

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From: dbaldwin-at-dgisrd.com
Date: Thu, 28 Jul 2005 21:09:44 -0500
Subject: [Microscopy] viaWWW: TEM/SEM Employment Opportunity

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Thursday, July 28, 2005 at 13:35:21
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Email: dbaldwin-at-dgisrd.com
Name: DBaldwin

Organization: BioWarn, LLC

Title-Subject: [Filtered] TEM/SEM Employment Opportunity

Question: ELECTRON MICROSCOPE TECHNICIAN - RESEARCH TRIANGLE PARK
(RTP), NORTH CAROLINA AREA

We have an immediate need for a full-time Electron Microscope
Technician with solid knowledge in maintenance and operation. 3+
years TEM and SEM experience in maintenance and operation of electron
microscopy required.

Send resume and salary requirements to: sjeffers-at-dgisrd.com.



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From: wpchan-at-u.washington.edu
Date: Thu, 28 Jul 2005 21:13:01 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Isabel

You might want to check out this site.

http://www.its.caltech.edu/~atomic/snowcrystals/photo2/photo2.htm

--
Pang (Wai Pang Chan, wpchan-at-u.washington.edu)

On Thu, 28 Jul 2005 ferretinmicrowave-at-ns.microscopy.com wrote:

} Email: ferretinmicrowave
} Name: Isabel Verde
}
} Organization: University of Pennsylvania
}
} Education: 9-12th Grade High School
}
} Location: Chicago, IL, USA
}
} Question: How do you take an image of a snowflake with an optical
} microscope? What sort of special techniques and equipment do you
} need in order to take the picture?

==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Thu, 28 Jul 2005 21:27:33 -0500
Subject: [Microscopy] Re: AskAMicroscopist: an affordable student microscope

Contents Retrieved from Microscopy Listserver Archives
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have you gone through your purchasing dept?

--- ballardmark-at-gmail.com wrote:

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} Email: ballardmark-at-gmail.com
} Name: Marcello
}
} Organization: None
}
} Education: 9-12th Grade High School
}
} Location: Orlando, Florida
}
} Question: Hi to all,
}
} i am trying to buy the most afforable microscopy,
} but that will be
} able to help me with biology and chemistry.
}
}
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__________________________________________________
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From: r.sims-at-auckland.ac.nz
Date: Fri, 29 Jul 2005 00:20:21 -0500
Subject: [Microscopy] AskAMicroscopist: an affordable student

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well said, John!

I'm sure that the enquiring high school student will find the information you
have so thoughtfully and kindly supplied to him to be useful, will encourage his
interest in microscopy, and will induce in him a new respect for microscopists.

Marcello, please wait a little longer and you may find that other list members
may post for you information even more relevant and useful than John's kind reply.

I would try, but what I know about light microscopy could easily be written on a
small Post-It note.

cheers

Ritchie Sims
Auckland
New Zealand


Quoting hoffpajo-at-yahoo.com:

}
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} }
} }
} }
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} } submitted by (ballardmark-at-gmail.com) from
} }
} http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
} }
} } on Thursday, July 28, 2005 at 10:58:06
} }
} ---------------------------------------------------------------------------
} }
} } Email: ballardmark-at-gmail.com
} } Name: Marcello
} }
} } Organization: None
} }
} } Education: 9-12th Grade High School
} }
} } Location: Orlando, Florida
} }
} } Question: Hi to all,
} }
} } i am trying to buy the most afforable microscopy,
} } but that will be
} } able to help me with biology and chemistry.
} }
} }



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From: bbuenaobra-at-nip.upd.edu.ph
Date: Fri, 29 Jul 2005 06:27:20 -0500
Subject: [Microscopy] RE: AskAMicroscopist: an affordable student

Contents Retrieved from Microscopy Listserver Archives
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Dear Isabel

Some cameras, such as the Nikon 4500 can image larger snow flakes directly
with their macro modes,
but individual crystals normally require a microscope or a powerful macro
setup.
Any stereomicroscope with camera attached will do the job, or you could
point a digital camera down the eyepiece of any suitable microscope. Either
transmitted brightfield
or darkfield illumination will work, and imaging between crossed polarisers
can generate
interesting colour effects.

The main problem is to prevent the snowflake from melting, so
the microscope slide and stage need to be at the same temperature as the
snowflake.
The simplest way, if not the most comfortable way, to do this is to work
outside in the snow-shower,
or in an unheated shed at subzero temperature.

Some tips, images and historical background can be had from this site:
http://www.its.caltech.edu/~atomic/snowcrystals/

With global warming this winter may be your last opportunity...
Wrap up warm
Chris



----- Original Message -----
X-from: {ferretinmicrowave-at-ns.microscopy.com}
To: {cjeffree-at-staffmail.ed.ac.uk}
Sent: Friday, July 29, 2005 3:06 AM


Would the Intel-Mattel microscope with a USB support be available?
That for high school could really be affordable.

Berns


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From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 29 Jul 2005 08:25:10 -0500
Subject: [Microscopy] re: An affordable student microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Check out the Surplus Shed at www.surplusshed.com. They have both
dissecting stereomicroscope and compound microscope for $ 95.00 each. I
can't vouch for their quality since I've not bought one, but the price is
right, and I'm satisfied with other things I've purchased from them. Do NOT
buy a discount chain store microscope. They're junk.

Paul Grover


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln




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From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 29 Jul 2005 08:35:59 -0500
Subject: [Microscopy] re: how to image a snowflake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Isabel,

You might want to consider making plastic snowflake replicas. Just google
'snowflake replicas' and you'll find a bunch of sites. If you have trouble
finding Formvar, contact me and I'll send you some.

I've had great fun coming inside from a snowstorm and passing these around
to guests at a party and seeing how long it takes them to figure out that
the snowflakes aren't melting indoors.

Paul Grover


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln



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From: ekman-at-itg.uiuc.edu
Date: Fri, 29 Jul 2005 08:40:06 -0500
Subject: [Microscopy] cameras in labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When I was at Florida State University the I.T. people in the Biology
Department placed networked cameras in the student computer labs.

http://www.axis.com/products/cam_210/index.htm

They had them saving a still pictures every 5 seconds to a network
server. I think you can capture video as well with the 210 which is linked
above.

Neat thing was that the large format poster printer was in view in one lab
so you could just check the web address of the camera to see if your poster
was done printing without leaving your office.

Once installed we built metal cages to protect them from being stolen.

I have no financial ties to the company above, just a product that I have
worked with in the past with good success.

Hope this helps,

Jon Ekman
Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 N. Mathews Avenue
Urbana, IL 61801 USA
Tel: 217-244-6292
Fax: 217-244-6219

-----Original Message-----
X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca]
Sent: Wednesday, July 27, 2005 1:38 PM
To: ekman-at-itg.uiuc.edu

Hi everyone,

We are currently considering adding security cameras in each of our EM
labs.

We have a very large number of users, both during working hours and
after-hours in SEM and TEM rooms. Just recently, we have had a few
incidents where people have damaged the instrument and did not come
forward to admit it.

Although we can see who logged on to the computers, you can still damage
the instrument without logging in. We believe that a CCTV in each of the
labs would be the best way to solve this problem, but unfortunately we
are faced with "privacy issues". People do not want to be watched.

Please tell me if your labs use cameras (live or log) or if you found
other ways to get around the problem.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632


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From: TindallR-at-missouri.edu
Date: Fri, 29 Jul 2005 08:46:17 -0500
Subject: [Microscopy] AskAMicroscopist: an affordable student microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Marcello,

Although I have no experience with them, I have received a catalog from
a company called Walter Products, Inc.based in Ontario. They specialize
in scientific equipment, including microscopes, telescopes, and other
supplies. Although the catalog doesn't list their prices for
microscopes, their telescope prices seem to be amazingly reasonable
(i.e., low) and their warranties seem fine.

You might want to contact them. The email is walter1-at-on.aibn.com, and
phone is 519-737-7901. Google the name to get their website. I have no
connection or experiences with them, but it might be worth a look.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







-----Original Message-----
X-from: ballardmark-at-gmail.com [mailto:ballardmark-at-gmail.com]
Sent: Thursday, July 28, 2005 9:08 PM
To: Tindall, Randy D.

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ballardmark-at-gmail.com) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Thursday, July 28, 2005 at 10:58:06
------------------------------------------------------------------------
---

Email: ballardmark-at-gmail.com
Name: Marcello

Organization: None

Education: 9-12th Grade High School

Location: Orlando, Florida

Question: Hi to all,

i am trying to buy the most afforable microscopy, but that will be able
to help me with biology and chemistry.

------------------------------------------------------------------------
---

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From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 29 Jul 2005 08:48:35 -0500
Subject: [Microscopy] snowflakes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

And be sure to check out Wilson A. Bentley's life & work. He pioneered
snowflake photography a long time ago, and did it the hard way. Amateurs
rock!

Paul Grover

------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln



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From: gstrout-at-ou.edu
Date: Fri, 29 Jul 2005 08:55:06 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow flake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Isabel,

Hava a look at snowcrystals.com it is a site with lots of info and links
about snowflakes including photography.




ferretinmicrowave-at-ns.microscopy.com wrote:

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--
--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




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From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 29 Jul 2005 09:35:57 -0500
Subject: [Microscopy] raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Esteemed Microscopists,

Since most of you apparently are in Hawaii now, I'll waste a little
bandwidth and a few KB to vent. I'd like to note that:

(1) This is a MICROSCOPY listserver. This apparently means "looking at
little things".

(2) There is apparently no requirement that one must be using the latest
multi-probe cutting edge technology, run a multi-user facility, or have a
purchasing department, federal grant, or even a job. Our common bond is
that we like to "look at little things".

(3) The greatest scientists have been, mostly, AMATEURS (from Latin
'amator', i.e. someone who does something out of love instead of for
financial gain). As we celebrate the centenary of Einstein's 'miracle year'
which changed our concept of the universe, let's remember that he did this
work as an amateur.

(4) In a few years we'll all be toothless curmudgeons and today's
schoolkids will be deciding whether Medicare covers immersion oil and cover
slips.

(5) I like to "look at little things" but I'm a carpenter by trade. If you
don't like that, bite me.

(6) If you aren't reading this it's because you're in Hawaii and I hate you
because I'm not.


Paul Grover :0)


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln




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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 10:20:16 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

very well said, and Hawaii is over rated try St
Martins, much better. you must be the one with the
Highest IQ in here.
john

--- pgrover-at-bilbo.bio.purdue.edu wrote:

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} Microscopy Society of America
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} http://www.microscopy.com/MicroscopyListserver
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}
} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll
} waste a little
} bandwidth and a few KB to vent. I'd like to note
} that:
}
} (1) This is a MICROSCOPY listserver. This
} apparently means "looking at
} little things".
}
} (2) There is apparently no requirement that one
} must be using the latest
} multi-probe cutting edge technology, run a
} multi-user facility, or have a
} purchasing department, federal grant, or even a job.
} Our common bond is
} that we like to "look at little things".
}
} (3) The greatest scientists have been, mostly,
} AMATEURS (from Latin
} 'amator', i.e. someone who does something out of
} love instead of for
} financial gain). As we celebrate the centenary of
} Einstein's 'miracle year'
} which changed our concept of the universe, let's
} remember that he did this
} work as an amateur.
}
} (4) In a few years we'll all be toothless
} curmudgeons and today's
} schoolkids will be deciding whether Medicare covers
} immersion oil and cover
} slips.
}
} (5) I like to "look at little things" but I'm a
} carpenter by trade. If you
} don't like that, bite me.
}
} (6) If you aren't reading this it's because you're
} in Hawaii and I hate you
} because I'm not.
}
}
} Paul Grover :0)
}
}
}
------------------------------------------------------------------------
} No matter how much cats fight, there always seem to
} be plenty of kittens.
} - A.
} Lincoln
}
}
}
}
} ==============================Original
} Headers==============================
} 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul
} 29 09:35:57 2005
} 15, 24 -- Received: from mailhub130.itcs.purdue.edu
} (mailhub130.itcs.purdue.edu [128.210.5.130])
} 15, 24 -- by ns.microscopy.com (8.12.11/8.12.8)
} with ESMTP id j6TEZvKW008928
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} Jul 2005 09:35:57 -0500
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} (dhcp155-220.bio.purdue.edu [128.210.155.220])
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} (8.13.4/8.13.4/internal-smtp) with ESMTP id
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} 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29
} Jul 2005 09:35:57 -0500
} 15, 24 -- From: "pgrover"
} {pgrover-at-bilbo.bio.purdue.edu}
} 15, 24 -- To: {microscopy-at-microscopy.com}
} 15, 24 -- Subject: raison d' etre
} 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500
} 15, 24 -- Message-ID:
} {000101c5944a$d5416290$dc9bd280-at-paklabpgrover}
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} 10.0.4510
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} quoted-printable to 8bit by ns.microscopy.com id
} j6TEZvKW008928
} ==============================End of -
} Headers==============================
}


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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Fri Jul 29 10:20:16 2005
5, 19 -- Received: from web50206.mail.yahoo.com (web50206.mail.yahoo.com [206.190.38.47])
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] raison d' etre
5, 19 -- To: microscopy-at-microscopy.com
5, 19 -- In-Reply-To: {200507291437.j6TEbevE011473-at-ns.microscopy.com}
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 10:20:55 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

very well said, and Hawaii is over rated try St
Martins, much better. you must be the one with the
Highest IQ in here.
john


--- pgrover-at-bilbo.bio.purdue.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll
} waste a little
} bandwidth and a few KB to vent. I'd like to note
} that:
}
} (1) This is a MICROSCOPY listserver. This
} apparently means "looking at
} little things".
}
} (2) There is apparently no requirement that one
} must be using the latest
} multi-probe cutting edge technology, run a
} multi-user facility, or have a
} purchasing department, federal grant, or even a job.
} Our common bond is
} that we like to "look at little things".
}
} (3) The greatest scientists have been, mostly,
} AMATEURS (from Latin
} 'amator', i.e. someone who does something out of
} love instead of for
} financial gain). As we celebrate the centenary of
} Einstein's 'miracle year'
} which changed our concept of the universe, let's
} remember that he did this
} work as an amateur.
}
} (4) In a few years we'll all be toothless
} curmudgeons and today's
} schoolkids will be deciding whether Medicare covers
} immersion oil and cover
} slips.
}
} (5) I like to "look at little things" but I'm a
} carpenter by trade. If you
} don't like that, bite me.
}
} (6) If you aren't reading this it's because you're
} in Hawaii and I hate you
} because I'm not.
}
}
} Paul Grover :0)
}
}
}
------------------------------------------------------------------------
} No matter how much cats fight, there always seem to
} be plenty of kittens.
} - A.
} Lincoln
}
}
}
}
} ==============================Original
} Headers==============================
} 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul
} 29 09:35:57 2005
} 15, 24 -- Received: from mailhub130.itcs.purdue.edu
} (mailhub130.itcs.purdue.edu [128.210.5.130])
} 15, 24 -- by ns.microscopy.com (8.12.11/8.12.8)
} with ESMTP id j6TEZvKW008928
} 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29
} Jul 2005 09:35:57 -0500
} 15, 24 -- Received: from paklabpgrover
} (dhcp155-220.bio.purdue.edu [128.210.155.220])
} 15, 24 -- by mailhub130.itcs.purdue.edu
} (8.13.4/8.13.4/internal-smtp) with ESMTP id
} j6TEZv5i001612
} 15, 24 -- for {microscopy-at-microscopy.com} ; Fri, 29
} Jul 2005 09:35:57 -0500
} 15, 24 -- From: "pgrover"
} {pgrover-at-bilbo.bio.purdue.edu}
} 15, 24 -- To: {microscopy-at-microscopy.com}
} 15, 24 -- Subject: raison d' etre
} 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500
} 15, 24 -- Message-ID:
} {000101c5944a$d5416290$dc9bd280-at-paklabpgrover}
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} 10.0.4510
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} 15, 24 -- Content-Transfer-Encoding: 8bit
} 15, 24 -- X-MIME-Autoconverted: from
} quoted-printable to 8bit by ns.microscopy.com id
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} ==============================End of -
} Headers==============================
}


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==============================Original Headers==============================
6, 19 -- From hoffpajo-at-yahoo.com Fri Jul 29 10:20:54 2005
6, 19 -- Received: from web50201.mail.yahoo.com (web50201.mail.yahoo.com [206.190.38.42])
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6, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 19 -- Subject: Re: [Microscopy] raison d' etre
6, 19 -- To: microscopy-at-microscopy.com
6, 19 -- In-Reply-To: {200507291437.j6TEbevE011473-at-ns.microscopy.com}
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From: microbill-at-mohawk.net
Date: Fri, 29 Jul 2005 10:37:48 -0500
Subject: [Microscopy] raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I fully concur - I'll be there next week

At 11:21 AM 7/29/2005, you wrote:



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==============================Original Headers==============================
7, 20 -- From microbill-at-mohawk.net Fri Jul 29 10:37:48 2005
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7, 20 -- Date: Fri, 29 Jul 2005 11:37:33 -0400
7, 20 -- From: Bill Miller {microbill-at-mohawk.net}
7, 20 -- Subject: Re: [Microscopy] Re: raison d' etre
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==============================End of - Headers==============================




From: frank.karl-at-degussa.com
Date: Fri, 29 Jul 2005 10:38:10 -0500
Subject: [Microscopy] Fw: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





I find myself perplexed by the reason for Paul Gover’s note. I typically
check titles and skim the content of the note if I’m interested.
Everything else gets trashed canned, so I haven’t been paying too much
attention to the discussion. If my lurking finds a theme I’m interested
in, I’m not above kicking the embers to bring forth light and heat. With
this in mind….


I’ve been making a reasonable living as a microscopist for over 25 years
and I still consider myself a very fortunate amateur. I too like to look
at little things and to that end built a home microscopy lab while I was in
college. I consider the time spent studying diatoms, collecting pollen or
cutting free hand thin section of multi-layer bottles to be golden. I find
that these skills are transferable to the professional arena.

My first position in a multi-used environment was in the tire industry.
Conversations over coffee revealed none of my new co-workers owned a home
lab. My microscopy culture tells me all microscopists have a home lab. I
knew, at that moment, my co-workers were simply employees and nothing more.
All of the microscopist I admire have a home lab and several have created
an employment and provided for the welfare of their family from it. Some
find their professional lives do not provide the luxury of using the home
lab and most regret it.

If amateurs are not welcome on this list, I suggest a name change. I have
found this server a useful tool, but I’ll hate to see it restricted to only
“profession†microscopist.

Frank Karl
Degussa Corporation


.
----- Forwarded by Frank Karl/AKR/Degussa-Huels/US on 07/29/2005 11:34 AM
-----

pgrover-at-bilbo.bio
.purdue.edu To: frank.karl-at-degussa.com
cc:
07/29/2005 10:37 Subject: [Microscopy] raison d' etre
AM
Please respond to
microscopy








----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Esteemed Microscopists,

Since most of you apparently are in Hawaii now, I'll waste a little
bandwidth and a few KB to vent. I'd like to note that:

(1) This is a MICROSCOPY listserver. This apparently means "looking at
little things".

(2) There is apparently no requirement that one must be using the latest
multi-probe cutting edge technology, run a multi-user facility, or have a
purchasing department, federal grant, or even a job. Our common bond is
that we like to "look at little things".

(3) The greatest scientists have been, mostly, AMATEURS (from Latin
'amator', i.e. someone who does something out of love instead of for
financial gain). As we celebrate the centenary of Einstein's 'miracle
year'
which changed our concept of the universe, let's remember that he did this
work as an amateur.

(4) In a few years we'll all be toothless curmudgeons and today's
schoolkids will be deciding whether Medicare covers immersion oil and cover
slips.

(5) I like to "look at little things" but I'm a carpenter by trade. If
you
don't like that, bite me.

(6) If you aren't reading this it's because you're in Hawaii and I hate
you
because I'm not.


Paul Grover :0)


------------------------------------------------------------------------
No matter how much cats fight, there always seem to be plenty of kittens.
- A. Lincoln






==============================Original Headers==============================
38, 19 -- From frank.karl-at-degussa.com Fri Jul 29 10:38:10 2005
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 10:46:36 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

enjoy it is a beautiful island where everyone is very
friendly. i may move there full time if i can get the
right price for my house
john

--- microbill-at-mohawk.net wrote:

}
}
}
}
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} Microscopy Society of America
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}
} I fully concur - I'll be there next week
}
} At 11:21 AM 7/29/2005, you wrote:
}
}
}
}
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
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}
} ----------------------------------------------------------------------------
} }
} } very well said, and Hawaii is over rated try St
} } Martins, much better. you must be the one with the
} } Highest IQ in here.
} } john
} }
} }
} } --- pgrover-at-bilbo.bio.purdue.edu wrote:
} }
} } }
} } }
} } }
} } }
}
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } }
}
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
}
} ----------------------------------------------------------------------------
} } }
} } } Esteemed Microscopists,
} } }
} } } Since most of you apparently are in Hawaii now,
} I'll
} } } waste a little
} } } bandwidth and a few KB to vent. I'd like to
} note
} } } that:
} } }
} } } (1) This is a MICROSCOPY listserver. This
} } } apparently means "looking at
} } } little things".
} } }
} } } (2) There is apparently no requirement that one
} } } must be using the latest
} } } multi-probe cutting edge technology, run a
} } } multi-user facility, or have a
} } } purchasing department, federal grant, or even a
} job.
} } } Our common bond is
} } } that we like to "look at little things".
} } }
} } } (3) The greatest scientists have been, mostly,
} } } AMATEURS (from Latin
} } } 'amator', i.e. someone who does something out of
} } } love instead of for
} } } financial gain). As we celebrate the centenary
} of
} } } Einstein's 'miracle year'
} } } which changed our concept of the universe, let's
} } } remember that he did this
} } } work as an amateur.
} } }
} } } (4) In a few years we'll all be toothless
} } } curmudgeons and today's
} } } schoolkids will be deciding whether Medicare
} covers
} } } immersion oil and cover
} } } slips.
} } }
} } } (5) I like to "look at little things" but I'm a
} } } carpenter by trade. If you
} } } don't like that, bite me.
} } }
} } } (6) If you aren't reading this it's because
} you're
} } } in Hawaii and I hate you
} } } because I'm not.
} } }
} } }
} } } Paul Grover :0)
} } }
} } }
} } }
}
} ------------------------------------------------------------------------
} } } No matter how much cats fight, there always seem
} to
} } } be plenty of kittens.
} } } -
} A.
} } } Lincoln
} } }
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri
} Jul
} } } 29 09:35:57 2005
} } } 15, 24 -- Received: from
} mailhub130.itcs.purdue.edu
} } } (mailhub130.itcs.purdue.edu [128.210.5.130])
} } } 15, 24 -- by ns.microscopy.com
} (8.12.11/8.12.8)
} } } with ESMTP id j6TEZvKW008928
} } } 15, 24 -- for {microscopy-at-microscopy.com} ;
} Fri, 29
} } } Jul 2005 09:35:57 -0500
} } } 15, 24 -- Received: from paklabpgrover
} } } (dhcp155-220.bio.purdue.edu [128.210.155.220])
} } } 15, 24 -- by mailhub130.itcs.purdue.edu
} } } (8.13.4/8.13.4/internal-smtp) with ESMTP id
} } } j6TEZv5i001612
} } } 15, 24 -- for {microscopy-at-microscopy.com} ;
} Fri, 29
} } } Jul 2005 09:35:57 -0500
} } } 15, 24 -- From: "pgrover"
} } } {pgrover-at-bilbo.bio.purdue.edu}
} } } 15, 24 -- To: {microscopy-at-microscopy.com}
} } } 15, 24 -- Subject: raison d' etre
} } } 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] raison d' etre
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From: marc.pypaert-at-yale.edu
Date: Fri, 29 Jul 2005 10:49:02 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Paul,

Why perpetuate the myth of the amateur scientist doing his job just for
the love of science and not for money? As far as I know, Einstein made
a pretty good living for himself while teaching at Princeton, and I'm
sure
that great scientists before him, like Louis Pasteur, were not poor
either!
I like many others on this listserver have chosen microscopy as a
career,
as a way to make a living, and the fact that the "little things" that
we look
at every day are so beautiful is just a bonus to our jobs! We're doing
science a disservice if we tell schoolkids that, if they want to make a
living, they should not consider a scientific career!

But like you, I'm pretty jealous of all those going to Hawaii this
weekend!

Marc


On Friday, July 29, 2005, at 10:37 AM, pgrover-at-bilbo.bio.purdue.edu
wrote:

}
}
}
} -----------------------------------------------------------------------
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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
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} -----
}
} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll waste a little
} bandwidth and a few KB to vent. I'd like to note that:
}
} (1) This is a MICROSCOPY listserver. This apparently means "looking
} at
} little things".
}
} (2) There is apparently no requirement that one must be using the
} latest
} multi-probe cutting edge technology, run a multi-user facility, or
} have a
} purchasing department, federal grant, or even a job. Our common bond
} is
} that we like to "look at little things".
}
} (3) The greatest scientists have been, mostly, AMATEURS (from Latin
} 'amator', i.e. someone who does something out of love instead of for
} financial gain). As we celebrate the centenary of Einstein's 'miracle
} year'
} which changed our concept of the universe, let's remember that he did
} this
} work as an amateur.
}
} (4) In a few years we'll all be toothless curmudgeons and today's
} schoolkids will be deciding whether Medicare covers immersion oil and
} cover
} slips.
}
} (5) I like to "look at little things" but I'm a carpenter by trade.
} If you
} don't like that, bite me.
}
} (6) If you aren't reading this it's because you're in Hawaii and I
} hate you
} because I'm not.
}
}
} Paul Grover :0)
}
}
} -----------------------------------------------------------------------
} -
} No matter how much cats fight, there always seem to be plenty of
} kittens.
} - A. Lincoln
}
}
}
}
} ==============================Original
} Headers==============================
} 15, 24 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 09:35:57 2005
} 15, 24 -- Received: from mailhub130.itcs.purdue.edu
} (mailhub130.itcs.purdue.edu [128.210.5.130])
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} j6TEZvKW008928
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} -0500
} 15, 24 -- Received: from paklabpgrover (dhcp155-220.bio.purdue.edu
} [128.210.155.220])
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} with ESMTP id j6TEZv5i001612
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} -0500
} 15, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu}
} 15, 24 -- To: {microscopy-at-microscopy.com}
} 15, 24 -- Subject: raison d' etre
} 15, 24 -- Date: Fri, 29 Jul 2005 09:35:57 -0500
} 15, 24 -- Message-ID: {000101c5944a$d5416290$dc9bd280-at-paklabpgrover}
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}
}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original Headers==============================
9, 18 -- From marc.pypaert-at-yale.edu Fri Jul 29 10:49:02 2005
9, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu [130.132.232.48])
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9, 18 -- Date: Fri, 29 Jul 2005 11:45:25 -0400
9, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
9, 18 -- Subject: Re: [Microscopy] raison d' etre
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 10:50:55 -0500
Subject: [Microscopy] Re: Fw: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

well to be honest, most of us have a life after work,
and after 25 years of 12 hour days it gets a little
old.
you know just upkeeping our lives can be a full time
job, not trying to kick anything up either.
which is one of the reasons i retired early.

--- frank.karl-at-degussa.com wrote:

}
}
}
}
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} Microscopy Society of America
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}
}
}
}
}
} I find myself perplexed by the reason for Paul
} Gover’s note. I typically
} check titles and skim the content of the note if
} I’m interested.
} Everything else gets trashed canned, so I haven’t
} been paying too much
} attention to the discussion. If my lurking finds a
} theme I’m interested
} in, I’m not above kicking the embers to bring
} forth light and heat. With
} this in mind….
}
}
} I’ve been making a reasonable living as a
} microscopist for over 25 years
} and I still consider myself a very fortunate
} amateur. I too like to look
} at little things and to that end built a home
} microscopy lab while I was in
} college. I consider the time spent studying
} diatoms, collecting pollen or
} cutting free hand thin section of multi-layer
} bottles to be golden. I find
} that these skills are transferable to the
} professional arena.
}
} My first position in a multi-used environment was in
} the tire industry.
} Conversations over coffee revealed none of my new
} co-workers owned a home
} lab. My microscopy culture tells me all
} microscopists have a home lab. I
} knew, at that moment, my co-workers were simply
} employees and nothing more.
} All of the microscopist I admire have a home lab and
} several have created
} an employment and provided for the welfare of their
} family from it. Some
} find their professional lives do not provide the
} luxury of using the home
} lab and most regret it.
}
} If amateurs are not welcome on this list, I suggest
} a name change. I have
} found this server a useful tool, but I’ll hate to
} see it restricted to only
} “profession†microscopist.
}
} Frank Karl
} Degussa Corporation
}
}
} .
} ----- Forwarded by Frank Karl/AKR/Degussa-Huels/US
} on 07/29/2005 11:34 AM
} -----
}
}
}
} pgrover-at-bilbo.bio
}
}
} .purdue.edu To:
} frank.karl-at-degussa.com
}
} cc:
}
}
} 07/29/2005 10:37
} Subject: [Microscopy] raison d' etre
}
} AM
}
}
} Please respond to
}
}
} microscopy
}
}
}
}
}
}
}
}
}
}
}
}
}
----------------------------------------------------------------------------
}
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
}
} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll
} waste a little
} bandwidth and a few KB to vent. I'd like to note
} that:
}
} (1) This is a MICROSCOPY listserver. This
} apparently means "looking at
} little things".
}
} (2) There is apparently no requirement that one
} must be using the latest
} multi-probe cutting edge technology, run a
} multi-user facility, or have a
} purchasing department, federal grant, or even a job.
} Our common bond is
} that we like to "look at little things".
}
} (3) The greatest scientists have been, mostly,
} AMATEURS (from Latin
} 'amator', i.e. someone who does something out of
} love instead of for
} financial gain). As we celebrate the centenary of
} Einstein's 'miracle
} year'
} which changed our concept of the universe, let's
} remember that he did this
} work as an amateur.
}
} (4) In a few years we'll all be toothless
} curmudgeons and today's
} schoolkids will be deciding whether Medicare covers
} immersion oil and cover
} slips.
}
} (5) I like to "look at little things" but I'm a
} carpenter by trade. If
} you
} don't like that, bite me.
}
} (6) If you aren't reading this it's because you're
} in Hawaii and I hate
} you
} because I'm not.
}
}
} Paul Grover :0)
}
}
}
------------------------------------------------------------------------
} No matter how much cats fight, there always seem to
} be plenty of kittens.
} - A.
} Lincoln
}
}
}
}
}
}
} ==============================Original
} Headers==============================
} 38, 19 -- From frank.karl-at-degussa.com Fri Jul 29
} 10:38:10 2005
} 38, 19 -- Received: from framailout1.rz.itson.com
} (mailout2.degussa.com [149.216.91.173])
} 38, 19 -- by ns.microscopy.com (8.12.11/8.12.8)
} with ESMTP id j6TFc49t000367
} 38, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri,
} 29 Jul 2005 10:38:10 -0500
} 38, 19 -- Received: from
} mobuscomm01.mail.degussa.com ([172.20.6.74])
} 38, 19 -- by framailout1.rz.itson.com
} (8.13.3/8.13.3/Debian-6) with ESMTP id
} j6TFapLj006130
} 38, 19 -- for {microscopy-at-msa.microscopy.com} ; Fri,
} 29 Jul 2005 17:37:17 +0200
} 38, 19 -- Subject: Fw: [Microscopy] raison d' etre
} 38, 19 -- To: microscopy-at-msa.microscopy.com
} 38, 19 -- X-Mailer: Lotus Notes Release 6.5.2 June
} 01, 2004
} 38, 19 -- Message-ID:
}
{OFA5E2B331.F5B85357-ON8525704D.005583B8-8525704D.0055C455-at-degussa.com}
}
=== message truncated ===


__________________________________________________
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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Fri Jul 29 10:50:55 2005
5, 19 -- Received: from web50203.mail.yahoo.com (web50203.mail.yahoo.com [206.190.38.44])
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5, 19 -- Date: Fri, 29 Jul 2005 08:50:54 -0700 (PDT)
5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] Fw: raison d' etre
5, 19 -- To: microscopy-at-microscopy.com
5, 19 -- In-Reply-To: {200507291539.j6TFdf7p005133-at-ns.microscopy.com}
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 11:07:34 -0500
Subject: [Microscopy] Re: AskAMicroscopist: an affordable student

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

you do have a sense of humor after all, i was begining
to wonder. what a nice sardonic reply.
finlly get it i do have a name huh?

--- r.sims-at-auckland.ac.nz wrote:

}
}
}
}
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} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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}
}
} Well said, John!
}
} I'm sure that the enquiring high school student will
} find the information you
} have so thoughtfully and kindly supplied to him to
} be useful, will encourage his
} interest in microscopy, and will induce in him a new
} respect for microscopists.
}
} Marcello, please wait a little longer and you may
} find that other list members
} may post for you information even more relevant and
} useful than John's kind reply.
}
} I would try, but what I know about light microscopy
} could easily be written on a
} small Post-It note.
}
} cheers
}
} Ritchie Sims
} Auckland
} New Zealand
}
}
} Quoting hoffpajo-at-yahoo.com:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
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} Microscopy Society of America
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} }
} } have you gone through your purchasing dept?
} }
} } --- ballardmark-at-gmail.com wrote:
} }
} } }
} } }
} } }
} } }
} }
}
----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
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} } }
} }
}
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} } }
} }
}
----------------------------------------------------------------------------
} } }
} } } Below is the result of your feedback form
} } } (NJZFM-ultra-55). It was
} } } submitted by (ballardmark-at-gmail.com) from
} } }
} }
}
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
} } }
} } } on Thursday, July 28, 2005 at 10:58:06
} } }
} }
}
---------------------------------------------------------------------------
} } }
} } } Email: ballardmark-at-gmail.com
} } } Name: Marcello
} } }
} } } Organization: None
} } }
} } } Education: 9-12th Grade High School
} } }
} } } Location: Orlando, Florida
} } }
} } } Question: Hi to all,
} } }
} } } i am trying to buy the most afforable
} microscopy,
} } } but that will be
} } } able to help me with biology and chemistry.
} } }
} } }
}
}
}
} -------------------------------------------------
} This mail sent through University of Auckland
} http://www.auckland.ac.nz/
}
} ==============================Original
} Headers==============================
} 13, 35 -- From r.sims-at-auckland.ac.nz Fri Jul 29
} 00:20:20 2005
} 13, 35 -- Received: from smtpc.itss.auckland.ac.nz
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} Jul 2005 17:20:18 +1200 (NZST)
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From: hoffpajo-at-yahoo.com
Date: Fri, 29 Jul 2005 11:13:51 -0500
Subject: [Microscopy] Re: snowflakes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

wasn't there an article or something in the
Smithsonian a little while back? i will have to check
my back issues.


--- pgrover-at-bilbo.bio.purdue.edu wrote:

}
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} And be sure to check out Wilson A. Bentley's life &
} work. He pioneered
} snowflake photography a long time ago, and did it
} the hard way. Amateurs
} rock!
}
} Paul Grover
}
}
------------------------------------------------------------------------
} No matter how much cats fight, there always seem to
} be plenty of kittens.
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From: cammer-at-aecom.yu.edu
Date: Fri, 29 Jul 2005 11:32:11 -0500
Subject: [Microscopy] Re: snowflakes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

more "amateur" snowflake pics, 6 web pages beginning at
http://cammer.net/blog/snowflake.htm and ending with a movie at
http://cammer.net/blog/snowflake06.htm

Also a favorite at
http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm





At 08:49 AM 07/29/05 -0500, you wrote:



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____________________________________________________________________________
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**This electronic transmission contains information that is privileged.**


==============================Original Headers==============================
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From: Elliott-at-arizona.edu
Date: Fri, 29 Jul 2005 11:44:03 -0500
Subject: [Microscopy] snowflakes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How about

http://emu.arsusda.gov/snowsite/default.html


David


On Jul 29, 2005, at 9:34 AM, cammer-at-aecom.yu.edu wrote:

}
}
}
} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
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} http://www.microscopy.com/MicroscopyListserver
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}
} more "amateur" snowflake pics, 6 web pages beginning at
} http://cammer.net/blog/snowflake.htm and ending with a movie at
} http://cammer.net/blog/snowflake06.htm
}
} Also a favorite at
} http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm
}
}
}
}
}
} At 08:49 AM 07/29/05 -0500, you wrote:
}
}
}
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ------
} }
} } And be sure to check out Wilson A. Bentley's life & work. He
} } pioneered
} } snowflake photography a long time ago, and did it the hard way.
} } Amateurs
} } rock!
} }
} } Paul Grover
} }
} } ----------------------------------------------------------------------
} } --
} } No matter how much cats fight, there always seem to be plenty of
} } kittens.
} } - A. Lincoln
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 5, 23 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul 29 08:48:35 2005
} } 5, 23 -- Received: from mailhub130.itcs.purdue.edu
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} } 5, 23 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu}
} } 5, 23 -- To: {microscopy-at-microscopy.com}
} } 5, 23 -- Subject: snowflakes
} } 5, 23 -- Date: Fri, 29 Jul 2005 08:48:35 -0500
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}
} _______________________________________________________________________
} _____
} Michael Cammer Analytical Imaging Facility Albert Einstein Coll.
} of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY
} 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
} **This electronic transmission contains information that is
} privileged.**
}
}
} ==============================Original
} Headers==============================
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From: tivol-at-caltech.edu
Date: Fri, 29 Jul 2005 12:13:31 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow flake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Jul 28, 2005, at 7:06 PM, ferretinmicrowave-at-ns.microscopy.com wrote:

} Question: How do you take an image of a snowflake with an optical
} microscope? What sort of special techniques and equipment do you
} need in order to take the picture?
}
Dear Isabel,
There is a wonderful book by Dr. Ken Libbrecht called The Snowflake,
which has some information about how the incredible images in the book
were taken. Basically, he took a microscope slide to collect the
flakes as they fell and kept everything cold while providing
appropriate illumination for microphotography. If you need details not
provided in the book, I'm pretty sure that Ken would be willing to help
you out. I can give you his contact info off-list if you want.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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5, 27 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: How to image a snow flake
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From: msimms-at-tracelabs.com
Date: Fri, 29 Jul 2005 12:49:28 -0500
Subject: [Microscopy] viaWWW: Measurement of ink depth/contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (msimms-at-tracelabs.com) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Friday, July 29, 2005 at 10:06:10
---------------------------------------------------------------------------

Email: msimms-at-tracelabs.com
Name: Michael Simms

Organization: Trace Laboratories - Central

Title-Subject: [Filtered] Measurement of ink depth/contrast

Question: Hello Gentlemen,
I work for an independent testing organization, Trace Laboratories - Central.
We have been asked to have printing ink measured for depth and
contrast. Laser printing is done on the insulation sleeve of a cable.
per Sikorsky specification SS7333, Paragraph 4.6.5
This asks for measuring to an accuracy of 0.0001 inch with a contour
projector or calibrated microscope or Zygo measuring device. The
contrast measurement is suggested to be made with a Spectrum
Technology CMS^2 Contrast Measuring System. I would need evidence of
either an accredited laboratory or calibration to pass on to the
customer.

Is this something that someone associated with this resource might be
able to quote?

Regards,
Mike

Mike Simms
Chemist
Trace Laboratories - Central
1150 W. Euclid Ave.
Palatine, IL 60067

phone 847-934-5300
fax 847-934-4600
www.tracelabs.com



---------------------------------------------------------------------------

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From: aetmicro-at-optonline.net
Date: Fri, 29 Jul 2005 15:10:04 -0500
Subject: [Microscopy] viaWWW: unstable beam EM300

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
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---------------------------------------------------------------------------

Email: aetmicro-at-optonline.net
Name: andrew thelian

Organization: nanoprobes, inc

Title-Subject: [Filtered] MListserver: unstable beam

Question: Hi,

I have been having a problem with my beam stability currently... I
operate a Philips EM300... and in an attempt to regain stability i
cleaned the contacts of the high tension line that runs from the gun
to the high voltage tank... (the cleaning included the insulators as
well)

My questions are... What kind of oil do I use inside the insulators?
(i was advised to use Santovac 5 and would like verification because
of its cost) What kind of oil should I use for the High Voltage Tank?

Any links to locate vendors would be of great help.

Thank You,
Andy

---------------------------------------------------------------------------

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From: derby-at-nmt.edu
Date: Fri, 29 Jul 2005 17:00:34 -0500
Subject: [Microscopy] Re: viaWWW: unstable beam EM300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

on 7/29/05 2:14 PM, aetmicro-at-optonline.net at aetmicro-at-optonline.net wrote:

} Santovac 5

The Santovac oil is for the diffusion pump *NOT* the high tension tank.
I use to know that info. but it has gone poof.
I would call FEI they took over Philips or the changed their name they would
know.

Robert Derby
New Mexico Tech.



==============================Original Headers==============================
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From: aetmicro-at-optonline.net
Date: Sat, 30 Jul 2005 07:15:00 -0500
Subject: [Microscopy] viaWWW: unstable beam EM300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I read that as well... being that Santovac 5 is made for diffusion pumps...
I wasn't going to use it for the high voltage tank... but for the high
tension line insulators from the gun to the tank...

I believe its extremely low water content is what makes it a good choice for
the insulators... to prevent arcing...

Thank you for your help I am very appreciative:),
Andrew

--
No virus found in this outgoing message.
Checked by AVG Anti-Virus.
Version: 7.0.338 / Virus Database: 267.9.5/58 - Release Date: 7/25/2005



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From: kgmnrc-at-hotmail.com
Date: Sat, 30 Jul 2005 07:51:15 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Bill:
A very complete theses on how to do it and how it was done please see
Historical Microsopical Society of Canada Bulletin Volume 14 No 55 February
2003 Most of the paper dealt with snow crystals / snow flakes. Alas the
Historical Microsopical Society of Canada is no more it is part of the
Microscope Historical Society.
Hope this helps.
Regards,
Keith

-----Original Message-----
X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
Sent: Friday, July 29, 2005 1:17 PM
To: kgmnrc-at-hotmail.com


On Jul 28, 2005, at 7:06 PM, ferretinmicrowave-at-ns.microscopy.com wrote:

} Question: How do you take an image of a snowflake with an optical
} microscope? What sort of special techniques and equipment do you
} need in order to take the picture?
}
Dear Isabel,
There is a wonderful book by Dr. Ken Libbrecht called The Snowflake,

which has some information about how the incredible images in the book
were taken. Basically, he took a microscope slide to collect the
flakes as they fell and kept everything cold while providing
appropriate illumination for microphotography. If you need details not
provided in the book, I'm pretty sure that Ken would be willing to help
you out. I can give you his contact info off-list if you want.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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a snow flake
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From: r.sims-at-auckland.ac.nz
Date: Sat, 30 Jul 2005 15:46:43 -0500
Subject: [Microscopy] Santovac for insulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suspect that transformer oil might do a better job as well as costing far less.

But FEI will have good advice on this, anyway.

cheers

rtch

}
} Hi,
}
} I read that as well... being that Santovac 5 is made for diffusion
} pumps... I wasn't going to use it for the high voltage tank... but for
} the high tension line insulators from the gun to the tank...
}
} I believe its extremely low water content is what makes it a good
} choice for the insulators... to prevent arcing...
}
} Thank you for your help I am very appreciative:),
} Andrew
}


--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


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From: stefan.diller-at-t-online.de
Date: Sat, 30 Jul 2005 18:02:47 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Reichert Histostat manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (stefan.diller-at-t-online.de) from
http://microscopy.com/MLFormMail.html on Saturday, July 30, 2005 at
13:43:48
---------------------------------------------------------------------------

Email: stefan.diller-at-t-online.de
Name: Stefan Diller

Title-Subject: [Filtered] Reichert Histostat 8035 Paraffin Embedder
manual needed

Question: Dear members,
I am looking urgently for a user manual and if possible a service
manual or the electronic layout for the Reichert Histostat Paraffin
Embedder Modell 8035.

Best regards,
Stefan Diller


---------------------------------------------------------------------------

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From: hoffpajo-at-yahoo.com
Date: Sat, 30 Jul 2005 19:13:31 -0500
Subject: [Microscopy] Re: viaWWW: unstable beam EM300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

it's probly been over 15 years since i used a 300. but
you might want to contact FEI at www.feic.com. if you
contact me off line i can give you an email address of
someone to talk to directly at FEI.
john

--- aetmicro-at-optonline.net wrote:

}
}
}
}
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} Below is the result of your feedback form
} (NJZFM-ultra-55). It was
} submitted by (aetmicro-at-optonline.net) from
}
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} on
} Friday, July 29, 2005 at 13:07:28
}
---------------------------------------------------------------------------
}
} Email: aetmicro-at-optonline.net
} Name: andrew thelian
}
} Organization: nanoprobes, inc
}
} Title-Subject: [Filtered] MListserver: unstable beam
}
} Question: Hi,
}
} I have been having a problem with my beam stability
} currently... I
} operate a Philips EM300... and in an attempt to
} regain stability i
} cleaned the contacts of the high tension line that
} runs from the gun
} to the high voltage tank... (the cleaning included
} the insulators as
} well)
}
} My questions are... What kind of oil do I use inside
} the insulators?
} (i was advised to use Santovac 5 and would like
} verification because
} of its cost) What kind of oil should I use for the
} High Voltage Tank?
}
} Any links to locate vendors would be of great help.
}
} Thank You,
} Andy
}
}
---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Sat, 30 Jul 2005 19:20:35 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: How to image a snow flake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

here is a big a list of web sites as i can compile
Snow from eduScapes 42eXplore
http://eduscapes.com/42explore/snow.htm
Make Snowflakes from KinderArt
http://www.kinderart.com/seasons/dec7.shtml
Snow
http://www.muohio.edu/dragonfly/snow/snow.HTMLX
Snow facts
http://tqjunior.advanced.org/3876/snowfacts.html
Old Camera Photos
http://www.flash.net/~bobgil/cam/camera.html
Make Your Own Virtual Snowflake
http://www.muohio.edu/dragonfly/snow/icensnow.htmlx
Edible Snowflakes Recipe
http://www.stepbystepcc.com/holidays/christmas5.html
Scientists See Snowflakes
http://www.ars.usda.gov/is/kids/environment/story2/snowflakeframes.htm

Snowflakes information
http://www.macatawa.org/~oias/snowflak.htm
Using a Microscope for Snowflakes
http://www.microscopy-uk.org.uk/mag/artfeb00/eksnow.html

Wilson A. Bentley --The Snowflake Man
http://www.snowflakebentley.com/
Pictures of His Snowflakes
http://www.snowflakebentley.com/snowflakes.htm
Life of Wilson Bentley (middle grades)
http://www.virtualvermont.com/history/sbentley.html
Jericho, Vermont
http://www.jericho-underhill.com/
Famous People from Vermont
http://www.virtualvermont.com/history/people.html
Photography from eduScapes 42eXplore
http://eduscapes.com/42explore/photog.htm

Educator Links
Story of Wilson Bentley Life (Upper level reading)
http://www.snowflakebentley.com/sfman.htm
http://www.snowflakebentley.com/prior.html
Photographing Snowflakes (Upper level reading)
http://www.snowflakebentley.com/wbsf.htm
Snowflakes- A Thematic Approach
http://www.wsanford.com/~wsanford/exo/n-m_snowflakes.html

Further Reading About Ice and Snow
http://www.muohio.edu/dragonfly/snow/readings.HTMLX
Making a Snowflake (Upper level reading)
http://highhopes.com/snowflakes.html
History of Photography (Mature/adult)
http://home.globalcrossing.net/~sos/history.html
History of Photography (Mature/adult)
http://www.rleggat.com/photohistory/
Snowflake Bentley
http://www.carolhurst.com/titles/snowflakebentley.html

Suddenly Snow--Snowflake Activities
http://www.earthwalk.com/techwize/volume1/january/JANLESSON.HTML




--- kgmnrc-at-hotmail.com wrote:

}
}
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}
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} Dear Bill:
} A very complete theses on how to do it and how it
} was done please see
} Historical Microsopical Society of Canada Bulletin
} Volume 14 No 55 February
} 2003 Most of the paper dealt with snow crystals /
} snow flakes. Alas the
} Historical Microsopical Society of Canada is no more
} it is part of the
} Microscope Historical Society.
} Hope this helps.
} Regards,
} Keith
}
} -----Original Message-----
} X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu]
}
} Sent: Friday, July 29, 2005 1:17 PM
} To: kgmnrc-at-hotmail.com
} Subject: [Microscopy] Re: [Filtered]
} AskAMicroscopist: How to image a snow
} flake
}
}
}
}
}
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}
} On Jul 28, 2005, at 7:06 PM,
} ferretinmicrowave-at-ns.microscopy.com wrote:
}
} } Question: How do you take an image of a snowflake
} with an optical
} } microscope? What sort of special techniques and
} equipment do you
} } need in order to take the picture?
} }
} Dear Isabel,
} There is a wonderful book by Dr. Ken Libbrecht
} called The Snowflake,
}
} which has some information about how the incredible
} images in the book
} were taken. Basically, he took a microscope slide
} to collect the
} flakes as they fell and kept everything cold while
} providing
} appropriate illumination for microphotography. If
} you need details not
} provided in the book, I'm pretty sure that Ken would
} be willing to help
} you out. I can give you his contact info off-list
} if you want.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}
} ==============================Original
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} 5, 27 -- Subject: Re: [Microscopy] [Filtered]
} AskAMicroscopist: How to image
} a snow flake
} 5, 27 -- Date: Fri, 29 Jul 2005 10:14:29 -0700
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From: frah0010-at-tc.umn.edu
Date: Sat, 30 Jul 2005 21:21:50 -0500
Subject: [Microscopy] question: JEOL 8900 control

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Microscopy folks,

First, apologies for cross-posting with the Microbeam Analysis
Society...

Second, apologies for sending everyone a question with such a narrow
focus...

I manage the University of Minnesota's Electron Microprobe Lab, and
we have a JEOL JXA-8900R. I am looking to replace our original 1994-
vintage HP workstation with something newer and more reliable. I am
investigating our options, including just a new HP workstation and
switching to the "Probe for Windows" software. I know that my
supervisor and the department head will ask if I have checked into
every option. In particular, I anticipate they will ask me about the
possibility of running the JEOL software on a PC running Linux rather
than a HP workstation running HP-UX. Has anyone ever tried this?
Would it work? Wouldn't it? I suspect that it wouldn't fully
function, but I'd like to be able to speak to the issue with more
information than just my guess. Any information or opinions are most
welcome.

Thanks,
Ellery

--------------------
Ellery E. Frahm
Research Scientist/Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu


==============================Original Headers==============================
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7, 15 -- To: Microscopy-at-Microscopy.Com
7, 15 -- From: Ellery Frahm {frah0010-at-tc.umn.edu}
7, 15 -- Subject: question: JEOL 8900 control
7, 15 -- Date: Sat, 30 Jul 2005 21:21:48 -0500
7, 15 -- X-Mailer: Apple Mail (2.733)
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From: luc-at-anaspec.co.za
Date: Sat, 30 Jul 2005 15:52:08 -0500
Subject: [Microscopy] Re: Santovac for insulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

No no stop! Dont try the transformer oil on the gun part. Transformer oil eats plastics and rubbers. Been there done that and paid the price. If you want to use any oils in the gun part of a EM use any of the silicone oils from dow corning. 702 or 705. that works just as well. What we use is the insulating epoxy they use for electrical cables. You can buy it from your local electrician shop. It comes in a packet with two sections. simply mix the two halfs, pour into the gun HT cable connection area, after repair, and it's fine. Much cheaper, less messy and safer.


---------- Original Message ----------------------------------
X-from: r.sims-at-auckland.ac.nz
Reply-To: microscopy-at-microscopy.com

--
Luc Harmsen
ANASPEC South Africa www.anaspec.co.za
Tel: +27 11 794 8340
Fax: +27 11 794 8349
P.O. Box 2561
Honeydew 2040
Gauteng, South Africa

--

==============================Original Headers==============================
6, 13 -- From luc-at-anaspec.co.za Mon Aug 1 04:49:54 2005
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From: istocks-at-CLEMSON.EDU
Date: Mon, 1 Aug 2005 08:23:22 -0500
Subject: [Microscopy] LM- embedding insect cuticle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Andy

To save writing out all the options please take a look at our web site HINTS
and TIPS to check where your problem is,
is it the gun, gun chamber, cable or high voltage tank? Do not consider
that the only problem is likely to be the insulation of the gun. Chamber
cleanliness, vacuum level etc all play a part in high voltage breakdown a
feature that will result in beam instability.

But is it beam instability due to the gun (emission meter variations,
virtual source fluttering) or due to contamination in the condenser system
illumination flutter or movement)?

Hope this helps?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel +44 1280 814774 Direct Line 816512 Fax 814007
www.emcourses.com



----- Original Message -----
X-from: {aetmicro-at-optonline.net}
To: {protrain-at-emcourses.com}
Sent: Friday, July 29, 2005 9:13 PM

Dear Members
I am trying to accumulate literature sources that explain how best
to prepare insect specimens for sectioning so that the medium adheres well
and such that the knife doesn't 'catch' the specimen and 'drag' it out of
the medium. I would like to start with semi-thin sections of wings, and
then ultra thin for TEM. If there are any 'inside' tricjs that are
unpublished or otherwise unlikely to be encountered, I would be grateful.
Thanks
Ian

Ian Stocks
308 Long Hall
Clemson University
864 656 5058
istocks-at-clemson.edu


==============================Original Headers==============================
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From: Paul.Perkes-at-asu.edu
Date: Mon, 1 Aug 2005 14:59:05 -0500
Subject: [Microscopy] TEM - Post-Doctoral Research Associate Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Post-Doctoral Research Associate

A post-doctoral research associate position is available in the Center
for Solid State Science at Arizona State University. The research
project involves in situ synthesis and characterization of carbon
nanotubes using a state of the art Environmental Transmission Electron
Microscope, Tecnai F20-ETEM/STEM. Applicants must have a Ph.D in
Chemistry, Physics or Materials Science with experience in
high-resolution imaging, electron energy-loss spectroscopy using
transmission electron microscope. Experience in the areas of scanning
transmission electron microscopy, structural and/or theoretical modeling
is desired. The successful candidate will have an advantage of working
with the cutting edge technology and in a highly motivating environment.

The position is for one year with a start date in August. Deadline is
August 15, 2005, if not filled, weekly thereafter until search closed.
Salary is $34,000/year. Interested candidates must send their resume,
list of publications and the name, address, and phone number of three
references to: Dr Renu Sharma, Center for Solid State Science, Arizona
State University, Tempe, AZ 85287-1704. Email renu.sharma-at-asu.edu.


==============================Original Headers==============================
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From: Eric.Hines-at-csiro.au
Date: Mon, 1 Aug 2005 18:56:56 -0500
Subject: [Microscopy] RE: LM- embedding insect cuticle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ian,
For LM use London Resin and try to match the hardness of the resin to
the tissue eg use the hardest grade for highly sclerotised beetle
cuticle and the medium grade for most cuticles. Tricks like orienting
the block so the knife cuts the cuticle first then cuts through the
underlying tissue will help but the hydrophobic epicuticle of some
insects will never bond to the resin when using standard
glutaraldehyde/osmium fixations. Yell if you want more detail.
Cheers,
Eric Hines
CSIRO Entomology
Canberra OZ

} -----Original Message-----
} From: istocks-at-CLEMSON.EDU [mailto:istocks-at-CLEMSON.EDU]
} Sent: Monday, 1 August 2005 11:26 PM
} To: Hines, Eric (Entomology, Black Mountain)
} Subject: [Microscopy] LM- embedding insect cuticle
}
}
}
}
}
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}
} Dear Members
} I am trying to accumulate literature sources that
} explain how best
} to prepare insect specimens for sectioning so that the medium
} adheres well
} and such that the knife doesn't 'catch' the specimen and
} 'drag' it out of
} the medium. I would like to start with semi-thin sections of
} wings, and
} then ultra thin for TEM. If there are any 'inside' tricjs that are
} unpublished or otherwise unlikely to be encountered, I would
} be grateful. Thanks Ian
}
} Ian Stocks
} 308 Long Hall
} Clemson University
} 864 656 5058
} istocks-at-clemson.edu
}
}
} ==============================Original
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From: hoffpajo-at-yahoo.com
Date: Tue, 2 Aug 2005 10:27:11 -0500
Subject: [Microscopy] snowflakes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

.

--- cammer-at-aecom.yu.edu wrote:

}
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}
} more "amateur" snowflake pics, 6 web pages beginning
} at
} http://cammer.net/blog/snowflake.htm and ending with
} a movie at
} http://cammer.net/blog/snowflake06.htm
}
} Also a favorite at
}
http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm
}
}
}
}
}
} At 08:49 AM 07/29/05 -0500, you wrote:
}
}
}
}
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} }
} } And be sure to check out Wilson A. Bentley's life &
} work. He pioneered
} } snowflake photography a long time ago, and did it
} the hard way. Amateurs
} } rock!
} }
} } Paul Grover
} }
}
} ------------------------------------------------------------------------
} } No matter how much cats fight, there always seem to
} be plenty of kittens.
} } -
} A. Lincoln
} }
} }
} }
} } ==============================Original
} Headers==============================
} } 5, 23 -- From pgrover-at-bilbo.bio.purdue.edu Fri Jul
} 29 08:48:35 2005
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} {pgrover-at-bilbo.bio.purdue.edu}
} } 5, 23 -- To: {microscopy-at-microscopy.com}
} } 5, 23 -- Subject: snowflakes
} } 5, 23 -- Date: Fri, 29 Jul 2005 08:48:35 -0500
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} } ==============================End of -
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}
}
____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility
} Albert Einstein Coll. of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park
} Ave. Bronx, NY 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
} **This electronic transmission contains
} information that is privileged.**
}
}
} ==============================Original
} Headers==============================
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} 11:32:11 2005
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} 12, 23 -- Subject: Re: [Microscopy] snowflakes
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__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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==============================Original Headers==============================
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From: cammer-at-aecom.yu.edu
Date: Tue, 2 Aug 2005 11:05:59 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

First the flame: why assume we only like looking at little itty bitty
things?

On to real comments: Actually, I like looking at anything (size doesn't
count) and I like making cool and/or pretty pictures and I like math puzzles.

Being a microscopist fulfills all of these and pays my bills. To support
my job and my interests, to have access to the cool materials and
technologies that keep me fascinated, I need to be concerned with the
latest multi-probe cutting edge technologies, running a multi-user
facility, having a
purchasing department, federal grants, personnel and a job. Such is reality.

The conclusion is:
We should be discussing both the microscopies (cool pictures and how to
make them regardless whether they are snowflakes for the elementary
classroom or biological processes to understand cutting edge protein
interactions) and the processes for supporting the microscopies (e.g.
federal grants vs. commercial).

I've been subscribed here for years. It seems to me this listserv is doing
exactly what I describe/prescribe. Do we really need to discuss this more?

-Michael

} Esteemed Microscopists,
}
} Since most of you apparently are in Hawaii now, I'll waste a little
} bandwidth and a few KB to vent. I'd like to note that:
}
} (1) This is a MICROSCOPY listserver. This apparently means "looking at
} little things".
}
} (2) There is apparently no requirement that one must be using the latest
} multi-probe cutting edge technology, run a multi-user facility, or have a
} purchasing department, federal grant, or even a job. Our common bond is
} that we like to "look at little things".

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**


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9, 23 -- Subject: Re: [Microscopy] raison d' etre
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From: marc.pypaert-at-yale.edu
Date: Tue, 2 Aug 2005 11:09:11 -0500
Subject: [Microscopy] Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Question to the list:

Our local safety inspector is concerned about the appearance
of the fridge where we stock our solution of osmium. As you
can expect the white interior of the fridge has turned grey
with vapors of osmium over the years. Although we are
taking every precautions to avoid leakage of osmium
vapor from its container, this is a problem that I have
observed in every EM lab that I have worked in. Does
someone on the list have a method to store osmium in such
a way as to avoid any escape of vapor? Also, is there
something we could put into fridge that would trap osmium
vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


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From: hoffpajo-at-yahoo.com
Date: Tue, 2 Aug 2005 11:11:28 -0500
Subject: [Microscopy] raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

he was just kidding around you know.

--- cammer-at-aecom.yu.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
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}
} First the flame: why assume we only like looking at
} little itty bitty
} things?
}
} On to real comments: Actually, I like looking at
} anything (size doesn't
} count) and I like making cool and/or pretty pictures
} and I like math puzzles.
}
} Being a microscopist fulfills all of these and pays
} my bills. To support
} my job and my interests, to have access to the cool
} materials and
} technologies that keep me fascinated, I need to be
} concerned with the
} latest multi-probe cutting edge technologies,
} running a multi-user
} facility, having a
} purchasing department, federal grants, personnel and
} a job. Such is reality.
}
} The conclusion is:
} We should be discussing both the microscopies (cool
} pictures and how to
} make them regardless whether they are snowflakes for
} the elementary
} classroom or biological processes to understand
} cutting edge protein
} interactions) and the processes for supporting the
} microscopies (e.g.
} federal grants vs. commercial).
}
} I've been subscribed here for years. It seems to me
} this listserv is doing
} exactly what I describe/prescribe. Do we really
} need to discuss this more?
}
} -Michael
}
} } Esteemed Microscopists,
} }
} } Since most of you apparently are in Hawaii now,
} I'll waste a little
} } bandwidth and a few KB to vent. I'd like to note
} that:
} }
} } (1) This is a MICROSCOPY listserver. This
} apparently means "looking at
} } little things".
} }
} } (2) There is apparently no requirement that one
} must be using the latest
} } multi-probe cutting edge technology, run a
} multi-user facility, or have a
} } purchasing department, federal grant, or even a
} job. Our common bond is
} } that we like to "look at little things".
}
}
____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility
} Albert Einstein Coll. of Med.
} Jack & Pearl Resnick Campus 1300 Morris Park
} Ave. Bronx, NY 10461
} (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
} **This electronic transmission contains
} information that is privileged.**
}
}
} ==============================Original
} Headers==============================
} 9, 23 -- From cammer-at-aecom.yu.edu Tue Aug 2
} 11:05:59 2005
} 9, 23 -- Received: from mailgw.aecom.yu.edu
} (mailgw.aecom.yu.edu [129.98.1.16])
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} 9, 23 -- To: microscopy-at-microscopy.com
} 9, 23 -- From: Michael Cammer {cammer-at-aecom.yu.edu}
} 9, 23 -- Subject: Re: [Microscopy] raison d' etre
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5, 19 -- Subject: Re: [Microscopy] Re: raison d' etre
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From: hoffpajo-at-yahoo.com
Date: Tue, 2 Aug 2005 11:13:12 -0500
Subject: [Microscopy] Re: Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

you could try placing the bottle in a bath of oil, but
that would get messy.

--- marc.pypaert-at-yale.edu wrote:

}
}
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}
} Question to the list:
}
} Our local safety inspector is concerned about the
} appearance
} of the fridge where we stock our solution of osmium.
} As you
} can expect the white interior of the fridge has
} turned grey
} with vapors of osmium over the years. Although we
} are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I
} have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in
} such
} a way as to avoid any escape of vapor? Also, is
} there
} something we could put into fridge that would trap
} osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original
} Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} 11:09:11 2005
} 5, 18 -- Received: from BIOMED.MED.YALE.EDU
} (biomed.med.yale.edu [130.132.232.48])
} 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id j72G9BnV011512
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} Aug 2005 11:09:11 -0500
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} (net234-111.med.yale.edu [130.132.234.111])
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} 5, 18 -- microscopy-at-microscopy.com; Tue, 02 Aug
} 2005 12:09:29 -0400 (EDT)
} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
} 5, 18 -- In-reply-to:
} {200508021530.j72FU83w031285-at-ns.microscopy.com}
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==============================Original Headers==============================
5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:13:12 2005
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] Osmium vapors
5, 19 -- To: microscopy-at-microscopy.com
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From: hoffpajo-at-yahoo.com
Date: Tue, 2 Aug 2005 11:14:07 -0500
Subject: [Microscopy] Re: raison d' etre

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i was trying to send that directly back to poster. my
bad i guess.

--- hoffpajo-at-yahoo.com wrote:

}
}
}
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} he was just kidding around you know.
}
} --- cammer-at-aecom.yu.edu wrote:
}
} }
} }
} }
} }
}
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} }
} } First the flame: why assume we only like looking
} at
} } little itty bitty
} } things?
} }
} } On to real comments: Actually, I like looking at
} } anything (size doesn't
} } count) and I like making cool and/or pretty
} pictures
} } and I like math puzzles.
} }
} } Being a microscopist fulfills all of these and
} pays
} } my bills. To support
} } my job and my interests, to have access to the
} cool
} } materials and
} } technologies that keep me fascinated, I need to be
} } concerned with the
} } latest multi-probe cutting edge technologies,
} } running a multi-user
} } facility, having a
} } purchasing department, federal grants, personnel
} and
} } a job. Such is reality.
} }
} } The conclusion is:
} } We should be discussing both the microscopies
} (cool
} } pictures and how to
} } make them regardless whether they are snowflakes
} for
} } the elementary
} } classroom or biological processes to understand
} } cutting edge protein
} } interactions) and the processes for supporting the
} } microscopies (e.g.
} } federal grants vs. commercial).
} }
} } I've been subscribed here for years. It seems to
} me
} } this listserv is doing
} } exactly what I describe/prescribe. Do we really
} } need to discuss this more?
} }
} } -Michael
} }
} } } Esteemed Microscopists,
} } }
} } } Since most of you apparently are in Hawaii now,
} } I'll waste a little
} } } bandwidth and a few KB to vent. I'd like to note
} } that:
} } }
} } } (1) This is a MICROSCOPY listserver. This
} } apparently means "looking at
} } } little things".
} } }
} } } (2) There is apparently no requirement that one
} } must be using the latest
} } } multi-probe cutting edge technology, run a
} } multi-user facility, or have a
} } } purchasing department, federal grant, or even a
} } job. Our common bond is
} } } that we like to "look at little things".
} }
} }
}
____________________________________________________________________________
} } Michael Cammer Analytical Imaging Facility
} } Albert Einstein Coll. of Med.
} } Jack & Pearl Resnick Campus 1300 Morris Park
} } Ave. Bronx, NY 10461
} } (718) 430-2890 Fax: 430-8996 URL:
} } http://www.aecom.yu.edu/aif/
} } **This electronic transmission contains
} } information that is privileged.**
} }
} }
} } ==============================Original
} } Headers==============================
} } 9, 23 -- From cammer-at-aecom.yu.edu Tue Aug 2
} } 11:05:59 2005
} } 9, 23 -- Received: from mailgw.aecom.yu.edu
} } (mailgw.aecom.yu.edu [129.98.1.16])
} } 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8)
} with
} } ESMTP id j72G5wXq005120
} } 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2
} } Aug 2005 11:05:59 -0500
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} } (mailvx.aecom.yu.edu [129.98.1.17])
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} } with SMTP id j72G5Jb5023564
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} } Aug 2005 12:05:58 -0400
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} } ([129.98.1.100])
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} 3.1.1.32)
} } with SMTP id M2005080212055813185
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} } Aug 2005 12:05:58 -0400
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} ESMTP
} } id 91E0B2FC6
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} } Aug 2005 12:05:58 -0400 (EDT)
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} }
}
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} } 5.2.1
} } 9, 23 -- Date: Tue, 02 Aug 2005 12:05:58 -0400
} } 9, 23 -- To: microscopy-at-microscopy.com
} } 9, 23 -- From: Michael Cammer
} {cammer-at-aecom.yu.edu}
} } 9, 23 -- Subject: Re: [Microscopy] raison d' etre
} } 9, 23 -- In-Reply-To:
} } {200507291437.j6TEboiR011778-at-ns.microscopy.com}
} } 9, 23 -- Mime-Version: 1.0
} } 9, 23 -- Content-Type: text/plain;
} } charset="us-ascii"; format=flowed
} } ==============================End of -
} } Headers==============================
} }
}
}
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} protection around
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} ==============================Original
} Headers==============================
} 5, 19 -- From hoffpajo-at-yahoo.com Tue Aug 2 11:11:28
} 2005
} 5, 19 -- Received: from web50204.mail.yahoo.com
} (web50204.mail.yahoo.com [206.190.38.45])
} 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with



From: msimms-at-tracelabs.com
Date: Tue, 2 Aug 2005 11:19:49 -0500
Subject: [Microscopy] Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Tuesday, August 02, 2005 11:13 AM
To: msimms-at-tracelabs.com

Question to the list:

Our local safety inspector is concerned about the appearance
of the fridge where we stock our solution of osmium. As you
can expect the white interior of the fridge has turned grey
with vapors of osmium over the years. Although we are
taking every precautions to avoid leakage of osmium
vapor from its container, this is a problem that I have
observed in every EM lab that I have worked in. Does
someone on the list have a method to store osmium in such
a way as to avoid any escape of vapor? Also, is there
something we could put into fridge that would trap osmium
vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original Headers==============================
5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu
[130.132.232.48])
5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id
j72G9BnV011512
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-0500
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5, 18 -- microscopy-at-microscopy.com; Tue, 02 Aug 2005 12:09:29 -0400 (EDT)
5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
5, 18 -- Subject: Osmium vapors
5, 18 -- In-reply-to: {200508021530.j72FU83w031285-at-ns.microscopy.com}
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==============================Original Headers==============================
16, 15 -- From msimms-at-tracelabs.com Tue Aug 2 11:19:49 2005
16, 15 -- Received: from mail0.methode.com (mail0.methode.com [12.182.227.149])
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16, 15 -- From: "Simms, Michael" {msimms-at-tracelabs.com}
16, 15 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
16, 15 -- Subject: RE: [Microscopy] Osmium vapors
16, 15 -- Date: Tue, 2 Aug 2005 11:19:33 -0500
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From: dmclea-at-sandia.gov
Date: Tue, 2 Aug 2005 11:23:07 -0500
Subject: [Microscopy] Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Marc

We store our osmium in a secondary container...a large poly pro bottle
(capped) and then we bag that container in a plastic Ziploc. It helps a
bit.

Dorrance

-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Tuesday, August 02, 2005 9:10 AM
To: McLean, Dorrance

Question to the list:

Our local safety inspector is concerned about the appearance of the
fridge where we stock our solution of osmium. As you can expect the
white interior of the fridge has turned grey with vapors of osmium over
the years. Although we are taking every precautions to avoid leakage of
osmium vapor from its container, this is a problem that I have observed
in every EM lab that I have worked in. Does someone on the list have a
method to store osmium in such a way as to avoid any escape of vapor?
Also, is there something we could put into fridge that would trap osmium
vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


==============================Original
Headers==============================
5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005 5, 18 --
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5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5, 18 -- From: Marc
Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject: Osmium vapors 5, 18 --
In-reply-to: {200508021530.j72FU83w031285-at-ns.microscopy.com}
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16, 29 -- From dmclea-at-sandia.gov Tue Aug 2 11:23:07 2005
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From: tiekotte-at-up.edu
Date: Tue, 2 Aug 2005 11:26:35 -0500
Subject: [Microscopy] Re: Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When OsO4 came in metal cans, I found by placing the working solution in a
Wheaton dropper bottle, wrap the bulb with Parafilm, place the bottle in the
can, close the lid, wrap the lid with electrician tape, and place the can in
a plastic Ziplock bag worked great. You could essentially use any clean
glass container, not just a Wheaton bottle.

After 20 years of working with OsO4, the inside of the refrigerator was
pristine. It requires diligence to unwrap and wrap the container, but it
worked.

A short-cut to the above mentioned technique, was to use a clean pint paint,
place the Wheaton bottle in the can and push the lid down tight, then place
the entire can in a Ziplock bag.

Regards,
Ken
_______________________________________
Kenneth L. Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N Willamette Blvd.
Portland, OR 97203 USA

Tel.: 503.943.8861
Email: tiekotte-at-up.edu


On 8/2/05 9:09 AM, "marc.pypaert-at-yale.edu" {marc.pypaert-at-yale.edu} wrote:

}
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}
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Question to the list:
}
} Our local safety inspector is concerned about the appearance
} of the fridge where we stock our solution of osmium. As you
} can expect the white interior of the fridge has turned grey
} with vapors of osmium over the years. Although we are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in such
} a way as to avoid any escape of vapor? Also, is there
} something we could put into fridge that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
} 5, 18 -- Received: from BIOMED.MED.YALE.EDU (biomed.med.yale.edu
} [130.132.232.48])
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
} 5, 18 -- In-reply-to: {200508021530.j72FU83w031285-at-ns.microscopy.com}
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==============================Original Headers==============================
9, 17 -- From tiekotte-at-up.edu Tue Aug 2 11:26:35 2005
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9, 17 -- Subject: Re: [Microscopy] Osmium vapors
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From: hoffpajo-at-yahoo.com
Date: Tue, 2 Aug 2005 11:35:26 -0500
Subject: [Microscopy] Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

in the alternative and it may be more expensive and
you may not want to go there. and if someone with a
better memory than i do anymore, can correct me. i do
remember seeing premade OsO4 in small amounts. use
what you need, perhaps even batch the tissue samples.
then throw out the unused in the waste.
just a thought.

--- dmclea-at-sandia.gov wrote:

}
}
}
}
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}
} Marc
}
} We store our osmium in a secondary container...a
} large poly pro bottle
} (capped) and then we bag that container in a plastic
} Ziploc. It helps a
} bit.
}
} Dorrance
}
} -----Original Message-----
} X-from: marc.pypaert-at-yale.edu
} [mailto:marc.pypaert-at-yale.edu]
} Sent: Tuesday, August 02, 2005 9:10 AM
} To: McLean, Dorrance
} Subject: [Microscopy] Osmium vapors
}
}
}
}
}
------------------------------------------------------------------------
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} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of
} America To Subscribe/Unsubscribe --
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} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
} ----
}
} Question to the list:
}
} Our local safety inspector is concerned about the
} appearance of the
} fridge where we stock our solution of osmium. As you
} can expect the
} white interior of the fridge has turned grey with
} vapors of osmium over
} the years. Although we are taking every precautions
} to avoid leakage of
} osmium vapor from its container, this is a problem
} that I have observed
} in every EM lab that I have worked in. Does someone
} on the list have a
} method to store osmium in such a way as to avoid any
} escape of vapor?
} Also, is there something we could put into fridge
} that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original
} Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} 11:09:11 2005 5, 18 --
} Received: from BIOMED.MED.YALE.EDU
} (biomed.med.yale.edu
} [130.132.232.48])
} 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id
} j72G9BnV011512
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} Aug 2005
} 11:09:11 -0500
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} 18 -- microscopy-at-microscopy.com; Tue, 02 Aug 2005
} 12:09:29 -0400 (EDT)
} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5, 18
} -- From: Marc
} Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject:
} Osmium vapors 5, 18 --
} In-reply-to:
} {200508021530.j72FU83w031285-at-ns.microscopy.com}
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} Headers==============================
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} 2005
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____________________________________________________
Start your day with Yahoo! - make it your home page
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From: gunkelrl-at-slu.edu
Date: Tue, 2 Aug 2005 11:47:48 -0500
Subject: [Microscopy] Re: Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We use Osmium in our lab and keep it in the refrigerator.
We keep it in a glass bottle and cover the lid with
parafilm. Then we put that bottle in another bottle with a
screw top and wrap the bottle in foil. It works great, our
refrigerator doesn't have and black residue, just the two
bottles.

rebecca

==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Tue, 2 Aug 2005 11:49:26 -0500
Subject: [Microscopy] Re: Mannie Steglich

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


is mannie Mannie Steglich still around? haven't heard
from him in a while.
john

--- hoffpajo-at-yahoo.com wrote:

}
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}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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}
} in the alternative and it may be more expensive and
} you may not want to go there. and if someone with a
} better memory than i do anymore, can correct me. i
} do
} remember seeing premade OsO4 in small amounts. use
} what you need, perhaps even batch the tissue
} samples.
} then throw out the unused in the waste.
} just a thought.
}
} --- dmclea-at-sandia.gov wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------------
} }
} } Marc
} }
} } We store our osmium in a secondary container...a
} } large poly pro bottle
} } (capped) and then we bag that container in a
} plastic
} } Ziploc. It helps a
} } bit.
} }
} } Dorrance
} }
} } -----Original Message-----
} } X-from: marc.pypaert-at-yale.edu
} } [mailto:marc.pypaert-at-yale.edu]
} } Sent: Tuesday, August 02, 2005 9:10 AM
} } To: McLean, Dorrance
} } Subject: [Microscopy] Osmium vapors
} }
} }
} }
} }
} }
}
------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
------------------------------------------------------------------------
} } ----
} }
} } Question to the list:
} }
} } Our local safety inspector is concerned about the
} } appearance of the
} } fridge where we stock our solution of osmium. As
} you
} } can expect the
} } white interior of the fridge has turned grey with
} } vapors of osmium over
} } the years. Although we are taking every
} precautions
} } to avoid leakage of
} } osmium vapor from its container, this is a problem
} } that I have observed
} } in every EM lab that I have worked in. Does
} someone
} } on the list have a
} } method to store osmium in such a way as to avoid
} any
} } escape of vapor?
} } Also, is there something we could put into fridge
} } that would trap osmium
} } vapors as they leak out? Thanks for your advise.
} }
} } Marc
} }
} } --
} } Marc Pypaert
} } Department of Cell Biology
} } Center for Cell and Molecular Imaging
} } Ludwig Institute for Cancer Research
} } Yale University School of Medicine
} } 333 Cedar Street, PO Box 208002
} } New Haven, CT 06520-8002
} } TEL 203-785 3681
} } FAX 203-785 7446
} }
} }
} } ==============================Original
} } Headers==============================
} } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } 11:09:11 2005 5, 18 --
} } Received: from BIOMED.MED.YALE.EDU
} } (biomed.med.yale.edu
} } [130.132.232.48])
} } 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8)
} with
} } ESMTP id
} } j72G9BnV011512
} } 5, 18 -- for {microscopy-at-microscopy.com} ; Tue, 2
} } Aug 2005
} } 11:09:11 -0500
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} } (net234-111.med.yale.edu
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} biomed.med.yale.edu
} } (PMDF V6.1-1 #30532)
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} } {01LRCOV1R88000DGI6-at-biomed.med.yale.edu} for 5,
} } 18 -- microscopy-at-microscopy.com; Tue, 02 Aug 2005
} } 12:09:29 -0400 (EDT)
} } 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5,
} 18
} } -- From: Marc
} } Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject:
} } Osmium vapors 5, 18 --
} } In-reply-to:
} } {200508021530.j72FU83w031285-at-ns.microscopy.com}
} } 5, 18 -- To: microscopy-at-microscopy.com
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} framework
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From: jfactor-at-ns.purchase.edu
Date: Tue, 2 Aug 2005 11:51:40 -0500
Subject: [Microscopy] Re: Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Working with undergraduates in a small (yet multiuser) lab, I have had
concerns about handling and storing osmium tetroxide for years. My
solution (which may not be practical for everyone) is that only sealed
ampoules are stored in the fridge (in their original shipping can and
packing materials). Vials are opened one at a time, the solutions are
mixed and used, tissues are exposed, left-over supplies are stored, and
waste is kept ONLY in the fume hood. In other words, opened supplies of
osmium never leave the fume hood (until disposal as toxic waste). If a
cold solution is needed, and ice bath can be used. We mix just about
enough for each procedure, and the unused left-over Os04 working
solution is kept in a small glass jar in the hood for the next
procedure. So we have no blackened refrigerator surfaces and no Os04
fumes accumulating in the fridge (or escaping into the room). It seems
to me that this approach could be expanded for a larger lab.

--Jan

---------------------------------------8/2/05
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College
State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------


marc.pypaert-at-yale.edu wrote:8/2/05
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} Question to the list:
}
} Our local safety inspector is concerned about the appearance
} of the fridge where we stock our solution of osmium. As you
} can expect the white interior of the fridge has turned grey
} with vapors of osmium over the years. Although we are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in such
} a way as to avoid any escape of vapor? Also, is there
} something we could put into fridge that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original Headers==============================
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} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
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From: msteglic-at-mdanderson.org
Date: Tue, 2 Aug 2005 12:00:04 -0500
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Still alive and kikking in Houston.





hoffpajo-at-yahoo.com

08/02/2005 11:52 AM
Please respond to microscopy




To:
msteglic-at-mdanderson.org
cc:







is mannie Mannie Steglich still around? haven't heard
from him in a while.
john

--- hoffpajo-at-yahoo.com wrote:

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}
} in the alternative and it may be more expensive and
} you may not want to go there. and if someone with a
} better memory than i do anymore, can correct me. i
} do
} remember seeing premade OsO4 in small amounts. use
} what you need, perhaps even batch the tissue
} samples.
} then throw out the unused in the waste.
} just a thought.
}
} --- dmclea-at-sandia.gov wrote:
}
} }
} }
} }
} }
}
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} }
} } Marc
} }
} } We store our osmium in a secondary container...a
} } large poly pro bottle
} } (capped) and then we bag that container in a
} plastic
} } Ziploc. It helps a
} } bit.
} }
} } Dorrance
} }
} } -----Original Message-----
} } X-from: marc.pypaert-at-yale.edu
} } [mailto:marc.pypaert-at-yale.edu]
} } Sent: Tuesday, August 02, 2005 9:10 AM
} } To: McLean, Dorrance
} } Subject: [Microscopy] Osmium vapors
} }
} }
} }
} }
} }
}
------------------------------------------------------------------------
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} } America To Subscribe/Unsubscribe --
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} } ----
} }
} } Question to the list:
} }
} } Our local safety inspector is concerned about the
} } appearance of the
} } fridge where we stock our solution of osmium. As
} you
} } can expect the
} } white interior of the fridge has turned grey with
} } vapors of osmium over
} } the years. Although we are taking every
} precautions
} } to avoid leakage of
} } osmium vapor from its container, this is a problem
} } that I have observed
} } in every EM lab that I have worked in. Does
} someone
} } on the list have a
} } method to store osmium in such a way as to avoid
} any
} } escape of vapor?
} } Also, is there something we could put into fridge
} } that would trap osmium
} } vapors as they leak out? Thanks for your advise.
} }
} } Marc
} }
} } --
} } Marc Pypaert
} } Department of Cell Biology
} } Center for Cell and Molecular Imaging
} } Ludwig Institute for Cancer Research
} } Yale University School of Medicine
} } 333 Cedar Street, PO Box 208002
} } New Haven, CT 06520-8002
} } TEL 203-785 3681
} } FAX 203-785 7446
} }
} }
} } ==============================Original
} } Headers==============================
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} } 11:09:11 2005 5, 18 --
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} } 12:09:29 -0400 (EDT)
} } 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400 5,
} 18
} } -- From: Marc
} } Pypaert {marc.pypaert-at-yale.edu} 5, 18 -- Subject:
} } Osmium vapors 5, 18 --
} } In-reply-to:
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} 11:23:07
} } 2005
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From: phillipst-at-missouri.edu
Date: Tue, 2 Aug 2005 12:03:30 -0500
Subject: [Microscopy] Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The simplest solution to this problem is not to keep your osmium in the
refrigerator. I keep mine in a glass Schott bottle with plastic cap which
is then stored within a plastic container in the fume hood. I am not sure
if light effects the osmium but to be sure, I wrap the plastic container in
aluminum foil. There is leakage from the glass bottle because over time,
the inside of the plastic container goes black. Anything that leaks past
the secondary container goes up the fume hood. Once every year or two, I
splurge and replace the bottle and container. I don't experience
"pre-mature" darkening of my 2% osmium stock which typically lasts about
2-3 months before I use it all up. Tom Phillips



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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7, 18 -- To: Microscopy-at-msa.microscopy.com
7, 18 -- From: Tom Phillips {phillipst-at-missouri.edu}
7, 18 -- Subject: Osmium vapors
7, 18 -- Mime-Version: 1.0
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==============================End of - Headers==============================




From: hoffpajo-at-yahoo.com
Date: Tue, 2 Aug 2005 12:06:27 -0500
Subject: [Microscopy] Re: Mannie Steglich

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

well thats good to know. is it cooler there than here?

--- msteglic-at-mdanderson.org wrote:

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} Still alive and kikking in Houston.
}
}
}
}
}
} hoffpajo-at-yahoo.com
}
} 08/02/2005 11:52 AM
} Please respond to microscopy
}
}
}
}
} To:
} msteglic-at-mdanderson.org
} cc:
}
}
}
}
}
} Subject:
} [Microscopy] Re: Mannie Steglich
}
}
}
}
}
}
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}
} is mannie Mannie Steglich still around? haven't
} heard
} from him in a while.
} john
}
} --- hoffpajo-at-yahoo.com wrote:
}
} }
} }
} }
} }
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} }
} } in the alternative and it may be more expensive
} and
} } you may not want to go there. and if someone with
} a
} } better memory than i do anymore, can correct me. i
} } do
} } remember seeing premade OsO4 in small amounts. use
} } what you need, perhaps even batch the tissue
} } samples.
} } then throw out the unused in the waste.
} } just a thought.
} }
} } --- dmclea-at-sandia.gov wrote:
} }
} } }
} } }
} } }
} } }
} }
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} } }
} } } Marc
} } }
} } } We store our osmium in a secondary container...a
} } } large poly pro bottle
} } } (capped) and then we bag that container in a
} } plastic
} } } Ziploc. It helps a
} } } bit.
} } }
} } } Dorrance
} } }
} } } -----Original Message-----
} } } X-from: marc.pypaert-at-yale.edu
} } } [mailto:marc.pypaert-at-yale.edu]
} } } Sent: Tuesday, August 02, 2005 9:10 AM
} } } To: McLean, Dorrance
} } } Subject: [Microscopy] Osmium vapors
} } }
} } }
} } }
} } }
} } }
} }
}
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} } } The Microscopy ListServer -- CoSponsor: The
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} } } ----
} } }
} } } Question to the list:
} } }
} } } Our local safety inspector is concerned about
} the
} } } appearance of the
} } } fridge where we stock our solution of osmium. As
} } you
} } } can expect the
} } } white interior of the fridge has turned grey
} with
} } } vapors of osmium over
} } } the years. Although we are taking every
} } precautions
} } } to avoid leakage of
} } } osmium vapor from its container, this is a
} problem
} } } that I have observed
} } } in every EM lab that I have worked in. Does
} } someone
} } } on the list have a
} } } method to store osmium in such a way as to avoid
} } any
} } } escape of vapor?
} } } Also, is there something we could put into
} fridge
} } } that would trap osmium
} } } vapors as they leak out? Thanks for your advise.
} } }
} } } Marc
} } }
} } } --
} } } Marc Pypaert
} } } Department of Cell Biology
} } } Center for Cell and Molecular Imaging
} } } Ludwig Institute for Cancer Research
} } } Yale University School of Medicine
} } } 333 Cedar Street, PO Box 208002
} } } New Haven, CT 06520-8002
} } } TEL 203-785 3681
} } } FAX 203-785 7446
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } } 11:09:11 2005 5, 18 --
} } } Received: from BIOMED.MED.YALE.EDU
} } } (biomed.med.yale.edu
} } } [130.132.232.48])
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} (8.12.11/8.12.8)
} } with
} } } ESMTP id
} } } j72G9BnV011512
} } } 5, 18 -- for
} {microscopy-at-microscopy.com} ; Tue, 2
} } } Aug 2005
}
=== message truncated ===


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From: microbill-at-mohawk.net
Date: Tue, 2 Aug 2005 12:47:36 -0500
Subject: [Microscopy] Mannie Steglich

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The last I knew he was at MD Anderson in Texas - but he may well have
retired by now - see http://woodenwonderstx.com/About.html




At 12:49 PM 8/2/2005, you wrote:



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From: tttan-at-simtech.a-star.edu.sg
Date: Tue, 2 Aug 2005 12:59:37 -0500
Subject: [Microscopy] viaWWW: Field Ion Microscope

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tttan-at-simtech.a-star.edu.sg) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
August 1, 2005 at 20:24:16
---------------------------------------------------------------------------

Email: tttan-at-simtech.a-star.edu.sg
Name: TT Tan

Organization: Singapore Inst. of Manuf. Tech.

Title-Subject: [Filtered] Field Ion Microscope

Question: Hi all,

I would like to know from who can I buy the glassware to do field ion
microscopy.

I am looking for a quartz ware that can do the job. Preferably one
that allows me to fit onto a NW40 joint.

Thanks in advance.

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: winston.wiggins-at-cshs.org
Date: Tue, 2 Aug 2005 13:00:05 -0500
Subject: [Microscopy] viaWWW: Osmium Vapors

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (winston.wiggins-at-cshs.org) from
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on
Tuesday, August 2, 2005 at 12:15:04
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Email: winston.wiggins-at-cshs.org
Name: Winston Wiggins

Organization: Cedars-Sinai HealthCare System

Title-Subject: [Filtered] MListserver: Osmium Vapors

Question: I keep my working solution of Osmium in a glass Wheaton or
Gibco bottle with a Teflon-lined cap and wrap the cap with Parafilm
and put that into the metal can that's shipped with the ampoules of
Osmium or a larger glass jar. I layer molecular sieves in the
can/jar. Any escaping Osmium vapors are indicated by blackening
Parafilm... or blackening refrigerator!
Our frig is clean.

Plastic is permeable to Osmium - use glass!

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From: sswaffe-at-abv.bg
Date: Tue, 2 Aug 2005 13:27:41 -0500
Subject: [Microscopy] AskAMicroscopist: numerical aperture

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sswaffe-at-abv.bg) from
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on Tuesday, August 2, 2005 at 12:24:49
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: National High School of Mathematics and Science

Education: 9-12th Grade High School

Location: Sofia, Bulgaria

Question: What exactly is the N.A. or numerical aperture and why on
my condensor it's value is 0.9 and on my high power objetive it is
1.25?

---------------------------------------------------------------------------

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From: frank.karl-at-degussa.com
Date: Tue, 2 Aug 2005 13:45:23 -0500
Subject: [Microscopy] Re: AskAMicroscopist: numerical aperture

Contents Retrieved from Microscopy Listserver Archives
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Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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From: frank.karl-at-degussa.com
Date: Tue, 2 Aug 2005 14:11:25 -0500
Subject: [Microscopy] Re: AskAMicroscopist: numerical aperture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

NA is 0.5 of AA. Or more correctly NA = n(sine of (AA/2)). The notation n
is the refractive index between your lens and the glass slide or cover
slip, usually air n=1.0000. This isn't very helpful is it? AA or angular
aperture is a measure of the angle which describes the largest come of
light your objective will accept or your condenser will produce.
Resolution depends (simple theory) capturing as many of the defracted rays
from a sample as possible. The bigger the cone the more rays you capture
and the more resolution you have and the more you can magnify the sample.

Optics meant to be used in air always have an NA less than 1. Those using
oil, glycine or water have a NA greater than one. Condensers typically
have a higher NA than your assortment of objectives because they have to
accommodate many objectives. You reduce the NA of a condenser by closing
the condenser iris down. If you have the correct illumination set up you
can remove an eyepiece and watch the condenser iris close in the back focal
plane of the objective. Most of us close it a little past the edge of the
objective back focal plane 'cause we like it contrasty.


So your condenser is an meant to use in air while your objective is an oil
emersion. Never, never, say it with me, never put beans in your nose or
oil on a condenser with an NA of 0.9.

The best resolution you can get with your system is to oil the objective to
the slide and use the condenser in air. Frankly, it's just OK that way and
in my opinion it's oil immersion is never worth the work (OK you can flame
me now...) You need to oil both the condenser and objective to the slide
(assuming you have the right thickness slide, cover slip and your sample
has sufficient contrast by staining or difference in refractive index) to
get the most out of your optical system. I'm a microscopist cause I'm a
big sloppy guy and this is all too much trouble for me.

Sound like a homework question, so good luck!!!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
messages attached to it may contain information that is confidential or
legally privileged. If you are not the intended recipient, or a person
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in or attached to this transmission is STRICTLY PROHIBITED. If you have
received this transmission in error, please immediately notify the sender
by telephone or return e-mail and delete the original transmission and its
attachments without reading or saving in any manner. Thank you.



sswaffe-at-abv.bg
To: frank.karl-at-degussa.com
08/02/2005 02:28 cc:
PM Subject: [Microscopy] AskAMicroscopist: numerical aperture
Please respond to
microscopy








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Below is the result of your feedback form (NJZFM-ultra-55). It was
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http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, August 2, 2005 at 12:24:49
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: National High School of Mathematics and Science

Education: 9-12th Grade High School

Location: Sofia, Bulgaria

Question: What exactly is the N.A. or numerical aperture and why on
my condensor it's value is 0.9 and on my high power objetive it is
1.25?

---------------------------------------------------------------------------

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From: gcc-at-couger.com
Date: Tue, 2 Aug 2005 14:36:45 -0500
Subject: [Microscopy] Re: AskAMicroscopist: numerical aperture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Sophia,

In air a na of .9 is all that is possible with out oiling the
condenser to the slide. Condensers made with .9 na make better
images with out oil than 1.2 na to 1.4 na oil immersion
condensers with out oil. Also simple Abby condensers have a good
deal of chromic aberration at wide apertures.

The combination of those and the fact that most users don't take
the time to oil the condenser to the bottom of the slide
resulted in many microscope makers using .9 na condensers. For a
in depth discussion of the problems of large na condenser and
other things see Ted Clark's piece
http://www.modernmicroscopy.com/main.asp?article=53 in Modern
Microscopy. Other work on lighting by Ted Clarke is referenced
at http://www.couger.com/microscope/Ted-Clarke/

You asked a good questioning about the aperture of the
condenser. To get the full performance form an objective the
condenser and light train need to be the same quality and na as
the objective. That is usually only a real consideration for
study at high resolution and high resolution microphotography.
The limiting factor in any optic system is its weakest link. In
micosopes this often the lighting and/or condeser.

Best Regards
Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org
sswaffe-at-abv.bg wrote:

} Email: sswaffe-at-abv.bg
} Name: Veselin Andreev
}
} Organization: National High School of Mathematics and Science
}
} Education: 9-12th Grade High School
}
} Location: Sofia, Bulgaria
}
} Question: What exactly is the N.A. or numerical aperture and
why on
} my condensor it's value is 0.9 and on my high power objetive
it is
} 1.25?


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From: waltk-at-pptli.com
Date: Tue, 2 Aug 2005 15:26:43 -0500
Subject: [Microscopy] AskAMicroscopist: numerical aperture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The numerical aperture is a rating for how much light comes through. A
variety of design factors that I can't explain affect the value. An
excellent source of information for your class is available on the Olympus
site. I've pasted the address of the page discussing objectives and N.A.
below. From there you can navigate throughout their entire primer.

http://olympusmicro.com/primer/anatomy/objectives.html

I don't have any connection to Olympus and other manufacturers may also have
excellent technical information up on the web. I suggested this site
because I found it to be very well organized, illustrated, and easy to
understand. Aside from the liberal use of the "Olympus" label, the
discussions are based on science, not a sales pitch. I hope this helps.

Walt Klonowski
Pikes Peak Test Labs



-----Original Message-----
X-from: sswaffe-at-abv.bg [mailto:sswaffe-at-abv.bg]
Sent: Tuesday, August 02, 2005 12:31 PM
To: WaltK-at-pptli.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (sswaffe-at-abv.bg) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, August 2, 2005 at 12:24:49
---------------------------------------------------------------------------

Email: sswaffe-at-abv.bg
Name: Veselin Andreev

Organization: National High School of Mathematics and Science

Education: 9-12th Grade High School

Location: Sofia, Bulgaria

Question: What exactly is the N.A. or numerical aperture and why on
my condensor it's value is 0.9 and on my high power objetive it is
1.25?

---------------------------------------------------------------------------

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From: bfoster-at-mme1.com
Date: Tue, 2 Aug 2005 16:01:56 -0500
Subject: [Microscopy] AskAMicroscopist: numerical aperture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Veselin,

The numerical aperture is the light collecting ability of a specific piece of optics. It depends on two factors: half the actual collecting angle and the refractive index of the immersion fluid. The equation is:
NA=n x sin a where n = ri of the immersion fluid (air is 1.00, oil is 1.5212, water is 1.33, for example) and " a" is the half angle.

Your question is an interesting one for several reasons.

First, both the NA of the condenser and the NA of the objective contribute to resolution: (Equations are difficult to send by email, but here is the basic equation:)
R = [1.2 L]/[NAo + NAc] where 1.2 is a shape factor (Bessel function) reflecting the round apertures we use in the microscope, "L" is the wavelength of light (you can use 500nm or 0.500microns as an average), and NAo = NA objective; NAc = NA condenser.

Interestingly, this equation is related to not only the ability to resolve spacing (R is actually the smallest distance between two objects which still permits them to be imaged as 2 separate entities), it also has impact on edge fidelity. As a result, higher NA optics (both objective and condenser) not only improve your ability to resolve fine detail, they also produce crisper, sharper edges.

Secondly, the NA's written on your glassware are only the general operating specs. If you close down the iris in the condenser, you limit the NA of the condenser. Since your condenser has a maximum NA of 0.9,it is not meant to be used with oil. On the other hand, your objective NA indicates that, for best imaging, a drop of the appropriate immersion oil should be placed between it and the top of your sample. If you don't follow this procedure, your images will not only lack clarity (immersion oil is considered to be an important optical component of the system), your objective will only operate with a "working NA" of approximately 0.9.

Finally, your question is very appropriate because, for optimum resolution, the NA of the condenser should match or exceed the NA of your objective. Your condenser does not meet this requirement. If you do the calculations with the Resolution equation above, you will see that you will limit the resolution available from your system. Since you are working at the High School level, this limitation will probably not have a serious impact on your work. If you eventually get involved with higher level research, I would recommend that you purchase a condenser which better fits your needs.

All of this is explained in "Optimizing Light Microscopy," a book still available through MME. For further information, please contact Ken Piel at kenpiel-at-mme1.com. (Tell him I sent you).

Hope this is helpful and good luck in your studies!

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Fall? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.



At 03:30 PM 8/2/2005, waltk-at-pptli.com wrote:



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From: A.W.Hicks-at-massey.ac.nz
Date: Tue, 2 Aug 2005 17:20:17 -0500
Subject: [Microscopy] Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello to you all,

We keep our osmium solutions in Schott Duran laboratory bottles
(http://www.schott-duran.com/english/products/duran/detail/laborflaschen
.html), and keep the bottles in a paint can (a new one that never had
paint in it). This is then kept in our fridge. This arrangement seems to
keep the vapour contained (no marks in our fridge) and isn't a pain to
open up. I also keep a pottle of whole milk powder in there for spills.

Aaron Hicks
Electron Microscopy Preparation Technician

Comparative Physiology and Anatomy
Institute of Veterinary, Animal, and Biomedical Sciences
Massey University

PN-412
Private Bag 11 222
Palmerston North
New Zealand

Phone +64 06 350 4874


-----Original Message-----
X-from: marc.pypaert-at-yale.edu [mailto:marc.pypaert-at-yale.edu]
Sent: Wednesday, 3 August 2005 4:12 a.m.
To: Hicks, Aaron

Question to the list:

Our local safety inspector is concerned about the appearance
of the fridge where we stock our solution of osmium. As you
can expect the white interior of the fridge has turned grey with vapors
of osmium over the years. Although we are taking every precautions to
avoid leakage of osmium vapor from its container, this is a problem that
I have observed in every EM lab that I have worked in. Does someone on
the list have a method to store osmium in such a way as to avoid any
escape of vapor? Also, is there something we could put into fridge that
would trap osmium vapors as they leak out? Thanks for your advise.

Marc

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446


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From: David.Patton-at-uwe.ac.uk
Date: Wed, 3 Aug 2005 06:22:04 -0500
Subject: [Microscopy] Re: Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Osmium vapours are not stopped by simple bottle tops. We buy ours
already made up. The bottle lids have a plastic (?) insert which is
effective.

Waste osmium is stored in any bottle which is then kept in what we can
in the UK a Kilner jar. It is made of glass, has a rubber seal and uses
wire to clamp the lid very tightly. Osmium vapours do not seem to pass
through rubber seals. Unfortunately new Kilner jars in my supermarket
have plastic seals - so an experiment will be required.

Dave

-----Original Message-----
X-from: gunkelrl-at-slu.edu [mailto:gunkelrl-at-slu.edu]
Sent: 02 August 2005 17:49
To: David Patton

We use Osmium in our lab and keep it in the refrigerator.
We keep it in a glass bottle and cover the lid with
parafilm. Then we put that bottle in another bottle with a
screw top and wrap the bottle in foil. It works great, our
refrigerator doesn't have and black residue, just the two
bottles.

rebecca

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From: jose-at-svi.nl
Date: Wed, 3 Aug 2005 08:06:23 -0500
Subject: [Microscopy] Re: AskAMicroscopist: numerical aperture

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Dear Veselin, dear list members,

appart from the good explanations you already received from other list
members, I recommend you another link where you can also find explanations
on how the NA determines the microscope resolution:

http://support.svi.nl/wiki/NumericalAperture

As this is a wiki site, you can also share your knowledge by editing it!

Regards,

jose.

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From: howzaluzec-at-microscopy.com
Date: Wed, 3 Aug 2005 08:31:45 -0500
Subject: [Microscopy] Astounding Refinances in 24 hours.

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Hola

Homeowner

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This offer is being extended to you unconditionally and your credit is in no way a factor.

To take Advantage of this Limited Time opportunity

All we ask is that you visit our Website and complete
The 1 minute post Approval Form

http://dXY6z.fastlow-rates.com/5/index/ryn/TdWLZhvXrx

Good Day,

Rachelle Mayo

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From: jose-at-svi.nl
Date: Wed, 3 Aug 2005 08:38:09 -0500
Subject: [Microscopy] Re: AskAMicroscopist: numerical aperture

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Dear Nestor,

will replies like these reach the sender? I mean, this question was posted
from AskAMicroscopist, not emailed by a list member, so I am afraid our
replies will not reach people like Veselin unless we explicity include
them in the address of our emails...

jose.

==============================Original Headers==============================
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From: SHem-at-laurentian.ca
Date: Wed, 3 Aug 2005 10:05:49 -0500
Subject: [Microscopy] Cambridge S-250

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Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem







_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html



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From: bfoster-at-mme1.com
Date: Wed, 3 Aug 2005 10:26:10 -0500
Subject: [Microscopy] Re: Cambridge S-250

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Hi, Skage

We have a consultant who has a lot of experience in this area and also provides training. I"ll forward your email to him

Best regards
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Fall? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.


At 10:08 AM 8/3/2005, SHem-at-laurentian.ca wrote:



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From: as-at-astonmet.com
Date: Wed, 3 Aug 2005 10:28:13 -0500
Subject: [Microscopy] Re: Cambridge S-250

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Skage,

We have been running an older version, S200 for some 15 years. Overall, it
has had an excellent service history (mostly replacing vacuum pumps and
occasional chips, resistors, capacitors, etc).

We are a metallurgy lab, so our samples tend to be conductive and dry. The
large sample chamber is a real plus as is the nice stage. Also, we are not
heavy users and kept limited access with regard to number of users. We do
miss having direct digital imaging, which I presume the S250 has. Your
situation may be very different than ours. Newer scopes offer partial
vacuum capabilities and probably better ultimate resolution.

Other than being an older instrument (not always a bad thing) without
some of the newer bells and whistles, I have no regrets and we look forward
to many more years of service from it.

Alan Stone
ASTON




At 10:07 AM 8/3/2005, you wrote:



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Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com


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From: GBurgess-at-exchange.hsc.mb.ca
Date: Wed, 3 Aug 2005 13:35:20 -0500
Subject: [Microscopy] best knife angle

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I had known at one time, but I suppose due to age, I can't remember, but I'm
hoping that people here could advise me as to the best knife angle to buy on
a Diatome knife. All our specimens are human biopsy tissue embedded in
epon. I'd like to buy a histo-knife, and I was under the impression that 45
degrees would be the best sort of knife angle for routine use like this. Is
this correct?

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

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From: leswes-at-shaw.ca
Date: Wed, 3 Aug 2005 13:51:42 -0500
Subject: [Microscopy] Re: Osmium vapors

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In North America, Kilner jars are called Mason jars.

--
Lesley Weston


} From: David.Patton-at-uwe.ac.uk
} Reply-To: microscopy-at-microscopy.com
} Date: Wed, 03 Aug 2005 06:26:13 -0500
} To: leswes-at-shaw.ca
} Subject: [Microscopy] Osmium vapors
}
}
}
}
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} Osmium vapours are not stopped by simple bottle tops. We buy ours
} already made up. The bottle lids have a plastic (?) insert which is
} effective.
}
} Waste osmium is stored in any bottle which is then kept in what we can
} in the UK a Kilner jar. It is made of glass, has a rubber seal and uses
} wire to clamp the lid very tightly. Osmium vapours do not seem to pass
} through rubber seals. Unfortunately new Kilner jars in my supermarket
} have plastic seals - so an experiment will be required.
}
} Dave
}
} -----Original Message-----
} X-from: gunkelrl-at-slu.edu [mailto:gunkelrl-at-slu.edu]
} Sent: 02 August 2005 17:49
} To: David Patton
} Subject: [Microscopy] Re: Osmium vapors
}
}
}
}
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} We use Osmium in our lab and keep it in the refrigerator.
} We keep it in a glass bottle and cover the lid with
} parafilm. Then we put that bottle in another bottle with a
} screw top and wrap the bottle in foil. It works great, our
} refrigerator doesn't have and black residue, just the two
} bottles.
}
} rebecca
}
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From: Elliott-at-arizona.edu
Date: Wed, 3 Aug 2005 13:58:44 -0500
Subject: [Microscopy] Re: best knife angle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I use a 45 for normal work. I find it works very well. I have a 35 I
use for special work. 35 has some advantages, but is not as robust.
David


On Aug 3, 2005, at 11:41 AM, GBurgess-at-exchange.hsc.mb.ca wrote:

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} I had known at one time, but I suppose due to age, I can't remember,
} but I'm
} hoping that people here could advise me as to the best knife angle to
} buy on
} a Diatome knife. All our specimens are human biopsy tissue embedded in
} epon. I'd like to buy a histo-knife, and I was under the impression
} that 45
} degrees would be the best sort of knife angle for routine use like
} this. Is
} this correct?
}
} This e-mail and/or any documents in this transmission is intended for
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From: hoffpajo-at-yahoo.com
Date: Wed, 3 Aug 2005 14:31:27 -0500
Subject: [Microscopy] Re: best knife angle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

it has been a while since i have purchased a knife. 48
degrees should work well for biological tissue.
if you have doubts, questions or concerns i recommend
contacting staci kirsh at electron microscopy
sciences, she is an invaluble resource.
john

--- GBurgess-at-exchange.hsc.mb.ca wrote:

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}
} I had known at one time, but I suppose due to age, I
} can't remember, but I'm
} hoping that people here could advise me as to the
} best knife angle to buy on
} a Diatome knife. All our specimens are human biopsy
} tissue embedded in
} epon. I'd like to buy a histo-knife, and I was
} under the impression that 45
} degrees would be the best sort of knife angle for
} routine use like this. Is
} this correct?
}
} This e-mail and/or any documents in this
} transmission is intended for the address(s) only and
} may contain legally privileged or confidential
} information. Any unauthorized use, disclosure,
} distribution, copying or dissemination is strictly
} prohibited. If you receive this transmission in
} error, please notify the sender immediately and
} return the original.
}
} ==============================Original
} Headers==============================
} 3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Wed Aug 3
} 13:35:20 2005
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} 3, 20 -- From: Garry Burgess
} {GBurgess-at-exchange.hsc.mb.ca}
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From: phillipst-at-missouri.edu
Date: Wed, 3 Aug 2005 14:35:43 -0500
Subject: [Microscopy] Knife angle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have had 45 degree diamonds for most of my career but last year bought a
35 degree one. I think there is a significant improvement for the lymphoid
tissue we are cutting these days. I hear they wear out faster but I don't
do a ton of sectioning so the tradeoff seems worth it. Tom



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: lambert-at-jeol.com
Date: Wed, 3 Aug 2005 14:36:25 -0500
Subject: [Microscopy] best knife angle

Contents Retrieved from Microscopy Listserver Archives
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I have received your message, but will be out of the office until August 4th and will not be able to reply until then.

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From: lambert-at-jeol.com
Date: Wed, 3 Aug 2005 14:58:57 -0500
Subject: [Microscopy] Accidental messages

Contents Retrieved from Microscopy Listserver Archives
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lambert-at-jeol.com wrote:

} I have received your message, but will be out of the office

I apologize to the list for the unnecessary messages. We have recently started using the automated reply feature of our email server with some unexpected results. Attempts to repair the auto-reply logic have (so far) failed. Hopefully, my unsubscribe request will be processed soon. Again, I apologize for wasted bandwidth.

Best regards,
Mike Lambert

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From: kenconverse-at-qualityimages.biz
Date: Wed, 3 Aug 2005 17:04:21 -0500
Subject: [Microscopy] Cambridge S-250

Contents Retrieved from Microscopy Listserver Archives
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Skage,
It might also depend on what you're trading for it. Some older SEMs might
be worth trading while others might be better kept in your lab.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 S. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 11:10 AM
To: kenconverse-at-qualityimages.biz

Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem







_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html



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31, 23 -- Subject: RE: [Microscopy] Cambridge S-250
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From: shem-at-laurentian.ca
Date: Wed, 3 Aug 2005 19:39:55 -0500
Subject: [Microscopy] Cambridge S-250

Contents Retrieved from Microscopy Listserver Archives
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Hi Ken, Its replacing a nanolab-7 (old Zeiss)
Skage

} } } kenconverse-at-qualityimages.biz 08/03/05 6:08 PM } } }



----------------------------------------------------------------------------
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Skage,
It might also depend on what you're trading for it. Some older SEMs might
be worth trading while others might be better kept in your lab.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 S. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 11:10 AM
To: kenconverse-at-qualityimages.biz

Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem







_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html



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31, 23 -- From kenconverse-at-qualityimages.biz Wed Aug 3 17:04:21 2005
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31, 23 -- Subject: RE: [Microscopy] Cambridge S-250
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From: hoffpajo-at-yahoo.com
Date: Thu, 4 Aug 2005 09:14:48 -0500
Subject: [Microscopy] best knife angle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Diatome suggest for their 'ultra' diamond knives, that 45 degree is
good for routine sections. 35 degree apparently offers less section
distortions in biology and materials science. 55 degree is best for
really hard ceramics and similar materials

The Diatome article offers two references:
1. J.C. Jesior; How to avoid compression; Jnl. of Ultrastructure &
Molecular Structure Res. 95, 210-217 (1986)
2. J.C. Jesior; Use of low-angle diamond knives leads to improved
ultrastructural preservation of ultrathin sections; Scanning Microscopy
Supplement 3, 147-153 (1989); Scanning Microscopy International,
Chicago (AMF O'Hare) IL 6066 USA.

This information came out of a Diatome colour brochure which I must
have received some time in the last 10 years or so. I do not have the
actual references.

NB Incidentally Diatome do market a 'Histo' knife but it is really
intended for light microscopy sections rather than ultrathin ones
('Ultra' knives).

Good luck

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: GBurgess-at-exchange.hsc.mb.ca

unless i have forgotten, diatome also/used to market a
semi diamone kife that would cut semi thin sections as
well as thin sections. of course the memory is the
first thing to go.

--- malcolm.haswell-at-sunderland.ac.uk wrote:

}
}
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}
} Diatome suggest for their 'ultra' diamond knives,
} that 45 degree is
} good for routine sections. 35 degree apparently
} offers less section
} distortions in biology and materials science. 55
} degree is best for
} really hard ceramics and similar materials
}
} The Diatome article offers two references:
} 1. J.C. Jesior; How to avoid compression; Jnl. of
} Ultrastructure &
} Molecular Structure Res. 95, 210-217 (1986)
} 2. J.C. Jesior; Use of low-angle diamond knives
} leads to improved
} ultrastructural preservation of ultrathin sections;
} Scanning Microscopy
} Supplement 3, 147-153 (1989); Scanning Microscopy
} International,
} Chicago (AMF O'Hare) IL 6066 USA.
}
} This information came out of a Diatome colour
} brochure which I must
} have received some time in the last 10 years or so.
} I do not have the
} actual references.
}
} NB Incidentally Diatome do market a 'Histo' knife
} but it is really
} intended for light microscopy sections rather than
} ultrathin ones
} ('Ultra' knives).
}
} Good luck
}
} Malcolm
}
} Malcolm Haswell
} e.m. unit
} School of Health, Natural and Social Sciences
} Fleming Building
} University of Sunderland
} Tyne & Wear
} SR1 3SD
} UK
} e-mail: malcolm.haswell-at-sunderland.ac.uk
}
}
} ----- Original Message -----
} X-from: GBurgess-at-exchange.hsc.mb.ca
} Date: Wednesday, August 3, 2005 7:41 pm
} Subject: [Microscopy] best knife angle
}
} }
} }
} }
} }
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} }
} }
} } I had known at one time, but I suppose due to age,
} I can't
} } remember, but I'm
} } hoping that people here could advise me as to the
} best knife angle
} } to buy on
} } a Diatome knife. All our specimens are human
} biopsy tissue
} } embedded in
} } epon. I'd like to buy a histo-knife, and I was
} under the
} } impression that 45
} } degrees would be the best sort of knife angle for
} routine use like
} } this. Is
} } this correct?
} }
} } This e-mail and/or any documents in this
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} } immediately and return the original.
} }
} } ==============================Original
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} 2005
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} } 3, 20 -- From: Garry Burgess
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}
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From: kenconverse-at-qualityimages.biz
Date: Thu, 4 Aug 2005 09:29:41 -0500
Subject: [Microscopy] Cambridge S-250

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Skage,
I'm not really familiar with either model, but maybe someone on the list is
and can give you some recommendations. You might also try contacting Earl
Weltmer of Scanservice Corp.
earlw-at-sbcglobal.net
as he is familiar with more makes and models than I am.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
16 Creek Rd.
Delta, PA 17314
717-456-5491
Fax 717-456-7996
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: shem-at-laurentian.ca [mailto:shem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 8:44 PM
To: kenconverse-at-qualityimages.biz

Hi Ken, Its replacing a nanolab-7 (old Zeiss)
Skage

} } } kenconverse-at-qualityimages.biz 08/03/05 6:08 PM } } }



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Skage,
It might also depend on what you're trading for it. Some older SEMs might
be worth trading while others might be better kept in your lab.

Ken Converse

owner
QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 S. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz

-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, August 03, 2005 11:10 AM
To: kenconverse-at-qualityimages.biz

Hello Everyone
Our lab has been offered a refurbished
Cambridge S-250 electron microscope in exchange for
another SEM unit.

Do anyone have anyone have strong opinions of this instrument ?
or know of any advice I should heed before agreeing to such a trade in ?

Thanks ,

Skage Hem







_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html



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16, 17 -- From: "Skage Hem" {SHem-at-laurentian.ca}
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31, 23 -- Subject: RE: [Microscopy] Cambridge S-250
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From: hoffpajo-at-yahoo.com
Date: Thu, 4 Aug 2005 10:52:35 -0500
Subject: [Microscopy] Re: Mannie Steglich

Contents Retrieved from Microscopy Listserver Archives
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thanks for the info, mannie is still around and hasn't
retired yet. i just got an email from him. we went to
college together. i can't tell you the number of
friends and girl friends i lost touch with from
college. some i regret others i do not.
so are you heading to st martins?

--- microbill-at-mohawk.net wrote:

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} The last I knew he was at MD Anderson in Texas - but
} he may well have
} retired by now - see
} http://woodenwonderstx.com/About.html
}
}
}
}
} At 12:49 PM 8/2/2005, you wrote:
}
}
}
}
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} }
} } is mannie Mannie Steglich still around? haven't
} heard
} } from him in a while.
} } john
} }
} } --- hoffpajo-at-yahoo.com wrote:
} }
} } }
} } }
} } }
} } }
}
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} } }
} } } in the alternative and it may be more expensive
} and
} } } you may not want to go there. and if someone
} with a
} } } better memory than i do anymore, can correct me.
} i
} } } do
} } } remember seeing premade OsO4 in small amounts.
} use
} } } what you need, perhaps even batch the tissue
} } } samples.
} } } then throw out the unused in the waste.
} } } just a thought.
} } }
} } } --- dmclea-at-sandia.gov wrote:
} } }
} } } }
} } } }
} } } }
} } } }
} } }
}
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} ----------------------------------------------------------------------------
} } } }
} } } } Marc
} } } }
} } } } We store our osmium in a secondary
} container...a
} } } } large poly pro bottle
} } } } (capped) and then we bag that container in a
} } } plastic
} } } } Ziploc. It helps a
} } } } bit.
} } } }
} } } } Dorrance
} } } }
} } } } -----Original Message-----
} } } } X-from: marc.pypaert-at-yale.edu
} } } } [mailto:marc.pypaert-at-yale.edu]
} } } } Sent: Tuesday, August 02, 2005 9:10 AM
} } } } To: McLean, Dorrance
} } } } Subject: [Microscopy] Osmium vapors
} } } }
} } } }
} } } }
} } } }
} } } }
} } }
}
} ------------------------------------------------------------------------
} } } } ----
} } } } The Microscopy ListServer -- CoSponsor: The
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} http://www.microscopy.com/MicroscopyListserver/FAQ.html
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} ------------------------------------------------------------------------
} } } } ----
} } } }
} } } } Question to the list:
} } } }
} } } } Our local safety inspector is concerned about
} the
} } } } appearance of the
} } } } fridge where we stock our solution of osmium.
} As
} } } you
} } } } can expect the
} } } } white interior of the fridge has turned grey
} with
} } } } vapors of osmium over
} } } } the years. Although we are taking every
} } } precautions
} } } } to avoid leakage of
} } } } osmium vapor from its container, this is a
} problem
} } } } that I have observed
} } } } in every EM lab that I have worked in. Does
} } } someone
} } } } on the list have a
} } } } method to store osmium in such a way as to
} avoid
} } } any
} } } } escape of vapor?
} } } } Also, is there something we could put into
} fridge
} } } } that would trap osmium
} } } } vapors as they leak out? Thanks for your
} advise.
} } } }
} } } } Marc
} } } }
} } } } --
} } } } Marc Pypaert
} } } } Department of Cell Biology
} } } } Center for Cell and Molecular Imaging
} } } } Ludwig Institute for Cancer Research
} } } } Yale University School of Medicine
} } } } 333 Cedar Street, PO Box 208002
} } } } New Haven, CT 06520-8002
} } } } TEL 203-785 3681
} } } } FAX 203-785 7446
} } } }
} } } }
} } } } ==============================Original
} } } } Headers==============================
} } } } 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2
} } } } 11:09:11 2005 5, 18 --
} } } } Received: from BIOMED.MED.YALE.EDU
} } } } (biomed.med.yale.edu
} } } } [130.132.232.48])
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} (8.12.11/8.12.8)
} } } with
} } } } ESMTP id
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} } } } Aug 2005
} } } } 11:09:11 -0500
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From: chiphead-at-sbcglobal.net
Date: Thu, 4 Aug 2005 11:09:10 -0500
Subject: [Microscopy] Reminder on Replying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fellow listers:

Just a reminder that the "Reply" functionality of the
list has recently changed.

Currently, when you "Reply" to a message, the e-mail
is sent to the entire list, not just the original
sender. I believe that this is/was a biproduct of
Nestor's efforts to minimize SPAM (great job Nestor,
keep up the good work).

This is different than in the past, when a "Reply"
only went to the author of the post you were replying
to.

While this makes it easier to reply and have a post go
to the group, it also seems to have spawned a number
of messages there were meant for specific individuals,
but made their way to the entire group.

Not necessarily a problem, but in some cases it could
be...

John W. Raffensperger, Jr.
Beaver Dam, Wisconsin, USofA

==============================Original Headers==============================
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From: GBurgess-at-exchange.hsc.mb.ca
Date: Thu, 4 Aug 2005 12:07:32 -0500
Subject: [Microscopy] Tungsten Carbide Knives and Semi-thin Epon Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I noticed the Tungsten Carbide knives in a catalog, and wondered if this
sort of knife would be at all suitable to cut 0.7 micron sections of Epon
embedding biological material. Currently we are using histo-diamond knives,
but it is my dream to be able to use a more durable, cheaper knife that
gives the same result. I was just wondering if anyone has tried this, and
what sort of result they might get with epon. Or is it a stupid idea?

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

==============================Original Headers==============================
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3, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca}
3, 20 -- Subject: Tungsten Carbide Knives and Semi-thin Epon Sections
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From: malis-at-NRCan.gc.ca
Date: Thu, 4 Aug 2005 12:22:30 -0500
Subject: [Microscopy] Knife angle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Although I am only familiar with so-called 'hard' materials microtomy
(metals, alloys, minerals, ceramic fibers and the like) the interesting
point about the knife angle in this area is that the 35 sections better for
all of the above materials in my lab, so the 45 knives gather dust. Since
wear is a function of the amount of sectioning as well as type of material,
I cannot comment on that aspect, but can note that we never had any damage
to the 35 except when we deliberately tried to do so on ~20 micron,
extremely hard amorphous alloys particles. (It was not pretty!).

The success of the lower angle is no fluke, as Helmut Gnaegi of Diatome has
gotten good sections of; 200 micron quartz particles, polysilicon/metal
layers on a single crystal silicon substrate, carbon fibers (but care had to
be used or nicks would occur), superconducting oxide and Ti implants in
bone. Phil Swab (with a coatings company in California) has sectioned many
optical glass coatings, and layers of boron nitride and artificial diamond
on single crystal silicon substrate. To the best of my knowledge, both of
these specialists had trouble with any other angles.

My theory is that, for most of the above examples, the knife edge only
initiates a crack in the fairly brittle material which is then 'wedged open'
across the block face by the angle of the knife, hence the reduced damage
with reduced angle (though section breakup does occur).

Finally, we did a bit of fooling around with histo knives a few years back.
Far from being only good for semithins, though 1 micron thick sections of
aluminum were produced, they produced some of the thinnest, flattest
sections we ever obtained (~10 nm for nanocrystalline metals) and was the
only knife that produced decent ~20 nm thick sections of the above amorphous
alloy particles. Go figure.

Tom

Dr. Tom Malis
Manager / Gestionnaire
Academic User Access Facility (AUAF) / La FacilitïŠ d’accïs aux utilisateurs
universitaire (FAUU)
CANMET Materials Technology Laboratory / LTM-CANMET
Natural Resources Canada / Ressources naturelles Canada
568 Booth St. / 568 rue Booth, Ottawa, Ontario K1A 0G1
Tel.: (613) 995-1493 FAX: (613) 992-8735; cell: 613-371-4577
malis-at-nrcan.gc.ca

-----Original Message-----
X-from: phillipst-at-missouri.edu [mailto:phillipst-at-missouri.edu]
Sent: August 3, 2005 3:38 PM
To: malis-at-nrcan.gc.ca

I have had 45 degree diamonds for most of my career but last year bought a
35 degree one. I think there is a significant improvement for the lymphoid
tissue we are cutting these days. I hear they wear out faster but I don't
do a ton of sectioning so the tradeoff seems worth it. Tom



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: hoffpajo-at-yahoo.com
Date: Thu, 4 Aug 2005 12:23:53 -0500
Subject: [Microscopy] Re: Tungsten Carbide Knives and Semi-thin Epon Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

the histo knife should last for years in the hands of
a careful tech. and if you are using the sections for
orientation a few knife scratches should matter. just
be sure to not let an inexperienced person use them.

--- GBurgess-at-exchange.hsc.mb.ca wrote:

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} I noticed the Tungsten Carbide knives in a catalog,
} and wondered if this
} sort of knife would be at all suitable to cut 0.7
} micron sections of Epon
} embedding biological material. Currently we are
} using histo-diamond knives,
} but it is my dream to be able to use a more durable,
} cheaper knife that
} gives the same result. I was just wondering if
} anyone has tried this, and
} what sort of result they might get with epon. Or is
} it a stupid idea?
}
} This e-mail and/or any documents in this
} transmission is intended for the address(s) only and
} may contain legally privileged or confidential
} information. Any unauthorized use, disclosure,
} distribution, copying or dissemination is strictly
} prohibited. If you receive this transmission in
} error, please notify the sender immediately and
} return the original.
}
} ==============================Original
} Headers==============================
} 3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Thu Aug 4
} 12:07:31 2005
} 3, 20 -- Received: from hscxntmx0003.hsc.mb.ca
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} 3, 20 -- Date: Thu, 4 Aug 2005 12:06:02 -0500
} 3, 20 -- From: Garry Burgess
} {GBurgess-at-exchange.hsc.mb.ca}
} 3, 20 -- Subject: Tungsten Carbide Knives and
} Semi-thin Epon Sections
} 3, 20 -- To: "'microscopy-at-microscopy.com'"
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From: Michael_Standing-at-byu.edu
Date: Thu, 4 Aug 2005 12:58:58 -0500
Subject: [Microscopy] Tungsten Carbide Knives and Semi-thin Epon Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have used the Tungsten Carbide knives some. They are not sharp enough to
cut sub-micron thick sections as they leave a slight snowy appearance to the
block face. I have been successful in cutting plastic embedded tissue
sections of approximately 3-5 microns with them. The edge stays good much
longer than glass does. They are great for working with harder materials.
After applying per-mount and coversliping the sections, the snowiness is no
longer evident.

===========================================
Michael D. Standing
Microscopy Technician
Brigham Young University Microscopy Lab
A-125A CLFB
Provo, UT 84602

Phone: (801) 422-4011
E-Mail: Michael_Standing-at-byu.edu
===========================================

-----Original Message-----
X-from: GBurgess-at-exchange.hsc.mb.ca [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Thursday, August 04, 2005 11:09 AM
To: Michael_Standing-at-byu.edu


I noticed the Tungsten Carbide knives in a catalog, and wondered if this
sort of knife would be at all suitable to cut 0.7 micron sections of Epon
embedding biological material. Currently we are using histo-diamond knives,
but it is my dream to be able to use a more durable, cheaper knife that
gives the same result. I was just wondering if anyone has tried this, and
what sort of result they might get with epon. Or is it a stupid idea?

This e-mail and/or any documents in this transmission is intended for the
address(s) only and may contain legally privileged or confidential
information. Any unauthorized use, disclosure, distribution, copying or
dissemination is strictly prohibited. If you receive this transmission in
error, please notify the sender immediately and return the original.

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==============================Original Headers==============================
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12, 21 -- To: {microscopy-at-microscopy.com}
12, 21 -- Subject: RE: [Microscopy] Tungsten Carbide Knives and Semi-thin Epon Sections
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From: wesaia-at-iastate.edu
Date: Thu, 4 Aug 2005 13:09:47 -0500
Subject: [Microscopy] Re: Reminder on Replying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Another couple reminder about recent changes-

First, it used to be that a poster's personal name would appear in the
X-from: field and give a pretty good idea of their identity. (List rules
(http://www.msa.microscopy.org/MicroscopyListserver/Rules.html) ask that we
provide our real name as part of the post.) Recent changes have stripped
that field down to just the e-mail address. That makes messages look a lot
more anonymous than before. In fact, the original From: field is preserved
in the new stuff tacked on to the bottom of the post, but many of us
probably don't look those lines over. So, it is probably a good practice to
add a brief signature line so we clearly know who you are and can avoid
some of the recent confusion.

Second, there are those new lines of stuff tacked onto the end of the
posts. I encourage you all to trim those back as you reply to messages.
They add unnecessary bulk to the messages. A new set is always tacked on
anyway.

Cheers, even to those of you in Hawaii. I almost joined you there.

Warren Straszheim
Iowa State University

At 11:11 AM 08/04/05, you wrote:

} Fellow listers:
}
} Just a reminder that the "Reply" functionality of the
} list has recently changed.
}
} Currently, when you "Reply" to a message, the e-mail
} is sent to the entire list, not just the original
} sender. I believe that this is/was a biproduct of
} Nestor's efforts to minimize SPAM (great job Nestor,
} keep up the good work).
}
} This is different than in the past, when a "Reply"
} only went to the author of the post you were replying
} to.
}
} While this makes it easier to reply and have a post go
} to the group, it also seems to have spawned a number
} of messages there were meant for specific individuals,
} but made their way to the entire group.
}
} Not necessarily a problem, but in some cases it could
} be...
}
} John W. Raffensperger, Jr.
} Beaver Dam, Wisconsin, USofA





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From: rcbaker-at-eden.infohwy.com
Date: Thu, 4 Aug 2005 13:34:10 -0500
Subject: [Microscopy] Tungsten Carbide Knives and Semi-thin Epon Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Aug 4, 2005, at 1:03 PM, Michael_Standing-at-byu.edu wrote:

}
}
}
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} America
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}
} I have used the Tungsten Carbide knives some. They are not sharp
} enough to
} cut sub-micron thick sections as they leave a slight snowy
} appearance to the
} block face. I have been successful in cutting plastic embedded tissue
} sections of approximately 3-5 microns with them. The edge stays
} good much
} longer than glass does. They are great for working with harder
} materials.
} After applying per-mount and coversliping the sections, the
} snowiness is no
} longer evident.
}
} ===========================================
} Michael D. Standing
} Microscopy Technician
} Brigham Young University Microscopy Lab
} A-125A CLFB
} Provo, UT 84602
}
} Phone: (801) 422-4011
} E-Mail: Michael_Standing-at-byu.edu

Exactly, Michael.

The problem with tungsten carbide is that it is actually a
microcrystalline alloy of tungsten carbide particles bonded with
cobalt, and this microstructure structure interferes with very thin
sections, much as with steel, but the carbide is harder and tougher,
which is why it is used to machine steel.

Knife facets may look quite shiny but they need to be polished much
flatter than a wavelength of visible light if they are to meet at a
smooth edge measured in the low nanometers, and thus be capable of
cutting the sub-100 nanometer sections appropriate for EM work.

The good thing about metal alloys is that the edge angle can be made
more acute while retaining the desired durability, but only at the
expense of minimum section thickness, so they are used for LM work.

Glass does well for EM knives because it is amorphous and thus has no
microstructure, while being hard enough to section. Of the hard
monocrystal alternatives to glass suitable for EM knives, only
sapphire, silicon carbide and diamond seem appropriate. All these are
good candidate materials for ultramicrotome knives, depending on cost
and other requirements.

Roger Baker
1303 Bentwood,
Austin, Tx,
78722





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From: cpetty1-at-umbc.edu
Date: Thu, 4 Aug 2005 14:51:06 -0500
Subject: [Microscopy] Phil Rutledge

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
I am looking for a tech that used to work here at UMBC, his name is
Phil Rutledge. If anyone knows his whereabouts could you have him e-mail
me at cpetty1-at-umbc.edu. He is a whiz with both our zeiss and jeol and I
would love to ask him some questions about the scopes and stuff I have
found lying about the facility.
Thanks
--
Chere Petty, M.S.
Manager, Keith R. Porter Imaging Facility
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
1000 Hilltop Circle
Baltimore, MD 21250
Phone: 410-455-2296
Fax: 410-455-3875

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From: hoffpajo-at-yahoo.com
Date: Fri, 5 Aug 2005 09:53:47 -0500
Subject: [Microscopy] Tungsten Carbide Knives and Semi-thin Epon Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Glass Knives, cheap fast, reliable, Histo Diamonds are excellent and durable
if used with care!
Markus F. Meyenhofer
Microscopy Labs.
----- Original Message -----
X-from: {GBurgess-at-exchange.hsc.mb.ca}
To: {micro-at-superlink.net}
Sent: Thursday, August 04, 2005 1:07 PM

yes i agree, glass knives are relativly cheap, fast?
depends on what you are using them for. histo knives
should last for years and can be used to face the
blocks as well. used one for years untill someone with
little experience got hold of it, i had never seen a
diamond with so many chips in it.
moral of the story, keep your knives close, but keep
your diamind knives to yourself.

--- micro-at-superlink.net wrote:

}
}
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}
} Glass Knives, cheap fast, reliable, Histo Diamonds
} are excellent and durable
} if used with care!
} Markus F. Meyenhofer
} Microscopy Labs.
} ----- Original Message -----
} X-from: {GBurgess-at-exchange.hsc.mb.ca}
} To: {micro-at-superlink.net}
} Sent: Thursday, August 04, 2005 1:07 PM
} Subject: [Microscopy] Tungsten Carbide Knives and
} Semi-thin Epon Sections
}
}
} }
} }
} }
} }
}
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} }
} }
} } I noticed the Tungsten Carbide knives in a
} catalog, and wondered if this
} } sort of knife would be at all suitable to cut 0.7
} micron sections of Epon
} } embedding biological material. Currently we are
} using histo-diamond
} } knives,
} } but it is my dream to be able to use a more
} durable, cheaper knife that
} } gives the same result. I was just wondering if
} anyone has tried this, and
} } what sort of result they might get with epon. Or
} is it a stupid idea?
} }
} } This e-mail and/or any documents in this
} transmission is intended for the
} } address(s) only and may contain legally privileged
} or confidential
} } information. Any unauthorized use, disclosure,
} distribution, copying or
} } dissemination is strictly prohibited. If you
} receive this transmission in
} } error, please notify the sender immediately and
} return the original.
} }
} } ==============================Original
} } Headers==============================
} } 3, 20 -- From GBurgess-at-exchange.hsc.mb.ca Thu Aug
} 4 12:07:31 2005
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} } [142.233.100.122])
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} } 3, 20 -- Date: Thu, 4 Aug 2005 12:06:02 -0500
} } 3, 20 -- From: Garry Burgess
} {GBurgess-at-exchange.hsc.mb.ca}
} } 3, 20 -- Subject: Tungsten Carbide Knives and
} Semi-thin Epon Sections
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From: gvrdolja-at-nature.berkeley.edu
Date: Fri, 5 Aug 2005 12:35:48 -0500
Subject: [Microscopy] Re: Osmium vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We use bottles with rubber septums to store the liquid, and keep the
bottles within sealed falcon tubes, within a sealed bottle. This keeps
the vapours from leaking and discolouring the fridge.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Tue, 2 Aug 2005, marc.pypaert-at-yale.edu wrote:

}
}
}
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} ----------------------------------------------------------------------------
}
} Question to the list:
}
} Our local safety inspector is concerned about the appearance
} of the fridge where we stock our solution of osmium. As you
} can expect the white interior of the fridge has turned grey
} with vapors of osmium over the years. Although we are
} taking every precautions to avoid leakage of osmium
} vapor from its container, this is a problem that I have
} observed in every EM lab that I have worked in. Does
} someone on the list have a method to store osmium in such
} a way as to avoid any escape of vapor? Also, is there
} something we could put into fridge that would trap osmium
} vapors as they leak out? Thanks for your advise.
}
} Marc
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
} ==============================Original Headers==============================
} 5, 18 -- From marc.pypaert-at-yale.edu Tue Aug 2 11:09:11 2005
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} 5, 18 -- Date: Tue, 02 Aug 2005 12:09:07 -0400
} 5, 18 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 5, 18 -- Subject: Osmium vapors
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}

==============================Original Headers==============================
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From: dmyates-at-seas.upenn.edu
Date: Fri, 5 Aug 2005 15:58:30 -0500
Subject: [Microscopy] Microscopist Position Available - Univ Penn

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listservers,

The Penn Regional Nanotechnology Facility at the University of
Pennsylvania has an opening for a Research Scientist/Microscopist. The
facility is equipped with transmission and scanning electron microscopes,
atomic force microscopes, an ion accelerator and a focused ion beam. The
successful candidate will assist the facility's technical director in
training and assisting users, developing methods and maintaining the
instruments.
Qualifications for this position include an M.S. in materials
or other physical science (Ph.D. preferred). A minimum of three years
experience in the operation of SEM, TEM, FIB or AFM (experience with FIB or
AFM preferred).

For immediate consideration, send resume/cv to:

Doug Yates
dmyates-at-lrsm.upenn.edu
Penn Regional Nanotechnology Facility
University of Pennsylvania
3231 Walnut Street
Philadelphia, PA 19104

Additional information about the position may be viewed at:
http://www.hr.upenn.edu/jobs
reference number: 050818006

Information about the Penn Regional Nanotechnology Facility may be viewed at:
http://www.seas.upenn.edu/nanotechfacility

Thanks,
Doug Yates

==============================Original Headers==============================
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7, 28 -- Date: Fri, 5 Aug 2005 16:58:18 -0400
7, 28 -- From: dmyates-at-seas.upenn.edu
7, 28 -- To: Microscopy-at-microscopy.com
7, 28 -- Subject: Microscopist Position Available - Univ Penn
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From: schenderson-at-vcu.edu
Date: Fri, 5 Aug 2005 17:59:30 -0500
Subject: [Microscopy] Microscopy Technician position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



==============================Original Headers==============================
2, 21 -- From schenderson-at-vcu.edu Fri Aug 5 17:59:29 2005
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2, 21 -- Reply-To: {schenderson-at-vcu.edu}
2, 21 -- From: "Scott Henderson" {schenderson-at-vcu.edu}
2, 21 -- To: {Microscopy-at-microscopy.com}
2, 21 -- Subject: Microscopy Technician position available
2, 21 -- Date: Fri, 5 Aug 2005 18:59:35 -0400
2, 21 -- Organization: Virginia Commonwealth University
2, 21 -- MIME-Version: 1.0
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==============================End of - Headers==============================




From: schenderson-at-vcu.edu
Date: Fri, 5 Aug 2005 18:08:01 -0500
Subject: [Microscopy] Microscopy Technician position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The following technical position is available at Virginia Commonwealth
University School of Medicine.

Microscopy Technician (Position # 55122)  

A technical position is available in the Microscopy Facility of the
Department of Anatomy and Neurobiology in the School of Medicine at Virginia
Commonwealth University. The facility houses confocal, multi-photon,
fluorescence, and electron microscopes (TEM & SEM).  The successful
candidate will assist with microscopy studies of various biological
systems.  Duties include instructing and assisting users of the facility,
sample preparation, image analysis and minor equipment maintenance. 
Applicants should have excellent communication and organizational skills, an
understanding of basic laboratory procedures, and the ability to manage a
large and varied workload. Qualifications include a degree in Biology/Life
Sciences, at least 2 years of experience with laser scanning microscopy
(confocal and/or multi-photon), sample preparation, digital imaging, and
image analysis.  Experience with electron microscopy is an asset. Computer
skills are essential. 

Applications are to be submitted online via the VCU Jobs website at:

www.vcujobs.com

Click on the “Search Postings” link and under the “Working Title” field,
select “Microscopy Technician”

--------------------------

Scott Henderson, Ph.D.
Director of Microscopy
Associate Professor
Dept. Anatomy & Neurobiology
Virginia Commonwealth University
School of Medicine
Sanger Hall, Rm. 9-069d
1101 East Marshall St.
Richmond, VA 23298-0709



==============================Original Headers==============================
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9, 22 -- Reply-To: {schenderson-at-vcu.edu}
9, 22 -- From: "Scott Henderson" {schenderson-at-vcu.edu}
9, 22 -- To: {Microscopy-at-microscopy.com}
9, 22 -- Subject: Microscopy Technician position available
9, 22 -- Date: Fri, 5 Aug 2005 19:08:06 -0400
9, 22 -- Organization: Virginia Commonwealth University
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From: ken.blight-at-cancer.org.uk
Date: Fri, 5 Aug 2005 19:33:06 -0500
Subject: [Microscopy] viaWWW: Drosophila eye ultrathin cryosections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ken.blight-at-cancer.org.uk) from
http://microscopy.com/MLFormMail.html on Wednesday, August 3, 2005 at
10:40:02
---------------------------------------------------------------------------

Email: ken.blight-at-cancer.org.uk
Name: Ken Blight

Organization: cancer research uk

Title-Subject: [Filtered] Drosophila eye ultrathin cryosections

Question: I have processed Drosophila eyes for immunolabelling (EM)
using the Tokuyasu technique and the results are, to say the least,
very disappointing. The eyes floated in the fixative which didn't
help matters. The problem looks to be spacial when I compare the
results to standard resin TEM preparation. Maybe there is some
problem with buffers, fixation or section expansion. Does anyone
have experience of these tough little beasties?

---------------------------------------------------------------------------

==============================Original Headers==============================
6, 12 -- From zaluzec-at-microscopy.com Fri Aug 5 19:33:05 2005
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: ken.blight-at-cancer.org.uk (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Drosophila eye ultrathin cryosections
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: cornheadorama-at-hotmail.com
Date: Fri, 5 Aug 2005 19:33:27 -0500
Subject: [Microscopy] viaWWW: Cambridge S250

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cornheadorama-at-hotmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, August 3, 2005 at 18:07:30
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver: Re: Cambridge S250

Question: Hi,

I'm not too familiar with these despite the fact I have two of them
gathering dust. But I have heard them disfavorably compared to the
S200. Mine may be site-rigged for handling semi wafers (and there's a
chance both came from the same facility, at different times... a
hunch) but in mine the stage uses little more substantial than pvc
tubing to transmit the z-axis knob to the stage: horrible hysteresis.

I suspect these and related scopes were enormously popular, and I
know for a fact this 'scope has die-hard fans.

LaB6 is typical but it wouldn't surprise me if there was an active
aftermarket upgrade to FE: indeed one of mine may have one. On the
other hand, you may have missed the bulk of the aftermarket boat by
about 5 years: lots of parts for old scopes falling off of price
lists.... but sometimes onto "clearance" lists :)

But I'd say even if your own scope leaves a lot to be desired,
trading it for another old scope like this? I'd say deosn't quite
make sense unless you have a special requirement. Ultimately, it just
may not be worth the shipping: which of course is dicey unless
there's at least a few hours teardown and re-assembly. Better the
devil you know.

I hope more people respond, I've been back and forth as to whether to
try to get one or both of mine working for about 6 years now :)

-Jeff


---------------------------------------------------------------------------

==============================Original Headers==============================
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13, 12 -- To: microscopy-at-microscopy.com
13, 12 -- From: cornheadorama-at-hotmail.com (by way of MicroscopyListserver)
13, 12 -- Subject: viaWWW: Cambridge S250
13, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: cornheadorama-at-hotmail.com
Date: Fri, 5 Aug 2005 19:33:55 -0500
Subject: [Microscopy] viaWWW: Osmium Vapors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (cornheadorama-at-hotmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Wednesday, August 3, 2005 at 18:41:52
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver:b Re: Osmium Vapors

Question: I don't have experience with osmium compounds, and my
suggestion may not be very practical (read: expensive) unless you
have piles of vacuum fittings lying around like I do, but on occasion
when wanting to "absolutely" seal something up I've used a KF50
stainless nipple (or bellows, sometimes)of appropriate length, a pair
of stainless blanks, clamps, o-rings etc. so that the item could be
sealed "vacuum tight". Of course it's all relative but I wouldn't
think much vapour will diffuse though the ~5mm of highly compressed
Viton. The clamps are a bit clumsy to put on but very quick to come
off: I'm not sure you can get a quick-release clamp in KF50 but that
would make it almost one-handed. You could probably save money and
thru-gassing by having a single-ended arrangment, ie: a pipe section
with a KF50 flange welded on one end and a cap welded on the other.
Some (if not most) of the hi-vac houses weld their standard catalog
items on-site, and some don't charge much extra (~%20 premium) for
custom items from stock parts.

I think the idea of absorbants/reactants holds a lot of potential.
Activated charcoal comes immediately to mind.
Is it so reactive as to present a fire hazard if quickly adsorbed
onto the huge surface area?

-Jeff

---------------------------------------------------------------------------

==============================Original Headers==============================
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8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: cornheadorama-at-hotmail.com (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Osmium Vapors
8, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: omelon-at-geology.utoronto.ca
Date: Fri, 5 Aug 2005 19:34:15 -0500
Subject: [Microscopy] viaWWW: further hardening of LR White

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (omelon-at-geology.utoronto.ca) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on
Thursday, August 4, 2005 at 16:57:53
---------------------------------------------------------------------------

Email: omelon-at-geology.utoronto.ca
Name: Christopher R. Omelon

Organization: University of Toronto

Title-Subject: [Filtered] further hardening of LR White

Question: I have tried embedding large samples (~ 1cm3) of porous
rock material in a variety of resins and find that LR White (Hard)
provides the best infilling due to its low viscosity. That being
said, the blocks are not as hard as when using epoxide resins such as
EMBED 812. I am cold-curing (i.e. with accelerator) at both room
temperature and at 4?C with mixed results, but even in the best case
the blocks are not as hard as I would expect. My question: can I
further harden the blocks by now placing these samples in the oven at
60?C? My reasoning is that resin within the block (i.e. beneath the
surface and therefore not exposed to oxygen) will proceed with
polymerization at a faster rate than if I left the blocks at room
temperature. Or is the reaction now at completion? Thanks

---------------------------------------------------------------------------

==============================Original Headers==============================
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6, 12 -- To: microscopy-at-microscopy.com
6, 12 -- From: omelon-at-geology.utoronto.ca (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: further hardening of LR White
6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
==============================End of - Headers==============================




From: block-at-nova.edu
Date: Fri, 5 Aug 2005 19:35:01 -0500
Subject: [Microscopy] viaWWW: pseudo-confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (block-at-nova.edu) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
August 5, 2005 at 09:44:34
---------------------------------------------------------------------------

Email: block-at-nova.edu
Name: R. Block

Organization: NSU

Title-Subject: [Filtered] MListserver:

Question: Can you give me a source of information explaining pseudo-
confocal microscopy, how the data are acquired and analyzed?
Thanks in advance.

---------------------------------------------------------------------------

==============================Original Headers==============================
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6, 12 -- From: block-at-nova.edu (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: pseudo-confocal microscopy
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From: elke.buschbeck-at-uc.edu
Date: Fri, 5 Aug 2005 19:35:21 -0500
Subject: [Microscopy] viaWWW: Sodium Azide and TEM

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Email: elke.buschbeck-at-uc.edu
Name: Elke

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:Sodium Azide and TEM

Question: Does anyone know if it is o.k. to use sodium azide with TEM
preps? Does it wash out o.k. or will it leave a nasty deposit?
Thanks

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From: sdmegs09-at-yahoo.com
Date: Fri, 5 Aug 2005 19:35:46 -0500
Subject: [Microscopy] viaWWW: Energy dispersive spectroscopy fixation

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Email: sdmegs09-at-yahoo.com
Name: Meghan Donahue

Organization: University of San Diego

Title-Subject: [Filtered] MListserver: Energy dispersive spectroscopy fixation

Question: I am trying to fix plant material for EDS in the TEM and I
cannot find literature stating the fixation techniques. Is fixation
different for EDS?

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From: lauriern-at-videotron.ca
Date: Fri, 5 Aug 2005 19:36:15 -0500
Subject: [Microscopy] AskAMicroscopist: maximum resolution achievable in Phase Contrast

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Email: lauriern-at-videotron.ca
Name: Norm

Organization: None

Education: Graduate College

Location: Canada

Question: What is the maximum resolution achievable in Phase Contrast
and also Interferential Contrat DIC microscopy.
Since in bright field it is appr 1.40 I am sure that because of the
phase rings as well as the use of Wollaston prism in DIC, it must be
much lower even using oil immersion objective.


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From: dsherman-at-purdue.edu
Date: Sat, 6 Aug 2005 13:12:08 -0500
Subject: [Microscopy] Re: viaWWW: Sodium Azide and TEM

Contents Retrieved from Microscopy Listserver Archives
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Elka,
We use it routinely in buffers used to make up solutions for
immunolocalization on sections. I have never had a problem with
percipitation from sodium azide.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 8/5/05 7:41 PM, "elke.buschbeck-at-uc.edu" {elke.buschbeck-at-uc.edu} wrote:

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} Email: elke.buschbeck-at-uc.edu
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}
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}
} Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
}
} Question: Does anyone know if it is o.k. to use sodium azide with TEM
} preps? Does it wash out o.k. or will it leave a nasty deposit?
} Thanks
}
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From: frank.karl-at-degussa.com
Date: Mon, 8 Aug 2005 06:47:26 -0500
Subject: [Microscopy] Re: viaWWW: Sodium Azide and TEM

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One concern I have always heard was the formation of metal azide,
especially lead azide. These explosive and unstable have been found in
older metal plumbing. When I started as a QC chemist years ago, dilute
concentrations of sodium azoide was used as a perservative in some test
solutions used in clinical chemistry. One than one person got an
unpleasent surprize when working with the older style lead drain pipes. I
later wanted to use a test solution for sulfur which contained sodium
azide, my management had fits. I never did use that test.

I don't want to creat an electron storm of words, but I will suggest you
check out disposial method for your own safety.

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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elke.buschbeck-at-uc
.edu To: frank.karl-at-degussa.com
cc:
08/05/2005 08:38 Subject: [Microscopy] viaWWW: Sodium Azide and TEM
PM
Please respond to
microscopy








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Title-Subject: [Filtered] MListserver:Sodium Azide and TEM

Question: Does anyone know if it is o.k. to use sodium azide with TEM
preps? Does it wash out o.k. or will it leave a nasty deposit?
Thanks

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From: kirk-at-UDel.Edu
Date: Mon, 8 Aug 2005 07:20:14 -0500
Subject: [Microscopy] Re: Job Announcement

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Dear Microscopists,

We are looking to hire an Associate Scientist in our Bioimaging Center.
Although we place special emphasis on scanning probe microscopy, skills
in other areas of microscopy would be highly desirable. Please see the
job announcement below for additional details.

Best Regards, Kirk





*Associate Scientist – Bioimaging*

*University of Delaware*

*Delaware Biotechnology Institute*

The Delaware Biotechnology Institute (DBI) was established in 1999 as an
academic unit of the University of Delaware to position Delaware as a
leader in the life sciences. The Institute’s mission is to engage in
leading-edge scientific discovery in the life sciences, provide
biotechnology-based education, and promote economic development. The
major interdisciplinary research areas include human health,
agriculture, marine ecosystems and biomaterials.

The Institute functions as a partnership involving State government, the
Delaware institutions of higher education, and area industry, and is
housed in a new 72,000 ft2 state-of-the-art research facility. In
addition to individual research laboratories, it houses several core
facilities including a custom microarray center, a bioinformatics center
and a bioimaging center. The bioimaging center provides an array of
microscopy expertise and equipment, including conventional fluorescence,
confocal, multiphoton, atomic force, laser microdissection, transmission
and field emission scanning microscopes and ancillary sample preparation
equipment.

Under the limited direction of the Director of the Bioimaging Center,
the Associate Scientist independently interprets, organizes, executes,
and coordinates research assignments. Formulates and conducts research
on problems of considerable scope and complexity.

MAJOR RESPONSIBILITIES:

Provide consultation, training and supervision of graduate students,
post-doctoral fellows, staff and faculty in the design, implementation
and execution of microscopy-related projects with special emphasis on
scanning probe microscopy.

Interpret, organize, execute, and coordinate scientific research
assignments concerned with unique and highly complex problems.

Develop competitive research proposals to successfully generate external
research funding.

Collaborate with users and principal investigators on design, analysis,
application, and reporting of research projects; teach and advise on
techniques.

Design, perform, and/or oversee experiments, collect, analyze, and
interpret data, ensure data integrity, quality control, and protocol
compliance, prepare statistical and narrative reports and/or graphs.

Perform research assignments involving a number of variables, apply
diversified knowledge of scientific research principles, practices, and
protocols in research projects; make recommendations and conclusions
which serve as the basis for decision making.

Make authoritative decisions and recommendations that have a major
impact on scientific research activities and result in national and/or
international recognition.

Evaluate, select, and apply standard scientific techniques, procedures,
and criteria to accomplish a variety of research assignments.

Maintain a broad knowledge of state-of-the-art technology, software,
and/or systems.

Perform miscellaneous job-related duties as assigned


QUALIFICATIONS:

Master’s degree, Ph.D. preferred, in Biology/Chemistry or related field
as well as a record of peer reviewed publications and at least 5 years
demonstrated experience in scanning probe microscopy. Knowledge of
scientific approach, methodologies and scientific research principles,
practices and protocols in order to design, organize and coordinate
scientific research projects. Ability to perform independent original
research in an advanced area of scientific expertise. Ability to develop
scientific reports, proposals and publications on original research and
a knowledge of contemporary technological developments in the area of
scanning probe microscopy. Knowledge of the use and maintenance of
laboratory facilities and/or equipment. Ability to use independent
judgment to adapt and modify research concepts and approaches to
specific projects. Knowledge of current technological
developments/trends in area of scanning probe microscopy. Strong
communication, personal and organizational skills. Motivation to
learn/develop new techniques, flexibility, and ability to interact with
a diverse group of research personnel. approaches to specific projects.
Knowledge of current technological developments/trends in area of
scanning probe microscopy. Strong communication, personal and
organizational skills. Motivation to learn/develop new techniques,
flexibility, and ability to interact with a diverse group of research
personnel.

*Contact:* Interested candidates should forward curriculum vitae and the
names and contact information for three references to Dr. Kirk Czymmek,
15 Innovation Way, Suite 117, Delaware Biotechnology Institute,
University of Delaware, Newark, DE 19711 or by email (kirk-at-udel.edu).


Details can also be found at the following website:
http://www.dbi.udel.edu/career.html


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From: tivol-at-caltech.edu
Date: Mon, 8 Aug 2005 12:47:26 -0500
Subject: [Microscopy] Re: viaWWW: Energy dispersive spectroscopy fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Aug 5, 2005, at 5:36 PM, sdmegs09-at-yahoo.com wrote:

} Question: I am trying to fix plant material for EDS in the TEM and I
} cannot find literature stating the fixation techniques. Is fixation
} different for EDS?
}
Dear Meghan,
The preparation methods for any sample on which you are doing EDS or
other analytic techniques must take into account the elements you are
trying to analyze, so, for example, if you are looking for
water-soluble elements like Na or Cl ion, do not rinse the tissue in
solvents that will remove them. Note, however, that if you are looking
for Cl bound in organic compounds, such as polychlorinated biphenyls,
washing out the ion will enable you to distinguish the organochlorine.
For accurate quantitation, it is a good idea, if possible, to examine
the material untreated, except for freezing and perhaps sectioning,
then dehydrate by lyophylization in the scope and redo the analysis.
This will give accurate values for volatile elements and you can
improve the quantitation by taking the wet/dry ratios from elements
that are not affected by the drying process. Another consideration,
for the case that the elements of interest are not affected by your
preparation process, is not to use a stain that interferes with lines
from those elements. Pb and S are notorious for this, and one case I
ran into was trying to analyze Pt and Ir in a specimen that was treated
with Os and given to me. Since your specimens may not be suitable for
observation in the TEM when not prepared in some way, and since you may
not have access to cryopreparation methods, you need to keep in mind
the general principles that, whatever the preparation steps, they
cannot affect the concentration of the elements of interest, and cannot
interfere with the lines you want to measure. Good luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: bigelow-at-engin.umich.edu
Date: Mon, 8 Aug 2005 13:54:13 -0500
Subject: [Microscopy] TEM: RE: Beam stop & Faraday cup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A few weeks ago someone asked about a beam stop for a TEM.

Sopme time ago I designed a combination beam stop and Faraday cup for
a JEOL 2010 TEM. It has been in use for several years now, and I
understand has performed satisfactorily. If anyone is interested in
making use of the design, contact me.

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-662-5237
Address mail to: 1136 Mixtwood Rd.
Ann Arbor, MI 48103-3035

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From: jfb-at-uidaho.edu
Date: Mon, 8 Aug 2005 15:10:14 -0500
Subject: [Microscopy] TEM: RE: Beam stop & Faraday cup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

and a terrific boss!
:)

----- Original Message -----
X-from: schenderson-at-vcu.edu

Dr. Bigelow,
I would love to have use of the design for our 2010. Also, do you think it
could be altered to fit a 1200 EX-II?

-----Original Message-----
X-from: bigelow-at-engin.umich.edu [mailto:bigelow-at-engin.umich.edu]
Sent: Monday, August 08, 2005 11:58 AM
To: jfb-at-uidaho.edu

A few weeks ago someone asked about a beam stop for a TEM.

Sopme time ago I designed a combination beam stop and Faraday cup for
a JEOL 2010 TEM. It has been in use for several years now, and I
understand has performed satisfactorily. If anyone is interested in
making use of the design, contact me.

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-662-5237
Address mail to: 1136 Mixtwood Rd.
Ann Arbor, MI 48103-3035

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From: jmkrupp-at-cats.ucsc.edu
Date: Mon, 8 Aug 2005 17:48:38 -0500
Subject: [Microscopy] Looking for tiny holes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

A guy came into the lab today asking me to image and measure some holes in
a silicon nitride wafer.

OK, I say, how big?

2 nm he says, some maybe up to 70 nm.

That's pretty small, I said. How did you plan on doing it?

Don't know, that's why I came to you, says he.

Here is what he's got. A chip about 1 mm thick with an FIB section out of
it that is like an inverted pyramid into the chip. The pyramid doesn't go
all the way through, but there is a thin area at the bottom that is
supposed to have the tiny hole. The chip is opaque in the TEM, way too
thick, the thin area is translucent at 80KV so we can sort of see where we
are going.

We rigged up a special TEM holder so we could put the chip in the TEM, but
searching for the FIB hole, then the tiny hole inside that took a while.
Eventually I could see something that looked like a hole and it was about
100 nm across. He said that was OK, it was a test hole and should be about
100 nm.

Finding this 100 nm hole was no picnic and I anticipate finding and
measuring a 2 nm hole to be harder. He wants to be able to pop one in and
check the diameter and pop in another one.

Anyone with some bright ideas about how to help this guy? We brainstormed
things like FESEM, AFM, some kind of diffraction with lasers, don't know if
any of them will work, don't have any more ideas.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: dianavd-at-eye.usyd.edu.au
Date: Mon, 8 Aug 2005 20:09:12 -0500
Subject: [Microscopy] viaWWW: Sodium Azide and TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've seen it as a constituent of a TEM fixative, so it shouldn't be a
problem. But why bother?


Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
}
}
} Email: elke.buschbeck-at-uc.edu
} Name: Elke
}
} Organization: University of Cincinnati
}
} Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
}
} Question: Does anyone know if it is o.k. to use sodium azide with TEM
} preps? Does it wash out o.k. or will it leave a nasty deposit?
} Thanks
}


==============================Original Headers==============================
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From: ars-at-sem.com
Date: Tue, 9 Aug 2005 00:20:36 -0500
Subject: [Microscopy] RE: Looking for tiny holes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Any idea if there will be a single hole per sample or multiple holes? Will
you have any idea what the probable depth of the small hole will be (not
including the thinning they are apparently doing to the substrate)? The
hole's aspect ratio will have a lot to do with any analysis since a large
ratio (small diameter hole, long bore) can make alignment with any imaging
system a big problem.

This is probably a good time to jump in and see if they can't adjust their
experiment a little. Too often researchers jump before they can walk, and
assume that an existing method can provide whatever information they need,
without providing any compliance to existing techniques. A thinner
substrate would require less preliminary FIB thinning on their part and if
it were thin enough, could allow you to determine and measure the presence
of a hole even if the bore weren't perfectly aligned with the beam.

While they may be reluctant to discuss the work they are doing, details
aren't really needed. It may be in their best interest to understand the
hole formation process before extending it to such a thick substrate that
requires multiple operations. Unfortunately, it may be up to you to point
that out.


Allen R. Sampson, Owner
Advanced Research Systems
317 North 4th. Street
Saint Charles, Illinois 60174
phone (630) 513-7093 fax (630) 513-7092
email mailto:ars-at-sem.com web www.sem.com


On Monday, August 08, 2005 5:51 PM, jmkrupp-at-cats.ucsc.edu
[SMTP:jmkrupp-at-cats.ucsc.edu] wrote:
}
}
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
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------------------------------------------------------------------------
----
}
} Hi:
}
} A guy came into the lab today asking me to image and measure some holes
in
} a silicon nitride wafer.
}
} OK, I say, how big?
}
} 2 nm he says, some maybe up to 70 nm.
}
} That's pretty small, I said. How did you plan on doing it?
}
} Don't know, that's why I came to you, says he.
}
} Here is what he's got. A chip about 1 mm thick with an FIB section out of
} it that is like an inverted pyramid into the chip. The pyramid doesn't go
} all the way through, but there is a thin area at the bottom that is
} supposed to have the tiny hole. The chip is opaque in the TEM, way too
} thick, the thin area is translucent at 80KV so we can sort of see where
we
} are going.
}
} We rigged up a special TEM holder so we could put the chip in the TEM,
but
} searching for the FIB hole, then the tiny hole inside that took a while.
} Eventually I could see something that looked like a hole and it was about
} 100 nm across. He said that was OK, it was a test hole and should be
about
} 100 nm.
}
} Finding this 100 nm hole was no picnic and I anticipate finding and
} measuring a 2 nm hole to be harder. He wants to be able to pop one in and
} check the diameter and pop in another one.
}
} Anyone with some bright ideas about how to help this guy? We brainstormed
} things like FESEM, AFM, some kind of diffraction with lasers, don't know
if
} any of them will work, don't have any more ideas.
}
} Thanks
}
} Jon
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
} ==============================Original
Headers==============================
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} 15, 17 -- Subject: Looking for tiny holes
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==============================Original Headers==============================
7, 20 -- From ars-at-sem.com Tue Aug 9 00:20:36 2005
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From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Tue, 9 Aug 2005 02:09:16 -0500
Subject: [Microscopy] Energy dispersive spectroscopy fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't know your exact problem, but some names of people who have
worked with tissues and elemental analysis:

P. Echlin
H. Plattner
A. Somlyo
RA Steinbrecht and K. Zierold

There are many more - sorry for not mentioning these names.

best regards,
RR

-------------------------------
PD Dr.Reinhard Rachel
Universität Regensburg
Lehrstuhl für Mikrobiologie
Universitaetsstr. 31
D-93053 Regensburg
tel.: +49 941 943 4534
fax.: +49 941 943 1824



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From: luc-at-anaspec.co.za
Date: Tue, 9 Aug 2005 02:13:18 -0500
Subject: [Microscopy] I will be in Peru from the 11th Aug to 20 Aug.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be in Peru from the 11th Aug to 20 Aug.
I will have a delayed time acces to emails.
For urgent quotes and information please contact Rachel in Johannesburg.

==============================Original Headers==============================
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From: luc-at-anaspec.co.za
Date: Tue, 9 Aug 2005 02:16:20 -0500
Subject: [Microscopy] I will be in Peru from the 11th Aug to 20 Aug.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be in Peru from the 11th Aug to 20 Aug.
I will have a delayed time acces to emails.
For urgent quotes and information please contact Rachel in Johannesburg.

==============================Original Headers==============================
1, 14 -- From luc-at-anaspec.co.za Tue Aug 9 02:16:20 2005
1, 14 -- Received: from anaspec.co.za (mailix.bdse.net [196.14.233.10])
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1, 14 -- Mime-Version: 1.0
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1, 14 -- From: "Harmsen" {luc-at-anaspec.co.za}
1, 14 -- Reply-To: {luc-at-anaspec.co.za}
1, 14 -- To: microscopy-at-microscopy.com
1, 14 -- Subject: I will be in Peru from the 11th Aug to 20 Aug.
1, 14 -- X-Mailer: {SMTP32 v8.15}
1, 14 -- Precedence: bulk
==============================End of - Headers==============================




From: luc-at-anaspec.co.za
Date: Tue, 9 Aug 2005 02:19:32 -0500
Subject: [Microscopy] I will be in Peru from the 11th Aug to 20 Aug.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be in Peru from the 11th Aug to 20 Aug.
I will have a delayed time acces to emails.
For urgent quotes and information please contact Rachel in Johannesburg.

==============================Original Headers==============================
1, 14 -- From luc-at-anaspec.co.za Tue Aug 9 02:19:32 2005
1, 14 -- Received: from anaspec.co.za (mailix.bdse.net [196.14.233.10])
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1, 14 -- Mime-Version: 1.0
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1, 14 -- From: "Harmsen" {luc-at-anaspec.co.za}
1, 14 -- Reply-To: {luc-at-anaspec.co.za}
1, 14 -- To: microscopy-at-microscopy.com
1, 14 -- Subject: I will be in Peru from the 11th Aug to 20 Aug.
1, 14 -- X-Mailer: {SMTP32 v8.15}
1, 14 -- Precedence: bulk
==============================End of - Headers==============================




From: luc-at-anaspec.co.za
Date: Tue, 9 Aug 2005 02:22:37 -0500
Subject: [Microscopy] I will be in Peru from the 11th Aug to 20 Aug.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be in Peru from the 11th Aug to 20 Aug.
I will have a delayed time acces to emails.
For urgent quotes and information please contact Rachel in Johannesburg.

==============================Original Headers==============================
1, 14 -- From luc-at-anaspec.co.za Tue Aug 9 02:22:37 2005
1, 14 -- Received: from anaspec.co.za (mailix.bdse.net [196.14.233.10])
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1, 14 -- for {microscopy-at-microscopy.com} ; Tue, 9 Aug 2005 02:22:35 -0500
1, 14 -- Date: Tue, 9 Aug 2005 09:22:30 +0200
1, 14 -- Message-Id: {10508090922.AA2793505-at-anaspec.co.za}
1, 14 -- Mime-Version: 1.0
1, 14 -- Content-Type: text/plain; charset=us-ascii
1, 14 -- From: "Harmsen" {luc-at-anaspec.co.za}
1, 14 -- Reply-To: {luc-at-anaspec.co.za}
1, 14 -- To: microscopy-at-microscopy.com
1, 14 -- Subject: I will be in Peru from the 11th Aug to 20 Aug.
1, 14 -- X-Mailer: {SMTP32 v8.15}
1, 14 -- Precedence: bulk
==============================End of - Headers==============================




From: luc-at-anaspec.co.za
Date: Tue, 9 Aug 2005 02:26:01 -0500
Subject: [Microscopy] I will be in Peru from the 11th Aug to 20 Aug.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't care
Chris

----- Original Message -----
X-from: {luc-at-anaspec.co.za}
To: {cjeffree-at-staffmail.ed.ac.uk}
Sent: Tuesday, August 09, 2005 8:22 AM

I will be in Peru from the 11th Aug to 20 Aug.
I will have a delayed time acces to emails.
For urgent quotes and information please contact Rachel in Johannesburg.

==============================Original Headers==============================
1, 14 -- From luc-at-anaspec.co.za Tue Aug 9 02:26:01 2005
1, 14 -- Received: from anaspec.co.za (mailix.bdse.net [196.14.233.10])
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1, 14 -- for {microscopy-at-microscopy.com} ; Tue, 9 Aug 2005 02:25:59 -0500
1, 14 -- Date: Tue, 9 Aug 2005 09:25:56 +0200
1, 14 -- Message-Id: {10508090925.AA2793513-at-anaspec.co.za}
1, 14 -- Mime-Version: 1.0
1, 14 -- Content-Type: text/plain; charset=us-ascii
1, 14 -- From: "Harmsen" {luc-at-anaspec.co.za}
1, 14 -- Reply-To: {luc-at-anaspec.co.za}
1, 14 -- To: microscopy-at-microscopy.com
1, 14 -- Subject: I will be in Peru from the 11th Aug to 20 Aug.
1, 14 -- X-Mailer: {SMTP32 v8.15}
1, 14 -- Precedence: bulk
==============================End of - Headers==============================




From: luc-at-anaspec.co.za
Date: Tue, 9 Aug 2005 02:28:52 -0500
Subject: [Microscopy] I will be in Peru from the 11th Aug to 20 Aug.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be in Peru from the 11th Aug to 20 Aug.
I will have a delayed time acces to emails.
For urgent quotes and information please contact Rachel in Johannesburg.

==============================Original Headers==============================
1, 14 -- From luc-at-anaspec.co.za Tue Aug 9 02:28:51 2005
1, 14 -- Received: from anaspec.co.za (mailix.bdse.net [196.14.233.10])
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1, 14 -- for {microscopy-at-microscopy.com} ; Tue, 9 Aug 2005 02:28:50 -0500
1, 14 -- Date: Tue, 9 Aug 2005 09:28:47 +0200
1, 14 -- Message-Id: {10508090928.AA21798955-at-anaspec.co.za}
1, 14 -- Mime-Version: 1.0
1, 14 -- Content-Type: text/plain; charset=us-ascii
1, 14 -- From: "Harmsen" {luc-at-anaspec.co.za}
1, 14 -- Reply-To: {luc-at-anaspec.co.za}
1, 14 -- To: microscopy-at-microscopy.com
1, 14 -- Subject: I will be in Peru from the 11th Aug to 20 Aug.
1, 14 -- X-Mailer: {SMTP32 v8.15}
1, 14 -- Precedence: bulk
==============================End of - Headers==============================




From: pwje-at-sympatico.ca
Date: Tue, 9 Aug 2005 08:05:18 -0500
Subject: [Microscopy] Re: AskAMicroscopist: schematics for an International Scientific

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi George
Did you ever get the schematics for the ISI 100B that you were looking for?
Cheers
Peter Earl
Toronto


==============================Original Headers==============================
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2, 26 -- References: {200507180006.j6I066ku023229-at-ns.microscopy.com}
2, 26 -- Subject: Re: [Microscopy] AskAMicroscopist: schematics for an International Scientific
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From: randerson20-at-tampabay.rr.com
Date: Tue, 9 Aug 2005 08:43:17 -0500
Subject: [Microscopy] Re: Microscopy Technician position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr.Henderson,

I noticed with interest your recent employment note on the Microscopy
listserver and hope that you are having good success.

Our publication, Microscopy Today, is mailed six times per year to
nearly 15,000 microscopists -- each of whom has either specifically
requested the publication or were automatically subscribed as an MSA
member benefit. Our next issue closes on August 15th and will be mailed
on or about September 15th. You may consider placing your employment ad
with us.

We offer employment advertisements at the reduced rate of $450 for a
quarter-page (3 5/8 in. W X 5 in H) insertion. Larger (3 5/8"
W)employment ads can be purchased for an additional $90 per inch over 5
inches high.

Should you be interested, normal procedure is for you to supply your
text by email , or fax the copy to us at (727) 507-7102. We will
typeset, add a border, etc., and email back a PDF draft copy of the
advertisement with a quote at no obligation to you. After your price
approval, proof, changes, corrections, and billing instructions, are
received, we will publish. You may include a logo if you wish.

Kindly advise rather soon should you be interested in this proposal as
the timing for this issue is becoming critical.

Regards,

Ron Anderson, Editor
Microscopy Today


schenderson-at-vcu.edu wrote:

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From: David.Patton-at-uwe.ac.uk
Date: Tue, 9 Aug 2005 09:21:45 -0500
Subject: [Microscopy] viaWWW: Sodium Azide and TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Why use sodium azide?

We are planning to use sodium azide for the first time as we wish to
store SEM samples for 3-4 months (rare samples available now for a
student project in November). I have found that very occasionally
something can grow in sodium cacodylate buffer. I think there are some
fungi that like it :)


Dave

-----Original Message-----
X-from: dianavd-at-eye.usyd.edu.au [mailto:dianavd-at-eye.usyd.edu.au]
Sent: 09 August 2005 02:11
To: David Patton

I've seen it as a constituent of a TEM fixative, so it shouldn't be a
problem. But why bother?


Diana van Driel
Dept Ophthalmology
Sydney University
GPO Box 4337
Sydney, NSW
AUSTRALIA 2001

Phone 61 2 93827278
Mobile 0423 151614
FAX 61 2 93827318
}
}
} Email: elke.buschbeck-at-uc.edu
} Name: Elke
}
} Organization: University of Cincinnati
}
} Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
}
} Question: Does anyone know if it is o.k. to use sodium azide with TEM
} preps? Does it wash out o.k. or will it leave a nasty deposit?
} Thanks
}


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19, 32 -- From: David Patton {David.Patton-at-uwe.ac.uk}
19, 32 -- Subject: RE: [Microscopy] viaWWW: Sodium Azide and TEM
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From: randerson20-at-tampabay.rr.com
Date: Tue, 9 Aug 2005 09:34:23 -0500
Subject: [Microscopy] Sorry about the broadcast email from MT!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

1. Is my face red! Only yesterday I read that with the new listserver
setup that hitting reply--intending to send mail to the person who
originated the initial email only--in fact sends the reply to the whole
listserver! Please disregard the email to Henderson. I'll try my best
not to make that mistake again! I'm sorry.

2. To the folks who sent me nasty, nearly obscene diatribes castigating
me for this simple mistake: please try and get a grip on life. Perhaps a
nice nap will help.

Ron Anderson
Microscopy Today




==============================Original Headers==============================
6, 20 -- From randerson20-at-tampabay.rr.com Tue Aug 9 09:34:23 2005
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6, 20 -- Date: Tue, 09 Aug 2005 10:34:13 -0400
6, 20 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com}
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From: hoffpajo-at-yahoo.com
Date: Tue, 9 Aug 2005 10:19:16 -0500
Subject: [Microscopy] Re: Sorry about the broadcast email from MT!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

one can only wonder if these same people have time to
do anything else beside criticize others.
i have been on the receiving end of these diatribes.
and yes people do need to get a life.
john
ps and yes i meant to send this to the whole
listserver

--- randerson20-at-tampabay.rr.com wrote:

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} 1. Is my face red! Only yesterday I read that with
} the new listserver
} setup that hitting reply--intending to send mail to
} the person who
} originated the initial email only--in fact sends the
} reply to the whole
} listserver! Please disregard the email to
} Henderson. I'll try my best
} not to make that mistake again! I'm sorry.
}
} 2. To the folks who sent me nasty, nearly obscene
} diatribes castigating
} me for this simple mistake: please try and get a
} grip on life. Perhaps a
} nice nap will help.
}
} Ron Anderson
} Microscopy Today
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}
} ==============================Original
} Headers==============================
} 6, 20 -- From randerson20-at-tampabay.rr.com Tue Aug 9
} 09:34:23 2005
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} {randerson20-at-tampabay.rr.com}
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} from MT!
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__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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From: hoffpajo-at-yahoo.com
Date: Tue, 9 Aug 2005 10:20:17 -0500
Subject: [Microscopy] Re: Extending New Homes with options.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i didn't know the listserver was offering home loans.

--- ypuizaluzec-at-microscopy.com wrote:

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} Hello
}
} Homeowner
}
} You have been pre-approved for a $402,974 Home Loan
} at a 4.53% Fixed Rate.
} This offer is being extended to you unconditionally
} and your credit is in no way a factor.
}
} To take Advantage of this Limited Time opportunity
}
} All we ask is that you visit our Website and
} complete
} The 1 minute post Approval Form
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} http://mrYC3sFjAhXM0.loanvoice.com/?name=rnnn
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} Have a Good Day,
}
} Nelle Kendall
}
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} 8, 13 -- From ypuizaluzec-at-microscopy.com Tue Aug 9
} 08:07:44 2005
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From: kestel-at-anl.gov
Date: Tue, 9 Aug 2005 10:24:48 -0500
Subject: [Microscopy] Looking for tiny holes

Contents Retrieved from Microscopy Listserver Archives
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On 8/9/05 12:24 AM, "ars-at-sem.com" {ars-at-sem.com} wrote:

}
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}
} Any idea if there will be a single hole per sample or multiple holes? Will
} you have any idea what the probable depth of the small hole will be (not
} including the thinning they are apparently doing to the substrate)? The
} hole's aspect ratio will have a lot to do with any analysis since a large
} ratio (small diameter hole, long bore) can make alignment with any imaging
} system a big problem.
}
} This is probably a good time to jump in and see if they can't adjust their
} experiment a little. Too often researchers jump before they can walk, and
} assume that an existing method can provide whatever information they need,
} without providing any compliance to existing techniques. A thinner
} substrate would require less preliminary FIB thinning on their part and if
} it were thin enough, could allow you to determine and measure the presence
} of a hole even if the bore weren't perfectly aligned with the beam.
}
} While they may be reluctant to discuss the work they are doing, details
} aren't really needed. It may be in their best interest to understand the
} hole formation process before extending it to such a thick substrate that
} requires multiple operations. Unfortunately, it may be up to you to point
} that out.
}
}
} Allen R. Sampson, Owner
} Advanced Research Systems
} 317 North 4th. Street
} Saint Charles, Illinois 60174
} phone (630) 513-7093 fax (630) 513-7092
} email mailto:ars-at-sem.com web www.sem.com
}
}
} On Monday, August 08, 2005 5:51 PM, jmkrupp-at-cats.ucsc.edu
} [SMTP:jmkrupp-at-cats.ucsc.edu] wrote:
} }
} }
} }
} }
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} }
} } Hi:
} }
} } A guy came into the lab today asking me to image and measure some holes
} in
} } a silicon nitride wafer.
} }
} } OK, I say, how big?
} }
} } 2 nm he says, some maybe up to 70 nm.
} }
} } That's pretty small, I said. How did you plan on doing it?
} }
} } Don't know, that's why I came to you, says he.
} }
} } Here is what he's got. A chip about 1 mm thick with an FIB section out of
} } it that is like an inverted pyramid into the chip. The pyramid doesn't go
} } all the way through, but there is a thin area at the bottom that is
} } supposed to have the tiny hole. The chip is opaque in the TEM, way too
} } thick, the thin area is translucent at 80KV so we can sort of see where
} we
} } are going.
} }
} } We rigged up a special TEM holder so we could put the chip in the TEM,
} but
} } searching for the FIB hole, then the tiny hole inside that took a while.
} } Eventually I could see something that looked like a hole and it was about
} } 100 nm across. He said that was OK, it was a test hole and should be
} about
} } 100 nm.
} }
} } Finding this 100 nm hole was no picnic and I anticipate finding and
} } measuring a 2 nm hole to be harder. He wants to be able to pop one in and
} } check the diameter and pop in another one.
} }
} } Anyone with some bright ideas about how to help this guy? We brainstormed
} } things like FESEM, AFM, some kind of diffraction with lasers, don't know
} if
} } any of them will work, don't have any more ideas.
} }
} } Thanks
} }
} } Jon
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
} }
} }
} }
} } ==============================Original
} Headers==============================
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} } 15, 17 -- From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
} } 15, 17 -- Subject: Looking for tiny holes
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} 7, 20 -- From ars-at-sem.com Tue Aug 9 00:20:36 2005
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} 7, 20 -- From: "Allen R. Sampson" {ars-at-sem.com}
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} 7, 20 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
} 7, 20 -- Subject: RE: [Microscopy] Looking for tiny holes
} 7, 20 -- Date: Tue, 9 Aug 2005 00:20:29 -0500
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}
}
} The thickness at a hole depends upon whether a precipitate fell out, which
leaves a thicker region around a hole. If a hole is formed by electropolishing
an annealled sample of a pure metal, or even some alloys, the area around the
hole may be very thin. 20 to 50 nanometers might be typical thicknesses
achieved.
Bernie Kestel
Argonne National Lab E-mail: kestel-at-anl.gov


==============================Original Headers==============================
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From: lcgould-at-med.cornell.edu
Date: Tue, 9 Aug 2005 10:26:11 -0500
Subject: [Microscopy] Re: Microscopy Technician position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sandy,
I saw that yesterday. I guess things are working out for Scott, if
his lab is growing. That's good.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

==============================Original Headers==============================
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1, 23 -- Subject: [Microscopy] Re: Microscopy Technician position available
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From: zaluzec-at-microscopy.com
Date: Tue, 9 Aug 2005 11:46:35 -0500
Subject: [Microscopy] Administrivia: SPAM and Replies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues;

I believe I have plugged the loop-hole that allowed the Housing
for Sale Email to get through the system earlier today. It was
a unique situation to say the least, but then again they are all
getting this way.

At the same time since I was editing the code I have also changed the
Listserver Software so that a reply should nolonger go automatically
to the Listserver but to the sender.

Nevertheless I caution you to please always look at what your sending.
It only takes a moment.

If you wish to send a copy of your "reply" to a message to the Listserver
you will now have to explicitly add the Listserver Email
address (microscopy-at-microscopy.com ) to your recepients list

Nestor
Your Friendly Neighborhood SysOp




--

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From: JOHN.WHEATLEY-at-asu.edu
Date: Tue, 9 Aug 2005 12:48:49 -0500
Subject: [Microscopy] RE: viaWWW: Sodium Azide and TEM

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Sodium azide should be used very carefully. It has been used as a
biological preservative for a long time. In 1968 I was working in a
CDC lab in Phoenix (Yes, there was a CDC branch there at that time)
where we used sodium azide to preserve hepatitis antigens. One of
the technicians was using an aspiration tube attached to a pipette to
transfer sodium azide solution to an antigen preparation. She
coughed and sucked the solution into her lungs. She was on the floor
gasping for breath within 15 seconds. There was an MD sitting in
his office next to the technician's lab. He heard her fall to the
floor. He new what she was doing and determined what had happened.
He had what he called a "universal antitdote" in his office. He
administered the antidote and gave her mouth-to-mouth resuscitation
and she survived.

I know (I hope!!) people don't use aspiration tubes today but this
incident sure brought home to me how how quickly sodium azide works.
You may want to look at the following CDC URL:
http://www.bt.cdc.gov/agent/sodiumazide/basics/facts.asp
Although this article states that there is no specific antidote for
sodium azide poisoning, in the incident I related above, an antidote
was given. I don't remember what it was.




} ----------
} From: dianavd-at-eye.usyd.edu.au
} Reply To: microscopy-at-microscopy.com
} Sent: Monday, August 8, 2005 6:11 PM
} To: John Wheatley
} Subject: [Microscopy] viaWWW: Sodium Azide and TEM
}
}
}
}
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} I've seen it as a constituent of a TEM fixative, so it shouldn't be a
} problem. But why bother?
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University
} GPO Box 4337
} Sydney, NSW
} AUSTRALIA 2001
}
} Phone 61 2 93827278
} Mobile 0423 151614
} FAX 61 2 93827318
} }
} }
} } Email: elke.buschbeck-at-uc.edu
} } Name: Elke
} }
} } Organization: University of Cincinnati
} }
} } Title-Subject: [Filtered] MListserver:Sodium Azide and TEM
} }
} } Question: Does anyone know if it is o.k. to use sodium azide with TEM
} } preps? Does it wash out o.k. or will it leave a nasty deposit?
} } Thanks
} }
}
}
} ==============================Original Headers==============================
} 5, 20 -- From dianavd-at-eye.usyd.edu.au Mon Aug 8 20:09:11 2005
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} 5, 20 -- Subject: Re: [Microscopy] Re: viaWWW: Sodium Azide and TEM
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==============================Original Headers==============================
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7, 26 -- Subject: RE: [Microscopy] viaWWW: Sodium Azide and TEM
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12, 15 -- From: JOHN.WHEATLEY-at-asu.edu (by way of Nestor J. Zaluzec)
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From: mcmorran-at-physics.arizona.edu
Date: Tue, 9 Aug 2005 14:30:30 -0500
Subject: [Microscopy] Re: Looking for tiny holes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We use a standard SEM to work with nanometer-scale features (~50 nm) in
silicon
nitride. You might consider using electron diffraction. If using a 10
keV beam,
(wavelength of .12 angstroms), a circular aperture with a diameter of 2
nm would
create a circular diffraction pattern (described by an Airy disk). With these
parameters, the radius of the first zero is given by R = 0.007*(distance from
the aperture).

Dunno much about TEM and standard detectors used, but if you have an electron
detector with a spatial resolution of 10 microns placed 1 cm below the sample,
you would be able to resolve the central diffraction order (thereby measuring
the hole diameter). To do this, you'd turn off the scanning mechanism for the
microscope, and you'd want to focus quite a bit beyond the hole itself (to
ensure the electron wavefronts extend across the diameter of the hole.

Anyway, I'm sure there are more straightforward solutions, but this is just an
idea. We do similar stuff all the time.

--
Ben McMorran
Research Assistant, Atom Optics Group
Department of Physics
University of Arizona
1118 E 4th St
Tucson, AZ
USA

ph. 520-621-2688

Quoting jmkrupp-at-cats.ucsc.edu:

} ----------------------------------------------------------------------------
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}
} Hi:
}
} A guy came into the lab today asking me to image and measure some holes in
} a silicon nitride wafer.
}
} OK, I say, how big?
}
} 2 nm he says, some maybe up to 70 nm.
}
} That's pretty small, I said. How did you plan on doing it?
}
} Don't know, that's why I came to you, says he.
}
} Here is what he's got. A chip about 1 mm thick with an FIB section out of
} it that is like an inverted pyramid into the chip. The pyramid doesn't go
} all the way through, but there is a thin area at the bottom that is
} supposed to have the tiny hole. The chip is opaque in the TEM, way too
} thick, the thin area is translucent at 80KV so we can sort of see where we
} are going.
}
} We rigged up a special TEM holder so we could put the chip in the TEM, but
} searching for the FIB hole, then the tiny hole inside that took a while.
} Eventually I could see something that looked like a hole and it was about
} 100 nm across. He said that was OK, it was a test hole and should be about
} 100 nm.
}
} Finding this 100 nm hole was no picnic and I anticipate finding and
} measuring a 2 nm hole to be harder. He wants to be able to pop one in and
} check the diameter and pop in another one.
}
} Anyone with some bright ideas about how to help this guy? We brainstormed
} things like FESEM, AFM, some kind of diffraction with lasers, don't know if
} any of them will work, don't have any more ideas.
}
} Thanks
}
} Jon
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
} ==============================Original Headers==============================
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==============================Original Headers==============================
9, 27 -- From mcmorran-at-physics.arizona.edu Tue Aug 9 14:30:30 2005
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From: walck-at-southbaytech.com
Date: Tue, 9 Aug 2005 22:25:49 -0500
Subject: [Microscopy] viaWWW: Field Ion Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I didn't see that you got a response on this, so I thought that I would
do so.


I built a FIM/IAP for my dissertation work back in the early to mid
80's. Mine was made using UHV stainless steel components. I was
familiar with most of the people in the field at that time and didn't
know anyone that was still putting together glass systems. I think that
it has been a long time since people have been using glass systems for
FIM. When they were, mostly they had their systems custom made by their
in-house glass blowers.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: tttan-at-simtech.a-star.edu.sg [mailto:tttan-at-simtech.a-star.edu.sg]
Sent: Tuesday, August 02, 2005 11:05 AM
To: Walck-at-SouthBayTech.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (tttan-at-simtech.a-star.edu.sg) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
August 1, 2005 at 20:24:16
------------------------------------------------------------------------
---

Email: tttan-at-simtech.a-star.edu.sg
Name: TT Tan

Organization: Singapore Inst. of Manuf. Tech.

Title-Subject: [Filtered] Field Ion Microscope

Question: Hi all,

I would like to know from who can I buy the glassware to do field ion
microscopy.

I am looking for a quartz ware that can do the job. Preferably one
that allows me to fit onto a NW40 joint.

Thanks in advance.

------------------------------------------------------------------------
---

==============================Original
Headers==============================
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21, 26 -- From walck-at-southbaytech.com Tue Aug 9 22:25:48 2005
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From: montiorm-at-ucmail.uc.edu
Date: Tue, 9 Aug 2005 22:26:33 -0500
Subject: [Microscopy] viaWWW: TCS 4D available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (montiorm-at-ucmail.uc.edu) from http://microscopy.com/MLFormMail.html on Thursday, July 28, 2005 at 12:57:07
---------------------------------------------------------------------------

Email: montiorm-at-ucmail.uc.edu
Name: Richard Montione

Organization: University of Cincinnati

Title-Subject: [Filtered] TCS 4D available

Question: RE: TCS 4D



To anyone interested,



I have a Leica TCS 4D confocal system originally installed in 1994. It has a Kr/Ar laser and a water cooled UV laser, with a Leitz DMRBE upright microscope and a number of lenses. It has the usual problems associated with age but was still functional when it was disassembled for storage.



If anyone is interested and would like more information please contact me at montiorm-at-uc.edu





Richard Montione

Electron Microscopy Facility

Department of Pathology

University of Cincinnati




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==============================Original Headers==============================
20, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:26:33 2005
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==============================End of - Headers==============================




From: msimms-at-tracelabs.com
Date: Tue, 9 Aug 2005 22:29:45 -0500
Subject: [Microscopy] viaWWW: Measurement of ink depth/contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (msimms-at-tracelabs.com) from http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, July 29, 2005 at 10:06:10
---------------------------------------------------------------------------

Email: msimms-at-tracelabs.com
Name: Michael Simms

Organization: Trace Laboratories - Central

Title-Subject: [Filtered] Measurement of ink depth/contrast

Question: Hello Gentlemen,
I work for an independent testing organization, Trace Laboratories - Central.
We have been asked to have printing ink measured for depth and contrast. Laser printing is done on the insulation sleeve of a cable.
per Sikorsky specification SS7333, Paragraph 4.6.5
This asks for measuring to an accuracy of 0.0001 inch with a contour projector or calibrated microscope or Zygo measuring device. The contrast measurement is suggested to be made with a Spectrum Technology CMS^2 Contrast Measuring System. I would need evidence of either an accredited laboratory or calibration to pass on to the customer.

Is this something that someone associated with this resource might be able to quote?

Regards,
Mike

Mike Simms
Chemist
Trace Laboratories - Central
1150 W. Euclid Ave.
Palatine, IL 60067

phone 847-934-5300
fax 847-934-4600
www.tracelabs.com



---------------------------------------------------------------------------

==============================Original Headers==============================
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From: stefan.diller-at-t-online.de
Date: Tue, 9 Aug 2005 22:31:03 -0500
Subject: [Microscopy] viaWWW: Reichert Histostat 8035 Paraffin Embedder manual needed

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (stefan.diller-at-t-online.de) from http://microscopy.com/MLFormMail.html on Saturday, July 30, 2005 at 13:43:48
---------------------------------------------------------------------------

Email: stefan.diller-at-t-online.de
Name: Stefan Diller

Title-Subject: [Filtered] Reichert Histostat 8035 Paraffin Embedder manual needed

Question: Dear members,
I am looking urgently for a user manual and if possible a service manual or the electronic layout for the Reichert Histostat Paraffin Embedder Modell 8035.

Best regards,
Stefan Diller


---------------------------------------------------------------------------

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==============================End of - Headers==============================




From: ken.blight-at-cancer.org.uk
Date: Tue, 9 Aug 2005 22:32:28 -0500
Subject: [Microscopy] viaWWW: Drosophila eye ultrathin cryosections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ken.blight-at-cancer.org.uk) from http://microscopy.com/MLFormMail.html on Wednesday, August 3, 2005 at 10:40:02
---------------------------------------------------------------------------

Email: ken.blight-at-cancer.org.uk
Name: Ken Blight

Organization: cancer research uk

Title-Subject: [Filtered] Drosophila eye ultrathin cryosections

Question: I have processed Drosophila eyes for immunolabelling (EM) using the Tokuyasu technique and the results are, to say the least, very disappointing. The eyes floated in the fixative which didn't help matters. The problem looks to be spacial when I compare the results to standard resin TEM preparation. Maybe there is some problem with buffers, fixation or section expansion. Does anyone have experience of these tough little beasties?

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From: cornheadorama-at-hotmail.com
Date: Tue, 9 Aug 2005 22:33:19 -0500
Subject: [Microscopy] viaWWW: Cambridge S250

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 3, 2005 at 18:07:30
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver: Re: Cambridge S250

Question: Hi,

I'm not too familiar with these despite the fact I have two of them gathering dust. But I have heard them disfavorably compared to the S200. Mine may be site-rigged for handling semi wafers (and there's a chance both came from the same facility, at different times... a hunch) but in mine the stage uses little more substantial than pvc tubing to transmit the z-axis knob to the stage: horrible hysteresis.

I suspect these and related scopes were enormously popular, and I know for a fact this 'scope has die-hard fans.

LaB6 is typical but it wouldn't surprise me if there was an active aftermarket upgrade to FE: indeed one of mine may have one. On the other hand, you may have missed the bulk of the aftermarket boat by about 5 years: lots of parts for old scopes falling off of price lists.... but sometimes onto "clearance" lists :)

But I'd say even if your own scope leaves a lot to be desired, trading it for another old scope like this? I'd say deosn't quite make sense unless you have a special requirement. Ultimately, it just may not be worth the shipping: which of course is dicey unless there's at least a few hours teardown and re-assembly. Better the devil you know.

I hope more people respond, I've been back and forth as to whether to try to get one or both of mine working for about 6 years now :)

-Jeff


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From: cornheadorama-at-hotmail.com
Date: Tue, 9 Aug 2005 22:34:38 -0500
Subject: [Microscopy] viaWWW: Re: Osmium Vapors

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 3, 2005 at 18:41:52
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Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: self

Title-Subject: [Filtered] MListserver:b Re: Osmium Vapors

Question: I don't have experience with osmium compounds, and my suggestion may not be very practical (read: expensive) unless you have piles of vacuum fittings lying around like I do, but on occasion when wanting to "absolutely" seal something up I've used a KF50 stainless nipple (or bellows, sometimes)of appropriate length, a pair of stainless blanks, clamps, o-rings etc. so that the item could be sealed "vacuum tight". Of course it's all relative but I wouldn't think much vapour will diffuse though the ~5mm of highly compressed Viton. The clamps are a bit clumsy to put on but very quick to come off: I'm not sure you can get a quick-release clamp in KF50 but that would make it almost one-handed. You could probably save money and thru-gassing by having a single-ended arrangment, ie: a pipe section with a KF50 flange welded on one end and a cap welded on the other. Some (if not most) of the hi-vac houses weld their standard catalog items on-site, and some don't charge much extra (~%20 premium) for custom items from stock parts.

I think the idea of absorbants/reactants holds a lot of potential. Activated charcoal comes immediately to mind.
Is it so reactive as to present a fire hazard if quickly adsorbed onto the huge surface area?

-Jeff

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From: omelon-at-geology.utoronto.ca
Date: Tue, 9 Aug 2005 22:35:31 -0500
Subject: [Microscopy] viaWWW: further hardening of LR White

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (omelon-at-geology.utoronto.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, August 4, 2005 at 16:57:53
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Email: omelon-at-geology.utoronto.ca
Name: Christopher R. Omelon

Organization: University of Toronto

Title-Subject: [Filtered] further hardening of LR White

Question: I have tried embedding large samples (~ 1cm3) of porous rock material in a variety of resins and find that LR White (Hard) provides the best infilling due to its low viscosity. That being said, the blocks are not as hard as when using epoxide resins such as EMBED 812. I am cold-curing (i.e. with accelerator) at both room temperature and at 4?C with mixed results, but even in the best case the blocks are not as hard as I would expect. My question: can I further harden the blocks by now placing these samples in the oven at 60?C? My reasoning is that resin within the block (i.e. beneath the surface and therefore not exposed to oxygen) will proceed with polymerization at a faster rate than if I left the blocks at room temperature. Or is the reaction now at completion? Thanks

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From: Stacey.Andringa-at-uc.edu
Date: Tue, 9 Aug 2005 22:37:06 -0500
Subject: [Microscopy] viaWWW: Kodak Dektol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 9, 2005 at 12:35:33
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Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: We have bags of Kodak Dektol. Does anyone know if I can use this to develop Kodak Em film 4489? At what dilution, for how long?
Thanks for any help.

Stacey Andringa

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From: Colin.Veitch-at-csiro.au
Date: Wed, 10 Aug 2005 01:59:31 -0500
Subject: [Microscopy] Hitachi S4100 screens and ergonomics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

We have just had an ergonomist look at our Hitachi S4100 set up as the
primary user is beginning to have some problems which could get very
nasty as time goes on.

Has anyone out there had similar issues? And if so, what issues were
felt to be critical and what solutions were offered?

One of the biggest issues (according to the ergonomist) is the position
of the screens. They are CRT's embedded in a console and as such can't
be moved. Has anyone tried to take the video signals from one of these
systems (or similar) and put them onto a LCD monitor? If so, how was it
done and how successful was it? (these screens are also beginning to
lose their intensity which isn't helping!)

Another issue is the arrangement of the console and column. The
ergonomist feels that they would be better at 90 degrees to each other
but the cable length between the column and console prevents that. Has
anyone tried to lengthen these cables (some go to the HV tank)?

Any help or information would be appreciated.

Cheers

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
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From: Gretchen.Ziegler-at-leica-microsystems.com
Date: Wed, 10 Aug 2005 06:12:16 -0500
Subject: [Microscopy] Re: AskAMicroscopist: an affordable student microscope

Contents Retrieved from Microscopy Listserver Archives
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Since I'm the Manager for the Educational Division I have to put my 2 cents
in.....

NEVER buy a microscope just because it is affordable. I always suggest
getting in a local dealer who can
provide you with a demonstration AND be there after the sale in case you
need service.

There are many "off brand" microscopes out there that are a good way to
through away money. I have been
in many labs where whole sets of microscopes are on the shell because one
or two starting smoking and the
lab manager was afraid to use them. I have also tried servicing some of
these off brands and the materials
used and the machining makes them good ship achors :-)

For your own best interest, call a local dealer and get a demonstration -
it's also good to ask for references from
people who have used the microscope you are looking to buy. Remember the
saying "you get what you pay for".
Bad quality microscopes will prevent you from being able show the students
what you are trying to teach.

If you have any questions, please do not hesitate to contact me.

Gretchen


Gretchen Ziegler, Sales Manager, Educational Division
Leica Microsystems, Inc.
P.O. Box 151 - Ocean Grove, NJ 07756
Phone: 732-897-9506 - Fax: 847-236-3013
Voicemail: 1-800-248-0665 ext. 5131



ballardmark-at-gmail
.com To: Gretchen.Ziegler-at-leica-microsystems.com
cc:
07/28/2005 09:08 Subject: [Microscopy] AskAMicroscopist: an affordable student microscope
PM
Please respond to
microscopy









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Email: ballardmark-at-gmail.com
Name: Marcello

Organization: None

Education: 9-12th Grade High School

Location: Orlando, Florida

Question: Hi to all,

i am trying to buy the most afforable microscopy, but that will be
able to help me with biology and chemistry.

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From: jrobson-at-rdg.boehringer-ingelheim.com
Date: Wed, 10 Aug 2005 07:32:07 -0500
Subject: [Microscopy] RE: Hitachi S4100 screens and ergonomics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Colin,

We have an Hitachi S-4000 which I believe is very similar to the S-4100. We
have encountered both of this issues and have addressed them as follows:

We attempted to turn the column cabinet in order to enable users better
access to the manual stage controls. As you observed the cable length is
insufficient to accomplish more than 20 -30 degree of rotation. We also
determined that turning the column introduced a new problem...it became
difficult to introduce a sample into the SEC port (which is our primary
sample entry port). Our solution was to purchase a motorized stage. While
this was a costly upgrade, it was money well spent. The 5 axis stage we
purchased from a company called E.Fjeld(http://www.efjeld.com/P_5500.htm)has
performed much better that anticipated. As expected wear and tear on the
user is dramatically improved! In addition the stage motion is much more
precise and backlash is nonexistent.

On the S-4000 there was a simple solution to adding a high resolution CRT
type monitor. A BNC port accessing the display crt already existed. If you
open the back lower console panel you should see two BNC ports in the upper
middle region (ours are unlabelled). We connected a cable from the left
port to the B & W monitor. If you have schematics for the system you should
be able to determine if this port exist on your system. Unfortunately I
don't know much about LCD monitors and their cabling options so this could
be an issue when selecting a monitor. However, we have been satisfied with
the B & W CRT. Also, we haven't noticed any adverse field effects while the
CRT monitor is in use.

While not addressed in your post a third area relating to ergonomics was
addressed by elimination of the Polaroid camera system. We purchased a
passive acquisition system from PCI
Quartz(http://www.qrtz.com/acquisition.html) which allows us to capture
digital images.

While the upgrades were costly they have improved the user interface,
increased productivity, and added to the useful lifetime of the instrument.
The system is 16+ years old and continues to produce quality results in a
digital environment. I'm not sure how much, if any, of this information is
helpful or relevant? However, if you have any question please feel free to
contact me directly. Good luck, jr.

Disclaimer: These are my opinions only and do not reflect those of my
employer. While I am a satisfied customer I have no financial ties to any of
these manufacturers. All of the upgrades mentioned are available from
multiple vendors and it is suggest that you investigate all options prior to
making any purchases for your system.


John A. Robson
Boehringer Ingelheim Pharmaceuticals, Inc.
PO Box 368
900 Ridgebury Rd
Ridgefield, CT 06877

Phone (203)798-5640
Fax (203)798-5698
e-mail jrobson-at-RDG.boehringer-ingelheim.com


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From: TindallR-at-missouri.edu
Date: Wed, 10 Aug 2005 08:30:28 -0500
Subject: [Microscopy] viaWWW: Kodak Dektol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

That's an easy one.....no! Dektol is for developing photo paper, not
film. Take it from someone who did this by mistake once and got
negatives with grain the size of gravel and films so thick you could use
them to view eclipses. It just might be possible with enough testing
and fooling to get negatives you could actually see through, but the
quality would be pretty awful anyway.

If you don't make prints anymore, donate your Dektol to your local
photography classes.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu





-----Original Message-----
X-from: Stacey.Andringa-at-uc.edu [mailto:Stacey.Andringa-at-uc.edu]
Sent: Tuesday, August 09, 2005 10:40 PM
To: Tindall, Randy D.

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (Stacey.Andringa-at-uc.edu) from
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12:35:33
------------------------------------------------------------------------
---

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: We have bags of Kodak Dektol. Does anyone know if I can use
this to develop Kodak Em film 4489? At what dilution, for how long?
Thanks for any help.

Stacey Andringa

------------------------------------------------------------------------
---

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From: lfox1-at-lumc.edu
Date: Wed, 10 Aug 2005 08:30:38 -0500
Subject: [Microscopy] TEM - liposome NTA bridge prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,
Can anyone advise this new user to our lab? His prep/questions are
below. I think that he needs negative staining. How best to prep this
sample?/fixation/washing out the sucrose?? and what type of filmed
grids are being used these days??Formvar/Pioloform??other??

-----------------------

Basically what I have is a 100nm virus particle bound to a 100nm
liposome molecule by a Nickel NTA "bridge". In short, I would like a
picture of this complex. So, If I had this complex (usually in
~10-20%sucrose solution), what would I do from there as far a
preparation?

----------------------
Thanks,
Linda

Linda M. Fox
Loyola University
Stritch School of Medicine
Core Imaging Facility
2160 S. First Ave.
Maywood, Il 60153
Bld. 102 Room 0617
1-708-216-3395
lfox1-at-lumc.edu


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From: peoshel-at-wisc.edu
Date: Wed, 10 Aug 2005 09:16:44 -0500
Subject: [Microscopy] Re: viaWWW: Energy dispersive spectroscopy fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bill,

Ron has been trying to get me to rejoin MT, so ...
This would make an excellent article, would you be willing to write
it up for MT?
Note, we'd also like articles on cryoTEM and tomography.

Phil

} On Aug 5, 2005, at 5:36 PM, sdmegs09-at-yahoo.com wrote:
}
} } Question: I am trying to fix plant material for EDS in the TEM and I
} } cannot find literature stating the fixation techniques. Is fixation
} } different for EDS?
} }
} Dear Meghan,
} The preparation methods for any sample on which you are doing EDS or
} other analytic techniques must take into account the elements you are
} trying to analyze, so, for example, if you are looking for
} water-soluble elements like Na or Cl ion, do not rinse the tissue in
} solvents that will remove them. Note, however, that if you are looking
} for Cl bound in organic compounds, such as polychlorinated biphenyls,
} washing out the ion will enable you to distinguish the organochlorine.
} For accurate quantitation, it is a good idea, if possible, to examine
} the material untreated, except for freezing and perhaps sectioning,
} then dehydrate by lyophylization in the scope and redo the analysis.
} This will give accurate values for volatile elements and you can
} improve the quantitation by taking the wet/dry ratios from elements
} that are not affected by the drying process. Another consideration,
} for the case that the elements of interest are not affected by your
} preparation process, is not to use a stain that interferes with lines
} from those elements. Pb and S are notorious for this, and one case I
} ran into was trying to analyze Pt and Ir in a specimen that was treated
} with Os and given to me. Since your specimens may not be suitable for
} observation in the TEM when not prepared in some way, and since you may
} not have access to cryopreparation methods, you need to keep in mind
} the general principles that, whatever the preparation steps, they
} cannot affect the concentration of the elements of interest, and cannot
} interfere with the lines you want to measure. Good luck.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
}
} ==============================Original Headers==============================
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} spectroscopy fixation
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
http://www.ansci.wisc.edu/microscopy.htm

==============================Original Headers==============================
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From: lukeclaire-at-yahoo.com
Date: Wed, 10 Aug 2005 10:25:08 -0500
Subject: [Microscopy] TEM of agar encapsulated sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Has anyone used a low melting point (LMP) agarose to
encapsulate cells for TEM? If yes, which resin is
best suited for LMP agarose encapsulted cells? We
have in the lab Durcupan ACM resin, EMbed-812, LRW
resin kits.

The samples were encapsulated after buffer wash, after
osmium fixation.

Thank you,

Claire

__________________________________________________
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==============================Original Headers==============================
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From: David.Patton-at-uwe.ac.uk
Date: Wed, 10 Aug 2005 10:45:23 -0500
Subject: [Microscopy] TEM of agar encapsulated sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We use Agarose type IX (Sigma). It works well with Spurrs and Araldite
(from TAAB in the UK). This summer I have had a problem infiltrating
cells in agarose with LR White (polymerising at 55 degrees C) despite
success in two previous years. This is probably a problem with me
rather than the products.

Dave

-----Original Message-----
X-from: lukeclaire-at-yahoo.com [mailto:lukeclaire-at-yahoo.com]
Sent: 10 August 2005 16:26
To: David Patton

Dear Listers,

Has anyone used a low melting point (LMP) agarose to
encapsulate cells for TEM? If yes, which resin is
best suited for LMP agarose encapsulted cells? We
have in the lab Durcupan ACM resin, EMbed-812, LRW
resin kits.

The samples were encapsulated after buffer wash, after
osmium fixation.

Thank you,

Claire

__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

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18, 30 -- From David.Patton-at-uwe.ac.uk Wed Aug 10 10:45:23 2005
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From: Elliott-at-arizona.edu
Date: Wed, 10 Aug 2005 10:53:34 -0500
Subject: [Microscopy] Re: TEM of agar encapsulated sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have used LMP many times for TEM. I process it just like I would a
block of tissue and go into EMbed-812 or Spurs.
Good luck
David


On Aug 10, 2005, at 8:29 AM, lukeclaire-at-yahoo.com wrote:

}
}
}
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}
} Dear Listers,
}
} Has anyone used a low melting point (LMP) agarose to
} encapsulate cells for TEM? If yes, which resin is
} best suited for LMP agarose encapsulted cells? We
} have in the lab Durcupan ACM resin, EMbed-812, LRW
} resin kits.
}
} The samples were encapsulated after buffer wash, after
} osmium fixation.
}
} Thank you,
}
} Claire
}
} __________________________________________________
} Do You Yahoo!?
} Tired of spam? Yahoo! Mail has the best spam protection around
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} ==============================Original
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} 6, 18 -- Date: Wed, 10 Aug 2005 08:25:07 -0700 (PDT)
} 6, 18 -- From: claire haueter {lukeclaire-at-yahoo.com}
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From: AOCHALSK-at-science.uottawa.ca
Date: Wed, 10 Aug 2005 11:14:17 -0500
Subject: [Microscopy] LM zinc-osmium staining of trout chloride cells:woes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

We have a group of labs that are
attempting to standardize an Osmium-
ZnI2 staining procedure for detection of
chloride cells in trout gills.

The protocol seems foolproof: immerse
freshly dissected gill pieces in a freshly-
made solution of 1 part 2% OsO4 to 4
parts 3% ZnI2 for 24 at 4ÚC, dehydrate,
clear in Histochoice, paraffin-embed,
section, de- paraffinize and mount in
Cytoseal60-xyl.
There seems to be no consistency in
results. In fact, more often or not we see
no staining of the chloride cells, though
the gill filaments themselves display the
rich tan colour of well-osmicated tissue.

Does anyone have experience with this
technique? What can cause it to fail? Is
age/source of reagents an important
factor? Are there any tricks to making
the procedure reliable?


==============================Original Headers==============================
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5, 21 -- Subject: LM zinc-osmium staining of trout chloride cells:woes
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From: tivol-at-caltech.edu
Date: Wed, 10 Aug 2005 12:35:18 -0500
Subject: [Microscopy] Re: TEM - liposome NTA bridge prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Aug 10, 2005, at 6:30 AM, lfox1-at-lumc.edu wrote:

} Can anyone advise this new user to our lab? His prep/questions are
} below. I think that he needs negative staining. How best to prep this
} sample?/fixation/washing out the sucrose?? and what type of filmed
} grids are being used these days??Formvar/Pioloform??other??
}
} -----------------------
}
} Basically what I have is a 100nm virus particle bound to a 100nm
} liposome molecule by a Nickel NTA "bridge". In short, I would like a
} picture of this complex. So, If I had this complex (usually in
} ~10-20%sucrose solution), what would I do from there as far a
} preparation?
}
Dear Linda,
If the specimen would not be perturbed by removal of the sucrose,
either of the techniques of negative staining or cryoEM would be
improved, and for negative staining with UAc, the buffer should not
contain phosphate. It would be best if the specimen could be in a
dilute buffer, either tris or a Good buffer would be suitable. I would
use carbon-formvar coated grids for negative staining and either lacy
carbon or, better, Quantifoils for cryo imaging. Depending on the
resolution desired, the tolerance of the specimen for low pH, the
equipment available, etc., the easiest procedure (and the one that I
would start with) is negative staining with UAc, which is at pH = ~3,
or phosphotungstate or phosphomolybdate, which are at pH = ~7. After
getting the specimen into the appropriate buffer, put 5 ul of the
appropriately diluted material onto a glow-discharged carbon-formvar
grid. Add 5 ul of a 2% solution of the staining compound, and let
stand for 1 min. Blot most of the liquid off with a bit of #1 filter
paper applied to the edge of the grid, add 10 ul of a 1% solution of
stain, let stand for 1 min, then thoroughly blot off the liquid. After
seeing whether the dilution of the specimen gives a good amount of
material on the grid--enough to see several particles in each (film or
CCD) frame--adjust the concentration if necessary. If cryoEM will be
useful, prepare the specimen at a concentration of about twice that
used for negative staining, and plunge-freeze it.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: baskin-at-bio.umass.edu
Date: Wed, 10 Aug 2005 13:14:29 -0500
Subject: [Microscopy] RE: TEM of agar encapsulated sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Claire,
Note that low melting point agarose (and probably all types)
is nicely stained by fast green. We would make a few percent solution
in 100% ethanol and add a drop to our agarose blobs. Otherwise, they
can vanish in the solutions and lead to some frustrations. I suppose
fast green would also dissolve in acetone but I don't know that for a
fact. However, now we trap small samples between formvar films on
wire loops and like that much better than agarose (except on the days
when the formvar won't cast, 8-().

HTH,
Tobias
}
}
} We use Agarose type IX (Sigma). It works well with Spurrs and Araldite
} (from TAAB in the UK). This summer I have had a problem infiltrating
} cells in agarose with LR White (polymerising at 55 degrees C) despite
} success in two previous years. This is probably a problem with me
} rather than the products.
}
} Dave
}
} -----Original Message-----
} X-from: lukeclaire-at-yahoo.com [mailto:lukeclaire-at-yahoo.com]
} Sent: 10 August 2005 16:26
} To: David Patton
} Subject: [Microscopy] TEM of agar encapsulated sample
}
}
} ----------------
} ----
}
} Dear Listers,
}
} Has anyone used a low melting point (LMP) agarose to
} encapsulate cells for TEM? If yes, which resin is
} best suited for LMP agarose encapsulted cells? We
} have in the lab Durcupan ACM resin, EMbed-812, LRW
} resin kits.
}
} The samples were encapsulated after buffer wash, after
} osmium fixation.
}
} Thank you,
}
} Claire
}
} __________________________________________________
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} 18, 30 -- From David.Patton-at-uwe.ac.uk Wed Aug 10 10:45:23 2005
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--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
4, 16 -- From baskin-at-bio.umass.edu Wed Aug 10 13:14:28 2005
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From: tiekotte-at-up.edu
Date: Wed, 10 Aug 2005 13:49:08 -0500
Subject: [Microscopy] viaWWW: Kodak Dektol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is not entirely true. Many years ago, I saw an article suggesting the
use of Dektol for developing 4489 and Electron Image Plates (glass). The
article demonstrated a more linear curve for exposure using 80-100kV.

I used Dektol for years after reading this article with great results. I
used this formula with SO-163: 5500 ML water + 400 ML Full strength Dektol.
I would basically make up the regular Dektol solution of photographic paper
development and from this solution would pour off 400 MLs. Consistent
results for printing with No. 3 grade paper were common.

You may have to experiment with scope intensity to exposure. I was able to
set-up my scope exposure was 2 seconds with a development of the regular 4
minutes exposure with nitrogen burst for 2 seconds every 10 seconds.

As for grain....what grain? Is a 3 x 4 foot enlargement ok for grain?

Ken
_______________________________________
Kenneth L. Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N Willamette Blvd.
Portland, OR 97203 USA

Tel.: 503.943.8861
Email: tiekotte-at-up.edu


On 8/10/05 6:30 AM, "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} That's an easy one.....no! Dektol is for developing photo paper, not
} film. Take it from someone who did this by mistake once and got
} negatives with grain the size of gravel and films so thick you could use
} them to view eclipses. It just might be possible with enough testing
} and fooling to get negatives you could actually see through, but the
} quality would be pretty awful anyway.
}
} If you don't make prints anymore, donate your Dektol to your local
} photography classes.
}
} Good luck,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
} -----Original Message-----
} X-from: Stacey.Andringa-at-uc.edu [mailto:Stacey.Andringa-at-uc.edu]
} Sent: Tuesday, August 09, 2005 10:40 PM
} To: Tindall, Randy D.
} Subject: [Microscopy] viaWWW: Kodak Dektol
}
}
}
}
} ------------------------------------------------------------------------
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} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (Stacey.Andringa-at-uc.edu) from
} http://www.microscopy.com/MLFormMail.html on Tuesday, August 9, 2005 at
} 12:35:33
} ------------------------------------------------------------------------
} ---
}
} Email: Stacey.Andringa-at-uc.edu
} Name: Stacey Andringa
}
} Organization: University of Cincinnati
}
} Title-Subject: [Filtered] MListserver:
}
} Question: We have bags of Kodak Dektol. Does anyone know if I can use
} this to develop Kodak Em film 4489? At what dilution, for how long?
} Thanks for any help.
}
} Stacey Andringa
}
} ------------------------------------------------------------------------
} ---
}
} ==============================Original
} Headers==============================
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} 19, 24 -- From TindallR-at-missouri.edu Wed Aug 10 08:30:28 2005
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} 19, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
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10, 19 -- From tiekotte-at-up.edu Wed Aug 10 13:49:08 2005
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From: tiekotte-at-up.edu
Date: Wed, 10 Aug 2005 14:31:04 -0500
Subject: [Microscopy] viaWWW: Kodak Dektol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Wow....I stand corrected. In 40 years of darkroom work, this is the
first I have heard of using Dektol successfully for film development,
except for a few avante-garde photographers using it for special effects
(i.e., grain). My life has been more sheltered than I thought, I guess.

I take it you have never used it with 4489? Just curious.

Randy



-----Original Message-----
X-from: Ken Tiekotter [mailto:tiekotte-at-up.edu]
Sent: Wednesday, August 10, 2005 1:50 PM
To: microscopy-at-microscopy.com; Stacey Andringa
Cc: Tindall, Randy D.

Randy,
I did use it with 4489, but liked the linearity of SO-163 compared with
Kodak plate film and so switched to SO-163. I no longer use film or the
darkroom as I have gone 100% digital.

Ken


On 8/10/05 11:58 AM, "Tindall, Randy D." {TindallR-at-missouri.edu} wrote:

} Wow....I stand corrected. In 40 years of darkroom work, this is the
} first I have heard of using Dektol successfully for film development,
} except for a few avante-garde photographers using it for special effects
} (i.e., grain). My life has been more sheltered than I thought, I guess.
}
} I take it you have never used it with 4489? Just curious.
}
} Randy
}
}
}
} -----Original Message-----
} From: Ken Tiekotter [mailto:tiekotte-at-up.edu]
} Sent: Wednesday, August 10, 2005 1:50 PM
} To: microscopy-at-microscopy.com; Stacey Andringa
} Cc: Tindall, Randy D.
} Subject: Re: [Microscopy] RE: viaWWW: Kodak Dektol
}
} This is not entirely true. Many years ago, I saw an article suggesting
} the use of Dektol for developing 4489 and Electron Image Plates (glass).
} The article demonstrated a more linear curve for exposure using
} 80-100kV.
}
} I used Dektol for years after reading this article with great results.
} I used this formula with SO-163: 5500 ML water + 400 ML Full strength
} Dektol.
} I would basically make up the regular Dektol solution of photographic
} paper development and from this solution would pour off 400 MLs.
} Consistent results for printing with No. 3 grade paper were common.
}
} You may have to experiment with scope intensity to exposure. I was able
} to set-up my scope exposure was 2 seconds with a development of the
} regular 4 minutes exposure with nitrogen burst for 2 seconds every 10
} seconds.
}
} As for grain....what grain? Is a 3 x 4 foot enlargement ok for grain?
}
} Ken
} _______________________________________
} Kenneth L. Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N Willamette Blvd.
} Portland, OR 97203 USA
}
} Tel.: 503.943.8861
} Email: tiekotte-at-up.edu
}
}
} On 8/10/05 6:30 AM, "TindallR-at-missouri.edu" {TindallR-at-missouri.edu}
} wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------
} } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
}
} } of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------
} } ------
} }
} } That's an easy one.....no! Dektol is for developing photo paper, not
}
} } film. Take it from someone who did this by mistake once and got
} } negatives with grain the size of gravel and films so thick you could
} } use them to view eclipses. It just might be possible with enough
} } testing and fooling to get negatives you could actually see through,
} } but the quality would be pretty awful anyway.
} }
} } If you don't make prints anymore, donate your Dektol to your local
} } photography classes.
} }
} } Good luck,
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility---We Do Small Well!
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-2227
} } Email: tindallr-at-missouri.edu
} } Web: http://www.emc.missouri.edu
} }
} }
} }
} }
} }
} } -----Original Message-----
} } X-from: Stacey.Andringa-at-uc.edu [mailto:Stacey.Andringa-at-uc.edu]
} } Sent: Tuesday, August 09, 2005 10:40 PM
} } To: Tindall, Randy D.
} } Subject: [Microscopy] viaWWW: Kodak Dektol
} }
} }
} }
} }
} } ----------------------------------------------------------------------
} } --
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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} } ----------------------------------------------------------------------
} } --
} } ----
} }
} } Below is the result of your feedback form (NJZFM-ultra-55). It was
} } submitted by (Stacey.Andringa-at-uc.edu) from
} } http://www.microscopy.com/MLFormMail.html on Tuesday, August 9, 2005
} } at
} } 12:35:33
} } ----------------------------------------------------------------------
} } --
} } ---
} }
} } Email: Stacey.Andringa-at-uc.edu
} } Name: Stacey Andringa
} }
} } Organization: University of Cincinnati
} }
} } Title-Subject: [Filtered] MListserver:
} }
} } Question: We have bags of Kodak Dektol. Does anyone know if I can use
} } this to develop Kodak Em film 4489? At what dilution, for how long?
} } Thanks for any help.
} }
} } Stacey Andringa
} }
} } ----------------------------------------------------------------------
} } --
} } ---
} }
} } ==============================Original
} } Headers==============================
} } 7, 12 -- From zaluzec-at-microscopy.com Tue Aug 9 22:37:06 2005 7, 12 --
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} } way of MicroscopyListserver) 7, 12 -- Subject: viaWWW: Kodak Dektol 7,
}
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} } 19, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 19, 24 --
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==============================Original Headers==============================
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6, 18 -- Date: Wed, 10 Aug 2005 12:31:29 -0700
6, 18 -- Subject: Re: [Microscopy] RE: viaWWW: Kodak Dektol
6, 18 -- From: Ken Tiekotter {tiekotte-at-up.edu}
6, 18 -- To: "Tindall, Randy D." {TindallR-at-missouri.edu}
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From: stefan.diller-at-t-online.de
Date: Wed, 10 Aug 2005 20:00:19 -0500
Subject: [Microscopy] viaWWW: soap structures in lubricants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (stefan.diller-at-t-online.de) from http://www.microscopy.com/MLFormMail.html on Wednesday, August 10, 2005 at 10:38:26
---------------------------------------------------------------------------

Email: stefan.diller-at-t-online.de
Name: Stefan Diller

Title-Subject: [Filtered] Soap structure

Question: Hello,
is anybody out there willing to share the secrets how to image soap structures in lubricants?
Or how to get the oil out of the soap structure for doing SEM work?
Is there any publication available in this field of work?
Please feel free to contact me offline.

Best regards,
Stefan Diller


----------------------------------------------------------------------------
-----------------------------------------
Stefan Diller - Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49 - 931 - 7848700 Phone
++49 - 931 - 7848701 Fax
++49 - 175 - 717 70 51 Cell-Phone
----------------------------------------------------------------------------

---------------------------------------------------------------------------

==============================Original Headers==============================
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==============================End of - Headers==============================




From: smalinskas-at-yahoo.com
Date: Thu, 11 Aug 2005 07:44:57 -0500
Subject: [Microscopy] Re: viaWWW: soap structures in lubricants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stefan,

One possible way is by using the Quantomix Wet-SEM
technology.

http://www.quantomix.com/

I haven't used their system, but it may be one
possible way to image lubricant soap structures.

Stu Smalinskas, P.E.
Metallurgist
SKF North American Technical Center
Plymouth, Michigan
(734) 414-6862

--- stefan diller wrote:

}
} Email: stefan.diller-at-t-online.de
} Name: Stefan Diller
}
} Title-Subject: [Filtered] Soap structure
}
} Question: Hello,
} is anybody out there willing to share the secrets
} how to image soap structures in lubricants?
} Or how to get the oil out of the soap structure for
} doing SEM work?
} Is there any publication available in this field of
} work?
} Please feel free to contact me offline.
}
} Best regards,
} Stefan Diller
}
}
} -----------------------------------------
} Stefan Diller - Photography
} Arndtstrasse 22
} D - 97072 Wuerzburg Germany
} ++49 - 931 - 7848700 Phone
} ++49 - 931 - 7848701 Fax
} ++49 - 175 - 717 70 51 Cell-Phone


__________________________________________________
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==============================Original Headers==============================
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9, 19 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
9, 19 -- Subject: Re: [Microscopy] viaWWW: soap structures in lubricants
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From: estyer-at-uga.edu
Date: Thu, 11 Aug 2005 09:00:05 -0500
Subject: [Microscopy] viaWWW: drying ethanol or acetone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (estyer-at-uga.edu) from http://www.microscopy.com/MLFormMail.html on Thursday, August 11, 2005 at 08:59:18
---------------------------------------------------------------------------

Email: estyer-at-uga.edu
Name: Eloise L. Styer

Organization: University of Georgia

Title-Subject: [Filtered] drying ethanol or acetone

Question: I would like to change our TEM tissue embeddment protocol to eliminate propylene oxide. Toward that end, what are some effective (and quick and easy...) methods to remove water from "100%" ethanol or acetone? We have an extremely humid building environment and I am sure there are many better techniques than my ancient method of adding the appropriate molecular sieve, swirling and letting stand until the fine particulates settle...

Thanks in advance.

Eloise---

Dr. Eloise L. Styer
Veterinary Diagnostic Lab
University of Georgia
43 Brighton Road
POB 1389
Tifton, GA 31793

Phone 229-386-3340
Fax 229-386-7128
e-mail estyer-at-uga.edu

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: lukeclaire-at-yahoo.com
Date: Thu, 11 Aug 2005 11:14:49 -0500
Subject: [Microscopy] TEM of agar encapsulated samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Many thanks to all of you who provided input on TEM
processing for agar encapsulated samples.

Also, the article by Paul Webster in Microscopy Today
shows insightful techniques to process small pellets,
samples. Thank you for pointing me to that article.

Have a nice day,

Claire



__________________________________
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==============================Original Headers==============================
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From: bozzola-at-siu.edu
Date: Thu, 11 Aug 2005 13:06:44 -0500
Subject: [Microscopy] Re: viaWWW: drying ethanol or acetone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For many years now we have been purchasing sealed, pint containers of
100% ethanol. We use freshly opened containers as the final ethanol.
After several openings, this is then considered as 95% ethanol and
used to produced the more dilute ethanol series. We have never had a
failed embedding due to water in the ethanol using this procedure.
And, yes, we are also in a humid environment.




} Email: estyer-at-uga.edu
} Name: Eloise L. Styer
}
} Organization: University of Georgia
}
} Title-Subject: [Filtered] drying ethanol or acetone
}
} Question: I would like to change our TEM tissue embeddment protocol
} to eliminate propylene oxide. Toward that end, what are some
} effective (and quick and easy...) methods to remove water from
} "100%" ethanol or acetone? We have an extremely humid building
} environment and I am sure there are many better techniques than my
} ancient method of adding the appropriate molecular sieve, swirling
} and letting stand until the fine particulates settle...
}
} Thanks in advance.
}
} Eloise---
}
} Dr. Eloise L. Styer
} Veterinary Diagnostic Lab
} University of Georgia
} 43 Brighton Road
} POB 1389
} Tifton, GA 31793
}
} Phone 229-386-3340
} Fax 229-386-7128
} e-mail estyer-at-uga.edu
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 10, 12 -- From zaluzec-at-microscopy.com Thu Aug 11 09:00:04 2005
} 10, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
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From: hoffpajo-at-yahoo.com
Date: Thu, 11 Aug 2005 13:12:05 -0500
Subject: [Microscopy] drying ethanol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

how does one make 100% etoh dryer? isn't it already
dry? or did i miss something in my organic chemistry?

__________________________________________________
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==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Thu, 11 Aug 2005 13:45:19 -0500
Subject: [Microscopy] Re: drying ethanol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i know this, the original question was how to dry out
100% etoh and ethanol. yes of course 100% etoh will
absorb moisture from the air and how much will depend
on the humidity and lenght of time the bottle is open.
i would think in modern air conditioned buildings
would have a fairly low humidity.
john

--- Jan Factor {jfactor-at-ns.purchase.edu} wrote:

} 100% EtOH immediately begins to remove water from
} the air (in an attempt
} to dry out the atmosphere, which is exactly what it
} does when it dries
} tissues), so as soon as you open it, it is no longer
} 100%. Drying agents
} (such as copper sulfate) are usually added to sop up
} the water as it
} enters the solution.
} --Jan Factor
}
} ---------------------------------------
} Jan Robert Factor, Ph.D.
} Professor of Biology
} ---------------------------------------
} Natural Sciences
} Purchase College
} State University of New York
} 735 Anderson Hill Rd.
} Purchase, NY 10577
} USA
} ---------------------------------------
} Office Tel: 914-251-6659
} Office Fax: 914-251-6635
} E-mail: jfactor-at-ns.purchase.edu
} or- jan.factor-at-purchase.edu
} ---------------------------------------
}
}
} hoffpajo-at-yahoo.com wrote:
} }
}
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} }
} } how does one make 100% etoh dryer? isn't it
} already
} } dry? or did i miss something in my organic
} chemistry?
} }
} } __________________________________________________
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} } Tired of spam? Yahoo! Mail has the best spam
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==============================Original Headers==============================
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From: ech-at-interchange.ubc.ca
Date: Thu, 11 Aug 2005 18:43:32 -0500
Subject: [Microscopy] viaWWW: microwave protocols for electron and light microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ech-at-interchange.ubc.ca) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, August 11, 2005 at 11:29:45
---------------------------------------------------------------------------

Email: ech-at-interchange.ubc.ca
Name: Elaine Humphrey

Organization: UBC

Title-Subject: [Filtered] MListserver: microwave protocols for electron and light microscopy

Question: Hello Everyone
The microwave protocols website has moved from http://www.microwaveprep.org to
http://www.microwaveprotocols.ubc.ca

Debby Sherman did an awesome job organizing the original website but didn't have the time to maintain it and has passed it on to me since I have someone who maintains my website. (Note: my webmaster wanted to use CSS. If you have a Mac there are still some problems using Internet Explorer but it works just fine in Safari. If anyone has any experience with CSS bugs for Mac IE, please get in touch with me.)

We started to use a microwave four years ago. It became so popular we now have two.

For confocal and fluorescent microscopy where a protocol normally takes three hours (an hour in the primary antibody and an hour in the secondary), it typically takes a total of half an hour in the microwave. So researchers come in at 9 am and are on the confocal at 9.30 am. We usually find less background staining and the cells are fresher - especially when the primary staining is usually overnight.

For em processing that normally takes about three days, it is 2-3 hours in the microwave.

If it works conventionally, it will probably work in the microwave. However, the settings tend to be different for different specimens. What works for one doesn't necessarily work for another. Hence the Microwave Protocols website as a depository for different protocols and tips. For instance, a PhD student here was using the microwave for fluorescent staining of brain slices and found that staining 30 micron sections in eppendorf tubes stained better than 10-15 micron sections in a 24 well plate.

If you have a protocol that works for a particular specimen, please submit it to anyone of the reviewers. We have a review panel to check the protocols before they go up.

Elaine Humphrey, Director of the BioImaging Facility,
The University of British Columbia, Vancouver, CA
ech-at-interchange.ubc.ca

Kent McDonald, Director of the Electron Microscopy Lab,
University of California, Berkeley
klm-at-uclink4.berkeley.ed

Rick Webb, Centre for Microscopy and Microanalysis,
University of Queensland, Brisbane, AU
r.webb-at-uq.edu.au

Ronald Austin, Health Sciences Center,
Louisiana State University, Shreveport, LA
RAusti-at-lsuhsc.edu

Richard Giberson, Biomedical R&D,
Ted Pella, Inc., Redding, CA
rick_giberson-at-tedpella.com

Paul Webster, Ahmanson Advanced EM & Imaging Center,
House Ear Institute, Los Angeles, CA
pwebster-at-hei.org

Jonathan Day, Department of Biology,
California State University, Chico, Chico, CA
jday-at-csuchico.edu

If anyone wants to try using the microwave, there is going to be a demo/workshop in the next Scanning meeting to be held in Washington DC next April.

Elaine

--
Dr. Elaine Humphrey
Director, BioImaging Facility
President, Microscopy Society of Canada (2003-2005)
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-interchange.ubc.ca
website: www.emlab.ubc.ca


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23, 12 -- Subject: viaWWW: microwave protocols for electron and light microscopy
23, 12 -- Content-Type: text/plain; charset="us-ascii"
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From: s2kdude-at-pacbell.net
Date: Thu, 11 Aug 2005 18:44:18 -0500
Subject: [Microscopy] viaWWW: T-12 communication

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (s2kdude-at-pacbell.net) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, August 11, 2005 at 13:10:47
---------------------------------------------------------------------------

Email: s2kdude-at-pacbell.net
Name: willy

Title-Subject: [Filtered] MListserver: T-12 communication

Question: I need to know if anyone out there has had success in connecting an external PC that runs a ccd camera to a T-12 TEM. Is it possible to get communication such as mag and kV this way?

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: Bplowman-at-pacific.edu
Date: Thu, 11 Aug 2005 18:44:55 -0500
Subject: [Microscopy] viaWWW: Sputtering from Gold Coin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Bplowman-at-pacific.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, August 11, 2005 at 13:23:32
---------------------------------------------------------------------------

Email: Bplowman-at-pacific.edu
Name: Barbara Plowman

Organization: Univ. of the Pacific/Arthur A. Dugoni School of Dentistry

Title-Subject: [Filtered] Sputtering from Gold Coin

Question: Dear Listserver,
Awhile back there was a string about gold sputtering using a gold coin. This was considerably cheaper than buying a new target. I don't know how to find these in the archives. Do any of you know how this was done or if you know where this information is located in the archives of the Listserver? I have a Hummer V. Thanks. Barbara Plowman P.S. I hope this isn't a repeat post!


Barbara Plowman
Univ. of the Pacific
Arthur A. Dugoni School of Dentistry
2155 Webster Rm 642
San Francisco, CA
Bplowman-at-pacific.edu
415-929-6692

---------------------------------------------------------------------------

==============================Original Headers==============================
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8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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8, 12 -- Content-Type: text/plain; charset="us-ascii"
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From: Rosemary.White-at-csiro.au
Date: Thu, 11 Aug 2005 22:31:30 -0500
Subject: [Microscopy] Re: viaWWW: Sputtering from Gold Coin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Barbara,
Although probably more expensive than using a gold coin, you pay not much
more than the current price for gold if you get your local jeweller to make
up a target. We get ours made somewhat thicker than specified so they last
longer. The donation of a sputter-coated insect or other item is usually
much appreciated too!
cheers,
rosemary

Dr. Rosemary White rosemary.white-at-csiro.au
Microscopy Centre ph. 61-2-6246 5475
CSIRO Plant Industry mob. 61-0402 835 973
GPO Box 1600 fax. 61-2-6246 5334
Canberra, ACT 2601
Australia

} From: Bplowman-at-pacific.edu
} Reply-To: Bplowman-at-pacific.edu
} Date: Thu, 11 Aug 2005 18:47:47 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] viaWWW: Sputtering from Gold Coin
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted
} by (Bplowman-at-pacific.edu) from
} http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday,
} August 11, 2005 at 13:23:32
} ---------------------------------------------------------------------------
}
} Email: Bplowman-at-pacific.edu
} Name: Barbara Plowman
}
} Organization: Univ. of the Pacific/Arthur A. Dugoni School of Dentistry
}
} Title-Subject: [Filtered] Sputtering from Gold Coin
}
} Question: Dear Listserver,
} Awhile back there was a string about gold sputtering using a gold coin. This
} was considerably cheaper than buying a new target. I don't know how to find
} these in the archives. Do any of you know how this was done or if you know
} where this information is located in the archives of the Listserver? I have a
} Hummer V. Thanks. Barbara Plowman P.S. I hope this isn't a repeat post!
}
}
} Barbara Plowman
} Univ. of the Pacific
} Arthur A. Dugoni School of Dentistry
} 2155 Webster Rm 642
} San Francisco, CA
} Bplowman-at-pacific.edu
} 415-929-6692
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 11 18:44:54 2005
} 8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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} 8, 12 -- To: microscopy-at-microscopy.com
} 8, 12 -- From: Bplowman-at-pacific.edu (by way of MicroscopyListserver)
} 8, 12 -- Subject: viaWWW: Sputtering from Gold Coin
} 8, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
3, 23 -- From Rosemary.White-at-csiro.au Thu Aug 11 22:31:30 2005
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3, 23 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0
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3, 23 -- Subject: Re: [Microscopy] viaWWW: Sputtering from Gold Coin
3, 23 -- From: Rosemary White {Rosemary.White-at-csiro.au}
3, 23 -- To: {Bplowman-at-pacific.edu} , {Microscopy-at-microscopy.com}
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From: hoffpajo-at-yahoo.com
Date: Fri, 12 Aug 2005 11:23:37 -0500
Subject: [Microscopy] Re: drying ethanol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

yes i know, i know. i was responding to a poorly
written question that stated they wanted to dry out
100% etoh.


--- MICHAEL J DELANNOY {mdelann1-at-jhem.jhmi.edu} wrote:

}
} According to the Warner-Graham Co. (my supplier)
} the 100% ethanol is already molecular sieved so
} should
} be free of water when you open it. Of course
} humidity
} in the lab would contaminate opened bottles. If you
} want
} to molecular sieve this stock, about 1 inch of
} molecular
} sieve 3 A 4-8 mesh beads, let it settle (few days)
} until clear.
} My supplier did mention this might form ethylene
} dichloride,
} not sure how
}
} M Delannoy
} ----- Original Message -----
} From: hoffpajo-at-yahoo.com
} Date: Thursday, August 11, 2005 2:15 pm
} Subject: [Microscopy] drying ethanol
}
} }
} }
} }
} }
}
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} } AmericaTo Subscribe/Unsubscribe --
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} }
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} }
} } how does one make 100% etoh dryer? isn't it
} already
} } dry? or did i miss something in my organic
} chemistry?
} }
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} (PDT)
} } 2, 18 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
} } 2, 18 -- Subject: drying ethanol
} } 2, 18 -- To: microscopy-at-microscopy.com
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__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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6, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 19 -- Subject: Re: [Microscopy] drying ethanol
6, 19 -- To: MICHAEL J DELANNOY {mdelann1-at-jhem.jhmi.edu} , microscopy-at-microscopy.com
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From: walck-at-southbaytech.com
Date: Fri, 12 Aug 2005 12:40:31 -0500
Subject: [Microscopy] Disc grinder mounting stub dimensions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Could someone please tell me the height of the Gatan 3/8" diameter
mounting stubs for their Disc Grinder? I am making an adapter for our
Dimpler(R) for a customer who uses that hand grinder and the dimensions
are critical. Obviously in my new position, I don't have one on hand
(or in my hand). If you could also verify the diameters for both the
stainless steel and Pyrex(R) mounts it would be greatly appreciated.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


==============================Original Headers==============================
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4, 25 -- To: {Microscopy-at-microscopy.com}
4, 25 -- Subject: Disc grinder mounting stub dimensions
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From: wood-at-pw.usda.gov
Date: Fri, 12 Aug 2005 14:26:13 -0500
Subject: [Microscopy] large format negative scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Scott,
My Gatan Disc Grinder 623 has two mounting stubs and one is about 3/8" long
and the other a little (~1/16") longer. They were probably both longer when
it was new, but of course they get ground down a little as you use the tool.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
X-from: {walck-at-southbaytech.com}
To: {mager-at-interchange.ubc.ca}
Sent: Friday, August 12, 2005 10:44 AM

I'm looking for a multi format negative scanner (like my now broken
Polaroid Sprintscan 45). Any recommendations? I'd like to be able to scan
35mm, 4"x5" negs and 99 mm x 81 mm (EM negs) and microscope slides.

Thanks,

De Wood
USDA ARS WRRC
800 Buchanan St.
Albany, CA 94710
(510) 559-5653


==============================Original Headers==============================
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From: cornheadorama-at-hotmail.com
Date: Sat, 13 Aug 2005 08:29:24 -0500
Subject: [Microscopy] viaWWW: Working ISI Mini-SEM Console/ TV Scan May Be Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MLFormMail.html on Saturday, August 13, 2005 at 04:06:04
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: Techutopia

Title-Subject: [Filtered] MListserver: Working ISI Mini-SEM Console/ TV Scan May Be Available.

Question: Hi,

As some of you may have noticed on Usenet, I've been developing a digital/pc replacement for the "bulky" console of the diminutive ISI Mini-SEM. I hope to be able to bring the 'scope around to primary and secondary schools in hopes of inspiring kids into pursuing science.

The project is almost finished and it's time to decide what to do with the console. It's temtpting to canabalize it for the ~$150 in AMP connectors I'll need to finish my project, but before I tore into it I wanted to see if there was any interest from someone with a viable 'scope and problematic console. Only the console, TV scan controller, and little B/W monitor is included: I need the external power supply.

I still have some testing and analysis to do, probably be about a month before it's ready to ship.

Contact me if you're interested,

-Jeff


---------------------------------------------------------------------------

==============================Original Headers==============================
12, 12 -- From zaluzec-at-microscopy.com Sat Aug 13 08:29:24 2005
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12, 12 -- To: microscopy-at-microscopy.com
12, 12 -- From: cornheadorama-at-hotmail.com (by way of MicroscopyListserver)
12, 12 -- Subject: viaWWW: Working ISI Mini-SEM Console/ TV Scan May Be Available
12, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: cornheadorama-at-hotmail.com
Date: Sat, 13 Aug 2005 08:30:07 -0500
Subject: [Microscopy] viaWWW: ISI Mini-SEM Supplies/Upgrades/Accessories Wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://microscopy.com/MLFormMail.html on Saturday, August 13, 2005 at 04:25:30
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Organization: Techutopia

Title-Subject: [Filtered] MListserver: ISI Mini-SEM Supplies/Upgrades/Accessories Wanted

Question: Hi,


I'm interested in purchasing accessories, upgrades, crossgrades, and parts for ISI MINI-SEM units. In particular I'm looking for alternate chambers and stage
arrangements. I have the "straight-on" chamber based on right angles: curious about the chamber with the 45 degree cut for the controls.

Filaments, apertures, liners, any other running gear always appreciated.

The 'scope will probably spend about %50 of it's on-hours in primary and secondary schools for the next few years, at least.

Anyone with extra stuff, or who might have a lead on a repository, please e-mail.

-Jeff


-Jeff


---------------------------------------------------------------------------

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From: hbarwood-at-troy.edu
Date: Sat, 13 Aug 2005 10:00:08 -0500
Subject: [Microscopy] Mechanical repair help?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

My experience with a wide range of sputter coaters would suggest that
provided the gold coin was in good electrical contact with the power supply
(perhaps glued to an old target with a conducting media) you should find
everything works well.

Good Luck

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
X-from: {Bplowman-at-pacific.edu}
To: {protrain-at-emcourses.com}
Sent: Friday, August 12, 2005 12:45 AM

I tried having this part repaired, but got scammed. Fortunately, I have the
part back, if not my money. If anyone has a source of ball bearings, I will
try and fix it myself. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu

-----Original Message-----
X-from: hbarwood-at-troy.edu [mailto:hbarwood-at-troy.edu]
Sent: Thursday, July 21, 2005 2:04 PM
To: hbarwood-at-troy.edu

I have an old Simplex microscope (actually Leitz,) with one of the massive
stands used for this type of measuring microscope. The micrometer height
adjustment went out on me years ago and I've just been using the threaded
height adjustor on the column to focus the scope. Now, I'm at a point where
I really need the fine adjustment. Is there anyone out there who can
disassemble the focusing rack that holds the scope and replace the small
ball bearings so it will function again? If anyone can help, please let me
know. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu


==============================Original Headers==============================
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==============================Original Headers==============================
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From: becks-at-sunynassau.edu
Date: Sat, 13 Aug 2005 12:26:38 -0500
Subject: [Microscopy] EM 300 Main Relay

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The main relay (RE-1) on my Philips EM 300 has quit on me and I'm
looking for anyone who might have spare parts for the EM 300 (for
sale or donation). I have images of the relay that I can send offline
if you need help in identifying the part.

Thanks in advance for any assistance!

Steve
--
Stephen J. Beck
Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}

==============================Original Headers==============================
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4, 18 -- Date: Sat, 13 Aug 2005 13:26:36 -0400
4, 18 -- From: Steve Beck {becks-at-sunynassau.edu}
4, 18 -- Subject: EM 300 Main Relay
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From: hoffpajo-at-yahoo.com
Date: Sat, 13 Aug 2005 20:36:33 -0500
Subject: [Microscopy] Mechanical repair help?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

a very quick even yahoo search for ball bearing
yielded a at least a few companyies, try this one for
ball bearings: www.bocabearings.com
and i have no vested interest in this company

--- hbarwood-at-troy.edu wrote:

}
}
}
}
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}
} I tried having this part repaired, but got scammed.
} Fortunately, I have the
} part back, if not my money. If anyone has a source
} of ball bearings, I will
} try and fix it myself. Thanks.
}
} Henry Barwood
} Associate Professor of Science, Earth Science
} Department of Math and Physics
} MSCX 312G
} Troy University
} Troy, Alabama 36082
} hbarwood-at-troy.edu
}
} -----Original Message-----
} X-from: hbarwood-at-troy.edu [mailto:hbarwood-at-troy.edu]
} Sent: Thursday, July 21, 2005 2:04 PM
} To: hbarwood-at-troy.edu
} Subject: [Microscopy] Mechanical repair help?
}
}
}
}
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}
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}
} I have an old Simplex microscope (actually Leitz,)
} with one of the massive
} stands used for this type of measuring microscope.
} The micrometer height
} adjustment went out on me years ago and I've just
} been using the threaded
} height adjustor on the column to focus the scope.
} Now, I'm at a point where
} I really need the fine adjustment. Is there anyone
} out there who can
} disassemble the focusing rack that holds the scope
} and replace the small
} ball bearings so it will function again? If anyone
} can help, please let me
} know. Thanks.
}
} Henry Barwood
} Associate Professor of Science, Earth Science
} Department of Math and Physics
} MSCX 312G
} Troy University
} Troy, Alabama 36082
} hbarwood-at-troy.edu
}
}
} ==============================Original
} Headers==============================
} 3, 25 -- From hbarwood-at-troy.edu Thu Jul 21 14:02:29
} 2005
} 3, 25 -- Received: from webshield01.troy.edu
} (scan.troy.edu
} [198.179.130.124])
} 3, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with



From: cgarber-at-2spi.com
Date: Sun, 14 Aug 2005 13:23:08 -0500
Subject: [Microscopy] Gold coins and sputter coater cathodes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Steve Chapman wrote:
===============================================================
My experience with a wide range of sputter coaters would suggest that
provided the gold coin was in good electrical contact with the power supply
(perhaps glued to an old target with a conducting media) you should find
everything works well.
===============================================================
Steve is correct if you were using a coin made of pure gold, probably better
than 0.999 purity.

But it has to have that high of a purity to work. Why? Because with gold
being so soft, most (monetary) coins (in fact all that I have ever heard of)
contain alloying elements that don't sputter so well (or at all). Hence,
once a little gold is sputtered, the surface becomes rich in these (left
over) alloying elements and sputtering stops. One does not need very much
of the typical alloying elements before such difficulties present
themselves. Therefore, in the general case, "gold" coins really don't work
very well. We have also seen some evidence of particulate contamination of
the samples from small metal inclusions that rain down on the sample once
freed from the gold matrix. The exception is the Canadian Gold Maple Leaf
which is advertised as being 0.9999 pure and that, should work quite nicely
provided it is not too thick (2.8 mm) to fit into your cathode holder.
However most coaters don't like cathodes that thick, and they are designed
to take cathode diameters larger than the 30 mm Maple Leaf.

If the reason why one is motivated to try using a coin is to save money, a
better approach might be to visit a jewelry workshop and you should be able
to get a gold foil of the needed purity. But then again it tends to not be
in the most desirable thickness and it also would have to be cut into the
needed disc diameter with some amount of waste foil (and some of the
"savings"). You would also lose the opportunity to return the left over
cathode for recycling (for a recycling discount) as is possible with some
of the microscopy supply vendors who routinely supply replacement gold
cathodes for coaters. It is the mechanical processing and "cookie cutting"
of the gold cathodes, and polishing, that results in replacement cathodes
from traditional sources to appear "higher" in price relative to the jewelry
store option.

Disclaimer: SPI Supplies offers replacement gold and other targets for
sputter coaters and we would have a vested interest in having prospective
customers purchase their replacement cathodes from firms like ours.

Chuck

===========================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




==============================Original Headers==============================
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11, 25 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com}
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From: peterd-at-pmail.ntu.edu.sg
Date: Mon, 15 Aug 2005 08:14:30 -0500
Subject: [Microscopy] viaWWW: Steps to XPS data Interpretation

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (peterd-at-pmail.ntu.edu.sg) from http://www.microscopy.com/MLFormMail.html on Monday, August 15, 2005 at 00:13:04
---------------------------------------------------------------------------

Email: peterd-at-pmail.ntu.edu.sg
Name: Peter Darmawan

Organization: Nanyang Technological University

Title-Subject: [Filtered] MListserver: Steps to XPS data Interpretation

Question: Dear All,

I am a new graduate student, switching from Mechanical Engineering to Materials engineering.

I need some help in interpreting XPS data which I got. So far I could not find any help on the internet with regard to the data interpretation and books on XPS is limited at my school library. Any help would be greatly appreciated.

Thank you,

Peter

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: as-at-astonmet.com
Date: Mon, 15 Aug 2005 08:14:54 -0500
Subject: [Microscopy] Re: Gold coins and sputter coater cathodes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We used a Canadian Maple Leaf several years and have had great success. We
pounded it flat and used silver paste for the adhesive.

Alan Stone
ASTON


At 01:27 PM 8/14/2005, you wrote:



} ----------------------------------------------------------------------------
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Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com


==============================Original Headers==============================
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From: estyer-at-uga.edu
Date: Mon, 15 Aug 2005 19:03:59 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Thanks drying ethanol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think you sent your e'mail to the wrong person. I don't think I know
you.

kathleen

-----Original Message-----
X-from: c.jeffree-at-ed.ac.uk [mailto:c.jeffree-at-ed.ac.uk]
Sent: Tuesday, August 09, 2005 2:27 AM
To: Greer, Kathleen P

I don't care
Chris

----- Original Message -----
X-from: {luc-at-anaspec.co.za}
To: {cjeffree-at-staffmail.ed.ac.uk}
Sent: Tuesday, August 09, 2005 8:22 AM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (estyer-at-uga.edu) from http://www.microscopy.com/MLFormMail.html on Monday, August 15, 2005 at 08:36:14
---------------------------------------------------------------------------

Email: estyer-at-uga.edu
Name: Eloise L. Styer

Organization: University of Georgia

Title-Subject: [Filtered] drying ethanol

Question: Dear All,

Thanks for your suggestions. They were all appreciated. Well, all that did not simply find fault with my reference to "100%" alcohol. And here I thought that putting the 100% in quotations would make it clear that I was not referring to absolutely 100% alcohol...

Eloise---


Dr. Eloise L. Styer
Veterinary Diagnostic Lab.
University of Georgia
43 Brighton Road
POB 1389
Tifton, GA 31793

Phone 229-386-3340
Fax 229-386-7128
Email estyer-at-uga.edu

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From: mkomboli-at-uno.edu
Date: Mon, 15 Aug 2005 19:04:23 -0500
Subject: [Microscopy] viaWWW: LR gold and benzoyl peroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mkomboli-at-uno.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, August 15, 2005 at 10:36:18
---------------------------------------------------------------------------

Email: mkomboli-at-uno.edu
Name: Mary Kombolias

Organization: University of New Orleans

Title-Subject: [Filtered] LR gold and benzoyl peroxide

Question: Hi! I was wondering how I should catalyze LR Gold with benzoyl peroxide. The LR Gold resin that I purchased did not come with any instructions on how to add the catalyst. The blurb in the Ted Pella catalogue only mentioned "benzoyl peroxide, 1%w/v." My question is, do I add the benzoyl peroxide to the entire bottle of LR Gold the same way the catalyst is added to the entire bottle of LR White resin or do I add the benzoyl peroxide to whatever aliquot of LR Gold resin I am using for each project?

Thanks!

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Mon, 15 Aug 2005 19:43:32 -0500
Subject: [Microscopy] Re: viaWWW: LR gold and benzoyl peroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

try this for instructions
http://www.polysciences.com/shop/assets/datasheets/641.pdf

--- mkomboli-at-uno.edu wrote:

}
}
}
}
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} Below is the result of your feedback form
} (NJZFM-ultra-55). It was submitted by
} (mkomboli-at-uno.edu) from
}
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} on Monday, August 15, 2005 at 10:36:18
}
---------------------------------------------------------------------------
}
} Email: mkomboli-at-uno.edu
} Name: Mary Kombolias
}
} Organization: University of New Orleans
}
} Title-Subject: [Filtered] LR gold and benzoyl
} peroxide
}
} Question: Hi! I was wondering how I should catalyze
} LR Gold with benzoyl peroxide. The LR Gold resin
} that I purchased did not come with any instructions
} on how to add the catalyst. The blurb in the Ted
} Pella catalogue only mentioned "benzoyl peroxide,
} 1%w/v." My question is, do I add the benzoyl
} peroxide to the entire bottle of LR Gold the same
} way the catalyst is added to the entire bottle of LR
} White resin or do I add the benzoyl peroxide to
} whatever aliquot of LR Gold resin I am using for
} each project?
}
} Thanks!
}
}
---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
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} 19:04:22 2005
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} MicroscopyListserver)
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} peroxide
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}


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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==============================Original Headers==============================
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From: guy-at-emu.usyd.edu.au
Date: Mon, 15 Aug 2005 22:47:32 -0500
Subject: [Microscopy] viaWWW: Sydney/Seefeld cryo workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Subject is: Sydney/Seefeld cryo workshop

The third of the popular Seefeld in Sydney Workshops
on Cryotechniques in Electron Microscopy will be
held at the Electron Microscope Unit, University of
Sydney on 24th - 28th October 2005.

That means that there is just over one month to
go until priority registration closes on September
23rd. After that date a place cannot be guaranteed.

The course includes standard cryo-fixation techniques,
cryo-ultramicrotomy and immunogold labelling, and will
introduce the participants to nano-cryo techniques including
automated vitrification, advanced cryo-imaging, cryo-EM
tomography,single particle analysis, and electron
crystallography.

It assumes prior knowledge of basic specimen preparation
for electron microscopy, general ultramicrotomy and basic
antibody staining.

Course instructors include:
Dr Jan Leunissen, Managing Director Aurion and
Director R&D EM Unit, Otago University, NZ
Ross Boadle, Senior Scientist, E. M. Laboratory,
Westmead Millenium Inst. and ICPMR Westmead
Dr Ben Hankamer, Inst. for Molecular Bioscience,
University of Queensland
Assoc. Prof.Guy Cox, E.M.Unit, University of Sydney
Assoc. Prof.Filip Braet, Deputy Director, E.M. Unit,
University of Sydney
Anne Simpson, Specimen Preparation Manager,E.M. Unit,
University of Sydney
Graham Tranter, Emgrid Australia
Dr Teresa Dibbayawan, Leica Microsystems
Jocelyn Carpenter, Nanotechnology Systems

Equipment available will include Leica High Pressure Freezer,
Automatic Freeze Substitution and Cryo-ultramicrotomes and
FEI Vitrobot system.

Registration is $A1,000 (commercial) and $A850 (education),
which includes lunches and morning and afternoon tea

This course is run by NANO (Nanostructural Analysis Network
Organisation) and NANO subscribers receive a discounted rate.

We gratefully acknowlege the sponsorship of Leica, FEI and
Nanotechnology Systems.

For more details email
seefeld.cryo-at-emu.usyd.edu.au
or go to:
_______________________________________________________

http://www.nano.org.au http://www.emu.usyd.edu.au
_______________________________________________________

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From: shashis_99-at-yahoo.com
Date: Mon, 15 Aug 2005 23:08:30 -0500
Subject: [Microscopy] Re: viaWWW: LR gold and benzoyl peroxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr. Komboli,
Just add the required amount of Benzoyl peroxide in
an aliquot of LR gold (the amount you require) and
stir it. It would dissolve then you can embed your
samples and polymerise. I use about 50 mg for 5ml of
LR gold.
shashi singh
CCMB Hyderabad
INDIA

--- mkomboli-at-uno.edu wrote:

}
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}
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}
} Email: mkomboli-at-uno.edu
} Name: Mary Kombolias
}
} Organization: University of New Orleans
}
} Title-Subject: [Filtered] LR gold and benzoyl
} peroxide
}
} Question: Hi! I was wondering how I should catalyze
} LR Gold with benzoyl peroxide. The LR Gold resin
} that I purchased did not come with any instructions
} on how to add the catalyst. The blurb in the Ted
} Pella catalogue only mentioned "benzoyl peroxide,
} 1%w/v." My question is, do I add the benzoyl
} peroxide to the entire bottle of LR Gold the same
} way the catalyst is added to the entire bottle of LR
} White resin or do I add the benzoyl peroxide to
} whatever aliquot of LR Gold resin I am using for
} each project?
}
} Thanks!
}
}
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}
} ==============================Original
} Headers==============================
} 7, 12 -- From zaluzec-at-microscopy.com Mon Aug 15
} 19:04:22 2005
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} peroxide
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==============================Original Headers==============================
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5, 20 -- From: shashi singh {shashis_99-at-yahoo.com}
5, 20 -- Subject: Re: [Microscopy] viaWWW: LR gold and benzoyl peroxide
5, 20 -- To: mkomboli-at-uno.edu
5, 20 -- Cc: microscopy-at-msa.microscopy.com
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From: hinmeigeng-at-hotmail.com
Date: Tue, 16 Aug 2005 13:33:58 -0500
Subject: [Microscopy] Optical Microscopes: Leica v Olympus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello again listers,

A former colleague, now in another continent, has sent me the following
query. Could any of you please share your experiences on ANY of the aspects
here: if you feel there's the likelihood of a commercial flame war
developing, please send the reply to me only, but any thoughts of general
interest please reply to the Listserver.

Thanks for your attention.

* * * * * * * * * * * * * * *

I have some microscopy questions again, wondering if you can help? I am
going to buy an optical microscope for our institute, of course, my students
will use it more than others. I expect to use it in transmission Polarized
mode (for liquid crystal, polymer crystallization etc) and reflective
Nomarski interference contrast mode (for polymer surface and other surface
morphology) (still under consideration are the fluorescent mode combined
with our existing monochromator and CCD spectrometer)

I have contacted two companies, Leica and Olympus. However, as the price for
Leica is higher than Olympus, it is very hard for me to make a decision for
which company to go. I don't know if I can have some advice from you? I am
not familiar with Olympus and not sure the quality of it.

Leica has put on market many new models. The one I bought in the UK was a
Research type model. Leica here has an upgraded research model, but many new
low cost analytical models, cheaper on the body. At the moment, their
research model is still very expensive. I may have difficulty to afford it.
However, I am not sure of the quality of these analytical models. Leica
low cost polarized model has its analyser resolved only at 2-3 degree, but
in their research model the analyser can resolve to 0.1 degree. The
analytical model can also be fitted with reflective Interference Contrast
(via Smith reflector and DIC prisms)

I was told Olympus developed from biology microscopy and now is capable to
fit Nomarski Optics (they call it differential interference contrast DIC). I
have seen a research type Olympus microscope today. It seems quite good to
me. As I do not have sample to try, I checked only quality of the cross
polarizer. It seems the polarizer quality is good.

What Olympus argue that they can provide better quality of long working
distance lens than Leica to work with LINKAM hotstage. Leica provide me only
N PLAN lens for both polarized and interference contrast. Olympus suggest
these lenses are not good enough for interference contrast.

The choice of Lens seems very important. However, Leica Agents have not
advised me on that so far. I don't have must experience on choosing lens. Do
you also have any suggestions on that? I am looking forward to hearing from
you.

* * * * * * EOF * * * * * *

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



==============================Original Headers==============================
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From: amr2w-at-virginia.edu
Date: Tue, 16 Aug 2005 14:39:15 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It was over 5 years ago that I shopped for a Pol Scope. But I think our
strategy will still work.

1. We collected a set of slides representing the type of things we would or
might want to see through the scope. In our case it included hairs, molds,
insects, crystals, etc.

2. We prepared an evaluation sheet with each feature we want to look at or
check, each specimen to examine, also the manufacturer, model demonstrated,
costs, etc.

3. We gave each available vendor a separate day to demo their scope using
our set of slides & any they supplied.

4. We had 2-3 evaluators who filled out an evaluation sheet for each scope
we looked at.

5. Later we compared the evaluations & discussed it as a group. We took
into account price, quality of scope, features, how well it worked with our
slide set, responsiveness & attitude of salespeople to our requests &
questions, availability & cost of service, warranty length, etc before
making our recommendations to our supervisor & lab director.

Hope that helps.

David A. Foran, chemist
Food and Drug Administration
Kansas City Elemental Analysis Lab
DFORAN-at-ORA.FDA.GOV
913-752-2170
913-752-2151 (fax)

-----Original Message-----
X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com]
Sent: Tuesday, August 16, 2005 1:36 PM
To: Foran, David A

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amr2w-at-virginia.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 16, 2005 at 13:47:01
---------------------------------------------------------------------------

Email: amr2w-at-virginia.edu
Name: Andrew Roelant

Organization: University of Virginia

Title-Subject: [Filtered] LM Digital Camera Recommendations?

Question: Hey All,
I've decided to update the digital camera on our Olympus PME 3 invertedlight microscope. The digital camera that is currently attached is a Kodak DMC I (digital microscope camera). Any suggestions what to get that won't break the bank? I'm going for the best resolution for the buck. I've heard of people buying regular digital cameras and kits to attach to microscopes rather than the specialized microscope cameras; I don't know which gives better pictures/scheaper. I just need better resolution than what we currently have. Being able to control the camera from the computer and import directly into photoshop is nice also. Thanks in advance for the advice!
-Andrew Roelant
Graduate Research Assistant
Department of Materials Science and Engineering
University of Virginia

---------------------------------------------------------------------------

==============================Original Headers==============================
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6, 12 -- From: amr2w-at-virginia.edu (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: LM Digital Camera Recommendations?
6, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: hoffpajo-at-yahoo.com
Date: Tue, 16 Aug 2005 14:44:05 -0500
Subject: [Microscopy] Re: viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


i like the nikon D-70 digital camera. it can easily
fitted to most microscopes or as for my use a
telescope with a t mount adaptor.
john
--- amr2w-at-virginia.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
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} Below is the result of your feedback form
} (NJZFM-ultra-55). It was submitted by
} (amr2w-at-virginia.edu) from
} http://www.microscopy.com/MLFormMail.html on
} Tuesday, August 16, 2005 at 13:47:01
}
---------------------------------------------------------------------------
}
} Email: amr2w-at-virginia.edu
} Name: Andrew Roelant
}
} Organization: University of Virginia
}
} Title-Subject: [Filtered] LM Digital Camera
} Recommendations?
}
} Question: Hey All,
} I've decided to update the digital camera on our
} Olympus PME 3 invertedlight microscope. The digital
} camera that is currently attached is a Kodak DMC I
} (digital microscope camera). Any suggestions what to
} get that won't break the bank? I'm going for the
} best resolution for the buck. I've heard of people
} buying regular digital cameras and kits to attach to
} microscopes rather than the specialized microscope
} cameras; I don't know which gives better
} pictures/scheaper. I just need better resolution
} than what we currently have. Being able to control
} the camera from the computer and import directly
} into photoshop is nice also. Thanks in advance for
} the advice!
} -Andrew Roelant
} Graduate Research Assistant
} Department of Materials Science and Engineering
} University of Virginia
}
}
---------------------------------------------------------------------------
}
} ==============================Original
} Headers==============================
} 6, 12 -- From zaluzec-at-microscopy.com Tue Aug 16
} 14:39:15 2005
} 6, 12 -- Received: from [206.69.208.22]
} (mac22.zaluzec.com [206.69.208.22])
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} ESMTP id j7GJdEnj010005
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} 6, 12 -- To: microscopy-at-microscopy.com
} 6, 12 -- From: amr2w-at-virginia.edu (by way of
} MicroscopyListserver)
} 6, 12 -- Subject: viaWWW: LM Digital Camera
} Recommendations?
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____________________________________________________
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==============================Original Headers==============================
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6, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 19 -- Subject: Re: [Microscopy] viaWWW: LM Digital Camera Recommendations?
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From: rcsaic-at-sbcglobal.net
Date: Wed, 17 Aug 2005 09:38:37 -0500
Subject: [Microscopy] TEM Sample Prep: Plasma Cleaning Ultramicrotomy Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are interested in feedback from any group in the community who has
experience with plasma cleaning TEM samples prepared by ultramicrotomy. Our
specific samples are inorganic (mineral plus some carbonaceous material)
grains in epoxy ultramicrotomy sections suppoted on continuous carbon film
TEM grids. We would like to know whether contamination can be successfully
prevented, or removed, from these samples by plasma cleaning (while in the
TEM holder) without also causing destruction, alteration or volatilization
of the epoxy section and/or carbon support film.



Thanks,



Roy Christoffersen

SAIC

NASA Johnson Space Center EM Facilities


==============================Original Headers==============================
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From: edelmare-at-muohio.edu
Date: Wed, 17 Aug 2005 10:14:25 -0500
Subject: [Microscopy] Re: viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For years we have been using a Kodak DCS760 (actually a
Nikon F5 SLR body with Kodak CDD and electronics). The SLR's
are very nice because (1) they mount on the photo ports correctly
(i.e. F-mount, and otehrs), (2) they use the microscopes optics
alone, (3) and the SLR's have the better end (consumer/prosumer)
CCD's and electronics. Negative side of things is that unlike true
scieitific cameras the exposure systems are not setup to handle
Light Microsopy type imaging very well and so a little fiddling is
required.

We just got a new Nikon D-50 (very similar to the D-70, CCD
noise seems a little better, and $100 lower cost). With the Nikon
Capture software it can be used in "tethered" mode and driven
completely by the computer. Now, when I say we just got, I really
mean that, the box arrived this morning and we haven't installed it
yet. Give me a couple of days and I will give a report back.

As for breaking the bank: Nikon D-50 body $720, Capture
software (needed for tethered operation) $100, and right angle
viewfinder $185.

Right angle view finder: Very VERY helpful for upright
microscopes, but may not be needed / useful if you can use the
front camera port on an inverted scope.

More info on the Nikon & other digital camers take a look at:

http://www.dpreview.com/reviews/specs/Nikon/


(BTW: I do not sell cameras or anything else nor have any financial
interests in digital cameras or Nikon)


On 16 Aug 2005, at 14:40, amr2w-at-virginia.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (amr2w-at-virginia.edu) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 16, 2005 at 13:47:01
} ---------------------------------------------------------------------------
}
} Email: amr2w-at-virginia.edu
} Name: Andrew Roelant
}
} Organization: University of Virginia
}
} Title-Subject: [Filtered] LM Digital Camera Recommendations?
}
} Question: Hey All,
} I've decided to update the digital camera on our Olympus PME 3 invertedlight microscope. The digital camera that is currently attached is a Kodak DMC I (digital microscope camera). Any suggestions what to get that won't break the bank? I'm going for the best resolution for the buck. I've
heard of people buying regular digital cameras and kits to attach to microscopes rather than the specialized microscope cameras; I don't know which gives better pictures/scheaper. I just need better resolution than what we currently have. Being able to control the camera from the computer and
import directly into photoshop is nice also. Thanks in advance for the advice!
} -Andrew Roelant
} Graduate Research Assistant
} Department of Materials Science and Engineering
} University of Virginia
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 6, 12 -- From zaluzec-at-microscopy.com Tue Aug 16 14:39:15 2005
} 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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} 6, 12 -- From: amr2w-at-virginia.edu (by way of MicroscopyListserver)
} 6, 12 -- Subject: viaWWW: LM Digital Camera Recommendations?
} 6, 12 -- Content-Type: text/plain; charset="us-ascii"
} ==============================End of - Headers==============================



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Wed, 17 Aug 2005 10:25:32 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


for high quality images everything depends on the
pixels. the more pixels the better the image.
as for exposeures, everyone needs to remember all
cameras ""see"" things as an 18% grey card. so all
images need to be adjusted.
and yes to all those of you about to jump on me i know
this is simplistic.
--- edelmare-at-muohio.edu wrote:

}
}
}
}
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}
} For years we have been using a Kodak DCS760
} (actually a
} Nikon F5 SLR body with Kodak CDD and electronics).
} The SLR's
} are very nice because (1) they mount on the photo
} ports correctly
} (i.e. F-mount, and otehrs), (2) they use the
} microscopes optics
} alone, (3) and the SLR's have the better end
} (consumer/prosumer)
} CCD's and electronics. Negative side of things is
} that unlike true
} scieitific cameras the exposure systems are not
} setup to handle
} Light Microsopy type imaging very well and so a
} little fiddling is
} required.
}
} We just got a new Nikon D-50 (very similar to the
} D-70, CCD
} noise seems a little better, and $100 lower cost).
} With the Nikon
} Capture software it can be used in "tethered" mode
} and driven
} completely by the computer. Now, when I say we just
} got, I really
} mean that, the box arrived this morning and we
} haven't installed it
} yet. Give me a couple of days and I will give a
} report back.
}
} As for breaking the bank: Nikon D-50 body $720,
} Capture
} software (needed for tethered operation) $100, and
} right angle
} viewfinder $185.
}
} Right angle view finder: Very VERY helpful for
} upright
} microscopes, but may not be needed / useful if you
} can use the
} front camera port on an inverted scope.
}
} More info on the Nikon & other digital camers take a
} look at:
}
} http://www.dpreview.com/reviews/specs/Nikon/
}
}
} (BTW: I do not sell cameras or anything else nor
} have any financial
} interests in digital cameras or Nikon)
}
}
} On 16 Aug 2005, at 14:40, amr2w-at-virginia.edu wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
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} Microscopy Society of America
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} }
}
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} }
} } Below is the result of your feedback form
} (NJZFM-ultra-55). It was submitted by
} (amr2w-at-virginia.edu) from
} http://www.microscopy.com/MLFormMail.html on
} Tuesday, August 16, 2005 at 13:47:01
} }
}
---------------------------------------------------------------------------
} }
} } Email: amr2w-at-virginia.edu
} } Name: Andrew Roelant
} }
} } Organization: University of Virginia
} }
} } Title-Subject: [Filtered] LM Digital Camera
} Recommendations?
} }
} } Question: Hey All,
} } I've decided to update the digital camera on
} our Olympus PME 3 invertedlight microscope. The
} digital camera that is currently attached is a Kodak
} DMC I (digital microscope camera). Any suggestions
} what to get that won't break the bank? I'm going for
} the best resolution for the buck. I've
} heard of people buying regular digital cameras and
} kits to attach to microscopes rather than the
} specialized microscope cameras; I don't know which
} gives better pictures/scheaper. I just need better
} resolution than what we currently have. Being able
} to control the camera from the computer and
} import directly into photoshop is nice also. Thanks
} in advance for the advice!
} } -Andrew Roelant
} } Graduate Research Assistant
} } Department of Materials Science and Engineering
} } University of Virginia
} }
} }
}
---------------------------------------------------------------------------
} }
} } ==============================Original
} Headers==============================
} } 6, 12 -- From zaluzec-at-microscopy.com Tue Aug 16
} 14:39:15 2005
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} (mac22.zaluzec.com [206.69.208.22])
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} } 6, 12 -- From: amr2w-at-virginia.edu (by way of
} MicroscopyListserver)
} } 6, 12 -- Subject: viaWWW: LM Digital Camera
} Recommendations?
} } 6, 12 -- Content-Type: text/plain;
} charset="us-ascii"
} } ==============================End of -
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}
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 350 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
} ==============================Original
} Headers==============================
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} 10:14:14 2005
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} 15, 23 -- From: "Richard E. Edelmann"
} {edelmare-at-muohio.edu}
} 15, 23 -- To: amr2w-at-virginia.edu,
} microscopy-at-Microscopy.com
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} Digital Camera Recommendations?
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From: sergei2-at-ornl.gov
Date: Wed, 17 Aug 2005 10:42:03 -0500
Subject: [Microscopy] SPM Postdoctoral Position - Center for Nanophase Materials Science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Center for Nanophase Materials Science (CNMS) and the Condensed Matter Sciences Division of Oak Ridge National Laboratory invite applications for an experimental postdoctoral position research in development and application of advanced scanning probe microscopies (SPM) to complex materials and to support the user-initiated nanoscience research program of the CNMS. The successful applicant should have demonstrated experience in the application, development, and interpretation of modern SPM techniques, such as acoustic, electromechanical, electrochemical, transport imaging or imaging in a liquid environment.  The applicant should have a Ph.D. in materials science, physics, or related field, and be capable of interacting with a wide range of users probing the nanoscale properties of a diverse range of materials including polymers and other soft materials, semiconductors, and biological systems. This position provides an opportunity to take advantage of new state-of-the-art facilities at the Center for Nanophase Materials Science, including a NanoFabrication Center and advanced scanning based probes and to interact with ongoing programs in the Low Dimensional Materials by Design and first-principles theory groups at ORNL. The complete information on this position is available on the CNMS web-site: http://cnms.ornl.gov/postdoc_research/CNMS_PS.shtm
 
Sergei
--
Sergei V. Kalinin,
Oak Ridge National Laboratory,
1 Bethel Valley Rd,
Bldg. 3025, MS6030,
Oak Ridge, TN 37831
Phone: (865) 241-0236
FAX: (865) 574-4143
URL: sergei2.kalininweb.com
New: http://nanotransport.ornl.gov
 


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From: Mike.Bode-at-soft-imaging.net
Date: Wed, 17 Aug 2005 10:49:15 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think this post is too simplistic. Don't play the Pixel game when you buy a scientific camera. For example: If you buy an 8 Mpixel camera to work at high magnification on your microscope, you are wasting money and storage. The resolution will be limited by the microscope, and not by the number of pixels of the camera. Likewise, if you are doing Fluorescence, a camera with fewer pixels can be more sensitive and thus be superior to a camera with gobs of pixels.

Also, please explain your comment that "all cameras ""see"" things as an 18% grey card".

mike

Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: hoffpajo-at-yahoo.com [mailto:hoffpajo-at-yahoo.com]
Sent: Wednesday, August 17, 2005 9:28 AM
To: Mike Bode


for high quality images everything depends on the pixels. the more pixels the better the image.
as for exposeures, everyone needs to remember all cameras ""see"" things as an 18% grey card. so all images need to be adjusted.
and yes to all those of you about to jump on me i know this is simplistic.
--- edelmare-at-muohio.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
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}
} For years we have been using a Kodak DCS760 (actually a Nikon F5 SLR
} body with Kodak CDD and electronics).
} The SLR's
} are very nice because (1) they mount on the photo ports correctly
} (i.e. F-mount, and otehrs), (2) they use the microscopes optics alone,
} (3) and the SLR's have the better end
} (consumer/prosumer)
} CCD's and electronics. Negative side of things is that unlike true
} scieitific cameras the exposure systems are not setup to handle Light
} Microsopy type imaging very well and so a little fiddling is required.
}
} We just got a new Nikon D-50 (very similar to the D-70, CCD noise
} seems a little better, and $100 lower cost).
} With the Nikon
} Capture software it can be used in "tethered" mode and driven
} completely by the computer. Now, when I say we just got, I really
} mean that, the box arrived this morning and we haven't installed it
} yet. Give me a couple of days and I will give a report back.
}
} As for breaking the bank: Nikon D-50 body $720, Capture software
} (needed for tethered operation) $100, and right angle viewfinder
} $185.
}
} Right angle view finder: Very VERY helpful for upright microscopes,
} but may not be needed / useful if you can use the front camera port on
} an inverted scope.
}
} More info on the Nikon & other digital camers take a look at:
}
} http://www.dpreview.com/reviews/specs/Nikon/
}
}
} (BTW: I do not sell cameras or anything else nor have any financial
} interests in digital cameras or Nikon)
}
}
} On 16 Aug 2005, at 14:40, amr2w-at-virginia.edu wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
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} }
}
----------------------------------------------------------------------------
} }
} } Below is the result of your feedback form
} (NJZFM-ultra-55). It was submitted by
} (amr2w-at-virginia.edu) from
} http://www.microscopy.com/MLFormMail.html on Tuesday, August 16, 2005
} at 13:47:01
} }
}
---------------------------------------------------------------------------
} }
} } Email: amr2w-at-virginia.edu
} } Name: Andrew Roelant
} }
} } Organization: University of Virginia
} }
} } Title-Subject: [Filtered] LM Digital Camera
} Recommendations?
} }
} } Question: Hey All,
} } I've decided to update the digital camera on
} our Olympus PME 3 invertedlight microscope. The digital camera that is
} currently attached is a Kodak DMC I (digital microscope camera). Any
} suggestions what to get that won't break the bank? I'm going for the
} best resolution for the buck. I've heard of people buying regular
} digital cameras and kits to attach to microscopes rather than the
} specialized microscope cameras; I don't know which gives better
} pictures/scheaper. I just need better resolution than what we
} currently have. Being able to control the camera from the computer and
} import directly into photoshop is nice also. Thanks in advance for the
} advice!
} } -Andrew Roelant
} } Graduate Research Assistant
} } Department of Materials Science and Engineering University of
} } Virginia
} }
} }
}
---------------------------------------------------------------------------
} }
} } ==============================Original
} Headers==============================
} } 6, 12 -- From zaluzec-at-microscopy.com Tue Aug 16
} 14:39:15 2005
} } 6, 12 -- Received: from [206.69.208.22]
} (mac22.zaluzec.com [206.69.208.22])
} } 6, 12 -- by ns.microscopy.com (8.12.11/8.12.8)
} with ESMTP id j7GJdEnj010005
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} } microscopy-at-microscopy.com 6, 12 -- From: amr2w-at-virginia.edu (by way
} } of
} MicroscopyListserver)
} } 6, 12 -- Subject: viaWWW: LM Digital Camera
} Recommendations?
} } 6, 12 -- Content-Type: text/plain;
} charset="us-ascii"
} } ==============================End of -
} Headers==============================
}
}
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Director
} 350 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
} http://www.emf.muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
} ==============================Original
} Headers==============================
} 15, 23 -- From edelmare-at-muohio.edu Wed Aug 17
} 10:14:14 2005
} 15, 23 -- Received: from mulnx12.mcs.muohio.edu
} (mulnx12.mcs.muohio.edu [134.53.6.67])
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} with ESMTP id j7HFEEWY015687
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} 15, 23 -- Wed, 17 Aug 2005 11:14:09 -0400
} 15, 23 -- From: "Richard E. Edelmann"
} {edelmare-at-muohio.edu}
} 15, 23 -- To: amr2w-at-virginia.edu,
} microscopy-at-Microscopy.com
} 15, 23 -- Date: Wed, 17 Aug 2005 11:14:08 -0400 15, 23 --
} MIME-Version: 1.0 15, 23 -- Content-type: text/plain; charset=US-ASCII
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From: zaluzec-at-aaem.amc.anl.gov
Date: Wed, 17 Aug 2005 11:01:38 -0500
Subject: [Microscopy] Re: TEM Sample Prep: Plasma Cleaning Ultramicrotomy Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roy

We routinely plasma clean holey/continuous carbon films with
particles on them it works
fine here at ANL. I've only done a few (i.e. { 5) microtomed
samples, but have not had
any problems with them, but that is not a significant number of
samples to gauge the
effectiveness.

The key here is to recognize, as you have apparently already done,
that the reactive plasma obviously will attack the carbon in your
support as well as the source of hydrocarbon contamination. I have found
that the hydrocarbon, not surprizingly is more reactive than, for
example, the more
stable carbon support films. The key here is to tailor your gas composition
as well as to adjust the power levels of your plasma. At ANL, for
carbon support films, we
use Argon gas only and at a low power setting of ~ 5-10 W for ~ 10 minutes
in our SBT PC-2000 plasma cleaner. In my experience, for these type of samples,
you should keep oxygen to a minimum as this will rapidly attach all
carbon. Basically,
we have found that there will be enough residual oxygen released by
the Argon plasma
to attack the organic contamination, assuming the specimen is not
heavily coated.

Of course, not all "carbon support films", are created equal and
some have more
hydrocarbon than others, so you will need to test the receipe for your films
as well as for the model of plasma cleaner and its settings
as each manufactureres unit will vary in efficacy. It is best here to make
a few sacrifical films and tune your settings to minimze the reaction on your
support films.

Nestor
Your Friendly Neighborhood SysOp

Disclaimer: My employer Argonne National Lab holds the basic patent
on TEM/SEM plasma
cleaning technology and licenses this to manufacturers of commerical units.


} ----------------------------------------------------------------------------
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--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================

==============================Original Headers==============================
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12, 16 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
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From: hoffpajo-at-yahoo.com
Date: Wed, 17 Aug 2005 11:04:09 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unless i have forgetten basics of camera exposures,
which is possible i am getting old. the metter in the
camera takes all the light falling on it and
"averages" it. it assumes the image to be aboout 18%
grey card. if you don't have one i can send you one of
mine. i use them to handle high contrast subjects.
--- Mike.Bode-at-soft-imaging.net wrote:

}
}
}
}
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}
} I think this post is too simplistic. Don't play the
} Pixel game when you buy a scientific camera. For
} example: If you buy an 8 Mpixel camera to work at
} high magnification on your microscope, you are
} wasting money and storage. The resolution will be
} limited by the microscope, and not by the number of
} pixels of the camera. Likewise, if you are doing
} Fluorescence, a camera with fewer pixels can be more
} sensitive and thus be superior to a camera with gobs
} of pixels.
}
} Also, please explain your comment that "all cameras
} ""see"" things as an 18% grey card".
}
} mike
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:  (888) FIND SIS
}         (303) 234-9270
} fax:    (303) 234-9271
} email:  mailto:info-at-soft-imaging.com
} web:    http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: hoffpajo-at-yahoo.com
} [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 9:28 AM
} To: Mike Bode
} Subject: [Microscopy] viaWWW: LM Digital Camera
} Recommendations?
}
}
}
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] RE: viaWWW: LM Digital Camera Recommendations?
5, 19 -- To: Mike.Bode-at-soft-imaging.net, microscopy-at-microscopy.com
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From: hoffpajo-at-yahoo.com
Date: Wed, 17 Aug 2005 11:30:30 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

again unless i am out of it isn't the highest
resolution possible to be about 200nm in a light
microscope? and pixel resolution to be between 3 to
8um, i could be wrong. again very simplistic. so i
guess i should hang onto my D-70.
--- Mike.Bode-at-soft-imaging.net wrote:

}
}
}
}
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} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
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} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
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}
} I think this post is too simplistic. Don't play the
} Pixel game when you buy a scientific camera. For
} example: If you buy an 8 Mpixel camera to work at
} high magnification on your microscope, you are
} wasting money and storage. The resolution will be
} limited by the microscope, and not by the number of
} pixels of the camera. Likewise, if you are doing
} Fluorescence, a camera with fewer pixels can be more
} sensitive and thus be superior to a camera with gobs
} of pixels.
}
} Also, please explain your comment that "all cameras
} ""see"" things as an 18% grey card".
}
} mike
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:  (888) FIND SIS
}         (303) 234-9270
} fax:    (303) 234-9271
} email:  mailto:info-at-soft-imaging.com
} web:    http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: hoffpajo-at-yahoo.com
} [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 9:28 AM
} To: Mike Bode
} Subject: [Microscopy] viaWWW: LM Digital Camera
} Recommendations?
}
}
}
}
}
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4, 19 -- Subject: Re: [Microscopy] RE: viaWWW: LM Digital Camera Recommendations?
4, 19 -- To: Mike.Bode-at-soft-imaging.net, microscopy-at-microscopy.com
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From: Mike.Bode-at-soft-imaging.net
Date: Wed, 17 Aug 2005 11:42:35 -0500
Subject: Re: [Microscopy] RE: viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

200 nm is proabaly a bit optimistic, but let's go with that value. If you use a 100x lens, the 200 nm spot will be enlarged to 20 microns. If you use an 8 Mpixel camera with a pixel size of 2 microns or so, you are oversampling by a large factor.

Now, there are compelling reasons to buy an 8 Mpixel camera, but the number of pixels is not the only parameter you should use to decide on a camera.


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john hoffpauir [mailto:hoffpajo-at-yahoo.com]
Sent: Wednesday, August 17, 2005 10:30 AM
To: Mike Bode; microscopy-at-microscopy.com


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==============================Original Headers==============================
11, 24 -- From Mike.Bode-at-soft-imaging.net Wed Aug 17 11:42:35 2005
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From: Mike.Bode-at-soft-imaging.net
Date: Wed, 17 Aug 2005 11:47:05 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hmmm, you must be thinking on film cameras. In digital cameras, you don't have an extra sensor for exposure time setting. The imaging sensor itelf provides the information that you need. This allows a much better control of the exposure. For example you could take the information from the live image that is displayed on the camera or monitor, and find the maximum and minimum exposure pixels and then calculate a new exposure time to maximize signal, or minimize noise, etc. You can do that for the entire sensor, only use a certain spot, average over several spots...


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: hoffpajo-at-yahoo.com [mailto:hoffpajo-at-yahoo.com]
Sent: Wednesday, August 17, 2005 10:07 AM
To: Mike Bode


unless i have forgetten basics of camera exposures, which is possible i am getting old. the metter in the camera takes all the light falling on it and "averages" it. it assumes the image to be aboout 18% grey card. if you don't have one i can send you one of mine. i use them to handle high contrast subjects.
--- Mike.Bode-at-soft-imaging.net wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} I think this post is too simplistic. Don't play the Pixel game when
} you buy a scientific camera. For
} example: If you buy an 8 Mpixel camera to work at high magnification
} on your microscope, you are wasting money and storage. The resolution
} will be limited by the microscope, and not by the number of pixels of
} the camera. Likewise, if you are doing Fluorescence, a camera with
} fewer pixels can be more sensitive and thus be superior to a camera
} with gobs of pixels.
}
} Also, please explain your comment that "all cameras ""see"" things as
} an 18% grey card".
}
} mike
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:  (888) FIND SIS
}         (303) 234-9270
} fax:    (303) 234-9271
} email:  mailto:info-at-soft-imaging.com
} web:    http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: hoffpajo-at-yahoo.com
} [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 9:28 AM
} To: Mike Bode
} Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?
}
}
}
}
}
----------------------------------------------------------------------------
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5, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 19 -- Subject: Re: [Microscopy] RE: viaWWW: LM Digital Camera Recommendations?
5, 19 -- To: Mike.Bode-at-soft-imaging.net, microscopy-at-microscopy.com
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From: hoffpajo-at-yahoo.com
Date: Wed, 17 Aug 2005 11:57:07 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

that is the reason i like the d-70. it allows you to
sample the exposure over several spots. i also have
software purchased the adobe creative suite 2. it alos
for a great deal of control over the final image, such
as image bracking. i highly recomend it.

well i learned photography with a film camrea. i still
have several including a large format camera.
i have no vested interest in either nikon or adobe.
just like them both.

--- Mike.Bode-at-soft-imaging.net wrote:

}
}
}
}
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} Hmmm, you must be thinking on film cameras. In
} digital cameras, you don't have an extra sensor for
} exposure time setting. The imaging sensor itelf
} provides the information that you need. This allows
} a much better control of the exposure. For example
} you could take the information from the live image
} that is displayed on the camera or monitor, and find
} the maximum and minimum exposure pixels and then
} calculate a new exposure time to maximize signal, or
} minimize noise, etc. You can do that for the entire
} sensor, only use a certain spot, average over
} several spots...
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:  (888) FIND SIS
}         (303) 234-9270
} fax:    (303) 234-9271
} email:  mailto:info-at-soft-imaging.com
} web:    http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: hoffpajo-at-yahoo.com
} [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 10:07 AM
} To: Mike Bode
} Subject: [Microscopy] viaWWW: LM Digital Camera
} Recommendations?
}
}
}
}
}
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From: wesaia-at-iastate.edu
Date: Wed, 17 Aug 2005 12:05:26 -0500
Subject: [Microscopy] Re: viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Supposing the resolution is 200 nm, that is less than half a wavelength of
light. That might be attainable given a lens (and other optics) of
sufficiently high NA. Those are generally the higher power objectives. My
basic 40x lens has an NA of only 0.63, so I won't get such good resolution.
But suppose we could get 200 nm out of a 100x objective.

200 nm resolution means we would need pixels every 100 nm or so. I
generally figure prints of 120 mm or so wide. That means the width of my
image will be about 120 um at 1000x. That means I will need 1200 pixels
across my image for a 100-nm pixel spacing. That isn't very many pixels in
today's terms. My old 1.3 megapixel Pixera can handle that.

You might say that the extra pixels allow you to take lower magnification
images and still record up to the resolution limit. First, what is the
resolution for that set of lenses? The NA and resolution falls off with a
drop in magnification. There may not be detail to record with the extra
pixels.

Having said all that, 3 megapixel consumer cameras are practically throw
away items now. For that matter, so are 40 GB disk drives. It doesn't hurt
to add a few more pixels (and MB) to be safe. However, I think a lot of
people are quick to take high-pixel images for which they never will use
the information contained there. They just provide a demand for more and
bigger hard drives.

I am going to have to run a comparison here one of these days. I have a gut
feeling that lower pixel number cameras may be more sensitive in low-light
situations than their higher pixel cousins. (I think that is what Mike Bode
suggested.) I want to do a side-by-side comparison to show myself and
others. I will grant that higher pixel cameras can record more detail in
their regular photographic mode under sufficient light. I just don't know
that they have the same edge in the lab.

Warren Straszheim
(adding my name here so you don't have to check out the address line above
or below)

At 11:31 AM 08/17/05, John H. wrote:

} again unless i am out of it isn't the highest
} resolution possible to be about 200nm in a light
} microscope? and pixel resolution to be between 3 to
} 8um, i could be wrong. again very simplistic. so i
} guess i should hang onto my D-70.
} --- Mike.Bode-at-soft-imaging.net wrote:
}
} }
} }
} }
} }
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } To Subscribe/Unsubscribe --
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} } On-Line Help
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} } I think this post is too simplistic. Don't play the
} } Pixel game when you buy a scientific camera. For
} } example: If you buy an 8 Mpixel camera to work at
} } high magnification on your microscope, you are
} } wasting money and storage. The resolution will be
} } limited by the microscope, and not by the number of
} } pixels of the camera. Likewise, if you are doing
} } Fluorescence, a camera with fewer pixels can be more
} } sensitive and thus be superior to a camera with gobs
} } of pixels.
} }
} } Also, please explain your comment that "all cameras
} } ""see"" things as an 18% grey card".
} }
} } mike
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 12596 West Bayaud Avenue
} } Suite 300
} } Lakewood, CO 80228
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} } -----Original Message-----
} } X-from: hoffpajo-at-yahoo.com
} } [mailto:hoffpajo-at-yahoo.com]
} } Sent: Wednesday, August 17, 2005 9:28 AM
} } To: Mike Bode
} } Subject: [Microscopy] viaWWW: LM Digital Camera
} } Recommendations?
} }
} }
} }
} }
} }
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America To
} } Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
} ----------------------------------------------------------------------------
} }
} }
} } for high quality images everything depends on the
} } pixels. the more pixels the better the image.
} } as for exposeures, everyone needs to remember all
} } cameras ""see"" things as an 18% grey card. so all
} } images need to be adjusted.
} } and yes to all those of you about to jump on me i
} } know this is simplistic.
} } --- edelmare-at-muohio.edu wrote:
} }
} } }
} } }
} } }
} } }
} }
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of
} } } America To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } }
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
} ----------------------------------------------------------------------------
} } }
} } } For years we have been using a Kodak DCS760
} } (actually a Nikon F5 SLR
} } } body with Kodak CDD and electronics).
} } } The SLR's
} } } are very nice because (1) they mount on the photo
} } ports correctly
} } } (i.e. F-mount, and otehrs), (2) they use the
} } microscopes optics alone,
} } } (3) and the SLR's have the better end
} } } (consumer/prosumer)
} } } CCD's and electronics. Negative side of things is
} } that unlike true
} } } scieitific cameras the exposure systems are not
} } setup to handle Light
} } } Microsopy type imaging very well and so a little
} } fiddling is required.
} } }
} } } We just got a new Nikon D-50 (very similar to the
} } D-70, CCD noise
} } } seems a little better, and $100 lower cost).
} } } With the Nikon
} } } Capture software it can be used in "tethered" mode
} } and driven
} } } completely by the computer. Now, when I say we
} } just got, I really
} } } mean that, the box arrived this morning and we
} } haven't installed it
} } } yet. Give me a couple of days and I will give a
} } report back.
} } }
} } } As for breaking the bank: Nikon D-50 body $720,
} } Capture software
} } } (needed for tethered operation) $100, and right
} } angle viewfinder
} } } $185.
} } }
} } } Right angle view finder: Very VERY helpful for
} } upright microscopes,
} } } but may not be needed / useful if you can use the
} } front camera port on
} } } an inverted scope.
} } }
} } } More info on the Nikon & other digital camers take
} } a look at:
} } }
} } } http://www.dpreview.com/reviews/specs/Nikon/
} } }
} } }
} } } (BTW: I do not sell cameras or anything else nor
} } have any financial
} } } interests in digital cameras or Nikon)


==============================Original Headers==============================
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From: hoffpajo-at-yahoo.com
Date: Wed, 17 Aug 2005 12:06:16 -0500
Subject: [Microscopy] Re: viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

well i think the money spent on a camera that will
grow with the applications at a reasonable price is
the way to go. the nikon will allow you to adjust the
exposure and quality of the final image. much also
depends on how much enlargement you need before you
begin to see the pixels.
i firmly believe that digital is the wave of the
future. you can get a nikon d-70 for under $1000
these days. and if you wish it is vesatal enough to be
used for macro photagraphy and making slides for
presentations.
again i have no vested interest in nikon. so hold the
flames on that issue please
john

--- Mike.Bode-at-soft-imaging.net wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
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}
} 200 nm is proabaly a bit optimistic, but let's go
} with that value. If you use a 100x lens, the 200 nm
} spot will be enlarged to 20 microns. If you use an 8
} Mpixel camera with a pixel size of 2 microns or so,
} you are oversampling by a large factor.
}
} Now, there are compelling reasons to buy an 8 Mpixel
} camera, but the number of pixels is not the only
} parameter you should use to decide on a camera.
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone:  (888) FIND SIS
}         (303) 234-9270
} fax:    (303) 234-9271
} email:  mailto:info-at-soft-imaging.com
} web:    http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: john hoffpauir [mailto:hoffpajo-at-yahoo.com]
} Sent: Wednesday, August 17, 2005 10:30 AM
} To: Mike Bode; microscopy-at-microscopy.com
} Subject: Re: [Microscopy] RE: viaWWW: LM Digital
} Camera Recommendations?
}
} again unless i am out of it isn't the highest
} resolution possible to be about 200nm in a light
} microscope? and pixel resolution to be between 3 to
} 8um, i could be wrong. again very simplistic. so i
} guess i should hang onto my D-70.
} --- Mike.Bode-at-soft-imaging.net wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of
} } America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------------
} }
} } I think this post is too simplistic. Don't play
} the Pixel game when
} } you buy a scientific camera. For
} } example: If you buy an 8 Mpixel camera to work at
} high magnification
} } on your microscope, you are wasting money and
} storage. The resolution
} } will be limited by the microscope, and not by the
} number of pixels of
} } the camera. Likewise, if you are doing
} Fluorescence, a camera with
} } fewer pixels can be more sensitive and thus be
} superior to a camera
} } with gobs of pixels.
} }
} } Also, please explain your comment that "all
} cameras ""see"" things as
} } an 18% grey card".
} }
} } mike
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 12596 West Bayaud Avenue
} } Suite 300
} } Lakewood, CO 80228
} } ===================================
} } phone:  (888) FIND SIS
} }         (303) 234-9270
} } fax:    (303) 234-9271
} } email:  mailto:info-at-soft-imaging.com
} } web:    http://www.soft-imaging.com
} } ===================================
} }
} }
} } -----Original Message-----
} } X-from: hoffpajo-at-yahoo.com
} } [mailto:hoffpajo-at-yahoo.com]
} } Sent: Wednesday, August 17, 2005 9:28 AM
} } To: Mike Bode
} } Subject: [Microscopy] viaWWW: LM Digital Camera
} Recommendations?
} }
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America To
} } Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------------
} }
} }
} } for high quality images everything depends on the
} } pixels. the more pixels the better the image.
} } as for exposeures, everyone needs to remember all
} } cameras ""see"" things as an 18% grey card. so all
} } images need to be adjusted.
} } and yes to all those of you about to jump on me i
} } know this is simplistic.
} } --- edelmare-at-muohio.edu wrote:
} }
} } }
} } }
} } }
} } }
} }
}
----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of
} } } America To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
}
----------------------------------------------------------------------------
} } }
} } } For years we have been using a Kodak DCS760
} } (actually a Nikon F5 SLR
} } } body with Kodak CDD and electronics).
} } } The SLR's
} } } are very nice because (1) they mount on the
} photo
} } ports correctly
} } } (i.e. F-mount, and otehrs), (2) they use the
} } microscopes optics alone,
} } } (3) and the SLR's have the better end
} } } (consumer/prosumer)
} } } CCD's and electronics. Negative side of things
} is
} } that unlike true
} } } scieitific cameras the exposure systems are not
} } setup to handle Light
} } } Microsopy type imaging very well and so a little
} } fiddling is required.
} } }
} } } We just got a new Nikon D-50 (very similar to
} the
} } D-70, CCD noise
} } } seems a little better, and $100 lower cost).
} } } With the Nikon
} } } Capture software it can be used in "tethered"
} mode
} } and driven
} } } completely by the computer. Now, when I say we
} } just got, I really
} } } mean that, the box arrived this morning and we
} } haven't installed it
} } } yet. Give me a couple of days and I will give
} a
} } report back.
} } }
} } } As for breaking the bank: Nikon D-50 body
} $720,
} } Capture software
} } } (needed for tethered operation) $100, and right
} } angle viewfinder
} } } $185.
} } }
} } } Right angle view finder: Very VERY helpful for
} } upright microscopes,
} } } but may not be needed / useful if you can use
} the
} } front camera port on
} } } an inverted scope.
} } }
}
=== message truncated ===




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6, 19 -- Date: Wed, 17 Aug 2005 10:06:13 -0700 (PDT)
6, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 19 -- Subject: Re: [Microscopy] viaWWW: LM Digital Camera Recommendations?
6, 19 -- To: Mike.Bode-at-soft-imaging.net, microscopy-at-microscopy.com
6, 19 -- In-Reply-To: {200508171644.j7HGiIt1007905-at-ns.microscopy.com}
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From: hoffpajo-at-yahoo.com
Date: Wed, 17 Aug 2005 12:14:23 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

yes by all means a side by side comarison is the way
to go.
i don't belive you can have to much hard disk or RAM,
or pixels. i typicaly enlarge and pics i take by quite
a bit. i use the nikon on a telescope for taking
images of the moon and other objects in daylight.
it is a hobby of mine.
john hoffpauir
adding my name here so you wont have to look for it in
the future.

--- wesaia-at-iastate.edu wrote:

}
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}
} Supposing the resolution is 200 nm, that is less
} than half a wavelength of
} light. That might be attainable given a lens (and
} other optics) of
} sufficiently high NA. Those are generally the higher
} power objectives. My
} basic 40x lens has an NA of only 0.63, so I won't
} get such good resolution.
} But suppose we could get 200 nm out of a 100x
} objective.
}
} 200 nm resolution means we would need pixels every
} 100 nm or so. I
} generally figure prints of 120 mm or so wide. That
} means the width of my
} image will be about 120 um at 1000x. That means I
} will need 1200 pixels
} across my image for a 100-nm pixel spacing. That
} isn't very many pixels in
} today's terms. My old 1.3 megapixel Pixera can
} handle that.
}
} You might say that the extra pixels allow you to
} take lower magnification
} images and still record up to the resolution limit.
} First, what is the
} resolution for that set of lenses? The NA and
} resolution falls off with a
} drop in magnification. There may not be detail to
} record with the extra
} pixels.
}
} Having said all that, 3 megapixel consumer cameras
} are practically throw
} away items now. For that matter, so are 40 GB disk
} drives. It doesn't hurt
} to add a few more pixels (and MB) to be safe.
} However, I think a lot of
} people are quick to take high-pixel images for which
} they never will use
} the information contained there. They just provide a
} demand for more and
} bigger hard drives.
}
} I am going to have to run a comparison here one of
} these days. I have a gut
} feeling that lower pixel number cameras may be more
} sensitive in low-light
} situations than their higher pixel cousins. (I think
} that is what Mike Bode
} suggested.) I want to do a side-by-side comparison
} to show myself and
} others. I will grant that higher pixel cameras can
} record more detail in
} their regular photographic mode under sufficient
} light. I just don't know
} that they have the same edge in the lab.
}
} Warren Straszheim
} (adding my name here so you don't have to check out
} the address line above
} or below)
}
} At 11:31 AM 08/17/05, John H. wrote:
}
} } again unless i am out of it isn't the highest
} } resolution possible to be about 200nm in a light
} } microscope? and pixel resolution to be between 3 to
} } 8um, i could be wrong. again very simplistic. so i
} } guess i should hang onto my D-70.
} } --- Mike.Bode-at-soft-imaging.net wrote:
} }
} } }
} } }
} } }
} } }
}
} ----------------------------------------------------------------------------
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} } }
} } } I think this post is too simplistic. Don't play
} the
} } } Pixel game when you buy a scientific camera. For
} } } example: If you buy an 8 Mpixel camera to work
} at
} } } high magnification on your microscope, you are
} } } wasting money and storage. The resolution will
} be
} } } limited by the microscope, and not by the number
} of
} } } pixels of the camera. Likewise, if you are doing
} } } Fluorescence, a camera with fewer pixels can be
} more
} } } sensitive and thus be superior to a camera with
} gobs
} } } of pixels.
} } }
} } } Also, please explain your comment that "all
} cameras
} } } ""see"" things as an 18% grey card".
} } }
} } } mike
} } }
} } } Michael Bode, Ph.D.
} } } Soft Imaging System Corp.
} } } 12596 West Bayaud Avenue
} } } Suite 300
} } } Lakewood, CO 80228
} } } ===================================
} } } phone: (888) FIND SIS
} } } (303) 234-9270
} } } fax: (303) 234-9271
} } } email: mailto:info-at-soft-imaging.com
} } } web: http://www.soft-imaging.com
} } } ===================================
} } }
} } }
} } } -----Original Message-----
} } } X-from: hoffpajo-at-yahoo.com
} } } [mailto:hoffpajo-at-yahoo.com]
} } } Sent: Wednesday, August 17, 2005 9:28 AM
} } } To: Mike Bode
} } } Subject: [Microscopy] viaWWW: LM Digital Camera
} } } Recommendations?
} } }
} } }
} } }
} } }
} } }
}
} ----------------------------------------------------------------------------
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} } } Microscopy Society of America To
} } } Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
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} } }
}
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
}
} ----------------------------------------------------------------------------
} } }
} } }
} } } for high quality images everything depends on
} the
} } } pixels. the more pixels the better the image.
} } } as for exposeures, everyone needs to remember
} all
} } } cameras ""see"" things as an 18% grey card. so
} all
} } } images need to be adjusted.
} } } and yes to all those of you about to jump on me
} i
} } } know this is simplistic.
} } } --- edelmare-at-muohio.edu wrote:
} } }
} } } }
} } } }
} } } }
} } } }
} } }
}
} ----------------------------------------------------------------------------
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} } } Microscopy Society of
} } } } America To Subscribe/Unsubscribe --
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} } }
}
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } }
}
} ----------------------------------------------------------------------------
} } } }
} } } } For years we have been using a Kodak
} DCS760
} } } (actually a Nikon F5 SLR
} } } } body with Kodak CDD and electronics).
} } } } The SLR's
} } } } are very nice because (1) they mount on the
} photo
} } } ports correctly
} } } } (i.e. F-mount, and otehrs), (2) they use the
} } } microscopes optics alone,
}
=== message truncated ===




____________________________________________________
Start your day with Yahoo! - make it your home page
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From: frah0010-at-tc.umn.edu
Date: Wed, 17 Aug 2005 13:02:02 -0500
Subject: [Microscopy] microprobe user listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What you said here certainly makes sense in many cases. Other people have different requirements. For example, they might want to have a live image on a PC screen to do more processing, or integrate the camera into an automated system, or provide the images live over the internet to a colleague. Many other applications come to mind that require a different camera than the one you are suggesting.

This only supports my initial point that the number of pixels should not be the only parameter to look at when you buy a camera for a microscope.


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: john hoffpauir [mailto:hoffpajo-at-yahoo.com]
Sent: Wednesday, August 17, 2005 11:06 AM
To: Mike Bode; microscopy-at-microscopy.com

To: electron microprobe users, technicians, and lab managers

Re: new JEOL microprobe user listserver

Colleagues,

Rather than merely wishing for the twentieth time that there was a
listserver for JEOL microprobe users, technicians, and lab managers
to share questions, knowledge, news, problems, and experience (and
even socialize a bit), I decided to actually set up one.

There has been a listserver for Cameca SX50 users since 1996 to
discuss problems, suggest improvements to Cameca, plan meetings at
national conferences, and so on. I initially considered having a
8800/8900-only listserver, but I decided that there are more
similarities than differences across the generations. Whether you
use a classic JEOL JXA-733, a new field-emission 8500, or anything in-
between, this is for you. Of course, Cameca/ARL/whatever users are
welcome too!

I know, I know -- the last thing you need is more e-mail, but a
listserver is only as strong as its subscribers. Let's make this a
valuable resource! This, of course, is not meant to replace the
excellent MSA listserver (Thanks, Nestor!), but I think that it will
be valuable to more targeted list at our disposal, rather than e-
mailing all 3000 MSA subscribers. The same goes for the MAS
listserver too.

This is a moderated listserver, meaning all subscribers and all posts
are approved by a moderator (me). Moderation is intented to
eliminate spam and other junk mail from being sent to all subscribers.

There is a webpage with details here: http://probelab.geo.umn.edu/
listserver.html.

To subscribe, e-mail the message "SUB PROBEUSERS {YOUR_NAME} " to the
address: listserv-at-lists.umn.edu.

Please e-mail me with any subscription problems -- I want everyone to
be able to participate! Also please forward this to colleagues who
might be interested and forgive any multiple copies -- I'm trying to
announce this widely.

Best,
Ellery

--------------------
Ellery E. Frahm
Research Scientist/Manager
Electron Microprobe Laboratory
University of Minnesota - Twin Cities
Department of Geology & Geophysics
Lab Website: http://probelab.geo.umn.edu


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From: beth-at-plantbio.uga.edu
Date: Wed, 17 Aug 2005 14:10:16 -0500
Subject: [Microscopy] teaching scopes & digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
In the same vein as the LM digital discussion my department was to
equip all the LM scopes in the introductory botany class with digital
cameras. They also want to be able to project what is being viewed on
the instructor's scope and have the students to be able to project what
is being viewed on their scopes (to share with the whole class).
Has anyone done this set up for their department? Or, know if there is
a company out there that would set up the lab for us?
Any help/suggestions would be greatly appreciated...especially from
vendors.
thanks,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



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From: walck-at-southbaytech.com
Date: Wed, 17 Aug 2005 15:42:05 -0500
Subject: [Microscopy] Re: TEM Sample Prep: Plasma Cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to add a few statements to the discussion and support some
of the statements that Nestor made with his posting.

We had a demonstration at the M&M 2005 in our booth where we dipped
various samples into mechanical pump oil (hydrocarbon based), wiped them
with a cloth, estimated the contact angle, plasma cleaned them with our
PC2000 using just atmospheric air for 5 min, and then took them out, and
the samples were ultrahydrophilic (contact angle less than 5 degrees).
Of course prior to doing this at the show, we did it in our lab. We did
this demonstration to put a stake through the heart of any arguments
concerning an oil vs an oiless pumping system. What we did not make
public at the show were results from our studies that showed that using
Ar on the samples also had a significant cleaning affect. (We also did
not show the results for Fomblin(R) and silicone based oils that showed
the same results as the hydrocarbon oil.) Five to ten minutes with
about the same power as was used for air gave clean surfaces. These are
the similar parameters that Nestor is discussing below in his posting
and our results are in complete agreement with his findings. We did
find a difference with different materials, (semiconductor, ceramic, and
metal), but if cleaned long enough (maximum about 10 minutes) all of the
samples exhibited the cleaned surface void of hydrocarbons as evidenced
by the contact angle.

I also have anecdotal information concerning plasma cleaning of glass
TEM samples that were coated with carbon. I had samples that I ion
sputtered graphite and other samples that I evaporated carbon onto.
These samples were then plasma cleaned (in a competitor's plasma
cleaner). It has been awhile since I did this, but one form (I think
that it was the evaporated carbon) resisted plasma cleaning while carbon
from the ion sputtered samples was removed. I went almost 20 minutes
without the carbon being removed. This strongly suggests that the form
of carbon is very important as Nestor suggests. To my knowledge, nobody
has looked into this. I know that the amount of hydrogen in
diamond-like carbon (DLC) films does change the Raman spectra from these
films. Raman or FTIR techniques might be interesting method to use in
an attempt to relate to how different forms of carbon perform using
oxygen plasmas. I know that DLC films with differing levels of hydrogen
that are grown for tribological applications have different properties
in a humid environments. The more hydrogen in the films, the more they
are susceptible to failure in a humid atmosphere.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov]
Sent: Wednesday, August 17, 2005 9:06 AM
To: Walck-at-SouthBayTech.com

Roy

We routinely plasma clean holey/continuous carbon films with
particles on them it works
fine here at ANL. I've only done a few (i.e. { 5) microtomed
samples, but have not had
any problems with them, but that is not a significant number of
samples to gauge the
effectiveness.

The key here is to recognize, as you have apparently already done, that
the reactive plasma obviously will attack the carbon in your support as
well as the source of hydrocarbon contamination. I have found that the
hydrocarbon, not surprizingly is more reactive than, for
example, the more
stable carbon support films. The key here is to tailor your gas
composition
as well as to adjust the power levels of your plasma. At ANL, for
carbon support films, we
use Argon gas only and at a low power setting of ~ 5-10 W for ~ 10
minutes in our SBT PC-2000 plasma cleaner. In my experience, for these
type of samples, you should keep oxygen to a minimum as this will
rapidly attach all
carbon. Basically,
we have found that there will be enough residual oxygen released by
the Argon plasma
to attack the organic contamination, assuming the specimen is not
heavily coated.

Of course, not all "carbon support films", are created equal and
some have more
hydrocarbon than others, so you will need to test the receipe for your
films as well as for the model of plasma cleaner and its settings as
each manufactureres unit will vary in efficacy. It is best here to make
a few sacrifical films and tune your settings to minimze the reaction
on your support films.

Nestor
Your Friendly Neighborhood SysOp

Disclaimer: My employer Argonne National Lab holds the basic patent
on TEM/SEM plasma
cleaning technology and licenses this to manufacturers of commerical
units.


} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America

} Our specific samples are inorganic (mineral plus some carbonaceous
} material) grains in epoxy ultramicrotomy sections suppoted on
} continuous carbon film TEM grids. We would like to know whether
} contamination can be successfully prevented, or removed, from these
} samples by plasma cleaning (while in the TEM holder) without also
} causing destruction, alteration or volatilization of the epoxy section
} and/or carbon support film.
}
}
}
} Thanks,
}
}
}
} Roy Christoffersen
}
} SAIC
}
} NASA Johnson Space Center EM Facilities
}


--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

===========================================

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From: Mike.Bode-at-soft-imaging.net
Date: Wed, 17 Aug 2005 16:00:08 -0500
Subject: [Microscopy] teaching scopes & digital cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Beth,

If you have a setup that allows sharing of a LCD projector between different PCs, all you would need is a camera that provides a live image on the PC screen. Student's PCs as well as the instructor's PC are connected to the projector. You would then simply switch between the PC to show what is on their screen.

Before you buy all that equipment, make sure that the quality of the projected image is sufficient. LCD projectors are very good for presentations, but the nuances of microscopic images might get lost.

Alternately you could use something like "remote desktop" to connect the instructor's PC to another PC in the classroom. The instructor's PC would then show the students desktop and be displayed via the projector. That way you would not have to deal with multiple connections to the projector, but the setup may be more complicated. Again, check the quality first. "remote desktop" will reduce colors if the bandwidth is too low, and you will get a bad image displayed on the screen.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu]
Sent: Wednesday, August 17, 2005 1:13 PM
To: Mike Bode

Hi all,
In the same vein as the LM digital discussion my department was to equip all the LM scopes in the introductory botany class with digital cameras. They also want to be able to project what is being viewed on the instructor's scope and have the students to be able to project what is being viewed on their scopes (to share with the whole class).
Has anyone done this set up for their department? Or, know if there is a company out there that would set up the lab for us?
Any help/suggestions would be greatly appreciated...especially from vendors.
thanks,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805 http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



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From: DusevichV-at-umkc.edu
Date: Wed, 17 Aug 2005 17:27:13 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} I am going to have to run a comparison here one of these
} days. I have a gut
} feeling that lower pixel number cameras may be more sensitive
} in low-light
} situations than their higher pixel cousins. (I think that is
} what Mike Bode
} suggested.)

Lower pixel number means lower noise at low signal level.


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy


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From: jhjia-at-uwm.edu
Date: Wed, 17 Aug 2005 18:39:53 -0500
Subject: [Microscopy] viaWWW: Ulruamicrotomy of carbon nanotubes in Poly(vinyl alcohol)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jhjia-at-uwm.edu) from http://www.microscopy.com/MLFormMail.html on Wednesday, August 17, 2005 at 09:28:04
---------------------------------------------------------------------------

Email: jhjia-at-uwm.edu
Name: Junhong JIA

Organization: University of Wisconsin- Milwaukee

Title-Subject: [Filtered] MListserver:Ulruamicrotomy of carbon nanotubes in Poly(vinyl alcohol)

Question: Dear Sirs:

Is there any fluid that can be used in a knife boat for cutting utrathin sections from a specimen consitins of carbon nanotubes in poly(vinyl alcohol)?

Any helpful inforation should be highly appriciated.

Dr. Junhong JIA
Department of Mechanical Engineering
University of Wisconsin- Milwaukee
Milwaukee, WI 53211
Phone:414-229-2498


---------------------------------------------------------------------------

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From: mkomboli-at-uno.edu
Date: Wed, 17 Aug 2005 18:40:19 -0500
Subject: [Microscopy] viaWWW: LR gold -Thanks

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mkomboli-at-uno.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 17, 2005 at 11:38:42
---------------------------------------------------------------------------

Email: mkomboli-at-uno.edu
Name: Mary Kombolias

Organization: University of New Orleans

Title-Subject: [Filtered] LR gold

Question: Thanks to everybody who replied to my question! I appreciate the help. Peace out!

---------------------------------------------------------------------------

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From: walck-at-southbaytech.com
Date: Wed, 17 Aug 2005 20:39:24 -0500
Subject: [Microscopy] TEM Sample Prep: Plasma Cleaning Ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is more that I would like to add concerning plasma cleaners
affecting carbon films.

In the paper, "Surface Science Aspects of Contamination in TEM Sample
Preparation, J. T. Grant, S. D. Walck, F. J. Scheltens, A. A. Voevodin,
Proceedings of the Materials Research Society, Workshop on Specimen
Preparation for Transmission Electron Microscopy of Materials IV, eds.
Ron M. Anderson and Scott D. Walck, Pittsburgh, Vol 480, (1997), there
was a difference in the ability of the two types of plasma cleaners,
capacitively coupled and inductively coupled in the removal rate of
existing electron beam induced contamination. At the time, I thought
that this could be due to the difference in plasma densities between the
two systems. However, subsequent experiments at SBT showed that
pre-existing contamination could be removed by increasing the power and
lowering the pressure in the capacitively coupled system. (BTW, that's
SBT's design.) What these parameter changes do is increase the energies
of species in the plasma. In retrospect, the inductively coupled system
operated at a lower pressure than the capacitively coupled system and I
now believe that it is a difference in the energies of the species in
the plasma between the two systems that caused the difference. With
respect to the current topic question of how a plasma cleaner will
affect a carbon containing film, these parameters give another degree of
operator control in how the carbon containing materials are affected.

Incidentally, a recent flyer from a vender at the M&M 2005 meeting
claimed that an addition of hydrogen to the O2 plasma worked better and
that this was a discovery of theirs. It is well known in the literature
that an addition of H2 to a N2 or an O2 plasma will increase the
activated species of nitrogen and oxygen and in the case of oxygen are
the ones that are responsible for the cleaning. In fact, additions of
He will also increase the activated species and you don't have the
potential hazard. This is used in reactive sputter deposition systems
to increase the stoichiometry of oxide and nitride films. However, a
trade-off is that a light-element only plasma will increase the ion
temperature which could possible have adverse affects. The addition of
Ar which has a higher mass helps cool the plasma temperature. However,
keeping the results of Nestor's temperature rise experiments in mind,
I'm not sure how important this affect could be, but for
carbon-containing films, it could be very important.

I probably should give a disclaimer to the effect that SBT makes and
sells a plasma cleaner, but the above paragraphs are too "sciencey" as
opposed to "salesy", so I won't! OOPs, I guess I just did.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: rcsaic-at-sbcglobal.net [mailto:rcsaic-at-sbcglobal.net]
Sent: Wednesday, August 17, 2005 7:47 AM
To: Walck-at-SouthBayTech.com

We are interested in feedback from any group in the community who has
experience with plasma cleaning TEM samples prepared by ultramicrotomy.
Our specific samples are inorganic (mineral plus some carbonaceous
material) grains in epoxy ultramicrotomy sections suppoted on continuous
carbon film TEM grids. We would like to know whether contamination can
be successfully prevented, or removed, from these samples by plasma
cleaning (while in the TEM holder) without also causing destruction,
alteration or volatilization of the epoxy section and/or carbon support
film.



Thanks,



Roy Christoffersen

SAIC

NASA Johnson Space Center EM Facilities


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From: David.Patton-at-uwe.ac.uk
Date: Thu, 18 Aug 2005 04:54:11 -0500
Subject: [Microscopy] Questions on sodium azide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I could do with some help with my risk assessment. This stuff seems to
be fascinatingly reactive yet I know many labs use it for storing
biological samples. I am planning to store samples in 0.02% sodium
azide and sodium cacodylate for 3-4 months. On contact with water
sodium azide produces a toxic gas. One safety site said it should
therefore only be stored in a fume hood. Another site stated that it
should not be stored in fridges with exposed copper or lead parts. My
sparkproof fridge suppliers advised against it. I am dubious about
storing biological samples at room temperature. Any suggestions?



Dave



This email has been independently scanned for viruses and any virus software has been removed using McAfee anti-virus software


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From: jbs-at-temple.edu
Date: Thu, 18 Aug 2005 11:05:09 -0500
Subject: [Microscopy] Re: viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been following this discussion on pixel number and resolution
for LM cameras with great interest.

It occurs to me that it might be useful to have a discussion of bit
depth as well. I realize that most cameras are standardized at 24
bits, which is really 8 bits per color channel. Although this does
give quite a range of color values, the intensity range is limited to
256 gray steps. I wonder if there is a need for greater range, so
that we can make finer disctinctions among intensities and/or record
a greater range, from bright to dim.

Joel


}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
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}
} } I am going to have to run a comparison here one of these
} } days. I have a gut
} } feeling that lower pixel number cameras may be more sensitive
} } in low-light
} } situations than their higher pixel cousins. (I think that is
} } what Mike Bode
} } suggested.)
}
} Lower pixel number means lower noise at low signal level.
}
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 3127 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
}
} ==============================Original
} Headers============================== 6, 23 -- From DusevichV-at-umkc.edu
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} Recommendations? 6, 23 -- Date: Wed, 17 Aug 2005 17:27:11 -0500 6, 23
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Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


==============================Original Headers==============================
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From: cammer-at-aecom.yu.edu
Date: Thu, 18 Aug 2005 11:15:08 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I highly recommend http://www.photomet.com/library_encyclopedia.shtml


At 11:08 AM 08/18/05 -0500, you wrote:


} I have been following this discussion on pixel number and resolution
} for LM cameras with great interest.
}
} It occurs to me that it might be useful to have a discussion of bit
} depth as well. I realize that most cameras are standardized at 24
} bits, which is really 8 bits per color channel. Although this does
} give quite a range of color values, the intensity range is limited to
} 256 gray steps. I wonder if there is a need for greater range, so
} that we can make finer disctinctions among intensities and/or record
} a greater range, from bright to dim.
}
} Joel

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**


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7, 24 -- Subject: Re: [Microscopy] Re: viaWWW: LM Digital Camera
7, 24 -- Recommendations?
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From: xin-at-magnet.fsu.edu
Date: Thu, 18 Aug 2005 11:34:55 -0500
Subject: [Microscopy] Postdoctoral Position in Scanning Transmission Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Postdoctoral Position in Scanning Transmission Electron Microscopy -
University of California, Santa Barbara

Applicants must have extensive and demonstrated experience in several areas
of TEM and a strong background in materials science and diffraction.
Preference will be given to applicants with expertise in STEM techniques,
such as atomic resolution HAADF imaging. Facilities at UCSB include a
Tecnai F30U TEM/STEM and other state-of-the-art imaging, spectroscopy and
diffraction facilities in the UCSB MRL. Research projects include the
characterization of high-permittivity oxide thin films, including gate
dielectrics and ferroelectrics.

The position is available in early 2006. Duration about 1-2 years, salary
is commensurate with
qualifications. Candidates must have a Ph.D. in Materials Science or
Physics. Interested candidates should send a curriculum vitae, publication
list and the names of at least three references with their contact
information to:

Prof. Susanne Stemmer
Materials Department
University of California
Santa Barbara, CA 93106-5050
Email: stemmer-at-mrl.ucsb.edu
http://www.mrl.ucsb.edu/~stemmer
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


==============================Original Headers==============================
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5, 24 -- Subject: Postdoctoral Position in Scanning Transmission Electron
5, 24 -- Microscopy - University of California, Santa Barbara
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From: jbs-at-temple.edu
Date: Thu, 18 Aug 2005 11:52:55 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks Michael. It is a useful resource.

It seems to the me that the real determinant of how much need there
is for increased bit depth is a function of the images. Many of the
fluorescence images I have seen appear to be saturated, in which case
perhaps 2 or four bits would be sufficient. However, if one is
interested in FRET or other quantitative techniques, the bit depth
may matter. As with pixel density, bit depth depends on the samples.




} I highly recommend http://www.photomet.com/library_encyclopedia.shtml
}
}
}
}
} At 11:08 AM 08/18/05 -0500, you wrote:
}
}
}
} } ---------------------------------------------------------------------
} } ------- The Microscopy ListServer -- CoSponsor: The Microscopy
} } Society of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ---------------------------------------------------------------------
} } -------
} }
} } I have been following this discussion on pixel number and resolution
} } for LM cameras with great interest.
} }
} } It occurs to me that it might be useful to have a discussion of bit
} } depth as well. I realize that most cameras are standardized at 24
} } bits, which is really 8 bits per color channel. Although this does
} } give quite a range of color values, the intensity range is limited to
} } 256 gray steps. I wonder if there is a need for greater range, so
} } that we can make finer disctinctions among intensities and/or record
} } a greater range, from bright to dim.
} }
} } Joel
} }
} }
} } }
} } }
} } }
} } } ------------------------------------------------------------------
} } } ---- ------ The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver On-Line Help
} } } http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ------------------------------------------------------------------
} } } ---- ------
} } }
} } } } I am going to have to run a comparison here one of these
} } } } days. I have a gut
} } } } feeling that lower pixel number cameras may be more sensitive in
} } } } low-light situations than their higher pixel cousins. (I think
} } } } that is what Mike Bode suggested.)
} } }
} } } Lower pixel number means lower noise at low signal level.
} } }
} } }
} } } Vladimir M. Dusevich, Ph.D.
} } } Electron Microscope Lab Manager
} } } 3127 School of Dentistry
} } } 650 E. 25th Street
} } } Kansas City, MO 64108-2784
} } }
} } } Phone: (816) 235-2072
} } } Fax: (816) 235-5524
} } } Web: http://www.umkc.edu/dentistry/microscopy
} } }
} } }
} } } ==============================Original
} } } Headers============================== 6, 23 -- From
} } } DusevichV-at-umkc.edu Wed Aug 17 17:27:13 2005 6, 23 -- Received:
} } } from kc-msxproto3.kc.umkc.edu (imap4.exchange.umkc.edu
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} } } Subject: RE: [Microscopy] Re: viaWWW: LM Digital Camera
} } } Recommendations? 6, 23 -- Date: Wed, 17 Aug 2005 17:27:11 -0500 6,
} } } 23 -- Message-ID:
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} } } Recommendations? 6, 23 -- Thread-Index:
} } } AcWjTe7nuT+e71apTjeoVuPp2YSpdQALDjBQ 6, 23 -- From: "Dusevich,
} } } Vladimir" {DusevichV-at-umkc.edu} 6, 23 -- To:
} } } {microscopy-at-microscopy.com} 6, 23 -- X-OriginalArrivalTime: 17 Aug
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} }
} }
} } Joel B. Sheffield, Ph.D.
} } Biology Department, Temple University
} } 1900 North 12th Street
} } Philadelphia, PA 19122
} } jbs-at-temple.edu
} } (215) 204 8839, fax (215) 204 0486
} } http://astro.temple.edu/~jbs
} }
} }
} } ==============================Original
} } Headers============================== 8, 20 -- From jbs-at-temple.edu
} } Thu Aug 18 11:05:08 2005 8, 20 -- Received: from imp1.temple.edu
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} } 20 -- for {microscopy-at-microscopy.com} ; Thu, 18 Aug 2005
} } 12:05:09 -0400 8, 20 -- From: "Joel Sheffield" {jbs-at-temple.edu} 8, 20
} } -- To: microscopy-at-microscopy.com 8, 20 -- Date: Thu, 18 Aug 2005
} } 12:05:08 -0400 8, 20 -- MIME-Version: 1.0 8, 20 -- Subject: Re:
} } [Microscopy] viaWWW: LM Digital Camera Recommendations? 8, 20 --
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}
} ______________________________________________________________________
} ______ Michael Cammer Analytical Imaging Facility Albert Einstein
} Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave.
} Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL:
} http://www.aecom.yu.edu/aif/
} **This electronic transmission contains information that is
} privileged.**
}


Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


==============================Original Headers==============================
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From: Mike.Bode-at-soft-imaging.net
Date: Thu, 18 Aug 2005 13:07:47 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You are absolutely right that even a 24-bit image can only show 256 levels of gray. This compares to about 60 or so that the human eye can distinguish under optimum circumstances. Also, as far as I know, there are no monitors out there that can display more than 8 bit per color, so for displaying the images, this range is probably sufficient.

However, most people want to do further processing of the images, or they need a larger range for acquiring the images (diffraction patterns come to mind), and in fact, most cameras can provide images that have a wider range. Just as an example, our Cantega (a TEM b/w camera), can provide 16 bit of gray level information, or 65K gray levels. These images can then be stored as 16-bit gray level images to keep all the information contained in the image. The question then turns to how you can display this on an 8-bit monitor, but there are solutions to that. Other cameras have 14 , 12 or 10 bit ranges, and they are typically also stored as 16-bit files. For color, there is a 48-bit format that can be used. The files are getting pretty large then. If you have a 5 Mpixel color camera and store the image as a 48-bit file, each image requires 30 MB of storage.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: cammer-at-aecom.yu.edu [mailto:cammer-at-aecom.yu.edu]
Sent: Thursday, August 18, 2005 10:17 AM
To: Mike Bode

I highly recommend http://www.photomet.com/library_encyclopedia.shtml


At 11:08 AM 08/18/05 -0500, you wrote:


} I have been following this discussion on pixel number and resolution
} for LM cameras with great interest.
}
} It occurs to me that it might be useful to have a discussion of bit
} depth as well. I realize that most cameras are standardized at 24
} bits, which is really 8 bits per color channel. Although this does
} give quite a range of color values, the intensity range is limited to
} 256 gray steps. I wonder if there is a need for greater range, so that
} we can make finer disctinctions among intensities and/or record a
} greater range, from bright to dim.
}
} Joel

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**


==============================Original Headers==============================
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==============================Original Headers==============================
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From: xin-at-magnet.fsu.edu
Date: Thu, 18 Aug 2005 16:29:42 -0500
Subject: [Microscopy] Postdoctoral Position in Scanning Transmission Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Postdoctoral Position in Scanning Transmission Electron Microscopy -
University of California, Santa Barbara

Applicants must have extensive and demonstrated experience in several areas
of TEM and a strong background in materials science and diffraction.
Preference will be given to applicants with expertise in STEM techniques,
such as atomic resolution HAADF imaging. Facilities at UCSB include a
Tecnai F30U TEM/STEM and other state-of-the-art imaging, spectroscopy and
diffraction facilities in the UCSB MRL. Research projects include the
characterization of high-permittivity oxide thin films, including gate
dielectrics and ferroelectrics.

The position is available in early 2006. Duration about 1-2 years, salary
is commensurate with
qualifications. Candidates must have a Ph.D. in Materials Science or
Physics. Interested candidates should send a curriculum vitae, publication
list and the names of at least three references with their contact
information to:

Prof. Susanne Stemmer
Materials Department
University of California
Santa Barbara, CA 93106-5050
Email: stemmer-at-mrl.ucsb.edu
http://www.mrl.ucsb.edu/~stemmer


==============================Original Headers==============================
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5, 24 -- To: microscopy-at-microscopy.com
5, 24 -- From: Yan Xin {xin-at-magnet.fsu.edu}
5, 24 -- Subject: Postdoctoral Position in Scanning Transmission Electron
5, 24 -- Microscopy - University of California, Santa Barbara
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From: Stacey.Andringa-at-uc.edu
Date: Thu, 18 Aug 2005 17:28:23 -0500
Subject: [Microscopy] viaWWW: spent photographic fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Thursday, August 18, 2005 at 09:57:24
---------------------------------------------------------------------------

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: I have been catching up on some lab cleaning and have about 12 gallons of spent photographic fixer. I have been told that I can sink dispose of this using copious amounts of water or pay to have our chemical disposal contractor pick it up and dispose of it. Does anyone have a simple on-site silver recovery system I could try first? Is it worth the trouble/expense?
Thanks for any help.

Stacey Andringa


---------------------------------------------------------------------------

==============================Original Headers==============================
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8, 12 -- Subject: viaWWW: spent photographic fixer
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: neuberger1234-at-comcast.net
Date: Thu, 18 Aug 2005 22:02:00 -0500
Subject: [Microscopy] viaWWW: LM Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike,

As you imply, the higher bit depth in B&W as well as color might be useful
performing image analysis where display of the image is not necessary but
obtaining data and statistics is. Re file size, the Nikon D2X raw image
opened in Image Capture and then converted to a TIFF file in Photoshop is
70MB! Takes a bit of processing power, that one does; think I'm going to
have to go to a dual processor system.

Damian Neuberger, Ph.D.


You are absolutely right that even a 24-bit image can only show 256 levels
of gray. This compares to about 60 or so that the human eye can distinguish
under optimum circumstances. Also, as far as I know, there are no monitors
out there that can display more than 8 bit per color, so for displaying the
images, this range is probably sufficient.

However, most people want to do further processing of the images, or they
need a larger range for acquiring the images (diffraction patterns come to
mind), and in fact, most cameras can provide images that have a wider range.
Just as an example, our Cantega (a TEM b/w camera), can provide 16 bit of
gray level information, or 65K gray levels. These images can then be stored
as 16-bit gray level images to keep all the information contained in the
image. The question then turns to how you can display this on an 8-bit
monitor, but there are solutions to that. Other cameras have 14 , 12 or 10
bit ranges, and they are typically also stored as 16-bit files. For color,
there is a 48-bit format that can be used. The files are getting pretty
large then. If you have a 5 Mpixel color camera and store the image as a
48-bit file, each image requires 30 MB of storage.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228



==============================Original Headers==============================
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11, 23 -- Cc: "Microscopy-at-Microscopy. Com" {microscopy-at-microscopy.com}
11, 23 -- Subject: RE: [Microscopy] RE: viaWWW: LM Digital Camera
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From: jroszka-at-beaumont.edu
Date: Fri, 19 Aug 2005 07:29:44 -0500
Subject: [Microscopy] viaWWW: Looking into Imaging Plate Technology for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jroszka-at-beaumont.edu) from http://www.microscopy.com/MLFormMail.html on Friday, August 19, 2005 at 07:20:30
---------------------------------------------------------------------------

Email: jroszka-at-beaumont.edu
Name: jroszka

Organization: w.beaumonthospital

Title-Subject: [Filtered] MListserver:

Question: Looking into the DITABIS Imaging Plate Technology for TEM photography. Anyone with practical expierence or insight with this system (this system would be applied to a Philips 201 TEM) ? Thanks

---------------------------------------------------------------------------

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From: dsoren-at-umich.edu
Date: Fri, 19 Aug 2005 07:51:41 -0500
Subject: [Microscopy] TEM tissue processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

We're considering a tissue processor for EM samples. We would like
to hear from any of you that have thoughts about the Leica EM TP
Tissue Processor or the EMS LYNX Tissue Processor. The usual types
of questions like do they work well, how is your support, price, etc.
or any other information that you may think is important would be
very useful. Thank you.

Dotty Sorenson
Microscopy and Image-analysis Laboratory
University of Michigan

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From: mcauliff-at-umdnj.edu
Date: Fri, 19 Aug 2005 11:59:45 -0500
Subject: [Microscopy] Re: viaWWW: spent photographic fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here in New Jersey we are required to dispose of used fixer as hazardous
waste, due to the silver content. Kodak sells (or used to sell) a silver
recovery kit. so you might try them.

Geoff

Stacey.Andringa-at-uc.edu wrote:

}
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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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8, 32 -- Date: Fri, 19 Aug 2005 13:00:14 -0400
8, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
8, 32 -- Subject: Re: [Microscopy] viaWWW: spent photographic fixer
8, 32 -- In-reply-to: {200508182230.j7IMULQ8009016-at-ns.microscopy.com}
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From: hoffpajo-at-yahoo.com
Date: Fri, 19 Aug 2005 14:53:14 -0500
Subject: [Microscopy] viaWWW: spent photographic fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

i remember those days when i worked in NJ, it was a
pain in the but to collect all the spent fixer. you
are right about kodax and the silver recovery kit.
someone else used to do it as well but alas that brain
cell is long gone.
i do believe if you truly just want the silver in it's
raw form you can use steel wool so the silver will
react with the steel wool and bind to it. perhaps some
on the list server can explain the chemistry to us
all. i was never any good at inorganic chemisrty.
john hoffpauir



--- mcauliff-at-umdnj.edu wrote:

}
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}
}
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}
} Here in New Jersey we are required to dispose of
} used fixer as hazardous
} waste, due to the silver content. Kodak sells (or
} used to sell) a silver
} recovery kit. so you might try them.
}
} Geoff
}
} Stacey.Andringa-at-uc.edu wrote:
}
} }
}
} ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
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} } On-Line Help
}
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} ----------------------------------------------------------------------------
} }
} } Below is the result of your feedback form
} (NJZFM-ultra-55). It was submitted by
} (Stacey.Andringa-at-uc.edu) from
} http://www.microscopy.com/MLFormMail.html on
} Thursday, August 18, 2005 at 09:57:24
}
} ---------------------------------------------------------------------------
} }
} } Email: Stacey.Andringa-at-uc.edu
} } Name: Stacey Andringa
} }
} } Organization: University of Cincinnati
} }
} } Title-Subject: [Filtered] MListserver:
} }
} } Question: I have been catching up on some lab
} cleaning and have about 12 gallons of spent
} photographic fixer. I have been told that I can sink
} dispose of this using copious amounts of water or
} pay to have our chemical disposal contractor pick it
} up and dispose of it. Does anyone have a simple
} on-site silver recovery system I could try first? Is
} it worth the trouble/expense?
} } Thanks for any help.
} }
} } Stacey Andringa
} }
} }
}
} ---------------------------------------------------------------------------
} }
} } ==============================Original
} Headers==============================
} } 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 18
} 17:28:23 2005
} } 8, 12 -- Received: from [206.69.208.22]
} (mac22.zaluzec.com [206.69.208.22])
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} with ESMTP id j7IMSLGo006890
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} Aug 2005 17:28:22 -0500
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} } 8, 12 -- To: microscopy-at-microscopy.com
} } 8, 12 -- From: Stacey.Andringa-at-uc.edu (by way of
} MicroscopyListserver)
} } 8, 12 -- Subject: viaWWW: spent photographic fixer
} } 8, 12 -- Content-Type: text/plain;
} charset="us-ascii"
} } ==============================End of -
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} }
} }
} }
}
}
} --
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************
}
}
}
} ==============================Original
} Headers==============================
} 8, 32 -- From mcauliff-at-umdnj.edu Fri Aug 19 11:59:45
} 2005
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} 8, 32 -- Date: Fri, 19 Aug 2005 13:00:14 -0400
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} 8, 32 -- Subject: Re: [Microscopy] viaWWW: spent
} photographic fixer
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From: winston.wiggins-at-cshs.org
Date: Fri, 19 Aug 2005 17:49:21 -0500
Subject: [Microscopy] viaWWW: spent fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (winston.wiggins-at-cshs.org) from http://www.microscopy.com/MLFormMail.html on Friday, August 19, 2005 at 12:29:21
---------------------------------------------------------------------------

Email: winston.wiggins-at-cshs.org
Name: Winston Wiggins

Organization: Cedars-Sinai Medical Center

Title-Subject: [Filtered] MListserver: spent fixer

Question: Stacey,
My understanding is that unused fixer can be sent down the drain with copious amounts of water but not spent fixer because of the silver content. I am hesitant to put anything down the drain other than water, so I've always used a silver-recovery system service.
If UC has a a Radiology Dept in its system, check with their mode of disposal or try a commercial photo lab to see what they do. They may even take it off your hands for disposal. I'd check the local regs and/or Haz Waste Dept before I put anything down the drain. It invariably comes back in your drinking water, IMO that is.
Winston Wiggins

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==============================Original Headers==============================
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From: LDUH-at-SIKORSKY.COM
Date: Fri, 19 Aug 2005 17:49:55 -0500
Subject: [Microscopy] viaWWW: Fatigue appearance in C355-T6 aluminum alloy

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (LDUH-at-SIKORSKY.COM) from http://www.microscopy.com/MLFormMail.html on Friday, August 19, 2005 at 12:43:05
---------------------------------------------------------------------------

Email: LDUH-at-SIKORSKY.COM
Name: LOU DUH

Organization: MSA / SIKORSKY AIRCRAFT

Title-Subject: [Filtered] Fatigue appearance in C355-T6 aluminum alloy.:

Question: I am currently examining an aircraft part which is made of aluminum C355-T6. I am fairly accustomed to cast aluminum, but I do not have experience wiht the fatigue appearances of this paticular alloy. What I see appears to be elongated eutectic regions with some very faint crack front markings. The surface appears somewhat rubbed and is making identification of stiation marks very difficult. Does anyone know where I can find some information about this subject - prefferably with images?? Thank you very much.

Lou

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7, 12 -- Subject: viaWWW: Fatigue appearance in C355-T6 aluminum alloy
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From: Stacey.Andringa-at-uc.edu
Date: Fri, 19 Aug 2005 17:50:27 -0500
Subject: [Microscopy] viaWWW: Thanks for all the responses -fixer

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Friday, August 19, 2005 at 14:43:45
---------------------------------------------------------------------------

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: Thanks for all the responses about spent photographic fixer. There is a researcher here who does silver recovery and will dispose of the rest as hazardous waste.

Stacey

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7, 12 -- From: Stacey.Andringa-at-uc.edu (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: Thanks for all the responses -fixer
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From: s2kdude-at-pacbell.net
Date: Fri, 19 Aug 2005 17:57:32 -0500
Subject: [Microscopy] Re: viaWWW: spent photographic fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hmmm.....There's a product called SilverMagnet SMT-20
that is compact and collects via electrolysis. And
there are also EPA certified steel wool buckets that
separate the silver out but those are mostly for low
concentration baths. Not for fixer based soln.s

Most large photo supply places will have some sort of
kit that will collect the silver that you can turn in
for a small check.

Check www.silvercouncil.org. They will direct you to
a suitable method for your use.

--- hoffpajo-at-yahoo.com wrote:

}
}
}
}
----------------------------------------------------------------------------
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} Microscopy Society of America
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}
} i remember those days when i worked in NJ, it was a
} pain in the but to collect all the spent fixer. you
} are right about kodax and the silver recovery kit.
} someone else used to do it as well but alas that
} brain
} cell is long gone.
} i do believe if you truly just want the silver in
} it's
} raw form you can use steel wool so the silver will
} react with the steel wool and bind to it. perhaps
} some
} on the list server can explain the chemistry to us
} all. i was never any good at inorganic chemisrty.
} john hoffpauir
}
}
}
} --- mcauliff-at-umdnj.edu wrote:
}
} }
} }
} }
} }
}
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} } Microscopy Society of America
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}
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} }
} } Here in New Jersey we are required to dispose of
} } used fixer as hazardous
} } waste, due to the silver content. Kodak sells (or
} } used to sell) a silver
} } recovery kit. so you might try them.
} }
} } Geoff
} }
} } Stacey.Andringa-at-uc.edu wrote:
} }
} } }
} }
}
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
} ----------------------------------------------------------------------------
} } }
} } } Below is the result of your feedback form
} } (NJZFM-ultra-55). It was submitted by
} } (Stacey.Andringa-at-uc.edu) from
} } http://www.microscopy.com/MLFormMail.html on
} } Thursday, August 18, 2005 at 09:57:24
} }
}
} ---------------------------------------------------------------------------
} } }
} } } Email: Stacey.Andringa-at-uc.edu
} } } Name: Stacey Andringa
} } }
} } } Organization: University of Cincinnati
} } }
} } } Title-Subject: [Filtered] MListserver:
} } }
} } } Question: I have been catching up on some lab
} } cleaning and have about 12 gallons of spent
} } photographic fixer. I have been told that I can
} sink
} } dispose of this using copious amounts of water or
} } pay to have our chemical disposal contractor pick
} it
} } up and dispose of it. Does anyone have a simple
} } on-site silver recovery system I could try first?
} Is
} } it worth the trouble/expense?
} } } Thanks for any help.
} } }
} } } Stacey Andringa
} } }
} } }
} }
}
} ---------------------------------------------------------------------------
} } }
} } } ==============================Original
} } Headers==============================
} } } 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 18
} } 17:28:23 2005
} } } 8, 12 -- Received: from [206.69.208.22]
} } (mac22.zaluzec.com [206.69.208.22])
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} } with ESMTP id j7IMSLGo006890
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} } } 8, 12 -- From: Stacey.Andringa-at-uc.edu (by way of
} } MicroscopyListserver)
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} fixer
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} } }
} } }
} } }
} }
} }
} } --
} } --
} } **********************************************
} } Geoff McAuliffe, Ph.D.
} } Neuroscience and Cell Biology
} } Robert Wood Johnson Medical School
} } 675 Hoes Lane, Piscataway, NJ 08854
} } voice: (732)-235-4583; fax: -4029
} } mcauliff-at-umdnj.edu
} } **********************************************
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 8, 32 -- From mcauliff-at-umdnj.edu Fri Aug 19
} 11:59:45
} } 2005
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} } 8, 32 -- Date: Fri, 19 Aug 2005 13:00:14 -0400
} } 8, 32 -- From: Geoff McAuliffe
} {mcauliff-at-umdnj.edu}
} } 8, 32 -- Subject: Re: [Microscopy] viaWWW: spent
} } photographic fixer
} } 8, 32 -- In-reply-to:
} } {200508182230.j7IMULQ8009016-at-ns.microscopy.com}
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}
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6, 15 -- From s2kdude-at-pacbell.net Fri Aug 19 17:57:31 2005
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From: smalinskas-at-yahoo.com
Date: Fri, 19 Aug 2005 20:53:15 -0500
Subject: [Microscopy] Re: Fatigue appearance in C355-T6 aluminum alloy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lou:

One good reference for fracture surfaces would be the
Metals Handbook, Ninth Edition, Volume 12,
"Fractography". Another is titled "How Components
Fail" by Donald Wulpi.

I have a lot of experience looking at fracture
surfaces both macro and micro (SEM) examination. I'd
be glad to comment on any images you may want send to
my business e-mail.

Stu Smalinskas, P.E.
Metallurgist
SKF
Plymouth, Michigan
(734) 414-6862
stu.smalinskas-at-skf.com


--- LDUH-at-SIKORSKY.COM wrote:

}
} Email: LDUH-at-SIKORSKY.COM
} Name: LOU DUH
}
} Organization: MSA / SIKORSKY AIRCRAFT
}
} Title-Subject: [Filtered] Fatigue appearance in
} C355-T6 aluminum alloy.:
}
} Question: I am currently examining an aircraft part
} which is made of aluminum C355-T6. I am fairly
} accustomed to cast aluminum, but I do not have
} experience with the fatigue appearances of this
} paticular alloy. What I see appears to be elongated
} eutectic regions with some very faint crack front
} markings. The surface appears somewhat rubbed and
} is making identification of stiation marks very
} difficult. Does anyone know where I can find some
} information about this subject - preferably with
} images?? Thank you very much.
}
} Lou
}

__________________________________________________
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8, 19 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
8, 19 -- Subject: Re: [Microscopy] Fatigue appearance in C355-T6 aluminum alloy
8, 19 -- To: LDUH-at-SIKORSKY.COM, microscopy-at-ns.microscopy.com
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==============================End of - Headers==============================




From: hoffpajo-at-yahoo.com
Date: Sat, 20 Aug 2005 09:58:15 -0500
Subject: [Microscopy] Re: viaWWW: spent photographic fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Perhaps I can put this in perspective for you. Most
states release some raw sewage. It generally happens
by accident. Either a broken pipe or a malfuntion of
the waste plant, It is not just NJ. I am not an
apologist for NJ so how the flames please,

As for the NJ reg, I started work in NJ over 15 years
ago and it was a reg back then, not sure if that is a
generation or not. You tell me. Some states, I was
working in Dallas for a lot of years allowed fixer to
be dumped down the drain when I was working there.
Perhaps some one from Texas can tell me if this is
still alowed.

I should note that mercury is released into the water
constantly, it is a by product of coal plants
producing energy. How many of us recycle batteries or
just throw them in the trash and forget about them.
there is no state that requires we recycle them.

--- John Twilley {jtwilley-at-sprynet.com} wrote:

} According to a recent article in the NYT, New Jersey
} still has annual releases of raw sewage into the
} local waterways annually in the millions of gallons.
} So perhaps it has not been long that
} they have had restrictions. In most parts of the
} country dissolved metals haven't been permitted to
} be dumped down the drain in a generation.
}
} John
}
} hoffpajo-at-yahoo.com wrote:
}
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} } To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------------
} }
} } i remember those days when i worked in NJ, it was
} a
} } pain in the but to collect all the spent fixer.
} you
} } are right about kodax and the silver recovery kit.
} } someone else used to do it as well but alas that
} brain
} } cell is long gone.
} } i do believe if you truly just want the silver in
} it's
} } raw form you can use steel wool so the silver will
} } react with the steel wool and bind to it. perhaps
} some
} } on the list server can explain the chemistry to us
} } all. i was never any good at inorganic chemisrty.
} } john hoffpauir
} }
} } --- mcauliff-at-umdnj.edu wrote:
} }
} } }
} } }
} } }
} } }
} }
}
----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
}
----------------------------------------------------------------------------
} } }
} } } Here in New Jersey we are required to dispose of
} } } used fixer as hazardous
} } } waste, due to the silver content. Kodak sells
} (or
} } } used to sell) a silver
} } } recovery kit. so you might try them.
} } }
} } } Geoff
} } }
} } } Stacey.Andringa-at-uc.edu wrote:
} } }
} } } }
} } }
} }
}
} ----------------------------------------------------------------------------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
} } } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
}
} ----------------------------------------------------------------------------
} } } }
} } } } Below is the result of your feedback form
} } } (NJZFM-ultra-55). It was submitted by
} } } (Stacey.Andringa-at-uc.edu) from
} } } http://www.microscopy.com/MLFormMail.html on
} } } Thursday, August 18, 2005 at 09:57:24
} } }
} }
}
} ---------------------------------------------------------------------------
} } } }
} } } } Email: Stacey.Andringa-at-uc.edu
} } } } Name: Stacey Andringa
} } } }
} } } } Organization: University of Cincinnati
} } } }
} } } } Title-Subject: [Filtered] MListserver:
} } } }
} } } } Question: I have been catching up on some lab
} } } cleaning and have about 12 gallons of spent
} } } photographic fixer. I have been told that I can
} sink
} } } dispose of this using copious amounts of water
} or
} } } pay to have our chemical disposal contractor
} pick it
} } } up and dispose of it. Does anyone have a simple
} } } on-site silver recovery system I could try
} first? Is
} } } it worth the trouble/expense?
} } } } Thanks for any help.
} } } }
} } } } Stacey Andringa
} } } }
} } } }
} } }
} }
}
} ---------------------------------------------------------------------------
} } } }
} } } } ==============================Original
} } } Headers==============================
} } } } 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 18
} } } 17:28:23 2005
} } } } 8, 12 -- Received: from [206.69.208.22]
} } } (mac22.zaluzec.com [206.69.208.22])
} } } } 8, 12 -- by ns.microscopy.com
} (8.12.11/8.12.8)
} } } with ESMTP id j7IMSLGo006890
} } } } 8, 12 -- for {microscopy-at-microscopy.com} ;
} Thu, 18
} } } Aug 2005 17:28:22 -0500
} } } } 8, 12 -- Mime-Version: 1.0
} } } } 8, 12 -- X-Sender: (Unverified)
} } } } 8, 12 -- Message-Id:
} } } {p06110400bf2abbf3cfd8-at-[206.69.208.22]}
} } } } 8, 12 -- Date: Thu, 18 Aug 2005 17:28:21 -0500
} } } } 8, 12 -- To: microscopy-at-microscopy.com
} } } } 8, 12 -- From: Stacey.Andringa-at-uc.edu (by way
} of
} } } MicroscopyListserver)
} } } } 8, 12 -- Subject: viaWWW: spent photographic
} fixer
} } } } 8, 12 -- Content-Type: text/plain;
} } } charset="us-ascii"
} } } } ==============================End of -
} } } Headers==============================
} } } }
} } } }
} } } }
} } }
} } }
} } } --
} } } --
} } } **********************************************
} } } Geoff McAuliffe, Ph.D.
} } } Neuroscience and Cell Biology
} } } Robert Wood Johnson Medical School
} } } 675 Hoes Lane, Piscataway, NJ 08854
} } } voice: (732)-235-4583; fax: -4029
} } } mcauliff-at-umdnj.edu
} } } **********************************************
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
} } } 8, 32 -- From mcauliff-at-umdnj.edu Fri Aug 19
} 11:59:45
} } } 2005
} } } 8, 32 -- Received: from mail01.umdnj.edu
} } } (zix01.UMDNJ.EDU [130.219.34.124])
} } } 8, 32 -- by ns.microscopy.com
} (8.12.11/8.12.8) with
} } } ESMTP id j7JGxjmx017595
} } } 8, 32 -- for
} {microscopy-at-msa.microscopy.com} ; Fri,
} } } 19 Aug 2005 11:59:45 -0500
} } } 8, 32 -- Received: from zix01.umdnj.edu (ZixVPM
} } } [127.0.0.1])
} } } 8, 32 -- by Outbound.umdnj.edu
} (Proprietary) with
} } } ESMTP id B17EEEC05B
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} } } 19 Aug 2005 12:59:44 -0400 (EDT)
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} } } 19 Aug 2005 12:59:42 -0400 (EDT)
} } } 8, 32 -- Received: from
} } } conversion-daemon.Polaris.umdnj.edu by
} } } Polaris.umdnj.edu
}
=== message truncated ===




____________________________________________________
Start your day with Yahoo! - make it your home page
http://www.yahoo.com/r/hs


==============================Original Headers==============================
8, 19 -- From hoffpajo-at-yahoo.com Sat Aug 20 09:58:14 2005
8, 19 -- Received: from web50201.mail.yahoo.com (web50201.mail.yahoo.com [206.190.38.42])
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8, 19 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
8, 19 -- Subject: Re: [Microscopy] viaWWW: spent photographic fixer
8, 19 -- To: John Twilley {jtwilley-at-sprynet.com} , microscopy-at-msa.microscopy.com
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From: jtwilley-at-sprynet.com
Date: Sat, 20 Aug 2005 11:39:20 -0500
Subject: [Microscopy] Re: viaWWW: spent photographic fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

So your point is? "Just do it if you can get away with it" and can point to
something else that's worse?

I pointed out, off list, to the original inquirer that most jurisdictions limit
silver (in spent fixer) because it is a biocide that can cause a die-off of the
bacteria that are required for the normal functioning of waste water treatment
plants.

As an alternative to paying a waste hauler to remove fixer, one can sign up a
precious metal recovery company to remove it. Usually this is a break-even
proposition with the recovery firm providing a container and pickup service in
exchange for keeping the silver and handling the disposal of the silver-free
remains.

John

john hoffpauir wrote:

} Perhaps I can put this in perspective for you. Most
} states release some raw sewage. It generally happens
} by accident. Either a broken pipe or a malfuntion of
} the waste plant, It is not just NJ. I am not an
} apologist for NJ so how the flames please,
}
} As for the NJ reg, I started work in NJ over 15 years
} ago and it was a reg back then, not sure if that is a
} generation or not. You tell me. Some states, I was
} working in Dallas for a lot of years allowed fixer to
} be dumped down the drain when I was working there.
} Perhaps some one from Texas can tell me if this is
} still alowed.
}
} I should note that mercury is released into the water
} constantly, it is a by product of coal plants
} producing energy. How many of us recycle batteries or
} just throw them in the trash and forget about them.
} there is no state that requires we recycle them.
}
} --- John Twilley {jtwilley-at-sprynet.com} wrote:
}
} } According to a recent article in the NYT, New Jersey
} } still has annual releases of raw sewage into the
} } local waterways annually in the millions of gallons.
} } So perhaps it has not been long that
} } they have had restrictions. In most parts of the
} } country dissolved metals haven't been permitted to
} } be dumped down the drain in a generation.
} }
} } John
} }
} } hoffpajo-at-yahoo.com wrote:
} }
} } }
} }
} ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
} ----------------------------------------------------------------------------
} } }
} } } i remember those days when i worked in NJ, it was
} } a
} } } pain in the but to collect all the spent fixer.
} } you
} } } are right about kodax and the silver recovery kit.
} } } someone else used to do it as well but alas that
} } brain
} } } cell is long gone.
} } } i do believe if you truly just want the silver in
} } it's
} } } raw form you can use steel wool so the silver will
} } } react with the steel wool and bind to it. perhaps
} } some
} } } on the list server can explain the chemistry to us
} } } all. i was never any good at inorganic chemisrty.
} } } john hoffpauir
} } }
} } } --- mcauliff-at-umdnj.edu wrote:
} } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
} ----------------------------------------------------------------------------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } } Microscopy Society of America
} } } } To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} } } }
} } }
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } }
} }
} ----------------------------------------------------------------------------
} } } }
} } } } Here in New Jersey we are required to dispose of
} } } } used fixer as hazardous
} } } } waste, due to the silver content. Kodak sells
} } (or
} } } } used to sell) a silver
} } } } recovery kit. so you might try them.
} } } }
} } } } Geoff
} } } }
} } } } Stacey.Andringa-at-uc.edu wrote:
} } } }
} } } } }
} } } }
} } }
} }
} } ----------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- CoSponsor: The
} } } } Microscopy Society of America
} } } } } To Subscribe/Unsubscribe --
} } } } http://www.microscopy.com/MicroscopyListserver
} } } } } On-Line Help
} } } }
} } }
} }
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } }
} }
} } ----------------------------------------------------------------------------
} } } } }
} } } } } Below is the result of your feedback form
} } } } (NJZFM-ultra-55). It was submitted by
} } } } (Stacey.Andringa-at-uc.edu) from
} } } } http://www.microscopy.com/MLFormMail.html on
} } } } Thursday, August 18, 2005 at 09:57:24
} } } }
} } }
} }
} } ---------------------------------------------------------------------------
} } } } }
} } } } } Email: Stacey.Andringa-at-uc.edu
} } } } } Name: Stacey Andringa
} } } } }
} } } } } Organization: University of Cincinnati
} } } } }
} } } } } Title-Subject: [Filtered] MListserver:
} } } } }
} } } } } Question: I have been catching up on some lab
} } } } cleaning and have about 12 gallons of spent
} } } } photographic fixer. I have been told that I can
} } sink
} } } } dispose of this using copious amounts of water
} } or
} } } } pay to have our chemical disposal contractor
} } pick it
} } } } up and dispose of it. Does anyone have a simple
} } } } on-site silver recovery system I could try
} } first? Is
} } } } it worth the trouble/expense?
} } } } } Thanks for any help.
} } } } }
} } } } } Stacey Andringa
} } } } }
} } } } }
} } } }
} } }
} }
} } ---------------------------------------------------------------------------
} } } } }
} } } } } ==============================Original
} } } } Headers==============================
} } } } } 8, 12 -- From zaluzec-at-microscopy.com Thu Aug 18
} } } } 17:28:23 2005
} } } } } 8, 12 -- Received: from [206.69.208.22]
} } } } (mac22.zaluzec.com [206.69.208.22])
} } } } } 8, 12 -- by ns.microscopy.com
} } (8.12.11/8.12.8)
} } } } with ESMTP id j7IMSLGo006890
} } } } } 8, 12 -- for {microscopy-at-microscopy.com} ;
} } Thu, 18
} } } } Aug 2005 17:28:22 -0500
} } } } } 8, 12 -- Mime-Version: 1.0
} } } } } 8, 12 -- X-Sender: (Unverified)
} } } } } 8, 12 -- Message-Id:
} } } } {p06110400bf2abbf3cfd8-at-[206.69.208.22]}
} } } } } 8, 12 -- Date: Thu, 18 Aug 2005 17:28:21 -0500
} } } } } 8, 12 -- To: microscopy-at-microscopy.com
} } } } } 8, 12 -- From: Stacey.Andringa-at-uc.edu (by way
} } of
} } } } MicroscopyListserver)
} } } } } 8, 12 -- Subject: viaWWW: spent photographic
} } fixer
} } } } } 8, 12 -- Content-Type: text/plain;
} } } } charset="us-ascii"
} } } } } ==============================End of -
} } } } Headers==============================
} } } } }
} } } } }
} } } } }
} } } }
} } } }
} } } } --
} } } } --
} } } } **********************************************
} } } } Geoff McAuliffe, Ph.D.
} } } } Neuroscience and Cell Biology
} } } } Robert Wood Johnson Medical School
} } } } 675 Hoes Lane, Piscataway, NJ 08854
} } } } voice: (732)-235-4583; fax: -4029
} } } } mcauliff-at-umdnj.edu
} } } } **********************************************
} } } }
} } } }
} } } }
} } } } ==============================Original
} } } } Headers==============================
} } } } 8, 32 -- From mcauliff-at-umdnj.edu Fri Aug 19
} } 11:59:45
} } } } 2005
} } } } 8, 32 -- Received: from mail01.umdnj.edu
} } } } (zix01.UMDNJ.EDU [130.219.34.124])
} } } } 8, 32 -- by ns.microscopy.com
} } (8.12.11/8.12.8) with
} } } } ESMTP id j7JGxjmx017595
} } } } 8, 32 -- for
} } {microscopy-at-msa.microscopy.com} ; Fri,
} } } } 19 Aug 2005 11:59:45 -0500
} } } } 8, 32 -- Received: from zix01.umdnj.edu (ZixVPM
} } } } [127.0.0.1])
} } } } 8, 32 -- by Outbound.umdnj.edu
} } (Proprietary) with
} } } } ESMTP id B17EEEC05B
} } } } 8, 32 -- for
} } {microscopy-at-msa.microscopy.com} ; Fri,
} } } } 19 Aug 2005 12:59:44 -0400 (EDT)
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} } } } (polarisa.UMDNJ.EDU [130.219.34.131])
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} } } } 19 Aug 2005 12:59:42 -0400 (EDT)
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} } } } conversion-daemon.Polaris.umdnj.edu by
} } } } Polaris.umdnj.edu
} }
} === message truncated ===
}
}
} ____________________________________________________
} Start your day with Yahoo! - make it your home page
} http://www.yahoo.com/r/hs




==============================Original Headers==============================
9, 19 -- From jtwilley-at-sprynet.com Sat Aug 20 11:39:20 2005
9, 19 -- Received: from pop-canoe.atl.sa.earthlink.net (pop-canoe.atl.sa.earthlink.net [207.69.195.66])
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From: hoffpajo-at-yahoo.com
Date: Sat, 20 Aug 2005 11:57:20 -0500
Subject: [Microscopy] Re: viaWWW: spent photographic fixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am not certain what your point is. All i was doing
was pointing out to you that NJ has regs on how to
deal with spent fixer. I was not suggesting that
anyone cheat on proper wast disposal. Your suggestion
that I was is offensive.
FYI I know why silver is prohibited. Don't put words
in my emails that are not there. I already have enough
trouble typing.

As far as I am concerned labs should go digital. Alot
of major hospitals already have.
John Hoffpauit

--- John Twilley {jtwilley-at-sprynet.com} wrote:

} So your point is? "Just do it if you can get away
} with it" and can point to
} something else that's worse?
}
} I pointed out, off list, to the original inquirer
} that most jurisdictions limit
} silver (in spent fixer) because it is a biocide that
} can cause a die-off of the
} bacteria that are required for the normal
} functioning of waste water treatment
} plants.
}
} As an alternative to paying a waste hauler to remove
} fixer, one can sign up a
} precious metal recovery company to remove it.
} Usually this is a break-even
} proposition with the recovery firm providing a
} container and pickup service in
} exchange for keeping the silver and handling the
} disposal of the silver-free
} remains.
}
} John
}
} john hoffpauir wrote:
}
} } Perhaps I can put this in perspective for you.
} Most
} } states release some raw sewage. It generally
} happens
} } by accident. Either a broken pipe or a malfuntion
} of
} } the waste plant, It is not just NJ. I am not an
} } apologist for NJ so how the flames please,
} }
} } As for the NJ reg, I started work in NJ over 15
} years
} } ago and it was a reg back then, not sure if that
} is a
} } generation or not. You tell me. Some states, I was
} } working in Dallas for a lot of years allowed fixer
} to
} } be dumped down the drain when I was working there.
} } Perhaps some one from Texas can tell me if this is
} } still alowed.
} }
} } I should note that mercury is released into the
} water
} } constantly, it is a by product of coal plants
} } producing energy. How many of us recycle batteries
} or
} } just throw them in the trash and forget about
} them.
} } there is no state that requires we recycle them.
} }
} } --- John Twilley {jtwilley-at-sprynet.com} wrote:
} }
} } } According to a recent article in the NYT, New
} Jersey
} } } still has annual releases of raw sewage into the
} } } local waterways annually in the millions of
} gallons.
} } } So perhaps it has not been long that
} } } they have had restrictions. In most parts of
} the
} } } country dissolved metals haven't been permitted
} to
} } } be dumped down the drain in a generation.
} } }
} } } John
} } }
} } } hoffpajo-at-yahoo.com wrote:
} } }
} } } }
} } }
} }
}
----------------------------------------------------------------------------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of America
} } } } To Subscribe/Unsubscribe --
} } } http://www.microscopy.com/MicroscopyListserver
} } } } On-Line Help
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } }
} }
}
----------------------------------------------------------------------------
} } } }
} } } } i remember those days when i worked in NJ, it
} was
} } } a
} } } } pain in the but to collect all the spent
} fixer.
} } } you
} } } } are right about kodax and the silver recovery
} kit.
} } } } someone else used to do it as well but alas
} that
} } } brain
} } } } cell is long gone.
} } } } i do believe if you truly just want the silver
} in
} } } it's
} } } } raw form you can use steel wool so the silver
} will
} } } } react with the steel wool and bind to it.
} perhaps
} } } some
} } } } on the list server can explain the chemistry
} to us
} } } } all. i was never any good at inorganic
} chemisrty.
} } } } john hoffpauir
} } } }
} } } } --- mcauliff-at-umdnj.edu wrote:
} } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } }
} } }
} }
}
----------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- CoSponsor: The
} } } } } Microscopy Society of America
} } } } } To Subscribe/Unsubscribe --
} } } } }
} http://www.microscopy.com/MicroscopyListserver
} } } } } On-Line Help
} } } } }
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } } }
} } } }
} } }
} }
}
----------------------------------------------------------------------------
} } } } }
} } } } } Here in New Jersey we are required to
} dispose of
} } } } } used fixer as hazardous
} } } } } waste, due to the silver content. Kodak
} sells
} } } (or
} } } } } used to sell) a silver
} } } } } recovery kit. so you might try them.
} } } } }
} } } } } Geoff
} } } } }
} } } } } Stacey.Andringa-at-uc.edu wrote:
} } } } }
} } } } } }
} } } } }
} } } }
} } }
} }
}
} ----------------------------------------------------------------------------
} } } } } } The Microscopy ListServer -- CoSponsor:
} The
} } } } } Microscopy Society of America
} } } } } } To Subscribe/Unsubscribe --
} } } } }
} http://www.microscopy.com/MicroscopyListserver
} } } } } } On-Line Help
} } } } }
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } } }
} } } }
} } }
} }
}
} ----------------------------------------------------------------------------
} } } } } }
} } } } } } Below is the result of your feedback form
} } } } } (NJZFM-ultra-55). It was submitted by
} } } } } (Stacey.Andringa-at-uc.edu) from
} } } } } http://www.microscopy.com/MLFormMail.html on
} } } } } Thursday, August 18, 2005 at 09:57:24
} } } } }
} } } }
} } }
} }
}
} ---------------------------------------------------------------------------
} } } } } }
} } } } } } Email: Stacey.Andringa-at-uc.edu
} } } } } } Name: Stacey Andringa
} } } } } }
} } } } } } Organization: University of Cincinnati
} } } } } }
} } } } } } Title-Subject: [Filtered] MListserver:
} } } } } }
} } } } } } Question: I have been catching up on some
} lab
} } } } } cleaning and have about 12 gallons of spent
}
=== message truncated ===




____________________________________________________
Start your day with Yahoo! - make it your home page
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==============================Original Headers==============================
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7, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
7, 20 -- Subject: Re: [Microscopy] viaWWW: spent photographic fixer
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From: pollingmel-at-aol.com
Date: Mon, 22 Aug 2005 07:58:20 -0500
Subject: [Microscopy] viaWWW: What are Geffner Pintubes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

You may find that you are able to punch through the surface deformation by
increasing the kV. The backscatter contribution to the image will increase
and if you are lucky, and the surface damage does not go too
deep, you may see striations more clearly.

Good Luck

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com

----- Original Message -----
X-from: {LDUH-at-SIKORSKY.COM}
To: {protrain-at-emcourses.com}
Sent: Friday, August 19, 2005 11:51 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pollingmel-at-aol.com) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Sunday, August 21, 2005 at 16:30:13
---------------------------------------------------------------------------

Email: pollingmel-at-aol.com
Name: Mel Pollinger

Organization: New York Microscopical Society

Title-Subject: [Filtered] MListserver:

Question: Anyone know anything about a product called Geffner Pintubes? They are glass tubes, 7mm O.D. X 10cm L. flame sealed at both ends. Got a bunch of these recently, but have no idea what they are or were for.

Thanks for any clue,
Mel Pollinger

---------------------------------------------------------------------------

==============================Original Headers==============================
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==============================End of - Headers==============================




From: K.venner-at-ion.ucl.ac.uk
Date: Mon, 22 Aug 2005 07:59:35 -0500
Subject: [Microscopy] viaWWW: TEM tissue processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://www.microscopy.com/MLFormMail.html on Monday, August 22, 2005 at 04:07:43
---------------------------------------------------------------------------

Email: K.venner-at-ion.ucl.ac.uk
Name: k.venner

Organization: ucl London uk

Title-Subject: [Filtered] MListserver: TEM tissue processors

Question: We have had a Lynx for nearly 20 years, and during that time I have used it at least once a week, sometimes 2 or 3 times per week. It has needed a replacement mother board once, and we have replaced the vial seal a couple of times. We only go up to 1:1 resin/propylene oxide as we found the neat resin step messy, so we unload at the end of the 1:1 stage into clean processing vials with neat resin. We found it too risky to do unattended runs overnight, as once a vial was misaligned and the samples dried out as the carousel was unable to move to the next vial, but that was operator error rather than a fault with the equipment. We also found that the propylene oxide evaporated too quickly for an overnight run. We do re-use the vials and I clean the baskets and vials with acetone, which works really well, only discarding them when they become too black or loose in the carousel.

I really like the Lynx, and we give space to a newer second one, which is also really handy for the future....

I have seen the Leica version, but have no experience using the machine. They are pretty much similar in design, and the consumables are virtually interchangeable.

It is economic with chemicals and in our hands, reliable. The only limit is the size and dimensions of your samples you want to process.

---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Mon Aug 22 07:59:35 2005
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9, 12 -- Subject: viaWWW: TEM tissue processors
9, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: protrain-at-emcourses.com
Date: Mon, 22 Aug 2005 13:44:25 -0500
Subject: [Microscopy] FEI SEM Venue Wanted in UK

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I know many of the UK microscopists read the listserver so here is my plea!

I am looking for a material science laboratory in the UK that uses an FEI
SEM and EDS. I have two material science overseas customers who wish to
take an advanced SEM + EDS training course in the UK and unfortunately all
of my clients with these instruments are biologists!

In exchange for letting us use the instrument we are willing to offer two
places on the course for the laboratory to use. We would need the
microscope each afternoon for 5 days and a small seminar room or office each
morning for the lectures.

If the establishment wishes we are able to carry out the lecture side of the
course for as many people as they may wish to attend, only the practicals
periods would be limited in numbers.

Thanks listserver sorry to take up space.

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7802 966067 Web www.emcourses.com


==============================Original Headers==============================
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8, 22 -- From: "Steve Chapman" {protrain-at-emcourses.com}
8, 22 -- To: "American Soc" {microscopy-at-microscopy.com}
8, 22 -- Subject: FEI SEM Venue Wanted in UK
8, 22 -- Date: Mon, 22 Aug 2005 19:43:15 +0100
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From: Margaret.HargerAllen-at-med.va.gov
Date: Mon, 22 Aug 2005 13:56:07 -0500
Subject: [Microscopy] TEM of aspiration biopsies - remove blood

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our staff cytopathologist was wondering if there was an EM fixative
available that was similar to "Cytorich Red" fixative for light microscopy.
This formalin based fixative lyses the red blood cells from the fine needle
aspiration biopsies and leaves the tumor cells for the cell blocks and
histology work. He wanted to try this for his EM specimens, but
unfortunately this contains formaldehyde, not gluteraldehyde.

Is there something similar out there for EM?

Thanks, Peggy Harger-Allen

EM Lab

==============================Original Headers==============================
4, 17 -- From Margaret.HargerAllen-at-med.va.gov Mon Aug 22 13:56:06 2005
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4, 17 -- From: "Harger-Allen, Margaret" {Margaret.HargerAllen-at-med.va.gov}
4, 17 -- To: microscopy-at-microscopy.com
4, 17 -- Subject: TEM of aspiration biopsies - remove blood
4, 17 -- Date: Mon, 22 Aug 2005 13:59:33 -0500
4, 17 -- MIME-Version: 1.0
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From: pbarber-at-bu.edu
Date: Mon, 22 Aug 2005 17:54:00 -0500
Subject: [Microscopy] viaWWW: digital SLR to a c-mount on a stereo microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pbarber-at-bu.edu) from http://www.microscopy.com/MLFormMail.html on Monday, August 22, 2005 at 11:05:10
---------------------------------------------------------------------------

Email: pbarber-at-bu.edu
Name: Paul Barber

Organization: Boston University

Title-Subject: [Filtered] MListserver:

Question: I would like to be able to attach a digital SLR (ie Nikon D100) to a c-mount on a stereo microscope like a Leica MZ9.5 or Nikon SMZ800. I've heard conflicting answers from product reps, some saying that it isn't possible, some saying it is? However, I have no experience at all microscopy, but need to buy a system that has at least some photodocumentation ability. I don't need (and can't afford) a 4-5000 dollar imaging system. It seems like putting a digital SLR on a c-mount would be a reasonable and less expensive way to do photodocumentation.

Thanks
Paul

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Mon Aug 22 17:54:00 2005
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7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: ramesh-nair-at-uiowa.edu
Date: Mon, 22 Aug 2005 17:54:37 -0500
Subject: [Microscopy] viaWWW: EM technologist position open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ramesh-nair-at-uiowa.edu) from http://www.microscopy.com/MLFormMail.html on Monday, August 22, 2005 at 14:41:19
---------------------------------------------------------------------------

Email: ramesh-nair-at-uiowa.edu
Name: Ramesh Nair

Organization: University of Iowa

Title-Subject: [Filtered] EM technologist position oprn

Question: Our EM tech is retiring in November. Therefore a position for EM tech is available. If anybody is interested please contact me:

Ramesh Nair, M.D.
Director, Renal Pathology/EM service
University of Iowa

319 330 3102
ramesh-nair-at-uiowa.edu


---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Mon Aug 22 17:54:36 2005
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9, 12 -- From: ramesh-nair-at-uiowa.edu (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: EM technologist position open
9, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: Henrik.Kaker-at-guest.arnes.si
Date: Tue, 23 Aug 2005 07:28:40 -0500
Subject: [Microscopy] viaWWW: Jeol JSM 35-CF scan generator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Henrik.Kaker-at-guest.arnes.si) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 23, 2005 at 04:17:40
---------------------------------------------------------------------------

Email: Henrik.Kaker-at-guest.arnes.si
Name: Henrik Kaker

Organization: Metal Ravne

Title-Subject: [Filtered] Jeol JSM 35-CF scan generator

Question: Hello All,

We are looking the information about the name and pin configurations of connector(External Beam Interface) on the Jeol JSM 35-CF scan generator. We wish to connect external scan generator.

Thank you,

Henrik Kaker
SEM-EDS Lab
Metal Ravne
Slovenia


---------------------------------------------------------------------------

==============================Original Headers==============================
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10, 12 -- From: Henrik.Kaker-at-guest.arnes.si (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: Jeol JSM 35-CF scan generator
10, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 23 Aug 2005 10:59:08 -0500
Subject: [Microscopy] Trolling for SEM/STEM specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sending again.... I figure many folks were in HI.

----------------------------------------------

I am again looking for prepared and interesting
specimens for SEM/STEM or just TEM negs. Human pathogens
are especially welcome. Standard 12mm pin stubs for
SEM are perfect. Bacteria, parasites, cancers, etc.
are good. CPD/fixed specimens coated or un-coated
are fine.

Pls contact me off-line for my payment schedule
and what you may have to sell (I keep it) or rent
(in case you want it back).

gary g.


==============================Original Headers==============================
6, 17 -- From gary-at-gaugler.com Tue Aug 23 10:59:08 2005
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6, 17 -- Subject: Trolling for SEM/STEM specimens
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From: brian.keller-at-sw.ca
Date: Tue, 23 Aug 2005 17:55:56 -0500
Subject: [Microscopy] viaWWW: microdensitometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (brian.keller-at-sw.ca) from http://www.microscopy.com/MLFormMail.html on Tuesday, August 23, 2005 at 14:27:50
---------------------------------------------------------------------------

Email: brian.keller-at-sw.ca
Name: Brian

Organization: Hospital

Title-Subject: [Filtered] MListserver: microdensitometer

Question: I am interested in purchasing a high resolution microdensitometer for analyzing x-ray films. Can anyone suggest some company's for me. Thanks for any help towards this.

Regards,
Brian

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 12 -- From zaluzec-at-microscopy.com Tue Aug 23 17:55:56 2005
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7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: brian.keller-at-sw.ca (by way of MicroscopyListserver)
7, 12 -- Subject: viaWWW: microdensitometer
7, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Tue, 23 Aug 2005 19:32:28 -0500
Subject: [Microscopy] Re: viaWWW: microdensitometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I use Macbeth densitometers and like them very much.
While I have an old one, there are newer versions
available. Check out:

http://usa.gretagmacbethstore.com/

I think that these have been a big standard for
a long time.

gary g.


At 03:59 PM 8/23/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
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10, 19 -- Subject: Re: [Microscopy] viaWWW: microdensitometer
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From: kunli218-at-yahoo.com.sg
Date: Wed, 24 Aug 2005 08:48:11 -0500
Subject: [Microscopy] viaWWW: TiAL alloy/intermetallic electrical resistance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kunli218-at-yahoo.com.sg) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, August 24, 2005 at 08:42:53
---------------------------------------------------------------------------

Email: kunli218-at-yahoo.com.sg
Name: Simon Lee

Title-Subject: [Filtered] MListserver: TiAL alloy/intermetallic electrical resistance

Question: Dear Listers,

I'd like to know the electricla resitance of TiAL alloy with different Al content? Any reference to recommend? Is it necessary for TiAL alloy or intermetallic to form at very high temperature (} 800 C) or is it possible for TiAl alloy to form at relatively low temperature (say 400 C)?

Your help is appreciated!1

Simon

Chartered Semicon

---------------------------------------------------------------------------

==============================Original Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Wed Aug 24 08:48:10 2005
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9, 12 -- From: kunli218-at-yahoo.com.sg (by way of MicroscopyListserver)
9, 12 -- Subject: viaWWW: TiAL alloy/intermetallic electrical resistance
9, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: hoffpajo-at-yahoo.com
Date: Wed, 24 Aug 2005 12:43:53 -0500
Subject: [Microscopy] Re: Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

} Dear John,
}
} Nestor has established a special filter to block all
} my messages not only
} to the ListServer but also emails addressed
} personally to him. He also
} denied me an explanation of what I did wrong.
} Therefore, I would like to
} ask the EM community to help me reinstate my name
} and membership at
} ListServer. If you feel comfortable, I would like
} to ask you to publish my
} letter on ListServer. I don't want to cause you any
} problems, so I suggest
} that if you decide to publish my letter, ask
} Nestor's permission first. I
} would also appreciate your opinion on this letter
} and would be happy to
} make corrections, clarifications, etc. Please feel
} free to tell me if this
} idea doesn't seem suitable and you would prefer not
} to participate. It's
} perfectly fine; I'll find other way to make my
} letter available to EM
} community. I would like to make it clear to my
} fellow microscopists that
} they may be treated in a similar way on ListServer
} if something is not
} done. For this reason, I want to speak directly to
} the people on the
} ListServer, not just Nestor. I also sincerely want
} clarification on what I
} did wrong and why I was boycotted in such harsh way.
} Thanks for your
} understanding and help. Sergey.
}
}
==============================================================
}
} Open letter to EM community:
}
}
} August
} 23, 2005
}
} Dear colleagues,
}
} It will soon be one month since I was disconnected
} from the EM
} community. I am asking for you to support
} reinstatement of my name and
} membership.
}
} As many of you are aware, Nestor cancelled my
} subscription to the
} Microscopy ListServer without a sufficiently clear
} explanation as to what I
} did wrong to the degree that cancellation of my
} membership was necessary. I
} didn't start the thread in question or participate
} in the thread, but
} commented only on the direction of the thread.
}
} After my membership was cancelled, I sent a private
} letter to Nestor asking
} him to explain in detail what was wrong in my recent
} postings, so I could
} adjust my behavior accordingly. He declined to
} answer my questions and
} immediately after installed a filter to block my
} messages. Since I lost
} the ability to communicate with Nestor directly, I
} am asking for your
} support in helping me to reinstate my membership at
} the EM ListServer.
}
} Many of you sent me copies of your emails to the
} ListServer asking Nestor
} to reinstate my membership. Many thanks for that
} support. I sincerely
} believe that a public place, especially a scientific
} forum such as the
} Microscopy ListServer, should not be ruled by any
} single person. Decisions
} should be made based on agreed upon principles that
} are established by the
} EM community. Please help me to reinstate my name
} and membership at
} ListServer.
}
} Thanks for your support. Sergey
}
}
} Here is the correspondence between Nestor and me
} that was on the ListServer
} and which presumably led to my membership
} cancellation. The most recent
} emails are on top. For simplicity's sake, I deleted
} duplicate email repeats
} and most headers. The original messages may be
} obtained from me or through
} ListServer:
}
} Tue Jul 26 11:51:32 2005
} I apologize to the list for posting Marilyn vos
} Savant's statement on
} microscopy. I just wanted to share the article
} because I thought it was
} humorous...I shall refrain from such postings.
} best,
} Beth
}
}
------------------------------------------------------------------------------------------------------------------
} 7/26/2005
} Beth & everyone else
}
} There was no problem with Beth's initial posting
} and no apology was
} needed here Beth. Sorry if I appeared to have come
} down on you.
} The followups IMHO were starting to drift into
} critiques of Marilyn whomever
} and her "crap Journalism" and I was trying to nip
} that direction of
} critique in the bud. However, I see I had the
} opposite effect. Oh well.....
} As Dorrance said, everyone can use a grin
} occassionally .
}
} Nestor
}
}
---------------------------------------------------------------------------------------------------
}
} Nestor
} I don't see any reason why we could not discuss the
} quality of somebody's
} work even if it's a journalist with former (?) high
} IQ. Is it prohibited
} to discuss people's work with high IQ on this
} ListServer? Sergey
}
--------------------------------------------------------------------------------------------
}
} 05:10 PM 7/26/2005
} Sergey
} A honest discussion or even disagreement about a
} subject or work which is
} microscopy
} related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally
} disparage any person or
} organization on the Listserver. This rule applies
} equally to Journalists
} as well as Microscopists. Please review the rules
} which you received
} upon subscription.
}
{http://microscopy.com/MicroscopyListserver/Rules.html} http://microscopy.com/MicroscopyListserver/Rules.html
} Presumably a person of hi IQ has the ability to
} distinguish between these two.
} Nestor
} Your Friendly Neighborhood SysOp
}
}
---------------------------------------------------------------------------------------------------------------------
}
} 7/26/05
} My IQ is very low, Nestor, so I am electron
} microscopist for last 30
} years... Is against rules to say truth? You know,
} truth sometime hurts
} and may be politically incorrect... This forum
} becomes too much politically
} correct and heavily censured (yes, it's true), so I
} am loosing interest
} here... I had enough censorship and "rules" in
} USSR. But, I have to admit
} US is over performing USSR now, so I feel I am at
} home. Sergey
}
-------------------------------------------------------------------------------------------------
}
} Date: Tue, 26 Jul 2005 21:02:29 -0500
} To: sryazant-at-ucla.edu
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} Subject: [Microscopy] Re: Discussion of high IQ vs
} Disparagement of a
}
} Sergy
}
} I don't care who you are or where your from, but I
} insist the rules which
} have been established for over a decade are
} followed. Since you believe
} you are above the rules so be it. You are welcome
} togo elsewhere.
}
} I have canceled your subscription to the Listserver.
}
}
} Nestor
}
}
---------------------------------------------------------------------------------------------------------------------
}
} 7:36 PM 7/26/05
}
=== message truncated ===


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
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6, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 20 -- Subject: Re: [Microscopy] Discussion of high IQ vs Disparagement of a
6, 20 -- To: microscopy-at-microscopy.com
6, 20 -- Cc: zaluzec-at-microscopy.com
6, 20 -- In-Reply-To: {6.1.2.0.2.20050823233808.055a16f0-at-mail.ucla.edu}
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From: Jane.LaGoy-at-bodycote.com
Date: Wed, 24 Aug 2005 13:54:22 -0500
Subject: [Microscopy] freedom of speech?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

IMHO it's a sad state of affairs that I am afraid to make any bold statement
for fear that I too will be censured, thrown off the Microscopy List and
denied any forum for appeal. Kind of mimics the current climate in our
national government....If this turns out to be my last communication, I want
to thank you all for your open and willing sharing of so much microscopy
knowledge. --Jane LaGoy

--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

} Dear John,
}
} Nestor has established a special filter to block all
} my messages not only
} to the ListServer but also emails addressed
} personally to him. He also
} denied me an explanation of what I did wrong.
} Therefore, I would like to
} ask the EM community to help me reinstate my name
} and membership at
} ListServer. If you feel comfortable, I would like
} to ask you to publish my
} letter on ListServer. I don't want to cause you any
} problems, so I suggest
} that if you decide to publish my letter, ask
} Nestor's permission first. I
} would also appreciate your opinion on this letter
} and would be happy to
} make corrections, clarifications, etc. Please feel
} free to tell me if this
} idea doesn't seem suitable and you would prefer not
} to participate. It's
} perfectly fine; I'll find other way to make my
} letter available to EM
} community. I would like to make it clear to my
} fellow microscopists that
} they may be treated in a similar way on ListServer
} if something is not
} done. For this reason, I want to speak directly to
} the people on the
} ListServer, not just Nestor. I also sincerely want
} clarification on what I
} did wrong and why I was boycotted in such harsh way.
} Thanks for your
} understanding and help. Sergey.
}
==============================================================
}
} Open letter to EM community:
}
}
} August 23, 2005
}
} Dear colleagues,
}
} It will soon be one month since I was disconnected
} from the EM
} community. I am asking for you to support
} reinstatement of my name and membership.
}
} As many of you are aware, Nestor cancelled my
} subscription to the
} Microscopy ListServer without a sufficiently clear
} explanation as to what I
} did wrong to the degree that cancellation of my
} membership was necessary. I
} didn't start the thread in question or participate
} in the thread, but
} commented only on the direction of the thread.
}
} After my membership was cancelled, I sent a private
} letter to Nestor asking
} him to explain in detail what was wrong in my recent
} postings, so I could
} adjust my behavior accordingly. He declined to
} answer my questions and
} immediately after installed a filter to block my
} messages. Since I lost
} the ability to communicate with Nestor directly, I
} am asking for your
} support in helping me to reinstate my membership at
} the EM ListServer.
}
} Many of you sent me copies of your emails to the
} ListServer asking Nestor
} to reinstate my membership. Many thanks for that
} support. I sincerely
} believe that a public place, especially a scientific
} forum such as the
} Microscopy ListServer, should not be ruled by any
} single person. Decisions
} should be made based on agreed upon principles that
} are established by the
} EM community. Please help me to reinstate my name
} and membership at ListServer.
}
} Thanks for your support. Sergey
}
}
} Here is the correspondence between Nestor and me
} that was on the ListServer
} and which presumably led to my membership
} cancellation. The most recent
} emails are on top. For simplicity's sake, I deleted
} duplicate email repeats
} and most headers. The original messages may be
} obtained from me or through ListServer:
}
} Tue Jul 26 11:51:32 2005
} I apologize to the list for posting Marilyn vos
} Savant's statement on
} microscopy. I just wanted to share the article
} because I thought it was
} humorous...I shall refrain from such postings.
} best, Beth
}
----------------------------------------------------------------------------
---------------------
} 7/26/2005
} Beth & everyone else
}
} There was no problem with Beth's initial posting
} and no apology was
} needed here Beth. Sorry if I appeared to have come
} down on you.
} The followups IMHO were starting to drift into
} critiques of Marilyn whomever
} and her "crap Journalism" and I was trying to nip
} that direction of
} critique in the bud. However, I see I had the
} opposite effect. Oh well.....
} As Dorrance said, everyone can use a grin
} occassionally.
}
} Nestor
}
----------------------------------------------------------------------------
---------------------}
} Nestor
} I don't see any reason why we could not discuss the
} quality of somebody's
} work even if it's a journalist with former (?) high
} IQ. Is it prohibited
} to discuss people's work with high IQ on this
} ListServer? Sergey
}
----------------------------------------------------------------------------
----------------
}
} 05:10 PM 7/26/2005
} Sergey
} A honest discussion or even disagreement about a
} subject or work which is
} microscopy
} related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally
} disparage any person or
} organization on the Listserver. This rule applies
} equally to Journalists
} as well as Microscopists. Please review the rules
} which you received upon subscription.
}
{http://microscopy.com/MicroscopyListserver/Rules.html} http://microscopy.com
/MicroscopyListserver/Rules.html
} Presumably a person of hi IQ has the ability to
} distinguish between these two.
} Nestor
} Your Friendly Neighborhood SysOp
}
----------------------------------------------------------------------------
---------------------
}
} 7/26/05
} My IQ is very low, Nestor, so I am electron
} microscopist for last 30
} years... Is against rules to say truth? You know,
} truth sometime hurts
} and may be politically incorrect... This forum
} becomes too much politically
} correct and heavily censured (yes, it's true), so I
} am loosing interest
} here... I had enough censorship and "rules" in
} USSR. But, I have to admit
} US is over performing USSR now, so I feel I am at
} home. Sergey
}
----------------------------------------------------------------------------
---------------------
}
} Date: Tue, 26 Jul 2005 21:02:29 -0500
} To: sryazant-at-ucla.edu
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} Subject: [Microscopy] Re: Discussion of high IQ vs
} Disparagement of a
}
} Sergy
}
} I don't care who you are or where your from, but I
} insist the rules which
} have been established for over a decade are
} followed. Since you believe
} you are above the rules so be it. You are welcome
} to go elsewhere.
}
} I have canceled your subscription to the Listserver.
}
} Nestor
}

==============================Original Headers==============================
3, 16 -- From Jane.LaGoy-at-bodycote.com Wed Aug 24 13:54:21 2005
3, 16 -- Received: from Exchange.BODYCOTE-IMT.COM (mail.bodycote-imt.com [12.30.23.178])
3, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j7OIsLVq028656
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3, 16 -- From: Jane LaGoy {Jane.LaGoy-at-bodycote.com}
3, 16 -- To: "Microscopy Listserver (E-mail)" {Microscopy-at-MSA.Microscopy.com}
3, 16 -- Cc: "'sryazant-at-ucla.edu'" {sryazant-at-ucla.edu}
3, 16 -- Subject: freedom of speech?
3, 16 -- Date: Wed, 24 Aug 2005 14:56:20 -0400
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3, 16 -- X-Mailer: Internet Mail Service (5.5.2657.72)
3, 16 -- Content-Type: text/plain;
3, 16 -- charset="iso-8859-1"
==============================End of - Headers==============================




From: r.sims-at-auckland.ac.nz
Date: Wed, 24 Aug 2005 15:49:46 -0500
Subject: [Microscopy] Sergey

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think Sergey got what he seemed to be asking for.

It was just absurd, as well as personally insulting to Nestor, to say that this
forum is "politically correct and heavily censured (sic)".

And he did say he was "loosing (sic) interest here".

I don't think that you, John, do either yourself or Sergey any favors by sending
this to the list. I don't think this should snowball.

I and most listers, belong to it because we want to participate in discussions
about microscopy, not listen to these little intrigues, vendettas, and exhibitions
of paranoia.

As I have said before, it's not an internet chat room.

rtch


} ----------------------------------------------- } } 7/26/05 } My IQ is
} very low, Nestor, so I am electron } microscopist for last 30 }
} years... Is against rules to say truth? You know, } truth sometime
} hurts } and may be politically incorrect... This forum } becomes too
} much politically } correct and heavily censured (yes, it's true), so I
} } am loosing interest } here... I had enough censorship and "rules"
} in } USSR. But, I have to admit } US is over performing USSR now, so I
} feel I am at } home. Sergey }
} ----------------------------------------------------------------------


--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


==============================Original Headers==============================
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13, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}
13, 27 -- Organization: Dept of Geology, Univ of Auckland
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From: DFORAN-at-ORA.FDA.GOV
Date: Wed, 24 Aug 2005 15:51:37 -0500
Subject: [Microscopy] freedom of speech?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor,

Please explain to the list why you removed Sergey. Thank you.

David A. Foran, chemist
Food and Drug Administration
Kansas City Elemental Analysis Lab
DFORAN-at-ORA.FDA.GOV
913-752-2170
913-752-2151 (fax)


-----Original Message-----
X-from: Jane.LaGoy-at-bodycote.com [mailto:Jane.LaGoy-at-bodycote.com]
Sent: Wednesday, August 24, 2005 1:56 PM
To: Foran, David A

IMHO it's a sad state of affairs that I am afraid to make any bold statement
for fear that I too will be censured, thrown off the Microscopy List and
denied any forum for appeal. Kind of mimics the current climate in our
national government....If this turns out to be my last communication, I want
to thank you all for your open and willing sharing of so much microscopy
knowledge. --Jane LaGoy

--- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:

} Dear John,
}
} Nestor has established a special filter to block all
} my messages not only
} to the ListServer but also emails addressed
} personally to him. He also
} denied me an explanation of what I did wrong.
} Therefore, I would like to
} ask the EM community to help me reinstate my name
} and membership at
} ListServer. If you feel comfortable, I would like
} to ask you to publish my
} letter on ListServer. I don't want to cause you any
} problems, so I suggest
} that if you decide to publish my letter, ask
} Nestor's permission first. I
} would also appreciate your opinion on this letter
} and would be happy to
} make corrections, clarifications, etc. Please feel
} free to tell me if this
} idea doesn't seem suitable and you would prefer not
} to participate. It's
} perfectly fine; I'll find other way to make my
} letter available to EM
} community. I would like to make it clear to my
} fellow microscopists that
} they may be treated in a similar way on ListServer
} if something is not
} done. For this reason, I want to speak directly to
} the people on the
} ListServer, not just Nestor. I also sincerely want
} clarification on what I
} did wrong and why I was boycotted in such harsh way.
} Thanks for your
} understanding and help. Sergey.
}
==============================================================
}
} Open letter to EM community:
}
}
} August 23, 2005
}
} Dear colleagues,
}
} It will soon be one month since I was disconnected
} from the EM
} community. I am asking for you to support
} reinstatement of my name and membership.
}
} As many of you are aware, Nestor cancelled my
} subscription to the
} Microscopy ListServer without a sufficiently clear
} explanation as to what I
} did wrong to the degree that cancellation of my
} membership was necessary. I
} didn't start the thread in question or participate
} in the thread, but
} commented only on the direction of the thread.
}
} After my membership was cancelled, I sent a private
} letter to Nestor asking
} him to explain in detail what was wrong in my recent
} postings, so I could
} adjust my behavior accordingly. He declined to
} answer my questions and
} immediately after installed a filter to block my
} messages. Since I lost
} the ability to communicate with Nestor directly, I
} am asking for your
} support in helping me to reinstate my membership at
} the EM ListServer.
}
} Many of you sent me copies of your emails to the
} ListServer asking Nestor
} to reinstate my membership. Many thanks for that
} support. I sincerely
} believe that a public place, especially a scientific
} forum such as the
} Microscopy ListServer, should not be ruled by any
} single person. Decisions
} should be made based on agreed upon principles that
} are established by the
} EM community. Please help me to reinstate my name
} and membership at ListServer.
}
} Thanks for your support. Sergey
}
}
} Here is the correspondence between Nestor and me
} that was on the ListServer
} and which presumably led to my membership
} cancellation. The most recent
} emails are on top. For simplicity's sake, I deleted
} duplicate email repeats
} and most headers. The original messages may be
} obtained from me or through ListServer:
}
} Tue Jul 26 11:51:32 2005
} I apologize to the list for posting Marilyn vos
} Savant's statement on
} microscopy. I just wanted to share the article
} because I thought it was
} humorous...I shall refrain from such postings.
} best, Beth
}
----------------------------------------------------------------------------
---------------------
} 7/26/2005
} Beth & everyone else
}
} There was no problem with Beth's initial posting
} and no apology was
} needed here Beth. Sorry if I appeared to have come
} down on you.
} The followups IMHO were starting to drift into
} critiques of Marilyn whomever
} and her "crap Journalism" and I was trying to nip
} that direction of
} critique in the bud. However, I see I had the
} opposite effect. Oh well.....
} As Dorrance said, everyone can use a grin
} occassionally.
}
} Nestor
}
----------------------------------------------------------------------------
---------------------}
} Nestor
} I don't see any reason why we could not discuss the
} quality of somebody's
} work even if it's a journalist with former (?) high
} IQ. Is it prohibited
} to discuss people's work with high IQ on this
} ListServer? Sergey
}
----------------------------------------------------------------------------
----------------
}
} 05:10 PM 7/26/2005
} Sergey
} A honest discussion or even disagreement about a
} subject or work which is
} microscopy
} related (even tangentially) is not prohibited.
}
} However, it is against the rules to intentionally
} disparage any person or
} organization on the Listserver. This rule applies
} equally to Journalists
} as well as Microscopists. Please review the rules
} which you received upon subscription.
}
{http://microscopy.com/MicroscopyListserver/Rules.html} http://microscopy.com
/MicroscopyListserver/Rules.html
} Presumably a person of hi IQ has the ability to
} distinguish between these two.
} Nestor
} Your Friendly Neighborhood SysOp
}
----------------------------------------------------------------------------
---------------------
}
} 7/26/05
} My IQ is very low, Nestor, so I am electron
} microscopist for last 30
} years... Is against rules to say truth? You know,
} truth sometime hurts
} and may be politically incorrect... This forum
} becomes too much politically
} correct and heavily censured (yes, it's true), so I
} am loosing interest
} here... I had enough censorship and "rules" in
} USSR. But, I have to admit
} US is over performing USSR now, so I feel I am at
} home. Sergey
}
----------------------------------------------------------------------------
---------------------
}
} Date: Tue, 26 Jul 2005 21:02:29 -0500
} To: sryazant-at-ucla.edu
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
} Subject: [Microscopy] Re: Discussion of high IQ vs Disparagement of a
}
} Sergy
}
} I don't care who you are or where your from, but I
} insist the rules which
} have been established for over a decade are
} followed. Since you believe
} you are above the rules so be it. You are welcome
} to go elsewhere.
}
} I have canceled your subscription to the Listserver.
}
} Nestor
}

==============================Original Headers==============================
3, 16 -- From Jane.LaGoy-at-bodycote.com Wed Aug 24 13:54:21 2005 3, 16 --
Received: from Exchange.BODYCOTE-IMT.COM (mail.bodycote-imt.com
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3, 16 -- To: "Microscopy Listserver (E-mail)"
{Microscopy-at-MSA.Microscopy.com} 3, 16 -- Cc: "'sryazant-at-ucla.edu'"
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==============================Original Headers==============================
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13, 16 -- Received: from wallsand-pub.fda.gov (wallsand-pub.fda.gov [150.148.0.31])
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13, 16 -- From: "Foran, David A" {DFORAN-at-ORA.FDA.GOV}
13, 16 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
13, 16 -- Subject: FW: [Microscopy] freedom of speech?
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From: zaluzec-at-microscopy.com
Date: Wed, 24 Aug 2005 16:13:35 -0500
Subject: [Microscopy] Administrivia: Listserver Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

As many of you know, an individual's subscription was canceled to
the Listserver
in July , as per the rules of the Microscopy Listserver to which
everyone must
comply in order to maintain their subscription. An unverifiable message was
recently forwarded by a third party to the listserver concerning this matter.

For the record, the subscriber was informed of the cancellation and
instructed that he could apply
for a new subscription after an appointed period of time. There
have been a total of six
individuals to whom this restriction has been applied in the last 12
YEARS of operation
of the Microscopy Listserver. Of the six, all but two have been
fully reinstated and
they have, since that time, compiled with the rules of the
Microscopy Listserver.
The individual in question, should he so choose, will be given the
same opportunity.

I should also note for clarification, that contrary to the
unverifiable statements purported to be
from this individual, there are no special filters placed upon him personally,
only those imposed upon all non-subscribers and SPAM/UCE sources
by automated server
software.

I will remind you that the Listserver is a subscripton based forum
having well defined
and professional purposes, which has rules concerning its use as well as
membership. It is moderated and is monitored for complaince with its rules
with reasonable decorum and professionalism expected at all times
by subscribers.
There are numerous unmoderated public chat rooms which are
available to anyone that is interested in participating in
unbridled open ended public venues.

If you wish to review the Microscopy Listserver Rules, you may find
them on-line at
http://www.microscopy.com/MicroscopyListserver.

Anyone that feels that the Listerver rules are too restrictive for their liking
is welcome to unsubscribe at any time. The electronic forms for this
can also be found
at the above WWW site.

There is no purpose in further discussion on this matter, as
Administrator/SysOp of the
Microscopy Listserver, this decision was mine and mine alone, and
it is final. You
are welcome to address comments to me personally off-line should you
so desire.

I consider this thread closed and suggest that this forum return to
questions/discussions
concerning its purpose i.e. Microscopy & Microanalysis.

Nestor
The Microscopy SysOp


==============================Original Headers==============================
11, 15 -- From zaluzec-at-microscopy.com Wed Aug 24 16:13:35 2005
11, 15 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3])
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11, 15 -- Received: from [146.139.72.105] (aem105.amc.anl.gov [146.139.72.105])
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11, 15 -- Message-Id: {p06110404bf326550fbf7-at-[146.139.72.105]}
11, 15 -- Date: Wed, 24 Aug 2005 16:13:33 -0500
11, 15 -- To: microscopy-at-microscopy.com
11, 15 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
11, 15 -- Subject: Administrivia: Listserver Rules
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==============================End of - Headers==============================




From: hoffpajo-at-yahoo.com
Date: Wed, 24 Aug 2005 20:14:44 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

And right you were.

--- "r. williams" {s2kdude-at-pacbell.net} wrote:

} i predict Nestor will say nothing but a re-iteration
} of his rules.
}
} --- hoffpajo-at-yahoo.com wrote:
}
} }
} }
} }
} }
}
----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The
} } Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
----------------------------------------------------------------------------
} }
} }
} }
} } --- Sergey Ryazantsev {sryazant-at-ucla.edu} wrote:
} }
} } } Dear John,
} } }
} } } Nestor has established a special filter to block
} } all
} } } my messages not only
} } } to the ListServer but also emails addressed
} } } personally to him. He also
} } } denied me an explanation of what I did wrong.
} } } Therefore, I would like to
} } } ask the EM community to help me reinstate my
} name
} } } and membership at
} } } ListServer. If you feel comfortable, I would
} like
} } } to ask you to publish my
} } } letter on ListServer. I don't want to cause you
} } any
} } } problems, so I suggest
} } } that if you decide to publish my letter, ask
} } } Nestor's permission first. I
} } } would also appreciate your opinion on this
} letter
} } } and would be happy to
} } } make corrections, clarifications, etc. Please
} } feel
} } } free to tell me if this
} } } idea doesn't seem suitable and you would prefer
} } not
} } } to participate. It's
} } } perfectly fine; I'll find other way to make my
} } } letter available to EM
} } } community. I would like to make it clear to my
} } } fellow microscopists that
} } } they may be treated in a similar way on
} ListServer
} } } if something is not
} } } done. For this reason, I want to speak directly
} } to
} } } the people on the
} } } ListServer, not just Nestor. I also sincerely
} } want
} } } clarification on what I
} } } did wrong and why I was boycotted in such harsh
} } way.
} } } Thanks for your
} } } understanding and help. Sergey.
} } }
} } }
} }
}
==============================================================
} } }
} } } Open letter to EM community:
} } }
} } }
}
} }
} } }
} August
} } } 23, 2005
} } }
} } } Dear colleagues,
} } }
} } } It will soon be one month since I was
} disconnected
} } } from the EM
} } } community. I am asking for you to support
} } } reinstatement of my name and
} } } membership.
} } }
} } } As many of you are aware, Nestor cancelled my
} } } subscription to the
} } } Microscopy ListServer without a sufficiently
} clear
} } } explanation as to what I
} } } did wrong to the degree that cancellation of my
} } } membership was necessary. I
} } } didn't start the thread in question or
} participate
} } } in the thread, but
} } } commented only on the direction of the thread.
} } }
} } } After my membership was cancelled, I sent a
} } private
} } } letter to Nestor asking
} } } him to explain in detail what was wrong in my
} } recent
} } } postings, so I could
} } } adjust my behavior accordingly. He declined to
} } } answer my questions and
} } } immediately after installed a filter to block my
} } } messages. Since I lost
} } } the ability to communicate with Nestor directly,
} I
} } } am asking for your
} } } support in helping me to reinstate my membership
} } at
} } } the EM ListServer.
} } }
} } } Many of you sent me copies of your emails to the
} } } ListServer asking Nestor
} } } to reinstate my membership. Many thanks for
} that
} } } support. I sincerely
} } } believe that a public place, especially a
} } scientific
} } } forum such as the
} } } Microscopy ListServer, should not be ruled by
} any
} } } single person. Decisions
} } } should be made based on agreed upon principles
} } that
} } } are established by the
} } } EM community. Please help me to reinstate my
} name
} } } and membership at
} } } ListServer.
} } }
} } } Thanks for your support. Sergey
} } }
} } }
} } } Here is the correspondence between Nestor and me
} } } that was on the ListServer
} } } and which presumably led to my membership
} } } cancellation. The most recent
} } } emails are on top. For simplicity's sake, I
} } deleted
} } } duplicate email repeats
} } } and most headers. The original messages may be
} } } obtained from me or through
} } } ListServer:
} } }
} } } Tue Jul 26 11:51:32 2005
} } } I apologize to the list for posting Marilyn vos
} } } Savant's statement on
} } } microscopy. I just wanted to share the article
} } } because I thought it was
} } } humorous...I shall refrain from such postings.
} } } best,
} } } Beth
} } }
} } }
} }
}
------------------------------------------------------------------------------------------------------------------
} } } 7/26/2005
} } } Beth & everyone else
} } }
} } } There was no problem with Beth's initial
} posting
} } } and no apology was
} } } needed here Beth. Sorry if I appeared to have
} } come
} } } down on you.
} } } The followups IMHO were starting to drift into
} } } critiques of Marilyn whomever
} } } and her "crap Journalism" and I was trying to
} nip
} } } that direction of
} } } critique in the bud. However, I see I had the
} } } opposite effect. Oh well.....
} } } As Dorrance said, everyone can use a grin
} } } occassionally .
} } }
} } } Nestor
} } }
} } }
} }
}
---------------------------------------------------------------------------------------------------
} } }
} } } Nestor
} } } I don't see any reason why we could not discuss
} } the
} } } quality of somebody's
} } } work even if it's a journalist with former (?)
} } high
} } } IQ. Is it prohibited
} } } to discuss people's work with high IQ on this
} } } ListServer? Sergey
}
=== message truncated ===


__________________________________________________
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==============================Original Headers==============================
5, 20 -- From hoffpajo-at-yahoo.com Wed Aug 24 20:14:44 2005
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5, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 20 -- Subject: Re: [Microscopy] Re: Discussion of high IQ vs Disparagement of a
5, 20 -- To: "r. williams" {s2kdude-at-pacbell.net}
5, 20 -- Cc: microscopy-at-microscopy.com
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From: hoffpajo-at-yahoo.com
Date: Wed, 24 Aug 2005 20:28:47 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This will be the last time I respond to your your
email.
I have been to Europe more times than you have been to
the US. I am a world traveler. In fact I will be in
Nice France next week attending a medical conference.
Care to hash it out there. I can give you my exact
hotel and dates I will be there.
John Hoffpauir

--- Matthias Mörgelin {Matthias.Morgelin-at-med.lu.se}
wrote:

} I will neither block you email address or simply
} delete your mails, as you propose, this is not
} necessary, I will just ignore your little rooster
} thing as probably many other grown-up people in this
} forum will do. This is my last response to your
} personal war against the listserver.
} Best regards from Sweden (if you know at all where
} this land is located)
}
} ----- Original Message -----
} From: john hoffpauir {hoffpajo-at-yahoo.com}
} Date: Wednesday, August 24, 2005 10:30 pm
} Subject: Re: [Microscopy] Re: Discussion of high IQ
} vs Disparagement of a
}
} } i am not on an ego trip, but it sure sounds like
} you
} } have some issues to work out. if you don't like
} what i
} } have to say, block my email address or simply
} delete
} } them. try to be a little more open minded.
} } john hoffpauir
} }
} }
} }
} } --- Matthias Mörgelin
} {Matthias.Morgelin-at-med.lu.se}
} } wrote:
} }
} } } ..."so I am loosing interest here"...etc
} } } blablabla.!!!
} } }
} } } This forum has not been established for persons
} like
} } } you to express your very own small personal
} ego-trip
} } } things all the time. Sometimes I really admire
} } } Nestor for his neverending positive attitude
} towards
} } } guys like you! Personally I would have blocked
} you
} } } much earler!
} } }
} } }
} } } ----- Original Message -----
} } } From: hoffpajo-at-yahoo.com
} } } Date: Wednesday, August 24, 2005 7:46 pm
} } } Subject: [Microscopy] Re: Discussion of high IQ
} vs
} } } Disparagement of a
} } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
}
-------------------------------------------------------------------
} } } } ---------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of
} } } } AmericaTo Subscribe/Unsubscribe --
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserverOn-Line
} } } Help
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} } } }
} } }
} }
}
----------------------------------------------------------------
} } } }
} } } }
} } } }
} } } } --- Sergey Ryazantsev {sryazant-at-ucla.edu}
} wrote:
} } } }
} } } } } Dear John,
} } } } }
} } } } } Nestor has established a special filter to
} block
} } } all
} } } } } my messages not only
} } } } } to the ListServer but also emails addressed
} } } } } personally to him. He also
} } } } } denied me an explanation of what I did
} wrong.
} } } } } Therefore, I would like to
} } } } } ask the EM community to help me reinstate my
} } } name
} } } } } and membership at
} } } } } ListServer. If you feel comfortable, I
} would
} } } like
} } } } } to ask you to publish my
} } } } } letter on ListServer. I don't want to cause
} you
} } } any
} } } } } problems, so I suggest
} } } } } that if you decide to publish my letter, ask
} } } } } Nestor's permission first. I
} } } } } would also appreciate your opinion on this
} } } letter
} } } } } and would be happy to
} } } } } make corrections, clarifications, etc.
} Please
} } } feel
} } } } } free to tell me if this
} } } } } idea doesn't seem suitable and you would
} prefer
} } } not
} } } } } to participate. It's
} } } } } perfectly fine; I'll find other way to make
} my
} } } } } letter available to EM
} } } } } community. I would like to make it clear to
} my
} } } } } fellow microscopists that
} } } } } they may be treated in a similar way on
} } } ListServer
} } } } } if something is not
} } } } } done. For this reason, I want to speak
} directly
} } } to
} } } } } the people on the
} } } } } ListServer, not just Nestor. I also
} sincerely
} } } want
} } } } } clarification on what I
} } } } } did wrong and why I was boycotted in such
} harsh
} } } way.
} } } } } Thanks for your
} } } } } understanding and help. Sergey.
} } } } }
} } } } }
} } } }
} } }
} }
}
==============================================================
} } } } }
} } } } } Open letter to EM community:
} } } } }
} } } } }
}
} } }
} } } } }
} } } August
} } } } } 23, 2005
} } } } }
} } } } } Dear colleagues,
} } } } }
} } } } } It will soon be one month since I was
} } } disconnected
} } } } } from the EM
} } } } } community. I am asking for you to support
} } } } } reinstatement of my name and
} } } } } membership.
} } } } }
} } } } } As many of you are aware, Nestor cancelled
} my
} } } } } subscription to the
} } } } } Microscopy ListServer without a sufficiently
} } } clear
} } } } } explanation as to what I
} } } } } did wrong to the degree that cancellation of
} my
} } } } } membership was necessary. I
} } } } } didn't start the thread in question or
} } } participate
} } } } } in the thread, but
} } } } } commented only on the direction of the
} thread.
} } } } }
} } } } } After my membership was cancelled, I sent a
} } } private
} } } } } letter to Nestor asking
} } } } } him to explain in detail what was wrong in
} my
} } } recent
} } } } } postings, so I could
} } } } } adjust my behavior accordingly. He declined
} to
} } } } } answer my questions and
} } } } } immediately after installed a filter to
} block my
} } } } } messages. Since I lost
} } } } } the ability to communicate with Nestor
} directly,
} } } I
} } } } } am asking for your
} } } } } support in helping me to reinstate my
} membership
} } } at
} } } } } the EM ListServer.
} } } } }
}
=== message truncated ===


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==============================Original Headers==============================
5, 20 -- From hoffpajo-at-yahoo.com Wed Aug 24 20:28:47 2005
5, 20 -- Received: from web50202.mail.yahoo.com (web50202.mail.yahoo.com [206.190.38.43])
5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j7P1Skg2008543
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5, 20 -- Date: Wed, 24 Aug 2005 18:28:46 -0700 (PDT)
5, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
5, 20 -- Subject: Re: [Microscopy] Re: Discussion of high IQ vs Disparagement of a
5, 20 -- To: Matthias "Mörgelin" {Matthias.Morgelin-at-med.lu.se}
5, 20 -- Cc: microscopy-at-microscopy.com
5, 20 -- In-Reply-To: {1afb3801af6965.1af69651afb380-at-net.lu.se}
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==============================End of - Headers==============================




From: hoffpajo-at-yahoo.com
Date: Wed, 24 Aug 2005 20:44:16 -0500
Subject: [Microscopy] Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hmmmm is this disparagement? Personal I don't like
fruitcake.

--- Matthias Mörgelin {Matthias.Morgelin-at-med.lu.se}
wrote:

} interesting which fruitcakes you can meet on the
} net. some of them even seem to be psycologists
}
} ----- Original Message -----
} From: john hoffpauir {hoffpajo-at-yahoo.com}
} Date: Wednesday, August 24, 2005 10:30 pm
} Subject: Re: [Microscopy] Re: Discussion of high IQ
} vs Disparagement of a
}
} } i am not on an ego trip, but it sure sounds like
} you
} } have some issues to work out. if you don't like
} what i
} } have to say, block my email address or simply
} delete
} } them. try to be a little more open minded.
} } john hoffpauir
} }
} }
} }
} } --- Matthias Mörgelin
} {Matthias.Morgelin-at-med.lu.se}
} } wrote:
} }
} } } ..."so I am loosing interest here"...etc
} } } blablabla.!!!
} } }
} } } This forum has not been established for persons
} like
} } } you to express your very own small personal
} ego-trip
} } } things all the time. Sometimes I really admire
} } } Nestor for his neverending positive attitude
} towards
} } } guys like you! Personally I would have blocked
} you
} } } much earler!
} } }
} } }
} } } ----- Original Message -----
} } } From: hoffpajo-at-yahoo.com
} } } Date: Wednesday, August 24, 2005 7:46 pm
} } } Subject: [Microscopy] Re: Discussion of high IQ
} vs
} } } Disparagement of a
} } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
}
-------------------------------------------------------------------
} } } } ---------
} } } } The Microscopy ListServer -- CoSponsor: The
} } } Microscopy Society of
} } } } AmericaTo Subscribe/Unsubscribe --
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserverOn-Line
} } } Help
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} } } }
} } }
} }
}
----------------------------------------------------------------
} } } }
} } } }
} } } }
} } } } --- Sergey Ryazantsev {sryazant-at-ucla.edu}
} wrote:
} } } }
} } } } } Dear John,
} } } } }
} } } } } Nestor has established a special filter to
} block
} } } all
} } } } } my messages not only
} } } } } to the ListServer but also emails addressed
} } } } } personally to him. He also
} } } } } denied me an explanation of what I did
} wrong.
} } } } } Therefore, I would like to
} } } } } ask the EM community to help me reinstate my
} } } name
} } } } } and membership at
} } } } } ListServer. If you feel comfortable, I
} would
} } } like
} } } } } to ask you to publish my
} } } } } letter on ListServer. I don't want to cause
} you
} } } any
} } } } } problems, so I suggest
} } } } } that if you decide to publish my letter, ask
} } } } } Nestor's permission first. I
} } } } } would also appreciate your opinion on this
} } } letter
} } } } } and would be happy to
} } } } } make corrections, clarifications, etc.
} Please
} } } feel
} } } } } free to tell me if this
} } } } } idea doesn't seem suitable and you would
} prefer
} } } not
} } } } } to participate. It's
} } } } } perfectly fine; I'll find other way to make
} my
} } } } } letter available to EM
} } } } } community. I would like to make it clear to
} my
} } } } } fellow microscopists that
} } } } } they may be treated in a similar way on
} } } ListServer
} } } } } if something is not
} } } } } done. For this reason, I want to speak
} directly
} } } to
} } } } } the people on the
} } } } } ListServer, not just Nestor. I also
} sincerely
} } } want
} } } } } clarification on what I
} } } } } did wrong and why I was boycotted in such
} harsh
} } } way.
} } } } } Thanks for your
} } } } } understanding and help. Sergey.
} } } } }
} } } } }
} } } }
} } }
} }
}
==============================================================
} } } } }
} } } } } Open letter to EM community:
} } } } }
} } } } }
}
} } }
} } } } }
} } } August
} } } } } 23, 2005
} } } } }
} } } } } Dear colleagues,
} } } } }
} } } } } It will soon be one month since I was
} } } disconnected
} } } } } from the EM
} } } } } community. I am asking for you to support
} } } } } reinstatement of my name and
} } } } } membership.
} } } } }
} } } } } As many of you are aware, Nestor cancelled
} my
} } } } } subscription to the
} } } } } Microscopy ListServer without a sufficiently
} } } clear
} } } } } explanation as to what I
} } } } } did wrong to the degree that cancellation of
} my
} } } } } membership was necessary. I
} } } } } didn't start the thread in question or
} } } participate
} } } } } in the thread, but
} } } } } commented only on the direction of the
} thread.
} } } } }
} } } } } After my membership was cancelled, I sent a
} } } private
} } } } } letter to Nestor asking
} } } } } him to explain in detail what was wrong in
} my
} } } recent
} } } } } postings, so I could
} } } } } adjust my behavior accordingly. He declined
} to
} } } } } answer my questions and
} } } } } immediately after installed a filter to
} block my
} } } } } messages. Since I lost
} } } } } the ability to communicate with Nestor
} directly,
} } } I
} } } } } am asking for your
} } } } } support in helping me to reinstate my
} membership
} } } at
} } } } } the EM ListServer.
} } } } }
} } } } } Many of you sent me copies of your emails to
} the
} } } } } ListServer asking Nestor
} } } } } to reinstate my membership. Many thanks for
} } } that
} } } } } support. I sincerely
}
=== message truncated ===


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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==============================Original Headers==============================
6, 20 -- From hoffpajo-at-yahoo.com Wed Aug 24 20:44:16 2005
6, 20 -- Received: from web50208.mail.yahoo.com (web50208.mail.yahoo.com [206.190.38.49])
6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j7P1iGfp016565
6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 24 Aug 2005 20:44:16 -0500
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6, 20 -- Message-ID: {20050825014416.39420.qmail-at-web50208.mail.yahoo.com}
6, 20 -- Received: from [68.32.53.160] by web50208.mail.yahoo.com via HTTP; Wed, 24 Aug 2005 18:44:16 PDT
6, 20 -- Date: Wed, 24 Aug 2005 18:44:16 -0700 (PDT)
6, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
6, 20 -- Subject: Re: [Microscopy] Re: Discussion of high IQ vs Disparagement of a
6, 20 -- To: Matthias "Mörgelin" {Matthias.Morgelin-at-med.lu.se}
6, 20 -- Cc: microscopy-at-microscopy.com
6, 20 -- In-Reply-To: {1af85be1af6797.1af67971af85be-at-net.lu.se}
6, 20 -- MIME-Version: 1.0
6, 20 -- Content-Type: text/plain; charset=iso-8859-1
6, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: hoffpajo-at-yahoo.com
Date: Wed, 24 Aug 2005 20:48:55 -0500
Subject: [Microscopy] Re: Discussion of high IQ vs Disparagement of a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It maybe 1500 miles. I have never been to Stockholm. I
it however over 3000 miles to Nice from Philadelphia,
but I am still going.
John

--- Mike O'Keefe {MAOKeefe-at-lbl.gov} wrote:

} Isn't it, like, 1500 miles from Nice to Stockholm?
}
} ----- Original Message -----
} From: hoffpajo-at-yahoo.com
} Date: Wednesday, August 24, 2005 6:30 pm
} Subject: [Microscopy] Discussion of high IQ vs
} Disparagement of a
}
} }
} }
} }
} }
}
--------------------------------------------------------------------
} } --------
} } The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of
} } AmericaTo Subscribe/Unsubscribe --
} }
}
http://www.microscopy.com/MicroscopyListserverOn-Line
} Help
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html-------------
} }
}
---------------------------------------------------------------
} }
} } This will be the last time I respond to your your
} } email.
} } I have been to Europe more times than you have
} been to
} } the US. I am a world traveler. In fact I will be
} in
} } Nice France next week attending a medical
} conference.
} } Care to hash it out there. I can give you my exact
} } hotel and dates I will be there.
} } John Hoffpauir
} }
} } --- Matthias Mörgelin
} {Matthias.Morgelin-at-med.lu.se}
} } wrote:
} }
} } } I will neither block you email address or simply
} } } delete your mails, as you propose, this is not
} } } necessary, I will just ignore your little
} rooster
} } } thing as probably many other grown-up people in
} this
} } } forum will do. This is my last response to your
} } } personal war against the listserver.
} } } Best regards from Sweden (if you know at all
} where
} } } this land is located)
} } }
} } } ----- Original Message -----
} } } From: john hoffpauir {hoffpajo-at-yahoo.com}
} } } Date: Wednesday, August 24, 2005 10:30 pm
} } } Subject: Re: [Microscopy] Re: Discussion of
} high IQ
} } } vs Disparagement of a
} } }
} } } } i am not on an ego trip, but it sure sounds
} like
} } } you
} } } } have some issues to work out. if you don't
} like
} } } what i
} } } } have to say, block my email address or simply
} } } delete
} } } } them. try to be a little more open minded.
} } } } john hoffpauir
} } } }
} } } }
} } } }
} } } } --- Matthias Mörgelin
} } } {Matthias.Morgelin-at-med.lu.se}
} } } } wrote:
} } } }
} } } } } ..."so I am loosing interest here"...etc
} } } } } blablabla.!!!
} } } } }
} } } } } This forum has not been established for
} persons
} } } like
} } } } } you to express your very own small personal
} } } ego-trip
} } } } } things all the time. Sometimes I really
} admire
} } } } } Nestor for his neverending positive attitude
} } } towards
} } } } } guys like you! Personally I would have
} blocked
} } } you
} } } } } much earler!
} } } } }
} } } } }
} } } } } ----- Original Message -----
} } } } } From: hoffpajo-at-yahoo.com
} } } } } Date: Wednesday, August 24, 2005 7:46 pm
} } } } } Subject: [Microscopy] Re: Discussion of
} high IQ
} } } vs
} } } } } Disparagement of a
} } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } } }
} } } } }
} } } }
} } }
} }
}
-------------------------------------------------------------------
} } } } } } ---------
} } } } } } The Microscopy ListServer -- CoSponsor:
} The
} } } } } Microscopy Society of
} } } } } } AmericaTo Subscribe/Unsubscribe --
} } } } } }
} } } } }
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserverOn-Line
} } } } } Help
} } } } } }
} } } } }
} } } }
} } }
} }
}
http://www.microscopy.com/MicroscopyListserver/FAQ.html------------
} } } } } }
} } } } }
} } } }
} } }
} }
}
----------------------------------------------------------------
} } } } } }
} } } } } }
} } } } } }
} } } } } } --- Sergey Ryazantsev {sryazant-at-ucla.edu}
} } } wrote:
} } } } } }
} } } } } } } Dear John,
} } } } } } }
} } } } } } } Nestor has established a special filter
} to
} } } block
} } } } } all
} } } } } } } my messages not only
} } } } } } } to the ListServer but also emails
} addressed
} } } } } } } personally to him. He also
} } } } } } } denied me an explanation of what I did
} } } wrong.
} } } } } } } Therefore, I would like to
} } } } } } } ask the EM community to help me
} reinstate my
} } } } } name
} } } } } } } and membership at
} } } } } } } ListServer. If you feel comfortable, I
} } } would
} } } } } like
} } } } } } } to ask you to publish my
} } } } } } } letter on ListServer. I don't want to
} cause
} } } you
} } } } } any
} } } } } } } problems, so I suggest
} } } } } } } that if you decide to publish my letter,
} ask
} } } } } } } Nestor's permission first. I
} } } } } } } would also appreciate your opinion on
} this
} } } } } letter
} } } } } } } and would be happy to
} } } } } } } make corrections, clarifications, etc.
} } } Please
} } } } } feel
} } } } } } } free to tell me if this
} } } } } } } idea doesn't seem suitable and you would
} } } prefer
} } } } } not
} } } } } } } to participate. It's
} } } } } } } perfectly fine; I'll find other way to
} make
} } } my
} } } } } } } letter available to EM
} } } } } } } community. I would like to make it
} clear to
} } } my
} } } } } } } fellow microscopists that
} } } } } } } they may be treated in a similar way on
} } } } } ListServer
} } } } } } } if something is not
} } } } } } } done. For this reason, I want to speak
} } } directly
} } } } } to
} } } } } } } the people on the
} } } } } } } ListServer, not just Nestor. I also
} } } sincerely
} } } } } want
}
=== message truncated ===




____________________________________________________
Start your day with Yahoo! - make it your home page
http://www.yahoo.com/r/hs


==============================Original Headers==============================
7, 20 -- From hoffpajo-at-yahoo.com Wed Aug 24 20:48:54 2005
7, 20 -- Received: from web50207.mail.yahoo.com (web50207.mail.yahoo.com [206.190.38.48])
7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id j7P1msAs024388
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7, 20 -- Received: from [68.32.53.160] by web50207.mail.yahoo.com via HTTP; Wed, 24 Aug 2005 18:48:54 PDT
7, 20 -- Date: Wed, 24 Aug 2005 18:48:54 -0700 (PDT)
7, 20 -- From: john hoffpauir {hoffpajo-at-yahoo.com}
7, 20 -- Subject: Re: [Microscopy] Discussion of high IQ vs Disparagement of a
7, 20 -- To: "Mike O'Keefe" {MAOKeefe-at-lbl.gov}
7, 20 -- Cc: microscopy-at-microscopy.com
7, 20 -- In-Reply-To: {335f1e3324e0.3324e0335f1e-at-lbl.gov}
7, 20 -- MIME-Version: 1.0
7, 20 -- Content-Type: text/plain; charset=iso-8859-1
7, 20 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: uti-at-direcpc.com
Date: Thu, 25 Aug 2005 08:00:02 -0500
Subject: [Microscopy] Re: viaWWW: Jeol JSM 35-CF scan generator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Henrik,

There are several modifications for the JEOL-35, you need to know which
microscope you have. One type has a connection in the back, labeled as
JA-2, which is a 9-pin Amphenol connector. The other type has a fairly
large round connector for external beam control.

Look for diagrams in the book for wiring of that connector. I have diagram
for Amphenol connector only. Let me know if you need them.
Good luck,
Vlad



At 07:33 AM 8/23/2005 -0500, Henrik.Kaker-at-guest.arnes.si wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



==============================Original Headers==============================
11, 19 -- From uti-at-direcpc.com Thu Aug 25 08:00:02 2005
11, 19 -- Received: from a34-mta02.direcway.com (a34-mta02.direcpc.com [66.82.4.91])
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From: hoffpajo-at-yahoo.com
Date: Thu, 25 Aug 2005 09:30:30 -0500
Subject: [Microscopy] leaving now

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

ok, I have finally had enough of the personal attacks
I have received. I will no longer be a member of this
listserver.
Those of you that have issues with me start your party
now.
John Hoffpauir



____________________________________________________
Start your day with Yahoo! - make it your home page
http://www.yahoo.com/r/hs


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From: pekysar-at-ucdavis.edu
Date: Thu, 25 Aug 2005 11:37:22 -0500
Subject: [Microscopy] Service for Balzers 360M Freeze Fracture Machine

Contents Retrieved from Microscopy Listserver Archives
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Hi All,
We are trying to find someone to work on our Balzers 360M Freezed Fracture
machine. It is a "modernized" unit with a thin film monitor and rotary
shadow device. It was is good working order when it was "decommissioned"
about 4 years ago but has been sitting idle since then. We would like to
find
someone in the Sacramento/San Francisco area who can help us get this
intrument up and running and who we can call for future service. We would
also like to know about general reliability and parts availability.
Thanks,
Pat Kysar
UC Davis Medical Pathology
EM Lab
530-752-4701


==============================Original Headers==============================
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From: sghoshro-at-NMSU.Edu
Date: Thu, 25 Aug 2005 11:38:00 -0500
Subject: [Microscopy] SEM part needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We have an old Phillips 501B SEM in the Physics department and they are
looking for a photomultiplier tube (XP 2010/XP 1010). So if you have one
or know someone who has one and willing to sell it, then please let us
know. You can contact me offline.

Thanks a lot.

Soumitra

*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282

==============================Original Headers==============================
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From: dans-at-ameslab.gov
Date: Thu, 25 Aug 2005 12:07:42 -0500
Subject: [Microscopy] viaWWW: Looking for Used Dimpling unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dans-at-ameslab.gov) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, August 25, 2005 at 09:51:11
---------------------------------------------------------------------------

Email: dans-at-ameslab.gov
Name: Dan Shechtman

Organization: Ames Lab.

Title-Subject: [Filtered] Used Dimpling unit

Question: I am interested in purchasing a used Dimpling unit, VCR or Gatan.
Please send information including asking price, and if you can, a picture.

---------------------------------------------------------------------------

==============================Original Headers==============================
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6, 12 -- Subject: viaWWW: Looking for Used Dimpling unit
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From: rothbardd-at-netscape.net
Date: Thu, 25 Aug 2005 13:11:16 -0500
Subject: [Microscopy] National Museum of Health and Medicine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was concerned, as some you may have been, about the impact of the closure of the Walter Reed Medical Center in Washington, DC on the future of the Billings Microscope Collection housed in the National Musuem of Health and Medicine there. The museum director told me today that specific language in the BRAC closure order preserves the Museum facility. The museum's website is www.nmhm.washingtondc.museum

David Rothbard
US Bureau of Engraving and Printing

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From: ahlst007-at-umn.edu
Date: Thu, 25 Aug 2005 13:16:46 -0500
Subject: [Microscopy] Re: SEM part needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Soumitra,

Try Alex Greene of Electron Optics Repair and Installation. He bought our
old Philips SEM 500 a few years ago, and may have its or other similar model
photomultiplier tubes available. Tho this info is a few years old, you may
be able to reach him at:

ablue-at-io.com

or: (512)282-5507

Gib
--
Gib Ahlstrand, Scientist
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-2785 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/

} Dear Colleagues,
}
} We have an old Phillips 501B SEM in the Physics department and they are
} looking for a photomultiplier tube (XP 2010/XP 1010). So if you have one
} or know someone who has one and willing to sell it, then please let us
} know. You can contact me offline.
}
} Thanks a lot.
}
} Soumitra
}
} *************************************************************
} Soumitra Ghoshroy
} College Associate Professor, Biology
} Director, Electron Microscopy Lab
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3268 (office), 646-3283 (lab)
} Fax: 505-646-3282




==============================Original Headers==============================
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From: rothbardd-at-netscape.net
Date: Thu, 25 Aug 2005 13:25:31 -0500
Subject: [Microscopy] Re: National Museum of Health and Medicine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The link works for me. Under Exhibits-Permanent there is a short note about the collection. There are some electron microscopes, but they were not well displayed when I was there last.

Ken Tiekotter {tiekotte-at-up.edu} wrote:

} David,
}
} Will the Billings collection have a website? The www.nmhm.....website states
} it is not in use.
}
} Regards,
} Ken
}
} _______________________________________
} Kenneth L. Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N Willamette Blvd.
} Portland, OR 97203 USA
}
} Tel.: 503.943.8861
} Email: tiekotte-at-up.edu
}
}
}
} On 8/25/05 11:11 AM, "rothbardd-at-netscape.net" {rothbardd-at-netscape.net}
} wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } I was concerned, as some you may have been, about the impact of the closure of
} } the Walter Reed Medical Center in Washington, DC on the future of the Billings
} } Microscope Collection housed in the National Musuem of Health and Medicine
} } there.  The museum director told me today that specific language in the BRAC
} } closure order preserves the Museum facility. The museum's website is
} } www.nmhm.washingtondc.museum
} }
} } David Rothbard
} } US Bureau of Engraving and Printing
} }
} } __________________________________________________________________
} } Switch to Netscape Internet Service.
} } As low as $9.95 a month -- Sign up today at http://isp.netscape.com/register
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} } ==============================Original Headers==============================
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}
}

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From: edelmare-at-muohio.edu
Date: Thu, 25 Aug 2005 14:02:44 -0500
Subject: [Microscopy] Re: viaWWW: digital SLR to a c-mount on a stereo microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul:

Mounting a digital SLR on a photo-port on a LM is very easy to
do - BUT I do not know of a digital SLR which uses a C-mount
specifically. The c-mounts are very small, digital SLR's, like film
SLR's use larger mounts like a nikon F-mount, Olympus O-mount,
Canon EF-mount, pentax KA-mount, or a more generic T-mount, or
other.

AND you will generally need to use some type of photo-eye
piece lens to project the image to the "film"/"sensor" plane of the
SLR


Your Leica may very well have an adapter on it for the C-
mounting which you will need to replace for a specific SLR body.

On 22 Aug 2005, at 17:56, pbarber-at-bu.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pbarber-at-bu.edu) from http://www.microscopy.com/MLFormMail.html on Monday, August 22, 2005 at 11:05:10
} ---------------------------------------------------------------------------
}
} Email: pbarber-at-bu.edu
} Name: Paul Barber
}
} Organization: Boston University
}
} Title-Subject: [Filtered] MListserver:
}
} Question: I would like to be able to attach a digital SLR (ie Nikon D100) to a c-mount on a stereo microscope like a Leica MZ9.5 or Nikon SMZ800. I've heard conflicting answers from product reps, some saying that it isn't possible, some saying it is? However, I have no experience at all
microscopy, but need to buy a system that has at least some photodocumentation ability. I don't need (and can't afford) a 4-5000 dollar imaging system. It seems like putting a digital SLR on a c-mount would be a reasonable and less expensive way to do photodocumentation.
}
} Thanks
} Paul
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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} 7, 12 -- From: pbarber-at-bu.edu (by way of MicroscopyListserver)
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Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

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From: TindallR-at-missouri.edu
Date: Thu, 25 Aug 2005 15:30:36 -0500
Subject: [Microscopy] Negative processing racks

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

Now that the whole world has gone digital, I just know there must be
bushels of those plastic TEM negative processing racks sitting around
feeling lonely and unwanted. We are starting a special program to
provide a home for these unfortunate victims of technological progress
and would be happy to take a few in.

If you have any of these to spare and would be willing to donate them
for the cost of shipping or a reasonable reimbursement, please let me
know. Thank you for caring.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: hbarwood-at-troy.edu
Date: Thu, 25 Aug 2005 15:46:13 -0500
Subject: [Microscopy] Phosphor Imager help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I recently purchased a used Molecular Dynamics Phosphor Imager. It was, of
course, sold "as is" and I have no idea if I will be able to use it. The
seller stated that it comes on, but the computer shuts down. I have no model
numbers or anything until it actually arrives and I can inspect it. I hope
to use the beast for both X-ray and autoradiography imaging, and perhaps,
X-ray diffraction powder camera work. If anyone on the list uses this brand
of equipment and might be willing to help me diagnose the problems with it,
please reply to me off line. Thanks.

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu


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From: papalia-at-udel.edu
Date: Thu, 25 Aug 2005 17:39:38 -0500
Subject: [Microscopy] viaWWW: TEM cryomicrotiming polymer sample

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (papalia-at-udel.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, August 25, 2005 at 14:12:04
---------------------------------------------------------------------------

Email: papalia-at-udel.edu
Name: John Papalia

Organization: U of Delaware

Title-Subject: [Filtered] TEM cryomicrotiming polymer sample

Question: Good afternoon,

I'm having trouble microtoming some polymer samples and was hoping for any suggestions or guidance that might be out.

My samples are thin films of Polystyrene/Polyethylenepropylene (hydrogenated polyisoprene) evaporatively cast fromm solvent to a thickness of 0.1-0.5 mm.

The problem - I've tried a vast number of different settings, cutting anywhere from -80C down to -140C, and the samples never truly get rigid, although the Tg of the rubber phase falls into the -70 to -90 range based on DSC testing. I basically can not get consistent sections thinner than 100nm, and I need samples at 80nm or thinner.

I have also tried embedding the samples, however this has not helped - the samples themselves stretch and deform even when embedded.

The equipment I have at my disposal is a Leica Ultracut UCT with EMFCS cryo attachment, a Diatome Static Line II, and a Diatome 35 degree cryo/dry diamond knife.

Thanks very much!!!
-John Papalia

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From: walck-at-southbaytech.com
Date: Thu, 25 Aug 2005 19:46:21 -0500
Subject: [Microscopy] Laser pointer images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone know how the images from the toy laser pointers are made?
I've looked at them under a microscope and they look like digitized
images. Are they digital holograms or phase images? I'd like to know
because I'm curious and would like to know how I could make my own
projected image.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


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From: aav_1972-at-yahoo.com
Date: Thu, 25 Aug 2005 22:31:50 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: Microscopy programs in ANY college

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (aav_1972-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, August 25, 2005 at 19:27:55
---------------------------------------------------------------------------

Email: aav_1972-at-yahoo.com
Name: Arnold Villanueva

Education: Undergraduate College

Location: San Diego, California, USA

Question: Hello, I wanted to find out if there were ANY programs in ANY college and/or university that offer either Associate, Bachelors or Masters degrees in Microscopy. What about certificates or professional certifications in the field? Please let me know if you have any information. Thank you and have a nice day :)

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From: Sven.Terclavers-at-med.kuleuven.be
Date: 08/25/05 21:06:52
Subject: [Microscopy] Re: viaWWW: digital SLR to a c-mount on a stereo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul,

I've been using a Nikon D100 onto Zeiss SV11 & Lumar stereomicroscopes
without any problem. No extra lens in the phototube was necessary, the
microscope itself projects the image onto the chip. The adapter was bought
from the company itself and though I've never tried it on a Leica nor
Olympus, I'm quite sure these companies also will be able to provide you the
correct adapters! Indeed a relatove cheap, but good solution for
photodocumentation!
Best regards,

Sven Terclavers

-------Original Message-------

X-from: edelmare-at-muohio.edu

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Paul:

Mounting a digital SLR on a photo-port on a LM is very easy to
do - BUT I do not know of a digital SLR which uses a C-mount
specifically. The c-mounts are very small, digital SLR's, like film
SLR's use larger mounts like a nikon F-mount, Olympus O-mount,
Canon EF-mount, pentax KA-mount, or a more generic T-mount, or
other.

AND you will generally need to use some type of photo-eye
piece lens to project the image to the "film"/"sensor" plane of the
SLR


Your Leica may very well have an adapter on it for the C-
mounting which you will need to replace for a specific SLR body.

On 22 Aug 2005, at 17:56, pbarber-at-bu.edu wrote:

}
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}
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}
} Email: pbarber-at-bu.edu
} Name: Paul Barber
}
} Organization: Boston University
}
} Title-Subject: [Filtered] MListserver:
}
} Question: I would like to be able to attach a digital SLR (ie Nikon D100)
to a c-mount on a stereo microscope like a Leica MZ9.5 or Nikon SMZ800. I've
heard conflicting answers from product reps, some saying that it isn't
possible, some saying it is? However, I have no experience at all
microscopy, but need to buy a system that has at least some
photodocumentation ability. I don't need (and can't afford) a 4-5000 dollar
imaging system. It seems like putting a digital SLR on a c-mount would be a
reasonable and less expensive way to do photodocumentation.
}
} Thanks
} Paul
}
}
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}
} ==============================Original
Headers==============================
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Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
350 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

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From: lethomps-at-us.ibm.com
Date: Fri, 26 Aug 2005 13:38:28 -0500
Subject: [Microscopy] need stain for PS-PEO polymer sample

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Hello-

I have a block copolymer sample (PS-PEO) that I need to cross-section with
the microtome, then stain to localize the PEO. Is there a stain that will
selectively attach to the PEO and not the PS? If so, which staining
method is better, en bloc or on the grid? The sample is in a thin (30-50
nm) layer on a wafer that I remove and embed in epoxy.

Thanks,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099
(408) 927-3856

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From: sstan33-at-yahoo.com
Date: Fri, 26 Aug 2005 14:46:14 -0500
Subject: [Microscopy] Re: need stain for PS-PEO polymer sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Leslie,

Instead of staining PEO segments, you can stain the PS
blocks much easier by using ruthenium acetate.

Wish this helps.

Susheng

--- lethomps-at-us.ibm.com wrote:

}
}
}
}
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}
} Hello-
}
} I have a block copolymer sample (PS-PEO) that I need
} to cross-section with
} the microtome, then stain to localize the PEO. Is
} there a stain that will
} selectively attach to the PEO and not the PS? If
} so, which staining
} method is better, en bloc or on the grid? The
} sample is in a thin (30-50
} nm) layer on a wafer that I remove and embed in
} epoxy.
}
} Thanks,
} Leslie
}
} Leslie Krupp (Thompson)
} IBM Almaden Research
} 650 Harry Road, K19/D2
} San Jose, CA 95120-6099
} (408) 927-3856
}


Susheng TAN, Ph.D
Department of Chemistry, Oklahoma State University
107 Physical Science
Stillwater, Oklahoma 74078 USA
Phone: (405)744-4835 Fax:(405)744-6007
eMail:tsushen-at-okstate.edu sstan33-at-yahoo.com



__________________________________
Yahoo! Mail for Mobile
Take Yahoo! Mail with you! Check email on your mobile phone.
http://mobile.yahoo.com/learn/mail

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From: hanke-at-mee-inc.com
Date: Fri, 26 Aug 2005 16:36:16 -0500
Subject: [Microscopy] Fatigue appearance in C355-T6 aluminum alloy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is a great book on fractography of casting alloys by Gordon
Powell. I believe that the title is "Atlas of Casting Fractures" or
something along those lines. This book has fractographs for various cast
materials produced in the laboratory by various loading methods -
tensile, bend, impact, fatigue, etc. I am not sure if C355 is included,
but similar alloys will be.

The book is out of print, but can often be found at university libraries
or by interlibrary loan from your local public library. I saw a used
copy for sale on Amazon a couple of years ago. (Should have bought it,
but got a copy from the library.)

From my experience, it is not uncommon for fatigue fractures in cast
aluminum to not exhibit fatigue striations. Your description of a
dendritic appearance is a good characterization. Rubbing and smearing
are also not uncommon. Look for recessed areas where the surface is not
rubbed to see the true fracture features.

If the surface is smeared, it is smeared, and the fine fracture features
will be lost. Fatigue striations, especially fine striations from high
cycle fatigue are easily wiped out by smearing from crack closure if the
stress cycle is tension - compression. I don't think that using a higher
KV to penetrate through the smearing will be helpful. In fact, fine
striations are often more easily observed with low KV SE imaging, since
higher KV and BSE do not provide sufficient surface resolution to pick
up these fine features.

Hope this helps.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


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From: jean-ross-at-uiowa.edu
Date: Fri, 26 Aug 2005 17:29:42 -0500
Subject: [Microscopy] viaWWW: Photographic Equip. Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jean-ross-at-uiowa.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, August 26, 2005 at 09:47:21
---------------------------------------------------------------------------

Email: jean-ross-at-uiowa.edu
Name: Jean Ross

Organization: CMRF, University of Iowa

Title-Subject: [Filtered] MListserver:Photographic Equip. Available

Question: Hello everyone,

Our facility is getting out of the photo printing business and we have some equipment that we are willing to give to anyone who wants it and will pay for the shipping to their lab. One is an Ilford Ilfolab MG2650 B+W print processor and the other is a Durst Laborator 1200 point source enlarger. Both are in good working order and assorted spare parts and manuals are included. Email me directly at jean-ross-at-uiowa.edu if you are interested.

Jean Ross
Central Microscopy Research Facility
University of Iowa
85 EMRB
Iowa City, IA 52242
(319)335-8142

---------------------------------------------------------------------------

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From: opto-at-klughammer.de
Date: Fri, 26 Aug 2005 18:09:30 -0500
Subject: [Microscopy] : viaWWW: digital SLR to a cmount on a stereo

Contents Retrieved from Microscopy Listserver Archives
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}
} Hi all,
}
} our company sells adapters which connect all kind of digital consumer cameras, camcorders and SLR cameras to microscope c-mount couplers. The adapters we sell have an extra large lense, so that larger CCDs do not necessarily provide vignetting. We use a basic adapter which includes the magnification lense plus a camera ring which can be chosen individually for the camera.
}
} Everybody who is interested in a list of cameras which can be connected to a microscope may contact us.
}
} with best regards
}
} Anneliese Schmaus
}
} klughammer bio gmbh
} Strassbach 9
} 85229 Markt Indersdorf
}
} Tel. + 49 8136 6011
} Fax +49 8136 7098
}
} schmaus-at-klughammer.de
} www.klughammer.de
}

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From: ramadanhany-at-gmail.com
Date: Fri, 26 Aug 2005 23:04:16 -0500
Subject: [Microscopy] Vacuum technology...........what is the best book?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Guys,
I am new on the list and new to the vacuum technology field. I am looking
for a book that introduces the vacuum technology from experimental prospect
than just the theoretical one, what do you think the best book for this?

Thanks

Hany Ramadan


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4, 23 -- Subject: Vacuum technology...........what is the best book?
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From: cgarber-at-2spi.com
Date: Sat, 27 Aug 2005 04:07:15 -0500
Subject: [Microscopy] Vacuum technology books

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hany Ramadan wrote:
===========================================================
I am new on the list and new to the vacuum technology field. I am looking
for a book that introduces the vacuum technology from experimental prospect
than just the theoretical one, what do you think the best book for this?
===========================================================
In our own laboratory, we have found

Vacuum Methods in Electron Microscopy , Vol. 15 of the series Practical
Methods of Electron Microscopy by Prof. Wilber C. Bigelow


to be very helpful for understanding and solving problems involving vacuum
technology. And apparently so have others as well since it continues to be
a good seller even though it was originally published in 1994. See URL
http://www.2spi.com/catalog/books/book48.shtml This book can be
purchased via numerous other outlets, both on line and in certain university
book stores.

Disclaimer: SPI Supplies is a seller of the above mentioned text so we
might not be seen as an independent party relative to this posting.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================





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From: vray-at-partbeamsystech.com
Date: Sat, 27 Aug 2005 22:40:52 -0500
Subject: [Microscopy] Vacuum technology...........what is the best book?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Hany,

Here are four my favorite vacuum texts:

1. Good introductory book, minimal theory: "A User's Guide to Vacuum
Technology"

http://www.amazon.com/exec/obidos/tg/detail/-/0471270520/qid=1125198579/sr=2
-1/ref=pd_bbs_b_2_1/002-1507630-6605604?v=glance&s=books

2. Advanced and purely practical book on vacuum instrumentation:
"Experimental Innovations in Surface Science: A Guide to Practical
Laboratory Methods and Instruments"

http://www.amazon.com/exec/obidos/tg/detail/-/0387983325/ref=cm_aya_asin.tit
le/002-1507630-6605604?%5Fencoding=UTF8&v=glance

Two good books on vacuum materials, material treatments, and techniques. I
favor the #4, but both are good:

3. "Handbook of Materials and Techniques for Vacuum Devices (AVS Classics in
Vacuum Science and Technology)"

http://www.amazon.com/exec/obidos/tg/detail/-/1563963876/qid=1125199262/sr=1
-4/ref=sr_1_4/002-1507630-6605604?v=glance&s=books

4. "Handbook of Electron Tube and Vacuum Techniques (American Vacuum Society
Classics)"

http://www.amazon.com/exec/obidos/tg/detail/-/1563961210/ref=pd_sim_b_1/002-
1507630-6605604?%5Fencoding=UTF8&v=glance

Enjoy!

Valery Ray
---------------------
Particle Beam Systems
& Technology

-----Original Message-----
X-from: ramadanhany-at-gmail.com [mailto:ramadanhany-at-gmail.com]
Sent: Saturday, August 27, 2005 12:07 AM
To: vray-at-partbeamsystech.com

Hi Guys,
I am new on the list and new to the vacuum technology field. I am looking
for a book that introduces the vacuum technology from experimental prospect
than just the theoretical one, what do you think the best book for this?

Thanks

Hany Ramadan


==============================Original Headers==============================
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From: gary-at-gaugler.com
Date: Sun, 28 Aug 2005 19:41:43 -0500
Subject: [Microscopy] West Nile Virus TEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm looking for TEM or SEM material (specimens
and/or films) of West Nile Virus.

If anyone has these or sub-units, let me know.
I will discuss transfer off-line.

gary g.


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From: olivier.guise-at-ge.com
Date: Sun, 28 Aug 2005 20:39:20 -0500
Subject: [Microscopy] Vacuum technology.... best book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my opinion, the most useful book is: "Experimental innovations in Surface Science" by John T. Yates, Jr.

This book has a strong experimental side to it and presents many examples of vacuum technology with plenty of schematics and illustrations. It comes very handy when one wishes to either design systems or work on a vacuum system for the first time.

Regards,

Olivier


Olivier Guise, Ph.D.

GE Industrial - Plastics
Selkirk Technology Bldg
One Noryl Avenue
Selkirk, NY 12158

P: 518-475-5463
F: 518-475-5856
D: *233-5463
E: olivier.guise-at-ge.com
www.geplastics.com



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From: olivier.guise-at-ge.com
Date: Mon, 29 Aug 2005 08:48:31 -0500
Subject: [Microscopy] SEM - Need Help with Sample Preparation for Cross-section Investigation

Contents Retrieved from Microscopy Listserver Archives
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I am trying to investigate the cross-section of a thick (~1mm) metal substrate coated with a very thin (~1um) silane film. My attempts to prepare a clean cross-section have been unsuccesful and i have not been able to distinguish the thin film from the substrate. So i am wondering if anyone has advice on the best way to prepare a cross-section of this type of sample. Any ideas? Suggestions?


Olivier Guise, Ph.D.

GE Industrial - Plastics
Selkirk Technology Bldg
One Noryl Avenue
Selkirk, NY 12158

P: 518-475-5463
F: 518-475-5856
D: *233-5463
E: olivier.guise-at-ge.com
www.geplastics.com



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From: walck-at-southbaytech.com
Date: Mon, 29 Aug 2005 12:05:50 -0500
Subject: [Microscopy] Vacuum technology...........what is the best book?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've taught a Vacuum Science and Technology course several times. I've
used a couple of books and other references.

The bible, as far as I am concerned, is A. Roth's "Vacuum Technology"
published by North-Holland. I don't know what the latest version is.
This has the all the information both theoretical and practical. The
next best book is John O'Hanlon's "A User's Guide to Vacuum Technology"
published by Wiley. I have the 1st and 2nd edition of this book, but I
don't know whether a newer version is out. I also have Chambers, Fitch,
and Halliday's "Basic Vacuum Technology", but I don't recommend it. One
of the best sources of practical vacuum instruction comes from some
vendors' catalog. I haven't seen one for some time, but the
Leybold-Heraus catalog had a full vacuum science and technolgoy section
in it. Now it is just Leybold Vacuum and you can order the CD with
their product line and a CD with the "Fundamentals of Vacuum Technology"
from them for free after you register.
http://www.leybold.com/index.php?id=257&L=1

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: ramadanhany-at-gmail.com [mailto:ramadanhany-at-gmail.com]
Sent: Friday, August 26, 2005 9:13 PM
To: Walck-at-SouthBayTech.com

Hi Guys,
I am new on the list and new to the vacuum technology field. I am
looking for a book that introduces the vacuum technology from
experimental prospect than just the theoretical one, what do you think
the best book for this?

Thanks

Hany Ramadan


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From: r.sims-at-auckland.ac.nz
Date: Mon, 29 Aug 2005 14:50:34 -0500
Subject: [Microscopy] Vacuum technology reading

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi
I would strongly recommend Wil Bigelow's book.
It's totally relevant to EM work, it's full of both practical
and theoretical stuff, and it's so well-written that it's a
pleasure to read.

cheers

rtch

--
Ritchie Sims Ph D Phone
: 64 9 3737599 ext 87713
Microanalyst Fax :
64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


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From: rbeavers-at-mail.smu.edu
Date: Mon, 29 Aug 2005 15:43:03 -0500
Subject: [Microscopy] Help with Mineral Identification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Group,

Do any of you who run electron microprobe or SEM with EDS analysis, know of any software that can take EDS data calculated as oxide weight percents and generate a chemical formula and a possible mineral ID.

Thanks

Roy Beavers

Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



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From: t.keller-at-uni-jena.de
Date: Tue, 30 Aug 2005 02:32:33 -0500
Subject: [Microscopy] viaWWW: Cryo TEM holder Jeol EM31100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (t.keller-at-uni-jena.de) from
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on
Monday, August 29, 2005 at 04:34:22
---------------------------------------------------------------------------

Email: t.keller-at-uni-jena.de
Name: Thomas Keller

Organization: Friedrich-Schiller-University Jena

Title-Subject: [Filtered] Cryo TEM holder Jeol EM31100

Question: Dear all,

currently I am trying to operate our double-tilt cryo TEM sample
holder Jeol EM31100. Recently there is a Cryo leakage, and also, the
see-saw is loosened from the fixing springs when operated in the TEM.

Is there anybody out there with experience - we want to fix these
issues in our lab. The holder needs to be siginifcantly unmounted and
therefore we are seeking more detailed information.

Best regards,
Thomas

---------------------------------------------------
Dr. Thomas Keller
Institute of Materials Science and Technology (IMT)
Friedrich-Schiller-University Jena
D-07743 Jena

---------------------------------------------------------------------------

==============================Original Headers==============================
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10, 12 -- Subject: viaWWW: Cryo TEM holder Jeol EM31100
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From: nair.ashwin-at-gmail.com
Date: Tue, 30 Aug 2005 02:35:23 -0500
Subject: [Microscopy] viaWWW: Messer Glas Knife cutter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (nair.ashwin-at-gmail.com) from
http://www.microscopy.com/MLFormMail.html on Monday, August 29, 2005
at 17:16:10
---------------------------------------------------------------------------

Email: nair.ashwin-at-gmail.com
Name: Ashwin Nair

Organization: University of Texasat Arlington

Title-Subject: [Filtered] MListserver:

Question: Hi:

I have a Messer Glas Knife cutter in my lab and do not know how to
use it... It would be great if someone could help me find a user
manual for it... I am a student and don't want to mess with the
instrument though I don't mind learning by trial and error...the
instrument is made by Sunkay Laboratory, Tokyo...
Can somebody please help me...

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: donnarl-at-gmail.com
Date: Tue, 30 Aug 2005 02:36:02 -0500
Subject: [Microscopy] viaWWW:CM12 translation control

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (donnarl-at-gmail.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
August 29, 2005 at 17:44:07
---------------------------------------------------------------------------

Email: donnarl-at-gmail.com
Name: Changhui

Title-Subject: [Filtered] translation control

Question: Dear Friends,

The left tranlation control (x) of the stage of our CM12 looks out of
control partially. The left translation control ear (opposite to the
holder loading) can move the stage in/out about 2mm. It can not move
the stage fully. After the certain position, I can still move the
mechnical lever, but the stage does not move. If I hold or press the
holder to the column, I gain the control again.

Does anyone have any idea on how to fix the problem?

Thanks!

Changhui


---------------------------------------------------------------------------

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From: cgarber-at-2spi.com
Date: Tue, 30 Aug 2005 09:12:56 -0500
Subject: [Microscopy] Sunkay Messer Glas Knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ashwin Nair wrote:
==========================================================
I have a Messer Glas Knife cutter in my lab and do not know how to
use it... It would be great if someone could help me find a user
manual for it... I am a student and don't want to mess with the
instrument though I don't mind learning by trial and error...the
instrument is made by Sunkay Laboratory, Tokyo...
Can somebody please help me...
==========================================================
SPI Supplies has been the distributor for Sunkay Laboratories for some
years. We could probably help you if you sent us the serial number on the
back of the unit that you have in your possession. You will find some
information on URL
http://www.2spi.com/catalog/knives/glass.shtml
which might be of further assistance.

Disclaimer: SPI Supplies is a distributor of the Sunkey Laboratories Messer
Glass Knife Maker.

Chuck

PS: Remember that we are 100% paperless and the only way we can keep track
of this kind of correspondence is if you reply using the reply feature of
your e-mail software. That way the entire string of correspondence will be
in one place.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================






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From: wpchan-at-u.washington.edu
Date: Tue, 30 Aug 2005 12:37:44 -0500
Subject: [Microscopy] Re: viaWWW:CM12 translation control

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One possibility. On the CM100, the linkage is fastened wth 2 hex screws
near the x-translation indicator above and below the universal joint. If
they are loose, the stage will fail to move. I think the CM12 may be
similar and it could be a simple fix. Good luck.

--
Pang (Wai Pang Chan, wpchan-at-u.washington.edu)

On Tue, 30 Aug 2005 donnarl-at-gmail.com wrote:

} Email: donnarl-at-gmail.com
} Name: Changhui
}
} Title-Subject: [Filtered] translation control
}
} Question: Dear Friends,
}
} The left tranlation control (x) of the stage of our CM12 looks out of
} control partially. The left translation control ear (opposite to the
} holder loading) can move the stage in/out about 2mm. It can not move
} the stage fully. After the certain position, I can still move the
} mechnical lever, but the stage does not move. If I hold or press the
} holder to the column, I gain the control again.

==============================Original Headers==============================
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From: hinmeigeng-at-hotmail.com
Date: Tue, 30 Aug 2005 14:50:22 -0500
Subject: [Microscopy] PS-PEO staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Leslie,

Here is a response to your question from my friendly local staining expert:

{ { {
My immediate response would be RuO4, although I don't know the relative
reaction rates of the PS and PEO off hand. The basic question here seems to
be is it better to stain and then section or section and then stain?

This depends on how easy the system is to section in the first place. If it
cuts easily, then I'd section and then stain since the problem with the
alternative is controlling the extent of reaction - you get an unstained
interior, a surface layer that completely destroyed and, somewhere in
between, a region that's just right.
} } }

Susheng,

Do you have a reference or two to Ruthenium Acetate staining?

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



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From: sghoshro-at-NMSU.Edu
Date: Tue, 30 Aug 2005 15:54:23 -0500
Subject: [Microscopy] Flatbed scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear everyone,

We are interested in buying a relatively inexpensive ($200-300) flatbed
scanner to scan EM negatives (31/4 x 4") for my EM class. If anyone knows
of a good scanner that comes with medium format film holders and does a
decent scanning job, then please let me know.

Thanks in advance,

Soumitra


*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/

==============================Original Headers==============================
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From: j.compan-at-fz-juelich.de
Date: Tue, 30 Aug 2005 16:44:21 -0500
Subject: [Microscopy] AskAMicroscopist: extinction angle under polarized light

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (j.compan-at-fz-juelich.de) from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, August 30, 2005 at 11:25:21
---------------------------------------------------------------------------

Email: j.compan-at-fz-juelich.de
Name: Compan jeremie

Organization: FZJ

Education: Graduate College

Location: Juelich, Germany

Question: Hi,
I would like to know how to determine a extinction angle under
polarized light!!
I am looking on internet and I do not find any clear answer.
Please can you help me!!

Thanks in advance

Jeremie

---------------------------------------------------------------------------

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From: baskin-at-bio.umass.edu
Date: Tue, 30 Aug 2005 17:12:27 -0500
Subject: [Microscopy] Re: AskAMicroscopist: extinction angle under

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jeremie,
Between crossed polars, when the optical axis of a
birefringent element (a crystal domain) in your sample is parallel to
either the analyzer or the polarizer, that element will not affect
the state of polarization and the field will be dark, as if there
were no sample there at all. So to measure this, you need a rotatable
stage and you need a way to define the axis of your sample to the
axis of the analyzer and polarizer. For example, if you mount an edge
of your sample parallel to the microscope slide. If both polarizer
and analyzer can rotate on you microscope, then you also need to make
sure that you know what direction their transmission axes are
relative to the stage. With these, you can rotate the stage, say in 5
degree increments and look at (record) the intensity of your sample.
When it is maximally dark, the sample is at extinction, and the angle
between the reference direction on your sample and the transmission
axis of the polarizer is the angle of extinction.

Hope this helps,
Tobias
}
} Email: j.compan-at-fz-juelich.de
} Name: Compan jeremie
}
} Organization: FZJ
}
} Education: Graduate College
}
} Location: Juelich, Germany
}
} Question: Hi,
} I would like to know how to determine a extinction angle under
} polarized light!!
} I am looking on internet and I do not find any clear answer.
} Please can you help me!!
}
} Thanks in advance
}
} Jeremie
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
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From: humberto_ibarraa-at-yahoo.com
Date: Tue, 30 Aug 2005 20:52:54 -0500
Subject: [Microscopy] Mitosis video

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to make a video about the process of
mitosis for my school students, what kind of process
would be the most appropiate to use and wich kind of
cells could I use?

Thanks


H.Ibarra
Studium Institute

__________________________________________________
Correo Yahoo!
Espacio para todos tus mensajes, antivirus y antispam ¡gratis!
Regístrate ya - http://correo.espanol.yahoo.com/

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5, 18 -- From: Humberto Ibarra {humberto_ibarraa-at-yahoo.com}
5, 18 -- Subject: Mitosis video
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From: hanke-at-mee-inc.com
Date: Tue, 30 Aug 2005 21:00:08 -0500
Subject: [Microscopy] Re: SEM - Need Help with Sample Preparation for Cross-section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Oliver:

Sample preparation for polymeric films on metallic substrates takes some
practice and patience. If you can prepare good samples of polymer and
metals separately, you should be able to work a procedure to get this
sample adequately prepared.

Coatings at 1um and below are much harder to get consistent reliable
results than for thicker coatings. One possibility to explain your
difficulties is that the coating is thinner than you expect. If you have
good confidence in your sample preparation, you might want to use
another method to check the approximate thickness. We have struggled to
prepare cross sections fo samples only to find that the coating
thickness was really less than 100 angstroms.

If the sample will tolerate compression mounting, you can get good
contrast between the coating and mounting material by wrapping the
sample with aluminum foil. The compression during molding will press the
foil tight against the samples surface and retain the coating during
polishing. Clamping the sample against a flat, conforming backing will
achieve similar but sometimes less satisfactory results if you are using
castable mounting.

Use low nap polishing cloths to avoid rounding during polishing. Beyond
that it may require trial and error of the process to find what works
for this particular substrate and coating material combination.

} I am trying to investigate the cross-section of a thick (~1mm) metal substrate coated with a very thin (~1um) silane film. My attempts to prepare a clean cross-section have been unsuccesful and i have not been able to distinguish the thin film from the substrate. So i am wondering if anyone has advice on the best way to prepare a cross-section of this type of sample. Any ideas? Suggestions?
}
}
} Olivier Guise, Ph.D.
}
} GE Industrial - Plastics
} Selkirk Technology Bldg
} One Noryl Avenue
} Selkirk, NY 12158
}
} P: 518-475-5463
} F: 518-475-5856
} D: *233-5463
} E: olivier.guise-at-ge.com
} www.geplastics.com
}
}
--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


==============================Original Headers==============================
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From: benada-at-biomed.cas.cz
Date: Wed, 31 Aug 2005 02:15:29 -0500
Subject: [Microscopy] Re: viaWWW:CM12 translation control

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
The problem could be in sample holder. There is a slot on the holder that
fits to a guide pin on the goniometer. If there is any dirt, the movement
of holder can be blocked by it. Please, check the slot on the holder for
the dirt. You should also check the width of the slot. The holder has to
move smoothly but without any play on the guide pin.
The precise description of the slot adjustment can be found in the “Single
Tilt Holder Handbook.”
Hopping it helps. Best regards Oldrich
-------------------------------------------
Oldrich Benada
EM lab.
Inst. Microbiology,
Acad. Sci. CR
Czech Republic

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (donnarl-at-gmail.com) from
} http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday,
} August 29, 2005 at 17:44:07
} ---------------------------------------------------------------------------
}
} Email: donnarl-at-gmail.com
} Name: Changhui
}
} Title-Subject: [Filtered] translation control
}
} Question: Dear Friends,
}
} The left tranlation control (x) of the stage of our CM12 looks out of
} control partially. The left translation control ear (opposite to the
} holder loading) can move the stage in/out about 2mm. It can not move
} the stage fully. After the certain position, I can still move the
} mechnical lever, but the stage does not move. If I hold or press the
} holder to the column, I gain the control again.
}
} Does anyone have any idea on how to fix the problem?
}
} Thanks!
}
} Changhui



==============================Original Headers==============================
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4, 25 -- Date: Wed, 31 Aug 2005 09:15:03 +0200 (CEST)
4, 25 -- Subject: Re: [Microscopy] viaWWW:CM12 translation control
4, 25 -- From: =?iso-8859-2?Q?Old=F8ich_Benada?= {benada-at-biomed.cas.cz}
4, 25 -- To: donnarl-at-gmail.com
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From: nmg944-at-uow.edu.au
Date: Wed, 31 Aug 2005 03:18:16 -0500
Subject: [Microscopy] AskAMicroscopist: stained some fixed sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (nmg944-at-uow.edu.au) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Wednesday, August 31, 2005 at 03:16:32
---------------------------------------------------------------------------

Email: nmg944-at-uow.edu.au
Name: Nicole Grant

Organization: University of Wollongong, Australia

Education: Graduate College

Location: Wollongong, NSW, Australia

Question: I have stained some fixed sections (1.25% Glut 4.00%
Paraformaldehyde + PO4 buffer)of lotus (Nulembo nucifera)recepticle
tissue then set in Glycol Methacrylate(GMA) resin. Sectioned slides
were stained with PAS/TBO (periodic acid Schiff's reagent and
Toluidine Blue O). The slides show a number of sections of certain
tissue cells stained green. Do you know what is shown by the green
stain? Green shows up only in epidermal cells on some parts of the
recepticle and it is evenly distributed in other parts. It was
thought that the green could be indicating lipids? Please help. Thanks
Nicole


---------------------------------------------------------------------------

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8, 12 -- From: nmg944-at-uow.edu.au (by way of Ask-A-Microscopist)
8, 12 -- Subject: AskAMicroscopist: stained some fixed sections
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From: t.keller-at-uni-jena.de
Date: Wed, 31 Aug 2005 08:14:42 -0500
Subject: [Microscopy] viaWWW: staining cellulose fibers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form
(NJZFM-ultra-55). It was submitted by
(t.keller-at-uni-jena.de) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html
on Wednesday, August 31, 2005 at 04:33:53
---------------------------------------------------------------------------

Email: t.keller-at-uni-jena.de
Name: Thomas Keller

Organization: Friedrich-Schiller-University Jena

Title-Subject: [Filtered] staining cellulose fibers with silver sulfide

Question: Dear all,


I am looking for a protokol to stain cellulose fibers with silver sulfide.


Any help or link is appreciated.

Thanks Thomas

---------------------------------------------------
Dr. Thomas Keller
Institute of Materials Science and Technology (IMT)
Friedrich-Schiller-University Jena
L–bdergraben 32
D-07743 Jena
Germany
Phone: ++ 49 3641 947742
Fax: ++ 49 3641 947732
Mobile: ++ 49 170 1439522
Internet: t.keller-at-uni-jena.de
---------------------------------------------------
Visit us under http://www.uni-jena.de/matwi/

"Making Materials Science Work 4 U"
---------------------------------------------------


---------------------------------------------------------------------------


==============================Original Headers==============================
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From: microscopytoday-at-tampabay.rr.com
Date: Wed, 31 Aug 2005 10:59:34 -0500
Subject: [Microscopy] Microscopy Today September 2005 Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the September 2005 Microscopy Today table of contents. I will
close the subscription list for this issue on Wednesday September 7, 2005.

Microscopists in North America and MSA members anywhere may have free
subscriptions. Anyone else may subscribe for US$35 per year (to
PARTIALLY cover postage). All subscriptions at
http://www.microscopy-today.com Thank you. Foreign rates will increase
for 2006.

Ron Anderson, Editor
==================================

Getting the Green Light to Measure Small Forces
Stephen W. Carmichael, Mayo Clinic

The Application of Electron Microscopy Techniques to the Space Shuttle
Columbia Accident Investigation
Sandeep Shah and Greg Jerman, Materials Diagnostics Team, NASA Marshall
Space Flight Center

Integrating Light and TEM Information with F-TEM images
P.A. Sims, C.A. Lockwood and J.D. Hardin, Zoology Department, University
of Wisconsin

Fluctuation Microscopy: What is it?
Michael M. J. Treacy, Dept. of Physics and Astronomy, Arizona State
University

TEM of Paraffin-Embedded H&E-Stained Sections for Viral Diagnosis (An
Unusual Papovavirus Case)
Januario C. Estrada, M. Angelica Selim, and Sara E. Miller, Duke
University Medical Center

Energy-Filtered Electron Holography
Rodney A. Herring, University of Victoria, Victoria, BC Canada

Imaging of the Development and Therapeutic Response of an In Vivo Fungal
Catheter Biofilm
Jeniel Nett and David Andes, Department of Medicine, Section of
Infectious Diseases, University of Wisconsin

Eucentric Stage for Recording Stereo Pair Photomacrographs
Ted Clarke, Metallurgical Failure Analysis Consultant

A Novel FIB Method for Preparing Three Dimensional TEM Specimens
Nathan Wang, Cypress Semiconductor Corp., San Jose, CA

ASTM Standards in Microscopy
John J. Friel, Chairman of ASTM Committee E04.11on X-ray and Electron
Metallography

Microscopy in Costa Rica
Alwyn Eades, Lehigh University, Bethlehem, PA

A Stable Lead Citrate Stain for Grids
Leona Cohen-Gould, Weill Medical College of Cornell University

New and Interesting at M&M-2005

Industry News

NetNotes


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From: eoptics-at-mcmaster.ca
Date: Wed, 31 Aug 2005 17:30:39 -0500
Subject: [Microscopy] viaWWW: Wanted 50 piece TEM Film rack

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (eoptics-at-mcmaster.ca) from
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on
Wednesday, August 31, 2005 at 09:23:51
---------------------------------------------------------------------------

Email: eoptics-at-mcmaster.ca
Name: Fred Pearson

Organization: McMaster University

Title-Subject: [Filtered] MListserver:Wanted 50 piece TEM Film rack

Question: Good day:

I am looking for a film developing rack that was distributed by J.B
EM. Services Inc. back in the late 80's. It holds 50 3 1/4" x 4" e.
m. film. This type is a bit different from the those available in EM
supply catalogues today. At the top where the film slides into the
slot, there is a slight curvature or depression which allows for easy
insertion of the film when you are in the dark. What I see in
catalogues now, is that the slot insertion for the same type of rack
has only a straight narrow slot which is very difficult to use.

Also, I can't find any record now of this company J.B. EM Services in
any part of Quebec.....did they disslove?

thanks in advance

Fred

---------------------------------------------------------------------------

==============================Original Headers==============================
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10, 12 -- From: eoptics-at-mcmaster.ca (by way of MicroscopyListserver)
10, 12 -- Subject: viaWWW: Wanted 50 piece TEM Film rack
10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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From: ktrou-at-nb.utmem.edu
Date: Wed, 31 Aug 2005 17:31:11 -0500
Subject: [Microscopy] viaWWW: parts needed 2168 LKB Ultrostainer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ktrou-at-nb.utmem.edu) from
http://www.microscopy.com/MLFormMail.html on Wednesday, August 31,
2005 at 13:35:04
---------------------------------------------------------------------------

Email: ktrou-at-nb.utmem.edu
Name: Kathy Troughton

Organization: University of Tennessee, Memphis

Title-Subject: [Filtered] Spare parts for LKB 2168 Ultrostainer

Question: Does anyone know where I can procure spare parts for the
2168 LKB Ultrostainer, specifically, the valves involved in
exchanging solutions?

Kathy Troughton
University of Tennessee, Memphis
855 Monroe, 311 Link Building
Memphis, TN 38163
901-448-5976
ktrou-at-nb.utmem.edu

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: joanne.alewine-at-med.va.gov
Date: Wed, 31 Aug 2005 17:31:48 -0500
Subject: [Microscopy] AskAMicroscopist: Zeiss EM900 Electron Microsc0pe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (joanne.alewine-at-med.va.gov) from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Wednesday, August 31, 2005 at 09:41:46
---------------------------------------------------------------------------

Email: joanne.alewine-at-med.va.gov
Name: Joanne Alewine

Organization: VA Ann Arbor Healthcare System

Education: 9-12th Grade High School

Location: City, State, Country

Question: I am looking for businesses that are capable of doing
Preventative Maintenance and Repairs for:

Zeiss EM900 Electron Microsc0pe

Your help would be appreciated

---------------------------------------------------------------------------

==============================Original Headers==============================
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9, 12 -- From: joanne.alewine-at-med.va.gov (by way of Ask-A-Microscopist)
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From: sheris-at-MIT.EDU
Date: Thu, 1 Sep 2005 08:54:00 -0500
Subject: [Microscopy] LM- permeability of epoxy sections to probes?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm hoping someone on the list has some expertise that they could share
with me. I've searched the list archives but didn't turn up anything. I'm
working with some thick sections (about 1 mm) embedded in Spurr's low
viscosity epoxy resin. The goal is to incubate these sections with
fluorescently labelled nucleic acid probes. The first thing I plan to do
is try incubating these with a Polysciences epoxy removal kit to make the
cells accessible for staining. I'm wondering if anyone has experience with
using nucleic acid probes on thin sections, or could direct me to some
good references. Is there anything I could use to increase the
permeability of the epoxy, or are my probes just going to bind to whatever
is exposed with the epoxy removal solution?
Also, could anyone recommend a way to fix the sections on coverslips for
manipulation in the staining procedure and later viewing with
epifluorescence?

Thanks.


---------------------------------------------------
Sheri Simmons
Geomicrobiology Group
MIT-WHOI Joint Program, Biological Oceanography
http://www.whoi.edu/science/MCG/edwards/sherisimmons

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From: TindallR-at-missouri.edu
Date: Fri, 2 Sep 2005 09:22:08 -0500
Subject: [Microscopy] Cryo SEM: Freeze drying question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Here's one for the "never a dull moment" category. I have occasionally
used the freeze-drying effect of our FESEM's cryostage to sublime ice
off of LN2 plunge-frozen samples. Now I have someone who would like to
try this with liquids such as benzene and other solvents. I'm willing
to give it a shot, but have no clue how such liquids behave at low
temperatures. Our client says the solvents will freeze at the
temperatures we operate our stage at, but she also has no idea whether
"freeze drying" will occur with them.

Has anyone tried this? As usual, I'll be checking the literature, but
personal experiences are always the most useful, and I figure you folks
have collectively done it all at some point.

Thanks for all the help----past, present, and future.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: neuberger1234-at-comcast.net
Date: Fri, 2 Sep 2005 10:16:25 -0500
Subject: [Microscopy] Cryo SEM: Freeze drying question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

I assume that in checking the literature you will be determining the
temperature and pressure at which each organic will sublime and the
possibility of contaminating the chamber and other lower parts of the
column. I assume that you are using an anticontaminator around/above the
specimen holder; have you every lost control of the temperature or otherwise
have the specimen "warm" up to room temp under the beam? Working with
frozen organic solvents is something that I would contact your SEM
manufacturer about and get their input.

I also wonder why you are getting ice on your samples. Are you using a
device and method of avoiding ice contamination? Do you use LN2 slush?
Having worked also with liquid helium 4 to freeze biological specimens, ice
contamination was not an option so it can be done.

Damian Neuberger, Ph.D.
Consultant
Microscopy/Digital Imaging/Image Analysis
2416 Covert Rd
Glenview IL 60025
Tel: (847) 998-8574
email: neuberger1234-at-comcast.net {mailto:neuberger1234-at-comcast.net}


-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Friday, September 02, 2005 9:22 AM
To: neuberger1234-at-comcast.net

Dear Listers,

Here's one for the "never a dull moment" category. I have occasionally
used the freeze-drying effect of our FESEM's cryostage to sublime ice
off of LN2 plunge-frozen samples. Now I have someone who would like to
try this with liquids such as benzene and other solvents. I'm willing
to give it a shot, but have no clue how such liquids behave at low
temperatures. Our client says the solvents will freeze at the
temperatures we operate our stage at, but she also has no idea whether
"freeze drying" will occur with them.

Has anyone tried this? As usual, I'll be checking the literature, but
personal experiences are always the most useful, and I figure you folks
have collectively done it all at some point.

Thanks for all the help----past, present, and future.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu



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From: TindallR-at-missouri.edu
Date: Fri, 2 Sep 2005 10:17:45 -0500
Subject: [Microscopy] Cryo SEM: Freeze drying question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A follow-up to my first question: I've just been reminded of the
obvious hazard of solvent fumes, although the amounts involved would be
quite small. Does anyone know if the mist traps on rotary pumps would
be effective at trapping these? Our RPs are, unfortunately, in the same
room as the scope.

Thanks,
Randy

-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Friday, September 02, 2005 9:25 AM
To: Tindall, Randy D.

Dear Listers,

Here's one for the "never a dull moment" category. I have occasionally
used the freeze-drying effect of our FESEM's cryostage to sublime ice
off of LN2 plunge-frozen samples. Now I have someone who would like to
try this with liquids such as benzene and other solvents. I'm willing
to give it a shot, but have no clue how such liquids behave at low
temperatures. Our client says the solvents will freeze at the
temperatures we operate our stage at, but she also has no idea whether
"freeze drying" will occur with them.

Has anyone tried this? As usual, I'll be checking the literature, but
personal experiences are always the most useful, and I figure you folks
have collectively done it all at some point.

Thanks for all the help----past, present, and future.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: mmckilli-at-smurfit.com
Date: Fri, 2 Sep 2005 11:26:51 -0500
Subject: [Microscopy] viaWWW: Image analysis software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mmckilli-at-smurfit.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
September 2, 2005 at 08:32:34
---------------------------------------------------------------------------

Email: mmckilli-at-smurfit.com
Name: M McKillip

Title-Subject: [Filtered] Image analysis software...

Question: We recently purchased a Nikon digital camera for optical
microscopes as well as Image Pro image analysis software.

We have abandoned use of Image Pro software because of contant
problems. Does anyone have recommendations for comparable image
analysis software packages?

---------------------------------------------------------------------------

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From: DrJohnRuss-at-aol.com
Date: Fri, 2 Sep 2005 11:41:05 -0500
Subject: [Microscopy] Re: viaWWW: Image analysis software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 9/2/05 12:27:37 PM, mmckilli-at-smurfit.com writes:

} We have abandoned use of Image Pro software because of contant
} problems. Does anyone have recommendations for comparable image
} analysis software packages?

What kind of problems? Is there some kind of processing or measurement you
need that is not provided, or is the software crashing, or do you just not
understand how to use it? Image Pro Plus is a widely used and generally rather
powerful package. Certainly there are others, including ones that are
"comparable." But they may present "comparable" problems for your application, too.


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From: Sue.Tyler-at-noaa.gov
Date: Fri, 2 Sep 2005 12:43:09 -0500
Subject: [Microscopy] TEM best fixative question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Could you advise me on the best primary fixative to use for marine
species that need both light microscopy and TEM? Currently we are
fixing our specimens in 2.5%Glut/Form in cacodylate buffer and
bisecting the animal -half for em and half for light microscopy. We
perform histology on the LM half first, then if we need to retrieve the
em fixed tissue we have the ability. In an effort to reduce work load
and tissue storage do you have other suggestions?

Sue Tyler
Dept. of Commerce
Cooperative Oxford Laboratory
Oxford, MD.
sue.tyler-at-noaa.gov

==============================Original Headers==============================
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From: sszewczyk-at-arl.army.mil
Date: Fri, 2 Sep 2005 12:55:33 -0500
Subject: [Microscopy] Cryo SEM: Freeze drying question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,
Have a look at http://www.sisweb.com/referenc/applnote/app-84.htm

I found this application note related to your follow-up question while
looking for a new mist filter for our mechanical pump. It seems that
normal oil mist filters are only effective at trapping the high
molecular weight hydrocarbons from pump oil.

Steve Szewczyk
ORISE Contractor, US Army Research Lab



-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Friday, September 02, 2005 11:20 AM
To: Szewczyk, Steven (Cont, ARL/WMRD)

A follow-up to my first question: I've just been reminded of the
obvious hazard of solvent fumes, although the amounts involved would be
quite small. Does anyone know if the mist traps on rotary pumps would
be effective at trapping these? Our RPs are, unfortunately, in the same
room as the scope.

Thanks,
Randy

-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Friday, September 02, 2005 9:25 AM
To: Tindall, Randy D.

Dear Listers,

Here's one for the "never a dull moment" category. I have occasionally
used the freeze-drying effect of our FESEM's cryostage to sublime ice
off of LN2 plunge-frozen samples. Now I have someone who would like to
try this with liquids such as benzene and other solvents. I'm willing
to give it a shot, but have no clue how such liquids behave at low
temperatures. Our client says the solvents will freeze at the
temperatures we operate our stage at, but she also has no idea whether
"freeze drying" will occur with them.

Has anyone tried this? As usual, I'll be checking the literature, but
personal experiences are always the most useful, and I figure you folks
have collectively done it all at some point.

Thanks for all the help----past, present, and future.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: David_Bell-at-millipore.com
Date: Fri, 2 Sep 2005 13:25:02 -0500
Subject: [Microscopy] Re: viaWWW: Image analysis software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What type of problems are you experiencing with Image Pro? We've been
using IPP for the past eight years without much incident. Is it technical
problems with the hardware/software, or is it a matter of training?
Perhaps you're not aware of the new forum they provide to discuss issues
and applications of their software. The URL for the forum is:

http://www.mediacy.com/ipp/ippforum/

This is an excellent source of information about the software that you can
utilize before just tossing the several thousand dollars spent on it! Feel
free to ask me off line and I'll see if I can help you also.

Regards,



David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108





mmckilli-at-smurfit.com
09/02/2005 12:27 PM
Please respond to
mmckilli-at-smurfit.com


To
David_Bell-at-Millipore.com
cc

Subject
[Microscopy] viaWWW: Image analysis software









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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mmckilli-at-smurfit.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
September 2, 2005 at 08:32:34
---------------------------------------------------------------------------

Email: mmckilli-at-smurfit.com
Name: M McKillip

Title-Subject: [Filtered] Image analysis software...

Question: We recently purchased a Nikon digital camera for optical
microscopes as well as Image Pro image analysis software.

We have abandoned use of Image Pro software because of contant
problems. Does anyone have recommendations for comparable image
analysis software packages?

---------------------------------------------------------------------------

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From: mcauliff-at-umdnj.edu
Date: Fri, 2 Sep 2005 14:03:06 -0500
Subject: [Microscopy] Re: TEM best fixative question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Sue:

Your question is very broad. Are you unhappy with the results you
are getting? If so, exactly what is wrong? And which "marine species"
are you working with? Have you checked the literature on the species of
interest to see what others are using and what kind of results they are
getting? I think you will have to provide more information if you want a
specific recommnedation.

Geoff

Sue.Tyler-at-noaa.gov wrote:

} Could you advise me on the best primary fixative to use for marine
} species that need both light microscopy and TEM? Currently we are
} fixing our specimens in 2.5%Glut/Form in cacodylate buffer and
} bisecting the animal -half for em and half for light microscopy. We
} perform histology on the LM half first, then if we need to retrieve the
} em fixed tissue we have the ability. In an effort to reduce work load
} and tissue storage do you have other suggestions?
}
} Sue Tyler
} Dept. of Commerce
} Cooperative Oxford Laboratory
} Oxford, MD.
} sue.tyler-at-noaa.gov
}
}
}
}


--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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8, 31 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu}
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From: mark-at-brickley.plus.com
Date: Sat, 3 Sep 2005 15:19:49 -0500
Subject: [Microscopy] optronics microfire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

We are considering buying a digital camera for imaging a wide range of
subjects (isn't everyone!) though our first project is centered on
chlamydomonas fluorescence work particularly chloroplast
auto-fluorescence.

So far the optronics microfire looks like a good camera that might suit
our needs and I would very much appreciate any comments on this camera
from anyone who has had experience with one.

Many thanks in advance

Mark Brickley


==============================Original Headers==============================
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From: pjm-at-gol.com
Date: Mon, 5 Sep 2005 04:02:08 -0500
Subject: [Microscopy] relocating objects on a flipped slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,

With LM, I would like to look at starch granules from the topside and
bottomside of a slide, in order to get more information about shape.

I can do this by flipping the slide over, and raising it slightly off
the platten so that the coverslip is not scraped off - but to
relocate the same object on the slide might require a lot of
searching.

Can anyone suggest a simple mathematical formula that can be used to
calculate new co-ordinates from (a) the linear dimensions of the
slide (L x W), and (b) the mm grid co-ordinates of the object on the
slide?

Does a formula/conversion-table already exist for this?

Yours hopefully, Peter
--
Peter Matthews (Dr)
National Museum of Ethnology
Senri Expo Park, Suita City
Osaka 565-8511, Japan
Tel. +81 6 6876-2151 (museum)
Tel. +81 6 6876-8357 (Peter's office)
Fax +81 6 6878-7503 (museum)

Websites:

The Research Cooperative http://www.researchco-op.co.nz

A meeting place for research writers, editors, translators and proofreaders


2003 Conference on Research Writing in Japan:

http://www.researchco-op.net/conference.html


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From: bjg-at-cmm.uwa.edu.au
Date: Mon, 5 Sep 2005 06:27:21 -0500
Subject: [Microscopy] viaWWW: open positions at UWA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In principle it is real simple
x(new) = Length of slide - x(original)
y should remain the same if the slide is perfectly rectangular and the
geometry of the stage is perfect.
You will need to correct for any discrepancy between zero on the stage scale
and the end of the slide (x=0).

Chris

----- Original Message -----
X-from: {pjm-at-gol.com}
To: {cjeffree-at-staffmail.ed.ac.uk}
Sent: Monday, September 05, 2005 10:02 AM

Below is the result of your feedback form
(NJZFM-ultra-55). It was submitted by
(bjg-at-cmm.uwa.edu.au) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html
on Monday, September 5, 2005 at 04:08:22
---------------------------------------------------------------------------

Email: bjg-at-cmm.uwa.edu.au
Name: Brendan J Griffin

Organization: The University of Western Australia

Title-Subject: [Filtered] MListserver:open positions

Question: Dear all

The following two positions are available in my
lab. Both are ongoing, ie permanent, in a
fantastic place, and a dollar here is about a
dollar anywhere. Happy to answer any queries.

Cheers

Brendan

SENIOR TECHNICIAN (REF: 980)
CENTRE FOR MICROSCOPY AND MICROANALYSIS

o Ongoing appointment
o Salary range: HEE Level 5 $44,204 - $49,149 p.a.
o Closing date: Tuesday, 20 September 2005

The Centre for Microscopy and
Microanalysis (CMM) supports application of
light, electron and laser microscopy techniques
to a wide range of research, see http:
//cmm.uwa.edu.au, as part of major regional and
national collaborations.

We are seeking a person who is:
o Client and quality focused
o Keen to embrace new responsibilities and develop new skills

Application Details: Interested
applicants must obtain the application package
and address the prerequisites and selection
criteria. These essential details can be
accessed from the vacancy page on http:
//jobs.uwa.edu.au/ or the 24 hour ìhotlineî on
6488 3733. To discuss or clarify any aspects of
the position please contact the Director of the
CMM, Associate Professor Brendan Griffin on 6488
2770 or email admin-at-cmm.uwa.edu.au.

National Employer of Choice for Women

**********

ASSOCIATE LECTURER/LECTURER (REF: 983)
CENTRE FOR MICROSCOPY AND MICROANALYSIS

o Tenurable appointment
o Salary Range: Associate Lecturer Level A
$42,843 - $58,140 p.a. (Minimum starting salary
for appointee with PhD will be $54,163 p.a.)
o Salary range: Lecturer Level B $61,201 - $72,678 p.a.
o Closing date: Friday, 7 October 2005

A CAMECA nanoSIMS 50 high-resolution ion
microprobe was installed in the Centre for
Microscopy and Microanalysis (CMM) at The
University of Western Australia, as part of the
NANO-MNRF, in June, 2003. The NANO-MNRF
(www.nano.org.au) is a Major National Research
Facility that links advanced nano-scale
characterisation equipment at the Universities of
Sydney, New South Wales, Queensland, Western
Australia (UWA) and Melbourne. The range of CMM
microscopy instrumentation and expertise is
extensive (see http: //cmm.uwa.edu.au) such that
the centre and MNRF represent a premium
environment for research services, research
training and research programs. UWA is also a
major partner in local research consortia in
isotope science with a range of stable and
radiogenic isotope facilities, including two
SHRIMP-II. We are seeking a highly motivated,
self-guided scientist who has the ability to work
with other staff within the Centre and its users.

The prime responsibility will be to manage a
CAMECA nanoSIMS 50 ion microprobe as a
world-class National Facility. The position will
have the core role in a strong team led locally
by Associate Professor Brendan Griffin and
nationally by Professor Simon Ringer (NANO MNRF
Executive Director). The level of appointment
will be based on qualifications and experience.
For appointment at Level A applicants must have a
relevant degree preferably with a PhD and for
appointment at Level B applicants must have a
relevant degree with a PhD. Applicants with
teaching experience are requested to submit a
teaching portfolio as part of their application.
For further information regarding the position
please contact the Director of the CMM, Associate
Professor Brendan Griffin on (08) 6488 2770 or
email bjg-at-cmm.uwa.edu.au.

Located adjacent to the picturesque banks of the
Swan River, the University offers an attractive
benefits package including generous
superannuation and leave provisions, fares to
Perth (if applicable) for appointee and
dependants along with a removals allowance and an
enviable working environment. These and other
benefits will be specified in the offer of
employment.

APPLICATION DETAILS: For copies of the
position description please access the website
http: //jobs.uwa.edu.au/. Applicants must address
the prerequisites and selection criteria. Written
applications quoting the reference number,
personal contact details, qualifications and
experience, along with contact details of three
referees should be sent to Director, Human
Resources, The University of Western Australia,
M350, 35 Stirling Highway, Crawley WA 6009 or
emailed to jobs-at-uwa.edu.au by the closing date.

National Employer of Choice for Women


---------------------------------------------------------------------------


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From: sheris-at-MIT.EDU
Date: Mon, 5 Sep 2005 19:01:58 -0500
Subject: [Microscopy] LM- DAPI staining of cell inclusions?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
Does anyone know if DAPI can nonspecifically bind to cellular inclusions
(i.e. elemental sulfur or PHB) in addition to DNA?

Thanks,
Sheri


---------------------------------------------------
Sheri Simmons
Geomicrobiology Group
MIT-WHOI Joint Program, Biological Oceanography
http://www.whoi.edu/science/MCG/edwards/sherisimmons

==============================Original Headers==============================
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From: hinmeigeng-at-hotmail.com
Date: Tue, 6 Sep 2005 02:47:20 -0500
Subject: [Microscopy] Re: Cryo SEM: Freeze drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

In our group (polymer physics) a long time ago, freeze drying of para-xylene
(m.p. 19°C, b.p. ~ 144°C) was used. There are quite a few organic solvents
freezing around 0°C, and many of them should respond to this technique.
What is important is the vapour pressure, so things with too high a b.p.
wouldn't work.

However, as the other replies state, it's the pumping system one should
worry about.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



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From: gwe-at-ufl.edu
Date: Tue, 6 Sep 2005 09:41:49 -0500
Subject: [Microscopy] Replies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone else miss seeing the answers to questions. Now that replies
do not go to the list we are missing out on a lot. Folks need to
remember to add the list to their replies, unless the reply is intended
only for the original sender

--
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
Phone: 352-392-1295
Fax: 352 846 0251



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From: NRANIERI-at-ORA.FDA.GOV
Date: Tue, 6 Sep 2005 10:25:46 -0500
Subject: [Microscopy] viaWWW: Image analysis software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

IPP is a powerful application and of course depending on the version you are
using, it may have some limitations (by features that you may not able to
program for yourself). However IPP is as powerful as the user can use it.
There are other applications out there and one application that is not
driven by programming ability is MetaMorph by a company that used to be
called Universal Imaging, recently it has been under a company called
Molecular Devices. Check it out but don't give up on IPP so easily.

Ciao!
Nico.

-----Original Message-----
X-from: mmckilli-at-smurfit.com [mailto:mmckilli-at-smurfit.com]
Sent: Friday, September 02, 2005 12:29 PM
To: Ranieri, Nicola

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (mmckilli-at-smurfit.com) from
http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
September 2, 2005 at 08:32:34
---------------------------------------------------------------------------

Email: mmckilli-at-smurfit.com
Name: M McKillip

Title-Subject: [Filtered] Image analysis software...

Question: We recently purchased a Nikon digital camera for optical
microscopes as well as Image Pro image analysis software.

We have abandoned use of Image Pro software because of contant
problems. Does anyone have recommendations for comparable image
analysis software packages?

---------------------------------------------------------------------------

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From: sghoshro-at-NMSU.Edu
Date: Tue, 6 Sep 2005 10:29:59 -0500
Subject: [Microscopy] Thank you for scanner info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to everyone who replied to my question about purchasing flatbed
scanner to scan TEM negatives. Most of you suggested EPSON and
Canon scanners. So we will go with one with a reasonable price tag.

Best wishes,

Soumitra

*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/

==============================Original Headers==============================
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From: Sven.Terclavers-at-med.kuleuven.be
Date: Tue, 6 Sep 2005 10:39:52 -0500
Subject: [Microscopy] Re: Image analysis software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would consider the Zeiss AxioVision 4.0 software. Accurate, very
user-friendly and with quite a lot of basic functions that can be even
more expanded. Wizards and record-functions help you to automate
repetitive analysis... I have no personal benefit if you would start
using this software, don't get me wrong, it just 'dazzled' me with a
few things in such a way that I even bought the VBA-module to start
programming in Visual Basic to even more expand it's possibilities!
Besides this, a very helpful forum has been created to exchange
questions, answers, programs etc., just as this forum.
Best,

Sven Terclavers



Quoting NRANIERI-at-ORA.FDA.GOV:

}
}
}
} ----------------------------------------------------------------------
------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
------
}
} IPP is a powerful application and of course depending on the version
} you are
} using, it may have some limitations (by features that you may not
} able to
} program for yourself). However IPP is as powerful as the user can use
} it.
} There are other applications out there and one application that is
} not
} driven by programming ability is MetaMorph by a company that used to
} be
} called Universal Imaging, recently it has been under a company
} called
} Molecular Devices. Check it out but don't give up on IPP so easily.
}
} Ciao!
} Nico.
}
} -----Original Message-----
} X-from: mmckilli-at-smurfit.com [mailto:mmckilli-at-smurfit.com]
} Sent: Friday, September 02, 2005 12:29 PM
} To: Ranieri, Nicola
} Subject: [Microscopy] viaWWW: Image analysis software
}
}
}
}
} ----------------------------------------------------------------------
------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (mmckilli-at-smurfit.com) from
} http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday,
}
} September 2, 2005 at 08:32:34
} ----------------------------------------------------------------------
-----
}
} Email: mmckilli-at-smurfit.com
} Name: M McKillip
}
} Title-Subject: [Filtered] Image analysis software...
}
} Question: We recently purchased a Nikon digital camera for optical
} microscopes as well as Image Pro image analysis software.
}
} We have abandoned use of Image Pro software because of contant
} problems. Does anyone have recommendations for comparable image
} analysis software packages?
}
} ----------------------------------------------------------------------
-----
}
} ==============================Original
} Headers==============================
} 6, 12 -- From zaluzec-at-microscopy.com Fri Sep 2 11:26:50 2005
} 6, 12 -- Received: from [192.168.2.125] (msdvpn22.msd.anl.gov
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} ==============================Original
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} -0500
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} 13, 16 -- From: "Ranieri, Nicola" {NRANIERI-at-ORA.FDA.GOV}
} 13, 16 -- To: "'Microscopy-at-microscopy.com'"
} {Microscopy-at-microscopy.com}
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}




Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm


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From: cbalane-at-wesleyan.edu
Date: Tue, 6 Sep 2005 12:14:00 -0500
Subject: [Microscopy] Re: LM- DAPI staining of cell inclusions?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,
In my experience, DAPI very specifically stains DNA nicely. I have also
used other DNA stains like Sytox but I get better definition in
DNA/nuclear labeling with DAPI when taking epifluorescent micrographs.

For what concentration of DAPI to use in your specimen, I would recommend
a PubMed search.

I hope this helps.

With much sincerity,
Carlo



Carlo Franco Bolivar Balane

Box 4058, 222 Church Street, or Wolfe Laboratory
Wesleyan University Station Rm. 157, HA Laboratories
Middletown, CT, 06459-4058 Wesleyan University
phone: 1.860.759.2830 phone: 1.860.685.3275


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From: zaluzec-at-microscopy.com
Date: Wed, 7 Sep 2005 03:20:54 -0500
Subject: [Microscopy] Administrivia: Replies

Contents Retrieved from Microscopy Listserver Archives
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Greg etal....

I concur with you Greg, I personally prefer the old method.
However, the change was made about a month ago
as we were getting far too many inadvertent postings
and the rash that occurred at the end of July was getting just
too much, particularly with the almost immediate
followup of an apology.

For the time being, just a reminder to all subscribers.
Please remember (and this is documented in the Instructions
if you are replying to a posting and consider the information of
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It is also appropriate should an individual collects a series of answers
/solutions to their question that (s)he compose a summary of
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included in the
archives and provide a convenient mechanism for others to
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engine

Cheers

Nestor
Your Friendly Neighborhood SysOp





At 9:41 AM -0500 9/6/05, gwe-at-ufl.edu wrote:
} ----------------------------------------------------------------------------
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From: ishask2aol.com-at-ns.microscopy.com
Date: Wed, 7 Sep 2005 03:27:56 -0500
Subject: [Microscopy] viaWWW: fixing and viewing plant on tem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (ishask2aol.com) from
http://microscopy.com/MLFormMail.html on Tuesday, September 6, 2005
at 00:24:01
---------------------------------------------------------------------------

Email: ishask2aol.com
Name: isha

Organization: san joaquin delta college, electron microscopy program

Title-Subject: [Filtered] fixing and viewing plant on tem

Question: i am a EM student at san joaquin delta college in CA. we
have a project coming up where we have to fix, section, stain and
view plant tissue on the tem.
so i was wondering which part of a plant will be interesting to look
at...or is there a particular plant that i should look for
thanks
isha



---------------------------------------------------------------------------

==============================Original Headers==============================
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From: rberry-at-rsbs.anu.edu.au
Date: Wed, 7 Sep 2005 03:28:23 -0500
Subject: [Microscopy] viaWWW: en-bloc stains for light microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (rberry-at-rsbs.anu.edu.au) from
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
Tuesday, September 6, 2005 at 20:57:06
---------------------------------------------------------------------------

Email: rberry-at-rsbs.anu.edu.au
Name: Richard Berry

Organization: CVS, RSBS, ANU

Title-Subject: [Filtered] en-bloc stains for light microscopy

Question: Hello there,

I was just wondering if anyone had ever happened upon a reliable
method for en-bloc staining insect neural tissue in araldite embedded
specimens.

I have tried a few things so far, including, toluidine blue,
ferricyanide and p-phenylaminediamine. While these seem to help none
of them give satisfactory contrast without post-staining the sections
with tol. blue.

Does anyone know of something that will stain membrane structure well
and can be used en-bloc? The specimens do not have to be embedded in
araldite embedded...just something that will cut 1-2 um sections.

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: baskin-at-bio.umass.edu
Date: Wed, 7 Sep 2005 08:38:52 -0500
Subject: [Microscopy] Re: viaWWW: fixing and viewing plant on tem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Isha,
Well, as a plant biologist, I have to say that *all* parts of
the plant are interesting! But perhaps it is worth mentioning that
root tips may be easier to fix and prep than other parts because they
lack cuticles, air spaces, and wood, all of which can give rise to
sample prep issues. Of course, all of these other parts can be preped
too so follow your own curiosity.

Tobias


}
} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (ishask2aol.com) from
} http://microscopy.com/MLFormMail.html on Tuesday, September 6, 2005
} at 00:24:01
} ---------------------------------------------------------------------------
}
} Email: ishask2aol.com
} Name: isha
}
} Organization: san joaquin delta college, electron microscopy program
}
} Title-Subject: [Filtered] fixing and viewing plant on tem
}
} Question: i am a EM student at san joaquin delta college in CA. we
} have a project coming up where we have to fix, section, stain and
} view plant tissue on the tem.
} so i was wondering which part of a plant will be interesting to look
} at...or is there a particular plant that i should look for
} thanks
} isha
}
}
} ---------------------------------------------------------------------------
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
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5, 17 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
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From: mcauliff-at-umdnj.edu
Date: Wed, 7 Sep 2005 09:20:39 -0500
Subject: [Microscopy] Re: viaWWW: en-bloc stains for light microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Richard:

The usual en bloc stains used for TEM work probably won't give you
enough contrast for LM unless you try phase contrast or Nomarski
illumination.
p-phenylenediamine will reduce the osmium in the tissue and increase
contrast but is not a stain in the usual sense.
If you used ferricyanide-osmium and en bloc staining with uranyl acetate
you might get enough contrast for your needs. Only you can make that
judgement.
Toluidine blue is leached out of tissue very rapidly in the ascending
ethanols used for dehydration. Try Cresyl Violet or Thionin instead OR
dehydrate in acetone instead of alcohols. I don't think either will show
up (much) in the EM though.

Geoff


rberry-at-rsbs.anu.edu.au wrote:

} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (rberry-at-rsbs.anu.edu.au) from
} http://www.microscopy.com/MicroscopyListserver/MLFormMail.html on
} Tuesday, September 6, 2005 at 20:57:06
} ---------------------------------------------------------------------------
}
} Email: rberry-at-rsbs.anu.edu.au
} Name: Richard Berry
}
} Organization: CVS, RSBS, ANU
}
} Title-Subject: [Filtered] en-bloc stains for light microscopy
}
} Question: Hello there,
}
} I was just wondering if anyone had ever happened upon a reliable
} method for en-bloc staining insect neural tissue in araldite embedded
} specimens.
}
} I have tried a few things so far, including, toluidine blue,
} ferricyanide and p-phenylaminediamine. While these seem to help none
} of them give satisfactory contrast without post-staining the sections
} with tol. blue.
}
} Does anyone know of something that will stain membrane structure well
} and can be used en-bloc? The specimens do not have to be embedded in
} araldite embedded...just something that will cut 1-2 um sections.
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 9, 12 -- From zaluzec-at-microscopy.com Wed Sep 7 03:28:23 2005
} 9, 12 -- Received: from [131.111.102.55] (msdvpn24.msd.anl.gov [130.202.238.88])
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} 9, 12 -- From: rberry-at-rsbs.anu.edu.au (by way of MicroscopyListserver)
} 9, 12 -- Subject: viaWWW: en-bloc stains for light microscopy
} 9, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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}
}
}


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: dsherman-at-purdue.edu
Date: Wed, 7 Sep 2005 10:41:04 -0500
Subject: [Microscopy] Re: viaWWW: fixing and viewing plant on tem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I understand that a true plant biologist such as Tobias would find all plant
tissues of interest. However, I started out as an animal person so love
lots of "stuff" in my images.

Many plant cells have huge internal vacuoles and relatively few organelles
so can be very uninteresting to many people. However, if you start out with
very young tissue, such as the very young leaves from a bean plant, than you
will find lots of interesting stuff.

In this case, the leaves will be so thin that you will be able to prepare
and microtome x-sections through the entire leaf. You will see the
different cell types and arrangements toward both the top and bottom leaf
portions. You will also get lots of chloroplasts, nuclei, a fair number of
mitochondria and other organelles. You should be able to find golgi, etc.
If you are lucky you will cut through stomata as well.

This can be lots of fun as well as informative. Good luck!

I would recommend a half strength Karnovsky fixative in Cacodylate buffer
followed by osmium and possibly en block uranyl acetate staining. You might
need something to control the osmolarity such as NaCl. Check the literature
for details. Embedding should be in Spurr resin or other low viscosity resin
as plant walls can be difficult to infiltrate. Use a very gradual
infiltration, increasing resin concentration very slowly with gentle
rotation/agitation until you have reached 50% resin and then can go a bit
faster.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


On 9/7/05 3:30 AM, "ishask2aol.com-at-ns.microscopy.com"
{ishask2aol.com-at-ns.microscopy.com} wrote:

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} at 00:24:01
} ---------------------------------------------------------------------------
}
} Email: ishask2aol.com
} Name: isha
}
} Organization: san joaquin delta college, electron microscopy program
}
} Title-Subject: [Filtered] fixing and viewing plant on tem
}
} Question: i am a EM student at san joaquin delta college in CA. we
} have a project coming up where we have to fix, section, stain and
} view plant tissue on the tem.
} so i was wondering which part of a plant will be interesting to look
} at...or is there a particular plant that i should look for
} thanks
} isha
}
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 8, 12 -- From zaluzec-at-microscopy.com Wed Sep 7 03:27:56 2005
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==============================Original Headers==============================
12, 21 -- From dsherman-at-purdue.edu Wed Sep 7 10:41:04 2005
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From: glenmac-at-u.washington.edu
Date: Wed, 7 Sep 2005 11:09:50 -0500
Subject: [Microscopy] Re: Administrivia: Replies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor,
Is there any way to make 'Reply All' include the listserver address
along with the correspondent's address?

It will be interesting to see if mis-addressed replies increase on
the other microscopy-related listservers that retain the old reply
mechanism.

Regards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-u.washington.edu

************************************************************************
******
The box said "Requires Windows 95 or better", so I bought a Macintosh.
************************************************************************
******


On Sep 7, 2005, at 1:23 AM, zaluzec-at-microscopy.com wrote:

}
}
}
} ----------------------------------------------------------------------
} ------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
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} MicroscopyListserver
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} ----------------------------------------------------------------------
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}
} Greg etal....
}
} I concur with you Greg, I personally prefer the old method.
} However, the change was made about a month ago
} as we were getting far too many inadvertent postings
} and the rash that occurred at the end of July was getting just
} too much, particularly with the almost immediate
} followup of an apology.
}
} For the time being, just a reminder to all subscribers.
} Please remember (and this is documented in the Instructions
} if you are replying to a posting and consider the information of
} general use to everyone, remember to change the reply to address
} from the senders address (automatically provided by your Email
} client) to include
}
} microscopy-at-microscopy.com
}
}
} It is also appropriate should an individual collects a series of
} answers
} /solutions to their question that (s)he compose a summary of
} the responses and post that back to the list. This way it will be
} included in the
} archives and provide a convenient mechanism for others to
} locate a synopsis of the question and answers using the search
} engine
}
} Cheers
}
} Nestor
} Your Friendly Neighborhood SysOp
}
}
}
}
}
} At 9:41 AM -0500 9/6/05, gwe-at-ufl.edu wrote:
}
} } ---------------------------------------------------------------------
} } -------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
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} } MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
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} } Does anyone else miss seeing the answers to questions. Now that
} } replies
} } do not go to the list we are missing out on a lot. Folks need to
} } remember to add the list to their replies, unless the reply is
} } intended
} } only for the original sender
} }
} } --
} } Gregory W. Erdos, Ph.D.
} } Assistant Director, Biotechnology Program
} } Scientific Director, Electron Microscopy
} } P.O. Box 118525
} } 217 Carr Hall
} } University of Florida
} } Gainesville, FL 32611
} } Phone: 352-392-1295
} } Fax: 352 846 0251
} }
} }
} }
} }
}
} ==============================Original
} Headers==============================
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From: Sue.Tyler-at-noaa.gov
Date: Wed, 7 Sep 2005 13:16:56 -0500
Subject: [Microscopy] Clarification of Marine TEM fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I need to clarify my fixation question. First, I am trying to fix marine
amphipods for both light and TEM. Currently I am fixing them in 2.5%
Gluteraldehyde and 2.5% Formaldehyde in sodium cacodylate buffer and
bisecting the animal and placing half in 70% EtOH for light microscopy
and the other half in cacodylic buffer. I then process the animals for
light and if the pathologist sees anything interesting I will have the
other half of the animal fixed for any em work. I am not doing any immuno.

The fixation is fine, but is there a less labor intensive way? I don't
like storing my tissue in the buffer because it molds after about 8 months.

I appreciate all of your questions and I look forward to your comments.

Sue

==============================Original Headers==============================
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4, 18 -- Message-ID: {431F2E97.8000101-at-noaa.gov}
4, 18 -- Date: Wed, 07 Sep 2005 14:16:55 -0400
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From: Sue.Tyler-at-noaa.gov
Date: Wed, 7 Sep 2005 14:28:11 -0500
Subject: [Microscopy] Re: Clarification of Marine TEM fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ken Tiekotter wrote:

} Sue,
}
} I misplaced your reply to me, however, I must ask a couple more questions.
} I assume you are processing the 70% half into paraffin and the other half
} goes into resin.
}
} Rather than process all tissues in plastic, I assume the material needs to
} be scanned by the pathologist with the light microscope. If he or she finds
} areas of interest, you are then required to process the other half for
} ultrastructural TEM examination: ok so far?
}
} Are you specifically embedding separately because of the size of the
} amphipods or because you are required to stain with either H&E or other more
} specific stains.
}
} If it is a size related issue, you will more than likely need to process
} both halves to paraffin and resin.
}
} If it is a stain related issue, you may want to try embedding in LR White
} methylmethacrylate resin. This resin is quite different from epoxy resins
} in that you can use a acid fuchsin/methylene blue stain to produce an H&E
} like stained section.
}
} If you pathologist is not too concerned with leaching of material over time,
} you might be better off leaving your specimens in your primary fixative
} instead of cacodylate buffer. If you are getting growth in your buffer over
} time, you must be stored for several months. Even this length of time will
} leach proteins.
}
} I asked about your use or if you use a digital ccd camera on your TEM
} because the ccd will provide incredible contrast. I used to use a
} glutaraldehyde/osmium/tannic acid/osmium fixative for contrast and
} penetration. With the digital camera, I don't need the extra contrast.
}
} Regards,
} Ken
} _______________________________________
} Kenneth L. Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N Willamette Blvd.
} Portland, OR 97203 USA
}
} Tel.: 503.943.8861
} Email: tiekotte-at-up.edu
}
}
} On 9/7/05 11:17 AM, "Sue.Tyler-at-noaa.gov" {Sue.Tyler-at-noaa.gov} wrote:
}
}
}
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
2, 19 -- From sue.tyler-at-noaa.gov Wed Sep 7 14:28:10 2005
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2, 19 -- Message-ID: {431F3F49.2020200-at-noaa.gov}
2, 19 -- Date: Wed, 07 Sep 2005 15:28:09 -0400
2, 19 -- From: "Sue Tyler" {Sue.Tyler-at-noaa.gov}
2, 19 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317)
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2, 19 -- Subject: Re: [Microscopy] Clarification of Marine TEM fixative
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From: glaevsky-at-ECE.NEU.EDU
Date: Thu, 8 Sep 2005 08:42:44 -0500
Subject: [Microscopy] Research and Industrial Collaboration Conference (RICC)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues



I am writing to invite you to attend a day long symposium on advanced
biomedical imaging techniques. The venue for this is the annual Research
and Industrial Collaboration Conference (RICC) sponsored by CenSSIS and
held at Northeastern University in Boston. The RICC is a two day affair
(October 6‑7). The biomedical imaging symposium will take place on
Friday, October 7th (Day Two of the RICC). The morning session of the
symposium will discuss stem cell imaging and will include speakers such as
Gerald Schatten (a member of the team that reported the recent canine
cloning in South Korea). The afternoon will focus upon techniques for multi
spectral cellular imaging and will include speakers from both academia and
industry.



A complete agenda listing, directions and registration form can be found on
the web link:
{http://www.censsis.neu.edu/RICC/2005} http://www.censsis.neu.edu/RICC/2005.



A more general description of the CenSSIS research and education programs
can be found on our homepage:
{http://www.censsis.neu.edu/} http://www.censsis.neu.edu.



In addition to the presentations at the symposium, there will be an
opportunity to learn about the 3D Keck Fusion Microscope (3DFM) facility
which is located at Northeastern. The Keck 3DFM is a multi-modal microscope
capable of imaging live samples in up to six different modes for at least
72 hours. Along with standard DIC and epifluorescence modes, we are imaging
with single and two photon fluorescent excitation wavelengths from 454nm up
to 1100nm. We also have a newly patented, noninvasive, and nontoxic imaging
mode called Quadrature Microscopy. This specialized technique measures the
phase change of light as it passes through a live specimen and yields
comprehensive real time data. A link to the full description of the
facility can be found on the CenSSIS homepage.



I hope that you will be able to attend our symposium. Don't hesitate to
contact me if you need further information. The registration deadline is
Sunday, October 1st.







**Important Note: All unused rooms will be released from the hotel room
blocks on September 14, 2005. Please be sure to make your reservation as
soon as possible. Room price and availability cannot be guaranteed beyond
these dates.

Best,

Gary



Gary Laevsky, Ph.D.
Keck Facility Manager, CenSSIS
Northeastern University
302 Stearns
360 Huntington Ave.
Boston, MA 02115
voice(617) 373 - 2589 {br}
fax(617) 373 - 7783 {br} {br}

http://www.censsis.neu.edu

http://www.ece.neu.edu/groups/osl

http://www.keck3dfm.neu.edu



==============================Original Headers==============================
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From: gwe-at-ufl.edu
Date: Thu, 8 Sep 2005 14:02:51 -0500
Subject: [Microscopy] Tutorials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear MSA members and other listers,
As wrangler of the MSA video collection I am often asked if we have
a full basic course in electron microscopy available on DVD. We do
not. I can also infer from folks who purchase 20 or 30 or 40 or 50
DVDs that they intend to teach themselves what they need to know from
our recorded tutorials. (The record purchase is 52 DVDs that was filled
last month.). In light of the fact that EM coures are disappearing at
universities around the country, the Education Committee of MSA is
considering the idea of putting together a basic course or courses on
electron microscopy and make them available on DVD. Something that
would present the technology in an organized fashion and be
comprehensive enough to put a student in a position to begin work in the
area. If such a project is undertaken, Steve Barlow and Howard Berg
have agreed to work with me on coordinating it.
First, we would like to hear from the community on whether or not
they think this is a good idea. Second we would be looking for
volunteers who might be willing to record a lesson with supporting
demonstrations and other visual representations that could flesh out the
coverage. We don't want just a talking head. We would also be looking
for folks who would be willing to share the syllabi from their courses
so that we might determine how to go about organizing such a thing.
Any and all feedback is welcome.

Regarsd to all,
Greg




--
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
Scientific Director, Electron Microscopy
P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
Phone: 352-392-1295
Fax: 352 846 0251



==============================Original Headers==============================
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From: Sue.Tyler-at-noaa.gov
Date: Thu, 8 Sep 2005 14:19:45 -0500
Subject: [Microscopy] Re: Tutorials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Greg-
Yea! I was on of those who purchased your DVD's and yes, I would love to
have a DVD with the basic course. It would be great if you could
improve the DVD quality, the info was great, the visual could be better.
Good Idea! I hope you get a lot of response from the EM community.

Sue
New User!








gwe-at-ufl.edu wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
14, 19 -- From sue.tyler-at-noaa.gov Thu Sep 8 14:19:45 2005
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From: astamand-at-phycotech.com
Date: Thu, 8 Sep 2005 14:23:09 -0500
Subject: [Microscopy] service contracts on SEM's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, I'm researching SEM instruments and am wondering about yearly service
contracts and how they change over the life of the
instrument. Thoughts? Thanks, Ann.

Ann St. Amand, Ph.D., CLP
President
PhycoTech, Inc.
620 Broad St., Suite 100
St. Joseph, Michigan 49085 USA

269-983-3654 (voice)
269-983-3653 (fax)
mailto:astamand-at-phycotech.com
www.phycotech.com

Raising the Standard in Aquatic Sample Analyses

Secretary, North American Lake Management Society, www.nalms.org


==============================Original Headers==============================
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From: SHem-at-laurentian.ca
Date: Thu, 8 Sep 2005 14:38:18 -0500
Subject: [Microscopy] Silicate Standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello There
Anyone know a good source for Silicate standards
for EDS/WDS analysis ?

Any advice is greatly appriciated.
Thanks,
Skage

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-675-1151 x4040
cell. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html



==============================Original Headers==============================
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From: henriks-at-southbaytech.com
Date: Thu, 8 Sep 2005 18:02:24 -0500
Subject: [Microscopy] Re: Silicate Standards

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Skage:

You may want to try Geller MicroAnalytical: http://www.gellermicro.com/

Best regards-

David

--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.




SHem-at-laurentian.ca wrote:

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From: walck-at-southbaytech.com
Date: Thu, 8 Sep 2005 18:16:27 -0500
Subject: [Microscopy] re: Tutorials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think that that would be a great idea. However, I have another suggestion to add to it. I would like to see the original lesson broadcast over the internet where MSA members could "tune-in" to watch. Perhaps there could be a question and answer period from audience memebers that wold be included int he video also. Afterall, many of the original tapes were recorded at the meetings.



-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: +1-949-492-2600
FAX: +1-949-492-1499
walck-at-southbaytech.com

-------- Original Message --------
} From: gwe-at-ufl.edu
} Sent: Thursday, September 08, 2005 3:09 PM
} To: Walck-at-SouthBayTech.com
} Subject: [Microscopy] Tutorials
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} ----------------------------------------------------------------------------
}
} Dear MSA members and other listers,
} As wrangler of the MSA video collection I am often asked if we have
} a full basic course in electron microscopy available on DVD. We do
} not. I can also infer from folks who purchase 20 or 30 or 40 or 50
} DVDs that they intend to teach themselves what they need to know from
} our recorded tutorials. (The record purchase is 52 DVDs that was filled
} last month.). In light of the fact that EM coures are disappearing at
} universities around the country, the Education Committee of MSA is
} considering the idea of putting together a basic course or courses on
} electron microscopy and make them available on DVD. Something that
} would present the technology in an organized fashion and be
} comprehensive enough to put a student in a position to begin work in the
} area. If such a project is undertaken, Steve Barlow and Howard Berg
} have agreed to work with me on coordinating it.
} First, we would like to hear from the community on whether or not
} they think this is a good idea. Second we would be looking for
} volunteers who might be willing to record a lesson with supporting
} demonstrations and other visual representations that could flesh out the
} coverage. We don't want just a talking head. We would also be looking
} for folks who would be willing to share the syllabi from their courses
} so that we might determine how to go about organizing such a thing.
} Any and all feedback is welcome.
}
} Regarsd to all,
} Greg
}
}
}
}
} --
} Gregory W. Erdos, Ph.D.
} Assistant Director, Biotechnology Program
} Scientific Director, Electron Microscopy
} P.O. Box 118525
} 217 Carr Hall
} University of Florida
} Gainesville, FL 32611
} Phone: 352-392-1295
} Fax: 352 846 0251
}
}
}
} ==============================Original Headers==============================
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From: cgarber-at-2spi.com
Date: Thu, 8 Sep 2005 20:11:10 -0500
Subject: [Microscopy] Availability of silicate standards for microanalysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Skage Hem wrote:
=====================================================
Anyone know a good source for Silicate standards for EDS/WDS analysis ?

Any advice is greatly appriciated.
=====================================================
SPI Supplies offers a number of silicate containing standards, from both the
SPI Supplies and also the Charles M. Taylor standards collection. Go to URL
http://www.2spi.com/catalog/standards/aweb/index.html
and on the Periodic Table of the Elements, click on Si and then you will get
a display of all the silicon containing standards including of course those
that are silicates.

These standard items are all all stock and can be offered as both loose
standards or mounted in a finished "block" complete with electron beam
labeling, Faraday cup and instruction manual.

Disclaimer: SPI Supplies is a supplier of standards for microanalysis.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




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From: lahec-at-wcoil.com
Date: Fri, 9 Sep 2005 02:46:32 -0500
Subject: [Microscopy] AskAMicroscopist: interactice/ creative microscopy lessons

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (lahec-at-wcoil.com) from
http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
on Thursday, September 8, 2005 at 15:32:34
---------------------------------------------------------------------------

Email: lahec-at-wcoil.com
Name: Maggie Turnbull

Organization: Lima Area Health Education Center

Education: 6-8th Grade Middle School

Location: City, State, Country

Question: I'm interested in finding highly interactice/ creative
microscopy lessons.
tx, mt

---------------------------------------------------------------------------

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7, 12 -- To: microscopy-at-microscopy.com
7, 12 -- From: lahec-at-wcoil.com (by way of Ask-A-Microscopist)
7, 12 -- Subject: AskAMicroscopist: interactice/ creative microscopy lessons
7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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From: pgan-at-ap.ansell.com
Date: Fri, 9 Sep 2005 02:54:18 -0500
Subject: [Microscopy] viaWWW: shelf life of the conductive paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (pgan-at-ap.ansell.com) from
http://microscopy.com/MLFormMail.html on Thursday, September 8, 2005
at 18:48:33
---------------------------------------------------------------------------

Email: pgan-at-ap.ansell.com
Name: Phay Fang Gan

Organization: Ansell

Title-Subject: [Filtered] Shelf Life

Question: May I know the shelf life of the conductive silver paint
and carbon paint ?

Thanks

---------------------------------------------------------------------------

==============================Original Headers==============================
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7, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed"
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From: kevin.braeckmans-at-ugent.be
Date: Fri, 9 Sep 2005 03:07:21 -0500
Subject: [Microscopy] RE: AskAMicroscopist: interactice/ creative microscopy lessons

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Maggie,

You may want to try out these great interactive websites:

http://www.microscopyu.com/
http://www.olympusmicro.com/

They actually contain the same interactive material, but adapted for the
microscopes of Nikon and Olympus, respectively.

Best regards,

Kevin Braeckmans



} -----Oorspronkelijk bericht-----
} Van: lahec-at-wcoil.com [mailto:lahec-at-wcoil.com]
} Verzonden: vrijdag 9 september 2005 9:55
} Aan: kevin.braeckmans-at-ugent.be
} Onderwerp: [Microscopy] AskAMicroscopist: interactice/
} creative microscopy lessons
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
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}
} Below is the result of your feedback form (NJZFM-ultra-55).
} It was submitted by (lahec-at-wcoil.com) from
} http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html
} on Thursday, September 8, 2005 at 15:32:34
} --------------------------------------------------------------
} -------------
}
} Email: lahec-at-wcoil.com
} Name: Maggie Turnbull
}
} Organization: Lima Area Health Education Center
}
} Education: 6-8th Grade Middle School
}
} Location: City, State, Country
}
} Question: I'm interested in finding highly interactice/
} creative microscopy lessons.
} tx, mt
}
} --------------------------------------------------------------
} -------------
}
} ==============================Original
} Headers==============================
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} way of Ask-A-Microscopist) 7, 12 -- Subject:
} AskAMicroscopist: interactice/ creative microscopy lessons 7,
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}


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From: a.boyde-at-qmul.ac.uk
Date: Fri, 9 Sep 2005 03:20:58 -0500
Subject: [Microscopy] One day image analysis meeting, London England, Sept 19

Contents Retrieved from Microscopy Listserver Archives
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http://www.anatsoc.org.uk/

Image acquisition, processing and analysis in biomedical research

This symposium is organised by Daniel Zicha (Cancer Research UK) and Alan
Boyde (Queen Mary University of London). The scientific sessions will take
place in the Dent Room, Student Union Building, 43-46 Huntley Street,
London WC1.
or if the number of attendees is too great, in the Anatomy Department,
University College London, Gower St. Late registration is still available
for £10 (including refreshments) but intending registrants should email the
Programme Secretary (jonathan.bennett-at-hyms.ac.uk) to indicate their
intention of attending.


Alan Boyde, Biophysics Section, Centre for Oral Growth and Development,
QMUL,
Dental Institute, New Rd., London E1 1BB, UK
{a.boyde-at-qmul.ac.uk}


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From: richard.beanland-at-bookham.com
Date: Fri, 9 Sep 2005 04:26:03 -0500
Subject: [Microscopy] viaWWW: shelf life of the conductive paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I can't answer about graphite paint, but I am still using the same bottle of colloidal silver after 10 years. I'm a TEM (AFM,XRD,FIB...) man really so I only use it to mount samples for SEM once in a while. Even if it's completely dried out I find that adding a bit of the relevant solvent (4-Metyl-pentan-2-one, usually) and a buzz in an ultrasonic bath for an hour makes it as good as new. So I would call the shelf life "unlimited". The main problem is getting the damn lid off the bottle..

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


-----Original Message-----
X-from: pgan-at-ap.ansell.com [mailto:pgan-at-ap.ansell.com]
Sent: 09 September 2005 08:57
To: Richard Beanland

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (pgan-at-ap.ansell.com) from
http://microscopy.com/MLFormMail.html on Thursday, September 8, 2005
at 18:48:33
---------------------------------------------------------------------------

Email: pgan-at-ap.ansell.com
Name: Phay Fang Gan

Organization: Ansell

Title-Subject: [Filtered] Shelf Life

Question: May I know the shelf life of the conductive silver paint
and carbon paint ?

Thanks

---------------------------------------------------------------------------

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From: wesaia-at-iastate.edu
Date: Fri, 9 Sep 2005 08:33:40 -0500
Subject: [Microscopy] Re: viaWWW: shelf life of the conductive paint

Contents Retrieved from Microscopy Listserver Archives
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-------- Original Message --------

I would say much the same as Richard Beanland but about carbon paint. We
have had jars sitting from a large order sitting on the shelf for years.
They have always been fine when I open a new one.

Our big problem is getting users to put the caps back on tightly. A couple
times a month I go around our lab and check the opened bottles and add
propanol to bring them back to the original consistency. Time in the sonic
batch would probably help, but it will just dry out again. I usually give
it a good shake, let it sit for a while and shake it again. Even with that,
I usually rarely get a chance to through a bottle away because it's empty.
Usually, I find one that has dried out too much before I get to it and
figure it is not worth the hassle to try to rejuvenate it.

Warren Straszheim
Iowa State University

At 02:56 AM 09/09/05, you wrote:

} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (pgan-at-ap.ansell.com) from
} http://microscopy.com/MLFormMail.html on Thursday, September 8, 2005
} at 18:48:33
} ---------------------------------------------------------------------------
}
} Email: pgan-at-ap.ansell.com
} Name: Phay Fang Gan
}
} Organization: Ansell
}
} Title-Subject: [Filtered] Shelf Life
}
} Question: May I know the shelf life of the conductive silver paint
} and carbon paint ?
}
} Thanks


==============================Original Headers==============================
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From: dwaugh-at-kent.edu
Date: Fri, 9 Sep 2005 09:07:43 -0500
Subject: [Microscopy] 3D CT Reconstruction Software

Contents Retrieved from Microscopy Listserver Archives
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A question for the list: I am looking for some (cheap/easy to use) 3D
reconstruction software to make 3D models from CT slices. I'm not
looking for anything to fancy. I'm looking at CT data from extant
crab claws and need to model an internal structure that is more
dense, and show it in relation to the exterior of the claw. The two
programs I have looked at are Surfdriver (now only for PC) and
VGStudio Max. Does anyone have any other software options I should be
looking at? I'm hoping the software would work on the Mac, but if I
had to I could find a PC. Many thanks in advance.
-David
--
David A. Waugh
Kent State University
Department of Geology
Kent, Ohio 44242
dwaugh-at-kent.edu
Http://www.personal.kent.edu/~dwaugh/

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From: jbs-at-temple.edu
Date: Fri, 9 Sep 2005 09:15:05 -0500
Subject: [Microscopy] Re: 3D CT Reconstruction Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Depending on the sophistication of the reconstruction you need, you
should check out ImageJ, which is available free at:
http://rsb.info.nih.gov/ij/. The program is written in Java, and
will run on virtually any platform.


}
}
}
} ----------------------------------------------------------------------
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}
}
} A question for the list: I am looking for some (cheap/easy to use) 3D
} reconstruction software to make 3D models from CT slices. I'm not
} looking for anything to fancy. I'm looking at CT data from extant crab
} claws and need to model an internal structure that is more dense, and
} show it in relation to the exterior of the claw. The two programs I
} have looked at are Surfdriver (now only for PC) and VGStudio Max. Does
} anyone have any other software options I should be looking at? I'm
} hoping the software would work on the Mac, but if I had to I could
} find a PC. Many thanks in advance. -David -- David A. Waugh Kent State
} University Department of Geology Kent, Ohio 44242 dwaugh-at-kent.edu
} Http://www.personal.kent.edu/~dwaugh/
}
} ==============================Original
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Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


==============================Original Headers==============================
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From: paul_hazelton-at-umanitoba.ca
Date: Fri, 9 Sep 2005 09:35:51 -0500
Subject: [Microscopy] reduced osmium fixation for membranes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

OK people. I've tried to send this twice already, and had some problems
which do not appear evident to me. But will try again.

I would like to try and get a thread going on the subject of reduced
osmium fixation. Not so much on the ‘what I’ve used’ form as the ‘what
is the difference, what is the value, what is the mechanism’ form. Some
hypothetical, perhaps our more chemically inclined can provide some
factual information on chemical reactions, and some ‘what protocols
exist’ form. Gee, I guess that last point does get into the what 'I’ve
used' form, doesn’t it. OK, include the 'what I've used'.

The source of interest which has given rise to this is a question a
student asked the other day. This person had ferrocyanide (Fe4+) and
wanted to know if it were possible to use it in place of ferricyanide
(Fe3+). Since my procedure called for ferricyanide, and I had some, I
gave it to them and they went away happy. At least I think they were
happy, I never can really tell about students. Then there was the
listing from Richard Berry in Australia, and Geoff McAuliff's response
which also raised Osmium-ferricyanide.

Unfortunately, this has all started me thinking - always a dangerous
thing. So, I checked my copy of Hayat’s Fixation for Electron
Microscopy [sorry Phil, some of us do have it ;-)]. The results were
quite interesting.

Hayat mentioned procedures for both ferri- and ferrocyanide. Went to
his original references, read those i could get my hands on readily (our
library has taken to storing some older issues of journals). I won’t
get into a long re-hash of what is there. Briefly: 1.) The original
hypothesis by Elbers was that fixation with ferricyanide/lead as a step
between glut and osmium, could help stabilize phospholipids.
Subsequently it was used to stabilize surfactants. Maybe stabilize
surfactants - there is mixed data on that. The key seemed to be the
presence of the Pb, not the Fe. 2.) There was a ferricyanide report
that used 1.6% ferricyanide, 1% Osmium. Note, this is 2x the
concentration of ferricyanide I use now. Hayat didn't really say what
the advantage was supposed to be, though.. 3.) Karnovsky’s report to
the 11th (or 14th) meeting of the Am Soc. for Cell Biology in 1970, and
the Russell & Burguet work from 1977 were discussed, but the roles in
membrane fixation, or mechanism, for that matter, were not. This is also
covered in Bozzolo and Russell under membrane fixation. 4.) de Bruijn
and Den Breejen’s work (1975) which showed no difference in ferricyanide
and ferrocyanide reduced Os in terms of subsequent staining of glycogen
was referenced, but not discussed.

Next, I checked the catalogues to see what the EM suppliers made
available. That's always a good indicator as to what people are using.
Some suppliers had ferricyanide, some didn't. And i found none with
ferrocyanide. Some of our regular suppliers of non EM lab supplies,
chemicals, etc, do sell both, and as 10% solutions, which makes using it
real easy!!!

So, for the thread. Is there a difference between ferri- and
ferrocyanide. If there are any differences, why? Is the operative
agent iron, as a reducer of osmium from VIII to VI, or is it a mordant
effect of the cyanide moieties - as suggested by some? What
concentration of ferri/ferrocyanide should we use, 0.8%, 1.5%, or even
2.5% as recommeded by Russell and Burguet?

Today, my only thought is that K4Fe(CN)6 would reduce the Os from
octavalent to hexavalent more quickly than K3Fe(CN)6, but I doubt it
would make a practical difference in the fixative.

Any takers on seeing if we can educate ourselves on this. Get a good,
profitable discussion on line?

paul


paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926


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From: colijn.1-at-osu.edu
Date: Fri, 9 Sep 2005 10:21:16 -0500
Subject: [Microscopy] Imaging Plates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

We are looking into the possibility of replacing our film cameras with
Imaging Plates. I am aware of the Fuji and Ditabis systems. Are there any
other systems available? (vendors welcome to respond!)

I would also appreciate any feedback concerning the use of the IP systems,
both concerning the particular vendor/system and IP units in general. It
is probably best to keep the feedback off-line. I can summarize to the
list later.

Thanks,
Henk


Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://www.ceof.ohio-state.edu
Time is that quality of nature which keeps events from happening all at
once. Lately it doesn't seem to be working.


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From: jfactor-at-ns.purchase.edu
Date: Fri, 9 Sep 2005 10:34:25 -0500
Subject: [Microscopy] Re: viaWWW: shelf life of the conductive paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I subdivide a new bottle of colloidal graphite into several small vials
(usually liquid scintilation vials) and cap them tightly. This way, as
the vial in use dries out (or gets left uncapped), I don't lose the
entire stock.
--Jan Factor

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College, State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



pgan-at-ap.ansell.com wrote:

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From: jehrman-at-mta.ca
Date: Fri, 9 Sep 2005 11:00:39 -0500
Subject: [Microscopy] viaWWW: shelf life of the conductive paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Phay Fang Gan,

We tell our customers one year unopened, six months after being opened,
however.we know of people keeping and using much longer than that. If you
have any further questions or want to discuss this with someone, our chemist
Dr. Charles Duvic would be happy to speak with you. Please call or e-mail
him at the numbers listed below.

JD Arnott

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: ladres-at-att.net


********************
Disclaimer: Ladd Research sells Supplies for microscopy such as conducting
paints listed in this e-mail.
********************

----- Original Message -----
X-from: {pgan-at-ap.ansell.com}
To: {ladres-at-worldnet.att.net}
Sent: Friday, September 09, 2005 3:59 AM

One thing that seems to help prolong the usability of carbon paint is to
shake the bottle *after* you're through
using it. This helps wash the semi-dried material at the neck back into
solution, so you don't get as much
dried up gunk in the neck of the bottle after repeated use. This is
especially true if the cap has a brush built
in, and you use the edge of the bottle to slurp off the excess before
applying to stubs. Just make sure the bottle
is tightly closed (speaking from experience).

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf



wesaia-at-iastate.edu wrote:

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From: glenmac-at-u.washington.edu
Date: Fri, 9 Sep 2005 11:43:48 -0500
Subject: [Microscopy] Re: 3D CT Reconstruction Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear David,
Take a look at Osirix. http://homepage.mac.com/rossetantoine/osirix/

Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-u.washington.edu

************************************************************************
******
The box said "Requires Windows 95 or better", so I bought a Macintosh.
************************************************************************
******


On Sep 9, 2005, at 7:09 AM, dwaugh-at-kent.edu wrote:

}
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}
} A question for the list: I am looking for some (cheap/easy to use) 3D
} reconstruction software to make 3D models from CT slices. I'm not
} looking for anything to fancy. I'm looking at CT data from extant
} crab claws and need to model an internal structure that is more
} dense, and show it in relation to the exterior of the claw. The two
} programs I have looked at are Surfdriver (now only for PC) and
} VGStudio Max. Does anyone have any other software options I should be
} looking at? I'm hoping the software would work on the Mac, but if I
} had to I could find a PC. Many thanks in advance.
} -David
} --
} David A. Waugh
} Kent State University
} Department of Geology
} Kent, Ohio 44242
} dwaugh-at-kent.edu
} Http://www.personal.kent.edu/~dwaugh/
}
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From: dsherman-at-purdue.edu
Date: Fri, 9 Sep 2005 12:51:27 -0500
Subject: [Microscopy] Need IgE conjugate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Does anyone have a source for colloidal gold (preferably 10nm) conjugated to
human IgE? We are trying to identify the source of an allergen so the right
conjugate is rather critical.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



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6, 19 -- Subject: Need IgE conjugate
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From: peoshel-at-wisc.edu
Date: Fri, 9 Sep 2005 13:36:25 -0500
Subject: [Microscopy] Re: Clarification of Marine TEM fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sue,

An impressive mold, growing in an arsenical compound.
I suspect there is no less labor intensive method. I certainly never
found an easier method when I was doing amphipods. The best way to
store the EM specimens is to go ahead and process and embed them.
Once they're in plastic, they'll last for years. With no mold.
The good fixation is more important than less labor.

Phil

} I need to clarify my fixation question. First, I am trying to fix marine
} amphipods for both light and TEM. Currently I am fixing them in 2.5%
} Gluteraldehyde and 2.5% Formaldehyde in sodium cacodylate buffer and
} bisecting the animal and placing half in 70% EtOH for light microscopy
} and the other half in cacodylic buffer. I then process the animals for
} light and if the pathologist sees anything interesting I will have the
} other half of the animal fixed for any em work. I am not doing any immuno.
}
} The fixation is fine, but is there a less labor intensive way? I don't
} like storing my tissue in the buffer because it molds after about 8 months.
}
} I appreciate all of your questions and I look forward to your comments.
}
} Sue


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From: bozzola-at-siu.edu
Date: Fri, 9 Sep 2005 13:51:35 -0500
Subject: [Microscopy] temperature of 100 kV beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A colleague, who is experiencing specimen damage in the TEM, inquired
if anyone knew the temperature generated on the specimen by the
electron beam. I realize that there are a lot of variables here, but
even a range of temperatures would be useful.

Here are his operating parameters:


kV = 100
spot size = 2 micrometer (specimen was examined at approximately 2
micrometer spot size)
specimen = ceramic containing metal particles (Ti)
magnification = 100K to 600K
electron gun = LaB6
vacuum = turbo pumped to 10-5 Pa
substrate that specimen was mounted on = Formvar/carbon coated grid


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################

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From: tivol-at-caltech.edu
Date: Fri, 9 Sep 2005 14:31:26 -0500
Subject: [Microscopy] Re: temperature of 100 kV beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Sep 9, 2005, at 11:51 AM, bozzola-at-siu.edu wrote:

} A colleague, who is experiencing specimen damage in the TEM, inquired
} if anyone knew the temperature generated on the specimen by the
} electron beam. I realize that there are a lot of variables here, but
} even a range of temperatures would be useful.
}
} Here are his operating parameters:
}
}
} kV = 100
} spot size = 2 micrometer (specimen was examined at approximately 2
} micrometer spot size)
} specimen = ceramic containing metal particles (Ti)
} magnification = 100K to 600K
} electron gun = LaB6
} vacuum = turbo pumped to 10-5 Pa
} substrate that specimen was mounted on = Formvar/carbon coated grid
}
Dear John,
Unfortunately, I don't have one of my most useful references with me
to get an essential parameter, but the general method of doing this
calculation is to use the stopping power of the material to determine
the energy deposited into the specimen, then calculate the temperature
increase from the heat capacity and account for conduction and
radiation of heat. At steady state, the heat in, which is the stopping
power, dE/dx, in units of joules/meter times the electron beam current
times the specimen thickness, must equal the sum of conduction (assume
a disk at one temperature surrounded by an infinite amount of the
material at ambient temperature, plug in the conductivity, the
temperature difference, and the area across which the heat is
conducted, which is the circumference of the beam spot times the
thickness of the specimen) and radiation, which is equal to T^4 (on the
Kelvin scale) times the area of the beam times the Stephan-Boltzman
constant. The stopping power can be set equal to the sum of stopping
powers for each element in the specimen times their fractions. The
effect of the grid can probably be ignored (unless the illuminated part
of the specimen is over a grid bar, which would greatly increase heat
conduction). The parameters necessary to do the calculation are the
stopping powers, the heat conductivity, and the geometry of the
specimen. Then one can set heat in = heat out and solve for the
temperature for which the equation holds.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: tivol-at-caltech.edu
Date: Fri, 9 Sep 2005 15:20:53 -0500
Subject: [Microscopy] Re: temperature of 100 kV beam, Addendum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Sep 9, 2005, at 11:51 AM, bozzola-at-siu.edu wrote:
}
} } A colleague, who is experiencing specimen damage in the TEM, inquired
} } if anyone knew the temperature generated on the specimen by the
} } electron beam. I realize that there are a lot of variables here, but
} } even a range of temperatures would be useful.
} Dear John,
} At steady state, the heat in, which is the stopping power, dE/dx, in
} units of joules/meter times the electron beam current times the
} specimen thickness, ...

This gives an upper limit to the heat deposited in the specimen, since
not all the energy loss is converted to heat. Some is carried away by
bremsstrahlung, secondary electrons, etc.

} Then one can set heat in = heat out and solve for the temperature for
} which the equation holds.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: MSHERWOOD-at-PARTNERS.ORG
Date: Fri, 9 Sep 2005 15:33:37 -0500
Subject: [Microscopy] Re: Clarification of Marine TEM fixative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sue,

.....If it is a stain related issue, you may want to try embedding in LR White
methylmethacrylate resin. This resin is quite different from epoxy resins in
that you can use a acid fuchsin/methylene blue stain to produce an H&E like
stained section...

I have used (successfully) another "H & E"-like stain with epon. My reference
is an application note (303) from LKB: "Stains for Plastic Embedded Tissue
Sections II. Staining of sections from different animal, human and plant
tissues with a methylene blue-azure II-basic fuchsin stain" (Humphrey) - Maj
Andersson (April 1977).

The original reference was: Humphrey, CD and Pittman, FE (1974) "A simple
methylen blue-azure II-basic fuchsin stain for epoxy-embedded tissue sections"
Stain Technology. 49; 9-14.

It gives beautiful results.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org

One clarification: there is another

-----Original Message-----
X-from: Sue.Tyler-at-noaa.gov [mailto:Sue.Tyler-at-noaa.gov]
Sent: Wednesday, September 07, 2005 3:33 PM
To: Sherwood, Margaret

Ken Tiekotter wrote:

} Sue,
}
} I misplaced your reply to me, however, I must ask a couple more questions.
} I assume you are processing the 70% half into paraffin and the other half
} goes into resin.
}
} Rather than process all tissues in plastic, I assume the material needs to
} be scanned by the pathologist with the light microscope. If he or she finds
} areas of interest, you are then required to process the other half for
} ultrastructural TEM examination: ok so far?
}
} Are you specifically embedding separately because of the size of the
} amphipods or because you are required to stain with either H&E or other more
} specific stains.
}
} If it is a size related issue, you will more than likely need to process
} both halves to paraffin and resin.
}
} If it is a stain related issue, you may want to try embedding in LR White
} methylmethacrylate resin. This resin is quite different from epoxy resins
} in that you can use a acid fuchsin/methylene blue stain to produce an H&E
} like stained section.
}
} If you pathologist is not too concerned with leaching of material over time,
} you might be better off leaving your specimens in your primary fixative
} instead of cacodylate buffer. If you are getting growth in your buffer over
} time, you must be stored for several months. Even this length of time will
} leach proteins.
}
} I asked about your use or if you use a digital ccd camera on your TEM
} because the ccd will provide incredible contrast. I used to use a
} glutaraldehyde/osmium/tannic acid/osmium fixative for contrast and
} penetration. With the digital camera, I don't need the extra contrast.
}
} Regards,
} Ken
} _______________________________________
} Kenneth L. Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N Willamette Blvd.
} Portland, OR 97203 USA
}
} Tel.: 503.943.8861
} Email: tiekotte-at-up.edu
}
}
} On 9/7/05 11:17 AM, "Sue.Tyler-at-noaa.gov" {Sue.Tyler-at-noaa.gov} wrote:
}
}
}
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

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From: cgarber-at-2spi.com
Date: Fri, 9 Sep 2005 17:04:28 -0500
Subject: [Microscopy] Shelf life of silver paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Phay Fang Gan wrote:
===============================================
Question: May I know the shelf life of the conductive silver paint
and carbon paint ?
===============================================
This seemingly simple question has a complicated answer.

First, despite "conventional wisdom", the silver paint used in EM labs is
not "all the same". In addition to the obvious difference in silver solids
between products, and variations in silver colloid size, some paints
(including the SPI Supplies brands of silver paints) contain a small amount
of an "amyloid" polymer, not enough to affect negatively its conductivity,
but enough to greatly enhance its adhesive characteristics.

But this is not the only function of the presence of the amyloid polymer:
If one should forget to screw on the cap to their silver paint bottle, the
addition of the recommended thinner and a few minutes in a laboratory
ultrasonic shaker will quickly "rejuvenated" it and bring it back to life.
But those silver paints without the amyloid polymer or perhaps some other
polymer that is not so readily dissolved will either be rejuvenated much
more slowly or as we have seen, in some cases, not at all.

So if you are using at least certain silver paints, since the life time of
the silver colloid is essentially infinite, and solvent that evaporates can
be replaced with the right thinner (even to the point of its having dried
out into a brick), there is no real lifetime limit. There are legal and
other reasons why manufacturers might publish some "expiration" date for
such products but from a practical stand point, at least for some brands of
silver paint, the life time is essentially infinite.

But if your question had to do more with the lifetime of the silver paint
product unopened, and sitting on the shelf, then this has more to do with
the closure system, including the heat seal. Again, not all closure systems
are the same. I have seen some silver paint products on the shelf of
certain distributors in foreign countries where the paint was as it was
delivered ten or more years prior. And I have also seen paints of other
brands that had dried out into bricks after only a few years on the shelf.

When discussing the shelf lives of carbon paints, you could almost
substitute "carbon" for "silver" above (except that for those carbon paints
that do contain a polymer, it is not (to my knowledge an amyloid type). The
shelf life of at
least some carbon paints is indeed just as infinite as their silver paint
counterparts.

Rather than commenting further on where the SPI Supplies brand family of
silver and carbon paints fits into this picture, I would refer anyone
interested to our
silver and carbon paints "main page" at URL
www.2spi.com/catalog/spec_prep/cond_paints.html
and then draw your own conclusions.

Disclaimer: SPI Supplies is a major supplier worldwide of the SPI Supplies®
and Dotite® brands of silver paint and SPI-Chem brand of carbon paint
products used in electron microscopy and surface analysis
applications.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




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From: BFABER-at-lsc.org
Date: Fri, 9 Sep 2005 18:12:15 -0500
Subject: [Microscopy] SEM for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Liberty Science Center in Liberty State Park, Jersey City, NJ, has a lightly used 1982 Zeiss 940A SEM available immediately. This scope was serviced yearly until 2000 and used little after that up to about 6 months ago. Both vacuum pumps and chiller are working but the SEM needs some work to get operational again.

Anyone interested in this microscope, please contact Betty Faber, bfaber-at-lsc.org.



Betty Faber, Ph.D.
Leader Program Development
Learning and Teaching
Liberty Science Center



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From: r-holdford-at-ti.com
Date: Fri, 9 Sep 2005 18:15:01 -0500
Subject: [Microscopy] Re: viaWWW: shelf life of the conductive paint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I can comment on the carbon paint. I use a bottle until it's totally
gone and I can't get any more carbon in solution using isopropanol. I
should say I use SPI's conductive carbon paint because it cleans up
easily and can use isopropanol as the diluent (even though Dr. Garber
would recommend I use their thinner instead). I've been using my
current bottle for about 5 years now for SEM and FIB work. When it gets
to the consistency of chocolate pudding, I add a couple of mls of
alcohol and shake for around 5-10 minutes. As others have recommended,
definitely keep the lid on tight and shake well before and after use.

pgan-at-ap.ansell.com wrote:

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From: walck-at-southbaytech.com
Date: Fri, 9 Sep 2005 23:21:48 -0500
Subject: [Microscopy] re: temperature of 100 kV beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The temperature in the sample due to the energy being deposited in it is very dependent on the thickness of the sample. At 120 keV, if I did not deposit a sufficient layer of carbon on glass cross sections, the glass would soften under the beam. 100 keV would be worse. When I used a 200 keV machine, the problem essentially went away. For 100 keV, to avoid the problem, the illuminated area must be very thin.

One of the other things that I did that seemed to help with glass samples was to use a piece of Si as the mate to the cross section in the stack. The Si seems to take more of the heat away from the sample. Either that or it supplied a temperature insensitive portion of the total sample to prevent the sagging.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: +1-949-492-2600
FAX: +1-949-492-1499
walck-at-southbaytech.com

-------- Original Message --------
} From: bozzola-at-siu.edu
} Sent: Friday, September 09, 2005 2:54 PM
} To: Walck-at-SouthBayTech.com
} Subject: [Microscopy] temperature of 100 kV beam
}
} ----------------------------------------------------------------------------
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}
} A colleague, who is experiencing specimen damage in the TEM, inquired
} if anyone knew the temperature generated on the specimen by the
} electron beam. I realize that there are a lot of variables here, but
} even a range of temperatures would be useful.
}
} Here are his operating parameters:
}
}
} kV = 100
} spot size = 2 micrometer (specimen was examined at approximately 2
} micrometer spot size)
} specimen = ceramic containing metal particles (Ti)
} magnification = 100K to 600K
} electron gun = LaB6
} vacuum = turbo pumped to 10-5 Pa
} substrate that specimen was mounted on = Formvar/carbon coated grid
}
}
} --
} ##############################################################
} John J. Bozzola, Ph.D., Director
} I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Email: bozzola-at-siu.edu
} ##############################################################
}
} ==============================Original Headers==============================
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From: axelsson-at-acc.umu.se
Date: Sat, 10 Sep 2005 08:23:58 -0500
Subject: [Microscopy] Re: temperature of 100 kV beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't have the answer to this question but when I was renovating my
TEM I was playing around with a sample of actinolite asbestos. When we
increased the power of the beam we easily melted the fibres.
Theese were thick fibres, don't think any of them were electron
transparent so the maximum amount of energy was absorbed by the specimen.
If my memory doesn't fail me we used 100kV and no apertures. As the TEM
wasn't fully operational I have no idea of the size of the fibres.

Göran, electron microscopist wannabe

bozzola-at-siu.edu wrote:

} A colleague, who is experiencing specimen damage in the TEM, inquired
} if anyone knew the temperature generated on the specimen by the
} electron beam. I realize that there are a lot of variables here, but
} even a range of temperatures would be useful.
}
} Here are his operating parameters:
}
}
} kV = 100
} spot size = 2 micrometer (specimen was examined at approximately 2
} micrometer spot size)
} specimen = ceramic containing metal particles (Ti)
} magnification = 100K to 600K
} electron gun = LaB6
} vacuum = turbo pumped to 10-5 Pa
} substrate that specimen was mounted on = Formvar/carbon coated grid
}
}
}
}


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From: brandon-at-earthlab.net
Date: Sun, 11 Sep 2005 13:46:25 -0500
Subject: [Microscopy] viaWWW: Electron Microscopy Article Questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (brandon-at-earthlab.net) from http://microscopy.org/MicroscopyListserver/MLFormMail.html on Sunday, September 11, 2005 at 12:32:37
---------------------------------------------------------------------------

Email: brandon-at-earthlab.net
Name: Brandon Keim

Organization: Columbia University Graduate School of Journalism

Title-Subject: [Filtered] MListserver: Electron Microscopy Article Questions

Question: Dear All,

I am a freelance journalist and graduate student at the Columbia Journalism School, with a concentration in science and health writing. For a class assignment I am writing about the present state and history of electron microscopy.

If possible, I'd like to talk briefly with some of you about what electron microscopy has made possible, how it has evolved and will continue to evolve, and what you consider important to know. If anyone is interested, please feel free to get in touch; my deadline, however, is Tuesday, so the next day or two would be best.

Sincerely,

Brandon Keim

Freelance Writer
Columbia School of Journalism
c: 617 233 5346 e: brandon-at-earthlab.net



---------------------------------------------------------------------------

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From: nikola.juhasz-at-arkemagroup.com
Date: Sun, 11 Sep 2005 13:49:51 -0500
Subject: [Microscopy] viaWWW: Career Opportunity:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to post the following Career Opportunity:

SENIOR RESEARCH CHEMIST (Microscopy / X-ray)

Arkema is a $6.5-billion global chemical company with 90 production facilities worldwide and 6 research centers in the US, France, and Japan. Arkema Inc. (formerly Atofina Chemicals, Inc.) is a global producer of high-performance chemicals and polymers. Thus, we can offer many different career pathways in a dynamic, international environment. We offer a competitive salary, flexible hours, and comprehensive benefits


RESPONSIBILITIES:
Support Arkema's innovation and growth activities by providing micro- and nanoscale materials imaging and characterization support to research groups, including bulk and surface morphology, structure, mechanical properties, and composition, with particular emphasis on analysis of polymer materials. Apply sample preparation and analytical techniques that may include but are not limited to microtomy, optical microscopy, AFM, SEM with EDX, and XRF. Duties include maintaining accurate records and computer databases, and compiling, analyzing and reporting test results.


REQUIREMENTS:
Bachelors/ Masters Degree in Materials Science, Chemistry, Chemical Engineering or related discipline, with 5+ years of industrial experience. Strong emphasis in problem solving, with materials analysis, microscopy, X-ray and/or surface science experience a significant plus. The selected individual will be a self-starter, will have exceptional time and project management skills, and will have demonstrated multitasking capabilities. This position requires frequent interaction with internal customers and occasionally with external customers, which demands excellent interpersonal as well as written and oral communication skills.

Please submit your resume to:

Email: michaelp.smith-at-arkemagroup.com
Fax 610-878-6274

Arkema Inc.
900 First Ave.
King of Prussia, PA 19406
Attn: Mike Smith, Human Resources
EOE M/F/D/V

For more information regarding Arkema, please visit our website: www.arkemagroup.com.




---------------------------------------------------------------------------------------------------------
Nikola M. Juhasz, Ph.D.
Manager, Systems and Materials Analysis
Analytical and Systems Research
Arkema Inc.
900 First Avenue
King of Prussia, PA 19406
(610) 878-6408
(610) 878-6196 fax
nikola.juhasz-at-arkemagroup.com
---------------------------------------------------------------------------------------------------------

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From: richard.beanland-at-bookham.com
Date: Mon, 12 Sep 2005 03:29:08 -0500
Subject: [Microscopy] temperature of 100 kV beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I think Bill Tivol's outline of specimen heating is fine, and a very worthwhile exercise. One of the consequences of the T^4 power for radiated heat is that you don't get much heat loss by radiation below about 200C (lots of hand waving and caveats here, this is a very rough number). However I'd like to add to the emphasis on the importance of a good heat sink. I know from experience that I can fry a lift-out FIB section of InP on a holey carbon grid in a 120 kV TEM (melting point 1060C, but starts to decompose around 550C). Not very enjoyable if you just spent a few hundred £ getting the damn thing made. On the other hand I never have any problems with conventionally ion milled specimens, which have 20um thick InP on a Cu grid on the outside, tapering to the hole in the middle, and even materials like PbSn solders (melting point 183C) and Au/Ge multilayers (interdiffusion {100C) are fine if there is a good thermal path to the support grid.
From your description of the sample I guess it's a lift-out FIB section. As others have pointed out, higher kV will help since the beam-specimen interaction is less. Or you'll have no problems with a H-bar section, which has a massive heat sink all around the thin area (but you won't be able to do meaningful X-ray analysis). Or you may have to go back to the old ways of making specimens..

Good luck

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: 09 September 2005 19:53
To: Richard Beanland

A colleague, who is experiencing specimen damage in the TEM, inquired
if anyone knew the temperature generated on the specimen by the
electron beam. I realize that there are a lot of variables here, but
even a range of temperatures would be useful.

Here are his operating parameters:


kV = 100
spot size = 2 micrometer (specimen was examined at approximately 2
micrometer spot size)
specimen = ceramic containing metal particles (Ti)
magnification = 100K to 600K
electron gun = LaB6
vacuum = turbo pumped to 10-5 Pa
substrate that specimen was mounted on = Formvar/carbon coated grid


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################

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From: pcosta33-at-hotmail.com
Date: Mon, 12 Sep 2005 07:42:46 -0500
Subject: [Microscopy] viaWWW: EDX analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pcosta33-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 12, 2005 at 03:30:26
---------------------------------------------------------------------------

Email: pcosta33-at-hotmail.com
Name: Pedro

Organization: University of Cambridge

Title-Subject: [Filtered] MListserver: EDX analysis

Question: I have recently carried out some EDX analysis on a Ga-Au alloy for which I would like to a have rough idea of composition. The analysis was made for an object which is about 40 nm thick with a STEM probe in a FEG 200 kV instrument.
Since the Au K signals come at too high energy for our detector, my question is if it is possible to compare the K-shell band of Ga with the L-shell band of Au. Or would it be more correct to do that analysis comparing the two L-shell bands of Ga and Au?


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From: richard.beanland-at-bookham.com
Date: Mon, 12 Sep 2005 08:09:19 -0500
Subject: [Microscopy] RE: viaWWW: EDX analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Since all the Au-Ga compounds are known, all you need is a rough value of composition to say which one it is. I usually take a diffraction pattern or two and compare measured d-values with the international crystallographic database if there's any uncertainty. You may have to do low angle convergent beam (using a tiny condenser aperture) rather than selected area diffraction if the grains are small in a multi-phase compound. The nice thing about TEM is that you can get EDX and diffraction analysis from the same grain.
As for the EDX analysis, using different lines (K,L,M..) shouldn't be a problem anyway - if you had to do a proper job, you would be comparing it with a known standard and you can use whichever lines you like as long as there's no strong overlaps (I'm happy to be corrected on this by people who do this every day, I'm no expert)..

All the best

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pcosta33-at-hotmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 12, 2005 at 03:30:26
---------------------------------------------------------------------------

Email: pcosta33-at-hotmail.com
Name: Pedro

Organization: University of Cambridge

Title-Subject: [Filtered] MListserver: EDX analysis

Question: I have recently carried out some EDX analysis on a Ga-Au alloy for which I would like to a have rough idea of composition. The analysis was made for an object which is about 40 nm thick with a STEM probe in a FEG 200 kV instrument.
Since the Au K signals come at too high energy for our detector, my question is if it is possible to compare the K-shell band of Ga with the L-shell band of Au. Or would it be more correct to do that analysis comparing the two L-shell bands of Ga and Au?



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15, 29 -- From richard.beanland-at-bookham.com Mon Sep 12 08:09:19 2005
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From: eggert-at-mikroanalytik.de
Date: Mon, 12 Sep 2005 08:59:01 -0500
Subject: [Microscopy] Re: viaWWW: EDX analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Pedro,

you should use Ga-Ka and Au-La because of the comparable excitation and
absorption conditions with energy of 9..10 keV. This is the best choice,
even if your detector would be able to detect Au-K. But take into mind
for (only rough) concentration determinations, if the Ga/Au-
concentration ratio is expected with 1/1, then the peak-heights or
pak-net counts are like about 6/10.

Best regards

Frank

===========================
www.microanalyst.net
===========================

pcosta33-at-hotmail.com schrieb:

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From: emlabservices-at-cox.net
Date: Mon, 12 Sep 2005 09:35:44 -0500
Subject: [Microscopy] Contact Info

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I am trying to contact Steve Buckingham. Steve; are you out there?

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St.
Topeka, Kansas 66617-1780
785.246.1232 voice
785.246.0168 fax
www.emlabservices.com



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From: dmclea-at-sandia.gov
Date: Mon, 12 Sep 2005 10:14:50 -0500
Subject: [Microscopy] Tutorials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Greg,

I really like the DVD idea...I don't do as much microscopy as I used to
and I'm getting rusty! Or maybe I have that disease, you all know the
one I mean, CRS...Can't Remember Stuff! A little help is always
welcome and DVDs would be good 'cause you could pull them out when you
had a specific question or problem. I think this is a great idea.

Dorrance

-----Original Message-----
X-from: gwe-at-ufl.edu [mailto:gwe-at-ufl.edu]
Sent: Thursday, September 08, 2005 12:05 PM
To: McLean, Dorrance

Dear MSA members and other listers,
As wrangler of the MSA video collection I am often asked if we have
a full basic course in electron microscopy available on DVD. We do not.
I can also infer from folks who purchase 20 or 30 or 40 or 50 DVDs that
they intend to teach themselves what they need to know from our recorded
tutorials. (The record purchase is 52 DVDs that was filled last
month.). In light of the fact that EM coures are disappearing at
universities around the country, the Education Committee of MSA is
considering the idea of putting together a basic course or courses on
electron microscopy and make them available on DVD. Something that
would present the technology in an organized fashion and be
comprehensive enough to put a student in a position to begin work in the
area. If such a project is undertaken, Steve Barlow and Howard Berg
have agreed to work with me on coordinating it.
First, we would like to hear from the community on whether or not
they think this is a good idea. Second we would be looking for
volunteers who might be willing to record a lesson with supporting
demonstrations and other visual representations that could flesh out the
coverage. We don't want just a talking head. We would also be looking
for folks who would be willing to share the syllabi from their courses
so that we might determine how to go about organizing such a thing.
Any and all feedback is welcome.

Regarsd to all,
Greg




--
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program Scientific Director, Electron
Microscopy P.O. Box 118525
217 Carr Hall
University of Florida
Gainesville, FL 32611
Phone: 352-392-1295
Fax: 352 846 0251



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From: paul_hazelton-at-umanitoba.ca
Date: Mon, 12 Sep 2005 15:47:08 -0500
Subject: [Microscopy] image analysis

Contents Retrieved from Microscopy Listserver Archives
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for years i've been able to do the stats on my immunogold quite easily.
unfortunately i have a project which requires dealing with odd shaped
granules and inclusions in cells, and labeling on the membranes vs not
on the membranes. i'm afraid i'm going to have to come into the
computer age here, finally.

i would appreciate the advice of the list on simple programs which will
allow me to use a stylus to draw around the perimeter of the region in
the micrograph which needs to be analysed, and then give me the total
area within the region identified. don't need any more analysis. i can
still count the black spots - the eyes haven't gone yet.

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926

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From: phillipst-at-missouri.edu
Date: Mon, 12 Sep 2005 16:01:57 -0500
Subject: [Microscopy] Re: image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You can do it with Photoshop! just trace the area and look at the
histogram for total pixels counted. used Excel to convert pixels to sq.
microns. good luck. tom

At 03:48 PM 09/12/05, you wrote:



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Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
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PhillipsT-at-missouri.edu



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From: Mike.Bode-at-soft-imaging.net
Date: Mon, 12 Sep 2005 16:31:54 -0500
Subject: [Microscopy] Re: image analysis

Contents Retrieved from Microscopy Listserver Archives
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...or you could use one of the available image processing tools to do exactly what you want. Here is a link to our website (http://www.soft-imaging.com/rd/english/433.htm, click on the "gold labelling" line on the right side), but there are other programs out there that do this.

Mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: phillipst-at-missouri.edu [mailto:phillipst-at-missouri.edu]
Sent: Monday, September 12, 2005 3:04 PM
To: Mike Bode

You can do it with Photoshop! just trace the area and look at the histogram for total pixels counted. used Excel to convert pixels to sq.
microns. good luck. tom

At 03:48 PM 09/12/05, you wrote:



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Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
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PhillipsT-at-missouri.edu



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From: Elliott-at-Arizona.edu
Date: Mon, 12 Sep 2005 17:43:35 -0500
Subject: [Microscopy] image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Image J will do it also, including some stats about the area you draw.
David


On Sep 12, 2005, at 5:04 PM, phillipst-at-missouri.edu wrote:

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} You can do it with Photoshop! just trace the area and look at the
} histogram for total pixels counted. used Excel to convert pixels to
} sq.
} microns. good luck. tom
}
} At 03:48 PM 09/12/05, you wrote:
}
}
}
} } ----------------------------------------------------------------------
} } ------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
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} } http://www.microscopy.com/MicroscopyListserver
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} }
} } for years i've been able to do the stats on my immunogold quite
} } easily.
} } unfortunately i have a project which requires dealing with odd shaped
} } granules and inclusions in cells, and labeling on the membranes vs not
} } on the membranes. i'm afraid i'm going to have to come into the
} } computer age here, finally.
} }
} } i would appreciate the advice of the list on simple programs which
} } will
} } allow me to use a stylus to draw around the perimeter of the region in
} } the micrograph which needs to be analysed, and then give me the total
} } area within the region identified. don't need any more analysis. i
} } can
} } still count the black spots - the eyes haven't gone yet.
} }
} } paul
} }
} }
} }
} } Paul R. Hazelton, PhD
} } Electron Microscope Unit
} } University of Manitoba
} } Department of Medical Microbiology
} } 531 Basic Medical Sciences Building
} } 730 William Avenue
} } Winnipeg, Manitoba, Canada, R3E 0W3
} } e-mail: paul_hazelton-at-umanitoba.ca
} } Phone:204-789-3313
} } Pager:204-931-9354
} } Cell:204-781-1502
} } Fax:204-789-3926
} }
} } ==============================Original
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}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 2 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
}
}
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From: thoward-at-unm.edu
Date: Mon, 12 Sep 2005 17:50:17 -0500
Subject: [Microscopy] help from a polymer TEM person?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One of our TEM service reps told me that he's been told that many people
who looks at polymers in the TEM "pre-burn" their samples under a UV lamp
before putting the grids in the column - is this true? If so, how is it
done (for how long, distance from bulb, etc.)?

We have a weird "thing" with our TEM that burns a pattern into resin
sections and we were discussing ways to pre-burn; chemical stetching
doesn't help, and doing it in the column is too slow when there are a lot
of samples. If I could do a mass burn I'd be set!

Thanks!

Tamara

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|

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From: DrJohnRuss-at-aol.com
Date: Mon, 12 Sep 2005 18:16:24 -0500
Subject: [Microscopy] Re: image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 9/12/05 4:48:36 PM, paul_hazelton-at-umanitoba.ca writes:

} i would appreciate the advice of the list on simple programs which will
} allow me to use a stylus to draw around the perimeter of the region in
} the micrograph which needs to be analysed, and then give me the total
} area within the region identified. don't need any more analysis. i can
} still count the black spots - the eyes haven't gone yet.

Just about any program out there - including NIH Image which is free - will
do that. But why in the world would you NOT want the program to do the
counting, too? Lots of tests have demonstrated that people don't really count things
very well. And even if you CAN do it accurately, you certainly can't do it as
quickly as the computer.


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From: phillipst-at-missouri.edu
Date: Mon, 12 Sep 2005 18:30:47 -0500
Subject: [Microscopy] image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have ImagePro and MetaMorph and the ImageTool Kit and have used them all
successfully. But I disagree with John that using a computer is always
easier and better. Maybe if I had written the book on image processing like
John Russ, I could take my TEM digital images and have the computer
automatically threshold, detect and count the colloidal gold particles
against a typical cell background in a reasonable amount of time. Despite
being modestly familiar with the morphometric software packages, I often
still find it easier and faster to count small amounts of gold particles by
eye. I use a program like Photoshop to place a colored dot on top of each
gold particle as i click it. My experience is that i can do a lot of images
fast and not have to worry about losing gold particles touching black
membranes or something that screws up my thresholding detection in a random
set of real world images. I can always tweak the thresholding for a single
image but often find the next image needs a tad more tweaking. I guess if I
was a lot better at image processing or my samples were more idealized, I
would agree with John's comment but most people aren't as good as he is so
I think there is a place for those of us who count by eye.



At 06:17 PM 09/12/05, you wrote:



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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: Dmrelion-at-aol.com
Date: Mon, 12 Sep 2005 18:51:56 -0500
Subject: [Microscopy] 100 kV beam heating effects

Contents Retrieved from Microscopy Listserver Archives
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I have followed this discussion with some interest because a similar situation prevails in cathodoluminescence (CL) instrumentation, including those used on optical microscopes (10 to 20 kV, 0-1 mA electron beam).

The beam current is obviously an important variable since the total power is the product of beam voltage and beam current. This can be as much as 10 watts or more in the CL instruments (15 kV and 0.7 mA typical operating condition). One of the questions that comes up immediately is the measurement of the beam current. I am not familiar with the methods of electron microscopy but in CL microscopy often the beam current "displayed" is the total current from the high voltage supply, usually measured in the ground return line. The actual current to the specimen can be significantly less than this because a portion of the beam is intercepted on various anode apertures, collimators, etc., depending on the particular instrument design.

It is possible to measure the current to the specimen if it is set up with a suitable Faraday cup arrangement to suppress secondary electrons but this is rarely done.

Sample temperature discussions often go back to Carslaw and Jaeger, 1959, Conduction of Heat in Solids (general solution for a point source on a semi-infinite solid) and Castaing, R. 1952 (Thesis - Application des sondes electronique a une methode d'analyse ponctuelle chimique et cristallographique.). Castaing's solution is appropriate to electron probe conditions.

With the geological thin sections that are the usual subject for optical CL, the lateral thermal conductivity of the section is often a big question mark also. Tight, well-cemented, samples have relatively high thermal conductivity. But there are examples of quartz sandstones with high porosity where the individual quartz grains in the thin section do not make contact with adjacent grains - only with the epoxy imbedding media. I have seen situations where an individual grain is so well thermally isolated by the epoxy that it would glow brightly with cathodoluminescence and then become "red hot" to the unaided eye while adjacent grains would appear undisturbed..

Don Marshall

Donald J. Marshall (Dr.)
RELION Industries
PO Box 12
Bedford, MA 01730
USA

781-275-4695 (phone)
781-271-0252 (FAX)

dmrelion-at-aol.com

{http://www.excitingelectrons.com/} http://www.excitingelectrons.com

"A weed is a flower out of place."

message ends



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From: keith.morris-at-ucl.ac.uk
Date: Tue, 13 Sep 2005 04:21:34 -0500
Subject: [Microscopy] image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

With regard to the image analysis problem in hand (TEM and 'black' gold
particles), ImageJ (or NIHImage in its Apple variant) is probably the best
way to go as it is a powerful image analysis program and free to use. It is
a little complicated in its user interface though (not that MetaMorph is
actually much better in this respect for the extra £3000). For a stylus
input (cell tracing) and a photo holding pad you need to get one of those
Wacom Graphire pads for £80 or so (www.wacom.com). I have to say I and many
others never got on with the Wacom stylus/pad I bought, but other users in
the department seem to love them. I am happier with the mouse. The Graphire
tablets work on the Apple and PC, integrate into programs (and also have a
mouse).

Find ImageJ (PC) at http://rsb.info.nih.gov/ij/ and NIH Image (Apple) at
http://rsb.info.nih.gov/nih-image/. As mentioned PhotoShop can trace round
images to give pixel area - but ImageJ is a proper image analysis program
and would be useful in other projects. Electron micrographs are often tricky
to threshold , so tracing is probably quicker (although try size parameters
to remove the larger detected objects, and total area / mean individual
object area). When using ImageJ have a good look at all the plug-ins (I
download and install any that seem even vaguely interesting). The basic
package can do the area measurements and grain counts once calibrated
(Analyze, Set Scale) and thresholded. ImageJ also has a selection of image
processing commands (but a duff image remains a duff image afterwards).

If you have a had a bit of luck on the gee gee's, try Image Pro whose home
is http://www.mediacy.com/ and MetaMorph at
http://www.universal-imaging.com/. These programs are pretty slick for most
applications, and have lots of extra's like cell motility tracking, but
MetaMorph in particular is idiosyncratic and difficult to get to grips with
for the casual user (but its mostly all in there somewhere). Useful image
processing applications like deconvolution are extra (a lot extra). They are
both expensive basic packages as well. OpenLabs at
http://www.improvision.com is also OK for this sort of thing, and is Apple
based, but its better at image capture and time-lapse as its image analysis
component is relatively poor (although their Velocity package is a great 3D
reconstruction program at £10,000).

I have been using image analysers since the 1970's Quantimet 720 and I have
to say that generally it is often easier to count by eye rather than use the
image analyser. This is particularly true for things I count like fibres
(complex counting rules) and alpha track stars (that have tracks that vary
in number, length and thickness). However if you have hundreds of very
bright or very black dots on the image that can be distinguished easily by
thresholding then obviously the image analysis program can count them
easily. Plus if you are counting hundreds of grains within a sample if
doesn't really matter if the image analyser counts 950 and you count 980 -
both counts are probably well within biological variation. Just pick the
quickest way to count (often by the time you have processed an image for
automatic counting often you could have counted two images by eye,
particularly if there aren't that many objects). Modern programs should have
a manual count option anyway - click on the screen and the object is ticked
off, numbered and counted. Also do make yourself a help file document of how
you did something with an image analysis package, as its often difficult to
remember how you did it a few months on (PrtSC to copy and paste the program
menu VDU view is useful).

Older dedicated hardware based image analysers like the UK Magiscan Colour
and the Seescan systems were often faster than modern multi-tasking PC
versions and included things like real-time binary image editors and light
pens (and they never seemed to crash). They also seemed to be more tuned
into scientific research needs (e.g. their separate detected object
algorithms worked far better in 1989 than those in Metamorph, ImagePro or
OpenLabs do now). With any image analysis its the quality of the sample and
captured image that is paramount, so its worth spending time on sample
preparation and setting up the microscope (e.g. for DIC). Uneven
'illumination' and a poor specimen is a disaster in light microscopy (and
EM) image analysis, although background correction can help in light
microscopy. Trying to recover information from a poor specimen by image
analysis techniques is not the way to go.

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


----- Original Message -----
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To: {keith.morris-at-ucl.ac.uk}
Sent: Tuesday, September 13, 2005 12:35 AM


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From: famos-at-ufl.edu
Date: Tue, 13 Sep 2005 08:08:43 -0500
Subject: [Microscopy] T

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--
FAIRLAND FONTILLAS AMOS, PhD
Materials Science and Engineering
University of Florida


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From: famos-at-ufl.edu
Date: Tue, 13 Sep 2005 09:16:07 -0500
Subject: [Microscopy] TEM intensity of spots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am working with calcium carbonate and I am trying to index a
single crystal film. Should the relative intensities reported in
the JCPDS match the relative intensities in the selected area
diffraction pattern.

--
FAIRLAND FONTILLAS AMOS, PhD
Materials Science and Engineering
University of Florida


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From: rjharris-at-uwo.ca
Date: Tue, 13 Sep 2005 09:17:47 -0500
Subject: [Microscopy] image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



-----Original Message-----
X-from: Richard Harris [mailto:rjharris-at-uwo.ca]
Sent: Tuesday, September 13, 2005 10:12 AM
To: paul_hazelton-at-umanitoba.ca

for years i've been able to do the stats on my immunogold quite easily.
unfortunately i have a project which requires dealing with odd shaped
granules and inclusions in cells, and labeling on the membranes vs not
on the membranes. i'm afraid i'm going to have to come into the
computer age here, finally.

i would appreciate the advice of the list on simple programs which will
allow me to use a stylus to draw around the perimeter of the region in
the micrograph which needs to be analysed, and then give me the total
area within the region identified. don't need any more analysis. i can
still count the black spots - the eyes haven't gone yet.

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926

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From: bci-at-cypress.com
Date: Mon, 12 Sep 2005 15:55:49 -0500
Subject: [Microscopy] image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have had success with a free image processing program known as UTHSCSA
Image Tool (link below). I have used it in the past to bin and tag
varying sizes of pores in ceramic films.

http://ddsdx.uthscsa.edu/dig/itdesc.html

cheers,
cj


CJ Bonifas
Engineer, Failure Analysis Group
Cypress Semiconductor (Minnesota), Inc.
2401 East 86th Street
Bloomington, MN, USA 55318

bci-at-cypress.com
952.851.5370






-------- Original Message --------

for years i've been able to do the stats on my immunogold quite easily.
unfortunately i have a project which requires dealing with odd shaped
granules and inclusions in cells, and labeling on the membranes vs not
on the membranes. i'm afraid i'm going to have to come into the
computer age here, finally.

i would appreciate the advice of the list on simple programs which will
allow me to use a stylus to draw around the perimeter of the region in
the micrograph which needs to be analysed, and then give me the total
area within the region identified. don't need any more analysis. i can
still count the black spots - the eyes haven't gone yet.

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926

==============================Original Headers==============================
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From: rcsaic-at-sbcglobal.net
Date: Tue, 13 Sep 2005 09:35:24 -0500
Subject: [Microscopy] TEM intensity of spots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

No generally not, there are dynamical diffraction effects in electron
diffraction that affect the intensity of spots. This includes double
diffraction effects that can allow some classes of forbidden reflections
(those forbidden by glide planes and screw axis) to occur. There are
innumerable other factors that make the intensities different as well.

Use the d-spacings and forget the intensities.

In order to index electron diffraction patterns it really help to have a
complete list of all d-spacings and corresponding symmetrically-equivalent
hkls for a given material. Such a list needs to be calculated from the cell
parameters using appropriate software. I use some home-grown software to do
this. I don't know if there is any commercially available software on the
market right now that will do it. (Listserver folks help if you know...)

Calcium carbonate has the R3barC space group and will have dynamically
allowed and dynamically forbidden reflections depending on whether you
calculate the d-spacing based on the primitive rhomobohedral or center
hexagonal cell.

Hope this helps.

Roy Christoffersen
SAIC
NASA Johnson Space Center

-----Original Message-----
X-from: famos-at-ufl.edu [mailto:famos-at-ufl.edu]
Sent: Tuesday, September 13, 2005 8:21 AM
To: rcsaic-at-sbcglobal.net

I am working with calcium carbonate and I am trying to index a
single crystal film. Should the relative intensities reported in
the JCPDS match the relative intensities in the selected area
diffraction pattern.

--
FAIRLAND FONTILLAS AMOS, PhD
Materials Science and Engineering
University of Florida


==============================Original Headers==============================
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From: gary.m.brown-at-exxonmobil.com
Date: Tue, 13 Sep 2005 09:49:49 -0500
Subject: [Microscopy] Re: help from a polymer TEM person?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

"The statements and opinions expressed here by Gary M. Brown represent
neither those of ExxonMobil Corporation nor its affiliates."

Tamara,

Ultrathin sections of oriented polymers (stained or unstained) often deform
when first exposed to the beam. This relaxation can be achieved prior to
analysis by low-dose exposure to the electron beam for several minutes at
low magnification. The objective is to relax the sections and make them
physically stable during microscopy. I prefer not to use this procedure
because it can cause significant deformation of the sections.

A better procedure, in my opinion, is to mount the sections on high quality
continuous carbon film grids. Do not use Formvar or Formvar/carbon films
since Formvar films are not very clean and can cause problems during
imaging and elemental analysis. The sections adhere to the carbon film and
will not deform under the beam, thus eliminating artifacts relaxation
and/or orientation in images. Image quality is still very good. One must
still be careful about beam damage, since this is still a real possibility,
carbon film or not.

Hope this helps,

Gary M. Brown
ExxonMobil Chemical Company
Baytown Technology & Engineering - West
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: 281 834 2387
fax: 281 834 2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



thoward-at-unm.ed
u
To
gary.m.brown-at-exxonmobil.com
09/12/05 05:51 cc
PM
Subject
[Microscopy] help from a polymer TEM
Please respond person?
to
thoward-at-unm.ed
u










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One of our TEM service reps told me that he's been told that many people
who looks at polymers in the TEM "pre-burn" their samples under a UV lamp
before putting the grids in the column - is this true? If so, how is it
done (for how long, distance from bulb, etc.)?

We have a weird "thing" with our TEM that burns a pattern into resin
sections and we were discussing ways to pre-burn; chemical stetching
doesn't help, and doing it in the column is too slow when there are a lot
of samples. If I could do a mass burn I'd be set!

Thanks!

Tamara

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|

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From: Mike.Bode-at-soft-imaging.net
Date: Tue, 13 Sep 2005 10:06:49 -0500
Subject: [Microscopy] RE: image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Paul,

There is a new add-in for the analySIS software that does pretty much exactly what you want. It identifies the gold particles not only by their grey level, but also by their "roundness" and expected diameters. You can also analyze double-labeled samples, etc. I don't want to make this too commercial here, so please contact me via email. I am also looking for beta-testers of the software. If you (or anybody else) is interested, please contact me.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================




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Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: gala-instrumente-at-t-online.de
Date: Tue, 13 Sep 2005 15:40:37 -0500
Subject: [Microscopy] AW: help from a polymer TEM person?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Tamara,

I have thought about your inquiry for several hours and
waited for anybody to respond .
Gary Brown´s advice makes sense.

UV lamp produces oxygen radicals and therefore attacks
carbon compounds as a preferred chemical reaction
partner.
Using a UV-lamp is very slow and results, depend on
distance to the source and radiant heat.

Nevertheless, I recommend a plasma treatment before
analysis.
This could possibly mean cleaning, surface modification
and conditioning in one step.
Hopefully, you do have a plasma instrument in your lab
to test.
Try air or bottled oxygen for a start.

Kind Regards
Jost

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
GaLa Gabler Labor Instrumente Handels GmbH
An der Schmalmach 42
D - 65307 Bad Schwalbach
Germany
Tel: +49-6124-77 952
Fax: +49-6124-60 274
gala-instrumente-at-t-online.de
http://www.gala-instrumente.de/
http://www.plasmainstrument.com/
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

-----Ursprüngliche Nachricht-----
Von: thoward-at-unm.edu [mailto:thoward-at-unm.edu]
Gesendet: Dienstag, 13. September 2005 00:54
An: gala-instrumente-at-t-online.de
Betreff: [Microscopy] help from a polymer TEM person?



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Society of America

One of our TEM service reps told me that he's been told
that many people
who looks at polymers in the TEM "pre-burn" their
samples under a UV lamp
before putting the grids in the column - is this true?
If so, how is it
done (for how long, distance from bulb, etc.)?

We have a weird "thing" with our TEM that burns a
pattern into resin
sections and we were discussing ways to pre-burn;
chemical stetching
doesn't help, and doing it in the column is too slow
when there are a lot
of samples. If I could do a mass burn I'd be set!

Thanks!

Tamara

|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward-at-unm.edu
|--------------------------------------------------|

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From: dfine-at-seton.org
Date: Tue, 13 Sep 2005 18:46:09 -0500
Subject: [Microscopy] AskAMicroscopist: procedure for liver biopsies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dfine-at-seton.org) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, September 13, 2005 at 14:57:45
---------------------------------------------------------------------------

Email: dfine-at-seton.org
Name: Diann Fine

Organization: Brackenridge Hospital

Education: Graduate College

Location: Austin, Texas USA

Question: I work in the Electron Microscopy area of the histology department.I would appreciate a procedure for liver biopsies. I am using the new ERL-4221,Cycloaliphatic Epoxide Resin from EMS.The thick and thin sections look hazy and cloudy. I wondered if it could be the resin.Do you use it in the same proportions as the old VCD?

---------------------------------------------------------------------------

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From: pjm-at-gol.com
Date: Tue, 13 Sep 2005 23:31:45 -0500
Subject: [Microscopy] RE: relocating objects on a flipped slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Damien (and others who replied to this question),

Thanks for your kind advice.

I expect the double cover-slip procedure will be needed. However for
the repeated handling of many samples, it would be good if there was
some kind of holder system that made the coverslips easy to handle.

I will probably put the coverslips in a cardboard sandwich with holes.

If it was of interest to many people, perhaps a holder like this
could be mass-produced at low cost, with covered strips of adhesive
ready-to-go.

Best regards, Peter

*********


} Peter,
}
} I'm not sure that this is going to work very well if you are talking about a
} specimen on a regular microscope slide with a standard cover slip. One
} reason is that the slide is much thicker and you may not have sufficient
} working distance at higher magnifications unless you are using extra long
} working distance lenses. I don't know of any such ELWD oil immersion lenses
} for higher magnification work. Furthermore you may not be able to obtain
} Koehler illumination.
}
} The effect of the thicker microscope slide glass on the image is another
} issue. I would suggest that you prepare your sample between two large cover
} slips in whatever mounting media you are using and tack them together with
} either clear tape or a thin coating of clear finger nail polish, or other
} adhesive. Then you should be able to flip them over and not worry about one
} slipping away from the other and if you are using water, they will not dry
} out as quickly.
}
} Damian Neuberger, Ph.D.
} Consultant
} Microscopy/Digital Imaging/Image Analysis
} 2416 Covert Rd
} Glenview IL 60025
} Tel: (847) 998-8574
} email: neuberger1234-at-comcast.net {mailto:neuberger1234-at-comcast.net}

--
Peter Matthews (Dr)
National Museum of Ethnology
Senri Expo Park, Suita City
Osaka 565-8511, Japan
Tel. +81 6 6876-2151 (museum)
Tel. +81 6 6876-8357 (Peter's office)
Fax +81 6 6878-7503 (museum)

Websites:

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A meeting place for research writers, editors, translators and proofreaders


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From: weill-at-icmcb-bordeaux.cnrs.fr
Date: Wed, 14 Sep 2005 02:03:53 -0500
Subject: [Microscopy] Re: TEM intensity of spots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello

The intensity in electron diffraction are subject tro many parameters such
as doubble diffraction , thisckness of the specimen. So they very often do
not match those reported in the JCPDS data base.
To indexe you should only consider the position of the reflexions.
A programm like "CaRine Crystallography" may help you to do that



A 16:21 13/09/2005, vous avez écrit :



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WEILL Francois

{http://www.icmcb.u-bordeaux.fr/} ICMCB
Avenue du Dr A. Schweitzer
33608 Pessac cedex
France

tel : +33 (0)5 40 00 26 54
fax : +33 (0)5 40 00 27 61
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From: david.morgan-at-christ-church.oxford.ac.uk
Date: Wed, 14 Sep 2005 07:06:39 -0500
Subject: [Microscopy] SEM: Digital Camera for Hitachi S-800

Contents Retrieved from Microscopy Listserver Archives
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Hello Everyone,

We're thinking of replacing the polaroid model 545 4x5 film holder in =
our Hitachi S-800 SEM with a camera that will allow us to take digital =
images instead. Does anyone have any suggestions for models that will =
fit our microscope?

Thanks,

David Morgan
Department of Chemistry
University of Oxford

==============================Original Headers==============================
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From: yschwab-at-titus.u-strasbg.fr
Date: Wed, 14 Sep 2005 07:41:37 -0500
Subject: [Microscopy] RE: image analysis

Contents Retrieved from Microscopy Listserver Archives
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If you don't use digital images, you can also use a transparent paper
that you place on top of your micrograph. You draw the area you're
interested in, you cut it out with cisors and you weight it with a
precision scale. After determination of your weight-to-surface factor
(just weight a 1 cm2 piece of the same paper), you don't need any
computer to determine the surface. It's easy and acurate (and fast).

cheers

Y

--
_________________________________________________________

Yannick Schwab
Service de Microscopie Electronique
IGBMC
1, rue Laurent Fries
67404 Illkirch Cedex
France
Tel +33(3) 88 65 56 06
Fax +33(3) 88 65 32 01
yschwab-at-igbmc.u-strasbg.fr
www-igbmc.u-strasbg.fr/MIF/mif.html
_________________________________________________________




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From: donoleary-at-att.net
Date: Wed, 14 Sep 2005 08:50:18 -0500
Subject: [Microscopy] LM, Workshop on use of the microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Peter,

Re "adhesive strips". Somewhere in the deep dark recesses of my memory, I
recall that someone manufactured something that would form a strip of
adhesive on a slide onto which a cover slip would be laid. Sort of a
transfer system. I think it had to do with cell culture work and created a
tiny chamber.

Damian

-----Original Message-----
X-from: Peter Matthews [mailto:pjm-at-gol.com]
Sent: Tuesday, September 13, 2005 11:32 PM
To: Damian Neuberger
Cc: Microscopy Listserver

New York Microscopical Society
Bernard Friedman Memorial Workshops
Use of the Microscope
October 1, 8, 15,22 2005
A basic course on light microscopy which will cover the following topics:

Theory of microscopy, Kohler Illumination
Diffraction Theory, Contrast Methods
Polarized light, Phase Contrast, Interference
Hoffman contrast, Rheinberg, Dark-field & oblique Illumination, etc.

The workshop will consist of four consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of microscopy. The course instructors are Jan Hinsch formerly of Leica Microsystems, Inc., Dennis O’Leary of Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of Smiths Detection, Inc. and N.Y.M.S. Instructor Don O'Leary.

WHEN: October 1, 8, 15,22 2005 from 10 A.M. to 4 P.M.

WHERE: 30 N. Mountain Avenue, Montclair, NJ, Phone (973) 744-0043 (parking available, accessible by public transportation. Information on car pools and transportation will be provided.)

COST: $395 for NYMS members, $425 for non-members (includes membership) Lunch and course materials are included. Checks made out to NYMS.

WHO: Beginners and experienced users who wish to learn more about the proper use of a microscope.

HOW: Register using form below. Limited to the first 12 registrants.
Send form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849
E-mail: donoleary-at-worldnet.att.net Fax (425) 988-1415

PLEASE POST

-------------------------------------------------------------------------
Registration Form Use of the Microscope 2005

N.Y.M.S. Member_________________ ($395) Non-Member__________($425)

Name______________________________________________________________________
Address____________________________________________________________________
Phone (W)_______________________(H)___________________________

e-mail address____________________________

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From: eschumacher-at-mccrone.com
Date: Wed, 14 Sep 2005 08:52:30 -0500
Subject: [Microscopy] Short Course Announcement: Scanning Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Fellow Listers,

The College of Microscopy will be offering a short course, Scanning
Electron
Microscopy, October 17-21, 2005, at our Westmont Facility. In addition
to lectures,
the course emphasizes hands-on training using five scanning electron
microscopes and
electron microprobe analyzers, and gives students the opportunity to
work on their
own samples. For further details and registration information, please
follow the
link below.

www.collegeofmicroscopy.com

Elaine Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com



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From: brakenho-at-science.uva.nl
Date: Wed, 14 Sep 2005 09:11:53 -0500
Subject: [Microscopy] Focus on Microscopy FOM 2006, Perth, Australia, April 9-12, 2006

Contents Retrieved from Microscopy Listserver Archives
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FOCUS ON MICROSCOPY 2006, Perth, Australia, April 9-12, 2006
19th International Conference on 3D Image Processing in Microscopy
18th International Conference on Confocal Microscopy

Dear Colleagues

After the successful FOM2005 conference held in Jena in March this year,
it is a pleasure to announce that the next conference: Focus on Microscopy
2006 will take place in Perth, Western Australia, April 9-12, 2006. As
the next in a series of unique interdisciplinary meetings on advanced
multidimensional light microscopy and image processing, the conference
will be hosted by the University of Western Australia in Perth. The
conference will be located at the beautiful Esplanade Hotel in the
historic waterfront suburb of Perth, Fremantle.
Focus on Microscopy 2006 is the continuation of a successful conference
series presenting the latest innovations in optical microscopy and its
applications in biology, medicine, material science, and information
storage. 3D optical imaging and related theory are important subjects for
the conference. The series is as relevant now as at any time in its
history as the scientific and engineering communities strive to meet the
needs of a surging life sciences sector, as well as respond to the
sustained pressure for miniaturization in lithography and data storage.
The conference series is known for covering the rapid development of
advanced fluorescence labelling techniques for the confocal and
multi-photon 3D imaging of -live- biological specimens. This year, in
addition, special attention will be given to imaging in thick tissues and
the use of laser light as an active tool at sub-micrometer length scales
for cell biology, nanobiotechnology, and medicine.

Abstracts for contributions are invited and can already be submitted
through the website:
www.FocusOnMicroscopy.org
where further information on the present and previous FOM conferences can
be found.

Important dates:
Deadline for the submission of abstracts: January
9, 2006
Acceptance of contributions, draft program:
January 23, 2006
Deadline for early registration: February 20, 2006

We would be very pleased to welcome you to Perth for the FOM2006
conference and exhibition.

On behalf of the organising committee,

David Sampson,
University of Western Australia, Perth, Australia

Fred Brakenhoff
Swammerdam Institute for Life Sciences, University of Amsterdam, The
Netherlands

E-mail: info2006-at-FocusOnMicroscopy.org

Web: www.FocusOnMicroscopy.org



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From: james.romanow-at-uconn.edu
Date: Wed, 14 Sep 2005 09:27:10 -0500
Subject: [Microscopy] Job Opportunity-TEM Life Science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The following message is from a former colleague of mine at UConn.

Jim Romanow
The University of Connecticut
Electron Microscopy Laboratory
Physiology and Neurobiology Department
Bio-Physics Building, Room G06, Unit 3242
91 North Eagleville Road
Storrs, CT 06269-3242
860 486-2914
james.romanow-at-uconn.edu

----------------------------------------------------------------------------
---------------------------------------

Dear Jim,
I thought that I would forward the job description to you, on the
off-chance that you might know someone who was looking for such a position.
Any interested parties can follow the link or email their Resume to me, and
I can pass it on to my boss.
Best regards,
Dave Horspool
DHorspool-at-FEICO.com

-----------------------------------------------------------
Available Job at FEI Company
-----------------------------------------------------------

Senior Applications Engineer - TEM (Life Science) Hillsboro, OR

Salary: Open
Duration: Full Time
Job ID: 112-HBO
Post Date: 06/30/2005

Description:
The primary responsibility of this position is supporting the sales force
and training customers on instrumentation and applications. Secondary
responsibilities include acting as a technical resource for sales and
service. This will manifest itself in the form of pre-sale visitations to
customer sites to explain technical details and give technical
presentations, as well as pre-sale system demonstrations either at customer
sites or applications laboratories. Service support will be given when
certain applications work has to be performed before a system is signed off.
This job also involves supporting customers after the sale has been made and
the system is signed off.

Key Responsibilities:

* Cultivating positive customer relationships
* Act as a high level customer interface for specific instrument/
applications issues
* Assisting customers in technique development
* Supporting service engineers on instrument sign offs and specific
application techniques
* Providing feedback to the product groups on system performance, features
and problems
* Providing input to marketing/development groups for future product
developments

This position is ideal for an applicant who wants to be involved with a
dynamic, customer, life science market focused team.

Education, Experience and Qualifications:

* The successful candidate will have a degree in the Biological Sciences
(Cell and Molecular Biology, Neurobiology). Higher degree is preferred

* 3 years experience in a relevant commercial/research establishment
* Excellent communications skills
* Well-developed interpersonal skills with a diverse audience
* Knowledge of electronic systems/diagrams, as well as complex vacuum
systems useful
* Diagnostic capabilities as well as faultfinding techniques will be highly
valued
* Operational experience of analytical equipment; specifically transmission
electron microscopes, various energy dispersive spectrometers and Cryo
techniques a plus

* Excellent working knowledge and confidence with PC platforms, especially
Windows 2000 as well as 3D reconstruction/ Tomography programs and Power
Point skills is highly desirable.

Suitable candidate must be willing and able to travel both domestically as
well as internationally and must possess a valid passport.

Required Skills:
FEI Company is an Equal Opportunity Employer

Follow this link to apply for this position:

http://www.careerexchange.com/cejobs/applyFEI_int.asp?fei?fei112-HBO?jjeske




Dr D.N.Horspool
Sr. Applications Engineer
FEI Company,
5350 NE Dawson Creek Drive
Hillsboro
OR 97124 USA




==============================Original Headers==============================
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From: ptomic-at-ciclonsemi.com
Date: Wed, 14 Sep 2005 09:37:31 -0500
Subject: [Microscopy] SEM: Digital Camera for Hitachi S-800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

David,

Have you considered a "screen capture" system that will take the video and
store the image? It just may be cheaper. The one caveat about this approach
is that you may need to cut into the appropriate points in the video drive
circuit since many older microscopes do not bring these points out to a
connector. My experience was with a Hitachi S570 and it was a little messy
but it did work. My need was to do EDX mapping so the video capture was an
integral part of the x-ray system.

Regards,

Peter Tomic

Ciclon Semiconductor Device Corporation



-----Original Message-----
X-from: david.morgan-at-christ-church.oxford.ac.uk
[mailto:david.morgan-at-christ-church.oxford.ac.uk]
Sent: Wednesday, September 14, 2005 8:13 AM
To: ptomic-at-ciclonsemi.com

Hello Everyone,

We're thinking of replacing the polaroid model 545 4x5 film holder in =
our Hitachi S-800 SEM with a camera that will allow us to take digital =
images instead. Does anyone have any suggestions for models that will =
fit our microscope?

Thanks,

David Morgan
Department of Chemistry
University of Oxford

==============================Original Headers==============================
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From: randerson20-at-tampabay.rr.com
Date: Wed, 14 Sep 2005 10:11:18 -0500
Subject: [Microscopy] Re: TEM intensity of spots

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

From nearly 40 years of working with electron and x-ray diffraction
patterns, plus several years of membership in the JCPDS, now ICDD, I can
offer a rule of thumb regarding intensity of ediff vs. x-ray diffraction
data: Strong reflections are strong reflections and weak are weak. One
cannot make an identification of an unknown phase using ediff ring data
where very strong x-ray lines are missing from one's pattern, without
giving crystal-chemical reasons to account for the missing reflections.
Likewise, a 5% intensity x-ray line will not suddenly become a 100%
intensity ediff ring-pattern reflection.

Electron diffraction patterns will SOMETIMES have extra and structure
factor forbidden spots (& rings, as appropriate) due to double
diffraction and relaxation of structure factor rules due to specimen
thickness effects with thin TEM specimens, etc. --Emphasis on
"sometimes."-- With regard to solving for unknown phases using ediff
data, the extra spots/rings, when present, are either a help or a
hindrance as they are most conspicuously present at large d-values,
which are the most diagnostic d-values for phase identification. In the
rare instances where I had a true unknown specimen in the TEM, and I
thought I had a match with a phase in the ICDD x-ray data base, except
for the presence of weak, large d-spacing reflections in the ediff data,
I could sometimes confirm my identification by computing forbidden
(100), (110), etc. reflections and matching them to my experimental
data. Should an image of your specimen show it to be loaded with twins
or other features that cause extra reflections, you should make
appropriate forbidden reflection calculations early-on.

The ICDD has products to aid electron diffractionists. The
Max-d/Alphabetical Index (now called the Long-d index, I think), and
products derived from the Sandia database come to mind. Check www.icdd.com.

Fairland: the answer to your question is "Yes, probably."

Ron Anderson

Disclaimer: I am an emeritus member and a fellow of the ICDD with no
direct interaction with them for several years now.


famos-at-ufl.edu wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America



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From: MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 14 Sep 2005 12:34:10 -0500
Subject: [Microscopy] New England Society for Microscopy October 12th Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Please join your fellow NESM members for their Fall 2005 meeting to be held at
Worcester Polytechnic Institute (WPI) in Worcester, MA on Wednesday, October
12th. Don't forget to invite your colleagues!

The meeting will be held in the historic Higgins House on Wing Road.
Registration for this meeting will be $25.00 which will include a reception
featuring a beer/wine bar and dinner.

Registration will be from 5:00-6:00pm, followed by a reception/dinner from
5:30-7:00pm. The technical presentations will begin at 7:00pm.

We have 3 exciting speakers scheduled: Philip Klausmeyer of the Worcester Art
Museum who will speak on applications of microscopy in art conservation; Michael
Jercinovic of the Department of Geosciences at UMASS, Amherst who will present
new techniques in x-ray microanalysis, and WPI's own Eric Overstrom from the
Department of Biology and Biotechnology who will give us insight in visualizing
the key enablers of oocyte developmental competence.

For a map and directions to Higgins House click
http://nesm.cims.harvard.edu/Misc/wpidirections.htm

For the full meeting program, together with abstracts and biosketches of the
speakers, click http://nesm.cims.harvard.edu/Misc/program.htm


PRE-REGISTRATION IS REQUIRED! Dan Gibson of WPI is Chair of this meeting and he
needs a head count for dinner. Please send a check, made out to NESM for
$25.00, by Friday, October 7th to: Paul Bain, NESM Treasurer, Countway 212, 10
Shattuck Street, Boston, MA 02115.
(email: Paul_Bain-at-hms.harvard.edu )

We hope to see you there!










Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org


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From: jfb-at-uidaho.edu
Date: Wed, 14 Sep 2005 13:11:26 -0500
Subject: [Microscopy] Looking for Jose Mascorro

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was wondering if anyone has been in touch with Jose Mascorro since the
hurricane hit New Orleans.

Franklin Bailey
University of Idaho
Moscow, Idaho


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From: SHem-at-laurentian.ca
Date: Wed, 14 Sep 2005 14:40:56 -0500
Subject: [Microscopy] Gas for WD spectrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi there,
We're setting up a vintage instrument, a Cambridge S-250 with
a vintage microspec WD spectrometer.

This spectrometer needs P10, which is to be expected, but also P90.
Praxair don't know what this is, logic dictates that its the inverse composition of P10 i.e.
90% methane 10% argon. Do anyone have a similar instrument and knowledge of this gas ?
Thanks,
Skage

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-675-1151 x4040
cell. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html



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From: MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 14 Sep 2005 15:37:20 -0500
Subject: [Microscopy] [Microsocpy] re: LCM fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What do other universities, etc. charge for using a Laser Capture
Microdissection System? We are starting to have outside users on the system.

Thanks!
Peggy


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org


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From: pmccurdy-at-lamar.colostate.edu
Date: Wed, 14 Sep 2005 18:12:36 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist:Help with upgrading Vantage 2.4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pmccurdy-at-lamar.colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, September 14, 2005 at 11:02:37
---------------------------------------------------------------------------

Email: pmccurdy-at-lamar.colostate.edu
Name: Pat McCurdy

Organization: Colorado State University

Education: Graduate College

Location: Fort Collins, Colorado, USA

Question: I am interested if anyone on this list has experience upgrading Vantage 2.4 software for Window NT on the ThermoElectron Noran EDS dector to Windows 2000 or Windows XP?

---------------------------------------------------------------------------

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7, 13 -- Subject: [Filtered] AskAMicroscopist:Help with upgrading Vantage 2.4
7, 13 -- software for Window NT
7, 13 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: km602223-at-comcast.net
Date: Wed, 14 Sep 2005 18:44:14 -0500
Subject: [Microscopy] viaWWW: Nikon Fluophot

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (km602223-at-comcast.net) from http://microscopy.com/MLFormMail.html on Wednesday, September 14, 2005 at 18:36:15
---------------------------------------------------------------------------

Email: km602223-at-comcast.net
Name: Kathleen McMillan

Title-Subject: [Filtered] fluorescence microscope

Question: If anyone has experience with the old Nikon Fluophot, is it possible to remove/replace the epi-fluorescence filters in the turret or were they glued in place?

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: kenconverse-at-qualityimages.biz
Date: Wed, 14 Sep 2005 20:27:40 -0500
Subject: [Microscopy] Gas for WD spectrometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Skage,
Yes, P-90 is 90% methane and 10% argon (and is flammable). Sounds like
you've got a thin window detector running at low absolute pressure for light
elements. That setup needs a lot of quenching gas (methane) and not much
ionizing gas (argon) to work well.

Ken Converse

Please note our new contact information.

QUALITY IMAGES
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Since 1981
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207-647-4348
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kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: SHem-at-laurentian.ca [mailto:SHem-at-laurentian.ca]
Sent: Wednesday, September 14, 2005 3:45 PM
To: kenconverse-at-qualityimages.biz

Hi there,
We're setting up a vintage instrument, a Cambridge S-250 with
a vintage microspec WD spectrometer.

This spectrometer needs P10, which is to be expected, but also P90.
Praxair don't know what this is, logic dictates that its the inverse
composition of P10 i.e.
90% methane 10% argon. Do anyone have a similar instrument and knowledge of
this gas ?
Thanks,
Skage

_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-675-1151 x4040
cell. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html



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==============================Original Headers==============================
25, 23 -- From kenconverse-at-qualityimages.biz Wed Sep 14 20:27:40 2005
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25, 23 -- Subject: RE: [Microscopy] Gas for WD spectrometer
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From: icmicroanalysis-at-cox.net
Date: Thu, 15 Sep 2005 17:22:10 -0500
Subject: [Microscopy] viaWWW: SEM Sectioning Tools

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I kind of assumed that anyone using a TEM photo or negative would scan it
into a PC at hi-res and then use digital zoom [if necessary] with a
stylus+tablet or mouse within the image analysis program to draw round the
digitised image (I've bonded with my PC and mouse). I have just had a
discussion with Tobias (Baskin) of the University of Massachusetts Biology
Department, and he mentioned how he finds it a bit quicker using a tablet
mouse with a crosshair to accurately trace round and digitise the
photomicrograph's or photocopy's features. I notice that Wacom
(www.wacom.co.uk) have a (CAD) crosshair mouse with their Intuos 2 (A4
oversize) tablets and GTCO CalComp make even flasher tablet devices, e.g.
the Drawing board III, for a comparable price (Tobias successfully uses a
CalComp tablet) - see http://www.gtcocalcomp.com/files/BROdb3small.pdf .

I liked the trace, cut out and weigh idea. It certainly works Ok (if you
don't have 500 objects to measure per image). I have also known less
numerate colleagues using string to measure perimeter (even for circles).
Conversely I have used an epidiascope to capture a graph and a £100k image
analyser to measure the area under the curve (then FigP came along in the
1980's).

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


----- Original Message -----
X-from: {yschwab-at-titus.u-strasbg.fr}
To: {keith.morris-at-ucl.ac.uk}
Sent: Wednesday, September 14, 2005 1:45 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (icmicroanalysis-at-cox.net) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, September 15, 2005 at 14:14:08
---------------------------------------------------------------------------

Email: icmicroanalysis-at-cox.net
Name: Dave

Title-Subject: [Filtered] SEM Sectioning Tools

Question: Anyone willing to provide feedback on experiences (and recommendations) using either the Allied Cross Sectioning Tool, South Bay BiPod Polisher, or Accelerated Analysis Cross Sectioning Fixture for silicon die cross sectioning?

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: jvtaylo-at-emory.edu
Date: Thu, 15 Sep 2005 17:22:36 -0500
Subject: [Microscopy] viaWWW: JEOL load box

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jvtaylo-at-emory.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, September 15, 2005 at 16:15:04
---------------------------------------------------------------------------

Email: jvtaylo-at-emory.edu
Name: Jeannette Taylor

Organization: IM&MF/ Emory University

Title-Subject: [Filtered] JEOL load box

Question: Does anyone have a used film canister (load box) for a JEOL JEM 1210 TEM which they are willing to sell or give away? Must be in good condition. Please reply off line.

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: dawn.dawson-at-case.edu
Date: Fri, 16 Sep 2005 08:46:00 -0500
Subject: [Microscopy] viaWWW: Digital camera for small animal imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dawn.dawson-at-case.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 16, 2005 at 08:22:25
---------------------------------------------------------------------------

Email: dawn.dawson-at-case.edu
Name: Dawn Dawson

Organization: Case Western Reserve University

Title-Subject: [Filtered] MListserver: Digital camera for small animal imaging

Question: I am looking for a good digital camera that can take close up gross photos of tumors in mice.... The options for stereomicroscope set-ups are a bit pricey for my pocketbook..and not so flexible...We are currently investigating a Nikon D50 with 60 mm lens and 2.8D...Any suggestions would be appreciated...

---------------------------------------------------------------------------

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From: tonygr-at-MIT.EDU
Date: Fri, 16 Sep 2005 09:43:01 -0500
Subject: [Microscopy] Position vacancy at MIT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings -

This position is not actually in microscopy, but I hope it is close enough
that it may interest some subscribers:

The Center for Materials Science and Engineering at the Massachusetts
Institute of Technology has a vacancy, with effect from November 1st. 2005,
for a research specialist in X-ray Diffraction. A description of the
vacancy, together with MIT's employment policies, benefits, and other
information, with links for on-line application is available at the
following URL:

http://sh.webhire.com/servlet/av/jd?ai=631&ji=1651388&sn=I
or applications may be forwarded by electronic or paper mail to Ms.
Jennifer Crockett, CMSE Assistant Director, Room # 13-2106, Massachusetts
Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307,
e-mail crockett-at-mit.edu.

The application review process will be open until a qualified candidate has
been identified. Questions about this position may be addressed to the
undersigned.

Tony Garratt-Reed


***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


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From: Geoffrey_Williams-at-brown.edu
Date: Fri, 16 Sep 2005 10:04:47 -0500
Subject: [Microscopy] Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The question is primarily aimed at the Biological TEM crowd.

Has anyone experimented with using a Heat block unit for curing plastic instead of an oven? 

The reasoning is that a heat block fits in the hood more easily and can be moved out when not in use.  The hood space here is extremely limited and the oven typically has been curing Spurr's and Epon type Resins in the prep room, a practice I am not comfortable with (and neither are the Safety folks here). 

Looking at the specifications for the Dry Heat Blocks some are +/- 2ºC stability wise in the $200 range with the fanciest models having +/- 0.5ºC range stability. 

Yes I am aware of the limitations, specifically sample number, no it wouldn't work for constant routine samples where there's a constant flux, but for individual processes where no more than one or two groups of samples are cured at a time it would seem reasonable.

Thoughts? Experiences? Conjecture? Opinions?

Thanks,
Geoff Williams
Facility Manager
Leduc Bioimaging Facility
Brown University
 
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



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From: phillipst-at-missouri.edu
Date: Fri, 16 Sep 2005 10:13:40 -0500
Subject: [Microscopy] Re: Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have never done it but suspect it would work. maybe even better than an
oven. Sus Ito, one of the great early TEM guys, once told me how he use to
drive from Woods Hole back to Harvard in Boston and he would tape his
tissue samples in liquid resin to his engine block so that he could section
them upon his arrival! I just wonder how you write the Materials and
Methods description of that up!


At 10:05 AM 09/16/05, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




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11, 23 -- From: Tom Phillips {phillipst-at-missouri.edu}
11, 23 -- Subject: Re: [Microscopy] Curing TEM Epoxy in Heat Blocks?
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From: gary-at-gaugler.com
Date: Fri, 16 Sep 2005 10:23:13 -0500
Subject: [Microscopy] Re: viaWWW: Digital camera for small animal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Take a look at D100. Also consider the 105/2.8 AF-D micro Nikkor
and the SB-29s macro speedlight.

gary g.


At 06:51 AM 9/16/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: jfactor-at-ns.purchase.edu
Date: Fri, 16 Sep 2005 10:52:20 -0500
Subject: [Microscopy] viaWWW: Digital camera for small animal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Geoff

I apologise for stating the obvious, but have you thought about
purchasing a more compact oven from one of the e.m. suppliers. These
would at least be capable of holding flat embedding moulds as well as
capsules at a uniform temperature.

We purchased one with external dimensions of 400mm x 330mm x 300mm
although there was a more compact version of 335mm x 305mm x 230mm in
the UK.

I certainly agree with your concerns about polymerising Spurrs/any
epoxides resins in
the lab. I stopped doing it over 20 years ago. One other possibility
would be to find a well vented outhouse/shed if your safety people
would be happier with that.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
University of Sunderland
Tyne & Wear
SR1 3SD
UK



----- Original Message -----
X-from: Geoffrey_Williams-at-brown.edu

The Nikon D70 has most (all?) of the features of the D100 (and some
additional ones?) at a less-than-$1000 price tag. I've had good
experience using it for macro photography, combined with the 60mm macro
lens (with an effective focal length of about 85mm) and a Nikon SB600
strobe. The 105mm macro lens (with an effective focal length of about
150mm) will give more working distance, but is a bit harder to
hand-hold (this may not be a problem if you put it on a stand).
--Jan Factor

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College, State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



gary-at-gaugler.com wrote:

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From: neuberger1234-at-comcast.net
Date: Fri, 16 Sep 2005 13:18:54 -0500
Subject: [Microscopy] RE: viaWWW: Digital camera for small animal imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dawn,

The D-50 is a good choice with the 60mm Micro Nikkor f2.8. I have the D2X
and same lens for close up macrophotography of flowers and other plant parts
for the Chicago Botanic Garden and it is superb but the raw files are 70MB
per image! What ever camera you buy, be sure that it can take raw images.
Also, if you decide to buy a fixed lens camera, be sure that it is the kind
that has a LCD viewfinder in addition to the LCD screen in the back of the
camera. For close up photography, you want to see what the lens sees and
the LCD on the back of the camera is often hard to see in room light (and
outdoors); you can buy hoods but that's another item.

However, there is another issue and that will be lighting. Using photo
flood lamps, halogen or other "always on" illumination produce a lot of heat
and specimens can quickly dry out and the heat can get to you in a room.
Been there done that! One choice for the Nikon is two SB800 flash units
mounted on a bracket on either side of the lens. That should give you auto
exposure. Another option (and perhaps better) is the ring flash but Nikon's
is not auto exposure (yet) but Sigma Photo makes one that is supposed to be
auto with the Nikon digital cameras but you should contact Sigma
http://www.sigmaphoto.com/flashes/flashes_flashes_details.asp?id=3258 to
discuss.

I don't know how close you will have to get to photograph those tumors but
if you will have to be inches from the specimen, the auto flash will become
a problem in that you may have to start using ND filters. Another option
might be to use the 105 mm Micro Nikkor which will give you a little more
working distance but it costs more.

Disclaimer: Nikon is only my preference but other manufacturers make equally
suitable cameras.


Damian Neuberger, Ph.D.
Consultant
Microscopy/Digital Imaging/Image Analysis
2416 Covert Rd
Glenview IL 60025
Tel: (847) 998-8574
email: neuberger1234-at-comcast.net {mailto:neuberger1234-at-comcast.net}


Email: dawn.dawson-at-case.edu
Name: Dawn Dawson

Organization: Case Western Reserve University

Title-Subject: [Filtered] MListserver: Digital camera for small animal
imaging

Question: I am looking for a good digital camera that can take close up
gross photos of tumors in mice.... The options for stereomicroscope set-ups
are a bit pricey for my pocketbook..and not so flexible...We are currently
investigating a Nikon D50 with 60 mm lens and 2.8D...Any suggestions would
be appreciated...



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From: paul_hazelton-at-umanitoba.ca
Date: Fri, 16 Sep 2005 13:50:19 -0500
Subject: [Microscopy] Re: Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Goeff

You ask a question which is actually quite interesting, and applicable
on a number of levels. First, of course, is would a block work. I will
assume you mean something like the sand blocks we use in the other (one
of my 2 non-EM homes) lab. As you note, there would be limitations, but
the temperature control on all sand blocks I’ve ever worked with is a
lot better than any oven I’ve used. You just have to take the time to
set the temperature. As Tom Phillips noted, there are plenty of
examples of alternative systems for providing the polymerization
temperatures, so to join the chorus, I see no reason why not. And as
far as using the car engine, I even remember a book about cooking while
you drive which came out in the 70's, engine block potroast and all.

The interesting part of the question as I see it is: why would you want
to put the block into a hood. Do you mean a fume hood, so you could use
the block for an intermediate step in infiltration, with low temperature
heating to assist in driving off transitional solvents? If so, it is an
interesting idea, one worth some thought. Could be quite useful.

Alternatively, do you mean a laminar flow hood for containment at a
BSL-2 level or higher. This is the most interesting potential
application. Those of us who work with emerging diseases groups, or
with infectious pathogens at the BSL-3 or BSL-4 levels are confronted
with a number of safety issues that this concept could address. Some of
my collaborators work at higher levels. They fix with a modification of
Karnovsky’s fix (we use 2% paraform/2%glut in cacodylate). But their
safety people will not let them take the material out of containment for
further processing until the samples have been in the fixative for 30
days. I feel this may lead to some deterioration of the samples, and
that there is no evidence that the pathogens are not inactivated in
hours, and so do not like this. But safety people will not let them do
otherwise.

There are too many other things to do these days for me to give up 4-6
hour blocks to go work in containment, so I’m not too keen on taking
spacesuit training - it would be fun and really interesting, but there
is just not enough time. Your question raises a lot of ideas which can
address the problems of processing, permit good technique in processing,
and meet the demands on the biosafety level.

Paul


Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926


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From: gary-at-gaugler.com
Date: Fri, 16 Sep 2005 14:43:06 -0500
Subject: [Microscopy] Re: viaWWW: Digital camera for small animal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The macro lenses work best with the macro ring flash
SB-29s. It costs about the same as two regular speed lights.
However, its big advantage is adjustable power and adjustable
shades over the flash lamps.

gary g.



At 08:54 AM 9/16/2005, you wrote:



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From: marc.pypaert-at-yale.edu
Date: Fri, 16 Sep 2005 14:44:42 -0500
Subject: [Microscopy] Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Paul,

The reason Geoff wants to put the heat block into the hood is
probably the same reason why I would never polymerize resin
outside the hood: fumes released during this process must be
extremely toxic.
And as far as finding a very small oven that would easily fit into
a hood, this is not so easy. We bought the smallest we could find,
but it is still more than 1 foot wide and deep, which takes too
much room. We in fact have been using a second, smaller oven,
that is really just a box fitted on top of a hot plate! It is very
similar
in design to the heat block Geoff wants to use. The temperature
inside is very constant, the only drawback being that you need
to calibrate the temperature control button. This only has to be
done once. I think the idea of using heat blocks is very elegant,
and I don't see why it shouldn't work with Eppendorf tubes or
even Beem capsules. For flat embedding in moulds however I
don't see how you would manage it.

Good thread, Geoff. My guess is that if nobody has tried this
before, you should give it a shot and report to the list how it went!

Marc


On Friday, September 16, 2005, at 02:51 PM, paul_hazelton-at-umanitoba.ca
wrote:

}
}
}
} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
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}
} Goeff
}
} You ask a question which is actually quite interesting, and applicable
} on a number of levels. First, of course, is would a block work. I
} will
} assume you mean something like the sand blocks we use in the other (one
} of my 2 non-EM homes) lab. As you note, there would be limitations,
} but
} the temperature control on all sand blocks I’ve ever worked with is a
} lot better than any oven I’ve used. You just have to take the time to
} set the temperature. As Tom Phillips noted, there are plenty of
} examples of alternative systems for providing the polymerization
} temperatures, so to join the chorus, I see no reason why not. And as
} far as using the car engine, I even remember a book about cooking while
} you drive which came out in the 70's, engine block potroast and all.
}
} The interesting part of the question as I see it is: why would you want
} to put the block into a hood. Do you mean a fume hood, so you could
} use
} the block for an intermediate step in infiltration, with low
} temperature
} heating to assist in driving off transitional solvents? If so, it is
} an
} interesting idea, one worth some thought. Could be quite useful.
}
} Alternatively, do you mean a laminar flow hood for containment at a
} BSL-2 level or higher. This is the most interesting potential
} application. Those of us who work with emerging diseases groups, or
} with infectious pathogens at the BSL-3 or BSL-4 levels are confronted
} with a number of safety issues that this concept could address. Some
} of
} my collaborators work at higher levels. They fix with a modification
} of
} Karnovsky’s fix (we use 2% paraform/2%glut in cacodylate). But their
} safety people will not let them take the material out of containment
} for
} further processing until the samples have been in the fixative for 30
} days. I feel this may lead to some deterioration of the samples, and
} that there is no evidence that the pathogens are not inactivated in
} hours, and so do not like this. But safety people will not let them do
} otherwise.
}
} There are too many other things to do these days for me to give up 4-6
} hour blocks to go work in containment, so I’m not too keen on taking
} spacesuit training - it would be fun and really interesting, but there
} is just not enough time. Your question raises a lot of ideas which can
} address the problems of processing, permit good technique in
} processing,
} and meet the demands on the biosafety level.
}
} Paul
}
}
} Paul R. Hazelton, PhD
} Electron Microscope Unit
} University of Manitoba
} Department of Medical Microbiology
} 531 Basic Medical Sciences Building
} 730 William Avenue
} Winnipeg, Manitoba, Canada, R3E 0W3
} e-mail: paul_hazelton-at-umanitoba.ca
} Phone:204-789-3313
} Pager:204-931-9354
} Cell:204-781-1502
} Fax:204-789-3926
}
}
} ==============================Original
} Headers==============================
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}

--
Marc Pypaert
Department of Cell Biology
Center for Cell and Molecular Imaging
Ludwig Institute for Cancer Research
Yale University School of Medicine
333 Cedar Street, PO Box 208002
New Haven, CT 06520-8002
TEL 203-785 3681
FAX 203-785 7446



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From: glenmac-at-u.washington.edu
Date: Fri, 16 Sep 2005 15:09:53 -0500
Subject: [Microscopy] Re: Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When our current was being designed, I specified one bench with an
awning hood to vent fumes from solvent dishes, paraffin baths and
embedding ovens. The 30" deep bench has a 3" gap between the
backsplash and wall. The ovens are beneath the bench and their fumes
are pulled up behind the backsplash. The stainless steel awning is
28" above the bench with a plexiglas skirt extending 4" below the
awning to increase face flow.

A heat block is likely much cheaper than remodeling one's lab.

Glen
On Sep 16, 2005, at 12:46 PM, marc.pypaert-at-yale.edu wrote:

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} Hi Paul,
}
} The reason Geoff wants to put the heat block into the hood is
} probably the same reason why I would never polymerize resin
} outside the hood: fumes released during this process must be
} extremely toxic.
} And as far as finding a very small oven that would easily fit into
} a hood, this is not so easy. We bought the smallest we could find,
} but it is still more than 1 foot wide and deep, which takes too
} much room. We in fact have been using a second, smaller oven,
} that is really just a box fitted on top of a hot plate! It is very
} similar
} in design to the heat block Geoff wants to use. The temperature
} inside is very constant, the only drawback being that you need
} to calibrate the temperature control button. This only has to be
} done once. I think the idea of using heat blocks is very elegant,
} and I don't see why it shouldn't work with Eppendorf tubes or
} even Beem capsules. For flat embedding in moulds however I
} don't see how you would manage it.
}
} Good thread, Geoff. My guess is that if nobody has tried this
} before, you should give it a shot and report to the list how it went!
}
} Marc
}
}
} On Friday, September 16, 2005, at 02:51 PM, paul_hazelton-at-umanitoba.ca
} wrote:
}
}
} }
} }
} }
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} }
} } Goeff
} }
} } You ask a question which is actually quite interesting, and
} } applicable
} } on a number of levels. First, of course, is would a block work. I
} } will
} } assume you mean something like the sand blocks we use in the other
} } (one
} } of my 2 non-EM homes) lab. As you note, there would be limitations,
} } but
} } the temperature control on all sand blocks I’ve ever worked with is a
} } lot better than any oven I’ve used. You just have to take the
} } time to
} } set the temperature. As Tom Phillips noted, there are plenty of
} } examples of alternative systems for providing the polymerization
} } temperatures, so to join the chorus, I see no reason why not. And as
} } far as using the car engine, I even remember a book about cooking
} } while
} } you drive which came out in the 70's, engine block potroast and all.
} }
} } The interesting part of the question as I see it is: why would you
} } want
} } to put the block into a hood. Do you mean a fume hood, so you could
} } use
} } the block for an intermediate step in infiltration, with low
} } temperature
} } heating to assist in driving off transitional solvents? If so, it is
} } an
} } interesting idea, one worth some thought. Could be quite useful.
} }
} } Alternatively, do you mean a laminar flow hood for containment at a
} } BSL-2 level or higher. This is the most interesting potential
} } application. Those of us who work with emerging diseases groups, or
} } with infectious pathogens at the BSL-3 or BSL-4 levels are confronted
} } with a number of safety issues that this concept could address. Some
} } of
} } my collaborators work at higher levels. They fix with a modification
} } of
} } Karnovsky’s fix (we use 2% paraform/2%glut in cacodylate). But their
} } safety people will not let them take the material out of containment
} } for
} } further processing until the samples have been in the fixative for 30
} } days. I feel this may lead to some deterioration of the samples, and
} } that there is no evidence that the pathogens are not inactivated in
} } hours, and so do not like this. But safety people will not let
} } them do
} } otherwise.
} }
} } There are too many other things to do these days for me to give up
} } 4-6
} } hour blocks to go work in containment, so I’m not too keen on taking
} } spacesuit training - it would be fun and really interesting, but
} } there
} } is just not enough time. Your question raises a lot of ideas
} } which can
} } address the problems of processing, permit good technique in
} } processing,
} } and meet the demands on the biosafety level.
} }
} } Paul
} }
} }
} } Paul R. Hazelton, PhD
} } Electron Microscope Unit
} } University of Manitoba
} } Department of Medical Microbiology
} } 531 Basic Medical Sciences Building
} } 730 William Avenue
} } Winnipeg, Manitoba, Canada, R3E 0W3
} } e-mail: paul_hazelton-at-umanitoba.ca
} } Phone:204-789-3313
} } Pager:204-931-9354
} } Cell:204-781-1502
} } Fax:204-789-3926
} }
} }
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} } 9, 19 -- Date: Fri, 16 Sep 2005 13:50:08 -0500
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} }
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} }
}
} --
} Marc Pypaert
} Department of Cell Biology
} Center for Cell and Molecular Imaging
} Ludwig Institute for Cancer Research
} Yale University School of Medicine
} 333 Cedar Street, PO Box 208002
} New Haven, CT 06520-8002
} TEL 203-785 3681
} FAX 203-785 7446
}
}
}
} ==============================Original
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} 10, 19 -- From marc.pypaert-at-yale.edu Fri Sep 16 14:44:41 2005
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} 10, 19 -- Date: Fri, 16 Sep 2005 15:44:39 -0400
} 10, 19 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
} 10, 19 -- Subject: Re: [Microscopy] Re: Curing TEM Epoxy in Heat
} Blocks?
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From: macaluso-at-aecom.yu.edu
Date: Fri, 16 Sep 2005 16:03:40 -0500
Subject: [Microscopy] Re: Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Geoff,
Ladd Research makes a Dri Block Oven for curing
Beem caps and flat molds. We used one regularly
some time ago. It is compact and the temperature was stable.
Frank

At 11:06 AM 9/16/2005, you wrote:



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From: Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Fri, 16 Sep 2005 17:49:27 -0500
Subject: [Microscopy] SEM roughness measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

Does anyone have experience with deriving roughness information from SEM
images? I presume you need to use stereo pairs to get 3D information
from a rough substrate.

I would be especially interested if anyone had a free/cheap software
package that would handle this task.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



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From: PWebster-at-hei.org
Date: Fri, 16 Sep 2005 18:01:02 -0500
Subject: [Microscopy] Re: Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

It appears to me that the problem to be solved is to find a way to
polymerize resin but protect staff personnel from the fumes that are given
off during heating yet find a space-saving solution.

A simple approach would be to only use sealed molds such as Eppendorf tubes
and BEEM capsules. The oven can thus be placed anywhere in the lab.
However, I do know that many people prefer the ease and lower cost of
re-useable flat molds.

I have been experimenting with polymerizing resins using a microwave oven.
With variable results. However, with some formulations of resin it is
possible to polymerize in a flat mold in less than 2hr.

I have used a laboratory-grade microwave connected to an exhaust duct (which
is very convenient) and also with a regular kitchen microwave. The end
result is, if the resin is going to polymerize, the process will work in
either machine.

Not all resin recipes work and even fewer of them can be polymerized in a
flat mold. It may be worth giving this approach a try.

The best part of sing the microwave is that the exhaust duct allows us to
place the machine far from the chemical extractor hoods. Connecting a
regular oven to an exhaust duct may also be a reasonably effective solution.
Our convection oven has a wide duct in the top to which metal ducting,
similar to that connected to household clothes dryers, can be connected.

Best regards,

Paul Webster



Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org


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From: redhair-at-stanford.edu
Date: Fri, 16 Sep 2005 18:30:15 -0500
Subject: [Microscopy] Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all
I am wondering reading these e-mails, does anyone really know how
noxious the fumes are that are released from an embedding oven? We
too are pressed for space in the hood so I've moved the embedding
oven into a not heavily populated corner in a large
lab. We polymerize LR White and epoxy resin blocks-5-10 blocks
worth maybe once a week at most. I can't smell any fumes in there (
unlike the mercaptoethanol or ETT the molecular biologists regularly
use). Am I exposing a room full of people to something bad? Is it the
quantity of blocks that one needs to worry about? The microwave is
great for processing (hooked up to the fume hood via the duct) but I
don't ant to baby sit it for 2 hours to get a perfectly hardened
epoxy flat block.
Safety conscious in CA
JoAnn Buchanan



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From: Mike.Bode-at-soft-imaging.net
Date: Fri, 16 Sep 2005 18:31:21 -0500
Subject: [Microscopy] SEM roughness measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Daniel,

Yes, you can use stereo pairs to calculate a surface profile and from there calculate roughness parameters. We have a module for our analySIS software that accomplishes this. If you want more information, please contact me by email.

There are certain limitations to what you can do with this technique. The z-resolution depends on a number of factors, the most important of which is the tilt angle. For typical tilt angles of 6 - 10 degrees, the resolution is roughly 1/10th of the lateral resolution (you can calculate that by multiplying the lateral resolution with the tan of the stereo angle). For example: If your stereo images have a resolution of 1 micron (1mm x 1mm field of view and an image resolution of 1000 x 1000 pixels), your z-resolution will be on the order of 10 microns. In order to evaluate if the technique will do what you want, you need the following information: required field of view, image resolution, stereo angle.

Let me know if you need further info.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca]
Sent: Friday, September 16, 2005 4:53 PM
To: Mike Bode

Hi everyone,

Does anyone have experience with deriving roughness information from SEM images? I presume you need to use stereo pairs to get 3D information from a rough substrate.

I would be especially interested if anyone had a free/cheap software package that would handle this task.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



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From: DrJohnRuss-at-aol.com
Date: Fri, 16 Sep 2005 18:41:28 -0500
Subject: [Microscopy] Re: SEM roughness measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 9/16/05 6:50:20 PM, Daniel.Salamon-at-nrc-cnrc.gc.ca writes:

} Does anyone have experience with deriving roughness information from SEM
} images? I presume you need to use stereo pairs to get 3D information
} from a rough substrate.
}
} I would be especially interested if anyone had a free/cheap software
} package that would handle this task.

I don't know of any free stuff. The least expensive is probably the Fovea Pro
software from Reindeer (www.ReindeerGraphics.com), which has a demo version
you can download that both fuses stereo pairs and performs a variety of surface
roughness measurements. Several other companies offer routines that derive
elevation from stereo pairs, but few also perform roughness measurements. The
most versatile, complete and probably accurate program I've seen is the Alicona
package (www.alicona.com), which has a demo version you can download. But it's
a long way from free.


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From: Jerzy.Gazda-at-ceriumlabs.com
Date: Sat, 17 Sep 2005 09:59:41 -0500
Subject: [Microscopy] viaWWW:SEM/FIB/TEMprep technologist position

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CeriumLaboratories LLC, a subsidiary of AMD Inc., is currently seeking a
technologist to support analytical electron microscopy section.
Successful candidate will be supporting materials analysis activates
using optical, SEM, FIB, and TEM microscopes. Work will involve
interaction with engineers and/or customers to formulate analytical
approaches, sample preparation, and SEM/FIB imaging.

Preferred education includes Associate Degree in Engineering with
emphasis on math, chemistry, physics, or materials science. A minimum
of three years work experience in the field of sample preparation for
microscopy and microanalysis of materials. The position requires strong
interpersonal skills.

The job is located in Austin, TX. For more information on CeriumLabs
please visit our website at www.ceriumlabs.com .


The full description of the position can be found at:
http://www2.amd.com/us-en/Job_Opps/Display/1,,330,00.html

Please e-mail your resume to Hafeez.Khan-at-amd.com and CC jobs-at-amd.com.

Please be sure to include the req# T54856 in the subject line.


******************************************************
Jerzy Gazda, Ph.D. CeriumLaboratories L.L.C.

Supervising Engineer 5204 E. Ben White
Blvd. - MS 512
Austin, TX
78741=20
TEL: 1-800-538-8450, Ext. 51453 =20
jerzy.gazda-at-ceriumlabs.com
******************************************************

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From: peter.gothro-at-fda.gov
Date: Sat, 17 Sep 2005 13:20:12 -0500
Subject: [Microscopy] viaWWW:Digital camera for small animal imaging

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (peter.gothro-at-fda.gov) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 16, 2005 at 13:43:28
---------------------------------------------------------------------------

Email: peter.gothro-at-fda.gov
Name: Peter Gothro

Organization: US Food and Drug Administration

Title-Subject: [Filtered] Digital camera for small animal imaging

Question: I don't know how far you're into building stuff for macrophotography, but this is a WAY cool site. Much of it is in german. There are also areas dealing with equipment.

http://users.skynet.be/fotoopa/macro_set.htm

Cheers,
Pete

Peter W. Gothro, Entomologist
FDA PRL-NW
22201 23rd Dr SE
Bothell, WA 98021-4421
425/402-3176 - Voice
425/483-4996 - Fax
Peter.Gothro-at-fda.gov

This e-mail message is intended for the exclusive use of the recipient(s) named above. It may contain information that is protected, privileged, or confidential. If you are not the intended and authorized recipient, any dissemination, distribution, or copying is strictly prohibited. If you received this e-mail in error, please e-mail the sender immediately at peter.gothro-at-fda.gov.



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From: donc-at-asmicro.com
Date: Sun, 18 Sep 2005 11:35:58 -0500
Subject: [Microscopy] RE: SEM roughness measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike Bode described a way of estimating the vertical resolution that can be
achieved by using stereo pairs. His trigonometric calculation predicts that
the practical vertical resolution is 10x worse than the lateral resolution,
for tilt angles of 6-10 degrees. I wonder whether users of various SEM
measurement tools have the same experience in actual practice. And I wonder
what the observed limits of vertical resolution are for the highest
resolution FE-SEMs.
regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]

----- Original Message -----
From: Mike.Bode-at-soft-imaging.net
To: donc-at-asmicro.com
Sent: Friday, September 16, 2005 6:34 PM
Subject: [a] [Microscopy] RE: SEM roughness measurement





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Hello Daniel,

Yes, you can use stereo pairs to calculate a surface profile and from
there calculate roughness parameters. We have a module for our analySIS
software that accomplishes this. If you want more information, please
contact me by email.

There are certain limitations to what you can do with this technique. The
z-resolution depends on a number of factors, the most important of which is
the tilt angle. For typical tilt angles of 6 - 10 degrees, the resolution is
roughly 1/10th of the lateral resolution (you can calculate that by
multiplying the lateral resolution with the tan of the stereo angle). For
example: If your stereo images have a resolution of 1 micron (1mm x 1mm
field of view and an image resolution of 1000 x 1000 pixels), your
z-resolution will be on the order of 10 microns. In order to evaluate if the
technique will do what you want, you need the following information:
required field of view, image resolution, stereo angle.

Let me know if you need further info.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca
[mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca]
Sent: Friday, September 16, 2005 4:53 PM
To: Mike Bode
Subject: [Microscopy] SEM roughness measurement




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Hi everyone,

Does anyone have experience with deriving roughness information from SEM
images? I presume you need to use stereo pairs to get 3D information from a
rough substrate.

I would be especially interested if anyone had a free/cheap software
package that would handle this task.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116
Street Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



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From: grant-at-cmosxray.com
Date: Sun, 18 Sep 2005 16:35:21 -0500
Subject: [Microscopy] LM - Microscope for silicon wafer inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Could anyone recomend a microscope for silicon inspection? I don't have more than about $900 for this project. I found this forum by searching in google for IC decapping information.

I'd like the microscope provide better images than these:
http://media.diywelder.com/images3/091205-MMILogo_IMGP2051.jpg
http://media.diywelder.com/images3/091205-RightOfMap_IMGP2068.jpg
http://media.diywelder.com/images3/091205-wholechip_IMGP2074.jpg

Those were taken with an old zeiss microscope at UAA (university of alaska anchorage). Its a triocular microscope with CCD camera, but I captured those images looking through the eyepiece with an economy pentax digital camera.

I've found two interesting models:

http://cgi.ebay.com/METALLURGICAL-METALLOGRAPHIC-MICROSCOPE-USB-PC-CCD_W0QQitemZ7546614326QQcategoryZ26411QQcmdZViewItem

There are a few here that look good:

http://www.bargainmicroscopes.com/scopesmenu/product.php?id=81&pcat=&pid=

Is there anything I should be aware of?

I want to get crystal clear images of the silicon. Here is a 20 mega
pixel example of what I can get by holding my digital camera to the
eyepiece. Its fuzzy on the right because I had to "aim" at an angle
into the eyepiece to avoid strange hazing. Warning...2mb JPEG

http://media.diywelder.com/images3/091705-ASG-fuseblock-pano-fullres-cropped.jpg

Thanks,
Grant


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From: gary.nichols-at-pfizer.com
Date: Mon, 19 Sep 2005 02:58:46 -0500
Subject: [Microscopy] SEM roughness measurement

Contents Retrieved from Microscopy Listserver Archives
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Dear Daniel

Although not free (costs about £5000), the MeX software developed by Alicona
(see www.alicona.com ) is an excellent way to derive roughness measurements
(and lots more) using SEM. You can use stereo pairs, but the new Tricreator
uses three images tilted relative to each for greater accuracy (which can't
be achieved by reading the tilt angle from a specimen stage).
---------------------------------------------------
Gary Nichols,
Material Sciences,
Pharmaceutical R&D (D435),
Pfizer Global R&D,
Ramsgate Road,
Sandwich,
Kent
CT13 9NJ,
UK
e-mail: gary.nichols-at-pfizer.com
tel: +44 (0)1304 643925
fax: +44 (0)1304 656726

---------------------------------------------------
Gary Nichols,
Material Sciences,
Pharmaceutical R&D (D435),
Pfizer Global R&D,
Ramsgate Road,
Sandwich,
Kent
CT13 9NJ,
UK
e-mail: gary.nichols-at-pfizer.com
tel: +44 (0)1304 643925
fax: +44 (0)1304 656726
----------------------------------------------------
LEGAL NOTICE

Unless expressly stated otherwise, this message is confidential and
may be privileged. It is intended for the addressee(s) only. Access to
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-----Original Message-----
X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca
[mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca]
Sent: 16 September 2005 23:54
To: Nichols, Gary

Hi everyone,

Does anyone have experience with deriving roughness information from SEM
images? I presume you need to use stereo pairs to get 3D information
from a rough substrate.

I would be especially interested if anyone had a free/cheap software
package that would handle this task.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



==============================Original Headers==============================
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From: K.venner-at-ion.ucl.ac.uk
Date: Mon, 19 Sep 2005 07:54:22 -0500
Subject: [Microscopy] viaWWW: epoxy resin fume toxicity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (K.venner-at-ion.ucl.ac.uk) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 19, 2005 at 05:49:23
---------------------------------------------------------------------------

Email: K.venner-at-ion.ucl.ac.uk
Name: k.venner

Organization: Institute of Neurology, UCL, London UK

Title-Subject: [Filtered] epoxy resin fume toxicity:

Question: Please be very wary of venting the oven curing the resin blocks anywhere other than in the fume hood. We have one lab Tech who is unable to work with em resins at all due to extreme sensitization several years ago. Recently, one worker decided to cure some resin blocks in another lab in an oven outside of the fume hood; within hours her eyes had swelled so badly that she could not see. She was only working in the vicinity, but in a big, well ventilated open plan lab area. Once senstization has occurred, it is always potentially very serious, for anyone exposed to the fumes and not necessarily technical staff working in EM. Incidentally, resin dust makes my fingers swell within minutes of handling/sawing small blocks, even wearing gloves,so I avoid this practice now. Hope my comments help.

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: SHem-at-laurentian.ca
Date: Mon, 19 Sep 2005 10:32:48 -0500
Subject: [Microscopy] Jeol JSM-6400

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everyone,
As the list members have been so helpful in past, I try my luck again.

I have an old Jeol JSM-6400, which were accidentally vented close to one
of the pirani gages. I suspect this action damaged the pirani gage as the relevant valve
wont open even if the attached penning gage records a good vacuum in the chamber in question.
Anyone had similar expiriences ? and most importantly do anyone have electrical schematics for a Jeol JSM-6400 ?

Thanks,
Skage



_______________________________________________________________

Skage Hem
Research Scientist, Ph.D.
CAF, Department of Earth Sciences
Laurentian University
Ramsey Lake Road
Sudbury, ON
Canada
P3E 2C6

ph. 705-675-1151 x4040
cell. 705-562-7321
fax 705-675-4898

http://laurentian.ca/geology/Research/hem.html



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From: rjharris-at-uwo.ca
Date: Mon, 19 Sep 2005 12:17:59 -0500
Subject: [Microscopy] Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Geoff
We were faced with the same type of space limitation in our hood. What
we've done is vented our oven to the fumehood's duct via the thermometer
hole (we removed the thermometer adaptor which left a 3" hole) on the top
using 4" diameter metal ductwork. There is a damper control in the line
allowing us to adjust the rate of exhaust.
Voila! No new hood needed, more room in the hood, no potentially noxious
fumes in the lab. FYI the vapours from curing epoxies are noxious, and can
cause susceptible people to develop mucous membrane irritations and
swelling.

Cheers!
Richard Harris
Laboratory Supervisor,
Imaging and Analysis
Department of Biology,
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph. 519-661-2111 ext. 86780
Fax 519-661-3935


-----Original Message-----
X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu]
Sent: Friday, September 16, 2005 11:09 AM
To: rjharris-at-uwo.ca

The question is primarily aimed at the Biological TEM crowd.

Has anyone experimented with using a Heat block unit for curing plastic
instead of an oven? 

The reasoning is that a heat block fits in the hood more easily and can be
moved out when not in use.  The hood space here is extremely limited and the
oven typically has been curing Spurr's and Epon type Resins in the prep
room, a practice I am not comfortable with (and neither are the Safety folks
here). 

Looking at the specifications for the Dry Heat Blocks some are +/- 2ºC
stability wise in the $200 range with the fanciest models having +/- 0.5ºC
range stability. 

Yes I am aware of the limitations, specifically sample number, no it
wouldn't work for constant routine samples where there's a constant flux,
but for individual processes where no more than one or two groups of samples
are cured at a time it would seem reasonable.

Thoughts? Experiences? Conjecture? Opinions?

Thanks,
Geoff Williams
Facility Manager
Leduc Bioimaging Facility
Brown University
 
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



==============================Original Headers==============================
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From: Geoffrey_Williams-at-brown.edu
Date: Mon, 19 Sep 2005 16:21:29 -0500
Subject: [Microscopy] Curing TEM Epoxy in Heat Blocks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Richard,

Venting the oven to the current hood was the first consideration. We do have a very nice oven, it works well. But there is little to no counter space near the hood and to tie the oven exhaust into the existing hood in the lab would first require a Facilities Management Feasibility study, and more than likely a few thousand dollars in modification, mostly because it has to be certified to draw a specific amount of air and also to not affect the functioning of the rest of the hood.

I didn't realize that Ladd sold a dry heat curing device when I wrote the email. However, not to put a price tag on anything, I want to try either one of the economical units on the market or ideally borrow one from a lab for the trial run.

And sealed capsules are not vapor free. For a brief idea on the toxicity of the chemicals in any of the most common epoxy/resins read the warnings on the bottles. It is how I always started the lab portion in TEM when we got to mixing the Spurr's. Nothing like getting the attention of students by talking about central nervous system toxins.

Thanks for the feedback everyone,

Geoff

-----Original Message-----
X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca]
Sent: Monday, September 19, 2005 1:26 PM
To: Williams, Geoffrey

Hello Geoff
We were faced with the same type of space limitation in our hood. What
we've done is vented our oven to the fumehood's duct via the thermometer
hole (we removed the thermometer adaptor which left a 3" hole) on the top
using 4" diameter metal ductwork. There is a damper control in the line
allowing us to adjust the rate of exhaust.
Voila! No new hood needed, more room in the hood, no potentially noxious
fumes in the lab. FYI the vapours from curing epoxies are noxious, and can
cause susceptible people to develop mucous membrane irritations and
swelling.

Cheers!
Richard Harris
Laboratory Supervisor,
Imaging and Analysis
Department of Biology,
University of Western Ontario,
London Ontario, CANADA.
N6A 5B7
Ph. 519-661-2111 ext. 86780
Fax 519-661-3935


-----Original Message-----
X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu]
Sent: Friday, September 16, 2005 11:09 AM
To: rjharris-at-uwo.ca

The question is primarily aimed at the Biological TEM crowd.

Has anyone experimented with using a Heat block unit for curing plastic
instead of an oven? 

The reasoning is that a heat block fits in the hood more easily and can be
moved out when not in use.  The hood space here is extremely limited and the
oven typically has been curing Spurr's and Epon type Resins in the prep
room, a practice I am not comfortable with (and neither are the Safety folks
here). 

Looking at the specifications for the Dry Heat Blocks some are +/- 2ºC
stability wise in the $200 range with the fanciest models having +/- 0.5ºC
range stability. 

Yes I am aware of the limitations, specifically sample number, no it
wouldn't work for constant routine samples where there's a constant flux,
but for individual processes where no more than one or two groups of samples
are cured at a time it would seem reasonable.

Thoughts? Experiences? Conjecture? Opinions?

Thanks,
Geoff Williams
Facility Manager
Leduc Bioimaging Facility
Brown University
 
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



==============================Original Headers==============================
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20, 28 -- From: Richard Harris {rjharris-at-uwo.ca}
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From: Judith_A_Ruiz-at-whirlpool.com
Date: Mon, 19 Sep 2005 17:22:47 -0500
Subject: [Microscopy] viaWWW:SEM vs Confocal?

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Email: Judith_A_Ruiz-at-whirlpool.com
Name: Judith Ruiz

Title-Subject: [Filtered] MListserver:

Question: My boss is asking me to investigate the pros and cons of a confocal microscope. Her thought is to not get the new SEM but get a Confocal. Our SEM is 20 years old and I'm really hoping to get a variable pressure unit soon. Anyone have any thoughts on this. What are the restrictions of the confocal. We are a materials/industrial forensics lab and the confocal is to be used to surface evaluations of coatings, etc.

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==============================Original Headers==============================
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From: dawn.dawson-at-case.edu
Date: Mon, 19 Sep 2005 17:23:14 -0500
Subject: [Microscopy] viaWWW: Thanks Responses to inquiry re digital imaging

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Email: dawn.dawson-at-case.edu
Name: Dawn Dawson

Organization: Case Western Reserve University

Title-Subject: [Filtered] MListserver: Responses to inquiry re digital imaging

Question: Thanks to all who responded with advice on small animal digital imaging...your comments were most appreciated and helpful.

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From: mrsquinlin-at-gmail.com
Date: Mon, 19 Sep 2005 17:38:56 -0500
Subject: [Microscopy] AskAMicroscopist: confocal and fluorescence microscopy career

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Email: mrsquinlin-at-gmail.com
Name: Brandi Quinlin

Organization: SOSU

Education: Graduate College

Location: Durant OK

Question: I have recently been involved in some research for school at the Noble Foundation using their confocal microscope. It has made me aware that I want to be able to do work with microscopes like the confocal and fluorescence. How do I get started on that career path?

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==============================Original Headers==============================
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From: waythepainross38-at-msn.com
Date: Mon, 19 Sep 2005 17:39:25 -0500
Subject: [Microscopy] AskAMicroscopist: difference in Brightfield, Darkfield

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Email: waythepainross38-at-msn.com
Name: Valerie

Organization: Rio Salado

Education: Undergraduate College

Location: Phoenix AZ USA

Question: What are the difference in Brightfield, Darkfield and phase contrast microscopes? and how do organisms appear in each of these?

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: dcrippen-at-buckinstitute.org
Date: Mon, 19 Sep 2005 21:09:29 -0500
Subject: [Microscopy] viaWWW: Constant temp enclosure

Contents Retrieved from Microscopy Listserver Archives
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All,

Discussing the importance of safe handling of toxic vapors, liquids, solids
(powders and dust), is not worth anything unless it is followed by actual
safe laboratory practices and equipment; this is critical to the longevity
of students and faculty. Sorry to sound like a stuck CD but toxic vapors
may do more that cause rapid sensitization or neurological damage. Think
long term and that what we handle as graduate students may hit us with
devastating diseases 30 or 40 years later. I know from experience that
vapors can do more that cause neurological damage (been there, have that)
but also damage the lungs necessitating a lung transplant, IF you are
incredibly lucky as I have been. A fellow grad student was not so lucky
with another disease. Were these caused by EM grad work and careers? No
one knows (but it is suspected by medical experts) but do you want to take
the chance?

Damian



Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dcrippen-at-buckinstitute.org) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Monday, September 19, 2005 at 19:31:32
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Email: dcrippen-at-buckinstitute.org
Name: Danielle Crippen

Organization: Buck Instititute for Age Research

Title-Subject: [Filtered] MListserver: Constant temp enclosure

Question: Hi there,

What have people done about setting up constant temperature enclosures for their Zeiss LSM 510 NLO on Axiovert 200 scopes?? We know we can purchase one from Zeiss for something like $18000.00. But we also know we can design our own, though this option is a bit difficult with the scan head box on the side, rather than the back, of the scope. We prefer to heat the whole scope, not just the stage and objectives.

Has anyone designed their own enclosure for this type of microscope?? If not, has anyone found a less expensive option than purchasing the one from Zeiss??

Any and all advice is welcome!!

Cheers,

Danielle

---------------------------------------------------------------------------

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From: klk-at-biotech.ufl.edu
Date: Tue, 20 Sep 2005 14:23:59 -0500
Subject: [Microscopy] LKB Ultrotome III 8800 manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dawn,

A colleague of mine is a keen photographer and owns the Nikon D70 -
basically the same as the D50 (which is reviewed at
http://www.pcpro.co.uk/reviews/75059/nikon-d50.html?searchString=olypus+d50+d50 )
. . Although its a great SLR camera it has no macro function at all (unlike
all the cheaper compact pro-sumer digital camera's). Thus you will probably
need the optional AF 60mm f /2.6D macro lens you mention to get closer than
2 feet or so (although even that is quoted as only going to 22cm). The AF60
f isn't a proper DX lens specifically designed for the small D50 sensor, but
I'm sure it works fine. I don't believe there is a DX 'macro' lens on the
cards. The macro lens can be used for standard photos as well but its not
quite as good as the standard lens for this. There aren't any cheap
extension tube rings available either (probably as the CCD detector is a lot
smaller that 35mm). I'd try the macro lens out at the local camera store (or
buy on approval). I have seen the macro telephoto AF 105 f/2.8 recommended
as well for this body, for 'portrait and detailed work' i.e. things a bit
further away. Also consider the highly rated Canon 350D SLR with new EF-S
60mm f/2.8 Macro lens ('built for digital').

Although I have four SLR film cameras in the cupboard I have to say I now
prefer top of the range compact cameras with active LCD viewfinders (granted
its also to do with the price and where I take pictures). Over in the UK
there is the far cheaper £340 Canonpowershot S2 IS 'compact' 5MP 12x zoom
camera (review at
http://www.pcpro.co.uk/shopper-reviews/75287/canon-powershot-s2-is.html?searchString=powershot+s2+is+powershot+s2+is
which has a 'stunning macro mode that lets you focus on objects right up to
lens face...'. If money is a tight this far cheaper option will no doubt
still produce excellent images. It is a bit like an SLR in that it has an
active LCD viewfinder showing what the CCD sees. Personally I often prefer
an active LCD viewfinder (Canon S2 IS type) to the SLR (D50) purely optical
one - you actually see the same thing as the CCD detector (clouds, glare
etc...) and you can quickly move the camera about to change the
auto-exposure lock settings - granted its viewed at relatively low
resolution although with digital gain you can check focus after the pictures
taken - and usefully you can easily view the camera setup menu within the
viewfinder. I just can't read the standard backpanel LCD display text
(without magnifying glasses) and even for images its often useless in bright
light. Nip down the camera shop and try them out ? Just add £15 for the
mini-tripod to use with the remote or self- timer in case the flash causes
wet tissue glare. However these LCD active viewfinders can lead to
occasional soft or out of focus images, which you may miss due to the lower
resolution viewfinder. So you may have to download to PC and check (not
normally awkward in a lab). This should be less of a problem with an optical
SLR camera, plus the compacts have fewer, if any, filter or lens options.

So that sounds another option to the Nikon D50 (I don't suppose you will be
disappointed with the far more expensive D50 + macro though). For our lab
work we make do with a heavily used sub £200 4MP Canon A80 compact.

Keith

Dr Keith J Morris
Image Facilities Manager
Cell Biology Division
The Institute of Opthalmology
UCL, 11-43 Bath Street
London EC1V 9EL.

Tel: 020 7608 4050



----- Original Message -----
X-from: {dawn.dawson-at-case.edu}
To: {keith.morris-at-ucl.ac.uk}
Sent: Friday, September 16, 2005 2:56 PM

Dear Valerie,

Standard brightfield or Kohler illumination is fine for resolution and even
sample illumination, but on many unstained and live samples it lacks the
contrast to pick out any detail. Hence it is ideal for stained sections or
specimens that have high contrast (e.g. soot particles). Both phase contrast
and dark ground illumination are optical 'contrast enhancer's and require
special optics in the objective barrel and./or condenser (although
darkground at low magnification only requires a central opaque 'patch
stop').

In phase contrast, the contrast within specimens that absorb little of the
transmitted light (e.g. live or fixed unstained cells) is significantly
increased. It works as different cell regions have different refractive
indices. Phase contrast can also be used to further enhance stained
specimens, although it is widely used for investigating live cell motility
or as a secondary source of information with fluorescence microscopy. There
is often a bright halo around the object.

In dark ground, the specimen is brightly illuminated against a black
background, and no direct light enters the objective only that scattered or
reflected by the specimen. Scattering features are bright with contrast
being higher and reversed. Although it offers no higher resolution that a
standard microscope, submicron particles such as the larger viruses can be
seen as dots of light.

There's also differential interference contrast (DIC) that gives a relief
effect and less of halo around the specimen than phase contrast optics. With
all with image contrast enhancement, the specimen and coverslips must be
clean with no air bubbles. Coloured in-line filters can also increase
contrast in stained samples, particularly with B&W hi-resolution CCD
cameras.

Contrast in a standard brightfield microscope can be increased by simply by
closing the aperture diaphragm in the condenser or moving it off-axis
(oblique illumination) giving a shadowed relief effect (which is also seen
if a desk lamp is left on next to the microscope).

See 'Introduction to light microscopy' by S Bradbury & B. Bracegirdle (Royal
Microscopy Society microscope handbook 42) or a similar text. There's also a
really excellent on-line tutorial at http://www.microscopyu.com/ that has
all the pictures and info (you need Java installed on your browser for the
tutorials).

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk
----- Original Message -----
X-from: {waythepainross38-at-msn.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Monday, September 19, 2005 11:43 PM

Hello All,

I have a manual for a LKB Ultrotome III 8800 ultramicrotome. If anyone
has a need for this manual I'll be happy to send it. I have three
manuals up for grab.

--
Karen L. Kelley
ICBR Electron Microscopy Manager
University of Florida
ICBR Electron Microscopy Core Lab
Bartram Hall Room 214
Box 118525 Gainesville Florida
Lab: 352-392-1184 fax: 352-846-0251
Email: klk-at-biotech.ufl.edu
Southeastern Microscopy Society Treasurer
http://www.biotech.ufl.edu/EM/



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From: aeckerson-at-deltacollegeprep.org
Date: Tue, 20 Sep 2005 18:47:55 -0500
Subject: [Microscopy] AskAMicroscopist: microscopes for 7th graders

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---------------------------------------------------------------------------

Email: aeckerson-at-deltacollegeprep.org
Name: Amoz Eckerson

Organization: KIPP: Delta College Preparatory School

Education: 6-8th Grade Middle School

Location: Helena, Arkansas, USA

Question: I'm looking to obtain microscopes for 7th graders in Life Science so that we can look at cells and other cool stuff. I wanted to ask:
What is the best microscope for the price?
What type of magnification will I need to see cells?
Will we be able to see individual cell parts with an optical microscope?
What companies donate microscopes to schools?

Thanks.

---------------------------------------------------------------------------

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From: icmicroanalysis-at-cox.net
Date: Wed, 21 Sep 2005 08:18:15 -0500
Subject: [Microscopy] viaWWW: Leveling platform for microscope

Contents Retrieved from Microscopy Listserver Archives
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Dear Judith,

I was under the impression that all that an SEM was really good for was for
the evaluation of the surface of non-living materials (unless you are into
vaporising bunnies). Confocal might be OK for polymer coatings, but SEM
images are rather in a different league to confocal in terms of resolution
and
clarity. There is an article on polymers at
http://fire.nist.gov/bfrlpubs/build04/art034.html .

I have occasionally viewed things under confocal that I am used to seeing
under
SEM or even under LM phase contrast with a CCD camera, like recovered
inhaled mineral fibres, pollen grains, or Nuclepore filter surfaces, and
thought wow they look really bad. We only use laser confocals here because
our delicate water based life forms aren't very happy in a vacuum (and to be
honest the confocal does come alive with intra-cellular fluorescent
markers).
In my last days at Harwell 10 years ago a new Camscan SEM was a revelation
with digital image VDU, PC control and Quantimet style image analysis,
compared to our older SEM machines (picture quality was pretty similar
though).

I'm sure the manufacturers would be very keen to demo a confocal at their
head
office (our confocals are designed for living cells). I would have thought
you
could rent time on a local confocal if it is needed occasionally (we charge
£10
an hour over here).

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk




----- Original Message -----
X-from: {Judith_A_Ruiz-at-whirlpool.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Monday, September 19, 2005 11:31 PM

Judith,
One question to ask your boss is, "How much do we use chemical information
(i.e. x-ray data) in our analyses?" Confocals don't generate x-ray data.
I'm not familiar with them and others may have better information, but is
there any chemical data generated? In my experience materials and forensic
work is often more concerned with the chemistry than the images.

Ken Converse

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: keith.morris-at-ucl.ac.uk [mailto:keith.morris-at-ucl.ac.uk]
Sent: Wednesday, September 21, 2005 5:13 AM
To: kenconverse-at-qualityimages.biz

Dear Judith,

I was under the impression that all that an SEM was really good for was for
the evaluation of the surface of non-living materials (unless you are into
vaporising bunnies). Confocal might be OK for polymer coatings, but SEM
images are rather in a different league to confocal in terms of resolution
and
clarity. There is an article on polymers at
http://fire.nist.gov/bfrlpubs/build04/art034.html .

I have occasionally viewed things under confocal that I am used to seeing
under
SEM or even under LM phase contrast with a CCD camera, like recovered
inhaled mineral fibres, pollen grains, or Nuclepore filter surfaces, and
thought wow they look really bad. We only use laser confocals here because
our delicate water based life forms aren't very happy in a vacuum (and to be
honest the confocal does come alive with intra-cellular fluorescent
markers).
In my last days at Harwell 10 years ago a new Camscan SEM was a revelation
with digital image VDU, PC control and Quantimet style image analysis,
compared to our older SEM machines (picture quality was pretty similar
though).

I'm sure the manufacturers would be very keen to demo a confocal at their
head
office (our confocals are designed for living cells). I would have thought
you
could rent time on a local confocal if it is needed occasionally (we charge
£10
an hour over here).

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk




----- Original Message -----
X-from: {Judith_A_Ruiz-at-whirlpool.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Monday, September 19, 2005 11:31 PM

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (icmicroanalysis-at-cox.net) from http://www.microscopy.com/MLFormMail.html on Wednesday, September 21, 2005 at 01:27:31
---------------------------------------------------------------------------

Email: icmicroanalysis-at-cox.net
Name: Dave

Title-Subject: [Filtered] Leveling platform for microscope

Question: I'm looking for a relatively inexpensive mechanical leveling platform that can be put onto a standard microscope stage. I want to be able to level a flat sample for imaging across both x and y directions. Any ideas on sources for the above would be approciated.

---------------------------------------------------------------------------

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From: dsherman-at-purdue.edu
Date: Wed, 21 Sep 2005 10:27:42 -0500
Subject: [Microscopy] SEM- blood cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers:

We used to prepare blood cells using standard fixation and dehydration
but then transfer into a Freon. Once in the freon you could just put a
droplet of the sample solution on a nucleopore filter. The freon would
evaporate instantly leaving lovely dried cells.

We tried this recently with the remains of freon we have had around for
years. It did not work well so I am assuming that the freon absorbed water
over the years and is not longer usable.

I do not know that type of freon this was as I inherited it. Most types are
no longer available. Does anyone know of a type that is still available and
can be used for this purpose? Any other hints for processing blood cells?
We can do standard CPD but this other method was soooo nice and easy with
such good results when it worked.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



==============================Original Headers==============================
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From: nash-at-molbio.uoregon.edu
Date: Wed, 21 Sep 2005 10:49:44 -0500
Subject: [Microscopy] GFP scope for green worm instruction.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,
I am hoping to set up a scope to look at gfp-expressing worms within the
context of workshops aimed at small groups high school students.I am
hoping to find something that will give reasonably good views of worms and
video output for demonstrations without the expense of a full-blown
research scope. Oh yeah, it would be great if it were tough, too. Has
anyone had any experience with a similar set of goals? Any suggestions?
Thanks very much,
Bruce Nash
nash-at-molbio.uoregon.edu

==============================Original Headers==============================
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From: paul_hazelton-at-umanitoba.ca
Date: Wed, 21 Sep 2005 10:54:22 -0500
Subject: [Microscopy] Re: SEM- blood cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Amoz,

Have a look at the QX-5 computer microscope by Digital Blue - its great fun
and puts the image on a PC screen. I knew it as the Intel QX-3. Its cheap at
$60 and can even be modified to include an Abbe condenser to considerably
improve images (see http://micro.magnet.fsu.edu/optics/intelplay/index.html
(the QX-5 is basically the same microscope as the discontinued Intel QX-3
but updated). I assume 7th graders are around 11-12 in age (year 7 in the
UK). Once on the PC the 640x480 images can be manipulated and pasted
etc, and it does time-lapse for living plants growing and small animals.

Website for the QX-5 http://www.playdigitalblue.com/products/qx5/info/.
Every school in the UK was given one of these in 2002. I've seen it for sale
at http://www.toygroove.com/qx5-computer-microscope.html . It's not got
the resolution of even a standard 'school' compound microscope though.
The QX-5 is also a bit more delicate for unsupervised boys so may be
better suited to teacher lead demos.

If you want a 'real' microscopes have a look at companies like Meade who
make a variety of microscopes from $70 to $700
http://www.meade.com/readiView/. They will all show simple things like
cells with stained nuclei, but naturally the more you pay the better
the view (and the more expensive ones come with an internal light source).
4x, 10x & 40x is fine for seeing cells (with a 8x eyepiece that's around 30x
to 400x magnification), any 100x objectives are fairly useless at these
lower prices ). No doubt other schools and colleagues can advise on brands.
Our research microscopes cost nearer £200,000.

Excellent pre-prepared stained slides of plant stems and leaves
or bits of rats, insects etc.. can be bought, but they tend to be expensive
and are easily broken. Mounted slides keep well so 'vintage' ones even from
50 years ago can still look OK.

Have a look at ebay.com for student microscopes or slides as they will often
be cheaper second-hand (e.g. Bausch & Lomb) or on offer (Meade sell
their via their factory outlet). Probably best to avoid children's toy
compound
or unbranded microscopes, although you can see something down them and
they are very cheap second-hand. Watch out for the delivery charge and check
their feedback on ebay. Old microscopes may need careful cleaning (very
soft tissues).

Generally carry the microscope by its back (something that caught
me out when teaching - I hadn't carried a research microscope for years as
they are bigger than a desk).

Also Google search the net for best prices and reviews (sometimes lab
supplies like slides are expensive on ebay). Unlike the QX-5 you
will need 1 compound microscope per pair or small group or bored queue.
You can still get one QX-5 as well for teacher demos.

By the way do try growing crystals on a slide, a few drops of a saturated
solution of salt (NaCl) or copper sulphate will grow superb crystals on the
surface of a slide when viewed under a microscope (but it takes a few hours
for the crystals to form and they often look best before the liquids all
gone). Make sure they don't drive the objective tips into the solution.
It's not biology but its fun.

Being in teaching myself a few years ago I didn't find any ready source of
free donated compound microscopes in the UK. Being in a poorer area our
parent association generated nothing either. However our village Playgroup
(kindergarten 2-5 years old) did quite well by sending begging letters and
charity grant applications far and wide, so give that a try.

Hope this is of some use.

Regards

Keith

PS. I submitted this email twice as the spam filter on the microscopy
listerver rejected it
----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk

----- Original Message -----
X-from: {aeckerson-at-deltacollegeprep.org}
To: {keith.morris-at-ucl.ac.uk}
Sent: Wednesday, September 21, 2005 12:53 AM

debby

try Vertrel. this is an 'environmentally friendly Freon replacement'
brought to us by Dow, the same people who gave us - you guessed it -
Freon. all considered, we can only assume it is environmentally
friendly in that there is currently no evidence of damage which it can
cause.

having said that last sentence, i use it inplace of freon for cleaning
scope parts and for purifying virus for different uses. it is almost as
expensive as freon, but i think you can get it in 1L bottles (we use
enought that we usually buy 4L bottles).

what is a reply that does not flog our own papers - for a reference see:
Mendez, Hermann, Hazelton and Coombs. 2000. A comparative analysis of
Freon substitutes in the purification of reovirus and calicivirus.
Journal of Virological Methods, 90:59-67. it is available as a free
paper in .pdf form from the journals division of Virus International, a
section Elsevier has.

but i have no stock in Dow or Elsevier, nor interest in use of Vertrel
other than our paper, and references look good in the citation indexes,
not?

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926



==============================Original Headers==============================
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From: David.Patton-at-uwe.ac.uk
Date: Wed, 21 Sep 2005 11:49:14 -0500
Subject: [Microscopy] SEM- blood cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We used HMDS recently. Lots of echinocytes but that is probably
unrelated.

Dave

-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: 21 September 2005 16:33
To: David Patton

Listers:

We used to prepare blood cells using standard fixation and
dehydration
but then transfer into a Freon. Once in the freon you could just put a
droplet of the sample solution on a nucleopore filter. The freon would
evaporate instantly leaving lovely dried cells.

We tried this recently with the remains of freon we have had around for
years. It did not work well so I am assuming that the freon absorbed
water
over the years and is not longer usable.

I do not know that type of freon this was as I inherited it. Most types
are
no longer available. Does anyone know of a type that is still available
and
can be used for this purpose? Any other hints for processing blood
cells?
We can do standard CPD but this other method was soooo nice and easy
with
such good results when it worked.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy



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From: tivol-at-caltech.edu
Date: Wed, 21 Sep 2005 13:19:20 -0500
Subject: [Microscopy] Philips EM430

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,
A colleague of mine asked me to post this:

Will you please forward this ad:

The equipment is:
Model: Philips Transmission Electron Microscope EM430
Serial #: D614

The machine has not been in service for the past five years. It comes
with full set of manuals. It is offered as is, without sample holders
for free, but the receiving party is responsible for relocating it.
Caltech requires the receiving party to sign a liability release on the
materials themselves. We only engage with parties who are prepared
with a rigger (professional person) and a big enough truck. This
professional should know in advance the exact model.

Thanks.

Hong

Hongxing Tang, Ph.D.
Senior Research Scientist
California Institute of Technology 114-36
Pasadena CA 91125
Tel: 626-395-2932 Fax: 626-683-9060

Please contact him directly at the address or phone numbers listed
above or at htang-at-caltech.edu.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: paul_hazelton-at-umanitoba.ca
Date: Wed, 21 Sep 2005 13:52:16 -0500
Subject: [Microscopy] correction of manufacturer-Vertrel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

sorry listers, sometimes the fingers move faster than the brain. both
Freon and Vertrel are products of du Pont, not Dow, as i previously
stated. i did know better.

should also point out that there are other environmentally friendly
replacements for Freon. i think most of our EM suppliers can give a
line on other products. i would check with SPI on that because i know
they can recommend alternatives.

no, no interest in du Pont, or SPI either.

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926


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From: carlos_micra-at-prodigy.net.mx
Date: Wed, 21 Sep 2005 17:55:45 -0500
Subject: [Microscopy] viaWWW: ISI service manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (carlos_micra-at-prodigy.net.mx) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 21, 2005 at 14:41:39
---------------------------------------------------------------------------

Email: carlos_micra-at-prodigy.net.mx
Name: C. Segovia

Organization: Micra

Title-Subject: [Filtered] MListserver:ISI service manual

Question: Hello everyone
Last month we get as a ìgiftî a SEM, an ISI model DS-130.
As far as the last user say it was in working condition when they use it for the last time 5 years ago.
We are going to install it but we would like to have a copy of the service manual of this instrument.
As far as I know since several years ago ISI is out of business, so I would like to know if some one of this list has the service manual for this particular instrument.
If some one has it please contact me off line to arrange the way to have a copy of it.
Thank you in advance for your help
CS
Mexico


---------------------------------------------------------------------------


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From: danon-at-cnea.gov.ar
Date: Wed, 21 Sep 2005 18:19:51 -0500
Subject: [Microscopy] A question on DTSA spectrum analyzer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I am new in the listserver and I have tried to search the archives before
posting my question, but I have found no clues so far.
I am trying to analyze EDS spectra taken from a Phillips CM-200 TEM with an
EDAX-DX4 system. I would like to use the DTSA spectrum analyzer; but the
data format of the DX4 system seems not to be suitable to be imported to
DTSA.
Does anybody happen to know if there is any free utility to convert .spc
files to the EMSA or MSA data format?
Any advise will be greatly appreciated.

Regards,

Ariel Danon
Materials Department
National Commission of Atomic Energy
Buenos Aires, Argentina


==============================Original Headers==============================
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From: wamann2-at-metalmat.ufrj.br
Date: Thu, 22 Sep 2005 10:33:12 -0500
Subject: [Microscopy] BibMic searchable database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Brandi,

I don't know if any microscopists answered your question (none were
posted) so I'll reply.

Generally to use these very expensive microscopes you need a good biology
degree (cell & molecular biology). Most microscope 'users' will have then
gone on to get a PhD (doctorate) in cell or tissue biology (working with
animals, plants and/or bacteria & fungi). Its then that they may start to
use these types of microscopes a lot, as fluorescent markers are used to
trace intra-cellular biological processes. So it takes a lot of work to get
to be a regular user. The only downside is that even with a PhD in biology
you may not succeed in research as it is quite competitive. It helps if your
PhD tutor is well known, and the research is in a popular area like how to
stop cells becoming cancers (or curing blindness in our institutes case).
I'm sure your Biology department at your university can advise further.

There aren't many openings for non graduates, but sometimes research
organisations will take on technical support staff with good school exam
results (normally aged 18-20) and train them in things like this. In the UK
such staff will often be expected to take a part-time biology degree or
similar qualification while working (paid for by the organisation).

You can learn a lot about research microscopes at http://www.microscopyu.com

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk

----- Original Message -----
X-from: {mrsquinlin-at-gmail.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Monday, September 19, 2005 11:43 PM

Fellow microscopists
Allow me to offer you the use of

BIBMIC - A Bibliography of Books Relating to Materials Microscopy
which is a searchable database of books on Materials Microscopy.
This was previously published on paper in
Metallography 22(1989)123-176 (518 references),
and Materials Characterization 36(1996)105-149 (975 references),
and first offered on the Internet in 2000.

It has been upgraded (February 2005) to 1170 references.

It is sited at (bookmark!)
http://bibmic.metalmat.ufrj.br

I welcome any additions you might suggest, please email me at
wamann-at-metalmat.ufrj.br

Hope it is useful, greetings to all from Brazil


Dr.Walter A.Mannheimer
Professor Emeritus
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505 21945 Rio de Janeiro, Brazil
Voice +5521 2562-8500 (Dept.office) +5521 2562-8517 (direct)
Fax +5521 2290-6626 Email: wamann-at-metalmat.ufrj.br
http://www.metalmat.ufrj.br/hpwamann


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9, 21 -- Subject: BibMic searchable database
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From: wesaia-at-iastate.edu
Date: Thu, 22 Sep 2005 11:28:44 -0500
Subject: [Microscopy] SEM roughness measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would think that difference in resolution may be due to the nature of the
measurement.

If we can only make measurements as fine as D in the image, I would think
our error in the Z direction would be D/Sine(theta) or about 11D for 5
degrees of tilt (sine(5)=0.087)

Warren

At 11:37 AM 09/18/05, donc-at-asmicro.com wrote:

} Mike Bode described a way of estimating the vertical resolution that can be
} achieved by using stereo pairs. His trigonometric calculation predicts that
} the practical vertical resolution is 10x worse than the lateral resolution,
} for tilt angles of 6-10 degrees. I wonder whether users of various SEM
} measurement tools have the same experience in actual practice. And I wonder
} what the observed limits of vertical resolution are for the highest
} resolution FE-SEMs.
} regards,
} Don Chernoff
} ==================================
} Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
} 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
} INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
} web: http://www.asmicro.com Fax: 317-895-5652
} [business activities: analytical services in AFM, AFM probes, consulting,
} training,
} calibration and test specimens, calibration and measurement software,
} used NanoScope equipment.]
}
} ----- Original Message -----
} From: Mike.Bode-at-soft-imaging.net
} To: donc-at-asmicro.com
} Sent: Friday, September 16, 2005 6:34 PM
} Subject: [a] [Microscopy] RE: SEM roughness measurement
}
}
}
} Hello Daniel,
}
} Yes, you can use stereo pairs to calculate a surface profile and from
} there calculate roughness parameters. We have a module for our analySIS
} software that accomplishes this. If you want more information, please
} contact me by email.
}
} There are certain limitations to what you can do with this technique. The
} z-resolution depends on a number of factors, the most important of which is
} the tilt angle. For typical tilt angles of 6 - 10 degrees, the resolution is
} roughly 1/10th of the lateral resolution (you can calculate that by
} multiplying the lateral resolution with the tan of the stereo angle). For
} example: If your stereo images have a resolution of 1 micron (1mm x 1mm
} field of view and an image resolution of 1000 x 1000 pixels), your
} z-resolution will be on the order of 10 microns. In order to evaluate if the
} technique will do what you want, you need the following information:
} required field of view, image resolution, stereo angle.
}
} Let me know if you need further info.
}
} mike
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 12596 West Bayaud Avenue
} Suite 300
} Lakewood, CO 80228
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
} -----Original Message-----
} X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca
} [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca]
} Sent: Friday, September 16, 2005 4:53 PM
} To: Mike Bode
} Subject: [Microscopy] SEM roughness measurement
}
} Hi everyone,
}
} Does anyone have experience with deriving roughness information from SEM
} images? I presume you need to use stereo pairs to get 3D information from a
} rough substrate.
}
} I would be especially interested if anyone had a free/cheap software
} package that would handle this task.
}
} Thanks,
} Daniel Salamon
} Technical Officer, Electron Microscopy
}
} National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116
} Street Edmonton, AB. T6G 2V4
}
} Phone: Office (780) 492 8878
} Lab (780) 492 8872
} DocuFax: (780) 492 8632


==============================Original Headers==============================
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6, 21 -- To: MSA listserver {Microscopy-at-msa.microscopy.com}
6, 21 -- From: Warren E Straszheim {wesaia-at-iastate.edu}
6, 21 -- Subject: Re: [Microscopy] RE: SEM roughness measurement
6, 21 -- Cc: donc-at-asmicro.com
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From: DusevichV-at-umkc.edu
Date: Thu, 22 Sep 2005 16:31:26 -0500
Subject: [Microscopy] RE: SEM roughness measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} I would think that difference in resolution may be due to the
} nature of the
} measurement.
}
} If we can only make measurements as fine as D in the image, I
} would think
} our error in the Z direction would be D/Sine(theta) or about
} 11D for 5
} degrees of tilt (sine(5)=0.087)
}
} Warren

I think we are talking really about measurement error, not about
resolution.

If dP - error of parallax measurements (x direction), then
dZ = dP/2*sin(theta/2), if we know exact tilt angle (theta error is
zero).
If theta=10, then dZ=5.7*dP. For relatively flat surfaces we can use
tilt angle equal to 20 degrees and even higher. For theta=20 dZ=2.9*dP.
Not too bad, since dP usually is smaller than 1% of the field of view.

Error in theta measurements gives another component of dZ. Unfortunately
some microscopes does not equipped for good theta measurements and
for them theta error could be as high as 1 degree. In this case dZ is
about 10% of the measured value of z (at theta=10). For microscopes with
a good
goniometer this part of error is pretty small and in any case can
be minimized by increasing theta.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy



} At 11:37 AM 09/18/05, donc-at-asmicro.com wrote:
}
} } Mike Bode described a way of estimating the vertical resolution that
} } can be achieved by using stereo pairs. His trigonometric
} calculation
} } predicts that the practical vertical resolution is 10x worse
} than the
} } lateral resolution, for tilt angles of 6-10 degrees. I
} wonder whether
} } users of various SEM measurement tools have the same experience in
} } actual practice. And I wonder what the observed limits of vertical
} } resolution are for the highest resolution FE-SEMs. regards,
} } Don Chernoff
} } ==================================
} } Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
} } 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
} } INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in
} USA & Canada)
} } web: http://www.asmicro.com Fax: 317-895-5652
} } [business activities: analytical services in AFM, AFM
} probes, consulting,
} } training,
} } calibration and test specimens, calibration and measurement software,
} } used NanoScope equipment.]
} }
} } ----- Original Message -----
} } From: Mike.Bode-at-soft-imaging.net
} } To: donc-at-asmicro.com
} } Sent: Friday, September 16, 2005 6:34 PM
} } Subject: [a] [Microscopy] RE: SEM roughness measurement
} }
} }
} }
} } Hello Daniel,
} }
} } Yes, you can use stereo pairs to calculate a surface profile and
} } from there calculate roughness parameters. We have a module for our
} } analySIS software that accomplishes this. If you want more
} information,
} } please contact me by email.
} }
} } There are certain limitations to what you can do with this
} } technique. The z-resolution depends on a number of factors, the most
} } important of which is the tilt angle. For typical tilt
} angles of 6 - 10
} } degrees, the resolution is roughly 1/10th of the lateral resolution
} } (you can calculate that by multiplying the lateral
} resolution with the
} } tan of the stereo angle). For
} } example: If your stereo images have a resolution of 1 micron
} (1mm x 1mm
} } field of view and an image resolution of 1000 x 1000 pixels), your
} } z-resolution will be on the order of 10 microns. In order to
} evaluate if the
} } technique will do what you want, you need the following information:
} } required field of view, image resolution, stereo angle.
} }
} } Let me know if you need further info.
} }
} } mike
} }
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 12596 West Bayaud Avenue
} } Suite 300
} } Lakewood, CO 80228
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} } -----Original Message-----
} } X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca
} } [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca]
} } Sent: Friday, September 16, 2005 4:53 PM
} } To: Mike Bode
} } Subject: [Microscopy] SEM roughness measurement
} }
} } Hi everyone,
} }
} } Does anyone have experience with deriving roughness
} information from
} } SEM images? I presume you need to use stereo pairs to get 3D
} } information from a rough substrate.
} }
} } I would be especially interested if anyone had a
} free/cheap software
} } package that would handle this task.
} }
} } Thanks,
} } Daniel Salamon
} } Technical Officer, Electron Microscopy
} }
} } National Institute for Nanotechnology, NRC W6-017A ECERF Bldg,
} } 9107-116 Street Edmonton, AB. T6G 2V4
} }
} } Phone: Office (780) 492 8878
} } Lab (780) 492 8872
} } DocuFax: (780) 492 8632
}
}
} ==============================Original
} Headers==============================
} 6, 21 -- From wesaia-at-iastate.edu Thu Sep 22 11:28:44 2005
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} (mailhub-3.iastate.edu [129.186.140.13])
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} ESMTP id j8MGShos013801
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} Sep 2005 11:28:44 -0500
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From: jacqui.ross-at-auckland.ac.nz
Date: Fri, 23 Sep 2005 07:29:20 -0500
Subject: [Microscopy] viaWWW: Academic Position in Biomedical Imaging: New Zealand

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jacqui.ross-at-auckland.ac.nz) from http://www.microscopy.com/MLFormMail.html on Friday, September 23, 2005 at 01:58:51
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Email: jacqui.ross-at-auckland.ac.nz
Name: Jacqueline Ross

Organization: The University of Auckland

Title-Subject: [Filtered] Academic Position in Biomedical Imaging: New Zealand

Question: The Department of Anatomy with Radiology in the School of Medical Sciences invites applications for the post of Senior Lecturer (equivalent to Associate Professor in North America) to assume the Directorship of the Biomedical Imaging Research Unit and to teach in the area of biomedical imaging and cell and tissue biology.

The successful applicant will hold a PhD and be someone who has research interests in the areas of cell and tissue biology and imaging and who can provide evidence of success with national and international research funding.

Equipment in the Biomedical Imaging Research Unit currently includes: 1 transmission electron microscope and 2 confocal laser scanning microscopes. There are also three brightfield/fluorescence microscope systems with digital cameras within the BIRU, two of which have live cell imaging capabilities. Image analysis and 3D volume rendering software packages are also available. Please refer to the BIRU website http://www.health.auckland.ac.nz/biru/ for further details.

Three dedicated staff are based in the BIRU to provide research support and training.. The Directorís role includes determining areas of growth within the imaging unit and new equipment and technology purchases.

For further information and to apply online please visit
www.vacancies.auckland.ac.nz.

Please quote Vacancy Number A515-05. Applications close 7 October 2005. Further information may be obtained by emailing the Head of Department, Associate Professor Cynthia Jensen at:
c.jensen-at-auckland.ac.nz.


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From: nicholas.ritchie-at-nist.gov
Date: Fri, 23 Sep 2005 10:26:54 -0500
Subject: [Microscopy] NIST/MAS Particle Workshop 2006

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------------------------------------
NIST/MAS Particle Workshop 2006
-------------------------------------

The ongoing series of topical workshops sponsored by the National
Institute of Standards and Technology and the Microbeam Analysis Society
will continue at NIST’s Gaithersburg campus the 24^th through 26^th of
April 2006. This workshop will focus on microscopic techniques for
analyzing particles from millimeter to nanometer size range. The
techniques discussed will include SEM/EDS, AEM, TOF/SIMS, optical, FIB
and scanned probe microscopies. The workshop format will bring together
industrial and government laboratory users with leading researchers.
Many different industries will be represented including the
pharmaceutical, mining, environmental, semiconductor, space science,
nanomaterials, forensics and manufacturing industries. The focus will be
on discussing current usage and the current state-of-the-art and
identifying productive demand-driven avenues for future research. There
is no registration fee however attendance is limited to the first 300
registrants. Additional information and an online registration form is
available at http://www.nist.gov/particle. For answers to questions that
are not addressed by the web site please contact Nicholas Ritchie
(nicholas.ritchie-at-nist.gov).



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From: ahlst007-at-umn.edu
Date: Fri, 23 Sep 2005 12:16:29 -0500
Subject: [Microscopy] Re: A question on DTSA spectrum analyzer

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Listers,

I've been wondering the same thing as Ariel Danon. I have the EDAX Power MX
system, a Macintosh computer based system - a very rare bird, indeed -
mounted on my FEI CM-12 TEM, and the quantitation has developed some bugs
over the years (software version 1.00). Like Ariel, I cannot get DTSA to
take in the EDAX format .spc either. I've been told that if I could save the
spectrum as an EXCEL spreadsheet, I could import that into DTSA, but my
software version 1.00 apparently will not allow me to do that.

Could someone familiar with DTSA and its import function perhaps give Ariel
and I some ideas on how to get .spc into DTSA?

Gib
--
Gib Ahlstrand, Scientist
Electron Optical Facility, University of Minnesota, CBS Imaging Center,
35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
(612)624-2785 FAX, ahlst007-at-tc.umn.edu
http://www.cbs.umn.edu/ic/

} Hi all,
}
} I am new in the listserver and I have tried to search the archives before
} posting my question, but I have found no clues so far.
} I am trying to analyze EDS spectra taken from a Phillips CM-200 TEM with an
} EDAX-DX4 system. I would like to use the DTSA spectrum analyzer; but the
} data format of the DX4 system seems not to be suitable to be imported to
} DTSA.
} Does anybody happen to know if there is any free utility to convert .spc
} files to the EMSA or MSA data format?
} Any advise will be greatly appreciated.
}
} Regards,
}
} Ariel Danon
} Materials Department
} National Commission of Atomic Energy
} Buenos Aires, Argentina



==============================Original Headers==============================
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7, 17 -- Subject: Re: A question on DTSA spectrum analyzer
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From: wesaia-at-iastate.edu
Date: Fri, 23 Sep 2005 13:07:50 -0500
Subject: [Microscopy] A question on DTSA spectrum analyzer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A fellow by the name of David Vowles (djv23-at-cam.ac.uk) wrote a program
called Spectrum Plot that reads and converts many formats of EDS files. It
lists EDAX as one of the supported formats. I took an interest because of
its support for Link ISIS format and for batch file conversion. If your
EDAX saves files with an extension of .SPC there may be hope.

I got a copy of the software on CD from David. I don't know if he has an
on-line copy of it. I don't think he would have an objection to me
forwarding my copy, but you should probably check with him first.

Warren

At 12:17 PM 09/23/05, you wrote:

} Listers,
}
} I've been wondering the same thing as Ariel Danon. I have the EDAX Power MX
} system, a Macintosh computer based system - a very rare bird, indeed -
} mounted on my FEI CM-12 TEM, and the quantitation has developed some bugs
} over the years (software version 1.00). Like Ariel, I cannot get DTSA to
} take in the EDAX format .spc either. I've been told that if I could save the
} spectrum as an EXCEL spreadsheet, I could import that into DTSA, but my
} software version 1.00 apparently will not allow me to do that.
}
} Could someone familiar with DTSA and its import function perhaps give Ariel
} and I some ideas on how to get .spc into DTSA?
}
} Gib
} --
} Gib Ahlstrand, Scientist
} Electron Optical Facility, University of Minnesota, CBS Imaging Center,
} 35 Snyder Hall, St. Paul, MN. USA. 55108 (612)624-3454
} (612)624-2785 FAX, ahlst007-at-tc.umn.edu
} http://www.cbs.umn.edu/ic/
}
} } Hi all,
} }
} } I am new in the listserver and I have tried to search the archives before
} } posting my question, but I have found no clues so far.
} } I am trying to analyze EDS spectra taken from a Phillips CM-200 TEM with an
} } EDAX-DX4 system. I would like to use the DTSA spectrum analyzer; but the
} } data format of the DX4 system seems not to be suitable to be imported to
} } DTSA.
} } Does anybody happen to know if there is any free utility to convert .spc
} } files to the EMSA or MSA data format?
} } Any advise will be greatly appreciated.
} }
} } Regards,
} }
} } Ariel Danon
} } Materials Department
} } National Commission of Atomic Energy
} } Buenos Aires, Argentina


==============================Original Headers==============================
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From: xyang-at-SMU.CA
Date: Fri, 23 Sep 2005 13:26:00 -0500
Subject: [Microscopy] EOL JEM-100C vacuum circuit

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I am seeking a clear circuit diagram of vacuum board for our JEOL JEM-100C
TEM system. We are running into a vacuum trouble because the diffusion pumps
did not work. We could not read any numbers from the existing diagram we
have in the lab. The number of the diagram is E-2-14.

This is a old old machine I have to say. Any help would be appreciated.

Best Regards,
----------------------------------------------
Xiang Yang
Electron Microscopy Center
Faculty of Graduate Studies and Research
Saint Mary's University
Science Building, Suite 007 and 101
923 Robie Street
Halifax, NS Canada B3H 3C3
Tel: (902) 496-8292 (lab)
(902) 420-5709 (off)
Email: xiang.yang-at-smu.ca


==============================Original Headers==============================
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From: cbalane-at-wesleyan.edu
Date: Sat, 24 Sep 2005 17:38:40 -0500
Subject: [Microscopy] latex beads for microscopy

Contents Retrieved from Microscopy Listserver Archives
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Hi everyone,

I'd like to use FITC-conjugated 6 nanometer beads for several cytology
projects that will be subjected to confocal micrography. I checked with
several companies but they don't seem to have the 6 nanometer size that I'd
like to use in my experiment. Does anyone know of a company that sells them?



Thanks,
Carlo



--
Carlo Franco Bolivar Balane

Box 4058, 222 Church Street, or Wolfe Laboratory
Wesleyan University Station Rm. 157, HA Laboratories
Middletown, CT, 06459-4058 Wesleyan University
phone: 1.860.759.2830 phone: 1.860.685.3275


==============================Original Headers==============================
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10, 32 -- Subject: latex beads for microscopy
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From: fundatel-at-gmail.com
Date: Sun, 25 Sep 2005 08:17:20 -0500
Subject: [Microscopy] viaWWW: looking SEM and TEM Surplus Donations

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (fundatel-at-gmail.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, September 23, 2005 at 16:59:08
---------------------------------------------------------------------------

Email: fundatel-at-gmail.com
Name: Fernando Balducci

Organization: FUNDATEL

Title-Subject: [Filtered] looking SEM and TEM to receive it in Donation

Question: Hello alls
FUNDATEL, non profit organization located in Argentina, is looking for a working TEM and SEM and any other equipment to received it in Donation.
we will use the equipment to offer education/training and to perform R&D project in our region of influence

We also pay the costs of shipping and handling...
Please contact via email to
fundatel-at-gmail.com
subject: donation

thanks in advance

Fernando Balducci
President
FUNDATEL

---------------------------------------------------------------------------

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From: kevin.braeckmans-at-ugent.be
Date: Mon, 26 Sep 2005 07:12:48 -0500
Subject: [Microscopy] RE: latex beads for microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well known beads companies are

Spherotech (www.spherotech.com)
Duke Scientific (www.dukescientific.com)
Bangs Laboratories (www.bangslabs.com)

I don't know if they have FITC conjugated beads, but definitely beads
(including 6 micron) with fluorescent dyes with a comparable
excitation/emission spectrum (and much more photostable than FITC).

Good luck and best regards,

Kevin



Kevin Braeckmans, Ph.D.
Lab. General Biochemistry & Physical Pharmacy
Ghent University
Harelbekestraat 72
9000 Ghent
Belgium
Tel: +32 (0)9 264.80.78
Fax: +32 (0)9 264.81.89
E-mail: Kevin.Braeckmans-at-UGent.be


} -----Oorspronkelijk bericht-----
} Van: cbalane-at-wesleyan.edu [mailto:cbalane-at-wesleyan.edu]
} Verzonden: zondag 25 september 2005 0:47
} Aan: kevin.braeckmans-at-ugent.be
} Onderwerp: [Microscopy] latex beads for microscopy
}
}
}
}
} --------------------------------------------------------------
} --------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} --------------
}
} Hi everyone,
}
} I'd like to use FITC-conjugated 6 nanometer beads for several
} cytology projects that will be subjected to confocal
} micrography. I checked with several companies but they don't
} seem to have the 6 nanometer size that I'd like to use in my
} experiment. Does anyone know of a company that sells them?
}
}
}
} Thanks,
} Carlo
}
}
}
} --
} Carlo Franco Bolivar Balane
}
} Box 4058, 222 Church Street, or Wolfe Laboratory
} Wesleyan University Station Rm. 157, HA Laboratories
} Middletown, CT, 06459-4058 Wesleyan University
} phone: 1.860.759.2830 phone: 1.860.685.3275
}
}
} ==============================Original
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From: mckee-at-HELIX.MGH.HARVARD.EDU
Date: Mon, 26 Sep 2005 07:48:09 -0500
Subject: [Microscopy] tabletop print processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Carlo,

I tend to buy fluorescent beads from Polyscience or Molecular probes. In my
old days at Harwell we used to make our own particles (often radioactive
fused aluminosilicate clay particles [FAP] as in-vivo radio-tracers rather
than fluorescent though). 0.006 um is rather small though, as most
manufactured 'standard' sizes of particles seem to only go down to around
20nm, e.g. see http://www.dukescientific.com. Personally I've never used a
size of fluorescent particles below 0.04 um (and even these naturally behave
more like a stain, being too small to resolve optically).

However Polyscience and Molecular probes both have a bespoke service
for custom microparticle production and should be able to help for a price:

"Our Polybead® polystyrene particles are excellent for a variety of
applications because they are monodisperse. In addition to the standard
products contained in our catalog, we can synthesize materials designed to
your specifications. If you require a custom microparticle preparation,
please contact us for a quote. "

Link: http://www.polysciences.com/shop/overview.asp?oid=2

See also Molecular probes:

"We can tailor-make colored and unstained microspheres of many sizes,
surface chemistries, densities and volumes to meet the diverse needs of
customers, including academic, industrial and government laboratories, as
well as major global diagnostic companies; please contact our Custom and
Bulk Sales Department for more information."

Link: http://probes.invitrogen.com/handbook/sections/0605.html

It might be useful to have an idea of price for these bespoke particles if
you have or get one.

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk
----- Original Message -----
X-from: {cbalane-at-wesleyan.edu}
To: {keith.morris-at-ucl.ac.uk}
Sent: Saturday, September 24, 2005 11:46 PM

Good morning, List,

I'm looking for recommendations for a tabletop B/W print processor (Like the
Ilford 2150 RC) for a new darkroom. (Yes, we have digital, but I still want
to be able to use film). Thanks.

Mary McKee
Program in Membrane Biology
Massachusetts General Hospital
185 Cambridge St.
Boston, MA 02114

(617)726-3696
--



==============================Original Headers==============================
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6, 16 -- Date: Mon, 26 Sep 2005 08:19:32 -0700
6, 16 -- Subject: tabletop print processors
6, 16 -- From: Mary McKee {mckee-at-HELIX.MGH.HARVARD.EDU}
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From: mcauliff-at-umdnj.edu
Date: Mon, 26 Sep 2005 08:32:17 -0500
Subject: [Microscopy] Re: tabletop print processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mary:

I don't know if such a beast exists anymore. Did you know that
Ilford is in receivership and that Kodak will stop making B&W paper at
the end of the year? I love film, real film. Most of my many cameras are
'antiques' by today's standards. I have books on photochemistry and I
mix all of my developers (film and paper) from scratch. I would just buy
a good printer for B&W work and a film scanner.

Geoff

mckee-at-HELIX.MGH.HARVARD.EDU wrote:

} Good morning, List,
}
} I'm looking for recommendations for a tabletop B/W print processor (Like the
} Ilford 2150 RC) for a new darkroom. (Yes, we have digital, but I still want
} to be able to use film). Thanks.
}
} Mary McKee
} Program in Membrane Biology
} Massachusetts General Hospital
} 185 Cambridge St.
} Boston, MA 02114
}
} (617)726-3696
}
}


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: hyi-at-emory.edu
Date: Mon, 26 Sep 2005 11:09:37 -0500
Subject: [Microscopy] (Microscopy) Film scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Everyone:

           I am about to purchase a Nikon Cool Scan 9000 film
scanner. Does anyone out there have experience with this scanner. Would
you tell me if you like it or not? Recommendations on other models are
welcome too. Thank you in advance.

Hong Yi
Emory School of Medicine EM


==============================Original Headers==============================
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From: gary-at-gaugler.com
Date: Mon, 26 Sep 2005 12:10:40 -0500
Subject: [Microscopy] Re: (Microscopy) Film scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is the new replacement for the Coolscan 8000ED.
I'm not sure what Nikon did to improve an already
great scanner. If you want to scan TEM negs, they do
not directly fit. The sides need to be trimmed away
a bit and then use the 6x7cm holder. What media do
you intend to mostly scan? Unless you are doing 35mm,
the 8000/9000 at high rez will generate HUGE TIFF files.
If you back off on rez, then size goes down. But then,
you could buy some lesser rez scanner.

One of the big things the 8000/9000 have going for them
is very high Dmax (4.8). Lesser scanners typically do not
have this.

gary g.



At 09:12 AM 9/26/2005, you wrote:



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From: grb-at-ufl.edu
Date: Mon, 26 Sep 2005 15:45:14 -0500
Subject: [Microscopy] FIB GIS Platinum reservoir

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone have experience with refilling the platinum reservoir on a
FEI FIB GIS. I am interested in the procedure and the volume to refill.

--
Gerald Bourne
Major Analytical Instrumentation Center
Department of Materials Science and Engineering
University of Florida
107H MAEC
P.O. Box 116400
Gainesville, FL 32611
(352) 392-6985
(352) 392-0390 fax


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From: ptlozier-at-aol.com
Date: Mon, 26 Sep 2005 18:20:07 -0500
Subject: [Microscopy] AskAMicroscopist: Displaying Images to ClassRoom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ptlozier-at-aol.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, September 26, 2005 at 17:55:00
---------------------------------------------------------------------------

Email: ptlozier-at-aol.com
Name: Peter Lozier

Organization: Wilbour School

Education: 6-8th Grade Middle School

Location: Little Compton, RI

Question:
I have a fair ($600) stereo trinocular disecting microscope with a ccd color camera attachment. My question is: what is the best (under ~$200) video capture card with software that I can use to display images on a computer for class demonstrations. The camera output is "BNC" F and S video.

Thanks for your help.

Peter Lozier

---------------------------------------------------------------------------

==============================Original Headers==============================
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9, 12 -- From: ptlozier-at-aol.com (by way of Ask-A-Microscopist)
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From: dljones-at-bestweb.net
Date: Mon, 26 Sep 2005 18:42:08 -0500
Subject: [Microscopy] Re: AskAMicroscopist: Displaying Images to ClassRoom

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Peter,

I guess I don't understand why you can't just run the S video output to a
television monitor for the class to see. I would think your audio-visual
department would have a compatible television.

dj

On Mon, 26 Sep 2005 ptlozier-at-aol.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ptlozier-at-aol.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, September 26, 2005 at 17:55:00
} ---------------------------------------------------------------------------
}
} Email: ptlozier-at-aol.com
} Name: Peter Lozier
}
} Organization: Wilbour School
}
} Education: 6-8th Grade Middle School
}
} Location: Little Compton, RI
}
} Question: I have a fair ($600) stereo trinocular disecting microscope with a
} ccd color camera attachment. My question is: what is the best (under ~$200)
} video capture card with software that I can use to display images on a
} computer for class demonstrations. The camera output is "BNC" F and S video.
}
} Thanks for your help.
}
} Peter Lozier
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 9, 12 -- From zaluzec-at-ultra5.microscopy.com Mon Sep 26 18:20:07 2005
} 9, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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} 9, 12 -- From: ptlozier-at-aol.com (by way of Ask-A-Microscopist)
} 9, 12 -- Subject: AskAMicroscopist: Displaying Images to ClassRoom
} 9, 12 -- Content-Type: text/plain; charset="us-ascii"
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}


==============================Original Headers==============================
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From: marilena.re-at-brindisi.enea.it
Date: Tue, 27 Sep 2005 01:24:42 -0500
Subject: [Microscopy] Telecamera for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear mail listers,
I would like to get information about your experience to find a good
telecamera for a stereomicroscope. It should be possible to put it directly
on the binocular of the optical microscope. Have you used it and found a
suitable telecamera with a good quality but with this possibility?
What kind of telecamera?
Thanks in advance
Marilena Re
Marilena Re
ENEA - Materials and Technology
Composite and Nanostructured Materials Section
C.R. Brindisi
marilena.re-at-brindisi.enea.it
tel 0831-201444


==============================Original Headers==============================
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2, 23 -- From: "Marilena Re" {marilena.re-at-brindisi.enea.it}
2, 23 -- To: {Microscopy-at-microscopy.com}
2, 23 -- Subject: Telecamera for LM
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From: opto-at-klughammer.de
Date: Tue, 27 Sep 2005 03:25:23 -0500
Subject: [Microscopy] Re: GFP scope for green worm instruction.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Mr. Nash,

we could offer you the following system:

The Video ZFL Scope offered by klughammer bio gmbh, Germany is a
Macro/Micro fluorescent vision system that utilizes
interchangeable professional fluorescent cubes and internal focus to
create an image compatible with most existing camera
systems. It is a simple means of doing very sophisticated, task oriented
fluorescence without the expense and complexity
associated with a fully loaded research microscope.

Basic Components of the ZFL Video Fluorescent System are:

1. A light source emitting the wavelengths required to cause the
labeling dye to fluoresce.
Two different remote light sources are available, a halogen light for
the longer wavelengths, and a metal arc lamp for
the UV.

2. An integrated cube that optimizes performance by stopping all but the
desired (excitation) wavelength from
reaching the object and then stopping all but the fluorescing
wavelength (emitting) from reaching the camera. There is a
multitude of off-the-shelf cubes available depending on which
labeling dye is being used.

The system permits the usage of all standard Olympus BX2 Fluorescent
Cubes, which are available from multiple
sources. These are captured singularly in a quick change holder
requiring only a minute to interchange.

3. A camera whose sensitivity and bandwidth are adequate to handle the
fluorescing light levels (which can sometimes be
minimal).

4. Optical and mechanical parts

The ZFL system is suitable for operation in either Macro or Micro mode.

If you would like to receive more information, please contact us.

Anneliese Schmaus

klughammer bio gmbh
Strassbach 9
85229 Markt Indersdorf
Germany

Tel. +49 8136 6011
Fax +49 8136 7098

schmaus-at-klughammer.de
www.klughammer.de



nash-at-molbio.uoregon.edu schrieb:

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==============================Original Headers==============================
20, 20 -- From opto-at-klughammer.de Tue Sep 27 03:25:23 2005
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From: mckee-at-HELIX.MGH.HARVARD.EDU
Date: Tue, 27 Sep 2005 08:32:49 -0500
Subject: [Microscopy] EM facility user fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning again,

Thanks to all of you who responded to my post about B/W printers - I'm
digesting the info.

Here's another query. I know that this has been discussed before, but I
couldn't find the thread in the archives. We're re-evaluating what we
charge our users for EM services (embedding, sectioning, scope time, etc)
and would like to have an idea about what other academic institutions
charge. If anyone is willing to discuss this, would you please e-mail me
off-list or call me? Thanks.

Mary

Mary McKee
Program in Membrane Biology
Massachusetts General Hospital
Boston, MA 02114

(617)726-3696
--



==============================Original Headers==============================
8, 16 -- From MCKEE-at-HELIX.MGH.HARVARD.EDU Tue Sep 27 08:32:49 2005
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8, 16 -- Date: Tue, 27 Sep 2005 09:04:05 -0700
8, 16 -- Subject: EM facility user fees
8, 16 -- From: Mary McKee {mckee-at-HELIX.MGH.HARVARD.EDU}
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From: mcintyre-at-optics.rochester.edu
Date: Tue, 27 Sep 2005 09:45:23 -0500
Subject: [Microscopy] small system for ITO coatings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all- I'm in need of some ITO (indium-tin-oxide) coatings on glass
substrates and have obtained a few quotes of $800 or so. I can get an ITO
sputtering source (for about $500) that will fit in my lab DC sputtering
unit. The questions I have are: will a small sputtering unit be adaptable
to make good conductive as well as transparent ITO films?? Do I need to
introduce O2 as well as Ar to get the stoichiometry correct for the oxide?
What about the DC potential...is it OK at about 1KV? What about vacuum?

If anyone has already setup a small ITO coater maybe they can share their
experience... Thanks! Brian
____________________________________________________
Brian McIntyre
University of Rochester
Institute of Optics
RCEMLab
585-275-3058
585-244-4936 fax

"Be well, do good work, and keep in touch"


==============================Original Headers==============================
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From: yschwab-at-titus.u-strasbg.fr
Date: Tue, 27 Sep 2005 10:12:45 -0500
Subject: [Microscopy] EM facility user fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'm interested by the results of this query, would you mind sharing them
ON-list ?
Many thanks
Yannick

_________________________________________________________

Yannick Schwab
Service de Microscopie Electronique
IGBMC
1, rue Laurent Fries
67404 Illkirch Cedex
France
Tel +33(3) 88 65 56 06
Fax +33(3) 88 65 32 01
yschwab-at-igbmc.u-strasbg.fr
www-igbmc.u-strasbg.fr/MIF/mif.html
_________________________________________________________




mckee-at-HELIX.MGH.HARVARD.EDU wrote:

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==============================Original Headers==============================
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From: marc.pypaert-at-yale.edu
Date: Tue, 27 Sep 2005 10:50:10 -0500
Subject: [Microscopy] Re: EM facility user fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mary,

You can find the fees charged at Yale School of Medicine on our website:
http://cellserv.med.yale.edu/imaging/ccmi/elect_fees.html
Good luck

Marc


On Sep 27, 2005, at 9:34 AM, mckee-at-HELIX.MGH.HARVARD.EDU wrote:

}
}
}
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}
} Good morning again,
}
} Thanks to all of you who responded to my post about B/W printers - I'm
} digesting the info.
}
} Here's another query. I know that this has been discussed before,
} but I
} couldn't find the thread in the archives. We're re-evaluating what we
} charge our users for EM services (embedding, sectioning, scope
} time, etc)
} and would like to have an idea about what other academic institutions
} charge. If anyone is willing to discuss this, would you please e-
} mail me
} off-list or call me? Thanks.
}
} Mary
}
} Mary McKee
} Program in Membrane Biology
} Massachusetts General Hospital
} Boston, MA 02114
}
} (617)726-3696
} --
}
}
}
} ==============================Original
} Headers==============================
} 8, 16 -- From MCKEE-at-HELIX.MGH.HARVARD.EDU Tue Sep 27 08:32:49 2005
} 8, 16 -- Received: from PHSXCON5.partners.org
} (phsxcon5.mgh.harvard.edu [132.183.130.38])
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} 8, 16 -- User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.0.6
} 8, 16 -- Date: Tue, 27 Sep 2005 09:04:05 -0700
} 8, 16 -- Subject: EM facility user fees
} 8, 16 -- From: Mary McKee {mckee-at-HELIX.MGH.HARVARD.EDU}
} 8, 16 -- To: {microscopy-at-microscopy.com}
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} FILETIME=[F0D81CF0:01C5C367]
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} Headers==============================
}
}


==============================Original Headers==============================
7, 19 -- From marc.pypaert-at-yale.edu Tue Sep 27 10:50:09 2005
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7, 19 -- Date: Tue, 27 Sep 2005 11:50:05 -0400
7, 19 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
7, 19 -- Subject: Re: [Microscopy] EM facility user fees
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From: dave-at-boeckeler.com
Date: Tue, 27 Sep 2005 12:21:33 -0500
Subject: [Microscopy] Invitation to attend a one day Cryo SEM workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

You are cordially invited to attend a one day Cryo SEM workshop at the Carl
Zeiss facility in Thornwood, NY
The workshop is free of charge; lunch is provided courtesy of Carl Zeiss
SMT, Inc, but please BYOS (bring your own samples)
You have a choice of attending on Wednesday October 26 or Thursday October
27, 2005 but please register early since each workshop group is limited to 8
participants.
Workshop topics include: applications in Cryo Scanning Electron Microscopy;
Specimen preparation for Cryo SEM; High Pressure Freezing; Freeze Fracture,
etching & coating; Vacuum Cryo Transfer with lectures in the morning
followed by practical demonstrations after lunch on the Zeiss Ultra 55 FESEM
and Bal-Tec VCT 100 Cryo Transfer System.
The workshop agenda with application form can be located at:
www.baltec-rmc.com
or contact: Beth Bressan at Carl Zeiss, SMT, Inc: Bressan-at-smt.zeiss.com
or: Cheryl Johnson at Bal-Tec RMC Products, Boeckeler Instruments, Inc
Cheryl-at-boeckeler.com
for complete details


Dave Roberts
Boeckeler Instruments Inc
Tucson, Arizona
Tel: 520-745-0001
dave-at-boeckeler.com



==============================Original Headers==============================
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From: mark.grimson-at-ttu.edu
Date: Tue, 27 Sep 2005 18:17:47 -0500
Subject: [Microscopy] viaWWW: Microscope schematic posters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mark.grimson-at-ttu.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, September 27, 2005 at 11:08:30
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Email: mark.grimson-at-ttu.edu
Name: Mark Grimson

Organization: Texas Tech University

Title-Subject: [Filtered] MListserver: Microscope schematic posters

Question: Hello, I was wondering if anybody knows of a source of large, high quality schematic posters of TEMs, SEMs, confocal and fluorescent microscopes showing the relevant parts (lenes, etc) and ray path diagrams. These will be hung in the respective rooms and used primarily as teaching and reference aids as well as for aesthetics. Thanks.

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From: christopher.hayden-at-novartis.com
Date: Tue, 27 Sep 2005 18:18:27 -0500
Subject: [Microscopy] viaWWW: Preservation of Eyes

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (christopher.hayden-at-novartis.com) from http://www.microscopy.com/MLFormMail.html on Tuesday, September 27, 2005 at 12:12:56
---------------------------------------------------------------------------

Email: christopher.hayden-at-novartis.com
Name: Christopher Hayden

Organization: Novartis Pharmaceuticals

Title-Subject: [Filtered] Preservation of Eyes

Question: Good Afternoon, all:

Sorry to have to use the web form; for some reason our servers are rejecting my message when I tried to send it normally!

We have an upcomming project involving eyes from multiple species. Traditionally, we've collected samples in Mod. Karnovsky's fixative with no problems, but our opthamologist seems to remember a protocol involving sucrose that worked better. He can't find it in his literature, and we dug though our books and couldn't find a thing!

Does anyone have a special recipe that they find works well on eyes?

Thank you all!
-Chris

-----------------
Christopher Hayden
PCS/EM Lab
Novartis Pharmaceuticals
USEH 406/114
christopher.hayden-at-novartis.com


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From: cgarber-at-2spi.com
Date: Wed, 28 Sep 2005 00:10:48 -0500
Subject: [Microscopy] ITO coatings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Brian McIntyre wrote:
================================================
Hi all- I'm in need of some ITO (indium-tin-oxide) coatings on glass
substrates and have obtained a few quotes of $800 or so. I can get an ITO
sputtering source (for about $500) that will fit in my lab DC sputtering
unit. The questions I have are: will a small sputtering unit be adaptable
to make good conductive as well as transparent ITO films?? Do I need to
introduce O2 as well as Ar to get the stoichiometry correct for the oxide?
What about the DC potential...is it OK at about 1KV? What about vacuum?

If anyone has already setup a small ITO coater maybe they can share their
experience... Thanks! Brian
=================================================
Putting down the kinds of coatings of a quality most people want is a bit
more complicated than might initially appear.

If you are looking for ITO coated microscope slide or cover slip type glass,
these are standard products and are described on URL
http://www.2spi.com/catalog/standards/ITO-coated-slides-resistivities.html

If you need something custom, contact me off line and I could quote you
pricing for what you would be needing. You have not mentioned it, but the
resistivity, which depends on ITO coating thickness, and which will
ultimately determine the % transmittance, will have some influence on the
pricing.

Disclaimer: SPI Supplies offers ITO-coated glass slides and cover slips as
standard products.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




==============================Original Headers==============================
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8, 13 -- Subject: ITO coatings
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8, 13 -- From: "Garber, Charles A." {cgarber-at-2spi.com}
8, 13 -- X-Mailer: E-Mail Connection v3.1a
==============================End of - Headers==============================




From: vfink-at-shaw.ca
Date: Wed, 28 Sep 2005 08:44:22 -0500
Subject: [Microscopy] viaWWW: Looking for manuals & documents for Amray 1000A SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (vfink-at-shaw.ca) from http://www.microscopy.com/MLFormMail.html on Wednesday, September 28, 2005 at 00:37:47
---------------------------------------------------------------------------

Email: vfink-at-shaw.ca
Name: Victoria Fink

Organization: N/A

Title-Subject: [Filtered] MListserver: Looking for manuals & documents for Amray 1000A SEM

Question: Hi, All

I am looking for the manuals, or copies of any documents ( electrical schemes, etc.) for Amray 1000A SEM? My friend bought used one. Also, he is trying to find, and interested to purchase the electron gun for this instrument. Thank you in advance for your kind advise, and any help, information regarding to this matter.

Regards,



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From: ron.doole-at-materials.ox.ac.uk
Date: Wed, 28 Sep 2005 09:34:14 -0500
Subject: [Microscopy] EM Technical support vacancy at Oxford UK

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

We are currently seeking applicants for our Senior EM Support Engineer post
at Oxford. I would appreciate it if you could bring the attached
advertisement to the attention of anyone who you think may be interested in
the post.
Thanks,
Ron


Senior Electron Microscope Support Engineer
Grade: RAIA / Salary: £19,460 to £29,128 (pay award pending) / Job ref:
DJ05/020

The Department of Materials has an opportunity for an enthusiastic and
committed person to join the Electron Microscopy Technical Support Group
(TSG) to provide high-level technical support for the wide range of electron
optical instruments in the Department.

You will work as a member of EM Technical Support team with responsibility
for EM instrumentation throughout the Department but with particular
emphasis at the Begbroke site. You will liaise closely with the EM Research
Support Team for a wide range of duties involving fault diagnosis,
maintenance and repair of the Department's extensive range of electron
microscopes and ancillary equipment. As a senior member of the team, you
will take an active role in supporting users. On occasions you will deputise
for the Electron Microscope Senior Instrumentation Engineer.

A degree or equivalent in an appropriate field of engineering or technology
is essential, with at least 10 years experience of providing high technology
technical support and evidence of strong problem solving skills in this
environment. Applicants should have excellent interpersonal and
communication skills with some supervisory experience and/or evidence of the
aptitude and ability to develop good supervisory capabilities. They should
be able to work accurately and dependably with the minimum of supervision
and direction, and should have an interest in extending the capability of
instrumentation and in developing new ideas.

Applicants without the full range of experience or abilities will be
considered for appointment at a more junior level, with the intention of
providing in-house training, if a suitable candidate at the advertised level
is not found.

Any potential candidate who would like informal discussions about the post
is encouraged to contact Mr R Doole, tel 01865 273701 or
ron.doole-at-materials.ox.ac.uk.

Further particulars, including instructions on applying for this post, are
available from the web-site: http://www.materials.ox.ac.uk or from Mrs K
Fewings, Department of Materials, University of Oxford, Parks Road, Oxford
OX1 3PH (email: posts-at-materials.ox.ac.uk), or telephone 01865 273680 (post
reference DJ05/020). The closing date for applications is 28 October 2005
with interviews currently planned for 25 November 2005.

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk
http://www-em.materials.ox.ac.uk/
*********************************



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From: iztok.dogsa-at-ijs.si
Date: Wed, 28 Sep 2005 09:34:43 -0500
Subject: [Microscopy] viaWWW: FL digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (iztok.dogsa-at-ijs.si) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, September 28, 2005 at 09:22:04
---------------------------------------------------------------------------

Email: iztok.dogsa-at-ijs.si
Name: iztok

Organization: "Jozef Stefan" institute

Title-Subject: [Filtered] FL digital camera

Question: Hi all,

We are looking for low-budget color digital camera for fluorescence light microscopy. Our Micrscope is olympus BX-51. We intend to observe bacteria and biofilms.

Can you suggest a digital camera?

Thank you very much,
Iztok


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From: williams-at-pw.usda.gov
Date: Wed, 28 Sep 2005 10:51:52 -0500
Subject: [Microscopy] Embedding clay

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Can anyone suggest a good resin for embedding clay? We have tried Epon,
Spurrs, and LR White.

Thank you,

Tina Williams

USDA, CA, U.S.A.



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From: amr2w-at-cms.mail.virginia.edu
Date: Wed, 28 Sep 2005 15:04:45 -0500
Subject: [Microscopy] Dimpler Opinions/Suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tina,

Could you elaborate a bit more?
Why did the other resin's fail?
What solvents did you use to dehydrate the samples?
Are you just putting dried clay in the resin?
Is the problem at the section end, or the embedding end?
...

It would help to identify what direction to go in.

Regards,
Geoff Williams
Leduc Bioimaging Facility Manager
Brown University
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



Hey everyone,
Our group is interested in purchasing a new dimpler. Does anybody have
any opinions of which dimpler to purchase? We've been looking at ones by
South Bay, Gatan, and Fischione. They are all around the same price range,
so we're not concerned too much with cost, we just want to get the best
dimpler. Any feedback is appreciated! Thank you!
-Andrew Roelant

==============================Original Headers==============================
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From: cgarber-at-2spi.com
Date: Wed, 28 Sep 2005 15:08:02 -0500
Subject: [Microscopy] Embedding of clay

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Tina Williams wrote:
===============================================
Can anyone suggest a good resin for embedding clay? We have tried Epon,
Spurrs, and LR White.
================================================
"Epon" should work, at least our own SPI-Pon 812 resin works in our own
laboratory and when there have been problems with platelet "pull out" during
sectioning, adhesion with the embedding resin can be enhanced through the
use of 3GTMO, see URL
http://www.2spi.com/catalog/chem/trimethoxysilane.shtml

If you are embedding entire clumps (I think there is a more technical word
than "clumps"), a vacuum embedding would be more appropriate in order to get
complete infiltration of the resin.

You should not have to rush out and purchase SPI-Pon 812, at least some of
the other "Epon substitutes" should work just as well, but not necessarily
all of them since they are not all the same. One trick for making it "work"
is to cure to a harder rather than a softer block.

If none of these suggestions work, let me know, as there are some other
tricks on can consider using, depending on the circumstances of your clay
samples.

Disclaimer: SPI Supplies is a supplier of both SPI-Pon 812 and also the
adhesion promoting compound, 3GTMO.

Chuck

PS: Remember that we are 100% paperless and the only way we can keep track
of this kind of correspondence is if you reply using the reply feature of
your e-mail software. That way the entire string of correspondence will be
in one place.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================






==============================Original Headers==============================
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10, 13 -- Date: Wed, 28 Sep 2005 16:08:04 -0500
10, 13 -- From: "Garber, Charles A." {cgarber-at-2spi.com}
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==============================End of - Headers==============================




From: danon-at-cnea.gov.ar
Date: Wed, 28 Sep 2005 15:21:06 -0500
Subject: [Microscopy] More on DTSA spectrum analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everybody,

first of all, I would like to thank all who responded my question on DTSA
spectrum analysis last week. The problem I had was that our version of the
EDAX-DX4 software (2.11) was not the right one to get the .spc files
converted to .msa files; in fact it was a very old version and did not
contain the utility SpecUtility.exe. Once the version was changed, it worked
and I got my files converted.
But...the problem is not completely solved, as DTSA seems aparently not to
import the converted .msa files, with none of the two plugins specified for
that task, so any clue will be appreciated.

Best regards,

Ariel Danon
Materials Department
National Commission of Atomic Energy
Buenos Aires, Argentina


==============================Original Headers==============================
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5, 24 -- To: {Microscopy-at-MSA.Microscopy.com}
5, 24 -- Subject: More on DTSA spectrum analysis
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From: macone-at-med.unc.edu
Date: Wed, 28 Sep 2005 15:35:00 -0500
Subject: [Microscopy] viaWWW: American Optical 860 Sliding Microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (macone-at-med.unc.edu) from http://www.microscopy.com/MLFormMail.html on Wednesday, September 28, 2005 at 10:27:23
---------------------------------------------------------------------------

Email: macone-at-med.unc.edu
Name: Kirk McNaughton

Organization: UNC Cell and Molecular Physiology

Title-Subject: [Filtered] Sliding Microtome

Question:
I know this is way out on a limb, but does anyone have a manual for an American Optical 860 Sliding Microtome?

We have the Microtome and could really use the maunal (or copy of) to guide us in the right direction.

Any help is appreciated.

Thank you,
Kirk McNaughton
University of North Carolina
Dept. Cell and Molecular Physiology
Room 5105, Neuroscience Research Building
105 Mason Farm Road
Chapel Hill, NC 27599-7545
(919) 966-1202
(919) 966-6927 fax
macone-at-med.unc.edu


---------------------------------------------------------------------------

==============================Original Headers==============================
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From: henriks-at-southbaytech.com
Date: Wed, 28 Sep 2005 15:40:54 -0500
Subject: [Microscopy] Re: Dimpler Opinions/Suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Andrew:

I thought it might be helpful to clarify that South Bay Technology
actually manufactures 2 Dimplers®. The Model 515 Precision Dimpling
Instrument and the D500i Dimpler®. The D500i was previously produced by
VCR Group. South Bay Technology acquired VCR Group back in 1998. I
won't offer you my opinion of which Dimpler® to purchase as I doubt it
would be that useful coming from one of the manufacturers! Of course, I
would be available off-line to discuss any of your requirements.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David

--
David Henriks

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: +1-949-492-2600
Toll-free in the USA: +1-800-728-2233
FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

Celebrating 40 years of providing Materials Processing Solutions for Metallogaphy, Crystallography and Electron Microscopy.

Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.



amr2w-at-cms.mail.virginia.edu wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America




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From: tonygr-at-MIT.EDU
Date: Wed, 28 Sep 2005 16:12:38 -0500
Subject: [Microscopy] Re: More on DTSA spectrum analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm speaking from memory here - I haven't checked my old notes, but I think
I may have seen a problem like this before.

The MSA file specification is very precise (it is available from the MSA
web site), and specifically requires that the data values be given as real,
not integer, numbers, and is also very specific about how the delimiters
(commas, if my memory serves) are placed (for example, the last datapoint
on a line has to have the format "xxx. {ret} " where the {ret} means the end
of line, and no extra spaces are allowed. I don't think that the EDAX
specimen conversion utility follows this convention properly, and instead
writes numbers without the decimal point.

(I have EDAX, Rontec and Oxford systems - I'm certain that the Oxford files
read into DTSA fine, and I don't recall ever trying with Rontec, so I'm
pretty certain it is the EDAX ones I had the problem with.)

DTSA follows the convention precisely, and then gets confused reading in
the converted files. You can read the file into a spreadsheet and adjust
the format there (which is what I have done when I have needed to take
files into DTSA), or it would be simple to write a Basic utility to
reformat the file (but I haven't done that).

Better, EDAX could correct their utility!

Tony.


} Hello everybody,
}
} first of all, I would like to thank all who responded my question on DTSA
} spectrum analysis last week. The problem I had was that our version of the
} EDAX-DX4 software (2.11) was not the right one to get the .spc files
} converted to .msa files; in fact it was a very old version and did not
} contain the utility SpecUtility.exe. Once the version was changed, it worked
} and I got my files converted.
} But...the problem is not completely solved, as DTSA seems aparently not to
} import the converted .msa files, with none of the two plugins specified for
} that task, so any clue will be appreciated.
}
} Best regards,
}
} Ariel Danon
} Materials Department
} National Commission of Atomic Energy
} Buenos Aires, Argentina
}
}
} ==============================Original Headers==============================
} 5, 24 -- From danon-at-cnea.gov.ar Wed Sep 28 15:21:06 2005
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} 17:29:20 -0300
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} 5, 24 -- From: "Ariel Danon" {danon-at-cnea.gov.ar}
} 5, 24 -- To: {Microscopy-at-MSA.Microscopy.com}
} 5, 24 -- Subject: More on DTSA spectrum analysis
} 5, 24 -- Date: Wed, 28 Sep 2005 05:22:43 -0300
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} ==============================End of - Headers==============================

***********************************************
Anthony J. Garratt-Reed, M.A., D.Phil.
MIT Room 13-1027
77 Massachusetts Avenue
Cambridge, MA 02139-4307
USA

Tel: 617-253-4622
Fax: 617-258-6478
************************************************


==============================Original Headers==============================
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From: williams-at-pw.usda.gov
Date: Wed, 28 Sep 2005 16:35:25 -0500
Subject: [Microscopy] RE: Embedding clay

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello again,

This is regarding the earlier questions about embedding clay.

We used water/ ethanol, propylene oxide, and water/ acetonitrile for
dehydration. We found problems with poor infiltration (in Epon, Spurr's,
or LR White). Thus, sectioning problems. We are willing to try other
dehydration protocols. Today it was suggested to try cryoultramicrotomy,
however we do not have a set up for this.

Thanks to all those who have responded. Please keep the suggestions coming.

Tina

USDA, CA, U. S. A.



==============================Original Headers==============================
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8, 18 -- Subject: [Microscopy] RE: Embedding clay
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From: davilla-at-4pi.com
Date: Wed, 28 Sep 2005 16:47:59 -0500
Subject: [Microscopy] Re: More on DTSA spectrum analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} The MSA file specification is very precise (it is available from the MSA
} web site), and specifically requires that the data values be given as real,
} not integer, numbers, and is also very specific about how the delimiters
} (commas, if my memory serves) are placed (for example, the last datapoint
} on a line has to have the format "xxx. {ret} " where the {ret} means the end
} of line, and no extra spaces are allowed. I don't think that the EDAX
} specimen conversion utility follows this convention properly, and instead
} writes numbers without the decimal point.
}

DTSA also expects Y msa format (multiple columns of counts) so if
your msa exporter does XY format (two columns of energy and count at
that energy), DTSA will not import the file properly. Both formats
are correct in the eyes of the msa format, the msa importer must be
smart enough to handle both.

Also watch out for the end of line terminator. There are three types,
dos, mac and unix. The msa standard does not define which flavor is
expected so a smart reader needs to handle all flavors. So if you are
exporting on a dos/win computer and importing on a MacOS computer you
will need to convert the end of line terminator.

If you want an easy way to do this, download our Revolution program.
It will run in demo mode but this mode will allow msa import and msa
export. Both X and XY are handled for both import and export.
Revolution runs on both WinOS and MacOS platforms. We can handle just
about every msa file I've come across and if you have one that has a
problem, sent it to us and we will fix the issue.

Scott
--
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com


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6, 17 -- Subject: [Microscopy] Re: More on DTSA spectrum analysis
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==============================End of - Headers==============================




From: shields-at-ftw.com
Date: Wed, 28 Sep 2005 20:35:05 -0500
Subject: [Microscopy] AskAMicroscopist: Zeiss Ultraphot II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (shields-at-ftw.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, September 28, 2005 at 19:11:11
---------------------------------------------------------------------------

Email: shields-at-ftw.com
Name: Neal Shields

Organization: none

Education: Undergraduate College

Location: Ft. Worth Texas USA

Question: I just purchased an Zeiss Ultraphot II for use in my photography hobby.

It has a plug with two round prongs on the microscope body for the electronics in the photo head.

I have a powersupply drawer but it doesn't look complete, requires 220 and is huge. (my wife already doesn't like the size of the microscope)

Can anyone tell me what the input voltage and amperage needs to be and possiably suggest a power supply?

Thanks

Neal

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: PetrS-at-ISIBrno.Cz
Date: Thu, 29 Sep 2005 01:52:06 -0500
Subject: [Microscopy] Meetings for Microscopists in 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Microscopists,

A major update of the list of meetings for microscopists in the year
2006 has been finished at the Petr's Microscopy Resources at the

http://www.petr.isibrno.cz/microscopy/meetings.php#2006

Your submission of other meetings will be very appreciated (submission
form is accessible from the page mentioned, the direct link for the
submission is http://www.petr.isibrno.cz/microscopy/PMRform.php).

Petr Schauer
+--------------------------------------------------------------------+
| Dr. Petr Schauer tel.: +420 541 514 313 |
| Head of the Group of the Scintillation fax : +420 541 514 404 |
| and Cathodoluminescent Systems +420 541 514 402 |
| INSTITUTE OF SCIENTIFIC INSTRUMENTS |
| ACADEMY OF SCIENCES OF THE CZECH REPUBLIC e-mail: petr-at-isibrno.cz |
| Kralovopolska 147, CZ-612 64 Brno petr-at-dp.fce.vutbr.cz |
| Czech Republic www: http://www.petr.isibrno.cz/ |
+--------------------------------------------------------------------+


==============================Original Headers==============================
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From: farmstead-at-geo-centers.com
Date: Thu, 29 Sep 2005 07:59:10 -0500
Subject: [Microscopy] Electron Microscopy Opportunities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Geo-Centers, Inc. is seeking to fill Electron Microscopy opportunities in the Maryland area, which will support our efforts with NBACC/NBFAC (The National Bioforensic Analysis Center). The National Bioforensic Analysis Center (NBFAC) was designated by Homeland Security Presidential Directive 10 (HSPD10) to be the lead federal agency to conduct and coordinate forensic analysis of evidentiary material from biocrime or bioterrorist events. The NBFAC operates the first biocontainment laboratories solely dedicated to coordinating and conducting bioforensic analyses to generate data for attribution analysis of biocrimes, bioterrorism, or state-sponsored biological events. NBFAC will provide law enforcement agencies, the intelligence community, the State Department, and the Department of Defense (DoD) with centrally coordinated, validated sample handling and processing, and bioforensic analysis of evidence and samples to generate data for attribution analysis in support of national and homeland security. Positions:

Ph.D Lab Manager: The desired candidate will be a Ph.D. level electron microscopist with industrial or research experience performing transmission and scanning electron microscopy of bacteria and viruses. Expert in all areas of scanning and transmission microscopy of bacteria and viruses, to include elemental analysis (EDX). Experience in preparation and examination of samples. Capable of developing and writing laboratory standard operating procedures and managing an activity in compliance with ISO 17025 standards.

Support Technician: The desired candidate will be a BS-MS level laboratory technician with industrial and/or research experience with transmission (TEM) and scanning electron microscopy (SEM). Experience in transmission and scanning electron microscopy of bacteria and viruses including preparation and examination of EM samples is required; experience with elemental analysis (EDX) highly desired. Supporting the development and writing laboratory standard operating procedures and managing an activity in compliance with ISO 17025 standards. Capable of the conduct of laboratory operations within a BSL-2/3 environment.

Must be able to obtain select agent status and a SECRET clearance. US Citizenship required.
Visit our website at www.geo-centers.com to view position descriptions. Forward resumes in word format with salary requirement to staffing-at-geo-centers.com attn: ELAB-FA-MM.


==============================Original Headers==============================
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From: pmmcm-at-bellsouth.net
Date: Thu, 29 Sep 2005 07:59:28 -0500
Subject: [Microscopy] AskAMicroscopist: stereomicroscope for high school

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pmmcm-at-bellsouth.net) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, September 28, 2005 at 20:16:05
---------------------------------------------------------------------------

Email: pmmcm-at-bellsouth.net
Name: Pamela McMurray

Organization: (homeschool)

Education: 9-12th Grade High School

Location: Lawrenceville, Georgia, USA

Question: We are planning to purchase a stereomicroscope for high school biology, earth science, etc., plus looking at fossils, and had a question about recommended magnification. The scope we are looking at comes in 10/20, 10/30, or 20/40 magnification. There is also a .5 reducer and 2x supplementary lens avail. as well as higher power eyepieces. What magnification might be best for our purposes?

Thanks so much.

---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-ultra5.microscopy.com Thu Sep 29 07:59:28 2005
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8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: pmmcm-at-bellsouth.net (by way of Ask-A-Microscopist)
8, 12 -- Subject: AskAMicroscopist: stereomicroscope for high school
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: macone-at-med.unc.edu
Date: Thu, 29 Sep 2005 08:00:55 -0500
Subject: [Microscopy] viaWWW: Thanks-AO 860 sliding/sledge microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (macone-at-med.unc.edu) from http://www.microscopy.com/MLFormMail.html on Thursday, September 29, 2005 at 07:23:42
---------------------------------------------------------------------------

Email: macone-at-med.unc.edu
Name: Kirk McNaughton

Organization: Cell and Molecular Physiology

Title-Subject: [Filtered] AO 860 (sliding/sledge) Microtome

Question:
Many thanks to all for their very prompt response to my request for an AO 860 sliding/sledge microtome manual.
I recieved some good web site locations as well as an offer to send a copy of this manual my way.

Sincerely,
Kirk

Kirk McNaughton
Research Analyst
University of North Carolina
Dept. Cell and Molecular Physiology
Room 5105, Neuroscience Research Building
105 Mason Farm Road
Chapel Hill, NC 27599-7545
(919) 966-1202
(919) 966-6927 fax
macone-at-med.unc.edu





---------------------------------------------------------------------------

==============================Original Headers==============================
11, 12 -- From zaluzec-at-microscopy.com Thu Sep 29 08:00:55 2005
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11, 12 -- From: macone-at-med.unc.edu (by way of MicroscopyListserver)
11, 12 -- Subject: viaWWW: Thanks-AO 860 sliding/sledge microtome
11, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: NWWhite-at-bwxt.com
Date: Thu, 29 Sep 2005 12:16:54 -0500
Subject: [Microscopy] Nikon Trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

Has anyone experienced (and fixed) the following Nikon Coolpix 995
problem?

Camera reports: "This card cannot be used" - on power-up.

-Battery is OK.
-Have tried camera system reset.
-CF card works in other similar Nikons.
-Working card from another Nikon presents same problem in troubled
camera.
-No bent pins.
-Pin corrosion unlikely - Camera used little and well kept. Also, card
has been in/out a number of times.
-Camera will skip the format selection in the menu - Not selectable.
-Camera appears to function normally otherwise - what functions there
are with no card, anyway.

Thanks,

Woody White
BWXT Services



==============================Original Headers==============================
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8, 26 -- From: "White, Woody N." {NWWhite-at-bwxt.com}
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From: neuberger1234-at-comcast.net
Date: Thu, 29 Sep 2005 12:45:03 -0500
Subject: [Microscopy] Nikon Trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Woody,

I have had the same problem with another Nikon camera and found that it was
the card being formatted incorrectly. What I did was put it into a card
reader on a PC and then formatted it to FAT and it worked OK. The card
company told me as did the local Nikon repair facility that I should then
format the card in the camera a couple of times. DO NOT use FAT32 as it
will give you that error. Whether this is the problem, I'd like to know.

Damian Neuberger, Ph.D.
Consultant
Microscopy/Digital Imaging/Image Analysis
2416 Covert Rd
Glenview IL 60025
Tel: (847) 998-8574
email: neuberger1234-at-comcast.net {mailto:neuberger1234-at-comcast.net}



-----Original Message-----
X-from: NWWhite-at-bwxt.com [mailto:NWWhite-at-bwxt.com]
Sent: Thursday, September 29, 2005 12:17 PM
To: neuberger1234-at-comcast.net

Hello All,

Has anyone experienced (and fixed) the following Nikon Coolpix 995
problem?

Camera reports: "This card cannot be used" - on power-up.

-Battery is OK.
-Have tried camera system reset.
-CF card works in other similar Nikons.
-Working card from another Nikon presents same problem in troubled
camera.
-No bent pins.
-Pin corrosion unlikely - Camera used little and well kept. Also, card
has been in/out a number of times.
-Camera will skip the format selection in the menu - Not selectable.
-Camera appears to function normally otherwise - what functions there
are with no card, anyway.

Thanks,

Woody White
BWXT Services



==============================Original Headers==============================
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==============================Original Headers==============================
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22, 23 -- Subject: RE: [Microscopy] Nikon Trouble
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From: bozzola-at-siu.edu
Date: Thu, 29 Sep 2005 13:03:14 -0500
Subject: [Microscopy] Ultramicrotomes compared

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been asked by our Purchasing folks to justify purchase of an
ultramicrotome.

I would like feedback from users of the Leica Ultracut EM UC6 versus
the RMC PowerTome.

All responses will be kept confidential if you respond directly to me
rather then the list.

Thank you.


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################

==============================Original Headers==============================
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From: tivol-at-caltech.edu
Date: Thu, 29 Sep 2005 13:11:12 -0500
Subject: [Microscopy] Re: AskAMicroscopist: stereomicroscope for high school

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Sep 29, 2005, at 5:59 AM, pmmcm-at-bellsouth.net wrote:

}
} Question: We are planning to purchase a stereomicroscope for high
} school biology, earth science, etc., plus looking at fossils, and had
} a question about recommended magnification. The scope we are looking
} at comes in 10/20, 10/30, or 20/40 magnification. There is also a .5
} reducer and 2x supplementary lens avail. as well as higher power
} eyepieces. What magnification might be best for our purposes?
}
} Thanks so much.
}
Dear Pamela,
If you go to the MSA web site, http://www.msa.microscopy.org/, and
scroll down to Reference and Educational Activities, you will find
Project MICRO, which has information and recommendations for the kinds
of investigations you wish to do.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: Ralph.Common-at-hc.msu.edu
Date: Thu, 29 Sep 2005 15:02:04 -0500
Subject: [Microscopy] substrate for cell culture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A client needs to have cell cultures of endothelial cells embedded for TEM. I would be grateful to hear (off line) specific recommendations for a substrate for growing the cells. The substrate will have to withstand ethanol and propylene oxide, and have good sectioning properties when used with an Epon type resin. I would also appreciate recommendations for fixation.

Ralph Common
Division of Human Pathology
Michigan State University



==============================Original Headers==============================
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From: Elliott-at-arizona.edu
Date: Thu, 29 Sep 2005 15:12:38 -0500
Subject: [Microscopy] Re: substrate for cell culture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have many times grown endothelial cells on Aclar Film. EtOH
sterilize the film. Put it in the bottom of the TC dish. Add your
cells. Some of them will grow on the bottom of the film. No problem.
Fix and embed the film with the cells. The film, in my hands, is
impervious to all treatments. When the block is hard, just peel off
the film and your cells are in the plastic. You can section in either
orientation, as your want.
David


On Sep 29, 2005, at 1:07 PM, Ralph.Common-at-hc.msu.edu wrote:

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} A client needs to have cell cultures of endothelial cells embedded for
} TEM. I would be grateful to hear (off line) specific recommendations
} for a substrate for growing the cells. The substrate will have to
} withstand ethanol and propylene oxide, and have good sectioning
} properties when used with an Epon type resin. I would also appreciate
} recommendations for fixation.
}
} Ralph Common
} Division of Human Pathology
} Michigan State University
}
}
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From: TindallR-at-missouri.edu
Date: Thu, 29 Sep 2005 16:47:01 -0500
Subject: [Microscopy] substrate for cell culture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One commonly used substrate is the Thermonox line of cover slips,
available from many suppliers. They come in various sizes and the cells
can be grown on them, then the coverslips with cells fixed, dehydrated
and embedded as usual. The section quite well, except for an
unfortunate tendency for sections not to detach completely in the boat
and to pull the next section back over the edge of the knife. This can
be remedied by making a pointy block face or by using a razor blade to
make a line just beneath the top of the block face so the section
detaches just before the end of the cutting stroke.

The cover slips can be cut into pieces small enough to embed in ordinary
capsules and are normally used to do cross-sections through cell layers,
as opposed to "top down" sections. With care and creativity you should
also be able to get other orientations, but it would be much trickier.

I'm not 100% sure of their compatibility with PO, but I don't believe
it's a problem. They stand up fine to EPON and other resins we use.

An alternative is to grow the cells on any old cover slips, run them
through the fixation, dehydration, and infiltration steps, then place
them cell side down on top of resin filled (with a resin meniscus
slightly up over the top) embedding capsules. Polymerize them as usual,
taking care that they are level so the cover slips don't slide off.
After everything is mostly hard, immerse the coverslip/capsule assembly
in liquid nitrogen, then snap off the coverslip from the capsule. If
all goes well, and it usually does, the cells with transfer to the resin
in the capsule and form the top layer of the block. Identify your area
under a light microscope, trim away the rest (careful!), then start
taking sections. Remember that there is only one cell layer----if you
cut through it and discard it by mistake, it's gone.

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu









-----Original Message-----
X-from: Ralph.Common-at-hc.msu.edu [mailto:Ralph.Common-at-hc.msu.edu]
Sent: Thursday, September 29, 2005 3:05 PM
To: Tindall, Randy D.

A client needs to have cell cultures of endothelial cells embedded for
TEM. I would be grateful to hear (off line) specific recommendations
for a substrate for growing the cells. The substrate will have to
withstand ethanol and propylene oxide, and have good sectioning
properties when used with an Epon type resin. I would also appreciate
recommendations for fixation.

Ralph Common
Division of Human Pathology
Michigan State University



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From: beth.moseley-at-hitachi-hta.com
Date: Thu, 29 Sep 2005 17:26:43 -0500
Subject: [Microscopy] Hitachi High Technologies Nanotechnology Seminar Series

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Listers,

{} You are cordially invited to attend Hitachi High Technologies America,
Inc., third annual
three city free nanotechnology seminar series during the week of October
17- 21, 2005.

The first day long seminar will take place on Monday, October 17, 2005 at the Omni Hotel at
Southpark in Austin, Texas. The series will be repeated in Albany, New York on Wednesday,
October 19, 2005 and in Santa Cruz, California on Friday, October 21, 2005. Tours of the
seminar host facilities will also be available at two of the sites for attendees.

All of the seminars are free of charge and will feature presentations by renowned microscopy
experts and scientists. For more information about location, times, and scheduled speakers
please check under events on the Hitachi web site at www.hitachi-hta.com {http://www.hitachi-hta.com/}


Nanotechnology and the Semiconductor Industry
October 17, 2005 9:00 am – 5:00 pm Omni Hotel at Southpark – Austin, TX
Please register by Oct. 10 at: austinnano-at-hitachi-hta.com
{mailto:austinnano-at-hitachi-hta.com}

Nanotechnology and the Semiconductor Industry
October 19, 2005 9:00 am – 5:00 pm
Location: CESTM Albany NanoTech Albany, NY
Please register by Oct. 10 at: albanynano-at-hitachi-hta.com
{mailto:albanynano-at-hitachi-hta.com}

Nanotechnology: The Intersection of Materials and Life Sciences
October 21, 2005 9:00 am – 5:00 pm
University Center University of California – Santa Cruz Santa Cruz, CA
Please register by Oct. 10 at: santacruznano-at-hitachi-hta.com
{mailto:santacruznano-at-hitachi-hta.com}


Beth Moseley
Hitachi High Technologies America
Tel: 925.218.2821
beth.moseley-at-hitachi-hta.com {mailto:beth.moseley-at-hitachi-hta.com}


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From: support-at-isi.ir
Date: Thu, 29 Sep 2005 19:20:39 -0500
Subject: [Microscopy] AskAMicroscopist:Service for JEOL 733

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Saturation with MMA (methyl methacrylate) followed by polymerisation with Co-60
gamma radiation works extremely well and reliably, but first find your Co-60 source.

cheers

rtch




Date sent: Wed, 28 Sep 2005 16:36:10 -0500
To: r.sims-at-auckland.ac.nz
X-from: williams-at-pw.usda.gov
Send reply to: williams-at-pw.usda.gov

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (support-at-isi.ir) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, September 29, 2005 at 10:18:16
---------------------------------------------------------------------------

Email: support-at-isi.ir
Name: Ardavan

Organization: IDEA Inc.

Education: Graduate College

Location: Tehran , Iran

Question: I need an expert engineer to maintain and calibrate a JEOL 733 system in Iran.

With Best Regards
Ardavan

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From: fulton.2-at-osu.edu
Date: Thu, 29 Sep 2005 19:21:44 -0500
Subject: [Microscopy] viaWWW: preparing coated grids for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (fulton.2-at-osu.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Thursday, September 29, 2005 at 15:42:55
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Email: fulton.2-at-osu.edu
Name: Dave Fulton

Organization: OSU/OARDC

Title-Subject: [Filtered] MListserver:TEM

Question: We have been preparing coated grids for TEM for a long time, with relatively little trouble. We follow the protocol for preparation of formvar coated grids from the Bozzola and Russel text, Electron Microscopy. I recently tried to prepare grids with very little success. I was using fresh formvar solution from EMS, thinking that our old solution might be the problem. I tried several different brands of slides as well, all leading to no success. Have any of you out there experienced similar problems? Any ideas would be greatly appreciated. You may reply offline if you wish. Thank you.






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From: tylko-at-zuk.iz.uj.edu.pl
Date: Fri, 30 Sep 2005 01:08:36 -0500
Subject: [Microscopy] vacuum pumps and LaB6

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

My department would like to improve the vacuum system in our old scanning
electron microscope JEOL JSM 5410, which is equipped with Si(Li) EDS
detector. The main reason for this step is to prolong the working time of
the tungsten filament and/or replace this type of the source with LaB6. We
would like to diminish oil contamination coming from roughing and diffusion
pumps as well. We have got two ideas concerning this matter. One option is
to attach ion pump to the existing pumping system to improve vacuum to 10-7
mBarr and the second option is to remove diffusion pump and replace it with
turbomolecular pump. My question is the following: What are advantages and
disadvantages of such upgrade of the vacuum system? What kind of pump should
be installed to our microscope? Have you got any experience with these two
types of pumps? Is this modification worth doing?

Another question concerns the electron gun of JEOL JSM 5410. Should we
change the tungsten emitter into LaB6 if we would like to perform X-ray
microanalysis? I heard that this type of gun is not recommended for
quantitative X-ray microanalysis due to its poor stability. It is also not
so easy to do such modification I suppose... Could you recommend us
something in this matter as well?



Best regards,


Dr Grzegorz Tylko
Department of Cytology and Histology
Institute of Zoology
Jagiellonian University
ul. Ingardena 6
30-060 Krakow
fax: +48-12-634-49-51
phone: +48-12-633-63-77 ext. 2425
mobile phone: +48-602-535-041
e-mail: tylko-at-zuk.iz.uj.edu.pl


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From: dljones-at-bestweb.net
Date: Fri, 30 Sep 2005 08:32:16 -0500
Subject: [Microscopy] Re: vacuum pumps and LaB6

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Chris,

As an institute of ophthalmology I suppose we should be able to help - but
I'm now in the cell biology department where tissues are slightly frowned
apon.

Generally sucrose solutions are regularly used as a cryoprotectant. For
non-cryogenic light microscopy, sucrose immersion is generally a poor choice
for paraffin wax embedding (I always used Spurr's, LR White resins etc. as
my work always involved considerable image analysis). If you use
paraformaldehyde only (rather than with Gluta-aldehyde) some do add sucrose
to stop strange effects (like blebbing in fixed cells) but this again this
may affect the tissue for paraffin wax embedding.

Glutaladehyde is regularly combined with paraformaldehyde, as in Karnovsky's
fixative, to improve things. These fixatives can be used to fix tissues for
paraffin embedding, but the tissues will be quite brittle (which is why
sucrose is occasionally used, and why I used resin embedding). Standard
gluta-aldehyde recipes are used here to fix eyes that are going to be
embedded for TEM and ultra-sectioning.

Glutaraldehyde is a fixative regularly used for electron microscopic
studies, and I always used this as a secondary fixative after initially
inflating and fixing lungs in osmium tetroxide dissolved in Freon FC-80 - a
method that even fixed macrophages in-situ on airways. I used to prepare
samples more in a TEM manner as I required high quality sections for lung
distribution and micro-dosimetry studies (often with CR-39 autoradiographs
coupled with serial stained histology). Once fixed over a few hours, fixed
tissues were then stored refrigerated in cacodylate buffer (that often has a
little sucrose to help with the osmotic pressure). Osmium fixes lipids
rather than just proteins so really helps on section quality although its
highly toxic properties and 'creep' are a real pain (although once its black
it's osmium dioxide and stable - when its toxic it's invisible).

Unfortunately all my relevant research papers and fixation recipes are up in
the attic at home somewhere, so I am writing from memory. My recipes were no
doubt similar to that found on the web today.

Hope this is of some help.

regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk




----- Original Message -----
X-from: {christopher.hayden-at-novartis.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Wednesday, September 28, 2005 12:25 AM

Dr Grzegorz Tylko,

I am not intimately familiar with the specific SEM you are referring to, but I
will comment a bit on the questions you've asked. If you could answer some
questions, then a better opinion could be formed.

Can you perform light element analysis with your EDS detector?

What pumping oil is used in your diffusion pump?

Generically, the vacuum upgrade would be best done by removing the diffusion
pump and putting in a turbomolecular pump. There are vibration isolation issues
with this upgrade that you have to address carefully. If you were to put an ion
pump onto the diffusion pumped system, how would you connect it if this is being
done on the chamber? I wonder about the lifespan of your ion pump if it is used
to pump the diffusion pump vapors from your diffusion pump. The LaB6 systems I
have worked on have an ion pump connected to the filament side of the SEM. The
vacuum piping that runs to the filament has an isolation valve that seperates
the head of the gun from the chamber. An ion pump is connected at the electron
gun head and always pumps the head of the gun.

The instrument I currently use is set-up to run both W and LaB6 filaments. I
never use the LaB6 option.

For what's it worth....

dj

On Fri, 30 Sep 2005 tylko-at-zuk.iz.uj.edu.pl wrote:

}
}
}
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} Dear Colleagues,
}
} My department would like to improve the vacuum system in our old scanning
} electron microscope JEOL JSM 5410, which is equipped with Si(Li) EDS
} detector. The main reason for this step is to prolong the working time of
} the tungsten filament and/or replace this type of the source with LaB6. We
} would like to diminish oil contamination coming from roughing and diffusion
} pumps as well. We have got two ideas concerning this matter. One option is
} to attach ion pump to the existing pumping system to improve vacuum to 10-7
} mBarr and the second option is to remove diffusion pump and replace it with
} turbomolecular pump. My question is the following: What are advantages and
} disadvantages of such upgrade of the vacuum system? What kind of pump should
} be installed to our microscope? Have you got any experience with these two
} types of pumps? Is this modification worth doing?
}
} Another question concerns the electron gun of JEOL JSM 5410. Should we
} change the tungsten emitter into LaB6 if we would like to perform X-ray
} microanalysis? I heard that this type of gun is not recommended for
} quantitative X-ray microanalysis due to its poor stability. It is also not
} so easy to do such modification I suppose... Could you recommend us
} something in this matter as well?
}
}
}
} Best regards,
}
}
} Dr Grzegorz Tylko
} Department of Cytology and Histology
} Institute of Zoology
} Jagiellonian University
} ul. Ingardena 6
} 30-060 Krakow
} fax: +48-12-634-49-51
} phone: +48-12-633-63-77 ext. 2425
} mobile phone: +48-602-535-041
} e-mail: tylko-at-zuk.iz.uj.edu.pl
}
}
} ==============================Original Headers==============================
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From: YANGA-at-AGR.GC.CA
Date: Fri, 30 Sep 2005 09:03:42 -0500
Subject: [Microscopy] vacuum pumps and LaB6

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dr. Tylko,

I have experience with both pumps. An Ion pump is mounted to the gun chamber. It will not solve your oil vapour problem. You can install a liquid nitrogen baffle (if you still can find it in the market, one of the instruments I used before had it) above the diffusion pump to trap oil vapour. I think this is least expensive to solve oil vapour problem, but you need liquid nitrogen. The next choice is what you have thought of, i.e. change DP to Turbo pump with some modification of the circuit.

An Ion gun is different from a tungsten gun; and there is an Ion pump attached to the gun chamber. So, I don't think that you can simply switch to LaB6 emitter in a tungsten gun. LaB6 will fail quickly without an Ion pump and it is expensive. An Ion pump can only be started up when the vacuum is very good, say 10-6 Torr. The LaB6 crystal must be warmed up slowly. You may find better resolution but do you really need it? We do not think so, and our instruments are without such an expensive option now. If you want a brighter and more stable beam for X-ray analysis, that is another subject which I am unable to comment on.

Ann Fook Yang
EM Unit/ Unite EM
AAFC/AAC
960 Carling Ave,
Ottawa,Ontario
Canada K1A 0C6
yanga-at-agr.gc.ca
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
X-from: tylko-at-zuk.iz.uj.edu.pl [mailto:tylko-at-zuk.iz.uj.edu.pl]
Sent: Friday, September 30, 2005 2:12 AM
To: Yang, Ann-Fook

Dear Colleagues,

My department would like to improve the vacuum system in our old scanning
electron microscope JEOL JSM 5410, which is equipped with Si(Li) EDS
detector. The main reason for this step is to prolong the working time of
the tungsten filament and/or replace this type of the source with LaB6. We
would like to diminish oil contamination coming from roughing and diffusion
pumps as well. We have got two ideas concerning this matter. One option is
to attach ion pump to the existing pumping system to improve vacuum to 10-7
mBarr and the second option is to remove diffusion pump and replace it with
turbomolecular pump. My question is the following: What are advantages and
disadvantages of such upgrade of the vacuum system? What kind of pump should
be installed to our microscope? Have you got any experience with these two
types of pumps? Is this modification worth doing?

Another question concerns the electron gun of JEOL JSM 5410. Should we
change the tungsten emitter into LaB6 if we would like to perform X-ray
microanalysis? I heard that this type of gun is not recommended for
quantitative X-ray microanalysis due to its poor stability. It is also not
so easy to do such modification I suppose... Could you recommend us
something in this matter as well?



Best regards,


Dr Grzegorz Tylko
Department of Cytology and Histology
Institute of Zoology
Jagiellonian University
ul. Ingardena 6
30-060 Krakow
fax: +48-12-634-49-51
phone: +48-12-633-63-77 ext. 2425
mobile phone: +48-602-535-041
e-mail: tylko-at-zuk.iz.uj.edu.pl


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From: rothbardd-at-netscape.net
Date: Fri, 30 Sep 2005 11:38:26 -0500
Subject: [Microscopy] RE: vacuum pumps and LaB6

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tylko,

I would say a replacement of DP to a turbo molecular pump is a good choise
as long as you could find a way to isolate the vibration and modify the
circuit. For Turbo Pump, I would suggest purchase the pump Balzer simply
because you may expect longer lifetime. Wish this will help.

Best Regards,
----------------------------------------------
Xiang Yang
Electron Microscopy Center
Faculty of Graduate Studies and Research
Saint Mary's University
Science Building, Suite 007 and 101
923 Robie Street
Halifax, NS Canada B3H 3C3
Tel: (902) 496-8292 (lab)
(902) 420-5709 (off)
Email: xiang.yang-at-smu.ca


----- Original Message -----
X-from: {tylko-at-zuk.iz.uj.edu.pl}
To: {xyang-at-SMU.CA}
Sent: Friday, September 30, 2005 3:14 AM

If you don't already have a foreline trap, I recommend added one. They are cheap, simple, and very effective in diminishing backstreaming. In the past I have used some from M.E. Taylor (www.semsupplies.com), but there are many others available.

The ion-pumped gun with LaB6 will cause little or no improvement in backstreaming. LaB6 is inherently more unstable than tungsten. It's normally not a problem, or even noticeable, unless you are (as you mention) doing quantitative EDS, or even quantitative time-dependent image analysis.

Good luck,

David Rothbard
Bureau of Engraving & Printing
Washington, DC

tylko-at-zuk.iz.uj.edu.pl wrote:

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From: tivol-at-caltech.edu
Date: Fri, 30 Sep 2005 13:15:01 -0500
Subject: [Microscopy] Re: viaWWW: preparing coated grids for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Sep 29, 2005, at 5:22 PM, fulton.2-at-osu.edu wrote:

} Question: We have been preparing coated grids for TEM for a long time,
} with relatively little trouble. We follow the protocol for preparation
} of formvar coated grids from the Bozzola and Russel text, Electron
} Microscopy. I recently tried to prepare grids with very little
} success. I was using fresh formvar solution from EMS, thinking that
} our old solution might be the problem. I tried several different
} brands of slides as well, all leading to no success. Have any of you
} out there experienced similar problems? Any ideas would be greatly
} appreciated. You may reply offline if you wish. Thank you.
}
Dear Dave,
There are some subtle environmental conditions that can affect your
success rate. You don't say which step is failing, so I can't be too
specific, but the following have adversely affected me: humidity,
cleanliness of the slides (more is not necessarily better), temperature
of the water bath used to float off the formvar, thickness of the
formvar, freshness of the solvents--especially CHCl3, quality of the
razor blade used to scrape the edges of the slide, and the type of
grease used to facilitate separation of formvar from the slide. I have
even found that nose grease from different people can have different
properties, so if there are new people in your lab, and they are using
nose grease, have them let others donate to see if that changes things.
I have found that Apiazon L makes a suitable grease, and I have
floated films off using .25 g of Alconox in 1 L H2O when I have used
Apiazon.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: jehrman-at-mta.ca
Date: Fri, 30 Sep 2005 13:25:53 -0500
Subject: [Microscopy] viaWWW: preparing coated grids for TEM

Contents Retrieved from Microscopy Listserver Archives
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OK, I've known about and used the nose grease/Formvar thing for years,
but for some reason just
now it struck me as a real goofy "black magic" sort of thing that must
seem pretty weird to non-microscopists.
Innocent people who stumble on to the list must think we vacation at
Hogwarts...

Anway, to add some more eccentricity to the thread, my old boss (Larry
Thurston) swore that after spreading
nose grease on the slide, the best thing to clean the excess off with
was your dirty lab coat. Not a clean one,
heaven forbid, or the slide might not be slippery enough to get the film
to separate. Always worked for me...

Any more potions, charms, spells or jinxes? They're really quite
fascinating.

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf



tivol-at-caltech.edu wrote:

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From: paul_hazelton-at-umanitoba.ca
Date: Fri, 30 Sep 2005 14:00:16 -0500
Subject: [Microscopy] viaWWW: preparing coated grids for TEM

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bill -

1. i've always used dichloroethane for formvar. what advantage would
there be to chloroform - how well does work?

2. ok, i gotta plead ignorance. for delicacy perhaps we should use
apiezon for the example and leave 'nose grease' to our fertile
imaginations. but in 35 years i've never heard of using apiezon to help
get the formvar off the slide for coated grids. i'm deeply intrigued.
enlighten me. enlighten the rest of us. it could be a good trick to
know.

paul



Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926


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From: marc.pypaert-at-yale.edu
Date: Fri, 30 Sep 2005 14:27:45 -0500
Subject: [Microscopy] viaWWW: preparing coated grids for TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi Chris,

I know of a retired scientist who had used sucrose in her research on eyes. The reference is as following:
Yang, W.C., M.J. Hollenberg and J.P.H. Wyse. Morphology of the
retinal pigment epithelium in the vit. A deficient rat. Virchows Arch.
B Cell Path. 27, 7-21 (1978)

The eyes were removed form the animal and punctured at the ora serrata
with a razor blade. They were then fixed by immersion in a mixture of 2%
Paraformaldehyde and 2.5% glutaraldehyde (after Karnovsky, 1965) in 0.1M
Sodium cacodylate buffer, pH 7.3 for 4-5 h. After removal of the
cornea and lens the eyes were then rinsed overnight in 0.1M sodium
cacodylate buffer containing 3% sucrose and post-fixed in 2% osmium
tetroxide in the same buffer for 2 h. at room temperature. The specimens
were dehydrated in a graded alcohol series, cut into smaller pieces with
a razor blade and then transferred to propylene oxide. The specimens
were embedded in Epon 812 which was allowed to polymerize overnight in a
60 C oven.
If you like to have a copy of the paper, she is willing to send you one.

Ann Fook Yang
EM Unit/ Unite EM
AAFC/AAC
960 Carling Ave,
Ottawa,Ontario
Canada K1A 0C6
yanga-at-agr.gc.ca
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
X-from: keith.morris-at-ucl.ac.uk [mailto:keith.morris-at-ucl.ac.uk]
Sent: Friday, September 30, 2005 6:47 AM
To: Yang, Ann-Fook

Hi Chris,

As an institute of ophthalmology I suppose we should be able to help - but
I'm now in the cell biology department where tissues are slightly frowned
apon.

Generally sucrose solutions are regularly used as a cryoprotectant. For
non-cryogenic light microscopy, sucrose immersion is generally a poor choice
for paraffin wax embedding (I always used Spurr's, LR White resins etc. as
my work always involved considerable image analysis). If you use
paraformaldehyde only (rather than with Gluta-aldehyde) some do add sucrose
to stop strange effects (like blebbing in fixed cells) but this again this
may affect the tissue for paraffin wax embedding.

Glutaladehyde is regularly combined with paraformaldehyde, as in Karnovsky's
fixative, to improve things. These fixatives can be used to fix tissues for
paraffin embedding, but the tissues will be quite brittle (which is why
sucrose is occasionally used, and why I used resin embedding). Standard
gluta-aldehyde recipes are used here to fix eyes that are going to be
embedded for TEM and ultra-sectioning.

Glutaraldehyde is a fixative regularly used for electron microscopic
studies, and I always used this as a secondary fixative after initially
inflating and fixing lungs in osmium tetroxide dissolved in Freon FC-80 - a
method that even fixed macrophages in-situ on airways. I used to prepare
samples more in a TEM manner as I required high quality sections for lung
distribution and micro-dosimetry studies (often with CR-39 autoradiographs
coupled with serial stained histology). Once fixed over a few hours, fixed
tissues were then stored refrigerated in cacodylate buffer (that often has a
little sucrose to help with the osmotic pressure). Osmium fixes lipids
rather than just proteins so really helps on section quality although its
highly toxic properties and 'creep' are a real pain (although once its black
it's osmium dioxide and stable - when its toxic it's invisible).

Unfortunately all my relevant research papers and fixation recipes are up in
the attic at home somewhere, so I am writing from memory. My recipes were no
doubt similar to that found on the web today.

Hope this is of some help.

regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk




----- Original Message -----
X-from: {christopher.hayden-at-novartis.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Wednesday, September 28, 2005 12:25 AM

We use 0.8-1% formvar in chloroform with good success.
We dissolve the formvar directly into a new chloroform
bottle - this avoids any contamination from lab glassware
and humidity in the air.
Glass slides are washed in acetone before use. They are
coated with formvar using a film casting devise from
EMS. Before dipping the slides into a water tank to
detach the film, my technician scores the film at the
edges of the slide with a razor blade and then breathes
gently on the slide. This apparently helps the film
to come off in the water.
One problem we have sometimes had is the film coming
off the grids later on. My technicians have recently found out
that this only happens when they use grids that have been
stored for various periods of time after washing and
drying. So we recommend washing the grids directly
before use (at least for nickel).
Hope this helps

Marc


On Sep 30, 2005, at 2:17 PM, tivol-at-caltech.edu wrote:

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} On Sep 29, 2005, at 5:22 PM, fulton.2-at-osu.edu wrote:
}
}
} } Question: We have been preparing coated grids for TEM for a long
} } time,
} } with relatively little trouble. We follow the protocol for
} } preparation
} } of formvar coated grids from the Bozzola and Russel text, Electron
} } Microscopy. I recently tried to prepare grids with very little
} } success. I was using fresh formvar solution from EMS, thinking that
} } our old solution might be the problem. I tried several different
} } brands of slides as well, all leading to no success. Have any of you
} } out there experienced similar problems? Any ideas would be greatly
} } appreciated. You may reply offline if you wish. Thank you.
} }
} }
} Dear Dave,
} There are some subtle environmental conditions that can affect
} your
} success rate. You don't say which step is failing, so I can't be too
} specific, but the following have adversely affected me: humidity,
} cleanliness of the slides (more is not necessarily better),
} temperature
} of the water bath used to float off the formvar, thickness of the
} formvar, freshness of the solvents--especially CHCl3, quality of the
} razor blade used to scrape the edges of the slide, and the type of
} grease used to facilitate separation of formvar from the slide. I
} have
} even found that nose grease from different people can have different
} properties, so if there are new people in your lab, and they are using
} nose grease, have them let others donate to see if that changes
} things.
} I have found that Apiazon L makes a suitable grease, and I have
} floated films off using .25 g of Alconox in 1 L H2O when I have used
} Apiazon.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
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From: wesaia-at-iastate.edu
Date: Fri, 30 Sep 2005 16:37:57 -0500
Subject: [Microscopy] Re: vacuum pumps and LaB6

Contents Retrieved from Microscopy Listserver Archives
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You did not say what sort of life you are getting out of your W filaments.
That is probably critical to know.

We have an older JEOL 840A SEM with a tungsten gun. We changed our
operating procedure some time ago because of problems with short filament
life ( {30 hours). We decreed that only lab staff would saturate the
filament. Users would only turn on and off the high voltage. The scope
would ramp up the filament current in about 3 seconds and it apparently
caused no great thermal shock to the filament. Our lifetimes improved to
around 100 hours. That indicated to us that users were not saturating
properly or carefully. It worked as long as casual users were all using the
same accelerating voltage. Now, users are changing the voltage on a regular
basis and we are again having to train them how to saturate the filament
for themselves. I am afraid our filament life will again drop.

You can also try setting the filament further back from the wehnelt cap. It
will reduce you emission, but filament lifetime should go up. Steve Chapman
will probably comment that such practice is a little misguided. If we want
performance out of our scopes, it is necessary to push the filament. I try
to balance the two effects. I want good brightness, but I don't like
changing a filament more than about every 100 hours. You should find some
emission current that provides the lifetime you want.

Warren

At 01:11 AM 09/30/05, you wrote:

} Dear Colleagues,
}
} My department would like to improve the vacuum system in our old scanning
} electron microscope JEOL JSM 5410, which is equipped with Si(Li) EDS
} detector. The main reason for this step is to prolong the working time of
} the tungsten filament and/or replace this type of the source with LaB6. We
} would like to diminish oil contamination coming from roughing and diffusion
} pumps as well. We have got two ideas concerning this matter. One option is
} to attach ion pump to the existing pumping system to improve vacuum to 10-7
} mBarr and the second option is to remove diffusion pump and replace it with
} turbomolecular pump. My question is the following: What are advantages and
} disadvantages of such upgrade of the vacuum system? What kind of pump should
} be installed to our microscope? Have you got any experience with these two
} types of pumps? Is this modification worth doing?
}
} Another question concerns the electron gun of JEOL JSM 5410. Should we
} change the tungsten emitter into LaB6 if we would like to perform X-ray
} microanalysis? I heard that this type of gun is not recommended for
} quantitative X-ray microanalysis due to its poor stability. It is also not
} so easy to do such modification I suppose... Could you recommend us
} something in this matter as well?
}
}
}
} Best regards,
}
}
} Dr Grzegorz Tylko
} Department of Cytology and Histology
} Institute of Zoology
} Jagiellonian University
} ul. Ingardena 6
} 30-060 Krakow
} fax: +48-12-634-49-51
} phone: +48-12-633-63-77 ext. 2425
} mobile phone: +48-602-535-041
} e-mail: tylko-at-zuk.iz.uj.edu.pl
}
}
} ==============================Original Headers==============================
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From: icmicroanalysis-at-cox.net
Date: Fri, 30 Sep 2005 17:36:03 -0500
Subject: [Microscopy] viaWWW: JEOL Cross section polisher

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Email: icmicroanalysis-at-cox.net
Name: Dave

Title-Subject: [Filtered] JEOL Cross section polisher

Question: Is anyone aware of a service lab that has the JEOL Cross Section Polisher?

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From: ucdstm-at-yahoo.com
Date: Mon, 3 Oct 2005 07:48:56 -0500
Subject: [Microscopy] viaWWW: STM tips

Contents Retrieved from Microscopy Listserver Archives
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I think that you will find that the oil contamination comes almost solely from the rotary
backing pump, not from the DP, so a foreline trap between the DP and the RP will work
very well. It will also be cheaper and much less trouble than a LN2 trap.

You may like to read Wil Bigelow's excellent book on EM vacuum systems.

cheers

rtch


Date sent: Fri, 30 Sep 2005 01:11:52 -0500
To: r.sims-at-auckland.ac.nz
X-from: tylko-at-zuk.iz.uj.edu.pl
Send reply to: tylko-at-zuk.iz.uj.edu.pl

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Email: ucdstm-at-yahoo.com
Name: Christopher Fleming

Organization: University of California, Davis

Title-Subject: [Filtered] MListserver:

Question: What are a few suppliers of high quality PtIr STM tips?

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From: K.venner-at-ion.ucl.ac.uk
Date: Mon, 3 Oct 2005 07:50:12 -0500
Subject: [Microscopy] viaWWW: coating grids etc

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Email: K.venner-at-ion.ucl.ac.uk
Name: Kerrie Venner

Organization: Institute of Neurology UCL London

Title-Subject: [Filtered] MListserver: coating grids etc,

Question: The use of nose grease is news to me in the EM Lab, and will be filed away for future use. However, the use of nose grease is not confined to science; my flute teacher advocated applying it to the little finger on the right hand, to enable rapid and smooth key changes on the lower end of the instrument. Works a treat!

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From: lazarcorp-at-cs.com
Date: Mon, 3 Oct 2005 07:50:54 -0500
Subject: [Microscopy] viaWWW: LFR Mk3 film recorder

Contents Retrieved from Microscopy Listserver Archives
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Email: lazarcorp-at-cs.com
Name: Dennis Lazar

Title-Subject: [Filtered] MListserver:

Question: Is there a way to notify list members that I have a LFR Mk3
film recorder to donate to a university, school, etc. It is
nearly new in original shipping carton. All software and
hardware plus accessories. I do not want to join the list
but can someone post this for me?

Thank you

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From: owenha-at-csd.uwm.edu
Date: Mon, 3 Oct 2005 10:14:39 -0500
Subject: [Microscopy] Re: viaWWW: preparing coated grids for TEM

Contents Retrieved from Microscopy Listserver Archives
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On the topic of getting films to float off of slides.

My advisor taught me to polish new slides directly out of the box with
crumpled up Ross lens tissue, and to run the edge of a different dry slide
(held at about a 45 degree angle) along the edges of the coated slide to
separate the film from the glass when the slide is immersed in water.
Scrape down from the top of each long edge, then twice on the bottom (from
the middle to one side, then from the middle to the other). Breathe on
the film before floating it off. The films almost always come off, even
in class demos, with no hint of foundation makeup-contaminated nose grease
- not a concern, I'm sure, for all members of the list ;)

You need to rub all the way out to the edges of the flat top and bottom of
the slide with the lens tissue carefully (slide edges are sharp). I've
always used Corning brand slides, so I don't know whether there's
something special about them. I have no commercial interests in Ross lens
tissue, Corning slides, or Clinique, except as a satisfied user.

Heather Owen


Dr. Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
Lapham Hall
3209 N. Maryland Ave.
Milwaukee, WI 53211
USA

Phone: (414)229-6816




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From: stranen_connelly-at-yahoo.com
Date: Mon, 3 Oct 2005 10:30:44 -0500
Subject: [Microscopy] Looking for manuals & documents for Amray 1000A

Contents Retrieved from Microscopy Listserver Archives
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Victoria,
I have access to the manuals that you requested and
will contact you off-List.
The SEM is located at the Univ. of Pennsylvania there
is a chance that it may be
de-commissioned but that is not certain. If it does,
then the part you wish may be available also.
Pat Connelly

vfink-at-shaw.ca wrote:
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submitted by (vfink-at-shaw.ca) from
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------------------------------------------------
Email: vfink-at-shaw.ca
Name: Victoria Fink
Organization: N/A
Title-Subject: [Filtered] MListserver: Looking for
manuals & documents
for Amray 1000A SEM

I am looking for the manuals, or copies of any
documents ( electrical
schemes, etc.) for Amray 1000A SEM? My friend bought
used one. Also, he
is trying to find, and interested to purchase the
electron gun for this
instrument. Thank you in advance for your kind advise,
and any help,
information regarding to this matter.



__________________________________
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From: stranen_connelly-at-yahoo.com
Date: Mon, 3 Oct 2005 11:30:26 -0500
Subject: [Microscopy] substrate for cell culture

Contents Retrieved from Microscopy Listserver Archives
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I have used the Thermonox line of cover slips, and
there is no problem using Ethanol or Acetone for
dehydration or Propylene oxide and I have grown many
types of cells on them and so far all have been very
happy.

For face on cutting I always cut the bottom of the
cell layer first, choosing an area that was more dense
than is usually viewed at LM level - there are more
cells/section and it is easy to see the cell membranes
at TEM level if one needs to know which cell he is in.

To eliminate the plastic of the coverslip I trim so
that I catch an edge of the boundry between the
coverslip and the embedding epon with a razor blade or
the corner of a glass knife and this popps off the
plastic leaving a shiny surface ready to be sectioned.
Next I align the diamond knife NEARLY parallel so that
I do not section the whole face, just most of it.
That way if I make a little mistake in judgement,
there is still a lot of material to section and the
sections show a variety of depths of the cells with
some at the bottom surface while others show the
gradual levels of middle to surface depending on the
tilt of the block.
Many serial sections can be picked up on the same grid
if just the bottom edge of the block is sectioned for
the sections will be very thin from top to bottom but
can be as wide as one is comfortable with.

For cross section results one can use a sandwich type
of embedding by putting 2 pieces of coverslips into a
mold with the cell layers facing eachother. Trim into
the coverslips on both sides with a glass knife
(better control of depth) and the sections can be
nearly two mm in length but very narrow. This is
similar to a re-embedding technique that I presented
at M&M '77 and is published in Hyatt's TEM books.

If you do have a Hayett - PLEASE make a correction for
future reference...in working with TC cells grown in
standard plastic petri dishes or flasks like Falcon,
Costar, etc. several epon substitutes will melt the
plastic especially when in the oven so cross out the
word "Epon" and replace it with "LX-112 (Ladd) or
similar substitute". Most of you already know about
this but for beginners it can ruin an experiment if
followed as written. At the time that I presented the
paper I was still using the original epon from Shell
and was unaware that the several substitutes had
different properties until about 10 years ago when I
needed to use the technique again and melted a few
dishes!

Pat Connelly
(unemployed in Pennsylvania)

--- TindallR-at-missouri.edu wrote:
} One commonly used substrate is the Thermonox line of
} cover slips,available from many suppliers. They
come in various sizes and the cells can be grown on
them, then the coverslips with cells fixed, dehydrated
and embedded as usual. The section quite well, except
for an unfortunate tendency for sections not to detach
completely in the boat and to pull the next section
back over the edge of the knife. This can be remedied
by making a pointy block face or by using a razor
blade to make a line just beneath the top of the block
face so the section detaches just before the end of
the cutting stroke.

The cover slips can be cut into pieces small enough
to embed in ordinary capsules and are normally used to
do cross-sections through cell layers, as opposed to
"top down" sections. With care and creativity you
should also be able to get other orientations, but it
would be much trickier.

} I'm not 100% sure of their compatibility with PO,
but I don't believe it's a problem. They stand up
fine to EPON and other resins we use.

An alternative is to grow the cells on any old cover
slips, run them through the fixation, dehydration, and
infiltration steps, then place them cell side down on
top of resin filled (with a resin meniscus slightly up
over the top) embedding capsules.
Polymerize them as usual, taking care that they are
level so the cover slips don't slide off.
After everything is mostly hard, immerse the
coverslip/capsule assembly in liquid nitrogen, then
snap off the coverslip from the capsule. If all goes
well, and it usually does, the cells with transfer to
the resin in the capsule and form the top layer of the
block. Identify your area under a light microscope,
trim away the rest (careful!), then start taking
sections. Remember that there is only one cell
layer----if you cut through it and discard it by
mistake, it's gone.

} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu

} -----Original Message-----
} X-from: Ralph.Common-at-hc.msu.edu
} Sent: Thursday, September 29, 2005
} Subject: [Microscopy] substrate for cell culture
}
} A client needs to have cell cultures of endothelial
} cells embedded for TEM. I would be grateful to hear
(off line) specific recommendations
} for a substrate for growing the cells. The
} substrate will have to
} withstand ethanol and propylene oxide, and have good
} sectioning
} properties when used with an Epon type resin. I
} would also appreciate recommendations for fixation.
}
} Ralph Common
} Division of Human Pathology
} Michigan State University





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13, 20 -- From: Pat Conelly {stranen_connelly-at-yahoo.com}
13, 20 -- Subject: Re: [Microscopy] RE: substrate for cell culture
13, 20 -- To: TindallR-at-missouri.edu
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From: tivol-at-caltech.edu
Date: Mon, 3 Oct 2005 11:59:24 -0500
Subject: [Microscopy] viaWWW: preparing coated grids for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Sep 30, 2005, at 12:00 PM, paul r hazelton wrote:

} 1. i've always used dichloroethane for formvar. what advantage would
} there be to chloroform - how well does work?
}
} 2. ok, i gotta plead ignorance. for delicacy perhaps we should use
} apiezon for the example and leave 'nose grease' to our fertile
} imaginations. but in 35 years i've never heard of using apiezon to
} help
} get the formvar off the slide for coated grids. i'm deeply intrigued.
} enlighten me. enlighten the rest of us. it could be a good trick to
} know.
}
Dear Paul,
Our lab in Albany had a recipe that called for dissolving formvar in
equal parts chloroform and acetone. I have also used the pre-made
solution of formvar in dichloroethane, and I haven't found any
particular difference. I don't know which of the two chloro-carbons is
more stable--I suspect DCE--but that would be the preferred solvent.
I tried two methods with apiazon, and had about equal success. I
should say that I was making holey formvar to end up with holey carbon
films, and I was having absolutely no luck getting the films to
separate from the glass slides until I tried the apiazon. The lab's
procedure called for taking pre-cleaned slides and rinsing them in
ethanol, then wiping them dry. They were then made more-or-less
controllably less clean by applying a thin coat of grease, but when the
oil from my skin proved to be deficient, and that from other members of
the lab worked, I decided to try something more well-defined. One
trick for aligning the holes in holey carbon is to apply the grease to
the slide, then rub with one's finger and thumb along the long
direction of the slide. The ridged residual grease will cause the
holes (made by glycerol droplets) to line up along the ridges. When I
first tried apiazon, I just used the same technique as with skin oil,
using as small an amount of apiazon as I could. Later I thought to
make the process even more well-defined by disolving the apiazon, and a
relatively-high-boiling-point petroleum ether (hexanes or heptanes most
likely) proved to be a good solvent. For this procedure, I dissolved a
measured amount of apiazon in 50 ml of solvent, then dipped the slide
and let it drain in the same manner as used for dipping in formvar
solution. In order to try to avoid any residual grease remaining on
the underside of the formvar, I floated the film off with a dilute,
warm detergent solution--0.25 g Alconox in 1 L DDH2O.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



==============================Original Headers==============================
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From: connie.a.cummings-at-gsk.com
Date: Mon, 3 Oct 2005 12:25:17 -0500
Subject: [Microscopy] viaWWW: feedback on JEOL 1010 microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (connie.a.cummings-at-gsk.com) from http://www.microscopy.com/MLFormMail.html on Monday, October 3, 2005 at 10:43:15
---------------------------------------------------------------------------

Email: connie.a.cummings-at-gsk.com
Name: Connie Cummings

Organization: GlaxoSmithKline

Title-Subject: [Filtered] MListserver:

Question: Good day,
I am in need of some information from those folks out there that have a JEOL 1010 microscope? Are you happy with it? Has it had many problems and if so what type of problems? I appreciate any feedback.
Thanks so much.
Connie

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: stmccart-at-vt.edu
Date: Mon, 3 Oct 2005 13:02:00 -0500
Subject: [Microscopy] TEM of partially fluorinated disulfonated poly(arylene ether

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are trying to image the hydrophilic/hydrophobic bulk phase separation
behavior of a series of partially fluorinated disulfonated poly(arylene ether
sulfone) random copolymers using TEM. The copolymers are completely
amorphous and the
only difference between the hydrophilic and hydrophobic sections of their
backbone is that the hydrophilic section possesses two sulfonic acid groups,
each one covalently bonded to one of two arylene rings which flank a sulfone
group. A membrane solvent-cast from one of these copolymers was
quantitatively titrated with cesium hydroxide in an attempt to increase the
difference in electron density between the hydrophobic and hydrophilic
domains. Despite efforts to microtome samples as thin as possible (~50 nm),
little to no contrast was observed in the micrographs of these samples imaged
by TEM at 100kV. We would appreciate any suggestions for alternative staining
techniques for these copolymers.


Stephen McCartney
Senior Research Associate
Macromolecules and Interfaces Institute
2108 Hahn Hall
Va Tech
Blacksburg, VA 24061
540-231-9765 - phone
540-231-8517 - FAX


==============================Original Headers==============================
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4, 21 -- Subject: TEM of partially fluorinated disulfonated poly(arylene ether
4, 21 -- sulfone) random copolymers
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From: ladres-at-worldnet.att.net
Date: Mon, 3 Oct 2005 15:13:02 -0500
Subject: [Microscopy] need a microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We need an American Optical Model 27K Stereo Scope with an 8x objective. If
we can't get the full microscope then we can take just the 8x objective.
If any knows where we can get one please let me know.

Thank you,

Ron Peck

Ladd Research
83 Holly Court
Williston, VT 05495

On-line Catalog: http://www.laddresearch.com

tel: 1-802-658-4961(anywhere) or 1-800-451-3406(US)
fax: 1-802-660-8859
e-mail: ladres-at-att.net




==============================Original Headers==============================
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From: wpchan-at-u.washington.edu
Date: Mon, 3 Oct 2005 16:03:54 -0500
Subject: [Microscopy] CPD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I have a question about one of the steps in CPD using ethanol and CO2.
We have both a Denton DCP-1 and a Bomer 900EX. I was taught that I should
never let the sample exposed to air after passing the higher alcohol
concentrations e.g., 75% and above. When transferring the dehydrated
sample from the ethanol into the CPD chamber, I will fill the chamber with
enough ethanol to submerge the prep and then transfer the sample
basket/container quickly into the CPD chamber. However, I have come
across quite a few protocols that either do not specify this or simply
fill the chamber with CO2 "snow".

So my question is, should I fill the chamber with ethanol? Am I being
overly cautious or have I missed anything critical (no puns intended!)?
Can this be sample specific e.g., smaller or delicate samples may be
more prone to surface tension disruption? Any advice and comments are
much appreciated.

--
Pang (Wai Pang Chan, wpchan-at-u.washington.edu)

==============================Original Headers==============================
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From: ian-at-emu.usyd.edu.au
Date: Tue, 4 Oct 2005 00:57:11 -0500
Subject: [Microscopy] SEM 505 Monitor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone know who manufactures the monitors used on a Philips SEM505
and/or
whether or not they are obtainable 3rd party?


Dr Ian J Kaplin
Manager, Scanning Electron Microscopy

Electron Microscope Unit
The University of Sydney
NSW 2006
Australia

TEL) + 61 2 9351 3302
FAX) + 61 2 9351 7682
ian.kaplin-at-emu.usyd.edu.au
www.emu.usyd.edu.au
Madsen Building F09
Room LG21

ACMM (19) Sydney 2006:
http://www.acmm19.org.au/


==============================Original Headers==============================
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From: bjulies-at-uwc.ac.za
Date: Tue, 4 Oct 2005 03:59:49 -0500
Subject: [Microscopy] 23 year old Hitachi X650

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Anybody have a 23 year old Hitachi X650 SEM standing around gathering
dust? We, in Cape Town, South Africa, are in desperate need of spares. I
would be really glad if you could help.

Thanks

Basil



Dr. Basil Julies
Director
Electron Microscope Unit
Physics Department
University of the Western Cape
Private Bag X17
Bellville 7535
Tel : (27)(21) 959 2327 or 959 3458
Fax : (27)(21) 959 1335 or 959 3474

==============================Original Headers==============================
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From: cornheadorama-at-hotmail.com
Date: Tue, 4 Oct 2005 07:57:54 -0500
Subject: [Microscopy] viaWWW: High Vacuum Sealing Wax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't wish to turn this into a nose grease forum, but I did come
across nose grease as a suitable emergency photographic film scratch
remover. I believe it was an old journalist/photographer trick for
those times when small scratches needed to be reduced in the darkroom.

I have tried this and it does work, but I would only ever use it for
fine scratches on the back of film (not the emulsion side). If it's a
valuable negative it might be safer to use something more proprietary.

I wonder if any of the e.m. supply companies has considered putting
artificial nose grease onto their catalogues.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
University of Sunderland
Tyne & Wear
SR1 3SD
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: K.venner-at-ion.ucl.ac.uk

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cornheadorama-at-hotmail.com) from http://www.microscopy.com/MLFormMail.html on Monday, October 3, 2005 at 20:51:14
---------------------------------------------------------------------------

Email: cornheadorama-at-hotmail.com
Name: Jeff Miller

Title-Subject: [Filtered] MListserver: High Vacuum Sealing Wax?

Question: Can anyone recommend some low vapor pressure sealing or adhesive waxes or putties for semi-permanent sealing of high vacuum leaks, and maybe to seal mis-matched flanges under compression: that kind of thing. Probably a pair of waxes with melting points of about 60C and 100C or more would help.

Obviously they should have good adhesion properties, preferrably at low temps as well. I'd expect that a small amount of flexibility would be helpful. Best if it has no colorant or secret ingredients.

I'm thinking some of the materials used in mouting specimens might work.

I haven't tried any waxes yet. I've checked specs on a few Apiezon waxes but they seem to have higher vapor pressures than their greases.

Vacseal seems too permanent for most of what I had in mind.

So far I've tried Duxseal, but it has an odor which I can't imagine is good for hi-vac. And sure enough it will cavitate and spring a leak: if you apply it, squeeze tight, and "remove" it you will often get a very good seal that leaks again a few hours later.

Critoseal has no odor and is mostly PVC, I'll be trying that and probably analyzing it for weight loss under vacuum soon. I'm wondering if there are some plumber's putties to consider, there might be some gimmicky teflon putty that could be just the ticket. I was recently gifted 1 oz. of amorphous Teflon powder, which I might try mixing with some heavy Apiezon greases.

-Jeff


---------------------------------------------------------------------------

==============================Original Headers==============================
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13, 12 -- Subject: viaWWW: High Vacuum Sealing Wax?
13, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: mike.young-at-yale.edu
Date: Tue, 4 Oct 2005 08:28:18 -0500
Subject: [Microscopy] Re: viaWWW: High Vacuum Sealing Wax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The old remedy I recall from the Dark Ages is: "glyptal". It was
originally marketed (by GE?) as an anti-corona goop , but soon
thereafter someone discovered it had surprisingly good vacuum sealing
properties as well.

Does anyone know if it's still available?

--Mike Young
mike.young-at-yale.edu

cornheadorama-at-hotmail.com wrote:

}
}
} Title-Subject: [Filtered] MListserver: High Vacuum Sealing Wax?
}
} Question: Can anyone recommend some low vapor pressure sealing or adhesive waxes or putties for semi-permanent sealing of high vacuum leaks, and maybe to seal mis-matched flanges under compression: that kind of thing. Probably a pair of waxes with melting points of about 60C and 100C or more would help.
}
} {snip}
}
}


==============================Original Headers==============================
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6, 22 -- MIME-Version: 1.0
6, 22 -- To: microscopy list {microscopy-at-microscopy.com}
6, 22 -- Subject: Re: [Microscopy] viaWWW: High Vacuum Sealing Wax?
6, 22 -- References: {200510041305.j94D57ZL023447-at-ns.microscopy.com}
6, 22 -- In-Reply-To: {200510041305.j94D57ZL023447-at-ns.microscopy.com}
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6, 22 -- Content-Transfer-Encoding: 7bit
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==============================End of - Headers==============================




From: ab78-at-esc.cam.ac.uk
Date: Tue, 4 Oct 2005 08:41:10 -0500
Subject: [Microscopy] Re: glyptal... Re: viaWWW: High Vacuum Sealing Wax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

http://www.caswellplating.com/aids/glyptal.html


mike.young-at-yale.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
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} The old remedy I recall from the Dark Ages is: "glyptal". It was
} originally marketed (by GE?) as an anti-corona goop , but soon
} thereafter someone discovered it had surprisingly good vacuum sealing
} properties as well.
}
} Does anyone know if it's still available?
}
} --Mike Young
} mike.young-at-yale.edu
}
} cornheadorama-at-hotmail.com wrote:
}
}
} }
} } Title-Subject: [Filtered] MListserver: High Vacuum Sealing Wax?
} }
} } Question: Can anyone recommend some low vapor pressure sealing or adhesive waxes or putties for semi-permanent sealing of high vacuum leaks, and maybe to seal mis-matched flanges under compression: that kind of thing. Probably a pair of waxes with melting points of about 60C and 100C or more would help.
} }
} } {snip}
} }
} }
}
}
}
} ==============================Original Headers==============================
} 6, 22 -- From mike.young-at-yale.edu Tue Oct 4 08:28:18 2005
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} 6, 22 -- From: Mike Young {mike.young-at-yale.edu}
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} 6, 22 -- Subject: Re: [Microscopy] viaWWW: High Vacuum Sealing Wax?
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}

--

Andy Buckley

AB78-at-ESC.CAM.AC.UK
DEPARTMENT OF EARTH SCIENCES
UNIVERSITY OF CAMBRIDGE Tel +44 1223 333469
DOWNING STREET +44 1223 333400
CAMBRIDGE FAX +44 1223 333450
CB2 3EQ

Manager of XRD discussion list:
http://www.jiscmail.ac.uk/lists/xrd.html

==============================Original Headers==============================
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From: jbs-at-temple.edu
Date: Tue, 4 Oct 2005 09:40:00 -0500
Subject: [Microscopy] History - Van Leeuwenhoek Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I was re-reading some catalogues of exhibits of Van Leeuwenhoek
microscopes, and I was struck, as usual, by the description of the
glass lenses that he made for his observations. These descriptions
reminded me of a comment that I overheard in passing at a recent M&M
meeting that Van Leeuwenhoek also made (or used) lenses made of
droplets of water. I know that he made a "water microscope", but I
always understood this to mean that the sample was liquid, contained
in a glass tube. Can anyone provide verification of the use of water
as a lens?

Joel




Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


==============================Original Headers==============================
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8, 19 -- From: "Joel Sheffield" {jbs-at-temple.edu}
8, 19 -- To: microscopy-at-microscopy.com
8, 19 -- Date: Tue, 04 Oct 2005 10:37:58 -0400
8, 19 -- MIME-Version: 1.0
8, 19 -- Subject: History - Van Leeuwenhoek Lenses
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From: pgrover-at-bilbo.bio.purdue.edu
Date: Tue, 4 Oct 2005 10:21:56 -0500
Subject: [Microscopy] water lens van Leeuwenhoek microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Joel,

Check out

http://www.microscopy-uk.net/mag/indexmag.html?http://www.microscopy-uk.net/
mag/art98/watermic.html

I haven't made a water drop microscope, but I have been making Leeuwenhoek
glass ball microscope replicas. I haven't yet honed my lensmaking skills to
resolve bacilli, spirilla, cocci, like he did, but diatoms look awesome
through it! I just twirl a glass thread in a flame to make the spheres, but
I don't think I have the patience to do the polishing that he did. I may
cheat and buy one of the Edmund Optics glass ball lenses (~$20).

Paul Grover


The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hi all,

I was re-reading some catalogues of exhibits of Van Leeuwenhoek
microscopes, and I was struck, as usual, by the description of the
glass lenses that he made for his observations. These descriptions
reminded me of a comment that I overheard in passing at a recent M&M
meeting that Van Leeuwenhoek also made (or used) lenses made of
droplets of water. I know that he made a "water microscope", but I
always understood this to mean that the sample was liquid, contained
in a glass tube. Can anyone provide verification of the use of water
as a lens?

Joel




Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs






==============================Original Headers==============================
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18, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu}
18, 24 -- To: {microscopy-at-microscopy.com}
18, 24 -- Subject: water lens van Leeuwenhoek microscopes
18, 24 -- Date: Tue, 4 Oct 2005 10:21:55 -0500
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From: pgrover-at-bilbo.bio.purdue.edu
Date: Tue, 4 Oct 2005 10:36:50 -0500
Subject: [Microscopy] re: water drop microscope - p.s.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For some reason, if you just click on the url, the last part of it doesn't
copy, and you won't get all the way to the web page. You may have to cut 'n
paste the last part, or type it in, but it's worth the trouble!


Joel,

Check out

http://www.microscopy-uk.net/mag/indexmag.html?http://www.microscopy-uk.net/
mag/art98/watermic.html

I haven't made a water drop microscope, but I have been making Leeuwenhoek
glass ball microscope replicas. I haven't yet honed my lensmaking skills to
resolve bacilli, spirilla, cocci, like he did, but diatoms look awesome
through it! I just twirl a glass thread in a flame to make the spheres, but
I don't think I have the patience to do the polishing that he did. I may
cheat and buy one of the Edmund Optics glass ball lenses (~$20).

------------------------------------------------------------------------
May your trails be crooked, winding, lonesome, dangerous, leading to the
most amazing view.

- Edward Abbey




==============================Original Headers==============================
10, 24 -- From pgrover-at-bilbo.bio.purdue.edu Tue Oct 4 10:36:50 2005
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10, 24 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu}
10, 24 -- To: {microscopy-at-microscopy.com}
10, 24 -- Subject: re: water drop microscope - p.s.
10, 24 -- Date: Tue, 4 Oct 2005 10:36:49 -0500
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From: keith.morris-at-ucl.ac.uk
Date: Tue, 4 Oct 2005 10:43:40 -0500
Subject: [Microscopy] viaWWW: High Vacuum Sealing Wax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Do you mean glyptal red enamel ? - the automotive stuff that is used to seal
glass coverslips to slides like high performance nail varnish (for cleared
insect bodies etc...). It's a hard [cellulose derived polyester alkyd resin]
paint you see on dynamo's & other electric machinery and some engines (and
it seals up pores in engine blocks). Glyptal was made by General Electric
(who coined the name in the 20's). If so try:

http://onlinesaleshop.biz/eastwood-product-2591.html (or the link I notice
has just been given )

http://www.glyptal.com/ is down at the moment for some reason but they do
make other types (I'm sure black at least).

I'm sure I've have seen it somewhere as a clear varnish that may suit
better, as a re-inforcement for cloth mouldings. I think glyptal paint is
normally baked in a curing oven when on metal (which makes it rather
permanent should things go wrong).

Sam's laser facts suggests using Glyptal red for vacuum sealing : "Anyhow,
once the whole affair was drenched in Red Glyptal, leaks really weren't an
issue!" :
http://weber.ucg.ie/mirrors/www.repairfaq.org/sam/lasercon.htm (search for
Glyptal)
so I suppose it must be OK.

Originally clear Glyptal resin was used similarly to shellac (e.g. as a hard
coating/glue). It ages badly though going red tinged over the years (eg. on
fossils). You can use acetone to remove Glyptal when used as a sealant on
slides (but its not very easy), but I'm not sure it will be at all easy if
bake cured onto metal.

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


==============================Original Headers==============================
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10, 27 -- From: "Keith J Morris" {keith.morris-at-ucl.ac.uk}
10, 27 -- To: {Microscopy-at-microscopy.com}
10, 27 -- Subject: [Microscopy] viaWWW: High Vacuum Sealing Wax?
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From: jbs-at-temple.edu
Date: Tue, 4 Oct 2005 11:06:23 -0500
Subject: [Microscopy] water drop microscope - p.s.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you very much. I enjoyed the references. It's an amusing idea
to use the biological lens as a microscope lens. So, did Van
Leeuwenhoek himself use one of these?


BTW, the URL that finally worked was:

http://www.microscopy-uk.net/mag/art98/watermic.html

When I entered this, the web site generated the full URL. Odd.

}
}
}
} ----------------------------------------------------------------------
} ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} ------
}
} For some reason, if you just click on the url, the last part of it
} doesn't copy, and you won't get all the way to the web page. You may
} have to cut 'n paste the last part, or type it in, but it's worth the
} trouble!
}
}
} Joel,
}
} Check out
}
} http://www.microscopy-uk.net/mag/indexmag.html?http://www.microscopy-u
} k.net/ mag/art98/watermic.html
}
} I haven't made a water drop microscope, but I have been making
} Leeuwenhoek glass ball microscope replicas. I haven't yet honed my
} lensmaking skills to resolve bacilli, spirilla, cocci, like he did,
} but diatoms look awesome through it! I just twirl a glass thread in a
} flame to make the spheres, but I don't think I have the patience to do
} the polishing that he did. I may cheat and buy one of the Edmund
} Optics glass ball lenses (~$20).
}
} ----------------------------------------------------------------------
} -- May your trails be crooked, winding, lonesome, dangerous, leading
} to the most amazing view.
}
} - Edward Abbey
}
}
}
}
} ==============================Original
} Headers============================== 10, 24 -- From
} pgrover-at-bilbo.bio.purdue.edu Tue Oct 4 10:36:50 2005 10, 24 --
} Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu
} [128.210.5.129]) 10, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with
} ESMTP id j94FanLt027960 10, 24 -- for {microscopy-at-microscopy.com} ;
} Tue, 4 Oct 2005 10:36:49 -0500 10, 24 -- Received: from paklabpgrover
} (dhcp155-241.bio.purdue.edu [128.210.155.241]) 10, 24 -- by
} mailhub129.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id
} j94FanN4011651 10, 24 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct
} 2005 10:36:49 -0500 10, 24 -- From: "pgrover"
} {pgrover-at-bilbo.bio.purdue.edu} 10, 24 -- To:
} {microscopy-at-microscopy.com} 10, 24 -- Subject: re: water drop
} microscope - p.s. 10, 24 -- Date: Tue, 4 Oct 2005 10:36:49 -0500 10,
} 24 -- Message-ID: {000001c5c8f9$6ff70a00$f19bd280-at-paklabpgrover} 10,
} 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; 10, 24 --
} charset="us-ascii" 10, 24 -- X-Priority: 3 (Normal) 10, 24 --
} X-MSMail-Priority: Normal 10, 24 -- X-Mailer: Microsoft Outlook, Build
} 10.0.4510 10, 24 -- Importance: Normal 10, 24 -- X-MimeOLE: Produced
} By Microsoft MimeOLE V6.00.2900.2180 10, 24 -- X-PMX-Version:
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} quoted-printable to 8bit by ns.microscopy.com id j94FanLt027960
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} Headers==============================


Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


==============================Original Headers==============================
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9, 20 -- From: "Joel Sheffield" {jbs-at-temple.edu}
9, 20 -- To: pgrover-at-bilbo.bio.purdue.edu, microscopy-at-microscopy.com
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From: edelmare-at-muohio.edu
Date: Tue, 4 Oct 2005 11:09:06 -0500
Subject: [Microscopy] Re: CPD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Pang:

I think your being a little overly cautious - which should not hurt,
but may take longer in CO2 flushing to remove (plus sends a lot of
EtOH out the CPD exhaust to evaporate which generally appears
be against most chemical waste protocols, which I will not argue
either way)

In my experience the rule is: Always keep the samples wetted,
prevent "air drying". We usually process CPD samples inside of
little containers (metal baskets or scintered teflon, i.e. marshmallow
baskets, or coverslip racks). We load the samples into the
containers under 100% EtOH, and then transfer the containers to
the CPD chamber. Alot of EtOH gets transfered with the samples
into the CPD chamber. (To test this place a basket into EtOH,
carefully remove it from the EtOH with forceps, and then give it a
hard shake over a counter top. You will see alot of EtOH coming
from the basket). This EtOH carries over long enough for us to
replace the CPD chamber lid, tighten down the retaining bolts, and
crack open the CO2 fill valve. As soon as some CO2 hits the
chamber: (1) the expanding gas cools the chamber atmosphere,
(2) rapidly increases the chamber pressure, and (3) starts wetting
the samples with CO2. The first two conditions slow and stop the
vaporization of the EtOH, and the third wets the sample with a new
solution.

As I tell my students the only "rush" period in CPD preparation
is from the moment the sample baskets are removed from the
100% solvent and the CO2 fill valve is cracked open (cracked open
slowly to prevent throwning the sample basket(s) around inside the
CPD chamber as the CO2 rushes into fill the chamber.

And as a followup to this: Never allow the CO2 level to fall
below the sample height during the CO2 flushes. Until you are
transitioning to the critical point (in which case you are also dealing
with a saturated liquid CO2 environment anyway).

On 3 Oct 2005, at 16:04, wpchan-at-u.washington.edu wrote:

}
} Hi
}
} I have a question about one of the steps in CPD using ethanol and CO2.
} We have both a Denton DCP-1 and a Bomer 900EX. I was taught that I should
} never let the sample exposed to air after passing the higher alcohol
} concentrations e.g., 75% and above. When transferring the dehydrated
} sample from the ethanol into the CPD chamber, I will fill the chamber with
} enough ethanol to submerge the prep and then transfer the sample
} basket/container quickly into the CPD chamber. However, I have come
} across quite a few protocols that either do not specify this or simply
} fill the chamber with CO2 "snow".
}
} So my question is, should I fill the chamber with ethanol? Am I being
} overly cautious or have I missed anything critical (no puns intended!)?
} Can this be sample specific e.g., smaller or delicate samples may be
} more prone to surface tension disruption? Any advice and comments are
} much appreciated.
}
} --
} Pang (Wai Pang Chan, wpchan-at-u.washington.edu)
}


Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"WE ARE MICROSOFT.
RESISTANCE IS FUTILE.
YOU WILL BE ASSIMILATED."

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From: rcbaker-at-eden.infohwy.com
Date: Tue, 4 Oct 2005 11:26:45 -0500
Subject: [Microscopy] water drop microscope - p.s.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Actually Leeuwenhoek used ground and polished glass lenses, probably
made with a tube lap. He was certainly familiar with "blown" or fused
lenses which, however, usually do not have the accuracy of curvature
required for the best optical performance.

Details on how to grind and polish short focus bead lenses and
various other ways to make homemade microscope objectives with high
resolution are given here:

{http://www.science-info.org/pages/Roger%20Baker/homemade-
microscope.pdf}

Leeuwenhoek's highest resolution discoveries must certainly have been
made with the front lens in wet contact with the specimen, since the
laws of optical physics do not permit the sub-micron resolution
needed to observe bacteria and the correct tail length of
spermatozoa; a one micron resolution is about the best that can be
done with a dry lens.

These conclusions are more fully discussed in my article in "The
Microscope"; "Leeuwenhoek, the Short-focus Lens, and the History of
Optics; an Experimental Inquiry" in the first quarter of 1993.

The known facts seem to indicate that Leeuwenhoek must have both
invented the immersion objective and used a double lens, although he
used and revealed various single lens microscopes too. This explains
a number of historical facts, including Leeuwenhoek's claim that he
did not reveal his best microscopes to others.

-- Roger, Austin



On Oct 4, 2005, at 10:39 AM, pgrover-at-bilbo.bio.purdue.edu wrote:


}
} For some reason, if you just click on the url, the last part of it
} doesn't
} copy, and you won't get all the way to the web page. You may have
} to cut 'n
} paste the last part, or type it in, but it's worth the trouble!
}
}
} Joel,
}
} Check out
}
} http://www.microscopy-uk.net/mag/indexmag.html?http://
} www.microscopy-uk.net/
} mag/art98/watermic.html
}
} I haven't made a water drop microscope, but I have been making
} Leeuwenhoek
} glass ball microscope replicas. I haven't yet honed my lensmaking
} skills to
} resolve bacilli, spirilla, cocci, like he did, but diatoms look
} awesome
} through it! I just twirl a glass thread in a flame to make the
} spheres, but
} I don't think I have the patience to do the polishing that he did.
} I may
} cheat and buy one of the Edmund Optics glass ball lenses (~$20).

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From: cgarber-at-2spi.com
Date: Tue, 4 Oct 2005 11:43:04 -0500
Subject: [Microscopy] Glyptal products

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Further on the discussion on Glyptal, this was once all part of General
Electric, and in 1985, the business unit was spun out of GE and now operates
as


GLYPTAL, INC.
305 Eastern Ave.
Chelsea, Ma 02150
(617) 884-6918
1-800-GLP-1201
FAX (617) 884-8376
E-MAIL billhoag-at-comcast.net

The original products made by GE were formulated to be insulating paints for
electrical applications. I can remember back in my days at the DuPont
Experimental Station, and I am talking about the late 1960's, one of the
technicians (Bob Schatz, who was a "teacher" for more than just a few of us
on this listserver) considered his little bottle of Glyptal liquid to be one
of his most valuable possessions. He used it on the bell jar gaskets when
they developed cracks, among other things. However I don't recall his ever
referring to it as anything other than "Glyptal", even though there is an
entire list of products sold under the trade name Glyptal.

However, with the passing of time, there have emerged better vacuum leak
sealant materials, some having been previously mentioned, such as VacSeal.
Another one but not previously mentioned is CelvaSeal. We ourselves prefer
VacSeal because of its lower viscosity. Both end up being cured silicones
and when one wants to remove them, they won't come off with some magic
solvent. But I would assume that there is some temperature where they would
come off but probably before reaching traditional "bake out" temperatures.

Disclaimer: SPI Supplies offers both VacSeal and CelvaSeal vacuum leak
sealants on the SPI Supplies website (see below).

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================



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From: jpshield-at-uga.edu
Date: Tue, 4 Oct 2005 13:30:40 -0500
Subject: [Microscopy] EM job - material/physical

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Assistant Research Scientist Position in Material
Characterization

The Center for Advanced Ultrastructural Research (CAUR) at
University of Georgia announces an opening for a material
characterization specialist in the field of electron
microscopy and X-ray microanalysis. The CAUR supports seven
major research instruments --- two SEMs, two TEMs, a
multiphoton/confocal microscope (polarization/DIC capable),
an X-ray microtomography unit, and a digital inverted
fluorescence microscope. The center has a large and growing
user community in the physical, material, and geological
sciences. This person will be hired at the level of Research
Assistant Scientist, which is a long-term promotion-track
position.
Candidates should have a publication record and commensurate
skills in materials science, electron diffraction, and
sample preparation of both synthetic and natural materials.
A Ph.D. or equivalent experience in the physical sciences
required.
The individual will be responsible for training and
supervising users for TEM, SEM, X-ray microtomography,
collaboration on research proposals, and will have an
opportunity for conducting and publishing research in their
own area of expertise.
Applicants should submit a curriculum vitae, statement of
research interests, and contact information for three
references to Prof. Charles Keith, Director, Center for
Advanced Ultrastructural Research, 151 Barrow Hall,
University of Georgia, Athens, GA 30602.
chkeith-at-cb.uga.edu. Application review will start on
November 1, 2005. Applications received by that date are
assured consideration. UGA is an Equal
Opportunity/Affirmative Action Institution.

John P. Shields
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602

706-542-4080

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From: fundatel-at-gmail.com
Date: Tue, 4 Oct 2005 14:34:42 -0500
Subject: [Microscopy] Looking for SEM and TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
We are looking for working´s TEM and SEM with manuals to receive it in
donation. Our non profit organization will begin teaching and I+ D
proyects with this equipment
We will pay shipping and handling costs

thanks in advance

Fernando Balducci
President
--
Fundatel
Fundación de Telemedicina
Gualeguaychú 878 Dpto 3
Paraná (ER) - Argentina
TE: +54-(0)343-4221716
www.fundatel.org.ar


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From: Roland.Nitschke-at-biologie.uni-freiburg.de
Date: Tue, 4 Oct 2005 14:50:11 -0500
Subject: [Microscopy] Announcment for Imaging Symposium "From static spots to dynamic proteome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,
the Foundation of the “Freiburg Center for Systems Biology” (ZBSA)
at the Albert-Ludwigs-University of Freiburg will take place on November
3, 2005.

Linked to this event is an


*International Imaging Symposium November 4-5, 2005*


*"From static spots to dynamic proteome visualization and beyond"*

organized by the Life Imaging Center Freiburg and sponsored by DFG, BMBF
and SFBs 388, 505, 592.*
*
List of speakers:

F. Brandizzi, Saskatoon (CDN) W. Denk, Heidelberg (D)
M. Dickinson, Houston (USA) TWJ. Gadella, Amsterdam (NL)
G. Galizia, Konstanz (D) H. Gerdes, Heidelberg (D)
M. Heisler, Pasadena (USA) T. Hirano, Tskuba-Higashi (J)
T. Kerpolla, Ann Arbor (USA) P. Lipp, Homburg (D)
J. Lippincott-Schwartz, Bethesda (USA)
T. Meyer, Stanford (USA) R. Murphy, Pittsburgh (USA)
L. Nedbal, Nove Hrady (CZ) M. Oheim, Paris (F)
H. Okamoto, Wako City (J) R. Pepperkok, Heidelberg (D)
W. Schubert, Magdeburg (D) E. Stelzer, Heidelberg (D)
D. Toomre, New Haven (USA) R. Uhl, Martinsried (D)
F. Wouters, Göttingen (D) W. Zipfel, Ithaca (USA)

Further information at:
http://www.zbsa.uni-freiburg.de/seminar/event.php


If you are interested in the Systems Biology Symposium please look here:
Foundation of the Center for Systems Biology (ZBSA) November 3, 2005
http://www.zbsa.uni-freiburg.de/seminar/events.php

Detailed meeting programs and registration and accomodation
information are available on the web.

We are looking forward to see you in Freiburg.

Roland Nitschke and Klaus Palme

___________________________
Nitschke, Roland Dr.
Life Imaging Center
Institute of Biology
Albert-Ludwigs-University Freiburg
Hauptstr.1
D-79104 Freiburg
Germany
___________________________
E-mail: Roland.Nitschke-at-biologie.uni-freiburg.de
phone: 49-761-2032934 or 2902
fax: 49-761-2032941
http://www.sfb592.uni-freiburg.de/z2/index.html

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From: classiccarguy89-at-aol.com
Date: Wed, 5 Oct 2005 08:16:03 -0500
Subject: [Microscopy] AskAMicroscopist: Aquaspirillium serpens

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Email: classiccarguy89-at-aol.com
Name: Ben

Organization: Hemlock High

Education: 9-12th Grade High School

Location: Hemlock, MI, USA

Question: What is Aquaspirillium serpens?

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From: patricia.correia-at-kcl.ac.uk
Date: Wed, 5 Oct 2005 08:16:33 -0500
Subject: [Microscopy] AskAMicroscopist: measuring the size of salivary glands

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Email: patricia.correia-at-kcl.ac.uk
Name: Patricia Correia

Organization: King's College

Education: Graduate College

Location: London, U.K.

Question: I am interested in measuring the size of salivary glands acini to make some comparisons as to either they are atrophic or not. I have been using tissue samples formaline fixed in a optic microscope with the programme analySIS from "Soft Imaging System" (www.soft-imaging.net), which allows us to contour the area of each acini and then gives its reading. Do you know of any better method of measuring it? The current method I'm using implies a great deal of subjectivity: depending on the tissue section, the criteria to select the acini and so forth.

Thank you for your attention.
Best regards, Patricia Correia

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From: beez1-at-bigpond.com
Date: Wed, 5 Oct 2005 08:17:25 -0500
Subject: [Microscopy] viaWWW: Nikon S-Ke coaxial focusing control microscope

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Email: beez1-at-bigpond.com
Name: Rohan Wighton

Organization: Charles Sturt University

Title-Subject: [Filtered] MListserver:

Question: Hi everyone

I have just become the proud owner of a Nikon S-Ke coaxial focusing control type microscope. It has a nikon phase contrast turret. I have several problems, which I list in order of relative importance:

1. I have no transformer for the lamp, and having looked briefly on the net for somewhere to find and buy a replacement, I am beginning to think I might have trouble. Can anyone suggest a contact for this?

2. The knob used to adjust the distance between the eyepieces seems stuck (although the scope is in excellent condition this does not move at all) Will lubrication do the job? If so, what do I use? If not, do I send the scope to an expert?

3. As you may have guessed by now I am a beginner, my most extensive binocular work having been dissecting flowers for a semester (loved it!) Any advice for me and/or some history on my particular model scope would be much appreciated.

Thankyou for taking the time to read this

Rohan

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From: mcauliff-at-umdnj.edu
Date: Wed, 5 Oct 2005 09:24:54 -0500
Subject: [Microscopy] Re: AskAMicroscopist: measuring the size of salivary

Contents Retrieved from Microscopy Listserver Archives
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Get yourself a copy of "Unbiased Sterology" by C.V. Howard and M.G.
Reed. In order to make an accurate assessment of your 'target' you must
get rid of subjectivity in your sampling.

Geoff


patricia.correia-at-kcl.ac.uk wrote:

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From: a.c.richardson-at-durham.ac.uk
Date: Wed, 5 Oct 2005 10:07:14 -0500
Subject: [Microscopy] TEM: Chromium sputter coating

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Dear All,
We have just taken delivery of a chromium sputter coating unit and I am
attempting to do a risk assessment and having read some of the MDS on
Chromium am now very apprehensive about the toxicity of the fumes and
flakes produced by the target. As I am not a chemist, I am unsure what I
am dealing with. If anyone out there has any advice they would be
willing to share, including things like should it be used in a fume hood
and how to dispose of the 'flakes' of chromium, it would be greatly
appreciated. Thanks in advance,
Christine.

A.C.Richardson
Experimental Officer
School of Biological and Biomedical Science
Centre for Molecular Imaging
University of Durham
Science site
South Rd
Durham
England
E-mail: a.c.richardson-at-durham.ac.uk

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From: gary-at-gaugler.com
Date: Wed, 5 Oct 2005 10:35:59 -0500
Subject: [Microscopy] Re: TEM: Chromium sputter coating

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My best advice is to use it quickly. I do not know about
toxicity but I do know that Cr oxidizes rapidly. So,
be alert to this. I use Pt, Ir and Au/Pd instead of Cr.

gary g.


At 08:10 AM 10/5/2005, you wrote:



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From: M_Jarnik-at-fccc.edu
Date: Wed, 5 Oct 2005 11:08:51 -0500
Subject: [Microscopy] Re: CPD

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Dear Ben,

Spirillum serpens is a spiral-shaped bacteria from the genus Spirillum, all
of which which live in water (hence the aqua bit) except for one odd species
that causes a type of rat-bite fever in humans (Spirillum minus). The term
spirillum is used generally for any corkscrew-like shaped species of
bacteria.

These Spirilla bacteria stain Gram-negative. The Gram stain is a special
stain used with a lot of bacteria mounted on a glass slide. Bacteria are
often classified as being positive (violet stained) or negative (stained
pink), when viewed under a standard light microscope. This 'Gram stain'
result is due to differences in the cell wall of the bacteria and this can
be important medically e.g. it can affect how effective antibiotics are at
killing them.

Spirillum bacteria move by means of tufts of flagella (wavey hairlike
structures) at each end of the cell. Spirillum serpens is sometimes looked
at in schools for bacteria morphology (shape) studies, presumably because it
is relatively harmless and conviniently lives in water (e.g.
http://heathscientific.net/item.asp?id=115 ). The bacteria Spirillum serpens
should look a bit similar to the nasty Spirillum minus, see :
http://www.ulb.ac.be/sciences/biodic/images/bacterie/spirillumminus.jpg
where it is stained Gram negative (pink).

Further info:
Gram stain pictures of bacteria can be seen at
http://www.ncl.ac.uk/dental/oralbiol/oralenv/tutorials/gramstain.htm
It's a bit technical but the pictures show how to stain a slide that a lot
of bacteria dried onto the surface.

Regards,
Keith

PS. I had a look at my copy of the Bioaerosols Handbook, 1995 CRC Press Inc
(I wrote chapter 11 - Modern microscopicial methods) but these spirella
bacteria aren't covered, probably as they live in water (aqua) not the air
(aero) which I was interested in. I've never looked at them under a
microscope, so I can't add much more than the above, but I expect that has
already told as much as you wanted to know about the Spirilla bacteria.

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


----- Original Message -----
X-from: {classiccarguy89-at-aol.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Wednesday, October 05, 2005 2:29 PM

We typically dehydrate our samples (e.g. mouse embryos) in increasing
concentrations of EtOH and then change it to amyl acetate (AAC). This
has three advantages -

a) AAC is less volatile than EtOH - we do not have problems with air
drying samples during transfer
b) supposedly, AAC mixes better with liquid CO2
c) AAC has a specific aroma - it's absence is a good indicator the
sample is ready for CPD.

Michal

edelmare-at-muohio.edu wrote:

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From: kirk-at-UDel.Edu
Date: Wed, 5 Oct 2005 11:34:28 -0500
Subject: [Microscopy] Kodak 4489 TEM film devlopment with Mohr Pro 8

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Dear Microscopy Listserv:

I have a colleague who who like to correspond with anyone who has successfully used a MOHR PRO
8 "ME-42" film processor for Kodak 4489 TEM film. He'd like to know what
settings (temp, transport speed, develop time etc) are best. Thanks in advance if you can help.

Best Regards, Kirk

Kirk J. Czymmek, Ph.D.
Associate Professor
Department of Biological Sciences
University of Delaware
Newark, DE 19713
kirk-at-udel.edu




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From: Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Wed, 5 Oct 2005 11:35:10 -0500
Subject: [Microscopy] looking for used PMT

Contents Retrieved from Microscopy Listserver Archives
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Hi everyone,

I am working on designing a new electron detector for our SEM. Does
anyone have an extra PMT with/without a head amplifier? I'm looking for
one that matches the P47 emission spectrum.

If anyone has one just sitting around and gathering dust, I could use it
for experimenting. I would gladly pay for shipping fees.

Thanks,
Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



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From: walck-at-southbaytech.com
Date: Wed, 5 Oct 2005 11:52:11 -0500
Subject: [Microscopy] re: TEM: Chromium sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is little to worry about. The nasty Cr is Cr in the hexavalent state (+6). The Cr of your taget is metallic with a thin oxide on it. The natural oxide of Cr is Cr2O3 which means that the Cr is in the +3 state and is bound up with the oxide.

The process of sputtering is physical bombardment of the target with Ar ions that removes the atoms from the near surface of the target. The process is line of sight deposition onto your substrate and metallic Cr is being deposited (valence state is 0). That is why you need a rotating and tilting sample to get a uniform and continuos coating on your sample for high resolution imaging in the SEM. I assume that the flakes that you are talking about are flakes in the deposition chamber. If you are getting flakes of material on your chamber walls, you must be putting down very thick coatings or very many coatings. Our IBS/e sputter coater can put down a uniform, continuous coating that is less than 10 Angstroms. Your coater should be capable of doing the same. If you deposit these types of films, it should take a long time before you start to get flakes in your system which is thick coatings that peel from the walls because of high stresses in the films. Regardless, in the vacuum system, what is deposited is the metal Cr and it oxides to Cr2O3 when exposed to air when you open the chamber. When you clean your chamber and there is dust or flakes, wear a mask and discard the cleaned material as you would a heavy metal. There will be no Cr fumes anywhere. Cr will not be present in the pump exhaust.

As Gary Gaughler said in his reply, Cr does oxidize fairly rapidly, so samples coated with it must be run soon after. The natural protective oxide that forms on Cr is about the thickness of the coating that you want on your sample. When you consider how thin the thickness of the coating is and how long they can be exposed to atmosphere the actual oxidation rate is not all that high. South Bay Technology has just introduced the SampleSaver(TM) storage container to help prevent this and maintain sample in an inert atmosphere. It is generally accepted that Cr coatings give the best results, but Ir and W coatings approach the quality of Cr and are less susceptible to complete film oxidation. Pt, Au, and Au-Pd coatings do not give as fine a grain size as the other coatings.

Disclaimer:
South Bay Technology, Inc. manufactures and sells the IBS/e ion sputter coater and etching system and the SampleSave(TM) storage container.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: +1-949-492-2600
FAX: +1-949-492-1499
walck-at-southbaytech.com

-------- Original Message --------
} From: a.c.richardson-at-durham.ac.uk
} Sent: Wednesday, October 05, 2005 11:12 AM
} To: Walck-at-SouthBayTech.com
} Subject: [Microscopy] TEM: Chromium sputter coating
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} ----------------------------------------------------------------------------
}
}
} Dear All,
} We have just taken delivery of a chromium sputter coating unit and I am
} attempting to do a risk assessment and having read some of the MDS on
} Chromium am now very apprehensive about the toxicity of the fumes and
} flakes produced by the target. As I am not a chemist, I am unsure what I
} am dealing with. If anyone out there has any advice they would be
} willing to share, including things like should it be used in a fume hood
} and how to dispose of the 'flakes' of chromium, it would be greatly
} appreciated. Thanks in advance,
} Christine.
}
} A.C.Richardson
} Experimental Officer
} School of Biological and Biomedical Science
} Centre for Molecular Imaging
} University of Durham
} Science site
} South Rd
} Durham
} England
} E-mail: a.c.richardson-at-durham.ac.uk
}
} ==============================Original Headers==============================
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From: rjpalmer-at-dir.nidcr.nih.gov
Date: Wed, 5 Oct 2005 11:57:19 -0500
Subject: [Microscopy] TEM services

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am interested in companies/units that do TEM on a "pay-per-play"
basis. I have a small number of bacterial samples on for which
pretty routine workup and picture-snapping is required. Please
contact me off-list.
--
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 310
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396

==============================Original Headers==============================
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From: fulton.2-at-osu.edu
Date: Wed, 5 Oct 2005 18:35:23 -0500
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Thanks for info on formvar

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (fulton.2-at-osu.edu) from http://www.microscopy.com/MLFormMail.html on Wednesday, October 5, 2005 at 10:05:10
---------------------------------------------------------------------------

Email: fulton.2-at-osu.edu
Name: Dave Fulton

Organization: OSU/OARDC MCIC

Title-Subject: [Filtered] MListserver:

Question: Fellow liststers,

A very grateful thanks to all who took the time to answer my query concerning the production of formvar coated grids. I saved them all for future reference!
The two suggestions that got my Mojo working were using nose grease (my own) on the slide after cleaning it with EtOH and dipping the slide into the formvar solution and immediately withdrawing and drying it (instead of holding it in the solution for 10 sec and then in the vapor for 30 sec).

---------------------------------------------------------------------------

==============================Original Headers==============================
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7, 13 -- grids
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From: spb-at-mwrn.com
Date: Wed, 5 Oct 2005 18:36:13 -0500
Subject: [Microscopy] viaWWW: Need Video of Parasites for National Geographic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (spb-at-mwrn.com) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Wednesday, October 5, 2005 at 12:50:28
---------------------------------------------------------------------------

Email: spb-at-mwrn.com
Name: susanne Pignolet Brandom

Organization: www.microscopy.info

Title-Subject: [Filtered] Need Video of Parasites for National Geographic

Question: Please respond to Video4TV-at-aol.com if you can help.

We are producing a program for National Geographic and are interested in licensing footage of the following:

tapeworm
trichurus
scabies
trachoma.

We apologize for the short notice but we would need to receive broadcast quality footage by this Friday, October 7. If anyone has this footage available for licensing, we would appreciate it!

Thanks so much for your efforts!

Linda Callahan
Creative Differences
11846 Ventura Blvd.
Studio City, CA 91604
(818) 769-7809


---------------------------------------------------------------------------

==============================Original Headers==============================
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From: distall2-at-yahoo.com
Date: Wed, 5 Oct 2005 18:36:46 -0500
Subject: [Microscopy] AskAMicroscopist: microorganisms found with electron microscopes?

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (distall2-at-yahoo.com) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, October 5, 2005 at 10:34:05
---------------------------------------------------------------------------

Email: distall2-at-yahoo.com
Name: masood

Organization: Islamic foundation

Education: 9-12th Grade High School

Location: City, State, Country

Question: what are the latest discovered microorganisms found with electron microscopes?

---------------------------------------------------------------------------

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From: gcc-at-couger.com
Date: Thu, 6 Oct 2005 02:12:55 -0500
Subject: [Microscopy] Re: History - Van Leeuwenhoek Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



jbs-at-temple.edu wrote:
}
----------------------------------------------------------------------------

} Hi all,
}
} I was re-reading some catalogues of exhibits of Van Leeuwenhoek
} microscopes, and I was struck, as usual, by the description
of the
} glass lenses that he made for his observations. These
descriptions
} reminded me of a comment that I overheard in passing at a
recent M&M
} meeting that Van Leeuwenhoek also made (or used) lenses made of
} droplets of water. I know that he made a "water microscope",
but I
} always understood this to mean that the sample was liquid,
contained
} in a glass tube. Can anyone provide verification of the use
of water
} as a lens?
}
} Joel
}
}
}
}
Joel

Roger Barker makes a fairly strong case that Van Leeuwenhoek
made and used compound lenses for some of his drawings that
appear to be both theoretically and practically impossible to
resolve with the detail he drew with a single lens in
www.science-info.org/pages/Roger%20Baker/homemade-microscope.pdf

He shows how he made a complex lens using a single lens of glass
and one of glycerine.

Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org


==============================Original Headers==============================
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From: paul_hazelton-at-umanitoba.ca
Date: Thu, 6 Oct 2005 09:24:24 -0500
Subject: [Microscopy] Van Leeuwenhoek lenses and microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

the discussion of Van Leeuwenhoek lenses raises a question of somewhat
personal interest, since i will probably be looking at retiring from the
paid part of the profession in 7-10 years. hey, it's never too early to
think ahead when you have to drop really broad hints in the department.

some years ago we were looking at gifts for a compatriot who was
leaving. we found one company, a purveyor of fine microscope slides for
the student, which marketed silver Van Leeuwenhoek microscopes. the
name was something like North Carolina Bio something, or South Carolina
something-or-other.

does this ring a bell anywhere? can anyone tell me if such a thing
still exists?

i mean, it's either going to be that or a nice Innu sculpture.

paul




Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926




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From: wesaia-at-iastate.edu
Date: Thu, 6 Oct 2005 10:07:36 -0500
Subject: [Microscopy] Re: Van Leeuwenhoek lenses and microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How about neither North nor South. Try http://www.carolina.com/. It looks
like the place you might be looking for.

Warren

At 09:27 AM 10/06/05, you wrote:

} the discussion of Van Leeuwenhoek lenses raises a question of somewhat
} personal interest, since i will probably be looking at retiring from the
} paid part of the profession in 7-10 years. hey, it's never too early to
} think ahead when you have to drop really broad hints in the department.
}
} some years ago we were looking at gifts for a compatriot who was
} leaving. we found one company, a purveyor of fine microscope slides for
} the student, which marketed silver Van Leeuwenhoek microscopes. the
} name was something like North Carolina Bio something, or South Carolina
} something-or-other.
}
} does this ring a bell anywhere? can anyone tell me if such a thing
} still exists?
}
} i mean, it's either going to be that or a nice Innu sculpture.
}
} paul
}
} Paul R. Hazelton, PhD
} Electron Microscope Unit
} University of Manitoba
} Department of Medical Microbiology

-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking


==============================Original Headers==============================
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From: j_dmello-at-yahoo.com
Date: Fri, 7 Oct 2005 03:20:33 -0500
Subject: [Microscopy] SEM Effects on Ear

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

15 DAY AGO I HAVE LOST HEARING TOTALLY,in both ears.
Was using a "In the Ear"(CIC) Digital Hearing Aid (
with Wax buster)
Drs cannot find anything wrong with me Physicallly(
was admitted to hospital for 4 days for all tests)
While I have started work(just completed 25 yrs
service),medicines are going in, still no improvemnet.
Has been working more regularly, with the Jeol 5400
SEM during the past 3 months.Noticed extra battery
dischage in the aid, and lower hearing capacity.Could
it be a cause, of sudden loss of hearing for the last
3 months?
Any of the MSA participants have had this experience?
Kindly let me know, if there is a way out.
Thanks & Regards,

J.J.D'Mello.



__________________________________
Yahoo! Mail - PC Magazine Editors' Choice 2005
http://mail.yahoo.com

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From: wadowska-at-upei.ca
Date: Fri, 7 Oct 2005 06:56:53 -0500
Subject: [Microscopy] TEM diamond knife

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I was given an old diamond knife. Former user does not know the
specifications of this knife. On it's casing there is an engraving WR
and underneath it MP309. I would like to know what kind of
diamond knife it is (ultrathin, histo etc), is it 35 or 45 degree and
what is it's cutting angle. If anybody can identify it I would be
grateful for the information.
Thanks
Dorota

==============================Original Headers==============================
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From: g.greaves-at-salford.student.ac.uk
Date: Fri, 7 Oct 2005 07:45:49 -0500
Subject: [Microscopy] AskAMicroscopist:TEM of porous silicon samples

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (g.greaves-at-salford.student.ac.uk) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, October 7, 2005 at 05:23:35
---------------------------------------------------------------------------

Email: g.greaves-at-salford.student.ac.uk
Name: Graeme Greaves

Organization: University Of Salford

Education: Undergraduate College

Location: Salford, England

Question: I am trying to find information about the preparation of porous silicon samples to be analysed using a transmission electron microscope. What Techniques are there? any knowledge of papers relating to this would be greatly recieved.

yours faithfully

Graeme Greaves

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: Louise_Harner-at-albint.com
Date: Fri, 7 Oct 2005 08:13:51 -0500
Subject: [Microscopy] advice on stereomicroscopes & cameras?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've been asked by two co-workers to provide purchasing advice for
stereomicroscopes with digital image capture and continuous zoom of about 1
to 6 - ideally with options to increase/decrease magnification ranges.

One co-worker will be looking at opaque samples - usually rough cut to a
few inches thick and sometimes further polished. The structures he's
examining are varied shades of gray and black, thus lighting options,
control, & reproducibility are important.

The other person will be looking at machined metal parts similar to long,
thick needles with barbs on them. He's interested in measuring angles and
other dimensions as well as seeing imperfections on the parts.

I have trinocular Olympus stereomicroscopes with a variety of lighting and
lens options, and I'm happy with them for my research lab use. I've also
checked out other companies' products via the web. But I'd like some
real-world feedback on other stereomicroscopes - any you are thrilled with?
any you would never buy again? any really good values for the money?


What are the current favorites for digital image capture - consumer,
prosumer, & professional level digital cameras? I prefer at least 3
megapixels (more is always acceptable since the images can be downsized).
Any comments on ease of use, quality, value, & satisfaction from recent
purchasers?


Please respond directly to me at "Louise_Harner-at-albint.com" so we don't tie
up the listserver with personal opinions.
Vendor replies welcome, but please give pricing info. I need to get back to
my co-workers by Oct. 14.

Thanks in advance!

- Louise

Louise Harner
Research Microscopist, Albany International Research Co.
777 West Street, P.O. Box 9114, Mansfield, MA 02048
phone: 508-337-9529
Louise_Harner-at-albint.com


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From: lkrupp-at-us.ibm.com
Date: Fri, 7 Oct 2005 09:45:33 -0500
Subject: [Microscopy] polishing tools by Precision TEM, Inc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello listers-

Does anyone have experience with using these tools for cross-sectional TEM
specimen preparation? We are considering purchasing them and I am
interested in opinions on the products. Please respond off-list if you
have any comments for me.

Thank you,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099

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From: daniel.thomas-at-univ-rennes1.fr
Date: Fri, 7 Oct 2005 10:31:50 -0500
Subject: [Microscopy] TEM :recovering replicas from mica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

TEM : PT/C replicas of biological molecules.

I'm having troubles in recovering PT/C replicas on water from freshly
cleaved mica. The carbon film remains sticked to the mica instead of
floating on the water surface. Is there any trick to overcome this trouble ?

Thanks


Daniel

Daniel THOMAS
Interactions Cellulaires et Moléculaires
CNRS, UMR 6026
Université de Rennes 1
Campus de Beaulieu, Bt 13
35042 RENNES Cedex

Tel : 02 23 23 6122
fax : 02 23 23 5048

http://www.sdm.univ-rennes1.fr/












==============================Original Headers==============================
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From: meulia.1-at-osu.edu
Date: Fri, 7 Oct 2005 11:03:12 -0500
Subject: [Microscopy] paraffin sections embedding into plastic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have some paraffin sections on glass slides (silane coated, ems
slides) that we would need to embed into Spurr's resin.

Does anyone have a suggestion on how to separate the glass from the
Spurr's resin blocks? We briefly plunged them into liquid nitrogen,
but we did not get consistent results.

Also, shall we use a different resin?

Thank you.

Tea Meulia


--
***************************************
Tea Meulia
Molecular and Cellular Imaging Center
Ohio State University/OARDC
1680 Madison Ave.
Wooster OH 44691

tel.: 330-263-3836 or -3828
fax: 330-202-3563

http://www.oardc.ohio-state.edu/mcic

*****************************************

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From: tivol-at-caltech.edu
Date: Fri, 7 Oct 2005 11:37:29 -0500
Subject: [Microscopy] Re: TEM :recovering replicas from mica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Oct 7, 2005, at 8:31 AM, daniel.thomas-at-univ-rennes1.fr wrote:

} TEM : PT/C replicas of biological molecules.
}
} I'm having troubles in recovering PT/C replicas on water from freshly
} cleaved mica. The carbon film remains sticked to the mica instead of
} floating on the water surface. Is there any trick to overcome this
} trouble ?

Dear Daniel,
The time I had problems with removing C films from mica, the humidity
was higher than usual.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



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From: walck-at-southbaytech.com
Date: Fri, 7 Oct 2005 11:41:10 -0500
Subject: [Microscopy] advice on stereomicroscopes & cameras?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I purchased several stereomicroscopes in my career. It was always
relatively easy to ask a local representative of the major brands to
bring a unit in for a day or in some cases, a week to try it out with
doing the things that you do with it. It was surprising to me how
different each brand was from one to another in how it felt using it and
how comfortable I was the different units. My suggestion is to make
some calls and book some appointments with your local reps and see what
the consensus is among the different users in the lab after they have
had some hands on with each unit.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: Louise_Harner-at-albint.com [mailto:Louise_Harner-at-albint.com]
Sent: Friday, October 07, 2005 6:19 AM
To: Walck-at-SouthBayTech.com


I've been asked by two co-workers to provide purchasing advice for
stereomicroscopes with digital image capture and continuous zoom of
about 1 to 6 - ideally with options to increase/decrease magnification
ranges.

One co-worker will be looking at opaque samples - usually rough cut to a
few inches thick and sometimes further polished. The structures he's
examining are varied shades of gray and black, thus lighting options,
control, & reproducibility are important.

The other person will be looking at machined metal parts similar to
long, thick needles with barbs on them. He's interested in measuring
angles and other dimensions as well as seeing imperfections on the
parts.

I have trinocular Olympus stereomicroscopes with a variety of lighting
and lens options, and I'm happy with them for my research lab use. I've
also checked out other companies' products via the web. But I'd like
some real-world feedback on other stereomicroscopes - any you are
thrilled with? any you would never buy again? any really good values for
the money?


What are the current favorites for digital image capture - consumer,
prosumer, & professional level digital cameras? I prefer at least 3
megapixels (more is always acceptable since the images can be
downsized). Any comments on ease of use, quality, value, & satisfaction
from recent purchasers?


Please respond directly to me at "Louise_Harner-at-albint.com" so we don't
tie up the listserver with personal opinions. Vendor replies welcome,
but please give pricing info. I need to get back to my co-workers by
Oct. 14.

Thanks in advance!

- Louise

Louise Harner
Research Microscopist, Albany International Research Co.
777 West Street, P.O. Box 9114, Mansfield, MA 02048
phone: 508-337-9529
Louise_Harner-at-albint.com


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From: larry-at-cymru.freewire.co.uk
Date: Fri, 7 Oct 2005 11:45:23 -0500
Subject: [Microscopy] Re: AskAMicroscopist:TEM of porous silicon samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Have you actually tried any standard methods yet? And if so, what
were the problems? What is the initial form of your samples? How are
the pores created and what size are they?? Are the samples amorphous,
polycrystalline or single crystal? If single crystal, what
orientation?

I don't have personal experience of this material but methods I would
try include:

1. Mechanical pre-preparation - diamond saw, ultrasonic disc cutter,
grinding and polishing to get 3 mm discs, ~500 um thick, dimple
grinder then ion thin.

2. If the ion thinning destroys the pore structure, then it may be
possible to mechnically polish with a dimple grinder to electron
transparency. Si becomes transparent to light when very thin. This
can be used to control the final dimple polishing.

3. Easiest method, if pores are small, might be to break and grind
the Si until very fine, disperse in ethanol and dry down onto a C
support film on a grid.

--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)

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From: W.Muss-at-salk.at
Date: Fri, 7 Oct 2005 11:54:33 -0500
Subject: [Microscopy] AW: paraffin sections embedding into plastic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good afternoon,
dear Tea,

unfortunately at the moment I can't find the respective file dealing with
the } POP-OFF {-technique I used for long time earlier(1980-1990ies), so I
would like to inform you of a website-document you can read after for this
"technique".

You can find this paper at
http://www.ebsciences.com/papers/large_block.htm (scrolling down to the
section "POP-OFF-Technique").
For your convenience I have pasted in here the text:

citation } A size 3 BEEM capsule is filled with liquid resin until it is
slightly concave. The capsule is inverted quickly, placed over the
etched/marked area, and polymerized according to the plastic used. Lowicryl
sections pop off best with a resin mixture using half the amount of BEE.
Polymerized capsules are popped off the glass slides by dipping them
quickly in and out of a dewar containing liquid nitrogen (they must not be
frozen too long). Capsules will be easily lifted off. The pop-off capsules
are stained in 1.0% methylene blue at room temperature for 30 to 60
seconds, rinsed in distilled water, and dried. The capsules are examined
under a light microscope (condenser down) to determine the flatness of the
etched tissue section embedded in the pop-off's surface. If the etched area
is uniformly stained and appears in one plane of focus, it is suitable for
thin sectioning. { {End of quote

The separation of (WELL/THOROUGHLY impregnated [former] paraffin sections
reeembedded into and then polymerized resin [IMO it doesn't matter if epoxy
or acrylic one's] everytimes in my experience was tricky and not every
section/slide behaved in a same/similar manner concerning separation
(tendencies).....

Usually, you will have to mount an empty resin block over the section at
the location desired (I never heard or read about separating a whole
section from the glass-slide without decreasing area and mounting - if you
like - one or more blank resin blocks on the slide).
After polymerisation of the blocks in position over the chosen location you
better scratch the edges around the block-section contact with a sharp
scalpel.

The "trick" is - IMO - NOT to plunge the whole slide INTO liquid nitrogen,
BUT -instead - only to place the slide e.g. 1 mm ABOVE the LN2, and finally
(after 1-2 seconds of having done so to reduce thermal capacity of the
glass slide) to get the slide with its lower surface ON to the LN2 surface.

You will see "freezing" of the slide (last time point for you: when the
thicker edges of the mounted resin blocks get white !):
THEN:
either you will "hear" a "pop" (this } popping { gave the name to the
technique) or not: if the latter occurs, try to push the block by your
fingers in a +/- horizontal direction. Sometimes it is necessary to try
that procedure several times.

If you don't have a positive result (a likely result for silane coated
slides), try - as a last trial for rescue - the following:
before performing the pop-off technique on LN2 surface, place the slide
with its resin blocks mounted into the oven at -say- 90 degrees C for 15
min or so, then transfer as quickly as possible to the cold LN2 surface....

Physical Mechanism (as to my knowledge): it is clear that the different
thermal capacity of the glass slide and the resin blocks/section on the
other hand will result in different expansion properties (extension
modulus) if cooled down to - 196 degrees C. Therefore it is necessary to
get a very cold glass slide, but instead a more/less "warm" resin block
boundary of the reembedded section.


Hope this helps,
Best wishes and regards

Wolfgang Muss

Personal Communication Data:
OR Dr. Wolfgang Muss Member of
EM-Lab
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively

Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o
W.Muss")
E-Mail work: W.Muss-at-SALK.at

Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at
------------------------------------------------------------------------
--------------------------------------
Ankuendigung namens der (Information on behalf of)
Society for Cutaneous Ultrastructure Research (SCUR)
PLEASE VISIT THE UPDATED WEBSITE of SCUR at
} http://www.scur.org {
-------------------------------------------------------------------------
Forthcoming Meetings:
33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland
Preliminary informations: send an E-Mail
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Czech Republic

35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan
Joint Meeting with the
JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology


----------
Von: meulia.1-at-osu.edu[SMTP:meulia.1-at-osu.edu]
Antwort an: meulia.1-at-osu.edu
Gesendet: Freitag, 07. Oktober 2005 18:08
An: W.Muss-at-salk.at
Betreff: [Microscopy] paraffin sections embedding into plastic




------------------------------------------------------------------------
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The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

We have some paraffin sections on glass slides (silane coated, ems
slides) that we would need to embed into Spurr's resin.

Does anyone have a suggestion on how to separate the glass from the
Spurr's resin blocks? We briefly plunged them into liquid nitrogen,
but we did not get consistent results.

Also, shall we use a different resin?

Thank you.

Tea Meulia


--
***************************************
Tea Meulia
Molecular and Cellular Imaging Center
Ohio State University/OARDC
1680 Madison Ave.
Wooster OH 44691

tel.: 330-263-3836 or -3828
fax: 330-202-3563

http://www.oardc.ohio-state.edu/mcic

*****************************************

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From: lcgould-at-med.cornell.edu
Date: Fri, 7 Oct 2005 12:06:47 -0500
Subject: [Microscopy] Re: paraffin sections embedding into plastic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tea,
Separating sections from plain glass slides, or even the old
fashioned albumin or gelatin coated slides usually worked pretty well
using the liq. nitrogen method. Silanized slides (or "plus" slides)
seem to be a whole 'nother animal. The treatment is designed to keep
the sections on the slide...and it does.
I suppose you could try etching away the glass with HF...but that is
very dangerous and not a step to take lightly. If you do go that
route, I would use a diamond scribe to etch and break the slide as
close to the block face at possible in order to minimize both the
amount of HF needed, and the time to dissolve the glass.
If you think you need to use HF, contact me off-list and I will
regale you with precautions to take.
Lee



--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175

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From: walck-at-southbaytech.com
Date: Fri, 7 Oct 2005 12:54:54 -0500
Subject: [Microscopy] Re: AskAMicroscopist:TEM of porous silicon samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would definitely add the small angle cleavage technique to the
standard technique (aka MicroCleave(TM) technique) for this. If you
need information on this technique, please visit our website and look up
application notes associated with the Model 520.

I've seen cross sections of porous silicon samples in the literature and
it didn't appear that they had done anything different. The epoxies
that are used should infiltrate the porous silicon fairly well. You
should be able to use ay of the standard techniques for cross section
sample prep. A number of application notes are available on our web
site that describe these techniques.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com

Disclaimer: South Bay Technology manufactures and sells the
MicroCleave(TM) and the full complement of TEM sample preparation
equipment.


-----Original Message-----
X-from: larry-at-cymru.freewire.co.uk [mailto:larry-at-cymru.freewire.co.uk]
Sent: Friday, October 07, 2005 9:49 AM
To: Walck-at-SouthBayTech.com

} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America

Have you actually tried any standard methods yet? And if so, what
were the problems? What is the initial form of your samples? How are
the pores created and what size are they?? Are the samples amorphous,
polycrystalline or single crystal? If single crystal, what
orientation?

I don't have personal experience of this material but methods I would
try include:

1. Mechanical pre-preparation - diamond saw, ultrasonic disc cutter,
grinding and polishing to get 3 mm discs, ~500 um thick, dimple
grinder then ion thin.

2. If the ion thinning destroys the pore structure, then it may be
possible to mechnically polish with a dimple grinder to electron
transparency. Si becomes transparent to light when very thin. This
can be used to control the final dimple polishing.

3. Easiest method, if pores are small, might be to break and grind
the Si until very fine, disperse in ethanol and dry down onto a C
support film on a grid.

--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail:
larrys-at-jeoleuro.com

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted. 2. Any
mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)

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From: eschumacher-at-mccrone.com
Date: Fri, 7 Oct 2005 13:05:25 -0500
Subject: [Microscopy] Student Poster Competition - MMMS Meeting March 24, 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

STUDENT POSTER COMPETITION ANNOUNCEMENT

To be held in conjunction with the March 24, 2006 meeting of the Midwest

Microscopy and Microanalysis Society

Affiliate of the Microscopy Society of America and the Microbeam
Analysis
Society of America

ABSTRACT DEADLINE: Abstracts must be received in electronic format by
5PM,
Friday, December 16, 2005.

The first quarter meeting of the Midwest Microscopy and Microanalysis
Society will be held on Friday, March 24, 2006, in the Pancoe Life
Sciences
Building, Northwestern University, Evanston, Illinois. A student poster

competition open to undergraduate and graduate students will be held in
conjunction with the meeting. Posters should illustrate utilization of
microscopy for either biological or materials science study. Prizes
will be
awarded as follows:

$300 for 1st place
$200 for 2nd place
$100 for 3rd place

Abstracts must be received in electronic format by 5PM on Friday,
December 16, 2005. To be eligible for a prize, you must be first author
on
the poster, and you must be present at the meeting. You are encouraged
to
submit your entry as early as possible, as space may be limited.
Abstracts
from last year's competition, and an example of the judging worksheet
can be
found on the MMMS website:


http://northwestern.edu/bioimaging/MMMS%20Website/index.htm.

Further details will be provided upon acceptance of your submission.

Please send the following information to the email address below, and
attach
your abstract as a Word document:

Name Phone number
Affiliation Email address
Mailing address

Send to:

Elaine Schumacher
MMMS Materials Science Director
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539
Tel: 630-887-7100 Fax: 630-887-7417
Email: eschumacher-at-mccrone.com


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From: geil-at-uiuc.edu
Date: Fri, 7 Oct 2005 15:50:06 -0500
Subject: [Microscopy] TEM, replica removal

Contents Retrieved from Microscopy Listserver Archives
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Dear Daniel:
We routinely remove Pt/C replicas or shadowed samples of
polymers, from mica or glass cover slips, after also coating with
vertically coated C, by floating on dilute (ca 1%) HF, taking all the
necessary HF use precautions. The sample can be picked up on the
grid, and either touched to a paper tissue or refloated on water to
remove any residual HF.
If any questions let me know.
Regards,
Phil Geil
--


Phillip H. Geil; Ph. 217-333-0149 Fax 217-333-2736
Department of Materials Science and Engineering
University of Illinois
1304 W. Green St.
Urbana, IL 61801

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From: abowling-at-mail.utexas.edu
Date: Sat, 8 Oct 2005 00:08:13 -0500
Subject: [Microscopy] Re: TEM: recovering replicas from mica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If you have used an electrostatic glue (i.e. poly-L-lysine) the replica
will NOT separate from the mica without using HF (in my experience). If
you do use HF to remove the replica, I would recommend using a glass rod
(yes, glass) to remove the replica to distilled water before picking up
on a grid. You just touch the rod to the surface of the liquid and
"roll" the replica up onto the rod and "unroll" it onto the surface of
the water. I feel this minimizes the stress to the replica. This
method was mentioned in a paper by John Heuser but unfortunately I don't
have the citation for you. (I made a platinum/carbon replica on a
poly-L-lysine-coated glass coverslip and removed the replica onto
full-strength HF just a few hours ago, so I know that works!)

On an HF-free note, in the book "Negative Staining and Cryoelectron
Microscopy", Robin Harris recommends letting carbon films evaporated
onto mica sit overnight before attempting to float them off onto water.
As a possible means to avoid waiting overnight, Harris suggests placing
the mica into a petri dish on some damp filter papers for a few hours.
Perhaps this might be enough to get your replicas to float.

Good luck!

Andrew Bowling
post-PhD but pre-Postdoc
The University of Texas
Austin, Texas



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From: klk-at-biotech.ufl.edu
Date: Mon, 10 Oct 2005 15:01:27 -0500
Subject: [Microscopy] Doul Layer DVD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Masood,

Generally micro-organisms aren't discovered using the electron microsope.
They are normally taken from their natural habitat, such as soil, the air,
blood and so forth, and may be grown ('cultured') in special media that
contains the nutrients they need to survive. The media may be an Agar plate
(a jelly or jello like material ) or a soupy broth liquid. The single
celled organisms divide in this producing millions of cells (and hopefully
all of the type you want). Viruses are more complicated as they require a
cell to replicate in. Once there are plenty of micro-organisms in the
culture they can be harvested and treated for viewing under the light or
electron microscope. Light microscopy is, with the use of special cellular
stains, is still very important for micro-organism identification.
Once isolated and cultured we can also study them to see how they live
and how to kill them if they are dangerous.

So normally the micro-organisms would be discovered by these traditional
collection, isolation and culturing techniques. Naturally we know most about
micro-organisms that are medically important, i.e. those that cause disease,
allergic reactions or are beneficial to us.

Have a browse through the light blue section of this (UK) link:
http://www.biotopics.co.uk/conten.html#ecology

Some pretty scanning (surface) electron microscope images are here:
http://www.pbrc.hawaii.edu/bemf/microangela/

When using electron microscopes, sometimes micro-organisms may be discovered
when viewing other material, such a plant & animal tissues or cells, e.g.
http://www.sunderland.ac.uk/~es0man/tem1.htm

In many cases new micro-organisms are 'discovered' after a new infectious
disease is identified or during research into a well known disease or
investigating things like soil fertility or the safety of drinking water or
the transfer of disease via the air. However scientists are also interested
in how the micro-organisms live rather just their appearance as seem under
electron microscope. You can use special stains (often heavy metals) under
the transmission electron microscope to highlight intra-cellular cell
structures.

A 'newly' discovered micro-organism is:
http://www.eurekalert.org/pub_releases/1996-09/ORNL-NDBP-170996.php

Regards

Keith
----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk






[----- Original Message -----

X-from: {distall2-at-yahoo.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Thursday, October 06, 2005 12:47 AM

Hello All,

What is a doul layer dvd?

And no, I don't believe that is "dual" misspelled.

--
Karen L. Kelley
ICBR Electron Microscopy Manager
University of Florida
ICBR Electron Microscopy Core Lab
Bartram Hall Room 214
Box 118525 Gainesville Florida
Lab: 352-392-1184 fax: 352-846-0251
Email: klk-at-biotech.ufl.edu
Southeastern Microscopy Society Treasurer
http://www.biotech.ufl.edu/EM/



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From: grb-at-ufl.edu
Date: Mon, 10 Oct 2005 15:28:00 -0500
Subject: [Microscopy] Re: Doul Layer DVD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I bet you a beer, payable at the New Zealand Microscopy conference early in
2007, that it is, in fact, either a typo or a manifestation of illiteracy.

cheers

rtch

Date sent: Mon, 10 Oct 2005 15:03:13 -0500
To: r.sims-at-auckland.ac.nz
X-from: klk-at-biotech.ufl.edu
Send reply to: klk-at-biotech.ufl.edu

That is a DVD that hold 'too' times the information of a standard dvd.

klk-at-biotech.ufl.edu wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
Gerald Bourne
Major Analytical Instrumentation Center
Department of Materials Science and Engineering
University of Florida
107H MAEC
P.O. Box 116400
Gainesville, FL 32611
(352) 392-6985
(352) 392-0390 fax


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From: gvrdolja-at-nature.berkeley.edu
Date: Mon, 10 Oct 2005 15:45:21 -0500
Subject: [Microscopy] oil filters

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I need to order some oil filters and odor element for two Edwards rotary
pumps (RV8). The elements go into a housing known as an EMF10. Does
anyone have an inexpensive source for these? I've seen a price of $220
for one oil filter and odor filter. Any advice appreciated.

Thanks

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
AOL/IM rakhasha http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

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From: Bede.Willenbring-at-hbfuller.com
Date: Mon, 10 Oct 2005 17:12:40 -0500
Subject: [Microscopy] Re: Doul Layer DVD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Actually, I believe it is a misspelling. If it is, it's the latest flavor of optical recording media (but not the last). They actually record on two separate layers (hence dual) on the same side of the disc.

If you dying to know the details, everything you never wanted to know about CDs' and DVD's can be had at

http://www.clir.org/pubs/reports/pub121/contents.html

The document itself is an evaluation of the life expectancy of optical media. But it contains a very understandable description of the various types of optical disk.



Bede Willenbring
Research Chemist
H.B. Fuller Company

E-mail: Bede.Willenbring-at-HBFuller.com
Phone: 651-236-5470
Fax: 651-236-5020
Correspondence: P.O. Box 64683, St. Paul, MN 55164-0683

} } } {klk-at-biotech.ufl.edu} 10/10/05 3:04 PM } } }



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Hello All,

What is a doul layer dvd?

And no, I don't believe that is "dual" misspelled.

--
Karen L. Kelley
ICBR Electron Microscopy Manager
University of Florida
ICBR Electron Microscopy Core Lab
Bartram Hall Room 214
Box 118525 Gainesville Florida
Lab: 352-392-1184 fax: 352-846-0251
Email: klk-at-biotech.ufl.edu
Southeastern Microscopy Society Treasurer
http://www.biotech.ufl.edu/EM/



==============================Original Headers==============================
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From: paul_hazelton-at-umanitoba.ca
Date: Mon, 10 Oct 2005 21:57:29 -0500
Subject: [Microscopy] thanks re Van Leeuwenhoek lenses and microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

to all who responded to my plaintif plea, thanks. you were all correct. unfortunately, the company no longer carries their line of historical objects. but i did get some interesting information. seems the microscopes were a labour of love by an "old doctor in Chicago" who custom made the replicas. they were full size, working models in steriling silver. the company purchased 4 to see if they would be a seller, but no-one took them up on them. eventually they were sold off. the person who made the instruments is apparently "no longer with us, he's gone on...." in the words of the source.

suppose i should give recognition to Carolina Biological and their 30year employee/microscope expert who passed the information on.

oh, and phil - the other companies were a good thought, but they couldn't help. one did suggest i contact an antique dealer. i don't even want to think what an original would cost. not even if i won your Power Ball lottery that you guys run in some of the states.....

paul

Paul R. Hazelton, PhD
Electron Microscope Unit
University of Manitoba
Department of Medical Microbiology
531 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 0W3
e-mail: paul_hazelton-at-umanitoba.ca
Phone:204-789-3313
Pager:204-931-9354
Cell:204-781-1502
Fax:204-789-3926

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From: tylko-at-zuk.iz.uj.edu.pl
Date: Tue, 11 Oct 2005 02:14:58 -0500
Subject: [Microscopy] thanks re vacuum pumps and LaB6

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to thank everyone who replied to my questions concerning vacuum
and electron gun modification.
Regards,

Dr Grzegorz Tylko
Department of Cytology and Histology
Institute of Zoology
Jagiellonian University
ul. Ingardena 6
30-060 Krakow
fax: +48-12-634-49-51
phone: +48-12-633-63-77 ext. 2425
mobile phone: +48-602-535-041
e-mail: tylko-at-zuk.iz.uj.edu.pl


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From: David.Patton-at-uwe.ac.uk
Date: Tue, 11 Oct 2005 02:48:32 -0500
Subject: [Microscopy] Re: Doul Layer DVD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Can I join in the fun? Bon Jovi are releasing a Dual disc soon.
Apparently it is a CD one side and a DVD on the other. Yippee - I would
also like my computer to play 7" singles.

Dave

-----Original Message-----
X-from: Bede.Willenbring-at-hbfuller.com
[mailto:Bede.Willenbring-at-hbfuller.com]
Sent: 10 October 2005 23:20
To: David Patton

Actually, I believe it is a misspelling. If it is, it's the latest
flavor of optical recording media (but not the last). They actually
record on two separate layers (hence dual) on the same side of the disc.

If you dying to know the details, everything you never wanted to know
about CDs' and DVD's can be had at

http://www.clir.org/pubs/reports/pub121/contents.html

The document itself is an evaluation of the life expectancy of optical
media. But it contains a very understandable description of the various
types of optical disk.



Bede Willenbring
Research Chemist
H.B. Fuller Company

E-mail: Bede.Willenbring-at-HBFuller.com
Phone: 651-236-5470
Fax: 651-236-5020
Correspondence: P.O. Box 64683, St. Paul, MN 55164-0683

} } } {klk-at-biotech.ufl.edu} 10/10/05 3:04 PM } } }



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The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America

Hello All,

What is a doul layer dvd?

And no, I don't believe that is "dual" misspelled.

--
Karen L. Kelley
ICBR Electron Microscopy Manager
University of Florida
ICBR Electron Microscopy Core Lab
Bartram Hall Room 214
Box 118525 Gainesville Florida
Lab: 352-392-1184 fax: 352-846-0251
Email: klk-at-biotech.ufl.edu
Southeastern Microscopy Society Treasurer
http://www.biotech.ufl.edu/EM/



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From: christophe.leterrier-at-espci.fr
Date: Tue, 11 Oct 2005 03:09:43 -0500
Subject: [Microscopy] Re: Doul Layer DVD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Guess it's a "dull" layer DVD... Seems kinda crappy

Christophe

Le 10 oct. 05, à 22:05, klk-at-biotech.ufl.edu a écrit :

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} Hello All,
}
} What is a doul layer dvd?
}
} And no, I don't believe that is "dual" misspelled.
}
} --
} Karen L. Kelley
} ICBR Electron Microscopy Manager
} University of Florida
} ICBR Electron Microscopy Core Lab
} Bartram Hall Room 214
} Box 118525 Gainesville Florida
} Lab: 352-392-1184 fax: 352-846-0251
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} Southeastern Microscopy Society Treasurer
} http://www.biotech.ufl.edu/EM/
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From: W.Muss-at-salk.at
Date: Tue, 11 Oct 2005 03:16:28 -0500
Subject: [Microscopy] Re: Doul Layer DVD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello and good morning all,

Just only } supplementary {, what I found:


http://www.dvd12.info//dvd-13.html
scroll down, left link-bar-area, Press (Control&letterF) (FIND-Function
,search for } doul {) you will find the term (& LINK bar) "Doul Layer DVD"
but no further explanation exept "Double Layer DVD"

Also on
http://nessmp3.com/forums/index.php?showtopic=579
with the } find function { (Ctrl&letter F) and searching for } doul {:

you will find:

..........} Doul Layer DVD's { right now in my opinion are just a waste of
money. You need a player that supports the format and they cost big $$$.
I'de wait a few years before that investment. I know most burners support
it right now but do you want to shorten the life of your burner to watch a
movie anyway? {

Seems to be an insiders' "short cut" for Double layer DVD ?...Thanks for
the question, I haven't heared anything about such new recording medium
until now...interesting....

regards,
Wolfgang Muss
SALK (Salzburger Landeskliniken GesmbH)
Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o
W.Muss")
E-Mail work: W.Muss-at-SALK.at
Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at



----------
Von: David.Patton-at-uwe.ac.uk[SMTP:David.Patton-at-uwe.ac.uk]
Antwort an: David.Patton-at-uwe.ac.uk
Gesendet: Dienstag, 11. Oktober 2005 09:52
An: W.Muss-at-salk.at
Betreff: [Microscopy] Doul Layer DVD




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The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Can I join in the fun? Bon Jovi are releasing a Dual disc soon.
Apparently it is a CD one side and a DVD on the other. Yippee - I would
also like my computer to play 7" singles.

Dave

-----Original Message-----
X-from: Bede.Willenbring-at-hbfuller.com
[mailto:Bede.Willenbring-at-hbfuller.com]
Sent: 10 October 2005 23:20
To: David Patton

Actually, I believe it is a misspelling. If it is, it's the latest
flavor of optical recording media (but not the last). They actually
record on two separate layers (hence dual) on the same side of the disc.

If you dying to know the details, everything you never wanted to know
about CDs' and DVD's can be had at

http://www.clir.org/pubs/reports/pub121/contents.html

The document itself is an evaluation of the life expectancy of optical
media. But it contains a very understandable description of the various
types of optical disk.



Bede Willenbring
Research Chemist
H.B. Fuller Company

E-mail: Bede.Willenbring-at-HBFuller.com
Phone: 651-236-5470
Fax: 651-236-5020
Correspondence: P.O. Box 64683, St. Paul, MN 55164-0683

} } } {klk-at-biotech.ufl.edu} 10/10/05 3:04 PM } } }



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Hello All,

What is a doul layer dvd?

And no, I don't believe that is "dual" misspelled.

--
Karen L. Kelley
ICBR Electron Microscopy Manager
University of Florida
ICBR Electron Microscopy Core Lab
Bartram Hall Room 214
Box 118525 Gainesville Florida
Lab: 352-392-1184 fax: 352-846-0251
Email: klk-at-biotech.ufl.edu
Southeastern Microscopy Society Treasurer
http://www.biotech.ufl.edu/EM/



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From: gcc-at-couger.com
Date: Tue, 11 Oct 2005 03:33:49 -0500
Subject: [Microscopy] Re: thanks re Van Leeuwenhoek lenses and microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



paul_hazelton-at-umanitoba.ca wrote:

} to all who responded to my plaintif plea, thanks. you were
all correct. unfortunately, the company no longer carries their
line of historical objects. but i did get some interesting
information. seems the microscopes were a labour of love by an
"old doctor in Chicago" who custom made the replicas. they were
full size, working models in steriling silver. the company
purchased 4 to see if they would be a seller, but no-one took
them up on them. eventually they were sold off. the person who
made the instruments is apparently "no longer with us, he's gone
on...." in the words of the source.
}
} suppose i should give recognition to Carolina Biological and
their 30year employee/microscope expert who passed the
information on.
}
} oh, and phil - the other companies were a good thought, but
they couldn't help. one did suggest i contact an antique
dealer. i don't even want to think what an original would cost.
not even if i won your Power Ball lottery that you guys run in
some of the states.....
}
Hi Paul,

Commissioning some one to build a few might not be too expensive
if you can find the right person. To be authentic they are have
to almost be made one off any way. Brass or copper would be more
authentic than silver and take on a more pleasing patina with
age. If you hurry copper will cost less than silver:{

Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org


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From: pgrover-at-bilbo.bio.purdue.edu
Date: Tue, 11 Oct 2005 09:14:41 -0500
Subject: [Microscopy] thanks re Van Leeuwenhoek microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from a quick search of the web doul layer is obviously a misspelling of dual
layer (e.g. Chitty Chitty Bang Bang on doul layer disk - I don't think so).
Dual layer where the laser can actually write to two dye layers on one side
of the disk. This doubles the capacity of the disk from 4.7Gb to 9.4Gb and
is the reason DVD film copying requires a great deal of time to compress the
9.4Gb into 4.7Gb of a -R or +R standard single layer DVD - up to 8 hours
(whereas decrypting the copyright protection takes a few minutes).

With regard to backing up important scientific data, you should be aware
that archived CD-Rs should be 're-backuped' up to new media every three to
five years. Unbranded CD's that have little quality control should be
avoided or re-backed up within 6 months of writing. Use datasafe pens (if
anything) for writing on the label surface (solvent based 'sharpies' aren't
recommended at all, especially the manufacturer), as the CD label surface is
actually where the read/write dye is located. Store crucial archived CDs or
DVD's in solid tough polythene cases, although paper CD sleeves are fine if
handled carefully. Avoid all-polythene clear flexible sleeves of the type
often sent out with free CD's as these definitely do gradually damage the CD
surface by rubbing.

Of the DVD's only DVD RAM is safe for storage up to 100 years and 300,000
re-writes (especially in its protective caddy) - although the format is
difficult to source as a drive these days in its caddy version and the lack
of a recovery drive may become a problem in the future. The 'drag&drop'
4.7Gb DVD-RAM can be removed from the caddy or purchased as plain non-caddy
disks. LG make excellent non caddy DVD-RAM/-R/+R multiwriters. DVD RAM,
being read/write erasable, are very slow to write to though.

DVD dual layer, -R & +Rs are great for film and TV archives where a scratch
may just result in the odd dropped frame and at worst a trip to the mall to
buy another copy. However that could be your precious image or data file.
DVD -R and +Rs scratch very easily, even when stacking or dropping onto
carpet, so although they are certainly far better than nothing, all
computers in our imaging suite use DVD RAM cartridge drives as well as DVD-R
and CD for crucial data. If possible keep another copy of crucial data on a
PC hard drive as well (they are so cheap per Gb these days and relatively
reliable). Internal hard drives are less likely to suffer from shock
(dropping them or things onto them), which is particularly a problem when
active with the head unparked (causing the HD head to crash onto the disk
surface when read/writing). Secondary internal hard drives are very easy to
fit - but keep to one manufacturer. Personally I would avoid dual layer
DVD's at the moment as they are really for hi-definition film, are expensive
per Gb, and their complexity may lead to data loss in the future (early CD's
have already failed due to the printing ink of the label gradually etching
into the dye layer).

Always backup with proper backup software such as Nero backItUp where the
data is verified as an exact copy of the original file. I never compress
files, and large zipped directories frequently corrupt. Personally I always
avoid any type of tape backup system, as whenever I have tried to recover
lost data the tape archive has been 'corrupted' and I have had to recover
data from a standard floppy disk or CD. I keep backup DVD's of my home data
at work and visa-versa.

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk
----- Original Message -----
X-from: {Bede.Willenbring-at-hbfuller.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Monday, October 10, 2005 11:19 PM

I like to add to add the following link for those who are interested in food and other micro-organisms under electron microscopes.
http://www.magma.ca/~scimat/


Ann Fook Yang
EM Unit/ Unite EM
AAFC/AAC
960 Carling Ave,
Ottawa,Ontario
Canada K1A 0C6
yanga-at-agr.gc.ca
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
X-from: keith.morris-at-ucl.ac.uk [mailto:keith.morris-at-ucl.ac.uk]
Sent: Monday, October 10, 2005 6:35 AM
To: Yang, Ann-Fook

Dear Masood,

Generally micro-organisms aren't discovered using the electron microsope.
They are normally taken from their natural habitat, such as soil, the air,
blood and so forth, and may be grown ('cultured') in special media that
contains the nutrients they need to survive. The media may be an Agar plate
(a jelly or jello like material ) or a soupy broth liquid. The single
celled organisms divide in this producing millions of cells (and hopefully
all of the type you want). Viruses are more complicated as they require a
cell to replicate in. Once there are plenty of micro-organisms in the
culture they can be harvested and treated for viewing under the light or
electron microscope. Light microscopy is, with the use of special cellular
stains, is still very important for micro-organism identification.
Once isolated and cultured we can also study them to see how they live
and how to kill them if they are dangerous.

So normally the micro-organisms would be discovered by these traditional
collection, isolation and culturing techniques. Naturally we know most about
micro-organisms that are medically important, i.e. those that cause disease,
allergic reactions or are beneficial to us.

Have a browse through the light blue section of this (UK) link:
http://www.biotopics.co.uk/conten.html#ecology

Some pretty scanning (surface) electron microscope images are here:
http://www.pbrc.hawaii.edu/bemf/microangela/

When using electron microscopes, sometimes micro-organisms may be discovered
when viewing other material, such a plant & animal tissues or cells, e.g.
http://www.sunderland.ac.uk/~es0man/tem1.htm

In many cases new micro-organisms are 'discovered' after a new infectious
disease is identified or during research into a well known disease or
investigating things like soil fertility or the safety of drinking water or
the transfer of disease via the air. However scientists are also interested
in how the micro-organisms live rather just their appearance as seem under
electron microscope. You can use special stains (often heavy metals) under
the transmission electron microscope to highlight intra-cellular cell
structures.

A 'newly' discovered micro-organism is:
http://www.eurekalert.org/pub_releases/1996-09/ORNL-NDBP-170996.php

Regards

Keith
----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk






[----- Original Message -----

X-from: {distall2-at-yahoo.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Thursday, October 06, 2005 12:47 AM

Paul,
Alan Shinn used to make replicas and sell them, I think for $ 100, but I
think he no longer does. If you can't find some place to buy one, or can't
convince your coworkers to buy you one, you could always make one yourself,
as you'll need something to do in retirement ;o) You can see his plans
here:

http://www.mindspring.com/~alshinn/Leeuwenhoekplans.html

Be sure to check out Roger Baker's great article for a not-so-authentic, but
more functional design, as well as tons more info. on lens making:

http://www.science-info.org/pages/Roger%20Baker/homemade-microscope.pdf


I'm putting the finishing touches on my own Leeuwenhoek replica, so keep in
touch and maybe I'll be able to help you out.

Paul Grover



------------------------------------------------------------------------
May your trails be crooked, winding, lonesome, dangerous, leading to the
most amazing view.

- Edward Abbey




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From: wesaia-at-iastate.edu
Date: Tue, 11 Oct 2005 09:17:15 -0500
Subject: [Microscopy] Re: Re: Doul Layer DVD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I appreciate Wolfgang's digging to see if it might be something real.
However, checking out those pages and doing my own search for "doul DVD"
and "dual DVD", I suspect it is just a spelling error. There were several
other spelling errors on those pages which drops their credibility as an
authoritative source. Maybe doul will catch on as a tricky abbreviation for
double that plays on the word dual. For now, I write it off as a mispelling.

Warren

At 03:17 AM 10/11/05, you wrote:

} Hello and good morning all,
}
} Just only } supplementary {, what I found:
}
}
} http://www.dvd12.info//dvd-13.html
} scroll down, left link-bar-area, Press (Control&letterF) (FIND-Function
} ,search for } doul {) you will find the term (& LINK bar) "Doul Layer DVD"
} but no further explanation exept "Double Layer DVD"
}
} Also on
} http://nessmp3.com/forums/index.php?showtopic=579
} with the } find function { (Ctrl&letter F) and searching for } doul {:
}
} you will find:
}
} ..........} Doul Layer DVD's { right now in my opinion are just a waste of
} money. You need a player that supports the format and they cost big $$$.
} I'de wait a few years before that investment. I know most burners support
} it right now but do you want to shorten the life of your burner to watch a
} movie anyway? {
}
} Seems to be an insiders' "short cut" for Double layer DVD ?...Thanks for
} the question, I haven't heared anything about such new recording medium
} until now...interesting....
}
} regards,
} Wolfgang Muss
} SALK (Salzburger Landeskliniken GesmbH)
} Paracelsus Medical Private University (PMU)
} Institute of Pathology
} Electron Microscopy Lab
} Muellner Hauptstrasse 48
} A-5020 SALZBURG, Austria/Europe


-------------------------------------------
No files should be attached to this message
-------------------------------------------
Warren E. Straszheim, Ph.D.
Materials Analysis and Research Lab
Iowa State University
46 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking


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From: tisdale-at-vision.eri.harvard.edu
Date: Tue, 11 Oct 2005 10:31:38 -0500
Subject: [Microscopy] SEM height measurements

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to measure height differences between microvilli on
cultured cells that have been imaged by SEM. Is there a program
available that does this type of measurement? Would Image J work? Is
there a direct correlation between brightness of structure and
height of surface structure that could be used in this manner to
compare height of microvilli on adjacent cells? Any opinions would
be welcomed.

Thanks,

Ann Tisdale
Research Associate
Schepens eye Research Institute
Boston, MA 02114
email: tisdale-at-vision.eri.harvard.edu


==============================Original Headers==============================
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From: Mike.Bode-at-soft-imaging.net
Date: Tue, 11 Oct 2005 11:50:06 -0500
Subject: [Microscopy] SEM height measurements

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Ann,

Local topography is only one factor that influences the signal you get from a sample. Others are variations in scattering coefficients (mainly the atomic number of the sample), edges, orientation to detector, etc. In other words, there is no direct correlation between brightness and structure, unless you have a very simple case. I doubt that you can get reliable topographic information from just a single image. I think, your best try would be to model a structure and compare the results of a simulation with real measurements. You can google "Monte Carlo Electron" and find references to electron scattering simulations. David Joy has been working in this field for some time.

If you can take stereo images (two images at different angles) of your sample, you might be able to get some height information, but it appears that the height differences are very small, so that might not be a possibility either.

The only other technique that I can think of right now would be an AFM type of measurement. Have you looked into that?

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
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email:  mailto:info-at-soft-imaging.com
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===================================


-----Original Message-----
X-from: tisdale-at-vision.eri.harvard.edu [mailto:tisdale-at-vision.eri.harvard.edu]
Sent: Tuesday, October 11, 2005 9:39 AM
To: Mike Bode

I would like to measure height differences between microvilli on cultured cells that have been imaged by SEM. Is there a program available that does this type of measurement? Would Image J work? Is there a direct correlation between brightness of structure and height of surface structure that could be used in this manner to
compare height of microvilli on adjacent cells? Any opinions would
be welcomed.

Thanks,

Ann Tisdale
Research Associate
Schepens eye Research Institute
Boston, MA 02114
email: tisdale-at-vision.eri.harvard.edu


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From: allan.mitchell-at-stonebow.otago.ac.nz
Date: Tue, 11 Oct 2005 22:49:15 -0500
Subject: [Microscopy] Birefringence of Fibers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Keith

as far as I understand the DVDs may be dual layer but I think their
capacity isn't twice 4.7Gb but nearer to 8.4Gb. At typically 5x the
price (or more) of a 4.7Gb DVD this makes them appear even more
uneconomical for data storage. I assume the problem is that they are
more likely to be used to 'rip' a video DVD and this may be reflected
in the price - but I don't know.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
University of Sunderland
Tyne & Wear
SR1 3SD
UK

e-mail: malcolm.haswell-at-sunderland.ac.uk



----- Original Message -----
X-from: keith.morris-at-ucl.ac.uk

Hi all

A request from one of our Centre's users. Can anybody help out.

They are looking for the following article from The Microscope Journal
( - an international journal dedicated to the advancement of all forms
of microscopy for the biologist, mineralogist, metallographer or
chemist). Published Quarterly by McCrone Research Institute, Edited by
Dr. Gary Laughlin.

They are after the following article: Gorski, A., and McCrone, W.C.
“Birefringence of Fibers,” THE MICROSCOPE, Vol.46:1, 1998, pp. 3-16

Very happy to pay for photocopying, postage....





Allan Mitchell
Otago Centre for Electron Microscopy
Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 5086 or 479 7254

EM Centre: http://ocem.otago.ac.nz/
Confocal Centre: http://occm.otago.ac.nz/
Department: http://anatomy.otago.ac.nz/



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From: oshel1pe-at-cmich.edu
Date: Wed, 12 Oct 2005 08:00:36 -0500
Subject: [Microscopy] RE: SEM height measurements

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Malcolm,

I must admit I only use double (or is it dual*) sided DVD RAM which
conveniently are naturally exactly 2x4.7GB and so really are 9.4Gb,
but you do have to remove the cartridge and turn it over to get that
extra 4.7Gb (remember the days of the 'B' side?). Of course once its
formatted you get that Gb, bits and bytes thing where the actually
capacity is always lower than 4.7Gb anyway.

I've never used an 8.5Gb dual layer DVD at £3.00+ when 4.7Gb
DVD-R's are far cheaper at 9p and are the only format used by
my Panasonic DVD-RAM multidrives at work (and Toshiba DVD
RAM video recorder and PC's at home). I am adding dual layer DVD
multiwriters (replacing the CD RW's) to co-exist with all my DVD-RAM
cartridge drives as these can write standard DVD -R's at 16x and CD's at 30x
or more (and to dual layer if required in the future). Optical drives are so
incredibly cheap at the moment (and being so are definitely less reliable -
so I always verify each burn).

http://www.dual-layer-dvd.co.uk/

Regards
Keith

*But probably not doul

----- Original Message -----
X-from: {malcolm.haswell-at-sunderland.ac.uk}
To: {keith.morris-at-ucl.ac.uk}
Sent: Tuesday, October 11, 2005 5:57 PM

Ann,

There are programs that will do what you want. Metaxa is one (although expensive), and there very likely is an ImageJ plug-in. The more important question is: do you really want to do these measurements, or can they be done?
There are several problems with measuring biological structures in the SEM:
1) Such measurements require stereopairs and appropriate trignonometry. These methods are well known by the photogrammetry people and are in the literature.
2) Fixation, dehydration, and drying all cause uncontrolled dimensional changes in structures. Further, as the samples sit in storage, they continue to shrink for some unknown time. And possibly can swell some amount when being moved through the air, picking up water from the atmosphere, into and out of the SEM chamber.
2a) These changes are dependent on the mechanical nature of the sample, and its physicochemical properties. This means that the dimensional changes will vary along the different axes as the sample makeup varies. Fibers for instance will behave differently along their length than they will across their width. This also applies to ultrastructure, since again the physicochemical properties are not identical in all directions. Plus, of course, the samples must have been handled *exactly* identically at all stages.
So any differences in microvilli height are much more likely due to specimen processing and handling than to any intrinsic difference between e.g., cells.
The possible exceptions are structures that are mechanically rigid enough to not be affected by the processing. Well-tanned insect cuticle and wood may fit this requirement. Calcified bone and teeth do. Cells don't.
The only possible way around this particular problem is to do the work on properly cryofixed cells in a cryoSEM, and therefore not do any fixation, dehydration, etc. This means that unless the cells can be kept frozen in storage and never warmed much getting them into and out of the cryoSEM, the samples are only good when first examined. No going back to a sample.
3) I routinely see major differences in microvilli, such as presence/absence, and other surface ultrastructure on neighboring (as in right next to each other) cells. So, the cells were handled "*exactly* identically at all stages". But what does this mean? Most likely, just that the cells were at different metabolic stages. Like when looking at a section of epithelium with mucus-secreting cells. Different cells are at different stages of the secretory cycle, but it doesn't mean anything else.
4) The measurements are questionable anyway, unless the SEM is an expensive metrology instrument that has been carefully calibrated at all magnifications, working distances, tilts, etc. that are actually used. A non-metrology SEM is only good to +/- 5% accuracy at best -- if calibrated as above -- and more likely +/- 10%. Such measurements also depend on the local properties of the sample, which is highly affected by topography. The accuracy can be as low as +/- 20%. So relative statements, like "cell A's microvilli are about 120 nm +/- about X nm and cell B's microvilli are about 80 nm +/- about Y nm, so cell B's microvilli are approximately 2/3 the size of cell A' microvilli" are valid. A statement like "cell A's microvilli are 121.5 nm +/- 2 nm and cell B's microvilli are 79.3 nm +/- 3 nm" are invalid, even if measurements are made that precisely in stereopairs. The physics of imaging in the SEM isn't there, nor are the possible sources of error well enough known to allow such precision. Maybe in semiconductors or metals, but not in biological samples.
5) Feature brightness is correlated with height, but it is also correlated with many other properties of the sample. Local atomic and molecular composition, local density, local topography, etc., "local" meaning with the beam/specimen interaction volume. So, no, there is no direct, useful correlation between height and feature brightness. Although I'd be willing to bet that a particular sample (or type of sample) can be found where there is such a direct correlation, it won't be cellular ultrastructure.

Mike Bode's suggestion of something like a AFM is much more likely to give you the information you need. If the probe can be kept from ripping up the cells ...

Phil


I would like to measure height differences between microvilli on
cultured cells that have been imaged by SEM. Is there a program
available that does this type of measurement? Would Image J work? Is
there a direct correlation between brightness of structure and
height of surface structure that could be used in this manner to
compare height of microvilli on adjacent cells? Any opinions would
be welcomed.

Thanks,

Ann Tisdale
Research Associate
Schepens eye Research Institute
Boston, MA 02114
email: tisdale-at-vision.eri.harvard.edu


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From: eschumacher-at-mccrone.com
Date: Wed, 12 Oct 2005 08:38:38 -0500
Subject: [Microscopy] Birefringence of Fibers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Allan,

We have the volume, and would be happy to provide a copy of the article.
We can photocopy it, or if preferred, we can scan it and send a PDF
file. That might be a good option, as the last page is a color plate.

Please let me know what format you would like, and where to send it.

Regards,

Elaine

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
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*********************************************************************

-----Original Message-----
X-from: allan.mitchell-at-stonebow.otago.ac.nz
[mailto:allan.mitchell-at-stonebow.otago.ac.nz]
Sent: Tuesday, October 11, 2005 10:51 PM
To: Elaine F. Schumacher

Hi all

A request from one of our Centre's users. Can anybody help out.

They are looking for the following article from The Microscope Journal
( - an international journal dedicated to the advancement of all forms
of microscopy for the biologist, mineralogist, metallographer or
chemist). Published Quarterly by McCrone Research Institute, Edited by
Dr. Gary Laughlin.

They are after the following article: Gorski, A., and McCrone, W.C.
"Birefringence of Fibers," THE MICROSCOPE, Vol.46:1, 1998, pp. 3-16

Very happy to pay for photocopying, postage....





Allan Mitchell
Otago Centre for Electron Microscopy
Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 5086 or 479 7254

EM Centre: http://ocem.otago.ac.nz/
Confocal Centre: http://occm.otago.ac.nz/
Department: http://anatomy.otago.ac.nz/






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From: pgan-at-ap.ansell.com
Date: Wed, 12 Oct 2005 08:47:04 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: gold/palladium or gold coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pgan-at-ap.ansell.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, October 11, 2005 at 22:05:47
---------------------------------------------------------------------------

Email: pgan-at-ap.ansell.com
Name: Phay Fang Gan

Organization: Ansell

Education: Graduate College

Location: Shah Alam, selangor, Malaysia

Question: I was once asked by a consultant whether gold/palladium or gold coating would affect the surface morphology of a specimen coated. Appreciate much all your professional advice

---------------------------------------------------------------------------

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From: eschumacher-at-mccrone.com
Date: Wed, 12 Oct 2005 09:03:08 -0500
Subject: [Microscopy] Birefringence of Fibers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fellow Listers,

As an addendum to my previous posting, if no one else has responded, and
we are asked to provide a copy, we would of course get permission from
the McCrone Research Institute to do so, or they could be contacted
directly.

Regards,

Elaine

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************

-----Original Message-----
X-from: allan.mitchell-at-stonebow.otago.ac.nz
[mailto:allan.mitchell-at-stonebow.otago.ac.nz]
Sent: Tuesday, October 11, 2005 10:51 PM
To: Elaine F. Schumacher

Hi all

A request from one of our Centre's users. Can anybody help out.

They are looking for the following article from The Microscope Journal
( - an international journal dedicated to the advancement of all forms
of microscopy for the biologist, mineralogist, metallographer or
chemist). Published Quarterly by McCrone Research Institute, Edited by
Dr. Gary Laughlin.

They are after the following article: Gorski, A., and McCrone, W.C.
"Birefringence of Fibers," THE MICROSCOPE, Vol.46:1, 1998, pp. 3-16

Very happy to pay for photocopying, postage....





Allan Mitchell
Otago Centre for Electron Microscopy
Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 5086 or 479 7254

EM Centre: http://ocem.otago.ac.nz/
Confocal Centre: http://occm.otago.ac.nz/
Department: http://anatomy.otago.ac.nz/






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From: oshel1pe-at-cmich.edu
Date: Wed, 12 Oct 2005 09:24:02 -0500
Subject: [Microscopy] RE: [Filtered] AskAMicroscopist: gold/palladium or gold coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The coating itself won't affect the surface morphology, although the coating process might. Sputter coating can heat specimens. Normally, this isn't a problem, since sputter coaters generally are designed to keep the electrons, which do most of the heating, away from the samples. But for low melting-point samples, such as bloom on chocolate, even short bursts of coating with melt the surface. As I found out from experience.
But, at high magnification, say 30,000X and above, the "surface morphology" of the sample is modified in the sense that the structure of the coating becomes visible. Pure gold produces a lumpier coat than say 60/40 gold/palladium, and since Au/Pd is cheaper than pure Au, I find it best to just use Au/Pd targets.

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859




Email: pgan-at-ap.ansell.com
Name: Phay Fang Gan

Organization: Ansell

Education: Graduate College

Location: Shah Alam, selangor, Malaysia

Question: I was once asked by a consultant whether gold/palladium or gold coating would affect the surface morphology of a specimen coated. Appreciate much all your professional advice


==============================Original Headers==============================
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From: David.Patton-at-uwe.ac.uk
Date: Wed, 12 Oct 2005 09:33:12 -0500
Subject: [Microscopy] RE: [Filtered] AskAMicroscopist: gold/palladium or

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Is there any disadvantage in moving from Au to Au/Pd targets eg vacuum
requirements or quality/quantity of coating for low magnification
applications?

Dave


-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: 12 October 2005 15:28
To: David Patton

The coating itself won't affect the surface morphology, although the
coating process might. Sputter coating can heat specimens. Normally,
this isn't a problem, since sputter coaters generally are designed to
keep the electrons, which do most of the heating, away from the samples.
But for low melting-point samples, such as bloom on chocolate, even
short bursts of coating with melt the surface. As I found out from
experience.
But, at high magnification, say 30,000X and above, the "surface
morphology" of the sample is modified in the sense that the structure of
the coating becomes visible. Pure gold produces a lumpier coat than say
60/40 gold/palladium, and since Au/Pd is cheaper than pure Au, I find it
best to just use Au/Pd targets.

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859




Email: pgan-at-ap.ansell.com
Name: Phay Fang Gan

Organization: Ansell

Education: Graduate College

Location: Shah Alam, selangor, Malaysia

Question: I was once asked by a consultant whether gold/palladium or
gold coating would affect the surface morphology of a specimen coated.
Appreciate much all your professional advice


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From: oshel1pe-at-cmich.edu
Date: Wed, 12 Oct 2005 09:42:57 -0500
Subject: [Microscopy] RE: [Filtered] AskAMicroscopist: gold/palladium or

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

No.
The Au/Pd targets work the same as pure Au targets, and I use the same coating parameters. If you use a coating thickness monitor, like a quartz-crystal instrument, you have to change the work function and mass in the programming. If a thickness monitor isn't used, then I don't find any need for changes.
The Au/Pd coating at low mags is as good as gold.

Phil

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859



-----Original Message-----
X-from: Oshel, Philip Eugene
Sent: Wed 12-Oct-05 10:38
To: David.Patton-at-uwe.ac.uk

The coating itself won't affect the surface morphology, although the
coating process might. Sputter coating can heat specimens. Normally,
this isn't a problem, since sputter coaters generally are designed to
keep the electrons, which do most of the heating, away from the samples.
But for low melting-point samples, such as bloom on chocolate, even
short bursts of coating with melt the surface. As I found out from
experience.
But, at high magnification, say 30,000X and above, the "surface
morphology" of the sample is modified in the sense that the structure of
the coating becomes visible. Pure gold produces a lumpier coat than say
60/40 gold/palladium, and since Au/Pd is cheaper than pure Au, I find it
best to just use Au/Pd targets.

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859




Email: pgan-at-ap.ansell.com
Name: Phay Fang Gan

Organization: Ansell

Education: Graduate College

Location: Shah Alam, selangor, Malaysia

Question: I was once asked by a consultant whether gold/palladium or
gold coating would affect the surface morphology of a specimen coated.
Appreciate much all your professional advice




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From: sachep-at-rockefeller.edu
Date: Thu, 13 Oct 2005 08:34:13 -0500
Subject: [Microscopy] AskAMicroscopist: What incubation media should I use to

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sachep-at-rockefeller.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, October 13, 2005 at 04:28:56
---------------------------------------------------------------------------

Email: sachep-at-rockefeller.edu
Name: Pallavi Sachdev

Organization: Rockefeller University

Education: Graduate College

Location: New York, Ny

Question: What incubation media should I use to perform live cell imaging of cells in an open chamber. ie. buffer conditions
HEPES concentration etc.
Thank you

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From: vincent.metzger-at-philips.com
Date: Thu, 13 Oct 2005 08:35:49 -0500
Subject: [Microscopy] viaWWW: boundary grain of Wfilament

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Email: vincent.metzger-at-philips.com
Name: vincent metzger

Organization: philips

Title-Subject: [Filtered] lamps filament preparation

Question: I would like to study the boundary grain of Wfilament of lamp. I'm searching a way to prepare the sample to make good observation of the grain boundary.

---------------------------------------------------------------------------

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From: curt-at-oxfordenvironment.co.uk
Date: Thu, 13 Oct 2005 08:36:14 -0500
Subject: [Microscopy] viaWWW: M7A Yellow blue edging on image

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Email: curt-at-oxfordenvironment.co.uk
Name: Curt Lamberth

Title-Subject: [Filtered] MListserver: M7A Yellow blue edging on image

Question:
Hello,

I have a Wild M7A stereomicroscope and the image(s) are edged with yellow on one side and, if you close one eye, blue on the other. This causes a yellow cast to object edges and is very annoying.

I am using either x20 or x10 eyepieces, with LED or f-optic ring illumination. The problem is most obvious under higher magnifications.

Does anyone know why this happens, and how it can be solved?

Many thanks,

Curt

---------------------------------------------------------------------------

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From: mmalecki-at-wisc.edu
Date: Thu, 13 Oct 2005 08:59:35 -0500
Subject: [Microscopy] search for the 3D reconstruction from EM tilt series software

Contents Retrieved from Microscopy Listserver Archives
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Greetings, All!
We are in the search of a 3D reconstruction from tilt series software
from EM for our undergraduate students. Would you be willing to share
your experience on various options and programs. The most basic and
generic (although addressing register alignment and missing cone),
the most desired.
Vendors welcome.


==============================Original Headers==============================
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From: flegler-at-msu.edu
Date: Thu, 13 Oct 2005 09:06:10 -0500
Subject: [Microscopy] LM Job Posting

Contents Retrieved from Microscopy Listserver Archives
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Confocal Microscopist
Center for Advanced Microscopy
Michigan State University

Full-time confocal microscopist. This is a teaching/service/support
position at the Center for Advanced Microscopy at Michigan State
University. CAM is the central microscopy laboratory for the MSU campus,
serving users from a wide variety of disciplines. The appointment will be
in the Academic Specialist category, one of the academic support ranks at
the University. The appointment will be a 12-month, annual year
appointment in the continuing appointment system. Salary will be
commensurate with experience.

Requirements include: a PhD degree in the biological sciences and a minimum
of three years experience with confocal microscopy instrumentation
including theory, operation, and maintenance. Experience in preparing and
imaging fixed and living biological tissue is required. Experience with
immunohistochemistry, Fluorescence Resonance Energy Transfer (FRET),
Fluorescence Loss in Photobleaching (FLIP) and Fluorescence Recovery After
Photobleaching (FRAP) is highly desirable as is knowledge of multi-user
facility operation. The individual must have a demonstrated competence in
and a strong commitment to graduate and post-graduate instruction and
should assist others in planning their microscopy research programs and/or
sample preparation.

The individual selected will supervise a newly funded spectral confocal
microscope, as well as two existing confocal microscopes. U.S. citizenship
is not required; applicants who are not U.S. citizens or permanent
residents must provide documentation evidencing employment authorization in
the United States. The position begins effective Spring 2006.

Submit a curriculum vita, transcripts of academic training, a statement
describing your interest in the position, evidence of teaching ability, and
arrange for three letters of recommendation to be sent to: Dr. Stanley L.
Flegler, Chair, Search Committee, Center for Advanced Microscopy, B4 CIPS
Bldg, Michigan State University, East Lansing, MI 48824,
U.S.A. Applications are due by December 1, 2005.

MSU is an Affirmative Action/Equal Opportunity Institution


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From: syli-at-northwestern.edu
Date: Thu, 13 Oct 2005 09:55:41 -0500
Subject: [Microscopy] Anew platform for microscopists to discuss microscopy online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear list,

I just created a new platform for us to discuss microscopy online using
Web Wiz Forums. It might be a better platform than listservers, especially
for those who want to reduce unnecessary emails. (I am not intending to
say this listserv is bad. In fact Nestor did a great job and the
listserv has served us for more than 10 years.) With the new online
forum you can still set email notification for the topics you are
interested in, at the same time you may ignore the topics out of
interest.

Other features of this online discussion board include:
***You need NOT register to post messages in most of the forums. However
you have to type in your name every time you post a message. Registered
users have full access to the website contents and functionality.
***Students or any Microscopists may ask questions related to microscopy
in the Discussion board.
***Employers may post vacant positions in the Jobs & Resumes.
***Job-hunters are welcome to post their resumes too.
***Users may post and discuss their recent publications in
recommended Readings.
***Open facilities or EM consulting companies may post their contact
information in EM facilities nearby.
***Software authors may post their beta tests or freewares/sharewares in
Software downloads.
***Commercial companies may post their product information and contact
information free in EM companies
***I am collecting Daily Operation Manuals for all kinds of microscopes.
Please send me your contributions to me. I will include your name as
courtesy. After I have collected sufficient amount of manuals I will put
them here so our users may benefit from your kindness.
***Finally but not least, you may create your own customized interest
group in Customized Discussion Groups. You may set it as
access-by-authorization-only or open to everyone.

The link to this forum is
http://www.ShuyouLi.com/

I welcome all kinds of comments, suggestions and criticisms - to make
us microscopists feel more convenient to discuss virtually world wide.

Thanks,
Shuyou Li


_____________________________
Shu-You Li, Ph.D.
Electron Microscopist, EPIC
NUANCE
Northwestern University
2220 Campus Drive, 1161 Cook Hall (#2036 for mail)
Evanston, IL 60208-3108, USA
Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573
Email: syli-at-northwestern.edu; shuyouli-at-gmail.com
http://www.nuance.northwestern.edu/EPIC
http://www.shuyouli.com



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10, 16 -- From: Shu-You Li {syli-at-northwestern.edu}
10, 16 -- To: microscopy-at-microscopy.com
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From: underwoo-at-u.washington.edu
Date: Thu, 13 Oct 2005 10:24:40 -0500
Subject: [Microscopy] Cryothin Immunogold?

Contents Retrieved from Microscopy Listserver Archives
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Hello All,

I have a question for all thoughs that have done immunogold labelling on
cryothin sections. I am having trouble getting consistant, predictable results.
Meaning that my signal to noise seems variable which makes it difficult to
standardize the protocol. One thing that I have noticed is a 5-15x increase in
signal (based on gold count) if I use just BSA and omit any normal serum. I
also see an increase in background but in seemingly random patterns. (Sometimes
I think the positives are much cleaner than the minus primary controls).
The protocol that I am using at present is: place freshly picked up sections on
droplet of 0.05M glycine / 1% BSA in TBS (1 hr), then 1% BSA in TBS (1 hr),
primary antibody overnight (1% BSA/TBS), secondary ( ultrasmall up to 20nm
gold) for (2 hr), fix, embed and view. My previous standard protocol was
including 5-10% normal serum in all the above solutions.
Has anyone else seen this increase in labelling frequency?
I would be interested in protocols that people are very pleased with and give
consistant clean results.
Bottom line: Have any of you found any key tricks to getting the immunogold
labelling of cryothin sections to perform without the "hitch".

Thank you for any help!

Robert Underwood
Sr. Research Scientist
U of Washington


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From: derby-at-nmt.edu
Date: Thu, 13 Oct 2005 10:46:56 -0500
Subject: [Microscopy] How to remove formalin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings to all,

I am a bit stumped.
I have been asked to find a way to remove formalin, from formalin fixed
viruses.
Any tips/trick/methods would be appreciated.

Thank you,

Robert Derby
New Mexico Tech.


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5, 16 -- Subject: How to remove formalin
5, 16 -- From: Robert {derby-at-nmt.edu}
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From: randerson20-at-tampabay.rr.com
Date: Thu, 13 Oct 2005 11:01:50 -0500
Subject: [Microscopy] Re: Anew platform for microscopists to discuss microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Very generous of you to say that you don't intend to say that the
microscopy listserver is bad.

The last thing I need is another forum sending me email. Thanks, but no
thanks. I'm sticking with Nestor where I can expect another decade or
two of continuity!

Ron Anderson

syli-at-northwestern.edu wrote:

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From: W.Muss-at-salk.at
Date: Thu, 13 Oct 2005 11:20:27 -0500
Subject: [Microscopy] AW: How to remove formalin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good afternoon,
dear Robert,
as normally virus(ses) or virus suspension have to be "inactivated" by
formaldehyde for handling, mailing and processing (at least in a
BSL2-category labs,
see, e.g., http://www.d.umn.edu/ehso/biosafety/bsl2.html and
see also specific recommendations at

http://www.rki.de/cln_011/nn_527102/SiteGlobals/Forms/Suche/en/serviceSu
cheForm,templateId=processForm.html?resourceId=231942&input_=&pageLocale
=en&searchEngineQueryString=rapid+EM&sortString=-score
or:
http://www.rki.de/cln_011/nn_231622/EN/Content/Institute/DepartmentsUnit
s/NRC/CONSULAB/download__list__04.html__nnn=true
==}
http://www.rki.de/cln_011/nn_231642/EN/Content/Institute/DepartmentsUnit
s/NRC/CONSULAB/download__list__04,templateId=raw,property=publicationFil
e.pdf/download_list_04

or see the additional separate articles on that site ),

I think that the fixation already has been 100% and finished for the
"particles" and you then are dealing only with the "remaining" fluid.

Since there is nothing left for further fixation (of virus particles,
because they ARE fixed!) then, only excess formaldehyde-containing solution
has to be removed. This can be easily done by spilling grids (with
previousely absorbed virus particles) with Aqua (tri-,bidest), in the case
you have to remove fixative from e.g. Eppendorf's (containing virus
supension in Formaldehyde solution) you are to "wash" the microtubes for
several times (after each step you have to spin down virus particles and
for such you need perhaps } an airfuge { to get the high g-forces needed).

Perhaps another possibility (I am not aware nor have tried): perhaps
filtering or using the agar-concentraion technique....
Unfortunately you have not told us the reason for you have to get rid of
the excess formaldehyde portion....

Best wishes
(and hopefully there are a lot of other interesting considerations),

regards,
Wolfgang Muss
Salzburg, Austria

OR Dr. Wolfgang Muss Member of
EM-Lab
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively

Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o
W.Muss")
E-Mail work: W.Muss-at-SALK.at

Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at

----------
Von: derby-at-nmt.edu[SMTP:derby-at-nmt.edu]
Antwort an: derby-at-nmt.edu
Gesendet: Donnerstag, 13. Oktober 2005 17:52
An: W.Muss-at-salk.at
Betreff: [Microscopy] How to remove formalin




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The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Greetings to all,

I am a bit stumped.
I have been asked to find a way to remove formalin, from formalin fixed
viruses.
Any tips/trick/methods would be appreciated.

Thank you,

Robert Derby
New Mexico Tech.


==============================Original
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5, 16 -- Subject: How to remove formalin
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25, 28 -- From W.Muss-at-salk.at Thu Oct 13 11:20:27 2005
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25, 28 -- Message-ID: {01C5D022.C4B83840.W.Muss-at-salk.at}
25, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at}
25, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at}
25, 28 -- To: "'derby-at-nmt.edu'" {derby-at-nmt.edu}
25, 28 -- Cc: "'Microscopy-at-msa.microscopy.com'" {Microscopy-at-msa.microscopy.com}
25, 28 -- Subject: AW: [Microscopy] How to remove formalin
25, 28 -- Date: Thu, 13 Oct 2005 18:20:18 +0200
25, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at}
25, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor
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From: marc.pypaert-at-yale.edu
Date: Thu, 13 Oct 2005 11:42:42 -0500
Subject: [Microscopy] Re: Cryothin Immunogold?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Robert,

We have very consistent results with a different protocol. Check it out
on our website (protocol 7):
http://cellserv.med.yale.edu/imaging/ccmi/elect_protocols.html

The main differences are:

- We use 0.1 M NH4Cl instead of glycin, but that should not matter much.
- We use 1% of fish skin gelatin instead of BSA.
- We use PBS instead of TBS, but again that should not really matter
- Incubations with antibodies and protein A-gold are for 30 min. This
could be an important factor. Why do you incubate so long with
antibodies? Maybe when antibodies don't work too well it would
make sense, but I wouldn't do this systematically. This is bound to
create problems with aggregation of antibodies, increased background,
etc. Also, we only block with NH4Cl and fish skin gelatin for a maximum
of 10 and 20 mins respectively. This means most of our immunolabelings
are completed within 2 hours! Much more cost-efficient I would say.

Good luck

Marc



On Oct 13, 2005, at 11:25 AM, underwoo-at-u.washington.edu wrote:

}
}
}
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}
} Hello All,
}
} I have a question for all thoughs that have done immunogold
} labelling on
} cryothin sections. I am having trouble getting consistant,
} predictable results.
} Meaning that my signal to noise seems variable which makes it
} difficult to
} standardize the protocol. One thing that I have noticed is a 5-15x
} increase in
} signal (based on gold count) if I use just BSA and omit any normal
} serum. I
} also see an increase in background but in seemingly random
} patterns. (Sometimes
} I think the positives are much cleaner than the minus primary
} controls).
} The protocol that I am using at present is: place freshly picked up
} sections on
} droplet of 0.05M glycine / 1% BSA in TBS (1 hr), then 1% BSA in TBS
} (1 hr),
} primary antibody overnight (1% BSA/TBS), secondary ( ultrasmall up
} to 20nm
} gold) for (2 hr), fix, embed and view. My previous standard
} protocol was
} including 5-10% normal serum in all the above solutions.
} Has anyone else seen this increase in labelling frequency?
} I would be interested in protocols that people are very pleased
} with and give
} consistant clean results.
} Bottom line: Have any of you found any key tricks to getting the
} immunogold
} labelling of cryothin sections to perform without the "hitch".
}
} Thank you for any help!
}
} Robert Underwood
} Sr. Research Scientist
} U of Washington
}
}
} ==============================Original
} Headers==============================
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} 5, 20 -- From: Robert A Underwood {underwoo-at-u.washington.edu}
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==============================Original Headers==============================
11, 20 -- From marc.pypaert-at-yale.edu Thu Oct 13 11:42:42 2005
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11, 20 -- Date: Thu, 13 Oct 2005 12:42:15 -0400
11, 20 -- From: Marc Pypaert {marc.pypaert-at-yale.edu}
11, 20 -- Subject: Re: [Microscopy] Cryothin Immunogold?
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From: W.Muss-at-salk.at
Date: Thu, 13 Oct 2005 11:45:05 -0500
Subject: [Microscopy] AW: RE: apologies for poor links...How to remove formalin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Robert, dear all,

I am sorry for the wrong links you might find if using the Internet-links
given in my previous mail which also was sent out to the listserver.

Unfortunately you will be directed to a page with "German" text, stating
that ALL LINKS of the RKI(Robert Koch Institute, Berlin have been subjected
to link-address changes....(due to a new Content Management System)....

Unfortunately it seems there is no possibility to get the correct site(s)
by means of pasting the URL's of the English-Version-documents

But perhaps you can try the following: } German { page,
Find the link "ENGLISH" (left lower corner) and set
"rapid EM" as the search phrase....perhaps then you will get linked with
the pages I recommended in the mail before.
At least I was able to retrieve the documents (esp. No 18....which really
has the URL
http://www.rki.de/cln_011/nn_231622/EN/Content/Institute/DepartmentsUnit
s/NRC/CONSULAB/download__list__04.html__nnn=true ) that way again....

Regards
Wolfgang


----------
Von: derby-at-nmt.edu[SMTP:derby-at-nmt.edu]
Antwort an: derby-at-nmt.edu
Gesendet: Donnerstag, 13. Oktober 2005 17:52
An: W.Muss-at-salk.at
Betreff: [Microscopy] How to remove formalin




------------------------------------------------------------------------
----
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Greetings to all,

I am a bit stumped.
I have been asked to find a way to remove formalin, from formalin fixed
viruses.
Any tips/trick/methods would be appreciated.

Thank you,

Robert Derby
New Mexico Tech.


==============================Original
Headers==============================
5, 16 -- From derby-at-nmt.edu Thu Oct 13 10:46:56 2005
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5, 16 -- Received: from [129.138.14.28] (robert.nmt.edu [129.138.14.28])
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-0600
5, 16 -- User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.02.2022
5, 16 -- Date: Thu, 13 Oct 2005 09:47:45 -0600
5, 16 -- Subject: How to remove formalin
5, 16 -- From: Robert {derby-at-nmt.edu}
5, 16 -- To: {Microscopy-at-msa.microscopy.com}
5, 16 -- Message-ID: {BF73DDC1.357%derby-at-nmt.edu}
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==============================Original Headers==============================
20, 28 -- From W.Muss-at-salk.at Thu Oct 13 11:45:04 2005
20, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9])
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20, 28 -- Message-ID: {01C5D026.34E22E20.W.Muss-at-salk.at}
20, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at}
20, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at}
20, 28 -- To: "'derby-at-nmt.edu'" {derby-at-nmt.edu}
20, 28 -- Cc: "'Microscopy-at-msa.microscopy.com'" {Microscopy-at-msa.microscopy.com}
20, 28 -- Subject: AW: [Microscopy] RE: apologies for poor links...How to remove formalin
20, 28 -- Date: Thu, 13 Oct 2005 18:44:55 +0200
20, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at}
20, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor
20, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211
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From: jquinn-at-www.matscieng.sunysb.edu
Date: Thu, 13 Oct 2005 11:49:58 -0500
Subject: [Microscopy] re: Anew Platform..........

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is not specifically a microscopy job, but it could be and there are
members of the microscopy community with overlapping interests, so I
thought that I would forward this to the listserver from Brian Holloway.
Please do not contact me.
-Scott


Here is their announcement:
The Department of Applied Science at the College of William
and Mary, an interdisciplinary PhD-focused department
established in 1995, invites applicants for a tenure-track
position at the assistant professor level in biophysics,
neurophysiology, biomedical engineering, biomaterials,
or a related field, emphasizing either computational or
experimental approaches. The new faculty member will be
expected to establish a vigorous, independent and wellfunded
graduate research program at the interface of the
physical, mathematical, and biological sciences. Excellence and high
commitment to the
teaching of graduate and undergraduate students is also expected of all
faculty at the College.
Located two hours south of Washington, D.C. in Williamsburg, Virginia,
the College of William
and Mary is the second-oldest university in the United States and it was
recently names by the
editors of Newsweek Magazine as the "hottest small state school" in the
nation.

Candidates should submit a complete curriculum vitae, contact
information for three letters of reference, and copies of no more than
five refereed publications to: Faculty Search Committee,
Department of Applied Science, The College of William & Mary,
PO Box 8795, Williamsburg, VA 23187-8795. Review of materials
is expected to begin January 1, 2006 and will continue until the
position is filled.

The College is an EEO/AA employer.
Chartered in 1693 by the King and Queen of England,
William and Mary has approximately 5000
undergraduates and 2300 graduate and professional
students in 19 advanced degree programs. Applied
Science is an interdisciplinary graduate department
that offers M.S. and Ph.D. degrees. In addition to
the core faculty of the Department of Applied Science,
faculty from the Departments of Biology, Chemistry,
Computer Science, Mathematics, and Physics as well
as from NASA's Langley Research Center, DoE's Jefferson Lab, and local
industry participate
under various levels of affiliation.
http://www.as.wm.edu

-----Original Message-----
X-from: Brian Holloway [mailto:holloway-at-AS.WM.EDU]
Sent: Thursday, October 13, 2005 8:49 AM
To: holloway-at-as.wm.edu


Shu-You Li

I believe that your intent maybe honest,
however, the impact will be negative.

You would need a greater track-record
before attempting to usurp this listserver.

regards,

JQuinn

} From mail-at-ns.microscopy.com Thu Oct 13 10:52:22 2005
} Date: Thu, 13 Oct 2005 09:56:30 -0500
} To: jquinn-at-www.matscieng.sunysb.edu
} From: syli-at-northwestern.edu
} Reply-to: syli-at-northwestern.edu
} X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} Subject: [Microscopy] Anew platform for microscopists to discuss microscopy online
} Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} X-lewp: MicroscopyListSpam NAGS
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear list,
}
} I just created a new platform for us to discuss microscopy online using
} Web Wiz Forums. It might be a better platform than listservers, especially
} for those who want to reduce unnecessary emails. (I am not intending to
} say this listserv is bad. In fact Nestor did a great job and the
} listserv has served us for more than 10 years.) With the new online
} forum you can still set email notification for the topics you are
} interested in, at the same time you may ignore the topics out of
} interest.
}
} Other features of this online discussion board include:
} ***You need NOT register to post messages in most of the forums. However
} you have to type in your name every time you post a message. Registered
} users have full access to the website contents and functionality.
} ***Students or any Microscopists may ask questions related to microscopy
} in the Discussion board.
} ***Employers may post vacant positions in the Jobs & Resumes.
} ***Job-hunters are welcome to post their resumes too.
} ***Users may post and discuss their recent publications in
} recommended Readings.
} ***Open facilities or EM consulting companies may post their contact
} information in EM facilities nearby.
} ***Software authors may post their beta tests or freewares/sharewares in
} Software downloads.
} ***Commercial companies may post their product information and contact
} information free in EM companies
} ***I am collecting Daily Operation Manuals for all kinds of microscopes.
} Please send me your contributions to me. I will include your name as
} courtesy. After I have collected sufficient amount of manuals I will put
} them here so our users may benefit from your kindness.
} ***Finally but not least, you may create your own customized interest
} group in Customized Discussion Groups. You may set it as
} access-by-authorization-only or open to everyone.
}
} The link to this forum is
} http://www.ShuyouLi.com/
}
} I welcome all kinds of comments, suggestions and criticisms - to make
} us microscopists feel more convenient to discuss virtually world wide.
}
} Thanks,
} Shuyou Li
}
}
} _____________________________
} Shu-You Li, Ph.D.
} Electron Microscopist, EPIC
} NUANCE
} Northwestern University
} 2220 Campus Drive, 1161 Cook Hall (#2036 for mail)
} Evanston, IL 60208-3108, USA
} Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573
} Email: syli-at-northwestern.edu; shuyouli-at-gmail.com
} http://www.nuance.northwestern.edu/EPIC
} http://www.shuyouli.com
}
}
}
} ==============================Original Headers==============================
} 10, 16 -- From syli-at-northwestern.edu Thu Oct 13 09:55:41 2005
} 10, 16 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57])
} 10, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DEtfPA008880
} 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:41 -0500
} 10, 16 -- Received: from [129.105.37.16] (andromeda.ms.northwestern.edu [129.105.37.16])
} 10, 16 -- by merle.it.northwestern.edu (Postfix) with ESMTP id EE7109E847
} 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:40 -0500 (CDT)
} 10, 16 -- Date: Thu, 13 Oct 2005 09:57:37 -0500
} 10, 16 -- From: Shu-You Li {syli-at-northwestern.edu}
} 10, 16 -- To: microscopy-at-microscopy.com
} 10, 16 -- Subject: Anew platform for microscopists to discuss microscopy online
} 10, 16 -- Message-Id: {20051013095630.0382.SYLI-at-northwestern.edu}
} 10, 16 -- MIME-Version: 1.0
} 10, 16 -- Content-Type: text/plain; charset="US-ASCII"
} 10, 16 -- Content-Transfer-Encoding: 7bit
} 10, 16 -- X-Mailer: Becky! ver. 2.20 [en]
} ==============================End of - Headers==============================
}

==============================Original Headers==============================
7, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu Oct 13 11:49:58 2005
7, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33])
7, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DGnvQx009074
7, 12 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 11:49:57 -0500
7, 12 -- Received: (from jquinn-at-localhost)
7, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id j9DGjhF08025;
7, 12 -- Thu, 13 Oct 2005 12:45:43 -0400
7, 12 -- Date: Thu, 13 Oct 2005 12:45:43 -0400
7, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
7, 12 -- Message-Id: {200510131645.j9DGjhF08025-at-www.matscieng.sunysb.edu}
7, 12 -- To: microscopy-at-microscopy.com, syli-at-northwestern.edu
7, 12 -- Subject: re: Anew Platform..........
==============================End of - Headers==============================




From: syli-at-northwestern.edu
Date: Thu, 13 Oct 2005 12:46:31 -0500
Subject: [Microscopy] Re: Anew Platform..........

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you for your comments, Quinn and Ron-

I agree that I have much less track-record than Nestor or maybe anyone
else on the list. And I will be more than happy to terminate this forum
if MSA or another big name could have similiar bbs, or could I work
together with Nestor to create an alternative. Anyway I am providing an
option to listers.

Personally I will keep myself in this list for sure as long as we have
posts here. And I will keep my eye open on the forum I created. If you
think a web-based forum is better than email communications, I
appreciate it. If no one is willing to go there, that is fine with me
too.

BTW, Thanks for those who pointed out the broken link on registration
page. I have corrected it.

Shuyou


----------------------- Original Message -----------------------
On Thu, 13 Oct 2005 12:45:43 -0400
Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} wrote:
}
} Shu-You Li
}
} I believe that your intent maybe honest,
} however, the impact will be negative.
}
} You would need a greater track-record
} before attempting to usurp this listserver.
}
} regards,
}
} JQuinn
}
} } From mail-at-ns.microscopy.com Thu Oct 13 10:52:22 2005
} } Date: Thu, 13 Oct 2005 09:56:30 -0500
} } To: jquinn-at-www.matscieng.sunysb.edu
} } From: syli-at-northwestern.edu
} } Reply-to: syli-at-northwestern.edu
} } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} } Subject: [Microscopy] Anew platform for microscopists to discuss microscopy online
} } Errors-To: MicroscopyListSpamFilter-at-microscopy.com
} } X-lewp: MicroscopyListSpam NAGS
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Dear list,
} }
} } I just created a new platform for us to discuss microscopy online using
} } Web Wiz Forums. It might be a better platform than listservers, especially
} } for those who want to reduce unnecessary emails. (I am not intending to
} } say this listserv is bad. In fact Nestor did a great job and the
} } listserv has served us for more than 10 years.) With the new online
} } forum you can still set email notification for the topics you are
} } interested in, at the same time you may ignore the topics out of
} } interest.
} }
} } Other features of this online discussion board include:
} } ***You need NOT register to post messages in most of the forums. However
} } you have to type in your name every time you post a message. Registered
} } users have full access to the website contents and functionality.
} } ***Students or any Microscopists may ask questions related to microscopy
} } in the Discussion board.
} } ***Employers may post vacant positions in the Jobs & Resumes.
} } ***Job-hunters are welcome to post their resumes too.
} } ***Users may post and discuss their recent publications in
} } recommended Readings.
} } ***Open facilities or EM consulting companies may post their contact
} } information in EM facilities nearby.
} } ***Software authors may post their beta tests or freewares/sharewares in
} } Software downloads.
} } ***Commercial companies may post their product information and contact
} } information free in EM companies
} } ***I am collecting Daily Operation Manuals for all kinds of microscopes.
} } Please send me your contributions to me. I will include your name as
} } courtesy. After I have collected sufficient amount of manuals I will put
} } them here so our users may benefit from your kindness.
} } ***Finally but not least, you may create your own customized interest
} } group in Customized Discussion Groups. You may set it as
} } access-by-authorization-only or open to everyone.
} }
} } The link to this forum is
} } http://www.ShuyouLi.com/
} }
} } I welcome all kinds of comments, suggestions and criticisms - to make
} } us microscopists feel more convenient to discuss virtually world wide.
} }
} } Thanks,
} } Shuyou Li
} }
} }
} } _____________________________
} } Shu-You Li, Ph.D.
} } Electron Microscopist, EPIC
} } NUANCE
} } Northwestern University
} } 2220 Campus Drive, 1161 Cook Hall (#2036 for mail)
} } Evanston, IL 60208-3108, USA
} } Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573
} } Email: syli-at-northwestern.edu; shuyouli-at-gmail.com
} } http://www.nuance.northwestern.edu/EPIC
} } http://www.shuyouli.com
} }
} }
} }
} } ==============================Original Headers==============================
} } 10, 16 -- From syli-at-northwestern.edu Thu Oct 13 09:55:41 2005
} } 10, 16 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57])
} } 10, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DEtfPA008880
} } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:41 -0500
} } 10, 16 -- Received: from [129.105.37.16] (andromeda.ms.northwestern.edu [129.105.37.16])
} } 10, 16 -- by merle.it.northwestern.edu (Postfix) with ESMTP id EE7109E847
} } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 09:55:40 -0500 (CDT)
} } 10, 16 -- Date: Thu, 13 Oct 2005 09:57:37 -0500
} } 10, 16 -- From: Shu-You Li {syli-at-northwestern.edu}
} } 10, 16 -- To: microscopy-at-microscopy.com
} } 10, 16 -- Subject: Anew platform for microscopists to discuss microscopy online
} } 10, 16 -- Message-Id: {20051013095630.0382.SYLI-at-northwestern.edu}
} } 10, 16 -- MIME-Version: 1.0
} } 10, 16 -- Content-Type: text/plain; charset="US-ASCII"
} } 10, 16 -- Content-Transfer-Encoding: 7bit
} } 10, 16 -- X-Mailer: Becky! ver. 2.20 [en]
} } ==============================End of - Headers==============================
} }

--------------------------------------------------------------------------


==============================Original Headers==============================
10, 19 -- From syli-at-northwestern.edu Thu Oct 13 12:46:31 2005
10, 19 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57])
10, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9DHkUMh023704
10, 19 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 12:46:31 -0500
10, 19 -- Received: from [129.105.37.16] (andromeda.ms.northwestern.edu [129.105.37.16])
10, 19 -- by merle.it.northwestern.edu (Postfix) with ESMTP id B6E9B9E854;
10, 19 -- Thu, 13 Oct 2005 12:46:30 -0500 (CDT)
10, 19 -- Date: Thu, 13 Oct 2005 12:48:27 -0500
10, 19 -- From: Shu-You Li {syli-at-northwestern.edu}
10, 19 -- To: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
10, 19 -- Subject: Re: Anew Platform..........
10, 19 -- Cc: microscopy-at-microscopy.com
10, 19 -- In-Reply-To: {200510131645.j9DGjhF08025-at-www.matscieng.sunysb.edu}
10, 19 -- References: {200510131645.j9DGjhF08025-at-www.matscieng.sunysb.edu}
10, 19 -- Message-Id: {20051013123019.038B.SYLI-at-northwestern.edu}
10, 19 -- MIME-Version: 1.0
10, 19 -- Content-Type: text/plain; charset="US-ASCII"
10, 19 -- Content-Transfer-Encoding: 7bit
10, 19 -- X-Mailer: Becky! ver. 2.20 [en]
==============================End of - Headers==============================




From: frank.karl-at-degussa.com
Date: Thu, 13 Oct 2005 13:20:37 -0500
Subject: [Microscopy] TEM and precipitated silica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





(Lurk mode to off)

Hello everyone,

Recently I have been examining precipitated silica with the TEM (80 KV) and
have observed a complication. First I thought my scope was acting up (well
it is a little) and giving me unstable magnification. I would examine a
particle at one mag, go to a different magnification, maybe a third, come
back to the original magnification and fins my particle has grown. It
appeared to have lost detail too. Almost like popcorn expanding.

I spend a lot of time looking at calibration grids, changing magnification,
spot size and stigmating to beat the band and convinced myself the scope
was not changing magnification on me.

I prepped new samples, found a nice silica agglomerate and took a photo. I
left the beam on in and took simply took photos every once and awhile (
about every 5-8 minutes) and my little fine grain agglomerate blew up like
popcorn.

I suspect it's the chloroform I use to grind, ultrasonicate and disperse
in. The silica hold trace levels of chloroform due to H-bonding until the
beam cooks it out which causes the particle to expand, changing the shape
and size of the particle.

So... Has anyone else had this experience and more importantly does anyone
have a better method of prepping predicated silica for TEM analysis?

Thanks in advance!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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==============================Original Headers==============================
18, 17 -- From frank.karl-at-degussa.com Thu Oct 13 13:20:37 2005
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==============================End of - Headers==============================




From: avklaus-at-amnh.org
Date: Thu, 13 Oct 2005 13:26:35 -0500
Subject: [Microscopy] How to remove formalin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Robert,

The below reference describes the use of critical point drying for formalin
removal from tissues for subsequent DNA extraction.

Formalin Removal from Archival Tissue by Critical Point Drying
Sheng-Guo Fang, Qiu-Hong Wan, and Noboru Fujihara
BioTechniques Vol. 33, No. 3: pp 604-611 (Sep 2002)

Best regards,

Angela

Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA

Tel: 212-769-5977
Email: avklaus-at-amnh.org

-----Original Message-----
X-from: derby-at-nmt.edu [mailto:derby-at-nmt.edu]
Sent: Thursday, October 13, 2005 11:47 AM
To: avklaus-at-amnh.org

Greetings to all,

I am a bit stumped.
I have been asked to find a way to remove formalin, from formalin fixed
viruses.
Any tips/trick/methods would be appreciated.

Thank you,

Robert Derby
New Mexico Tech.


==============================Original Headers==============================
5, 16 -- From derby-at-nmt.edu Thu Oct 13 10:46:56 2005
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5, 16 -- User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.02.2022
5, 16 -- Date: Thu, 13 Oct 2005 09:47:45 -0600
5, 16 -- Subject: How to remove formalin
5, 16 -- From: Robert {derby-at-nmt.edu}
5, 16 -- To: {Microscopy-at-msa.microscopy.com}
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==============================Original Headers==============================
17, 28 -- From avklaus-at-amnh.org Thu Oct 13 13:26:33 2005
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17, 28 -- To: {derby-at-nmt.edu}
17, 28 -- Cc: {Microscopy-at-msa.microscopy.com}
17, 28 -- Subject: RE: [Microscopy] How to remove formalin
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From: smalinskas-at-yahoo.com
Date: Thu, 13 Oct 2005 14:02:57 -0500
Subject: [Microscopy] Re: viaWWW: boundary grain of Wfilament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Vincent,

We need more information. What are you trying to
accomplish? Do you want to study grain boundary using
metallographic methods?

Stu Smalinskas, P.E.
Metallurgist
SKF USA
Plymouth, Michigan

--- vincent.metzger-at-philips.com wrote:

}
} Question: I would like to study the boundary grain
} of Wfilament of lamp. I'm searching a way to prepare
} the sample to make good observation of the grain
} boundary.


} Email: vincent.metzger-at-philips.com
} Name: vincent metzger
} Organization: philips
} Title-Subject: [Filtered] lamps filament preparation



__________________________________
Yahoo! Music Unlimited
Access over 1 million songs. Try it free.
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==============================Original Headers==============================
9, 19 -- From smalinskas-at-yahoo.com Thu Oct 13 14:02:56 2005
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9, 19 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
9, 19 -- Subject: Re: [Microscopy] viaWWW: boundary grain of Wfilament
9, 19 -- To: vincent.metzger-at-philips.com, microscopy-at-ns.microscopy.com
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From: syli-at-northwestern.edu
Date: Thu, 13 Oct 2005 14:35:48 -0500
Subject: [Microscopy] Anew Platform..........

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm with Ron on this one.

Give me a managed, moderated listserver any day, I have no wish to sift
through the illiterate, ignorant ramblings which are likely to dominate for the
occasional speck of good information.

Remember all that stuff about that phony 'revolutionary' microscope -- what
was it -- the Rolfe Royale, or somesuch?

"...........why has the scientific community suppressed this important
invention?" etc, etc, etc.

What's this past tense doing in relation to Nestor, anyway?

Just an accidental grammatical imperfection, I hope.

cheers

rtch




Date sent: Thu, 13 Oct 2005 11:03:05 -0500
To: r.sims-at-auckland.ac.nz
X-from: randerson20-at-tampabay.rr.com
Send reply to: randerson20-at-tampabay.rr.com

I have received a couple of emails off list on this topic. Most of them
are fairly pertinent. I appreciate veyr much.

When comparing BBS with email listservs, we must think collectively
their pros and cons. An advantage of listserv is that we get posts in
regular email inbox so we don't need to make special effort to visit BBS,
and we are not getting too much emails from MSA. With the wonderful spam
filtration by Nestor, we get 20-30 emails all relevant to mciroscopy
everyday. It is not big deal as we receive hundreds of emails daily.

The shortage of listserv is classification and archiving. We ussually
find it difficult to find information in old communications although
Nestor has all emails archived from 1993.

Talking about the mixture of regular emails with listserv posts, it is
advantageous but also can be shortcoming. I don't know how others do but
I myself have to set a email filter to sort MSA emails to a certain
folder and, if there is no very interesting topic, I will read these
emails only when I have coffee break. It is no difference for me to go
check a website or read special MSA email folder at leisure time. Yes we
are getting not too much emails from MSA, but we should also consider
those communications off list, as those I received today on this topic
offline. Some of these communications are trully fruitful and worth
accessible by others. With BBS we don't need worry spamming the "list".

I think the key issue here is whether the topic is time-sensitive. If
the topics we are discussing need immediate response and we need never
check back the topic after the discussion, then listserv is great. For
the topics that are not time-sensitve, for example the pay-per-play EM
serivices someone asked here the other day, it might be benificial to
others who might need this information months later. In addition to
information sharing, BBS is also good on file sharing, software programs,
manuals, papers, product application notes, etc. All these kinds of
information can be saved collectively on a easy to reach website.

Although I am keen to see a success of this website, I will face the
truth and see how it goes in several months.

Thanks to all who have replied to this topic,
Shuyou
_____________________________
Shu-You Li, Ph.D.
Electron Microscopist, EPIC
NUANCE
Northwestern University
2220 Campus Drive, 1161 Cook Hall (#2036 for mail)
Evanston, IL 60208-3108, USA
Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573
Email: syli-at-northwestern.edu; shuyouli-at-gmail.com
http://www.nuance.northwestern.edu/EPIC
http://www.shuyouli.com



==============================Original Headers==============================
9, 16 -- From syli-at-northwestern.edu Thu Oct 13 14:35:48 2005
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9, 16 -- Date: Thu, 13 Oct 2005 14:37:44 -0500
9, 16 -- From: Shu-You Li {syli-at-northwestern.edu}
9, 16 -- To: microscopy-at-microscopy.com
9, 16 -- Subject: re: [Microscopy] re: Anew Platform..........
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From: klivi-at-jhu.edu
Date: Thu, 13 Oct 2005 14:41:02 -0500
Subject: [Microscopy] TEM Al-Ni foil cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Does someone know of a gentle chemical etch that could be used on an
ion milled sample of Al and Ni (not alloyed) to clean its surface
(i.e., removal of ion mill contamination)?
Ciao for now,
Ken
--
Kenneth JT Livi, Ph.D.
Department of Earth and Planetary Sciences
3400 N. Charles St.
Johns Hopkins University
Baltimore, MD 21218
(410) 516-8342
(410) 516-7933 fax

==============================Original Headers==============================
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From: Sally.Stowe-at-anu.edu.au
Date: Thu, 13 Oct 2005 18:47:15 -0500
Subject: [Microscopy] RE: Anew platform for microscopists to discuss microscopy online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Shu-you,
You seem to be copping a fair bit of flack for something which has obviously
taken a lot of thought and work. I think you are to be congratulated and
wish you luck, your site fills a real need, and augments rather than
"replaces" this listserver.

The problem I see is that it will take a lot of continuing work to keep
current. Many people have started, in a rush of enthusiasm, without really
achieving the critical mass and longevity needed to become "part of the
furniture" as are this listserver and the confocal equivalent. The
University of Florida "tips and tricks" site
http://www.biotech.ufl.edu/EM/tips/sem.html has the highest profile I know
of, and I visit it. Yours is more ambitious, but if you can succeed you
will give us all an important resource.

So all the best!

Sally


Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
ANU CRICOS#00120C



} -----Original Message-----
} From: syli-at-northwestern.edu [mailto:syli-at-northwestern.edu]
} Sent: Friday, 14 October 2005 12:56 AM
} To: sally.stowe-at-anu.edu.au
} Subject: [Microscopy] Anew platform for microscopists to
} discuss microscopy online
}
}
}
}
}
} ---------------------------------------------------------------
} -------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver



==============================Original Headers==============================
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11, 27 -- To: {syli-at-northwestern.edu} , {microscopy-at-microscopy.com}
11, 27 -- Subject: RE: [Microscopy] Anew platform for microscopists to discuss microscopy online
11, 27 -- Date: Fri, 14 Oct 2005 09:46:56 +1000
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From: syli-at-northwestern.edu
Date: Thu, 13 Oct 2005 19:33:38 -0500
Subject: [Microscopy] Re: Anew platform for microscopists to discuss microscopy online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for the encouragement. I will keep this site in good shape with
continuous efforts. If necessary I will of course invite volunteers to
moderate the site together with me to keep fresh blood.

To avoid "splinter" answer-seekers and resources of the society, I put a
sticky note in the discussion board to inform new comers try this
listserv if they could not get answer from the website. In this way I
hope the website can be a compensation to the listserv but not
competition.

Some of you pointed out that I should have discussed this attempt BEFORE
creating or annoucing the presence of the site. I do appologize if the
website is totally unacceptable for some of the listers here.

With regards,
Shuyou

----------------------- Original Message -----------------------
On Fri, 14 Oct 2005 09:46:56 +1000
"Sally Stowe" {Sally.Stowe-at-anu.edu.au} wrote:
} Hi Shu-you,
} You seem to be copping a fair bit of flack for something which has obviously
} taken a lot of thought and work. I think you are to be congratulated and
} wish you luck, your site fills a real need, and augments rather than
} "replaces" this listserver.
}
} The problem I see is that it will take a lot of continuing work to keep
} current. Many people have started, in a rush of enthusiasm, without really
} achieving the critical mass and longevity needed to become "part of the
} furniture" as are this listserver and the confocal equivalent. The
} University of Florida "tips and tricks" site
} http://www.biotech.ufl.edu/EM/tips/sem.html has the highest profile I know
} of, and I visit it. Yours is more ambitious, but if you can succeed you
} will give us all an important resource.
}
} So all the best!
}
} Sally
}
}
} Dr SJ Stowe
} Facility Coordinator
} ANU Electron Microscopy Unit
} ANU CRICOS#00120C
}
}
}
} } -----Original Message-----
} } From: syli-at-northwestern.edu [mailto:syli-at-northwestern.edu]
} } Sent: Friday, 14 October 2005 12:56 AM
} } To: sally.stowe-at-anu.edu.au
} } Subject: [Microscopy] Anew platform for microscopists to
} } discuss microscopy online
} }
} }
} }
} }
} }
} } ---------------------------------------------------------------
} } -------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy
} } Society of America To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ---------------------------------------------------------------
} } -------------
} }
} } Dear list,
} }
} } I just created a new platform for us to discuss microscopy
} } online using Web Wiz Forums. It might be a better platform
} } than listservers, especially for those who want to reduce
} } unnecessary emails. (I am not intending to say this listserv
} } is bad. In fact Nestor did a great job and the listserv has
} } served us for more than 10 years.) With the new online forum
} } you can still set email notification for the topics you are
} } interested in, at the same time you may ignore the topics out
} } of interest.
} }
} } Other features of this online discussion board include:
} } ***You need NOT register to post messages in most of the
} } forums. However you have to type in your name every time you
} } post a message. Registered users have full access to the
} } website contents and functionality. ***Students or any
} } Microscopists may ask questions related to microscopy in the
} } Discussion board. ***Employers may post vacant positions in
} } the Jobs & Resumes. ***Job-hunters are welcome to post their
} } resumes too. ***Users may post and discuss their recent
} } publications in recommended Readings. ***Open facilities or EM
} } consulting companies may post their contact information in EM
} } facilities nearby. ***Software authors may post their beta
} } tests or freewares/sharewares in Software downloads.
} } ***Commercial companies may post their product information and
} } contact information free in EM companies ***I am collecting
} } Daily Operation Manuals for all kinds of microscopes. Please
} } send me your contributions to me. I will include your name as
} } courtesy. After I have collected sufficient amount of manuals
} } I will put them here so our users may benefit from your
} } kindness. ***Finally but not least, you may create your own
} } customized interest group in Customized Discussion Groups. You
} } may set it as access-by-authorization-only or open to everyone.
} }
} } The link to this forum is
} } http://www.ShuyouLi.com/
} }
} } I welcome all kinds of comments, suggestions and criticisms -
} } to make us microscopists feel more convenient to discuss
} } virtually world wide.
} }
} } Thanks,
} } Shuyou Li
} }
} }
} } _____________________________
} } Shu-You Li, Ph.D.
} } Electron Microscopist, EPIC
} } NUANCE
} } Northwestern University
} } 2220 Campus Drive, 1161 Cook Hall (#2036 for mail)
} } Evanston, IL 60208-3108, USA
} } Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573
} } Email: syli-at-northwestern.edu; shuyouli-at-gmail.com
} } http://www.nuance.northwestern.edu/EPIC
} } http://www.shuyouli.com
} }
} }
} }
} } ==============================Original
} } Headers==============================
} } 10, 16 -- From syli-at-northwestern.edu Thu Oct 13 09:55:41 2005
} } 10, 16 -- Received: from merle.it.northwestern.edu
} } (merle.it.northwestern.edu [129.105.16.57])
} } 10, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with
} } ESMTP id j9DEtfPA008880
} } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct
} } 2005 09:55:41 -0500
} } 10, 16 -- Received: from [129.105.37.16]
} } (andromeda.ms.northwestern.edu [129.105.37.16])
} } 10, 16 -- by merle.it.northwestern.edu (Postfix) with
} } ESMTP id EE7109E847
} } 10, 16 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct
} } 2005 09:55:40 -0500 (CDT)
} } 10, 16 -- Date: Thu, 13 Oct 2005 09:57:37 -0500
} } 10, 16 -- From: Shu-You Li {syli-at-northwestern.edu}
} } 10, 16 -- To: microscopy-at-microscopy.com
} } 10, 16 -- Subject: Anew platform for microscopists to discuss
} } microscopy online 10, 16 -- Message-Id:
} } {20051013095630.0382.SYLI-at-northwestern.edu}
} } 10, 16 -- MIME-Version: 1.0
} } 10, 16 -- Content-Type: text/plain; charset="US-ASCII"
} } 10, 16 -- Content-Transfer-Encoding: 7bit
} } 10, 16 -- X-Mailer: Becky! ver. 2.20 [en]
} } ==============================End of -
} } Headers==============================
} }

--------------------------------------------------------------------------


==============================Original Headers==============================
7, 19 -- From syli-at-northwestern.edu Thu Oct 13 19:33:38 2005
7, 19 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57])
7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id j9E0XcRL031991
7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2005 19:33:38 -0500
7, 19 -- Received: from [129.105.37.16] (andromeda.ms.northwestern.edu [129.105.37.16])
7, 19 -- by merle.it.northwestern.edu (Postfix) with ESMTP id BB9889E844;
7, 19 -- Thu, 13 Oct 2005 19:33:37 -0500 (CDT)
7, 19 -- Date: Thu, 13 Oct 2005 19:35:34 -0500
7, 19 -- From: Shu-You Li {syli-at-northwestern.edu}
7, 19 -- To: {Sally.Stowe-at-anu.edu.au}
7, 19 -- Subject: Re: [Microscopy] Anew platform for microscopists to discuss microscopy online
7, 19 -- Cc: {microscopy-at-microscopy.com}
7, 19 -- In-Reply-To: {000101c5d050$6537bd40$9024cb96-at-rsbs.anu.edu.au}
7, 19 -- References: {200510131455.j9DEtm4b009076-at-ns.microscopy.com} {000101c5d050$6537bd40$9024cb96-at-rsbs.anu.edu.au}
7, 19 -- Message-Id: {20051013192159.0397.SYLI-at-northwestern.edu}
7, 19 -- MIME-Version: 1.0
7, 19 -- Content-Type: text/plain; charset="US-ASCII"
7, 19 -- Content-Transfer-Encoding: 7bit
7, 19 -- X-Mailer: Becky! ver. 2.20 [en]
==============================End of - Headers==============================




From: gary-at-gaugler.com
Date: Thu, 13 Oct 2005 19:49:22 -0500
Subject: [Microscopy] Re: Anew platform for microscopists to discuss

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is nice but I don't think that it will stand up
to the test of time.

Your GUI is good but the problem is that one
has to wonder how long you will be around relative
to Nestor. You are of a different venue than Nestor.

I'm betting on Nestor.

gary g.


At 07:57 AM 10/13/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


==============================Original Headers==============================
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10, 25 -- Date: Thu, 13 Oct 2005 17:47:16 -0700
10, 25 -- To: syli-at-northwestern.edu
10, 25 -- From: Gary Gaugler {gary-at-gaugler.com}
10, 25 -- Subject: Re: [Microscopy] Anew platform for microscopists to discuss
10, 25 -- microscopy online
10, 25 -- Cc: MSA listserver {microscopy-at-microscopy.com}
10, 25 -- In-Reply-To: {200510131457.j9DEvcWG012187-at-ns.microscopy.com}
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From: syli-at-northwestern.edu
Date: Thu, 13 Oct 2005 20:02:14 -0500
Subject: [Microscopy] Re: Anew platform for microscopists to discuss microscopy online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If most of us agree that BBS is a better technology than listserv, I
don't think we are lack of volunteers to maintain the forum. You are
right "time can prove all".

Thanks for the comment, anyway.
Shuyou

----------------------- Original Message -----------------------
On Thu, 13 Oct 2005 17:47:16 -0700
Gary Gaugler {gary-at-gaugler.com} wrote:
} This is nice but I don't think that it will stand up
} to the test of time.
}
} Your GUI is good but the problem is that one
} has to wonder how long you will be around relative
} to Nestor. You are of a different venue than Nestor.
}
} I'm betting on Nestor.
}
} gary g.
}
}
} At 07:57 AM 10/13/2005, you wrote:
}
}
}
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Dear list,
} }
} } I just created a new platform for us to discuss microscopy online using
} } Web Wiz Forums. It might be a better platform than listservers, especially
} } for those who want to reduce unnecessary emails. (I am not intending to
} } say this listserv is bad. In fact Nestor did a great job and the
} } listserv has served us for more than 10 years.) With the new online
} } forum you can still set email notification for the topics you are
} } interested in, at the same time you may ignore the topics out of
} } interest.
} }
} } Other features of this online discussion board include:
} } ***You need NOT register to post messages in most of the forums. However
} } you have to type in your name every time you post a message. Registered
} } users have full access to the website contents and functionality.
} } ***Students or any Microscopists may ask questions related to microscopy
} } in the Discussion board.
} } ***Employers may post vacant positions in the Jobs & Resumes.
} } ***Job-hunters are welcome to post their resumes too.
} } ***Users may post and discuss their recent publications in
} } recommended Readings.
} } ***Open facilities or EM consulting companies may post their contact
} } information in EM facilities nearby.
} } ***Software authors may post their beta tests or freewares/sharewares in
} } Software downloads.
} } ***Commercial companies may post their product information and contact
} } information free in EM companies
} } ***I am collecting Daily Operation Manuals for all kinds of microscopes.
} } Please send me your contributions to me. I will include your name as
} } courtesy. After I have collected sufficient amount of manuals I will put
} } them here so our users may benefit from your kindness.
} } ***Finally but not least, you may create your own customized interest
} } group in Customized Discussion Groups. You may set it as
} } access-by-authorization-only or open to everyone.
} }
} } The link to this forum is
} } http://www.ShuyouLi.com/
} }
} } I welcome all kinds of comments, suggestions and criticisms - to make
} } us microscopists feel more convenient to discuss virtually world wide.
} }
} } Thanks,
} } Shuyou Li
} }
} }
} } _____________________________
} } Shu-You Li, Ph.D.
} } Electron Microscopist, EPIC
} } NUANCE
} } Northwestern University
} } 2220 Campus Drive, 1161 Cook Hall (#2036 for mail)
} } Evanston, IL 60208-3108, USA
} } Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573
} } Email: syli-at-northwestern.edu; shuyouli-at-gmail.com
} } http://www.nuance.northwestern.edu/EPIC
} } http://www.shuyouli.com
} }
} }
} }
} } ==============================Original Headers==============================
} } 10, 16 -- From syli-at-northwestern.edu Thu Oct 13 09:55:41 2005
} } 10, 16 -- Received: from merle.it.northwestern.edu
} } (merle.it.northwestern.edu [129.105.16.57])
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} } j9DEtfPA008880
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} } 09:55:40 -0500 (CDT)
} } 10, 16 -- Date: Thu, 13 Oct 2005 09:57:37 -0500
} } 10, 16 -- From: Shu-You Li {syli-at-northwestern.edu}
} } 10, 16 -- To: microscopy-at-microscopy.com
} } 10, 16 -- Subject: Anew platform for microscopists to discuss
} } microscopy online
} } 10, 16 -- Message-Id: {20051013095630.0382.SYLI-at-northwestern.edu}
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} } ==============================End of - Headers==============================

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==============================Original Headers==============================
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5, 19 -- From: Shu-You Li {syli-at-northwestern.edu}
5, 19 -- To: Gary Gaugler {gary-at-gaugler.com}
5, 19 -- Subject: Re: [Microscopy] Anew platform for microscopists to discuss microscopy online
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From: U.J.Potter-at-ns.microscopy.com
Date: Fri, 14 Oct 2005 07:42:22 -0500
Subject: [Microscopy] viaWWW: online booking software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (U.J.Potter) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, October 14, 2005 at 04:08:26
---------------------------------------------------------------------------

Email: U.J.Potter
Name: Ursula Potter

Organization: University of Bath

Title-Subject: [Filtered] Online Booking Software for EM & LM facilities

Question: Dear all,

I would appreciate any information or user comments from those who use online booking software. I am searching for a reasonably priced system for University-wide users that enables them to book Electron Microscope, Confocal and FACs equipment housed in a central unit. I have also come across a system called phpScheduleIt which seems to be freeware although I don't quite understand how this works - any comments on this would be very helpful.

Thanks
Ursula
-----------

---------------------------------------------------------------------------

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8, 12 -- From: U.J.Potter-at-ns.microscopy.com (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: online booking software
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: Geoffrey_Williams-at-brown.edu
Date: Fri, 14 Oct 2005 10:02:59 -0500
Subject: [Microscopy] This new platform.... (my POV)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,

I wanted to weigh in on this subject (please bear with me). As a
preface: I've been a part of many forums, mostly automotive, a few other
ones and would be an enthusiastic supporter of a UBB type forum, and
also a long time Listserver subscriber (in various locations).

In fact its an idea that I've been letting roll around in my head for a
few years - sort of hoping with all that incubation the idea might
emerge as a pearl and take hold... no it hasn't emerged and I've been
beaten to the punch. ;)

Problem is: we are a small (relatively) group, and a significant number
of the microscopy community participates or subscribes to the list.
Honestly, and maybe statistics will prove me wrong but it feels like the
listserver is slightly decreasing in traffic. Maybe on par with the
decrease in participation in local area meetings (shame on you all who
don't make the effort to serve and participate in your LAS). There
could be many causes. Or it is just my perception. And yes, like many I
have a folder in my outlook that collects the listserver emails. I do
tend to read all of them, or at least a significant number and I enjoy
absorbing new information or perspectives or ways to explain aspects of
what we all do.

But a forum style discussion group is different. Different in that you
can have real-time posting/replies without the email going through a
main server. It would cut down the times when you get replies before
the email that you sent shows up in your inbox. It also can provide
(depending on the forum set up) a format that allows easy posting of
sample images, or diagrams. Often diagnosis is facilitated by images
and a forum can provide a place to archive and share those images in a
searchable database. On the flip side, if the forum succeeds it can
disable a modest server relatively quickly. I've been involved in the
growth of many forums. One now is the leading source for TDI
information (tdiclub.com). It started as a small group and has grown
immensely. It most likely far exceeds any numbers we could hope to
attain as a microscopy forum. But the model is sound. Provide a place
for experts and novices to have real time discussion, question and
answer, with the ability to easily archive and search.

In the ideal world I think the forum should be set up with the support
of good ol' Nestor and MSA. He's done so much to keep this listserver
going and obviously has a lot invested in it, as do we all who
participate. There are quite a few here who have been on longer than my
10 or so years (I think that's about right, between the different email
accounts and all). And that is a core of email junkies. But about 6-7
years ago UBB came out with a program that changed listservers and
newsgroups forever. There are many variations and I'm sure most
everyone has seen/participated in one type of forum or another. The
success of a forum is 100% dependent on the core users, and the
collective participation/knowledge. I believe a microscopy forum could
survive and do well and provide a valuable resource, and potentially
could easily exist in tandem with the listserver, maybe even with MT or
other publications.

Maybe Shuyou's forum is premature, maybe not. The data can easily be
converted to different forum structures. It interface is easily
modified and the archiving and searching is a breeze, depending on
software and server structure.

Also, important to make note. A Forum can be made as secure and private
(more so even) than a listserver. The administrator can grant access to
view the discussion threads to only registered users. They can set it
up to allow only registered users to post. They can also filter and
restrict and monitor requests for usernames. All that without a huge
amount of work. Okay there is a little bit of work, esp as the group
gets larger. However, a forum of 38,000 registered users is well
maintained by two administrators and a host of moderators. Typically one
moderator per topic/discussion area, and it would probably be reasonably
easy to agree upon one or two people with distinct experiences who could
easily moderate the content in say an SEM or TEM forum. Very simple,
very elegant.

My final words on this subject (for this email)... ;-)
I'm of the opinion that listservers are a dinosaur. Kind of like 667
polaroid film. Yes, they still work to convey information, but: there
are more modern methods to do the same thing, saving everyone on all
ends much time and space. No more deleting emails you don't want to
read - no more worring about finding the right topic, flame wars and
arguments about who knows what (like this) can be sequestered or allowed
to come to completion without filling everyone's inbox. Until there is
a viable alternative up on the 'net, I will faithfully read and
contribute to the Listserver in its current and future forms.

Just a few of my thoughts,

With deep respect for the Listserver and the members here,
Geoff


Geoff Williams
Leduc Bioimaging Facility Manager
Brown University

http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/



==============================Original Headers==============================
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From: scanning-at-fams.org
Date: Fri, 14 Oct 2005 13:55:21 -0500
Subject: [Microscopy] SCANNING 2006 Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Just a quick announcement inviting you all to attend SCANNING 2006,
Tuesday, April 25 through Thursday, April 27 in Washington, D.C. The
location is prime--just two doors down from The White House--and the
meeting promises to be an excellent scientific forum. We encourage
everyone to submit an abstract. THE DEADLINE FOR ABSTRACT SUBMISSION IS
JANUARY 18, 2006.

A preliminary program is below for your review and we hope to see you
there! Note the brand new course by Alan Boyde and new sessions being
held by Ken Moore (Morphology Core), Elaine Humphrey (Univ. of BC),
Robert Carlton (GlaxoSmithKline), Bev Giammara (Univ. of Louisville),
Terry Allen (Christie Hospital NHS Trust), Warren MoberlyChan (Lawrence
Livermore National Labs), Dale Newbury (NIST), Rob Apkarian (Emory
Univ.), and Bruno Frohlich (Smithsonian Institution).

To download a more comprehensive Preliminary Program including
summaries and the Registration Form or additional information, please
visit www.scanning.org.

Among the Sessions and Courses
SCANNING 2006—Washington, D.C., • April 25–27, 2006

Special New Course:

Skeletal Tissue Structural Biology and the Contribution and Potential
of the Scanning Microscopies–NEW!
Chair: Alan Boyde, Barts and the London School of Medicine and
Dentistry and Queen Mary University of London, London, U.K.
 
The Role of Scanning Microscopies in the Study of Disease—NEW SESSION!
Chair: Kenneth C. Moore, Morphology Core, Iowa City, Iowa, USA

Microwave in Microscopy–NEW SESSION!
Chairs: Elaine Humphrey, University of British Columbia, Vancouver, BC
and Beverly Giammara, University of Louisville School of Medicine,
Louisville, Kentucky, USA

SEM of Biomaterials and Biomedical Devices–NEW SESSION!
Chair: Robert Apkarian, Emory University, Atlanta, GA, USA

Applications of Environmental and Low Vacuum SEM in the Pharmaceutical
Industry—NEW SESSION!
Robert A. Carlton, GlaxoSmithKline, King of Prussia, PA, USA

Microscopies for the Structural and Dynamic Organization of the
Nucleus—NEW SESSION!
Chair: Terry D. Allen, Paterson Institute for Cancer Research, Christie
Hospital NHS Trust, Manchester, U.K.

Forensic Science with a Special GSR Segment
Chairs: S. Frank Platek, National Forensic Chemistry Center, U.S. Food
and Drug Administration; M.A. Trimpe, Hamilton County Coroner’s Office,
Cincinnati, OH, USA

Focused Ion Beam Microscopy
Chair: Warren J. MoberlyChan, Materials Science and Technology
Division, Lawrence Livermore National Laboratory, Livermore, CA, USA

Electron Beam/Specimen Interaction Workshop
Chair: Dr. Michael T. Postek, Leader, Nano Scale Metrology, National
Institute of Standards and Technology, Gaithersburg, MD, USA
 
The Electron Beam/Specimen Interaction Workshop has brought
experimentalists and modeling experts together for nearly a decade to
share information on this exciting topic.

Advances in Electron Beam Microanalysis of Individual Particles
Chair: Dale E. Newbury NIST, Gaithersburg, MD, USA

Biological and Biomedical Applications of Scanning Microscopy
Chair: Timothy Maugel, Laboratory for Biological Ultrastructure,
Biology Department, University of Maryland, College Park, MD, USA

Scanning Museum Objects: Research, Documentation, and Preservation
Chair: Bruno Frohlich, National Museum of Natural History, Smithsonian
Institution, Washington, D.C., USA

Among the Short Courses:

Tuesday, April 25

Scanning Microscopy in Forensic Science
Chairs: S. Frank Platek, National Forensic Chemistry Center, U.S. Food
and Drug Administration, Cincinnati, OH; Michael T. Postek, US
DOC-NIST, Gaithersburg, MD, M.A. Trimpe, Hamilton County Coroner’s
Office, Cincinnati, OH, USA, and Michael McVicar, Chemistry Section
Scientist, Center of Forensic Sciences, Ontario, Canada

Wednesday, April 26

Introduction to AFM—Sponsored by Pacific Nanotechnology, Inc. (Two-Day
Course)
Chair: Paul West, Pacific Nanotechnology, Inc., Tustin, CA, USA

Introduction to AFM (continued)—Sponsored by Pacific Nanotechnology,
Inc. (Two-Day Course)
Chair: Paul West, Pacific Nanotechnology, Inc., Tustin, CA, USA

Advanced Topics in SEM
Chairs: D.C. Joy, Department of Biochemistry, Cellular and Molecular
Biology, University of Tennessee, Knoxville, TN, USA; O. C. Wells,
Yorktown Heights, NY, USA

Thursday, April 27

Mastering the Digital Image–NEW COURSE!
Chair: J. Christian Russ, Reindeer Graphics, Asheville, NC, USA

Quantitative Measurements—Sponsored by Pacific Nanotechnology, Inc.
(Half Day Course)
Chair: Paul West, Pacific Nanotechnology, Inc., Tustin, CA, USA

Materials Sciences Applications—Sponsored by Pacific Nanotechnology,
Inc. (Half Day Course)
Chair: Paul West, Pacific Nanotechnology, Inc., Tustin, CA, USA

Best regards,


Phaedra McGuinness
Managing Editor
SCANNING, The Journal of Scanning Microscopies
P.O. Box 485
Mahwah, NJ 07430
Tel: (201) 818-1010 * Fax: (201) 818-0086 *email: scanning-at-fams.org *
Web: www.scanning.org


==============================Original Headers==============================
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31, 20 -- Subject: SCANNING 2006 Announcement
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From: dale_batchelor-at-ncsu.edu
Date: Fri, 14 Oct 2005 16:01:00 -0500
Subject: [Microscopy] AReMS 2005 Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear collegues,
You are invited to the Appalachian Regional Microscopy Society (AReMS)
2005 Fall Meeting at the Broyhill Inn and Conference Center, Boone, NC.

Theme: Nanoparticles in Biomedicine, Electronics & Material Science

Thursday, October 20, 2005

2:30-4:30 pm
Workshop: Optical Microscopy at Sub-100nm Resolution,  
Rene Salazar, Aetos Technologies, Inc.

2:30-4:30 pm
Workshop: Force Measurements and Pulling Using an Atomic Force
Microscope, Keith Jones, Asylum Research

6:00-7:00 pm
Social: Integon Room at the Broyhill Inn

7:00-8:00 pm
Dinner: Bernhardt Room at the Broyhill Inn

8:00-9:00 pm
Dinner talk by Keynote speaker: Dale Newbury, National Institute of
Standards & Technology: “Not Just a Pretty Picture: X-ray Mapping is 50
Years Young, the Best is Yet to Come, and the Future is Now!”


Friday, October 21, 2004, Powers North (formerly Trillium North) Room

8:00-8:10 am
Welcome, President Lou Germinario

8:15-8:45 am
James Wittig, Vanderbilt University: “Metallic and Semiconducting
Nanoparticles for Biomedical Applications”

8:50-9:20 am
Jonathan Bender, University of South Carolina: “Nanotribology Study of
Ultra-high Molecular Polyethylene”

9:25-9:55 am
Dale Newbury, National Institute of Standards & Technology: “Blunders
in Automatic Peak Identification of Major Constituents by
Electron-excited Energy Dispersive X-ray Microanalysis”

10:15-10:30 am
Introduction of Posters

10:35-11:05 am
Richard Spontak, NC State University: “Direct Visualization of
Polymer-Polymer Dewetting in the Presence of a Block Copolymer: From
Macroscopic to Microscopic Mechanisms”

11:10-11:25 am
Break and Posters Session

11:30-12:00 pm
Sarah White, Hitachi High Technology: "Low Voltage STEM Imaging and
Analysis of Catalysts, Nanoparticles, Nanomaterials, and Nanolayers"
 
For more information or to register online visit the website
www.arems.org
To register by phone call Sam Pennington at 704-825-8261 or by email at
shpennington-at-alliedhightech.com



==============================Original Headers==============================
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From: bucana-at-audumla.mdacc.tmc.edu
Date: Fri, 14 Oct 2005 16:44:15 -0500
Subject: [Microscopy] need help preparing intestinal villi for SEM and morphometry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

One of our investigators is working on knockout mice whose intestinal villi
are longer than those seen in wild type mice. She wants to show these by
SEM and hopefully also measure the average height of the villi. The
problem is how to prepare the intestine so that some of the villi are
standing up and how to obtain preparations that will allow measurement of
the villi.

I welcome any suggestions or references if you know of some older references.

Thanks,

Cora Bucana


==============================Original Headers==============================
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6, 19 -- Date: Fri, 14 Oct 2005 16:46:33 -0500
6, 19 -- To: Microscopy-at-microscopy.com
6, 19 -- From: "Corazon D. Bucana" {bucana-at-audumla.mdacc.tmc.edu}
6, 19 -- Subject: need help preparing intestinal villi for SEM and morphometry
6, 19 -- Mime-Version: 1.0
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From: ard-at-ansto.gov.au
Date: Fri, 14 Oct 2005 18:08:54 -0500
Subject: [Microscopy] Re: This new platform....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Some of us are now receiving unsolicited private invitations from
yahoo to join this new forum. It seems that behind Shuyou lurks our
old friend Mr Sergey Ryazantsev complete with a considerable amount
of political baggage in the invitation message with regard to Nestor
and the current listserver. Therefore one needs to question the
motives behind such an initiative, its stability, and its likely
endurance into the future.

This list has been running very well almost since electricity was
invented, thanks to Nestor, who for all we know may well have even
invented electricity once. I'm staying with this platform, thanks.


==============================Original Headers==============================
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From: shashis_99-at-yahoo.com
Date: Fri, 14 Oct 2005 23:46:00 -0500
Subject: [Microscopy] Re: need help preparing intestinal villi for SEM and morphometry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Cora,
You could do TEM studies of it and measure the
villus length, that's what my colleague did for
starvation stress in mice, where the villus size
changes.
shashi
CCMB
Hyderabad INDIA
--- bucana-at-audumla.mdacc.tmc.edu wrote:

}
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}
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}
} Hi All,
}
} One of our investigators is working on knockout mice
} whose intestinal villi
} are longer than those seen in wild type mice. She
} wants to show these by
} SEM and hopefully also measure the average height of
} the villi. The
} problem is how to prepare the intestine so that some
} of the villi are
} standing up and how to obtain preparations that will
} allow measurement of
} the villi.
}
} I welcome any suggestions or references if you know
} of some older references.
}
} Thanks,
}
} Cora Bucana
}
}
} ==============================Original
} Headers==============================
} 6, 19 -- From bucana-at-audumla.mdacc.tmc.edu Fri Oct
} 14 16:44:14 2005
} 6, 19 -- Received: from mdairnmail2.mdacc.tmc.edu
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} 6, 19 -- Date: Fri, 14 Oct 2005 16:46:33 -0500
} 6, 19 -- To: Microscopy-at-microscopy.com
} 6, 19 -- From: "Corazon D. Bucana"
} {bucana-at-audumla.mdacc.tmc.edu}
} 6, 19 -- Subject: need help preparing intestinal
} villi for SEM and morphometry
} 6, 19 -- Mime-Version: 1.0
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}


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

==============================Original Headers==============================
5, 20 -- From shashis_99-at-yahoo.com Fri Oct 14 23:45:59 2005
5, 20 -- Received: from web54615.mail.yahoo.com (web54615.mail.yahoo.com [206.190.49.185])
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5, 20 -- From: shashi singh {shashis_99-at-yahoo.com}
5, 20 -- Subject: Re: [Microscopy] need help preparing intestinal villi for SEM and morphometry
5, 20 -- To: bucana-at-audumla.mdacc.tmc.edu
5, 20 -- Cc: microscopy-at-microscopy.com
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From: gwe-at-ufl.edu
Date: Sat, 15 Oct 2005 09:15:51 -0500
Subject: [Microscopy] Biotech Director Job

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Director and professor

The mission of the Interdisciplinary Center for Biotechnology
Research (ICBR) is to support the growth of the life science research
program of the University of Florida and that of researchers
throughout the state, by making widely available the needed
facilities, technologies, training, and competent personnel. ICBR is
a service-oriented organization. The Director will manage the
personnel and core facilities in support of the research faculty
across all the colleges and departments of the University of Florida.

Interested candidates may contact me or go to this site and search
the academic positions.
http://jobs.ufl.edu/.


Gregory W. Erdos, Ph.D.
Assistant Director, Interdisciplinary Center for Biotechnology Research
Scientific Director, Electron Microscopy Core Lab
P.O. Box 118525
University of Florida
Gainesville, FL 32611
Phone: 352-392-1295
Fax: 352-846-0251
Email: gwe-at-ufl.edu


==============================Original Headers==============================
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6, 20 -- From: Greg Erdos {gwe-at-ufl.edu}
6, 20 -- Subject: Biotech Director Job
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From: winston.wiggins-at-cshs.org
Date: Sat, 15 Oct 2005 10:05:18 -0500
Subject: [Microscopy] viaWWW: Nikon Small World Photomicrography competition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (winston.wiggins-at-cshs.org) from http://www.microscopy.com/MLFormMail.html on Friday, October 14, 2005 at 11:06:29
---------------------------------------------------------------------------

Email: winston.wiggins-at-cshs.org
Name: Winston Wiggins

Organization: Cedars-Sinai Medical Center, Los Angeles, CA

Title-Subject: [Filtered] MListserver: Nikon Small World Photomicrography competition

Question: Since 1974, Small World has assembled panels of scientists and photo editors to honor the best and brightest in the field of photomicrography,... Small World unabashedly celebrates the aesthetic side of what the microscope sees and the camera records... a chance for scientists to be celebrated as artists. "Scientists can face a long, arduous, technical road to scientific discovery," Eric Flem, who runs the Small World show, says. "But along the way, these guys see some absolutely beautiful images."

~ excerpt from "Mama Don't Take My Microscope" at http://www.wired.com/news/culture/0,1284,69191,00.html.


---------------------------------------------------------------------------

==============================Original Headers==============================
8, 12 -- From zaluzec-at-microscopy.com Sat Oct 15 10:05:18 2005
8, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22])
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8, 12 -- To: microscopy-at-microscopy.com
8, 12 -- From: winston.wiggins-at-cshs.org (by way of MicroscopyListserver)
8, 12 -- Subject: viaWWW: Nikon Small World Photomicrography competition
8, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: s2kdude-at-pacbell.net
Date: Sat, 15 Oct 2005 13:20:48 -0500
Subject: [Microscopy] This new platform....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Competition is healthy and another source of help is
always welcome. We'd all be still using candles if it
wasnt for people like Edison coming up with
alternatives for us to chose.

--- ard-at-ansto.gov.au wrote:

}
}
}
}
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}
} Some of us are now receiving unsolicited private
} invitations from
} yahoo to join this new forum. It seems that behind
} Shuyou lurks our
} old friend Mr Sergey Ryazantsev complete with a
} considerable amount
} of political baggage in the invitation message with
} regard to Nestor
} and the current listserver. Therefore one needs to
} question the
} motives behind such an initiative, its stability,
} and its likely
} endurance into the future.
}
} This list has been running very well almost since
} electricity was
} invented, thanks to Nestor, who for all we know may
} well have even
} invented electricity once. I'm staying with this
} platform, thanks.
}
}
} ==============================Original
} Headers==============================
} 3, 24 -- From ard-at-ansto.gov.au Fri Oct 14 18:08:54
} 2005
} 3, 24 -- Received: from tachyon.gw.ansto.gov.au
} (tachyon.gw.ansto.gov.au [137.157.8.253])
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} {microscopy-at-microscopy.com}
} 3, 24 -- From: Arthur Day {ard-at-ansto.gov.au}
} 3, 24 -- Subject: Re: This new platform....
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} charset="us-ascii" ; format="flowed"
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} Headers==============================
}


==============================Original Headers==============================
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4, 19 -- Received: from web80727.mail.yahoo.com (web80727.mail.yahoo.com [66.163.170.92])
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4, 19 -- Subject: Re: [Microscopy] Re: This new platform....
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From: jfactor-at-ns.purchase.edu
Date: Sat, 15 Oct 2005 13:58:20 -0500
Subject: [Microscopy] Anew platform for microscopists to discuss

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A few additional points about the Microscopy Listserver.
* I would hate to have to go looking for info, or have to specify
topics of interest (it's all potentially interesting). Given my other
obligations, I would be much less likely to seek out the info, but I'm
happy to have it thrust in front of me. When I'm too busy, I simply
ignore the list message that day.
* I enjoy opening my email to all sorts of interesting questions,
answers, and discussion about various aspects of microscopy. It's all
right there. Sure, much of the discussion is not of interest to me at
the moment, but I learn lots by perusing the emails, even on topics I
would not have thought of as being interesting.
* The point has already been made that it's not such a large traffic
volume as to be unmanageable, and it's easy enough to hit the delete
button based on the message subject line. One could even set the junk
mail filter to automatically identify the list messages, then un-junk
the ones of interest, and auto-delete all the rest with one click.
* In other words, there are some real advantages to the email listserve
format. If there are real needs for additional capabilities, such as a
place to post image files that are referenced in the emails, perhaps
Nestor could take with up with MSA.
* Finally, I will add my "thank you!" to Nestor for maintaining such a
useful list as a public service, in spite of the occassional complaint
and with little thanks (I'm sure).
--Jan Factor

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College
State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------

==============================Original Headers==============================
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2, 23 -- Date: Sat, 15 Oct 2005 14:58:24 -0400
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2, 23 -- Subject: Re: [Microscopy] RE: Anew platform for microscopists to discuss
2, 23 -- microscopy online
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2, 23 -- Gecko/20040804 Netscape/7.2 (ax)
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From: opto-at-klughammer.de
Date: Sat, 15 Oct 2005 14:21:31 -0500
Subject: [Microscopy] Re: Anew platform for microscopists to discuss

Contents Retrieved from Microscopy Listserver Archives
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That's exactly how I see it.

Anna E. Schmaus
klughammer bio gmbh


jfactor-at-ns.purchase.edu schrieb:

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From: per.horstedt-at-medbio.umu.se
Date: Sat, 15 Oct 2005 18:53:34 -0500
Subject: [Microscopy] Re: This new platform....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Add my voice to this chorus. I agree that this format allows me the
extra pleasure of serendipity as well as choice.

Joel


Date sent: Sat, 15 Oct 2005 14:21:39 -0500
To: jbs-at-temple.edu
X-from: opto-at-klughammer.de
Send reply to: opto-at-klughammer.de

Sure, but how come you always light the candles when the electricity
fails.......


Cheers,
Per

Per Horstedt
Inst of medical biosciences Pathology
Dept of electron microscopy
University of Umea
S-90185 Umea
Sweden











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From: loz_jay01-at-hotmail.com
Date: Sun, 16 Oct 2005 09:27:53 -0500
Subject: [Microscopy] AskAMicroscopist: procedure scientists use to look at

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (loz_jay01-at-hotmail.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, October 16, 2005 at 06:59:58
---------------------------------------------------------------------------

Email: loz_jay01-at-hotmail.com
Name: laura

Organization: richard lander school

Education: 9-12th Grade High School

Location: truro,cornwall

Question: please could you tell me what procedure scientists use to look at cancer cells under the microscope

---------------------------------------------------------------------------

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7, 12 -- Subject: AskAMicroscopist: procedure scientists use to look at
7, 12 -- Content-Type: text/plain; charset="us-ascii"
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From: michael-at-shaffer.net
Date: Sun, 16 Oct 2005 12:31:14 -0500
Subject: [Microscopy] Image analysis - separating touching particles

Contents Retrieved from Microscopy Listserver Archives
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I am using a particular software for determining particle (and included
grains) identification. For separating touching particles the software
offers parameterization of 3 criteria: 'F', for control with regard to
features across the surface (e.g., cracks); 'T', for controlling particles
touching at a point; and 'E', for some control of particles touching along
longer joins. What makes the parameters a bit difficult to experiment with
is the number of permutations ... That is, the are 3 parameters for 'F' and
3 for 'E', for 7 total.

Does anyone recognize these parameters, such that they may provide me with a
reference for understanding parameter values and how they may affect one
another?

TIA ... michael shaffer :o)
Memorial University
St. John's Newfoundland


==============================Original Headers==============================
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From: mardre-at-mardre.com
Date: Mon, 17 Oct 2005 07:00:30 -0500
Subject: [Microscopy] Balzers 400D Freeze Fracture available

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Cora

can you just clarify whether it is the villi you are trying to measure
or the microvilli, because villi would be better done by some form of
light microscopy.

Thanks
Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
University of Sunderland
Tyne & Wear
SR1 3SD
UK

e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: shashis_99-at-yahoo.com

Dear Ursala

I had a look at phpScheduleIt previously but it is Unix based and apparently
needs an Apache web server ('Is that a helicopter dear?' - we're out of them
at the moment anyway).

I did think of doing something clever with Access, but ultimately we do fine
with separate A4 sheets, produced in word, one page per week, one month in
advance. The sheets are located outside the microscope rooms in a
wall-mounted clear plastic A4 display folders (with a pencil and a rubber).
It works better than a diary at least, and 'this week' only is 'on view'. It
also means you don't need a network linked laptop to see if the microscope
is free, and the 'hard copies' are easy to read. However if anyone has any
facility management software that's virtually free (and not £500 like most
on the web), I would be interested (possibly).

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


----- Original Message -----
X-from: {U.J.Potter-at-ns.microscopy.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Friday, October 14, 2005 1:48 PM

Available - complete Balzers 400D Freeze Fracture System with:

- electron beam guns EK552 for Pt-C and C,
- quartz crystal thin film monitor QSD-201D
- freeze etching unit control BMS101,
- HT unit control EVM052,
- pumping unit control DPA101,
- power distributor BNV201
- rotary pump (Pfeiffer DUO030A)
- liq. nitrogen supply FET003
- threefold specimen table for cleaving
- double replica specimen table for simultaneous breaking up of 3 sandwich carrier


Also included are
- extra HT unit control EVM052,
- extra freeze etching unit control GA-1,
- extra quartz crystal thin film monitor QSG101


Must move immediately for best offer.

Have a view of the device at http://www.mardre.com/homepage/mic/tem/equipment/baf400.html




*******************************************
Dr. Markus Drechsler
Electronmicroscopy
SFB481/BIMF/BZKG
University of Bayreuth, NW2
Universitaetsstr. 30
D-95440 Bayreuth
Tel: +49 (0)921 55-3188
Fax: +49 (0)921 55-3116

e-mail:
Markus.Drechsler-at-uni-bayreuth.de
mardre-at-mardre.com

http://www.uni-bayreuth.de/departments/mcii
http://www.mardre.com

*******************************************


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From: oxfordmaterials-at-hotmail.com
Date: Mon, 17 Oct 2005 07:57:56 -0500
Subject: [Microscopy] AskAMicroscopist: undergraduate Team Design Project for SEM

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (oxfordmaterials-at-hotmail.com) from
http://microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
Monday, October 17, 2005 at 05:39:54
---------------------------------------------------------------------------

Email: oxfordmaterials-at-hotmail.com
Name: Mike Dowling

Organization: Department of Materials, Oxford University

Education: Undergraduate College

Location: Oxford, UK

Question: I am currently heading a third-year undergraduate Team
Design Project at the Materials Department of Oxford University; our
aim being to
design a hypothetical SEM stage and environmental cell for in-situ CVD
synthesis and
observation of carbon nanotubes.

We would intend to grow the tubes in a low pressure (approx 1.5 Torr)
methane
environment, between 700 and 1100C. This hypothetical design currently
includes a
water-cooled heating stage equipped with screens to contain thermally
emitted electrons, which would hopefully also contain the gases
sufficiently, and negate the need for an expensive environmental cell unit.

We believe that this technology would be useful for studying nanotubes, but
could also be adapted to form a stage that would fit inside any existing
SEM for a variety of different high temperature dynamic reactions.

We would be
very grateful to recieve any help, ideas or advice that you may have at
oxfordmaterials-at-hotmail.com.

Thank you very much for your time,

Mike Dowling et al.
Department of Materials,
Oxford University.


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From: sksears-at-eps.mcgill.ca
Date: Mon, 17 Oct 2005 09:12:30 -0500
Subject: [Microscopy] Re: online booking software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A very good online scheduling system developed and maintained by the Complex Carbohydrate Research Center at The University of Georgia is offered free to users. The URL is http://faces.ccrc.uga.edu/. You can click on the 'Join Us' or 'FAQs' for more information.

Regards,

S. Kelly Sears



keith.morris-at-ucl.ac.uk wrote:

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From: a.chuvilin-at-microscopist.ru
Date: Mon, 17 Oct 2005 09:27:46 -0500
Subject: [Microscopy] TEM Postdoc positions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Two postdoctoral research associate positions are now opened is the group of
material science within the Center of Electron Microscopy of Ulm University
(Germany).

Positions are related to research projects devoted to the study of magnetic
nanomaterials and nanoporous oxides.
The main research instrument for these studies will be Titan 80-300 (HAADF
STEM detector, imaging Cs-corrector, Tridiem GIF, biprizm and Lorenz lens),
which is currently under installation in Ulm uni. There is a number of other
instruments in the Center, including CM20 TEM with heating and cooling
holders and Hitachi S5200 high resolution SEM.

Deadline for applications is 21st of November 2005.
Details can be found at http://www.uni-ulm.de/elektronenmikroskopie/mattem
following the link "Vacancies".

_________________
Andrey Chuvilin, PhD
University Ulm
Zentrale Einrichtung Elektronenmikroskopie
Materialwissenschaftliche Elektronenmikroskopie
Albert Einstein Allee 11
D-89069 ULM
Tel.: ++49-731-50-22941
Fax.: ++49-731-50-22958
Email: andrey.chuvilin-at-uni-ulm.de
http://www.uni-ulm.de/elektronenmikroskopie/mattem/


==============================Original Headers==============================
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From: ineke.joosten-at-icn.nl
Date: Mon, 17 Oct 2005 10:31:59 -0500
Subject: [Microscopy] Betr.: viaWWW: JEOL Cross section polisher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






The AMOLF FOM Institute for Atomic and Moleculair physics (Amsterdam, The
Netherlands) has purchased one only recently. You could contact Jaap Boon
for more information J.Boon-at-amolf.nl. However, the AMOLF is not a service
lab!





Ineke Joosten

onderzoeker afdeling Onderzoek/researcher department of Research
Instituut Collectie Nederland/Netherlands Institute for Cultural Heritage
Postbus 76709, 1070 KA Amsterdam/P.O. Box 76709, 1070 KA Amsterdam, The
Netherlands
T +31 (0)20 305 46 88/728
F +31 (0)20 305 47 00
E ineke.joosten-at-icn.nl

Het ICN is onderdeel van het Ministerie van OCW/ICN is part of the Ministry
of Education, Culture and Science
Zie voor meer informatie www.icn.nl/For more information see www. icn.nl
Aan deze email kunnen geen rechten worden ontleend/No rights may be derived
from this e-mail


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From: eggert-at-mikroanalytik.de
Date: Mon, 17 Oct 2005 12:25:18 -0500
Subject: [Microscopy] microanalysis service labs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi listers,

this is an information for those members who are a part of a public /
semi-public microanalysis service lab (electron microscope and/or x-ray
spectrometer equipment). If there is the wish to put the service offer
publicly, then you will find a new site to post your message without any
fees:


http://microanalysis.mikroanalytik.de/service%20microanalysis%20e.phtml

The site has the aim to bring people with microanalytical problems
together with them, who have the equipment and the possibility to solve
the analytic task.

Best regards

F.Eggert

============================
www.microanalyst.net
============================


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From: cammer-at-aecom.yu.edu
Date: Mon, 17 Oct 2005 14:14:07 -0500
Subject: [Microscopy] Accu-Scope 3016?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One of my colleagues is looking for an inexpensive fluorescent scope for
screening cells.

If you have any personal experience with the Accu-Scope 3016 or another
model (preferably with infinity corrected optics according to the brochure
at http://www.microscopestore.com/info/3015.pdf ) and you are not a vendor
of the microscope, we would greatly appreciate your feedback.

Thanks!

____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/
**This electronic transmission contains information that is privileged.**


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From: GBurgess-at-exchange.hsc.mb.ca
Date: Mon, 17 Oct 2005 15:57:09 -0500
Subject: [Microscopy] Neutralizing Glutaraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would be interested in what people use to neutralize their glutaraldehyde
for hazardous waste removal. This would be 2.5% buffered glut. as used to
fix human biopsy tissue.



Garry Burgess
Charge Technologist
Health Sciences Centre
Winnipeg

This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

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From: jeremiah.tyler-at-us.army.mil
Date: Mon, 17 Oct 2005 17:47:33 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: pursuing a degree in criminal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jeremiah.tyler-at-us.army.mil) from
http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Monday, October 17, 2005 at 16:16:22
---------------------------------------------------------------------------

Email: jeremiah.tyler-at-us.army.mil
Name: Jeremiah Tyler

Organization: Central Texas College

Education: Undergraduate College

Location: Panama City Beach, FL

Question: I am pursuing a degree in criminal investigation and as an
assignment I was to research the MSA website and submit my findings
to my fellow students in a discussion board. I understand microscopy
is a way of looking at items that are smaller than the eye can see
and to get a grasp on the application of microscopy in regards to CSI
is difficult for me since I am a visual person. What would be some
ways an investigator could utilize the services of a criminal
investigator?

---------------------------------------------------------------------------

==============================Original Headers==============================
7, 13 -- From zaluzec-at-microscopy.com Mon Oct 17 17:47:33 2005
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From: James.Roberts-at-ventura.org
Date: Mon, 17 Oct 2005 19:00:10 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: pursuing a degree

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jeremiah,

I'm not sure of the exact question you are asking. But if you are
asking how microscopy is used in the analysis of evidence, which is what
I think you are trying to get at, here are a few examples. Our trace
unit uses microscopy in most of it's analysis types. They use stereo
microscopes extensively just to characterize the evidence. Phase
contrast, polarized light and fluorescence microscopy is used for
different types of evidence. An example of this is that fibers would be
examined using a polarized light microscope to determine if it is
natural or synthetic. They use a comparison microscope to compare the
characteristics of a known and unknown fiber. The Scanning Electron
Microscope/ Energy dispersive spectrometer (SEM/EDS) is used for both
visual and elemental analysis of many evidence types, such as analysis
of GSR from hands or to characterize the trace materials that prove
ricochet when found embedded in the side of a bullet. Our Forensic
Biology unit uses microscopes to exam for the presence of sperm in rape
cases. We might use a video microscope unit to examine an area on a car
for paint transfer of to help do examination and measurements in blood
pattern analysis cases at a crime scene, it allows us to capture good
documentation of the evidence in a nice portable unit (we do digital
photos and film through many of our other scopes). Our drug chemistry
unit uses microscopes to conduct crystal tests on some types of drugs,
as one of several tests run. The unit I work in if Firearm and Toolmark
analysis. We use stereo microscopes to examine bullets and cartridge
cases for class characteristics. We use that same scope to characterize
a defect in a garment as a bullet hole and to look for gun powder
particle around the bullet hole as part of a range determination. After
test firing a firearm we use a comparison microscope to compare the
known bullet or cartridge case to the unknown (bullet/cartridge case
form the scene) for both class and individual characteristics. We might
be the one examining the bullet for proof of ricochet in the SEM.
These are just a few examples of the many, many uses microscopes are put
to in a modern Forensic Science lab. I hope this gives you an idea of
what we do with microscopes related to physical evidence, if you have
other questions contact me off the list.

Jim

James L. Roberts
Firearm & Toolmark Examiner
Ventura Co. Sheriff's Lab
800 S. Victoria Ave.
Ventura, CA. 93009

(805) 477-1947

James.Roberts-at-ventura.org



} } } {jeremiah.tyler-at-us.army.mil} 10/17/2005 3:50:50 PM } } }



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America

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jeremiah.tyler-at-us.army.mil) from
http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html

on Monday, October 17, 2005 at 16:16:22
---------------------------------------------------------------------------

Email: jeremiah.tyler-at-us.army.mil
Name: Jeremiah Tyler

Organization: Central Texas College

Education: Undergraduate College

Location: Panama City Beach, FL

Question: I am pursuing a degree in criminal investigation and as an
assignment I was to research the MSA website and submit my findings
to my fellow students in a discussion board. I understand microscopy
is a way of looking at items that are smaller than the eye can see
and to get a grasp on the application of microscopy in regards to CSI
is difficult for me since I am a visual person. What would be some
ways an investigator could utilize the services of a criminal
investigator?

---------------------------------------------------------------------------

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From: syli-at-northwestern.edu
Date: Mon, 17 Oct 2005 20:48:45 -0500
Subject: [Microscopy] re: viaWWW: online booking software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

FYI,

Here is a software I designed for Northwestern University. It is ASP-based, like to forum I am
presenting the other day here (maybe I'd better not mention this: ).

Have a look: http://www.nuance.northwestern.edu/fom

Shuyou
_____________________________
Shu-You Li, Ph.D.
Electron Microscopist, EPIC
NUANCE
Northwestern University
2220 Campus Drive, 1161 Cook Hall (#2036 for mail)
Evanston, IL 60208-3108, USA
Ph: (847) 491-6723(O), (847) 491-7806(L), Fax: (847) 467-6573
Email: syli-at-northwestern.edu; shuyouli-at-gmail.com
http://www.nuance.northwestern.edu/EPIChttp://www.shuyouli.com



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From: W.Muss-at-salk.at
Date: Tue, 18 Oct 2005 04:11:28 -0500
Subject: [Microscopy] Re: Neutralizing Glutaraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning,
dear Garry,

You ask for deactivation of used } glutaraldehyde { solution.....

there are methods which work at least in a "microcosmic-microchemical"
environment (say cells, or fixation solutions used in tissue preparation
for microscopy....): they all use either } glycine { solution, also
} Na-borohydride { solutions can be used, and another alternative would be
} ammonium-chloride { (NH4Cl) (hydrous or in the respective buffer) which
is/has been used in immunohistochemistry (for a long while) for blocking
free aldehyde groups (resting from prior fixation process within the tissue
blocks).

For such a purpose, ROTH J et al in the 1980ies or 1990ies stated 50 mM
hydrous solution of Ammoniumchloride (30 min) would be sufficient to block
all free aldehyde groups in tissue blocks (the deactivation process takes -
merely seen chemically - just a few seconds, but to be on the safe side
for the deactivation of free aldehyde groups not bound to tissular elements
in tissue blocks they recommended incubation of tissues for at least 30
mins at room temperature).

Concerning the concentration of such deactivating solutions for higher
concentrated fixative solutions (say 2.5-4% GA) : perhaps / for sure
excess in concentration used routinely for "microchemical" reaction are
appropriate } usually { to be on the safe side.....

Since I have read some week ago that some commercially available
deactivators of formaldehyde solutions (available in the USA, perhaps
Canada too) like {Formalex} etc. are not suited for a deactivation of
Glutaraldehyde, I use another deactivation method I was told many years
ago,

namley highly alkalizing the (diluted) hydrous form- as well as
glutar-aldehyde solution(s) by adding enough 10 N NaoH solution (which is
also produced as a "waste" solution....after staining ultrathin sections
you would have to } dispose of { sodiumhydroxide pellets.....I collect them
into a bottle with water.....get nearly 5-10 N solution...and use it for
such deactivation processes...)
.....Unfortunately, I do not remember the source of that information as
well as the efficiacy of such a deactivation.....

Also there have been reported other chemical reagents used for blocking
aldehyde groups, namely sodium bisulfite as well as hydroxylamine (reagents
I don't have experience with).


But now I tried GOOGLE search for "aldehyde deactivation" and came over
some websites offering commercially available deactivation solutions, like:
PREMIDEX (C) ADS2: deactivates GA-solutions up to 4% concentration: comp.
http://www.metrex.com/products/unitedStates/premidexADS2/index.cfm
or: found at:
http://www.discount-office-supplies-resources.org/catalog1/Banta-Tissue-
Drape.html

Shilog Product Catalog Sorted by Category
(http://www.discount-office-supplies-resources.org/main.php?tracker=www.
shilog.com/Web_Site_Store/catagory.html )
Shilog Medical Supply. P.O. Box 1887. 3551 Mt. Moriah Road. McAlester. Ok,
74502. Toll Free: 1-800-574-4868. McAlester: 1-918-423-4447. Fax:
918-423-5569. E-Mail Us. Aldehyde Deactivation System (perhaps this also
points to PREMIDEX ADS2).

Also you can use the search phrase "aldehyde blocking" or "Formalin
deactivation"...you will find again some references....


Regards

Wolfgang Muss
Personal Communication Data:
OR Dr. Wolfgang Muss Member of MSA, etc, FRMS
Head of EM-Lab
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively

Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
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Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
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----------
Von: GBurgess-at-exchange.hsc.mb.ca[SMTP:GBurgess-at-exchange.hsc.mb.ca]
Antwort an: GBurgess-at-exchange.hsc.mb.ca
Gesendet: Montag, 17. Oktober 2005 23:02
An: W.Muss-at-salk.at
Betreff: [Microscopy] Neutralizing Glutaraldehyde




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I would be interested in what people use to neutralize their glutaraldehyde
for hazardous waste removal. This would be 2.5% buffered glut. as used to
fix human biopsy tissue.



Garry Burgess
Charge Technologist
Health Sciences Centre
Winnipeg

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From: ptomic-at-ciclonsemi.com
Date: Tue, 18 Oct 2005 07:45:37 -0500
Subject: [Microscopy] [Filtered] AskAMicroscopist: pursuing a degree in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jeremiah,

When going beyond the apparently visual aspects of crime scenes, the trace
evidence is where the microscopist would reside. For example, and these are
just some items that are employed in crime scene processing, this list is
not meant to be all inclusive.

1. Metals identification, e.g. plating from a bumper in a hit and run auto
accident - metal fragments from knives
2. Identification of paints
3. Gunshot residue using SEM-EDX
4. Hair and fiber identification
5. Tool mark identification [pipe bombs for example and product tampering]

Along with the above there are other disciplines such as entomology that can
analyze the type and progression of insects during the decomposition of the
body thus pinning time of death and in some cases place of death. I should
not leave out the most important which is DNA identification.

There is plenty of literature out there to help you.

A detective friend of mine told me that one can never enter and leave a
crime scene without leaving something behind or exchanging something. Just
think, you shed several million skin particles a day. One is bound to leave
a few at the scene among other things.

Good luck in your studies.

Peter Tomic
Ciclon Semiconductor Device Corp.
Bethlehem, PA



-----Original Message-----
X-from: jeremiah.tyler-at-us.army.mil [mailto:jeremiah.tyler-at-us.army.mil]
Sent: Monday, October 17, 2005 6:52 PM
To: ptomic-at-ciclonsemi.com

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (jeremiah.tyler-at-us.army.mil) from
http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Monday, October 17, 2005 at 16:16:22
---------------------------------------------------------------------------

Email: jeremiah.tyler-at-us.army.mil
Name: Jeremiah Tyler

Organization: Central Texas College

Education: Undergraduate College

Location: Panama City Beach, FL

Question: I am pursuing a degree in criminal investigation and as an
assignment I was to research the MSA website and submit my findings
to my fellow students in a discussion board. I understand microscopy
is a way of looking at items that are smaller than the eye can see
and to get a grasp on the application of microscopy in regards to CSI
is difficult for me since I am a visual person. What would be some
ways an investigator could utilize the services of a criminal
investigator?

---------------------------------------------------------------------------

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From: William.H.Roberts-at-USA.dupont.com
Date: Tue, 18 Oct 2005 09:52:46 -0500
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: pursuing a degree in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Jeremiah,

I was a forensic scientist for about nine years (back in the seventies) and
was the supervisor of the trace evidence section of the lab. I used a
variety of techniques including optical and scanning electron microscopy,
x-ray diffraction, gas chromatography/mass spec, and x-ray fluorescence in
addition to a number of physical test procedures. The key to working with
a trace evidence lab is maintaining flexibility. Present your evidence and
allow the analyst the opportunity to review it and suggest the appropriate
testing protocols. While there are some standard approaches to the
analysis of common evidence types such as paint, it pays to keep an open
mind with regards to what exactly constitutes evidence and how that
evidence might best be analyzed. The reason for this is that evidence
plays two roles in the investigation/prosecution of a case. The first use
of evidence is during the investigation phase of a case where the evidence
often plays a part in the identification of a suspect. Here the analyst
can often, though not always, be of significant help to the investigator by
revealing information gleaned from the evidence such as hair color, or the
color of a hit and run vehicle (maybe even the make and model in some
situations), shoe size and brand (and by extension an idea of the height
and weight of the suspect). The reason for keeping an open mind is that
some types of tests performed and the information which results may be
useful in identifying a suspect, but not in the second or prosecution phase
of a case. Here is where cases can be made or lost depending on the care
taken by the team of crime scene investigators and forensic scientists.
There are strict rules of evidence which are designed to protect the
innocent from either careless or willful tampering or contamination of the
evidence. When one is concerned with particles of matter which are
invisible until examined microscopically, there is a great risk of loss,
contamination, co-mingling, or misinterpretation of that evidence. Also,
the tests which are performed for the purpose of generating evidence to
which testimony will be given in court must meet certain standards
regarding admissability. Of course, the credentials of the analyst are
paramount, but the tests must be generally accepted in the scientific
community. Here, the use of the microscope is invaluable in that
microscopical techniques for hair and fiber, paint, soil, gunshot residue,
glass, tool marks, and firearm identification are well established and
generally accepted in court when performed by competent and ethical
analysts.

If you need more specific information on the nature of the evidence
examined or the types of tests which can be performed you can contact me
directlly at william.h.roberts-at-usa.dupont.com

Regards, Bill

jeremiah.tyler-at-us.army.mil wrote on 10/17/2005 06:48:19 PM:

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} Below is the result of your feedback form (NJZFM-ultra-55). It was
} submitted by (jeremiah.tyler-at-us.army.mil) from
} http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
} on Monday, October 17, 2005 at 16:16:22
}
---------------------------------------------------------------------------
}
} Email: jeremiah.tyler-at-us.army.mil
} Name: Jeremiah Tyler
}
} Organization: Central Texas College
}
} Education: Undergraduate College
}
} Location: Panama City Beach, FL
}
} Question: I am pursuing a degree in criminal investigation and as an
} assignment I was to research the MSA website and submit my findings
} to my fellow students in a discussion board. I understand microscopy
} is a way of looking at items that are smaller than the eye can see
} and to get a grasp on the application of microscopy in regards to CSI
} is difficult for me since I am a visual person. What would be some
} ways an investigator could utilize the services of a criminal
} investigator?
}
}
---------------------------------------------------------------------------
}
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From: herro001-at-umn.edu
Date: Tue, 18 Oct 2005 10:29:47 -0500
Subject: [Microscopy] Image storage?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have just started doing time lapse imaging of cells in culture.
As expected, the amount of data being produced is large. I expect to
produce about 200 GB every couple weeks (if I am careful). The files
are usually too large to be transfered to DVD (last night's
acquisitions totaled more than 30GB).

I am considering getting an external drive case (USB2 and/or 1394
(firewire)) and every couple of weeks swapping a new drive into this
case. The full drives would then go on a shelf for possible later
data retrieval. Right now 200 GB internal drives can be had for about
$70, so filling a couple dozen of these drives in a year would come
to about $2000.

What do the rest of you do to store this sort of data?


---

Michael J. Herron, U of MN, Dept. of Entomology
herro001-at-umn.edu
612-624-3688 (office) 612-625-5299 (FAX)



==============================Original Headers==============================
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From: Elliott-at-arizona.edu
Date: Tue, 18 Oct 2005 10:59:47 -0500
Subject: [Microscopy] Re: Image storage?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would not put too much faith in anything that spins. Drives fail.
If the data matters, I would think about something else.
Tape is a much better option. It is more stable and each tape is
cheeper. You may not pay for the increased cost of the tape drive with
the savings of tape vs. HD, but the data will be more secure.
I have set up an off site tape backup of my labs data (while I am at it
I also backup some of the departmental data). Each night the new data
is sucked off of the 1.5TB drive array that sits under my desk and is
put on tape. This involves very little work for me, and great data
safety. Even if the lab/building/university becomes a smoking whole in
the ground, the data is off site and safe.
You can always set up better systems with more money, mine is not the
best, but it did not cost much and I sleep better at night.
David


On Oct 18, 2005, at 8:32 AM, herro001-at-umn.edu wrote:

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}
} We have just started doing time lapse imaging of cells in culture.
} As expected, the amount of data being produced is large. I expect to
} produce about 200 GB every couple weeks (if I am careful). The files
} are usually too large to be transfered to DVD (last night's
} acquisitions totaled more than 30GB).
}
} I am considering getting an external drive case (USB2 and/or 1394
} (firewire)) and every couple of weeks swapping a new drive into this
} case. The full drives would then go on a shelf for possible later
} data retrieval. Right now 200 GB internal drives can be had for about
} $70, so filling a couple dozen of these drives in a year would come
} to about $2000.
}
} What do the rest of you do to store this sort of data?
}
}
} ---
}
} Michael J. Herron, U of MN, Dept. of Entomology
} herro001-at-umn.edu
} 612-624-3688 (office) 612-625-5299 (FAX)
}
}
}
} ==============================Original
} Headers==============================
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==============================Original Headers==============================
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From: tivol-at-caltech.edu
Date: Tue, 18 Oct 2005 11:40:51 -0500
Subject: [Microscopy] Job Opportunity at The Aeropspace Corp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,
A colleague asked me to post this job opening.
 

The Aerospace Corporation in El Segundo, CA has an opening for a Member
of the Technical Staff in the Microelectronics Technology Department.

JOB DUTIES:

Conduct basic and applied research using high resolution transmission
electron microscopy (HR TEM), electron energy loss spectroscopy (EELS),
electron diffraction, energy filtered imaging, electron tomography and
other advanced TEM techniques. The successful candidate will be
expected to develop innovative research programs involving the
application of the state-of-the-art TEM techniques to materials
research and microelectronic device analyses. Presentation of results
in scientific meetings and publication in refereed journals is
expected. The candidate will also be required to provide technical
support to various US Air Force space programs.
QUALIFICATIONS:

Ph.D. in physics, physical chemistry, materials science, electrical
engineering, or related technical discipline required. Experience in
characterizing materials and structures at the nanoscale is required.
A strong background in solid state and device physics is essential.
Ability to formulate and carry out independent research and targeted
investigations is a crucial requirement. Expertise required in one or
more of the following micro-analytical techniques: focused ion beam
techniques, high-resolution transmission electron microscopy, electron
energy-loss spectroscopy, or field-emission scanning electron
microscopy. Ph.D. and/or Postdoctoral research experience shall
include designing and conducting experimental studies, and performing
data analyses. Additional background or expertise in mathematical
analysis, device modeling and computer-based simulation is desirable.
Candidates must have good oral communication and technical writing
skills and the ability to work effectively as part of a team. US
citizenship required.


Location: The Aerospace Corporation is located in El Segundo, CA, a
beach community just outside of Los Angeles. Aerospace is 2 miles from
Los Angeles International Airport. Employees at Aerospace experience
the outstanding southern California climate, have access to year-round
beach activities and ski resorts open in the local mountains during a
substantial part of the year, as well as sporting and cultural
activities in the greater Los Angeles area.

For further information, please contact Martin S. Leung or Steven C.
Moss at

Martin S. Leung, Ph.D.
The Aerospace Corporation
P.O. Box 92957, MS/M2-244
Los Angeles, CA 90009-2957
Tel 310-336-7125
Email: martin.s.leung-at-aero.org

Steven C. Moss, Ph.D.
The Aerospace Corporation
P.O. Box 92957, MS/M2-244
Los Angeles, CA 90009-2957
Tel 310-336-9216
Email: steven.c.moss-at-aero.org


Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu



==============================Original Headers==============================
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From: gary-at-gaugler.com
Date: Tue, 18 Oct 2005 11:55:08 -0500
Subject: [Microscopy] Re: Image storage?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

USB and Firewire drive controllers are somewhat flakey.
So are the drives. I had a Maxtor that died about 6
months after new. The current nod is to Western Digital.

The quality of hard drives varies every year from maker
to maker. One year one maker is tops, the next, down
at the bottom. So, choose wisely.

The other problem is that drives don't like being
turned on and off. For highest reliability, they should
be left on all the time until tracks or sectors start
to fail. When this happens, in a RAID system, the failing
drive is replaced and the good data is mirrored back over.
A single drive is a single point of failure.

A better option is to use tape. Best (IMO) is Ultrium 2 or 3.
Next option is DLT. Drives are pricey. Media costs about
$85 each. So once you get past the cost of the drive, the
media is about the same as the hard drive. If you are really
paranoid like me, I write two backup tape sets and store one
off-site. I use NovaNET backup software and it will back up
all PCs on my LAN. There is about 900GB of data stored.

gary g.




At 08:31 AM 10/18/2005, you wrote:


} We have just started doing time lapse imaging of cells in culture.
} As expected, the amount of data being produced is large. I expect to
} produce about 200 GB every couple weeks (if I am careful). The files
} are usually too large to be transfered to DVD (last night's
} acquisitions totaled more than 30GB).
}
} I am considering getting an external drive case (USB2 and/or 1394
} (firewire)) and every couple of weeks swapping a new drive into this
} case. The full drives would then go on a shelf for possible later
} data retrieval. Right now 200 GB internal drives can be had for about
} $70, so filling a couple dozen of these drives in a year would come
} to about $2000.
}
} What do the rest of you do to store this sort of data?
}
}
} ---
}
} Michael J. Herron, U of MN, Dept. of Entomology
} herro001-at-umn.edu
} 612-624-3688 (office) 612-625-5299 (FAX)


==============================Original Headers==============================
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From: dkinast-at-hitschfel.com
Date: Tue, 18 Oct 2005 14:08:00 -0500
Subject: [Microscopy] LM Job Opportunities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Fellow Listers,


Hitschfel Instruments, Incorporated is a consultative sales company,
providing microscope based imaging solutions to the biomedical research,
bio-industrial, clinical and educational communities throughout the states
of Missouri, Nebraska, Kansas, Oklahoma and Arkansas/Memphis plus central &
southern Illinois.

We are seeking two exceptional and highly motivated individuals to work with
us toward our mutual goals of success through customer satisfaction. The
successful candidates for these Technical Sales positions will represent the
Olympus microscope product line, as well as a variety of digital imaging
hardware/software systems and other ancillary products within either our
Kansas territory or our Arkansas/Memphis territory. These positions would
carry the responsibilities of all aspects of the sales process to include
initial contacts, consultative development of sales opportunities, product
demonstrations, equipment delivery/assembly/in-service and after sales
follow-up. You will also work in conjunction with our Imaging Technology
Specialist to assist in the process of selling the most complex Image
analysis and automated microscopy solutions.

The personal characteristics and qualifications that we are looking for are:

. A BS degree in the Life Sciences as a minimum
. Biomedical research lab experience highly preferred
. Microscopy and digital imaging skills highly preferred
. Excellent communication and interpersonal skills
. A high degree of self motivation and work ethic
. Computer skills, including software/hardware installation

Previous sales experience would also be preferred, but if you are the right
person for the job, we will teach you the selling skills that you will need
to be successful.

This is an excellent commissioned sales career opportunity! We offer a
competitive income potential, along with a liberal benefits package. If you
are thinking about an alternative career in science that will put your
knowledge of biomedical technology to use in the business world, please
forward your resume with a cover letter to me at: dkinast-at-hitschfel.com.


David L. Kinast
Sales Manager
Hitschfel Instruments, Inc.
2333 South Hanley Rd.
St. Louis, MO 63144
Phone:800/242-3501
Phone: 314/644-6660
Fax: 314/644-5877
dkinast-at-hitschfel.com
http://www.hitschfel.com
http://www.hitschfel.com/linecard.html
http://www.olympusmicroscopes.com


==============================Original Headers==============================
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13, 22 -- Cc: {ghitschfel-at-hitschfel.com}
13, 22 -- Subject: LM Job Opportunities
13, 22 -- Date: Tue, 18 Oct 2005 14:07:14 -0500
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From: keith.morris-at-ucl.ac.uk
Date: Wed, 19 Oct 2005 03:58:36 -0500
Subject: [Microscopy] Image storage?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Also have a look at hot swappable hard drive cages (see below).

X-from my personal experience of tape backup, not only is it very slow and
expensive, I have abandoned it due to reliability problems: the only two
times I really needed it the archived tapes were 'corrupted. I have since
given up with any form of file compression for the same reason.

In comparison we haven't had any hard drive fail on the 12 PC's we use for
imaging and they are all guaranteed for 5 years, and we have up to four hard
drives per PC - although upgrade the power supply [PSU] to 450w+ from the
paltry ones normally supplied. Being 'poor', I often use ebay for very cheap
processor upgrades to the max the motherboard can take and crucial.com for
more memory (although be warned Windows 98SE & ME OS can only handle 512Mb
max). I always avoid Fujitsu drives from experience, and I tend towards
Western Digital from habit, rather than performance, and as I'm used to
their website for utilities. External hard drives are also rather slow, even
with firewire and USB2, and I tend not to trust them. I only use them for
occasional transfer between PC's as they are much faster than the network.
The only problem we have is our older PC's have internal hard drive BIOS
limits e.g. 128Gb per hard drive, which can only be overcome by a tacky. I
tend to have S.M.A.R.T. on, which should help identify failing drives.

Hot swappable drive cages

The option I would have a look at is internal hot-swappable SATA hard drive
cages (or IDE depending on motherboards) as these are cheap and reliable per
Gb. I never use SCSI anymore as the cost of these is prohibitive per Gb and
they aren't even significantly faster anymore. You can be clever and use
raid etc.. but we don't, we just backup to a second media (normally optical
with data verification) as soon as the data is collected.

With hot swappable hard drive cages you simply pull out the hard drives and
replace them rather like floppy disks. Being hot swappable you can naturally
do this while the PC is running (they are designed for servers). But of
course you can do it while the system is off. e.g.
http://www.rackmount.com/Rackacc/HDC-300.htm

The OS remains on another internal drive.

That is a lot of data you are producing though, each of our OpenLabs or
confocal time-lapse systems produce far smaller total file sizes per week,
so DVD's and CD's are OK for us at the moment. User's here are responsible
for their own backup, although I provide more than adequate storage space
(300Gb+ per PC) and full back-up facilities for them - after all only they
know which images are important.

Regards

Keith




==============================Original Headers==============================
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From: Mike.Bode-at-soft-imaging.net
Date: Wed, 19 Oct 2005 10:11:26 -0500
Subject: [Microscopy] Image storage?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Well, it's all about how paranoid you want to be...

Here, we back up our data daily with an incremental backup, then do a full backup on a regular basis, and then we backup the backup to a different site. Just in case the building burns down....

Regarding disk drives and tapes: Perhaps you have been lucky, but the mean time between failures for hard disks is not infinite. It is not a quuestion of if, but rather when a hard disk will fail. And invariably they fail at the most inopportune moment. I had my hard disk crash on my laptop during M&M this year, so I had to do heart surgery on my laptop on the show floor and limp along for a week until I could get back to our network and get it back in full working condition. We use a RAID system here to store data, and we lose a disk once in a while. Because we are using RAID, it is not a big deal. Take out the old disk, plug in a new one, and have the system rebuild the information on the new disk.

If you use a tape for backup, it is a good idea to run a couple of tests before you put it away to make sure the tape is OK, and store it in a place where the data doesn't deteriorate (climate controlled, no strong magnetic fields).

Hot swappable drives are good, but not fail-safe, unless you use 2 copies. We use them here, too, and have not had any problems, but again, it's a normal disk drive and will fail eventually. By using multiple drives and rotating them, you can probably extend the mean time between failure to a timeframe that is long enough for you.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: keith.morris-at-ucl.ac.uk [mailto:keith.morris-at-ucl.ac.uk]
Sent: Wednesday, October 19, 2005 3:03 AM
To: Mike Bode

Also have a look at hot swappable hard drive cages (see below).

X-from my personal experience of tape backup, not only is it very slow and expensive, I have abandoned it due to reliability problems: the only two times I really needed it the archived tapes were 'corrupted. I have since given up with any form of file compression for the same reason.

In comparison we haven't had any hard drive fail on the 12 PC's we use for imaging and they are all guaranteed for 5 years, and we have up to four hard drives per PC - although upgrade the power supply [PSU] to 450w+ from the paltry ones normally supplied. Being 'poor', I often use ebay for very cheap processor upgrades to the max the motherboard can take and crucial.com for more memory (although be warned Windows 98SE & ME OS can only handle 512Mb max). I always avoid Fujitsu drives from experience, and I tend towards Western Digital from habit, rather than performance, and as I'm used to their website for utilities. External hard drives are also rather slow, even with firewire and USB2, and I tend not to trust them. I only use them for occasional transfer between PC's as they are much faster than the network.
The only problem we have is our older PC's have internal hard drive BIOS limits e.g. 128Gb per hard drive, which can only be overcome by a tacky. I tend to have S.M.A.R.T. on, which should help identify failing drives.

Hot swappable drive cages

The option I would have a look at is internal hot-swappable SATA hard drive cages (or IDE depending on motherboards) as these are cheap and reliable per Gb. I never use SCSI anymore as the cost of these is prohibitive per Gb and they aren't even significantly faster anymore. You can be clever and use raid etc.. but we don't, we just backup to a second media (normally optical with data verification) as soon as the data is collected.

With hot swappable hard drive cages you simply pull out the hard drives and replace them rather like floppy disks. Being hot swappable you can naturally do this while the PC is running (they are designed for servers). But of course you can do it while the system is off. e.g.
http://www.rackmount.com/Rackacc/HDC-300.htm

The OS remains on another internal drive.

That is a lot of data you are producing though, each of our OpenLabs or confocal time-lapse systems produce far smaller total file sizes per week, so DVD's and CD's are OK for us at the moment. User's here are responsible for their own backup, although I provide more than adequate storage space (300Gb+ per PC) and full back-up facilities for them - after all only they know which images are important.

Regards

Keith




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13, 28 -- From keith.morris-at-ucl.ac.uk Wed Oct 19 03:58:36 2005 13, 28 -- Received: from vscani-a.ucl.ac.uk (vscani-a.ucl.ac.uk [144.82.108.29])
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28, 24 -- From Mike.Bode-at-soft-imaging.net Wed Oct 19 10:11:26 2005
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From: john.mardinly-at-intel.com
Date: Wed, 19 Oct 2005 10:51:51 -0500
Subject: [Microscopy] Image storage?

Contents Retrieved from Microscopy Listserver Archives
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We are using a LaCie Biggest Disk array. It has four 250GB hard drives
in a raid 5 configuration (Raid 0 and 1 are options) netting 750GB. It
has a Firewire 800 and USB 2.0 interface so installation is trivial. The
only problem we have seen is that the Firewire is quite slow on our old
computer that also runs the Gatan camera and GIF. It is fast on USB 2.0
on another computer. Vendor support did not go to help debug the
computer, since the drive seemed to be working. There is more on:
http://www.lacie.com/products/
On this system, if one spindle fails, the data can be recovered since it
is redundant on the other spindles. Tape is theoretically good too, but
don't be overconfident that you can restore from a tape that has been
sitting on a shelf for a few years.

John Mardinly
Intel Corporation

The opinions of this author do not necessarily reflect the opinons of
Intel Corporation.

-----Original Message-----
X-from: herro001-at-umn.edu [mailto:herro001-at-umn.edu]
Sent: Tuesday, October 18, 2005 8:30 AM
To: Mardinly, John

We have just started doing time lapse imaging of cells in culture.
As expected, the amount of data being produced is large. I expect to
produce about 200 GB every couple weeks (if I am careful). The files
are usually too large to be transfered to DVD (last night's
acquisitions totaled more than 30GB).

I am considering getting an external drive case (USB2 and/or 1394
(firewire)) and every couple of weeks swapping a new drive into this
case. The full drives would then go on a shelf for possible later
data retrieval. Right now 200 GB internal drives can be had for about
$70, so filling a couple dozen of these drives in a year would come
to about $2000.

What do the rest of you do to store this sort of data?


---

Michael J. Herron, U of MN, Dept. of Entomology
herro001-at-umn.edu
612-624-3688 (office) 612-625-5299 (FAX)



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From: jquinn-at-www.matscieng.sunysb.edu
Date: Wed, 19 Oct 2005 11:24:15 -0500
Subject: [Microscopy] thread size on B&L SZ4

Contents Retrieved from Microscopy Listserver Archives
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Folks

Off hand, does anyone know the
thread size on a Bausch&Lomb (B&L)
StereoZoom 4 (SZ4) nose/objective?

Any vendors wanting to sell me
filters, doublers, etc.... are
welcome to contact me off-list.

It looks be be around 1-1/2"x30.

regards,

Jim





==============================Original Headers==============================
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From: richard.beanland-at-bookham.com
Date: Thu, 20 Oct 2005 10:58:52 -0500
Subject: [Microscopy] Micrion Service Manual needed

Contents Retrieved from Microscopy Listserver Archives
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Hi Listers,
does anyone have a copy of the Micrion focused ion beam microscope service manual, part number 800 000033, "Service Procedures 2000 Manual" which they would be willing to copy (or lend to me so I can copy and return)? I'm happy to pay any costs involved.
I have tried via FEI but they are unable to help me, so I figure the listserver is my best hope.

Many thanks

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


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From: emlabservices-at-cox.net
Date: Thu, 20 Oct 2005 15:26:17 -0500
Subject: [Microscopy] TEM -- JEOL JEM-100C Free

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

We have a JEOL Model JEM-100C TEM that is to be deinstalled next week. It
is presently located in south Texas. If there is any interest in acquiring
this instrument please contact me off line asap.

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St.
Topeka, Kansas 66617-1780
785.246.1232 voice
785.246.0168 fax
www.emlabservices.com



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From: Stacey.Andringa-at-uc.edu
Date: Thu, 20 Oct 2005 17:29:03 -0500
Subject: [Microscopy] viaWWW: digitizing tablet and mouse

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Stacey.Andringa-at-uc.edu) from http://www.microscopy.com/MLFormMail.html on Thursday, October 20, 2005 at 08:37:17
---------------------------------------------------------------------------

Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

Organization: University of Cincinnati

Title-Subject: [Filtered] MListserver:

Question: Does anyone have an old working digitizing tablet and mouse that needs a good home? Our old tablet reached "end of life" nine years ago, but just now died.
We are on a tight budget and have been looking for a used one.
Thanks in advance for any help.

Stacey Andringa

---------------------------------------------------------------------------

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From: mbisher-at-princeton.edu
Date: Thu, 20 Oct 2005 17:29:25 -0500
Subject: [Microscopy] viaWWW: staining information for the polymer particles

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mbisher-at-princeton.edu) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, October 20, 2005 at 08:54:15
---------------------------------------------------------------------------

Email: mbisher-at-princeton.edu
Name: Margaret Bisher

Organization: Princeton University

Title-Subject: [Filtered] MListserver:

Question: Here we are looking for staining information for the polymer particles we
would like to look at under TEM. The polymer particles are dispersed on the
TEM grid coated with holy carbon film. The particles are composed of Poly
(Ethylene glycol) PEG) + b-Poly(carprolactone)(PCL) (5k-7k). The average
diameter of particles is about 200nm. The diameter of the core of the
particles (PEG) is about 140nm, and the polymer brush(PCL)outside core is
about 30nm long.

---------------------------------------------------------------------------

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From: jae5-at-lehigh.edu
Date: Fri, 21 Oct 2005 07:43:57 -0500
Subject: [Microscopy] Vacancy for a post doc

Contents Retrieved from Microscopy Listserver Archives
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Dear Michael,

Yes external Hard drive cages do exist, but if you are going 'external' a
few 300Gb+ Lacie or Maxtor/WesternDigital Firewire/USB2 drives may be easier
and probably cheaper (and they are fairly 'hot swappable' as well by simply
unplugging the USB2/Firewire lead).

Have a look at the £230 LaCie 500GB Big Disk Extreme FW400/800 USB2 7200rpm
16MB cache or the £500 LaCie 1000GB Bigger Disk Extreme 7200rpm Fast
Firewire (seems to have been discountinued).

There is also the Lacie two Terabyte fully hot swappable F800 HD 'cartridge'
system, if you must, but then you are going over £1,500 for the total
convenience of hot-swappable drives (with the USB2 still plugged in). LaCie
offers a spare drives and drawers (just like the internal cages) for use
with the LaCie Biggest F800. You can immediately and easily hot-swap a
failed drive etc. These Lacie products are faster than most if not all other
USB/Firewire external units, and are highly regarded.

See it all at:
http://www.lacie.com/products/range.htm?id=10033

The large number of hard drives enclosed in the Lacie non 'hot-swappable' HD
units makes them trans-staggerable rather than portable though.

External Ultra-SCSI HD cages would also be ideal, fed via a SCSI out PCI
card, but the price of SCSI drives is totally prohibitive per Gb.

With regard to internal PC heat, our PC's have extra fans in cabinets
(although they aren't very effective), but being mostly fairly elderly the
CPU's don't generate the heat of latest PC's. Upgrading the power supply
(PSU) is a big improvement as these also have temperature control and
superior extraction fans (and most case's heat-flow are designed to have
heat exit this way). I regularly remove dust bunnies from inside the PC's as
well and the PC's are kept on benches rather than the floor to reduce dust
contamination (dust insulation causes temperature build-up). An air-jet plus
Hoover works fine (but avoid Hoovering electronics directly to prevent
static build up).

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk



----- Original Message -----
X-from: "Michael Herron" {herro001-at-umn.edu}
To: {keith.morris-at-ucl.ac.uk}
Sent: Wednesday, October 19, 2005 11:24 PM

Electron Backscattering Diffraction
in the
Scanning Electron Microscope

Immediate vacancy for a post doc.


My post doc is leaving me to go to a great job with General Electric, so
I have a year and a half of funding with no one to do the work. I am
looking for a post doc, starting immediately (or as soon as is
practical) and ending May 31, 2007.

This is a DOE-funded project entitled: Strain Measurement in Thin Films.
The main focus of the work is developing and extending the technique
of EBSD in the SEM. The work involves EBSD, energy-filtered EBSD, and
writing new procedures for analyzing data. A new SEM that will be
chosen to give optimum EBSD performance will be ordered before the end
of the year to supplement the already very strong set of instruments
available at Lehigh for this work. You can get some idea of what is
wanted from looking at previous work from this group:
Microscopy and Microanalysis 11 (2005) 341-353
Microscopy and Microanalysis 11 Supplement 2 (2005) 524-525

The ideal candidate will have prior experience of three kinds:
experimental electron microscopy, dynamical diffraction theory and
programming.

The next steps are a) to further the use of EBSD to measure strain and
to apply it to electromigration, b) to develop better spatial resolution
in EBSD through energy filtering and c) to write software to simulate
EBSD patterns.

Candidates interested in this post please contact me. If you have sent
me email enquiring about a job and have got a reply saying I have no
vacancies (which was true at the time), please feel free to send your
details again - I have deleted the information you sent previously.

Alwyn Eades

--
...........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


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From: familia_zepeda-at-yahoo.com
Date: Fri, 21 Oct 2005 08:02:05 -0500
Subject: [Microscopy] AskAMicroscopist:inventor of polorizing microscope

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (familia_zepeda-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, October 20, 2005 at 22:15:54
---------------------------------------------------------------------------

Email: familia_zepeda-at-yahoo.com
Name: Irene Zepeda

Organization: Canyon Springs High school

Education: 9-12th Grade High School

Location: North Las Vegas, Nevada 89030

Question: I am doing a research project on William Nichol who invented the polorizing microscope, but i cant seem to find any biographical information on him is there anyway you can help me?

---------------------------------------------------------------------------

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From: W.Muss-at-salk.at
Date: Fri, 21 Oct 2005 08:17:41 -0500
Subject: [Microscopy] AskAMicroscopist: Re: inventor of polorizing microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Irene,
good morning !

Try the correct name of the guy, which in fact is } NICOL {,
Search for "Nicol William" or, perhaps better, Nicol prism....you will get
some results.....
eg. on WIKIPEDIA
http://en.wikipedia.org/wiki/Nicol_prism

==} }
"......It was invented in 1828 by William Nicol (1768-1851) of
Edinburgh...." etc..

Best regards

Wolfgang Muss


Personal Communication Data:
OR Dr. Wolfgang Muss Member of
EM-Lab
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively

Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o
W.Muss")
E-Mail work: W.Muss-at-SALK.at

Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at


----------
Von: familia_zepeda-at-yahoo.com[SMTP:familia_zepeda-at-yahoo.com]
Antwort an: familia_zepeda-at-yahoo.com
Gesendet: Freitag, 21. Oktober 2005 15:05
An: W.Muss-at-salk.at
Betreff: [Microscopy] AskAMicrosc: inventor of polarizing microscope




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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (familia_zepeda-at-yahoo.com) from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
Thursday, October 20, 2005 at 22:15:54
---------------------------------------------------------------------------

Email: familia_zepeda-at-yahoo.com
Name: Irene Zepeda

Organization: Canyon Springs High school

Education: 9-12th Grade High School

Location: North Las Vegas, Nevada 89030

Question: I am doing a research project on William Nichol who invented the
polarizing microscope, but i cant seem to find any biographical information
on him is there anyway you can help me?

---------------------------------------------------------------------------

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From: baskin-at-bio.umass.edu
Date: Fri, 21 Oct 2005 11:35:32 -0500
Subject: [Microscopy] Re: AskAMicroscopist:inventor of polorizing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Irene,

William Nicol invented the 'Nicol prism' in 1828. This polarizer prism is "a
rhombohedren of calcite, cut diagonally, ground and polished and cemented
together with Canada balsam". However not many living microscopists will
have ever used one, as Edwin Land subsequently invented 'Polaroid' plastic
sheet in 1932 and this is now always used in place of the Nicol prism as the
polarizer/analyser.

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk
----- Original Message -----
X-from: {familia_zepeda-at-yahoo.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Friday, October 21, 2005 2:05 PM





Hey...Watch it.

My first pol scope had nicol polarized and cap analyzer. I'm still using
that scope as the balsam hasn't separated. Oh, yes it has a mirror and I
use a focusable lamp. I used to do dispersion staining for asbestos and
microchemical test with that scope... Boy, thinking of it bring back
memories....

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
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keith.morris-at-ucl.
ac.uk To: frank.karl-at-degussa.com
cc:
10/21/2005 10:08 Subject: [Microscopy] Re: AskAMicroscopist:inventor of polorizing microscope
AM
Please respond to
keith.morris








----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Dear Irene,

William Nicol invented the 'Nicol prism' in 1828. This polarizer prism is
"a
rhombohedren of calcite, cut diagonally, ground and polished and cemented
together with Canada balsam". However not many living microscopists will
have ever used one, as Edwin Land subsequently invented 'Polaroid' plastic
sheet in 1932 and this is now always used in place of the Nicol prism as
the
polarizer/analyser.

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk
----- Original Message -----
X-from: {familia_zepeda-at-yahoo.com}
To: {keith.morris-at-ucl.ac.uk}
Sent: Friday, October 21, 2005 2:05 PM

Greetings,
I think that the Nicol type prism provides high quality
extinction, better than the Land type. I expect there have been
modern refinements to the design since Nicol but a pair of cemented
appropriately cut crystals provide excellent extinction. I believe
they are common on optical benches in physics applications -- it can
be awkward working the prisms into a microscope light path.

As ever,
Tobias

}
}
}
} Hey...Watch it.
}
} My first pol scope had nicol polarized and cap analyzer. I'm still using
} that scope as the balsam hasn't separated. Oh, yes it has a mirror and I
} use a focusable lamp. I used to do dispersion staining for asbestos and
} microchemical test with that scope... Boy, thinking of it bring back
} memories....
}
} Frank Karl
} Degussa Corporation
} Akron Technical Center
} 3500 Embassy Parkway
} Suite 100
} Akron, Ohio 44333
}
} 330-668-2235 Ext. 238
}
}
}
}
}
} keith.morris-at-ucl.
} ac.uk To:
} frank.karl-at-degussa.com
}
} cc:
} 10/21/2005 10:08 Subject:
} [Microscopy] Re: AskAMicroscopist:inventor of polorizing
} microscope
}
} AM
} Please respond
} to
} keith.morris
}
}
}
}
}
} Dear Irene,
}
} William Nicol invented the 'Nicol prism' in 1828. This polarizer prism is
} "a
} rhombohedren of calcite, cut diagonally, ground and polished and cemented
} together with Canada balsam". However not many living microscopists will
} have ever used one, as Edwin Land subsequently invented 'Polaroid' plastic
} sheet in 1932 and this is now always used in place of the Nicol prism as
} the
} polarizer/analyser.
}
} Regards
}
} Keith
}
} ----------------------------------------------------------
} Dr Keith J Morris
} Imaging Facilities Manager
} Cell Biology Division
} Institute of Ophthalmology
} University College London
} 11-43 Bath Street
} London EC1V 9EL
}
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

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4, 17 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
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From: neuberger1234-at-comcast.net
Date: Fri, 21 Oct 2005 12:28:25 -0500
Subject: [Microscopy] AskAMicroscopist:inventor of polorizing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Another problem with the plastic polarizing filters is that they fade
quickly or melt outright under intense light sources, e.g. a Xenon lamp. I
wonder if the cement used in the cut crystal prisms would also experience a
problem with high light intensity. BTW, I found that heat filters did not
sufficiently help; neutral density filters did help but those defeated the
purpose of the high intensity lamp we were trying to use.

Damian Neuberger, Ph.D.
Consultant
Microscopy/Digital Imaging/Image Analysis
2416 Covert Rd
Glenview IL 60025
Tel: (847) 998-8574
email: neuberger1234-at-comcast.net {mailto:neuberger1234-at-comcast.net}


----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Greetings,
I think that the Nicol type prism provides high quality
extinction, better than the Land type. I expect there have been
modern refinements to the design since Nicol but a pair of cemented
appropriately cut crystals provide excellent extinction. I believe
they are common on optical benches in physics applications -- it can
be awkward working the prisms into a microscope light path.

As ever,
Tobias




==============================Original Headers==============================
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From: woad-at-iinet.net.au
Date: Fri, 21 Oct 2005 17:25:16 -0500
Subject: [Microscopy] viaWWW: 3d phase contrast light microscopy

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (woad-at-iinet.net.au) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Friday, October 21, 2005 at 12:16:15
---------------------------------------------------------------------------

Email: woad-at-iinet.net.au
Name: Francois Burton-Bradley

Organization: Newcastle University

Title-Subject: [Filtered] 3d phase contrast light microscopy

Question: I remember running across a particular university group that had at one stage been working on generating 3d image from phase contrast LM images. However I can't find the link as I've forgotten it...does anybody know of any people/links/papers doing work in this area?

thanks

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From: mierzwa-at-jeol.com
Date: Fri, 21 Oct 2005 17:38:58 -0500
Subject: [Microscopy] MMMS workshop- Analytical Techniques in EM

Contents Retrieved from Microscopy Listserver Archives
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MMMS WORKSHOP - Analytical Techniques in EM

Presented by:
Midwest Microscopy and Microanalysis Society (MMMS)
Affiliate of the Microscopy Society of America and the Microbeam Analysis Society of America

November 18th, 2005
8:00AM - 4:45PM
Baxter Corporate Headquarters
Deerfield, IL


Registration Fees:
MMMS members: - $ 30.00
Non-members: - $40.00 (MMMS membership is included in fee)
MMMS students members: $15.00
Non-member students: $ 20.00 (MMMS membership is included in fee)

Vendors: We welcome vendors, tables for literature and exhibits are available. Contact us for details.

8:00AM - 8:30AM Setup and registration

8:45AM - 9:30AM Automatic Chemical Classification of Phases of Interest in the EM - John Friel, Princeton Gama Tech.
9:30AM - 10:15AM Factors Affecting Light Element Analysis - Neil Rowlands, Oxford Instruments NanoAnalysis

10:15 - 10:45 Break

10:45 AM - 11:30AM Parallel Beam Optics an Evaluation of Current Performance - Del Redfern, Edax, Inc.
11:30AM - 12:15PM Ultra high-energy-resolution Soft X-ray Emission Spectroscopy: seeing what EELS does not see. Yasuo Ito, Northern Illinois University

12:15PM - 1:15PM LUNCH - Included

1:15PM - 2:00PM Spectral and Spatial Analyses of Spectral Imaging Data Sets - Dr. Patrick Camus, Themo Electron Corporation
2:00PM - 2:45PM Electrical and refractory properties of nanowires measured by TEM - Oleg Lourie, Gatan, Inc.

2:45PM - 3:15PM Break

3:15PM - 4:00PM QuantomiX WETSEM Technology - Daphna Yaniv, QuantomiX
4:00PM - 4:45PM

RSVP is Requested by NOV 4th
Please send RSVP via email or phone to:


Robert Mierzwa
MMMS Past President
TEL (920) 803-8945
mierzwa-at-jeol.com



==============================Original Headers==============================
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From: wpchan-at-u.washington.edu
Date: Mon, 24 Oct 2005 01:43:26 -0500
Subject: [Microscopy] replies to Re: CPD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Couple weeks ago, I posted a question on whether it is necessary to have
enough (or any) ethanol to cover the sample in the CPD chamber at the
beginning of CPD. I received many replies and are grateful for the
advice. Here is a summary of the replies.

1. Filling the CPD chamber with ethanol is not necessary. In some cases,
there are enough ethanol in the CPD chamber and the specimen basket that
evaporation is minimal, if any. Some preparations are more tolerant to
this condition.

2. Filling the CPD chamber with ethanol is essential. The majority of the
replies recommends never letting the preparation exposed to air during
dehydration to avoid uneven evaporation, which will defeat the purpose of
doing CPD. Delicate specimens can probably benefit from a more cautious
approach.

3. One reply suggest using amyl acetate as the intermediate fluid after
ethanol dehydration, but I don't think I will use that because the need of
access to a fume hood. So far, ethanol and CO2 alone has given us good
results. At least for those preps that we were able to compare to live
specimens.

I will continue to suggest to users of our facility to be aware of the
condition mentioned in #2 above and hopefully the user will adopt the
appropriate practice. For my own preps, I will definitely go with #2.
Thanks again for sharing your expertise. Cheers.

--
Pang (Wai Pang Chan, wpchan-at-u.washington.edu)

==============================Original Headers==============================
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From: William.H.Roberts-at-USA.dupont.com
Date: Mon, 24 Oct 2005 11:15:36 -0500
Subject: [Microscopy] ReindeerGraphics.com

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear Listers,

I cannot access ReindeerGraphics.com this morning (publishers of Fovea Pro
image analysis plugin suite for Photoshop). Does anyone have information
as to the status of the web site? Perhaps someone has an 800 or other
area code number for their offices? Any help appreciated.

Thanks, Bill

This communication is for use by the intended recipient and contains
information that may be Privileged, confidential or copyrighted under
applicable law. If you are not the intended recipient, you are hereby
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of a contract offer. This e-mail does not constitute a consent to the
use of sender's contact information for direct marketing purposes or for
transfers of data to third parties.

Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean

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==============================Original Headers==============================
10, 22 -- From William.H.Roberts-at-USA.dupont.com Mon Oct 24 11:15:35 2005
10, 22 -- Received: from mms02bas.mms.us.syntegra.com (relay2.mms.us.syntegra.com [150.143.103.20])
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10, 22 -- From: William H Roberts {William.H.Roberts-at-USA.dupont.com}
10, 22 -- Subject: ReindeerGraphics.com
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From: William.H.Roberts-at-USA.dupont.com
Date: Mon, 24 Oct 2005 11:44:41 -0500
Subject: [Microscopy] Fw: ReindeerGraphics.com

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






----- Forwarded by William H Roberts/US/FILM/DPT on 10/24/05 12:41 PM -----

Brent Neal
{fbneal-at-mac.com}
To
10/24/05 12:38 PM William H Roberts/US/FILM/DPT-at-DPT
cc

Subject
Re: [Microscopy]
ReindeerGraphics.com










(10/24/05 11:18) William.H.Roberts-at-USA.dupont.com
{William.H.Roberts-at-USA.dupont.com} wrote:

} Dear Listers,
}
} I cannot access ReindeerGraphics.com this morning (publishers of Fovea Pro
} image analysis plugin suite for Photoshop). Does anyone have information
} as to the status of the web site? Perhaps someone has an 800 or other
} area code number for their offices? Any help appreciated.
}
} Thanks, Bill


You can reach the Reindeer Graphics offices at 919.342.0209. RGI's website
is hosted in Boca Raton, FL, which probably gives you some hint as to why
its not up right now.

B


P.S. Please forward this to the Microscopy list - I can't seem to send to
them right now. Thanks!
--
Brent Neal
Geek of all Trades
"Specialization is for insects" -- Robert A. Heinlein
http://brentn.freeshell.org

This communication is for use by the intended recipient and contains
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return e-mail and delete this e-mail from your system. Unless explicitly
and conspicuously designated as "E-Contract Intended", this e-mail does
not constitute a contract offer, a contract amendment, or an acceptance
of a contract offer. This e-mail does not constitute a consent to the
use of sender's contact information for direct marketing purposes or for
transfers of data to third parties.

Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean

http://www.DuPont.com/corp/email_disclaimer.html

==============================Original Headers==============================
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From: neuberger1234-at-comcast.net
Date: Mon, 24 Oct 2005 11:57:07 -0500
Subject: [Microscopy] Fw: ReindeerGraphics.com

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Forwarded per request.

Damian Neuberger

(10/24/05 11:18) William.H.Roberts-at-USA.dupont.com
{William.H.Roberts-at-USA.dupont.com} wrote:

} Dear Listers,
}
} I cannot access ReindeerGraphics.com this morning (publishers of Fovea Pro
} image analysis plugin suite for Photoshop). Does anyone have information
} as to the status of the web site? Perhaps someone has an 800 or other
} area code number for their offices? Any help appreciated.
}
} Thanks, Bill


You can reach the Reindeer Graphics offices at 919.342.0209. RGI's website
is hosted in Boca Raton, FL, which probably gives you some hint as to why
its not up right now.

B


P.S. Please forward this to the Microscopy list - I can't seem to send to
them right now. Thanks!
--
Brent Neal
Geek of all Trades
"Specialization is for insects" -- Robert A. Heinlein
http://brentn.freeshell.org



==============================Original Headers==============================
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From: opmills-at-mtu.edu
Date: Mon, 24 Oct 2005 15:01:56 -0500
Subject: [Microscopy] TEM preparation of zooplankton

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Can any of you provide me a reference or procedure for preparing
zooplankton for TEM + microanalysis?

Thanks!

Owen
Owen P. Mills
Michigan Technological University
Rm 512 M&M Bldg.
Houghton, MI 49931
PH 906-369-1875
FAX 906-487-2934
mailto:opmills-at-mtu.edu
http://www.mm.mtu.edu/~opmills




==============================Original Headers==============================
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7, 31 -- From: "Owen P. Mills" {opmills-at-mtu.edu}
7, 31 -- Subject: [MICROSCOPY]TEM preparation of zooplankton
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From: oshel1pe-at-cmich.edu
Date: Mon, 24 Oct 2005 15:16:28 -0500
Subject: [Microscopy] RE: TEM preparation of zooplankton

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Owen,

Look up the UNESCO guides on oceanographic methodology. They have one specifically on zooplankton fixation and preservation.
Also, I have this and another guide or two at home, still in boxes. I'll try to remember to bring them in so I can the references.
But ... what are you trying to fix and to analyze? If labile ions like Na, K, Ca and the like, you really need to use cryomethods for microanalysis. If the elements you're after are not labile, then standard Karnovsky's and so on may work.

Phil

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

----------

Hi,

Can any of you provide me a reference or procedure for preparing
zooplankton for TEM + microanalysis?

Thanks!

Owen
Owen P. Mills
Michigan Technological University
Rm 512 M&M Bldg.
Houghton, MI 49931
PH 906-369-1875
FAX 906-487-2934
mailto:opmills-at-mtu.edu
http://www.mm.mtu.edu/~opmills




==============================Original Headers==============================
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From: eeloe-at-ucsd.edu
Date: Mon, 24 Oct 2005 18:15:17 -0500
Subject: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eeloe-at-ucsd.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 24, 2005 at 17:34:46
---------------------------------------------------------------------------

Email: eeloe-at-ucsd.edu
Name: Emiley Eloe

Organization: Scripps Institution of Oceanography

Education: Graduate College

Location: La Jolla, CA USA

Question: I'm trying to prepare Vibrio parahaemolyticus samples for SEM on 2216-marine agar plates and am having difficulties visualizing the bacterial cells on the agar surface. I have used a 1% glut/0.1M cacodylic acid plus 3.5% salt fixative and fixed cells by gently pouring over the agar plate. It appears as though cells are somehow diffusing or migrating into the agar and avoiding being fixed directly on the surface. Any help would be appreciated.

---------------------------------------------------------------------------

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From: oshel1pe-at-cmich.edu
Date: Tue, 25 Oct 2005 07:27:37 -0500
Subject: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Carefully cut out 1 millimeter X ~10 mm X ~1 mm pieces of the agar where the bacteria are. Fix for a couple of hours at room temperature (or overnight in the 'frig) and dehydrate as usual. After the final 100% EtOH step, drop the pieces into liquid nitrogen. The EtOH freezes vitreously, and so won't cause any freezing artifacts. If the pieces don't spontaneously fall apart, break them with a razor blade. This will expose the bacteria in the agar. (This method also works for all sorts of things.) Return to 100% EtOH, then critical-point dry.

Phil

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576



-----Original Message-----
X-from: eeloe-at-ucsd.edu [mailto:eeloe-at-ucsd.edu]
Sent: Mon 24-Oct-05 19:21
To: Oshel, Philip Eugene

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eeloe-at-ucsd.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 24, 2005 at 17:34:46
---------------------------------------------------------------------------

Email: eeloe-at-ucsd.edu
Name: Emiley Eloe

Organization: Scripps Institution of Oceanography

Education: Graduate College

Location: La Jolla, CA USA

Question: I'm trying to prepare Vibrio parahaemolyticus samples for SEM on 2216-marine agar plates and am having difficulties visualizing the bacterial cells on the agar surface. I have used a 1% glut/0.1M cacodylic acid plus 3.5% salt fixative and fixed cells by gently pouring over the agar plate. It appears as though cells are somehow diffusing or migrating into the agar and avoiding being fixed directly on the surface. Any help would be appreciated.


==============================Original Headers==============================
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From: mjo10-at-psu.edu
Date: Tue, 25 Oct 2005 15:03:02 -0500
Subject: [Microscopy] Preparing Porous Sintered TEM Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A couple of months back I asked the listserver about preparing porous
samples for TEM, and the advice I received worked quite well. For
sake of the archival information, I wanted to comment on what
suggestions were made, which were applied and the results of the
work. This may be common knowledge for some, but it may be of some
assistance to others in the future. Any suggestions or comments are
also welcomed such that they will be included in the thread for
future searches.

Preparing TEM samples from sintered powders of metals or oxides
Problem:
Due to the large amount of porosity in certain sintered powder
samples, polishing or dimpling to a thickness of 10-15 um is
difficult because the sample has very little mechanical strength and
is susceptible to crack formation even at small forces.

Proposed Solutions:
Infiltrate with crazy glue or sulfur to fill in the porosity, thereby
making the sample more homogeneous. The rigidity of the infiltrated
media will assist in reducing the cracks formed within the sample
during polishing or dimpling.

Steps Taken:
Samples were polished down to 75-100 um and then placed in pure
acetone to remove any crystal bond from the pores. While crystal
bond did partially fill in the pores, it was not rigid enough to keep
the sample intact at final polishing steps. The samples may need to
be polished down farther before they are infiltrated with crazy glue
depending on the extent of the porosity. Typically though, if the
crazy glue does not fully penetrate the sample at 75-100 um, then the
sample is probably compact enough to polish to 10-15 um without infiltration.

Once removed from acetone, drops of crazy glue (store bought) were
placed on the sample until it appeared that it had stopped absorbing
the glue. The sample was then drawn across a piece of wax paper
until the bottom of the sample did not stick to the wax paper. This
was done to ensure that the sample remains flat so that when it is
remounted it is not uneven. The sample was then left to cure for the
recommended time and temperature for crazy glue (denoted on package).

A second, more ingenious, method was developed by a co-worker with
similar issues in that he cut a plastic capsule to the shape of a
small tube and stuck it down to double sided tape on a glass
slide. The sample was stuck inside the tube onto the tape (keeping a
flat base), and crazy glue was subsequently poured into the tube
covering the sample. The crazy glue was cured at the appropriate
time and temperature.

After curing, the samples were then polished down to ~10-15 um,
mounted on Cu slot grids and subsequently ion milled.

Addendum:
Morphologically (e.g., the thickness of a thin anodized layer or
shape of a sintered particulate), the presence of a polymer layer
during ion milling is definitely beneficial because it provides a
watermark for the oxide layer. That is to say, one can be assured
that the edge of an oxidized layer or particle is true, and has not
been milled away during preparation. Yet, for spectroscopy, the
samples we were analyzing required the absence of a carbon
signal. The presence of the crazy glue during ion milling tended to
give a thin film of redeposited carbon on the samples, thereby
confounding our data analysis. In this case, we infiltrated with
crazy glue in one of the aforementioned manners, polished the sample
to ~10-15 um, affixed them to Cu slot grids and finally put them
through a series of solution baths. As prescribed by an older email
on the listserver, the sample was bathed in pure acetone for 10
minutes, followed by a pure ethanol bath for 10 minutes and finally
bathed in pure (as pure as possible in one's lab) water for 10
minutes. Between each bath the sample was allowed to dry. The
acetone removes the remnants of the crazy glue. The ethanol helps to
remove any remaining acetone residue, and finally the water helps to
remove any ethanol residue.

Results:
After plasma cleaning the samples for 10 minutes (in the case of the
bathed samples), the reduction of C signal in our samples was greatly
reduced if not eliminated. In order to do STEM/EELS, a cold stage
was used to further reduce any carbon contamination from the
microscope. As previously mentioned, when the crazy glue was left
intact during ion milling, the morphology of the anodized thin films
could easily be observed.

Regards
Matt Olszta
Department of Materials Science and Engineering
Penn State University


==============================Original Headers==============================
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From: hbarwood-at-troy.edu
Date: Tue, 25 Oct 2005 15:14:37 -0500
Subject: [Microscopy] 23mm to 30mm eyepiece adapter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to mount a small webcam that I've adapted for NIR imaging in
one eyepiece of a Nikon SMZ-10 microscope. I'm using a 23mm eyepiece, and
the scope eyepiece is a 30mm size. Does anyone know of an inexpensive source
of an adapter ring?

Henry Barwood
Associate Professor of Science, Earth Science
Department of Math and Physics
MSCX 312G
Troy University
Troy, Alabama 36082
hbarwood-at-troy.edu


==============================Original Headers==============================
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3, 25 -- Subject: 23mm to 30mm eyepiece adapter
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From: phillipst-at-missouri.edu
Date: Tue, 25 Oct 2005 16:33:08 -0500
Subject: [Microscopy] Interesting Zeiss booklet on Abbe's lifework

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I recently received a copy of the Zeiss Innovation magazine which focuses
on Ernst Abbe's contributions to microscopy. In addition to some
interesting history, there are nice explanations of NA, immersion fluids
and magnification. The back of the book is typical ad material but the
front is worth a read. if you didn't get one, ask your zeiss rep or email
them. i have no financial interest in zeiss - i am just a user (sometimes
happy, sometimes not) of the product. Tom Phillips



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



==============================Original Headers==============================
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From: jmkrupp-at-cats.ucsc.edu
Date: Tue, 25 Oct 2005 18:56:16 -0500
Subject: [Microscopy] how to dehumidify?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi:

A lab user is having problems with ice crystals growing on cryo-grids
during transfers to the microscope.

Room humidity varies, but is typically 50%. Any tricks for reducing it and
ice crystal growth?

I thought about a room dehumidifier, but the ones I see say they only go
down to 30% and I don't know how effective it would be to try to do the
whole room.

Some kind of chamber with silica gel or ??.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



==============================Original Headers==============================
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From: walck-at-southbaytech.com
Date: Tue, 25 Oct 2005 21:00:28 -0500
Subject: [Microscopy] how to dehumidify?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jonathon,
There is an easy way to do this. Get a garbage bag and run a Tygon(R)
tube from a cylinder of nitrogen or from bleed off from liquid nitrogen.
Put your bag over your sample and put the tube into the bag and flush
the volume with nitrogen. Alternatively, you can use or newly
introduced Thing-A-Ma-Jug(TM) which can make a positive pressure of N2
or CO2 from the head space of a Dewar of liquid nitrogen or dry ice.
Transport your sample under the bag with the nitrogen to the TEM and
work under the bag. (Note, I said under the bag, not in the bag.) I
think that you can keep the humidity under the bag close to 0%.

If you use a high flow of N2, please make sure that you have adequate
ventilation.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Tuesday, October 25, 2005 5:02 PM
To: Walck-at-SouthBayTech.com

Hi:

A lab user is having problems with ice crystals growing on cryo-grids
during transfers to the microscope.

Room humidity varies, but is typically 50%. Any tricks for reducing it
and ice crystal growth?

I thought about a room dehumidifier, but the ones I see say they only go
down to 30% and I don't know how effective it would be to try to do the
whole room.

Some kind of chamber with silica gel or ??.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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21, 24 -- From walck-at-southbaytech.com Tue Oct 25 21:00:28 2005
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From: gcc-at-couger.com
Date: Tue, 25 Oct 2005 22:35:21 -0500
Subject: [Microscopy] Re: how to dehumidify?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



jmkrupp-at-cats.ucsc.edu wrote:
} Hi:
}
} A lab user is having problems with ice crystals growing on cryo-grids
} during transfers to the microscope.
}
} Room humidity varies, but is typically 50%. Any tricks for reducing it and
} ice crystal growth?
}
} I thought about a room dehumidifier, but the ones I see say they only go
} down to 30% and I don't know how effective it would be to try to do the
} whole room.
}
} Some kind of chamber with silica gel or ??.
}
} Thanks
}
} Jon
}
Put a dehumidifier or large boxes of dehumidifying agent than you can
regenerate with heat in a plastic tent over his work station or even a
smaller tent around the scope. The humidly is much easier and cheaper to
control in a small space and two make an airlock design so other
experiments in the room don't mess with yours.

In cold rooms the space is usually filled with work sooner than later.

Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org



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From: rcsaic-at-sbcglobal.net
Date: Wed, 26 Oct 2005 09:17:17 -0500
Subject: [Microscopy] General TEM: Software for generating SAD patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What software is currently available, commercial or freeware, that will
generate single-crystal selected-area diffraction patterns for a given
crystal for a given zone axis, given its cell parameters and atomic
positions etc? The Desktop Microscopist product used to do this, but it
seems to have disappeared from the landscape in the intervening last few
years that I've been away from microscopy (can't find it on Google). I
remember that most HRTEM multislice image simulation programs will generate
SA or CBED patterns, but I'm looking for something a little simpler.

Thanks,

Roy Christoffersen
SAIC
Code KA-Astromaterials Directorate
NASA Johnson Space Center



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From: YANGA-at-AGR.GC.CA
Date: Wed, 26 Oct 2005 09:21:37 -0500
Subject: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





The advice from Dr. Oshel does not solve the problem that the bacteria would not stay together during fixation in aqueous glutaraldehyde. When I wanted to see the surface of a colony, I fixed the small pieces of the agar gel with the colonies on them in osmium tetroxide vapour in a humid chamber and then carefully dehydrated them in a graded ethanol series.

If the original writer only wants to get images of the bacteria irrespective whether they are from the top or the bottom of the colony, it would be advisable to gently release (from a pasteur pipette) a thin layer of } 40°C 3-4% agar sol. (I used to incorporate glutaraldehyde into the agar sol before use). This coating would immobilize the bacteria in the colony and the sample would be dehydrated and critical-point dried as usual. The dried agar coating would be opened easily before mounting the bacteria on a stub.

You may provide this kind of advice and you do not need to mention my name.
Regards,

Milos

Note: Milos is a retired scientist.

Ann Fook

-----Original Message-----
X-from: Yang, Ann-Fook
Sent: Wednesday, October 26, 2005 9:54 AM
To: Kalab, Miloslav

Carefully cut out 1 millimeter X ~10 mm X ~1 mm pieces of the agar where the bacteria are. Fix for a couple of hours at room temperature (or overnight in the 'frig) and dehydrate as usual. After the final 100% EtOH step, drop the pieces into liquid nitrogen. The EtOH freezes vitreously, and so won't cause any freezing artifacts. If the pieces don't spontaneously fall apart, break them with a razor blade. This will expose the bacteria in the agar. (This method also works for all sorts of things.) Return to 100% EtOH, then critical-point dry.

Phil

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576



-----Original Message-----
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Sent: Mon 24-Oct-05 19:21
To: Oshel, Philip Eugene

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eeloe-at-ucsd.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 24, 2005 at 17:34:46
---------------------------------------------------------------------------

Email: eeloe-at-ucsd.edu
Name: Emiley Eloe

Organization: Scripps Institution of Oceanography

Education: Graduate College

Location: La Jolla, CA USA

Question: I'm trying to prepare Vibrio parahaemolyticus samples for SEM on 2216-marine agar plates and am having difficulties visualizing the bacterial cells on the agar surface. I have used a 1% glut/0.1M cacodylic acid plus 3.5% salt fixative and fixed cells by gently pouring over the agar plate. It appears as though cells are somehow diffusing or migrating into the agar and avoiding being fixed directly on the surface. Any help would be appreciated.


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From: oshel1pe-at-cmich.edu
Date: Wed, 26 Oct 2005 11:04:04 -0500
Subject: [Microscopy] AskAMicroscopist: Vibrio parahaemolyticus samples for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It won't? Odd. I've used the fix-EtOH cryofracture methods many times to study bacteria and yeast within (and on) agar without the critters "not stay[ing] together". The glutaraldehyde fixation step fixes the bacteria in place quite nicely.
Phil

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


The advice from Dr. Oshel does not solve the problem that the bacteria would not stay together during fixation in aqueous glutaraldehyde. When I wanted to see the surface of a colony, I fixed the small pieces of the agar gel with the colonies on them in osmium tetroxide vapour in a humid chamber and then carefully dehydrated them in a graded ethanol series.

If the original writer only wants to get images of the bacteria irrespective whether they are from the top or the bottom of the colony, it would be advisable to gently release (from a pasteur pipette) a thin layer of } 40°C 3-4% agar sol. (I used to incorporate glutaraldehyde into the agar sol before use). This coating would immobilize the bacteria in the colony and the sample would be dehydrated and critical-point dried as usual. The dried agar coating would be opened easily before mounting the bacteria on a stub.

You may provide this kind of advice and you do not need to mention my name.
Regards,

Milos

Note: Milos is a retired scientist.

Ann Fook

-----Original Message-----
X-from: Yang, Ann-Fook
Sent: Wednesday, October 26, 2005 9:54 AM
To: Kalab, Miloslav

Carefully cut out 1 millimeter X ~10 mm X ~1 mm pieces of the agar where the bacteria are. Fix for a couple of hours at room temperature (or overnight in the 'frig) and dehydrate as usual. After the final 100% EtOH step, drop the pieces into liquid nitrogen. The EtOH freezes vitreously, and so won't cause any freezing artifacts. If the pieces don't spontaneously fall apart, break them with a razor blade. This will expose the bacteria in the agar. (This method also works for all sorts of things.) Return to 100% EtOH, then critical-point dry.

Phil

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576



-----Original Message-----
X-from: eeloe-at-ucsd.edu [mailto:eeloe-at-ucsd.edu]
Sent: Mon 24-Oct-05 19:21
To: Oshel, Philip Eugene

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (eeloe-at-ucsd.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, October 24, 2005 at 17:34:46
---------------------------------------------------------------------------

Email: eeloe-at-ucsd.edu
Name: Emiley Eloe

Organization: Scripps Institution of Oceanography

Education: Graduate College

Location: La Jolla, CA USA

Question: I'm trying to prepare Vibrio parahaemolyticus samples for SEM on 2216-marine agar plates and am having difficulties visualizing the bacterial cells on the agar surface. I have used a 1% glut/0.1M cacodylic acid plus 3.5% salt fixative and fixed cells by gently pouring over the agar plate. It appears as though cells are somehow diffusing or migrating into the agar and avoiding being fixed directly on the surface. Any help would be appreciated.


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From: terry-at-AsylumResearch.com
Date: Wed, 26 Oct 2005 13:08:17 -0500
Subject: [Microscopy] AFM Technical Sales Positions Available

Contents Retrieved from Microscopy Listserver Archives
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Asylum Research, a premier manufacturer of atomic force microscopes,
has two positions available for East Coast Technical Sales.
Information can be found http://www.asylumresearch.com/About/
About.shtml#CR

Terry Mehr
Asylum Research
www.AsylumResearch.com

==============================Original Headers==============================
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From: alan.wood-at-JUSTIS.COM
Date: Thu, 27 Oct 2005 08:04:38 -0500
Subject: [Microscopy] RE: viaWWW: M7A Yellow blue edging on image

Contents Retrieved from Microscopy Listserver Archives
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Curt Lamberth wrote:

} I have a Wild M7A stereomicroscope and the image(s) are edged
} with yellow on one side and, if you close one eye, blue on
} the other. This causes a yellow cast to object edges and is
} very annoying.
}
} I am using either x20 or x10 eyepieces, with LED or f-optic
} ring illumination. The problem is most obvious under higher
} magnifications.
}
} Does anyone know why this happens, and how it can be solved?

I expect I will get hate mail from Wild fans, but I think you are seeing
chromatic aberration. There is nothing you can do about it, except buy a
better microscope.

I am not familiar with the M7A, but in my last job I used several Wild M5
stereos. One of them showed yellow/blue aberration, and the others showed
red/green aberration. They were all regularly serviced by Leitz.

The Zeiss stereos used by the other entomologists, and the Olympus SZ-III
stereos used for training courses, did not show any obvious chromatic
aberration.

--
Alan Wood
http://www.alanwood.net (Unicode, special characters, pesticide names)

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From: kjl226-at-vt.edu
Date: Thu, 27 Oct 2005 08:31:24 -0500
Subject: [Microscopy] viaWWW: Protcol for embedding whole mosquitos

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (kjl226-at-vt.edu) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Wednesday, October 26, 2005 at 09:49:15
---------------------------------------------------------------------------

Email: kjl226-at-vt.edu
Name: Kathy lowe

Organization: Virginia Tech, College of Vet. Medicine

Title-Subject: [Filtered] MListserver: Protcol for embedding whole mosquitos

Question: Does anyone have a procedure for processing and embedding whole
mosquitos for TEM?
Kathy Lowe

---------------------------------------------------------------------------

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From: RW-at-web.de
Date: Thu, 27 Oct 2005 08:33:15 -0500
Subject: [Microscopy] AskAMicroscopist: embeding alive yeastcells

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (RW-at-web.de) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, October 27, 2005 at 05:24:16
---------------------------------------------------------------------------

Email: RW-at-web.de
Name: Ramona Wesselman

Organization: Center for Nanotchnology

Education: Graduate College

Location: Rheine, germany

Question: Hallo,
I am working with Yeast, I want to know if you treid to use Citifluor for embeding alive yeastcells, or if you can give me an advice for another embeding medium which is possible for yeast cells and has an index of refraction of 1,46 . Thanks!

---------------------------------------------------------------------------

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From: MAOKeefe-at-lbl.gov
Date: Thu, 27 Oct 2005 09:04:21 -0500
Subject: [Microscopy] Re: General TEM: Software for generating SAD

Contents Retrieved from Microscopy Listserver Archives
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Roy,

Probably the simplest way is to use an HREM image and diffraction
simulation package.

The UIUC code for electron microscopy is at:
http://emaps.mrl.uiuc.edu/

You could try the diffraction and image simulation software from
HREMresearch: http://www.hremresearch.com/Eng/simulation.html

If you just need a quick calculation now and then, consider EMS at
http://cimesg1.epfl.ch/CIOL/ems.html

A much "heavier" package is the Cerius:
http://cimewww.epfl.ch/EMYP/comp_sim.html
but you'll need some "cerious" money!

Mike O'Keefe
Director, MSA


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From: TindallR-at-missouri.edu
Date: Thu, 27 Oct 2005 12:43:35 -0500
Subject: [Microscopy] Immuno EM---GFP

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I'm curious to see if anyone has any words of wisdom about
immunostaining of GFP in general. I'd be interested in experiences
relating to DAB-HRP labeling and/or immunogold, both pre- and
post-embedding. Cryosections, too! (There, that ought to keep you
busy.)

More specifically, are there any danger spots in the fixation or
embedding processes that could negatively affect the labeling process,
or even destroy the protein itself?

I know it's one of those overly broad questions, but I am mainly
interested in how, if at all, labeling GFP might be different that
labeling other proteins. I've not had much luck going through our
library on finding GFP-specific material, and I'm still Googling away.

By the way, you're all invited to dinner at my place tonight by way of
thanks. My Honduran in-laws are in town and the food is good and
plentiful and the music is Latin. Yes, people CAN cook and dance at the
same time.

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: zaluzec-at-microscopy.com
Date: Thu, 27 Oct 2005 13:24:04 -0500
Subject: [Microscopy] Re: Software for generating SAD patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Roy

There is also the commerical suite of programs

Crystal Maker, Single Crystal, Crystal Diffract.

http://www.crystalmaker.com

Your Friendly Neighborhood SysOp
Nestor

Disclaimer: I have no financial interests in any of these programs
I do however use them.



==============================Original Headers==============================
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From: phillipst-at-missouri.edu
Date: Thu, 27 Oct 2005 14:18:53 -0500
Subject: [Microscopy] Re: Immuno EM---GFP

Contents Retrieved from Microscopy Listserver Archives
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Randy - Check out Grabenbauer et al (1005) Nature Methods 2(11):857-
Correlative microscopy and electron tomography of GFP through
photooxidation - they use GFP bleaching to drive DAB oxidation into an
electron dense precipitate.

I am old enough to have done a fair amount of DAB immunolabeling at the EM
level. It is much less satisfying than colloidal gold. So maybe a gold
method would be better in some circumstances.

GFP survives PF but not ethanol.

And while I believe that people can dance and cook at the same time, I have
yet to see you dance whenever I have been at your home. Tom



At 12:46 PM 10/27/05, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: TrogadisJ-at-smh.toronto.on.ca
Date: Thu, 27 Oct 2005 16:09:45 -0500
Subject: [Microscopy] heating live cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fellow Microscopists

We are looking for a device for maintaining a constant temperature
inside a plexiglass incubator which is installed around the stage of an
inverted microscope. Volume around 2 cu. ft.

We now have an external heating unit with temperature control that
blows warm air into the incubator, however the air then flows out
through small openings and it is difficult to regulate the CO2 levels
because the air is constantly changing. Besides, the warm air results in
fluid evaporation.

Ideally, I'm looking for a radiant source of heat to place inside the
incubator - no light bulbs, for obvious reasons. We do have a thermistor
to regulate the temperature into which the device could be plugged.

Any suggestions?
Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8
Canada
ph: 416-864-6060 x6337
pager: 416-685-9219
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca


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From: Rosemary.White-at-csiro.au
Date: Thu, 27 Oct 2005 16:56:12 -0500
Subject: [Microscopy] Re: Immuno EM---GFP

Contents Retrieved from Microscopy Listserver Archives
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Dear Randy,
Haven't done immunolabelling on tissues or sections, but the molecular
biologists here use anti-GFP and standard procedures to detect their
labelled proteins on westerns. These are not native gels, just standard
SDS-PAGE gels blotted onto nitrocellulose membrane, so the protein is
denatured.
cheers,
Rosemary

Rosemary White rosemary.white-at-csiro.au
CSIRO Plant Industry ph. 02-6246 5475
GPO Box 1600 mob. 0402 835 973
Canberra, ACT 2601 fax. 02-6246 5334
Australia


} From: TindallR-at-missouri.edu
} Reply-To: TindallR-at-missouri.edu
} Date: Thu, 27 Oct 2005 12:50:03 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] Immuno EM---GFP
}
}
}
}
} ----------------------------------------------------------------------------
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} Dear Listers,
}
} I'm curious to see if anyone has any words of wisdom about
} immunostaining of GFP in general. I'd be interested in experiences
} relating to DAB-HRP labeling and/or immunogold, both pre- and
} post-embedding. Cryosections, too! (There, that ought to keep you
} busy.)
}
} More specifically, are there any danger spots in the fixation or
} embedding processes that could negatively affect the labeling process,
} or even destroy the protein itself?
}
} I know it's one of those overly broad questions, but I am mainly
} interested in how, if at all, labeling GFP might be different that
} labeling other proteins. I've not had much luck going through our
} library on finding GFP-specific material, and I'm still Googling away.
}
} By the way, you're all invited to dinner at my place tonight by way of
} thanks. My Honduran in-laws are in town and the food is good and
} plentiful and the music is Latin. Yes, people CAN cook and dance at the
} same time.
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
}
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From: jmkrupp-at-cats.ucsc.edu
Date: Thu, 27 Oct 2005 19:06:29 -0500
Subject: [Microscopy] LD vs 'regular' LM objectives

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Laura,

I haven't seen any replies to your question on the Microscopy
Listserver, so I thought I'd ask a biologist friend of mine about what
procedure scientists use to look at cancer cells under the microscope.
Of course, someone (probably much more knowledgable than I) may have
replied to you off-server.

Anyway, to detect cancer, pathologists use light (optical) microscopes
to look at slices of tissue on glass slides stained to show internal
cell organelles, and sometimes relationships of cells to other cells.

Sometimes, they may examine suspensions of blood or bone marrow cells
spread on glass slides and stained to detect unusual features. These
doctors are experts in knowing what normal tissues and cells look like
and can detect unusual features, such as enlarged and or strangely
convoluted nuclei, unusually long and numerous microvilli, or cells
invading tissue where they should not be located.

Additionally, they may order special stains to detect kinds of antigens
that are not found on normal tissue or that show up in unusual
locations, for example, whether breast or prostate tissue is found in
some other organ. Some pathologists and researchers use the electron
microscope to detect ultrafine structure (structure smaller than you see
with an optical microscope) inside cells -- such as premelanosomes,
desmosomes, or secretory granules -- to identify different types of
tumor cells. It is very important for the doctors to know the kind of
cancer is present because often different types respond to different
therapies.

Other experts doing research on tissues and cell cultures may use
special forms of optical microscopy such as fluorescence or confocal
microscopy to detect the presence of particular structures or locate
special antigens in cells. Researchers can contribute to the knowledge
of how cancer cells live and therefore what kinds of agents might be
used to kill them.

I hope that helps.

Mike O'Keefe
MSA Director

----- Original Message -----
X-from: loz_jay01-at-hotmail.com

Hi

Anyone like to offer an opinion of the differences in image quality between
LD and standard objective lenses for light microscopy including
fluorescence?

We have a 'hybrid' scope, some LD, some standard objectives and are trying
to decide which kind to add to go to make them all the same.

Pretty general question, I know, but give it a shot if you have some ideas.

Thanks

Jon


Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: payton-at-auburn.edu
Date: Thu, 27 Oct 2005 19:31:37 -0500
Subject: [Microscopy] viaWWW: Coating Ciruit Board cross-sections for SEM analysis

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---------------------------------------------------------------------------

Email: payton-at-auburn.edu
Name: Lewis Payton

Organization: Auburn University

Title-Subject: [Filtered] MListserver: Coating Ciruit Board cross-sections for SEM analysis

Question: Hello,

Can anyone recommend a system for carbon coating or gold coating an SEM specimen.

We are cross-sectioning circuit boards to analyze the solder balls. Unfortunately, the circuit board edging is non-conductive and scattering the beam.

We've used a gold coater and a carbon coater with good success, but buying one is prohibitively expensive it seems (as is renting the one we are using per sample).

I'd greatly appreciate any advice anyone might have in general.

Thank you,
Lewis



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From: avallecorsa-at-ups.edu
Date: Thu, 27 Oct 2005 19:32:00 -0500
Subject: [Microscopy] viaWWW: Emscope SP2000

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Email: avallecorsa-at-ups.edu
Name: Al vallecorsa

Organization: University of Puget Sound

Title-Subject: [Filtered] MListserver:

Question: We have an old Emscope SP2000, that we would like to get working. Can anyone supply a manual or information about it. One of our professors bought it used on E-bay.
Thank You, Al

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From: dhorne-at-interchange.ubc.ca
Date: Thu, 27 Oct 2005 19:32:26 -0500
Subject: [Microscopy] viaWWW: Platelet isolation for SEM

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Email: dhorne-at-interchange.ubc.ca
Name: Derrick Horne

Organization: University of British Columbia

Title-Subject: [Filtered] MListserver: Platelet isolation for SEM

Question: Anyone have experience isolating platelets (non-adherent) without activating them for SEM imaging?

I need a method for a 2nd year student and I'm not sure in what direction to take him. He has already tried fixing the plasma, with the expected gelatinous goopy result. How about diluting the PRP (platelet-rich plasma) with PB/saline and filtering it prior to fixation?

I've come across a size-exclusion chromatography method, but we are not setup to do that in our lab.

Any ideas would be appreciated.

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From: mpease-at-jhmi.edu
Date: Thu, 27 Oct 2005 19:32:52 -0500
Subject: [Microscopy] viaWWW: osmolarity of PBS

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Email: mpease-at-jhmi.edu
Name: Mary Ellen Pease

Organization: Johns Hopkins University School of Medicine

Title-Subject: [Filtered] MListserver:

Question: I am interested in learning the osmolarity of PBS as compared to 0.1M Sorenson's Phosphate buffer. I can find osmolarity info on Sorenson's but not on PBS. A colleague of mine perserved tissues intended for epoxy processing in fixative made in PBS rather than Sorenson's. Any idea if this will compromise the morphology in 1um, tol blue stained sections?

thanks!

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From: W.Muss-at-salk.at
Date: Fri, 28 Oct 2005 02:07:09 -0500
Subject: [Microscopy] Re: osmolarity of PBS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning,
dear Mary Ellen,

osmolarity of hydrous solutions, especially of "buffers" - as I know the
item - always depends on concentration of } effective { ions in solution.
So, in the case of 0.1 M Soerensen buffer you will have in solution a
certain amount of ions (and, because it is a PO4 buffer, cf. dissociation
of substances/ions, you will have some material which is not dissolved/in
solution).... - as you said - you will have some measure(s) on the
osmolarity/osmolality of (assuming 0.15M) Soerensen phosphate buffer--} how
much is your measure for this solution?.

If you have "PBS", you have to know the concentration (in M) or have to
calculate ionic pressure by the amount of buffer salt(s) in the solution.

Also not to forget that higher concentrated PO4-buffer solutions (being it
either NaOH-NaH2PO4 or Na2HPO4-NaH2PO4-mixtures) you hardly will be able
to measure correctly, e.g. by an osmometer....this is due to the uncomplete
dissociation of the PO4 ions if the ionic strength is high (e.g.0.2M: here
you will not get an exact AND reproducible mosmol-value). If you dilute
your buffer, say to 0.1M, or 0.05M you will see that measurement by an
osmometer will result in a (more) stable and therefore correct value.

As a classical "measure" you will find } Millonig's { 0.13 M NaOH-Na2HPO4
-buffer at around 290-295 mosmol, and if you are adding some Sucrose
(Saccharose) or better Glucose, you will get a 0.13 M NaPO4 buffer (pH
around 7.2-7.4) amounting 300 mosmol (isotonicity of human blood).

If you need further information on that, please specify the concentration
of your PBS (or ingredients), perhaps one can calculate the osmolarity

best regards

Wolfgang Muss

Personal Communication Data:
OR Dr. Wolfgang Muss Member of
EM-Lab
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively

Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o
W.Muss")
E-Mail work: W.Muss-at-SALK.at

Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at

----------
Von: mpease-at-jhmi.edu[SMTP:mpease-at-jhmi.edu]
Antwort an: mpease-at-jhmi.edu
Gesendet: Freitag, 28. Oktober 2005 02:39
An: W.Muss-at-salk.at
Betreff: [Microscopy] osmolarity of PBS


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Email: mpease-at-jhmi.edu
Name: Mary Ellen Pease

Organization: Johns Hopkins University School of Medicine

Title-Subject: [Filtered] MListserver:

Question: I am interested in learning the osmolarity of PBS as compared to
0.1M Sorenson's Phosphate buffer. I can find osmolarity info on Sorenson's
but not on PBS. A colleague of mine perserved tissues intended for epoxy
processing in fixative made in PBS rather than Sorenson's. Any idea if this
will compromise the morphology in 1um, tol blue stained sections?

thanks!

---------------------------------------------------------------------------

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==============================Original Headers==============================
25, 29 -- From W.Muss-at-salk.at Fri Oct 28 02:07:09 2005
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25, 29 -- "'microscopy-at-microscopy.com'"
25, 29 -- {microscopy-at-microscopy.com}
25, 29 -- Subject: [Microscopy] Re: osmolarity of PBS
25, 29 -- Date: Fri, 28 Oct 2005 09:06:57 +0200
25, 29 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at}
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From: mike.wombwell-at-quorumtech.com
Date: Fri, 28 Oct 2005 06:05:09 -0500
Subject: [Microscopy] viaWWW: Emscope SP2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Al,

We - Quorum Technologies - can mail you a copy of the original SP2000
operating manual, including wiring diagrams. Please be aware that the
SP2000 was discontinued around 15 years ago and so availability or
spares is likely to be limited. You may like to contact our local (US)
distributor Energy Beam Sciences (http://www.ebsciences.com/) for
further information and assistance.

Our relationship to "Emscope" products is now somewhat tenuous, but
back in 1988 the UK based Emscope company was acquired by Bio-rad, the
then owners of the Polaron range - now manufactured by Quorum
Technologies (for a potted history see:
http://www.quorumtech.com/history.htm)

Incidentally, many operating manuals for old products are available as
downloads from our website - including the Emscope SC500 / SC500A
sputter coater (see:
http://www.quorumtech.com/Tech_Support/old-manuals.htm)

Best regards,

Mike Wombwell
Sales & Marketing Director
Quorum Technologies
Newhaven, East Sussex, UK
Tel: +44(0)1273 510535
Tel: +44(0)1273 511063 (direct)
Fax: +44(0)1273 510536
mike.wombwell-at-quorumtech.com
http://www.quorumtech.com
E & O E








-----Original Message-----
X-from: avallecorsa-at-ups.edu [mailto:avallecorsa-at-ups.edu]
Sent: 28 October 2005 01:46
To: Mike Wombwell

Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (avallecorsa-at-ups.edu) from
http://www.microscopy.com/MLFormMail.html on Thursday, October 27, 2005
at 11:21:06
------------------------------------------------------------------------
---

Email: avallecorsa-at-ups.edu
Name: Al vallecorsa

Organization: University of Puget Sound

Title-Subject: [Filtered] MListserver:

Question: We have an old Emscope SP2000, that we would like to get
working. Can anyone supply a manual or information about it. One of our
professors bought it used on E-bay.
Thank You, Al

------------------------------------------------------------------------
---

==============================Original
Headers==============================
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==============================Original Headers==============================
29, 23 -- From mike.wombwell-at-quorumtech.com Fri Oct 28 06:05:08 2005
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From: paults3gj-at-yahoo.com
Date: Fri, 28 Oct 2005 07:50:23 -0500
Subject: [Microscopy] viaWWW: ISI TV mini-SEM documentation needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (paults3gj-at-yahoo.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, October 28, 2005 at 00:58:05
---------------------------------------------------------------------------

Email: paults3gj-at-yahoo.com
Name: Paul S

Organization: CWRU

Title-Subject: [Filtered] MListserver: ISI TV mini-SEM documentation needed

Question: I have an ISI TV mini-SEM model M-RS-2-2 made in 1975. It appears to have all the required parts. I'm trying to get this unit operating and need service documentation. I'd be happy to just get a copy of the complete electrical schematic.

If anyone knows where to get documentation or spare parts for this SEM or if anyone could provide me with an eletrical schematic, please let me know.

If anyone has used this SEM and could give me their general impression of its performance (mag, resolution, etc), I'd also appreciate it.

Thanks in advance.

Paul

---------------------------------------------------------------------------

==============================Original Headers==============================
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10, 12 -- Subject: viaWWW: ISI TV mini-SEM documentation needed
10, 12 -- Content-Type: text/plain; charset="us-ascii"
==============================End of - Headers==============================




From: ludovic.pinier-at-thalesgroup.com
Date: Fri, 28 Oct 2005 07:51:00 -0500
Subject: [Microscopy] viaWWW: TOPCON ABT-150F parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (ludovic.pinier-at-thalesgroup.com) from http://www.microscopy.com/MLFormMail.html on Friday, October 28, 2005 at 03:21:50
---------------------------------------------------------------------------

Email: ludovic.pinier-at-thalesgroup.com
Name: Ludovic Pinier

Title-Subject: [Filtered] looking for TOPCON ABT-150F parts

Question: Hello microscopists,

I work on a TOPCON ABT-150F microscope. It seems to be a quite rare SEM, that is no more supported by the manufacturer. I need gaskets because of filament replacement and I don't know if they still are commercially available.

Does anyone knowing that microscope can tell me where to find replacement parts ?

Thanks,
Ludovic Pinier



---------------------------------------------------------------------------

==============================Original Headers==============================
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From: phillipst-at-missouri.edu
Date: Fri, 28 Oct 2005 08:22:09 -0500
Subject: [Microscopy] Re: LD vs 'regular' LM objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The quality of the image and its ability to capture light (i.e., brightness
of fluorescence images) is determined by the numerical aperture (NA) and
the magnification of the objective. Long working distance objectives
generally have a lower NA so they produce lower resolution and less bright
fluorescent images than standard working distance objectives with similar
magnifications but higher NA's. For a given magnification, the higher the
NA, the brighter and sharper the image. For a given NA, the lower the
magnification, the brighter the image but I think you need to know the
actual NA and mag to predict the effect on resolution.

At 07:07 PM 10/27/05, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



==============================Original Headers==============================
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9, 21 -- From: Tom Phillips {phillipst-at-missouri.edu}
9, 21 -- Subject: Re: [Microscopy] LD vs 'regular' LM objectives
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From: YANGA-at-AGR.GC.CA
Date: Fri, 28 Oct 2005 08:34:02 -0500
Subject: [Microscopy] heating live cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Judy,

I have heard from my electronic colleague that there are heating-tape, heating-cable, heating-pad, and heating-block you can use. Heating-tape of different lengths may be purchased at Canadian Tire. The length you need depends upon the dimensions, temperature requirement, and heat loss (insulated?). I believe there is an electronic shop, in you hospital, where you can seek help. Otherwise, write to me off list, I will ask my colleague to do some calculation and advise what the best choice is.

Ann Fook Yang
EM Unit/ Unite EM
AAFC/AAC
960 Carling Ave,
Ottawa,Ontario
Canada K1A 0C6
yanga-at-agr.gc.ca
Telephone/Téléphone: 613-759-1638
Facsimile/Télécopieur: 613-759-1701

Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada

-----Original Message-----
X-from: TrogadisJ-at-smh.toronto.on.ca [mailto:TrogadisJ-at-smh.toronto.on.ca]
Sent: Thursday, October 27, 2005 5:12 PM
To: Yang, Ann-Fook

Fellow Microscopists

We are looking for a device for maintaining a constant temperature
inside a plexiglass incubator which is installed around the stage of an
inverted microscope. Volume around 2 cu. ft.

We now have an external heating unit with temperature control that
blows warm air into the incubator, however the air then flows out
through small openings and it is difficult to regulate the CO2 levels
because the air is constantly changing. Besides, the warm air results in
fluid evaporation.

Ideally, I'm looking for a radiant source of heat to place inside the
incubator - no light bulbs, for obvious reasons. We do have a thermistor
to regulate the temperature into which the device could be plugged.

Any suggestions?
Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8
Canada
ph: 416-864-6060 x6337
pager: 416-685-9219
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca


==============================Original Headers==============================
7, 19 -- From trogadisj-at-smh.toronto.on.ca Thu Oct 27 16:09:45 2005
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7, 19 -- From: "Judy Trogadis" {TrogadisJ-at-smh.toronto.on.ca}
7, 19 -- To: {Microscopy-at-msa.microscopy.com}
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==============================Original Headers==============================
15, 29 -- From YANGA-at-AGR.GC.CA Fri Oct 28 08:34:01 2005
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From: jquinn-at-www.matscieng.sunysb.edu
Date: Fri, 28 Oct 2005 08:43:20 -0500
Subject: [Microscopy] re: Heating live cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Omega" is the first source in temperature.

www.omega.com

regards,

Jim





} From mail-at-ns.microscopy.com Fri Oct 28 09:37:17 2005
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} Date: Fri, 28 Oct 2005 08:34:35 -0500
} Message-Id: {200510281334.j9SDYZ2c022619-at-ns.microscopy.com}
} To: jquinn-at-www.matscieng.sunysb.edu
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} Reply-to: YANGA-at-AGR.GC.CA
} X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} Subject: [Microscopy] RE: heating live cells
} Errors-To: MicroscopyListSpamFilter-at-microscopy.com
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} Status: R
}
}
}
}
} ----------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi Judy,
}
} I have heard from my electronic colleague that there are heating-tape, heating-cable, heating-pad, and heating-block you can use. Heating-tape of different lengths may be purchased at Canadian Tire. The length you need depends upon the dimensions, temperature requirement, and heat loss (insulated?). I believe there is an electronic shop, in you hospital, where you can seek help. Otherwise, write to me off list, I will ask my colleague to do some calculation and advise what the best choice is.
}
} Ann Fook Yang
} EM Unit/ Unite EM
} AAFC/AAC
} 960 Carling Ave,
} Ottawa,Ontario
} Canada K1A 0C6
} yanga-at-agr.gc.ca
} Telephone/Téléphone: 613-759-1638
} Facsimile/Télécopieur: 613-759-1701
}
} Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
}
} -----Original Message-----
} X-from: TrogadisJ-at-smh.toronto.on.ca [mailto:TrogadisJ-at-smh.toronto.on.ca]
} Sent: Thursday, October 27, 2005 5:12 PM
} To: Yang, Ann-Fook
} Subject: [Microscopy] heating live cells
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Fellow Microscopists
}
} We are looking for a device for maintaining a constant temperature
} inside a plexiglass incubator which is installed around the stage of an
} inverted microscope. Volume around 2 cu. ft.
}
} We now have an external heating unit with temperature control that
} blows warm air into the incubator, however the air then flows out
} through small openings and it is difficult to regulate the CO2 levels
} because the air is constantly changing. Besides, the warm air results in
} fluid evaporation.
}
} Ideally, I'm looking for a radiant source of heat to place inside the
} incubator - no light bulbs, for obvious reasons. We do have a thermistor
} to regulate the temperature into which the device could be plugged.
}
} Any suggestions?
} Thank you
} Judy
}
} Judy Trogadis
} Bio-Imaging Coordinator
} St. Michael's Hospital, 7Queen
} 30 Bond St.
} Toronto, ON M5B 1W8
} Canada
} ph: 416-864-6060 x6337
} pager: 416-685-9219
} fax: 416-864-6043
} trogadisj-at-smh.toronto.on.ca
}
}
} ==============================Original Headers==============================
} 7, 19 -- From trogadisj-at-smh.toronto.on.ca Thu Oct 27 16:09:45 2005
} 7, 19 -- Received: from smh.toronto.on.ca (mail.smh.toronto.on.ca [199.71.175.103])
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From: frank.karl-at-degussa.com
Date: Fri, 28 Oct 2005 09:22:32 -0500
Subject: [Microscopy] LD vs 'regular' LM objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jim,

There's also an inexpensive package that Thierry Epicier built, based on
the SHRLI code I wrote waaay back ("Computed crystal structure images
for high resolution electron microscopy", M.A. O'Keefe, P.R. Buseck and
S. Iijima, Nature 274 (1978) 322-324).

A freeware version of Thierry's image and diffraction package can be
found at:
http://www.amc.anl.gov/ANLSoftwareLibrary/02-MMSLib/HREM/shrli/readme.txt

Alternatively, see the Listserver archives -- go to
http://www.microscopy.com/cgi-bin/ReadPrintEmailHTML.pl?filename=9411.txt
and do a find (ctrl-F) for SHRLI.

I haven't worked in this field for quite a while and I won't have access
to a working copy for the foreseeable future.

Mike
----- Original Message -----
X-from: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}





I don't want to start a flame war, I don't wish to lose my access to this
list server......

Your statement is only part of the story. Image quality is also affected
by the degree of correction built into the objective. An achromatic
objective may have corrections for one color spherical aberration and two
colors chromatic aberration. An apochromatic would have corrections for
two color spherical aberration and three colors chromatic aberration. I
will not even attempt to fit in flat fields, fluorites, semifluorites and
the old fashion gem lens. With that we still have a series of corrections
for comma, flare, barrel and goodness knows what else.

So, Given two objectives of the same quality and correction, I would agree
with you, bigger NA means more potential resolving power up to the NA of
the substage condenser as limited by the refractive index of air. (Now
we're into oil immersion systems!) Remember we're are talking about an
optical system of condenser and objective.

I tell everyone about examining diatoms with two different objectives, one
from company N, the other from company Z (maybe 30 years ago). The two
objectives were the same magnification and had roughly the same NA, but
quality was significantly different. The Z objective made diatom structure
features jump out at me. With the N objective I could find the features
but I had to hunt for them.

There is more to examining images than resolving power.....

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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phillipst-at-missour
i.edu To: frank.karl-at-degussa.com
cc:
10/28/2005 09:23 Subject: [Microscopy] Re: LD vs 'regular' LM objectives
AM
Please respond to
phillipst








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The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


The quality of the image and its ability to capture light (i.e., brightness

of fluorescence images) is determined by the numerical aperture (NA) and
the magnification of the objective. Long working distance objectives
generally have a lower NA so they produce lower resolution and less bright
fluorescent images than standard working distance objectives with similar
magnifications but higher NA's. For a given magnification, the higher the
NA, the brighter and sharper the image. For a given NA, the lower the
magnification, the brighter the image but I think you need to know the
actual NA and mag to predict the effect on resolution.

At 07:07 PM 10/27/05, you wrote:



} ----------------------------------------------------------------------------

} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

}
} Hi
}
} Anyone like to offer an opinion of the differences in image quality
between
} LD and standard objective lenses for light microscopy including
} fluorescence?
}
} We have a 'hybrid' scope, some LD, some standard objectives and are trying
} to decide which kind to add to go to make them all the same.
}
} Pretty general question, I know, but give it a shot if you have some
ideas.
}
} Thanks
}
} Jon
}
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} C230 Earth & Marine Science
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
} ==============================Original
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Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: keith.morris-at-ucl.ac.uk
Date: Fri, 28 Oct 2005 10:39:05 -0500
Subject: [Microscopy] Fw: heating live cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jon,

We only have one Zeiss 'air' LD plan neofluar - a 63x. However it's image
quality is noticeably poorer than our Zeiss oil immersion 63x Plan
Apochromat's, using confocal and standard fluorescence imaging
configurations. The LD objective was over £1,000 cheaper - we use it for
both epi-fluorescence and Ph transmission.

Our LD obviously scores if you want to image something 1mm into the sample
though owing to its very long working distance (WD 1.2 to 2.2 mm). In
comparison our Plan Apochromat high power oil objectives (WD = 0.19 mm) only
just get into the cells stuck onto the coverslip (e.g. using Mattek dishes)
and we can't see anything at all with a slightly raised coverslip on slides.

On our Leica SP2 AOBS confocal system, our 'blue' Leica 20x HCL PL APO
water/glycerol/oil objective has very good image quality and a slightly
bigger WD with water compared to oil immersion (free WD = 0.26 mm water or
0.17 mm oil). However water immersion is a pain with the inverted
microscopes we use.

Our local Zeiss rep is always happy to lend us objectives for appraisal,
which is extremely useful.

Regards

Keith

PS. Years ago at a meeting I heard that higher NA increases the resolution,
but lowering the NA (assuming you have a variable NA collar on your
objective) improves the contrast, which might be more useful in some cases.
It seems to work with the one variable NA objective we have.

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


----- Original Message -----
X-from: {jmkrupp-at-cats.ucsc.edu}
To: {keith.morris-at-ucl.ac.uk}
Sent: Friday, October 28, 2005 1:11 AM

Hi Judy,

I use fixed temperature 'convection enclosure heaters' (see RSWWW link).
They are really designed for eliminating condensation in outside
enclosures, but they simply plug in and then go to a set surface
temperature with no thermostat control. They are designed to stay on
permanently and have overheat & thermal fuse protection etc..

I have two 30w enclosure heaters to supply additional heat inside a large
Perspex microscope incubator (similar to www.solent-scientific.co.uk ones).
They are fitted into little aluminium stands our workshop made for them.
With the thermostatically controlled 'air blower' off & the enclosure
heaters on, the incubator stays around 32oC ready to be quickly heated when
the Zeiss/PeCon CTI/3700 'air blow temp' controllers are switched on. I do
put bubble wrap on the back, sides and top of the incubator, to reduce heat
loss to the room.

See them at
http://www.rswww.com and search for item 224-492 .

They may suit. They are cheap and reasonably compact.

The 30W ones get to around 57oC surface temp when left on, and should be
placed a few cm's from cables etc... The stands we built for them also
shield the side surfaces from fingers as otherwise they are hot to touch
(the higher the wattage the hotter they get).

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


----- Original Message -----
} From: {TrogadisJ-at-smh.toronto.on.ca}
} To: {keith.morris-at-ucl.ac.uk}
} Sent: Thursday, October 27, 2005 10:16 PM
} Subject: [Microscopy] heating live cells
}
}
} }
} }
} }
} } ----------------------------------------------------------------------------
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} }
} } Fellow Microscopists
} }
} } We are looking for a device for maintaining a constant temperature
} } inside a plexiglass incubator which is installed around the stage of an
} } inverted microscope. Volume around 2 cu. ft.
} }
} } We now have an external heating unit with temperature control that
} } blows warm air into the incubator, however the air then flows out
} } through small openings and it is difficult to regulate the CO2 levels
} } because the air is constantly changing. Besides, the warm air results in
} } fluid evaporation.
} }
} } Ideally, I'm looking for a radiant source of heat to place inside the
} } incubator - no light bulbs, for obvious reasons. We do have a thermistor
} } to regulate the temperature into which the device could be plugged.
} }
} } Any suggestions?
} } Thank you
} } Judy
} }
} } Judy Trogadis
} } Bio-Imaging Coordinator
} } St. Michael's Hospital, 7Queen
} } 30 Bond St.
} } Toronto, ON M5B 1W8
} } Canada
} } ph: 416-864-6060 x6337
} } pager: 416-685-9219
} } fax: 416-864-6043
} } trogadisj-at-smh.toronto.on.ca
} }
} }
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From: nairvinods-at-gmail.com
Date: Fri, 28 Oct 2005 12:33:28 -0500
Subject: [Microscopy] Immuno TEM ....GFP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Randy,
I am a grad student at NMSU and have been working some immunolabelling. We
are looking at localization of GFP labelled bacteria in squid tissue. So far
I have had luck immunolbelling GFP transformed bacteria with colloidal gold.
I am using LR white for embedding. I haven't encountered any problems thus
far with immunolabelling.
The only problem I have is that I have a huge tissue and I need to section
transversly thru the entire tissue. This leaves a lot of wrinkles behind
after the whole process of immunlabeling which does not look real good after
scanning the negatives. The sections are very thin to withstand stretching
using chloroform and other resins seem to affect immunolabelling.
This is where I could use some help.
Would it be ethical to try and iron out the wrinkles using any software.
I would greatly appreciate any suggestions and or solutions to iron out those
wrinkles. Unfortunately re embedding is not an option as we do not have any
more tissue.
Desperate to graduate :)
Vinod


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2, 23 -- From: Vinod Nair {nairvinods-at-gmail.com}
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2, 23 -- Subject: Immuno TEM ....GFP
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From: sghoshro-at-NMSU.Edu
Date: Fri, 28 Oct 2005 12:44:04 -0500
Subject: [Microscopy] Re: Immuno EM---GFP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,

A graduate student in the lab routinely uses gold conjugated anti-GFP secondary
antibody to label GFP in LR White embedded tissue. It is post section labeling
and works just fine. You can buy the secondary ab from I believe Jackson
Immunoresearch Lab.

Can you Fed Ex me some left overs from your dinner party?

Soumitra


Quoting TindallR-at-missouri.edu:

}
}
}
} ----------------------------------------------------------------------------
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} Dear Listers,
}
} I'm curious to see if anyone has any words of wisdom about
} immunostaining of GFP in general. I'd be interested in experiences
} relating to DAB-HRP labeling and/or immunogold, both pre- and
} post-embedding. Cryosections, too! (There, that ought to keep you
} busy.)
}
} More specifically, are there any danger spots in the fixation or
} embedding processes that could negatively affect the labeling process,
} or even destroy the protein itself?
}
} I know it's one of those overly broad questions, but I am mainly
} interested in how, if at all, labeling GFP might be different that
} labeling other proteins. I've not had much luck going through our
} library on finding GFP-specific material, and I'm still Googling away.
}
} By the way, you're all invited to dinner at my place tonight by way of
} thanks. My Honduran in-laws are in town and the food is good and
} plentiful and the music is Latin. Yes, people CAN cook and dance at the
} same time.
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
}
}
}
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Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office)
505-646-3283 (lab)
Fax: 505-646-3282
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml

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From: opmills-at-mtu.edu
Date: Fri, 28 Oct 2005 14:16:22 -0500
Subject: [Microscopy] Magnetic card readers for instrument access

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I've been alerted that funding may be available for the installation
of magnetic strip card readers to tracking instrument use in my
facility. Apparently they are being used successfully for this
purpose in a university micro-fabrication facility in our state. If
they work, perhaps it would simplify the accounting involved in
monthly invoicing. However, I have a couple of concerns about using
them in our facility that I can't work out. Maybe you can help.

Do you install the reader on the instrument itself, or on the doorway
of the lab. This is the standard way of installing readers at my
university. There is a problem when they are used on a lab door when
there are several instruments inside.

Can readers be interlocked into the instruments to prevent use until
a card is swiped? Has anyone done that? How?

If I use a swipe style reader, we could probably get compliance when
a user gets started. However, we'd have to get them to swipe again
at the end of their use to capture the elapsed time (which is what we
really want!). If they don't swipe again at the end of their
session, I'll have to spend more time determining how much time they
used.

Have any of you used a card reader like the ones on ATM machines that
actually "keep" your card until the transaction is completed? A
reader like this might solve the problem above.

There is a problem for automated instruments (like microprobes) where
a sample run may extend into the early morning hours. Since no one
will be there to swipe at the end, how could we accurately track use
in those situations?

I appreciate any input you can offer. Thanks!

Owen

Owen P. Mills
Director, Materials Characterization and Fabrication Facilities
Electron Optics Engineer,
Applied Chemical & Morphological Analysis Laboratory
Materials Science & Engineering
Michigan Technological University
Rm 512 M&M Bldg.
Houghton, MI 49931
PH 906-369-1875
FAX 906-487-2934
mailto:opmills-at-mtu.edu
http://www.mm.mtu.edu/~opmills




Owen P. Mills
Director, Materials Characterization and Fabrication Facilities
Electron Optics Engineer,
Applied Chemical & Morphological Analysis Laboratory
Materials Science & Engineering
Michigan Technological University
Rm 512 M&M Bldg.
Houghton, MI 49931
PH 906-369-1875
FAX 906-487-2934
mailto:opmills-at-mtu.edu
http://www.mm.mtu.edu/~opmills




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From: gcc-at-couger.com
Date: Fri, 28 Oct 2005 14:20:37 -0500
Subject: [Microscopy] heating live cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





} Fellow Microscopists
}
} We are looking for a device for maintaining a constant temperature
} inside a plexiglass incubator which is installed around the stage of an
} inverted microscope. Volume around 2 cu. ft.
}
} We now have an external heating unit with temperature control that
} blows warm air into the incubator, however the air then flows out
} through small openings and it is difficult to regulate the CO2 levels
} because the air is constantly changing. Besides, the warm air results in
} fluid evaporation.
}
} Ideally, I'm looking for a radiant source of heat to place inside the
} incubator - no light bulbs, for obvious reasons. We do have a thermistor
} to regulate the temperature into which the device could be plugged.
}
} Any suggestions?
} Thank you
} Judy
}
} Judy Trogadis

Hi Judy,

If you need an out of the box solution a look for PID temperature control
such as http://www.watlow.com/products/controllers/ and for that small
chamber I would use several Caddock resistors
http://www.caddock.com/Online_catalog/current_sense/current_sense.html
over the bottom to evenly contort the heat using a 6 or 12 volt
transformer that you should have laying around.---

For more complex solutions here are some options.

I help an entomologist solve the CO2 problem in moving air as show in
Perritt,D.W., Baker, R.R., Couger, G.
Computer lactometer system for studying behavioral responses of ticks to
carbon dioxide.
Journal of medical entomology. (ABBREV TITLE = J Med Entomol) May 1993. v.
30 (3)

I believe the specifics on how to build the apparatus and computer
program are in the paper. If the particulars an the apparatus aren't in
the paper it requires a dual stage regulator for the CO2, $25 dollar 12
volt solenoid valve and a one dollar FET and uses a printer port of MS DOS
computer to run it. It is an open loop system that is set by trial and
error but it can set the CO2 concentration in air very closely..

I would have made a more outburst solution but it was done for another
department and they needed a quick fix and my boss was on my bake about
helping others outside the department.

Depending on what kind or computer programing resources you have
available. On the crude end a light Bulb and a very small fan blowing over
it does a very good job raising the temperature above the ambient
temperature with very little over shoot if the bulb is sized right. The
fan needs to come on with the light bulb and stay on until it is cooled
down to near the temperature of the chamber. I have used power resistors
such as these
http://www.caddock.com/Online_catalog/current_sense/current_sense.html as
heater as well and you can get them in any power dissipation rang and wide
range of resistance to use any voltage to make a heater.

If you have no low level computer programming experience available the
Tiny 2131 and Plug-in-Bord from http://www.newmicros.com/ using FORT is as
easy to get started with as any and they have enough example program to
almost make it work.

FORTH is difficult language for big projects but with everything being on
board and always ready to go it is very nice for small ones.

I have held the temperature of 1.5 cubic foot box with in 2 degrees with
the logic of if the temperature is over 150f turn it off and let the fan
run for one minute if the temperature is below 149 turn on the light and
wait 10 seconds to turn on the fan. Each of 8 faces of the box had
different insulation.

If you need an out of the box solution a look for PID temperature control
such as http://www.watlow.com/products/controllers/ and for that small
chamber I would use several Caddock resistors
http://www.caddock.com/Online_catalog/current_sense/current_sense.html
over the bottom to evenly control the heat using a 6 or 12 volt
transformer that you should have laying around from the old days.

Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org


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From: phillipst-at-missouri.edu
Date: Fri, 28 Oct 2005 14:51:03 -0500
Subject: [Microscopy] LD vs 'regular' LM objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Indeed, I agree with all of Frank's comments. My earlier answer was too
short and Frank addressed several issues I didn't mention. Furthermore,
when discussing fluorescence, one has to worry about how well they transmit
the wavelength one is interested in. For example, a highly corrected
objective may have too much glass or the wrong type of glass to adequately
pass UV wavelengths. The ability to pass NIR wavelengths is important in
selecting objectives for multiphoton confocal. I also fully agree that
objective quality can vary between manufacturers but a comparison between
brands N and Z 30 years ago is not especially germane anymore since all the
major manufacturers have really changed how they make their objectives in
the last 30 years.

At 09:21 AM 10/28/05, you wrote:




} I don't want to start a flame war, I don't wish to lose my access to this
} list server......
}
} Your statement is only part of the story. Image quality is also affected
} by the degree of correction built into the objective. An achromatic
} objective may have corrections for one color spherical aberration and two
} colors chromatic aberration. An apochromatic would have corrections for
} two color spherical aberration and three colors chromatic aberration. I
} will not even attempt to fit in flat fields, fluorites, semifluorites and
} the old fashion gem lens. With that we still have a series of corrections
} for comma, flare, barrel and goodness knows what else.
}
} So, Given two objectives of the same quality and correction, I would agree
} with you, bigger NA means more potential resolving power up to the NA of
} the substage condenser as limited by the refractive index of air. (Now
} we're into oil immersion systems!) Remember we're are talking about an
} optical system of condenser and objective.
}
} I tell everyone about examining diatoms with two different objectives, one
} from company N, the other from company Z (maybe 30 years ago). The two
} objectives were the same magnification and had roughly the same NA, but
} quality was significantly different. The Z objective made diatom structure
} features jump out at me. With the N objective I could find the features
} but I had to hunt for them.
}
} There is more to examining images than resolving power.....
}
} Frank Karl
} Degussa Corporation
} Akron Technical Center
} 3500 Embassy Parkway
} Suite 100
} Akron, Ohio 44333
}
}
} 330-668-2235 Ext. 238
}
}
} This e-mail transmission, and any documents, files or previous e-mail
} messages attached to it may contain information that is confidential or
} legally privileged. If you are not the intended recipient, or a person
} responsible for delivering it to the intended recipient, you are hereby
} notified that you must not read this transmission and that any disclosure,
} copying, printing, distribution or use of any of the information contained
} in or attached to this transmission is STRICTLY PROHIBITED. If you have
} received this transmission in error, please immediately notify the sender
} by telephone or return e-mail and delete the original transmission and its
} attachments without reading or saving in any manner. Thank you.
}
}
}
}
} phillipst-at-missour
}
} i.edu To:
} frank.karl-at-degussa.com
} cc:
}
} 10/28/2005 09:23 Subject: [Microscopy] Re:
} LD vs 'regular' LM objectives
} AM
}
} Please respond
} to
}
} phillipst
}
}
}
}
}
}
}
}
}
}
} ----------------------------------------------------------------------------
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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

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Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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10, 23 -- To: frank.karl-at-degussa.com
10, 23 -- From: Tom Phillips {phillipst-at-missouri.edu}
10, 23 -- Subject: Re: [Microscopy] Re: LD vs 'regular' LM objectives
10, 23 -- Cc: Microscopy-at-msa.microscopy.com
10, 23 -- In-Reply-To: {OF9F169D40.363DEC64-ON852570A8.004CD83A-852570A8.004EDCA0-at-
10, 23 -- degussa.com}
10, 23 -- References: {200510281323.j9SDNSx2014886-at-ns.microscopy.com}
10, 23 -- {OF9F169D40.363DEC64-ON852570A8.004CD83A-852570A8.004EDCA0-at-degussa.com}
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==============================End of - Headers==============================




From: TrogadisJ-at-smh.toronto.on.ca
Date: Fri, 28 Oct 2005 15:03:08 -0500
Subject: [Microscopy] live cell heating device

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To the wonderful Microscopy Listserv:

Thank you for the many useful replies with suggestions on regulating
the temperature in our incubator. Of course, off-the-shelf products are
great, however, it was astonishing yet reassuring to know that
individuals are still willing to spend the time to create novel ways of
achieving the same results.

Gratefully yours,
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8
Canada
ph: 416-864-6060 x6337


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From: David.Griffiths-at-veths.no
Date: Fri, 28 Oct 2005 16:33:18 -0500
Subject: [Microscopy] LM_Microtomes_HPMA_Methacrylates_Recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listmembers,
I need to order a new microtome primarily for sectioning methacrylate-embedded tissue for light microscopy. The choice will probably be between the Microm HM-355C and an RMC MT-990, with possibly a Leica RM2255 also being a candidate. I know almost nothing about the Microm, and absolutely nothing about the RMC. If any of you have used either of these products or have any firm opinions of them I would really like to hear from you. This probably isn't of great interest to most people, so perhaps offline to me at david.griffiths-at-veths.no is best.

Thankyou,
David Griffiths

David Griffiths
Section of Anatomy and Pathology
Norwegian School of Veterinary Science
P. O. Box 8146 Dep
N-0033 Oslo
Norway
E-mail: david.griffiths-at-veths.no

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From: jmastrangelo-at-ulbi.com
Date: Fri, 28 Oct 2005 17:45:22 -0500
Subject: [Microscopy] viaWWW: Mn / F analysis with EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jmastrangelo-at-ulbi.com) from http://www.microscopy.com/MLFormMail.html on Friday, October 28, 2005 at 08:54:33
---------------------------------------------------------------------------

Email: jmastrangelo-at-ulbi.com
Name: Joseph Mastrangelo

Organization: Ultralife Batteries

Title-Subject: [Filtered] Mn / F resolution with EDS

Question: Good morning everyone,

We have recently purchased an SEM/EDS system for the purpose of materials qualification. The system seems to be working fine, but we are having some difficulty when attempting to analyze fluorine in samples that contain high amounts of manganese. We assume this difficulty is due to the ROI overlap for Mn and F. We do realize that the EDS system is semi-quantitative at best, but the overlap seems to be causing the Fluorine content to be magnified by a factor of 3 or 4 in the analysis. Just wondering if anyone out there has had any similar experiences, and whether there are any "tricks of the trade" for dealing with this.

Thanks in advance for your input,

Joe Mastrangelo
Chemical Lab Technician
Ultralife Batteries
Newark, NY
jmastrangelo-at-ulbi.com

---------------------------------------------------------------------------

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9, 12 -- From zaluzec-at-microscopy.com Fri Oct 28 17:45:22 2005
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9, 12 -- Subject: viaWWW: Mn / F analysis with EDS
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From: gcc-at-couger.com
Date: Fri, 28 Oct 2005 21:26:52 -0500
Subject: [Microscopy] Re: Magnetic card readers for instrument access

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Owen,

To get that level of compliance you would have to implant RFID chips in
the users hands and only activate the scope when it senses and ID tag in
the area and register the user.

You should be able to get a satisfactory level of compliance from
professionals and student with an ID chip in a bracelet, ring. watch band,
active ID badge or card in a passive system. The RFID sensor registers,
records and turns on the instrument if the user has privileges for it from
who ever's RFID is in the capture area of the scope. Here is one example I
found with Google search "RFID card" SDK
http://www.geometrix.com/solutions/access.html

Be careful in this area you are very close to the system being put in
track cattle from conception to the fork so be careful how you present the
idea on bad joke can send it down the tubes.


I expect the RFID solution would be more expensive than the card swipe but
the RFID should work almost every time logging users out and catching the
change of users with in a few minuets of the actual change. I expect you
will be lucky to get 40% log off compliance with a card swipe. I know I
would forget a good part of the time.

I am an inactive part owner of a company that develops this kind of
solution as well as microscope hobbyist. We have done work in this area
but have not done any projects.

Gordon Couger
DataLink Systems
www.rfdata.net




opmills-at-mtu.edu wrote:

}
} Hi,
}
} I've been alerted that funding may be available for the installation
} of magnetic strip card readers to tracking instrument use in my
} facility. Apparently they are being used successfully for this
} purpose in a university micro-fabrication facility in our state. If
} they work, perhaps it would simplify the accounting involved in
} monthly invoicing. However, I have a couple of concerns about using
} them in our facility that I can't work out. Maybe you can help.
}
} Do you install the reader on the instrument itself, or on the doorway
} of the lab. This is the standard way of installing readers at my
} university. There is a problem when they are used on a lab door when
} there are several instruments inside.
}
} Can readers be interlocked into the instruments to prevent use until
} a card is swiped? Has anyone done that? How?
}
} If I use a swipe style reader, we could probably get compliance when
} a user gets started. However, we'd have to get them to swipe again
} at the end of their use to capture the elapsed time (which is what we
} really want!). If they don't swipe again at the end of their
} session, I'll have to spend more time determining how much time they
} used.
}
} Have any of you used a card reader like the ones on ATM machines that
} actually "keep" your card until the transaction is completed? A
} reader like this might solve the problem above.
}
} There is a problem for automated instruments (like microprobes) where
} a sample run may extend into the early morning hours. Since no one
} will be there to swipe at the end, how could we accurately track use
} in those situations?
}
} I appreciate any input you can offer. Thanks!
}
} Owen
}
} Owen P. Mills
} Director, Materials Characterization and Fabrication Facilities
} Electron Optics Engineer,
} Applied Chemical & Morphological Analysis Laboratory
} Materials Science & Engineering
} Michigan Technological University
} Rm 512 M&M Bldg.
} Houghton, MI 49931
} PH 906-369-1875
} FAX 906-487-2934
} mailto:opmills-at-mtu.edu
} http://www.mm.mtu.edu/~opmills
}
}
}
}
} Owen P. Mills
} Director, Materials Characterization and Fabrication Facilities
} Electron Optics Engineer,
} Applied Chemical & Morphological Analysis Laboratory
} Materials Science & Engineering
} Michigan Technological University
} Rm 512 M&M Bldg.
} Houghton, MI 49931
} PH 906-369-1875
} FAX 906-487-2934
} mailto:opmills-at-mtu.edu
} http://www.mm.mtu.edu/~opmills
}

}


==============================Original Headers==============================
15, 21 -- From gcc-at-couger.com Fri Oct 28 21:26:52 2005
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15, 21 -- Received: from [127.0.0.1] (really [68.12.242.13]) by centrmmtao06.cox.net
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15, 21 -- id {20051029022609.BZKD24602.centrmmtao06.cox.net-at-[127.0.0.1]} ;
15, 21 -- Fri, 28 Oct 2005 22:26:09 -0400
15, 21 -- Message-ID: {4362DDEA.2000200-at-couger.com}
15, 21 -- Date: Fri, 28 Oct 2005 21:26:50 -0500
15, 21 -- From: Gordon Couger {gcc-at-couger.com}
15, 21 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716)
15, 21 -- X-Accept-Language: en-us, en
15, 21 -- MIME-Version: 1.0
15, 21 -- To: opmills-at-mtu.edu,
15, 21 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com}
15, 21 -- Subject: Re: [Microscopy] Magnetic card readers for instrument access
15, 21 -- References: {200510281920.j9SJKCoh002237-at-ns.microscopy.com}
15, 21 -- In-Reply-To: {200510281920.j9SJKCoh002237-at-ns.microscopy.com}
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From: gcc-at-couger.com
Date: Sat, 29 Oct 2005 00:40:24 -0500
Subject: [Microscopy] LD vs 'regular' LM objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hi Jon,
}
} We only have one Zeiss 'air' LD plan neofluar - a 63x. However it's image
} quality is noticeably poorer than our Zeiss oil immersion 63x Plan
} Apochromat's, using confocal and standard fluorescence imaging
} configurations. The LD objective was over £1,000 cheaper - we use it for
} both epi-fluorescence and Ph transmission.
}
} Our LD obviously scores if you want to image something 1mm into the sample
} though owing to its very long working distance (WD 1.2 to 2.2 mm). In
} comparison our Plan Apochromat high power oil objectives (WD = 0.19 mm)
only
} just get into the cells stuck onto the coverslip (e.g. using Mattek
dishes)
} and we can't see anything at all with a slightly raised coverslip on
slides.
}
} On our Leica SP2 AOBS confocal system, our 'blue' Leica 20x HCL PL APO
} water/glycerol/oil objective has very good image quality and a slightly
} bigger WD with water compared to oil immersion (free WD = 0.26 mm water or
} 0.17 mm oil). However water immersion is a pain with the inverted
} microscopes we use.
}
} Our local Zeiss rep is always happy to lend us objectives for appraisal,
} which is extremely useful.
}
} Regards
}
} Keith
}
} PS. Years ago at a meeting I heard that higher NA increases the
resolution,
} but lowering the NA (assuming you have a variable NA collar on your
} objective) improves the contrast, which might be more useful in some
cases.
} It seems to work with the one variable NA objective we have.
}
Keith,

Your pretty much right but the n.a. included the coder and the lower the
n.a. the larger the depth of field. In my mind I believe that is stating
the same thing in two different ways. But its ben 44 years since I had
physics.

I have a Leitz 63x .83 n.a. with a variable aperture it is the companion
to their dry dark field condensers and everything you say about the effect
of n.a. is pretty much on the money. The iris can be closed to the point
on the Leitz 63x that n.a. is so small that the image degradation due to
detraction on the edge of the blades of the iris is noticeable.

Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org





==============================Original Headers==============================
10, 22 -- From gcc-at-couger.com Sat Oct 29 00:40:24 2005
10, 22 -- Received: from centrmmtao06.cox.net (centrmmtao06.cox.net [70.168.83.78])
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10, 22 -- id {20051029053941.DKQZ24602.centrmmtao06.cox.net-at-[127.0.0.1]} ;
10, 22 -- Sat, 29 Oct 2005 01:39:41 -0400
10, 22 -- Message-ID: {43630B4A.4040500-at-couger.com}
10, 22 -- Date: Sat, 29 Oct 2005 00:40:26 -0500
10, 22 -- From: Gordon Couger {gcc-at-couger.com}
10, 22 -- User-Agent: Mozilla Thunderbird 1.0.6 (Windows/20050716)
10, 22 -- X-Accept-Language: en-us, en
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10, 22 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com}
10, 22 -- Subject: Re: [Microscopy] Re: LD vs 'regular' LM objectives
10, 22 -- References: {200510281454.j9SEsPQH028772-at-ns.microscopy.com}
10, 22 -- In-Reply-To: {200510281454.j9SEsPQH028772-at-ns.microscopy.com}
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From: Rosemary.White-at-csiro.au
Date: Sat, 29 Oct 2005 02:25:16 -0500
Subject: [Microscopy] Re: LD vs 'regular' LM objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Keith et al.,

We have 4 dipping objectives (no coverslip needed) with about 2 mm WD, as
well as high NA, short WD water immersion objectives. We use the dipping
objectives on the upright microscope when we need to do microinjection or
change incubation solution or look at large, odd-shaped specimens, etc. I
am impressed at how good they are. The 63x dipping objective, with NA 0.9,
is very bright (for fluorescence) and quite good resolution, though you can
clearly see the superior resolution and brightness of the NA 1.2 water
immersion objective. Since the latter cost about twice as much as the
former, you'd want to see a difference in performance.....

Rosemary

Rosemary White rosemary.white-at-csiro.au
CSIRO Plant Industry ph. 02-6246 5475
GPO Box 1600 mob. 0402 835 973
Canberra, ACT 2601 fax. 02-6246 5334
Australia


} From: gcc-at-couger.com
} Reply-To: gcc-at-couger.com
} Date: Sat, 29 Oct 2005 00:44:29 -0500
} To: rosemary.white-at-csiro.au
} Subject: [Microscopy] LD vs 'regular' LM objectives
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
}
} } Hi Jon,
} }
} } We only have one Zeiss 'air' LD plan neofluar - a 63x. However it's image
} } quality is noticeably poorer than our Zeiss oil immersion 63x Plan
} } Apochromat's, using confocal and standard fluorescence imaging
} } configurations. The LD objective was over £1,000 cheaper - we use it for
} } both epi-fluorescence and Ph transmission.
} }
} } Our LD obviously scores if you want to image something 1mm into the sample
} } though owing to its very long working distance (WD 1.2 to 2.2 mm). In
} } comparison our Plan Apochromat high power oil objectives (WD = 0.19 mm)
} only
} } just get into the cells stuck onto the coverslip (e.g. using Mattek
} dishes)
} } and we can't see anything at all with a slightly raised coverslip on
} slides.
} }
} } On our Leica SP2 AOBS confocal system, our 'blue' Leica 20x HCL PL APO
} } water/glycerol/oil objective has very good image quality and a slightly
} } bigger WD with water compared to oil immersion (free WD = 0.26 mm water or
} } 0.17 mm oil). However water immersion is a pain with the inverted
} } microscopes we use.
} }
} } Our local Zeiss rep is always happy to lend us objectives for appraisal,
} } which is extremely useful.
} }
} } Regards
} }
} } Keith
} }
} } PS. Years ago at a meeting I heard that higher NA increases the
} resolution,
} } but lowering the NA (assuming you have a variable NA collar on your
} } objective) improves the contrast, which might be more useful in some
} cases.
} } It seems to work with the one variable NA objective we have.
} }
} Keith,
}
} Your pretty much right but the n.a. included the coder and the lower the
} n.a. the larger the depth of field. In my mind I believe that is stating
} the same thing in two different ways. But its ben 44 years since I had
} physics.
}
} I have a Leitz 63x .83 n.a. with a variable aperture it is the companion
} to their dry dark field condensers and everything you say about the effect
} of n.a. is pretty much on the money. The iris can be closed to the point
} on the Leitz 63x that n.a. is so small that the image degradation due to
} detraction on the edge of the blades of the iris is noticeable.
}
} Gordon
} Gordon Couger
}
} I collect links on information related to light microscopes.
} www.couger.com/microscope/links/gclinks.html
} Please forward anything you think might be useful to others.
} Microscope Documentation is at www.science-info.org
}
}
}
}
}
} ==============================Original Headers==============================
} 10, 22 -- From gcc-at-couger.com Sat Oct 29 00:40:24 2005
} 10, 22 -- Received: from centrmmtao06.cox.net (centrmmtao06.cox.net
} [70.168.83.78])
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} -0500
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} centrmmtao06.cox.net
} 10, 22 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with
} ESMTP
} 10, 22 -- id
} {20051029053941.DKQZ24602.centrmmtao06.cox.net-at-[127.0.0.1]} ;
} 10, 22 -- Sat, 29 Oct 2005 01:39:41 -0400
} 10, 22 -- Message-ID: {43630B4A.4040500-at-couger.com}
} 10, 22 -- Date: Sat, 29 Oct 2005 00:40:26 -0500
} 10, 22 -- From: Gordon Couger {gcc-at-couger.com}
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} 10, 22 -- "microscopy-at-msa.microscopy.com"
} {microscopy-at-msa.microscopy.com}
} 10, 22 -- Subject: Re: [Microscopy] Re: LD vs 'regular' LM objectives
} 10, 22 -- References: {200510281454.j9SEsPQH028772-at-ns.microscopy.com}
} 10, 22 -- In-Reply-To: {200510281454.j9SEsPQH028772-at-ns.microscopy.com}
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}


==============================Original Headers==============================
7, 22 -- From Rosemary.White-at-csiro.au Sat Oct 29 02:25:15 2005
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From: zaluzec-at-microscopy.com
Date: Sat, 29 Oct 2005 12:19:18 -0500
Subject: [Microscopy] Microscopy & Microanalysis 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

The WWW site for the Microscopy & Microanalysis 2006 Meeting
in Chicago July 30-August 3 has been recently updated with the
full meeting details.

http://mm2006.microscopy.org

Please feel free to visit this site at your convenience
and to share this information with colleagues and students who may not
be members of this Listserver community.

While a few of the on-line registration/submission pages are still in the
process of being established, the all of the information concerning the meeting
as well as Pre-Meeting Congress and Sunday Short Courses is now
on-line.

A hard copy of the Call for Papers is currently in press and will be mailed shortly to
all sponsoring society members as well as recent meeting participants.

Hope to see you (all) in Chicago, as by some strange coincidence it appears I will
also be there.

Nestor J. Zaluzec
Your Friendly Neighborhood SysOp
&
MM2006 Local Arrangement Committee Co-Chair


==============================Original Headers==============================
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9, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}
9, 11 -- Subject: Microscopy & Microanalysis 2006
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From: eggert-at-mikroanalytik.de
Date: Mon, 31 Oct 2005 00:24:09 -0600
Subject: [Microscopy] Re: viaWWW: Mn / F analysis with EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Joe,

the overlap of F between several Mn-L lines is very strong and a real
challenge for each deconvolution procedure. The deconvolution software
must know all line positions very well and the distortions of lines
(detector dependance). But the major problem is not to deconvolute the
net count rates of all lines in this spectra region, but the strong
absorption of F in Manganese due to the Mn-L3 and L2 absorption jumps.
This should be the main problem for quantitative analysis, not only for
an EDS.

Look to some numbers: If the F is emitted in 0.1 micron depth of
specimen, only about 19 per cent of X-rays of 677 eV (F-Ka) are able to
leave the specimen (the opposite is absorbed). Nothing is coming from
0.3 micron depth. Only a little change in energy to a lower X-ray energy
(630 eV) and 55% are coming out from 0.3 micron and 82 per cent from 0.1.

This is the real problem, your quantitative software is faced to. The
absorption is extremly strong. Therefore the errors and fluctuations you
must expect in quantitative results are much higher than with other
analytical situations. Finally the absolute deviations of your results
depends of the mass absorption coefficients your software is using.
These coefficients are badly well-known around absorption edges and
still more badly in low energy regions (below 1 keV). If your system
magnify the Fluorine content with factor 3..4 with samples of high
Manganese contents, the mass absorption coefficient of F in Mn must be
simply too high. But take in mind, the results fluctuations you have to
expect are still present, even if you will able to adjust the
coefficient (due to the always strong absorption).

You can have a look to the line energies, absorption jumps, mass
absorption coefficients (MAC's) and the absorption of X-rays in a given
layer with MA-Table program:

http://www.microanalyst.net/registr.phtml and
http://www.microanalyst.net/manual.html

Best regards

Frank Eggert

jmastrangelo-at-ulbi.com wrote:

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==============================Original Headers==============================
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11, 19 -- Date: Mon, 31 Oct 2005 07:31:26 +0100
11, 19 -- From: Frank Eggert {eggert-at-mikroanalytik.de}
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From: marko-at-wadsworth.org
Date: Mon, 31 Oct 2005 09:38:06 -0600
Subject: [Microscopy] Invitation to organize symposia for M&M2007

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are looking for some great ideas for symposia at M&M2007!

Microscopy & Microanalysis 2007 Meeting

August 6-9, 2007
Broward County Convention Center
Ft. Lauderdale, Florida

Co-sponsored by

The Microscopy Society of America
The Microbeam Analysis Society
The International Metallographic Society

Although we have suggestions for some of the customary symposia, and have
already signed on a small number of organizers, the program is largely open
at this time.

We would like the majority of the proposals to be submitted by the end of
the year. We will start sending out acceptance letters in late December,
and by mid-February we expect the program to be mostly filled.

This timetable is considerably accelerated in comparison with previous
years, and we now require a description of 150-300 words for each proposed
symposia. The description should take the form of those found in the Call
for Papers and Expo of past years; it should be an announcement of the
symposium and an invitation for contributions. The Program Committee will
select symposia based on these descriptions, so that overlap will be
minimized and symposia will complement each other to form a coherent
overall program.

You need not be a member of MSA, MAS, or IMS to propose a symposium,
although we hope that your experience with the M&M meeting will encourage
you to join.

Please send your suggestion (complete with description) directly to the
Program Chair, or to the M&M2007 Co-Chair of your Society.

The M&M2007 website is

http://mm2007.microscopy.org/

The site can be reached by many right now, and contains the same
information as in this email. It will be available to all by the end of
next week. Near the end of the year, accepted symposia will be posted on
the website.

Program contacts:

Mike Marko, Program Chair (marko-at-wadsworth.org)
John Henry Scott, Vice Program Chair (johnhenry.scott-at-nist.gov)
Ed Vincenzi, MAS Program Co-Chair (vicenzi-at-volcano.si.edu)
Steve Dekanich, IMS Program Co-Chair (dekanichsj-at-y12.doe.gov)

Local Arrangments contact:

Lucille Gianuzzi, Local Arrangements Chair (lgiannuzzi-at-feico.com)

Meeting Management contact:

Phillip Ridley, Conference Manager
Bostrom Corp
230 East Ohio, Suite 400
Chicago, IL 60611
Tel: 312-644-0828
Fax: 312-644-8557
Email: pridley-at-bostrom.com





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From: KOCHINST-at-GLOBO.COM
Date: Mon, 31 Oct 2005 20:43:41 -0600
Subject: [Microscopy] viaWWW: first freezing microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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Email: KOCHINST-at-GLOBO.COM
Name: ROBERT KOCH

Organization: KOCH INSTRUMENTOS CIENTÕFICOS LTDA.

Title-Subject: [Filtered] MListserver:

Question: Dear Microscopists,

we would like to know, if someone has information about the
very first freezing microtome.

Thank you

Robert Koch

Koch instrumentos CientÌficos Ltda.
Rua Joaquim Nabuco, 655 S“o Paulo - SP - Brazil
55-11-5092-4622 tel/fax
kochinst-at-globo.com
www.kochinst.com.br

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==============================Original Headers==============================
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From: l.tetley-at-bio.gla.ac.uk
Date: Tue, 1 Nov 2005 06:51:20 -0600
Subject: [Microscopy] 33rd Scottish Microscopy Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



------------------------------------------------------------------------------
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33rd Scottish Microscopy Symposium


Wednesday 9th November 2005, Hunter Halls, University of Glasgow, Glasgow
G12 8QQ, Scotland, UK

Registration - with a 75% discount for students - is via

http://www.gla.ac.uk/ibls/II/em/SMG/smgnew.html

with a programme available at

http://www.gla.ac.uk/ibls/II/em/SMG/programme.pdf


plus a 25 company Trade Exhibition :

http://www.gla.ac.uk/departments/ibls/II/em/SMG/trade.html.


All welcome but registration forms should be FAXed to Will Maxwell at 0141
330 4299 in order to assess numbers for catering. This can be followed by
payment made on arrival.

Dr Laurence Tetley
Division of Infection & Immunity, IBLS,
Integrated Microscopy Facility
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

l.tetley-at-bio.gla.ac.uk
tel 0141 330 4431
FAX 0141 330 3516

Integrated Microscopy
Facility: http://www.gla.ac.uk/Acad/IBLS/II/em/mcb-em.htm
Cryo Microscopy Group: http://www.cryomicroscopygroup.org.uk
Royal Microscopical Society: http://www.rms.org.uk


Dr Laurence Tetley
Division of Infection & Immunity, IBLS,
Integrated Microscopy Facility
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

Tel 0141 330 4431
FAX 0141 330 3516

Integrated Microscopy Facility: http://www.gla.ac.uk/Acad/IBLS/II/em/
Royal Microscopical Society: http://www.rms.org.uk
Scottish Microscopy Group: http://www.gla.ac.uk/ibls/II/em/SMG/smgnew.html


==============================Original Headers==============================
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From: microscopytoday-at-tampabay.rr.com
Date: Tue, 1 Nov 2005 11:36:28 -0600
Subject: [Microscopy] November 2005 Microscopy Today Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the November 2005 Microscopy Today table of contents. I will
close the subscription list for this issue on Monday, November 7, 2005.

Microscopists in North America and MSA members anywhere may have free
subscriptions. Anyone else may subscribe for US$35 per year (to
PARTIALLY cover postage). All subscriptions at
http://www.microscopy-today.com Thank you. Non-Qualified subscription
rates will increase to US$50 in 2006.

Ron Anderson, Editor
==================================

Very Cool Clathrin
Stephen W. Carmichael, Mayo Clinic

Metallography for the European Copper Age: Research on the Axe-Blade of
the Glacier-Mummy from the Ötztaler Alps in Tyrol
Gerhard O. Sperl, Institute for Historical Materials, Leoben, Austria

Microscopy and Microbes at Plum Island: Protecting America’s Livestock
Thomas G. Burrage, Plum Island Animal Disease Center, NY

Color Metallography
George F. Vander Voort, Buehler Ltd, Lake Bluff, Illinois

Characterization of Solids from Oilfield Emulsions
Richard W. Cloud,† Rebecca L. Ramsey,‡ Robert A. Pultz,‡ and Michael K.
Poindexter‡, † Nalco Company, Naperville, Illinois; ‡ Nalco Energy
Services, Sugar Land, Texas

Automated, Robotic Preparation of Vitrified Samples for 2D and 3D Cryo
Electron Microscopy
P. M. Frederik1 and M.H. Storms2; 1Univ. Maastricht, The Netherlands, 2.
FEI Company, Achtseweg Noord Eindhoven, The Netherlands

Ex-Situ “Auto Lift” Technique for TEM Sample Preparation
Garth “Brian” Cook, Micro Optics of Florida, Davie, Florida

Writing Nano-Scale Patterns on Insulators Using Variable Pressure
Electron-Beam Lithography
Floyd Miller and David Frey,* Lehigh University Bethlehem, PA and *Carl
Zeiss SMT Inc
.
Microscopy for Children
Caroline Schooley, MSA Project MICRO

Fostering LIMS Development Through Open Standards
Avrum Goodblatt, U. Penn School of Medicine, Philadelphia

50th Annivsary Celebrations of Atomic-Resolution Imaging
Thomas F. Kelly and Allan J. Melmed

Attaching Spheres to Cantilevers for Colloidal Probe Force Measurements:
A Simplified Technique
Yang Gan, University of Newcastle, Callaghan, NSW, Australia

Nissl: The Man, The Stain and The Substance
John A. Kiernan, The Univ. of Western Ontario London, Canada

Industry News
NetNotes
Index of Advertisers


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From: wgunning-at-meduohio.edu
Date: Wed, 2 Nov 2005 07:27:05 -0600
Subject: [Microscopy] viaWWW: MSA Award Nominations Reminder

Contents Retrieved from Microscopy Listserver Archives
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Email: wgunning-at-meduohio.edu
Name: WT Gunning

Organization: Medical University of Ohio

Title-Subject: [Filtered] MListserver: MSA Award Nominations Reminder

Question: Please note that MSA Awards nominations are due on December 15th, 2005.

2006 MICROSCOPY SOCIETY OF AMERICA AWARDS

The Distinguished Scientist Awards
(Physical & Biological Sciences)

The Burton Medal

The Outstanding Technologist Awards
(Physical & Biological Sciences)

The Mort Maser Distinguished Service Award

All MSA Members are encouraged to nominate candidates for these awards to recognize our eminent Scientific and Society leaders. Details available from the MSA Business Office.

Bostrom Corp.
230 East Ohio, Suite 400
Chicago, IL 60611
Toll-free: 1-800-538-3672
Tel: 312-644-1527
Fax: 312-644-8557
Email: BusinessOffice-at-microscopy.org

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From: camiller-at-anatomy.iupui.edu
Date: Wed, 2 Nov 2005 07:32:55 -0600
Subject: [Microscopy] viaWWW: Spring Experimental Biology 2006 Meeting

Contents Retrieved from Microscopy Listserver Archives
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Email: camiller-at-anatomy.iupui.edu
Name: Caroline Miller

Organization: Indiana University School of Medicine

Title-Subject: [Filtered] MListserver:

Question: We are approaching an abstract deadline for the Spring Experimental Biology 2006 Meeting in San Francisco April 1-5, 2006 (a national meeting with an attendance of about 15,000 people, see FASEBís website www.faseb.org). There is a platform session entitled ìElectron Microscopy as a 21st Century Toolî with a 15 minute talks. This platform session is entitled ìElectron Microscopy as a 21st Century Toolî and sponsored by the American Association of Anatomists-AAA and is being supported financially, in part, by the Indiana Microscopy Society and a request support from the MSA will be submitted. If you are interested in presenting some of your cutting edge, biological EM data, please consider submitting an abstract for the session. You will need to submit an abstract (the website URL for submission of an abstract is below). I will try to get sufficient funding so that all who present in this session can get reimbursed for the abstract fee as well as your registration and other associated expenses (hopefully) after the meeting. Make sure you submit for the AAA platform session entitled ìElectron Microscopy as a 21st Century Toolî.


Abstract submission deadline is November 7, 2005 . Thank you for considering submitting for this meeting



Abstract submission website (http://submissions.miracd.com/eb2006/login.asp)





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From: Willem.Wennekes-at-comcast.net
Date: Wed, 2 Nov 2005 07:44:18 -0600
Subject: [Microscopy] [Filtered] AskAMicroscopist: Aperture vs Current for EDX analysis

Contents Retrieved from Microscopy Listserver Archives
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This Question was submitted to Ask-A-Microscopist by (Willem.Wennekes-at-comcast.net)
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Email: Willem.Wennekes-at-comcast.net
Name: Willem Wennekes

Organization: UES

Education: Graduate College

Location: Dayton, OH, USA

Title: edx

Question: Hi, What would be the benefit of using a larger objective apperture instead of increasing the probe current in order to increase the beam current for edx analysis and x-ray mapping?

Cheers,
Willem

---------------------------------------------------------------------------

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From: David.R.Hull-at-nasa.gov
Date: Wed, 2 Nov 2005 10:21:26 -0600
Subject: [Microscopy] Re: [Filtered] AskAMicroscopist: Aperture vs Currentfor EDX analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

}
} Email: Willem.Wennekes-at-comcast.net
} Name: Willem Wennekes
}
} Organization: UES
}
} Education: Graduate College
}
} Location: Dayton, OH, USA
}
} Title: edx
}
} Question: Hi, What would be the benefit of using a larger
} objective apperture instead of increasing the probe current in order
} to increase the beam current for edx analysis and x-ray mapping?
}
} Cheers,
} Willem

The benefit is that you keep your small aperture clean for imaging.

==============================Original Headers==============================
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From: kenconverse-at-qualityimages.biz
Date: Wed, 2 Nov 2005 11:01:30 -0600
Subject: [Microscopy] [Filtered] AskAMicroscopist: Aperture vs Current for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Willem,
When you increase your probe size (demagnify the source less), the spray
electrons from each crossover tend to be captured closer to the beam path
thereby creating heavier contamination buildup nearer to the beam. This can
result in faster degradation of your imaging capabilities and the need for
more frequent cleaning of the column.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
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Sent: Wednesday, November 02, 2005 8:47 AM
To: kenconverse-at-qualityimages.biz

This Question was submitted to Ask-A-Microscopist by
(Willem.Wennekes-at-comcast.net)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
Tuesday, November 1, 2005 at 21:49:12
Remember to consider the Grade/Age of the student when considering the
Question
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Email: Willem.Wennekes-at-comcast.net
Name: Willem Wennekes

Organization: UES

Education: Graduate College

Location: Dayton, OH, USA

Title: edx

Question: Hi, What would be the benefit of using a larger objective
apperture instead of increasing the probe current in order to increase the
beam current for edx analysis and x-ray mapping?

Cheers,
Willem

---------------------------------------------------------------------------

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From: gary-at-gaugler.com
Date: Wed, 2 Nov 2005 11:34:55 -0600
Subject: [Microscopy] Re: AskAMicroscopist: Aperture vs Current for EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What are the options for increasing probe current? I.e., what
things can you do to increase it? yes, increasing the aperture
size will increase probe current. However, it increases probe
diameter. Another option is to increase KV. This will greatly
increase volumetric interacion such that more info comes from
deeper in the specimen. It is a constant trade-off problem.

Before changing anything above, be sure your specimen is at
the optimal analytical working distance (WD) for the EDS.
If you don't have that value, you can find it by experimentation.
Just start at a relatively long WD, check the counts per second
and then start reducing WD. Keep checking the cps at each
successive reduction in WD. The cps will start to increase and
then decrease. The WD at max cps is your analytical WD. Work
at this WD.

Now, back to aperture size and KV. I'd start with KV first.
Based on the highest Z you are looking at, you will need about
2X the eV of the line value you are collecting. Look at the M, K
and L series values and determine the necessary KV. E.g., Aluminum.
Z=13. No L-series lines. Ka=1.486KeV. Kb=1.553KeV. No N-series.
So, K-series are the only ones (BTW, Ka is the one used for calibration
along with Cu Ka at 8.040KeV.). So for Al, 5KV would probably
be a good value. If the specimen is bulk, then higher KV is OK
and would increase probe current and cps.

Take an extreme such as W (Z=74). Here, we have lines at all
shell series. However, not all of them are practical for normal
SEM. Ka=59.305KeV (can't use this since KV would need to be about
120KV). Same problem with Kb. Ma=1.774KeV. Sorting through the
other lines, La pops up at 8.394KeV. So 2X this would be 16KV.
So, 18KV-20KV would be appropriate. But at this KV, volumetric
interaction would be very high. High Z and high KV will result
in skyrocketing cps. So, smaller aperture would be necessary
to keep dead time down to {30% or so. If you want to quant,
this is probably the appropriate condition. However, for mapping,
if you do not have a volumetric interaction situation, and you
do not need high resolution, you can increase KV and aperture
size to increase cps. Keep in mind that most EDS systems need
cps to be such that dead time is {=35% or thereabouts. Increasing
cps with resulting higher DT simply throws away data since the
pulse processor cannot handle the high number of counts. Each
system has their own limit on this. Just look at DT to be sure
the processor is not overloaded.

Again, if the specimen is bulk versus very small, then increasing
aperture size will increase probe current and cps at the expense of
resolution. But for low mag, bulk specimens, not much of an issue.

Hope this helps.

gary g.







At 05:45 AM 11/2/2005, you wrote:

} November 1, 2005 at 21:49:12
} Remember to consider the Grade/Age of the student when considering
} the Question
} ---------------------------------------------------------------------------
} Please reply to both Willem.Wennekes-at-comcast.net as well as to
} the Microscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: Willem.Wennekes-at-comcast.net
} Name: Willem Wennekes
}
} Organization: UES
}
} Education: Graduate College
}
} Location: Dayton, OH, USA
}
} Title: edx
}
} Question: Hi, What would be the benefit of using a larger
} objective apperture instead of increasing the probe current in order
} to increase the beam current for edx analysis and x-ray mapping?
}
} Cheers,
} Willem
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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From: dhitrys-at-qimaging.com
Date: Wed, 2 Nov 2005 11:56:12 -0600
Subject: [Microscopy] Online Seminar: Selecting a Digital Camera for Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Announcing an interactive, web-based seminar:

===================================================
"Selecting a Digital Camera for Light Microscopy."
===================================================

Details are below.

Connection lines are limited, so reserve yours now. There is no charge to
participate in this on-line seminar.

For Wednesday, 9-November, 10:00 AM (New York time), pre-register at:
https://premconf.webex.com/premconf/j.php?ED=85888757&RG=1

For Thursday, 10-November, 11:00 AM (New York time, pre-register at:
https://premconf.webex.com/premconf/j.php?ED=85888782&RG=1

[ If any of the links have wrapped, please re-build it in your web browser's
address bar. The line begins with "https" and ends with "RG=1" ]


Details:
============

"Selecting a Digital Camera for Light Microscopy."
Presented by David Hitrys, QImaging Corporation

Attendee's will learn about digital cameras, how to interpret their
performance specifications, and will leave with enhanced skills for
critically analyzing a camera's suitability for various applications.

Outline of Topics:

-Understanding Digital Camera Parameters:
-Camera Sensitivity
-Noise Limitations
-Dynamic Range (Full-Well Capacity)
-Use of Gain
-Color Acquisition Options
-Note on Under- and Over-Sampling
-Matching Pixel Size to Optical Resolution
-Questions from the Audience

Presented by QImaging Corporation, makers of precision cameras for
microscopy, the information imparted will be broadly useful to anyone
striving to make the best camera choice for their imaging goals.

This seminar requires that attendees use a Java-enabled browser with a high
bandwidth connection. Audio is via toll-free telephone.

There is no charge to participate in this on-line seminar.

==============================Original Headers==============================
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From: beth-at-plantbio.uga.edu
Date: Wed, 2 Nov 2005 12:43:04 -0600
Subject: [Microscopy] support film - DuraSiN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
Has anyone used the new TEM support films from DuraSiN?
I'd appreciate hearing your opinions on this product.

SINcerely,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
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From: laable-at-solutia.com
Date: Wed, 2 Nov 2005 13:30:32 -0600
Subject: [Microscopy] SEM - repair service

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Is anyone aware of an independent company who offers yearly service contracts
on LEO field emission SEMS. I would appreciate any info possible.

Thanks,
Lori Ables
laable-at-solutia.com


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From: schamber-at-aspexllc.com
Date: Wed, 2 Nov 2005 13:54:05 -0600
Subject: [Microscopy] AskAMicroscopist: Aperture vs Current for EDX

Contents Retrieved from Microscopy Listserver Archives
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The statement that "increasing the aperture size ... increases probe
diameter" is not always true. The relationship between beam diameter
and beam current can be found in many references (e.g., equation 2.14 of
the 2nd edition of Goldstein). What this equation shows is that all
four of the terms that control beam diameter vary as the beam half-angle
(alpha) which is proportional to final aperture diameter. However, two
of the terms involve alpha in the numerator, and two in the denominator.
Thus, for any given probe current (Ip) there is an optimum aperture
size that gives the minimum beam diameter. Either a smaller or larger
aperture than called for by the minimum condition will make the beam
diameter larger.

Thus one very good reason for sometimes using a larger aperture size to
increase beam current rather than just cranking up the Spot control
(less demagnification) is to better match the condition for minimum spot
size .

Fred Schamber
ASPEX Corp.

gary-at-gaugler.com wrote:
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} What are the options for increasing probe current? I.e., what
} things can you do to increase it? yes, increasing the aperture
} size will increase probe current. However, it increases probe
} diameter. Another option is to increase KV. This will greatly
} increase volumetric interacion such that more info comes from
} deeper in the specimen. It is a constant trade-off problem.
}
} Before changing anything above, be sure your specimen is at
} the optimal analytical working distance (WD) for the EDS.
} If you don't have that value, you can find it by experimentation.
} Just start at a relatively long WD, check the counts per second
} and then start reducing WD. Keep checking the cps at each
} successive reduction in WD. The cps will start to increase and
} then decrease. The WD at max cps is your analytical WD. Work
} at this WD.
}
} Now, back to aperture size and KV. I'd start with KV first.
} Based on the highest Z you are looking at, you will need about
} 2X the eV of the line value you are collecting. Look at the M, K
} and L series values and determine the necessary KV. E.g., Aluminum.
} Z=13. No L-series lines. Ka=1.486KeV. Kb=1.553KeV. No N-series.
} So, K-series are the only ones (BTW, Ka is the one used for calibration
} along with Cu Ka at 8.040KeV.). So for Al, 5KV would probably
} be a good value. If the specimen is bulk, then higher KV is OK
} and would increase probe current and cps.
}
} Take an extreme such as W (Z=74). Here, we have lines at all
} shell series. However, not all of them are practical for normal
} SEM. Ka=59.305KeV (can't use this since KV would need to be about
} 120KV). Same problem with Kb. Ma=1.774KeV. Sorting through the
} other lines, La pops up at 8.394KeV. So 2X this would be 16KV.
} So, 18KV-20KV would be appropriate. But at this KV, volumetric
} interaction would be very high. High Z and high KV will result
} in skyrocketing cps. So, smaller aperture would be necessary
} to keep dead time down to {30% or so. If you want to quant,
} this is probably the appropriate condition. However, for mapping,
} if you do not have a volumetric interaction situation, and you
} do not need high resolution, you can increase KV and aperture
} size to increase cps. Keep in mind that most EDS systems need
} cps to be such that dead time is {=35% or thereabouts. Increasing
} cps with resulting higher DT simply throws away data since the
} pulse processor cannot handle the high number of counts. Each
} system has their own limit on this. Just look at DT to be sure
} the processor is not overloaded.
}
} Again, if the specimen is bulk versus very small, then increasing
} aperture size will increase probe current and cps at the expense of
} resolution. But for low mag, bulk specimens, not much of an issue.
}
} Hope this helps.
}
} gary g.
}
}
}
}
}
}
}
} At 05:45 AM 11/2/2005, you wrote:
}
}
} } November 1, 2005 at 21:49:12
} } Remember to consider the Grade/Age of the student when considering
} } the Question
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} } Email: Willem.Wennekes-at-comcast.net
} } Name: Willem Wennekes
} }
} } Organization: UES
} }
} } Education: Graduate College
} }
} } Location: Dayton, OH, USA
} }
} } Title: edx
} }
} } Question: Hi, What would be the benefit of using a larger
} } objective apperture instead of increasing the probe current in order
} } to increase the beam current for edx analysis and x-ray mapping?
} }
} } Cheers,
} } Willem
} }
} } ---------------------------------------------------------------------------
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From: CGoldsmith-at-cdc.gov
Date: Wed, 2 Nov 2005 17:53:05 -0600
Subject: [Microscopy] viaWWW: Used FEI EM410-LS is available

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This Question was submitted to the Microscopy Listserver
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Email: CGoldsmith-at-cdc.gov
Name: Cynthia Goldsmith

Organization: Centers for Disease Control and Prevention (CDC)

Title-Subject: [Filtered] MListserver:Used FEI EM410-LS is available

Question: The Centers for Disease Control and Prevention (CDC) has a used FEI EM410-LS that is available for transfer to a government facility (federal, state, or local) or an educational institution (university, college, high school, etc.). Microscope is 21 years old, and in good condition. Please contact me off-line at CGoldsmith-at-cdc.gov for more information.


Cynthia S. Goldsmith, M.S.
Infectious Disease Pathology Activity
Centers for Disease Control and Prevention (CDC)
Phone: (404)639-3306
Fax: (404)639-1377



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From: gary-at-gaugler.com
Date: Wed, 2 Nov 2005 18:16:54 -0600
Subject: [Microscopy] Re: AskAMicroscopist: Aperture vs Current for EDX

Contents Retrieved from Microscopy Listserver Archives
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This follows published info. However, the question was
about aperture size. The other variable of condenser
squeeze or relaxation is something else to throw into the
pot. We don't know if his system has a condenser control.
Probably so.

He was looking for increase in probe current. I figure
that it is safe to say that everything else being equal,
increasing the aperture size will increase probe current.
That is really all he wants to do/know--notwithstanding all
the other nuances. He just wants to know about a larger
final aperture size.

gary g.



At 11:55 AM 11/2/2005, you wrote:



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From: beth-at-plantbio.uga.edu
Date: Thu, 3 Nov 2005 09:31:16 -0600
Subject: [Microscopy] silicon nitride support film grids

Contents Retrieved from Microscopy Listserver Archives
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Hi again,
I've been asked to rephrase my question about silicon nitride membrane
window grids.
If you use either the SPI silicon nitride membrane window grids or the
EMS DuraSiN silicon nitride membrane window grids I'd like to hear your
opinion of them.
Thanks,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



==============================Original Headers==============================
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From: schamber-at-aspexllc.com
Date: Thu, 3 Nov 2005 11:16:45 -0600
Subject: [Microscopy] AskAMicroscopist: Aperture vs Current for EDX

Contents Retrieved from Microscopy Listserver Archives
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I think there are two issues here. One is the "physics" question (what
does theory say? ) and the other a practical question (what happens in
an actual instrument operating in a normal way?).

First the physics. Here the ultimate limit is diffraction. Regardless
of the source type, a too-small aperture will cause a diffraction
spreading of the beam. The simplest way to understand this is as a
manifestation of the Heisenberg uncertainty principle -- when the
lateral location of the electron is too tightly constrained by the
aperture, then the lateral uncertainty in the momentum increases and
there is broadening. So if one were operating a SEM in a
difraction-limited mode, increasing the aperture size would be the most
sensible way to increase the beam current.

Most thermionic (not FE) SEMs are not operated anywhere near the
diffraction limit. Usually the dominant limit to resolution is
spherical aberration and that term varies as aperture size to the third
power. The simple quadrature approximation shown as equation 2.14 in
Goldstein (2nd Ed) is generally regarded as valid for thermionic SEMs,
but is not valid for any instrument operating near the diffraction
limit. Here one must use much more complex wave-function calculations
-- it's been a long time since I did this, but the role of the aperture
is the same. There is an optimum aperture size for any given beam
current and either a smaller or larger aperture than that optimum will
increase the beam diameter.

Now for the practical. What actually happens when you turn a knob or
change an aperture on a particular instrument depends on the details of
how that instrument was designed and is being operated. When a SEM
employs a "virtual" aperture (the physical aperture doesn't reside in
the principal plane of the probe-forming lens) then it is possible to
change the *effective* aperture size by manipulating the condenser
lens(es) and thus the effective aperture size can be optimized by the
control software to be optimal for that probe current without changing
the physical aperture. Even if that is not the case, it's usually
impractical or inconvenient to optimally match the physical aperture to
its ideal size (apertures tend to be available in rather coarse diameter
steps). Consequently, for modest changes in beam current, just turning
the "Spot" control (or whatever you call the control that regulates the
condenser current) is the simple practical expedient. But in more
extreme cases, where one wants to drastically increase the beam current
but doesn't want to unduly enlarge the spot, one is well advised to pay
attention to the physics -- the principle works.

Now, back to the original question that was asked. "What would be the
benefit of using a larger objective apperture instead of increasing the
probe current in order to increase the beam current for edx analysis and
x-ray mapping?" There are certainly many situations where choosing a
larger aperture size will permit the higher probe current required for
x-ray analysis to be achieved with minimal degradation of beam diameter.
That is a practical reality in many situations. There may be other
good reasons, and this answer won't apply to all situations. But it is
a mechanism that needs to be considered given the limited information
provided. So far from being a "nuance," depending on the instrument
being referenced, this may in fact be the most pertinent answer to the
question posed.

Fred Schamber
ASPEX Corp.

gary-at-gaugler.com wrote:
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}
} This follows published info. However, the question was
} about aperture size. The other variable of condenser
} squeeze or relaxation is something else to throw into the
} pot. We don't know if his system has a condenser control.
} Probably so.
}
} He was looking for increase in probe current. I figure
} that it is safe to say that everything else being equal,
} increasing the aperture size will increase probe current.
} That is really all he wants to do/know--notwithstanding all
} the other nuances. He just wants to know about a larger
} final aperture size.
}
} gary g.
}
}
}
} At 11:55 AM 11/2/2005, you wrote:
}
}
}
}
} } ----------------------------------------------------------------------------
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From: kenconverse-at-qualityimages.biz
Date: Thu, 3 Nov 2005 14:18:35 -0600
Subject: [Microscopy] SEM - repair service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lori,
I've had a number of requests in the past year or so to service LEO
equipment. The problem is that they don't provide schematics and a third
party can't come in and have any hope of being competent without them.

As far as I know, every other manufacturer provides schematics with their
system.

Perhaps someone from LEO would like to comment.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: laable-at-solutia.com [mailto:laable-at-solutia.com]
Sent: Wednesday, November 02, 2005 2:34 PM
To: kenconverse-at-qualityimages.biz

Is anyone aware of an independent company who offers yearly service
contracts
on LEO field emission SEMS. I would appreciate any info possible.

Thanks,
Lori Ables
laable-at-solutia.com


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From: ramadanhany-at-gmail.com
Date: Thu, 3 Nov 2005 20:10:34 -0600
Subject: [Microscopy] A favor needed....Thin film technology handbook....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi guys,

I am writing to see if there is anyone has the following book:
******
Thin film technology handbook
By: Aicha A. R. Elshabini-Riad, Fred D. Barlow III.
c1998
******

It is not available now at the university library and all what I need
is to read pages 27:34, so I hope if there is someone has it and scan
those pages and send them to me. I really need them urgently.


Thanks in advance and I appreciate your time and help.

Best Regards

Hany

--
**********************************************************
Hany Ramadan
Graduate student
Chemistry department
McMaster university, Hamilton, Ontario, Canada
905-525-9140 x: 26322
elsayeh-at-mcmaster.ca
**********************************************************


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9, 23 -- Subject: A favor needed....Thin film technology handbook....
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From: akoorts-at-medic.up.ac.za
Date: Fri, 4 Nov 2005 07:37:28 -0600
Subject: [Microscopy] [Filtered] AskAMicroscopist: Grid support films

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This Question was submitted to Ask-A-Microscopist by (akoorts-at-medic.up.ac.za)
from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, November 4, 2005 at 01:39:39
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Email: akoorts-at-medic.up.ac.za
Name: Alida Koorts

Organization: University of Pretoria

Education: Graduate College

Location: South Africa

Title: Grid support films

Question: I loose a lot of sections from uncoated 200 mesh nickel grids during immunolocalization (including autoclave antigen retrieval) and in situ hybridization (including autoclave pre-treatment). Suggestions to appropriate support films that would not interfere with the immunolocalization and in situ hybridization reactions?

Regards

Alida

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From: marta.taules-at-uab.es
Date: Fri, 4 Nov 2005 07:38:46 -0600
Subject: [Microscopy] viaWWW: purity of ethane for cryo fixation?

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Email: marta.taules-at-uab.es
Name: Marta Taules

Organization: UAB

Title-Subject: [Filtered] MListserver:

Question: Dear collegues,

I would like to know which should be the purity of ethane in order to apply the immersion cryo fixation with forceps injector (CPC equipment from Leica).

Thank you in advanced

---------------------------------------------------------------------------

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From: ramadanhany-at-gmail.com
Date: Fri, 4 Nov 2005 12:28:42 -0600
Subject: [Microscopy] Re: A favor needed....Thin film technology handbook....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks Jim for your email, here are the details:
This book is available at the library but it is checked out and it may
take more than 10 days to recall it and I need it for a report that is
due in a few days. I can not use ILL to get it because the library
already has it but it is just checked out.

Thanks

Best Regards

Hany


On 11/4/05, Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} wrote:
} Hany
}
} Your library has a ILL (Inter Library Loan) department
} that will do that for you.
}
} regards,
}
} Jim
}
} } From mail-at-ns.microscopy.com Thu Nov 3 21:14:19 2005
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} } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
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} } ----------------------------------------------------------------------------
} }
} } Hi guys,
} }
} } I am writing to see if there is anyone has the following book:
} } ******
} } Thin film technology handbook
} } By: Aicha A. R. Elshabini-Riad, Fred D. Barlow III.
} } c1998
} } ******
} }
} } It is not available now at the university library and all what I need
} } is to read pages 27:34, so I hope if there is someone has it and scan
} } those pages and send them to me. I really need them urgently.
} }
} }
} } Thanks in advance and I appreciate your time and help.
} }
} } Best Regards
} }
} } Hany
} }
} } --
} } **********************************************************
} } Hany Ramadan
} } Graduate student
} } Chemistry department
} } McMaster university, Hamilton, Ontario, Canada
} } 905-525-9140 x: 26322
} } elsayeh-at-mcmaster.ca
} } **********************************************************
} }
} }
} } ==============================Original Headers==============================
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} } 9, 23 -- From: Hany Ramadan {ramadanhany-at-gmail.com}
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} } 9, 23 -- Subject: A favor needed....Thin film technology handbook....
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}


--
**********************************************************
Hany Ramadan
Graduate student
Chemistry department
McMaster university, Hamilton, Ontario, Canada
905-525-9140 x: 26322
elsayeh-at-mcmaster.ca
**********************************************************


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From: guderjan-at-yahoo.com
Date: Fri, 4 Nov 2005 15:51:47 -0600
Subject: [Microscopy] Zeiss Model 109

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Maya Research Program is a non-profit corporation that
supports archaeological research and is affiliated
with Texas Christin University. We have been given a
Zeiss Model 109 SEM. It was taken out of service by
the University of Houston, but apparently is in very
good condition.

We wish to sell this instrument. Does anyone know of
someone who deals in such equipment?

Many thanks in advance.

Tom Guderjan





__________________________________
Yahoo! Mail - PC Magazine Editors' Choice 2005
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From: ac.richardson2-at-btinternet.com
Date: Sat, 5 Nov 2005 12:32:35 -0600
Subject: [Microscopy] TEM: freeze substitution

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Hello all,

A quick technical question - we are trying to do freeze substitution using
2%Osmium in acetone, but it goes black as we warm from -90 to -60. Have
you done this and if so is this normal or is there something wrong with
our Os/acetone? Acetone has been kept over a molecular sieve in dialysis
tubing,
I have read somewhere that molecular sieves should be avoided, could
this be the problem?
Acetone is at -20c before adding to osmium and then immediately frozen.





A.C.Richardson
Experimental Officer
School of Biological and Biomedical Science
Centre for Molecular Imaging
University of Durham
Science site
South Rd
Durham
England
E-mail: a.c.richardson-at-durham.ac.uk

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From: dsherman-at-purdue.edu
Date: Sat, 5 Nov 2005 15:54:57 -0600
Subject: [Microscopy] Fixing insect eyes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have a project coming up for preparation of mosquito eyes for both LM and
TEM examination. I was planning on using a glut-PAF fix in cacodylate
buffer followed by osmium tetroxide, ETOH dehydration and embedding in epoxy
generic resin.

Options are incubation with tannic acid and en bloc staining with UA prior
to dehydration.

I would appreciate some suggestions as to preparation protocols. Have any
of you tried the tannic acid incubation? If so, at what concentration and
when...in both glut/PAF and Os or alone after glut/PAF? Have you used it at
RT or 4oC? Has en bloc UA been helpful in increasing contrast in eye tissue?

Any suggestions would be most welcome.

Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www3.agriculture.purdue.edu/microscopy


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From: dswilliams-at-ucsd.edu
Date: Sun, 6 Nov 2005 12:34:18 -0600
Subject: [Microscopy] viaWWW: POSTDOCTORAL POSITION in cell biology @ UCSD

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Email: dswilliams-at-ucsd.edu
Name: DS Williams

Organization: UCSD

Title-Subject: [Filtered] POSTDOCTORAL POSITION in cell biology -at- UCSD

Question: POSTDOCTORAL POSITION in cell biology
UCSD SCHOOL OF MEDICINE
LA JOLLA, CALIFORNIA

Experience in microscopy required, preferably electron microscopy. Studies will be on cellular mechanisms in the retina and potential therapies for retinal degeneration. They will also include collaborative studies on other neurodegenerative disorders. See lab web site for further information about the lab: http://medicine.ucsd.edu/williams/. There will be ample opportunity to develop state-of-the-art skills and collaborations in the rich scientific environment of La Jolla. Start date: negotiable. Salary: NIH scale. Contact: Dr David Williams, Departments of Pharmacology and Neurosciences, UCSD School of Medicine, La Jolla, CA 92093-0912. Email: {DSWILLIAMS-at-UCSD.EDU}


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From: richard.beanland-at-bookham.com
Date: Mon, 7 Nov 2005 05:56:40 -0600
Subject: [Microscopy] RE: Reflecting objectives

Contents Retrieved from Microscopy Listserver Archives
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Hi Folks,
while rooting around in a cupboard for an eyepiece with graticule this morning I came across a couple of Beck "reflecting objectives" in their little mahogany boxes. I would dearly like to know what they are used for - it's clear that they focus parallel light to a point... Do they have a use in microscopy, or have I just found a component from a rather old experiment on an optical bench?

Many thanks

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________

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From: frank.karl-at-degussa.com
Date: Mon, 7 Nov 2005 06:44:54 -0600
Subject: [Microscopy] Reflecting objectives

Contents Retrieved from Microscopy Listserver Archives
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Reflective objectives had several different proposes. One was an attempt
to create better images as compared to the crude glass lens of the late
1800 early 1900's. They also had a purpose as they would gather and focus
IR and UV. Of course you needed to use film to collect and "see" the
image. I seem to remember that later ones sometimes contained a glass lens
to assist in correcting for visible light images. Of course you lost all
the UV /I R that the glass filtered.

Modern IR collecting objectives used in micro spectroscopy are reflective
objectives revisited and we come full circle....

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


This e-mail transmission, and any documents, files or previous e-mail
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richard.beanland-at-
bookham.com To: frank.karl-at-degussa.com
cc:
11/07/2005 06:58 Subject: [Microscopy] RE: Reflecting objectives
AM
Please respond to
richard.beanland








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Hi Folks,
while rooting around in a cupboard for an eyepiece with
graticule this morning I came across a couple of Beck "reflecting
objectives" in their little mahogany boxes. I would dearly like to know
what they are used for - it's clear that they focus parallel light to a
point... Do they have a use in microscopy, or have I just found a component
from a rather old experiment on an optical bench?

Many thanks

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________

=======================================================================
This e-mail is intended for the person it is addressed to only. The
information contained in it may be confidential and/or protected by
law. If you are not the intended recipient of this message, you must
not make any use of this information, or copy or show it to any
person. Please contact us immediately to tell us that you have
received this e-mail, and return the original to us. Any use,
forwarding, printing or copying of this message is strictly prohibited.
No part of this message can be considered a request for goods or
services.
=======================================================================


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From: stranen_connelly-at-yahoo.com
Date: Mon, 7 Nov 2005 08:00:11 -0600
Subject: [Microscopy] Re: Fixing insect eyes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debbie,

I have done work with Drosophila eyes and the
following points may be of interest to you:

There are bad results if one fixes the whole head for
not enough fix gets through the "neck".

UA does really increase the contrast. My way was 1%UA
in the refrigerator over night (cold and dark) since
specimens were usually ready to be fixed in the
afternoon and I had used a combination of glut. and
OsO4 on ice for fixation, but an hour or so also
works.

If looking at nerves or cytoskeleton a (good quality)
Acetone dehydration works better than EtOH.

The tannic acid might give some interesting results
but it was not tried in my experiments. If I recall
correctly when using tannic acid it was with the glut.
at room temp. and freshly made but others will no
doubt be better on this point.

Standard epoxy embedding is fine.

I had wanted to try the microwave techniques on the
insect heads to see if the head dissection could be
avoided - everything in its proper place - but did not
have access to a machine until after the head
experiments were over.

Lots of luck!
Pat Connelly
stranen_connelly-at-yahoo.com
(currently seeking employment)

--- dsherman-at-purdue.edu wrote:

} We have a project coming up for preparation of
} mosquito eyes for both LM and
} TEM examination. I was planning on using a glut-PAF
} fix in cacodylate
} buffer followed by osmium tetroxide, ETOH
} dehydration and embedding in epoxy
} generic resin.
}
} Options are incubation with tannic acid and en bloc
} staining with UA prior
} to dehydration.

} I would appreciate some suggestions as to
} preparation protocols. Have any
} of you tried the tannic acid incubation? If so, at
} what concentration and
} when...in both glut/PAF and Os or alone after
} glut/PAF? Have you used it at
} RT or 4oC? Has en bloc UA been helpful in increasing
} contrast in eye tissue?

} Debby Sherman, Manager Phone:
} 765-494-6666
} Life Science Microscopy Facility FAX:
} 765-494-5896
} Purdue University E-mail:
} dsherman-at-purdue.edu
} S-052 Whistler Building
} 170 S. University Street
} West Lafayette, IN 47907
} http://www3.agriculture.purdue.edu/microscopy





__________________________________
Yahoo! Mail - PC Magazine Editors' Choice 2005
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From: bharris-at-uoguelph.ca
Date: Mon, 7 Nov 2005 10:06:19 -0600
Subject: [Microscopy] networking EM computers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good Morning: We have 2 TEMs with a 3rd coming that are acquiring digital
images. We would like to store these images centrally and deliver them to
customers over the internet. To this end we have purchased the SIS Web Racer
program but don't know how to continue. Our IT dept won't support us. If anyone
out there is doing this I would much appreciate a note on how to construct such
a network from the ground up. Thanks bob

Guelph Regional Imaging Facility
Dept.of Molecular and Cellular
Biology
New Science Complex
488 Gordon St.
Univ of Guelph
Guelph,On, Canada
N1G 2W1
ph: 519-824-4120 ext. 56409/58962
Fax: 519-837-1802

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From: john.mardinly-at-intel.com
Date: Mon, 7 Nov 2005 11:09:15 -0600
Subject: [Microscopy] Reflecting objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Bob,

Have at look at the Open Microscopy Environment - Jason Smedlow of OME gave
a talk at UCL recently about doing such a thing using "a single relational
database with XML for data migration". See the www.openmicroscopy.org . I
don't know if they can provide one-to-one help but they would seem a good
starting point as they have such a system up and running (and I think
received a fair bit of funding to set it up).

Regards

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


----- Original Message -----
X-from: {bharris-at-uoguelph.ca}
To: {keith.morris-at-ucl.ac.uk}
Sent: Monday, November 07, 2005 4:14 PM


Another useful aspect of reflective objectives is that they can maintain
a large NA at a long working distance and hence get better resolution
than glass lenses at similar long working distance. Kind of like a
telescope......

John Mardinly
Intel

The copinions of the writer are not necessarily the opinions of Intel
Corporation.

-----Original Message-----
X-from: frank.karl-at-degussa.com [mailto:frank.karl-at-degussa.com]
Sent: Monday, November 07, 2005 4:45 AM
To: Mardinly, John

Reflective objectives had several different proposes. One was an
attempt
to create better images as compared to the crude glass lens of the late
1800 early 1900's. They also had a purpose as they would gather and
focus
IR and UV. Of course you needed to use film to collect and "see" the
image. I seem to remember that later ones sometimes contained a glass
lens
to assist in correcting for visible light images. Of course you lost
all
the UV /I R that the glass filtered.

Modern IR collecting objectives used in micro spectroscopy are
reflective
objectives revisited and we come full circle....

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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richard.beanland-at-

bookham.com To:
frank.karl-at-degussa.com

cc:

11/07/2005 06:58 Subject: [Microscopy]
RE: Reflecting objectives
AM

Please respond to

richard.beanland










------------------------------------------------------------------------
----

The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America


Hi Folks,
while rooting around in a cupboard for an eyepiece with
graticule this morning I came across a couple of Beck "reflecting
objectives" in their little mahogany boxes. I would dearly like to know
what they are used for - it's clear that they focus parallel light to a
point... Do they have a use in microscopy, or have I just found a
component
from a rather old experiment on an optical bench?

Many thanks

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________

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From: gary-at-gaugler.com
Date: Mon, 7 Nov 2005 12:07:21 -0600
Subject: [Microscopy] Re: networking EM computers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If your IT department won't help you, what do they actually
do? What purpose do they serve? That aside, you will need
some info from them. Do you already have a CAT-5 10/100BaseT
Ethernet connection to the Internet? If not, you will need one.
Then you will need to know if a computer connected to the cable
needs a static IP or if the main server does DHCP. Then you
need to know the DNS servers (primary and secondary) that your
place uses (you need their IP addresses).

Then, get a simple router like Linksys BEFSR41. This is a four
port switch router. Connect Internet cable to the router and
the other computers to the switch ports. I assume that each
PC has a NIC or built-in Ethernet. Use browser to connect to
router, configure it according to info and set up a private
Intranet either via DHCP or static addresses. Static is usually
preferred since you can define machine names directly in
c:\Windows\system32\drivers\etc\hosts, that way, everyone will
get the same copy of hosts and know what is what.

One machine will always have to be on for this to work. Whichever
this one is, use one of its disks as the distribution disk or
connect a USB/Firewire external disk or a removable disk bay in
the PC box. This will be your server PC.

All of the PCs can talk to each other and send and fetch data
from the "server" PC and send to Internet destinations.

Just an idea/suggestion. But this works.

gary g.




At 08:09 AM 11/7/2005, you wrote:


} Good Morning: We have 2 TEMs with a 3rd coming that are acquiring digital
} images. We would like to store these images centrally and deliver them to
} customers over the internet. To this end we have purchased the SIS Web Racer
} program but don't know how to continue. Our IT dept won't support
} us. If anyone
} out there is doing this I would much appreciate a note on how to
} construct such
} a network from the ground up. Thanks bob
}
} Guelph Regional Imaging Facility
} Dept.of Molecular and Cellular
} Biology
} New Science Complex
} 488 Gordon St.
} Univ of Guelph
} Guelph,On, Canada
} N1G 2W1
} ph: 519-824-4120 ext. 56409/58962
} Fax: 519-837-1802
}
} ==============================Original Headers==============================
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From: gcc-at-couger.com
Date: Mon, 7 Nov 2005 14:03:23 -0600
Subject: [Microscopy] Reflecting objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



richard.beanland-at-bookham.com wrote:

}
} Hi Folks,
} while rooting around in a cupboard for an eyepiece with graticule
;this morning I came across a couple of Beck "reflecting objectives" in
;their little mahogany boxes. I would dearly like to know what they
;are used for - it's clear that they focus parallel light to a point...

;Do they have a use in microscopy, or have I just found a component
;from a rather old experiment on an optical bench?

Hi Richard

The are almost certainly microscope objectives. Put one on a scope and see
how it works. The principle behind them is mirrors have almost the same
focal the same focal length over a very wide range of frequencies. The
small exception is explained on this Canon page on Near Field Microscopy
http://www.canon.com/technology/s_labo/light/004/01.html In the Dripping
Light selection it shows the a reflected wave is leaves the surface 1 wave
length away from where it strikes the surface. But for most practical uses
that does not effect us.

If they are low power and low NA the best way to use them for near infra
red and the UV that silicone sensors will capture is to see if they will
work as a prime lens for a video camera with no lenses to have different
focal points at 1000 nm light and 300nm light. The lens will cover a much
wider range of light than silicone will detect but you start taking about
a great deal of money for cameras. You also need a monochromator or filers
to make much sense of the images.

If you need higher resolution than a video camera will record. Three are
some modifiable 3 mega pixel camera such as the Aiptek 5100 that has
smile lens that is easy to replace that will 11 frames per second or 640 x
480 Video and a true 3 Mega pixel image on a memory card for $100. I have
on my desk I am converting. I am not sure if the IR/UV filter is in the
lens or on the sensor. But most of the fixed lens 3 mega pixel cameras can
be modified to take other lenses and the IR/UV filters removed.

If the Breck objectives are below 40x and you are ever going to put up for
surplus I want to be first on the list to be notified when you get rid of
them.

Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org


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From: sghoshro-at-NMSU.Edu
Date: Mon, 7 Nov 2005 14:18:12 -0600
Subject: [Microscopy] Hepatitis C infected tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everyone,

A graduate student is planning to fix hepatitis C infected human liver
tissue for TEM (fixation and embedding) work. I would like to know what
would be the best way to fix the fresh tissue and transport it from a
hospital. I am also interested to find out what precautionary measure we
should take since the student will be handling infected liver tissue. We
are planning to fix in glutaraldehyde in cacodylate/phosphate buffer. Is
the virus considered infectious after fixation? Any suggestion will be
greatly appreciated.

Thanks in advance.

Soumitra




*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/

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From: lkrupp-at-us.ibm.com
Date: Mon, 7 Nov 2005 16:05:44 -0600
Subject: [Microscopy] Where to buy epo-tek epoxy

Contents Retrieved from Microscopy Listserver Archives
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Hello-

I need to purchase Epo-tek 350ND epoxy, and am having trouble finding it
online. Does anyone know someone that carries it, or another contact
person?

Vendor replies are fine.

Thank you,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099


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From: lkrupp-at-us.ibm.com
Date: Mon, 7 Nov 2005 16:48:21 -0600
Subject: [Microscopy] Fw: Where to buy epo-tek epoxy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099
(408) 927-3856
----- Forwarded by Leslie E Krupp/Almaden/IBM on 11/07/2005 02:46 PM -----
|---------+----------------------------}
| | Dmrelion-at-aol.com |
| | |
| | 11/07/2005 02:45 |
| | PM |
|---------+----------------------------}
} -------------------------------------------------------------------------------------------------------------------------------|
| |
| To: Leslie E Krupp/Almaden/IBM-at-IBMUS |
| cc: |
| Subject: Re: [Microscopy] Where to buy epo-tek epoxy |
} -------------------------------------------------------------------------------------------------------------------------------|




Leslie,

Try going to

http://www.epotek.com/categories.asp?ID=3


The list a 353ND but their sales dept. should be able to help.

We buy their Epotek301 for use in mounting geological thin sections for
cathodoluminescence studies. Good optical and thermal properties and stands
up to the heat of the electron beam.

Don Marshall

Donald J. Marshall (Dr.)
RELION Industries
PO Box 12
Bedford, MA 01730
USA

781-275-4695 (phone)
781-271-0252 (FAX)

dmrelion-at-aol.com

http://www.excitingelectrons.com

"A weed is a flower out of place

In a message dated 11/7/2005 5:08:51 PM Eastern Standard Time,
lkrupp-at-us.ibm.com writes:

Hello-

I need to purchase Epo-tek 350ND epoxy, and am having trouble finding it
online. Does anyone know someone that carries it, or another contact
person?

Vendor replies are fine.

Thank you,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099

."


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From: gsosinsky-at-ucsd.edu
Date: Tue, 8 Nov 2005 08:09:47 -0600
Subject: [Microscopy] viaWWW: Postdoctoral/Staff position at the National Center for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
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Email: gsosinsky-at-ucsd.edu
Name: Gina Sosinsky

Organization: University of California, San Diego

Title-Subject: [Filtered] MListserver: Position available

Question: Postdoctoral/Staff position at the National Center for Microscopy and Imaging Research at the University of California, San Diego, CA

A postdoctoral or staff position in cryo-EM and 3D reconstruction to study connexin26 wild type and mutant structures is available immediately.
Our laboratory has isolated connexin26 gap junctions as in situ ordered two-dimensional crystals and as isolated hemichannels for analysis by electron microscopic structure determination, atomic force microscope imaging and biochemical studies. We are investigating the structure of wild type connexin26 connexons, functional mutants and treatments that result in changes in the pore size or shape to study structural mechanisms of connexon gating.

The successful candidate will have been awarded a Ph.D. or submitted (with oral examination pending), have experience in electron cryo-microscopy and image processing or three-dimensional reconstruction techniques. Experience in membrane purification techniques is preferred, but not essential. Excellent written and verbal skills are expected. The candidate will be working in a large interdisciplinary research center located in the University of California San Diego main campus and will have access to state of the art electron microscopes. For more information on our laboratory, please visit http://www.ncmir.ucsd.edu.
Please forward this message to all appropriate personnel. Applicants should send curriculum vitae, bibliography, a brief description of present research activities and plans and the names and contact information of three references to:

Dr. Gina Sosinsky

University of California at San Diego
1070 Basic Science Building MC 0608
9500 Gilman Drive
La Jolla, CA 92093-0608
858-534-0128 (office phone & voice mail)
858-534-4583 (lab phone)
858-534-7497 (fax)
gsosinsky-at-ucsd.edu (email)


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From: m_jarnik-at-fccc.edu
Date: Tue, 8 Nov 2005 08:10:51 -0600
Subject: [Microscopy] viaWWW: Embedding melanocytes

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Email: m_jarnik-at-fccc.edu
Name: Michal Jarnik

Organization: FCCC

Title-Subject: [Filtered] Embedding melanocytes

Question: We have some problems embedding melanocytes in Epon. Although the overall ultrastructure of the cells is typically good, the melanosomes lack the outer membrane. We tried several varieties of our standard embedding protocol (glutaraldehyde fixation followed by osmication, dehydration in EtOH and propylenoxide and embedding), but no real difference. Any suggestions would be welcome. Thanks.

---------------------------------------------------------------------------

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From: TindallR-at-missouri.edu
Date: Tue, 8 Nov 2005 10:13:10 -0600
Subject: [Microscopy] TEM: Intractable Pepper Redux

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Soumitra,

Your question was outside my experience, so I contacted a
virologist/microscopist I know, and she kindly sent me the following.

The required precautions sound quite serious and she suggested you
contact her with any questions.

Mike
------------
Any clinical specimen should be handled with "Universal Precautions".

There is a set of guidelines known to anyone who routinely collects
potentially
infectious material. I would let this person place the tissue into
fixative. These procedures entail the use of gloves, lab coat, face
protection, special procedures for not cutting or sticking oneself with
sharp objects, and use of a biosafety level 2 cabinet.

Whoever obtains the specimen from the patient (the surgeon? a pathology
technologist?) can place it directly into glutaraldehyde for transport.
This
will render the specimen non-infectious since the agent is an enveloped
virus
(flavivirus). (Prion material is not killed by aldehyde fixation, but all
human viruses are.) Ask the person obtaining the sample to cut it into
small
pieces or slivers if possible (a few mm). If you get a large chunk (e.g., a
cubic cm), let it sit in glutaraldehyde overnight to ensure that the
fixative
gets inside. Then take your 1 cubic mm slices for EM from the surface
that is
well fixed. The center may or may not be well fixed, depending on how
long the tissue sat before being placed into the glut. At this stage,
you can handle the
tissue just like any other non-infectious specimen.

Note that few good micrographs of HCV have been published, particularly from
infected human liver. Also, note that flaviviruses are small (40-60 nm)
enveloped RNA viruses, and that other things in the cell can resemble these
particles. Most diagnoses of HCV are made histologically on the
appearance of
the liver, by immunostaining, and/or serologically, but not by EM. Good
luck.


Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265


----- Original Message -----
X-from: sghoshro-at-NMSU.Edu

Dear Listers,

Back in the mists of history, about two or three years ago, I put a plea
for help on the listserver concerning pepper-like section contamination.
This stuff resisted every attempt to get rid of it---different
fixatives, four different buffer systems, various resins, five
different water supplies, stains (or no stains!), fixation with and
without osmium, different chemical suppliers, rewashing every piece of
glassware in the lab, prayer, curses, voodoo. Even a Kitchen Witch. We
tried all of the many suggestions from the dedicated denizens of the
list (which normally cure any problem we have), all to no avail. It
showed up in some tissues all of the time (especially retina), some
tissues some of the time, some tissues never, and was often localized on
membranes. We finally determined that it was osmium in part (although
we got some pepper even in unosmicated samples). So I discussed the
problem with a chemist who specialized in osmium chemistry. He mumbled
something about changes in the air in the lab and wished us luck as he
beat a hasty retreat. Must have been the haunted look on my face.

Finally, after almost two years, in desperation our lab director
suggested adding 0.01M 2-mercaptoethanol to the buffer washes after the
primary fixation step and in the osmium step. No more pepper. I
promised if we ever figured this one out, I would share it with the
list, so here you go.

The problem is that we never really figured it out, except for how to
make it go away. That's great on one hand, but fundamentally
frustrating on the other, since we never got a handle on why the
contamination appeared suddenly in the first place and took up residence
in the lab.

So, if you too have Intractable Pepper Syndrome, try the 2-ME. If you
have any thoughts on why this works, we (and Tom Phillips) would love to
hear them.

Relieved, but puzzled,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: phillipst-at-missouri.edu
Date: Tue, 8 Nov 2005 10:22:30 -0600
Subject: [Microscopy] Pepper artefact

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have developed a major problem with the infamous pepper artefact. I have
small intensely black dots associated with the microvilli, rough ER, Golgi,
nuclear envelope, and plasma membrane. The spots vary some in size from
about 6 to 30 nm. They are not on the nucleus or mucin granules except
along their peripheral membranes. Tissues were incubated in vivo with a
biotinylated antibody which would label an apical membrane surface antigen
and then removed and rinsed in 6x in PBS. Fix in 2% PF + 0.2%
glutaraldehyde + 300 nM DAPI in HWB (30 mM HEPES, 70 mM NaCl, 2 mM CaCl2,
pH 7.4) for overnight at 4 C. I need the DAPI to identify the region of
interest prior to dissection. Rinse in HWB + glycine 3 x 10 min. Dissect
out region of interest. Block in 0.1% acetylated BSA for 45 min. Incubate
the samples in a 1:10 dilution of 10 nm gold conjugated goat anti-biotin in
0.1% BSA-c in HWB for overnight at 4 C. Rinse 6x 5 min in HWB. 30 min in
25% ethanol + 0.5% uranyl acetate. 50% , 70%, 95% at 4 C, 3 x 100% ethanol
for 10 min each at -20 °C. 1:1 LR Gold (no catalyst):ethanol 8 hrs at 4
°C. 2:1 LRG (no catalyst):ethanol overnight at 4 C. 100% LRG + catalyst
overnight at -20 °C. 100% LRG + catalyst overnight at -20 °C. 100% LRG +
catalyst for 2 hrs at -20 °C. Embed in 200 ul of fresh LRG +
catalyst in sealed BEEM capsules at 4 C under UV light. Collect ultrathin
sections (50–80 nm) on nickel grids. The problem is present regardless of
whether I use lead or uranyl acetate to stain the thin sections.

I can't remove the pepper by floating the grids on either 0.5% HCl, 1% EDTA
or 2% periodic acid for up to 60 min. I am guessing it is from the uranyl
acetate en bloc but the artefact is small round dots which is not what
uranyl artefacts have looked like in earlier failures. I am confident is
not a gold artefact since gold would not penetrate into the cells and I can
recognize a colloidal gold particle. Any thoughts on where this is coming
from or how to remove it from my existing blocks would be welcome.


Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu




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From: phillipst-at-missouri.edu
Date: Tue, 8 Nov 2005 11:40:38 -0600
Subject: [Microscopy] Quetol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am going to bite the bullet and try another embedding resin for a number
of reasons. I want to give Quetol a try because of its reported nice
contrast and ability to tolerate a small amount of water. The
manufacturers data sheet gives a formulation using volume measurements
(mls) of each component. I must prefer to use the more accurate and
convenient method of weighing the components. Does anyone have a good
Quetol formulation by weight? I googled it and found John Johnson uses:
15 gm Quetol 651
25 gm NSA
4 gm MNA
1 gm DMP-30
but others seem to have quite different proportions. Any comments?



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: twigg-at-estd.nrl.navy.mil
Date: Tue, 8 Nov 2005 11:50:02 -0600
Subject: [Microscopy] Grinding Tinanium Carbide for XTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are trying to prepare XTEM samples from tinanium carbide
substates, but the 180 grit SiC paper we use in the initial grinding
step is just not up to taking on this extremely hard material. Any
suggestions (including vendors for such abrasives)?
--
Dr. Mark E. Twigg
Code 6812
Naval Research Laboratory
4555 Overlook Ave., S.W.
Washington DC 20375

tel: (202) 404-8543
fax: (202) 404-7194

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From: randerson20-at-tampabay.rr.com
Date: Tue, 8 Nov 2005 12:16:27 -0600
Subject: [Microscopy] Re: Grinding Tinanium Carbide for XTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

twigg-at-estd.nrl.navy.mil wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Oh, and patience. Lots of patience.

Ron Anderson


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From: hyi-at-emory.edu
Date: Tue, 8 Nov 2005 12:22:28 -0600
Subject: [Microscopy] Pepper artefact

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Thomas:

If it is not too much trouble, would you send me an image of this
pepper artifact off-line? Thank you in advance.

Hong Yi
Emory EM


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From: edelmare-at-muohio.edu
Date: Tue, 8 Nov 2005 13:16:36 -0600
Subject: [Microscopy] Re: Quetol

Contents Retrieved from Microscopy Listserver Archives
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Thomas:

Here's my formula for Quetol HARD. I've used it numerous times as a
replacment for Spurr's Hard (Quetol version is slightly harder
actually).

Quetol 651 17 g

NMA
(nadic methyl anhydride) 23 g

DMAE / DMP-30
( 2-dimethylaminoethanol or S-1) 0.5 g

=======================================

I am going to bite the bullet and try another embedding resin for a
number
of reasons. I want to give Quetol a try because of its reported nice
contrast and ability to tolerate a small amount of water. The
manufacturers data sheet gives a formulation using volume measurements
(mls) of each component. I must prefer to use the more accurate and
convenient method of weighing the components. Does anyone have a good
Quetol formulation by weight? I googled it and found John Johnson uses:
15 gm Quetol 651
25 gm NSA
4 gm MNA
1 gm DMP-30
but others seem to have quite different proportions. Any comments?



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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Richard E. Edelmann
Electron Microscopy Facility Director
362 Pearson Hall
Miami University
Oxford, OH 45056
Ph: 513-529-5712
Fax: 513-529-4243
HTTP://www.emf.muohio.edu

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From: smalinskas-at-yahoo.com
Date: Tue, 8 Nov 2005 14:29:57 -0600
Subject: [Microscopy] Grinding Tinanium Carbide for XTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Like Ron said, diamond abrasive is the way to go.

Another popular source is Struers (www.struers.com)
and Lapmaster (www.lapmaster.com). They both have
diamond discs (rough, 10-250 micron) and diamond films
(fine, 1/4-30 micron).

Stu Smalinskas, P.E.
Metallurgist
SKF USA
Plymouth, Michigan

--- randerson20-at-tampabay.rr.com wrote:

} Diamond lapping film on mylar substrates. Available
} form a number of
} vendors. We were partial to the films made by DuPont
} because of their
} high density of diamond particles, good particle
} size control, and
} adhesion of the diamonds to the substrate. This
} experience is about five
} years old. I'm sure that by now any number of
} manufacturers can provide
} films with similar properties.
}
} Oh, and patience. Lots of patience.
}
} Ron Anderson
}
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} twigg-at-estd.nrl.navy.mil wrote:
}
}
} } We are trying to prepare XTEM samples from tinanium
} carbide
} } substates, but the 180 grit SiC paper we use in the
} initial grinding
} } step is just not up to taking on this extremely
} hard material. Any
} } suggestions (including vendors for such abrasives)?




__________________________________
Yahoo! FareChase: Search multiple travel sites in one click.
http://farechase.yahoo.com

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From: GBurgess-at-exchange.hsc.mb.ca
Date: Tue, 8 Nov 2005 14:40:40 -0600
Subject: [Microscopy] Printing Digital Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our clinical diagnostic EM laboratory is considering purchasing a digital
camera for our electron microscope to eliminate a lot of the wet lab photo
finishing expenses. I was wondering in the hospital environment how many
people out there end up printing their digital images for records purposes,
rather than just storing the digital images. To me, it seems like a
redundant and expense step if one already has the digital image, since that
image could in fact be printed at any time.

Garry Burgess
Charge Technologist
Health Sciences Centre
Winnipeg, Canada


This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

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From: bozzola-at-siu.edu
Date: Tue, 8 Nov 2005 15:50:55 -0600
Subject: [Microscopy] freeze fracture contractual work needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A colleague in Chemistry would like to have some freeze-fracture work
done on some liposome samples and was wondering if someone would be
available to do this work and the cost per specimen.

Thank you.

JB
--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
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From: TindallR-at-missouri.edu
Date: Tue, 8 Nov 2005 16:37:33 -0600
Subject: [Microscopy] Microtomy: Sorvall knifemaker?

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From: TindallR-at-missouri.edu
Date: Tue, 8 Nov 2005 16:53:37 -0600
Subject: [Microscopy] Microtomy again...

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Sorry, I don't know what happened with the last message, but my request
was whether anyone had or knew of a surplus Sovall glass knifemaker,
circa 1979, for sale. Or does anyone still service these old units?
We need to replace or repair this machine, which takes 3/8" thick
microtomy glass. Alternatively, are there other available knifemakers
out there that will still use the thick glass?

Thanks. This question should be easier to answer, now that it's
actually in the message.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu







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From: Stacey.Andringa-at-uc.edu
Date: Tue, 8 Nov 2005 19:31:47 -0600
Subject: [Microscopy] viaWWW: fading sections

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Question: We have been using a 1% toulidine blue O in 1% sodium borate as a general stain for all of our thick sections (1-2 microns). We use Cytoseal 60 as the mounting medium. We have noticed that the sections are fading more quickly than they have in the past and sometimes we get "round clear droplets" on the sections.
Can anyone recommend a stain that won't fade so quickly?
Does anyone have a clue as to the nature of the
"droplets"?
Thanks.

---------------------------------------------------------------------------

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From: henriks-at-southbaytech.com
Date: Wed, 9 Nov 2005 00:31:38 -0600
Subject: [Microscopy] Re: Grinding Tinanium Carbide for XTEM

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Mark:

Ron is 100% correct in saying that the diamond lapping film would be suitable for this application. Having supplied this material to Ron in the past, I can say with some confidence that he is referring to a product manufactured by 3M rather than by Dupont. I would be pleased to provide you with product details as well as some guidance on your specific application. Please feel free to contact me offline.

Best regards-

David

David Henriks
South Bay Techology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL =1-949-492-2600
FAX: =1-949-92-1499

email: henriks-at-southbaytech.com


--------------------------------------------------------------------------------
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twigg-at-estd.nrl.navy.mil wrote:

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Oh, and patience. Lots of patience.

Ron Anderson

David Henriks
South Bay Techology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL =1-949-492-2600
FAX: =1-949-92-1499

email: henriks-at-southbaytech.com




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From: W.Muss-at-salk.at
Date: Wed, 9 Nov 2005 03:51:23 -0600
Subject: [Microscopy] Re: fading sections

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Good morning all,
dear Mrs Andringa,

the problem with } fading { stains (usually highly alkalinesolutions basic
dyes like Toluidine Blue in borax solution, Azur II-Methylenblue-basic
Fuchsin according to Richardson and others) on plastic (resin) semithin
sections for me is a long known fact (regardless of the mounting media I
used earlier).....so I started some 20 years ago to change my processing
and archiving mode for semithin sections (we have to store the semithins of
our cases for legal reasons -if possible - up to 20 years).

i) for looking at stained sections for orientation purposes, most of them
also for diagnosing, as well as trimming the right location for
EM-ultrathin sections we do not mount them with a slide and/or mounting
medium.
We find the spatial resolution already achieved WITHOUT mounting a
coverslip (compared to a thick histological, paraffin-embedded section) is
enough to choose the location of ultrathin section trimming.

ii) for a necessary photographic documentation or high resolution light
microscopy of } seclected { stained sections we immerse the respective
sections with a small drop of immersion oil, view either with a coverslide
0.17 mm placed carefully on the oildrop then (e.g. for Obj. x 4, x 10, x
25) or directly with a x 50 or x 100 oil immersion objective (Plan, NA=
1,00; 1,30, respectively....those are the good ones from the 60ies and
70ies....).

After having done this, the coverslide will be slipped away, the object
slide will be immersed in (a) coplin jar(s) filled with xylol (2 x 30 sec.
each) or any other diluent which will solve the immersion oil, afterwards
we give the object slides a blast of compressed air (by means of a nozzle
connected by a tube to a } bottle { or the pipeline of compressed air) and
the mounted sections are ready for storing/archiving.
If you do that in a light protected, dustfree place, you will not see - at
least over 5 years from my experience - a fading of staining, also you will
not have "bad wrinkles" in your sections which are produced over time due
to the mounting agent.

This creates the second advantage, the first being economizing the mounting
process (in avoiding permanent mounting with not very healthy mounting
media as well as saving costs for coverslips and the time needed for
coverslipping, drying of mounts, etc.)
IF (in case this happens) the staining of sections has been gone or at
least does not display brilliantly after a long period of time as you are
expecting, you are able to stain again your sections quite easy and
rapidly with the routine staining procedure without loss of substantial
information.

Unfortunately I am really no chemist and therefore I assume a chemical
reaction (de-composing solvent/material due to } aging {) which produces the
"round clear droplets" you see in your mounted sections.

Best regards and wishes for a solution of your problem,

Wolfgang Muss
Salzburg, Austria

PS: if you would like to have some examples of images taken digitally ith
the method(s) given above, I can send them if you like (please send
allowance message for sending them).

----------
Von: Stacey.Andringa-at-uc.edu[SMTP:Stacey.Andringa-at-uc.edu]
Antwort an: Stacey.Andringa-at-uc.edu
Gesendet: Mittwoch, 09. November 2005 02:36
An: W.Muss-at-salk.at
Betreff: [Microscopy] fading sections (Tolblue in Borax)




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Email: Stacey.Andringa-at-uc.edu
Name: Stacey Andringa

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Title-Subject: [Filtered] MListserver:

Question: We have been using a 1% toulidine blue O in 1% sodium borate as a
general stain for all of our thick sections (1-2 microns). We use Cytoseal
60 as the mounting medium. We have noticed that the sections are fading
more quickly than they have in the past and sometimes we get "round clear
droplets" on the sections.
Can anyone recommend a stain that won't fade so quickly?
Does anyone have a clue as to the nature of the
"droplets"?
Thanks.

---------------------------------------------------------------------------

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From: Edward.Calomeni-at-osumc.edu
Date: Wed, 9 Nov 2005 07:42:02 -0600
Subject: [Microscopy] Printing Digital Micrographs

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Garry,

I very rarely print digital images. There are two pathologists who want
prints, so I just give them low resolution, plain paper prints (nothing high
quality) for their own records. All images are stored in a departmental
image database (Paxit). Anyone in our department has access to them.
If images are to be sent out for consultation purposes, I send a CD of the
tif files. In the past 2.5 years, I have printed less than 200 high quality
photo paper digital images.


Ed






Edward P. Calomeni
Director EM Lab
The Ohio State University
M018 Starling Loving Hall
320 W. 10th Ave.
Columbus, OH 43210
614-293-5580 (office)
614-293-8806 (lab)
edward.calomeni-at-osumc.edu
-----Original Message-----
X-from: GBurgess-at-exchange.hsc.mb.ca [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Tuesday, November 08, 2005 3:46 PM
To: Edward Calomeni


Our clinical diagnostic EM laboratory is considering purchasing a digital
camera for our electron microscope to eliminate a lot of the wet lab photo
finishing expenses. I was wondering in the hospital environment how many
people out there end up printing their digital images for records purposes,
rather than just storing the digital images. To me, it seems like a
redundant and expense step if one already has the digital image, since that
image could in fact be printed at any time.

Garry Burgess
Charge Technologist
Health Sciences Centre
Winnipeg, Canada


This e-mail and/or any documents in this transmission is intended for the
address(s) only and may contain legally privileged or confidential
information. Any unauthorized use, disclosure, distribution, copying or
dissemination is strictly prohibited. If you receive this transmission in
error, please notify the sender immediately and return the original.

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From: Mike.Bode-at-soft-imaging.net
Date: Wed, 9 Nov 2005 09:41:26 -0600
Subject: [Microscopy] Printing Digital Micrographs

Contents Retrieved from Microscopy Listserver Archives
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Hello Garry,

We work a lot with Pathology departments in Hospitals, and our experience is that most departments are at first reluctant to the idea of not printing anymore. Prints is what the users are used to and what they know how to interpret. In fact, we often find that the Hospitals have very specific instructions about the size and paper that is to be used for printing. However, once the advantages of digital images hit the users, the use of printed images goes down. Especially, if you can equip all stations with a viewer software and have the ability to archive and transmit images to other facilities, it becomes obvious pretty quickly that prints just take a lot of time and are expensive to ship around. And it is pretty cheap to buy a couple of high quality ink jet printers for the occasional print.

However, in order to get there, you need to plan a bit ahead. Use a software that has an archiving or image database and that allows multiple users to use the archive. Make sure that you have the option to allow external users to access the database. Talk to your IT department. Keep future expansions in mind. Check to see if there are any restrictions (for example, in the US there are HIPAA laws, and the FDA may get involved with something called 21 CFR rule 11).

If you do your homework first, you can set up a system that increases your throughput quite a bit. Let me know if we can help you further with any of this.

Mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
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-----Original Message-----
X-from: GBurgess-at-exchange.hsc.mb.ca [mailto:GBurgess-at-exchange.hsc.mb.ca]
Sent: Tuesday, November 08, 2005 1:43 PM
To: Mike Bode


Our clinical diagnostic EM laboratory is considering purchasing a digital camera for our electron microscope to eliminate a lot of the wet lab photo finishing expenses. I was wondering in the hospital environment how many people out there end up printing their digital images for records purposes, rather than just storing the digital images. To me, it seems like a redundant and expense step if one already has the digital image, since that image could in fact be printed at any time.

Garry Burgess
Charge Technologist
Health Sciences Centre
Winnipeg, Canada


This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.

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From: glenmac-at-u.washington.edu
Date: Wed, 9 Nov 2005 11:19:37 -0600
Subject: [Microscopy] Re: viaWWW: fading sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Stacey,
The droplets are likely water from incomplete dehydration and
clearing. Cytoseal is a toluene based mounting medium that will not
mix with water. Extend your dehydration times and/or add an
additional absolute ethanol step, and maybe add another step in
clearing agent. Make sure you replace the dehydration baths and
clearing baths on a regular basis as water will gradually build up in
them. Although a greater issue with thicker paraffin or vibratome
sections, it can occur with plastic sections if they are rushed
through or the baths have not been changed.

What is the time frame of your fading? Days, months or years? Çould
it be the pH of the Cytoseal? We get a few years with Toluidine Blue
mounted in DPX.

Regards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-u.washington.edu

************************************************************************
******
The box said "Requires Windows 95 or better", so I bought a Macintosh.
************************************************************************
******


On Nov 8, 2005, at 5:33 PM, Stacey.Andringa-at-uc.edu wrote:

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} Question: We have been using a 1% toulidine blue O in 1% sodium
} borate as a general stain for all of our thick sections (1-2
} microns). We use Cytoseal 60 as the mounting medium. We have
} noticed that the sections are fading more quickly than they have in
} the past and sometimes we get "round clear droplets" on the sections.
} Can anyone recommend a stain that won't fade so quickly?
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From: diaspro-at-fisica.unige.it
Date: Thu, 10 Nov 2005 07:51:27 -0600
Subject: [Microscopy] viaWWW: CONFOCAL 7 Meeting

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Email: diaspro-at-fisica.unige.it
Name: Alberto Diaspro

Organization: University of Genoa

Title-Subject: [Filtered] CONFOCAL 7 Meeting

Question: ELAMBS-MICROSCOBIO, DEPARTMENT OF PHYSICS, UNIVERSITY OF GENOA PROUDLY ANNOUNCES THE 7TH PRACTICAL INTENSIVE WORKSHOP ON 3D CONFOCAL MICROSCOPY ENTITLED 3D EXPLORATION OF THE LIVING WORLD

CONFOCAL 7, will be held at LAMBS, GENOA JAN 31-FEb 3, 2006.

KEY SCIENTISTS IN THE FIELD WILL GIVE TALKS ON HOT SUBJECTS IN 3D CONFOCAL MICROSCOPY INCLUDING MULTIPHOTON IMAGING, ìFî TECHNIQUES, BIOMEDICAL APPLICATIONS.

TRAINING WILL BE ON CONFOCAL AND MULTIPHOTON SPECTRAL MICROSCOPES, ULTRAFAST LASER TI-SAPPHIRE LASER SOURCES AND IMAGE DECONVOLUTION WORKSTATIONS. 4D (X-Y-Z-t), FRET, FRAP, FLIM and SHG EXPERIMENTS WILL BE PERFORMED. STUDENTS ARE ENCOURAGED TO BRING THEIR OWN SAMPLES SINCE CELL CULTURE, CHEMICAL, CELL BIOLOGY FACILITY WILL BE AVAILABLE.

Participation fees:

Academia/University Researchers 450 Ä: full School including CD and 2 lunches (Fluorescence basics on demand).

Industry/Private Researchers 600 Ä: full School including CD and 2 lunches

(Fluorescence basics on demand).

Voluntary contribution 50 Ä: Tuesday afternoon Lectures.

FREE: Tuesday afternoon Lectures, for Students, any level, and Junior Researchers.

Practical sessions will be restricted to 20 attendants.

Application: Interested candidates should send an e-mail to the Director of the Course at the following e-mail address: {mailto:diaspro-at-fisica.unige.it} diaspro-at-fisica.unige.it, using the following subject: CONFOCAL 7.

Selection will be realized on a Temporal (first-in-first-out) and Geographical (long distance applicants are favoured) basis. A short Curriculum Vitae and scientific interest of the candidate can be useful for Candidate selection.


For details see EVENTS on www.lambs.it.


---------------------------------------------
[...] Dance with wolves and count the stars, including the
unseen...(L.Ferlinghetti, Challenges to young poet, 2001)
---------------------------------------------
Alberto Diaspro, MicroScoBIO Research Center, LAMBS-IFOM, Department of
Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy
facsimile +39-010314218 - voice +39-0103536426/480/309;
URL: {http://www.lambs.it} http://www.lambs.it
{http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html} http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html
----------------------------------------------

---------------------------------------------------------------------------


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From: Anjeanette.Ormonde-at-unilever.com
Date: Thu, 10 Nov 2005 09:42:49 -0600
Subject: [Microscopy] tweezers search

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was hoping someone out there might be able to help me out. A co-worker (not
a microscopist) brought me a pair of tweezers and wants to order more but can't
remember where they were originally purchased from. She thinks she bought them
from a microscopy catalogue but we haven't been able to find the same ones in
any of the catalogues I typically order from. They are black plastic (she
think they are carbon-fiber) and the primary concern is that they are
anti-static. While we can find plenty of anti-static, plastic tweezers hers
are curved and our searches keep turning up straight or angled pairs. On one
side is the code "J201" and the flip side has a sort of logo. It looks like a
J surrounded by four "pine trees". Any assistance would be greatly appreciated.

Thanks,
Angie




==============================Original Headers==============================
5, 14 -- From Anjeanette.Ormonde-at-unilever.com Thu Nov 10 09:42:43 2005
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5, 14 -- Date: Thu, 10 Nov 2005 10:39:55 -0500
5, 14 -- From: "Anjeanette Ormonde" {Anjeanette.Ormonde-at-unilever.com}
5, 14 -- Subject: tweezers search
5, 14 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
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From: W.Muss-at-salk.at
Date: Thu, 10 Nov 2005 10:04:22 -0600
Subject: [Microscopy] Re: tweezers search

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Angie,

try


http://www.ure.com.my/products/tweezer.html

and find:

J201, curved tips

Hope this helps,

best regards,


Wolfgang Muss
Salzburg, Austria

----------
Von: Anjeanette.Ormonde-at-unilever.com[SMTP:Anjeanette.Ormonde-at-unilever.com]
Antwort an: Anjeanette.Ormonde-at-unilever.com
Gesendet: Donnerstag, 10. November 2005 16:46
An: W.Muss-at-salk.at
Betreff: [Microscopy] tweezers search

------------------------------------------------------------------------
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The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

I was hoping someone out there might be able to help me out. A co-worker
(not
a microscopist) brought me a pair of tweezers and wants to order more but
can't
remember where they were originally purchased from. She thinks she bought
them
from a microscopy catalogue but we haven't been able to find the same ones
in
any of the catalogues I typically order from. They are black plastic (she
think they are carbon-fiber) and the primary concern is that they are
anti-static. While we can find plenty of anti-static, plastic tweezers
hers
are curved and our searches keep turning up straight or angled pairs. On
one
side is the code "J201" and the flip side has a sort of logo. It looks
like a
J surrounded by four "pine trees". Any assistance would be greatly
appreciated.

Thanks,
Angie




==============================Original
Headers==============================
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5, 14 -- Date: Thu, 10 Nov 2005 10:39:55 -0500
5, 14 -- From: "Anjeanette Ormonde" {Anjeanette.Ormonde-at-unilever.com}
5, 14 -- Subject: tweezers search
5, 14 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
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==============================Original Headers==============================
20, 28 -- From W.Muss-at-salk.at Thu Nov 10 10:04:22 2005
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20, 28 -- Subject: [Microscopy] Re: tweezers search
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From: Willem.Wennekes-at-comcast.net
Date: Thu, 10 Nov 2005 17:39:55 -0600
Subject: [Microscopy] AskAMicroscopist: Dry pump emission

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Email: Willem.Wennekes-at-comcast.net
Name: Willem Wennekes

Organization: UES

Education: Graduate College

Location: Dayton, OH, USA

Title: Dry pump emission

Question: Hi, We need to build an exhaust system for our oil pumps. The reason is the carcinogenic nature of the pump oil and therefore the exhaust. Since these pumps are used as backing pumps for systems that do not emit any other hazardous gazes, replacing these pumps with compact dry pumps seems to be a good alternative. However, scroll pumps emit graphite and we do not want to have that in the atmosphere either. I like a piston pump made by Leybold, the Ecodry M, which is advertised to have very low particle emission. Does anybody has experience with this pump or has a suggestion for a zero emission dry pump.

Thanks,

Willem Wennekes

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From: foxglovelj-at-msn.com
Date: Thu, 10 Nov 2005 17:40:39 -0600
Subject: [Microscopy] AskAMicroscopist: 10 year old son who wants a microscope for xmas

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This Question was submitted to Ask-A-Microscopist by (foxglovelj-at-msn.com)
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Email: foxglovelj-at-msn.com
Name: Laura Giard

Education: Undergraduate College

Location: Sterling, MA USA

Title: microscope purchase

Question: Hello, I have a 10 year old son who wants a microscope for xmas. He wants to be a scientist when he grow up! We have purchased "cheap" microscopes over the years and have been very disappointed. I'd like to find one that truly works, however, cannot spend a fortune. Can you give me some help? Magnifications etc. do not really help me in determining the quality which is the only information I can seem to gain from places that sell them. Right now I've been looking at ones through Nasco. ANY HELP would be GREATLY APPRECIATED! Thank you.

---------------------------------------------------------------------------

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From: diller-at-stefan-diller.com
Date: Thu, 10 Nov 2005 17:43:34 -0600
Subject: [Microscopy] viaWWW: KEVEX DELTA plus analyzer

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Email: diller-at-stefan-diller.com
Name: Stefan Diller

Organization: Photography

Title-Subject: [Filtered] MListserver:

Question: Dear all,
does anybody have experience and / or programms to interface a KEVEX DELTA plus analyzer to a modern PC via RS232?
My modell runs the Quantex + software on two Bernoulli 44MB drives.
I have a second Bernoulli set available.
If there is no other soultion, is there a possibility to get the Bernoulli working via SCSI on a PC to exchange the specs / data?

Thanks,
Stefan Diller

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From: gary-at-gaugler.com
Date: Thu, 10 Nov 2005 17:50:22 -0600
Subject: [Microscopy] Re: AskAMicroscopist: Dry pump emission

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would suggest the Edwards XDS5 or XDS10 dry scroll pump
with a BOC Edwards XDS Silencer A50597000. The silencer
is a filter trap on the output. it is available for about
$200. I use both of these pumps and have no problems.

gary g.


At 03:42 PM 11/10/2005, you wrote:



} ----------------------------------------------------------------------------
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From: r.sims-at-auckland.ac.nz
Date: Thu, 10 Nov 2005 18:04:59 -0600
Subject: [Microscopy] re: Dry pump emission

Contents Retrieved from Microscopy Listserver Archives
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Date sent: Thu, 10 Nov 2005 17:41:26 -0600
To: r.sims-at-auckland.ac.nz
X-from: Willem.Wennekes-at-comcast.net
}
} Question: Hi, We need to build an exhaust system for our oil pumps.
} The reason is the carcinogenic nature of the pump oil and therefore
} the exhaust. Since these pumps are used as backing pumps for systems
} that do not emit any other hazardous gazes, replacing these pumps with
} compact dry pumps seems to be a good alternative. However, scroll
} pumps emit graphite and we do not want to have that in the atmosphere
} either. I like a piston pump made by Leybold, the Ecodry M, which is
} advertised to have very low particle emission. Does anybody has
} experience with this pump or has a suggestion for a zero emission dry
} pump.
}


Are you sure the exhaust vapor from a rotary pump is carcinogenic?

Granted, it may not be pleasant or healthy, but as I understand it these oils are just
aliphatic hydrocarbon mineral oils, and they're not carcinogenic, are they?

An oil mist trap can clean up the exhaust to the stage where it will bother no-one,
particularly if discharged to out-of-doors.

Any rotary-pump-oil manufacturers listening? Any well-informed (I'm not) comment on
this?

cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


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From: schooley-at-mcn.org
Date: Thu, 10 Nov 2005 18:24:27 -0600
Subject: [Microscopy] Re: AskAMicroscopist: 10 year old son who wants a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Question: Hello, I have a 10 year old son who wants a microscope
} for xmas. He wants to be a scientist when he grow up! We have
} purchased "cheap" microscopes over the years and have been very
} disappointed. I'd like to find one that truly works, however,
} cannot spend a fortune. Can you give me some help? Magnifications
} etc. do not really help me in determining the quality which is the
} only information I can seem to gain from places that sell them.
} Right now I've been looking at ones through Nasco. ANY HELP would
} be GREATLY APPRECIATED! Thank you.
}
I urge you to look at the advice about buying microscopes on the
MICRO website - URL below. You can start with MICRO's recommended
20x monocular "dissecting scope" (about $75) and go on to a basic
compound scope (about $150) if he is really interested; both are
needed if he's serious. Order the inspirational "Hidden Worlds -
Looking Through a Scientist's Microscope" (less than $5 online), and
one of the several books about simple specimen preparation listed in
the MICRO bibliography, when you get the compound scope. And Email
me directly if you have further questions.

Caroline

--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

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From: tivol-at-caltech.edu
Date: Thu, 10 Nov 2005 18:32:23 -0600
Subject: [Microscopy] Re: AskAMicroscopist: 10 year old son who wants a microscope for xmas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Nov 10, 2005, at 3:40 PM, foxglovelj-at-msn.com wrote:

} Email: foxglovelj-at-msn.com
} Name: Laura Giard
}
} Education: Undergraduate College
}
} Location: Sterling, MA USA
}
} Title: microscope purchase
}
} Question: Hello, I have a 10 year old son who wants a microscope for
} xmas. He wants to be a scientist when he grow up! We have purchased
} "cheap" microscopes over the years and have been very disappointed.
} I'd like to find one that truly works, however, cannot spend a
} fortune. Can you give me some help? Magnifications etc. do not
} really help me in determining the quality which is the only
} information I can seem to gain from places that sell them. Right now
} I've been looking at ones through Nasco. ANY HELP would be GREATLY
} APPRECIATED! Thank you.
}
Dear Laura,
Check out Project MICRO; go to the MSA web site
(www.msa.microscopy.org), click on the Reference & Educational button
on the left side, and then on ProjectMICRO, the 6th item under
Education Committee Functions.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: bozzola-at-siu.edu
Date: Thu, 10 Nov 2005 18:48:59 -0600
Subject: [Microscopy] re: carcinogenicity of dry pump emissions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good question. It is generally accepted that long term irritants
(even at low levels) can be carcinogenic. For example, a jagged edge
of a tooth can cause an oral cancer due to constant irritation. Oil
mists (while not carcinogenic per se) can irritate the lungs and
cause pneumonia or lung inflammation. Organo-metallics (and perhaps
by extension, used pump oils) are known carcinogens. Used motor oil,
for example, since it contains organo-metallics, is considered a low
level carcinogen. I am uncertain (but suspicious, however) if used
synthetic oils are similarly carcinogenic.

Microscopists can spend many hours in a room with a vacuum pump. Over
a period of years, well ...... let's just say that it's worrisome. In
our facility, we exhaust the oil mist from our pumps outside the
building where it condenses on the roof of the building and is
ultimately washed by rain into the soil. Not ideal, but the oil is
diluted by water and can be broken down by microbes over time rather
than being concentrated in one's lungs.

Many years ago, I evaluated an exhaust filter that trapped over 99+%
of the oil mist and returned it to the pump. However, after I placed
a Petri dish with distilled water in the room, I could observe (by
eye as well as under a stereomicroscope) tiny droplets of oil landing
on the water surface and spreading out to form an "oil slick". That's
when I decided to exhaust to the outside.

Maybe the risk of lung cancer is low but why take the chance?

Cheers,

John B.


} Date sent: Thu, 10 Nov 2005 17:41:26 -0600
} To: r.sims-at-auckland.ac.nz
} X-from: Willem.Wennekes-at-comcast.net
} }
} } Question: Hi, We need to build an exhaust system for our oil pumps.
} } The reason is the carcinogenic nature of the pump oil and therefore
} } the exhaust. Since these pumps are used as backing pumps for systems
} } that do not emit any other hazardous gazes, replacing these pumps with
} } compact dry pumps seems to be a good alternative. However, scroll
} } pumps emit graphite and we do not want to have that in the atmosphere
} } either. I like a piston pump made by Leybold, the Ecodry M, which is
} } advertised to have very low particle emission. Does anybody has
} } experience with this pump or has a suggestion for a zero emission dry
} } pump.
} }
}
}
} Are you sure the exhaust vapor from a rotary pump is carcinogenic?
}
} Granted, it may not be pleasant or healthy, but as I understand it
} these oils are just
} aliphatic hydrocarbon mineral oils, and they're not carcinogenic, are they?
}
} An oil mist trap can clean up the exhaust to the stage where it will
} bother no-one,
} particularly if discharged to out-of-doors.
}
} Any rotary-pump-oil manufacturers listening? Any well-informed (I'm
} not) comment on
} this?
}
} cheers
}
} rtch
}
} --
} Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
} Microanalyst Fax : 64 9 3737435
} Department of Geology email : r.sims-at-auckland.ac.nz
} The University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
}
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From: gary-at-gaugler.com
Date: Thu, 10 Nov 2005 18:56:51 -0600
Subject: [Microscopy] Re: AskAMicroscopist: 10 year old son who wants a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You can find some very nice scopes on ebay. Try
for Olympus BH-2/BHT. Look for a unit with trinoc
port and 50W or 100W lamp house. These scopes use
legacy 160mm tube length objectives and can work with
Oly, Nikon, Zeiss, etc. objectives. Over time, you
can work up from Plan objectives to PlanAPO and add
phase and DIC (hard to find). The Zeiss 63X PlanAPO
was probably one of the best 160 objectives they made,
IMO.

This all assumes that you want a compound LM. If you
want a stereo, then look for Oly SH series scopes
or Nikons. These were used extensively in semiconductor
inspection and show up used routinely. But be sure
that the scope has a trinoc port if you ever want to
be able to take pix the best way (versus an ocular).

gary g.


At 03:42 PM 11/10/2005, you wrote:



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From: christopher.hayden-at-novartis.com
Date: Fri, 11 Nov 2005 07:54:43 -0600
Subject: [Microscopy] viaWWW: Eye Fixation Protocol Thank-you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, sure, we want to avoid irritants, but I still would be careful about the "c" word, it
gets tagged on to things so easily, and sticks like glue.

As you say, its the organometallics that make used motor oil a low-level carcinogen,
not the base oil, and good rotary pump oils are just very pure base oils, aren't they?

And who uses synthetic oils in their rotary pumps?

Please note that I'm not advocating exposure to pump exhausts, its just the possibly
careless use of "carcinogenic" that I mind.

cheers

rtch


Date sent: Thu, 10 Nov 2005 18:50:15 -0600
To: r.sims-at-auckland.ac.nz
X-from: bozzola-at-siu.edu
Send reply to: bozzola-at-siu.edu

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Email: christopher.hayden-at-novartis.com
Name: Christopher Hayden

Organization: Novartis Pharmaceuticals

Title-Subject: [Filtered] Eye Fixation Protocol Thank-you

Question: Good Morning, All:

A sincere (and belated) thank-you to everyone who responded to my question about the fixation of mammalian eyes. We ended up going with 6% glutaraldehyde for fixation (one of the many suggested procedures). I'd love to tell everyone how it came out, but our priorities have shifted and it'll be a while before we get to actually scope the samples.

I wanted to write back to everyone indivially to thank them, but due some electronic problems I lost nearly all of my saved email(!), so it's been a fun few weeks trying to get back to "normal". We couldn't have done it as well without everyone's help.

My thanks.
-Chris

--------------
Christopher Hayden
PCS/Electron Microscopy
Novartis Pharmaceuticals
christopher.hayden-at-novartis.com

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==============================Original Headers==============================
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From: simon.ringer-at-emu.usyd.edu.au
Date: Fri, 11 Nov 2005 07:58:07 -0600
Subject: [Microscopy] viaWWW: ACMM19 Conference Deadlines Approaching

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
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Email: simon.ringer-at-emu.usyd.edu.au
Name: Simon Ringer

Organization: University of Sydney

Title-Subject: [Filtered] MListserver: ACMM19

Question: Dear colleague,

I am taking the liberty of contacting you to highlight that the 19th Australian Conference for Microscopy and Microanalysis (ACMM19) will be held in Sydney, Australia, on the 5th to 9th February 2006. The biennial ACMM meetings are one of Australiaís most important forums for the microscopy and microanalytical sciences, covering the gamut of techniques and applications, and attracting a significant international attendance. More information on ACMM19 may be found on the conference web site at

http://www.acmm19.org.au.

I will take this opportunity, however, to draw your attention specifically to three points:
(1) late submissions of abstracts for the conference will be accepted for a few more days;
(2) a late-breaking poster session will be held at the conference; and
(3) the early bird registration closes December 5th.

I, and the rest of the organising committee, look forward to a stimulating scientific interaction with you at ACMM19 in February.

Regards,
Simon

Professor Simon P. Ringer
Convenor, ACMM19

Director
Australian Key Centre for Microscopy & Microanalysis

Executive Director
Nanostructural Analysis Network Organisation
Major National Research Facility
NANO-MNRF
www.nano.org.au

Address:
Electron Microscope Unit
The University of Sydney
NSW, 2006
AUSTRALIA

Contact:
PH) + 61 2 9351 2351
FAX) + 61 2 9351 7682
simon.ringer-at-emu.usyd.edu.au
www.emu.usyd.edu.au

Madsen Building, F09
Room LG21
CRICOS Number: 00026A www.usyd.edu.au/disclaimer.shtml


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From: wesaia-at-iastate.edu
Date: Fri, 11 Nov 2005 08:58:03 -0600
Subject: [Microscopy] Re: viaWWW: KEVEX DELTA plus analyzer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stefan, I have done this in the past, but it might take me a while to dig
out the details. I can give you a quick synopsis now and we can talk more
later.

I have used serial and ethernet communication to transfer data between the
Delta and the rest of the world. I think I tried hooking up a Bernoulli
drive, but then you need a utility that can read the directory under
DOS/Windows. It is not a trivial thing. I received a copy of one, but I
don't know that I ever got it to work.

Serial transfer should work with what you have now. You would only need a
cable to plug between the two systems and maybe some software tweaks. I
don't remember if the Kevex RT-11/TSX+ operating system came with a driver
built in for the serial port. I think it had a driver for RT-11; I don't
quite remember about TSX+. I have rebuilt copies of the operating system
years ago to add different drivers, but it has been a while. Let's suppose
the drivers are built in, the cable should be fairly straightforward. You
may want to find someone with a breakout box to make sure the pins are all
setup correctly. The Kevex doesn't hardware handshaking lines, but I think
the PC does. Finally, you need some software on both ends. I used Kermit
which was a rather ubiquitous program written for lots of platforms. I will
have to see if I still have copies around. It supported both text and
binary transfer. One end should be set up in server mode so the other
computer can push or pull files around.

We went with an ethernet option because serial is slow. I purchased an
ethernet card and software that allowed me to put the PDP onto an ethernet
network. I think the software may only have worked under TSX, but that was
okay because we usually ran under TSX than RT-11 anyway. I think I had to
rebuild the operating system to add in the capability. It might be more
daunting.

The next and primary question will be what will you do with the files once
you have transferred them over? A lot of Kevex stuff was in a proprietary
binary format. I developed procedures for transferring text output.
However, spectra and images are another story. I think Kevex may have
offered a spectrum export option. I developed my own utilities for
converting maps and images over to TIF or BMP format.

So before spending much time hooking the two systems together, what do you
want to do with the files once you can move them?

Warren

At 05:44 PM 11/10/05, you wrote:


} Email: diller-at-stefan-diller.com
} Name: Stefan Diller
}
} Organization: Photography
}
} Title-Subject: [Filtered] MListserver:
}
} Question: Dear all,
} does anybody have experience and / or programms to interface a KEVEX DELTA
} plus analyzer to a modern PC via RS232?
} My modell runs the Quantex + software on two Bernoulli 44MB drives.
} I have a second Bernoulli set available.
} If there is no other soultion, is there a possibility to get the Bernoulli
} working via SCSI on a PC to exchange the specs / data?
}
} Thanks,
} Stefan Diller


==============================Original Headers==============================
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11, 21 -- Subject: Re: [Microscopy] viaWWW: KEVEX DELTA plus analyzer
11, 21 -- Cc: MSA listserver {Microscopy-at-msa.microscopy.com}
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From: Rob.Bowen-at-caddock.com
Date: Fri, 11 Nov 2005 10:02:03 -0600
Subject: [Microscopy] Re: viaWWW: KEVEX DELTA plus analyzer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stefan,
Look at IXRF, http://www.ixrfsystems.com/index.html . They offer
upgrades for Kevex systems and may have something you can use.

No interest in IXRF except as a satisfied user.

Rob Bowen
--
Robert C. Bowen
Research Scientist
Caddock Electronics, Inc
rob.bowen-at-caddock.com
http://www.caddock.com

} From: {diller-at-stefan-diller.com}
} Reply-To: {diller-at-stefan-diller.com}
} Date: Thu, 10 Nov 2005 17:46:35 -0600
} To: {rob.bowen-at-caddock.com}
} Subject: [Microscopy] viaWWW: KEVEX DELTA plus analyzer
}
}
}
}
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} Listserver
} ---------------------------------------------------------------------------
}
} Email: diller-at-stefan-diller.com
} Name: Stefan Diller
}
} Organization: Photography
}
} Title-Subject: [Filtered] MListserver:
}
} Question: Dear all,
} does anybody have experience and / or programms to interface a KEVEX DELTA
} plus analyzer to a modern PC via RS232?
} My modell runs the Quantex + software on two Bernoulli 44MB drives.
} I have a second Bernoulli set available.
} If there is no other soultion, is there a possibility to get the Bernoulli
} working via SCSI on a PC to exchange the specs / data?
}
} Thanks,
} Stefan Diller
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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==============================Original Headers==============================
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From: eschumacher-at-mccrone.com
Date: Fri, 11 Nov 2005 11:22:22 -0600
Subject: [Microscopy] Electron Microscope Identification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings All,

A colleague here has been asked whether we can reproduce work outlined
in a rather
vaguely worded document that describes characterization of hydrated
sections of
film. Photomicrographs are shown that are said to have been acquired
using a
"DEH 345" penetrating electronic microscope. The data bar on the
photomicrographs
has a 6-digit number in the upper left corner that's preceded by the
letters "ES".
We wondered if this might refer to an Electroscan environmental SEM.
Our searches
on the instrument description haven't turned up anything. I'm hoping
that the
collective expertise of the listserver will shed some light on this for
us!

Thank you,

Elaine


Elaine F. Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com




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From: jrunions-at-brookes.ac.uk
Date: Fri, 11 Nov 2005 11:40:40 -0600
Subject: [Microscopy] Planning a Masters course in Bioimaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone (Sorry if you've already seen this on the confocal
microscopy list),

We are in the planning and validation stage of a Masters in Bioimaging
program to be offered at Oxford Brookes University starting in Sep.
2006. This course is supported by the Royal Microscopical Society and
we have gotten good feedback from industry regarding support. I am
seeking input from others who have been involved in or are contemplating
such a course. As part of the validation before the University
committee, we need to show that such a course is viable and that there
would be a reasonable demand for it. The University is doing away with
those smaller Masters programs that only attract a few students per
year. My feeling is that this is no problem with the renaissance in
imaging that confocal microscopy has produced but I need something more
concrete to present to the committee.

Specifically:

1) Are there other Graduate level courses in Bioimaging in the UK or
internationally? If so, how are they doing?
2) What do you feel is the best way to advertise the course, both to new
graduates and to those in industry who might like to take a module or two?
3) If you are a professional in an imaging-related company, what would
you be interested in learning as a module in such a course?

I really want to hear from you whether your views are positive or
otherwise. If you are in the UK and you would like to be involved as a
guest lecturer, sponsor etc., by all means let me know.

Thank you.

Sincerely, John.
--

*********************************
C. John Runions, Ph.D.
School of Biological and Molecular Sciences
Oxford Brookes University
Oxford, UK
OX3 0BP

email: jrunions-at-brookes.ac.uk
phone: +44 (0) 1865 483 964


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From: bozzola-at-siu.edu
Date: Fri, 11 Nov 2005 16:56:38 -0600
Subject: [Microscopy] Refs: carcinogenicity of used pump oil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Used motor oil is considered carcinogenic (see 1 and 2, below).

It appears that short term exposure to oil fumes may not produce
significant adverse health effects according to CITGO (see 2, below).

I can send the full references if you like. Sorry, I didn't have the
time to reformat the quotes. I'll see if I can find any better refs.

Some quotes are enclosed.

1. Brief Summary of Carcinogenicity/Cancer Information:
The 4- to 7-ring PAHs have been especially implicated in the
carcinogenic effect of used oil [519]. Many of these same PAHs are
found in new oil, although in lower concentrations [519]. Above text
reprinted with permission from Environmental Toxicology and
Chemistry, Volume 12(11), C. Upshall, J.F. Payne, and J. Hellou,
"Induction of MFO Enzymes and Production of Bile Metabolites in
Rainbow Trout (Oncorhynchus mykiss) Exposed to Waste Crankcase Oil."
Copyright 1993 SETAC].

The debates on which PAHs and alkyl PAHs in complex mixtures such as
this product to classify as carcinogens, and the details of exactly
how to perform both ecological and human risk assessments on the
complex mixtures of PAHs typically found at contaminated sites, are
likely to continue. There are some clearly wrong ways to go about
it, but defining clearly right ways is more difficult. PAHs usually
occur in complex mixtures rather than alone.

Perhaps the most unambiguous thing that can be said about complex PAH
mixtures is that such mixtures are often carcinogenic and possibly
phototoxic. One way to approach site specific risk assessments would
be to collect the complex mixture of PAHs and other lipophilic
contaminants in a semipermeable membrane device (SPMD, also known as
a fat bag) [894,895,896], retrieve the contaminant mixture from the
SPMD, then test the mixture for carcinogenicity, toxicity, and
phototoxicity (James Huckins, National Biological Service, and Roy
Irwin, National Park Service, personal communication, 1996).
Source: ENVIRONMENTAL CONTAMINANTS ENCYCLOPEDIA OIL, NEW (UNUSED
MOTOR OIL) ENTRY July 1, 1997 COMPILERS/EDITORS: ROY J. IRWIN,
NATIONAL PARK SERVICE WITH ASSISTANCE FROM COLORADO STATE UNIVERSITY
STUDENT ASSISTANT CONTAMINANTS SPECIALISTS: MARK VAN MOUWERIK LYNETTE
STEVENS MARION DUBLER SEESE WENDY BASHAM NATIONAL PARK SERVICE WATER
RESOURCES DIVISIONS, WATER OPERATIONS BRANCH 1201 Oakridge Drive,
Suite 250 FORT COLLINS, COLORADO 80525

2. From the CITGO MSDS (material safety data sheet):
SECTION 3: HAZARDS IDENTIFICATION
Inhalation No significant adverse health effects are expected to
occur upon short-term exposure.
CAUTION: Hot oil can cause thermal burns on contact. "Used" motor oil
has been associated with skin cancer in laboratory animals following
extended contact.

3. From SYNLUBE.COM:
Used Motor Oil, if handled improperly and without proper personal
protection and hygiene is proven to be cancer causing.
Look at any back label of Petroleum or Synthetic Motor Oil made after
1985, and you will find following statement:
"CAUTION: Avoid prolonged or repeated skin contact with used motor
oil. Used motor oil has been shown to cause skin cancer in laboratory
animals. Thoroughly wash exposed areas with soap and water."


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################

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From: sghoshro-at-NMSU.Edu
Date: Fri, 11 Nov 2005 17:29:54 -0600
Subject: [Microscopy] Thank you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi John

Thanks for your detailed reply.

I would be surprised, though, if rotary pump oil, new or used, contains any PAHs, and it
still seems to me that to extend the established carcinogenicity of used engine oil to
rotary vacuum pump oil is a bit of a stretch, isn't it?

Of course it is obviously best to play safe with exposure to any chemical products, but I
don't think we should label something as carcinogenic without fairly good cause.

cheers

rtch


Date sent: Fri, 11 Nov 2005 16:58:07 -0600
To: r.sims-at-auckland.ac.nz
X-from: bozzola-at-siu.edu
Send reply to: bozzola-at-siu.edu

Thanks to everyone who responded to my inquiry regarding fixation of
Hepatitis C infected tissue. We revceived some great responses.

Best wishes,

Soumitra


*************************************************************
Soumitra Ghoshroy
College Associate Professor, Biology
Director, Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3268 (office), 646-3283 (lab)
Fax: 505-646-3282
e-mail:sghoshro-at-nmsu.edu
http://biology-web.nmsu.edu/ghoshroy/ghoshroy.htm
http://emldata.nmsu.edu/eml/

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From: r.sims-at-auckland.ac.nz
Date: Fri, 11 Nov 2005 17:34:18 -0600
Subject: [Microscopy] Regenation of Al2O3 granules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Presumably the Al2O3 granules used in Edwards and other foreline traps can be
regenerated by heating in a furnace.

What temperature and time is necessary to bake/burn off trapped oil without destroying
the physical structure so that Al2O3 dust goes into the rotary pump?

cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


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From: jtwilley-at-sprynet.com
Date: Fri, 11 Nov 2005 20:10:14 -0600
Subject: [Microscopy] Refs: carcinogenicity of used pump oil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Of course there's no way we can make alumina into royalty, I meant "Regeneration"

cheers

rtch


------- Forwarded message follows -------
Date sent: Fri, 11 Nov 2005 17:35:00 -0600
To: r.sims-at-auckland.ac.nz
X-from: r.sims-at-auckland.ac.nz
Send reply to: r.sims-at-auckland.ac.nz

There seems to be a leap from one subject to another here. Used motor oil is just that, motor oil that was used in an internal combustion engine where a thin film of the oil with a very high surface area has been repeatedly exposed to the high temp, high pressure combustion conditions inside the cylinders. That oil is exposed to transitory, highly reactive species and highly poisonous species including free radicals, nitrogen oxides, carbon monoxide, and a host of partially oxidized hydrocarbons and water vapor. Catalytic converters are used downstream to destroy the more troublesome persistent species from the exhaust but the oil absorbs some of them and cycles them back through the cylinders over and over. These species are also free to react with finely divided metal particles and detergent additives in the oil. Its an environment where a plethora of new species are created and, once created, allowed to react with each other. The high pressure also influences the situation by increasing the tendency to force gaseous species into solution in the oil, prolonging their opportunities to react, rather than encouraging their vapor phase dilution.

Whether any chemistry is going on at all in vacuum pump oil depends on what is being exhausted. But it's safe to say that many lab pumps are not run under conditions that make them a pressure reactor like the one that exists in every internal combustion engine.

John Twilley

-----Original Message-----
X-from: bozzola-at-siu.edu
Sent: Nov 11, 2005 5:57 PM
To: jtwilley-at-sprynet.com

Used motor oil is considered carcinogenic (see 1 and 2, below).

It appears that short term exposure to oil fumes may not produce
significant adverse health effects according to CITGO (see 2, below).

I can send the full references if you like. Sorry, I didn't have the
time to reformat the quotes. I'll see if I can find any better refs.

Some quotes are enclosed.

1. Brief Summary of Carcinogenicity/Cancer Information:
The 4- to 7-ring PAHs have been especially implicated in the
carcinogenic effect of used oil [519]. Many of these same PAHs are
found in new oil, although in lower concentrations [519]. Above text
reprinted with permission from Environmental Toxicology and
Chemistry, Volume 12(11), C. Upshall, J.F. Payne, and J. Hellou,
"Induction of MFO Enzymes and Production of Bile Metabolites in
Rainbow Trout (Oncorhynchus mykiss) Exposed to Waste Crankcase Oil."
Copyright 1993 SETAC].

The debates on which PAHs and alkyl PAHs in complex mixtures such as
this product to classify as carcinogens, and the details of exactly
how to perform both ecological and human risk assessments on the
complex mixtures of PAHs typically found at contaminated sites, are
likely to continue. There are some clearly wrong ways to go about
it, but defining clearly right ways is more difficult. PAHs usually
occur in complex mixtures rather than alone.

Perhaps the most unambiguous thing that can be said about complex PAH
mixtures is that such mixtures are often carcinogenic and possibly
phototoxic. One way to approach site specific risk assessments would
be to collect the complex mixture of PAHs and other lipophilic
contaminants in a semipermeable membrane device (SPMD, also known as
a fat bag) [894,895,896], retrieve the contaminant mixture from the
SPMD, then test the mixture for carcinogenicity, toxicity, and
phototoxicity (James Huckins, National Biological Service, and Roy
Irwin, National Park Service, personal communication, 1996).
Source: ENVIRONMENTAL CONTAMINANTS ENCYCLOPEDIA OIL, NEW (UNUSED
MOTOR OIL) ENTRY July 1, 1997 COMPILERS/EDITORS: ROY J. IRWIN,
NATIONAL PARK SERVICE WITH ASSISTANCE FROM COLORADO STATE UNIVERSITY
STUDENT ASSISTANT CONTAMINANTS SPECIALISTS: MARK VAN MOUWERIK LYNETTE
STEVENS MARION DUBLER SEESE WENDY BASHAM NATIONAL PARK SERVICE WATER
RESOURCES DIVISIONS, WATER OPERATIONS BRANCH 1201 Oakridge Drive,
Suite 250 FORT COLLINS, COLORADO 80525

2. From the CITGO MSDS (material safety data sheet):
SECTION 3: HAZARDS IDENTIFICATION
Inhalation No significant adverse health effects are expected to
occur upon short-term exposure.
CAUTION: Hot oil can cause thermal burns on contact. "Used" motor oil
has been associated with skin cancer in laboratory animals following
extended contact.

3. From SYNLUBE.COM:
Used Motor Oil, if handled improperly and without proper personal
protection and hygiene is proven to be cancer causing.
Look at any back label of Petroleum or Synthetic Motor Oil made after
1985, and you will find following statement:
"CAUTION: Avoid prolonged or repeated skin contact with used motor
oil. Used motor oil has been shown to cause skin cancer in laboratory
animals. Thoroughly wash exposed areas with soap and water."


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/~image/
##############################################################

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From: zaluzec-at-microscopy.com
Date: Sat, 12 Nov 2005 08:44:03 -0600
Subject: [Microscopy] Safety/Carcinogenicity of materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

I'll just point out that a good STARTING point when ever you are unsure and
want to find out the generic hazards of a material are the Materials Safety Data Sheets (MSDS).

These are required by virtually all safety departments in most organziations and are
also usually shipped with materials or are available on-line from the manufacturer.

You can also MSDSs on-line at various WWW sites here is a list of
starting points. In general it is a good policy to have access to data sheets
on all chemcials you use in your lab.

http://www.ilpi.com/msds/#What


Most of the sites listed above are free, but a few require registration.

FYI appended is a short excerpt on "Edwards High Vacuum Mechanical Pump Oil"
which is a pretty common mechanical pump oil around EM Labs. You will notice
it says this particular material is not carcinogenitic, although it does not discuss "used" oil.
It does recommend avoiding the mist, which is a good policy.

Having said this, I will concur with others that venting roughing pump systems to
areas outside the immediate laboratory area is generally a good idea. For the most part we
exhaust roughing pumps using simple PVC piping into fume hoods (some of which are
a good distance away ~ 100 ft) the exhaust of those hoods are filtered
and and NOT released into the immediate laboratory air. I prefer this to simple
mist filters that are mounted on the exhaust port of the pumps. Admittedly this
costs more, but it is remember that you or your colleague may end up spending "years"
in a lab and it makes more sense to be cautious rather than cheap.



Nestor
Your Friendly Neighborhood SysOp







EDWARDS HIGH VACUUM INTERNATIO -- H026-00-008 EDWARDS 16 MECHANICAL PUMP OIL
=======================================================
MSDS Safety Information
=======================================================
FSC: 9150
MSDS Date: 08/01/1996
MSDS Num: CHZKX
LIIN: 00F025284
Product ID: H026-00-008 EDWARDS 16 MECHANICAL PUMP OIL

=======================================================
Health Hazards Data
=======================================================
LD50 LC50 Mixture: ORAL LD50(RAT): } 5 ML/KG
Route Of Entry Inds - Inhalation: YES
Skin: NO
Ingestion: YES
Carcinogenicity Inds - NTP: NO
IARC: NO
OSHA: NO
Effects of Exposure: SKIN: DERMATITIS & ERYTHEMA. EYES: IRRITATION OF THE
CONJUNCTIVA. INHALATION: MAY CAUSE A CHRONIC INFLAMMATORY REACTION OF THE
LUNGS & A FORM OF PULMONARY FIBROSIS. INGESTION: ASPIRATION OF LIQUID INT
O THE LUNGS COULD CAUSE PNEUMONIA.
Explanation Of Carcinogenicity: NONE
Signs And Symptions Of Overexposure: DEFATTING, OIL ACNE, OIL FOLICULITIS,
IRRITATION.
First Aid: SKIN/EYES: WASH W/COPIOUS AMOUNTS OF WATER FOR 10 MINS. INHALATION:
REMOVE TO FRESH AIR. INGESTION: DON'T INDUCE VOMITING. SEND TO HOSPITAL
IMMEDIATELY. OBTAIN MEDICAL ATTENTION IN ALL CASES.
=======================================================
Handling and Disposal
=======================================================
Spill Release Procedures: PREVENT ENTRY TO DRAINS & WATERCOURSES. LARGE:
USE A SUITABLE MEDIUM SUCH AS SAND/EARTH. RECLAIM LIQUID DIRECTLY/IN AN
ABSORBENT MEDIUM THEN TRANSFER TO SUITABLE MARKED CONTAINERS & DISPOSE
OF. SMALL: SOAK UP W/SAND/EARTH & DISPOSE OF.
Waste Disposal Methods: DISPOSE OF TO A LICENSED WASTE CONTRACTOR, IAW/FEDERAL,
STATE & LOCAL REGULATIONS.
Handling And Storage Precautions: OIL MIST SHOULD BE KEPT TO A MINIMUM,
PREFERABLY WELL {5 MG/CUM. STORE AWAY FROM DIRECT HEAT.
Other Precautions: AVOID OIL MIST FUMES & VAPORS.
=======================================================
Fire and Explosion Hazard Information
=======================================================
Flash Point Method: PMCC
Flash Point Text: 460.4F
Extinguishing Media: FOAM, DRY POWDER, CO2, HALON.
Fire Fighting Procedures: DON'T USE WATER JETS. WEAR A SCBA IN CONFINED AREAS
& FOR ANY SIGNIFICANT FIRE.
Unusual Fire/Explosion Hazard: AUTOIGNITION TEMP: 608F. THERE IS A POSSIBILITY
OF EXPLOSION IF YOU PUMP GASES CONTAINING OXYGEN AT A CONCENTRATION } 4%
ABOVE THE PROPORTION CONTAINED IN AIR.
=======================================================
Control Measures
=======================================================
Respiratory Protection: NOT REQUIRED UNDER NORMAL CONDITIONS.
Ventilation: LOCAL EXHAUST W/MIST FILTER, EXHAUST TO ATMOSPHERE.
Protective Gloves: IMPERVIOUS POLY VINYL CHLORIDE
Eye Protection: NOT NECESSARY
Other Protective Equipment: OVERALLS.



==================================================================================



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From: gary-at-gaugler.com
Date: Sun, 13 Nov 2005 13:17:11 -0600
Subject: [Microscopy] specimen rotation unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers:

Does anyone know of a rotatable specimen holder
for "standard" pin stubs? What I would like is
a holder to take 3mm pin stubs and be able to
externally rotate it on the main specimen holder.

The application is that the specimen is on a
45 degree pre-tilt holder. Then, I want to be
able to rotate the specimen using an external joystick
or knob. The unit cannot be too tall or it won't fit
through my specimen load lock.

Any ideas?

gary g.


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From: Colin.Veitch-at-csiro.au
Date: Sun, 13 Nov 2005 17:06:07 -0600
Subject: [Microscopy] Hitachi S4300SE/N information

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

It looks like we'll be getting the funds for a new SEM in the near
future (fingers crossed!!) and we are thinking of going down the field
emission, variable pressure, natural, ESEM route.

I have contacts that are using some of the available systems, but there
are no Hitachi S4300SE/N systems in Australia yet. If anyone out there
has had experience with these machines I would appreciate some feedback
on performance, ease of use etc.. Please feel free to email me
personally with any comments.

Just for the record, we are currently using a Hitachi S4100.

Cheers and thank you very much.

Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.



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From: Sally.Stowe-at-anu.edu.au
Date: Sun, 13 Nov 2005 20:05:56 -0600
Subject: [Microscopy] RE: specimen rotation unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm after something similar - a two axis motorised tilt plus rotate device,
must tilt to at least 90 degrees and rotate 360, that can be easily and
reveribly attached to the X-Y motorised stage of an SEM.

Doesn't have to be stunningly precise, does have to be cheap, and could be
designed for any of the standard pin or stub mounts.
cheers
Sally


Dr SJ Stowe
Facility Coordinator
ANU Electron Microscopy Unit
ANU CRICOS#00120C



} -----Original Message-----
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} Sent: Monday, 14 November 2005 6:17 AM
} To: sally.stowe-at-anu.edu.au
} Subject: [Microscopy] specimen rotation unit
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From: gary-at-gaugler.com
Date: Sun, 13 Nov 2005 21:05:46 -0600
Subject: [Microscopy] specimen rotation unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Deben UK has a tilt and rotate unit that is meant to
work like you say. It is not what I am looking for
but may be for you. The posted unit is for JEOL.

http://www.deben.co.uk/details.php?id=17

As a user/consumer, the Deben products are quite good.

gary g.


At 06:08 PM 11/13/2005, you wrote:



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From: ajheim-at-mail.usf.edu
Date: Sun, 13 Nov 2005 23:16:23 -0600
Subject: [Microscopy] viaWWW: Force microscopy on Asylum MFP-3D

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
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Email: ajheim-at-mail.usf.edu
Name: A H

Organization: USF

Title-Subject: [Filtered] Force microscopy on Asylum MFP-3D

Question: Is anyone using the AR MFP-3D to perform aqueous force curves on soft or biological materials?



---------------------------------------------------------------------------

==============================Original Headers==============================
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From: pk-at-aurorasiam.com
Date: Sun, 13 Nov 2005 23:18:29 -0600
Subject: [Microscopy] AskAMicroscopist: What kind of microscope should i get?

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (pk-at-aurorasiam.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, November 13, 2005 at 20:30:40
---------------------------------------------------------------------------

Email: pk-at-aurorasiam.com
Name: Per Kristian

Organization: Asssumption

Education: Graduate College

Location: Thailand

Question: Hi,

I want to get a microscope. My wish is to watch living organisms like viruses and bacteria to see how they behave.

What kind of microscope should i get?

Best regards,

Per Kristian

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From: hagglundk1-at-nku.edu
Date: Mon, 14 Nov 2005 09:24:34 -0600
Subject: [Microscopy] SEM imaging of bacteria encapsulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have a student working on a project in which they are growing
bacterial cultures (pseudomonas and other species) in a hostile
environment. The bacteria form capsules that we are interested in
seeing. The capsules are most likely composed of sugars
(polysaccharides), and we are hoping to find a fixation or other
preparation technique that would help us with imaging these. We have so
far prepared cultures with standard fixation, dehydration, and critical
point drying techniques, but are not sure that we are preserving the
capsules forming around the bacteria.

We do not have a cryo stage available.

Any ideas?

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu


==============================Original Headers==============================
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From: TindallR-at-missouri.edu
Date: Mon, 14 Nov 2005 09:54:13 -0600
Subject: [Microscopy] SEM imaging of bacteria encapsulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You might try using some Alcian blue in the primary fix to stabilise the
capsules.
Works for us for TEM with Campylobacter.

Chris

----- Original Message -----
X-from: {hagglundk1-at-nku.edu}
To: {c.jeffree-at-ed.ac.uk}
Sent: Monday, November 14, 2005 3:25 PM

There are "non-aqueous" fixation techniques and methods involving
ruthenium red and alcian blue that are useful in preserving these
capsules and other biofilms. The first method involves dissolving
osmium tetroxide in a solvent, such as FC-72 from 3M company, rather
than in an aqueous buffer.

For details, see Microscopy Research And Technique 36:390-399 (1997) and
36:422-427 (1997). Also, Biotech Histochem 66(4):173-80 (1991) and J
Comp Pathology 117(2):165-70 (1997).

If you need more references, let me know. I think I have a couple more
somewhere.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu





-----Original Message-----
X-from: hagglundk1-at-nku.edu [mailto:hagglundk1-at-nku.edu]
Sent: Monday, November 14, 2005 9:26 AM
To: Tindall, Randy D.

We have a student working on a project in which they are growing
bacterial cultures (pseudomonas and other species) in a hostile
environment. The bacteria form capsules that we are interested in
seeing. The capsules are most likely composed of sugars
(polysaccharides), and we are hoping to find a fixation or other
preparation technique that would help us with imaging these. We have so
far prepared cultures with standard fixation, dehydration, and critical
point drying techniques, but are not sure that we are preserving the
capsules forming around the bacteria.

We do not have a cryo stage available.

Any ideas?

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu


==============================Original
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From: W.Muss-at-salk.at
Date: Mon, 14 Nov 2005 10:11:53 -0600
Subject: [Microscopy] Re: SEM imaging of bacteria encapsulation

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Hello and Good Morning,

Another proposal:

Tannic acid, LOW molecular weight (that is appr. MW 1600), applied either
in the fixative (0.05 - 0.1 -1.0 %, be sure your glutaraldehyde is of
EM-grade and free of di-, polymers), or fix as you like (buffered as usual,
incl. glutaraldehyde, perhaps higher concentration = {3% to } 6%), wash as
usual, postfix with OsO4 (buffered, 1-2% as usual), start dehydration with
50% EtOH, and immerse then with 1% para-phenylenediamine (PPD, use with
care according to MSDS) in 70% EtOH for at least 30 - 60 min, wash the
specimens several times in pure 70% EtOH (to get rid of non bound PPD) and
go on with dehydration/embedding as usual......

Have not tried acetone as a dehydrating agent, but I don't see a reason why
aceton as a dehydrating agent should fail.....

....... There will be an interesting "difference" in morphology, as
compared to "normal" fixation and dehydration.....(also, you could add a
Tannic acid LOW mol. weight [TA LMW 0.05%-0.1 % hydrous solution, filtered,
finest Millipore or paperfilter) preincubation staining of the ultrathin
sections grids before the classical two-step staining procedure
UO2Ac/Pb-citrate
(TA LMW: 5 min-8 min -at-room temperature, UO2Ac[endvolume 50 ml 1% in EtOH
abs+ adding a drop of acetic acid]15 min, PbCitrate [e.g.
Venable&Coggeshall] 1.5 - 3 min, depending on the freshness of the
Pb-solution)

Best wishes and regards,

Wolfgang Muss
Salzburg, Austria

----------
Von: hagglundk1-at-nku.edu[SMTP:hagglundk1-at-nku.edu]
Antwort an: hagglundk1-at-nku.edu
Gesendet: Montag, 14. November 2005 16:30
An: W.Muss-at-salk.at
Betreff: [Microscopy] SEM imaging of bacteria encapsulation

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We do not have a cryo stage available.

Any ideas?

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu


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From: heckman-at-bgnet.bgsu.edu
Date: Mon, 14 Nov 2005 10:19:40 -0600
Subject: [Microscopy] Fwd: SEM imaging of bacteria encapsulation

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Dear Karl-

Saccharides are seen in very high contrast with the use of ruthenium
red. It is described in some of the older literature by Luft, I
think.
Carol

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From: DFORAN-at-ORA.FDA.GOV
Date: Mon, 14 Nov 2005 10:20:11 -0600
Subject: [Microscopy] FW: Re: AskAMicroscopist: 10 year old son who wants

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Try your local microscope repair service. Often the have refurbished good
to high quality used microscopes.

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On Nov 10, 2005, at 3:40 PM, foxglovelj-at-msn.com wrote:

} Email: foxglovelj-at-msn.com
} Name: Laura Giard
}
} Education: Undergraduate College
}
} Location: Sterling, MA USA
}
} Title: microscope purchase
}
} Question: Hello, I have a 10 year old son who wants a microscope for
} xmas. He wants to be a scientist when he grow up! We have purchased
} "cheap" microscopes over the years and have been very disappointed.
} I'd like to find one that truly works, however, cannot spend a
} fortune. Can you give me some help? Magnifications etc. do not
} really help me in determining the quality which is the only
} information I can seem to gain from places that sell them. Right now
} I've been looking at ones through Nasco. ANY HELP would be GREATLY
} APPRECIATED! Thank you.
}
Dear Laura,
Check out Project MICRO; go to the MSA web site
(www.msa.microscopy.org), click on the Reference & Educational button
on the left side, and then on ProjectMICRO, the 6th item under
Education Committee Functions.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: gwe-at-ufl.edu
Date: Mon, 14 Nov 2005 10:46:08 -0600
Subject: [Microscopy] Re: SEM imaging of bacteria encapsulation

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Freeze drying might be the best bet for holding on the everything
without adding artefactual material such as Ru.

hagglundk1-at-nku.edu wrote:

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From: hyi-at-emory.edu
Date: Mon, 14 Nov 2005 11:56:33 -0600
Subject: [Microscopy] CCD camera for TEM

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Good afternoon, Everyone:
   
We are in the process to get a CCD camera for our TEM. I am not very
familiar with the market. Does anyone have any suggestion in terms of
which companies are reliable and offer good customer service all
that. I remember there was a user gathering a few years ago at MSA
meeting to discuss problems with one particular company. I was not at
that gathering so did not know a lot of details.  Can anyone tell me
what are the issues when buying a CCD camera? 

            Hope to hear your opinions. Thank you in advance.


Hong
Emory EM



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From: tivol-at-caltech.edu
Date: Mon, 14 Nov 2005 12:41:55 -0600
Subject: [Microscopy] Re: SEM imaging of bacteria encapsulation

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On Nov 14, 2005, at 7:24 AM, hagglundk1-at-nku.edu wrote:

} We have a student working on a project in which they are growing
} bacterial cultures (pseudomonas and other species) in a hostile
} environment. The bacteria form capsules that we are interested in
} seeing. The capsules are most likely composed of sugars
} (polysaccharides), and we are hoping to find a fixation or other
} preparation technique that would help us with imaging these. We have
} so
} far prepared cultures with standard fixation, dehydration, and critical
} point drying techniques, but are not sure that we are preserving the
} capsules forming around the bacteria.
}
} We do not have a cryo stage available.
}
Dear Karl,
Two possibilities are negative stain and cryofixation. Even though
you do not have a cryostage, high-pressure freezing followed by
freeze-substitution and embedding will give you the specimen in a block
of resin that can be stained and sectioned in the conventional manner.
Of course, you may not have access to a high-pressure freezer.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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4, 22 -- Subject: Re: [Microscopy] SEM imaging of bacteria encapsulation
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From: PWebster-at-hei.org
Date: Mon, 14 Nov 2005 12:58:27 -0600
Subject: [Microscopy] Re: SEM imaging of bacteria encapsulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Karl,

You might like to try a simplified cryo-fixation method. It is not the best
method for many reasons and may not give you an impressive result as the
high-pressure freezer that Bill Tivol suggests, but it will give you an easy
way into these methods. Hopefully the results will give you enough data for
a grant!

First freeze your bacteria by immersion freezing in as close to the growing
state as possible. If they are growing in suspension, spin them down very
gently and scoop out the pellet to place onto a metal holder (any small
piece of wire or metal that can hold a drop of the suspension will work). If
they are growing on a substrate, leave them there.

Take the bacteria and freeze them by immersion in an efficient cryogen. We
use liquid propane from a bottle we get from a camping store (for cooking
food outdoors) - disclaimer here: propane is inflammable so take suitable
precautions not to blow up the lab. We collect the propane by piping it into
a small plastic cup that is surrounded by liquid nitrogen.

Once the bacteria have been frozen, which is almost immediately after they
have been immersed in the propane, transfer them to vials on dry ice,
containing dry ethanol or acetone. The dry ice is held in a styrofoam box
Leave them on the dry ice for a few days (depending on the size of the
specimen), changing the cold solvent with fresh cold solvent every day.

Transfer the specimens to the fridge (4 degrees) in a small styrofoam box
containing a small amount of dry ice and leave the specimens to warm to 4
degrees. The trick is to let the vials warm up slowly enough to not have the
solvent boil.

Once you have the solvent at 4 degrees, the specimens can be transferred to
a critical point drier and processed for SEM examination.

As I said, this is not the best way to prepare specimens using cryomethods
but you may be surprised by the results you get.

Paul Webster.




Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org





On 11/14/05 7:30 AM, "hagglundk1-at-nku.edu" {hagglundk1-at-nku.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
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} We have a student working on a project in which they are growing
} bacterial cultures (pseudomonas and other species) in a hostile
} environment. The bacteria form capsules that we are interested in
} seeing. The capsules are most likely composed of sugars
} (polysaccharides), and we are hoping to find a fixation or other
} preparation technique that would help us with imaging these. We have so
} far prepared cultures with standard fixation, dehydration, and critical
} point drying techniques, but are not sure that we are preserving the
} capsules forming around the bacteria.
}
} We do not have a cryo stage available.
}
} Any ideas?
}
} Karl Hagglund
} Biological Sciences, SC-102B
} Northern Kentucky University, Nunn Drive
} Highland Heights, KY 41099
} 859-572-5238
} http://semlab.nku.edu
}
}
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20, 20 -- Subject: Re: [Microscopy] SEM imaging of bacteria encapsulation
20, 20 -- From: "Webster, Paul" {PWebster-at-hei.org}
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From: gcc-at-couger.com
Date: Mon, 14 Nov 2005 13:56:14 -0600
Subject: [Microscopy] Re: FW: Re: AskAMicroscopist: 10 year old son who wants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} } Title: microscope purchase
} }
} } Question: Hello, I have a 10 year old son who wants a microscope for
} } xmas. He wants to be a scientist when he grow up! We have purchased
} } "cheap" microscopes over the years and have been very disappointed.
} } I'd like to find one that truly works, however, cannot spend a
} } fortune. Can you give me some help? Magnifications etc. do not
} } really help me in determining the quality which is the only
} } information I can seem to gain from places that sell them. Right now
} } I've been looking at ones through Nasco. ANY HELP would be GREATLY
} } APPRECIATED! Thank you.
} }
}
Dear Laura,

I have a page that I put together for the first time buyer of microscopes
http://www.couger.com/microscope/links/gcnewbuy.html

While I am normally very strongly in favor of buying older used
microscopes in the case of young children it is almost impossible to find
what they need.

If you buy used buy from a dealer if you buy on Ebay research the sellers
feedback and other sales very carefully. There are some good dealers on
ebay but you have to do you home work.

Gordon.


========= From My Page ===
Special Consideration for Children.


Microscopes for children present special considerations. First it is
important that the microscope be an instrument that they use and not play
with a few days and discard. In these days of the Internet and instant
gratification holding my interested is difficult holding a a child's is a
real challenge. Getting a scope too complex for them to use on their own
is a sure way to put most kids off. I know it did me over 50 years ago and
many complex procedures still do.

Another problem is young children's eyes are closer together than most
binocular tubes will close up and their binocular vision not as well
developed so binocular heads may pose a problem for them well into their
teens. Even if the scope will close down enough to accommodate their eyes
the muscular coordination of their eyes may not work with the binocular
head pieces. Having multiple sclerosis I have experienced this and it
quickly causes eyestrain if the eyes do not comfortably lock in on the image.

Of course having eyepieces and other parts that are attached so that they
are difficult to remove and lose or injure the child are important.

It is difficult to find this qualities in a used microscopes so you are
left with little choice but to buy from a dealer in new microscopes. I
hesitate to recommend particular dealer. I don't hesitate to strongly
recommend that you by from someone that has been a microscope dealer for
some years and has a good reputation for marinating the equipment they
sell. Buying from retail stores that have no microscope service department
is no different than buying from ebay except it is easy to find the person
to complain to when things don't work. It is not much easier to get them
fixed in most cases.

For their fist scope a low power 10 to 30 power scope that requires little
or no surface preparation may well hold their interest much longer than a
height power compound scope that require specimen preparation, careful
lighting and scope adjustment to get a decent image. All this is speaking
in generalities there are 6 year old kids that can master a complex scope
just as there are grown men than can't work a magnifying glass.

While I don't recommend any particular seller I use this scope as an
example of a good low power scope for the beginner
http://www.microscopeworld.com/low/lpdis.htm it is a 20x scope that has
all pieces attached so they aren't remove by curious hands and the
monocular set up allow easy use with out constant readjustment among users
and children that have problems with binocular vision have no problems.

Scopes like these http://www.microscopeworld.com/high/hpin.htm are very
good choice for youngsters. Get one with a simple condenser. I allows you
to work with them and the light and condenser actual adjustable stops are
simple enough children can use them unaided. Less expensive used scopes
can be purchased with better lenses but you will be hard pressed to find a
simpler scope than this one. Defused light and simple stop system is more
that adequate and much less frustrating than an adjustable condenser and
iris 40x and lower powered objectives. The contrast suffers a little at
40x but it is still good enough for very good viewing. Actual test
surprised me.

If the child has a real interest in science I would let him try some more
complex scopes and see what he can handle and try to come to a realistic
conclusion of his desires, abilities and drive to complete projects before
getting a complex microscope.



If you are considering a microscope for a gift to a child consider the
level of complexity the child can deal with. Condensers can give them a
hard time so get one with a simple condenser system or be prepared to
spend a lot of time with them if you get a complex one. Also binocular
heads are a problem. They may not close to the point that they can use
them and until their middle teens there binocular vision may not develop
to be able to use them very well.

In used compound scopes I have used the AO Spencer 160 with very good
results.

For the younger child I have found nothing better than the this Asian
import. http://www.microscopeworld.com/low/lpdis.htm

Occasionally a monocular dissection scope comes up on ebay but not often.
These scopes take little or no preparation of specials and are less likely
to end up in the closet if the child finds things to complex. For a
binocular scope AO Spencer Cycloptics sell for $ 50 and up on ebay and
www.roseoptics.com has some serviced and rebuilt ones that have the prisms
attached with modern cement that he sells for a reasonable price with a
guarantee. These old Cycloptics are ideal for children because they are so
sturdy and almost any one can clean sand dirt out of them if they
children get careless. and they offer 7 to 25x or 14 to 50x with a 2x lens.


==============================Original Headers==============================
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From: lkrupp-at-us.ibm.com
Date: Mon, 14 Nov 2005 14:11:38 -0600
Subject: [Microscopy] TEM sample storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello-

I was wondering how other listers store their TEM samples between
preparation and microscope time. We have oxidation problems, and I am
curious whether it is better to store samples under vacuum or an inert
gas, as far as preventing/delaying oxidation. Right now we store ours in
a cabinet pumped out by a diaphragm pump, and we see contamination even
after a couple days on our sensitive samples.

Thank you,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099

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From: m.obrien-at-sgul.ac.uk
Date: Mon, 14 Nov 2005 15:02:49 -0600
Subject: [Microscopy] viaWWW: help with Colorview12 camera and GrabBit card.

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
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Email: m.obrien-at-sgul.ac.uk
Name: Marc O'Brien

Organization: St George's University of London

Title-Subject: [Filtered] help with Colorview12 camera and GrabBit card.

Question: Hi

We have an Olympus BX51 with a Soft Imaging Systems
framegrabber camera and software, we've tried
upgrading the PC but have found that PCI GrabBit
card is not recognised by the motherboard. SIS say
they can upgrade the card for around 800 euros, but
can't supply a loan card while the GrabBit is away
being upgraded. They also state that the colorview
12 camera will not work with any other framegrabber
card .

We are loathe to dismantle our only working system.

Our only option would seem to be buying or borrowing
a spare PCI grabBit, and then having the upgrade
done, thus keeping a working system.

Does anyone have one of these cards spare?

Does anyone know of other framegrabber cards that
work with the colorview12 camera and Analysis 5
software?

Has anyone been through this process with SIS and
have any comments.

Any other thoughts or comments gratefully received.

Thanks

Marc O'Brien

---------------------------------------------------------------------------

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From: r.sims-at-auckland.ac.nz
Date: Mon, 14 Nov 2005 15:13:16 -0600
Subject: Re: [Microscopy] re: Dry pump emission

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steve

Yes, I'm sure that you are correct, but I STILL don't think that the term "carcinogenic"
should be used lightly, and without some foundation.

We're supposed to be scientists, aren't we?

We can't just call all nasties "carcinogenic" just because they're unhealthy..

cheers

rtch



Send reply to: "Steve Chapman" {protrain-at-emcourses.com}
X-from: "Steve Chapman" {protrain-at-emcourses.com}
To: {r.sims-at-auckland.ac.nz}



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From: papalia-at-udel.edu
Date: Mon, 14 Nov 2005 15:14:52 -0600
Subject: [Microscopy] Re: TEM sample storage

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Hi Leslie,

We've had some success in preserving easily oxidized samples by using
a rotary vane vacuum pump, grade 5 argon, and a vacuum
desiccator. We just put the samples in the desiccator (with or
without desiccant - your choice for your application), then triple
purge it with vacuum & argon, ultimately leaving it under active
vacuum. When ready for the samples, back-filling with the argon
again will buy you enough time to open up the desiccator and remove
your sample. We've seen a definite difference between the use of
nitrogen and the use of argon with argon being better at displacing
the oxygen in the system.

Hope that helps,
John

} Hello-
}
} I was wondering how other listers store their TEM samples between
} preparation and microscope time. We have oxidation problems, and I am
} curious whether it is better to store samples under vacuum or an inert
} gas, as far as preventing/delaying oxidation. Right now we store ours in
} a cabinet pumped out by a diaphragm pump, and we see contamination even
} after a couple days on our sensitive samples.
}
} Thank you,
} Leslie
}
} Leslie Krupp (Thompson)
} IBM Almaden Research
} 650 Harry Road, K19/D2
} San Jose, CA 95120-6099

John Papalia
Galvin Research Group
201 duPont Hall
Dept. of Materials Science & Engineering
University of Delaware
Newark, DE 19716



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From: schooley-at-mcn.org
Date: Mon, 14 Nov 2005 19:37:27 -0600
Subject: [Microscopy] Re: AskAMicroscopist: What kind of microscope should

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Per - You're being a bit too ambitious; start with larger organisms.
No one looks at living viruses; they're so small that an electron
microscope is needed. Dead & stained bacteria can be seen with a
compound microscope. If you want some fun & action, start with
protozoa in pond water. Look at the MICRO bibliography & order a
book or two. "Explore the world using protozoa" &/or "Guide to
microlife", and "Exploring with the microscope". Start with one of
the many Chinese scopes, perhaps with LED illumination. Get one that
you can add an 100x oil immersion objective to, AFTER you learn how
to use it. Then you'll be able to see bacteria, but they aren't
going to be very exciting to watch.
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

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From: alex.titkov-at-millenniumchem.com
Date: Tue, 15 Nov 2005 00:55:20 -0600
Subject: [Microscopy] INCA Energy software

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Can anyone please tell me what the latest issue of The Microanalysis Suite
from Oxford Instruments is currently available?
What issue are you using? We are on issue 13. Our service contract entitles
us for free software upgrades, but we just can not get the information from
the local Oxford representatives.

Many thanks,
Alexander Titkov

Millennium Inorganic Chemicals Ltd
A Lyondell Company






Lyondell Chemical Company

The information contained in this e-mail message and any attachments may be confidential. It is intended only for the use of the individual or entities named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail at the originating address.

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From: ron.doole-at-materials.ox.ac.uk
Date: Tue, 15 Nov 2005 02:14:09 -0600
Subject: [Microscopy] INCA Energy software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Alex,

I am running Issue 15.

If you are having problems locally contact the Head Office in UK. They have
always been very helpful to me.

Regards,
Ron

-----Original Message-----
X-from: alex.titkov-at-millenniumchem.com [mailto:alex.titkov-at-millenniumchem.com]

Sent: 15 November 2005 07:04
To: ron.doole-at-materials.ox.ac.uk

Hi,

Can anyone please tell me what the latest issue of The Microanalysis Suite
from Oxford Instruments is currently available?
What issue are you using? We are on issue 13. Our service contract entitles
us for free software upgrades, but we just can not get the information from
the local Oxford representatives.

Many thanks,
Alexander Titkov

Millennium Inorganic Chemicals Ltd
A Lyondell Company






Lyondell Chemical Company

The information contained in this e-mail message and any attachments may be
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originating address.

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From: gary-at-gaugler.com
Date: Tue, 15 Nov 2005 02:16:02 -0600
Subject: [Microscopy] Re: viaWWW: help with Colorview12 camera and

Contents Retrieved from Microscopy Listserver Archives
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I have a GrabBit PCI card you can borrow. $20 and a
promise to return it. You pay for shipping out and back.
$995 if you want to keep it.


gary g.



At 01:04 PM 11/14/2005, you wrote:



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From: kunli218-at-yahoo.com
Date: Tue, 15 Nov 2005 07:48:15 -0600
Subject: [Microscopy] viaWWW: Plan view TEM sample prep contamination

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Email: kunli218-at-yahoo.com
Name: Simon Lee

Organization: CharteredSEMi

Title-Subject: [Filtered] MListserver:Plan view TEM sample prep contamination

Question: Dear Listers,

We met some contamination problem when preparing plan-view TEM sample where the sample can only be milled from one side. We found that the contamination at the unmilled side is quite significant (redeposition). The miller we use is PIPS from Gatan. Will the graphite post help? and Is there any other procedure to minimize the contamination?

Thanks in advance for your help.

Rdgs,

Simon

Chartered Semiconductor

---------------------------------------------------------------------------

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From: U.J.Potter-at-bath.ac.uk
Date: Tue, 15 Nov 2005 07:55:05 -0600
Subject: [Microscopy] viaWWW: Resin/wax sectioning problems

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Email: U.J.Potter-at-bath.ac.uk
Name: Ursula Potter

Organization: The University of Bath UK

Title-Subject: [Filtered] MListserver: Resin/wax sectioning problems

Question: Dear All,

We have a problem with the sectioning of Zebrafish embryos (early stages with yolk sac). The embryos have been subjected to an in situ hybridization staining method and when subsequently embedded in wax or resin (technovit) the sections show very bad chatter (wax) or tearing (resin) at the area of the yolk sac. Has anyone experienced this problem? Advice to solve problem would be gratefully received.

Thanks
Ursula

---------------------------------------------------------------------------

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From: eschumacher-at-mccrone.com
Date: Tue, 15 Nov 2005 08:03:20 -0600
Subject: [Microscopy] INCA Energy software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Alexander,

I just received Issue 16 last week as a service contract upgrade. We've
always gotten prompt responses on software and other issues from our
local service and sales reps and from the MA office, with additional
support from the UK if necessary. Getting the upgrade with your service
contract renewal should be straightforward.

Regards,

Elaine

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
*********************************************************************

-----Original Message-----
X-from: alex.titkov-at-millenniumchem.com
[mailto:alex.titkov-at-millenniumchem.com]
Sent: Tuesday, November 15, 2005 12:56 AM
To: Elaine F. Schumacher

Hi,

Can anyone please tell me what the latest issue of The Microanalysis
Suite
from Oxford Instruments is currently available?
What issue are you using? We are on issue 13. Our service contract
entitles
us for free software upgrades, but we just can not get the information
from
the local Oxford representatives.

Many thanks,
Alexander Titkov

Millennium Inorganic Chemicals Ltd
A Lyondell Company






Lyondell Chemical Company

The information contained in this e-mail message and any attachments may
be confidential. It is intended only for the use of the individual or
entities named above. If the reader of this message is not the intended
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or copying of this communication is strictly prohibited. If you have
received this communication in error, please notify us immediately by
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From: hagglundk1-at-nku.edu
Date: Tue, 15 Nov 2005 09:02:32 -0600
Subject: [Microscopy] Imaging bacteria capsules Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to everyone who responded to my question about imaging bacteria.
We have a freeze dryer available, and will likely attempt using this
first. The best part is that our student has plenty of directions to
continue her research.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu


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From: richard.beanland-at-bookham.com
Date: Tue, 15 Nov 2005 09:18:31 -0600
Subject: [Microscopy] viaWWW: Plan view TEM sample prep contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Simon,
I have a PIPS which I use for cross sections and the occasional plan view. I find it is not too bad, but I usually have to give the original surface a tickle with the ion beam to clean off contamination. I generally use the standard double-sided holder, not the graphite post (since I have never been very confident about getting the sample off the post again without breaking it!) Milling conditions are double modulation, 3 degrees incidence from below with the top surface up (i.e. facing the viewing port), slowest possible rotation, 6kV. When the sample is thin enough I turn the voltage down to 2.5 kV (the gas flow has to be adjusted to get maximum current) to give a final clean of the milled surface for a minute or two. The top surface clean is also at 2.5 kV but only one burst of the gun, probably about 12 seconds. This has worked quite well, I have identified nm scale contamination on SiO2 films without any real problems with artefacts. It probably helps to stop milling as soon as you get the smallest hole.
If I really have to keep the top surface intact I prefer using chemical methods, jet etching with Cl in methanol for III-Vs or HF:HNO3 for Si.

Good luck!

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


-----Original Message-----
X-from: kunli218-at-yahoo.com [mailto:kunli218-at-yahoo.com]
Sent: 15 November 2005 13:50
To: Richard Beanland

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
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Email: kunli218-at-yahoo.com
Name: Simon Lee

Organization: CharteredSEMi

Title-Subject: [Filtered] MListserver:Plan view TEM sample prep contamination

Question: Dear Listers,

We met some contamination problem when preparing plan-view TEM sample where the sample can only be milled from one side. We found that the contamination at the unmilled side is quite significant (redeposition). The miller we use is PIPS from Gatan. Will the graphite post help? and Is there any other procedure to minimize the contamination?

Thanks in advance for your help.

Rdgs,

Simon

Chartered Semiconductor

---------------------------------------------------------------------------

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From: lkrupp-at-us.ibm.com
Date: Tue, 15 Nov 2005 09:39:35 -0600
Subject: [Microscopy] Re: viaWWW: Plan view TEM sample prep contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Simon-

For plan view samples I prefer to use the regular post sample holder. I
attach the sample with a tiny drop of crystal bond (I mean tiny!), which
then gets cleaned off manually with a pointed q-tip and acetone. I do not
like to soak off the samples because I feel it contaminates the area you
just milled. Also, stop milling when you get the smallest possible hole
or it will redep through the hole, and reduce the kV as you get closer to
finishing.

If crystal bond residue cannot be tolerated (of course you can't clean it
all off), then I use the graphite holder, with a 3mm disc cut from a glass
coverslip underneath the sample to protect the surface. The only problem
with the graphite holder is the slider bars on either side will block the
beam when using rotation only, and the hole will end up somewhat oblong.

If none of that works, you can mill the sample for a few seconds on the
'good' side, but this is hit or miss.

Sincerely,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099







Email: kunli218-at-yahoo.com
Name: Simon Lee

Organization: CharteredSEMi

Title-Subject: [Filtered] MListserver:Plan view TEM sample prep
contamination

Question: Dear Listers,

We met some contamination problem when preparing plan-view TEM sample
where the sample can only be milled from one side. We found that the
contamination at the unmilled side is quite significant (redeposition).
The miller we use is PIPS from Gatan. Will the graphite post help? and Is
there any other procedure to minimize the contamination?

Thanks in advance for your help.

Rdgs,

Simon

Chartered Semiconductor

---------------------------------------------------------------------------

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From: john.mardinly-at-intel.com
Date: Tue, 15 Nov 2005 10:40:25 -0600
Subject: [Microscopy] TEM sample storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We use the Gatan 655 dry pump station with the TEM specimen holder
accessory. It is a bit pricy, but does an outstanding job, and it is
very quick and easy to use, with cycle time measured in seconds!
http://www.gatan.com/pdf/655%20Dry%20Pumping%20Station.pdf


John Mardinly
Intel Corporation

The opinions of this writer do not necessarily represent the opinion of
Intel Corporation.


-----Original Message-----
X-from: lkrupp-at-us.ibm.com [mailto:lkrupp-at-us.ibm.com]
Sent: Monday, November 14, 2005 12:12 PM
To: Mardinly, John

Hello-

I was wondering how other listers store their TEM samples between
preparation and microscope time. We have oxidation problems, and I am
curious whether it is better to store samples under vacuum or an inert
gas, as far as preventing/delaying oxidation. Right now we store ours
in
a cabinet pumped out by a diaphragm pump, and we see contamination even
after a couple days on our sensitive samples.

Thank you,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099

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From: bozhilov-at-ucr.edu
Date: Tue, 15 Nov 2005 10:41:02 -0600
Subject: [Microscopy] EDAX Genesis 3.6 Net intensity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I need to verify what do the Net Intensity numbers in the
quantification panel represent in the MThin version of the EDAX
Genesis 3.6 software.

According to the manuals: "Net Intensity is the Intensity of the peak
minus the background after de-convolution".

The problem I have is that using the value of the measured net
intensity listed after quantification, I cannot obtain the same
number for the Intensity error as listed by the software. I am using
the formula:
square root of (net intensity multiplied by the live seconds) divided
by the
(net intensity multiplied by the live seconds).

If I subtract the value of the intensity of the background from the
Net Intensity value then I get exactly the number listed as intensity
error in the software.

The question is now. Which is correct? The statement that the Net
intensity are peak intensity minus the background or that the Net
Intensity is the peak intensity + the background. Or may be I am not
using the correct calculation procedure for the intensity error?

I have sent this question to EDAX representatives about three weeks
ago and I am still waiting for an answer so I decide to "pool the
audience" as Regis Philbin likes put it.

_______________________________________
Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel 951 927 2998
fax 951 827 2489
bozhilov-at-ucr.edu
_______________________________________



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From: walck-at-southbaytech.com
Date: Tue, 15 Nov 2005 12:11:01 -0600
Subject: [Microscopy] viaWWW: Plan view TEM sample prep contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There are a couple of ways to prevent contamination on the back side
during ion milling of a plan view sample. The most common way is to use
a lacquer that your remove afterwards. The one that I would recommend
is MicroShield or Microstop -I can't remember the name for sure and it
is available from SPI as well as the remover. Other people have used
nail polish. May I recommend Sally Hanson's Hard As Nails?

I can't find the reference for the neatest way that I think is the way
to do this. I thought that it was in the first MRS TEM sample Prep book
(Vol 115), but I couldn't find it there. What you do is evaporate NaCl
in a vacuum evaporator onto the side that you want to protect. Ion mill
on the other side. The NaCl layer is the one that is contaminated and
after perforation in the ion mill, you simply dip the sample in
distilled water to dissolve the NaCl and float off the contamination
layer. I wish that I could remember who did that, but I thought that it
was a pretty slick idea. If anyone knows the reference or who did it,
please let me know because I would like to put that in our application
notes section of our web site.


-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: kunli218-at-yahoo.com [mailto:kunli218-at-yahoo.com]
Sent: Tuesday, November 15, 2005 5:53 AM
To: Walck-at-SouthBayTech.com

This Question/Comment was submitted to the Microscopy Listserver using
the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
------------------------------------------------------------------------
---
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both kunli218-at-yahoo.com as well as the MIcroscopy
Listserver
------------------------------------------------------------------------
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Email: kunli218-at-yahoo.com
Name: Simon Lee

Organization: CharteredSEMi

Title-Subject: [Filtered] MListserver:Plan view TEM sample prep
contamination

Question: Dear Listers,

We met some contamination problem when preparing plan-view TEM sample
where the sample can only be milled from one side. We found that the
contamination at the unmilled side is quite significant (redeposition).
The miller we use is PIPS from Gatan. Will the graphite post help? and
Is there any other procedure to minimize the contamination?

Thanks in advance for your help.

Rdgs,

Simon

Chartered Semiconductor

------------------------------------------------------------------------
---

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From: frank.karl-at-degussa.com
Date: Tue, 15 Nov 2005 13:04:53 -0600
Subject: [Microscopy] in search of training....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just had a EDS system installed on my TEM. As I'm still learning how to
use the TEM side of this equation, does anyone have any suggestions on
short courses or training I can get so I know what I'm doing?

Thanks in advance!!!

Frank Karl
Degussa Corporation
Akron Technical Center
3500 Embassy Parkway
Suite 100
Akron, Ohio 44333


330-668-2235 Ext. 238


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From: mgb-at-ansto.gov.au
Date: Tue, 15 Nov 2005 16:36:33 -0600
Subject: [Microscopy] GIF collection semi-angle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

A colleague asked me to post the following question. We would
appreciate any feedback either directly to me or to the Listserver.
Cheers,

"Does any one know the value for GIF collection semiangle for a JEOL
2010F /GIF 2000 (200kV) when operated with an image on the screen, no
objective aperture present and a 3mm GIF entrance aperture?

If you have a value, I'd also appreciate the physical dimensions,
assumptions and calculations you have made in arriving at it?

Many thanks in advance."


Mark Blackford
Institute of Materials and Engineering Science, ANSTO
PMB 1,
Menai, N.S.W., 2234
Australia

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily
represent the official views of ANSTO from which this message was
conveyed.




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From: walck-at-southbaytech.com
Date: Tue, 15 Nov 2005 17:05:47 -0600
Subject: [Microscopy] TEM sample storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am not sure how to write this so that it does not sound like too much
of a commercial. I will put the disclaimer up front so that you can
choose to read on and I will try to be as factual about the product as I
can without too much commercialism and hope that I don't get spanked by
Nestor. I think that I am within the guidelines because what I am
relating below is the design considerations and features that evolved
out of solving the solution to my microscopy studies that I have not
reported on before.

Disclaimer: South Bay Technology manufactures and sells the
SampleSaver(TM) Portable Storage Container that addresses the problem of
transportation and storage of samples or samples with coatings that
react when exposed to the atmosphere. This product was just introduced
at the M&M 2005 meeting and other than that and the recent ISFTA 2005
meeting, we have not advertised it. In response to this question, we
just put the brochure for the unit on our web site in order so that
anyone interested can view what the unit looks like. This unit was
specifically made to address the general topic of this question.

The SampleSaver(TM) portable storage container (SS) is my first
contribution to the SBT product line and came about because of problems
that I had with reactive samples when I worked at Wright Patterson Air
Force Base and at PPG Industries, Inc. It is a plastic container in
which there are two valves, one of which is a conventional valve and one
which is integral into the body of the unit, itself. Metallurgical,
SEM, and TEM samples can be held inside with different "sample trees".
There is also a special unit that we have that is made for FIB lift-out
samples. The SS is designed to be purged without pumping it down with
an inert gas that could be N2, Ar, or CO2 from a cylinder or from a
special unit, which we also sell, will use the boil-off from liquid
nitrogen. The idea of using the boil-off from liquid nitrogen (or the
CO2 gas from Dry Ice) is that if you prepare your samples elsewhere and
travel to another lab to do the analysis or vice versa (e.g. when you
FIB your samples at another lab and bring them home) the other lab will
always have liquid nitrogen available to use as the source for the inert
gas. They may or may not have a vacuum pump, Ar line, or a N2 line
available for use. One major benefit of using LN2, is that the boil-off
from LN2 is extremely pure. When the SS unit is sufficiently purged,
the two valves are closed, body valve first then the gas supply valve.
The valve body can then be compressed. This then pressurizes the
container to help prevent ingress of any gas species by diffusion
through the plastic unit. The unit can be tested for integrity just
before opening by opening the valve while it is compressed. A "psst"
sound is reassuring. A tube from the valve can be put under LN2 so that
the container is not exposed to air.

I have images of XTEM samples of Low-E coatings on glass that have been
preserved using this system that I could share with anyone that is
interested. These Low-E coatings contain two layers of silver that when
the two surfaces of the cross section are exposed degrade in air over a
short period of time. Samples that have been stored for many weeks have
not undergone any degradation.

The unit was also developed for SEM samples that would be coated with
chromium where the samples would otherwise oxidize in a relative short
period. It was also intended for EBSD applications of metallurgical
materials where surface oxidation after preparation would degrade the
EBSD results.

Although I designed this only to be used in a purging mode because a
vacuum pump would be too bulky for portability and you can't guarantee
that one is available at the other lab, it can also be used with a
vacuum pump, if desired, and a pump-backfill sequence is possible with
it. I don't recommend leaving it in a vacuum condition, because of the
possible diffusion of species such as O2 and H2 through the plastic.
The positive pressure within the SS with an inert gas inhibits this
diffusion.

The SS can also be used with glove boxes such as the type that was
introduced by Omniprobe at M&M 2005. Here, the unit is simply put
inside the glove box and when the glove box is sufficiently purged,
samples can be put into it or removed from it and then reclosed. By
compressing the body, the pressure inside the SS will be higher than the
pressure in the glove box.

What we don't know at this time is whether samples stored in it and used
with it will require plasma cleaning for high resolution work. My
results have been good and I have not seen any contamination when used
with a LaB6 microscope. I have transported samples to field emission
microscopes and have not seen contamination issues. I suspect that the
higher pressure of the inert gas inside the SS may prevent or inhibit
any out gassing from the plastic body, but I am only guessing here.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: lkrupp-at-us.ibm.com [mailto:lkrupp-at-us.ibm.com]
Sent: Monday, November 14, 2005 12:15 PM
To: Walck-at-SouthBayTech.com

Hello-

I was wondering how other listers store their TEM samples between
preparation and microscope time. We have oxidation problems, and I am
curious whether it is better to store samples under vacuum or an inert
gas, as far as preventing/delaying oxidation. Right now we store ours
in
a cabinet pumped out by a diaphragm pump, and we see contamination even
after a couple days on our sensitive samples.

Thank you,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099

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From: rcommon-at-msu.edu
Date: Tue, 15 Nov 2005 18:01:37 -0600
Subject: [Microscopy] Fading Sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

After discovering that many of our older toluidine blue stained semi-thin
sections that had been mounted with a commercial mounting medium had faded,
I decided to try using our standard Epon-Araldite-DDSA embedding resin as a
mounting medium. Simply put a drop or two of resin (we use left over resin
from embeddings, stored in vials in a freezer) on the dry, stained slides,
and coverslip slowly to avoid bubbles. The slides are viewable immediately,
and will harden in a few days on their own, or overnight at 60 degrees.
They should not be overheated or some destaining or wrinkling may occur.
Since switching to Epon as a mounting medium, I have not seen any problem
with fading.

As for viewing slides before permanent mounting, the image can be greatly
improved just by placing a coverslip over the dry section while viewing.
Most objectives are corrected for the presence of a coverslip, so even with
nothing but air between the coverslip and the section, the image is very
good.

Ralph Common
Division of Human Pathology
Michigan State University


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From: alex.titkov-at-millenniumchem.com
Date: Tue, 15 Nov 2005 21:34:09 -0600
Subject: [Microscopy] INCA Energy software

Contents Retrieved from Microscopy Listserver Archives
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Thanks everyone. I shall chase issue 16 then. Forgot to mention that we are
in Australia, not in the States as some people thought.

Cheers,
Alex

==================
Alexander Titkov
Millennium Inorganic Chemicals Ltd
A Lyondell Company
Locked Bag 245 Bunbury WA 6230
AUSTRALIA
Ph 08 9780 8505
FAX 08 9780 8500
E-mail: alex.titkov-at-millenniumchem.com




eschumacher-at-mccro
ne.com To: alex.titkov-at-millenniumchem.com
cc:
15/11/2005 10:06 Subject: [Microscopy] RE: INCA Energy software
PM
Please respond to
eschumacher









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Hi Alexander,

I just received Issue 16 last week as a service contract upgrade. We've
always gotten prompt responses on software and other issues from our
local service and sales reps and from the MA office, with additional
support from the UK if necessary. Getting the upgrade with your service
contract renewal should be straightforward.

Regards,

Elaine

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited.
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-----Original Message-----
X-from: alex.titkov-at-millenniumchem.com
[mailto:alex.titkov-at-millenniumchem.com]
Sent: Tuesday, November 15, 2005 12:56 AM
To: Elaine F. Schumacher

Hi,

Can anyone please tell me what the latest issue of The Microanalysis
Suite
from Oxford Instruments is currently available?
What issue are you using? We are on issue 13. Our service contract
entitles
us for free software upgrades, but we just can not get the information
from
the local Oxford representatives.

Many thanks,
Alexander Titkov

Millennium Inorganic Chemicals Ltd
A Lyondell Company






Lyondell Chemical Company

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Lyondell Chemical Company

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From: monica.iliescu-at-polymtl.ca
Date: Wed, 16 Nov 2005 06:59:12 -0600
Subject: [Microscopy] viaWWW: ESEM meetings?

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Email: monica.iliescu-at-polymtl.ca
Name: Monica ILIESCU

Organization: Ecole Polytechnique

Title-Subject: [Filtered] MListserver:

Question: Dear colleagues,
I would like to ask if someone of you know conferences involving (environmental) electron microscopy and biomaterials or related biological materials taking place in 2006.
Thank you very much.

---------------------------------------------------------------------------

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From: MSHERWOOD-at-PARTNERS.ORG
Date: Wed, 16 Nov 2005 08:23:45 -0600
Subject: [Microscopy] re: New England Society for Microscopy (NESM) Fall Symposium

Contents Retrieved from Microscopy Listserver Archives
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The Fall Symposium of the New England Society for Microscopy will once again be
held at Gordon College in Wenham, MA. It will be held on Thursday, December 1st
from Noon - 8:30pm. The program highlights talks on various microscopy
techniques. For more detailed information, including registration, the speakers
and talks, please go to NESM's website: http://nesm.cims.harvard.edu and click
on "current newsletter".

The deadline for registration (including dinner) is Monday, November 28th.
Please contact Paul Bain, Treasurer at Paul_Bain-at-hms.harvard.edu.

We look forward to seeing you at the meeting!

Peggy Sherwood
Corresponding Secretary, NESM







Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
msherwood-at-partners.org


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From: klk-at-biotech.ufl.edu
Date: Wed, 16 Nov 2005 14:23:28 -0600
Subject: [Microscopy] starch in sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am looking for a technique that will help identify starch in
osmicated/Embed-Araldite embedded sections. One suggestion has been to
use amylase digestion or amylase/gold. Any advice or other suggestions
would be greatly appreciated.

--
Karen L. Kelley
ICBR Electron Microscopy Manager
University of Florida
ICBR Electron Microscopy Core Lab
Bartram Hall Room 214
Box 118525 Gainesville Florida
Lab: 352-392-1184 fax: 352-846-0251
Email: klk-at-biotech.ufl.edu
Southeastern Microscopy Society Treasurer
http://www.biotech.ufl.edu/EM/



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From: baskin-at-bio.umass.edu
Date: Wed, 16 Nov 2005 16:56:18 -0600
Subject: [Microscopy] Re: starch in sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Karen,
Starch grains are beautifully birefringent so they show up a
treat in polarized light microscopy. If you can use semi thin
sections to find/demonstrate them, you would be home free. In an
ultra-thin section, the polarized light signal would be low, so you
might need a sensitive instrument to detect them but I think still
quite possible. Of course this is at the light level, not TEM. Hope
it helps.

Tobias Baskin

}
}
} I am looking for a technique that will help identify starch in
} osmicated/Embed-Araldite embedded sections. One suggestion has been to
} use amylase digestion or amylase/gold. Any advice or other suggestions
} would be greatly appreciated.
}
} --
} Karen L. Kelley
} ICBR Electron Microscopy Manager
} University of Florida
} ICBR Electron Microscopy Core Lab
} Bartram Hall Room 214
} Box 118525 Gainesville Florida
} Lab: 352-392-1184 fax: 352-846-0251
} Email: klk-at-biotech.ufl.edu
} Southeastern Microscopy Society Treasurer
} http://www.biotech.ufl.edu/EM/
}
}
}
} =======


--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
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From: barbara.miner-at-intel.com
Date: Wed, 16 Nov 2005 21:41:34 -0600
Subject: [Microscopy] viaWWW: Job Opening at INTEL , TEM sample prep technician

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Email: barbara.miner-at-intel.com
Name: Miner, Barbara

Organization: INTEL

Title-Subject: [Filtered] Experienced TEM sample prep technician

Question: Job Title: experienced TEM sample prep technician
Location: Hillsboro, OR
Start date: December 2005 or January 2006
Requisition will close at the end of November

Intel Corporationís Materials Analysis Lab, supporting development of next generation micro-processors, has immediate openings for experienced Transmission Electron Microscopy sample prep technicians in Hillsboro, Oregon. ÝÝSuccessful applicants must have experience working with patterned Si wafers, operating dual-beam FIBs, and applying precision mechanical polish. Candidate must have excellent attention to detail and the ability to multi-task. Good communication, problem-solving skills, and cooperative team work are mandatory. The work shift includes one weekend day. Relocation assistance is provided.

As the world's largest chip manufacturer, Intel strives to make every facet of semiconductor manufacturing state-of-the-art -- from semiconductor process development and manufacturing, through yield improvement to final test and optimization, and lastly packaging. Employees in the Technology and Manufacturing group are part of a worldwide network of manufacturing and assembly/test facilities.

Intel only accepts resumes/CV's submitted to our web site. To enable us to process your application, please submit your resume/CV to www.intel.com/jobs. ÝPlease, also send mail to {mailto:Barbara.miner-at-intel.com} Barbara.miner-at-intel.com indicating your intent to apply. ÝPlease note the job title and location to complete your search and application. Intel hires qualified candidates who are authorized to work in the U.S.-- that is, authorized to work without restriction as to a particular employer. This includes U.S. citizens or nationals, U.S. legal permanent residents, temporary residents granted legalization under the Immigration Reform and Control Act of 1986, asylees, and refugees. For foreign nationals who do not fall in one of the above categories, we limit our hiring of persons requiring visa sponsorship or individuals currently on a non-immigrant visa (e.g., H-1, J-1, L-1, F-1, B-1, TN) to candidates at the MS and PhD levels (or those who have equivalent work experience) who are applying for positions for which there is a demonstrated shortage of qualified U.S. candidates. At Intel, we are committed to equal employment opportunity. We respect, value and welcome diversity in our workforce, as well as in our customers, our suppliers and the global marketplace. Intel also values being a great place to work and strives to maintain a safe and drug-free workplace. Accordingly, Intel conditions all offers of employment on satisfactory completion of a drug screen (where allowed) and a background check. Intel does not accept resumes from headhunters or suppliers that have not signed a formal fee agreement. Our supplier base is limited to specific hiring needs. Therefore, any resume received from an unapproved supplier will be considered unsolicited, and Intel will not be obligated to pay a referral fee.


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From: mike.reedy-at-cellbio.duke.edu
Date: Thu, 17 Nov 2005 01:27:41 -0600
Subject: [Microscopy] Re: SEM imaging of bacteria encapsulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Karl,
Just to offer a heretically simplifed option for quick-and-dirty (but
often unexpectedly clean) speedy results:
I agree with Muss that tannic acid is great, the surest simple
fixative, and with Webster that freezing is a promising approach.

But I suggest that for fixing extracellular matrix, and intracellular
structures of detergent (TX-100) pre-permeabilized cells, you can do
quite well without OsO4; just use freshly dissolved 0.2% TA in your
physiol extracellular or intracellular buffer at pH 6.8-7.0 (absent
any components that precipitate in TA) for 30 min, then wash out all
unbound TA in 5 buffer changes, and follow with 1% UrAc in DI water,
no pH adjustment needed, then do acetone or EtOH dehydration as fast
as you like. If you wish, you can interpose 0.5-2.0 hr in 1%
glutaraldehyde after the TA and before the UrAc, but i usually use
straight TAURAC as a preferred binary fix; is certainly less toxic
and just as fixing as a similar TAOS procedure.
(M. K. Reedy, C. Lucaveche, D. Popp, Biophysical Journal 59,
579a (1991)).
Worth one shot for comparison is using a TA-glut mixture as a trial
primary fix before UrAc, but I find glut sometimes tends to rush in
and perturb orderly lattice arrays that TA alone fixes more slowly
and beautifully. TA primary fix MUST be stabilized by UrAc or OsO4
secondary fix before dehydration.

I've used the same TAURAC sequence in cryo-acetone for
freeze-substitution almost exclusively for 15 years. that's what
suggests to me its possible value for polysaccharides. It is able to
fix and stabilize (or perhaps it just permits PHYSICAL in-situ
stabilizing of) the threadlike molecules of 500,000 MW dextran we
sometimes use at 3-5% as an osmotic squeezer for the myofilament
lattice of glycerinated muscle fibers. We can see them in thin
sections, excluded to the space between myofibrils. However, the
aqueous TAURAC fix does NOT preserve /retain the free dextran
molecules, so I guess the quick-freezing and/or acetone is needed for
that.

So I guess maybe using an initial non-cryo "fix" of acetone alone,
followed by TAURAC in acetone and comparing with acetone-TAURAC
sequence and with an acetone-URAC-TA sequence might be worth trying.
Note Craig lab's findings that UrAc alone can be a fabulous fixative.
(F. Q. Zhao, R. Craig, Journal of Structural Biology 141, 43
(Jan, 2003).

And if you want to try a totally safer alternative than propane for
the plunge-freezing that Paul Webster suggests, LN2 plunging has a
bad rap that is undeserved. If you plunge rapidly through 10-12
inches of LN2, you leave behind all the insulating bubbles
responsible for the Leidenfrost effect and get convective cooling at
least as rapid or more-so than propane provides. See what protein
crystallographers from Hope's lab have to say about how reliable this
can be!
(L. J. Walker, P. O. Moreno, H. Hope, Journal of Applied
Crystallography 31, 954 (1998); S. Parkin, H. Hope, Journal of
Applied Crystallography 31, 945 (1998)).

Best of luck!
--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html

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From: justgetmeonhere-at-netscape.net
Date: Thu, 17 Nov 2005 07:13:46 -0600
Subject: [Microscopy] viaWWW: question about BSE detectors

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Email: justgetmeonhere-at-netscape.net
Name: Matthew McCaskill

Organization: none

Title-Subject: [Filtered] question about BSE detectors

Question: Hello all,

I am enrolled in an SEM class, and for this class I have been asked to do a paper on backscattered electron (BSE) detectors. Specifically, I have to briefly state what is state-of-the-art now, and then discuss research or improvements that are occurring right now. Then I am to predict and forecast the future of the technology; will there be more developments, or have BSE detectors fully matured technologically?

The point of all this post, however, is that I'm having trouble finding literature on SEM in general, let alone BSE detectors. Utilizing my school's library and the internet, I have found at least four articles already, but I need at least four more. Can anybody recommend me some journal articles or papers that deal with my topic? Preferably, I'd like for them to be available as FREE electronic copies (I found a few promising articles online, but unfortunately I have to pay to view, which is disadvantageous to poor college students such as myself), but if I can get ahold of the actual hard copies at my school's library then that should also be enough.

Thanks to all who can help me in this regard, and thanks to all of you for your time.

---------------------------------------------------------------------------

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From: L.Tilley-at-latrobe.edu.au
Date: Thu, 17 Nov 2005 07:21:37 -0600
Subject: [Microscopy] viaWWW: Post Doc Position Open - ultrastructural biology

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Email: L.Tilley-at-latrobe.edu.au
Name: Leann Tilley

Organization: La Trobe University

Title-Subject: [Filtered] Post Doc Position Open - ultrastructural biology

Question: A postdoctoral position (Level A/B) is available to work on ultrastructural biology in a new ARC Centre of Excellence for Coherent X-ray Science. ( {http://www.coecxs.org/http://www.coecxs.org/). This position is funded by the Australian Research Council as part of the Centre of Excellence for Coherent X-ray Science for research into the use of novel X-ray diffraction techniques to study the cellular architecture of malaria parasite-infected erythrocytes and other biological samples. The project involves adapting sample preparation methods developed for electron microscopy to generate samples suitable for X-ray microscopy and other X-ray imaging techniques. The successful applicant will work with colleagues from the Departments of Biochemistry and Physics, at La Trobe University, and other Centre members to image the ultrastructure of malaria parasites and other samples. The applicant will also prepare samples for cyro-electron microscopy and single particle image reconstruction techniques. Molecular biological manipulation of cells and proteins will also be used in enhancing sample preparation.

Further information, contact:
Prof. Leann Tilley. Department of Biochemistry, La Trobe University.
Tel: 9479 1375.
Email: {mailto:L.Tilley-at-latrobe.edu.auL.Tilley-at-latrobe.edu.au


Prof Leann Tilley
Phone: 61-3-94791375 Department of Biochemistry
Fax: 61-3-94792467 Plenty Rd, La Trobe University
Email: L.Tilley-at-latrobe.edu.au Melbourne, 3086 Australia http://www.latrobe.edu.au/biochemistry/labs/tilley/research.html

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From: eschumacher-at-mccrone.com
Date: Thu, 17 Nov 2005 08:40:07 -0600
Subject: [Microscopy] Second Announcement: Student Poster Competition - MMMS Meeting March 24, 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

SECOND ANNOUNCEMENT

STUDENT POSTER COMPETITION ANNOUNCEMENT

To be held in conjunction with the March 24, 2006 meeting of the Midwest

Microscopy and Microanalysis Society

Affiliate of the Microscopy Society of America and the Microbeam
Analysis
Society of America

ABSTRACT DEADLINE: Abstracts must be received in electronic format by
5PM, Friday, December 16, 2005.

The first quarter meeting of the Midwest Microscopy and Microanalysis
Society will be held on Friday, March 24, 2006, in the Pancoe Life
Sciences Building, Northwestern University, Evanston, Illinois. A
student poster competition open to undergraduate and graduate students
will be held in
conjunction with the meeting. Posters should illustrate utilization of
microscopy for either biological or materials science study. Prizes
will be awarded as follows:

$300 for 1st place
$200 for 2nd place
$100 for 3rd place

Abstracts must be received in electronic format by 5PM on Friday,
December 16, 2005. To be eligible for a prize, you must be first author
on the poster, and you must be present at the meeting. You are
encouraged to submit your entry as early as possible, as space may be
limited. Abstracts from last year's competition, and an example of the
judging worksheet can be found on the MMMS website:


http://northwestern.edu/bioimaging/MMMS%20Website/index.htm


Further details will be provided upon acceptance of your submission.

Please send the following information to the email address below, and
attach your abstract as a Word document:

Name Phone number
Affiliation Email address
Mailing address

Send to:

Elaine Schumacher
MMMS Materials Science Director
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539
Tel: 630-887-7100 Fax: 630-887-7417
Email: eschumacher-at-mccrone.com


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From: Jane.LaGoy-at-bodycote.com
Date: Thu, 17 Nov 2005 09:25:35 -0600
Subject: [Microscopy] viaWWW: question about BSE detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Matthew - I would contact the SEM manufacturer, JEOL (978-535-5900), and the
EDS manufacturers EDAX (201-529-4880) and PGT (609-924-7310), or check their
websites; I have seen technical articles regarding this authored by their
employees, and there are probably other SEM/EDS companies with helpful
literature as well.

Jane L. LaGoy
Lab Manager/R&D Engineer
978-470-1620 x450
jane.lagoy-at-bodycote.com

BODYCOTE NORTH AMERICA · 155 RIVER STREET · ANDOVER · MASSACHUSETTS ·
01810
TEL: (978) 470-1620 · FAX: (978) 475-2951 · ONLINE: www.bodycote.com




-----Original Message-----
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Email: justgetmeonhere-at-netscape.net
Name: Matthew McCaskill

Organization: none

Title-Subject: [Filtered] question about BSE detectors

Question: Hello all,

I am enrolled in an SEM class, and for this class I have been asked to do a
paper on backscattered electron (BSE) detectors. Specifically, I have to
briefly state what is state-of-the-art now, and then discuss research or
improvements that are occurring right now. Then I am to predict and forecast
the future of the technology; will there be more developments, or have BSE
detectors fully matured technologically?

The point of all this post, however, is that I'm having trouble finding
literature on SEM in general, let alone BSE detectors. Utilizing my school's
library and the internet, I have found at least four articles already, but I
need at least four more. Can anybody recommend me some journal articles or
papers that deal with my topic? Preferably, I'd like for them to be
available as FREE electronic copies (I found a few promising articles
online, but unfortunately I have to pay to view, which is disadvantageous to
poor college students such as myself), but if I can get ahold of the actual
hard copies at my school's library then that should also be enough.

Thanks to all who can help me in this regard, and thanks to all of you for
your time.

---------------------------------------------------------------------------

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From: apnewell-at-ncsu.edu
Date: Fri, 18 Nov 2005 07:23:37 -0600
Subject: [Microscopy] viaWWW: low voltage STEM

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Email: apnewell-at-ncsu.edu
Name: AndyNewell

Organization: AREMC / NC State

Title-Subject: [Filtered] low voltage STEM

Question: I am looking for literature on current developments of SEM-based STEM (low kV) and/or names of predominant researchers that have benefitted recently by using STEM in an SEM for thin sample characterization and microanalysis. Information on other improvements such as aberration corrections that have helped low-voltage STEM or speculation on the next step in low energy STEM development is also welcome.

Many of us graduate students here at NCSU are having difficulty finding publications on development of the technology due to the proprietary nature of such developments, but I am sure there is some fine work going on in the scientififc community. Can anyone help me locate the works of some SEM-based STEM heavy-hitters?

Any assistance available is greatly appreciated.

Many thanks,
Andy Newell
Materials Science & Engineering
NC State University


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From: rbeavers-at-mail.smu.edu
Date: Fri, 18 Nov 2005 11:51:30 -0600
Subject: [Microscopy] Imaging bug fossils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Group

l have been trying to look at some bug fossils in rock samples with SEM. In hand specimen these bugs stand out as dark areas against light tan rock material. In the SEM using backscatter they tend to fade into the background which seems to say there is not much organic material left. SEM secondary electron images do not yeild much information either.

Would like to hear any ideas on other techniques people have use to image such samples.

Thanks

Roy Beavers

Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



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From: avklaus-at-amnh.org
Date: Fri, 18 Nov 2005 12:42:24 -0600
Subject: [Microscopy] Imaging bug fossils

Contents Retrieved from Microscopy Listserver Archives
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Hi Roy,

Depending on the composition of the bug fossils, you might have some luck
trying high-resolution CT scanning (microCT). I know of some microCT
scanning successes of mantis fossils with high iron content compared to the
surrounding rock matrix. Although if you are not seeing much average atomic
number difference by BSE imaging, maybe CT would also not work well.

If you need more info on microCT of bugs, please don't hesitate to contact
me offline.

Best regards,

Angela


Angela V. Klaus, Ph.D.
Director, Microscopy and Imaging Facility
American Museum of Natural History
Central Park West and 79th Street
New York, NY 10024 USA

Tel: 212-769-5977
Email: avklaus-at-amnh.org

-----Original Message-----
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Group

l have been trying to look at some bug fossils in rock samples with SEM. In
hand specimen these bugs stand out as dark areas against light tan rock
material. In the SEM using backscatter they tend to fade into the background
which seems to say there is not much organic material left. SEM secondary
electron images do not yeild much information either.

Would like to hear any ideas on other techniques people have use to image
such samples.

Thanks

Roy Beavers

Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



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From: oshel1pe-at-cmich.edu
Date: Fri, 18 Nov 2005 14:15:01 -0600
Subject: [Microscopy] RE: Imaging bug fossils

Contents Retrieved from Microscopy Listserver Archives
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Depending on the BSE detector you are using, you might not see much
contrast. We had a 4-quadrant solid-state detector mounted coaxially
with the beam. It does not show much topography when all four quadrants
are summed together. That mode does show the best composition contrast,
but I don't know how much compositional contrast you should expect for
fossils in rock.

Are the fossilizing minerals the same as the host rock? If not, you
should be able to get elemental contrast via an x-ray map image. BTW,
you may need to experiment with the beam voltage depending on the
thickness of your "bugs". A 20kV beam could be blasting all the way
through them.

Still, I would remain hopeful. I know that optical contrast does not
always translate into contrast in the EM, but often there is some way to
tease out contrast in some imaging mode or another.

Warren Straszheim

-----Original Message-----
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Sent: Friday, November 18, 2005 11:53 AM
To: wesaia-at-iastate.edu

Roy,

Two thoughts come to mind. First, if you have access to a low-kV SEM, I'd try imaging at 1 or 1.5 kV with secondaries. You'd more likely differentiate the fossil from the matrix this way. The other thought is: are this organic films? phosphatized? ... ? Meaning, have you tried etching the rocks with weak acetic acid? This often works to bring out fossils (or remove them from the matrix entirely).
It might also be the worst possible thing to do, and should be tried first on a fossil that doesn't matter.

Phil

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

Group

l have been trying to look at some bug fossils in rock samples with SEM. In hand specimen these bugs stand out as dark areas against light tan rock material. In the SEM using backscatter they tend to fade into the background which seems to say there is not much organic material left. SEM secondary electron images do not yeild much information either.

Would like to hear any ideas on other techniques people have use to image such samples.

Thanks

Roy Beavers

Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



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From: gary-at-gaugler.com
Date: Fri, 18 Nov 2005 14:36:02 -0600
Subject: [Microscopy] Re: Imaging bug fossils

Contents Retrieved from Microscopy Listserver Archives
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What exactly are you trying to accomplish? If you want
morphology, low KV SE should do it. BSE is just going
to show difference in Z. Since the bug is or was organic,
it ought not show much with BSE.

If you want to do morphology and save the specimen, perhaps
doing a replica would work. Use replica resin and then sputter
coat it and then SEM it. Invert the image and you should
have excellent morphology results.

Another idea is CL.

gary g.


At 09:53 AM 11/18/2005, you wrote:



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From: DRK-at-SHCC.org
Date: Fri, 18 Nov 2005 19:15:55 -0600
Subject: [Microscopy] TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
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Hi,

We've struggled all we can to to keep our Ilford photo processor
working. It's time we entered the digital age. Can anyone suggest a
TEM negative scanner? Ideally it would take a stack of negatives and
feed them into the scanner and produce individual files, so if anyone
can suggest a model that does this I'd be ecstatic. Otherwise, if you
have a scanner that will automatically scan 12 or more negatives,
producing individual files of each, please share your experience.

Many thanks,

doug

Douglas R. Keene
Assistant Investigator
Micro-Imaging Center
Research Department
Shriners Hospital for Children
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97239-3009
voice: 503-221-3434


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From: jmcss-at-aol.com
Date: Sat, 19 Nov 2005 07:57:49 -0600
Subject: [Microscopy] AskAMicroscopist: Middle School - Leaf Stomata Question

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This Question was submitted to Ask-A-Microscopist by (jmcss-at-aol.com)
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Email: jmcss-at-aol.com
Name: Mary Hamelin

Organization: Country Day School

Education: 6-8th Grade Middle School

Location: Groton, MA

Title: plants

Question: What is the best way to help the students visualize stomata using a microscope? I have tried clear nail polish to make a relief of the leaf's exterior but didn't have much luck. Is there a way to make good quality, thin sections of plant material with little equipment? I'd like to help the students discover different types of plant cells. Also, what staining, if any would you recommend?

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From: sarj0007-at-unf.edu
Date: Sat, 19 Nov 2005 08:02:13 -0600
Subject: [Microscopy] viaWWW: Joel 35-CF "Smoking"

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Email: sarj0007-at-unf.edu
Name: Jason Saredy

Organization: University of North Florida

Title-Subject: [Filtered] Joel 35-CF

Question: Hi. We are trying to get an old Joel-35CF SEM up and running from a long period of not being used. When turned on there is smoke coming out of the inside of the voltage splitter where, according to the schematics, the transformers are. The smoke doesnít start to come out until about 30-40 seconds after being turned on and its pulling at least 5 amps from a circuit that should only be pulling 3 amps. Anyone knows whether this is an easy to replace part or should we replace the whole power supply. We are doing this out of pocket so prefer to not purchase a new one directly from Joel. If so does anyone have some parts lying around? Or a good website for finding this sort of information? Thanks

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From: jbradley-at-igpp.ucllnl.org
Date: Sat, 19 Nov 2005 08:03:32 -0600
Subject: [Microscopy] viaWWW: A Noran TN5500 program

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Email: jbradley-at-igpp.ucllnl.org
Name: John Bradley

Organization: Lawrence Livermore Laboratory

Title-Subject: [Filtered] SMTF - A Noran TN5500 program

Question: I am looking for a copy of the Noran program "SMTF" (Standardless Metallurgical Thin Film).

I have several hundred TEM/EDX spectra archived on 5.25 inch floppy discs. I also have access to a TN5500 with a 5.25 inch floppy disc drive. I need is a copy of SMTF on a 5.25 inch floppy disc.

I would also very much like to know how these files can be exported from the TN5500.

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From: watson-at-wi.mit.edu
Date: Sat, 19 Nov 2005 08:04:23 -0600
Subject: [Microscopy] viaWWW: Iodine 125/silver enhancement

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Email: watson-at-wi.mit.edu
Name: Nicki Watson

Organization: whitehead Institute

Title-Subject: [Filtered] Iodine 125/silver enhancement

Question: Hi
I am looking for protocols for immunolabeling cultured cells using Iodine 125/silver enhancement techniques.
I think this is a rather old fashion method, but I have a professor interested in it for a specific project. We would also be interested in
the opinions of you experts as to the functionality of this technique.Do you prefer gold, or peroxidase, quantum dots ? Does the iodine 125 have any special features that make it a great choice? Any input would be great!
thanks for your input
Nicki Watson

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From: baskin-at-bio.umass.edu
Date: Sat, 19 Nov 2005 10:41:33 -0600
Subject: [Microscopy] Re: AskAMicroscopist: Middle School - Leaf Stomata

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mary,
I am not an expert in this area but I can suggest a couple of
things to try.

First, check out Project Micro, this has a ton of useful information
about microscopes in education, for all levels.
http://microscopy.org/ProjectMICRO/

Second, don't forget to see what you can see with a good hand lens,
for example a jewler's loupe. This shows a great deal (though not
usually cells) and is a great intermediate between the eye and the
microscope. Lots of leaves (flowers, etc) make elaborate hairs and
things, which look great even with modest magnification.

Third, remember that in many (most?) plants, stomata are on the
abaxial side of the leaf (ie, towards the ground).

Fouth. A trick popular with stomatal researchers is to make an
epidermal peel. You take a tweezers and grab near the midvein and
carefully pull toward the leaf margin. You can often remove a flap of
epidermis. Put that in some water on a slide under a coverslip and
you should be able to see stomata even in brighfield. Supposedly
Vicia faba (bean) is easy to peel. But note that for your purpose,
even a small (few mm square) area should suffice. It might be fun for
your class to try some different plants and see which peel better?

Fifth. If you do get good at peeling, you can even try some things to
open or close the stomata. Add some CaCl2 to the water where the
peels are, or sodium bicarbonate. See what happens. Manipulate the
light environment of the plants before you make peels (keep some
plants in the dark, others in bright light). This could be beyond
what you have in mind, but I thought I'd mention it just in case.

Have fun

Hope this helps,
Tobias Baskin

}
}
} Email: jmcss-at-aol.com
} Name: Mary Hamelin
}
} Organization: Country Day School
}
} Education: 6-8th Grade Middle School
}
} Location: Groton, MA
}
} Title: plants
}
} Question: What is the best way to help the students visualize
} stomata using a microscope? I have tried clear nail polish to make a
} relief of the leaf's exterior but didn't have much luck. Is there a
} way to make good quality, thin sections of plant material with
} little equipment? I'd like to help the students discover different
} types of plant cells. Also, what staining, if any would you
} recommend?
}

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \
University of Massachusetts
/ / / \ \ \
Amherst, MA, 01003
/ / ___ / \ \__/ \ ____ Voice: 413 - 545 - 1533
Fax: 413 - 545 - 3243
http://www.bio.umass.edu/biology/baskin/

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From: cbalane-at-wesleyan.edu
Date: Sat, 19 Nov 2005 13:35:26 -0600
Subject: [Microscopy] gold conjugation with fluorescent BSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,
I am trying to conjugate my colloidal gold particles (0.8 nm and 6 nm
particle sizes) with fluoresceinated BSA. I have tried to perform
extensive literature search for detailed protocols but most of what I
found were either for specific proteins (like IgG or Protein A) or general
recipes that don't really say much about what I should do.

I know that we have experts out there who have done such procedures (or
others that may be very similar) in the past; any advice/information would
be greatly appreciated.

Have a happy thanksgiving!

Sincerely,
Carlo

---

Carlo Franco Bolivar Balane

Box 4058, 222 Church Street, or Wolfe Laboratory
Wesleyan University Station Rm. 157, HA Laboratories
Middletown, CT, 06459-4058 Wesleyan University
phone: 1.860.759.2830 phone: 1.860.685.3275


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From: mike.reedy-at-cellbio.duke.edu
Date: Sat, 19 Nov 2005 17:15:13 -0600
Subject: [Microscopy] Re: TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Doug,
The $350-500 Epson and Canon flatbeds seem hard to beat for price and
resolution, but don't have film autofeeders or multi-holders for 3.25
x 4" negatives; you must fit one negative at a time into the 4"x5"
holder. I'm not sure jamproof autofeeders exist.

600 dpi scans will serve most workprint needs (600 scans 29 sec or
less with new EPSON 4990 PRO, says Amazon). Keep the film! for
selected re-scan when needing greater enlargement. My betters advise
that it is better (more dynamic range/) to scan as a transparency and
invert rather than as a negative. Make excellent workprints fast on
standard paper on an HP 4100 LaserJet set for 150 lpi. (exceeds
printer spec of 1200 dpi, I know, but it works).

For general info look at http://scantips.com/ .

For additional general info look over many comments and viewpoints at
http://www.macintouch.com/scanners05.html (also parts 4 amd 6)
Note Steve Pucci's comments on batch-feed film scanners under Aug
25, 2005 entries.
Check out his preferred Canon 9950 scanner for its Customer Reviews
comments at Amazon.com; then see also the Epson Perfection 4870 PRO
Customer Reviews. The newest Epson, 4990 PRO, has no Amazon reviews
yet, but see this and other scanners, including Microtek, reviewed at
CNET:
http://reviews.cnet.com/Epson_Perfection_4990_Pro/4514-3136_7-31320383.html
(latter site also has reviews and customer comments for the other top
scanners).

-mike reedy


At 7:20 PM -0600 11/18/05, DRK-at-SHCC.org wrote:
} ----------------------------------------------------------------------------
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--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
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From: rpowell-at-nanoprobes.com
Date: Sun, 20 Nov 2005 09:10:32 -0600
Subject: [Microscopy] viaWWW: gold conjugation with fluorescent BSA

Contents Retrieved from Microscopy Listserver Archives
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Email: rpowell-at-nanoprobes.com
Name: Richard D. Powell

Organization: Nanoprobes, Incorporated

Title-Subject: [Filtered] Re: [Microscopy] gold conjugation with fluorescent BSA

Question: Hello Carlo:

Most colloidal gold conjugation protocols are for antibodies or other targeting agents. BSA is used as a stabilizer in these reactions, but because conjugation of the targeting agent takes priority, the reaction conditions are usually optimized for conjugating to this rather than the BSA. However, you don't need to make many changes - the procedure is similar for most colloidal gold conjugations.

Optimum colloidal gold conjugation is usually done at a pH at or just above the pI of the protein you are conjugating. From a quick search, the pI of BSA is about 4.7, so a pH of about 5 to 5.2 would be good for conjugation. Usually you would dialyze the protein into a dilute buffer at this pH or deionized water; use a buffer that does not flocculate colloidal gold (for example 0.002 M citrate, pH 5). Adjust the pH of the colloidal gold sol to the same pH using dilute H3PO4; then add the minimum stabilizing amount of fluorescein-BSA to the gold (you can determine this from titration; a procedure is given in the link below), stir for 2 minutes, then add a further 10% of BSA and 0.1% carbowax (a special form of 20,000 MW polyethylene glycol), stir 10 - 15 minutes more.

A good procedure for antibodies is given in this link. To adapt for albumin, all you need to do is to change the pH for the titration and conjugation to one more suited to BSA (adjust pH with H3PO4 if you need to lower rather than raise it):

http://www.researchd.com/gold/gold8.htm

All glassware must be scrupulously clean. Glass and plastic containers and stirrers should be cleaned in aqua regia, thoroughly washed in deionized water, and siliconized.

Once you have prepared the conjugate, you should store at a pH several units away from the PI, as this helps stabilize the conjugate. Spin down and resuspend in 0.02 M Tris-HCl, pH 8.2 (the usual buffer for storing colloidal gold conjugates).

Before doing this, though, you should be aware that fluorescence will likely be completely quenched if you conjugate fluorescein-streptavidin to 6 nm gold - gold is a very efficient absorber for resonance energy transfer, and other mechanimsms may also be present that will quench the fluorescence even more than energy transfer alone. We have explained this, and Albrecht and co-workers have observed it in practice when making combined fluorescent and 6 nm gold-labeled antibodies:

(1) Powell, R. D.; Halsey, C. M. R., and Hainfeld, J. F.: Combined fluorescent and gold immunoprobes: Reagents and methods for correlative light and electron microscopy. Microsc. Res. Tech., 1998, 42, 2.

(2) Kandela, I. K.; Meyer, D. A.; Oshel, P. E.; Rosa-Molinar, E., and Albrecht, R. M.: Fluorescence Quenching by Colloidal Heavy Metals: Implications for Correlative Fluorescence and Electron Microscopy Studies. Microsc. Microanal., 9, (Suppl. 2: Proceedings); Piston, D.; Bruley, J.; Anderson, I. M.; Kotula, P.; Solorzano, G.; Lockley, A., and McKernan, S. (Eds.); Cambridge University Press, New York, NY, 2003, 1194CD.

Hope this is helpful,

Rick Powell

*****************************************************************************************
Richard D. Powell
www.nanoprobes.com
NANOPROBES, Incorporated
*****************************************************************************************

Hi everyone,
I am trying to conjugate my colloidal gold particles (0.8 nm and 6 nm
particle sizes) with fluoresceinated BSA. I have tried to perform
extensive literature search for detailed protocols but most of what I
found were either for specific proteins (like IgG or Protein A) or general
recipes that don't really say much about what I should do.

I know that we have experts out there who have done such procedures (or
others that may be very similar) in the past; any advice/information would
be greatly appreciated.

Have a happy thanksgiving!

Sincerely,
Carlo

---

Carlo Franco Bolivar Balane

Box 4058, 222 Church Street, or Wolfe Laboratory
Wesleyan University Station Rm. 157, HA Laboratories
Middletown, CT, 06459-4058 Wesleyan University
phone: 1.860.759.2830 phone: 1.860.685.3275


---------------------------------------------------------------------------

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From: rpowell-at-nanoprobes.com
Date: Sun, 20 Nov 2005 09:28:51 -0600
Subject: [Microscopy] viaWWW: gold conjugation with fluorescent BSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
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Email: rpowell-at-nanoprobes.com
Name: Richard D. Powell

Organization: Nanoprobes, Incorporated

Title-Subject: [Filtered] Re: [Microscopy] gold conjugation with fluorescent BSA

Question: Hello Carlo:

Most colloidal gold conjugation protocols are for antibodies or other targeting agents. BSA is used as a stabilizer in these reactions, but because conjugation of the targeting agent takes priority, the reaction conditions are usually optimized for conjugating to this rather than the BSA. However, you don't need to make many changes - the procedure is similar for most colloidal gold conjugations.

Optimum colloidal gold conjugation is usually done at a pH at or just above the pI of the protein you are conjugating. From a quick search, the pI of BSA is about 4.7, so a pH of about 5 to 5.2 would be good for conjugation. Usually you would dialyze the protein into a dilute buffer at this pH or deionized water; use a buffer that does not flocculate colloidal gold (for example 0.002 M citrate, pH 5). Adjust the pH of the colloidal gold sol to the same pH using dilute H3PO4; then add the minimum stabilizing amount of fluorescein-BSA to the gold (you can determine this from titration; a procedure is given in the link below), stir for 2 minutes, then add a further 10% of BSA and 0.1% carbowax (a special form of 20,000 MW polyethylene glycol), stir 10 - 15 minutes more.

A good procedure for antibodies is given in this link. To adapt for albumin, all you need to do is to change the pH for the titration and conjugation to one more suited to BSA (adjust pH with H3PO4 if you need to lower rather than raise it):

http://www.researchd.com/gold/gold8.htm

All glassware must be scrupulously clean. Glass and plastic containers and stirrers should be cleaned in aqua regia, thoroughly washed in deionized water, and siliconized.

Once you have prepared the conjugate, you should store at a pH several units away from the PI, as this helps stabilize the conjugate. Spin down and resuspend in 0.02 M Tris-HCl, pH 8.2 (the usual buffer for storing colloidal gold conjugates).

Before doing this, though, you should be aware that fluorescence will likely be completely quenched if you conjugate fluorescein-streptavidin to 6 nm gold - gold is a very efficient absorber for resonance energy transfer, and other mechanimsms may also be present that will quench the fluorescence even more than energy transfer alone. We have explained this, and Albrecht and co-workers have observed it in practice when making combined fluorescent and 6 nm gold-labeled antibodies:

(1) Powell, R. D.; Halsey, C. M. R., and Hainfeld, J. F.: Combined fluorescent and gold immunoprobes: Reagents and methods for correlative light and electron microscopy. Microsc. Res. Tech., 1998, 42, 2.

(2) Kandela, I. K.; Meyer, D. A.; Oshel, P. E.; Rosa-Molinar, E., and Albrecht, R. M.: Fluorescence Quenching by Colloidal Heavy Metals: Implications for Correlative Fluorescence and Electron Microscopy Studies. Microsc. Microanal., 9, (Suppl. 2: Proceedings); Piston, D.; Bruley, J.; Anderson, I. M.; Kotula, P.; Solorzano, G.; Lockley, A., and McKernan, S. (Eds.); Cambridge University Press, New York, NY, 2003, 1194CD.

Hope this is helpful,

Rick Powell

*****************************************************************************************
Richard D. Powell
www.nanoprobes.com
NANOPROBES, Incorporated
*****************************************************************************************

Hi everyone,
I am trying to conjugate my colloidal gold particles (0.8 nm and 6 nm
particle sizes) with fluoresceinated BSA. I have tried to perform
extensive literature search for detailed protocols but most of what I
found were either for specific proteins (like IgG or Protein A) or general
recipes that don't really say much about what I should do.

I know that we have experts out there who have done such procedures (or
others that may be very similar) in the past; any advice/information would
be greatly appreciated.

Have a happy thanksgiving!

Sincerely,
Carlo

---

Carlo Franco Bolivar Balane

Box 4058, 222 Church Street, or Wolfe Laboratory
Wesleyan University Station Rm. 157, HA Laboratories
Middletown, CT, 06459-4058 Wesleyan University
phone: 1.860.759.2830 phone: 1.860.685.3275


---------------------------------------------------------------------------

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From: heckman-at-bgnet.bgsu.edu
Date: Sun, 20 Nov 2005 14:12:38 -0600
Subject: [Microscopy] Re: gold conjugation with fluorescent BSA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Carlo-

Gold particles (and in fact, most clean surfaces of a noble metal,
will bind to any type of protein. The binding is apparently not
covalent but it is very tight. If you follow a procedure for binding
IgG or Protein A, but what you supply is only BSA, the BSA will bind.
BSA does not adhere to surfaces as tightly as many other proteins.
There is a lot of literature on the adhesion, look particularly for
articles by Andrade who was at University of Utah. If you
cross-search adhesion and the professor's name, you should be able to
find them.
Carol


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Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024 email: heckman-at-bgnet.bgsu.edu
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___________________________________________________________________________

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From: Rosey.VanDriel-at-csiro.au
Date: Sun, 20 Nov 2005 16:22:48 -0600
Subject: [Microscopy] TEM: HM20 problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
I have been using HM20 Monostep (premixed) resin for freeze substitution
for a couple of years, and have recently encountered problems with batch
variability. Sarah Ellis of the Peter MacCallum Cancer Clinic in
Melbourne has recently experienced similar problems.
The main problem is incomplete or variable infiltration, resulting in
polymerised resin pulling away from the edge of tissue blocks or cells,
leaving gaps and making sections hard to cut and to view. In some cases
there are gaps around larger organelles within the cells. Another batch
frosted badly at -45 degrees C.

My question to listserver members is whether anyone else is having these
variability problems. More importantly, does anyone have a solution?

Thanks,
Rosey van Driel


Rosemary van Driel
Electron Microscopy
New and Emerging Zoonotic Diseases
Australian Animal Health Laboratory
CSIRO Livestock Industries
Private Bag 24
Geelong Vic 3220
Australia

International Phone: +61 3 5227 5209
International Fax: +61 3 5227 5555
email: Rosey.VanDriel-at-csiro.au


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From: mcauliff-at-umdnj.edu
Date: Mon, 21 Nov 2005 10:32:48 -0600
Subject: [Microscopy] Re: TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm afraid that such a scanner would be very expensive. To scan the
full frame of a 3.25x4 inch EM negative you will need a 4x5 film
scanner, not an inexpensive item.
I suggest two alternatives. 1. A good flatbed scanner will be 1/10
the cost of a 4x5 film scanner. 2. Put your negatives on a light box
and photograph them with a digital camera. Most will focus very close,
then invert the image (negative to postitive) in PhotoShop.

Geoff

DRK-at-SHCC.org wrote:

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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: Rosey.VanDriel-at-csiro.au
Date: Mon, 21 Nov 2005 22:34:48 -0600
Subject: [Microscopy] TEM: HM20 problems: summary

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,
It would seem that our problems with Monostep HM20 may be due to aged or
contaminated resin.

The Monostep was supplied by a company which had its own labels on
otherwise unlabelled bottles. I have since learnt that the product is
shipped from the manufacturer with a lot number and expiry date. The
expiry dates are not on the product I received, which suggests there may
have been some rebottling or aging of the product. Interestingly, the
manufacturer was unable to trace the lot numbers printed on the
supplier's labels. The supplier refuses to entertain the thought that a
problem exists with the resin we recieved.

I have also discovered that resins ( and I don't know what else) can go
through 3 resellers before they reach us in our labs. So buyer beware!

I have traced a more direct source of supply, so I hope that the problem
is solved.

Thanks especially to the US commercial list subsciber who spared no
effort in helping track down the likely sources of the problem, I'll get
Alex to buy you a beer in Sydney in February. Thanks also to the other
supply companies in Australia who helped. I'd like to name the helpful
ones, but I don't think it's allowed.

Rosey



Rosemary van Driel
Electron Microscopy
New and Emerging Zoonotic Diseases
Australian Animal Health Laboratory
CSIRO Livestock Industries
Private Bag 24
Geelong Vic 3220
Australia

International Phone: +61 3 5227 5209
International Fax: +61 3 5227 5555
email: Rosey.VanDriel-at-csiro.au


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From: brakenho-at-science.uva.nl
Date: Tue, 22 Nov 2005 09:22:03 -0600
Subject: [Microscopy] FOM2006, Abstracts, Registration, Perth, Australia, April 9-12,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

FOCUS ON MICROSCOPY 2006, Perth, Australia, April 9-12, 2006
19th International Conference on 3D Image Processing in Microscopy
18th International Conference on Confocal Microscopy

2nd Announcement:

Dear Colleagues,

Abstracts for oral and poster presentations can now be submitted
preferably through the conference website:
http://www.focusonmicroscopy.org
where also the conference registration has opened and hotel
information is available.

Deadline for abstract submission: Jan. 9, 2006.
Note: this is earlier than in previous conferences!

The program will start on Sunday April 9, around 18 hours with an
opening symposium followed by a welcome reception.

After the successful FOM2005 conference in Jena, the next conference Focus
on Microscopy 2006 will take place in Perth, Western Australia, April
9-12, 2006. FOM2006 is the next in a series of unique interdisciplinary
meetings on advanced multidimensional light microscopy and image
processing. The conference will be hosted by the University of Western
Australia in Perth and held in the beautiful Esplanade Hotel in the
historic waterfront suburb of Perth, Fremantle.

Focus on Microscopy 2006 is the continuation of a conference series
presenting the latest innovations in optical microscopy and its
applications in biology, medicine, material science, and information
storage. 3D optical imaging and related theory are traditional subjects
for the conference. The conference series is also known for covering the
rapid development of advanced fluorescence labeling techniques for the
confocal andmulti-photon 3D imaging of -live- biological specimens.
This year, in addition, special attention will be given to imaging in
thick tissues.

Abstracts for contributions are invited and can now be submitted through
the website:

www.FocusOnMicroscopy.org

where further information on the present and previous FOM conferences can
be found.

Important dates:

Deadline for the submission of abstracts: January 9, 2006
Draft program available on the web: January 23, 2006
at website www.FocusOnMicroscopy.org
Deadline for early registration: February 20, 2006

Welcoming you to beautiful Perth for the FOM2006
conference and exhibition.

On behalf of the organizing committee,

David Sampson,
University of Western Australia, Perth, Australia

Fred Brakenhoff
Swammerdam Institute for Life Sciences, University of Amsterdam, The
Netherlands

E-mail: info2006-at-FocusOnMicroscopy.org

Web: www.FocusOnMicroscopy.org




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From: Kerstin.Brismar-at-vv.slu.se
Date: Tue, 22 Nov 2005 10:09:05 -0600
Subject: [Microscopy] SEM - Cryo system for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Cryo system for SEM, Oxford CT 1500B, bought in
1996, in excellent condition, used only three
times, will be sold for a very reasonable price.
A good opportunity to try the cryo technique, or
to get spare parts for an existing system. Don't
hesitate to ask for more information!


******************************
Kerstin Brismar
B.Sc., Research engineer, Photographer
Dept. of Crop Science
SLU (Swedish University of Agricultural Sciences)
P.O. Box 44 (Delivery: Växtskyddsvägen 1)
SE-230 53 Alnarp, Sweden
Phone: +46 40 41 55 05
Fax: +46 40 41 55 19
E-mail: Kerstin.Brismar-at-vv.slu.se
******************************



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From: mike.reedy-at-cellbio.duke.edu
Date: Tue, 22 Nov 2005 15:49:33 -0600
Subject: [Microscopy] TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Michael-
I checked your question with my Source, because I remembered only
that I had long shared your viewpoint, and that he had changed my
mind, but how....? So he reminded me . (The "they" he says set a
smaller DR aperture for negative scans than for positive transparency
scans are not Kodak, but scanner-makers in general, who rely on
Kodak's loooong experience):

"I know it is counterintuitive but it is true and can be easily
tested. In their haste to make scanning "better" they make and
assumption based on loooong experience. Kodak discovered that most
negatives have a transmission of 54% +- 5 %. They therefore set a
smaller aperture in the dynamic range to get a better scan. The
problem is that scientific imaging has a much wider need. Think
brightfield microscopy with 98% transmission or darkfield with 2%.
Because positive transparencies can have dark and light background
they open up the dynamic range to get better scans. In most scanners
scanning as a positive transparency and inverting will give you a
much better histogram than scanning as a negative.

Epson is ONLY scanner that I recommend; this is because the actual
calibrated resolution (I calibrated it myself) is 2000 dpi (4800dpi
claimed). This is half the possible resolution of film, that can
only be beaten with a 15,000 dpi drum scanner.

Our solution to a lack of film feeding is to put a scanner on every
desk. Scanning can be done while doing email/reading/ etc"

My own thought is-- if there were no such difference, why would
scanner controls continue to offer both options? ---- given that
"invert" has been available with a keystroke for some time, at least
since PhotoShop 2.0. I can think of possible simple tests, but I've
yet to perform any.

-mike reedy-


} Mike Reedy writes ...
}
}
} } [...]
} } My betters advise that it is better (more dynamic range/) to
} } scan as a transparency and invert rather than as a negative.
} } [...]
}
} I don't believe this is correct. How is better DR related to inverting the
} grayscale?
}
} genuinely :o)
} michael shaffer
}
} SEM/MLA Laboratory Coordinator
} (709) 737-6799 (ofc)
} (709) 737-6790 (lab)
} (709) 737-6193 (FAX)
} {www.mun.ca/creait/maf/}
}
} Inco Centre
} c/o Memorial University
} 230 Elizabeth Avenue
} P.O. Box 4200
} St. John's, NL A1C 5S7


--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html

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12, 20 -- From: Mike Reedy {mike.reedy-at-cellbio.duke.edu}
12, 20 -- Subject: RE: [Microscopy] Re: TEM negative scanner
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From: gcc-at-couger.com
Date: Tue, 22 Nov 2005 19:01:48 -0600
Subject: [Microscopy] Re: TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mike,

As you approach the resolution of film what problems do you see with
scanning the image from a random pattern of grain to the regular patter of
pixels. It obvious that straight line at angles other than the XY pattern
of the pixels are a problem but there are not that many straight lines in
most images.

With large pixels and small grain there is a loss of contrast does that
hold up as the pixel and grain size converge? As we are one or two
doublings of scanning resolution away from the point that digital is as
good or better than film the differences become more interesting.

With the drum scanners we have today one could scan in Kodak Tri X shot at
ISO 800 developed in HC 110 dilution B and be able to over sample the
grain on the negative for experemanal work. I think there are some photo
journalist who still shoot this combination. It has a unique look on news
print that some like.

I don't think there is anything standing in the way of higher resolution
drum scanners but the size of the image being 4 time larger for every
doubling in resolution and the computer power increasing by the square of
the time it takes to do operations. Combine that with the cost of making a
scanner to those tolerances and there is probably not a market for it.

Gordon
Gordon Couger

I collect links on information related to light microscopes.
www.couger.com/microscope/links/gclinks.html
Please forward anything you think might be useful to others.
Microscope Documentation is at www.science-info.org

mike.reedy-at-cellbio.duke.edu wrote:
} ----------------------------------------------------------------------------
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} Michael-
} I checked your question with my Source, because I remembered only
} that I had long shared your viewpoint, and that he had changed my
} mind, but how....? So he reminded me . (The "they" he says set a
} smaller DR aperture for negative scans than for positive transparency
} scans are not Kodak, but scanner-makers in general, who rely on
} Kodak's loooong experience):
}
} "I know it is counterintuitive but it is true and can be easily
} tested. In their haste to make scanning "better" they make and
} assumption based on loooong experience. Kodak discovered that most
} negatives have a transmission of 54% +- 5 %. They therefore set a
} smaller aperture in the dynamic range to get a better scan. The
} problem is that scientific imaging has a much wider need. Think
} brightfield microscopy with 98% transmission or darkfield with 2%.
} Because positive transparencies can have dark and light background
} they open up the dynamic range to get better scans. In most scanners
} scanning as a positive transparency and inverting will give you a
} much better histogram than scanning as a negative.
}
} Epson is ONLY scanner that I recommend; this is because the actual
} calibrated resolution (I calibrated it myself) is 2000 dpi (4800dpi
} claimed). This is half the possible resolution of film, that can
} only be beaten with a 15,000 dpi drum scanner.
}
} Our solution to a lack of film feeding is to put a scanner on every
} desk. Scanning can be done while doing email/reading/ etc"
}
} My own thought is-- if there were no such difference, why would
} scanner controls continue to offer both options? ---- given that
} "invert" has been available with a keystroke for some time, at least
} since PhotoShop 2.0. I can think of possible simple tests, but I've
} yet to perform any.
}
} -mike reedy-
}
}
}
} } Mike Reedy writes ...
} }
} }
} }
} } } [...]
} } } My betters advise that it is better (more dynamic range/) to
} } } scan as a transparency and invert rather than as a negative.
} } } [...]
} }
} } I don't believe this is correct. How is better DR related to inverting the
} } grayscale?
} }
} } genuinely :o)
} } michael shaffer
} }
} } SEM/MLA Laboratory Coordinator
} } (709) 737-6799 (ofc)
} } (709) 737-6790 (lab)
} } (709) 737-6193 (FAX)
} } {www.mun.ca/creait/maf/}
} }
} } Inco Centre
} } c/o Memorial University
} } 230 Elizabeth Avenue
} } P.O. Box 4200
} } St. John's, NL A1C 5S7
}
}
}


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From: microfrog9-at-hotmail.com
Date: Tue, 22 Nov 2005 20:29:08 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Phase Plate

Contents Retrieved from Microscopy Listserver Archives
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Email: microfrog9-at-hotmail.com
Name: Lisa Smith

Organization: University of Toronto

Title-Subject: [Filtered] Phase Contrast Microscope - Phase Plate

Question: What is the equation used to calculate the thickness of the etched region of the phase ring?

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: psneeley-at-xmission.com
Date: Tue, 22 Nov 2005 20:29:51 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: AO Spencer Series 4

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
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Email: psneeley-at-xmission.com
Name: Steve Neeley

Title-Subject: [Filtered] Looking for an AO Spencer Series 4 Microscope Reference Manual

Question: I have an AO Spencer Series 4 Microscope and need the Reference Manual. I have the Catalog, the Phase Catalog, and the Phase manual, but I need the actual reference manual that would have been provided for the scope by AO Spencer. These scopes were made in the 1950's and are blue/gray in color (Earlier scopes were the black Spencers and AO Spencers, later scopes were the Gray Series 10, etc.)

These microscope were heavily used at Colleges and Universities (many are still on the shelves, in labs, and in storage at least) and I'm hoping that someone has a copy of the manual.

If you have one, please contact me.

Thank you.

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From: pekysar-at-ucdavis.edu
Date: Tue, 22 Nov 2005 20:30:19 -0600
Subject: [Microscopy] viaWWW: Identifying motor endplates in thick sections

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Email: pekysar-at-ucdavis.edu
Name: Pat Kysar

Organization: University of California, Davis, School of Medicine,Pathology

Title-Subject: [Filtered] Identifying motor endplates in thick sections

Question: Hi all,
We have a pathologist who is conducting a study which necessitates the need to locate motor endplates in resin embedded muscle tissue. He would like to sucessfully locate them in thick sections so we can accurately cut thin sections which includes the area of interest.
Does anyone have a protocol for staining the endplates on the thick sections (epoxy resin embedded) to facilitate a more accurate identification of these structures? The pathologist has tried finding the areas on Methylene blue/Azure B stained sections with a very low rate of success and desires a more accurate method.
Any help would be appreciated.
Thanks,

Pat Kysar
EM Lab
University of California, Davis
School of Medicine, Pathology
1 Shields
Davis, CA 95616
pekysar-at-ucdavis.edu

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From: gary-at-gaugler.com
Date: Tue, 22 Nov 2005 23:21:45 -0600
Subject: [Microscopy] TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
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Diatribe aside....

What is your suggestion for a scanner?

Gary g.




At 05:03 PM 11/22/2005, you wrote:



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From: c.jeffree-at-ed.ac.uk
Date: Wed, 23 Nov 2005 02:47:49 -0600
Subject: [Microscopy] TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
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Depends on your objectives, I suspect.
If your objective is optimum combination of quality and versatility at the
lowest
cost then you can't go past Epson Photo scanners.
Epson 4870 Photo can generate embarassingly large files from an EM negative
which contain more detail than you can see on an 8"x10" photograph, and the
quality
of scans of 35mm transparencies can be good, more than adequate for 90% of
work in
scientific publishing. Having said that, photo buffs and EM people who are
looking for highest fidelity will
quickly notice limitations. DMax of 3.8 on 4870 (4.0 on 4990) looks good,
but in practice flare from the uncoated glass
platen compromises this performance. Images with extreme contrast (e.g. hard
black areas against pure white)
show visible and objectionable bleeding of light and colour into the dark
areas. I find illumination is not uniform, so that large images with
large areas of uniform density reproduce with density variation that is hard
to correct.
The 4870 is not really capable of resolving the grain of my transparencies,
and introduces a characteristic artefactual speckling or peppering of pixels
correlated with high-frequency information that can be objectionable when
images are enlarged to display sizes.
For this reason, I regard many of my scans of slides as adequate for
printing A4, but below par for archiving. If these things will worry you,
then you should probably
look at a dedicated film scanner such as one of the Nikon Coolscans. They
cost between 2 and 10 times the price,
but you get what you pay for.

Chris

----- Original Message -----
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To: {c.jeffree-at-ed.ac.uk}
Sent: Wednesday, November 23, 2005 5:22 AM


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From: keith.morris-at-ucl.ac.uk
Date: Wed, 23 Nov 2005 03:43:40 -0600
Subject: [Microscopy] TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
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Hi

As the chat on scanners is continuing I'll add my thoughts.

We use an 'old' semi-pro Agfa flatbed scanner that cost five thousand pounds
in its day - which runs on an Apple, as a Mac person bought it and
most hi-end graphics were then MAC orientated. Unfortunately Agfa,
Heidelberg and Fuji seem to have abandoned the pro scanner market, but the
hi-res scanning of many large negatives manually wasn't a problem for us, as
the scanner captures the images quite quickly and often we didn't require
full resolution for subsequent image analysis - in fact most time is spent
getting the images from Apple to IBM PC's. Apparently the cost of buying a
digital camera add-on for our units TEM would be around £70k with 2k x 2k
pixels and around £20k for 1k x 1k pixels (although our EM dept. has just
decided to go down the £70k route and dispense with film completely).

Creo is one of the few companies that I know that still make this type of
flat bed pro scanner, but I've no experience of their products. A scanner
that can take lots of 4x5" negatives across the whole of its scan area and
scan at 5000 dpi used to cost £10k +, and I have to admit I'm quite sure
what, if anything, has replaced them. As we have the original negative
anyway, 1600 dpi or less is fine for our digitising (mostly for subsequent
image analysis or incorporation into documents). Our scanner is naturally
used for slides, film, photo and paper copying as well.

You can still buy a few cheap sub £200 scanners with a light bulb area
larger than 35mm for transpancies, but all the best cheap ones seem fixed to
35mm slides/negatives. As mentioned by others, probably something like the
£750 Epson Expression 1680 Pro with its A4 transparency adapter would suite
for manual scanning - the A4 Transparency Unit "provides the capability to
scan multiple positive & negative films of up to 5"x4" in size" (the 1680
has 1600 x 3200dpi optical resolution). However we haven't got one to say
whether it is as good as our old Agfa (it's certainly a lot cheaper but
has similar specs). Things like hardware Digital ICE are probably less
useful as our negatives are in good condition (although it works well on my
home Benq Scanwit 2740S slide scanner that's used to scan very old colour
slides of the family). Negative/slide scanners do seem to have real problems
with grain size on my recent colour ASA 400 [and above] negatives generating
optical effects, but fortunately that isn't a problem we see with our TEM
negatives or my old Kodak/Agfa/Perutz 50 to 100 ASA slides from the
50s-70's.

Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk
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From: keith.morris-at-ucl.ac.uk
Date: Wed, 23 Nov 2005 04:18:11 -0600
Subject: [Microscopy] TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
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Hi

By the way, If you are 'archiving' the TEM negatives in maximum resolution
and possibly dumping the negative, I would buy the Nikon LS 900D - it's
£2,500 but I'm sure the smaller Nikon scanners are all 35mm only. Compared
to a digital camera on the TEM I suppose the price isn't bad. It can't scan
photo's or paper though. From my experience of scanning slides it can be a
daunting task working through an old archive at 3 minutes per photo just to
scan the TEM negative, but for new output that may be fine. It would be
great for my home slides though at 40s for 35mm.

Nikon details:

The SUPER COOLSCAN 9000 ED's multi-format capability is specifically
designed for imaging professionals. Scanning is possible for 120/220, 35mm,
6 x 7, 6 x 9 positives, 16mm, electron microscope and other film formats.
The 9000 ED's large-diameter Scanner Nikkor ED lens, 3-line CCD image sensor
and LED light source with rod dispersion have all been improved for enhanced
image quality with faster scanning speeds. These premium features give you
the leading edge in professional desktop imaging.


1. Multiple film formats (120/220, 35mm, etc.)
2. 4,000 dpi true optical resolution
3. 16-bit A/D converter
4. Large-diameter new Scanner Nikkor ED lens
5. Improved rod dispersion LED illumination
6. High-speed scanning (35mm slide film: 40 seconds; 6 x 9: 185 seconds)
7. Newly-developed, high-quality 3-line CCD sensor
8. New advanced image processing algorithm for colour negative film
9. Multi-sample scanning
10. Quick AF & Quick Preview
11. High-speed IEEE 1394 interface
12. Scan Image Enhancer
13. Digital ICE4 Advanced (TM) Digital ICE Quad Advanced) with Digital ICE
Professional(TM)

Regards
Keith

----------------------------------------------------------
Dr Keith J Morris
Imaging Facilities Manager
Cell Biology Division
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

Tel: 020 7608 4050
Fax: 020 7608 4034
email: keith.morris-at-ucl.ac.uk


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From: Edward.Calomeni-at-osumc.edu
Date: Wed, 23 Nov 2005 07:52:09 -0600
Subject: [Microscopy] viaWWW: Identifying motor endplates in thick sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all

can I just remind you that Microtek does produce the i900 which is a
large flatbed with full frame glassless holder much as the Agfa duoscan
systems were made. It has a true optical scan resolution of
3200x6400dpi (as far as I know - must be careful here because I know
there was a problem with the early Epsons), 4.2 dynamic range, scans up
to 8x10 inch transpareny or legal format for paper scans, its 48bit
colour USB 2.0 and firewire. It also comes with 'digital ice' and
Silverfast software. Check out the spec on:

http://www.microtek.nl/Product.php?
product=Detail&P_Id=107&Kword=i900&Select=All&FirstData=0

The only thing is that you would have to sort out your own e.m film
holder if it's not one of the following:
SnapTrans holders 35 mm slide holder; 35 mm filmstrip holder; 4"x5"
film holder; 6 x 9 cm film holder. I think it would be the same problem
for any/most of the other scanners. Ironically the do provide a glass
holder as well.

The current UK prices seem to be between about 700 uk pounds which
isn't bad for an 8x10inch glassless scanner - so you could be
talking 'gift-horse' and oral observation.

I don't actually have one but I have used the older Microtek scanmaker
8700 for a couple of years now without problem.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Sunderland
SR1 3SD
UK

e-mail: malcolm.haswell-at-sunderland.ac.uk




----- Original Message -----
X-from: keith.morris-at-ucl.ac.uk

Pat,

Not an easy task to accomplish. Back in the sixty's, the way to ID motor end
plates was to inject methylene blue into the muscle near the nerve while
still in the patient. A book "The Innervation of Muscle: A Biopsy Study" by
C. Coers and A.L. Woolf, published by Charles C. Thomas explains in detail
how this procedure is done.

If specimen is in blocks already, you might try doing immunohistochemistry on
the semi-thin sections using something like S-100 (or another nerve
antibody). Another idea may be to try enzyme histochemistry - with a PAP
secondary step - on the fixed tissue before embedding, using something like
cholinesterase.

I have never tried these, just some random thoughts.

Best of luck,

Ed




Edward P. Calomeni
Director EM Lab
The Ohio State University
M018 Starling Loving Hall
320 W. 10th Ave.
Columbus, OH 43210
614-293-5580 (office)
614-293-8806 (lab)
edward.calomeni-at-osumc.edu
-----Original Message-----
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Sent: Tuesday, November 22, 2005 9:38 PM
To: Edward Calomeni

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Email: pekysar-at-ucdavis.edu
Name: Pat Kysar

Organization: University of California, Davis, School of Medicine,Pathology

Title-Subject: [Filtered] Identifying motor endplates in thick sections

Question: Hi all,
We have a pathologist who is conducting a study which necessitates the need
to locate motor endplates in resin embedded muscle tissue. He would like to
sucessfully locate them in thick sections so we can accurately cut thin
sections which includes the area of interest.
Does anyone have a protocol for staining the endplates on the thick sections
(epoxy resin embedded) to facilitate a more accurate identification of these
structures? The pathologist has tried finding the areas on Methylene
blue/Azure B stained sections with a very low rate of success and desires a
more accurate method.
Any help would be appreciated.
Thanks,

Pat Kysar
EM Lab
University of California, Davis
School of Medicine, Pathology
1 Shields
Davis, CA 95616
pekysar-at-ucdavis.edu

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From: aolins-at-bowdoin.edu
Date: Wed, 23 Nov 2005 08:16:04 -0600
Subject: [Microscopy] viaWWW: digitizing negatives

Contents Retrieved from Microscopy Listserver Archives
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Email: aolins-at-bowdoin.edu
Name: Ada L. Olins

Organization: Bowdoin College

Title-Subject: [Filtered] digitize a negative

Question:

What are the most critical criteria to consider in order to optimize the quality of a
digitized em negative? Please recommend a scanner or features of a scanner.

I've searched the Listserver archives and not found an answer to my question.

---------------------------------------------------------------------------

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From: nathano72-at-gmail.com
Date: Wed, 23 Nov 2005 08:16:50 -0600
Subject: [Microscopy] viaWWW: centrifuge tubes?

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Email: nathano72-at-gmail.com
Name: Nathan O'Connor

Organization: Weill Medical College of Cornell University

Title-Subject: [Filtered] centrifuge tubes?

Question: Hi Everyone,

Don't know if such a thing exists (I didn't find anything via a quick search of a few vendors and google), but I'm trying to find centrifuge tubes that auto-open or vent during a spin. Any ideas?

Best regards,
Nathan

--
Nathan O'Connor
Blanchard Lab
Department of Physiology and Biophysics
Weill Medical College of Cornell University
1300 York Avenue
New York, NY 10021


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From: hyi-at-emory.edu
Date: Wed, 23 Nov 2005 08:21:31 -0600
Subject: [Microscopy] Re: TEM negative scanner

Contents Retrieved from Microscopy Listserver Archives
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If you all remember, I posted a similar message asking suggestions on
film scanners not long ago. I then did an extensive search myself, and
here is the result.

  First of all, I did not find too many professional grade film
scanners around that will do large format of standard TEM films. Imacon
models were the only ones I found that allows TEM film format, but they
cost over $12K. The first scanner I tried was Nikon SuperCool Scan
900. It has an optical resolution of 4000 dpi, and Dmax of 4.8. It is
perhaps one of the best film scanners for its price (around $2000), but
unfortunately the largest film format it will do is 6 X 8 cm. I looked
into whether or not the film carrier could be modified, but came to a
conclusion that if I really wanted to get this Nikon scanner, I would
have to trim my films, which I do not want to. I then bought an Epson
Perfection 4990 Photo. It is a flatbed about $450. The optical
resolution of this scanner is 4800 dpi, and Dmax 4.0. I consulted with
a few knowledgeable people, and they all think with specifications like
this, it should do an adequate job. But I did not like the images I got
from it at all. It lacks continuous gray tone, so images look contrast
and harsh. 

  The scanner I eventually purchased (about $1000) was Microtek
Artixscan 1800f. It is a dual media unit. The top glass is for
reflective scanning only, but it has a media insert holder that allow
two 4x5" negatives. I can mask off this holder to fit TEM negatives
in. This is better than using a flat bed with transparency adapter
since it eliminates Moire bands (Thank you, Gary Gaugler, for telling
me about this). This scanner offers the highest Dmax than any other
flatbed scanners I could find (Dmax 4.8). But the down side is its
limited resolution (1800 dpi). I debated for a while and decided it
will suit what we need. We usually view our images on a monitor, and
only print in small sizes for publication. We are now using this
scanner with our new Apple 20-inch flat panel cinema, and very happy
with it. However, I think another Microtek scanner, ScanMaker i900
might be a good choice too if not a better one. It cost $600. Rez, 3200
dpi; Dmax, 4.2. 

One thing I learned was that you cannot judge a scanner by its
specifications alone. You really need to try it out to see the end
result. Unfortunately the companies do not set up demos for scanners at
above price range. I end up returned twice and I am sure our purchasing
department was very happy with me. ;-)

Thank you, everyone, and to US readers, enjoy the holiday weekend.

Hong
Emory EM


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From: Elliott-at-arizona.edu
Date: Wed, 23 Nov 2005 11:10:21 -0600
Subject: [Microscopy] printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi listers
We have been discussing scanners. Thank you all for the input.

What are the current recommendations for printers? There are two
outputs I am interested in (one printer would be good, two is OK)
1) color micrographs
2) TEM micrographs

What do people use? What are you happy with?

Thank you

David


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From: gwe-at-ufl.edu
Date: Wed, 23 Nov 2005 12:26:33 -0600
Subject: [Microscopy] Insect EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Some time ago there was an interesting post describing a method that
allow the cuticle of an insect to bind to the resin so that they did not
separate during sectioning. I printed it out and filed it in a safe
place. now I cannot find it and clearly someone has stolen it. Does
anyone out there recall this post? The person treated the insect with
some stuff during embedding. Willing to pay big for this information.

Greg


-
Gregory W. Erdos, Ph.D,

Assistant Director, Biotechnology Program
Scientific Director, EM Core Lab
P.O. Box 118525
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
Phone: 352-392-1295
Fax: 352-846-0251


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From: W.Muss-at-salk.at
Date: Thu, 28 Apr 2005 09:01:02 -0500
Subject: [Microscopy] viaWWW: softening insect cuticle for histological

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Here you go:

best wishes,
Wolfgang Muss, Salzburg
X-from ListServer's Archive
---------------------------------------------------
X-from: Tindall, Randy D. : TindallR-at-missouri.edu

Some time ago there was an interesting post describing a method that
allow the cuticle of an insect to bind to the resin so that they did not
separate during sectioning. I printed it out and filed it in a safe
place. now I cannot find it and clearly someone has stolen it. Does
anyone out there recall this post? The person treated the insect with
some stuff during embedding. Willing to pay big for this information.

Greg


-
Gregory W. Erdos, Ph.D,

Assistant Director, Biotechnology Program
Scientific Director, EM Core Lab
P.O. Box 118525
University of Florida
Gainesville, FL 32611
gwe-at-ufl.edu
Phone: 352-392-1295
Fax: 352-846-0251


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From: chad.kritzberger-at-yale.edu
Date: Wed, 23 Nov 2005 13:11:29 -0600
Subject: [Microscopy] TEM - Problems with Trichoplax Fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello.

I am attemping to fix the small marine invertebrate Trichoplax adhaerens
for conventional TEM usage. My lab grows Trichoplax in large glass
petri dishes where they multiply by asexual budding and binary fission.
Prior to fixation, we transfer the animals to small glass wells with
filtered 36ppt seawater, where they move and lie flat on the bottom of
the glass wells after about 10-20 minutes.

So far, every fixative I've tried results in the animals displaying what
I call a "contraction response." About 3-5 seconds after rapid
immersion in a fixative, the large flat Trichoplax immediately contract
in an all-or-nothing response. They pull themselves inward, develop
frilled edges along their perimeter, and lift off the bottom of the
glass well temporarily before lying back down. Often, the animals curl
in on themselves and are fixed in that position. The degree of this
contraction response is variable, depending on how dissimilar the
fixative is to their normal seawater. For example, a very hypertonic
seawater solution (non-fixative) results in a severe contraction
response, as does seawater buffered in cacodylate. Most of the
fixatives I've tried are 4% glutaraldehyde based. My most successful
fixative is 4% glutaraldehyde in 36ppt seawater, no cacodylate, pH 8.3.
Nevertheless, this fixative causes the Trichoplax to undergo a mild
contraction response. Even introducing a fixative one drop at a time
results in the response.

In short, Trichoplax seems to be very sensitive to any changes in
external environment. The contraction is problematic for my TEM study,
so I am hoping to find a fixative that the animals do not respond to.
Do you have any suggestions regarding either the fixative solution or
the fixative protocol?

Thank you all in advance for your help - it is greatly appreciated!

Chad Kritzberger
chad.kritzberger-at-yale.edu
203-436-1538
Osborne Memorial Labs, Yale University

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From: allan.mitchell-at-stonebow.otago.ac.nz
Date: Wed, 23 Nov 2005 13:27:22 -0600
Subject: [Microscopy] Re: viaWWW: Identifying motor endplates in thick sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Pat

I don't know of a motor end plate stain that can be used on a resin
semi thin section. We would normally locate NMJ by doing a
cytochemical localisation before the processing and embedding.
However, in an 'unstained' muscle block I would look firstly for a
nerve bundle, then for a single nerve fibre running close to the muscle
bundle. If you can locate a single nerve fibre then there is a good
chance a NMJ is not far away.

You do have to develop a bit ofd an eye for them in the semi-thin
section. They are quite small in relation to the other structures and
even at high magn, can be very difficult to see. We describe them to
people looking for one along the edge of a muscle bundle as "look for a
section of the bundle edge that looks a little mottled, perhaps moth
eaten, compared to the rest of the edge. As I say, the single nerve
fibre is a good clue.

Good luck

Allan


On 23/11/2005, at 3:30 PM, pekysar-at-ucdavis.edu wrote:

}
}
}
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} Email: pekysar-at-ucdavis.edu
} Name: Pat Kysar
}
} Organization: University of California, Davis, School of
} Medicine,Pathology
}
} Title-Subject: [Filtered] Identifying motor endplates in thick
} sections
}
} Question: Hi all,
} We have a pathologist who is conducting a study which necessitates the
} need to locate motor endplates in resin embedded muscle tissue. He
} would like to sucessfully locate them in thick sections so we can
} accurately cut thin sections which includes the area of interest.
} Does anyone have a protocol for staining the endplates on the thick
} sections (epoxy resin embedded) to facilitate a more accurate
} identification of these structures? The pathologist has tried finding
} the areas on Methylene blue/Azure B stained sections with a very low
} rate of success and desires a more accurate method.
} Any help would be appreciated.
} Thanks,
}
} Pat Kysar
} EM Lab
} University of California, Davis
} School of Medicine, Pathology
} 1 Shields
} Davis, CA 95616
} pekysar-at-ucdavis.edu
}
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} 6, 12 -- From zaluzec-at-microscopy.com Tue Nov 22 20:30:18 2005
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}
}
Allan Mitchell
Otago Centre for Electron Microscopy
Department of Anatomy and Structural Biology
School of Medical Sciences
P.O. Box 913
Dunedin
New Zealand

Phone (03) 479 5642 or 479 7301
Fax (03) 479 5086 or 479 7254

EM Centre: http://ocem.otago.ac.nz/
Confocal Centre: http://occm.otago.ac.nz/
Department: http://anatomy.otago.ac.nz/


==============================Original Headers==============================
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From: lgarvie-at-asu.edu
Date: Wed, 23 Nov 2005 15:39:13 -0600
Subject: [Microscopy] SEM - amorphous C staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chad,

Have you tried cooling the critters first? In the 'frig at 4 deg C. This relaxes or slows many marine inverts so that they don't contract.
Also, MgCl2 is used as an anaesthetic. Try 0.37 M MgCl2.6H20, that should be isotonic to seawater (adjust as needed). This will take a few to maybe 15 minutes.
Or, try a few crystals of MgSO4. Relaxation is slower than with MgCl2, and more may need to be added after 1/2 hour or so.
There are also various funs chemicals to try, most of them narcotics. MS-222 (in the Sigma catalog) works for fish and small crustaceans, it may work on Trichoplax.
Gentle warming, CO2 (as seltzer water or bubbled in), and ethanol added dropwise also work for some animals.

Phil

Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576
Sic hoc legare scis nimium eruditionis habes.



-----Original Message-----
X-from: chad.kritzberger-at-yale.edu [mailto:chad.kritzberger-at-yale.edu]
Sent: Wed 05/11/23 14:15
To: Oshel, Philip Eugene

Hi all microscopists,

I need advice on making amorphous C visible in the SEM. Basically, I
have an uncovered thin section of a meteorite. This meteorite contains
many small 100 to 1000 nm amorphous C spheres. The C is composed of
small condensed aromatic units that are cross-linked with short
aliphatic chains. I would like to post stain the C so that I can
readily map and find the particles using a backscattered detector on
one of our FEG-SEMs.

I have no experience with sample staining - so of all the stains out
there, which one would be best for my sample?

Thanks,

Laurence



------------------------------------------------------------------------
--------------------------
Dr. Laurence A.J. Garvie
Faculty Research Associate
Department of Geological Sciences
Arizona State University
Tempe
AZ 85287-1404
USA

phone +480 965 0470
fax +480 965 8102
------------------------------------------------------------------------
---------------------------


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From: Colin.Veitch-at-csiro.au
Date: Wed, 23 Nov 2005 19:26:31 -0600
Subject: [Microscopy] Quantomix sample holders

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I noticed that JEOL are selling a product called the Quantomix capsule
which is a specimen holder which allows for the imaging of "wet" samples
in an SEM. I'd be interested in any reports on these as it is something
we are considering.

Cheers


Colin Veitch

Electron Microscopist

CSIRO Textile and Fibre Technology

PO Box 21, BELMONT, Vic. 3216. Australia.

E-mail: colin.veitch-at-csiro.au

Web: http://www.tft.csiro.au

Tel: +61 (0) 3 5246 4000
Mob: 0438 538 475
Fax: +61 (0) 3 5246 4811



The information contained in this e-mail message may be privileged or
confidential information. If you are not an intended recipient, you may
not copy, distribute or take any action in reliance on it. If you have
received this message in error, please telephone CSIRO Textile and Fibre
Technology on +61 3 5246 4000.



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From: jfactor-at-ns.purchase.edu
Date: Wed, 23 Nov 2005 21:22:12 -0600
Subject: [Microscopy] Re: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I just replaced an older Epson 820, which did a nice job of TEMs, with
nice neutral blacks when set on "black only", but with a somewhat thin
tonal range. HP seems to offer an advantage over Epson (the older ones,
at least), as you get a new print head each time you replace the
cartridge. The old Epson 820 (which has a print head build into the
printer, not in the cartridge), seemed very finicky to me, and needed
constant print head cleaning after a while. However, the older HPs made
grays by using color inks in addition to black, and always seemed to
leave a blue or purple tinge on the grayscale images.

The replacements are two similar, new HP printers, and just today I ran
some test prints of transmission electron micrographs. One 8-1/2x11
printer, the HP 8450, and a larger format printer, the HP 8750; the 8450
is moderately priced, but the 8750 is a bit pricey and seems to offer no
advantage besides the larger format for display prints. Both take three
cartridges (a typical tri-color cartridge, a photo cartridge [stay away
from the "photo blue" which is intended for intense blue skies and gives
TEMs a blue tinge], and a photo gray). It's the photo gray that gives
grayscale images a better tonal range, as it contains black (or dark
gray, I can't be sure which), middle gray, and light gray inks. When set
to "grayscale", with "black cartridge only" (the photo gray cartridge),
the HPs do a very nice job of it, both on Kodak Bright White paper for
drafts, and on high-gloss paper for final prints, and appear to give
very neutral grays. So far, so good (but it's only the first day).

--Jan Factor

---------------------------------------11/23/05
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College
State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------


Elliott-at-arizona.edu wrote:11/23/05
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} Hi listers
} We have been discussing scanners. Thank you all for the input.
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} What are the current recommendations for printers? There are two
} outputs I am interested in (one printer would be good, two is OK)
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} 2) TEM micrographs
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} What do people use? What are you happy with?
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} David
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From: David.Patton-at-uwe.ac.uk
Date: Thu, 24 Nov 2005 05:44:47 -0600
Subject: [Microscopy] TEM - Problems with Trichoplax Fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

No experience but.... I have read that osmium tetroxide can be used as a
primary fixative for marine invertebrates It might work faster as well.
Take care to use a sealed container (it is very volatile) if trying to
observe outside of a fume cupboard.

Dave

-----Original Message-----
X-from: chad.kritzberger-at-yale.edu [mailto:chad.kritzberger-at-yale.edu]
Sent: 23 November 2005 19:16
To: David Patton

Hello.

I am attemping to fix the small marine invertebrate Trichoplax adhaerens
for conventional TEM usage. My lab grows Trichoplax in large glass
petri dishes where they multiply by asexual budding and binary fission.
Prior to fixation, we transfer the animals to small glass wells with
filtered 36ppt seawater, where they move and lie flat on the bottom of
the glass wells after about 10-20 minutes.

So far, every fixative I've tried results in the animals displaying what
I call a "contraction response." About 3-5 seconds after rapid
immersion in a fixative, the large flat Trichoplax immediately contract
in an all-or-nothing response. They pull themselves inward, develop
frilled edges along their perimeter, and lift off the bottom of the
glass well temporarily before lying back down. Often, the animals curl
in on themselves and are fixed in that position. The degree of this
contraction response is variable, depending on how dissimilar the
fixative is to their normal seawater. For example, a very hypertonic
seawater solution (non-fixative) results in a severe contraction
response, as does seawater buffered in cacodylate. Most of the
fixatives I've tried are 4% glutaraldehyde based. My most successful
fixative is 4% glutaraldehyde in 36ppt seawater, no cacodylate, pH 8.3.
Nevertheless, this fixative causes the Trichoplax to undergo a mild
contraction response. Even introducing a fixative one drop at a time
results in the response.

In short, Trichoplax seems to be very sensitive to any changes in
external environment. The contraction is problematic for my TEM study,
so I am hoping to find a fixative that the animals do not respond to.
Do you have any suggestions regarding either the fixative solution or
the fixative protocol?

Thank you all in advance for your help - it is greatly appreciated!

Chad Kritzberger
chad.kritzberger-at-yale.edu
203-436-1538
Osborne Memorial Labs, Yale University

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From: hinmeigeng-at-hotmail.com
Date: Thu, 24 Nov 2005 06:32:50 -0600
Subject: [Microscopy] Contrast and focus in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi All!

I've just been looking at some thin crystals of an organic compound, sitting
on a carbon film, under bright-field TEM. When in exact focus, they appear
much less constrasty than when the focus knob is turned to the left of the
right. When turned to the right, dark fringes appear around the crystals.
I would like to ask:

(1) Which of the two , left or right, is over/under focus?

(2) Why are they more constrasty overall when over or under-focussed?

(3) Why are they more contrasty at lower magnification?

(Things were much easier when looking at shadowed-carbon replicas of polymer
surfaces!)

Any help would be much appreciated.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



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From: jb_sanderson-at-yahoo.com
Date: Thu, 24 Nov 2005 07:35:56 -0600
Subject: [Microscopy] 3 open positions for miscoscopists at the Max Planck Institute (MPI-CBG), Dresden, Germany

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

I would like to ask you for your help:

The Max Planck Institute of Molecular Cell Biology
and Genetics in Dresden, Germany is now advertising
for good people who would be willing to help us in
running core facilities in our international
institute ( more than 150 scientists from more then 30
countries). Currently there are three positions open:

Senior Microscopist (Imaging Specialist) - Light
Microscopy Facility
http://www.mpi-cbg.de/research/jobs/lmf.html

Facility Leader - Image Analysis Facility

http://www.mpi-cbg.de/research/jobs/imageanalysis.html

Facility Leader - Electron Microscopy Facility
http://www.mpi-cbg.de/research/jobs/em.html

If you are interested in these positions, please do
not hesitate to contact me.

If you know someone who might be also interested in
one of the above mentioned positions, please forward
my email to him/her. The deadline for the appliactions
is 9. December 2005.
Thank you very much for your kind assistance in
advance.

Regards

Jan

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Jan Peychl, M.D., Ph.D.
Service Leader
Light Microscopy Facility
Max Planck Institute of Molecular Cell Biology and
Genetics
Pfotenhauerstrasse 108
01307 Dresden
Germany

Tel.: +49 351 210 2502
Fax: +49 351 210 2000
web: www.mpi-cbg.de






___________________________________________________________
Yahoo! Messenger - NEW crystal clear PC to PC calling worldwide with voicemail http://uk.messenger.yahoo.com

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From: Philip.Koeck-at-biosci.ki.se
Date: Fri, 25 Nov 2005 02:27:08 -0600
Subject: [Microscopy] Contrast and focus in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Robert

I'm sure you will get much more detailed answers when the USA wakes up,
but here's my two pence worth.

When you turn to the right and see a dark fringe at the edge of objects
then it is overfocussed and a light fringe is underfocussed when you
turn to the left. These are Fresnel fringes and are produced because of
diffraction contrast which is a result
of scattering of light/electrons at edges. It can and is quite often
acceptable to defocus slightly to increase contrast but this should
normally only be slightly underfocussed. The logic being that bright
fringes will enhace the dark edges beside them, but dark fringes will
introduce more apparent dark structures.

When you examine specimens at low magnification amplitude contrast
makes a greater contribution to the image than phase contrast.
Amplitude contrast is generated by the
influence of the specimens atomic nuclei on electrons in the beam. The
higher the mass of the nucleus the greater the 'scattering power' of
that area of the specimen and the more electrons scattered out of the
main beam path the darker that area. Amorphous samples simply produce
more or less contrast by mass alone although crystals scatter electrons
in much more defined ways due to the interaction of the 'wavelength' of
the electrons, the lattice spacing in the crystal and its angle of tilt.

There are many
good books on this subject, but here's the first one that came to hand:
Principles and Practice of Electron Microscope Operation; A.W. Agar,
R.H. Alderson and D. Chescoe; Pub North Holland (3rd print 1980) ISBN 0
72044255 9 where chapter 3 on image formation is particularly
appropriate.

My apologies to crystallographers everywhere for a simple biologists
explanation of diffraction contrast without once mentioning Bragg.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Sunderland
SR1 3SD
UK
tel no: +44 (0)191 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: hinmeigeng-at-hotmail.com

Your description is right on target. When I was learning to use the
em (in the dark ages, with an RCA-EMU3), I was taught to obtain best
focus by experimenting first with a holey sample so that I could
adjust what my eyes thought was best focus (because we are uniquely
sensitive to contrast) to the true focus. I then would focus on a
sample to what I thought was best, and then adjust the focus setting
by a few notches. The issue of whether you want to include the
underfocus fringes in your image becomes, then, an issue of whether
you want to include the optical artifact for contrast, or you are
interested in the "true" edge of your objects.


Date sent: Thu, 24 Nov 2005 08:14:13 -0600
To: jbs-at-temple.edu
X-from: malcolm.haswell-at-sunderland.ac.uk
Send reply to: malcolm.haswell-at-sunderland.ac.uk

Hello,

I might be wrong, but seem to have a little different understanding from
the posts so far to these concepts of very importance in both theory and
practice so that I reply, hoping to solicit more insights. In short,

1) Slight under-focus (OL knob counterclockwise adjustment from the
least contrast or true focus point) not only makes image look good, but
provides better resolution.

2) There are only two types of contrast mechanisms in my opinion:
amplitude contrast and phase contrast. Amplitude contrast includes a)
diffraction contrast, b) mass-thickness contrast, and c) Z-contrast (a
special case of mass-thickness contrast).

Also due to the locality nature, Z-contrast and phase contrast can
normally be thought responsible for or capable of providing lattice
resolution (you may argue with in the cases of imaging with several
diffraction spots) or atomic resolution.

Happy holiday or vacation (to those who don't view Thanksgiving as a
holiday)!

Chaoying Ni
W.M. Keck Electron Microscopy Facility
Facility location: 022 Spencer Laboratory
Mailing address:
201 duPont Hall
Dept. of Materials Sci. and Eng.
University of Delaware
Newark, DE 19716
(302) 831-2318 (Phone) (302) 831-4545 (Fax)
http://eml.masc.udel.edu




-----Original Message-----
X-from: malcolm.haswell-at-sunderland.ac.uk
[mailto:malcolm.haswell-at-sunderland.ac.uk]
Sent: Thursday, November 24, 2005 9:16 AM
To: cni-at-UDel.Edu

Robert

I'm sure you will get much more detailed answers when the USA wakes up,
but here's my two pence worth.

When you turn to the right and see a dark fringe at the edge of objects
then it is overfocussed and a light fringe is underfocussed when you
turn to the left. These are Fresnel fringes and are produced because of
diffraction contrast which is a result
of scattering of light/electrons at edges. It can and is quite often
acceptable to defocus slightly to increase contrast but this should
normally only be slightly underfocussed. The logic being that bright
fringes will enhace the dark edges beside them, but dark fringes will
introduce more apparent dark structures.

When you examine specimens at low magnification amplitude contrast
makes a greater contribution to the image than phase contrast.
Amplitude contrast is generated by the
influence of the specimens atomic nuclei on electrons in the beam. The
higher the mass of the nucleus the greater the 'scattering power' of
that area of the specimen and the more electrons scattered out of the
main beam path the darker that area. Amorphous samples simply produce
more or less contrast by mass alone although crystals scatter electrons
in much more defined ways due to the interaction of the 'wavelength' of
the electrons, the lattice spacing in the crystal and its angle of tilt.

There are many
good books on this subject, but here's the first one that came to hand:
Principles and Practice of Electron Microscope Operation; A.W. Agar,
R.H. Alderson and D. Chescoe; Pub North Holland (3rd print 1980) ISBN 0
72044255 9 where chapter 3 on image formation is particularly
appropriate.

My apologies to crystallographers everywhere for a simple biologists
explanation of diffraction contrast without once mentioning Bragg.

Malcolm

Malcolm Haswell
e.m. unit
School of Health, Natural and Social Sciences
Fleming Building
University of Sunderland
Sunderland
SR1 3SD
UK
tel no: +44 (0)191 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk


----- Original Message -----
X-from: hinmeigeng-at-hotmail.com

Hi,

It seems about time to add a post from California (yes, some of us are
finally awake on the left coast).

As far as I can see, both (all) of the contributed points of view are
correct.

Underfocus by a few hundred Angstroms will indeed enhance contrast and
improve resolution. The explanation was first given by Otto Scherzer in
his famous "20/20" paper on page 20 of volume 20 of J. Appl. Phys.
[Scherzer, O. (1949). "The theoretical resolution limit of the electron
microscope" J. Appl. Phys. 20, 20-29].

Scherzer describes how an underfocus of minus sqrt (Cs * wavelength)
optimizes transfer of spatial frequencies into the image from the
electron wave leaving the specimen -- by balancing (positive) phase
shifts due to spherical aberration with (negative) shifts from
underfocus to form a kind of quarter-wave plate. Optimum underfocus
ranges from -750 Angstrom for a spherical aberration of 1 mm at 100 kV
to half that for a Cs of 0.5 mm at 300 kV.

Be aware that too much underfocus (or overfocus) will add too much
negative (or positive) phase shift and "scramble" the image so it no
longer shows a simple projection of the specimen.

There is more on high resolution and focus in many publications -- the
one I like is the paper "Resolution in high-resolution electron
microscopy", M.A. O'Keefe, Ultramicroscopy 47 (1992) 282-297. ;-)

For TEM of organic molecules see papers by John Fryer -- especially J R
Fryer 1993 J. Phys. D: Appl. Phys. 26 B137-B144. There's also M.A.
O'Keefe, J.R. Fryer and D.J. Smith, Acta Cryst. A39 (1983) 838-847.

Happy Thanksgiving,
Mike

Michael A. O'Keefe
National Center for Electron Microscopy
Lawrence Berkeley National Laboratory
Berkeley, CA 94720

----- Original Message -----
X-from: cni-at-udel.edu

p.s. If anyone would like a calculator program (PC only) that will
compute several TEM parameters, such as optimum defocus, let me know and
I'll email it to you. It uses reverse Polish -- to compute optimum
defocus for a voltage of 300kV and Cs of 1.2 mm, you would enter "1.2
300 wavl opt".
Mike
----- Original Message -----
X-from: MAOKeefe-at-lbl.gov

Hi,

You might want to have a look at a few useful webpages:

a focusing simulator:
http://www.umsl.edu/~fraundor/epc/index.html

contrast transfer function:
http://clik.to/ctfexplorer
http://ncmi.bcm.tmc.edu/homs/wen/ctf/ctfapplet.html



As we grow older we acquire wisdom,
but can't remember where we put it.- DA

Philip Koeck
Karolinska Inst. and University College of S. Stockholm
Dept. of Bioscience at Novum
tel: +46 8 608 9186
fax: +46 8 608 9290
web: http://www.csb.ki.se/users/philip/philown.html


-----Original Message-----
X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com]
Sent: 24 November 2005 13:37
To: Philip.Koeck-at-biosci.ki.se

Hi All!

I've just been looking at some thin crystals of an organic compound, sitting

on a carbon film, under bright-field TEM. When in exact focus, they appear
much less constrasty than when the focus knob is turned to the left of the
right. When turned to the right, dark fringes appear around the crystals.
I would like to ask:

(1) Which of the two , left or right, is over/under focus?

(2) Why are they more constrasty overall when over or under-focussed?

(3) Why are they more contrasty at lower magnification?

(Things were much easier when looking at shadowed-carbon replicas of polymer

surfaces!)

Any help would be much appreciated.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



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From: microfrog9-at-hotmail.com
Date: Fri, 25 Nov 2005 07:46:00 -0600
Subject: [Microscopy] viaWWW: Phase Contrast Microscope - Phase Plate

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Email: microfrog9-at-hotmail.com
Name: Lisa

Title-Subject: [Filtered] Phase Contrast Microscope - Phase Plate

Question: Hi,

Is there an equation to determine the depth of the etched region of a phase plate ?

Thanks

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From: neapit-at-yahoo.com
Date: Fri, 25 Nov 2005 08:15:49 -0600
Subject: [Microscopy] viaWWW:Microscopy & Molecular biology

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Email: neapit-at-yahoo.com
Name: Nea Pitulis

Organization: Medical school University of Athens-Greece

Title-Subject: [Filtered] Molecular biology

Question: Hi everybody.
I'm a PhD student in the med school with a degree in chemistry. I want to know where I can found info about microscopy techniques in the field of molecular biology.

thank you for helping me
Nea

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From: weis183-at-yahoo.fr
Date: Fri, 25 Nov 2005 12:46:21 -0600
Subject: [Microscopy] TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I'm looking for a reference sample for calibration
of camera lenght for diffraction and magnification
(mainly for High Resolution).
I already tried several sample (sold by Agar
scientific):

"Oriented single crystal gold foil" for camera
length and high resolution
"Evaporated aluminium film" for camera length
"Evaporated Thallous chloride" for camera length and
High Resolution
"Graphitised Carbon Black" for camera length and
High Resolution.

However I obtain some non negligible shift on
dspacings between various calibration standards
(around 1% or 2%) these shifts are reproducible and
thus are not due to alignement problem or bad
eucentric position.

As this sample are not sold with a control
certifical does someone know their real accuracy? Or
is their an explanation to this errors?

A more expensive calibration standard "MAG*I*CAL" is
sold, does someone has experienced it?

Any help would be much appreciated.

Patrick Weisbecker
LCTS / PESSAC
FRANCE







___________________________________________________________________________
Appel audio GRATUIT partout dans le monde avec le nouveau Yahoo! Messenger
Téléchargez cette version sur http://fr.messenger.yahoo.com

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From: tom-at-tomkaye.com
Date: Sat, 26 Nov 2005 22:45:32 -0600
Subject: [Microscopy] Robinson Detector Repair??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to start off with two questions.
Is there any way that you might have prepared this sample without embedding and preparing it as a thin section? How are you sure that such C spheres are present?
 
I expect that the C spheres would virtually disappear in the epoxy embedding medium which prompts your question about staining. If no epoxy was present, I suspect the spheres would show up nicely against the light background of the meteorite.
 
Since you cannot very well be using BSE to image the sample, how do you know the spheres are present and how do you know their chemical nature or size? If someone else has suggested or claimed their presence, how did they perform the characterization?
 
I would be interested in hearing the outcome of this exercise. It sounds like a challenge to do this by BSE even in a FEG-SEM, but I could be mistaken.
 
Warren Straszheim
Iowa State University

________________________________________
X-from: lgarvie-at-asu.edu [mailto:lgarvie-at-asu.edu]
Sent: Wed 11/23/2005 3:40 PM
To: wesaia-at-iastate.edu

Hello All,

I have a Robinson Detector that is problematic and in need of repair.
Strangely enough, a search of the web comes up blank on the manufacturer or
places that fix them.

Does anyone have contact info?

Great list by the way! As the rare hobbyist with an SEM, this is my only
contact with the EM world and it's much appreciated.

Tom Kaye




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From: cgarber-at-2spi.com
Date: Sun, 27 Nov 2005 08:35:40 -0600
Subject: [Microscopy] Robinson BSE detectors website

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tom Kaye wrote:
======================================================
Hello All,

I have a Robinson Detector that is problematic and in need of repair.
Strangely enough, a search of the web comes up blank on the manufacturer or
places that fix them.

Does anyone have contact info?

Great list by the way! As the rare hobbyist with an SEM, this is my only
contact with the EM world and it's much appreciated.
=====================================================
The website you are looking for is
http://www.etpsemra.com.au/

Dr. Vivian Robinson runs the company "hands on" and if you contact them
through their website in Australia, you could very well hear from Dr.
Robinson himself.

SPI Supplies has been providing sales and service for their detectors, see
URL
http://www.2spi.com/catalog/instruments/robinson-backscattered-electron-BSE-detector.shtml

Alternatively, you can contact me off-line and I will try my best to give
you the assistance you require.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================







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From: cgarber-at-2spi.com
Date: Sun, 27 Nov 2005 12:11:40 -0600
Subject: [Microscopy] =?Windows-1252?Q?Quantomix=AE_sample_holders?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colin Veitch wrote:
========================================================
I noticed that JEOL are selling a product called the Quantomix capsule
which is a specimen holder which allows for the imaging of "wet" samples
in an SEM. I'd be interested in any reports on these as it is something
we are considering.
========================================================
The Quantomix® capsules certainly do "work". I have seen them demonstrated
on several different occasions at trade shows and on the SEMs of several
different manufacturers. The technique is very exciting.

However I mention there is an alternative way to make an environmental (
vacuum compatible) cell, and that is with the use of silicon nitride
membrane window grids, two glued together, see URL
http://www.2spi.com/catalog/instruments/silicon-nitride.shtml

There is an example of the use of the SPI Supplies® Brand silicon nitride
membrane window grids toward the bottom of the page for an "environmental
cell" application.

Disclaimer: SPI Supplies offers a competing system to the Quantomix
capsules so we would have a vested interest in promoting the use of our SPI
Supplies® Brand silicon nitride membrane window grid system instead of the
polymeric window Quantomix capsules.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================







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From: schooley-at-mcn.org
Date: Sun, 27 Nov 2005 13:56:52 -0600
Subject: [Microscopy] children's microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's holiday shopping time again. Good books about magnification
written for the beginning reader are scarce. If your young one will
be getting a dissecting scope or a magnifying glass this year, there
are two new small, inexpensive pamphlets that you should consider:
"Looking Through a Microscope" & "You can Use a Magnifying Glass".
You'll find information on both in the MICRO bibliography (URL
below); enter "supplimental books", "for the primary grades", &
"recommended" in the search engine.
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

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From: pgrover-at-bilbo.bio.purdue.edu
Date: Mon, 28 Nov 2005 08:20:34 -0600
Subject: [Microscopy] $ 1 stocking stuffers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Surplus Shed (surplusshed.com) has folding doublet 10X magnifiers for $1.25
ea. (or 5/5.00). Glass lenses, aluminum & plastic housing. I like to drill
a hole through plastic hinge area to attach a neck string. Great stocking
stuffers, party favors, souvenirs of a field trip to your lab, or just carry
a few around in your pocket to give to youngsters or leave them in strategic
locations for someone to find (random act of kindness?). Price of a candy
bar. I'm ordering my second 100 of them. I reckon if it gets one kid in 10
away from the video game or TV for a little while, it's money well spent.

Disclaimer: I have no connection to Surplus Shed; I'm just cheap, and like
to pass along a 'steal' when I see one.

Paul

----------------------------------------------------------------------------
The world is so full of a number of things
I wonder we're not all as happy as kings

--Robert Louis Stevenson




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From: marko-at-wadsworth.org
Date: Mon, 28 Nov 2005 09:12:27 -0600
Subject: [Microscopy] Invitation to organize symposia for M&M2007

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

***Second announcement--confirmations will start end of December***

We are still looking for some great ideas for symposia at M&M2007!

Microscopy & Microanalysis 2007 Meeting

August 6-9, 2007
Broward County Convention Center
Ft. Lauderdale, Florida

Co-sponsored by

The Microscopy Society of America
The Microbeam Analysis Society
The International Metallographic Society

Proposals are coming in. We have suggestions for some of the customary
symposia, but have signed up only four organizers of these. Otherwise, the
program is still largely open at this time.

We would like the majority of the proposals to be submitted by the end of
the year. We will start sending out acceptance letters in late December,
and by mid-February we expect the program to be mostly filled.

This timetable is considerably accelerated in comparison with previous
years, and we now require a description of 150-300 words for each proposed
symposia. The description should take the form of those found in the Call
for Papers and Expo of past years; it should be an announcement of the
symposium and an invitation for contributions. The Program Committee will
select symposia based on these descriptions, so that overlap will be
minimized and symposia will complement each other to form a coherent
overall program.

You need not be a member of MSA, MAS, or IMS to propose a symposium,
although we hope that your experience with the M&M meeting will encourage
you to join.

Please send your suggestion (complete with description) directly to the
Program Chair, or to the M&M2007 Co-Chair of your Society.

The M&M2007 website is

http://mm2007.microscopy.org/

Early next year, symposia will be posted on the website as they are accepted.

Program contacts:

Mike Marko, Program Chair (marko-at-wadsworth.org)
John Henry Scott, Vice Program Chair (johnhenry.scott-at-nist.gov)
Ed Vincenzi, MAS Program Co-Chair (vicenzi-at-volcano.si.edu)
Steve Dekanich, IMS Program Co-Chair (dekanichsj-at-y12.doe.gov)

Local Arrangments contact:

Lucille Giannuzzi, Local Arrangements Chair (lgiannuzzi-at-feico.com)

Meeting Management contact:

Phillip Ridley, Conference Manager
Bostrom Corp
230 East Ohio, Suite 400
Chicago, IL 60611
Tel: 312-644-0828
Fax: 312-644-8557
Email: pridley-at-bostrom.com





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From: morgansbearhunter-at-yahoo.com
Date: Mon, 28 Nov 2005 10:56:38 -0600
Subject: [Microscopy] AskAMicroscopist: tem embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question was submitted to Ask-A-Microscopist by (morgansbearhunter-at-yahoo.com)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, November 28, 2005 at 10:49:22
Remember to consider the Grade/Age of the student when considering the Question
---------------------------------------------------------------------------
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Email: morgansbearhunter-at-yahoo.com
Name: Kathryn Privett

Organization: Charlotte Healthcare System

Education: Graduate College

Location: charlotte, NC,

Title: tem embedding

Question: Lately when I embed clinical tissue in blocks I get tiny little holes all over the tissue. I changed the dehydration Etoh, the propylene oxide. Now I am wondering if it could be the spurrs NSA. We ordered it and received in 2002. Do you have any suggestions?
Kathryn Privett

---------------------------------------------------------------------------

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From: milton.charlton-at-utoronto.ca
Date: Mon, 28 Nov 2005 11:25:07 -0600
Subject: [Microscopy] viaWWW: Jena operating scope parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
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Email: milton.charlton-at-utoronto.ca
Name: Milton Charlton

Organization: University of Toronto

Title-Subject: [Filtered] Jena operating scope parts

Question: Could anyone tell me where to get a replacement rack and pinion foucusing mechanism for a Jena operating scope that was made in the 1960's?
Thanks
M. Charlton
University of Toronto

---------------------------------------------------------------------------

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From: hinmeigeng-at-hotmail.com
Date: Mon, 28 Nov 2005 14:15:30 -0600
Subject: [Microscopy] Thanks (TEM) + Question (SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many thanks to y'all who replied to my question about under/over focus in
TEM. I think I have enough of an idea now.

Now for a SEM question. I've seen many questions about grain size of
sputtered gold on this list, but what about the tendency of gold films to
greak up into a sub-micron "crazy-paving" structure? Does anyone know what
causes this, and how to cure it? Often the crazy paving totally dominates
the picture, and one has to mentally filter it out to see the underlying
structure.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



==============================Original Headers==============================
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From: bozzola-at-siu.edu
Date: Mon, 28 Nov 2005 14:49:32 -0600
Subject: [Microscopy] Re: Thanks (TEM) + Question (SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One (prehaps the major) cause of the "crazy-paving" or "dried river
bed" appearance of specimens that were coated with heavy metals for
SEM viewing is the expansion and contraction of the underlying
specimen. Since the metal coating has little flex/stretch
capabilities, it will break (or shatter) if the underlying specimen
flexes or expands. The causes of the expansion of the specimen may
be: absorption/loss of moisture (as one goes in and out of the
vacuum), heating/cooling, mechanical flexing. This can be minimized
by keeping the specimens always in a dry environment, at a standard
temperature and not bending/flexing them.

} Now for a SEM question. I've seen many questions about grain size of
} sputtered gold on this list, but what about the tendency of gold films to
} greak up into a sub-micron "crazy-paving" structure? Does anyone know what
} causes this, and how to cure it? Often the crazy paving totally dominates
} the picture, and one has to mentally filter it out to see the underlying
} structure.
}
} -----------------------------------
} Robert H. Olley
} Reply to: R.H.Olley-at-reading.ac.uk
} URL: http://www.rdg.ac.uk/~spsolley
} -----------------------------------


--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
##############################################################

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From: walck-at-southbaytech.com
Date: Mon, 28 Nov 2005 15:04:37 -0600
Subject: [Microscopy] Thanks (TEM) + Question (SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The quick answer to your question concerning the structure of gold
coatings is to not use gold coatings. Gold is notorious for nucleating
islands on the surface that grow and eventually coalesce to form the
coating. This growth pattern results in your "crazy-paving" structure.
In fact, the resolution test sample for SEM has traditionally been a
gold coating evaporated onto a smooth carbon substrate and the minimum
distance between adjacent gold islands is used as the acceptance
measurement for the microscope. Au-Pd will offer a better coating on
inexpensive desktop coaters than plain gold, but you will still see the
grain structure at higher magnifications. Ion Sputter coating systems
such as our IBS/e system can sputter numerous different materials. The
materials that offer extremely good, uniform, and small structure
suitable for high resolution SEM work are Pt, Pd, Cr, Ir, and W.

If you look up Elaine Humphrey's response to a similar question on this
listserver from earlier this year, she gives a link to a web site that
shows comparisons of coatings from different materials using one of our
competitors' systems. (Sorry, I hope you understand why I don't give
out the URL.)

-Scott

Disclaimer: South Bay Technology, Inc. manufactures and sells the IBS/e
ion beam sputter deposition and etching system.

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: hinmeigeng-at-hotmail.com [mailto:hinmeigeng-at-hotmail.com]
Sent: Monday, November 28, 2005 12:23 PM
To: Walck-at-SouthBayTech.com


Many thanks to y'all who replied to my question about under/over focus
in
TEM. I think I have enough of an idea now.

Now for a SEM question. I've seen many questions about grain size of
sputtered gold on this list, but what about the tendency of gold films
to
greak up into a sub-micron "crazy-paving" structure? Does anyone know
what
causes this, and how to cure it? Often the crazy paving totally
dominates
the picture, and one has to mentally filter it out to see the underlying

structure.

-----------------------------------
Robert H. Olley
Reply to: R.H.Olley-at-reading.ac.uk
URL: http://www.rdg.ac.uk/~spsolley
-----------------------------------



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From: mike.reedy-at-cellbio.duke.edu
Date: Mon, 28 Nov 2005 16:35:40 -0600
Subject: [Microscopy] Re: AskAMicroscopist: tem embedding

Contents Retrieved from Microscopy Listserver Archives
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Kathryn
My guess is, the holes will go away if you use a
dehumidifier to bring the relative humidity in
your work-room (or work-chamber) below 60-70%
before, during and after the last change of 100%
accelerated resin. We used to get episodes of
holes in thin sections of our Araldite
506-DDSA-DER 736 resin mixture until we followed
the advice we found in this paper:

(1977) H. D. Dellman and C. Pearson Better
epoxy resin embedding for electron microscopy at
low relative humidity. Stain Technol. 52:5-8.

This is essential for us, perhaps moreso than for
most labs, because we need a dehumidified
environment during the 15-90 minutes it may take
us to lovingly manipulate fully infiltrated
single muscle fibers and rafts of 3-5 single
fibers into position on regions of dry substrate
with only a minimal micro-meniscus of external
resin; once thus positioned, they stick
themselves nicely to each other and to the smooth
substrate of polypropylene sheet (by surface
tension) during the first 2-4 hours of cure at
60° C. We then invert a full BEEM capsule of
liquid resin over the fiber or raft and finish
the cure overnight at 80°C. This gives us
superbly oriented single fibers exactly parallel
to the flat surface of the cured block and lying
within 3-5 microns of the resin surface; we like
to use it to co-embed fibers from different
experiments in a single raft, so we can make one
longitudinal (or cross-) section of a such a
combi-block do the work of 3-5 sections of
separate blocks.

The very high surface-to-volume ratio of this
"dry, flat" embedding procedure makes the resin
very susceptible to humidity during the pre-cure
manipulations. So we go for as low a humidity as
our commercial dehumidifier can achieve in our
small workroom, often below 50% if we run the
thing all night in a closed room. Obviously even
lower levels could be rapidly attained if the
dehumidified outflow from the appliance were
delivered into a small desktop working chamber,
but we've not found that to be necessary for our
work.

The curing oven itself is not a worry-- its
internal RH is below 10%, if i recall our few
measurements some years ago. So we typically
accelerate infiltration itself in 100%
accelerated resin mixture by putting the vials on
a sloped rotator in the 60°C oven, and
remembering to change the resin for fresh every
30 minutes, x2 or x3. In that heat, the resin
mixture becomes so water-thin, and stays that way
for at least 40 minutes, that I've long supposed
even Spurr's or similar could not become
significantly lower in viscosity or penetrate
the tissue any more completely.

-mike reedy-

At 11:04 AM -0600 11/28/05, morgansbearhunter-at-yahoo.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
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From: tivol-at-caltech.edu
Date: Mon, 28 Nov 2005 17:50:45 -0600
Subject: [Microscopy] Re: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Nov 25, 2005, at 10:46 AM, weis183-at-yahoo.fr wrote:

} A more expensive calibration standard "MAG*I*CAL" is
} sold, does someone has experienced it?
}
Dear Patrick,
I have used the MAG*I*CAL, and it works very well. You can calibrate
up to the highest mags on your scope using the Si lattice, and you can
calibrate camera lengths. If you have a tilt-rotation stage, you can
orient the MAG*I*CAL precisely to do all the calibrations, and with a
single-tilt stage you will have to orient the grid within the stage for
best results. The only problem with it is that it should be used at
room temperature, which is not a big deal except for cryo facilities.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: bozzola-at-siu.edu
Date: Mon, 28 Nov 2005 19:52:06 -0600
Subject: [Microscopy] Re: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I concur with Bill Tivol on the MAG*I*CAL calibration standard. We
have been using it since it first came out several years ago and have
been extremely pleased with it as it covers the entire magnification
range of the TEM and can be used to calibrate camera lenth, etc.

You need to be very careful with it (not for students to manipulate)
since it is brittle and easily broken. Yes, I broke one (a $900
mistake) when I put it back onto the membrane of the storage box. It
is best to allow it to drop gently onto the membrane since if you
misjudge the exact location of the membrane you can flex and break it.

Go for it and handle with care.

JB




} On Nov 25, 2005, at 10:46 AM, weis183-at-yahoo.fr wrote:
}
} } A more expensive calibration standard "MAG*I*CAL" is
} } sold, does someone has experienced it?
} }
} Dear Patrick,
} I have used the MAG*I*CAL, and it works very well. You can calibrate
} up to the highest mags on your scope using the Si lattice, and you can
} calibrate camera lengths. If you have a tilt-rotation stage, you can
} orient the MAG*I*CAL precisely to do all the calibrations, and with a
} single-tilt stage you will have to orient the grid within the stage for
} best results. The only problem with it is that it should be used at
} room temperature, which is not a big deal except for cryo facilities.
} Yours,
} Bill Tivol, PhD
} EM Scientist and Manager
} Cryo-Electron Microscopy Facility
} Broad Center, Mail Code 114-96
} California Institute of Technology
} Pasadena CA 91125
} (626) 395-8833
} tivol-at-caltech.edu
}
}
} ==============================Original Headers==============================
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--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Email: bozzola-at-siu.edu
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From: cgarber-at-2spi.com
Date: Mon, 28 Nov 2005 23:00:33 -0600
Subject: [Microscopy] Crazy paving structures in gold sputtered films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Robert H. Olley wrote:
========================================================
Now for a SEM question. I've seen many questions about grain size of
sputtered gold on this list, but what about the tendency of gold films
to greak up into a sub-micron "crazy-paving" structure? Does anyone know
what causes this, and how to cure it? Often the crazy paving totally
dominates the picture, and one has to mentally filter it out to see the
underlying structure.
========================================================
Our philosophy has always been that if this is happening, then you are
probably putting on too much gold and/or exposing the sample to too much
heat. Other factors can also increase the chances of seeing this kind of
effect, for example, the presence of a thin lubricant coating such as on a
storage media surface, catheter tubing or syringe needles. Additionally,
certain materials like PTFE are especially prone to this kind of cracking.
I have always assumed that this was at least in part due to the inherent
"grain" structure of any sputtered coating.

A layer of osmium metal deposited in an OPC osmium plasma coater, see URL
http://www.2spi.com/catalog/osmi-coat.html
which is not a sputtered coating, has no grain size (at least no one has
detected a grain size to our knowledge) and seems to be much more resistant
to the "crazy-paving" structure pattern effect on the above mentioned
samples. A comparison between different coating materials vs. osmium metal
is shown on URL
http://www.2spi.com/catalog/comparison-coating-results.html I propose
that the osmium coating approach would qualify for the requested "cure".

Disclaimer: SPI Supplies is the worldwide distributor of the OPC line of
osmium plasma coaters outside of Japan so we would naturally have a vested
interest in promoting the use of the osmium coaters for SEM sample
preparation.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================






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From: gary-at-gaugler.com
Date: Mon, 28 Nov 2005 23:35:03 -0600
Subject: [Microscopy] Re: Thanks (TEM) + Question (SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I've seen this "spider man" appearance before and attribute
it to sputtering at too high of pressure and too high of
current. Au is not a great metal for high resolution SEM
anyway...IMO. Au/Pd is better, and then consider Pt or Ir.

But it seems to me that low vacuum (15mT) and low current
(9mA) makes a big difference. But you will not likely get
15mT or lower without a turbo pumped system.

Try your system at the lowest vacuum you can get and see
what happens.

gary g.


At 12:20 PM 11/28/2005, you wrote:



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From: mike.reedy-at-cellbio.duke.edu
Date: Tue, 29 Nov 2005 16:14:07 -0600
Subject: [Microscopy] RE: Re: SEM imaging of bacteria encapsulation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
The 1991 BJ item is a meeting abstract, not article. It did cite the
prior work that inspired extensive trials we made of these methods in
the late 1980s.

Today I scanned it and a companion abstract about TAOS and TAURAC
fixation into a PDF and will separately email to you directly (no
idea how or if to transmit attachments via ListServer). My Acrobat 6
OCR function refuses to produce searchable text on these so they are
just images.; if i get an OCR to work, I will send that later.

David Popp and I planned to write up a methods paper with some
results but never finished the effort. We found that TAURAC-fixed
Araldite-embedded fibers of permeabilized, insect flight muscle
(fixed by TAURAC with a glutaraldehyde fixation interposed between
the TA and the UrAc fixes) still give fiber x-ray patterns showing
axial reflections out to 1.3 nm, and this result we will report in a
paper soon to be submitted by Kasim Sader (Univ. Leeds) et al.

The method can be cited from a 1994 peer-reviewed paper by H. Schmitz
et al., where it was described and some results illustrated in
Biophysical Journal (67:1620-1633).

-mike reedy-


At 10:26 AM -0600 11/29/05, Tindall, Randy D. wrote:
} Hi,
}
} I read this great post with great interest and have succeeded in
} tracking down two of the refs. I have been unable to locate the 1991
} Biophysical Journal article, however. Is the reference correct? Do you
} maybe have a PDF of this you could share? We have a client who is
} always looking for nifty ways to preserve biofilms and such.
}
} Thanks and Happy Holidays,
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
}
}
}
} -----Original Message-----
} From: mike.reedy-at-cellbio.duke.edu [mailto:mike.reedy-at-cellbio.duke.edu]
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--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
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From: jafarhan-at-rci.rutgers.edu
Date: Tue, 29 Nov 2005 20:32:45 -0600
Subject: [Microscopy] Atomic positions for WO2.9

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

I am doing some research with Tungsten Oxide. I got WO2.9 phase which is
tetragonal phase with space group of P4/nmm (from XRD). I wonder if
anybody have the atomic positions and Wykoff notations for this phase. I
have searched the literature but no luck.
Thank You

Jafar


==============================Original Headers==============================
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From: walck-at-southbaytech.com
Date: Wed, 30 Nov 2005 10:54:50 -0600
Subject: [Microscopy] Atomic positions for WO2.9

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I know that there are a number of WO3 and WO(3-x) compounds in the PDF
database from when I worked on the oxidation of WS2 thin films. Have
you checked whether this space group agrees with the WO3 phase? If it
does, then the positions are the same and you have to think of the x=.1
as oxygen vacancies that are distributed through the material.

-Scott

Scott D. Walck, Ph.D.
Technical Director
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

US Toll Free: 1-800-728-2233
Tel: (949) 492-2600
Fax: (949) 492-1499

www.southbaytech.com
walck-at-southbaytech.com


-----Original Message-----
X-from: jafarhan-at-rci.rutgers.edu [mailto:jafarhan-at-rci.rutgers.edu]
Sent: Tuesday, November 29, 2005 6:37 PM
To: Walck-at-SouthBayTech.com


Dear All,

I am doing some research with Tungsten Oxide. I got WO2.9 phase which
is tetragonal phase with space group of P4/nmm (from XRD). I wonder if
anybody have the atomic positions and Wykoff notations for this phase.
I have searched the literature but no luck. Thank You

Jafar


==============================Original
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From: mczech-at-sjm.com
Date: Wed, 30 Nov 2005 15:33:01 -0600
Subject: [Microscopy] viaWWW: Eye Damage by Light intensity?

Contents Retrieved from Microscopy Listserver Archives
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Email: mczech-at-sjm.com
Name: Mike Czech

Organization: St. Jude Medical, Inc

Title-Subject: [Filtered] Eye Damage by Light intensity

Question: Are there any studies that relate light intensity to eye damage from long term microscope usage?

---------------------------------------------------------------------------

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From: jmkrupp-at-cats.ucsc.edu
Date: Wed, 30 Nov 2005 18:36:10 -0600
Subject: [Microscopy] comparing images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have an unusual question, I will try to explain it as best I can.

A researcher has asked me to find out if it is reasonable to do anything
like a quantitative comparison between fluorescence images of different
samples.

She has thick sections, 20 um, on a confocal scope. She sees differences by
eye between control and treatments, and she would like to quantify these
differences in some way.

We have thought about setting up the confocal to record the brightest
image, then without changing the settings, record an image of the other
slides. The idea would be that she could do something like compare the
brightness levels between them.

I don't know enough about other options or if this will even work to help her.

Does anyone do anything like this or is this not realistic.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: donc-at-asmicro.com
Date: Wed, 30 Nov 2005 22:27:32 -0600
Subject: [Microscopy] SEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm soliciting the opinion of practicing SEM users regarding the materials
to use for a proposed high-precision 200 to 300 nm two-dimensional pitch
standard. The standard would be used to calibrate the horizontal scale (X
and Y axes) of a SEM or AFM. As I am an AFM expert, I already know what I
want in terms of the 3Dimension topography: the specimen will consist of an
array of bumps, with height probably 30-70 nm.
We are considering using etched silicon, silicon oxide on silicon, or
silicon nitride. Do any of these materials have advantages or disadvantages
for SEM use? Are some materials ok for low voltage ( { 1kV) and not ok for
high voltage?
Would it be a good idea to apply a metal coating? It seems obvious to me
that all magnetic materials should be avoided, such as Ni, Fe, Cr, Co. Are
there additional materials that are forbidden in some environments? For
example, I have heard that certain other metals are not allowed in some IC
fabs, but I don't know what they are.

I will be grateful to receive your input either via the list (which should
stimulate some good discussion) or offline.

regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]



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From: gary-at-gaugler.com
Date: Wed, 30 Nov 2005 22:54:09 -0600
Subject: [Microscopy] Re: SEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Look at the Geller 3 and 4 standards. They ought to
do what you need. If not, ask them for something else.

gary g.



At 08:47 PM 11/30/2005, you wrote:



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From: weis183-at-yahoo.fr
Date: Thu, 1 Dec 2005 01:32:50 -0600
Subject: [Microscopy] Thanks - TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for all answers about the MAG*I*CAL calibration
standard. It seems that it is worth the value even if
it must be handled with care (like most TEM thin
sections eventually).

Patrick Weisbecker
LCTS
PESSAC/FRANCE






___________________________________________________________________________
Appel audio GRATUIT partout dans le monde avec le nouveau Yahoo! Messenger
Téléchargez cette version sur http://fr.messenger.yahoo.com

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From: jrunions-at-brookes.ac.uk
Date: Thu, 1 Dec 2005 02:47:30 -0600
Subject: [Microscopy] Re: comparing images

Contents Retrieved from Microscopy Listserver Archives
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Hi Jon, the approach you describe to this problem would be sound if all
other factors were controlled and, in fact, we use it sometimes. The
problem with quantification of fluorescence is that there are so many
parameters to control and there is the non-linearity in fluorescence
emission to consider, i.e. is emission intensity linearly related to the
amount of fluorochrome present? Assuming that physical parameters
(preparation technique, section thickness, stain concentration /
fluorescent protein expression levels etc.) are controlled, you can
fairly easily say that one specimen is dimmer than another. One of the
most important caveats is to ensure that the emission levels in the
brighter image do not exceed 255. If they do, then you can not
reasonably say how bright the brightest point really was. Use a 'glow'
palette in the confocal software when setting gain levels to ensure that
the brightest specimen levels remain just below saturation. What do you
do next? The real trick is to describe why one specimen is brighter
than another. Either it will have to do with the relative amounts of
the stained tissue or the relative levels of gene expression. Both
developmental problems that can be solved genetically or biochemically.

Good luck, John.

jmkrupp-at-cats.ucsc.edu wrote:

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School of Biological and Molecular Sciences
Oxford Brookes University
Oxford, UK
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email: jrunions-at-brookes.ac.uk
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From: bfoster-at-mme1.com
Date: Thu, 1 Dec 2005 03:40:58 -0600
Subject: [Microscopy] Re: comparing images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

One way that you can do this is to use the Fluor ref slides to set your system to a specific intensity. If you have even a simple image analysis system, you should be able to take a point intensity reading. I'd recommend that you take it in the middle of the field since lamp to be consistent, since lamp alignment can have a big impact on eveness of illumination across the field.

The slides come in four different spectral responses, so you can probably find one close to the dye your colleague is using.

We have these slides at MME. You can arrange for purchase by contacting Ken Piel at (972)954-8011.

Hope this is helpful

Best regards,
Barbara Foster

CAVEAT: MME has a commercial interest in this product

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.



At 07:03 PM 11/30/2005, jmkrupp-at-cats.ucsc.edu wrote:



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From: nicholls-at-post.queensu.ca
Date: Thu, 1 Dec 2005 08:33:43 -0600
Subject: [Microscopy] Hitachi H500 TEM decommisioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Jonathon,

In theory measurement of the mean grey level of the relevant image region
should give a guide to the concentration of the fluorochrome marker and
hence what it is labelled to. Likewise total pixel brightness (sum of all
pixel grey levels within the region of interest) should give a guide to the
total mass of the fluorochrome marker and hence what it is labelled to. In
practice there may be differences in labelling efficiency, plus the
fluorescence may be quenched or bleached differently within the two samples,
so it's not an exact measurement. You would have to maximise the threshold
over the entire region of interest to include the darker areas, and
selection of the region of interest can bias the results if you aren't
careful. Also you could measure the total area of the pixels greater than a
set grey level (thresholded) to give a bit more info on differences.

The freeware Image J (Windows) or its Mac sister NIHImage
http://rsb.info.nih.gov/ij/ will do this for you, provided your images are
converted to standard TIF etc..formats it can read, although you may need a
few optional plug-ins (I use metaMorph and ImageProPlus here but they are
many £1,000s per licence). You would need to measure twenty of so different
images per group and do some t-test type stats to assess if there is any
significant difference (using Excels Stats add-ins). Naturally avoid any
post-capture image processing like brightness adjustment, sharpen etc.., and
the images should be collected at exactly the same confocal settings for
each fluorochrome - although confocal laser power, hardware and optics may
drift a little over time so try and take the alternate measurements of
control v exposed at the same sittings. The confocal pin-hole size and where
you take the z slice within the specimen can naturally also be important.

You can get involved with optical density, but that's really used with
densitometry measurements of light passing through tissue, and for that to
work you need densimetric metric standards of known density to calibrate the
transmission measurements (e.g. bone tissue sections with say polypropylene
and aluminium disks etc..). It's difficult to get fluorescence standards to
do a similar job for confocal fluorescence. Some suggest uranium glass
slides to check if the image capture is 'linear' across the field of view
(if you have one from the good old days). With regard to fluorescence
calibration standards, Molecular Probes make some fluorescent gel standards
that appear to suite. Alternatively you could try submicron fluorescent
beads at various known concentrations in gels or similar mountant. I use
Mattek dishes (a Petri dish with a hole cut-out and coverslip) with inverted
microscopes, which is a bit easier than using slides for these standards.
These standards can help check the 'linearity' of the confocal PMT detection
system.

Regards
Keith

PS. If you can have a look at Joece Loebl's Image Analysis - Principles and
Practice. They made the Magiscan B&W and colour Image analysers (before
Applied Imaging took them over) during the 1980's and early 1990's. It's
long out of print so its library loan only, but it is very clearly written
without to many equations.

----- Original Message -----
X-from: {jmkrupp-at-cats.ucsc.edu}
To: {keith.morris-at-ucl.ac.uk}
Sent: Thursday, December 01, 2005 1:26 AM

We have a Hitichi H500 that we are decommissioning. My question(s) is
how much oil is in the high tension tank and also does anyone still
use one and would they be interested in parts? Please reply to me
via list server or nicholls-at-post.queensu.ca

Rod Nicholls
Histology/Electron Microscopy Technician
Department of Anatomy and Cell Biology
Queen's University
Kingston, Ontario K7L 3N6
Phone: 613- 533 6000 x 78265


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From: Mike.Bode-at-soft-imaging.net
Date: Thu, 1 Dec 2005 08:21:13 -0600
Subject: [Microscopy] comparing images

Contents Retrieved from Microscopy Listserver Archives
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Hello Jon,

Any comparison between images is complicated, as in most cases the acquisition conditions are not or can not be controlled so as to give you identical conditions. This is even more difficult in fluorescence images, as the flourescence can bleach over time, so not only the conditions, but also the timing must be right.

The first problem you can try to tackle by embedding some standards into the preparation. Perhaps some small fluorescent beads that you can then use to normalize the image. Instead of using the intensity itself, you would use the intensity ratio of sample/bead as a measure.

If your samples bleach, you can try to do severl things:

1) acquire a time series of each sample and measure the decay of the signal and extrapolate to time 0, or
2) acquire the image at precise times, for example 1 minute after turning on the illumination.

Good luck.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
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fax:    (303) 234-9271
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-----Original Message-----
X-from: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Wednesday, November 30, 2005 6:06 PM
To: Mike Bode

I have an unusual question, I will try to explain it as best I can.

A researcher has asked me to find out if it is reasonable to do anything like a quantitative comparison between fluorescence images of different samples.

She has thick sections, 20 um, on a confocal scope. She sees differences by eye between control and treatments, and she would like to quantify these differences in some way.

We have thought about setting up the confocal to record the brightest image, then without changing the settings, record an image of the other slides. The idea would be that she could do something like compare the brightness levels between them.

I don't know enough about other options or if this will even work to help her.

Does anyone do anything like this or is this not realistic.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: phillipst-at-missouri.edu
Date: Thu, 1 Dec 2005 13:15:33 -0600
Subject: [Microscopy] GFP & Triton X-100

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know if GFP fluorescence survives when formaldehyde-fixed
tissues are permeabilized with Triton X-100? I need to double label a GFP
expressing tissue. thanks, tom



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: nicholls-at-post.queensu.ca
Date: Thu, 1 Dec 2005 16:07:58 -0600
Subject: [Microscopy] Hitachi H500 TEM decommisioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have a Hitichi H500 that we are decommissioning. My question(s) is
how much oil is in the high tension tank and also does anyone still
use one and would they be interested in parts? Please reply to me
via list server or nicholls-at-post.queensu.ca

Rod Nicholls
Histology/Electron Microscopy Technician
Department of Anatomy and Cell Biology
Queen's University
Kingston, Ontario K7L 3N6
Phone: 613- 533 6000 x 78265


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From: mike.reedy-at-cellbio.duke.edu
Date: Thu, 1 Dec 2005 11:49:03 -0600
Subject: [Microscopy] Re: AW: AW: SEM imaging of bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dr Muss-
Thanks for the paper and information about your uses of TA.

(The inserted PDF attachment you sent came to me
through the list-server. How did that succeed?
I will try here to to insert my PDF of the 1991
abstract (see below) and see if it passes to
become available to other readers.)

I have been meaning for some time to try TA as a
first-step section stain, as suggested by some
papers of ~15 years ago (At the moment i can
track down
Haldar et al (J Clin Pathol, (1992),
45:633-635; use a MIX of TA + UrAc!) and
Stirling (J.Histochem.Cytochem. (1993) 41:643-648.)
Maybe I will finally do so, now that I know your procedure.

I'm interested that you keep it in the dark as a
prepared solution. When we started using TA with
GA in early 1979 (inspired by David Begg et al
paper in
J Cell Biol (1978) 79:846-852
we soon got the idea that we could not gain the
benefits if improved contrast and better
preservation if we stored TA as a prepared or
stock solution. The color changed a bit after
1-2 days, became darker I think, and the
structural preservation was not so nice. Ever
since, we make it a habit to dissolve TA fresh,
whether using it in a mix with GA or simply alone
as primary fix, starting the fix no later than
about 3 hours after dissolving the TA, same
precaution whether in aqueous or
cryo-substitution-acetone procedures. Perhaps
keeping it in the dark might prolong its useful
life in solution, but because we lost 2+ weeks of
work back in 1979 before we learned our lesson,
we chose after that not to explore the use of
aged TA solutions.

The TA and TAMD dendromer paper you sent is
interesting. We are at the moment more
interested in low low MW mimics of TA like
catechin, because even the best low MW TA seems
unable to penetrate and carry stain mordanting
properties to the interior of globular protein
domains-- even if the UrAc or OsO4 is applied
before the TA it does not attract the TA to the
interior of myofilaments. The result is some
degree of negative staining on the smallest
scale; it appears to produce a dense nanoshell
around actin monomers and myosin head globular
domain in our most detailed EM tomography of thin
sections from cryo-substituted insect flight
muscle fibers
(J Struct Biol. (2004) 147:268-282).
(We have several more detailed and convincing
density-contoured images than the published
figure.)
This shell remains more dens than any interior
positive staining that may also occur, despite
the very intense section staining produced by
the permanganate -} Sato's Pb stain sequence we
use. We suppose that TA is probably ALWAYS
producing a surface coat on globular protein
domains, regardless of whether it also may
penetrate. Using catechin instead sometimes
seemsed to make the staining more positive (by
eye, not tomography) in some regions of sections
from a muscle fiber so treated. TA may produce
or allow more permeating and homogeneous staining
of very slender 2-4 nm molecular strands like the
lever-arm of the myosin heads, and even of much
fatter structures, such as the M-region of thick
filaments which often appear a glassy uniform
gray despite the nano-negative staining of the
adjacent thin filaments and the A-band.

All of this is to say why we are not immediately
interested in trying the TA dendromers, and why
we need to try TA (and probably also catehin) in
a section staining procedure. Perhaps at the cut
surface of a section it can penetrate internal
regions of actin monomers and myosin heads and
filament backbones that proved rather
impenetrable when exposed to TA in liquid
fixatives.

-mike reedy-

you must double-click on the apparently blank pdf
just below to make it open in Acrobat and show
its content.



}
}
}
} Dear Dr. Reedy,
}
} I would like to thank you very much for your copy "service".
} These abstracts I gladly shall integrate into my "library of EM-methods".
}
} I found TA (if "low molecular weight") very helpful in stabilizing
} structures and demonstrating structure usually not "included" in stained
} sections if processed the "normal" schedule (FA-GA,GA,OsO4,Dehydration).
}
} Also we (in a diagnostic EM-Lab) use this stuff within a pre-incubation
} step (0.05-0.5% w/v hydrous solution, sonicated, filtered and stored in
} plastic syringes in the dark, dispensed via a 0.25 or 0.42 ?m millipore
} filternotch to grids, 5-15 min {usually} at room temperature), before
} applying the classical UO2Ac-Lead-citrate staining sequence on to the
} ultrathin sections. Then we will have (depending on application time, and
} temperature, respectively) a very discrete to heavily e-dense staining of
} e.g. collagen fibrils, glycogen (+/-"specifically") and some extracellular
} matrix components usually not seen without that pretreatment.
}
} That effect is/will be increased by using a } para-phenylenediamine
} (PPD) {step after osmication (i.e. OsO4 as usual, washing in buffer, EtOH
} 50%, followed by an incubation of tissue samples in 1% PPD in 70%EtOH for
} about 25-30 min-at-rtemp, washing several times unless solution is clear,
} further dehydration-processing steps as usual).
} Additionally, I found that such a treatment generally results in a better
} stabilization of diseased tissue and therefore also better visualisation of
} most tissue components at the ultrastructural level.
}
} I would like to send to you a paper as .pdf you perhaps might not have in
} your library.
} The article does not describe TA } used as a primary fixative in EM {, but,
} instead, tells us about the mechanisms of effects exerted by TA-and
} TA-mimicking dendrimers on to mucosal scaffolds for transplantations, as
} evaluated also by TEM......interesting work.....
}
}
} Best regards and wishes to you and yours,
}
} Wolfgang Muss
} Salzburg/Austria
}
} ----------
} Von: Mike Reedy[SMTP:mike.reedy-at-cellbio.duke.edu]
} Gesendet: Mittwoch, 30. November 2005 16:37
} An: W.Muss-at-salk.at
} Betreff: Re: AW: [Microscopy] RE: Re: SEM imaging of bacteria
} encapsulation
}
} { {Datei: 1991 BJ Abstracts -at- TAURAC, TAOS.pdf} }
}
}
}
}
} Dear Dr Muss,
}
} Thank you for the kind holiday wishes. May yours be also wonderful!
} Here is the PDF, with my hopes you find the method useful.
}
} -mike reedy-
}
}
} } Good morning,
} }
} } dear Dr. Reedy,
} }
} } I greatly should appreciate receiving by e-mail the .pdf on the
} } TAURAC-methods mentioned below (which by no means you will be able to
} } transmit via the Listserver because any attachment will be blocked and
} } deleted).
} }
} } Have a nice and calm "advent-" and a beautiful Christmas time,
} } yours thankfully
} }
} }
} } Wolfgang Muss PhD
} } EM-Lab., PATHOLOGY SALK
} } SALZBURG, Austria
} }
} }
} }
} }
} } ----------
} } Von: mike.reedy-at-cellbio.duke.edu[SMTP:mike.reedy-at-cellbio.duke.edu]
} } Antwort an: mike.reedy-at-cellbio.duke.edu
} } Gesendet: Dienstag, 29. November 2005 23:22
} } An: W.Muss-at-salk.at
} } Betreff: [Microscopy] Re: SEM imaging of bacteria encapsulation
} }
} } ------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html


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16, 21 -- encapsulation; tannic
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From: cadr-at-mba.ac.uk
Date: Thu, 1 Dec 2005 16:11:16 -0600
Subject: [Microscopy] viaWWW: Immunogold and marine larvae

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Email: cadr-at-mba.ac.uk
Name: catherine

Organization: MBA

Title-Subject: [Filtered] Immunogold and marine larvae

Question: Hello,

I would like to perform an immunogold (TEM) on marine larvae.
I am looking for some basic protocols.. .

Any help and advices will be appreciated .

Thanks a lot.

Catherine




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From: kjl226-at-vt.edu
Date: Thu, 1 Dec 2005 08:51:28 -0600
Subject: [Microscopy] viaWWW: Request for Vendors to send Info on TEM's

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Email: kjl226-at-vt.edu
Name: Kathy Lowe

Organization: Virginia Tec h, College of Vet. Med

Title-Subject: [Filtered] New TEM information

Question: I am interested in getting information from companies on their Transmission Electron Microscopes. We have a old Zeiss 10CA. I'm just interested in learning more about what's available. We deal mainly with biological samples with the exception of a few polymer samples.

I would appreciate any information.

---------------------------------------------------------------------------

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From: beth-at-plantbio.uga.edu
Date: Thu, 1 Dec 2005 16:14:27 -0600
Subject: [Microscopy] Nikon DS -5M camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Don,
The only comment that I might make is that silicon nitride and silicon are
conductive, so they are suitable for SEM as is, whereas silicon oxide on
silicon is an insulator and may cause charging problems in the SEM. Also, at
low kVs the pure silicon will have a thin oxide layer that may also cause
charging problems. Etching also tends to induce an oxide layer. Coating the
sample will change its dimensions slightly.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
X-from: {donc-at-asmicro.com}
To: {mager-at-interchange.ubc.ca}
Sent: Wednesday, November 30, 2005 9:17 PM

Hi all,
Does anyone have the Nikon DS - 5M camera on their light microscope?
Is there a way to format the CF card with the DS Camera Control Unit
DS-L1?

Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



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From: phillipst-at-missouri.edu
Date: Thu, 1 Dec 2005 16:21:10 -0600
Subject: [Microscopy] GFP & Triton X-100

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know if GFP fluorescence survives when formaldehyde-fixed
tissues are permeabilized with Triton X-100? I need to double label a GFP
expressing tissue. thanks, tom



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: kjl226-at-vt.edu
Date: Thu, 1 Dec 2005 16:24:32 -0600
Subject: [Microscopy] viaWWW: Request for Vendors to send Info on TEM's

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Email: kjl226-at-vt.edu
Name: Kathy Lowe

Organization: Virginia Tec h, College of Vet. Med

Title-Subject: [Filtered] New TEM information

Question: I am interested in getting information from companies on their Transmission Electron Microscopes. We have a old Zeiss 10CA. I'm just interested in learning more about what's available. We deal mainly with biological samples with the exception of a few polymer samples.

I would appreciate any information.

---------------------------------------------------------------------------

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From: Mike.Bode-at-soft-imaging.net
Date: Thu, 1 Dec 2005 16:28:01 -0600
Subject: [Microscopy] comparing images

Contents Retrieved from Microscopy Listserver Archives
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Hello Jon,

Any comparison between images is complicated, as in most cases the acquisition conditions are not or can not be controlled so as to give you identical conditions. This is even more difficult in fluorescence images, as the flourescence can bleach over time, so not only the conditions, but also the timing must be right.

The first problem you can try to tackle by embedding some standards into the preparation. Perhaps some small fluorescent beads that you can then use to normalize the image. Instead of using the intensity itself, you would use the intensity ratio of sample/bead as a measure.

If your samples bleach, you can try to do severl things:

1) acquire a time series of each sample and measure the decay of the signal and extrapolate to time 0, or
2) acquire the image at precise times, for example 1 minute after turning on the illumination.

Good luck.

mike


Michael Bode, Ph.D.
Soft Imaging System Corp.
12596 West Bayaud Avenue
Suite 300
Lakewood, CO 80228
===================================
phone:  (888) FIND SIS
        (303) 234-9270
fax:    (303) 234-9271
email:  mailto:info-at-soft-imaging.com
web:    http://www.soft-imaging.com
===================================


-----Original Message-----
X-from: jmkrupp-at-cats.ucsc.edu [mailto:jmkrupp-at-cats.ucsc.edu]
Sent: Wednesday, November 30, 2005 6:06 PM
To: Mike Bode

I have an unusual question, I will try to explain it as best I can.

A researcher has asked me to find out if it is reasonable to do anything like a quantitative comparison between fluorescence images of different samples.

She has thick sections, 20 um, on a confocal scope. She sees differences by eye between control and treatments, and she would like to quantify these differences in some way.

We have thought about setting up the confocal to record the brightest image, then without changing the settings, record an image of the other slides. The idea would be that she could do something like compare the brightness levels between them.

I don't know enough about other options or if this will even work to help her.

Does anyone do anything like this or is this not realistic.

Thanks

Jon

Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu



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From: mike.reedy-at-cellbio.duke.edu
Date: Thu, 1 Dec 2005 16:37:14 -0600
Subject: [Microscopy] Re: AW: AW: SEM imaging of bacteria

Contents Retrieved from Microscopy Listserver Archives
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Dr Muss-
Thanks for the paper and information about your uses of TA.

(The inserted PDF attachment you sent came to me
through the list-server. How did that succeed?
I will try here to to insert my PDF of the 1991
abstract (see below) and see if it passes to
become available to other readers.)

I have been meaning for some time to try TA as a
first-step section stain, as suggested by some
papers of ~15 years ago (At the moment i can
track down
Haldar et al (J Clin Pathol, (1992),
45:633-635; use a MIX of TA + UrAc!) and
Stirling (J.Histochem.Cytochem. (1993) 41:643-648.)
Maybe I will finally do so, now that I know your procedure.

I'm interested that you keep it in the dark as a
prepared solution. When we started using TA with
GA in early 1979 (inspired by David Begg et al
paper in
J Cell Biol (1978) 79:846-852
we soon got the idea that we could not gain the
benefits if improved contrast and better
preservation if we stored TA as a prepared or
stock solution. The color changed a bit after
1-2 days, became darker I think, and the
structural preservation was not so nice. Ever
since, we make it a habit to dissolve TA fresh,
whether using it in a mix with GA or simply alone
as primary fix, starting the fix no later than
about 3 hours after dissolving the TA, same
precaution whether in aqueous or
cryo-substitution-acetone procedures. Perhaps
keeping it in the dark might prolong its useful
life in solution, but because we lost 2+ weeks of
work back in 1979 before we learned our lesson,
we chose after that not to explore the use of
aged TA solutions.

The TA and TAMD dendromer paper you sent is
interesting. We are at the moment more
interested in low low MW mimics of TA like
catechin, because even the best low MW TA seems
unable to penetrate and carry stain mordanting
properties to the interior of globular protein
domains-- even if the UrAc or OsO4 is applied
before the TA it does not attract the TA to the
interior of myofilaments. The result is some
degree of negative staining on the smallest
scale; it appears to produce a dense nanoshell
around actin monomers and myosin head globular
domain in our most detailed EM tomography of thin
sections from cryo-substituted insect flight
muscle fibers
(J Struct Biol. (2004) 147:268-282).
(We have several more detailed and convincing
density-contoured images than the published
figure.)
This shell remains more dens than any interior
positive staining that may also occur, despite
the very intense section staining produced by
the permanganate -} Sato's Pb stain sequence we
use. We suppose that TA is probably ALWAYS
producing a surface coat on globular protein
domains, regardless of whether it also may
penetrate. Using catechin instead sometimes
seemsed to make the staining more positive (by
eye, not tomography) in some regions of sections
from a muscle fiber so treated. TA may produce
or allow more permeating and homogeneous staining
of very slender 2-4 nm molecular strands like the
lever-arm of the myosin heads, and even of much
fatter structures, such as the M-region of thick
filaments which often appear a glassy uniform
gray despite the nano-negative staining of the
adjacent thin filaments and the A-band.

All of this is to say why we are not immediately
interested in trying the TA dendromers, and why
we need to try TA (and probably also catehin) in
a section staining procedure. Perhaps at the cut
surface of a section it can penetrate internal
regions of actin monomers and myosin heads and
filament backbones that proved rather
impenetrable when exposed to TA in liquid
fixatives.

-mike reedy-

you must double-click on the apparently blank pdf
just below to make it open in Acrobat and show
its content.



}
}
}
} Dear Dr. Reedy,
}
} I would like to thank you very much for your copy "service".
} These abstracts I gladly shall integrate into my "library of EM-methods".
}
} I found TA (if "low molecular weight") very helpful in stabilizing
} structures and demonstrating structure usually not "included" in stained
} sections if processed the "normal" schedule (FA-GA,GA,OsO4,Dehydration).
}
} Also we (in a diagnostic EM-Lab) use this stuff within a pre-incubation
} step (0.05-0.5% w/v hydrous solution, sonicated, filtered and stored in
} plastic syringes in the dark, dispensed via a 0.25 or 0.42 ?m millipore
} filternotch to grids, 5-15 min {usually} at room temperature), before
} applying the classical UO2Ac-Lead-citrate staining sequence on to the
} ultrathin sections. Then we will have (depending on application time, and
} temperature, respectively) a very discrete to heavily e-dense staining of
} e.g. collagen fibrils, glycogen (+/-"specifically") and some extracellular
} matrix components usually not seen without that pretreatment.
}
} That effect is/will be increased by using a } para-phenylenediamine
} (PPD) {step after osmication (i.e. OsO4 as usual, washing in buffer, EtOH
} 50%, followed by an incubation of tissue samples in 1% PPD in 70%EtOH for
} about 25-30 min-at-rtemp, washing several times unless solution is clear,
} further dehydration-processing steps as usual).
} Additionally, I found that such a treatment generally results in a better
} stabilization of diseased tissue and therefore also better visualisation of
} most tissue components at the ultrastructural level.
}
} I would like to send to you a paper as .pdf you perhaps might not have in
} your library.
} The article does not describe TA } used as a primary fixative in EM {, but,
} instead, tells us about the mechanisms of effects exerted by TA-and
} TA-mimicking dendrimers on to mucosal scaffolds for transplantations, as
} evaluated also by TEM......interesting work.....
}
}
} Best regards and wishes to you and yours,
}
} Wolfgang Muss
} Salzburg/Austria
}
} ----------
} Von: Mike Reedy[SMTP:mike.reedy-at-cellbio.duke.edu]
} Gesendet: Mittwoch, 30. November 2005 16:37
} An: W.Muss-at-salk.at
} Betreff: Re: AW: [Microscopy] RE: Re: SEM imaging of bacteria
} encapsulation
}
} { {Datei: 1991 BJ Abstracts -at- TAURAC, TAOS.pdf} }
}
}
}
}
} Dear Dr Muss,
}
} Thank you for the kind holiday wishes. May yours be also wonderful!
} Here is the PDF, with my hopes you find the method useful.
}
} -mike reedy-
}
}
} } Good morning,
} }
} } dear Dr. Reedy,
} }
} } I greatly should appreciate receiving by e-mail the .pdf on the
} } TAURAC-methods mentioned below (which by no means you will be able to
} } transmit via the Listserver because any attachment will be blocked and
} } deleted).
} }
} } Have a nice and calm "advent-" and a beautiful Christmas time,
} } yours thankfully
} }
} }
} } Wolfgang Muss PhD
} } EM-Lab., PATHOLOGY SALK
} } SALZBURG, Austria
} }
} }
} }
} }
} } ----------
} } Von: mike.reedy-at-cellbio.duke.edu[SMTP:mike.reedy-at-cellbio.duke.edu]
} } Antwort an: mike.reedy-at-cellbio.duke.edu
} } Gesendet: Dienstag, 29. November 2005 23:22
} } An: W.Muss-at-salk.at
} } Betreff: [Microscopy] Re: SEM imaging of bacteria encapsulation
} }
} } ------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html


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16, 21 -- Subject: Re: AW: AW: [Microscopy] RE: Re: SEM imaging of bacteria
16, 21 -- encapsulation; tannic
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From: Elliott-at-Arizona.EDU
Date: Thu, 1 Dec 2005 17:02:58 -0600
Subject: [Microscopy] Re: GFP & Triton X-100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would use an anti-GFP and do the labeling that way. There may be
some GFP left after that treatment, but I would not count on it. GFP
antibodies will work well.
David



On Dec 1, 2005, at 3:23 PM, phillipst-at-missouri.edu wrote:

}
}
}
} ----------------------------------------------------------------------
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} Does anyone know if GFP fluorescence survives when formaldehyde-fixed
} tissues are permeabilized with Triton X-100? I need to double
} label a GFP
} expressing tissue. thanks, tom
}
}
}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 2 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
}
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From: leunissen-at-aurion.nl
Date: Thu, 1 Dec 2005 20:09:35 -0600
Subject: [Microscopy] Re: viaWWW: Immunogold and marine larvae

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Catherine

I'll be happy to help, but could you let me have some more info as to
whether you need help on specimen preparation, or immunolabelling?
There is a basic protocol on our website: http://www.aurion.nl via
Technical Support} } Incubation Protocol that should work with any good
quality gold conjugate.

Cheers

Jan Leunissen

Aurion - President Present Address:
Costerweg 5 EM-Unit
6702 A Wageningen Otago School of Medicine
The Netherlands Dunedin, New Zealand
phone 31-317-497676 phone 64-3-4797109
fax 31-317-415955 fax 64-3-4797254
http://www.aurion.nl http://ocem.otago.ac.nz
------------------------------------------------------------------------
--------
"Light Microscopy? Is that for people on a diet? ". . . Eva Leunissen
(12)


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From: khlee-at-ybust.edu.cn
Date: Thu, 1 Dec 2005 22:01:25 -0600
Subject: [Microscopy] SEM looking for a sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Last week we finished installation of a used JEOL 6100 SEM in our materials
science department. But a scope only. Thus we need to purchase many items.
We will do this one by one as necessary. Right now I am looking for a few
essential equipments such as a sputter coater. Though we prefer to buy a
used one, purchasing a new one can be an option.

So I would like to have your wise advice on purchasing a sputter coater. We
need to do carbon coating and Au-Pd coating. Among mid to low price range,
which brands have good reputations?

I used to use SEM(EBSD) and TEM while studying in U.S. Now I teach materials
science in China. In this area, this is the first SEM installed. So many
(colleges and local government) have interests in our SEM and services.
Thank you very much in advance.

Elliot
++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Elliot K. Lee, Ph.D.
Associate professor
School of Materials, Mechanical & Automation Engineering
Yanji city, Jilin Province
CHINA
(office) +86-433-291-2975
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++


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From: W.Muss-at-salk.at
Date: Fri, 2 Dec 2005 06:13:28 -0600
Subject: [Microscopy] Re: SEM imaging of bacteria encapsulation; tannic acid

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr. Reedy, dear all,

first of all, thank you very much for your kind and informative reply.

I regret to say that for given reasons (see use of MSA Listserver) I did
not respond to the MSA Listserver with an enclosed pdf, instead of, my
reply only I directed to your personal e-mail-address.
For all eventually interested in:
The stated "TA and TAMD dendromer" paper entitled:
Tannic acid mimicking dendrimers as small intestine submucosa
stabilizing nanomordants
by Vladimir Kasyanova, Jason Isenburg, Robert A. Draughn, Starr Hazard,
Jason Hodde, Iveta Ozolanta, Modra Murovska, S. Bart Halkes,
Ioannis Vrasidas, Rob M.J. Liskamp, Roland J. Pieters, Dan Simionescu,
Roger R. Markwald, Vladimir Mironov*)
has been published in
Biomaterials, 27 (2006) 745-751 and there are copyrights attributed to
Elsevier Publishers 2006, so I am not able to provide it on request.

So please, send your request to
*) Corresponding author Tel.: +843792 7630; fax: +843 792 0664.
E-mail address: mironovv-at-musc.edu (V. Mironov).

It is not the right time and place, perhaps, to go into further detail on
all the very interesting facets of the information you gave on the use of
TA in your lab....perhaps we can write offline about that.
I would like to add here for all those interested the original reference
for the ultrathin section pre-staining step (used before
Uranyl-acetate-Lead citrate procedure) we use routinely since 1990 in our
lab with very excellent results:

Rapid Contrasting of Extracellular Elements in Thin Sections
Koert P. Dingernans and Marius A. van den Bergh Weerman
Ultrastructural Pathology. 14/6:519-527, 1990 519-527

We use ultraclean glassware and stirrer, as well as triple distilled aqua
for the solution of 0.05-05% TA (sonicating for 10 min or at least heating
the solution, when stirring, up to approx. 45-50 degrees C) and store the
TA-solution as described in a plastic syringe (25-30 ml) in the dark
(several cautions at the time of filling as well of dispensing...i.e.
filtering any way with a 0.25-0.45?m millipore filternotch) and in our
experience the maximum storage time turns out to be approx. 3 months
(storage at room temperature).
Concerning the TA used (which in our experience is of utmost importance):
since 1985 we use a batch of Tannic Acid of a defined Low Molecular Weight
(approx. 1701, as we were told by the selling company MALLINCKRODT**),
Order No. 1764, Tannic Acid Powder AR) and know that there are a lot of
several } Tannic acid powders { out there which do not meet THAT low
molecular weight......we think that this is a very important clue to good
results.
(Note: **) I do not have any interests in advertising that product or do
have an affiliation with Mallinckrodt....perhaps other sources will have
similar or even better product qualities).

I do have a .pdf version of this article which I scanned by myself for
electronic storage in my "methods library" which is not printed quality but
sufficient for reading the important steps necessary. Since the original
publishing of that article is back now 16 years and as a private subscriber
to } Ultrastructural Pathology { since 1988 I do hope not to hurt
} copyrights {, if I respond to some colleagues requesting that .pdf for
their (solely) personal use.

Best wishes and have all a beautiful "advent" and festive christmas time,

yours sincerely
Wolfgang Muss
SALZBURG, Austria
(http://city.salzburg.com , German
http://www2.salzburg.info// English)


----------
Von: Mike Reedy[SMTP:mike.reedy-at-cellbio.duke.edu]
Gesendet: Donnerstag, 01. Dezember 2005 17:55
An: W.Muss-at-salk.at
Cc: Microscopy Listserver
Betreff: Re: AW: AW: [Microscopy] RE: Re: SEM imaging of bacteria
encapsulation; tannic

Dr Muss-
Thanks for the paper and information about your uses of TA.

(The inserted PDF attachment you sent came to me
through the list-server. How did that succeed?
I will try here to to insert my PDF of the 1991
abstract (see below) and see if it passes to
become available to other readers.)

I have been meaning for some time to try TA as a
first-step section stain, as suggested by some
papers of ~15 years ago (At the moment i can
track down
Haldar et al (J Clin Pathol, (1992),
45:633-635; use a MIX of TA + UrAc!) and
Stirling (J.Histochem.Cytochem. (1993) 41:643-648.)
Maybe I will finally do so, now that I know your procedure.

I'm interested that you keep it in the dark as a
prepared solution. When we started using TA with
GA in early 1979 (inspired by David Begg et al
paper in
J Cell Biol (1978) 79:846-852
we soon got the idea that we could not gain the
benefits if improved contrast and better
preservation if we stored TA as a prepared or
stock solution. The color changed a bit after
1-2 days, became darker I think, and the
structural preservation was not so nice. Ever
since, we make it a habit to dissolve TA fresh,
whether using it in a mix with GA or simply alone
as primary fix, starting the fix no later than
about 3 hours after dissolving the TA, same
precaution whether in aqueous or
cryo-substitution-acetone procedures. Perhaps
keeping it in the dark might prolong its useful
life in solution, but because we lost 2+ weeks of
work back in 1979 before we learned our lesson,
we chose after that not to explore the use of
aged TA solutions.

The TA and TAMD dendromer paper you sent is
interesting. We are at the moment more
interested in low low MW mimics of TA like
catechin, because even the best low MW TA seems
unable to penetrate and carry stain mordanting
properties to the interior of globular protein
domains-- even if the UrAc or OsO4 is applied
before the TA it does not attract the TA to the
interior of myofilaments. The result is some
degree of negative staining on the smallest
scale; it appears to produce a dense nanoshell
around actin monomers and myosin head globular
domain in our most detailed EM tomography of thin
sections from cryo-substituted insect flight
muscle fibers
(J Struct Biol. (2004) 147:268-282).
(We have several more detailed and convincing
density-contoured images than the published
figure.)
This shell remains more dens than any interior
positive staining that may also occur, despite
the very intense section staining produced by
the permanganate -} Sato's Pb stain sequence we
use. We suppose that TA is probably ALWAYS
producing a surface coat on globular protein
domains, regardless of whether it also may
penetrate. Using catechin instead sometimes
seemsed to make the staining more positive (by
eye, not tomography) in some regions of sections
from a muscle fiber so treated. TA may produce
or allow more permeating and homogeneous staining
of very slender 2-4 nm molecular strands like the
lever-arm of the myosin heads, and even of much
fatter structures, such as the M-region of thick
filaments which often appear a glassy uniform
gray despite the nano-negative staining of the
adjacent thin filaments and the A-band.

All of this is to say why we are not immediately
interested in trying the TA dendromers, and why
we need to try TA (and probably also catehin) in
a section staining procedure. Perhaps at the cut
surface of a section it can penetrate internal
regions of actin monomers and myosin heads and
filament backbones that proved rather
impenetrable when exposed to TA in liquid
fixatives.

-mike reedy-

you must double-click on the apparently blank pdf
just below to make it open in Acrobat and show
its content.



}
}
}
} Dear Dr. Reedy,
}
} I would like to thank you very much for your copy "service".
} These abstracts I gladly shall integrate into my "library of EM-methods".
}
} I found TA (if "low molecular weight") very helpful in stabilizing
} structures and demonstrating structure usually not "included" in stained
} sections if processed the "normal" schedule (FA-GA,GA,OsO4,Dehydration).
}
} Also we (in a diagnostic EM-Lab) use this stuff within a pre-incubation
} step (0.05-0.5% w/v hydrous solution, sonicated, filtered and stored in
} plastic syringes in the dark, dispensed via a 0.25 or 0.42 ?m millipore
} filternotch to grids, 5-15 min {usually} at room temperature), before
} applying the classical UO2Ac-Lead-citrate staining sequence on to the
} ultrathin sections. Then we will have (depending on application time, and
} temperature, respectively) a very discrete to heavily e-dense staining of
} e.g. collagen fibrils, glycogen (+/-"specifically") and some extracellular
} matrix components usually not seen without that pretreatment.
}
} That effect is/will be increased by using a } para-phenylenediamine
} (PPD) {step after osmication (i.e. OsO4 as usual, washing in buffer, EtOH
} 50%, followed by an incubation of tissue samples in 1% PPD in 70%EtOH for
} about 25-30 min-at-rtemp, washing several times unless solution is clear,
} further dehydration-processing steps as usual).
} Additionally, I found that such a treatment generally results in a better
} stabilization of diseased tissue and therefore also better visualisation
of
} most tissue components at the ultrastructural level.
}
} I would like to send to you a paper as .pdf you perhaps might not have in
} your library.
} The article does not describe TA } used as a primary fixative in EM {, but,
} instead, tells us about the mechanisms of effects exerted by TA-and
} TA-mimicking dendrimers on to mucosal scaffolds for transplantations, as
} evaluated also by TEM......interesting work.....
}
}
} Best regards and wishes to you and yours,
}
} Wolfgang Muss
} Salzburg/Austria
}
} ----------
} Von: Mike Reedy[SMTP:mike.reedy-at-cellbio.duke.edu]
} Gesendet: Mittwoch, 30. November 2005 16:37
} An: W.Muss-at-salk.at
} Betreff: Re: AW: [Microscopy] RE: Re: SEM imaging of bacteria
} encapsulation
}
} { {Datei: 1991 BJ Abstracts -at- TAURAC, TAOS.pdf} }
}
}
}
}
} Dear Dr Muss,
}
} Thank you for the kind holiday wishes. May yours be also wonderful!
} Here is the PDF, with my hopes you find the method useful.
}
} -mike reedy-
}
}
} } Good morning,
} }
} } dear Dr. Reedy,
} }
} } I greatly should appreciate receiving by e-mail the .pdf on the
} } TAURAC-methods mentioned below (which by no means you will be able to
} } transmit via the Listserver because any attachment will be blocked and
} } deleted).
} }
} } Have a nice and calm "advent-" and a beautiful Christmas time,
} } yours thankfully
} }
} }
} } Wolfgang Muss PhD
} } EM-Lab., PATHOLOGY SALK
} } SALZBURG, Austria
} }
} }
} }
} }
} } ----------
} } Von: mike.reedy-at-cellbio.duke.edu[SMTP:mike.reedy-at-cellbio.duke.edu]
} } Antwort an: mike.reedy-at-cellbio.duke.edu
} } Gesendet: Dienstag, 29. November 2005 23:22
} } An: W.Muss-at-salk.at
} } Betreff: [Microscopy] Re: SEM imaging of bacteria encapsulation
} }
} } ------------------------------------------------------------------------
} } ----
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America


--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html


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From: jjf-at-pgt.com
Date: Fri, 2 Dec 2005 06:33:39 -0600
Subject: [Microscopy] SEM Calibration Standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For anyone interested in a calibration procedure, you can refer to ASTM E 766
(2003), "Standard Practice for Calibrating the Magnification of a Scanning
Electron Microscope."

This procedure allows for the use of any suitable physical standard.

John Friel


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From: smalinskas-at-yahoo.com
Date: Fri, 2 Dec 2005 07:56:41 -0600
Subject: [Microscopy] Re: SEM looking for a sputter coater

Contents Retrieved from Microscopy Listserver Archives
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Dr. Lee:

X-from my limited experience with sputter coaters, I
find that the Hummer brand is troublesome. The Denton
brand seems to be favored among my colleagues.

Stu Smalinskas, P.E.
Metallurgist
SKF USA
Plymouth, Michigan
(734) 414-6862

--- khlee-at-ybust.edu.cn wrote:

}
} Hi,
}
} Last week we finished installation of a used JEOL
} 6100 SEM in our materials
} science department. But a scope only. Thus we need
} to purchase many items.
} We will do this one by one as necessary. Right now I
} am looking for a few
} essential equipments such as a sputter coater.
} Though we prefer to buy a
} used one, purchasing a new one can be an option.
}
} So I would like to have your wise advice on
} purchasing a sputter coater. We
} need to do carbon coating and Au-Pd coating. Among
} mid to low price range,
} which brands have good reputations?
}
} I used to use SEM(EBSD) and TEM while studying in
} U.S. Now I teach materials
} science in China. In this area, this is the first
} SEM installed. So many
} (colleges and local government) have interests in
} our SEM and services.
} Thank you very much in advance.
}
} Elliot
}
++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} Elliot K. Lee, Ph.D.
} Associate professor
} School of Materials, Mechanical & Automation
} Engineering
} Yanji city, Jilin Province
} CHINA
} (office) +86-433-291-2975
}
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++
}



__________________________________
Start your day with Yahoo! - Make it your home page!
http://www.yahoo.com/r/hs

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From: m_mo_shad-at-yahoo.com
Date: Fri, 2 Dec 2005 08:31:21 -0600
Subject: [Microscopy] viaWWW: casein micelles

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This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
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Email: m_mo_shad-at-yahoo.com
Name: Mary Shad

Title-Subject: [Filtered] casein micelles

Question: why should we use SEM and TEM both togather specially for studing casein micelles in milk?

what is the best way for determination the size of casein micelles by using elctron microscopy?

And what is the best way for sample preparation of casein micelles that we have a little change in casein micelle size?

---------------------------------------------------------------------------

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From: jafarhan-at-rci.rutgers.edu
Date: Fri, 2 Dec 2005 09:16:15 -0600
Subject: [Microscopy] Atomic positions for WO2.9

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Dear All,

I am doing some research with Tungsten Oxide. I got WO2.9 phase which is
tetragonal phase with space group of P4/nmm (from XRD). I wonder if anybody
have the atomic positions and Waykoff notations for this phase.

Thank You

Jafar

Rutgers University


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From: gerd.leitinger-at-meduni-graz.at
Date: Fri, 2 Dec 2005 09:55:18 -0600
Subject: [Microscopy] EM: LR White contrast

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Does anyone have experience in contrasting LR white embedded thin
sections with uranyl acetate and lead citrate?

I am new to using LR white, and tried halving the "normal" incubation
times in uranyl acetate and lead citrate, but the sections are unusable-
much too dark and full of stain deposits.

Can anybody with experience with LR White suggest a staining
protocol?

thank you.



Dr. Gerd Leitinger

Institut für Zellbiologie, Histologie und Embryologie
Medizinische Universität Graz
Harrachgasse 21
A-8010 Graz
Austria

Tel. ++43 316 380 4237
Fax. ++43 316 380 9625
Mailto: Gerd.Leitinger-at-meduni-graz.at



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From: lcgould-at-med.cornell.edu
Date: Fri, 2 Dec 2005 11:02:06 -0600
Subject: [Microscopy] Re: EM: LR White contrast

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Gerd,
I contrast LR White thin sections with uranyl acetate (3% aqueous)
for 5 minutes followed by 3 minutes in Pb citrate (Venable &
Coggeshall). Wash thoroughly with water after the UA and with 0.01N
NaOH followed by water after the lead.
I have found this to give good contrast, and I can still see the gold
label easily.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org

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From: dsoren-at-umich.edu
Date: Fri, 2 Dec 2005 15:08:54 -0600
Subject: [Microscopy] diamond knives resharpening

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Hello listers,

We are trying to decide whether to buy new or to have resharpened a
couple of Diatome diamond knives. Has anyone had any experience with
the Diatome resharpened knives? Are they as good as new? What was
the turn-around time for resharpening?

Thanks for your comments,

Dotty Sorenson

Dorothy Sorenson
Microscopy and Image-analysis Laboratory
Department of Cell and Developmental Biology
University Of Michigan Medical School
4643 Medical Science Building II
1301 Catherine
Ann Arbor, MI 48109-0616
(734)763-1170
FAX (734)763-1166


==============================Original Headers==============================
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From: Elliott-at-Arizona.edu
Date: Fri, 2 Dec 2005 15:25:01 -0600
Subject: [Microscopy] Re: diamond knives resharpening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My understanding is that unless you specifically request re-sharpening,
what you get is a new knife that is about the same size as what you
started with. New diamond and new boat. It's well worth the money.
David


On Dec 2, 2005, at 2:13 PM, dsoren-at-umich.edu wrote:

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} Hello listers,
}
} We are trying to decide whether to buy new or to have resharpened a
} couple of Diatome diamond knives. Has anyone had any experience with
} the Diatome resharpened knives? Are they as good as new? What was
} the turn-around time for resharpening?
}
} Thanks for your comments,
}
} Dotty Sorenson
}
} Dorothy Sorenson
} Microscopy and Image-analysis Laboratory
} Department of Cell and Developmental Biology
} University Of Michigan Medical School
} 4643 Medical Science Building II
} 1301 Catherine
} Ann Arbor, MI 48109-0616
} (734)763-1170
} FAX (734)763-1166
}
}
} ==============================Original
} Headers==============================
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==============================Original Headers==============================
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From: nairvinods-at-gmail.com
Date: Fri, 2 Dec 2005 17:12:15 -0600
Subject: [Microscopy] EM: LR White contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gerd,
I have been using LR white embedded animal tissue for my research.
After having immuno labelled the grids, I stain them with 5% UA for 10
mins follwed by a 1 min rinse in distilled water. I use few pellets of
NaOH in a covered petridish containing lead citrate drops and incubate
the grids in lead citrate for 10 mins. Thereafter a quick rinse for 1
min in distilled water and view the grids after they have been
airdried for about 5 mins.
regards,
Vinod
Grad Student,
Dept. Of Biology
New Mexico State University,

On 12/2/05, lcgould-at-med.cornell.edu {lcgould-at-med.cornell.edu} wrote:
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} Gerd,
} I contrast LR White thin sections with uranyl acetate (3% aqueous)
} for 5 minutes followed by 3 minutes in Pb citrate (Venable &
} Coggeshall). Wash thoroughly with water after the UA and with 0.01N
} NaOH followed by water after the lead.
} I have found this to give good contrast, and I can still see the gold
} label easily.
} Lee
} --
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy & Histology Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
} http://www.cornellcelldevbiology.org
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From: lgiannuzzi-at-adelphia.net
Date: Fri, 2 Dec 2005 17:20:33 -0600
Subject: [Microscopy]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Senior Applications Engineer - Dual Beam Technologies (FIB, SEM)


FEI Company (www.feicompany.com) is a leading supplier of "tools for
Nanotechnology". We develop and manufacture tools that enable research,
development and manufacture of Nanoscale features by helping our customers
understand their three-dimensional structures. We serve the semiconductor,
data storage, structural biology and industrial markets, where decreasing
feature sizes drive the need for our structural process technology
solutions. Our solutions are based on a combination of patented and
proprietary technologies that provide industry-leading capabilities to view,
measure, analyze, and modify physical structures at atomic, or nanometer
scale resolutions. Simply put, FEI helps the world of nanotechnology develop
products faster, control manufacturing processes better, and understand the
structures of complex substances.


Job Location: Oregon, USA

Job Responsibilities:

The Senior Applications Engineer in the Dual Beam Group is responsible for
providing technical expertise in the support of FEI's North American sales
efforts. Primary responsibilities include the following:


* Performing high quality dual beam product demonstrations, including
the development of new techniques and applications for FEI partners and
customers.
* Cultivating positive customer relationships and acting as a high
level customer interface for specific instrument/applications issues, acting
as a technical expert, explaining complex technology details, giving in
depth presentations, and assisting in technique development.
* Supporting and training customers after the sale has been made and
the system is signed off. Supporting Service Engineers on instrument sign
offs and specific application techniques.
* Providing continuous feedback to the product groups on system
performance, features, and problems while collaborating with
marketing/development groups on future product developments.


Position Requirements -

This position is ideal for an experienced technologist wanting to be
involved with a dynamic team and exposed to a constant variety of customer
application areas. The successful candidate will possess the following
combination of education and experience:


* At a minimum, a BS degree in materials, physics or chemical
engineering. Higher degree and advanced knowledge about electrical
engineering and/or Semiconductor Fabrication is greatly preferred.
* 3 or more years experience in a relevant commercial/research
establishment with recent experience utilizing Focused Ion Beam (FIB) and
Scanning Electron Microscopy (SEM) technology in sample preparation,
preferably across a variety of application areas, i.e., nanoelectronics,
materials, thin films, etc. Additional experience in the areas of SEM/EDS,
STEM and TEM is greatly preferred.
* Working knowledge and confidence with PC platforms, scripting,
Windows 2000 and PowerPoint desirable.
* Ability to write technical papers, technical application reports and
training manuals
* Excellent, enthusiastic, clear communication skills with a diverse
audience in a commercial sales focused environment is critical to the
success of this position.
* Ability to travel both domestically as well as internationally and
possession of a valid passport.



Please send your resume to: Trish Rice, trice-at-feico.com


==============================Original Headers==============================
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From: gary-at-gaugler.com
Date: Fri, 2 Dec 2005 22:17:15 -0600
Subject: [Microscopy] =?iso-8859-1?Q?Re:__Quantomix=AE_sample_holders?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

IMO, the Quantomix units are very nice but horribly
expensive. If SPI has an equivalent but lower cost
alternative, this is good.

The basic idea/concept is quite novel. I saw this at
M&M 2005 and liked it. But the cost was a big detractor.

gary g.



At 10:14 AM 11/27/2005, you wrote:



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From: gary-at-gaugler.com
Date: Sat, 3 Dec 2005 00:09:25 -0600
Subject: [Microscopy] SEM looking for a sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I echo this. I replaced two sequential Hummers with
a new Denton Desk IV TSC. For ultra fine coating, the
TSC turbo is essential. To absolutely get rid of hydrocarbon
contamination, I had the standard oil diaphram pump replaced with
an Edwards XDS5 dry scroll pump. It takes a little longer to
coat from start to finish but the results are
stunning.


The SEM is totally dry pumped.

Having said this, you cannot expect that your very special specimen
will always be hydrocarbon-free. Being exposed to atmosphere for
not all that long of time will compromise it. So get a dessicator
or vacuum container to hold the specimens after coating and use.
You WILL see a difference if not. The weak link in the chain is the
oil mech pump for the dessicator unit. This can be solved by using
an anti-backstreaming trap, dessicator chamber and other measures.


The key is keep oil and atmosphere away from the specimen. Otherwise use
1-3KV and deal with it. Wide field first, high mag last.

gary g.



At 05:58 AM 12/2/2005, you wrote:



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==============================Original Headers==============================
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From: terry-at-AsylumResearch.com
Date: Monday, Dec. 12
Subject: [Microscopy] Atomic Force Microscopy Tutorial at ASCB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Asylum Research will present "How to Choose an Atomic Force
Microscope for Biological Research", tutorial at the American Society
for Cell Biology Conference (ASCB).

Place: Moscone Convention Center, San Francisco

Additional information can be found at http://www.asylumresearch.com/
News/Events.shtml.

Regards,
Terry Mehr
Asylum Research
www.AsylumResearch.com


==============================Original Headers==============================
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From: dsoren-at-umich.edu
Date: Mon, 5 Dec 2005 14:34:41 -0600
Subject: [Microscopy] Diamond knives

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Many thanks to all those who responded to my question about new vs
resharpened diamond knives. The overwhelming consensus is that
resharpened are as good as new and that the turn-around time for
resharpening is around 4 weeks.

Dotty

Dorothy Sorenson
Microscopy and Image-analysis Laboratory
Department of Cell and Developmental Biology
University Of Michigan Medical School
4643 Medical Science Building II
1301 Catherine
Ann Arbor, MI 48109-0616
(734)763-1170
FAX (734)763-1166


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From: jontes.1-at-osu.edu
Date: Mon, 5 Dec 2005 17:51:39 -0600
Subject: [Microscopy] viaWWW: RMC and PoweTomer X

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Email: jontes.1-at-osu.edu
Name: James Jontes

Organization: The Ohio State University

Title-Subject: [Filtered] RMC PoweTome X

Question: Hi,
I am planning to buy a routine, room temperature ultramicrotome and am considering the UC6 or the PowerTome X. I don't know that much about RMC, but there is a $15,000 price differential. Is there anything wrong with the RMC? Does anyone have any horror stories?

There was a similar question a while ago, but I couldn't determine the answer from the posts.

Thanks very much for your help.



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From: lcgould-at-med.cornell.edu
Date: Tue, 6 Dec 2005 08:14:41 -0600
Subject: [Microscopy] Re: viaWWW: RMC and PoweTomer X

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James,
I have a (now old) RMC7000 and a (also old) Leica Ultracut S. They
both have their strengths and weaknesses. Both have needed repairs
at one time or another. I have the RMC under a service contract, so
its repairs were painless, in terms of cost. I inherited the Leica
(I'm actually its 3rd home) and I have had a local service group give
it a check up once in a while, and they replaced the mother board in
the control unit when it literally went up in smoke earlier this
year...very dramatic and not an inexpensive repair.
The local service people I use are also the regional reps for the
Leica ultra'tomes, so service is usually very quick (as fast as I can
get a PO cut..faster if I use a credit card). RMC as a regional
service person for the North East. He comes in for the annual PM and
if I need service.
In terms of performance, the 2 are comparable in my hands.
I don't know how the newest models from each company compare to one
another. Aside from cost (not an insubstantial consideration, and
the reason I have the RMC in the first place), I would try to get
some hands-on time on each for you or the person who will be using it
most, and get a real sense for the ergonomics, etc. This comment
comes from someone with 2 degenerating cervical disks....probably
exacerbated by 25+ years of sitting at microtomes and microscopes of
various types.
I hope this is helpful,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org

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From: paulrc-at-bilbo.bio.purdue.edu
Date: Tue, 6 Dec 2005 08:43:46 -0600
Subject: [Microscopy] open position

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Dear List,

A vacancy for a microscopist in the Structural Virology Group of
Purdue University is anticipated. This position is ideal for an
individual interested in cryo-electron microscopy studies of viruses.
The position will involve sample preparation, initial sample
assessment, sample freezing, data collection, image analysis,
three-dimensional modeling and interpretation. The ideal candidate
will be expected to be involved in all aspects of the research
including publishing. A BS with a major in biochemistry or a related
area of biology and experience with transmission electron microscopy
is considered a minimal requirement. Additional backgrounds in
physics and computing along with a willingness to learn and the
ability to balance multiple projects would be highly desirable.
Employment will entail comprehensive
training during the first year and extensive daily interactions with
a team of graduate students, post-doctoral scholars and faculty. All
levels of experience will be considered. Please contact Paul Chipman
(765-494-1487, paulrc-at-purdue.edu) for further details.

Purdue University is located in West Lafayette, approximately 70
miles north of Indianapolis and 120 miles south of Chicago. As the
major employer in the region with more than 10,000 faculty and staff,
Purdue is also home to more than 38,000 students and extends a true
"university town" feel to the twin cities of Lafayette and West
Lafayette.

Thanks,
Paul

Paul Chipman
Director, Electron Microscopy Facility
Project Leader, Structural Virology EM Studies
Dept. of Biology, Purdue University
Lilly Hall, Rm. B216
Phone: 765-494-1487
Fax: 765-496-1189


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From: bfoster-at-mme1.com
Date: Tue, 6 Dec 2005 10:03:50 -0600
Subject: [Microscopy] Re: viaWWW: casein micelles

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Dear Mary

Have you thought of using AFM in Phase mode instead? I think you would find that it would get around a lot of the sample prep issues and be much more direct.

Hope this is helpful,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com

P. S.
Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.




At 08:33 AM 12/2/2005, m_mo_shad-at-yahoo.com wrote:



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From: mike.reedy-at-cellbio.duke.edu
Date: Tue, 6 Dec 2005 13:35:36 -0600
Subject: [Microscopy] Re: EM: LR White contrast

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Gerd-
You might be interested In several papers by
S.-H. Brorson in the journal Micron, most from
the 1990s, all electronically retrievable as
PDFs. I recently became interested in these
because of some unusual insights (I have never
seen these grouped together anywhere else!) that
Brorson develops about how raising the content of
accelerator 2-8% or of propylene oxide 0-10% can
reduce crosslink density of the final cured
epoxy, and the relationship of this to
rubbery-versus-brittle macro properties, polymer
bonding to side-chains of embedded biomolecules,
ultramicrotomy cutting quality, post-embedding
antigen exposure, and comparison of
immuno-labeling with LR White.

He reports that reduced x-linking enhanced
"antigen retrieval" (= antigen exposure to
post-embedding immunolabels by incubation of
sections in hot citrate ) in thin epoxy sections,
such that immuno-labeling for larger antigens
could become as efficient as in LR White
(although he notes less or little success with
smaller-smallest antigens).

Micron 29:89-95 (1998) is one of these. However,
Micron 35:619-621 (2004) seems to hint that he
may have given up the high-accelerator (8%)
approach in favor of etching with citrate at
95-145 degrees C and immunostaining at 60°C. I
can't tell for sure, so I will copy this message
to Brorson himself, hoping for his answer.

You might also be interested in the obscurely
published finding (Web of Science does not seem
able retrieve it so i can't detect who might have
cited it) that quite good structure and antigen
survival can be achieved after using uranyl
acetate as a primary fixative. (Fassel &
Greaser, 1997, Microsc. Res Tech 37:600-601).

-mike reedy-

At 9:58 AM -0600 12/2/05, gerd.leitinger-at-meduni-graz.at wrote:
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--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html


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From: matthems-at-rose-hulman.edu
Date: Tue, 6 Dec 2005 15:34:15 -0600
Subject: [Microscopy] viaWWW: refractive index of mitochondria,nucleus

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Email: matthems-at-rose-hulman.edu
Name: ann

Organization: rose hulman institute of technology

Title-Subject: [Filtered] Required Data

Question: HI Everyone,I am doing a reserach assignment for which i require the values of teh refractive index of mitochondria,nucleus,ribosome,cytoskeleton at various wavelengths.I have tried searching for these values,but have got unsatisfactory results.If anyone knows where i can get such data,could anyone plz help.
Thanx

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From: mike.reedy-at-cellbio.duke.edu
Date: Tue, 6 Dec 2005 19:51:00 -0600
Subject: [Microscopy] Re: viaWWW: refractive index of mitochondria,nucleus

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Matt,
Most biological macromolecules, whether Lipid, CHO, NA or protein,
have a specific refractive increment very near .0018, meaning that
each 1.0 g of dry weight dissolved (or suspended) in 100 g of aqueous
solution increases the averaged refractive index of that solution or
of that volume occupied by the molecular assembly by .0018. Thus if
water RI is 1.3300, 1% protein (w/w) is 1.3318. Dry 100% protein
would be about 1.58 (1.33 + .185) if specific RI increment is .00185
(behavior is not reliably this linear though at high conc'ns). I
learned about all this while doing interference microscopy of
myofibrils about 25 year ago. In our lab we still have a usable
Vickers M86 scanning microinterferometer microscope that the last
user coupled nicely to a Macintosh program, but we haven't found a
fundable use for it in the last 20 years.

See Barer and Joseph, Quart J Microsc Sci (1954) 95:399-423, for a
discussion of refractometry of living cells and info about the
specific refractive increments of solutions of the different
macromolecules, varying in reality from perhaps.0014 to .0020. For
more references of possible interest, including parts 2 and 3 of
Barer and Joseph (1954-55), see the reference list in Joseph, 1981,
J. Microscopy, 131:163-172. I'm not sure anyone ever measured
isolated organelles except for myofibrils,. You can do it yourself
using a phase contrast microscope and irrigating under the coverslip
with impermeant immersion medium of graded dilutions to give graded
steps in RI, in order to see which RI matches out the contrast of the
isolated organelle of interest, making them almost phase-invisible.
The two media I used for matching A-bands in myofibrils of insect
flight muscle and rabbit psoas were Percoll, and Limulus hemocyanin,
washed and finally concentrated by ultracentrifugation in the buffer
of choice, then incrementally diluted and subject to RI measurementss
in an Abbe refractometer (M. K. Reedy, C. Lucaveche, Adv Exp Med Biol
170:29-45 (1984)). Percoll was more innccuous than hemocyanin on
rabbit fibrils; insect fibrils were unchanged by either one.

-mike reedy-

At 3:40 PM -0600 12/6/05, matthems-at-rose-hulman.edu wrote:
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--
-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
Dept. Cell Biology, Box 3011 (for U.S. Mail)
458 Alex Sands Bldg, Research Dr (courier)
Durham, NC 27710

Office 919-668-2534
Lab 919-684-5674
Fax 919-681-9929
mike.reedy-at-cellbio.duke.edu
http://note.cellbio.duke.edu/Faculty/Research/Reedy.html

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From: mike.reedy-at-cellbio.duke.edu
Date: Tue, 6 Dec 2005 20:10:45 -0600
Subject: [Microscopy] Re: viaWWW: refractive index of mitochondria,nucleus

Contents Retrieved from Microscopy Listserver Archives
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Ann,
One more reference ; in a 1966 book Barer wrote a more
reader-friendly text on the method that might still give you what you
want; I found it a pretty good read. The reference is
1. R. Barer, in Physical Techniques in Biological Research A. W.
Pollister, Ed. (Academic Press, New York., 1966) pp. 1-56.

-mike reedy-

At 3:40 PM -0600 12/6/05, matthems-at-rose-hulman.edu wrote:
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-mike reedy-

************************
Michael K. Reedy, M.D.
Duke Univ. Med. Center
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From: aarti_harle-at-yahoo.co.in
Date: Wed, 7 Dec 2005 01:41:05 -0600
Subject: [Microscopy] AFM sample prepartion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello

We seek help for the sample prepartion for AFM
ananlysis.
We are trying to scan the suspended nanoparticles in
semicontact mode. Basically We made a smear on glass
slide and observed but unable to obtain the good
pictures.

Thanks in advance

Regards
Shrunali Kulkarni, Scientist
Institute of Microbial Technology
India



__________________________________________
Yahoo! DSL – Something to write home about.
Just $16.99/mo. or less.
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From: tttan-at-simtech.a-star.edu.sg
Date: Wed, 7 Dec 2005 07:51:36 -0600
Subject: [Microscopy] viaWWW: Custom Electron Gun developer

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Email: tttan-at-simtech.a-star.edu.sg
Name: Tan T. T.

Organization: SIMTech

Title-Subject: [Filtered] Custom Electron Gun developer

Question: Dear all,

I would like to know if anyone has contacts for custom electron gun development.

I need a 120kV field emission electron gun, no scan coil etc. Just the gun alone, with certain specifications.

Would appreciate if you could forward me the names of the companies that do this.

Thank you very much.

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: cheng.huang-at-anu.edu.au
Date: Wed, 7 Dec 2005 07:52:38 -0600
Subject: [Microscopy] viaWWW: Hard drive for the Link EDS system

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Email: cheng.huang-at-anu.edu.au
Name: Cheng

Organization: CSIRO

Title-Subject: [Filtered] Hard drive in the Link system

Question: Hi,

The hard drive of our old Link x-ray analysis system was crashed recently. We are looking for the replacement. Does anyone by any chance still have this system or hard drive (40 or 80 MB, SCSI) stashed somewhere and won't need anymore? We are happy to pay for it.

Thanks,

Cheng

PI Industry
CSIRO
Australia


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From: ab78-at-esc.cam.ac.uk
Date: Wed, 7 Dec 2005 08:18:33 -0600
Subject: [Microscopy] Re: viaWWW: Hard drive for the Link EDS system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A few years ago I bought an obselete HD for a Link from

http://www.dolphinhitec.co.uk/Sussex/Dolphin%20Hitec/harddrives.htm

They still have one or 2 of the models I bought listed on their webpage
- though they are in the UK.




cheng.huang-at-anu.edu.au wrote:
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} Email: cheng.huang-at-anu.edu.au
} Name: Cheng
}
} Organization: CSIRO
}
} Title-Subject: [Filtered] Hard drive in the Link system
}
} Question: Hi,
}
} The hard drive of our old Link x-ray analysis system was crashed recently. We are looking for the replacement. Does anyone by any chance still have this system or hard drive (40 or 80 MB, SCSI) stashed somewhere and won't need anymore? We are happy to pay for it.
}
} Thanks,
}
} Cheng
}
} PI Industry
} CSIRO
} Australia
}
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 11, 12 -- From zaluzec-at-microscopy.com Wed Dec 7 07:52:38 2005
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--

Andy Buckley

AB78-at-ESC.CAM.AC.UK
DEPARTMENT OF EARTH SCIENCES
UNIVERSITY OF CAMBRIDGE Tel +44 1223 333469
DOWNING STREET +44 1223 333400
CAMBRIDGE FAX +44 1223 333450
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From: mmcheath-at-mailbox.syr.edu
Date: Wed, 7 Dec 2005 10:09:46 -0600
Subject: [Microscopy] uProbe: WDS detectors - availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I don't have anything that small anymore. You might check around for
some old Macintosh computers and see if you can find a suitable drive.
The nice thing about SCSI is that it was a standard. The only rub might
be matching up the connector, but I think most drives used the 50-pin
rectangular connectors back in that day.

Good luck.
Warren Straszheim

-----Original Message-----
X-from: cheng.huang-at-anu.edu.au [mailto:cheng.huang-at-anu.edu.au]
Sent: Wednesday, December 07, 2005 7:53 AM
To: wesaia-at-iastate.edu

Hi,
I am looking to acquire one or two WDS spectrometers that fit
on any of these JEOL instruments: 733, 840, 6300, 6400, and the 8600.
If you have any in your lab that you are not using I'd be very
interested to hear from you!

Cheers
Mike

--
********************************************************************
Michael M. Cheatham
312 Heroy Geology Laboratory Phone (315)-443-1261
Syracuse University Fax (315)-443-3363
Syracuse, NY 13244-1070

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From: sstan33-at-yahoo.com
Date: Wed, 7 Dec 2005 10:10:20 -0600
Subject: [Microscopy] Re: AFM sample prepartion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Shrunali,

Spin-coating would be helpful. I used to spin coat
magnetic nanoparticles onto newly cleaved mica
substrate. The results were amazing; there almost were
not big aggregates on the substrate.

Alternatively, you may try direct deposition of your
suspension on the substrate. However, in this case you
have to remove excess solvent by rinsing it with the
solvent used in your sample preparation. I
successfully got individually distributed magnetic
nanoparticles on mica, too.

Wish this helps,

Susheng
Department of Chemistry
Oklahoma State University
Stillwater, OK 74075

--- aarti_harle-at-yahoo.co.in wrote:

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}
} We seek help for the sample prepartion for AFM
} ananlysis.
} We are trying to scan the suspended nanoparticles in
} semicontact mode. Basically We made a smear on glass
} slide and observed but unable to obtain the good
} pictures.
}
} Thanks in advance
}
} Regards
} Shrunali Kulkarni, Scientist
} Institute of Microbial Technology
} India
}
}
}
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From: michael-at-Shaffer.net
Date: Wed, 7 Dec 2005 10:50:02 -0600
Subject: [Microscopy] PVA adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

In the past I have used PVA dissolved in EtOH as an adhesive. (i.e., spread
the liquid on a surface, and let dry ... which would leave a very thin film
that would become tacky when heated).

That was then ... and now I'm not sure what "PVA" is. I thought it was
polyvinyl alcohol, but it may be polyvinyl acetate. If anyone knows, please
remind me ... and provide a source or manufacturer if possible. (I also
note that Sigma-Aldrich lists 3 different PV acetates, differeng in mol.wt.)

genuinely :o)
michael shaffer

SEM/MLA Laboratory Coordinator
(709) 737-6799 (ofc)
(709) 737-6790 (lab)
(709) 737-6193 (FAX)
{http://www.mun.ca/creait/maf/}
{http://www.esd.mun.ca/epma/}

Inco Centre
c/o Memorial University
230 Elizabeth Avenue
P.O. Box 4200
St. John's, NL A1C 5S7




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From: jtwilley-at-sprynet.com
Date: Wed, 7 Dec 2005 12:38:39 -0600
Subject: [Microscopy] PVA adhesive

Contents Retrieved from Microscopy Listserver Archives
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Michael,

PVA stands for polyvinylacetate. PVAH is usually used to distinguish polyvinylalcohol although people tend to be lax on the terminology. The acetate my be hydrolyzed to produce the alcohol. Commercial products often contain partially hydrolyzed versions in order to tailor their properties to a particular use, so both species may be present. If you look at the various PVA and PVAH spectra in an infrared database, you will often find them arranged both by molecular weight and by degree of hydrolysis, that is, by the proportion of esterified to hydroxylated units. As you would expect, the intensities of the hydroxyl and ester carbonyl peaks vary tremendously from 100% PVA to 100% PVAH. The greater the PVAH content, the less the polar organic solvents will dissolve it and the more readily it will dissolve in hydrogen-bonding solvents like alcohols. The common form of "white glue" is an aqueous dispersion of PVA colloids rather than a true solution and when dried cannot be readily redispersed. Unless the molecular weight is small, most PVAs are not soluble in large amounts in any single solvent. Methyl ethyl ketone tends to work best. If you have used ethanol in the past it sounds like the material that you were working with may have been largely PVAH.

John Twilley

-----Original Message-----
X-from: michael-at-Shaffer.net
Sent: Dec 7, 2005 11:50 AM
To: jtwilley-at-sprynet.com

In the past I have used PVA dissolved in EtOH as an adhesive. (i.e., spread
the liquid on a surface, and let dry ... which would leave a very thin film
that would become tacky when heated).

That was then ... and now I'm not sure what "PVA" is. I thought it was
polyvinyl alcohol, but it may be polyvinyl acetate. If anyone knows, please
remind me ... and provide a source or manufacturer if possible. (I also
note that Sigma-Aldrich lists 3 different PV acetates, differeng in mol.wt.)

genuinely :o)
michael shaffer

SEM/MLA Laboratory Coordinator
(709) 737-6799 (ofc)
(709) 737-6790 (lab)
(709) 737-6193 (FAX)
{http://www.mun.ca/creait/maf/}
{http://www.esd.mun.ca/epma/}

Inco Centre
c/o Memorial University
230 Elizabeth Avenue
P.O. Box 4200
St. John's, NL A1C 5S7




==============================Original Headers==============================
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==============================Original Headers==============================
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From: brakenho-at-science.uva.nl
Date: Thu, 8 Dec 2005 03:33:15 -0600
Subject: [Microscopy] FOM 2006, Student Bursaries, Focus on Microscopy 2006, Perth,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

---------------------------------------------------------------------- |
FOCUS ON MICROSCOPY 2006 -- Perth, Australia -- April 9-12, 2006 |
----------------------------------------------------------------------

19th International Conference on 3D Image Processing in Microscopy 18th
International Conference on Confocal Microscopy


Dear Colleagues,

We would like to announce that about 15 fee waivers will be available for
students for attending the upcoming Focus on Microscopy conference
courtesy of the generous support of the Australian Microscopy and
Microanalysis Society.

For details see the website http://www.FocusOnMicroscopy.org at "student
bursaries". The deadline for bursary application is Jan. 3, 2006.

As the next in a series of unique interdisciplinary meetings on advanced
multidimensional light microscopy and image processing, the FOM 2006
conference will be hosted by the University of Western Australia in Perth.
The conference will be located at the beautiful Esplanade Hotel in the
historic waterfront suburb of Perth, Fremantle.

Focus on Microscopy 2006 is the continuation of a successful conference
series presenting the latest innovations in optical microscopy and its
applications in biology, medicine, material science, and information
storage. 3D optical imaging and related theory are important subjects for
the conference. The series is as relevant now as at any time in its
history as the scientific and engineering communities strive to meet the
needs of a surging life sciences sector, as well as respond to the
sustained pressure for miniaturization in lithography and data storage.

The conference series is known for covering the rapid development of
advanced fluorescence labeling techniques for the confocal and
multi-photon 3D imaging of -live- biological specimens. This year, in
addition, special attention will be given to imaging in thick tissues and
the use of laser light as an active tool at sub-micrometer length scales
for cell biology, nanobiotechnology, and medicine.

Abstracts for contributions are invited and can already be submitted
through the website: http://www.FocusOnMicroscopy.org where further
information on the present and previous FOM conferences can be found.

Important dates:

- Deadline for the submission of abstracts: January 9, 2006 -
Acceptance of contributions and draft program: January 23, 2006 -
Deadline for early registration: February 20, 2006

Welcoming you to beautiful Perth for the FOM2006 conference and exhibition.

On behalf of the organizing committee,

- David Sampson, University of Western Australia, Perth, Australia - Fred
Brakenhoff, Swammerdam Institute for Life Sciences, University of
Amsterdam, The Netherlands --
E-mail: info2006-at-FocusOnMicroscopy.org
Web: www.FocusOnMicroscopy.org







--
Prof. Dr. G.J. Brakenhoff
Section of Molecular Cytology
Centre for Advanced Microscopy
Swammerdam Institute for Life Sciences
University of Amsterdam
Kruislaan 316
P.O.Box 94062
1090 GB Amsterdam
Tel.: +31 - 20 – 525 5189
Fax.: +31 - 20 - 525 6271
e-mail: brakenhoff-at-science.uva.nl




--
Prof. Dr. G.J. Brakenhoff
Section of Molecular Cytology
Centre for Advanced Microscopy
Swammerdam Institute for Life Sciences
University of Amsterdam
Kruislaan 316
P.O.Box 94062
1090 GB Amsterdam
Tel.: +31 - 20 – 525 5189
Fax.: +31 - 20 - 525 6271
e-mail: brakenhoff-at-science.uva.nl


==============================Original Headers==============================
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From: petra.wahlbring-at-goodyear.com
Date: Thu, 8 Dec 2005 03:36:55 -0600
Subject: [Microscopy] Re: AFM sample prepartion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Shrunali,

I would recommend to use freshly cleaved mica as a substrate in place of a
glass slide. The topography of glass slides can be as large as several 10nm
if not some 100nm. Probably you will not be able to distinguish your
nanopartilces on such a substrate.

Best regards,

Petra

---------------------------------------
Dr. Petra Wahlbring
Goodyear S.A. Technical Center
Analytical Test Laboratories
L-7750 Colmar-Berg
Luxembourg
Tel +352 8199 3725
Fax +352 8199 3905
e-mail: petra.wahlbring-at-goodyear.com




aarti_harle-at-yahoo
.co.in
To
12/07/05 08:46 AM petra.wahlbring-at-goodyear.com
cc

Please respond to Subject
aarti_harle-at-yahoo [Microscopy] AFM sample prepartion
.co.in












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The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Hello

We seek help for the sample prepartion for AFM
ananlysis.
We are trying to scan the suspended nanoparticles in
semicontact mode. Basically We made a smear on glass
slide and observed but unable to obtain the good
pictures.

Thanks in advance

Regards
Shrunali Kulkarni, Scientist
Institute of Microbial Technology
India



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From: Henrik.Kaker-at-guest.arnes.si
Date: Thu, 8 Dec 2005 08:10:10 -0600
Subject: [Microscopy] viaWWW: Image distortion in Jeol JSM 35-CF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
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please copy both Henrik.Kaker-at-guest.arnes.si as well as the MIcroscopy Listserver
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Email: Henrik.Kaker-at-guest.arnes.si
Name: Henrik Kaker

Organization: SEM-EDS Lab

Title-Subject: [Filtered] Image distortion in Jeol JSM 35-CF

Question: Good Morning, All

In our old SEM we found image distortion. Please see the
image of Cu grid at http://www.kaker.com/300x.jpg. Can anyone help me to correct this error in our SEM. Any assistance available is greatly appreciated.

Thanks,

Henrik

---------------------------------------------------------------------------

==============================Original Headers==============================
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From: eschumacher-at-mccrone.com
Date: Thu, 8 Dec 2005 08:41:08 -0600
Subject: [Microscopy] EXTENDED ABSTRACT DEADLINE: MMMS Student Poster Competition, March 24, 2006

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

EXTENDED ABSTRACT DEADLINE FOR STUDENT POSTER COMPETITION

To be held in conjunction with the March 24, 2006 meeting of the Midwest

Microscopy and Microanalysis Society

Affiliate of the Microscopy Society of America and the Microbeam
Analysis
Society of America

NEW ABSTRACT DEADLINE: Abstracts must be received in electronic format
by 5PM, Monday, January 16, 2006.

The first quarter meeting of the Midwest Microscopy and Microanalysis
Society will be held on Friday, March 24, 2006, in the Pancoe Life
Sciences Building, Northwestern University, Evanston, Illinois. A
student poster competition open to undergraduate and graduate students
will be held in
conjunction with the meeting. Posters should illustrate utilization of
microscopy for either biological or materials science study. Prizes
will be awarded as follows:

$300 for 1st place
$200 for 2nd place
$100 for 3rd place

Abstracts must be received in electronic format by 5PM on Monday,
January 16, 2006. To be eligible for a prize, you must be first author
on the poster, and you must be present at the meeting. You are
encouraged to submit your entry as early as possible, as space may be
limited. Abstracts from last year's competition, and an example of the
judging worksheet can be found on the MMMS website:


http://northwestern.edu/bioimaging/MMMS%20Website/index.htm

Further details will be provided upon acceptance of your submission.

Please send the following information to the email address below, and
attach your abstract as a Word document:

Name Phone number
Affiliation Email address
Mailing address

Send to:

Elaine Schumacher
MMMS Materials Science Director
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539
Tel: 630-887-7100 Fax: 630-887-7417
Email: eschumacher-at-mccrone.com



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From: NWWhite-at-bwxt.com
Date: Thu, 8 Dec 2005 08:47:34 -0600
Subject: [Microscopy] viaWWW: Image distortion in Jeol JSM 35-CF

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
X-from: Henrik.Kaker-at-guest.arnes.si [mailto:Henrik.Kaker-at-guest.arnes.si]
Sent: Thursday, December 08, 2005 9:15 AM
To: White, Woody N.

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
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Email: Henrik.Kaker-at-guest.arnes.si
Name: Henrik Kaker

Organization: SEM-EDS Lab

Title-Subject: [Filtered] Image distortion in Jeol JSM 35-CF

Question: Good Morning, All

In our old SEM we found image distortion. Please see the
image of Cu grid at http://www.kaker.com/300x.jpg. Can anyone help me to
correct this error in our SEM. Any assistance available is greatly
appreciated.

Thanks,

Henrik

------------------------------------------------------------------------
---

==============================Original
Headers==============================
9, 12 -- From zaluzec-at-microscopy.com Thu Dec 8 08:10:10 2005
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From: gary-at-gaugler.com
Date: Thu, 8 Dec 2005 10:07:00 -0600
Subject: [Microscopy] Re: viaWWW: Image distortion in Jeol JSM 35-CF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Check that the specimen is grounded. The stage
grounding wire may have come loose or become
broken. Confirm by directly grounding the specimen
holder inside the chamber.

The other cause could be loss of AC line sync.

gary g.


At 06:28 AM 12/8/2005, you wrote:



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From: richard.beanland-at-bookham.com
Date: Thu, 8 Dec 2005 10:43:05 -0600
Subject: [Microscopy] Low energy ion milling

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Hi,
a friend (mhg25-at-cam.ac.uk) is considering purchasing a low energy ion mill (e.g. the 'Gentle Mill', or the Fischione machine). Does anyone have any experience (good or bad) that they would like to share on the technique and the machines, which would help?

Many thanks

Richard

________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


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From: lcgould-at-med.cornell.edu
Date: Thu, 8 Dec 2005 11:18:14 -0600
Subject: [Microscopy] Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
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Hi all,
Nestor, I hope I'm not violating any rules with this...

I just received this message from Sigma Xi:

SURPLUS EQUIPMENT FOR HURRICANE VICTIMS
Do you have any surplus lab or research-related scientific equipment
you could offer to university or other research labs affected by the
hurricanes? You can list it on Sigma Xi Exchange at
http://exchange.sigmaxi.org You can also search for equipment that
has been listed.

Rather than setting up redundant services, perhaps anyone who can
help can use this link.

Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org

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From: larry-at-cymru.freewire.co.uk
Date: Thu, 8 Dec 2005 14:56:21 -0600
Subject: [Microscopy] Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

This is an entirely personal opinion ...

If I am misunderstanding this, I apologise - I'm British and the USA
is a foreign country as far as I'm concerned, with a culture I don't
pretend to fully understand.

And sorry, I'm probably violating list rules, hoiwever, I guess I can
live with being excluded, if Nestor considers it necessary, but what
on earth are colleges and laboratories in the richest and most
powerful nation on the planet doing asking for donations?

Mexico, Cuba, Dominican Republic, Haiti and other Carribean nations,
I could understand but come on, please, the USA, the wealthiest
nation on the planet?

If you've got equipment to donate, send it to somewhere it really is needed.

--
Larry Stoter

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
2. Any mail, legitimate or not, apparently or actually from hotmail,
netscape, yahoo or excite will automatically be deleted.
3. Mail with no subject or without a clear subject will be ignored :-)

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From: nicholls-at-post.queensu.ca
Date: Thu, 8 Dec 2005 15:31:19 -0600
Subject: [Microscopy] EM fees

Contents Retrieved from Microscopy Listserver Archives
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Hi all
I wanted to know what sort of fees various labs charge their users
for services rendered. We are going to be able to offer edx and eels
in the near future and we were wondering what sort of schemes other
labs use for charging in house and external clients. I am wondering
what other labs charge for: straight beam time, specimen prep,
training, pictures (yes we still use film!) edx, eels etc for in
house and external clients and whether some of these things are
lumped together (for instance are pictures covered in the beam time price etc).
Thanks in advance!

Rod Nicholls
Histology/Electron Microscopy Technician
Department of Anatomy and Cell Biology
Queen's University
Kingston, Ontario K7L 3N6
Phone: 613- 533 6000 x 78265


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From: lcgould-at-med.cornell.edu
Date: Thu, 8 Dec 2005 16:15:28 -0600
Subject: [Microscopy] Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
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Larry,
I'm replying off the list so as not to start a flame-throwing
barrage. There may be one anyway, but I won't have contributed to
it....
The area struck by Hurricane Katrina suffered severe damage. All of
Tulane University is being housed by and taught at other
institutions, and that's just one of the schools in the area. Also,
most of the research at those schools is funded by money from the NIH
and other granting institutions. The NIH funds come from US tax
dollars, and the schools will be hard-pressed to replace much of
their equipment when so much of their ready cash must be directed
simply to making the buildings habitable/functional again.
Usually, the US schools are generous about sending excess/older
equipment out of the country. I know that Weill, where I am has sent
things to Columbia and other SA countries in the past. This is a
unique situation. Major portions of 3 states were devastated. To
put it in terms you might better appreciate: imaging England, France
and Germany being wiped out by a natural disaster. Where you guys
have individual nations, we have states.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org

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1, 22 -- Subject: [Microscopy] Re: surplus equipment for hurricane victims
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From: cgarber-at-2spi.com
Date: Thu, 8 Dec 2005 19:19:01 -0600
Subject: [Microscopy] Lab fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Rod Nicholls wrote:
==============================================
Hi all
I wanted to know what sort of fees various labs charge their users
for services rendered. We are going to be able to offer edx and eels
in the near future and we were wondering what sort of schemes other
labs use for charging in house and external clients. I am wondering
what other labs charge for: straight beam time, specimen prep,
training, pictures (yes we still use film!) edx, eels etc for in
house and external clients and whether some of these things are
lumped together (for instance are pictures covered in the beam time price
etc).
Thanks in advance!
==============================================
There are certainly laws in the USA and I believe also in Canada and in most
other countries that would make illegal competitors of a class from sitting
down and discussing their fees and selling prices. Most governemental legal
systems see this as "price fixing". I would respectfully suggest this
should not be an appropriate topic for discussion on this listserver.

A more appropriate kind of discussion might relate to what would and would
not constitute appropriate use of the institutional facilities for external
users. After all, universities should not be in direct competition with
for-profit tax-paying commerical firms. It might happen in certain
university environments, but that does not make it right.

This does not mean that universities should not be doing work for external
users. It only means that such work should be restricted to that which is
consistent with educational objectives, namely that the work is basic and
fundamental in nature, the results would be intended for prompt publication
and the results would be suitable for inclusion in a student's thesis.
Excluded would be work the results from which would become the private
property of the sponsoring customer, would not be intended for prompt
publication and would not be appropriate for inclusion in a student's
thesis.

I would respectfully suggest that discussions about fees among those of us
who privide services for a fee should not be a part of this listserver.

Disclaimer: For the 35+ years of the existence of my firm, I have had to
face unfair competition from certain universities and other tax-exempt
entities. So indeed I do have a vested interest in seeing that unviersities
do not enter the marketplace of services that take unfair advantages of
their tax status. Numerous independent laboratories exist globally to
provide such services in the private sector, for example of some, see URL
http://www.2spi.com/catalog/hot-service7.html

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
Structure Probe, Inc. FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




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From: lcgould-at-med.cornell.edu
Date: Fri, 9 Dec 2005 08:04:06 -0600
Subject: [Microscopy] Re: Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Lee
Some numbers might help.
Germany has only 50% of the land area of Texas (we all know it's big) but
has 30% more population.
The total population of the three countries you mention (191 million) is
about three times the total population of the Gulf states Texas, Missouri,
Georgia,
Louisiana, Alabama, Florida (~64 million), living in about a quarter of the
area.

Chris

----- Original Message -----
X-from: {lcgould-at-med.cornell.edu}
To: {cjeffree-at-staffmail.ed.ac.uk}
Sent: Thursday, December 08, 2005 10:20 PM

OOPS!
I really did think that I'd wiped the list's address from my reply...sorry.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org

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From: DusevichV-at-umkc.edu
Date: Fri, 9 Dec 2005 09:11:33 -0600
Subject: [Microscopy] RE: Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yes of course, apologies for my dyslexia, I meant Mississippi

Setting geography aside, since Lee's cat is now out of the bag on this
question of used/surplus equipment, it might
be of interest to know what aid is being requested and proposed. I expect
many would help out
if they knew how to, and what resources are required.

Chris

----- Original Message -----
X-from: "Debby Sherman" {dsherman-at-purdue.edu}
To: {c.jeffree-at-ed.ac.uk}
Sent: Friday, December 09, 2005 1:40 PM



} This is a
} unique situation. Major portions of 3 states were devastated. To
} put it in terms you might better appreciate: imaging England, France
} and Germany being wiped out by a natural disaster.

It's a long way out of proportions.

Vladimir


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From: gwe-at-ufl.edu
Date: Fri, 9 Dec 2005 09:29:23 -0600
Subject: [Microscopy] Re: Lab fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} This just sounds like a little market research to me.
} ----------------------------------------------------------------------------
}
} Rod Nicholls wrote:
} ==============================================
} Hi all
} I wanted to know what sort of fees various labs charge their users
} for services rendered. We are going to be able to offer edx and eels
} in the near future and we were wondering what sort of schemes other
} labs use for charging in house and external clients. I am wondering
} what other labs charge for: straight beam time, specimen prep,
} training, pictures (yes we still use film!) edx, eels etc for in
} house and external clients and whether some of these things are
} lumped together (for instance are pictures covered in the beam time price
} etc).
} Thanks in advance!
} ==============================================
} There are certainly laws in the USA and I believe also in Canada and in most
} other countries that would make illegal competitors of a class from sitting
} down and discussing their fees and selling prices. Most governemental legal
} systems see this as "price fixing". I would respectfully suggest this
} should not be an appropriate topic for discussion on this listserver.
}
} A more appropriate kind of discussion might relate to what would and would
} not constitute appropriate use of the institutional facilities for external
} users. After all, universities should not be in direct competition with
} for-profit tax-paying commerical firms. It might happen in certain
} university environments, but that does not make it right.
}
} This does not mean that universities should not be doing work for external
} users. It only means that such work should be restricted to that which is
} consistent with educational objectives, namely that the work is basic and
} fundamental in nature, the results would be intended for prompt publication
} and the results would be suitable for inclusion in a student's thesis.
} Excluded would be work the results from which would become the private
} property of the sponsoring customer, would not be intended for prompt
} publication and would not be appropriate for inclusion in a student's
} thesis.
}
} I would respectfully suggest that discussions about fees among those of us
} who privide services for a fee should not be a part of this listserver.
}
} Disclaimer: For the 35+ years of the existence of my firm, I have had to
} face unfair competition from certain universities and other tax-exempt
} entities. So indeed I do have a vested interest in seeing that unviersities
} do not enter the marketplace of services that take unfair advantages of
} their tax status. Numerous independent laboratories exist globally to
} provide such services in the private sector, for example of some, see URL
} http://www.2spi.com/catalog/hot-service7.html
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President
} Structure Probe, Inc. FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.2spi.com
} ########################
} ============================================
}
}
}
}
} ==============================Original Headers==============================
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} 12, 26 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com}
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} ==============================End of - Headers==============================

Greg Erdos
5410 SE 185th Ave
Micanopy, FL 32667


==============================Original Headers==============================
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From: cammer-at-aecom.yu.edu
Date: Fri, 9 Dec 2005 10:45:01 -0600
Subject: [Microscopy] Lab fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } I would respectfully suggest that discussions about fees among those of
} } us
} } who privide services for a fee should not be a part of this listserver.


Many of us run facilities, other shared services or businesses. It is not
collusion to discuss rate structures in public. In fact, it is required
by law that we post our rates in public.

It benefits all of us, including our clients and customers, when we
discuss on this listserv any issues regarding microscopy or providing
microscopy services, especially if they will enhance our knowledge and
ability to provide better services. This helps EVERYBODY.


As a personal aside, it offends my libertarian bent when gov't competes in
the free market, so I sort of agree with the screeds, but I'm really tired
of the anti-university-facility screeds periodically posted to this
listserv. Furthermore, I don't know about the rest of the world, but in
New York it is extremely difficult for a microscopy service to balance a
budget even with subsidization and I don't think there is much of a market
for microscopy outside of universities and corporations that largely have
their own equipment and expertise in house. In other words, this
discussion is hypotheticals and philosophy, not practical concerns.



_________________________________________
Michael Cammer
Analytical Imaging Facility and
Dept. ASB Biophotonics Innovation Laboratory
Albert Einstein College of Medicine
1300 Morris Park Avenue, Bronx, NY 10461
718-430-2890 Fax 718-430-8996
work: http://www.aecom.yu.edu/aif/
personal: http://coxcammer.com/



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From: jae5-at-lehigh.edu
Date: Fri, 9 Dec 2005 12:34:03 -0600
Subject: [Microscopy] Lab fees and outside use of university instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The last time I looked I could not find my copy, but NSF long ago
published guidelines regarding work by federally funded labs for outside
users. I also can not find it on the NSF web site (which seems to have
only much newer stuff).

As I remember them, the NSF guidelines said that any instruments funded
by NSF may be used to perform work for users outside the institution in
which they are located only in the following circumstances:
1 If no commercial analytical laboratory has equipment that can do the job.
2 If no commercial analytical laboratory wishes to do the job.
3 If the samples would be prejudiced by traveling to a commercial
analytical laboratory that is further away.
4 If the work is performed as a genuine research collaboration between
the outside user and the host institution.
and that even if these conditions apply, the rates charged should be
comparable with those of commercial laboratories.

My belief is that DOE refers the people it funds to the NSF guidelines,
so that the guidelines can be considered rather general for all
federally funded equipment. The idea is that any work not covered by
these guidelines is unfair competition for the commercial laboratories.
Note that it is necessary (under these guidelines) to charge as much
as the commercial labs but that in itself is not enough.

For twenty years I have operated under these guidelines (at Illinois and
at Lehigh) and on many occasions I have sent potential users to
commercial labs (and thereby turned down funds that were very much
needed). Even if the NSF document has become lost, I still use the
principles it establishes because they seem well founded.

Note that in Chuck Garber's email he referred only to point 4, above.
There are three other reasons that make it possible to do outside work.
There are some grey areas though. For example, I have allowed outside
work when the outside user insisted in operating the microscope
themselves and the commercial labs would not allow that. Chuck implies
that the research done must be publishable. I do not recall that from
the guidelines, neither does it seem logical. Universities may choose
to do classified research and that should surely be included under point 4.

Still the overall principle stands. Universities should not enter into
unfair competition with commercial analytical laboratories and charging
more than they do is not justification enough for taking on the work

Alwyn Eades

--
...........
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvania 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu


==============================Original Headers==============================
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From: jfactor-at-ns.purchase.edu
Date: Fri, 9 Dec 2005 12:56:25 -0600
Subject: [Microscopy] Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Without commenting on the current discussion, I would like to suggest
that people identify themselves when writing to the list (full name and
affiliation). A first name and an often undecipherable email address do
not tell us who sent a comment or with whom we are conversing.
--Jan Factor

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College, State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



DusevichV-at-umkc.edu wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
6, 19 -- From jfactor-at-ns.purchase.edu Fri Dec 9 12:56:22 2005
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From: bozhilov-at-ucr.edu
Date: Fri, 9 Dec 2005 13:18:06 -0600
Subject: [Microscopy] Lab fees and outside use of university instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would not agree with most opinions expressed here so far.

If an analytical lab in a public university does work for outside
users it SHOULD charge less than the prevailing commercial fees.

If a public university is charging the prevailing commercial rates
then in these rates are included the taxes and benefits a commercial
organization usually charges its customers. Since the public
university lab does not need to pay taxes, benefits etc. it would
generate unjustifiably higher profits if it charges commercial rates
and this is what I would call unfair busines practice.

Krassimir Bozhilov

______________________________________________________
Central Facility for Advanced Microscopy and Microanalysis
University of California
Riverside, CA 92521

tel 951 927 2998
fax 951 827 2489
bozhilov-at-ucr.edu
_______________________________________



==============================Original Headers==============================
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From: pgrover-at-bilbo.bio.purdue.edu
Date: Fri, 9 Dec 2005 13:40:32 -0600
Subject: [Microscopy] Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I tend to listen to what anyone has to say, full professor from prestigeous
university or humble amateur.

Paul Barton Grover, Jr., Ph.D.
Chief Microscopist, Bottle Washer, and Grand Poobah
302 Murphy St.
Lafayette, IN 47905


------------------------------------------------------------
The world is so full of a number of things
I'm sure we should all be as happy as kings

--Robert Louis Stevenson


-----Original Message-----
X-from: jfactor-at-ns.purchase.edu [mailto:jfactor-at-ns.purchase.edu]
Sent: Friday, December 09, 2005 2:04 PM
To: pgrover-at-bilbo.bio.purdue.edu

Without commenting on the current discussion, I would like to suggest
that people identify themselves when writing to the list (full name and
affiliation). A first name and an often undecipherable email address do
not tell us who sent a comment or with whom we are conversing.
--Jan Factor

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College, State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



DusevichV-at-umkc.edu wrote:

} ---------------------------------------------------------------------------
-
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

==============================Original Headers==============================
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From: jquinn-at-www.matscieng.sunysb.edu
Date: Fri, 9 Dec 2005 13:59:08 -0600
Subject: [Microscopy] re: rate structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks

re: rate structure for recharge
and other "off-campus" stuff

You may want to check out the following:
Office of Management Budget (OMB) Circular A-21,
"Cost Principles for Colleges and Universities"
http://www.whitehouse.gov/omb/circulars/a021/a021.html

regards,

Jim


**********************************************************
Dr. Jim Quinn james.quinn-at-stonybrook.edu
Materials Science 631-632-6663 FAX:8052
Stony Brook University www.matscieng.stonybrook.edu
Stony Brook, New York 11794 - 2275
**********************************************************



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10, 12 -- Subject: re: rate structure
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From: DusevichV-at-umkc.edu
Date: Fri, 9 Dec 2005 14:01:41 -0600
Subject: [Microscopy] RE: Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Without commenting on the current discussion, I would like to suggest
} that people identify themselves when writing to the list
} (full name and
} affiliation). A first name and an often undecipherable email
} address do
} not tell us who sent a comment or with whom we are
} conversing. --Jan Factor
}
} ---------------------------------------
} Jan Robert Factor, Ph.D.

My apologies.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
E-mail: DusevichV-at-umkc.edu
Web: http://www.umkc.edu/dentistry/microscopy



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From: as-at-astonmet.com
Date: Fri, 9 Dec 2005 14:15:29 -0600
Subject: [Microscopy] Re: Lab fees and outside use of university

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



If you were a potential user, without regard to legality nor NFS/DOE
restrictions, would you choose the more expensive commercial lab or the
cheaper university lab?

Alan Stone




At 01:41 PM 12/9/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Alan Stone
ASTON Metallurgical Services Co., Inc.
200 Larkin Drive Ste A
Wheeling, IL 60090
847/353-8100
www.astonmet.com


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From: hanke-at-mee-inc.com
Date: Fri, 9 Dec 2005 15:05:34 -0600
Subject: [Microscopy] Re: Lab fees and outside use of university instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} Furthermore, I don't know about the rest of the world, but in
} New York it is extremely difficult for a microscopy service to balance a
} budget even with subsidization and I don't think there is much of a market
} for microscopy outside of universities and corporations that largely have
} their own equipment and expertise in house.

As the manager of a commercial laboratory offering SEM and light
microscopy services, I can assure you that there is a considerable
market for microscopy services outside of universities and large
corporate labs. Our lab has two modern SEMs, one variable pressure and
one field emission, that stay busy enough to be viable as a commercial
enterprise despite (IMHO) unfair competition from federally subsidized
university laboratories.

I understand the need for microscopy services at research universities
and am sympathic to the challenges of maintaining instruments on a
limited budget. But I agree that following the NSF guidelines is the
ethical path for facilities with public funding.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


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From: gary-at-gaugler.com
Date: Fri, 9 Dec 2005 15:11:52 -0600
Subject: [Microscopy] Re: Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jan:

If you look back in time, you will see that when Nestor
changed the filtering scheme on 3 July 2005, people's
names were dropped from the posting header. What was
left was their e-mail address... their "Reply To" address.
Some of these are obvious while some are not. So I suppose
for those that are not obvious what their name is, it is
a good idea to put one's name in their posting. However, if
you look at the bottom of each posting, the old name header
info is still there. Same for you as for Vladimir.

} } 6, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}


gary g.


At 11:23 AM 12/9/2005, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: murraytm-at-u.washington.edu
Date: Fri, 9 Dec 2005 15:42:51 -0600
Subject: [Microscopy] Re: Lab fees and outside use of university instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A couple of points.

My facility does pay state sales tax on all purchases and also
charges state sales tax on any invoices. We also include an overhead
charge for all invoices outside the university.

Even with those additional charges our cost would still be well below
the cost for the same service at a corporation. The main reasons are:

The money to acquire capital equipment is from taxes in the form of
grants. This is money that does not need to be recovered.
Salaries at a public university tend to be somewhat less than in the
corporate world.
We are non-profit and only have to cover out costs.

These items give the university a totally unfair advantage over a for
profit corporation. Another aspect is the unfair situation of the
University using tax money, some of which is paid by the corporation
it would be competing with, to undercut corporations.

Our policy is to charge a substantially larger rate for non-
university work, onto which is added an overhead charge and sales tax.

The possible advantages to using this University's facility would be:

We have equipment that is not available locally.
We are willing to train users and allow them to run the equipment.
We have a pool of knowledge readily available.

Pricing is not an advantage, since we have to charge at least the
going rate in the corporate world.

All that being said, My facility does very little work for non-
university entities.

Tom

On Dec 9, 2005, at 11:33 AM, bozhilov-at-ucr.edu wrote:

}
} I would not agree with most opinions expressed here so far.
}
} If an analytical lab in a public university does work for outside
} users it SHOULD charge less than the prevailing commercial fees.
}
} If a public university is charging the prevailing commercial rates
} then in these rates are included the taxes and benefits a commercial
} organization usually charges its customers. Since the public
} university lab does not need to pay taxes, benefits etc. it would
} generate unjustifiably higher profits if it charges commercial rates
} and this is what I would call unfair busines practice.
}
} Krassimir Bozhilov
}
} ______________________________________________________
} Central Facility for Advanced Microscopy and Microanalysis
} University of California
} Riverside, CA 92521
}
} tel 951 927 2998
} fax 951 827 2489
} bozhilov-at-ucr.edu
} _______________________________________
------------------------------------------------------------------------
---------------------------------------------
Thomas M Murray email:
murraytm-at-u.washington.edu
Electron Microscopy Center Manager Phone: (206)543-2836
Materials Science & Engineering Fax: (206)543-3100
Box 352120 302 Roberts Hall Cell: (425)345-0083
University of Washington
Seattle, WA 98195


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From: kenconverse-at-qualityimages.biz
Date: Fri, 9 Dec 2005 17:13:04 -0600
Subject: [Microscopy] Re: surplus equipment for hurricane victims

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul,
True, but it often helps one to understand the thrust of some of these posts
if you have a better idea of who they are and where they're from.

What I find frustrating is when someone posts a need for service and all
that is on the posting for identification is a name and -at-hotmail.com. Is
this person down the street from me, across the country or are customs and
immigration a major consideration (not to mention export restrictions on
some things to some places)?

Simply having a "signature" automatically appended to your emails takes care
of the problem and keeps everyone informed.

My $0.02

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: pgrover-at-bilbo.bio.purdue.edu [mailto:pgrover-at-bilbo.bio.purdue.edu]
Sent: Friday, December 09, 2005 3:50 PM
To: kenconverse-at-qualityimages.biz

I tend to listen to what anyone has to say, full professor from prestigeous
university or humble amateur.

Paul Barton Grover, Jr., Ph.D.
Chief Microscopist, Bottle Washer, and Grand Poobah
302 Murphy St.
Lafayette, IN 47905


------------------------------------------------------------
The world is so full of a number of things
I'm sure we should all be as happy as kings

--Robert Louis Stevenson


-----Original Message-----
X-from: jfactor-at-ns.purchase.edu [mailto:jfactor-at-ns.purchase.edu]
Sent: Friday, December 09, 2005 2:04 PM
To: pgrover-at-bilbo.bio.purdue.edu

Without commenting on the current discussion, I would like to suggest
that people identify themselves when writing to the list (full name and
affiliation). A first name and an often undecipherable email address do
not tell us who sent a comment or with whom we are conversing.
--Jan Factor

---------------------------------------
Jan Robert Factor, Ph.D.
Professor of Biology
---------------------------------------
Natural Sciences
Purchase College, State University of New York
735 Anderson Hill Rd.
Purchase, NY 10577
USA
---------------------------------------
Office Tel: 914-251-6659
Office Fax: 914-251-6635
E-mail: jfactor-at-ns.purchase.edu
or- jan.factor-at-purchase.edu
---------------------------------------



DusevichV-at-umkc.edu wrote:

} ---------------------------------------------------------------------------
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From: PWebster-at-hei.org
Date: Fri, 9 Dec 2005 17:23:50 -0600
Subject: [Microscopy] Re: Lab fees and outside use of university instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There are many issues being brought up with this seemingly simple subject.
Judging by the number of times the subject gets aired, these issues never
appear to be resolved.

Should universities encourage outside users?
Probably not. Despite the push from administrators to "encourage" EM
facilities to make money, these facilities are being massively subsidize.
Subsidies come from university support for salaries, presumably in the form
of student fees and other income, as well as from government grants awarded
to perform specific research work.

Should all EM users pay the real costs for their work?
In a university setting where the subsidies are in place in part to
encourage faculty members to make use of shared facilities, the answer is
"probably not". Low cost EM availability is often used as a recruiting tool
by universities so it may be unreasonable for users to pay the actual costs
to keep shared imaging facilities running. I am sure that all academic EM
labs would soon disappear if such a charging scale was put in place.

Should EM users know what the real costs are?
Absolutely. As a community we have the responsibility of knowing how much,
in real terms, we are worth. Only then can we start to expect our colleagues
to appreciate the costs of EM. However, government granting systems are such
that there is no built in way of meeting actual costs, hence the concept of
a shared facility.

Personally, I think that university imaging facilities attracting outside,
commercial users is a bad thing. If there is insufficient demand for the
facility within the university, then perhaps a re-evaluation for the need of
such a facility should be made.

Although much blame for academic institutions poaching work from commercial
laboratories can be put on administrative pressures to make money, people in
academic environments should also be responsible enough to admit to some of
the blame. Academic labs should be offering something that is unavailable
elsewhere and is so good that there is a queue of faculty, post-docs and
students waiting outside every morning. I must admit that I see little of
this at the moment.

Regards,

Paul Webster

(and I agree completely that we should place an identifier at the end of
each message - not to be snobbish but to understand a little extra of the
message context)


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
(213) 273 8026
pwebster-at-hei.org



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

} Furthermore, I don't know about the rest of the world, but in
} New York it is extremely difficult for a microscopy service to balance a
} budget even with subsidization and I don't think there is much of a market
} for microscopy outside of universities and corporations that largely have
} their own equipment and expertise in house.

As the manager of a commercial laboratory offering SEM and light
microscopy services, I can assure you that there is a considerable
market for microscopy services outside of universities and large
corporate labs. Our lab has two modern SEMs, one variable pressure and
one field emission, that stay busy enough to be viable as a commercial
enterprise despite (IMHO) unfair competition from federally subsidized
university laboratories.

I understand the need for microscopy services at research universities
and am sympathic to the challenges of maintaining instruments on a
limited budget. But I agree that following the NSF guidelines is the
ethical path for facilities with public funding.

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


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From: gwe-at-ufl.edu
Date: Fri, 9 Dec 2005 18:15:08 -0600
Subject: [Microscopy] Lab fees and outside use of university

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My lab has more than it can handle serving my own institution. The
rare outside contracts come through collaborations with our faculty
or by word of mouth from former students or faculty who have been
pleased with our work. We certainly do not solicit external
users. Our administration has told us that our first obligation is
to our internal users. We get requests for service from around the
country and around the world. For biologists, distance makes the
process extremely difficult and I always encourage folks that they
find resources closer to home, be it in the private sector or at a
University. They are usually persistent in wanting us to do the
work, having spent some effort in an unsuccessful search for services
elsewhere. The outside clients that we do serve are seeking quality
service and technical expertise, but more important to them is the
wide variety of scientific talent that can be tapped at a large
research university. So basically we are in a different kind of
business than the commercial labs.

At 05:34 PM 12/9/2005 -0600, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Interdisciplinary Center for Biotechnology Research
Scientific Director, Electron Microscopy Core Lab
P.O. Box 118525
University of Florida
Gainesville, FL 32611
Phone: 352-392-1295
Fax: 352-846-0251
Email: gwe-at-ufl.edu


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From: cgarber-at-2spi.com
Date: Fri, 9 Dec 2005 22:04:00 -0600
Subject: [Microscopy] More on lab fees and outside users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jim Quinn wrote:
=======================================
Folks
re: rate structure for recharge and other "off-campus" stuff

You may want to check out the following:
Office of Management Budget (OMB) Circular A-21,
"Cost Principles for Colleges and Universities"
http://www.whitehouse.gov/omb/circulars/a021/a021.html
==============================================
I could not find any place in this document where is addressed the issue of
universities competing with for-profit tax-paying entities.

But I would suggest that anyone interested in seeing the divergence in views
between what some believe to be the case vs. what is actually the case,
check out URL
http://prism.mit.edu/nsf.in91/in91.htm

You can see the full text of the original NSF Important Notice 91 and the
updated document that replaced it, NSF Important Notice 122.

And while I realize I am taking it a bit out of context, one quote from
Important Notice 91:
------------------
NSF-supported instrumentation or facilities may be used by or for the
for-profit sector only when such use does not constitute provision of
services equivalent to services available on a commercial basis.
-------------

And a quote from the more recent and current Important Notice 122:
-------------------
It is contrary to the NSF's intent for grantees to use NSF-supported
research instrumentation or facilities to provide
services for a fee in competition with private companies in a manner that is
prohibited by OMB Circular A-110.
------------

Note that NSF is not saying it is OK to compete provided one charges a
commercial rate. Using the instrumentation in competition with private
firms is contrary to NSF intent. That is a line drawn in the sand. But the
documents also recognize, as do commercial laboratories, that there are
times, such as for perishable samples, or a unique instrumentation
capability where such instrumentation use would be acceptable. But ask any
representative from a commercial laboratory, the rub is not with these
almost "outlier" situations, the problem is with the routine repetitive
kinds of inspection and microscopy and failure analysis work that come up
and get done every day.

When a university accepts an NSF grant, their administrators are required to
sign off on a document certifying that they are in compliance with Important
Notice 122 (and before that, Important Notice 91). I would think that
enforcement people at NSF, if any are on this list, would find quite
interesting how some employees of their grantee institutions interpret (and
follow) published NSF guidelines.

Disclaimer: Structure Probe, Inc. is a for-profit tax-paying laboratory
that experiences unfair competition from certain universities on an almost
daily basis so we have a vested interest in seeing that something is done to
reduce its frequency of occurrence.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
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########################
============================================



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From: gary-at-gaugler.com
Date: Sat, 10 Dec 2005 00:20:51 -0600
Subject: [Microscopy] Lab fees and outside use of university

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I was going to let this go but in the spirit of fueling controversy, I congratulate you for doing something I have still not managed to do.

That is to create enough income to pay for my salary, my benefits (retirement and health), the salaries and benefits for all the people working for me, the service contracts for the machines, supplies, utility costs (electrical, water and waste) and the other related costs for the building. Put into that the costs for improving buildings, equipment inventory and repairs, and it is a truly wonderful achievement.

All replies and comments are welcomed because I probably deserve to be told to mind my own business.


Regards,

Paul Webster

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles
CA 90057
(213) 273 8026.


-----Original Message-----
X-from: Lou Ann Miller [mailto:lamiller-at-uiuc.edu]
Sent: Fri 12/9/2005 7:15 PM
To: Webster, Paul

I cannot get any response from universities or businesses
to provide bio hazard human pathogen SEM specimens...dead
of course. I seek specimens that are characteristic of
the pathogens: bacteria, fungi, parasites, that are of
interest to humans. These can be SEM specimens, STEM
specimens or TEM negs. Several postings have resulted in
no responses. So I guess the academic labs cannot offer
anything. And I don't know where else they might be offered.

I am willing to pay for the specimens...no takers. why not?
I don't know why. There are many human-related specimens
that are probably used and dumped. Pity.

In this case, I think that the universities are better set up
and qualified to deal with this sort of material...IMO.
So how does this factor into the fee situation? No product
at any price is quite a different matter.

gary g.




At 03:49 PM 12/9/2005, you wrote:
} [snip]
}
} Although much blame for academic institutions poaching work from commercial
} laboratories can be put on administrative pressures to make money, people in
} academic environments should also be responsible enough to admit to some of
} the blame. Academic labs should be offering something that is unavailable
} elsewhere and is so good that there is a queue of faculty, post-docs and
} students waiting outside every morning. I must admit that I see little of
} this at the moment.
}
} Regards,
}
} Paul Webster


==============================Original Headers==============================
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From: zaluzec-at-aaem.amc.anl.gov
Date: Sat, 10 Dec 2005 10:06:06 -0600
Subject: [Microscopy] NSF Rules on Fees/Charges for Instrument Usage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues:

FYI, here is a brief exerpt from the NSF WWW Site, which you can download
as http://www.nsf.gov/pubs/gc1/gc1_605.pdf

Grant General Conditions (GC-1), June 15, 2005
Document Type: Policies and Procedures
Document Number: gc1605
Document History: Posted June 10, 2005.

========================================================================
" The grantee shall not use equipment acquired
with Federal funds to provide services to
non-Federal outside organizations for a fee that
is less than private companies charge for
equivalent services, unless specifically
authorized by statute in accordance with 2 CFR
§215.34(b). "
========================================================================

Alternatively you can refer to:
http://whitehouse.fed.us/omb/fedreg/2004/040511_grants.pdf
which documents statue 2 CFR §215.34(b). which
says essentially the same thing as the above
NSF quotation.

Of course, the loop holes here are the phrases "
equivalent services", "outside organizations"
and " specifically authorized by statute".

Note this does not prohibit outside (non-Federal
non-organizational) users, it just sets the
boundary conditions on rates which must be
charged. It also does not specify the rates that
you charge internal (organizational) users.

With these points in mind, discussing rates
that are charged to your institutional users and
how one determines those rates is not illegal and
is a valid discussion topic for this listserver.
This does not violate any NSF rules that I
currently know about.

Nestor
Your Friendly Neighborhood SysOp


--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Lab
Electron Microscopy Center
Materials Science Division/Bldg 212
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================


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From: donc-at-asmicro.com
Date: Sun, 11 Dec 2005 22:36:05 -0600
Subject: [Microscopy] Re: rate structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Since 1990, I have operated an independent, for-profit analytical laboratory
specializing in Atomic Force Microscopy. Competition with universities
offering like services at lower than commercial rates has been a serious
problem for us. I would like to comment on a few of the posts in this
discussion.



Jim Quinn of Stony Brook Univ. wrote:

"re: rate structure for recharge and other "off-campus" stuff
You may want to check out the following:

Office of Management Budget (OMB) Circular A-21,
"Cost Principles for Colleges and Universities"
http://www.whitehouse.gov/omb/circulars/a021/a021.html"

--This document is very large. Can you point out the specific sections that
relate to this discussion?



Alwyn Eades, Department of Materials Science and Engineering, Lehigh
University wrote:

"Universities should not enter into unfair competition with commercial
analytical laboratories and charging more than they do is not justification
enough for taking on the work"

--I found this particularly ironic, since one of my prospective customers is
sending routine AFM analyses to Lehigh University. He buys blocks of
instrument time under the guise of supporting Lehigh's research as an
"Industrial Affiliate."



Gregory W. Erdos, Ph.D., of Interdisciplinary Center for Biotechnology
Research, University of Florida wrote:

"My lab has more than it can handle serving my own institution. The
rare outside contracts come through collaborations with our faculty
or by word of mouth from former students or faculty who have been
pleased with our work. We certainly do not solicit external
users. The outside clients that we do serve are seeking quality
service and technical expertise, but more important to them is the
wide variety of scientific talent that can be tapped at a large
research university. So basically we are in a different kind of
business than the commercial labs."

--I submit that the universities providing analytical services are in a
substantially similar business as the commercial labs. The top commercial
lab managers network with other commercial laboratories which offer related
services. In this manner, one lab can manage a project drawing on the wide
variety of scientific talent that exists nationwide and worldwide.

Although Dr. Erdos does not solicit outside work, he does some and thereby
contributes to our problem. My observation of the economics of the
commercial labs providing AFM services is that, as a group, we are suffering
the "death of a thousand cuts". When 1000 university labs take on 1 outside
job a year, this corresponds to 10 full-time analytical scientists in the
commercial labs.

How does my company survive? The answer is superior quality of service.
One of our overseas customers told me he had tried various university labs
in his region of the United Kingdom. He found the AFM at one university was
not working, at another it was not calibrated, at a third there were no
unused probe tips. What we provide our customers is this:
-a working, calibrated instrument

-a plentiful supply of probe tips, with much variety

-unique test methods and know-how

-experienced scientists operate the AFM and write the reports. We know what
a good image looks like so that bad imaging conditions can be corrected "on
the fly". We interpret the images so that the customer gets clear answers
to the questions being investigated.



regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]



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From: ahmadsam-at-sabic.com
Date: Mon, 12 Dec 2005 08:16:15 -0600
Subject: [Microscopy] viaWWW: Locate Analysis Facility

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Email: ahmadsam-at-sabic.com
Name: Shahreer Ahmad

Organization: SABIC Technology Center

Title-Subject: [Filtered] Locate Analysis Facility

Question: We highly apprecite if some can provide us commercial support to analyze following samples by SEM-EDS-WDS-EBSD or TEM.

1. Analyze Mn and C segregation present in steel samples. We tried to analyze the samples by SEM-EDS but unsuccessful due to poor static and low concentration difference between the matrix (0.8%) and the bands (1.2%) and bad contrast in image.

2. Analyze and quantify the phases (Ferrite, Martensite and Bainite) present in steel samples.

Best regards.

Shahreer Ahmad


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From: eggert-at-mikroanalytik.de
Date: Mon, 12 Dec 2005 09:35:14 -0600
Subject: [Microscopy] Re: viaWWW: Locate Analysis Facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Shahreer Ahmad

there you will find a couple of analytical labs, you should go into contact:

http://microanalysis.mikroanalytik.de/service%20microanalysis%20e.phtml#link1

Best regards

F.E.

ahmadsam-at-sabic.com schrieb:

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From: cmderr-at-ucdavis.edu
Date: Mon, 12 Dec 2005 10:26:35 -0600
Subject: [Microscopy] Small Business Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Over here at the University of California at Davis, we have our own
microscopy center (SEMs, TEMs, etc). The administrator running the
facility is trying to get a little more organized in terms of inventory,
purchases, scheduling, etc. I'd written a note to our local computer
list asking if anyone knew of a useful software package for organizing
such a place...Quickbooks, for example, might do the trick. Someone
mentioned there are packages geared specifically towards microscopy
areas, and suggested I ask this list.

So, does anyone have any thoughts or recommendations on software to
handle inventory, purchasing, users, etc? We actually have online
scheduling...this would be more for the money side of things.

Thank you for your time,

Christopher Derr
UC Davis

==============================Original Headers==============================
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From: kenconverse-at-qualityimages.biz
Date: Mon, 12 Dec 2005 11:14:49 -0600
Subject: [Microscopy] viaWWW: Image distortion in Jeol JSM 35-CF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Henrik,
The image shows a problem with orthagonality. I'm assuming that the
specimen is NOT tilted. If that is the case, then an oscilloscope would
show that the X signal has a significant amount of Y summed to it.

I checked my schematics for a 35GF and there doesn't appear to be any
correction adjustment for orthagonality. It would be located in the
magnification section if present.

Do you have a scan rotation and tilt correction module? I don't seem to
have a schematic for it but this module could malfunction in such a way as
to give you exactly what you are seeing. It could be as simple as a dirty
switch or a stuck relay.

Try turning the rotation function on and off, also try rotating the dial and
see if the distortion changes. If it does, the problem is in the scan
rotation, tilt correction module. If the distortion doesn't change, my best
guess is that there is some cross-talk between the X and Y signals in the
vicinity of the magnification section. It could be a cabling error, given
the magnitude of the distortion.

Good luck.

Ken converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


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Email: Henrik.Kaker-at-guest.arnes.si
Name: Henrik Kaker

Organization: SEM-EDS Lab

Title-Subject: [Filtered] Image distortion in Jeol JSM 35-CF

Question: Good Morning, All

In our old SEM we found image distortion. Please see the
image of Cu grid at http://www.kaker.com/300x.jpg. Can anyone help me to
correct this error in our SEM. Any assistance available is greatly
appreciated.

Thanks,

Henrik

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From: jehrman-at-mta.ca
Date: Mon, 12 Dec 2005 12:45:36 -0600
Subject: [Microscopy] JEOL SEM Control User Interface, v. 5.26

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ho-Ho listers,

We've recently upgraded our JEOL 5600 computer and software, now using
v. 5.26 of the GUI. Since it no longer seems fashionable to talk about
"bugs" in
software, I'd be interested in contacting someone with this version of
software to
see if the "feature" I have occurs on every machine, or just a
peculiarity of my upgrade.
This involves instruments that have the PRD (photo recording device)
installed, or at
least with that option enabled in software. Please contact me off-list.

Thanks in advance,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


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From: lkrupp-at-us.ibm.com
Date: Mon, 12 Dec 2005 12:50:04 -0600
Subject: [Microscopy] TEM cross-sections of MgO substrates

Contents Retrieved from Microscopy Listserver Archives
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Hello-

Does anyone have tips for preparing cross-sectional TEM samples of
multi-layers on MgO? These are 1" single crystal wafers. I need to use a
non-FIB method like dimpling or tripod polishing.

Thanks,
Leslie

Leslie Krupp (Thompson)
IBM Almaden Research
650 Harry Road, K19/D2
San Jose, CA 95120-6099


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From: sonia.mato-at-upc.edu
Date: Mon, 12 Dec 2005 15:22:26 -0600
Subject: [Microscopy] viaWWW: magnetic etching

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Email: sonia.mato-at-upc.edu
Name: Sonia Mato DÌaz

Organization: Polytechnic University of Catalonia

Title-Subject: [Filtered] magnetic etching

Question: Hi there,
I am looking for information about magnetic etching and I found in this web site a quite old message about it.
The thing is that I cannot access to the Metals Handbook is recommended there.
I would like to use Ferrofluids EMG 708 and 308 for visualizing the martensite phase transformed in a 304LN at the tip of a fatigue crack. My questions are:
-The sample is electropolished before the fatigue test, do I have to chemically etch the surface around the crack before applying the Ferrofluids?
-How big should be the applied magnetic field to magnetize the martensite before applying the Ferrofluids?
-I have read in a paper I have to dilute the Ferrofluids in water and to add a wetting agent. What is this wetting agent? Which is its function?
Thank you very much,
Sonia

---------------------------------------------------------------------------


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From: jfmjfm-at-umich.edu
Date: Tue, 13 Dec 2005 10:09:58 -0600
Subject: [Microscopy] Sad News - Stanley L. Erlandsen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I apologize if this has been send before, but I just heard of Stan
Erlandsen's death on Monday December 5th 2005.
An MSA supporter and past-president, Stan will be remembered by many
in the microscopy community.
Here is the HCS web page with more details:

http://www.histochemicalsociety.org/members/erlandsen.shtml

--
John Mansfield PhD CPhys CSci MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143 USA
Phone: (734) 936-3352
FAX (734) 763-2282
Cell. Phone: (734) 834-3913
(Leaving a phone message at 936-3352 is preferable to 834-3913)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"
AIM: thejfmjfm
Yahoo: thejfmjfm
Skype: thejfmjfm




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From: donc-at-asmicro.com
Date: Tue, 13 Dec 2005 15:50:14 -0600
Subject: [Microscopy] Imaging Martensite phase (magnetic domains.)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sonia Mato asked about the use of ferrofluid to visualize magnetic phases.
She asked 3 questions. My responses are inserted after each one.
My questions are:
-The sample is electropolished before the fatigue test, do I have to
chemically etch the surface around the crack before applying the
Ferrofluids?
--I think this depends on what resolution you want to achieve when you image
the surface. I assume that the martensite phase is produced by the fatigue
process, not the chemical etch. Therefore, I suggest try first without
etching.
-How big should be the applied magnetic field to magnetize the martensite
before applying the Ferrofluids?
--try a strong disc-shaped permanent magnet, like the rare earth magnets
sold by Edmund Scientific. Try to hold it about 1-2 mm above the sample
surface.
-I have read in a paper I have to dilute the Ferrofluids in water and to add
a wetting agent. What is this wetting agent? Which is its function?
--In our lab we use "Joy" brand liquid dish detergent as the wetting agent
and dilute at least 10x. The purpose is to keep the ferrofluid particles
from clumping together. The particle size might be 50 nm or smaller, which
allows high resolution imaging of magnetic domains by optical microscope and
SEM.
Magnetic domains can be imaged with or without ferrofluid particles using
AFM in Magnetic Force Microscopy mode.
I hope this helps.
regards,
Don Chernoff
==================================
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]


----- Original Message -----
From: sonia.mato-at-upc.edu
To: donc-at-asmicro.com
Sent: Monday, December 12, 2005 4:55 PM
Subject: [a] [Microscopy] viaWWW: magnetic etching





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Email: sonia.mato-at-upc.edu
Name: Sonia Mato DÌaz

Organization: Polytechnic University of Catalonia

Title-Subject: [Filtered] magnetic etching

Question: Hi there,
I am looking for information about magnetic etching and I found in this
web site a quite old message about it.
The thing is that I cannot access to the Metals Handbook is recommended
there.
I would like to use Ferrofluids EMG 708 and 308 for visualizing the
martensite phase transformed in a 304LN at the tip of a fatigue crack. My
questions are:
-The sample is electropolished before the fatigue test, do I have to
chemically etch the surface around the crack before applying the
Ferrofluids?
-How big should be the applied magnetic field to magnetize the martensite
before applying the Ferrofluids?
-I have read in a paper I have to dilute the Ferrofluids in water and to
add a wetting agent. What is this wetting agent? Which is its function?
Thank you very much,
Sonia

--------------------------------------------------------------------------
-


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From: edelmare-at-muohio.edu
Date: Tue, 13 Dec 2005 16:18:06 -0600
Subject: [Microscopy] Ralph knives . . .

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

O.k., here's a shot in the dark. On eof the labs here has been
using Ralph knives with a rotary microtome. (Ralph Knives: The
glass knives broken with a 25mm long edge rather than the 6-9mm
thickness). But now they are trying to cut 1-3um sections, and they
cut fine but the block face scrapes the backside of the knive on the
return stroke. So looking for solutions:

(1) Ultramicrotomes with their "d" motion (backup before return
stroke) work ideally but they do not hold knives large enough for the
desired sections (i.e. ralph knives). Does any one know of a
method of using ralph knives in an ultramicrotome?

(2) It seems once apon a time there was a rotary microtome which
pulled the samples back before the return stroke. Does anyone out
there happen to have such a beast sitting around gathering dust
they would consider oarting with?

(3) Does anyone have any other suggestions?

Thanks!



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"WE ARE MICROSOFT.
RESISTANCE IS FUTILE.
YOU WILL BE ASSIMILATED."

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From: schooley-at-mcn.org
Date: Tue, 13 Dec 2005 17:39:11 -0600
Subject: [Microscopy] Re: Ralph knives . . .

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Richard -

We had our shop machine a more-or-less "T" shaped aluminum block that
clamped in the knife holder of an ancient MT-2. It projected
slightly over the front of the knife holder. We warmed it on a hot
plate & added the Ralph with a bit of dental wax. Worked fine.
Design one!

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html

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4, 17 -- To: edelmare-at-muohio.edu
4, 17 -- From: Caroline Schooley {schooley-at-mcn.org}
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From: richard.beanland-at-bookham.com
Date: Wed, 14 Dec 2005 04:42:49 -0600
Subject: [Microscopy] RE: TEM - replicating tape

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi listers,
I have a contamination issue which I'm thinking of tackling using replication. I know the principles, roughly. There used to be plenty of expertise here when SEMs and AFMs weren't invented and we used tape and TEM to look at surface structure (I still have some lovely images from the 1960's) but it's no surprise that the people with the knowledge are long gone.
So. I still have a packet of replicating tape, the Edwards 12E6 coating unit and a TEM. I have surfaces contaminated with particles a few nm in size - my hope is that they will come off in the replicating tape and be transferred to the carbon film for some nice clean EDX analysis without any ion milling or other specimen prep artefacts. Can anyone give me some advice on how to use the replicating tape? (By the way it is probably about 15 years old, will it still be any good?) And hints on getting the carbon film onto a grid would be good too, I know this is one of those techniques where little tricks make a big difference.

Many thanks in advance

Richard


________________________________________
Richard Beanland
Analytical Services
Bookham Inc
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
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From: edelmare-at-muohio.edu
Date: Wed, 14 Dec 2005 07:30:18 -0600
Subject: [Microscopy] Re: Ralph knives . . .

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you Caroline. I was wondering if something along these
lines would work - nice to know it can work before getting one
made.


On 13 Dec 2005, at 15:34, Caroline Schooley wrote:

} } ---------------------------------------------------------------------
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} }
} } O.k., here's a shot in the dark. On eof the labs here has been
} } using Ralph knives with a rotary microtome. (Ralph Knives: The glass
} } knives broken with a 25mm long edge rather than the 6-9mm thickness).
} } But now they are trying to cut 1-3um sections, and they cut fine but
} } the block face scrapes the backside of the knive on the return
} } stroke. So looking for solutions:
} }
} } (1) Ultramicrotomes with their "d" motion (backup before return
} } stroke) work ideally but they do not hold knives large enough for the
} } desired sections (i.e. ralph knives). Does any one know of a method
} } of using ralph knives in an ultramicrotome?
} }
} } (2) It seems once apon a time there was a rotary microtome which
} } pulled the samples back before the return stroke. Does anyone out
} } there happen to have such a beast sitting around gathering dust they
} } would consider oarting with?
} }
} } (3) Does anyone have any other suggestions?
} }
} } Richard E. Edelmann, Ph.D.
}
} Richard -
}
} We had our shop machine a more-or-less "T" shaped aluminum block that
} clamped in the knife holder of an ancient MT-2. It projected slightly
} over the front of the knife holder. We warmed it on a hot plate &
} added the Ralph with a bit of dental wax. Worked fine. Design one!
}
} Caroline
} --
} Caroline Schooley
} Project MICRO Coordinator
} Microscopy Society of America
} Box 117, 45301 Caspar Point Road
} Caspar, CA 95420
} Phone/FAX (707)964-9460
} Project MICRO: http://www.microscopy.org/ProjectMICRO
} Intertidal invertebrates:
} http://www.fortbragg.k12.ca.us/AG/marinelab.html



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"WE ARE MICROSOFT.
RESISTANCE IS FUTILE.
YOU WILL BE ASSIMILATED."

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From: lesley.bechtold-at-jax.org
Date: Thu, 15 Dec 2005 07:03:24 -0600
Subject: [Microscopy] Position for Light/Confocal Microscopist at The Jackson Laboratory

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Dear Richard,
You do not mention what the surface is that you are removing the particles
from. We have had some success here removing tiny precipitates from steel by
lightly etching the steel, carbon coating it directly, scoring the carbon
coat, etching the steel again and then placing it in distilled water. The
carbon squares float up to be caught on a plain grid and examined in the
TEM. The precipitates are free of the steel matrix for EDX and diffraction.
The replicating tape was used for SEM, but should work the same way. Soften
the tape in acetone and put some acetone on the surface to be replicated.
Place the tape on the surface and wait about half an hour for the tape to
harden up again. Place the tape, surface-of-interest-side up and carbon
coat. I have not tried releasing the carbon film from replicating tape, but
whatever solvent you used to soften it should dissolve it and release the
carbon film.
Good luck.
Regards,
Mary Mager
Electron Microscopist
Department of Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
Tel: 604-822-5648
Fax: 604-822-3619
e-mail: mager-at-interchange.ubc.ca
----- Original Message -----
X-from: {richard.beanland-at-bookham.com}
To: {mager-at-interchange.ubc.ca}
Sent: Wednesday, December 14, 2005 3:26 AM


Light Microscopy Technologist

There is a regular, full-time position available for a Light Microscopy Technologist available in the Light/Confocal Microscopy Service at The Jackson Laboratory in Bar Harbor, ME. Primary duties include direct operation of the various microscope systems, cameras and computer workstations including upright, inverted, confocal, laser capture microdissection and spectral karyotyping systems; providing training for customers in the use of the microscopy equipment; assisting with image capture and analysis; preparing samples for imaging (i.e. immunofluorescent labeling, spectral karyotyping, laser capture microdissection); laboratory maintenance and administrative duties (i.e. ordering supplies, monthly billing).

The position requires a BS in a biological field, preferably with a strong background in genetics and molecular biology with a good working knowledge of microscopy principles and system operations specifically for light, fluorescent and confocal microscopy as well as a strong background in immunohistochemical techniques. A strong proficiency in current computer applications is required, including operational experience with both Macintosh and PCs along with familiarity with standard imaging programs such as Adobe Photoshop and Metamorph. The applicant should have good written and verbal communication skills as well as the ability to work both independently and as part of a team supporting a variety of users in a multi-user core facility. Interested candidates should apply on-line at www.jax.org and refer to job requisition # 0337.


Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322



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From: fvillalovoz-at-deltacollege.edu
Date: Thu, 15 Dec 2005 07:51:32 -0600
Subject: [Microscopy] viaWWW: Job Opening, part-time microscopy instructor

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This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
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Email: fvillalovoz-at-deltacollege.edu
Name: Frank Villalovoz

Organization: San Joaquin Delta College

Title-Subject: [Filtered] Job, part-time microscopy instructor

Question: Electron Microscopy adjunct instructor, one to two courses. Contact Frank Villalovoz at San Joaquin Delta College, Stockton, California Telephone (209) 954-5249 or fvillalovoz-at-deltacollege.edu to obtain more information.

---------------------------------------------------------------------------

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From: hagglundk1-at-nku.edu
Date: Thu, 15 Dec 2005 10:51:11 -0600
Subject: [Microscopy] LM MOTIC inverted scope?

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I was just shown a Motic brand inverted microscope, and wondered whether
anyone on the list has experience with the brand. My first impression
is good, especially on price. The optics seem nice, and construction
sound. Our application would be mainly in undergraduate microbiology
instruction, but also for some research.

Are there any opinions out there? Feel free to reply on or off list.

Karl Hagglund
Biological Sciences, SC-102B
Northern Kentucky University, Nunn Drive
Highland Heights, KY 41099
859-572-5238
http://semlab.nku.edu



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From: mganger-at-optonline.net
Date: Thu, 15 Dec 2005 11:20:00 -0600
Subject: [Microscopy] Mounting Carbon Nanotubes

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Greetings all,

I have to mount SWCNT on formvar grids for TEM examination, I have
small amounts (about 0.1g) in dry form. Any suggestions for mounting
would be most appreciated. If you could reply off list that would be great.

Thanks in advance,


Mike Ganger
Cornell Medical College


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From: gary-at-gaugler.com
Date: Thu, 15 Dec 2005 16:25:03 -0600
Subject: [Microscopy] Residual Stress with EBSD

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Hi Listers:

I was pointed to the NIST site for some info and don't
seem to see it pop out at me. Does anyone have some
pointers for performing residual stress analysis with
TSL EBSD? I'd like to do similar reports to those of
XRD. Pseudo color plots would be ideal along with
quant data.

TIA,
gary g.


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From: bonevich-at-nist.gov
Date: Fri, 16 Dec 2005 08:46:53 -0600
Subject: [Microscopy] Re: Residual Stress with EBSD

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I would consider the following web page for some information:
{http://www.boulder.nist.gov/div853/Program1_Reliability_Dimensionally_Constrained.htm}

Hope this helps (and Happy Holidays)


At 05:33 PM 12/15/2005, gary-at-gaugler.com wrote:



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From: Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Fri, 16 Dec 2005 11:00:16 -0600
Subject: [Microscopy] online image server

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Hi everyone,

Our new Electron Microscopy facility will be hosting two TEM's (Jeol
2200FS, Hitachi HF-3300), two SEM's (Hitachi S4800, S3000N) and maybe a
dual-column FIB. There is also the possibility we will be expanding in
the future.

We are looking for software that allows users to save data from any of
the machines to a local server and be able to retrieve the images from
their own computers (secure online storage). The ideal system would have
search capabilities and would be able to display image previews.

So far, the only suitable software we found is "Quartz PCI" and we want
to analyze all other options.

Thank you again for all your help. This list rocks!

Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



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From: gwe-at-ufl.edu
Date: Fri, 16 Dec 2005 11:21:12 -0600
Subject: [Microscopy] Re: online image server

Contents Retrieved from Microscopy Listserver Archives
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Our folks save images locally and then we post them at an FTP site in
a folder with their name. They can just bring up the site in their
web browser and drag the images to their desktop and save them on
their own site. Some folks want the stuff password protected, but
most people do not care. We leave the images up for one month and
then archive to DVD. So theoretically both the lab and the user have
copies. We used to maintain our own FTP server, but now we use a
departmental server that has a lot more capacity and is maintained by
our IT people.
Others send images directly to their own FTP site and take
us out of the loop. In that case we do not archive and they are
fully responsible for their data.
And yet others will write a DVD or CD before they leave the
lab and take images with them.

At 11:07 AM 12/16/2005 -0600, you wrote:



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5410 SE 185th Ave
Micanopy, FL 32667


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From: tivol-at-caltech.edu
Date: Fri, 16 Dec 2005 11:46:07 -0600
Subject: [Microscopy] Re: online image server

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On Dec 16, 2005, at 9:01 AM, Daniel.Salamon-at-nrc-cnrc.gc.ca wrote:

} We are looking for software that allows users to save data from any of
} the machines to a local server and be able to retrieve the images from
} their own computers (secure online storage). The ideal system would
} have
} search capabilities and would be able to display image previews.
}
Dear Daniel,
Leginon is capable of this and a lot more. Bridget Carragher and
Clint Potter distribute the program and run workshops at The Scripps
Research Institute.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: Jason.Wickersham-at-soft-imaging.net
Date: Fri, 16 Dec 2005 12:55:03 -0600
Subject: [Microscopy] online image server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

---Disclaimer: I represent a commercial interest with this post.----

Soft Imaging System offers this functionality with our software. We
have iTEM for TEM applications, Scandium for SEM applications, and
software for LM applications as well. All versions of the software are
compatible with each other and can write to a central, secure database.

In an effort to keep this from being too commercial, I will respond with
further information off list.

Regards,
Jason Wickersham


Jason Wickersham
Sales Engineer
84 E Grand Avenue
Montvale, NJ 07645
Jason.Wickersham-at-Soft-Imaging.net
551-804-1845


-----Original Message-----
X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca
[mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca]
Sent: Friday, December 16, 2005 12:50 PM
To: Jason Wickersham

Hi everyone,

Our new Electron Microscopy facility will be hosting two TEM's (Jeol
2200FS, Hitachi HF-3300), two SEM's (Hitachi S4800, S3000N) and maybe a
dual-column FIB. There is also the possibility we will be expanding in
the future.

We are looking for software that allows users to save data from any of
the machines to a local server and be able to retrieve the images from
their own computers (secure online storage). The ideal system would have
search capabilities and would be able to display image previews.

So far, the only suitable software we found is "Quartz PCI" and we want
to analyze all other options.

Thank you again for all your help. This list rocks!

Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116
Street Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



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From: xin-at-magnet.fsu.edu
Date: Fri, 16 Dec 2005 13:38:27 -0600
Subject: [Microscopy] problem with PGT pure Ge EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I am a bit confused on whether the Ge EDS detector can have the warming up
cycle or not. Our detector is dead after one warming up. What is the
mechanism of the failure if it warms up? I was never told that the
detector should never be warmed up by the manufacturer technical
support. Is it a common knowledge that Ge detector should NEVER be
warmed up once it is cold?

Thanks

Yan Xin, Ph.D
National High Magnetic Field Laboratory
Tallahassee, FL 32310


==============================Original Headers==============================
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From: r.sims-at-auckland.ac.nz
Date: Fri, 16 Dec 2005 14:02:12 -0600
Subject: [Microscopy] Re: problem with PGT pure Ge EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Is it dead, or is it just the vacuum? I had a PGT Si(li) detector that PGT told
me could safely be warmed up for the Christmas holidays, so, after switching off
the HV, I did so. However, on re-cooling a couple of weeks later, the vacuum had
drastically deteriorated to the extent that the detector had to be re-pumped. It
may be that some detectors, or some manufacturer's detectors, are more likely to
be damaged by warming, regardless of the manufacturer's claims or advice.

cheers

rtch






Quoting xin-at-magnet.fsu.edu:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi,
}
} I am a bit confused on whether the Ge EDS detector can have the warming up
} cycle or not. Our detector is dead after one warming up. What is the
} mechanism of the failure if it warms up? I was never told that the
} detector should never be warmed up by the manufacturer technical
} support. Is it a common knowledge that Ge detector should NEVER be
} warmed up once it is cold?
}
} Thanks
}
} Yan Xin, Ph.D
} National High Magnetic Field Laboratory
} Tallahassee, FL 32310
}
}
} ==============================Original Headers==============================
} 5, 22 -- From xin-at-magnet.fsu.edu Fri Dec 16 13:38:26 2005
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-------------------------------------------------
This mail sent through University of Auckland
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From: kayton-at-ohsu.edu
Date: Fri, 16 Dec 2005 15:44:56 -0600
Subject: [Microscopy] Anti-Roll Plate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I am in search of a solution to replace the anti-roll plate for my
Leitz Kryostat 1720 cryostat. There is no source for new ones, does
anyone have a solution? source? Know a guy? etc.

thanks




==============================Original Headers==============================
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From: xin-at-magnet.fsu.edu
Date: Fri, 16 Dec 2005 16:09:55 -0600
Subject: [Microscopy] Re: problem with PGT pure Ge EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It will be interesting to hear what the manufacturers have to say
officially about this. Meanwhile, I can share our story.

We had a Ge detector on an Oxford EDS system. It had served us well for
years, but it appears that increasing LN2 consumption got ahead of us on
one long interval between fills and the detector warmed up - at least
some. We got it cooled down again and tried it out. There were peaks
where they were supposed to be, but there was also a lot of intensity
tailing off to the low energy side. I suppose that the crystal broke
during warm up and we were having difficulty collecting all the energy
from the x-rays and it was a function of the relation between where they
arrived and the fracture(s) in the crystal. We had to trash that crystal
and replace it.

Bottom line - I would not try warming up any detector without the
express guarantee of the vendor that it won't be damaged in the process.


Warren Straszheim
Iowa State University

-----Original Message-----
X-from: xin-at-magnet.fsu.edu [mailto:xin-at-magnet.fsu.edu]
Sent: Friday, December 16, 2005 1:48 PM
To: wesaia-at-iastate.edu

Hi, Jim,

HT/HV is working, but it does seem the vacuum isn't as good as
before. Usually the TEM column vacuum is below 1x10-4 after boiling off
LN2 using ACD, but now it is 2x10-4. I don't know whether it is due to the
detector.

The detector behaves like this with all the right HT, vacuum, beam current.
When I start to collect spectrum by pressing the start button on the imix,
it immediately gives 100% deadtime.
Maybe I should check the counts.

Regards
Yan Xin

At 04:39 PM 12/16/2005, you wrote:
} Xin Yan
}
} I doubt that one warming cycle would
} intentionally kill a EDS detector,
} whether it is SiLi, GeLi, or another.
}
} It was probably a fluke.
}
} Even if the HV/HT was on, the
} detector would not fail. Instead,
} it would be inaccurate, once recooled.
}
}
} Did you check that the HT/HV is working?
}
} Have you check the counts on an oscope?
}
} How about the vacuum in the dewar?
}
} regards,
}
} Jim
}
}
} } From mail-at-ns.microscopy.com Fri Dec 16 14:46:46 2005
} } Date: Fri, 16 Dec 2005 13:47:47 -0600
} } To: jquinn-at-www.matscieng.sunysb.edu
} } From: xin-at-magnet.fsu.edu
} } Reply-to: xin-at-magnet.fsu.edu
} } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com}
} } Subject: [Microscopy] problem with PGT pure Ge EDS detector
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} }
} ----------------------------------------------------------------------------
} }
} } Hi,
} }
} } I am a bit confused on whether the Ge EDS detector can have the
} warming up
} } cycle or not. Our detector is dead after one warming up. What is the
} } mechanism of the failure if it warms up? I was never told that the
} } detector should never be warmed up by the manufacturer technical
} } support. Is it a common knowledge that Ge detector should NEVER be
} } warmed up once it is cold?
} }
} } Thanks
} }
} } Yan Xin, Ph.D
} } National High Magnetic Field Laboratory
} } Tallahassee, FL 32310
} }
} }
} } ==============================Original
} Headers==============================
} } 5, 22 -- From xin-at-magnet.fsu.edu Fri Dec 16 13:38:26 2005
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} } 5, 22 -- To: microscopy-at-microscopy.com
} } 5, 22 -- From: Yan Xin {xin-at-magnet.fsu.edu}
} } 5, 22 -- Subject: problem with PGT pure Ge EDS detector
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From: rickmott-at-alumni.princeton.edu
Date: Fri, 16 Dec 2005 16:49:17 -0600
Subject: [Microscopy] problem with PGT pure Ge EDS detector

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xin-at-magnet.fsu.edu wrote:
}
}
} The detector behaves like this with all the right HT, vacuum, beam current.
} When I start to collect spectrum by pressing the start button on the imix,
} it immediately gives 100% deadtime.

You don't by any chance have an IR camera on
in the chamber, do you? Detectors have no
sense of humor about infrared.

Rick Mott

==============================Original Headers==============================
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From: ahlst007-at-umn.edu
Date: Fri, 16 Dec 2005 17:50:59 -0600
Subject: [Microscopy] problem with PGT pure Ge EDS detector

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Yan Xin,

As a general comment, I have had Si detectors
warm up now and then, never during actual use
though, as I always check LN levels before
turning it on. I then re-cool the detector and
don't turn the power on until the next day, just
to be safe, and get normal functioning back.

More pertinent to your observation below of 100%
dead time, one time after a warm-up and
subsequent cooling down, I too saw the high
deadtime, due to very high noise count, as it
turned out. Of course, I "flipped out" thinking
the detector was damaged! But eventually I
realized that the calibration of the energy axis
had "slipped" to the very low energy side of the
axis and I was picking up very high noise counts
from that region of the energy axis of 1 to
about 0.4 KeV. This very low energy region of
the system is usually blocked out by the
baseline setting for normal functioning. After
new calibration of energy axis using copper
(just nicking the edge of a copper grid with the
e-beam to get Cu-L xrays at low energy end and
Cu-K xrays at high energy end), using my
system's calibration program, the noise went
away and normal functioning was restored.

So I suggest trying to recalibrate your energy
axis. Maybe that will work for you too.

Good luck!

Gib Ahlstrand
Imaging Center
University of Minnesota
St. Paul, MN



xin-at-magnet.fsu.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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}
} Hi, Jim,
}
} HT/HV is working, but it does seem the vacuum isn't as good as
} before. Usually the TEM column vacuum is below 1x10-4 after boiling off
} LN2 using ACD, but now it is 2x10-4. I don't know whether it is due to the
} detector.
}
} The detector behaves like this with all the right HT, vacuum, beam current.
} When I start to collect spectrum by pressing the start button on the imix,
} it immediately gives 100% deadtime.
} Maybe I should check the counts.
}
} Regards
} Yan Xin

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From: pollingmel-at-verizon.net
Date: Mon, 19 Dec 2005 08:03:17 -0600
Subject: [Microscopy] viaWWW: Microscope as telescope Hydrogen Alpha filter

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Does anyone know how to make a filter that will eject all wavelengths of light from IR through UV, except for the hydrogen alpha band?

Mel Pollinger
New York Microscopical Society

==============================Original Headers==============================
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From: dwaugh-at-kent.edu
Date: Mon, 19 Dec 2005 08:04:33 -0600
Subject: [Microscopy] AskAMicroscopist: SEM sample replication

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Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (dwaugh-at-kent.edu) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, December 18, 2005 at 20:52:20
---------------------------------------------------------------------------

Email: dwaugh-at-kent.edu
Name: David Waugh

Organization: Kent State University

Education: Graduate College

Location: Kent Ohio

Question: SEM sample replication

First I want to thank all the people who have helped me in the past,
I have not always thanked them individually via email, but people on
this list have been exceedingly helpful.

I am beginning a project that will involve the replication of fossil
and rock surfaces for examination under the SEM (gold coated, in most
cases well under 1000X). I was going to try and use a replication
compound made by Struers (Repliset) and a dental casting compound
Examix NDS (Hydrophilic Vinyl Polysiloxane). For making positive
replicas I was going to try Epothin epoxy and SPI positive replica
powder (polyethylene homopolymer), and the casting compounds
themselves. Does anyone have experience using any of these compounds
or know of better alternatives? The casts need to be flexible so that
they can be removed from undercuts. The epoxy I have read about is
Araldite, I could not find out what model number they used, or if it
is some kind of special epoxy? Thanks, Have a good holiday!
-David

David A. Waugh
Kent State University
Department of Geology
Kent, Ohio 44242
dwaugh-at-kent.edu
Http://www.personal.kent.edu/~dwaugh/

---------------------------------------------------------------------------

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From: oshel1pe-at-cmich.edu
Date: Mon, 19 Dec 2005 09:14:48 -0600
Subject: [Microscopy] viaWWW: Microscope as telescope Hydrogen Alpha filter

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Do you need to make one? Why not just buy a H-alpha filter from one of the ads in "Astronomy" or "Sky and Telescope"? Or, a store that sells telescopes to amateur astronomers.
They're relatively cheap, and can have sharp cut-offs.


Philip Oshel
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576




-----Original Message-----
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Sent: Mon 05/12/19 10:11
To: Oshel, Philip Eugene


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Does anyone know how to make a filter that will eject all wavelengths of light from IR through UV, except for the hydrogen alpha band?

Mel Pollinger
New York Microscopical Society

==============================Original Headers==============================
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From: hanke-at-mee-inc.com
Date: Mon, 19 Dec 2005 16:52:43 -0600
Subject: [Microscopy] Re: AskAMicroscopist: SEM sample replication

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David:

I have used both the Repliset and dental casting coupounds extensively
and with good success. I believe that the repliset is a silicone
material and it stays very flexible. A positive can be made with
Repliset on a Repliset original replica if you don't let the polymer
cure too long. Most of the other replicating materials that I have used
cannot be used to make a positive from a like material. The Repliset
material is very easy to work with in that it comes with a very nice
mixing dispenser. The down side of the Repliset is a relatively high cost.

The dental casting compounds and similar products sold as machinist mold
making compounds (ReproRubber Thin Pour Metrology Grade) also work well
and are much cheaper. I have done comparisons using SEM and quantitative
surface measurements of Repliset and ReproRubber and found good
correlations with the original surfaces and the replicas for features in
the size range that you would be seeing at 1000X.

I have also used acrylic compounds, but this material would be too stiff
and brittle for replicating surfaces with undercuts. I expect that
Epothin epoxy may have the same problem, but I have never used this
material or other epoxies for replicas. Acrylics also have a problem
with beam damage in the SEM. Some of these materials, such as the
silicones, will outgas so watch your total replica size, especially if
you will be using a high vacuum SEM.

Hope this helps.

} I am beginning a project that will involve the replication of fossil
} and rock surfaces for examination under the SEM (gold coated, in most
} cases well under 1000X). I was going to try and use a replication
} compound made by Struers (Repliset) and a dental casting compound
} Examix NDS (Hydrophilic Vinyl Polysiloxane). For making positive
} replicas I was going to try Epothin epoxy and SPI positive replica
} powder (polyethylene homopolymer), and the casting compounds
} themselves. Does anyone have experience using any of these compounds
} or know of better alternatives? The casts need to be flexible so that
} they can be removed from undercuts. The epoxy I have read about is
} Araldite, I could not find out what model number they used, or if it
} is some kind of special epoxy? Thanks, Have a good holiday!
} -David
}

--
Larry D. Hanke, P.E.
Materials Evaluation and Engineering, Inc.
Practical Solutions Through Technology and Innovation
http://www.mee-inc.com (763) 449-8870


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From: rbeavers-at-mail.smu.edu
Date: Mon, 19 Dec 2005 17:07:45 -0600
Subject: [Microscopy] Looking for Auger, XPS, or TOF SIMS access in Dallas Area

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Group,

May be a little off target with this group but looking for labs in the Dallas area that could help with some thin film (200A) analysis.

Looking for possible TiN or SiN film contamination on a Al bond pad.

Thanks

Roy Beavers

Southern Methodist University
Department of Geological Sciences
P.O. Box 750395
Dallas, TX 75275
Voice: 214-768-2756
Fax: 214-768-2701
Email: rbeavers-at-smu.edu



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From: rk.tiwari-at-ncl.res.in
Date: Tue, 20 Dec 2005 18:06:05 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: ultramicrotomy questions

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Email: rk.tiwari-at-ncl.res.in
Name: rajkiran

Organization: national chemical laboratory

Title-Subject: [Filtered] ultramicrotomy

Question: Hi all,
I am Rajkiran R Tiwari from NCL, Pune.
I am doing microtoming mostly on polymer samples at room temp. I have some doubts which I want to clarify.
I am mentioning them below:
1. I usually keep diamond knife at 5 deg. whether it is ok??
2. Even after fine polishing the samples with glass knife, the sections with diamond knife doesn't come uniformly.. Like in one round full trapezoid will get and in next cutting only part of trapezoid will cut. What may be reason for it?
3. I cut the sample 100nm and below with diamond knife. Should I polish the sample again with diamond knife at 500nm to make tarpezoid surface uniform??
4. Which side of grid should be used to to collect sample, since in literature people use generally shiny side. Does mess also affect the imaging. Is 400 mess grid is ok to collect the sections?

Kindly help me by clarifying the doubts.

with regards
Rajkiran

---------------------------------------------------------------------------

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From: W.Muss-at-salk.at
Date: Wed, 21 Dec 2005 01:31:26 -0600
Subject: [Microscopy] AW: Re: ultramicrotomy questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning, good afternoon, good evening (as applicable)

Dear Listers, ( see also general informations on SCUR-Meeting 2006 below )

dear R. R. Tiwari,

I am not able now to comment on your questions because it might need some
time to formulate "reasons" (there are some) why you are facing the
problems you describe.
Unfortunately I am not aware of a publication in English commenting
generally on } Ultramicrotomy, frequent problems and faults/imperfections {.

I only do have and could provide you with a 12 pages GERMAN publication on
that, written by one of the "fathers" of modern ultramicrotomy, H. SITTE
(January 1982: Ultramikrotomie - Haeufige Probleme und Fehler, GIT Verlg
Darmstadt ).
If you would like to have a .pdf of that publication, please let me know.

All the best wishes to you and yours,
MERRY CHRISTMAS/Seasons Greetings and
A HAPPY, HEALTHY and PROSPEROUS NEW YEAR 2006

best regards

Wolfgang MUSS
Salzburg, Austria
( http://www.salzburgcb.com/en/salzburg/salzburg.htm )

I am still alive but again struggling and fighting now for the further
existence of the EM-Lab here (February 2nd 2006 it still will have operated
25 years).

OR Dr. Wolfgang Muss Member of MSA, SUP, SCUR, FRMS.....
EM-Lab
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively
Paracelsus Medical Private University (PMU)
Institute of Pathology - Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o
W.Muss")
E-Mail work: W.Muss-at-SALK.at

Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at

----------------------------------------------------------------------
-------
Information on behalf of
Society for Cutaneous Ultrastructure Research (SCUR)
PLEASE VISIT THE UPDATED WEBSITE of SCUR at
} http://www.scur.org.pl {
-------------------------------------------------------------------------
Forthcoming Meetings:
33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland
Additional Informations: send an E-Mail
kwoznia-at-amwaw.edu.pl

34th Annual Meeting of the SCUR, 11th-12th May, 2007, PRAGUE
Czech Republic

35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan
Joint Meeting with the
JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology


----------
Von: rk.tiwari-at-ncl.res.in[SMTP:rk.tiwari-at-ncl.res.in]
Antwort an: rk.tiwari-at-ncl.res.in
Gesendet: Mittwoch, 21. Dezember 2005 01:50
An: W.Muss-at-salk.at
Betreff: [Microscopy] [Filtered] ultramicrotomy questions

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The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Title-Subject: [Filtered] ultramicrotomy

Question: Hi all,
I am Rajkiran R Tiwari from NCL, Pune.
I am doing microtoming mostly on polymer samples at room temp. I have some
doubts which I want to clarify.
I am mentioning them below:
1. I usually keep diamond knife at 5 deg. whether it is ok??
2. Even after fine polishing the samples with glass knife, the sections
with diamond knife doesn't come uniformly.. Like in one round full
trapezoid will get and in next cutting only part of trapezoid will cut.
What may be reason for it?
3. I cut the sample 100nm and below with diamond knife. Should I polish the
sample again with diamond knife at 500nm to make tarpezoid surface
uniform??
4. Which side of grid should be used to to collect sample, since in
literature people use generally shiny side. Does mess also affect the
imaging. Is 400 mess grid is ok to collect the sections?

Kindly help me by clarifying the doubts.

with regards
Rajkiran

---------------------------------------------------------------------------

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==============================Original Headers==============================
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From: yjzhang-at-ciac.jl.cn
Date: Wed, 21 Dec 2005 08:41:57 -0600
Subject: [Microscopy] AskAMicroscopist: Standardless EDS Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (yjzhang-at-ciac.jl.cn) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, December 21, 2005 at 08:08:46
---------------------------------------------------------------------------

Email: yjzhang-at-ciac.jl.cn
Name: Yuanjian Zhang

Organization: Chinese Academy of Sciences

Education: Graduate College

Location: Changchun, Jilin, P.R. China

Question: Hi,

I want to quantify the elements (standardless) from EDAX spectra (created by GENESIS Software, EDAX, Inc in .spc format). But EDAX, Inc only supplies free Spectrum Viewer software, which can not do quantification. I am outside of my institute now, and then I wonder is there another way to quantify from these spectra by myself?

Thanks a lot :)

Best regards!

Yuanjian
yjzhang-at-ciac.jl.cn

---------------------------------------------------------------------------

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From: Daniel.Salamon-at-nrc-cnrc.gc.ca
Date: Wed, 21 Dec 2005 09:57:55 -0600
Subject: [Microscopy] RE: online image server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to thank everybody for their help. I will probably try to
contact each of the responders off the list for details after the
holidays.

Have a very happy holiday season!

Daniel Salamon
Technical Officer, Electron Microscopy

National Institute for Nanotechnology, NRC
W6-017A ECERF Bldg, 9107-116 Street
Edmonton, AB. T6G 2V4

Phone: Office (780) 492 8878
Lab (780) 492 8872
DocuFax: (780) 492 8632



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From: Paul.Perkes-at-asu.edu
Date: Wed, 21 Dec 2005 11:38:47 -0600
Subject: [Microscopy] Death of John C. Wheatley

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

I regret to inform you that John C. Wheatley, lab manager for the John
M. Cowley Center for High Resolution Electron Microscopy at Arizona
State University, died on Sunday, December 18th, 2005. John worked in
the field of electron microscopy for over 35 years and was a long-time
member of this list.

A Celebration & Memorial Service for John Wheatley will be held at

First Baptist Church of Tempe
4525 S McClintock Dr
Tempe, AZ 85282
(480) 839-0926
SE Corner of McClintock and US-60

On Thursday, December 22nd, at 7:00 PM

Flowers to the above or
Mrs. Peggy Wheatley
1139 W Madero Circle
Mesa, AZ 85210


e-Paul
Paul R. Perkes
Principal Technical Support Analyst
Arizona State University
Center for Solid State Science
Phone: (480) 965-5218
Wireless: (602) 999-4781
E-mail: paul.perkes-at-asu.edu



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From: nairvinods-at-gmail.com
Date: Wed, 21 Dec 2005 11:48:38 -0600
Subject: [Microscopy] Re: [Filtered] MicroscopyListserverviaWWW: ultramicrotomy questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Rajkiran,
Here are my thoughts

1. Diamond knife angle
Most diamond knifes have a setting of 4 degress, but then again Ihave
seen a variation in this angle. The setting is usually mentioned on
the original dimond knife box.
2. Glass knife and incomplete trapezoids
Are polishing your block face with the glass knife at the same angle
as that of the diamond knife? If not, that might be the reason for
you incomplete sections
3. Grid surface
It is different for different grids.If you are using a formvar coated
grid then you collect sections on the shiny side, while using a copper
grid (uncoated) you collect on the dull side.

Hope that was helpful.
regards,
Vinod Nair
Graduate Student
Dept. of Biology
New mexico State University

On 12/20/05, rk.tiwari-at-ncl.res.in {rk.tiwari-at-ncl.res.in} wrote:
}
}
}
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}
} Email: rk.tiwari-at-ncl.res.in
} Name: rajkiran
}
} Organization: national chemical laboratory
}
} Title-Subject: [Filtered] ultramicrotomy
}
} Question: Hi all,
} I am Rajkiran R Tiwari from NCL, Pune.
} I am doing microtoming mostly on polymer samples at room temp. I have some doubts which I want to clarify.
} I am mentioning them below:
} 1. I usually keep diamond knife at 5 deg. whether it is ok??
} 2. Even after fine polishing the samples with glass knife, the sections with diamond knife doesn't come uniformly.. Like in one round full trapezoid will get and in next cutting only part of trapezoid will cut. What may be reason for it?
} 3. I cut the sample 100nm and below with diamond knife. Should I polish the sample again with diamond knife at 500nm to make tarpezoid surface uniform??
} 4. Which side of grid should be used to to collect sample, since in literature people use generally shiny side. Does mess also affect the imaging. Is 400 mess grid is ok to collect the sections?
}
} Kindly help me by clarifying the doubts.
}
} with regards
} Rajkiran
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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} 8, 12 -- From: rk.tiwari-at-ncl.res.in (by way of MicroscopyListserver)
} 8, 12 -- Subject: [Filtered] MicroscopyListserverviaWWW: ultramicrotomy questions
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From: nicholls-at-post.queensu.ca
Date: Thu, 22 Dec 2005 09:44:14 -0600
Subject: [Microscopy] Permount and FITC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Anyone ever have problems using permount and FITC? I was asked if
there was any issues about transmitability or other problems that
could arise. I had no evidence to support my premise but it seemed
that to me that the index of refraction shouldn't present a problem
so it should work?

Happy Holidays to all you Listers!

Histology/Electron Microscopy Technician
Department of Anatomy and Cell Biology
Queen's University
Kingston, Ontario K7L 3N6
Phone: 613- 533 6000 x 78265


==============================Original Headers==============================
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From: M_Jarnik-at-fccc.edu
Date: Thu, 22 Dec 2005 10:02:57 -0600
Subject: [Microscopy] His Tag antibody for EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Just wonder if anyone has a tip on a good (commercially available)
antibody against His tag for EM - Tokuyasu technique. Should tolerate at
least light (0.1-0.2%) glutaraldehyde fixation. A rabbit polyclonal
would be preferable, but a working monoclonal would do as well.

Another unrelated question - I would need to label macrophages in
sections (again, Tokuyasu). Any idea for a good macrophage marker?

Thanks and happy Holidays,

Michal


==============================Original Headers==============================
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From: beth-at-plantbio.uga.edu
Date: Thu, 22 Dec 2005 10:21:03 -0600
Subject: [Microscopy] bulb for cryostat

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,
I have a Reichert-Jung Frigocut 2800 cryostat and I need to replace the
light bulb.

Radium
Relux 11W/21
Germany 2x4

Haven't had any luck doing a google search so any advice would be
greatly appreciated.
happy holidays,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



==============================Original Headers==============================
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From: phillipst-at-missouri.edu
Date: Thu, 22 Dec 2005 10:21:34 -0600
Subject: [Microscopy] Re: Permount and FITC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

One doesn't generally used organic solvent based mounting media with
fluorochrome labeled specimens. The xylene in Permount would destroy the
fluorescence. You need one of the many commerical preparations (e.g.,
Prolong Gold from Molecular Probes) or Mowiol (google for the recipe on
line). Good luck.

At 09:54 AM 12/22/05, you wrote:



} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu



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From: rk.tiwari-at-ncl.res.in
Date: Fri, 23 Dec 2005 00:39:53 -0600
Subject: [Microscopy] thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for all the suggestions!
Calling companies worked the best rather than trying websites.
The folks at Bulbman were very helpful.
I appreciate the advice!
best,
Beth

**********************************************************************
Beth Richardson
EM Lab Coordinator
Plant Biology Department
University of Georgia
Athens, GA 30602-7271

Phone - (706) 542-1790 & FAX - (706) 542-1805
http://www.plantbio.uga.edu/emlab

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
*******************************************************************

"And it's only the giving that makes you what you are".
Wond'ring Aloud, Jethro Tull (Aqualung)

************************************************************************
***



==============================Original Headers==============================
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From slinternational-at-hotmail.com Thu Dec 22 17:36:48 2005
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Hi thanks to all for their valuable suggestions.

rajkiran


*****************************************************************
This email is virus free by TrendMicro Inter Scan Security Suite.
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From: cgarber-at-2spi.com
Date: Sun, 25 Dec 2005 16:26:52 -0600
Subject: [Microscopy] Replicating systems for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

David Waugh wrote:
=====================================================
I am beginning a project that will involve the replication of fossil
and rock surfaces for examination under the SEM (gold coated, in most
cases well under 1000X). I was going to try and use a replication
compound made by Struers (Repliset) and a dental casting compound
Examix NDS (Hydrophilic Vinyl Polysiloxane). For making positive
replicas I was going to try Epothin epoxy and SPI positive replica
powder (polyethylene homopolymer), and the casting compounds
themselves. Does anyone have experience using any of these compounds
or know of better alternatives? The casts need to be flexible so that
they can be removed from undercuts. The epoxy I have read about is
Araldite, I could not find out what model number they used, or if it
is some kind of special epoxy?
=====================================================
The selection of a replication system depends on your application.

For example,
a) ultrafast cure can be important, such as for the replication of human
skin (and resolution is less important)
b) high resolution characteristics of the replication resin can be the most
important
c) the surface chemical characteristics and adhesion characteristics can be
important so that when the replica is removed, it does not remove part of
the sample with it.
d) dimensional integrity of the replica can be important as is needed in
dentistry but not necessarily so much in microscopy
d) robust enough so that when a positive replica is made, fine features on
the silicone don't get pulled off by the positive when separated.

For the replication of geological surfaces, we have found the SPI "Wet
Replica" kit to work nicely, but there are trade offs. You will get less
resolution in the negative (vs. epoxy), but when it lifts off, it is the
least likely of the possibilities to remove surface debris and fine features
just barely hanging onto the surface. In order to get the replicating
silicone "into" the sample, we sometimes use a "duster" to sort of help
blast the liquid into the crevices, etc. Now obviously there are limits to
what one can do, even with this particular silicone, but it seems to work
quite well. But since the silicone at that point and in its cured state,
might be tissue-paper thin, very fragile and hard to handle, there is
nothing that prevents one from adding a "second stage', that is, another
layer of the resin to give the high resolution "film" more dimensional
rigidity and overall making it much less fragile. Since the resin is white,
the picking off of pieces from the sample which usually show up "black" can
be used as an assessment as to how much surface material did get removed by
the resin. You can also look at the generally dark colored or black
geological sample and see how much "white" is showing up as a measure of how
much of the replica could not be pulled off of the surface.

The downside to the SPI wet replica kit is that after about 700x in the SEM,
one starts to see artifact structure from the replicating resin itself.

Other information about the SPI Wet Replica Kit can be found at URL
http://www.2spi.com/catalog/spec_prep/wet_rep_kits.shtml

We like the low MW polyolefin "positive" replicating powder which leads to a
good positive replica of the surface. It also separates very easily from
the silicone negative, even when the positive material has to go into small
crevices and convolutions on the negative replica surface.

Disclaimer: SPI Supplies manufactures the SPI Wet Replica Kit.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================





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From: mganger-at-optonline.net
Date: Sun, 25 Dec 2005 19:00:48 -0600
Subject: [Microscopy] Carbon NanoTubes Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Listers,


Thank you to all of you who responded to my inquiry. The general
response was that you can mount them by mixing either an
ethanol/methanol mixture or distilled water with the dry tubes and mount
them either on lacey film or formvar, with the majority suggesting lacey
film.

I tried both and had the best success with the formvar. Thank you all
who gave your suggestions, they were invaluable.

Mike Ganger
Weill Cornell Medical College


==============================Original Headers==============================
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From: elir-at-uiuc.edu
Date: Tue, 27 Dec 2005 14:57:10 -0600
Subject: [Microscopy] Polylysine slides and coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
We are trying to do Ecoli fixate.
I am looking for a procedure of how to make
polylysine coverslips and slides.
I would also like to know if these are commercially
available.

Thanks

Eli

==============================Original Headers==============================
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From: lubo-at-berkeley.edu
Date: Wed, 28 Dec 2005 08:00:14 -0600
Subject: [Microscopy] viaWWW: Trasmitted light in scanning mode: Zeiss LSM510 (meta)

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Email: lubo-at-berkeley.edu
Name: Lubo

Organization: UCB

Title-Subject: [Filtered] Trasmitted light in scanning mode: Zeiss LSM510 (meta)

Question: Hello,
I'd like to get a brightfield image - polarized light - subsequently after a confocal image is taken from the same specimen. Trivial enough.

Here is the problem: the Zeiss LSM510 (meta) we have just shuts the transmitted light off once the lever is pulled all the way out (in the LSM - scanning mode).
According to the manual having the transmitted light on while acquiring an image is a feasible thing to do.
The light path is set correctly I think: Microscope Control panel } transmitted light On, Field Stop iris needs & Filter at 100%, tried with variety of transmitted light settings from 100-0%.

I wonder if shutting the transmitted light off once the light pass lever is pulled out for the scanning mode is a software bug or there is some obscure setting I'm missing?

Thank you for your help.


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From: cni-at-UDel.Edu
Date: Wed, 28 Dec 2005 09:05:19 -0600
Subject: [Microscopy] ZEISS EM-900 TEM for good home

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This is a message posted at the request of a colleague.

A ZEISS EM-900 TEM is available for a good home that probably just needs
to cover the cost for dismantling and shipping.

For more information, please contact Dr. Shu-Chun Su at

SSu-at-Herc.com
(302) 995-3498 (phone)


****************************************
Chaoying Ni
The W.M. Keck Electron Microscope Facility
Facility location: 022 Spencer Laboratory
Mailing address:
201 duPont Hall
Dept of Matls Sci & Eng
University of Delaware
Newark, DE 19716
(302) 831-2318(L); -4545(Fax)
http://eml.masc.udel.edu
*****************************************

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From: microscopytoday-at-tampabay.rr.com
Date: Thu, 29 Dec 2005 16:59:07 -0600
Subject: [Microscopy] Microscopy Today January 2006 Table of Contents

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Listers,

Here is the January 2006 Microscopy Today table of contents. I will
close the subscription list for this issue on Tuesday, January 3, 2006.

Microscopists in North America and MSA members anywhere may have free
subscriptions. Anyone else may subscribe for US$50 per year (to
PARTIALLY cover postage). All subscriptions at
http://www.microscopy-today.com Thank you.

Ron Anderson, Editor
===================
Why Flies Walk with Wet Feet
Stephen W. Carmichael, Mayo Clinic

Advanced Confocal Microscopy An Essential Technique for Microfluidics
Development
Terence Lundy, Hyphenated-Systems, Burlingame, CA

The Staining of Polymers II
R. W. Smith and V. Bryg,* Lake Havasu City, AZ and *Richfield, OH

Low Voltage FESEM of Geological Materials
C. Ma and G. Rossman, California Institute of Technology, Pasadena, CA

Temperature Monitoring of an EM Environment
D. Fellmann, R. Bañez, B. Carragher and C. S. Potter, The Scripps
Research Institute, La Jolla, CA

Practical Issues for Quantitative X-ray Microanalysis in SEM at Low kV
Peter Statham, Oxford Instruments Analytical Limited, High Wycombe,
Bucks U.K.

Mounting Media and Antifade Reagents
Compiled by Tony J. Collins, Wright Cell Imaging Facility, Toronto
Western Research Institute, Canada

Ethics and Digital Imaging
J. M. Mackenzie, M. G. Burke, T. Carvalho and A. Eades, MSA Sub
Committee on the Ethics of Digital Imaging

Investigating the Microstructure of a Newly Developed Aluminum Alloy
Through X-ray Microanalysis
P. Camus and D. Rohde, Thermo Electron Corporation, Madison, WI

Fostering LIMS Development Through Open Standards: Part II – Ontologies
and Business Process
Avrum Goodblatt, PathBioResource, U. PENN School of Medicine

Freezing Biological Samples
Charles W. Scouten & Miles Cunningham, myNeuroLab.com, St. Louis, MO

Industry News

NetNotes


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From: susan.vanhorn-at-stonybrook.edu
Date: Thu, 29 Dec 2005 20:54:47 -0600
Subject: [Microscopy] viaWWW: Potassium Permanganate

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Email: susan.vanhorn-at-stonybrook.edu
Name: Sue Van Horn

Organization: SUNY-at-StonyBrook

Title-Subject: [Filtered] Potassium Permanganate

Question: I am looking for a protocol using potassium permanganate to enhance membrane contrast...any suggestions???
thanks
sue

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From: W.Muss-at-salk.at
Date: Fri, 30 Dec 2005 06:30:10 -0600
Subject: [Microscopy] Re: Potassium Permanganate

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Good morning listers,

dear Sue,
unfortunately you did not specify wether you look for a fixation OR a
staining protocol nor which tissue type (human, animal, plant tissue) you
are going to investigate.
May I add some Infos for you, hoping that you will communicate the answer
results of your questions to me too. Thank you in advance for this.

Therefore I cite: PLATTNER H., ZINGSHEIM H.P: Elektronenmikroskopische
Methodik in der Zell- und Molekularbiologie (GERMAN, G. FISCHER,
Stuttgart, 1987),
ISBN 3-437-30494-1, pp. 41 ff, 282, 284

Version 1).
by KMnO4-Fixation: unfortunately I do not have at hand a protocol which was
described for human tissue fixation.....but - meanwhile I was writing this
message, my PC-"search programme" has found some references and articles
dealing with KMnO4 either as fixation or as contrast/staining agent, at
least one in
NetNotes in Micr. Today, 13, No 3, p. 66 - 67 (May 2005), a word.doc I
could send to you and everybody requesting such documents.

} } translated { {
Permanganate-ions (Mn(VII)O -4) react similarly like OsO4. KMnO4 as a
fixative was introduced by LUFT 1956 for animal tissue and MOLLENHAUER 1959
for EM of plant cells and tissues.
The origial fixation technique still is in use with quite good results for
plant tissue, since the fixative penetrates the cell wall very well leaving
the cells undestroyed by the cell wall structure.
Animalic cells and tissues most often will be destroyed or at least be
damaged by KMnO4 especially if one does not take special care in the
fixation procedure [ no specification given which parameters therefore are
important ].
Ribosomes under any circumstances will be destroyed completely. On the
other hand, KMnO4 produces well stained "unit-membranes" without the
necessity of a further staining the ultrathin sections.
The "term" } } unit-membrane { { originally was deduced from permanganate-fixed
material (ROBERTSON 1958)

KMnO4-FIXATION (MOLLENHAUER 1959) for plant tissue
Pretreatment: reduce size of specimen to 0.3 mm x 0.3mm x 0.3 mm
Method: fixative consists of 2-5 % KMno$-solution, either unbuffered or
buffered with Veronal-Acetate to a pH of 6.0. Fixation at 0?C or room
temperature, for some minutes up to 2 h.
Tips & Results: heavy membrane contrast. Secondary staining usually not
necessary. Ribosomes totally are destroyed/unvisible. For washing one
should use either buffer solution or 25% dehydration medium, e.g. Aceton.
Fixation for animal tissue possible, but sometime this results in destroyed
overall ultrastructural preservation of cellular structures.

Version 2) KMnO4 as a staining agent for ultrathin sections
} } BRAY & WAGENAAR (1978) have reinstated the section staining by
permanganate. The combination with alkaline Pb-citrate section staining
will result in well stained biomembranes.
Method (BRAY and WAGENAAR 1978):
1% unbuffered KMnO4 (hydrous) solution to be applicated 5-20 min (-at-room
temperature = RT). Important to know: grids with sections should be
immersed in the solution by pushing them in a vertical orientation through
the KMnO4-solution's surface
[my personal note: cave: surface tension, perhaps formvar mounting will be
destroyed].
For LowicrylK4M sections, Plattner&Zingsheim recommend [article
KELLENBERGER et al. 1980, PLT-progressive lowering temperature-technique
for L-4KM] also 1% (w/vol) hydrous KMnO4 for 5-10 min -at- RT
After incubation pull out grids also in a vertical orientation, wash
thoroughly with/in A. bidest and counterstain with alkaline Pb-citrate
(e.g. Reynolds, Venable&Coggeshall).

The complete bibliographic references as cited here you can get on request.

Hope that you will be lucky with your results

ALL BEST WISHES for a HAPPY, HEALTHY, PROSPEROUS and SUCCESSFUL
NEW YEAR 2006

best regards,

Wolfgang MUSS
SALZBURG, AUSTRIA

OR Dr. Wolfgang Muss Member of MSA, FRMS....
EM-Lab
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE
----------- and/or/alternatively -------------
Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o
W.Muss")
E-Mail work: W.Muss-at-SALK.at
Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at
------------------------------------------------------------------------
-------------------------------
Ankuendigung namens der (Information on behalf of)
Society for Cutaneous Ultrastructure Research (SCUR)
PLEASE VISIT THE UPDATED WEBSITE of SCUR at
} http://www.scur.org {
-------------------------------------------------------------------------
Forthcoming Meetings:
33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland
WEBSITE, containing all FORMS: http://www.scur.org.pl
Additional informations: send an E-Mail
kwoznia-at-amwaw.edu.pl

34th Annual Meeting of the SCUR, 11th-12th May, 2007, PRAGUE
Czech Republic

35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan
Joint Meeting with the
JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology


----------
Von: susan.vanhorn-at-stonybrook.edu[SMTP:susan.vanhorn-at-stonybrook.edu]
Antwort an: susan.vanhorn-at-stonybrook.edu
Gesendet: Freitag, 30. Dezember 2005 04:34
An: W.Muss-at-salk.at
Betreff: [Microscopy] Potassium Permanganate

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Question: I am looking for a protocol using potassium permanganate to
enhance membrane contrast...any suggestions???
thanks
sue

---------------------------------------------------------------------------

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From: ramadanhany-at-gmail.com
Date: Fri, 30 Dec 2005 10:10:45 -0600
Subject: [Microscopy] Tantalum polishing

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Good morning,

I grow tantalum oxide electrochemically on tantalum substrates, the
issue is that using SEM I can say that the surface of tantalum oxide
is full of defects "pin holes". I just use mechanical polishing of
tantalum before growing oxide, so I think that these defects result
from impurities of polishing sand papers. Is there any other way I can
polish tantalum to the finest grade avoiding these impurities?

I appreciate your responses.

Thanks
--
**********************************************************
Hany Ramadan
Graduate student
Chemistry department
McMaster university, Hamilton, Ontario, Canada
905-525-9140 x: 26322
elsayeh-at-mcmaster.ca
**********************************************************


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From: smalinskas-at-yahoo.com
Date: Fri, 30 Dec 2005 12:12:36 -0600
Subject: [Microscopy] Re: Tantalum polishing

Contents Retrieved from Microscopy Listserver Archives
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Hany:

Pure Tantalum is soft and generally difficult to
polish metallographically. Embedment of abrasive
particles is one of the problems.

First, can you verify the problem of particle
embedment by examining the surface after polishing and
before growing the oxide?... either with a
metallograph or using SEM/EDS?

Though I've personally never polished Tantalum, my
source, "Metallography Principles and Practise" by
George Vander Voort, recommends using a chemical
attack-polish after diamond polishing to prepare soft
Tantalum surfaces.

A number of recipes are offered:

1.

15 g fine alumina
35 ml water
5 ml 20% CrO3 in water
napped cloth, 1750 rpm
medium to heavy pressure
recharge periodically

2.

Solution 1
2-5% aq. CrO3
alumina abrasive

Solution 2
50 ml lactic acid
30 ml HNO3
2 ml HF

napped cloth, 1750 rpm with solution 1
follow with chemical polishing with solution 2

3.

100 ml acetic acid
60 ml HNO3
3 ml HF
alpha alumina
polish through 6 micron diamond
add solution to napped cloth, add dry abrasive
use 30 psi pressure for 8 min
then 10 psi for 1 min

Stu Smalinskas, P.E.
Metallurgist
SKF
Plymouth, Michigan
(734) 414-6862

--- ramadanhany-at-gmail.com wrote:
}
} Good morning,
}
} I grow tantalum oxide electrochemically on tantalum
} substrates, the
} issue is that using SEM I can say that the surface
} of tantalum oxide
} is full of defects "pin holes". I just use
} mechanical polishing of
} tantalum before growing oxide, so I think that these
} defects result
} from impurities of polishing sand papers. Is there
} any other way I can
} polish tantalum to the finest grade avoiding these
} impurities?
}
} I appreciate your responses.
}
} Thanks
} --
}
**********************************************************
} Hany Ramadan
} Graduate student
} Chemistry department
} McMaster university, Hamilton, Ontario, Canada
} 905-525-9140 x: 26322
} elsayeh-at-mcmaster.ca
}
**********************************************************
}




__________________________________
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17, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com}
17, 20 -- Subject: Re: [Microscopy] Tantalum polishing
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From: larry-at-cymru.freewire.co.uk
Date: Sat, 31 Dec 2005 02:57:15 -0600
Subject: [Microscopy] Re: Tantalum polishing

Contents Retrieved from Microscopy Listserver Archives
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1. 90% sulphuric, 10% hydrofluoric acid (40%), electropolish with C
or Pt cathode at 12 to 20 V.

2. 5% sulphuric, 1.25% hydrofluoric acid (40%), 93.75% methanol,
electropolish at 50-70 V.

3. 50% nitric, 50% hydrofluoric (48%), immersion chemical polish.


--
Larry Stoter

PLEASE NOTE
1. Any mail other than plain text will be automatically deleted.
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7, 16 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk}
7, 16 -- Subject: Re: [Microscopy] Tantalum polishing
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